LOCUS       DAJTYD010000001        89985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTYD010000001 DAJTYD010000000
VERSION     DAJTYD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.001"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..791
                     /locus_tag="OO585_000001"
     CDS             <1..791
                     /locus_tag="OO585_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6627727.1"
                     /translation="DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQT
                     GVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGVWVDGMGSPSTGD
                     FHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITY
                     DCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQ
                     NGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO585_000002"
     CDS             complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO585_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6627728.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1758..3785
                     /gene="lpoA"
                     /locus_tag="OO585_000003"
     CDS             1758..3785
                     /gene="lpoA"
                     /locus_tag="OO585_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6627729.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3743..4138
                     /locus_tag="OO585_000004"
     CDS             3743..4138
                     /locus_tag="OO585_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6627730.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4158..4748
                     /gene="diaA"
                     /locus_tag="OO585_000005"
     CDS             4158..4748
                     /gene="diaA"
                     /locus_tag="OO585_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6627731.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4758..5333
                     /gene="yraP"
                     /locus_tag="OO585_000006"
     CDS             4758..5333
                     /gene="yraP"
                     /locus_tag="OO585_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6627732.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5453..6004)
                     /locus_tag="OO585_000007"
                     /pseudo
     CDS             complement(5453..6004)
                     /locus_tag="OO585_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(6077..6712)
                     /locus_tag="OO585_000008"
     CDS             complement(6077..6712)
                     /locus_tag="OO585_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6627733.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6840..7358
                     /gene="yhbO"
                     /locus_tag="OO585_000009"
     CDS             6840..7358
                     /gene="yhbO"
                     /locus_tag="OO585_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6627734.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7338..7781)
                     /locus_tag="OO585_000010"
     CDS             complement(7338..7781)
                     /locus_tag="OO585_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6627735.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO585_000011"
     CDS             7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO585_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6627736.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8121..8624)
                     /locus_tag="OO585_000012"
     CDS             complement(8121..8624)
                     /locus_tag="OO585_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6627737.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8618..9142)
                     /locus_tag="OO585_000013"
     CDS             complement(8618..9142)
                     /locus_tag="OO585_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6627738.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9351..10331
                     /locus_tag="OO585_000014"
     CDS             9351..10331
                     /locus_tag="OO585_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6627739.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10340..11218
                     /locus_tag="OO585_000015"
     CDS             10340..11218
                     /locus_tag="OO585_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6627740.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11424..12431
                     /locus_tag="OO585_000016"
     CDS             11424..12431
                     /locus_tag="OO585_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6627741.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO585_000017"
     CDS             complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO585_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6627742.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO585_000018"
     CDS             complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO585_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6627743.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO585_000019"
     CDS             complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO585_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6627744.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO585_000020"
     CDS             complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO585_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6627745.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO585_000021"
     CDS             complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO585_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6627746.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO585_000022"
     CDS             complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO585_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6627747.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO585_000023"
     CDS             complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO585_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6627748.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO585_000024"
     CDS             complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO585_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6627749.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OO585_000025"
     CDS             complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OO585_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6627750.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREA
                     AEKDKVSNQQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMA
                     EENKWTDNAEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKH
                     AESKADREEARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMA
                     VKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTG
                     AAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITF
                     LDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKI
                     DKPEADPDRVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELK
                     AVRKGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQE
                     VLEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKL
                     ENMFANMTEGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETD
                     ATLAAASNAILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPEL
                     KQQIIGLAEVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRF
                     KDDVNEVRNGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OO585_000026"
     CDS             complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OO585_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6627751.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OO585_000027"
     CDS             complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OO585_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6627752.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26214..26290)
                     /locus_tag="OO585_000028"
     tRNA            complement(26214..26290)
                     /locus_tag="OO585_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26254..26256),aa:Met,seq:cat)
     gene            26638..27981
                     /gene="argG"
                     /locus_tag="OO585_000029"
     CDS             26638..27981
                     /gene="argG"
                     /locus_tag="OO585_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6627753.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(27937..29613)
                     /locus_tag="OO585_000030"
     CDS             complement(27937..29613)
                     /locus_tag="OO585_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6627754.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPLKGRVDVELLAFVFHILHLRHA"
     gene            complement(30072..30158)
                     /locus_tag="OO585_000031"
     tRNA            complement(30072..30158)
                     /locus_tag="OO585_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30122..30124),aa:Leu,seq:gag)
     gene            complement(30173..30505)
                     /gene="secG"
                     /locus_tag="OO585_000032"
     CDS             complement(30173..30505)
                     /gene="secG"
                     /locus_tag="OO585_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6627755.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30733..32070)
                     /gene="glmM"
                     /locus_tag="OO585_000033"
     CDS             complement(30733..32070)
                     /gene="glmM"
                     /locus_tag="OO585_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6627756.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32063..32911)
                     /gene="folP"
                     /locus_tag="OO585_000034"
     CDS             complement(32063..32911)
                     /gene="folP"
                     /locus_tag="OO585_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6627757.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33001..34935)
                     /gene="ftsH"
                     /locus_tag="OO585_000035"
     CDS             complement(33001..34935)
                     /gene="ftsH"
                     /locus_tag="OO585_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6627758.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35035..35664)
                     /gene="rlmE"
                     /locus_tag="OO585_000036"
     CDS             complement(35035..35664)
                     /gene="rlmE"
                     /locus_tag="OO585_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6627759.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35790..36083
                     /gene="yhbY"
                     /locus_tag="OO585_000037"
     CDS             35790..36083
                     /gene="yhbY"
                     /locus_tag="OO585_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6627760.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36240..36716)
                     /gene="greA"
                     /locus_tag="OO585_000038"
     CDS             complement(36240..36716)
                     /gene="greA"
                     /locus_tag="OO585_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6627761.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36964..38397
                     /gene="dacB"
                     /locus_tag="OO585_000039"
     CDS             36964..38397
                     /gene="dacB"
                     /locus_tag="OO585_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6627762.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38437..39609)
                     /gene="cgtA"
                     /locus_tag="OO585_000040"
     CDS             complement(38437..39609)
                     /gene="cgtA"
                     /locus_tag="OO585_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6627763.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39625..40590)
                     /locus_tag="OO585_000041"
     CDS             complement(39625..40590)
                     /locus_tag="OO585_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6627764.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40717..40974)
                     /gene="rpmA"
                     /locus_tag="OO585_000042"
     CDS             complement(40717..40974)
                     /gene="rpmA"
                     /locus_tag="OO585_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6627765.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40995..41306)
                     /gene="rplU"
                     /locus_tag="OO585_000043"
     CDS             complement(40995..41306)
                     /gene="rplU"
                     /locus_tag="OO585_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6627766.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41565..42536
                     /gene="ispB"
                     /locus_tag="OO585_000044"
     CDS             41565..42536
                     /gene="ispB"
                     /locus_tag="OO585_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6627767.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42764..43042
                     /gene="sfsB"
                     /locus_tag="OO585_000045"
     CDS             42764..43042
                     /gene="sfsB"
                     /locus_tag="OO585_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6627768.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43090..44349)
                     /gene="murA"
                     /locus_tag="OO585_000046"
     CDS             complement(43090..44349)
                     /gene="murA"
                     /locus_tag="OO585_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6627769.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44404..44658)
                     /gene="ibaG"
                     /locus_tag="OO585_000047"
     CDS             complement(44404..44658)
                     /gene="ibaG"
                     /locus_tag="OO585_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6627770.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44818..45111)
                     /gene="mlaB"
                     /locus_tag="OO585_000048"
     CDS             complement(44818..45111)
                     /gene="mlaB"
                     /locus_tag="OO585_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6627771.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45111..45746)
                     /gene="mlaC"
                     /locus_tag="OO585_000049"
     CDS             complement(45111..45746)
                     /gene="mlaC"
                     /locus_tag="OO585_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6627772.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45765..46316)
                     /gene="mlaD"
                     /locus_tag="OO585_000050"
     CDS             complement(45765..46316)
                     /gene="mlaD"
                     /locus_tag="OO585_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6627773.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46321..47103)
                     /gene="mlaE"
                     /locus_tag="OO585_000051"
     CDS             complement(46321..47103)
                     /gene="mlaE"
                     /locus_tag="OO585_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6627774.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47111..47920)
                     /gene="mlaF"
                     /locus_tag="OO585_000052"
     CDS             complement(47111..47920)
                     /gene="mlaF"
                     /locus_tag="OO585_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6627775.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGCAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48130..49107
                     /locus_tag="OO585_000053"
     CDS             48130..49107
                     /locus_tag="OO585_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6627776.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49121..50107
                     /gene="kdsD"
                     /locus_tag="OO585_000054"
     CDS             49121..50107
                     /gene="kdsD"
                     /locus_tag="OO585_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6627777.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50128..50694
                     /gene="kdsC"
                     /locus_tag="OO585_000055"
     CDS             50128..50694
                     /gene="kdsC"
                     /locus_tag="OO585_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6627778.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50691..51266
                     /gene="lptC"
                     /locus_tag="OO585_000056"
     CDS             50691..51266
                     /gene="lptC"
                     /locus_tag="OO585_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6627779.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51235..51792
                     /gene="lptA"
                     /locus_tag="OO585_000057"
     CDS             51235..51792
                     /gene="lptA"
                     /locus_tag="OO585_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6627780.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51799..52524
                     /gene="lptB"
                     /locus_tag="OO585_000058"
     CDS             51799..52524
                     /gene="lptB"
                     /locus_tag="OO585_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6627781.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52572..54005
                     /gene="rpoN"
                     /locus_tag="OO585_000059"
     CDS             52572..54005
                     /gene="rpoN"
                     /locus_tag="OO585_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6627782.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54028..54315
                     /gene="hpf"
                     /locus_tag="OO585_000060"
     CDS             54028..54315
                     /gene="hpf"
                     /locus_tag="OO585_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6627783.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54433..54924
                     /gene="ptsN"
                     /locus_tag="OO585_000061"
     CDS             54433..54924
                     /gene="ptsN"
                     /locus_tag="OO585_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6627784.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54970..55824
                     /gene="rapZ"
                     /locus_tag="OO585_000062"
     CDS             54970..55824
                     /gene="rapZ"
                     /locus_tag="OO585_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6627785.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55821..56093
                     /gene="npr"
                     /locus_tag="OO585_000063"
     CDS             55821..56093
                     /gene="npr"
                     /locus_tag="OO585_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6627786.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56307..56939
                     /gene="yrbL"
                     /locus_tag="OO585_000064"
     CDS             56307..56939
                     /gene="yrbL"
                     /locus_tag="OO585_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6627787.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56936..57664)
                     /gene="mtgA"
                     /locus_tag="OO585_000065"
     CDS             complement(56936..57664)
                     /gene="mtgA"
                     /locus_tag="OO585_000065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6627788.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57661..58314)
                     /gene="elbB"
                     /locus_tag="OO585_000066"
     CDS             complement(57661..58314)
                     /gene="elbB"
                     /locus_tag="OO585_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6627789.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58544..60880)
                     /gene="arcB"
                     /locus_tag="OO585_000067"
     CDS             complement(58544..60880)
                     /gene="arcB"
                     /locus_tag="OO585_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6627790.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60976..61905)
                     /locus_tag="OO585_000068"
     CDS             complement(60976..61905)
                     /locus_tag="OO585_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6627791.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62580..67040
                     /gene="gltB"
                     /locus_tag="OO585_000069"
     CDS             62580..67040
                     /gene="gltB"
                     /locus_tag="OO585_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6627792.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67053..68471
                     /gene="gltD"
                     /locus_tag="OO585_000070"
     CDS             67053..68471
                     /gene="gltD"
                     /locus_tag="OO585_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6627793.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68655..69134
                     /locus_tag="OO585_000071"
     CDS             68655..69134
                     /locus_tag="OO585_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6627794.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            69151..69456
                     /locus_tag="OO585_000072"
     CDS             69151..69456
                     /locus_tag="OO585_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6627795.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(69532..69996)
                     /locus_tag="OO585_000073"
     CDS             complement(69532..69996)
                     /locus_tag="OO585_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6627796.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(69993..70868)
                     /gene="nanK"
                     /locus_tag="OO585_000074"
     CDS             complement(69993..70868)
                     /gene="nanK"
                     /locus_tag="OO585_000074"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6627797.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(70865..71554)
                     /locus_tag="OO585_000075"
     CDS             complement(70865..71554)
                     /locus_tag="OO585_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6627798.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71602..73092)
                     /gene="nanT"
                     /locus_tag="OO585_000076"
     CDS             complement(71602..73092)
                     /gene="nanT"
                     /locus_tag="OO585_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6627799.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73201..74094)
                     /gene="nanA"
                     /locus_tag="OO585_000077"
     CDS             complement(73201..74094)
                     /gene="nanA"
                     /locus_tag="OO585_000077"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6627800.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74216..75007)
                     /gene="nanR"
                     /locus_tag="OO585_000078"
     CDS             complement(74216..75007)
                     /gene="nanR"
                     /locus_tag="OO585_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6627801.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75387..76753
                     /gene="dcuC"
                     /locus_tag="OO585_000079"
                     /pseudo
     CDS             75387..76753
                     /gene="dcuC"
                     /locus_tag="OO585_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76796..77293)
                     /gene="sspB"
                     /locus_tag="OO585_000080"
     CDS             complement(76796..77293)
                     /gene="sspB"
                     /locus_tag="OO585_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6627802.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77299..77937)
                     /gene="sspA"
                     /locus_tag="OO585_000081"
     CDS             complement(77299..77937)
                     /gene="sspA"
                     /locus_tag="OO585_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6627803.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78332..78724)
                     /gene="rpsI"
                     /locus_tag="OO585_000082"
     CDS             complement(78332..78724)
                     /gene="rpsI"
                     /locus_tag="OO585_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6627804.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78740..79168)
                     /gene="rplM"
                     /locus_tag="OO585_000083"
     CDS             complement(78740..79168)
                     /gene="rplM"
                     /locus_tag="OO585_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6627805.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79386..80513)
                     /gene="zapE"
                     /locus_tag="OO585_000084"
     CDS             complement(79386..80513)
                     /gene="zapE"
                     /locus_tag="OO585_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6627806.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80707..81105
                     /locus_tag="OO585_000085"
     CDS             80707..81105
                     /locus_tag="OO585_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6627807.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81259..82626
                     /gene="degQ"
                     /locus_tag="OO585_000086"
     CDS             81259..82626
                     /gene="degQ"
                     /locus_tag="OO585_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6627808.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82716..83783
                     /gene="degS"
                     /locus_tag="OO585_000087"
     CDS             82716..83783
                     /gene="degS"
                     /locus_tag="OO585_000087"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6627809.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83846..84784)
                     /gene="mdh"
                     /locus_tag="OO585_000088"
     CDS             complement(83846..84784)
                     /gene="mdh"
                     /locus_tag="OO585_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6627810.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPSEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85219..85689
                     /gene="argR"
                     /locus_tag="OO585_000089"
     CDS             85219..85689
                     /gene="argR"
                     /locus_tag="OO585_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6627811.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86054..86317
                     /gene="yhcN"
                     /locus_tag="OO585_000090"
     CDS             86054..86317
                     /gene="yhcN"
                     /locus_tag="OO585_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6627812.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86372..86644)
                     /locus_tag="OO585_000091"
     CDS             complement(86372..86644)
                     /locus_tag="OO585_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6627813.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86736..88703)
                     /gene="aaeB"
                     /locus_tag="OO585_000092"
     CDS             complement(86736..88703)
                     /gene="aaeB"
                     /locus_tag="OO585_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6627814.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTERVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88709..89641)
                     /gene="aaeA"
                     /locus_tag="OO585_000093"
                     /pseudo
     CDS             complement(88709..89641)
                     /gene="aaeA"
                     /locus_tag="OO585_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            complement(89649..89852)
                     /locus_tag="OO585_000094"
     CDS             complement(89649..89852)
                     /locus_tag="OO585_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6627815.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
BASE COUNT        22130 a        24160 c        22360 g        21335 t
ORIGIN      
        1 atgacagcct ggatattaaa accacattac aggcaaacga cattccagga ctcacaaccg
       61 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
      121 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
      181 gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
      241 cgaaaacgga tattgccgtc attaaaggcg tgtgggtcga tggaatggga agcccgtcta
      301 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
      361 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
      421 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
      481 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
      541 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
      601 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg
      661 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
      721 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
      781 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
      841 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
      901 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
      961 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
     1021 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     1081 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     1141 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     1201 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     1261 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     1321 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     1381 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     1441 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     1501 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     1561 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc
     1621 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     1681 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     1741 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1801 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1861 ccactgctta tatgcagggc acagcgcagg ctgattctgc cttttatctt cagcagatgc
     1921 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1981 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     2041 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     2101 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     2161 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     2221 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag cagaatattg
     2281 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     2341 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     2401 gtaacgatcc cgatatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     2461 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     2521 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     2581 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     2641 tagctgccgc agacgtagca gaacaacctc agccgcaaac tgcggatggc gttgccagcc
     2701 cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag ccggtgcctg
     2761 taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc
     2821 tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag gttcagcagg
     2881 atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga
     2941 gcaacacccc gctgaacgtg ctggtactga accagccgga gaatatcgaa aaccgcgtca
     3001 atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc
     3061 gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg
     3121 tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa
     3181 aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa
     3241 cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa
     3301 cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg
     3361 tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta
     3421 acggtagcca gagcggtgca atgctgtacg ccagctcccg cagcgcacaa ggcaccgcag
     3481 gcccggattt ccgtctggag atggaaggtt tgcagtacag cgaaatcccg atgctggcgg
     3541 gcggtaatct gccgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg
     3601 ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc
     3661 gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca aacccggatt
     3721 gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca
     3781 gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg
     3841 caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggtgaga tcgatctgat
     3901 aatgcgtgaa ggctggacca ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta
     3961 tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg
     4021 cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt
     4081 agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt
     4141 aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa
     4201 ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg
     4261 ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca
     4321 atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac
     4381 ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac
     4441 atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag
     4501 ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc gccgttacgc
     4561 gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca ggtttgttag
     4621 ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc
     4681 atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg
     4741 atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc
     4801 tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg
     4861 caactgaccc acgcagtgtc ggtacccagg tggacgatgg taccctggaa gtgcgcgtga
     4921 acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat gtcacggcgt
     4981 atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca
     5041 aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc
     5101 agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa gtgcgttcgc
     5161 agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg
     5221 aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca
     5281 gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa tagcagcccc
     5341 ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta
     5401 ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
     5461 ccagcccggc caccagacaa atcgccgcaa aaccccagcc gaggacaaac cccataaaca
     5521 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
     5581 atacctgttg gcgacgcatc cccgccgcga ccggagccgc acagcaggta cacatcatgc
     5641 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     5701 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
     5761 ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
     5821 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa
     5881 acgctttgcc gtagtaatgt tgccatttca cataccagag accggcaatg acaacgagaa
     5941 agaaaagcgc gggtttccac cactgaatgg gtgttgccgc ctgagatgaa gactgaccag
     6001 tcatagcatt ccctaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     6061 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     6121 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     6181 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     6241 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     6301 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     6361 agtgggcatt ggcccccata gcactgacca ccagcatatg ctgcgcgccc agccgccgcc
     6421 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
     6481 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt tcggtgacct
     6541 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
     6601 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca
     6661 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
     6721 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccagtcttaa tagaccatga
     6781 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     6841 tgagtaagaa aattgccgtt ttaatcagtg atgaatttga ggattcagaa tttacttcac
     6901 ccgcagacga gttccgtaaa gccggacacg aagtaattac cattgaaaag caagcgggta
     6961 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
     7021 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     7081 gtggggacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
     7141 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     7201 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
     7261 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
     7321 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
     7381 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
     7441 ggggccgaca gcattctggc aaccggaaag cgatgattgt acgccttgcg cgcgaggtcg
     7501 ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
     7561 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
     7621 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
     7681 taaaaagcat tagcgcacca cagttcccct tcctgctgca cacaccaggt gacaacatgt
     7741 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
     7801 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
     7861 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
     7921 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
     7981 ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
     8041 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
     8101 cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg
     8161 gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
     8221 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
     8281 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
     8341 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
     8401 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
     8461 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
     8521 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
     8581 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
     8641 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
     8701 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
     8761 catgcagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
     8821 ctggatcttg tttacgcgcc gcaatcatca gcagatcgct gacgtcggca ctaaaactca
     8881 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
     8941 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
     9001 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
     9061 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
     9121 gggaacgaag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
     9181 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
     9241 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
     9301 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
     9361 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
     9421 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
     9481 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
     9541 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg
     9601 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gaatacgccg
     9661 ccgagcgtta tccgaatatt gagcgccacg tatcggtgca ggcttcggcg accaatgaag
     9721 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
     9781 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtaccactg gaagtgtttg
     9841 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
     9901 gtgagtcgcc taacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
     9961 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
    10021 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    10081 acgcgctgga agaaccaacc agtctcaata ccttggaact gctgccggag ttaatggcgg
    10141 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    10201 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa agcgcgtgga
    10261 tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg tatcaccgta
    10321 aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa
    10381 agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct
    10441 tggagaagcg gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc
    10501 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc
    10561 atcctctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc
    10621 cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca
    10681 cgcgctgaac tgctacaacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg
    10741 ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga
    10801 tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct
    10861 ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga
    10921 aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt
    10981 gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct
    11041 gacatccatg cagggattgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt
    11101 cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcgccactat tgttgacggc
    11161 aaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt
    11221 aaatatgctc gtcatacttc aagttgcatg tgctgcatct gcattcgttc accccagtca
    11281 cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat
    11341 ttagagcaca gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt
    11401 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ttactcgatc
    11461 tggcccccat tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg
    11521 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    11581 ctggcattgc cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca
    11641 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg
    11701 aacagttcgg cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg
    11761 cgccaggtag tgaccaacgg acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg
    11821 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    11881 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    11941 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact
    12001 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    12061 cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    12121 gcgatgccga attcctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgcggcg
    12181 aaaccgggca actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac
    12241 agtatggtgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc
    12301 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtc aacgggcaga
    12361 ttttcgacca ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt aaggaagagt
    12421 tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg
    12481 tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    12541 accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg taccaatgcg
    12601 ttgccgacgc caaacaccag aatcagcgta atcatcggct tgccgcccca gacgcggaat
    12661 ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc
    12721 cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccatt cgggaacagc
    12781 agaccaccca caacaggcgg tgcaaaggtc aacaatgctg ttttcaagcg gcccatagcc
    12841 gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    12901 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg
    12961 ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctctgcaata
    13021 ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc
    13081 gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    13141 taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc
    13201 aatgtcatca gcagatacgg cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc
    13261 gcaggctgca catgccccaa caggctgcca aaggtgagga agaaggtaat gactttcgcc
    13321 cccagcacaa tcgccgtcat gcggctgacg gccttagtac tcaaccacac cacaaacgct
    13381 accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg
    13441 aaggtgtgat gcagaattga accactggca gaaatatagg cataggtcag gatatagagc
    13501 acaaaggcaa tggaaatacc gttgaccacg ttccagcctt tgcccagcaa atctttggtg
    13561 atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    13621 gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc
    13681 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    13741 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    13801 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacgcga
    13861 gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat attattttac
    13921 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    13981 gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    14041 ccacggccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    14101 ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    14161 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    14221 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttacca
    14281 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg acgaacttca
    14341 acaccatcat cacggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    14401 tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg atcgcgcgga
    14461 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggtacg
    14521 atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc
    14581 agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga
    14641 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc
    14701 agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg
    14761 ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc
    14821 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    14881 ttaactacca ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc
    14941 aggatgtcca gacgaccatc tttcaggcgt tccagtgtct gttcacgcag cgcctggttc
    15001 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc agccacttcc
    15061 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    15121 aaacgtacca gtgcttcgtt tttgcgcata ccccagacag tccagtagct ctggctgatg
    15181 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    15241 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    15301 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    15361 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    15421 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    15481 aattgcacgt cataacgctg gccgccgtac agagcaacca catttacgcc gcgcatgtgt
    15541 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    15601 accagaatct gtggtgcttt cagctcagga tcaagattct gcaacagagg taaagagaat
    15661 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    15721 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    15781 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    15841 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    15901 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    15961 tcacccctta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    16021 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    16081 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    16141 tggcgctgtc cgaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    16201 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    16261 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    16321 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    16381 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    16441 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    16501 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    16561 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaacact tcaggcatat
    16621 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    16681 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    16741 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    16801 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    16861 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    16921 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    16981 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    17041 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    17101 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    17161 ctttctcaac gcgtttgtca gcgatttgag agatgtggac cagaccttct ttaccgccgc
    17221 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc
    17281 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    17341 cgtcggtcgc tgcgatcttc acagtaccgt catcttcgat ttcgatggta gtgccagttt
    17401 cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg
    17461 ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt
    17521 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    17581 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    17641 taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    17701 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    17761 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    17821 aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg
    17881 ggaatttgtc catatccggc atgactgcca gcacgctgcg cttcgccaga cgaccgtgac
    17941 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    18001 agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    18061 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    18121 gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat
    18181 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt
    18241 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    18301 gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    18361 cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca
    18421 gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca
    18481 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    18541 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagaaca gcggcttcag
    18601 taccggcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc gggttcagta
    18661 cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac
    18721 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg
    18781 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    18841 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttggacgac
    18901 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    18961 taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa
    19021 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    19081 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    19141 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    19201 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    19261 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    19321 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    19381 ctcggcagga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    19441 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    19501 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    19561 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    19621 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    19681 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    19741 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacttgt tatcgcaaga
    19801 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcgacgc ggccttcatc
    19861 gtcaatttcg cccataccga taaacttgcc gttctcacct tctgtaacgc gaaccagtcc
    19921 ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt
    19981 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    20041 atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    20101 atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    20161 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
    20221 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
    20281 caattcataa atggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
    20341 ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    20401 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg
    20461 ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    20521 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgaa
    20581 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    20641 ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt
    20701 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    20761 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    20821 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    20881 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    20941 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    21001 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    21061 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    21121 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    21181 actgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata
    21241 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg
    21301 catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc
    21361 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    21421 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    21481 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    21541 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    21601 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    21661 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tctaggcttt ccgcttcaat
    21721 cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc
    21781 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    21841 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg
    21901 tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa
    21961 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    22021 acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    22081 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    22141 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    22201 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    22261 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    22321 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga tgccggtacc
    22381 cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttctg gcaggatgcc
    22441 atactgggag agttcgttct taacacgatc cggatcggct tctggtttat caatcttgtt
    22501 cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg
    22561 cggcatcacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    22621 acgagcacgc attgaagtaa acgcggcgtg ccctggggtg tccaggaagg tgatcatgcc
    22681 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    22741 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    22801 catgatggtc acaaccggcg cgcgcggttc agccgcagcc cccgtgtcac ggtcgctcat
    22861 taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc
    22921 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    22981 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    23041 ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagctggct tctggaagcc
    23101 ttgctgcagg gaagaacctt tacgttttcc gcctttacct ccacgtactg ctgcgcgagc
    23161 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    23221 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    23281 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    23341 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    23401 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    23461 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    23521 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc
    23581 tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg
    23641 acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gcttcacgct gcgcttgctc
    23701 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    23761 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    23821 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    23881 caaagtctgt ttctcttgtg cggacacaga gtcgtcagca gacttccgga tacctgcatc
    23941 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    24001 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    24061 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca ccccttcgat
    24121 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    24181 ggccagtttg aatgccaaat cacgatctat cccttcaagg ttcagcagat cgtcagccgg
    24241 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    24301 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    24361 catcggcaca taggccagtt cttccagcgt cgagaagcct tcttctacca gaacagtcgc
    24421 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    24481 ctgatgctta gcctgcaggt cgtcaacggt catcacgttg agttcccaac cgctcagctg
    24541 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccggcttc
    24601 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    24661 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    24721 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacacaggc
    24781 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    24841 atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca cttcaatacg
    24901 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    24961 ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    25021 cggcagcata tcttcgcgca ggatcacggc ttcagcattg ttgcccagct ccagagagat
    25081 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    25141 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    25201 cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    25261 caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    25321 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    25381 gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg tcgccagcgc
    25441 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    25501 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    25561 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    25621 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    25681 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    25741 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    25801 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    25861 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgtgaccgcg
    25921 aataaattcg atgccaacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    25981 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    26041 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    26101 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    26161 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    26221 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    26281 ccaccccgcg cctgaaactc ggcaaattct actcgttttg ggtaaaagat gcaaatactg
    26341 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    26401 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    26461 tgaatacttt tgccttctcc tgctctccct taagcgcatt attttacaaa aaacacacta
    26521 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    26581 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    26641 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttctggcggt
    26701 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    26761 actgcaaacc taggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    26821 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    26881 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    26941 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    27001 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    27061 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    27121 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    27181 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    27241 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    27301 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    27361 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcaacgacgt agaaatgatg
    27421 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    27481 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    27541 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    27601 gcgcatggtc gtcagttggg ccgtctactg taccaggggc gttggtttga ctcccaggcg
    27661 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    27721 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    27781 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    27841 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    27901 tatgccaaaa ctggcctgct ctcctcctct gccacttcag gcgtgccgca ggtggagaat
    27961 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcagggggg ggttaccttg
    28021 cttatcctct gtaaactcct ttctgaacat cacataatcg aaaacatcat ggtttgcatc
    28081 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    28141 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    28201 gttgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    28261 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    28321 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    28381 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    28441 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    28501 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    28561 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    28621 catggcgcgc atggcgatac tggtaactgc agtaccattc tgccgaaaag cggattgcga
    28681 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    28741 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    28801 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    28861 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    28921 ctcccccaca atcaacacgt aggtatcaat acctgtatcc ctgactgata attgaaaata
    28981 cggcaccgtg tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata
    29041 gttgagattg aaaaacggcg tataggtagc aaatcgcgac gctagtatat atggactgaa
    29101 cgttttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    29161 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    29221 tattattact gcccaaaaaa gaagggataa aaaagcaaag gcacatagat aagggatata
    29281 catccccagc attttgacaa cttcatccgg atcactctgg agcacactaa tcgcaaatcc
    29341 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    29401 agtcagtaac acaaatggtg cggcaacaag cagacgaagc ggaagggatg atatacgttt
    29461 tacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg cgccggaaga
    29521 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    29581 aaaacttcta gcaaatttgt tgaatactgt catccagaaa tccaagttca caaccaatat
    29641 aaacaattat caacaatgtt caattttata tcagacatgt ttttcaactc tgatccatat
    29701 cagaccgaaa cactgttagt tttttctttt taaatgatgc ggccattgcg ttctcagtaa
    29761 ttaaataaca ttatcaatgc gtctctaaac attacgcaga acgtagccat cagtagttat
    29821 actgacagga gcaggatggt ttatcgagaa gtaaacatat ccgtattcgt accggaactg
    29881 gagttgacac aataaagtgc caattatgtc agcagaaggg aaaagtttat aactaaggcg
    29941 taccggcacc atcgtttcaa ggtaccagct acgagtaaag caactggacg agatacagat
    30001 acctgacaac cattcctcag accaggacca aaacgaaaaa agacgctttt cagcgtctct
    30061 tttctggagt ttggtaccga ggacgggact tgaacccgta agccctattg ggcactacca
    30121 cctcaaggta gcgtgtctac caattccacc acctcggcac ggatactact ttttagttcg
    30181 ggatatcgct ggtcggctta gccggagcag ctggttgagt ttgttcggtt ttcgccggtg
    30241 cactcagatt ttcccattcg ctacctttat tggttttgtt gctgttgatg ttacccagca
    30301 ccagactgat gatgaagaat aacgttgcca gcagcgccgt catgcgggtc atgaagttac
    30361 cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc tccgaaggag gctcccatat
    30421 cagcgccttt accttgctgc agcatgatca gaccaacaag gccaattgcc acaataagga
    30481 aaactaccaa aagagcttcg tacataatca acctgttcct tgcggagttg ccgcgtacca
    30541 atgcttcaac caataaagcg ggagttttta atctttccca ctgaagcggg tgtgaatact
    30601 aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca ttgtatcaac tgcggaaaaa
    30661 aacagcaaaa ccgatgtatt cgctgagaaa aaaggcgaca atcgccgcct ttttagccag
    30721 tcatttatcg ttttaaacgg cttttactgc atcggcgatg cggtgtgcaa attcagtcac
    30781 ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac ggttcggtgc cggatttacg
    30841 cagcaacacg cgtccacggt tgcccagcgc agcttcaacc tctgcggtca cggctttaac
    30901 tgactcatgc tcaagcggat cgccgctacc tgcggtgtaa cgcacgttaa ccagaatctg
    30961 cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc atatggttac gtgccatcgc
    31021 cgccagcacc tgcaagccag caacgatgcc gtcaccggta gtggttttat ccagcaggat
    31081 cacatgaccg gaattctctg caccgatacg ccagcctttc tcctgcattt tttccagtac
    31141 gtagcggtca cccactttcg cgcgcgcaaa tggaatacct aactgtttca gcgccagctc
    31201 aagccccatg ttactcatca atgtacccac agcaccacca cgcagctggc cctgacgaag
    31261 accttcacgc gcgatgatat acatgatctg atcaccatcg actttattgc cttcatggtc
    31321 aaccataatc acgcgatcgc cgtcgccgtc gaaggcaata ccgagatccg ctttttcagc
    31381 cagcacacga gcctggagcg cgcgaacgtc ggtagccccc acttcggcat tgatgtttac
    31441 accgttcggc tcacaaccga tagcgataac gttcgccccc agttcacgca gcacgttcgg
    31501 cgcgatgtga taagtcgcac cgtttgcgca atccaccaca atcttcagtt cgctgaggct
    31561 aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag cgacccgcag catcaacgat
    31621 acggctggct ttacccagtt ctgccgaatc aacacagctg atctcttttt ccatttccgc
    31681 ttcgatggcc tcttctaccg catccggtag tttggtgccg tcgatagaga aaaatttaat
    31741 gccattatcg tagaacgggt tatgcgatgc agatatcaca attccggcct ctgcgcggaa
    31801 ggtacgcgtc agataagcca cggccggtgt tggcatcggg ccagtgaaga gtgcggaaag
    31861 gcccgctgcc gccagacccg cttccagtgc tgactccagc atatagccag aaatacgcgt
    31921 gtctttacca ataatgatct tacgggagcc gtggcgcgcc agcactttac ccgcggccca
    31981 acccagctta agcacaaaat caggtgtgat cggcgcatcc cctacacgac cacgaatccc
    32041 atcggtaccg aaatatttac gattactcat agcgtttgtt ttcctttgca gacagagtgg
    32101 cttccaccac ccgcatcgct tctacggttt ctttgacgtc atgaacacga ataatgtgcg
    32161 cgccttgcat tgcggcaatg accgcacagg ccagactacc gctcaggcgc tcggacggcc
    32221 ccacgttcag cagctgccca atcatcgatt ttcgtgacat acctaccaac agcggcaggt
    32281 tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata gttatgggag agatttttac
    32341 cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc gatacccgcc tgctcgcaac
    32401 gtgctatttg ctcaataaag tagcgattca cttctgcaaa gacatcgtca tacttcggag
    32461 cttcctgcat ggtttttgga tttccctgca tatgcatcag acaaaccggt aaaccggttt
    32521 ctgccgccgc ctccagagcg ccaggttcgg aaagggagcg gatatcgtta ataatgtgag
    32581 cgccaacttt cgctgactca cggatgactt ctggtttgga tgtatctacc gagagccaga
    32641 cttcgaagcg ttgagcaatt gcctcaacca caggaataac acgttgcaac tcttcttcaa
    32701 cgctaacttc cgccgcccct gggcgcgtgg actcgccacc aacgtcaatg atcgtcgcgc
    32761 cagcgttgat catcagattc gcatgtttca ccgcatctat cagcgagtta tgcgtgccac
    32821 catccgaaaa ggaatcaggc gtgacgttga ggatccccat tacgtgagga tggctaaggt
    32881 ccagtgaagt gccctgggca aagagtttca tggtgttatc cctggtatga attgataaga
    32941 aaaaaccccg gagcacgccc cggggttttc ggtacaaata cagtcatctg atgcgggaac
    33001 ttacttgtcg cctaactgct ctgacatggt gttacccggg ttcggcgtac gcggttcatc
    33061 aaccggacga ggagcctttg gactaccatt gtcgccagaa ttgttagaag cgcctggttc
    33121 ttcccagccc gctggcggac gtacatcgcg acgtgccatc aggtcatcaa tctgcggtgc
    33181 gtcgatagtc tcatatttca tgagagcatc tttcatcgca tgcagaatat ccatattgtc
    33241 ggtcagaagc tgacgcgcac gattatagtt acgctcaatc agtgctttca cttcctggtc
    33301 gatgatacgt gcagtttcat cggacatatg tttcgctttc gctacgctac ggccgaggaa
    33361 cacttcacct tcttcttccg cgtacagcag tggacccaat ttctcagaga agccccactg
    33421 agtcaccata ttacgcgcca ggttggtcgc aactttaata tcgttggacg caccggtaga
    33481 aacatgttcc ggcccgtaga tgatctcttc tgccagacga ccaccgtaca gcgtagaaat
    33541 ctggctttcc agtttctgac ggctggcgct gattgcgtcg ccctcaggca agaagaaagt
    33601 cacacccagc gcacgaccgc gtgggataat cgtcactttg tgcaccggat cgtgttccgg
    33661 caccaagcga ccgataatcg catgacccgc ttcgtggtaa gccgtcgatt ctttctgcgc
    33721 ttccgtcatc accatggagc gacgttccgc acccatcatg attttatctt tcgctttttc
    33781 gaactcaatc atcgacacaa cgcgtttgtt gccacgagca gcgaacagtg ccgcttcgtt
    33841 taccaggttc gccaggtcag caccggagaa accaggagta ccacgggcaa tgattgccgc
    33901 gtcgatatcg ggtgccaatg gtacgcgacg catgtgaact ttcaggatct gctcacgacc
    33961 gcgaacatct ggcaagccaa ccacaacctg acggtcgaaa cggccaggac gcagcagggc
    34021 tgggtcaaga acgtccggac ggttagtcgc ggcgataacg atgatacctt cgttaccttc
    34081 gaagccatcc atctcaacca gcatctggtt cagagtctgt tcacgttcat cgtgaccacc
    34141 gcccagacca gcgccacgct ggcggcctac ggcgtcgatt tcatcgataa agatgatgca
    34201 cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca cgggatgcac ccacaccgac
    34261 gaacatttct acgaagtcag aaccggagat agtaaagaac ggaactttcg cttcgcctgc
    34321 aatcgctttc gccagcaacg ttttaccggt acccggagga ccgaccatca ggacgccttt
    34381 cgggatctta ccgccgagtt tctggaagcg gctcggctcg cgcagatact ctaccagttc
    34441 agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca gcaaaggtcg ttttgatctg
    34501 atcttccgtc agcatgcgcg ctttgctctt accaaacgac atggcacctt tgccaccgcc
    34561 gccctgcatt tgacgcatga agaagatcca gacaccaatc agcaacagca tcgggaacca
    34621 agagatgaag atagaagcca gcaggcttgg ttcttcaggc ggttcaccga caaccttgac
    34681 gttcttggtc aacaggttat ccagtaattt cggatcctga accggaatgt aagtggtata
    34741 acggttacta tctttcttgg taacgttgat ttcacgtccg ttgatacgcg cttcacgaac
    34801 ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc accttacggc cattagactc
    34861 gctgggccca aagctctgga atactgacat cagcacaacg gcaatgacca gccagagtat
    34921 taggtttttc gccatgtcac tcaagggatt aacctcttat tacaactgtg ttaaaaacag
    34981 cgtcaggata ctctatatcc agcatctttc aaactttcgt ctgaaatctc ccggttaggg
    35041 tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt gcacgagaag agtccggctt
    35101 acgaactttg accttcgtaa acagggagcg aatttccctt agatactcat cgaaaccttc
    35161 gccctggaac accttcacta caaaactgcc acctggcgct aatacatcac gacacatttc
    35221 taacgccagt tccaccagat acatggcacg ggggatatcc accgccggtg ttccgctcat
    35281 gtttggtgcc atatcggaca tgacaacctg gactttgctg tcgccaacgc gctccagcag
    35341 tgctttcatc accagttcat cacgaaaatc gccctgaaga aagtccacac caacgatagg
    35401 atccataggt aaaagatcgc aagcgatgat gcggcctttg ccgccaattt gggtgaccac
    35461 atattgtgac caaccacccg gagcagcacc aaggtcgaca accgtcattc ccggtttaaa
    35521 gagtttgtca ctttgctgta tttcatcaag tttaaaccag gcacgggaac gtaacccctt
    35581 tttctgtgcc tgttgaacat atttatcgct aaagtgttcc tgaagccagc ggctggagct
    35641 ggcagaacgc ttcttacctg tcatttaact ttcccatggg gataactcat cgtaaccaat
    35701 tgcgtaaatt tttacgcgcc tatttggtga tatatgggag atggcggtag aatgacccgt
    35761 tttcaatccc aacgtaagca aaaaatacga tgaatctgag tactaaacaa aaacagcacc
    35821 tgaaaggtct ggcacatccg ctcaagccag ttgttctgct tggcagtaat ggtttgaccg
    35881 aaggggtgct ggccgagatt gaacaagcgt tagagcacca tgaactcatc aaggtgaaaa
    35941 tcgccaccga agatcgcgaa acaaaaacct tgatcgtgga agctatcgtg cgcgaaaccg
    36001 gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct ttatcgccca actaaagaac
    36061 gtaaaatctc gctgccacgc taagattatc ctaaagttac acacatttgc tgtgtaaaac
    36121 gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc tgttcgttga taaaaggccg
    36181 catagcggcc tttttccttt ctttacaata catcaacatc ttgatgtatt gggtaattct
    36241 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    36301 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    36361 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    36421 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    36481 ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt aatacggcct
    36541 tcgcagaaac cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    36601 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    36661 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    36721 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    36781 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    36841 ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg
    36901 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    36961 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    37021 gccgcgaatg ttgatgagta cgttactcaa ctccccgctg gtgccaacct tgccctgatg
    37081 gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    37141 cccgccagta cgcagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    37201 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    37261 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    37321 atcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    37381 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    37441 tttagcgctc cgcctgccgc tgccatagtt gaccgcaact gtttctccgt ctcgctctac
    37501 agtgccccaa aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    37561 atgttcagcc aggtacgcac cctcccccga ggttctgccg aagcgcaata ctgcgaactg
    37621 gatgtcatac caggcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    37681 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg ggcaattctg
    37741 aaagatgagt taaaacaggc aggtatcacc tggagcggaa cactgctgcg ccagactcag
    37801 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgttgca cgatctgctt
    37861 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgataggc
    37921 catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt gcgtcagatc
    37981 ctgcgccagc aagccggtgt cgatattgga aacaccatta ttgccgatgg ttcagggctt
    38041 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgttgcaata cattgcccaa
    38101 cacgacaatg aacttaactt tatctccatg ctgccgctgg cgggctatga tggctctttg
    38161 cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt
    38221 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    38281 gcgtttgtac aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt
    38341 attccgttag tgcgttttga aagccgtttg tataaagata tttatcagaa caattagtca
    38401 aaaagaaacc ccggcacatg gccggggttt tgctgattaa cgcttgtaaa tgaactcaac
    38461 gccttcttcg tcgtcttcgt cccagtcatc atcccagtcc tcatcatcct cttcagcaat
    38521 ctcttcaagc tgctggcgat gataatcatc ccacatgaat tcgactttct ctggctgttt
    38581 cgcttcttca gcctgcacga ccgggttttc aatgataaag gtcatcacat cccagcagag
    38641 atctttcacg cccagtccac tcgccgcaga gatcagataa tatttatctt cccagcccag
    38701 cgcttcagca atcgctttcg ctttctcttc agcttctgcc ttatccagca gatcgatctt
    38761 attgaacact aaccaacgcg gtttcgccgc cagatcctgg ctgtattttt ccagctcgct
    38821 gataataata cgcgcgtttt caaccggatc ggtgccgtca atcggatcga tatcgatgag
    38881 gtgcaacagg acgcggcaac gttccaggtg cttcaggaag cggataccca gacctgcgcc
    38941 ttccgcagcg ccttcaatca gtcctggaat atcggcaaca acgaagctct tttcgttgtc
    39001 cattcgtacc acacccagac ttggcaccag agtggtaaac ggataatccg ccactttcgg
    39061 tttagccgcc gataccgcgc gaataaaggt cgatttaccc gcgtttggca tccccaacat
    39121 accgacgtca gccagcagca tcagctccag cagcagctca cgcttatcac ccggcgtacc
    39181 gttggttttc tgccgtggtg tacggttaac ggacgatttg aaacgggtat tgcccagacc
    39241 gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt ttagtcatat cgcccatggt
    39301 ttcaccagta ccctggtcga ttacacgcgt acctaccggc actttaatcg tcacgtcttt
    39361 accgcgctta ccggtacaat cgcggcttgc gccgttctga ccgcgctctg cacggaaaga
    39421 tttttcaaaa cgataatcga taagcgtgtt caggttttcg tcggcttcca tccatacgtc
    39481 accaccatca ccaccgtcgc cgccatccgg gccgcctttc ggaatatact tttcgcggcg
    39541 gaagctcacg caaccattac cgccatcacc tgcaacgacc agaatcgatg cttcatcaac
    39601 aaacttcatt ttattctccg taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt
    39661 atgcttacgt aatccgcccc aaatacgatg accaatggcg gaatacatcg cgcccgcaac
    39721 cacgacaaac gcaccgagat aacctaaaag gtttaacatc ggtctggcga agaaatcggg
    39781 ccaggccagt gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat
    39841 cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc gccagggcgc catatcctac
    39901 caatgtattc agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa gctgcgctat
    39961 cactcctggc tttgccagag ggaagagcgc aattgtacat aaagtgtaca gcaaaaacag
    40021 gatctgcggt gaggccagcc gacgcaataa aaccttttgc gccacgccat aactcaccca
    40081 aaccgtcgcc gcaccgaccc caaagatgac tccccaggtg taatcagtga gctttgtaaa
    40141 tatctcgacc agactggtgt taaaaaacat caccaggccg ctcaggagca tcaatgcccc
    40201 tacaacctga gtgctgcgca ttttctcttt caggataaat acgctggcaa ccatcatgcc
    40261 aactggcgag agttgcccaa tcacctgcga agcggtcgga ctcaggtatt gcaaggatga
    40321 gctgaacagg atgaagttcc caaacagccc ggcggtcgcc actgccaaca aaatcaacca
    40381 gcgtggctta cgaaacacgc gtaatggcgg caacctcttc ttcaccgcaa gaatggcacc
    40441 caggccaata ctcgccatca agaaacggta aaacacgatt gtcggaggtt ccatcacctc
    40501 cagcacctgc ttcattgcga ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa
    40561 aagaatgcca atgcctgcct gctgcttcat accgtattcc ctgcaaagcg catttgcgga
    40621 ttaacgtcgc accatgctca tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt
    40681 tgcggggctt tcatccgtta ccgggacgcg aaaaacttat tcagcttcga tgctgataaa
    40741 tttacggttt ttcgggcctt taacttcgaa tttcacttta ccgtctgctt tagcaaacag
    40801 agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg aatttggtac cacgttgacg
    40861 aacgatgatg ctacccgcca gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt
    40921 agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa
    40981 tctctcctca ggtcttaggc gctgatgcca gtaattttca catcagtgaa ccactgacga
    41041 tggccctgct gcttacgata gtgtttacga cgacgaaact taacgatttt aactttctcg
    41101 ccacgaccgt gagcaacaac ttcagctttg attacgccgc catcaacgaa aggaacgccg
    41161 attttgactt cttcaccgtt tgcgatcatc agcacttcag cgaactcaac agtttcgcca
    41221 gttgcgatgt ccagcttttc caggcgaacg gtctgacctt cgcttactcg gtgttgttta
    41281 ccaccacttt ggaaaaccgc gtacatataa aactccgctt ccgcgcacac cttttcaatg
    41341 attcagagtg cgctataaat attcacaata gggcgcgaat attacgcaaa acgcacgcct
    41401 ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg gtaacgttta
    41461 tctgtgccat tttttcagta caatcaccct atattcctaa ccataaaccc taagttgcct
    41521 ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa
    41581 tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt gagcagctta
    41641 attccgacgt ccaactgatc aatcagttag gctattacat cgtcagcggc ggcggtaaac
    41701 gtattcgtcc gatgattgct gtactggctg cacgagctgt tggctatgag ggaaatgcgc
    41761 atgtcaccat tgctgccctg atcgagttta tccacacggc gactctgcta cacgacgacg
    41821 ttgtggatga atcagatatg cgcaggggta aagctaccgc caacgctgca tttggcaatg
    41881 ccgccagcgt gctggtaggc gattttattt atacccgcgc gttccagatg atgaccagcc
    41941 tcggttcact caaagtgctg gaagtcatgt cagaagctgt aaacgtcatc gcagaaggtg
    42001 aagttctgca actgatgaac gttaacgatc cggacatcac tgaagaaaac tacatgcgcg
    42061 ttatctatag caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc gggattctgg
    42121 ctggctgtac gccggaggag gagaaaggcc tgcaggatta tgggcgctat ctcggcactg
    42181 ctttccagtt gatcgacgat ttactcgatt acaatgccga tggcgaacag ttaggtaaaa
    42241 acgtcggcga cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc
    42301 atggcacacc agaacaggca cagatgatcc gtaccgccat cgaacagggt aacggtcgcc
    42361 atcttctgga accggttctg gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc
    42421 agcgcgccga ggaagaagca gacaaagcca tcgcagcgtt acaggtgctc ccggacaccc
    42481 cttggcgaga agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc
    42541 ctcccttcat cccgcgcagt gcgcgggatg attccagtaa attccataaa aacactaatt
    42601 cagcgctata acacctgaaa aaaagggcga tgaatcttta tatattctga atattcacac
    42661 tctttacagg aactttttag agcaataggc catcaggagt atagtgatgc ccgacagaag
    42721 aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa gtaatttcat
    42781 tgactggcat ccccctgaca tcattgcagg tttgcgcaag aaaggaacgt caatggcggc
    42841 ggaatctcgc agaaatggtt tgagttcctc aacgctggca aacgcattat cgcgcccatg
    42901 gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctggg ttatctggcc
    42961 atcacgctac catgatccgc agacccatga gtttatcgac agaacgcagt tgatgcgtag
    43021 ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg gctgccagct
    43081 ctcagacgat tactcgcctt tcacacgctt aatatttgca cctaaagcgc gcagtttgtc
    43141 ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc
    43201 cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg ccataacctg
    43261 tgcaccagaa agtttttcaa caccatgaca aataacggta ttgctttcga tttcggcatg
    43321 cgcgcccata cggctaagct ccggcacatg cataaagcgg ttttcaaaga ccgtttcggt
    43381 gatgaatccg gtcccttctg ccaccaggtt caacagcgtg aactgggcct gcatatcggt
    43441 cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt tgccatgcat
    43501 atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcgtcac gcagtttcgc
    43561 cagcacggcg tcgagagtat ctggctgcgc gttacggcag ataattttgc cgcgagaaat
    43621 cgccgccgcc accaggaaag taccggtttc gatacgatcc ggtagcacgc gatagacacc
    43681 gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac cgctaatttt
    43741 cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac gcgctgcgtt
    43801 ttcaataatc gtggtacctt ccgccagggt tgcagcacac atgatggtca ccgttgcgcc
    43861 aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga cggaagcttt
    43921 aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag
    43981 atcaaccgga cgcgcaccga tcgtacaacc gccaggcagt gaaacttgcc cctgaccaaa
    44041 gcgcgctacc agcggcccca gtgcccagat agaagcacgc atggttttaa ccagatcgta
    44101 aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat tacgttctac
    44161 tttcgcaccc agctgactta gcagcttcat tgatgtatcg acgtctttca gtttcgggac
    44221 gttctggatc tctaccggtt cttccgccag tagagcggca aaaaggatag gcagagcagc
    44281 atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct gaacacgaaa
    44341 tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg aatcgcccat
    44401 agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat acgctttgat
    44461 cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat agaccgtctg
    44521 ctgtttttta acccgactca tgccgtcaaa caactcaccc acggcaataa cctgaaagtg
    44581 gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca acacgctctg
    44641 aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg aaacatctta
    44701 gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca gacaaggctc
    44761 gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg aaaaaattta
    44821 acgaggcaga acatcagcag gcaaattata caatttcgcc agggtataca ctttgtcgtt
    44881 taccccctga agcgtcacat tgttgccctg ctttttcgcc agatcgataa gatggagcag
    44941 cagtgccagt ccccccgtat ccacgcggga cacacggcta agatcgatgc aggtaatccc
    45001 cttcaccgct tcctcacgca tttcccaaag cggtagcaaa acgtcctgat ccagctctcc
    45061 ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca ttattttttc
    45121 tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata
    45181 cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat actgacgcct
    45241 tcagcaatca tgtcgtaagc ctgccagttg cccgtctggg agtttttacg ccactggaag
    45301 tccagacgca ccggcgggcg gccattcgga tcaataatgg taacgcgaat cggcacaatg
    45361 gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccatg atacatcgcc
    45421 agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta ggcttcacgt
    45481 tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc gtatttcacc
    45541 tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg gttggcccga
    45601 atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc gtccatcagc
    45661 ttataaggat tggtctggtc tgccgcggtt gccgcactca gcggtgcaat caccagcaaa
    45721 gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt cgttgtaccc
    45781 acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc actattctta
    45841 ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag caccatcgca
    45901 gacttagtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag ttccgggtct
    45961 tcaaaaccga cgtttaatgc cagatattgt tcccccagta ggccggaagt acgaatgctc
    46021 agcgaactgg tatctggaat gtggttataa cgttgttcaa tttccagcgt tacgcgtggc
    46081 agataggttt tcgggtccag cgtaatatcc gccacccggc ccacaacaac gccaccaata
    46141 ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata aagcgtgtag
    46201 gtcggttcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa cagagccgcc
    46261 agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt ttgcatgtac
    46321 tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag ccagagacga
    46381 gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga tggcgtcgta
    46441 gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct taatcagaca
    46501 gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga agaacccgct
    46561 atcaatgcct ttccaactga cgccgaccag agatccgccc cagatcccca cggcgacgaa
    46621 aataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag aaataacccg
    46681 acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag cgcgcatcag
    46741 gccgatttct gcggttagcg ccgaaccagc acgcccggca aacaacaacg cggcaaccac
    46801 cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg tttccgcact
    46861 ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga acacgccaga
    46921 aaccacaata atcagcatcg acaggacgcc gacattatag agctggcgca ccagcagcgg
    46981 cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta acccggcccg
    47041 cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg catttaacag
    47101 catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca ggatagcgga
    47161 acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga tcaggattcg
    47221 cctgcaacgc ctgggcacag ccatgagcga caattttttt gtccgccagg atccaggcgt
    47281 gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa gtcacgccca
    47341 gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg ggatcttgcc
    47401 caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca cgcgccagcg
    47461 ctgcacgccg cgccatccca ccggaaagtt cagaaggcat tagtttagcc gctccacgca
    47521 gccccacggc ctccagcttc atcatcaccg tactatgcaa caatggcgcg ggaagttggg
    47581 tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg aacaacgccc
    47641 cggactgaaa taacatgctc atccgtttgc gcactgtata caggcgcgaa cgagacatcg
    47701 ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt tgcccgccaa
    47761 tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc
    47821 ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc gtaaaactga
    47881 catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct tcgtcttgcg
    47941 ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta cagaatatta
    48001 cccgctgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata aagtcaaaat
    48061 taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa
    48121 ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt ggtcgtttac
    48181 agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg cataccgccg
    48241 ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat catcgtctcg
    48301 cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct cggctcaaac
    48361 attatcaata ttttgctgat cctcggtctg gctgcgctgg ttcgtccttt taccgtccat
    48421 tccgatgttc tgcgtcgtga attaccctta atgttgttgg tcagcgtagt ggccggttcc
    48481 gtactctatg acggacaact tagtcgcagc gatggtatct ttctcctctt tctggctgtg
    48541 ctatggctgc tgttcattgt taaacttgca cgtcaggctg aacgtcaggg gactgacagc
    48601 ctgaccagag agcagcttgc agagctgccg cgtgacggcg gattgcccgt cgcgttttta
    48661 tggctcggca ttgcgcttat catcatgcca gtggccacgc ggatggtggt tgataacgcc
    48721 acggtgctgg cgaattactt tgccatcagc gagttgacga tgggtctgac ggcaattgct
    48781 atcggaacca gcctgccgga actggcaacc gcaatagcgg gggttcgcaa aggtgaaaac
    48841 gacattgctg tcggaaatat cattggcgca aacattttta atattgtcat cgtgttgggt
    48901 ttacccgcgc tgataacacc aggagagatt gatccattgg cgtacagtcg tgactacagc
    48961 gtgatgttgc tggtgagcat tatttttgcg ttgctgtgct ggcggcgctc cccgcaaccg
    49021 ggccgtggtg taggggtatt attaactggc ggatttatcg tatggctggc gatgttgtac
    49081 tggttatcgc caatactcgt tgaataactg gaaacgcatt atgtcgcacg tagagttaca
    49141 accgggtttt gactttcagc aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct
    49201 ggcggagctt gatcaataca tcaatcagaa tttcacgctt gcctgtgaaa agatgttctg
    49261 gtgtaaaggg aaagttgtcg tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat
    49321 ggccgcaacg tttgccagca ccggtacacc ttcatttttc gtccatcctg gtgaagccgc
    49381 gcatggtgat ttaggcatgg tcaccccaca ggatgtggtg attgctatct ctaactcagg
    49441 tgaatccagc gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg taccgttaat
    49501 ctgcatcacc ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt
    49561 taaagtagcg aaagaagcct gtccgttagg gctggcaccg accagcagca ccaccgccac
    49621 gctggttatg ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga
    49681 agattttgcg ctctcacacc caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa
    49741 cgatattatg catacgggcg atgagatccc gcatgttaag aaaacggcca gtctgcgtga
    49801 cgcattgctg gaagttaccc gcaaaaatct tggtatgact gtcatttgcg atgacaatat
    49861 gatgattgaa ggcatcttta ccgacggtga tttacgccgt gtcttcgata tgggagtgga
    49921 tgttcgtcag ttaagtattg ccgatgtgat gacgccgggg ggaatacgcg tgcgccctgg
    49981 cattctggcc gttgaggcac tgaacttaat gcagtcccgc catatcacct ccgtgatggt
    50041 tgccgatggc gaccatttac tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt
    50101 agtgtaaaga ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt
    50161 tacggccctg tcagcgccga cgttatagca aaagcagaga acattcgtct gctgatcctc
    50221 gacgttgatg gcgtactgtc agatggcctg atttatatgg gcaataacgg cgaagagctg
    50281 aaagcgttca atgttcgtga cggttatggc attcgttgtg cgctcacctc tgatattgaa
    50341 gtcgctatca ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc cacattgggg
    50401 atcactcact tgtatcaggg gcagtcaaac aaactgatcg cctttagcga actgctggaa
    50461 aaactggcga ttgccccgga aaatgtggct tatgtcggcg atgatctcat cgactggccg
    50521 gtaatggaaa aagtgggttt aagcgtcgcc gtggccgatg ctcatccact gttgatcccg
    50581 cgcgccgatt atgtgacgcg cattgctggc ggtcgtggcg cagtgcgaga agtttgcgac
    50641 ttattactcc tggcgcaggg caaactggat gaagccaaag ggcaatcgat atgagtaaag
    50701 ccagacgttg ggttaccatt gtgctatcac tggcggttct ggtgatgatc ggcattaata
    50761 tggccgaaaa agacgatacc gcccaggtgg tcgtcaacaa caatgatccc acctataaaa
    50821 gcgagcatac ggacacgctc gtctataacc cagaaggggc actaagctat cgattgattg
    50881 ctcaacacgt tgaatattat tccgatcagg ccgtttcgtg gtttacgcag ccggtactta
    50941 ccacgtttga taaggataaa atcccgacat ggtccgtaaa agcagataaa gccaagctga
    51001 ccaatgaccg gatgctctat ttatatggac acgttgaagt caacgcactt gtgccggact
    51061 ctcaacttcg cagaatcacg acggataacg cgcagatcaa tctggtgacg caggatgtta
    51121 cctctgaaga cctcgtcacg ttatacggaa caacatttaa ctccagcggt ctgaaaatgc
    51181 gcggcaactt acgcagcaag aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa
    51241 ttcaaaacaa acaaactcag ccttaatctt gtgcttgcca gctcacttct ggccgccagc
    51301 attccggcat ttgccgtaac cggagacact gatcagccga tccacattga atcggaccag
    51361 caatctcttg atatgcaagg caacgtggtt acctttaccg gtaatgtcat cgtcacccag
    51421 ggcaccatca aaattaatgc cgacaaagtg gtcgttaccc gtccgggcgg cgaacaaggt
    51481 aaagaagtga ttgacggcta cggtaaaccg gcaacgttct accagatgca ggacaacggt
    51541 aaacccgttg aaggtcatgc ttcccagatg cactacgaac tggcaaaaga ttttgtcgtt
    51601 ctgacgggta atgcttatct gcagcaggtc gatagcaaca ttaagggcga taagatcact
    51661 tacctggtga aagagcagaa aatgcaggct ttcagcgaca aaggcaagcg cgtaacaacc
    51721 gttctggtgc cgtcgcagct gcaggacaaa aacaacaaag gccagacccc ggcacagaag
    51781 aagggtaatt aattcgttat ggcaacatta actgcaaaga accttgcaaa agcctataaa
    51841 ggccgtcgcg tggtagaaga cgtcagcctg accgtcaact ccggggaaat tgtcggtctg
    51901 ctggggccaa acggtgccgg taagaccacc actttctata tggttgttgg cattgtgccg
    51961 cgcgatgcgg gcaacatcat tattgatgat gacgatatca gtctgctgcc tctgcatgca
    52021 cgcgcgcgcc gcggtatcgg ctatctgcca caggaagcct ccattttccg tcgcctcagc
    52081 gtttacgata acctgatggc ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt
    52141 gaagaccgcg cgaacgagct gatggaagag tttcacattg agcacctgcg tgacagcatg
    52201 gggcagtcac tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgcgc actggctgcg
    52261 aatccgaaat ttattctact cgacgaaccg tttgccgggg ttgacccgat ctcggttatc
    52321 gacattaaac gcatcattga acacctgcgc gacagcggcc tgggcgtact gatcactgac
    52381 cacaacgtgc gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat
    52441 ttgattgccc acggcacgcc tacagaaatc ttacaagacg aacacgttaa gcgtgtatac
    52501 cttggggaag acttcagact ctgatagggt agaagtttgc gacgttttag caggagagta
    52561 cgattctgaa catgaagcaa ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc
    52621 cacagcttca acaggcgatt cgtctgttgc agttgtcgac gctggaactt cagcaggagc
    52681 tacagcaggc gctggagagt aatccgctgc ttgagcaaat cgacactcat gaagaaatcg
    52741 acaccagcga aacgcaagac agtgaaacgc tggacaccgc cgacgcgctc gaacaaaaag
    52801 agatgccgga agagctgccg ctcgatgcca gttgggacac catttacacc gctggtacac
    52861 catccggcac cagcggtgac tacattgacg atgagctgcc ggtctatcag ggcgaaacga
    52921 cgcagacctt gcaggattac ctgatgtggc aggtcgagct gacaccgttt tccgacactg
    52981 accgcgctat tgctacctct atcgtcgatg ccgttgatga caccggttat ctgactgtcc
    53041 cgctggaaga tattctcgaa agtatgggcg atgaagagat cgacatcgac gaggttgaag
    53101 ccgtccttaa gcggatccaa cggtttgatc cggtcggtgt agcggcaaaa gatctgcgtg
    53161 actgcctgct aatccaactc tcccaattcg ataaaaccac gccgtggctg gaagaggcca
    53221 gactgatcat tagcgatcat ctcgatctgt tagccaatca cgacttccgc actttaatgc
    53281 gcgtcacgcg tctgaaagaa gatgtgctga aagaagccgt caatctgatc cagtcgctcg
    53341 atccgcgccc cgggcagtcg atccagactg gcgaacctga gtatgtcatt ccagatgtgc
    53401 tggtgcgtaa gcataacggt cactggacgg tagaactcaa cagtgacagc attccgcgtc
    53461 tgcaaatcaa ccagcactac gcctcgatgt gcaataacgc gcgcaacgat ggtgacagcc
    53521 agtttatccg cagcaatctg caggatgcca aatggttgat caagagtctg gaaagccgta
    53581 acgatacgct actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc
    53641 aaggtgaaga atatatgaaa ccgatggtac tggccgatat cgcccaggct gtcgaaatgc
    53701 atgaatcgac gatatctcgc gtgaccacgc aaaaatacct gcatagtcca cgaggcattt
    53761 ttgaactgaa gtatttcttt tccagtcacg tcaataccga gggcggcggc gaagcttcct
    53821 ccacggcgat tcgtgcgctg gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt
    53881 tgagcgacag caagttaacc tctttgctgt cggaacaagg tatcatggtg gcacgccgca
    53941 ctgttgcgaa gtaccgagag tctttatcca ttccgccgtc aaaccagcgt aaacagctcg
    54001 tttgacccaa ccgataagga agacactatg cagctcaaca ttaccggaaa taacgtcgag
    54061 atcaccgagg cactgcgcga atttgttaga gccaaatttg ccaaacttga gcaatatttt
    54121 gaccgaatca accaggtcta tgttgttctg aaagtggaga aagtcaccca cacctcagat
    54181 gcaacactgc atgtaaacgg tggtgaaatt catgccagcg cggaaggtca ggacatgtac
    54241 gctgccattg atggcttaat tgataagctg gcacgccagt tgaccaaaca taaagataaa
    54301 ctgaaacaac actaattgtc cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac
    54361 aacgggccat ttgtacggtt aatgctccga gcctgttcca ctgtttgagt gggcaggttc
    54421 ttaggtgaaa ttatgacaaa taatgataca actctacagc ttagcagtgt tcttaacagg
    54481 gaatgtacgc gaagccgcgt tcactgtcag agcaaaaaac gcgccctgga aatcatcagc
    54541 gagctggcgg cgaaacaact tagcctgccg cctcaggtgg tttttgaagc tatcctgacg
    54601 cgggaaaaaa tgggcagtac cggtatcggc aatggtattg ccattccgca tggcaaactg
    54661 gaagaagata ctctgcgcgc cgttggcgtt ttcgttcagc tcgaaacgcc tatagctttc
    54721 gatgccatcg acaaccaacc ggtggactta ctttttgccc tgctggtgcc ggcagaccaa
    54781 actaaaacgc acctgcatac tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc
    54841 tgccgccgtt tacgcgcagc ccagagcgat gaagagctgt atcaaatcat tacggatacc
    54901 gaaggtactc cggatgaagc gtagttattc ggtaatgtct cttttagacg ttgtgaggag
    54961 aaacagtaca tggtactgat gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc
    55021 ctgcgtgcgc tggaagatat gggtttttac tgcgtggata accttcccgt agtgttgtta
    55081 cccgatctgg ctcgaaccct ggccgatcgc gagatttctg ccgccgtcag cattgatgtt
    55141 cgtaatatgc cggagtcacc agaaatattc gaacaggcga tgagtaacct gcctgacgct
    55201 ttctcaccgc aactcctgtt cctggatgcc gaccgtaata ccttaattcg tcgttacagt
    55261 gacacgcgcc gactgcatcc gctttccagc aaaaacctgt cgctggaaag tgctatcgac
    55321 aaagaaagcg atttgctgga gcctctgcgt tcgcgagcgg atctgattgt cgatacctca
    55381 gaaatgtccg ttcacgagct ggctgaaatg ctgcgtaccc gtctgctggg taaacgtgaa
    55441 cgcgaactga ccatggtctt tgagtctttc ggcttcaaac acggtatccc tatcgatgca
    55501 gattacgtct ttgacgtgcg cttcttgccg aacccgcact gggatccgaa actgcgtcca
    55561 atgacaggtc ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga agtacacaat
    55621 tttatctacc agacgcgaag ctatcttgag ctatggttac ctatgctgga aaccaacaac
    55681 cgtagctact tgacggtcgc cattggttgt accggcggga agcaccgttc ggtgtatatt
    55741 gcagagcaac tggcagacta cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt
    55801 acgctggaaa aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca aacaagctgg
    55861 gcatgcatgc ccggcctgca atgaagctgt ttgaattaat gcagggtttt gacgctgaag
    55921 tgctgttacg taatgacgaa ggcaccgagg ccgaagccaa cagcgttatt gcgctgctga
    55981 tgttggattc tgccaaagga cggcagattg aagttgaagc gaccggccca caggaagagg
    56041 aagcactggc cgccgttatc gccctcttta actctggttt tgatgaagat taatcttcat
    56101 cactttgaca tacaactccc ttcaaactcc ccccgacaat aagaaaatca cgtactgaaa
    56161 tcgttctcaa tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag aagaacctta
    56221 agaaaacctt aagaggcatt gtttaggttt tgtttaagtt aatcgaccat actggagatc
    56281 gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca
    56341 ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg
    56401 tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgt
    56461 atcttggtcg ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga
    56521 cggattgcgg aaccggttat gtctacgatg tgattgcaga ttttgacggc aaaccctcca
    56581 ttacgctgac cgaatttgct gaacaatgcc gttacgaaga agacattgca caactgcgcc
    56641 agctactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga
    56701 agccgcagaa tatcctctgt caccacatca gtgagtcaga ggttatcccg gtcgtctgcg
    56761 acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc
    56821 gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc
    56881 agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat
    56941 ccagctggtg ttgttgcata aacggctcac cgcctaactg atacatctgc cgtaaaatcc
    57001 acgcctgacg gctacgcacg tagcccgatg gcgaggagac tttgaaacga agtggattag
    57061 gtaatacagc tgccagtaat gcagcttccg accgggtaag tttgctcgcg ggtttgtgga
    57121 aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca
    57181 ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccag
    57241 cttccagccc ttttcgtacc cagctacgcc catcccataa aaagagattt ttggccgtct
    57301 gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg
    57361 ctttctcaat ggaagcgaca tcaaagcccc ggtgctcagg aaatttctga tcttctgcgg
    57421 caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg
    57481 ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc attaccgctg
    57541 agaagggaac aggcgcaaca ctaaacaacg cgatgccccc gccccagaaa acggcgagga
    57601 caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac
    57661 tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc
    57721 aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt catcaaccac
    57781 gatatcatcg acaggacagg gcacatgctc cgcgcccatc tcttccagca cttctgcggt
    57841 atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc
    57901 cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc
    57961 ctttaattca cggtcaacgg tgcattcgct accaagagtg gcaaaattgc ttaaattctt
    58021 cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc
    58081 cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacgt tgcgcgtttc
    58141 cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa
    58201 gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat
    58261 ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat
    58321 catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt acgcatcaca
    58381 catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct
    58441 gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc
    58501 cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt
    58561 ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc atctcttcaa
    58621 tccattcacc gacgttatct tcccaggcag gaaggtcagg agactgaatt tgctgaccca
    58681 gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta tgtccttcct
    58741 caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg
    58801 gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagttcga
    58861 gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg
    58921 tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg
    58981 ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt
    59041 tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac
    59101 gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca
    59161 acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga
    59221 catcaacgct gttacctaat ttttccagca cagaacgcgc aacaatcacg ttcagttcaa
    59281 tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat
    59341 catcgacctc ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc
    59401 cctgttcgct ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg
    59461 ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat
    59521 acatggcgaa aattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt
    59581 caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg
    59641 tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc gtcccgtcgg
    59701 taatgacctg atgcggtaat ggcagcgtcg gctccaggtt aaagcgcaat cctttttgtt
    59761 gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggtt
    59821 gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata tcgttaaaga
    59881 tattccccag cgtgacggcc gaaacatgga tggtcttgag atatttttcc tgctcggcgg
    59941 tgagttcggt atccagcaga atgcggctca gaccgacgat accgttcagc ggtgtacgca
    60001 attcgtgact gatggtggag ataaacgtcg ttttgtcgcg gctggcccgt tcaagcgcat
    60061 cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac
    60121 ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc ccgtccgggt
    60181 aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt
    60241 caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg tgaaccagtt
    60301 gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat
    60361 cttcgttacg ataaaaaacc aggtcgggtg aagcatcaag gaaggaacgt aagaatgagg
    60421 attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc
    60481 cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc tgatttagct
    60541 gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca
    60601 gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg
    60661 atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat
    60721 caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa
    60781 gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac ttcatcatca
    60841 ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca
    60901 acctgcccca ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga
    60961 attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg ccgatccctg
    61021 tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc gattttcgca
    61081 ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac
    61141 ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat
    61201 accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca caatatgcag
    61261 cggatgcagc tttatgccgt ccacgccggt ttcaaccact cgctccagcg tttgcaaaca
    61321 ttcagcctgc ccttcaccag gcaggccgac aattaagtgg gaacacacct tcagcccgcg
    61381 ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc ggttgatgcg
    61441 atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtaaccctg
    61501 gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc
    61561 aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag aacgcagcac
    61621 ctgaacttcc gcaaaggtgc tagtatacgc ctgaaagtag gccagatagc gtttagcgcg
    61681 gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc
    61741 atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg taccgtcacg
    61801 gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc cataacgacg
    61861 ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc
    61921 gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga cctggatcaa
    61981 tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcat
    62041 aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt
    62101 aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa tgctggtttt
    62161 ctcgtcagtt caaggtagga taagggttaa cacgccttta tgacagtcag gaattgactg
    62221 tttctctaac gacttccctt ttagccttaa agataaaatt cattttaatt tcagttgctt
    62281 aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta
    62341 tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat
    62401 catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa
    62461 caagggggcg aatacgaggc gcgcgtatga cacgcaaccc ccgtcgccac gctctttctg
    62521 tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata
    62581 tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag
    62641 aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc
    62701 accgtggcgc gattctcgcc gatggtaaaa ccggtgacgg ttgcggcttg ctgttacaaa
    62761 aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact
    62821 acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgcggctgcc gcacgccgca
    62881 tcgttgaaga agagctgcaa cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca
    62941 ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccccgcatt gagcaaattt
    63001 tcgttaacgc tccggcaggc tggcgtccgc gggatatgga gcgccgtctg tttatcgccc
    63061 gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga
    63121 atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc ttttatctgg
    63181 atcttgcgga cctgcgtctg gaatcggcca tctgcctgtt ccaccagcgc ttctccacta
    63241 acaccgtacc gcgctggccg ctggcgcagc cgttccgcta cctggcgcat aacggtgaaa
    63301 tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc
    63361 cgcttatccc tgatctgcac gacgccgcac cgttcgtcaa cgaaaccggc tctgactcca
    63421 gttcgatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc atccgcgcca
    63481 tgcgtctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg gaactgcgtg
    63541 ccttctttga ctttaactcc atgcatatgg agccgtggga tggcccggcg ggcatcgtga
    63601 tgtccgacgg tcgttttgcc gcctgtaacc tcgaccgtaa cggtctgcgt ccggcgcgct
    63661 acgtcatcac caaagataag ctcatcacct gcgcctctga agtcggtatc tgggattacc
    63721 agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg gttatcgaca
    63781 cccgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa agccgccatc
    63841 catataaaga gtggatggag aaaaacgtcc gcagactggt accgtttgaa gatctgcccg
    63901 atgaagaagt gggtagccgc gaattggacg acgacacgct tgccagctac cagaaacagt
    63961 ttaactacag tgcggaagag ctggactccg taattcgcgt actgggcgaa aacggtcagg
    64021 aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt cagccgcgca
    64081 ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca atcgacccgc
    64141 tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt
    64201 gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg ctctactccg
    64261 atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg ctggatatca
    64321 cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctgtgc gacaaagccg
    64381 aaaaaatggt acgtagcggc accgtgctgc tggtgctctc cgaccggaat atcgctaaag
    64441 atcgcctgcc ggttccagcc ccgatggcgg ttggcgcgat ccagacccgt ctggtcgatc
    64501 aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc
    64561 acttcgccgt gttgctgggc ttcggcgcga cggctattta tccatacctt gcctatgaaa
    64621 cgctgggccg cctggtagac acccatgcga ttgccaaaga ttatcgtacc gtgatgctca
    64681 actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca
    64741 ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac gatgatgtag
    64801 tgggcctgtg cttccagggg gcggtcagcc gcattggtgg ggcgagcttt gaagacttcc
    64861 agcaggatct gctgaacctg tcgaaacgtg cctggctggc gcgtaagccc atcagccagg
    64921 gcggcctgct gaaatacgtc cacggcggcg aataccacgc ttacaacccg gacgtggtgc
    64981 gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa tacgcgaagc
    65041 tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg ccgggtgaaa
    65101 acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc tttgataccg
    65161 ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga
    65221 atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg cgctatggca
    65281 ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt actccggcgt
    65341 atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag ccgggcgaag
    65401 gcggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc tattcggtgc
    65461 ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc gaggacttag
    65521 cgcagctcat tttcgacctc aagcaggtta acccgaaagc gatgatctcc gtgaagctgg
    65581 tttccgaacc gggcgtaggc accatcgcga ctggcgtggc aaaagcttat gccgatttaa
    65641 tcaccatcgc aggctatgac ggtggcaccg gcgcaagccc gctgtcgtca gtgaaatacg
    65701 caggttgccc gtgggagctg gggcttgttg aaacccagca ggcgctggtt gccaatggtc
    65761 tgcgtcataa gatccgtttg caggtcgatg gcggcctgaa aaccggtgtt gatatcatca
    65821 aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg gtcgcgctcg
    65881 gctgtaaata tctgcgtatt tgccacctga acaactgcgc aacgggtgtg gcaactcagg
    65941 atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgacg aattactttg
    66001 agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggggtaacg cggctggtgg
    66061 atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc aaacagcaga
    66121 aactggcgct gtcgaagctg ctggagaccg ccgaaccgca tccaggtaag gcactctact
    66181 gcaccgaaaa caacccgccg tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg
    66241 cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc cgcaataccg
    66301 atcgctccgt gggcgcgtcg ctttcaggct atatcgccca gacgcacggc gatcaggggc
    66361 tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt ttcggtgtat
    66421 ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat gtcggtaaag
    66481 gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc cgcagccatg
    66541 aagcaagtat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg
    66601 caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc gtggtagaag
    66661 gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt ctgggtaaaa
    66721 ccggcgttaa cttcggtgcg ggcatgaccg gcggcttcgc ttacgttctc gatgaaagcg
    66781 gtgatttccg caaacgcgtt aacccggaac tggtcgaggt cttaaacgtt gacgatctgg
    66841 cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat actggctctc
    66901 agcgcggtga agagattctg gcgaactggt caaccttcgc cactaaattt gcgctggtta
    66961 aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc gcagcagagt
    67021 tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg
    67081 acctgcagcg cgttgatccg cctaagaaac cgctgaagat ccgcaaaatt gagtttgttg
    67141 aaatttacga gcagttttcc gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt
    67201 gcggcaaccc atactgcgag tggaaatgtc cggtacacaa ctacatcccg aactggctga
    67261 agctcgccaa cgaggggcgt atttttgaag cggcggaact gtcgcaccag accaacaccc
    67321 tgccggaagt ttgcggacga gtctgcccgc aagaccgtct gtgcgaaggt tcctgcactc
    67381 tgaacgatga gtttggcgcg gtgaccatcg gcaacattga gcgttatatc aatgataaag
    67441 cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt aaaaaagtgg
    67501 cgattatcgg cgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg cgtaacggcg
    67561 taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc ttcggtattc
    67621 cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc accggcatgg
    67681 gtattgaatt caaactcaat accgaagtgg gccgcgacgt gcagctggac gatctgctga
    67741 gtgattacga tgccgtcttc cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg
    67801 aaaacaaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac
    67861 agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg
    67921 tggtggtcct tggcggtggc gacactgcga tggactgcgt gcgtacgtcc gtgcgccagg
    67981 gagcgaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg ggttcccgcc
    68041 gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccactgg
    68101 ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt accgaaatgg
    68161 gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc gaacatatcg
    68221 ttccggcaga tgcagtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg
    68281 caaaacacag cgtcgagctg gattcacaag gccgcatcat cgccccggaa ggcagcgaca
    68341 acgccttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg
    68401 atctggtggt aaccgctatt gccgaaggtc gtaaggcggc agacggtatt atgaactggc
    68461 tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct
    68521 cctttctttc tggaaagcgc ctcgcataat ctgaagaaaa ccaatgtaat acgctcatct
    68581 ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg cagcgccata
    68641 taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac cagcaaatcc
    68701 acgacggtat tattcacctg gtcgatagcg cccggacgga aacggtacgt agcgttaacg
    68761 cgctgatgac cgcgacatac tgggaaattg gccgacgaat tgtcgaattt gaacaaggtg
    68821 gcgaggcaag ggctgcgtat ggtgcgcagc taatcaagcg actatcaaag gatttaagtc
    68881 taaggtataa gcgtgggttc tctgcaaaaa acttacgcca aatgaggctt ttttacctct
    68941 tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca ttgggaattc
    69001 cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg tctgccaaat
    69061 tgagaggctg tttgatattt ccacagcttt tgtattgcat ctcctatttt cggtttgtat
    69121 attcatgcaa ataaacgaag gaaagttgcc atgatttaca ttgaacagct tgaattaatc
    69181 cataaaagtg gtgatgtgtt gtatccagta aaaataacca gaaaatcatc aggaaaaact
    69241 gccttccatc ttgttccatt cggcttaaat aagactcacg accttctaga ggtggaagat
    69301 gcttcagaag caattcggct ggtgattgat gaacgtcatt ccattcgctg cagtacactt
    69361 acagcaacta ttacgaataa aaagggcaag cgaattaagc ggacaggtat atacagtatc
    69421 aagggtgtta acataaaaga atacaacgtc aggtaatggc acagagcggt ccgtcagttc
    69481 aggtttggct ctgtgccaaa gatgggtcag gtcacacctg atctaataaa tttaagccat
    69541 taaatcagcc ttaaccttca ccaccacctt tttaatctca cccggctcgc cgacaacgca
    69601 tcctggttta tgcggttcac ttggcataaa cacggcgaac attcccggtt ttaagatgat
    69661 ggcttgctcg ttatcaatgg tgctgcaaag ctgataatca tcctcatggt ggaactcttc
    69721 acactggcgc gcagtgcctg ccatgccaaa cagaatccgt tcctcaccgt ttaataacag
    69781 ctggatatca atgtattgct cgtgcaattc cgcttttttc tcgacgggcg attgagtgtt
    69841 aaacgtcatg acattcataa agatattgtc gccctgtaat tcgtaacgac caggcgcttt
    69901 ttcttgcggt ctggcagcta atgccagcgt taacgcgtcc tgtaacgcag gatgtaaccc
    69961 agcagacggt aatgactgta cttcacccat catcataatt tttctccctg ggccaacagc
    70021 gcagccccaa gtaaacctgc atcgtggcgg tagtgcgccg ccagtaaatc aacatgaaat
    70081 gccgctggct cctgcgccag atacgtttcc accaacgcca gatacccttc tgccagacca
    70141 acgctgccac cgaccaccac gcactggcaa tcagttgtgg ctttaatatc agcgatcagc
    70201 cttgccagca catgtgcgga gcggtgaatc agttgctgcg cctgctcgtc gccctgcccg
    70261 gcgcgtgtga aaatagttct cgcatccgcg cccatgagtt caccttgcgc tgccgctgca
    70321 ataccgcgac cagaagcaat cgcttccacg caacctgtgc gtccacagcc gcagactggg
    70381 ccgtgtggat cggcaagcgt atgcccgatg tgccccgcca gaccgccagg gccggtaagc
    70441 agtttaccgc cgctcactac accgccgccg acgccagtgg aaacggtgat aaacaccata
    70501 tcggttatat cgccttccag cgcctgatac tctgcccatg ctgcggcctg cgcgtcgtta
    70561 atggcaatgg tcggcaaatt ggaaagttgt tccagcgttt tgactaacgg aaagtgtagc
    70621 aatccaccaa gattatgcgg attcagcgcc agcaagctgc cgtcgcgaat aattccggtt
    70681 gaagcgatgg caacctgctg cgcatgagct tgcaacgggg cgactaacgc ggctaaggca
    70741 tcacgcaacg cctgcggagt ctggctggct ggtgtcggaa gttctcggcg atcgcggatc
    70801 tgcccgtcag cgccaatcag cgcggcggca agtttagtac cgccgatatc aatcgccaac
    70861 gtggtcatag caccgccttt ttcatcgctg tgttgtacca ctgacaaatg tgttcaagac
    70921 gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg gcgcatcgca tctgccgcct
    70981 gagcaggcgt gttgtaacgc ccttcggcaa tcacccgaca tccggcgtcg ctcaacgttt
    71041 tcaccagcgc cagatccggc tcttctggcg tttcaggcgt ggtatagcca gaaagcgtag
    71101 tgccaataat ttcggctccc agcttttggc atgccaggcc gtcttccggc gttgagcagt
    71161 cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag cagcgtttca acaggcaccg
    71221 gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc gcccgcctgc gccagcgcat
    71281 caacatcttc aatataggcc gtgatgcgta ccggagaatc ctccagatcg cgtttcacaa
    71341 ttccaataat cggcacgctc accaccgcac gcgtggcttg cagatttgcc acaccttcaa
    71401 tgcgaatggc aaccgcgccc gcctgttctg ccgctaatgc catggcagcg acgatttcgg
    71461 gtttatcgag cgggctgtcc ggaaccggct ggcaggagac aatcaggcca ccgttagcag
    71521 cgattttttg atccagttgt gcaagtaacg acatacatct tcccttagcg aaaggcccgg
    71581 tacagagacc gggcaacagg attaactttt ggttttgact aaatcgtttt tggcgctgcc
    71641 aaatggcacg gcaccgctaa atggtttgcc gtcgatagcg tcatgagtac gcaatgcttc
    71701 cgggcgcagc caacgctgaa cgcgggaagg catatccaga ccaatcagca ggatcaccac
    71761 aaatgtcaga ctgaacgata gcgatgccag cgcagtaccc agatccagac gttgagcgat
    71821 caacgcgccg atgattgggg ccagtgcgcc gcccaatgcg ccaacgttgt aggtaaagcc
    71881 caggcccgct gcacgctggt cggtatcgaa ataaccgcca atcagtttcg gtaagatccc
    71941 ggcgatccct tgtccaagca tttgctggaa gaacagtaac agaccgagca cccagacgtt
    72001 tgcgccgcca atcgcaaata ccggaataat caacagctgc gaggccaaca ggctacaaac
    72061 gtaggctttg cgggttccca gccagtcacc gaggaagccg ctgacacagc accccaccgc
    72121 agccccaaag ccgctaaaga acagcacatt ggcgacagta tgcgggttat aagccagatc
    72181 ggttttcaga tacgttggca gcagcgcctg aatcggccat gagtagagga aagcaaacaa
    72241 cacgaccacc atcagcatta cgcccgttgg ccagcgttta cccgtacttt gcaccataaa
    72301 gctgataaag attgcggcgc ataacagccc aagaacagcg acgatcgcgg cattttgcag
    72361 gttaccggcg aagcaaaacc acagcgcagt agccgccgcc agtgtcatta cgatattggc
    72421 aatgcgatgt tcaccacggt agagaatatc taccatcgtg cgtactggtg ctttacctgc
    72481 atgcttctct ttccagtctt ccgcttccgg gatgttttta cgcagccaga gagcaaagat
    72541 gataggcaaa atgccgataa agaacagcgc acgccagccc cagaccggaa ccaccaggct
    72601 atagacctga gcggcaacga cggcccccac agagaagcct gaaatcagaa aaccactggc
    72661 tttgttacgc agatgttttg gccagctttc aatgacatag gtggcgctgg aaccgtattc
    72721 acccgccatc cccatgccga tgaccagacg cgcgataaac atggtgatgt agcctggcgc
    72781 aaagccgcag gccagcgtcc cggccgagaa gagaacgatg ctggtgacca ttgccagacg
    72841 acgcccgtag cggtcaccca tagcgccgag catcaggccg ccgaaccagc gagagataaa
    72901 ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc ccgaattcac cctgtacttc
    72961 ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca agcagatatc ccaaccaggc
    73021 agcggaaaat gcgcgccatt gtgcacggtt gagatggcga taccacggga tattctgggt
    73081 tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc acacgctttg ctataaaaat
    73141 tgtagggtac agatgcgttt atttcccctc acccggtagg ggcgagcgag gggaaacaac
    73201 tcacccgcgc tcttgcatca actgctgggc cagcgccttc agttctggca gatatttttc
    73261 atctaccggt ccaaacggtt tgcggcacag cggcacagaa acgacatcca tataatggag
    73321 gacagttttc aggccgcgga atacgcccgt tttgatcagt aaatcaatga ctttattgca
    73381 ttcagtttgc agtttctgcg cggtctggat atcgccttct ttcagcgcct taacgatccc
    73441 ctgatagcgc cagcccatga tgttgtaggt actaccgata ccaccatcag cgcccgccag
    73501 cagaccagag gcgaagattt cgtcgtaacc gttatagagc acaagatcag gatgttcacg
    73561 acggatctgc tccatctgat agagatcgcc agaggtctgt ttcagcgcac ctacgccagg
    73621 caatgtaaca agtgtgttga tctgatccag ggtcagtttt accccactca gggctggaat
    73681 gttgtacacc accatcggca aaccatccgc cgaatcaata attgcccgat agtgatcgca
    73741 gtgttcttca aagctgaaag gatagtagaa cggcgtgacg gcggagacgg catcgaagcc
    73801 ataacgttta gccgatgccg caagttgttg gctttcggcg gtgctgacgc aaccgacgtg
    73861 ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg atttccagta cctgttcacg
    73921 ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca cccacgtata aaccgtcgat
    73981 gccctgctga atattgaact gaaccaggcg acgcagactc gctttatcca gtgcttgttg
    74041 ttggtcaaaa ggagtcagga gtgcagccat tacgccacgt aaattcgttg ccataaatac
    74101 ctctgaagtg atgcttgtct gataaacgat atacctttat acctgttata ccagatcgat
    74161 taagcaacac cccatacaga aagcttataa tgcgatctgc ttcactaaag tggcattatt
    74221 tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg
    74281 attgcaacgc acgatcggct tcgtcaggat catgacggcg gatcgcatca acgatcgcaa
    74341 tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc
    74401 gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttaccgg
    74461 ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat
    74521 ccagcgactg actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg
    74581 catgttcagc cgcatagcgc accagactgg attcaaagaa cagacgtaat tgttcgaaat
    74641 gggcaatccc accgggatga gaaaggaaat ctttcgccat gccggaaagc tcaccgatga
    74701 tagtgtccgc agaaggacgc gagacgcgag cgcgttcgcc gttgtttatt tgcaccagac
    74761 ctttgcgttt taacgctgcc agcgcttcac gcaccgaagg acgcccgacg ttaaagaacg
    74821 ccatcagttc gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca
    74881 tctgttccag ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctac
    74941 gcaagttgcg accaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa
    75001 ggcccattct gtaaggtcag tgtgattaac atcatcagtg acatcctatc acaggattga
    75061 gagtagggga aaatggcagg tttttctctt tgtgcctcat cattaccata attaacggaa
    75121 taattaacta ttgcgaaaaa ttaatgtaac gcagataaaa acatcccgtt tgaattattt
    75181 ataagactat tcacgagcat tatgaatatt atgaatgcgt tcttgcaaaa taatcataag
    75241 cgcatatttt ttaatgaaaa attatctcac ctacaattaa aaacacgaca tccgcaccat
    75301 aaatagactt gcaaaaaata taacatcatt gttttcaatc tgccgtttat gggattgacc
    75361 gttttctttt gacacggagt tcaacaatgt tcggcataat tatatctgtc atcgtattaa
    75421 ttacgatggg ctatttgatc ctgaaaaact acaaacctca ggtggtgctg gctgccgcag
    75481 gtatcttcct gatgatgtgc ggtgtctggt tagggttcgg tggtgtactc gagcccgcca
    75541 aaagcagcgg ctacttgatc gtcgatattt ataatgaaat cctgcgcatg ctgtccaacc
    75601 gcattgccgg attggggcta tcgattatgg cggtgggcgg tatgcccgct acatggagcg
    75661 cataggggcc agtcgcgcga tggtgagctt gttaagccgc ccgttaaaac tcattcgctc
    75721 accgtatatt attctgtcgg caacttacgt catcggtcaa atcatggcgc agtttattac
    75781 cagcgcctcc ggtctgggta tgttgctgat ggtcacctta tttccgacac tggtgagtct
    75841 gggagtaagt cgtctctctg cggtggcggt tatcgcaacc acaatgtcca ttgagtgggg
    75901 aattctggaa acgaactcca tttttgcagc ccaggtcgcg ggaatgaaaa ttgccactta
    75961 cttcttccac taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg cgatctccca
    76021 ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat atcaatcacg aacaagcaga
    76081 gcaaaaagct ctcgataatg tcccgccgct ctattacgcc attttacctg tgatgccgtt
    76141 aatcctgatg ctcggctcgc tgttcctcgc ccacgtcggg ctgatgcagt cagaactgca
    76201 tctggtggtg gtgatgttac tgagtttgac ggtgacgatg tttgttgagt tcttccgcaa
    76261 gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt tttgacggca tgggtacgca
    76321 gtttgccaac gtggtaacgc tggtggtcgc gggtgaaata tttgcgaaag gcttaacgac
    76381 gattggcact gtcgatgcgg ttatcagggg tgcggagcat tctggtctgg gcggtattgg
    76441 cgtgatgatt attatggcgc tggtcattgc catttgtgcc attgtgatgg gctctggcaa
    76501 tgcgccgttt atgtcatttg ccagtcttat tccgaatatc gcagccggac tacatgtacc
    76561 agcggttgta atgattatgc cgatgcattt tgccacgacg ctagcgcgcg cggtttcgcc
    76621 gattactgcg gtggtggtcg ttacgtcagg aattgcaggc gtttcgcctt ttgcggtggt
    76681 gaagcgaaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg ccacaatcac
    76741 gctattttat taagccatta aaagacaaaa caggccacca gggcctgttt ttgtattact
    76801 tcacaacgcg taatgccggt cgaccaccgc gtggtggctg cggaggttca tcgtcaggat
    76861 gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg gtttcgttgt
    76921 ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca gcttcaggct
    76981 caaacattgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca gccagcggca
    77041 cagaaacctg acgcggaatg ccgccaaagc gcgcgttaaa gcgcacctca tcattcgcca
    77101 gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat
    77161 attccatagg aacctgcacg ccagggagcg tcacatccac cactaggtgc ggcgtgagct
    77221 ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt ggtgttagct
    77281 gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg cttcagttaa
    77341 agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca gctctttcgc
    77401 acccgggccg ctgaactcga tgcccagttg cggcagacgc cacagcagcg gagcaagata
    77461 gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga agaccggcgc
    77521 aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag aagctgaacc
    77581 gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca tgtacagacg
    77641 gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga aacgctcatc
    77701 cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca ccagggtcgg
    77761 aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct tttccacgtg
    77821 ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat ggctatagat
    77881 gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga cagccatgaa
    77941 aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc agtcagaagt
    78001 tgtgttaccc agtaaggaac gactctcttt gttcgaaaat caaacaaaaa atgagcaata
    78061 cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc tttgtggtga
    78121 gtcgatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt gacttagtcc
    78181 cttattggcg atgtggtttt tgttttacct gtctgtcagg tggcagcaaa aagcaacttt
    78241 ccagttttta cgctgattca gattttagct ataaaaaaac ccgccgaagc gggttttttc
    78301 gaaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc ggacgacgac
    78361 gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga gtaacgaagc
    78421 cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg cgggtgatac
    78481 cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg atgtacaggt
    78541 ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg cgggcagttt
    78601 cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg ttgcccggtt
    78661 tgatgaaaac gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat tgattttcag
    78721 ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt
    78781 gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc gggccttttg
    78841 gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca atcatctctt
    78901 caaaggtcgc ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac actttgtcag
    78961 tacacttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg taatcaccgg
    79021 tatctacgtg cggagtgtat tccgctttgt gcttaccgcg caggcgacga gccagttcag
    79081 tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg
    79141 tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag ttacacgttg
    79201 gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg caaacctacc
    79261 ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaactttc ttgtaacgtg
    79321 gggtcgcagg attatagaga agtcggggtc aaagatcgac ccctttttgt gatttgtgac
    79381 aggttttaac ccgccaaatg ctcgcgcttc agatactctt cgctttgcat ctcttgcaga
    79441 cgtgacaggc aacgctggaa ctcaaacttc agccgatcgc cctgataaat ttcatacagc
    79501 ggcacttctg cactcaccac taatttgaca tggcgctcgt aaaactcatc caccagcgca
    79561 ataaagcgcc gcgcttcgct ctccatcaac cgcgtcataa ctggtacatc aaacaacatg
    79621 accgtatgaa agaggcgtga gagcgcaata tagtcatgct ggctgcgggc atcgacacac
    79681 aaggtggtaa aagagacagc aagcgtctga ttctcagcgc ccattgtcgc cagcggtcga
    79741 tggttgattt ctaacgtcgg tgaattttcg cgtttcgctc ccgccagcgc caaccatagt
    79801 ttatccattt gtgcccgggt ttcatcgtgg agtggcgaaa gccacagatg cgcctgagtg
    79861 agtgtacgca gtcgataatc aacaccagcg tccacgttca ttacatcaca atgctgttta
    79921 atggcatcga ttgcaggcag aaaacgcgca cgttgcaagc catttcgata aagttcgtcc
    79981 ggcggaatat ttgacgttgc taccagggta ataccgcggg cgaacagggc tttcatcaga
    80041 ccgccaagta gcatggcgtc ggtaatatca gaaacaaaaa attcgtcaaa acagagcacg
    80101 tcagtttcgg ctttaaagcg atcggcaata atttccagcg gatcggtctg cccctgtaag
    80161 gcagttagct cttcgtgcac ccgcagcata aaacggtgaa agtgcaggcg ctgtttccgc
    80221 tctcccggca ggctttgata gaaaaggtcc atcagccagg ttttcccgcg tcctacaccg
    80281 ccccacatat ataagccacg cactggcgta tgctttgtgt cttcgcgttt accccacagc
    80341 ttaccgaccc gcgccattag cccactcgtc ctgggggctg gtggcctgct attgatgagt
    80401 tcttgataaa taatttccag gcggctgacg gcctcttttt gaacgtcgtc gggttgatgg
    80461 ctgccttcat tgagcgcctt caggtattgc gatgttgggg taacgctttg catgatctta
    80521 ttattattcc ttgaataatc ggtgcgccgt tgttcacggt tgacgaaaaa aaggccgttc
    80581 tacactacgc gatatgcagt cgggattcca cttctgtgga attaacggtt atagtggcat
    80641 aatcagccgt aggcatggag cctgaagcca acaccctacg gaaacaaaag acaacgggag
    80701 atgttcatga cctgggaata tgcgctaatt gggttagtcg tcggcatcat tattggtgct
    80761 gtggccatgc gttttggtaa tcgtaaacta cgccagcaac aggcgttgca gtacgaactg
    80821 gaaaagaata aagctgaact ggacgagtat cgcgaagagc tggttagcca ctttgcccgc
    80881 agcgcggaat tactggatac catggcgcac gactatcgcc agctgtatca gcacatggcg
    80941 aaaagctcca gcagcctgct gccggaactg tctgctgaag caaacccgtt ccgtaatcgt
    81001 ctggcagagt ctgaagccag caacgatcag gcaccggtgc agatgcctcg cgactattct
    81061 gaaggtgcat ccggcctgct gcgtactggc gcgaagcgcg actaatttat ttttcgggcg
    81121 cagccattgc gccctcctct tctctccctc cccgactatc atttaatctg gtgtctcatt
    81181 gttagccgtc tgaaaattca ataacatcaa actgttttga atctcttttc ttatcattca
    81241 ggtacgagag caggaataat gaaaaaacaa acccagctgt tgagtgcatt agcgttaagt
    81301 gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt cgattccagg ccaggttgcc
    81361 gatcaggccc ctctccccag tctggctcca atgctggaaa aagtgcttcc ggcagtggtg
    81421 agcgtacggg tggaaggaac ggccagtcag ggacagaaaa tcccggaaga attcaaaaag
    81481 ttttttggtg atgatttacc ggatcaacct gcacaaccct tcgaaggttt aggttccggt
    81541 gtcatcatca acgccagtaa aggctatgtg ctgaccaaca accatgtgat taatcaggca
    81601 cagaaaatca gtattcagct caatgatggg cgcgagtttg atgcaaaact gattggtagc
    81661 gatgaccaga gcgatatcgc cctgttacaa attcaaaacc cgagcaaatt aacgcaaatc
    81721 gctattgccg actccgataa attgcgcgtc ggtgattttg ccgtagcggt cggtaaccca
    81781 tttggccttg ggcaaaccgc cacctctggc attgtttccg cattaggccg cagcgggttg
    81841 aatcttgaag gtctggaaaa ctttatccag acagatgctt ccattaaccg cggtaactcc
    81901 ggcggtgcac tattaaacct taacggtgag ttaattggca tcaacactgc aatccttgcg
    81961 cctggcggcg ggagcgtcgg gattggattt gccatcccca gtaatatggc gcgaacactg
    82021 gcgcagcagc ttatcgactt tggtgaaatc aaacgcggtt tgttaggcat caaaggcacc
    82081 gagatgagtg ccgatatcgc caaagccttc aaccttgacg tgcagcgtgg cgcgtttgtc
    82141 agcgaagtgt tgccaggttc tggctcggca aaagcgggcg tcaaagcggg cgatattatt
    82201 accagcctca acggcaaacc gctgaatagc tttgctgagt tgcgctctcg tatcgcgact
    82261 acagagccgg gcacgaaagt gaagcttggc ctgctgcgta acggcaaacc actggaagta
    82321 gaagtgacgc ttgataccag cacctcttcg tcggccagcg ctgaaatgat cacgccagcg
    82381 ctggaaggtg caacgttgag cgatggtcag ctaaaagatt gcggcaaagg tattaagatc
    82441 gatgaagttg tcaaaggaag cccagctgct caggcaggct tgcaaaaaga cgatgtgatc
    82501 attggcgtca accgcgatcg ggtgaactcg attgctgaaa tgcgtaaagt gctggcggca
    82561 aaaccggcca tcatcgccct gcaaattgta cgcggcaatg aaagcatcta tctgctgatg
    82621 cgttaatgtc gtaaaccggg catcaggctt acgtgtgatg tccggttaac tcgtggtatg
    82681 ctgctgccgt tccctttttt aatgacgcct ccatcatgtt tgtgaagctc ttacgttccg
    82741 ttgcgattgg attaattgtc ggcgctattc tgctggttgc catgccttcg ctgcgcagcc
    82801 ttaacccgct ttccactccg caatttgaca gtaccgatga gacgcctgcc agctataatc
    82861 tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta caaccgtggt gtgaacacca
    82921 actctcacaa ccagcttgag atccgcaccc tgggatccgg tgtaatcatg gatcaacgcg
    82981 gttatatcat caccaataaa cacgtcatca acgacgccga tcagatcatc gtcgccttac
    83041 aggatggacg tgtatttgaa gcattgctgg tgggatctga ctctctaacc gatctggcgg
    83101 tacttaaaat taatgccact ggcggtttac ctaccattcc aattaatgca cgtcgcgtac
    83161 cgcacattgg cgacgtagta ctggcgatcg gtaacccgta caacctcggg cagaccatta
    83221 cccaagggat tattagtgcc acggggcgaa tcggtctgaa ccctaccggg cggcaaaact
    83281 tcctacaaac cgatgcttcc attaaccacg gtaactctgg cggcgcgctg gtgaactcgc
    83341 tgggagaact gatgggcatt aacacgctgt cgtttgataa gagtaacgat ggcgaaacgc
    83401 cggaaggtat cggctttgcg attcctttcc agttagcaac caaaattatg gataagctga
    83461 tccgcgatgg gcgcgtgatc cgcggctaca ttggcattgg tggacgtgag atcgcaccac
    83521 tgcacgcgca gagcggtggt atagatcaac tgcaagggat cgtggttaat gaagtgtcac
    83581 ctgacggccc ggcggcgaat gcgggtattc aggtcaacga tctgattatt tcggtggata
    83641 acaaaccggc catctctgct ctggagacga tggatcaggt ggcagaaatt cgccctggtt
    83701 cggtgatccc ggttgtagtg atgcgtgatg ataagcagtt aacgctgcag gtcaccattc
    83761 aggaatatcc ggcaaccaat taagtcgagt gctcaaaaca aaaaaccgga gtctgtgctc
    83821 cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc agggcgatat
    83881 ctttcttgag cgtatccagc ataccttcca gcgcgttctg ttcaaatgcg ctcagggtac
    83941 cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga gagaagaaac
    84001 gggcgtactg accgtcgcct tcaacgtagg cacattcgac aacgccttgt tcgccctgca
    84061 gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac agggttgcag
    84121 acccgccacc ggctttcgct tcaaccacct cagtacccgc gttctggata cgtttggtca
    84181 gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc agcggcagaa
    84241 tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcttggc tgtttgcctt
    84301 tcagttccgc aacaaaggtg ttggaacgaa tgatatccag cgtggtaacg ccgaacagtt
    84361 tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaattgca actgtggtgt
    84421 taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca acttgctgta
    84481 ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc atacccggtt
    84541 tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc ggagtcgcat
    84601 cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga tcgacagcca
    84661 caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa ggcagttggg
    84721 tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg actgcgactt
    84781 tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct gccgcgacct
    84841 taatccacaa aattgccgtt tacaatgacc acatatcaag aatgtgtagt cacgcaagtt
    84901 tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt aacagtcgct
    84961 tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt cagccaaaac
    85021 aacatcaatt tgataacaat taatttactt ttaagcagaa tttgcatgcc gtgacgcagg
    85081 catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt atttcagcct
    85141 tcttcagggt tgactgtttg cataaaaatt catctgtatg cacaataatg ttgtatcaac
    85201 caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt taaagcattt
    85261 aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc cgcgttgcag
    85321 gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac caagtttggt
    85381 gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc tgaactgggt
    85441 gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta caacgatgca
    85501 gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct gctggactca
    85561 ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat ctttaccacc
    85621 cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct gttcgaccag
    85681 gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt atccctctca
    85741 accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttactgc gacatgtcat
    85801 tcacataatg aatacatacg gtaaaaaaag cacattatgc aaaattcatt atctaattga
    85861 aaatactaga attaaaaata aatgaccgtg tttttaattc ttttttgtta ttaaaattca
    85921 catttttaac acttagtatc aactgaaaca gttagcgtgg tattaattag ttcaataatt
    85981 agtgtatgct tgattttgtg atatgggtca caaaacaaag gcccagctaa aagattatgt
    86041 cgaggtaaaa atcatgaaaa tcaaaaccac tgtagctgca ttaagcgtac tttctgttct
    86101 ctctttcggt gcattcgctg ccgactccat tgatgctgca caagcacaaa atcgtgaagc
    86161 aatcgggacc gtatccgtaa gtggtgtggc gtcttcgcca atggatatgc gtgaaatgct
    86221 gaacaaaaaa gcggaagaga aaggcgcaac ggcctaccag attactgaag ctcgtagcgg
    86281 tgacacctgg cacgctacgg ctgaactgta caaataaacc ctcatcgtct tgtccgacga
    86341 tattgccccc ggttcggggg ctttttttgc gctaatgacg aacattaaaa cgcaaatgcc
    86401 cttccagctc ttcctctgcc tcatcaaaca gcaatattaa cgcgccaaaa cggcgacgcg
    86461 ttttctctcc cagatgaaca aattcaatct caagtggtag cggcaggaca tcgttcatta
    86521 acacatccca tagtgagtcg agatcgcgta ccttatcttt cgccagacca aaggtttggc
    86581 taaagtcacg ataaaaatcc tcctgactct caatctcatc aaaatcaaag gtataaatat
    86641 tcatctattg ccaccgtcac gtttcgcggc gagttctgcc gtgcatcgct aagtatagcc
    86701 atgaaaaaac cgacactttt ggcgtcggtt ttgacctaac tatcggtcaa cgcatgttga
    86761 tacttatgga gcatacccgc cagccgatga accggttccg tcacctgcgg tggcgcttgc
    86821 cagatgcgta gcttttcctg gtagatttca agttcttcca gcaactggcc aaagtaccga
    86881 cgacgtttat catcgctacg ggcagatatc acatggtctg ctgtgcggcg catttgtcgg
    86941 tgaaacgccg ataaatcctc gttaaccggg atcggcgcat cacgcaggcg ctggtgcgcg
    87001 ataatcatcg tcagcgccag gcgaaatttc ggcaaatccc ctgggaactt attcatcagc
    87061 aaaaacagct gctgataaag tgccgggagg tggttctctt tacgacgtgc cacattggta
    87121 gtcatcgcgg aaacagcggc agaaacaaac tgattaagca gtacacgttc ggtcctgtcg
    87181 cgcgatttat cccgcacaag caaaataacg gtgaacgcga gcacacagcc gacgatttgc
    87241 cctaatgcgc tgtcgagaaa ctgactgaaa tggaaagtca tcgggttatc cagcacgata
    87301 atatttatgg tgctggccag cgcccccatc gagcccagtc gccgttgctg tacttctata
    87361 ccgaggaaaa atcccagcac tgccaggcta atacacagca gcaacatgct ctgttgggta
    87421 ttagggataa tcaccaaaaa gtagagcagc cctaacggca gcgcggccag cgtcccgtag
    87481 ataaagtcga tcgccaccat gcgtggattc ggcaaacgca ttgccagtga cgtcactacc
    87541 gcaatcatca ccattgcacc actgccggaa gtccagcccg tccacagcca gaaaagcgtg
    87601 cccagaatgc aggaaagtgt ggttcgccag aagttaacca tcgcatgatg acgttcggct
    87661 gactctactt tgacttccgg ttcgccttgc aggatctctt cttcggtggc gttgattttt
    87721 gtgttactga taacgccgcg cttgagaagc tgataacgcg ttgccgccgc gacccagcta
    87781 taaatggtga caggcgtttc ccgttccccg gtccaggcga taactctccg caggcgtttg
    87841 agctgcttgt ggacgtcctg cgcggtttct accggcgtgt caaaaaattc gcggaaagta
    87901 tcagtgatca attccgggcg cgtattctga ataagataag tttcgcagga ttgggtaatc
    87961 agcgtcagcg atagcgtatt gatcgctttt aaacgtcgat tggcccgcgc ccagcgggaa
    88021 gattccatat tcaggttgct gcgcatgcct tgtagcgccg tggtgcgtcg caccaggtcg
    88081 ccccaggctt tatcgacaac ttcaccatcg ccatgcttga tacagagttg cattaattga
    88141 tattgcgcga ccagcaaact ttccagctct cgatccactt cttgtttgat cgatcgcgga
    88201 gaaaagagca aatccgctat aatcgcacac acaataccga taacgatctc gctacaacgt
    88261 tcgacggcaa actgcggcgt aagcaatggt tccggctgaa tggtgatcac aatgatcagc
    88321 gcggtataac cggccagccc ccacgcgtac gagttttcta ttcgtaccag cgaggatatc
    88381 caggtacaaa aaccggccca gatacagcac accagaatca tcaataatgg tgcgcggatc
    88441 atcgcaatga tgatcaccag tccggcaata cagccaataa atgtgccgat gatgcgcaaa
    88501 aagccacgat aacgaatagc gccagaatac ggttcacctc ccgcagcaaa ggccggaccg
    88561 gcggcaacaa tcgccgctgt cagtaccgcc cagcgtggcg tttccagctg gaagtgaaag
    88621 ccaacaaaca gcgccagtac aatggcggtc gccagtttta ccgcaaagcg aatatgttgg
    88681 ttagcaatgg agaaaatacc catcgtgatt aaccaaactc acgcaggcga tgggccattt
    88741 tacggaagaa cgaatcctgg ctttcgtcgc gatcttgttt gccagtgacc accactgtag
    88801 cagtggtgcc cgcaggccag atgttctctt gctggttgtc gagacgaata cgaaccggaa
    88861 cacgttgcgc aagacgcacc cattcaaggt tagagtctat agtcgccatc cctttgtcgt
    88921 cacgcgtgct gctggcgttg gtgacccctg cggcaacact atcaacagtc cctttcagca
    88981 ctttgttact gccaagcggc gtgatctctg cacgataccc cggacgcacc ccttccagct
    89041 tagtttcttc catataggcc agtacataga aggagttctg tttcaccagc gcaaccgccg
    89101 ttgatcctcg agtaataaac tcaccggtat agacgttgag gttggtcacc cagccatctg
    89161 ctggcgcgcg gatcaccgtg cgttcaagat ccagttttgc cagatcgcgg gtcgcctgcg
    89221 ctttcgctaa ctgatgcaga accgtttgta gtacgttgtt agcctagtcg atctcttcgc
    89281 gagacatcgc ctgcacaccg agacggttac gacgtccggc ctcctggcgt ttctcctgtg
    89341 ccagtacctg ataataagca acatcggctt gcgcttcctc aagcgccttt tgatagcgcg
    89401 gctggtcgat ggtgaacagt atctgtcctt ttttcaccag ctggttatca tgaacattca
    89461 cctgggtaat gagtccagaa acgtccggcg cgatcgcaat gacgtcagcg ctaaagcgcg
    89521 cgtcacgcgt ccagggggat tcggtgtaat agacccaggc attaaaaatt gcgatgaagg
    89581 ccagaatgac taatacgacc gtgatggccg tacgggagaa ttttcttatt agtgttttca
    89641 cttcaacctc aaacgaacag tcgcgatatc aaataaaaca agcagcaata gagcgcggtg
    89701 ttgaacaacg ccggatgcca gacaaagtcg tagatacctg ttggcacaag tacccggcgc
    89761 accagccaga aaatcgccag tgataaaagc aattcaaaaa atatcggtgg gaaggacagc
    89821 ccaaacacca cgataacggg aaacagactc atgttgacct tggttgtata gagagagcag
    89881 gcgttattat tttcagcatc tgtcgccgca gagaagggca tggaaagccg ggcgagagca
    89941 acattgctgt agattgatat ttaatatatt agcgtaactg ttatg
//
LOCUS       DAJTYD010000002        75509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTYD010000002 DAJTYD010000000
VERSION     DAJTYD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.002"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            110..322
                     /locus_tag="OO585_000095"
     CDS             110..322
                     /locus_tag="OO585_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6627816.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGAVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(346..1440)
                     /gene="ddlA"
                     /locus_tag="OO585_000096"
     CDS             complement(346..1440)
                     /gene="ddlA"
                     /locus_tag="OO585_000096"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6627817.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            1518..1675
                     /locus_tag="OO585_000097"
                     /pseudo
     CDS             1518..1675
                     /locus_tag="OO585_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1902..2162
                     /gene="iraP"
                     /locus_tag="OO585_000098"
     CDS             1902..2162
                     /gene="iraP"
                     /locus_tag="OO585_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6627818.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            2263..3678
                     /gene="phoA"
                     /locus_tag="OO585_000099"
     CDS             2263..3678
                     /gene="phoA"
                     /locus_tag="OO585_000099"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6627819.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            3797..4117
                     /gene="psiF"
                     /locus_tag="OO585_000100"
     CDS             3797..4117
                     /gene="psiF"
                     /locus_tag="OO585_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6627820.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            4219..5334
                     /gene="adrA"
                     /locus_tag="OO585_000101"
                     /gene_synonym="dgcC"
     CDS             4219..5334
                     /gene="adrA"
                     /locus_tag="OO585_000101"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6627821.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(5351..6160)
                     /gene="proC"
                     /locus_tag="OO585_000102"
     CDS             complement(5351..6160)
                     /gene="proC"
                     /locus_tag="OO585_000102"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6627822.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            6280..6738
                     /locus_tag="OO585_000103"
     CDS             6280..6738
                     /locus_tag="OO585_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6627823.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            6921..7445
                     /gene="aroL"
                     /locus_tag="OO585_000104"
     CDS             6921..7445
                     /gene="aroL"
                     /locus_tag="OO585_000104"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6627824.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            7495..7686
                     /gene="yaiA"
                     /locus_tag="OO585_000105"
     CDS             7495..7686
                     /gene="yaiA"
                     /locus_tag="OO585_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6627825.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            7944..8621
                     /locus_tag="OO585_000106"
     CDS             7944..8621
                     /locus_tag="OO585_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6627826.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            8693..8977
                     /gene="ppnP"
                     /locus_tag="OO585_000107"
     CDS             8693..8977
                     /gene="ppnP"
                     /locus_tag="OO585_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6627827.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            9125..9466
                     /locus_tag="OO585_000108"
                     /pseudo
     CDS             9125..9466
                     /locus_tag="OO585_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            9463..9546
                     /locus_tag="OO585_000109"
     CDS             9463..9546
                     /locus_tag="OO585_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6627828.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(9624..10535)
                     /gene="rdgC"
                     /locus_tag="OO585_000110"
     CDS             complement(9624..10535)
                     /gene="rdgC"
                     /locus_tag="OO585_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6627829.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            10660..11573
                     /gene="mak"
                     /locus_tag="OO585_000111"
                     /pseudo
     CDS             10660..11573
                     /gene="mak"
                     /locus_tag="OO585_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
     gene            complement(11672..12904)
                     /gene="araJ"
                     /locus_tag="OO585_000112"
     CDS             complement(11672..12904)
                     /gene="araJ"
                     /locus_tag="OO585_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6627830.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAGE
                     TTGVGYSLGVHYLLPDAA"
     gene            complement(13030..16173)
                     /gene="sbcC"
                     /locus_tag="OO585_000113"
     CDS             complement(13030..16173)
                     /gene="sbcC"
                     /locus_tag="OO585_000113"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6627831.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(16170..17372)
                     /gene="sbcD"
                     /locus_tag="OO585_000114"
     CDS             complement(16170..17372)
                     /gene="sbcD"
                     /locus_tag="OO585_000114"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6627832.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            17562..18251
                     /gene="phoB"
                     /locus_tag="OO585_000115"
     CDS             17562..18251
                     /gene="phoB"
                     /locus_tag="OO585_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6627833.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            18309..19604
                     /gene="phoR"
                     /locus_tag="OO585_000116"
     CDS             18309..19604
                     /gene="phoR"
                     /locus_tag="OO585_000116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6627834.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            20011..21330
                     /gene="brnQ"
                     /locus_tag="OO585_000117"
     CDS             20011..21330
                     /gene="brnQ"
                     /locus_tag="OO585_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6627835.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            21406..22779
                     /gene="proY"
                     /locus_tag="OO585_000118"
     CDS             21406..22779
                     /gene="proY"
                     /locus_tag="OO585_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6627836.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            22935..24657
                     /gene="malZ"
                     /locus_tag="OO585_000119"
                     /pseudo
     CDS             22935..24657
                     /gene="malZ"
                     /locus_tag="OO585_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(24662..25243)
                     /gene="acpH"
                     /locus_tag="OO585_000120"
     CDS             complement(24662..25243)
                     /gene="acpH"
                     /locus_tag="OO585_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6627837.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            25336..26406
                     /gene="queA"
                     /locus_tag="OO585_000121"
     CDS             25336..26406
                     /gene="queA"
                     /locus_tag="OO585_000121"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6627838.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTSVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26462..27589
                     /gene="tgt"
                     /locus_tag="OO585_000122"
     CDS             26462..27589
                     /gene="tgt"
                     /locus_tag="OO585_000122"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6627839.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            27612..27944
                     /gene="yajC"
                     /locus_tag="OO585_000123"
     CDS             27612..27944
                     /gene="yajC"
                     /locus_tag="OO585_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6627840.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            27972..29819
                     /gene="secD"
                     /locus_tag="OO585_000124"
     CDS             27972..29819
                     /gene="secD"
                     /locus_tag="OO585_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6627841.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            29830..30801
                     /gene="secF"
                     /locus_tag="OO585_000125"
     CDS             29830..30801
                     /gene="secF"
                     /locus_tag="OO585_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6627842.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGEDLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            30930..31277
                     /locus_tag="OO585_000126"
     CDS             30930..31277
                     /locus_tag="OO585_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6627843.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(31454..32338)
                     /gene="tsx"
                     /locus_tag="OO585_000127"
     CDS             complement(31454..32338)
                     /gene="tsx"
                     /locus_tag="OO585_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6627844.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTDLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(32637..33176)
                     /locus_tag="OO585_000128"
     CDS             complement(32637..33176)
                     /locus_tag="OO585_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6627845.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPRAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            33327..33776
                     /gene="nrdR"
                     /locus_tag="OO585_000129"
     CDS             33327..33776
                     /gene="nrdR"
                     /locus_tag="OO585_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6627846.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            33780..34883
                     /gene="ribD"
                     /locus_tag="OO585_000130"
     CDS             33780..34883
                     /gene="ribD"
                     /locus_tag="OO585_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6627847.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            34972..35442
                     /gene="ribE"
                     /locus_tag="OO585_000131"
     CDS             34972..35442
                     /gene="ribE"
                     /locus_tag="OO585_000131"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6627848.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            35462..35881
                     /gene="nusB"
                     /locus_tag="OO585_000132"
     CDS             35462..35881
                     /gene="nusB"
                     /locus_tag="OO585_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6627849.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            35959..36936
                     /gene="thiL"
                     /locus_tag="OO585_000133"
     CDS             35959..36936
                     /gene="thiL"
                     /locus_tag="OO585_000133"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6627850.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            36914..37432
                     /gene="pgpA"
                     /locus_tag="OO585_000134"
     CDS             36914..37432
                     /gene="pgpA"
                     /locus_tag="OO585_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6627851.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(37486..38460)
                     /gene="yajO"
                     /locus_tag="OO585_000135"
     CDS             complement(37486..38460)
                     /gene="yajO"
                     /locus_tag="OO585_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6627852.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(38515..40377)
                     /gene="dxs"
                     /locus_tag="OO585_000136"
     CDS             complement(38515..40377)
                     /gene="dxs"
                     /locus_tag="OO585_000136"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6627853.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(40402..41301)
                     /gene="ispA"
                     /locus_tag="OO585_000137"
     CDS             complement(40402..41301)
                     /gene="ispA"
                     /locus_tag="OO585_000137"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6627854.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(41301..41543)
                     /gene="xseB"
                     /locus_tag="OO585_000138"
     CDS             complement(41301..41543)
                     /gene="xseB"
                     /locus_tag="OO585_000138"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6627855.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            41749..43197
                     /gene="thiI"
                     /locus_tag="OO585_000139"
     CDS             41749..43197
                     /gene="thiI"
                     /locus_tag="OO585_000139"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6627856.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(43251..43841)
                     /gene="yajL"
                     /locus_tag="OO585_000140"
     CDS             complement(43251..43841)
                     /gene="yajL"
                     /locus_tag="OO585_000140"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6627857.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(43804..44715)
                     /gene="panE"
                     /locus_tag="OO585_000141"
     CDS             complement(43804..44715)
                     /gene="panE"
                     /locus_tag="OO585_000141"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6627858.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            44883..45374
                     /gene="yajQ"
                     /locus_tag="OO585_000142"
     CDS             44883..45374
                     /gene="yajQ"
                     /locus_tag="OO585_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6627859.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(45502..46866)
                     /locus_tag="OO585_000143"
     CDS             complement(45502..46866)
                     /locus_tag="OO585_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6627860.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(47015..47905)
                     /gene="cyoE"
                     /locus_tag="OO585_000144"
     CDS             complement(47015..47905)
                     /gene="cyoE"
                     /locus_tag="OO585_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6627861.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(47917..48246)
                     /locus_tag="OO585_000145"
     CDS             complement(47917..48246)
                     /locus_tag="OO585_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6627862.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIITILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(48246..48860)
                     /locus_tag="OO585_000146"
     CDS             complement(48246..48860)
                     /locus_tag="OO585_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6627863.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(48850..50841)
                     /gene="cyoB"
                     /locus_tag="OO585_000147"
     CDS             complement(48850..50841)
                     /gene="cyoB"
                     /locus_tag="OO585_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6627864.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(50863..51810)
                     /gene="cyoA"
                     /locus_tag="OO585_000148"
     CDS             complement(50863..51810)
                     /gene="cyoA"
                     /locus_tag="OO585_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6627865.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(52271..53746)
                     /gene="ampG"
                     /locus_tag="OO585_000149"
     CDS             complement(52271..53746)
                     /gene="ampG"
                     /locus_tag="OO585_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6627866.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(53790..54368)
                     /locus_tag="OO585_000150"
     CDS             complement(53790..54368)
                     /locus_tag="OO585_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6627867.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            54673..54990
                     /gene="bolA"
                     /locus_tag="OO585_000151"
     CDS             54673..54990
                     /gene="bolA"
                     /locus_tag="OO585_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6627868.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            55334..56632
                     /gene="tig"
                     /locus_tag="OO585_000152"
     CDS             55334..56632
                     /gene="tig"
                     /locus_tag="OO585_000152"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6627869.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            56877..57500
                     /gene="clpP"
                     /locus_tag="OO585_000153"
     CDS             56877..57500
                     /gene="clpP"
                     /locus_tag="OO585_000153"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6627870.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            57626..58900
                     /gene="clpX"
                     /locus_tag="OO585_000154"
     CDS             57626..58900
                     /gene="clpX"
                     /locus_tag="OO585_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6627871.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            59088..61442
                     /gene="lon"
                     /locus_tag="OO585_000155"
     CDS             59088..61442
                     /gene="lon"
                     /locus_tag="OO585_000155"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6627872.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            61651..61923
                     /gene="hupB"
                     /locus_tag="OO585_000156"
     CDS             61651..61923
                     /gene="hupB"
                     /locus_tag="OO585_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6627873.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            62115..63986
                     /gene="ppiD"
                     /locus_tag="OO585_000157"
     CDS             62115..63986
                     /gene="ppiD"
                     /locus_tag="OO585_000157"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6627874.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            64137..64508
                     /locus_tag="OO585_000158"
     CDS             64137..64508
                     /locus_tag="OO585_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6627875.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            64602..65000
                     /gene="fadM"
                     /locus_tag="OO585_000159"
     CDS             64602..65000
                     /gene="fadM"
                     /locus_tag="OO585_000159"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6627876.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(65052..65747)
                     /gene="queC"
                     /locus_tag="OO585_000160"
     CDS             complement(65052..65747)
                     /gene="queC"
                     /locus_tag="OO585_000160"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6627877.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMEKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(65812..67512)
                     /locus_tag="OO585_000161"
     CDS             complement(65812..67512)
                     /locus_tag="OO585_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6627878.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLALG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            67612..68394
                     /gene="cof"
                     /locus_tag="OO585_000162"
                     /pseudo
     CDS             67612..68394
                     /gene="cof"
                     /locus_tag="OO585_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
     gene            68583..69041
                     /gene="decR"
                     /locus_tag="OO585_000163"
     CDS             68583..69041
                     /gene="decR"
                     /locus_tag="OO585_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6627879.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            69071..70843
                     /locus_tag="OO585_000164"
     CDS             69071..70843
                     /locus_tag="OO585_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6627880.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASENIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            70836..72617
                     /locus_tag="OO585_000165"
     CDS             70836..72617
                     /locus_tag="OO585_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6627881.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            72798..73136
                     /gene="glnK"
                     /locus_tag="OO585_000166"
     CDS             72798..73136
                     /gene="glnK"
                     /locus_tag="OO585_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6627882.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            73166..74452
                     /gene="amtB"
                     /locus_tag="OO585_000167"
     CDS             73166..74452
                     /gene="amtB"
                     /locus_tag="OO585_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6627883.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(74501..75361)
                     /gene="tesB"
                     /locus_tag="OO585_000168"
     CDS             complement(74501..75361)
                     /gene="tesB"
                     /locus_tag="OO585_000168"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6627884.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
BASE COUNT        18245 a        18746 c        20285 g        18233 t
ORIGIN      
        1 aaatggcaac cttaagttaa atttattaac aattacgaaa atgttgtcta caccacagac
       61 ggttgttaca gaatatgcaa ggatgttgtt tttcgttaac ggagctgcca tgaatctgcc
      121 tgtaaaaatc cgccgtgact ggcactacta tgcgttcgcc attggcctta tattcattct
      181 taatggcgcg gtggggttac tgggatttga agcaaaaggt tggcagacct atgccgtcgg
      241 tctggtgacg tgggtgatta gtttctggct ggcggggttg attattcgtc gtcgcgatga
      301 agaaactgaa aacgcccaat aagaatatta tcgggcgtta aaatattaca ttgtggtttt
      361 caatgcgtta tccgcagcgt gacgctccag cgccagctca atcaaacgtg tgatcagatc
      421 ggtgtaaccc agaccgctgg cttgccacag cttcggatac atactgatgt tggtgaagcc
      481 aggcagtgtg ttgatctcgt tgatcaccac ttcgttctct ggggttaaaa acacgtctac
      541 acgcgccatg cctgcgcatc ccaacgtttg ataagcctga acggcaatcg cccggatctt
      601 atcgttgatt tctggcgcaa tggctgccgg aactaccact ttcgcgccat cttcgtcaat
      661 gtacttggtg tcgtaggcat agaagtcgct ggtgagtacg atctcgccac aggtgctggc
      721 ttgcgggttg tcgttgccca gaactgcgca ttcgatctca cgacctttga tcccttgctc
      781 aacgatcact ttatgatcga attcgaacgc cagatcgacg gcaattgcgt actgttcttc
      841 actggttact ttgctgacac caacagaaga gccctgatta gccggtttta caaacagtgg
      901 taacctcagt ttagactcca cttcggcaaa actaatgttg tgacgattgg cgcgcgtcag
      961 ggtaataaat ggcgcaatgt tcagcccggc atcgcgcagc agacgtttgg taacatcttt
     1021 atccatacag gctgctgaag ccagaacatc agaacctaca aacggtaaat tggcgacccg
     1081 cagcattccc tgcaaggaac catcttcgcc cagcgtaccg tggacaatcg ggaaaatgac
     1141 atccaccgtc ggtaacggct gaccgttttg cgcgtcgata agctgatgct catgtttact
     1201 tggcacctgc gcaaggctag tcgccgaagg gcgcaacgca atatgggcag gatcgtctgc
     1261 atttagcaga taattgctgg catcgctgac gtgccattgc ccttgtttat caatgcccag
     1321 cagcacaacg tcgaagcgac ttttatcaat ggcatcgaca atgttttttg ccgattgcag
     1381 agacacttca tgttccgctg atttaccacc aaaaacgatt cctacccgca gtttttccat
     1441 cttaaaaacc tatcccgtct aacacaaagt gcatacatta ccacgacaaa acgggggatt
     1501 cgcggccttc tgacagaatg ttgcaatctt ctgctgacaa agcgtgcaac gtaatggtga
     1561 agaaagtgcg ttattacaaa gatgtgcgcg agatcacaaa aatgatgaac gggaagctaa
     1621 tttattcctg gcttaaatgg ccatgcggtg agttttttct cttagttgta agtaaacgca
     1681 gagaatatat ttcataactt ttgtttataa taaaggttga taattaaagg cctgttcttt
     1741 gtgtggttaa ttatttaaat atgagaaacg tttcgctggt aatcaaacaa agaacatttg
     1801 cgcaaagtat ttcctttgtc ataaaaataa tacttccaga cactatgaag ttgtgaaaca
     1861 taatgttaac ttctccatac gttggataag gaaatacaga catgaaaaat ctcattgctg
     1921 agttgttatt aaagcttgcc caaaaagaag aagagtcgaa agaactgtgc gcgcaggtag
     1981 aagctttgga gattatcgtc actgcaatgc ttcgcaatat ggcacaaaat gaccaacagc
     2041 gcttgattga acaggtggag ggggctcttt acgaggtaaa gcctgatgcc agcattcctg
     2101 acgacgatac ggagctgctg cgcaattatg taaagaagtt attgaagcat cctcgtcagt
     2161 aaaaagttaa tcttttcaac agctgtcata aagttgtcac ggccgagact tatagtcgct
     2221 ttgtttttat tttttaatgt atttgtacat ggagaaaata aagtgaaaca aagcactatt
     2281 gcactggcac tcttaccgtt actgtttacc cctgtgacaa aagcccggac accagaaatg
     2341 cctgttctgg aaaaccgggc tgctcagggc gatattactg cacccggcgg tgctcgccgt
     2401 ttaacgggtg atcagactgc cgctctgcgt gattctctta gcgataaacc tgcaaaaaat
     2461 attattttgc tgattggcga tgggatgggg gattcggaaa ttacagccgc acgtaattat
     2521 gccgaaggtg cgggcggctt ttttaaaggt atcgatgcct taccgcttac cgggcaatac
     2581 actcactatg cgctgaataa aaaaaccggc aaaccggact acgtcaccga ttcggctgca
     2641 tcagcaaccg cctggtcaac cggtgtcaaa acctataacg gcgcgctggg cgtcgatatt
     2701 cacgaaaaag atcacccaac gattctggaa atggcaaaag ccgcaggtct ggcgaccggt
     2761 aacgtttcta ccgcagagtt gcaggatgcc acgcccgctg cgctggtggc acatgtgact
     2821 tcgcgcaaat gctacggtcc gagcgcgacc agtgaaaaat gtccgggtaa cgctctggaa
     2881 aaaggcggaa aaggatcgat taccgaacag ctgcttaatg ctcgtgccga cgttacgctt
     2941 ggcggtggcg caaaaacctt tgctgaaacg gcaaccgccg gtgaatggca gggaaaaacg
     3001 ctgcgtgaac aggcacaggc gcgtggttat cagttggtca gcgatgccgc ctcgctaaat
     3061 gcggtgacgg aagcgaatca gcaaaaacct ctgcttggac tgtttgctga cggcaatatg
     3121 ccagtgcgct ggctaggacc gaaagcaacg taccacggca atatcgataa gcccgcagtc
     3181 acctgtacgc ctaatccgca acgtaatgac agtgtaccaa ccctggcgca gatgaccgac
     3241 aaagccattg aattgttgag taaaaatgag aaaggatttt tcctgcaagt tgaaggtgcg
     3301 tcaatcgata aacaggatca cgctgcgaat ccttgtgggc aaattggcga gacggtcgat
     3361 ctcgatgaag ccgtacaacg ggcgctggaa ttcgctaaaa aggatggcaa cacgctggtc
     3421 atagtcaccg ctgatcacgc ccacgccagc cagattgtcg cgccggatac caaagctccg
     3481 ggcctcaccc aggcgctaaa taccaaagat ggcgcagtga tggtgatgag ctacgggaac
     3541 tccgaagagg attcacaaga acataccggc agtcagttgc gtattgcggc gtatggcccg
     3601 catgccgcca atgtcgttgg actgaccgac cagaccgatc tcttctacac catgaaagcc
     3661 gccctggggc tgaaataaaa ccgcgcccgg cagtgaattt tcgctgccgg gtggtttttt
     3721 tgctgttagc aaccagactt aatggcaaat ctcgggcgca tacgctcatg gttaaaacat
     3781 gaagagggat ggtgctatga aaattacatt actggttacc ttgcttttcg gtctggtttt
     3841 tttaaccaca gtcggcgctg ccgagagaac gttaacccca caacaacagc gtatgacctc
     3901 ctgtaatcag caggcgacgg cgcaggcgtt gaaaggggat gctcgtaaga cctacatgag
     3961 tgattgcctg aagaacagca agtctgcgcc tggtgaaaaa agtttgacgc cacagcagca
     4021 aaagatgcgc gaatgtaata atcaagcaac gcaacaatct ctgaaaggtg atgatcgtaa
     4081 taagtttatg agtgcctgcc tcaagaaagc cgcctgatac ctgatagtgc taacgggtga
     4141 gctacgaaag tggctcaccc gaaatttcat acttctgcct ttagccccgt ctctataatt
     4201 tgggaaaatt gtttctgaat gttcccaaaa ataatgaatg atgaaaactt tttcaaaaaa
     4261 gcggcggcgc atggggagga acctccttta actcctcaaa acgaacatca gcggtccggg
     4321 ctgcgcttcg cccgtcgcgt cagactaccc cgtgcggttg gcctggctgg catgttctta
     4381 ccgattgctt caacgctggt ttcgcacccg ccgccgggct ggtggtggct agtgttggtc
     4441 ggctgggcgt tcgtctggcc gcatttagcc tggcagatag cgagcagggc cgtcgatccg
     4501 cttagccggg aaatttacaa cttaaaaacc gatgcagtat tagcgggaat gtgggtaggc
     4561 gtaatgggcg taaacgtgct gccttccacc gctatgttga tgattatgtg tctgaatttg
     4621 atgggggcag gcggcccccg tctgtttgtc gcgggtctgg tgttgatggt ggtttcctgc
     4681 cttgtcaccc tcgagcttac gggcattacc gtgtcgttca atagtgcgcc gctggaatgg
     4741 tggctctccc ttcccattat tgtcgtttat cctctgctgt ttggctgggt cagctaccag
     4801 acggcaacca aactggcgga acataaacgc aggttgcagg tcatgagtac ccgcgacggc
     4861 atgacgggcg tgtataaccg acgtcattgg gaaactatgt tacgcaatga atttgataac
     4921 tgtcggcggc ataatcgcga tgcaacgtta ctgattatcg atatcgacca tttcaagagc
     4981 atcaacgata cctggggtca tgatgtgggc gatgaagcga ttgtggcgct tacccgacag
     5041 ttacaaatta ccctgcgcgg tagcgatgtg attggtcggt ttggcggcga tgagtttgca
     5101 gtaatcatgt ccggtacgcc agctgagagc gccattaccg ccatgttacg ggtgcgtgaa
     5161 gggctaaaca cgttacgctt gccgaatacg ccacaggtaa ctttacggat tagtgtgggg
     5221 gttgcgccgc tgaacccaca aatgagtcac tatcgtgagt ggttgaaatc ggcagatttg
     5281 gcgctttaca aagcaaagaa agccggacgt aaccgcaccg aagtggcggc ctgacgtcag
     5341 gcgaaagtca tcaggatttg ctgagttttt ctgatttttc catacacttc gtcatcgctt
     5401 cgatcactgc ggcacggaag cctttctctt ccagtacgcg taccgcttca atggtggtgc
     5461 ctcccggtga gcagaccata tctttcagtg cccccggatg ttctcccgtt tccagcacca
     5521 tttttgcgga acccattacc gcctgagcgg caaatttata cgcctgggcg cgtggcatcc
     5581 cgcccagcac ggcggcgtcg gccatcgctt cgataaacat aaatacgtag gctggcgaag
     5641 aaccgctcac accgactacc gggtggatca tcggctcagc aattacttcc gcttcgccaa
     5701 agcagcggaa aatattcagc acatcagcgg tatcttctgg ggttaccagc gcgtttggcg
     5761 ttacggaggt catcccggca ttaaccagcg cgggagtgtt cggcatggcg cggataattt
     5821 tccggtcatg gcccagcgcg cgggcaagct ggtcgagcgt gacacctgca gcaatagaaa
     5881 cgaccagaga gtctttattc aggctggagg tgatttcgct aagcacttta atcatgatgc
     5941 caggtttaac ggcagcaaaa atgatgtcgg cgatttgcgc cacttcttgc gccgattctg
     6001 cggcgttgat gccgaactgg tcatgcaggg cggcgacttt attcggggag ggggtgtata
     6061 cccagatttg ccctggaagc acctgaccgc tggcaatcag accgccgaga atggcttttc
     6121 ccatattgcc gcagccaata aaaccgattt tcttttccat tgcctcactc ctgccgtgaa
     6181 attcattgtt ttgataatcg ctggcagaag cataaacaga actatgccgg aaggcaaaag
     6241 cgcgacacaa tagaggatta cccaacaaag gatgacttta tgacaatttg ggtggatgct
     6301 gacgcgtgtc ccaatgtaat taaagagatt ttgtatcgcg cggcggaacg tatgcagatg
     6361 ccgctggtac tggtagcaaa ccagagttta cgcgtgccgc catcgcgatt tattcgtacg
     6421 ctgcgcgtcg cggcaggttt cgacgttgcc gataacgaaa ttgtccggca gtgtgaagcg
     6481 ggcgatttgg tgatcaccgc agatatacct ttggctgctg aagccatcga gaaaggcgca
     6541 gcggcgctta atccgcgcgg cgaacgttac acgccagcga ccattcgtga gcgcctgacg
     6601 atgcgcgatt ttatggatac cttacgtgcc agtgggatcc agaccggcgg accagatagc
     6661 ctttcacaac gtgaccgcca ggcctttgcc gcggagctgg agaagtggtg gctggaagtg
     6721 caacgtagtc gtggctaaat gtaatttatt atttacactt cattcttgaa tatttattgg
     6781 tatagtaagg gatgtattga gattttcact ttaagtggaa ttttttcttt acaatcgaaa
     6841 ttgtactagt ttgatggaat gatcgctatt ctcatgacac cggctttcgc cgcattgcga
     6901 cctattgggg aaaacccacg atgacacaac ctctttttct gatcgggcct cggggctgtg
     6961 gtaaaacaac ggtcggaatg gcccttgccg attcgcttaa ccgtcggttt gtcgataccg
     7021 atcagtggtt gcaatcacag ctcaatatga cggtcgcgga gatcgtcgaa agggaagagt
     7081 gggcgggatt tcgcgccaga gaaacggcgg cgctggaagc ggtaactgcg ccatccaccg
     7141 ttatcgctac aggcggcggc attattctga cggaatttaa tcgtcacttc atgcaaaata
     7201 acgggatcgt ggtttatttg tgtgcgccag tatcagtcct ggttaaccga ctgcaagctg
     7261 caccggaaga agatttacgg ccaaccttaa cgggaaaacc gctgagcgaa gaagttcagg
     7321 aagtgctgga agaacgcgat gcgctatatc gcgaagttgc gcatattatc atcgacgcaa
     7381 caaacgaacc cagccaggtg atttctgaaa ttcgtagcgc cctggcacag acgatcaatt
     7441 gttgatttta gagcgcctat acttaacgtt cattccgtga aataaggaag aacgatgcca
     7501 acgaaaccgc cttatcctcg tgaagcatat atagtgacga ttgaaaaagg aaagccagga
     7561 cagacggtaa cctggtacca actcagagcc gatcatccta aaccagactc gttgatcagt
     7621 gaacatccga ccgctcagga agcgatggat gcgaaaaaac gctatgagga ccctgacaaa
     7681 gagtgaccgc atcagactgc tcggaaggga ttctgagtgc cactacaagg gatctgcgtc
     7741 acatttttca taattcatgt ttttctaata attagaatat taaacaataa caatccatta
     7801 ctggaatcat ttggaatctt tacattatgc cgtgcacgtc tgctgctacg ctttttgtca
     7861 tttgtagcac aagtaagtgt cagcagtggt gcttcacact tgcccggtaa ttaacgacga
     7921 aagaaaagta aggtggatga acaatgagtg cgtcgttggc gatcctgacc atcggcattg
     7981 tacctatgca ggaggttttg ccgctcctga cggaatacat tgacgaagat aatatttccc
     8041 atcatagcct gctggggaag ttaagtcgtg aagaagtgat ggcggagtac gcgccagaag
     8101 caggcgaaga caccattctc acattattaa atgacaacca gctggcccat gtttcgcgtc
     8161 gtaaagtgga gcgagacctg caaggtgtgg ttgaagtgct cgataatcag ggttatgacg
     8221 tcattttatt aatgagtaca gcaaacatta gtagtatgac tgcgcgtaat acgatctttc
     8281 ttgagccgtc gcgaatattg cctccactgg tttcctctat tgttgaagat catcaggtgg
     8341 gggttatcgt tccggttgag gagatgctgc ccgttcaggc gcaaaaatgg caaattttgc
     8401 agaaatcgcc ggtattttca ttgggtaacc ccattcatga ttcagaacaa aaaatcattg
     8461 atgcagggaa agaattactg gcaaaagggg ctgatgtcat catgctggat tgtttaggat
     8521 ttcaccaacg tcatcgcgat ttactgcaaa aacagctcga tgttcctgtc ttgctgtcta
     8581 acgtattgat tgcacggctg gctgcggaat tactggtgta attttgcgtg acagccagcg
     8641 cctctggccc ctatagtgaa gtagatgttc aactaccaga cagggccagt ttatgcttca
     8701 aagtaatgag tacttttccg gcaaagtgaa atcaatcggc ttttccagca gcagcactgg
     8761 tcgcgccagc gtgggtgtta tggttgaagg cgaatacacc ttcagcaccg ctgagccgga
     8821 agagatgacg gtaatcagtg gcgcgctgaa tgtgttactg cctgatgcga ccgactggca
     8881 ggtgtatgaa gccggttcgg tgtttaatgt tcccggtcac agtgagtttc atctgcaagt
     8941 tgccgaaccc acctcttatc tgtgccgcta tctgtaattc ctcgccttcc ccttgaacgg
     9001 gagggcattt ttctgaaata tcctctcttt aacccataat aatatttcct ttgctgcgat
     9061 tttttcaatt tccgatatat tcataattca tcaaggttga tataaatatc agtgaagatc
     9121 tccagatatt gttgcggaac tggctacgat aaaagataaa tcagatgatg aatggtggcg
     9181 tgcattgctg caaagtcgca atgatcattt gcgccaaacg gcattacgca acgcacatac
     9241 cccggcgtcg ttgttaacga cattgaccga gtctcggcat cggtcgctag ctatgaataa
     9301 tccacagctc gctgccgatg tgaaaacgac gtggttaaaa gaggatccat cattactctt
     9361 atttgtcgaa caacccgatc tttcgctgtt acgggattta gtgaaaaccg gggcaatgcg
     9421 gaaaattcgc agtgaagcgc gtcactggct tgaggaaaaa caatgacaca acgtccatgg
     9481 agcaagttac agcataaaac gcataatatt gctgcgctaa aaattattgt ccgccgtagc
     9541 gaataattat atccctggtg tggcttcgta cgccggataa gacgcggcag gcgtcgcatc
     9601 cggcattaaa ggaaaatcag caattaacgt tgtgcttcgc cacctaatcc ttcaatcagg
     9661 ttttgaatta acgctgccag ttcaccagtc atcaggatga aatcggcatc aaaacgctgg
     9721 gcgaaatctt cacggtcgat atcttcgttt tgatcgcgca gctcgtcgca aaacttcaga
     9781 cgcttgagcg aaccatcgtc gcacatcaca aactgaatgc gctgctgcca gtcgagcgcc
     9841 agtttagtca ccacttttcc ggcttcgatg tgattggtga tctcttcgct ggtcagatct
     9901 tgtttcttcg cgcggatcac gccgccatct tccagcaacg atttcagctc ggcttcatca
     9961 agcagctgga agccctgtgc cgcgctaccg gagcgaaccc attcggtcag cgtcagttca
    10021 atcgggtttt ccatgctcaa tggtaccaca ggtaacgacc ccaggctttt acgcagcagt
    10081 gccagcgtat cttccgcttt tttggcactg gcgcagtcca ccataatcaa accgttaacc
    10141 gtgtcgatcc acatcattgt ctggctaaaa cggctgaaag cacgcggcaa cagagagtgc
    10201 agcacttcgt ctttcagcga atctttttcg gttttcttca gcttacgcgc ctgttccgct
    10261 tccagtttgg cgattttcgc ttccagcgcc tgcttaatca ccggagacgg gaggattttt
    10321 tcttctttgc gcgcgcagat aacaatttga ccattggcaa cgtgcgttaa cgcatcgctg
    10381 tgcgatccca tcggaggaac ccagcccatc ttcgccatgt cctggctgcc gcatggggta
    10441 aatgccatcg aggctagctg tttttccatc tcttctgcac gcagcgaaat ctcgcggcta
    10501 agacggtaaa ccattaaatt tttgaaccac agcatgataa tttccacggc cttgttgtta
    10561 aatttagcgg gcatgataac gaattgtcgg cggccttgca ttgccaatcc ggttgtccgt
    10621 ctctacgcta ttgatattga aaaaaataag gagagtaccg tgcgtatagg tatcgattta
    10681 ggcggcacca aaactgaagt gattgcactg ggcgatgcag gggagcagtt gtaccgccat
    10741 cgtctgccca cgccgcgtga tgattaccgg cagactattg aaacgatcgc cacgttggtt
    10801 gatatggcgg agcaggcgac ggggcagcgc ggaacggtag gtatgggcat tcctggctca
    10861 atttcgcctt acaccggtgt ggtgaagaat gccaattcaa cctggctcaa cggtcagcca
    10921 ttcgataaag acttaagcgc gaggttgcag cgggaagtgc ggctggcaaa tgacgctaac
    10981 tgtctggcgg tttcagaagc agtagatggc gcggcagcgg gagcgcagac ggtatttgcc
    11041 gtgattatcg gcacgggatg cggcgcgggc gtggcattca atgggcgggc gcatatcagc
    11101 ggcaatggca cggcaggtga gtggggacac aatccgctac cgtggatgga cgaagacgaa
    11161 ctgcgttatc gcgaggaagt cccttgttat tgcggtaaac aaggttgtat tgaaaccttt
    11221 atttcgggca cgggattcgc gatggattat cgtcgtttga gcggacatgc gctgaaaggc
    11281 agtgaaatta tccgcctggt tgaagaaagc gatccggtag cggaactggc attgcgtcgc
    11341 tacgagctgc ggctggcaaa atcgctggca catgtcgtga atattctcga tccggatgtg
    11401 attgtcctgg gggggggggg gggatgagca atgtagaccg tttatatcaa acggttccgc
    11461 agttgattaa acaatttgtc ttcggcggcg aatgtgaaac gccggtgcgt aaggcgaagc
    11521 acggtgattc cagcggcgta cgcggcgctg cgtggttatg gccacaagag taaaaaacgt
    11581 aggcaattgg cgcatcatgc ctgatgcgac gcttgccgcg tcttatcagg cctgcaaaat
    11641 gtgccagaac cgcgtagggc ggataaggcg ttcacgccgc atccggcaat aagtaatgaa
    11701 caccgagact ataacctacc ccagtggttt cgccagcaag caccggagta tccgccgctt
    11761 gctggcgctt atagcgacca tacagcagca acgacgacat cgcagcaaac gaaagcaggg
    11821 cggcaggcag cgccacgtaa ttatatgcca gccccagcgt cagcatcata ccaccgcaat
    11881 atgcgccgac ggcgctaccg aggttaaacg ctatttgccc acctgcggca cctaataact
    11941 ctccgccttt ggcgttttgt agtaacaata tttgtagcgg tgctgaaagg gcaaataatc
    12001 ccgcgcaaca aataaaagca aaaataagcg acgttgtttt catgccgccg cagaaaaaga
    12061 acatcagcag tgccagtaca attataaagt cagtcactgc tgcaatgcgc agtggtgaat
    12121 aacgtcctga aatcctgcca cttagcatat ttcccagcac catccctagc ccaactaaca
    12181 tcataataaa ggtcatcgcc gtttccgaaa aaccggaaat aaacatcatg tatggcttta
    12241 cgtagctgaa ccaggcaaac acacctgcgt tgccaaacat ggtggcggcg aaaattaacc
    12301 acggggccgg gctgcgcaaa aagtgaaatt gttcgcgcag ctttcctttc gcctcgtcgc
    12361 gaatatccgg cacccaaaaa tagaccgatg ccatcaccgc aatattaaaa acagcgatca
    12421 ataaaaaggt gtaacgccag ctaaattcct gacttaaata cgttcccagc ggaatgccca
    12481 gcaaattggc gactgtcatc ccggaaacca tccccgccac ggcggcggtg acttttccgg
    12541 gtttgataat ttttgataac acgatcgcgc cgacgccaaa aaatgcgcca tgcggaaagc
    12601 cggataccag ccgaccaatg gcgagcatca ggtaagacga agagagcgtg aacatggcgt
    12661 tgccaatgac gcacaacgcc accagaaaca acaagatatg tttgagtgag tagcggctgg
    12721 aaaagagtgc gatgattggc gcaccgacca ccactcccag tgcataatac gagatcatat
    12781 gcccggcagc aggaatcgaa attcctacgt tatgcgccag ctccgtgagc acgcccataa
    12841 tgccaaattc ggccatcccc aaaccaaacg tgcccagagc caaagataaa atgacttttt
    12901 tcataccacc atcacaacca ccagaacggc taccaatcac cgtagccaat aaagtgatag
    12961 cttgcaggat aacgaccagc aaggccaaga gataattcgc ccctctgtat tcattatcct
    13021 gctgaatagt tatttcactg caaacgtact ttccagtttg ctgtagccca ggccgttgat
    13081 ctttttcact ttgatctgca ccggaatacg ctctttcatc gcttctacgt ggctaatcac
    13141 accgatggtt ttgccactgg cgttcagggc atccagcgca tcaagggcgg tatccagcgt
    13201 ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc gagtcaatac gtgttttatg
    13261 gctgaccaga tccgaaagcg ccagcgccag cgccagacta acgaggaaac tttcgccgcc
    13321 ggaaagggta cgggtatcgc gtaccgcatc tgcctgccag gtatcaacaa cctcgacttc
    13381 cagcgcctcg ctggctttgc gctgtaacag atagcgcccg tgcagccggg taagttgctg
    13441 attagcgaga tggactaaat tatccagcgt cagcccctgg gcaaacttgc ggaatttatc
    13501 gccctctttg gaacctatta gcgaattcag atatccccag tcctcaacct gctgcgtcat
    13561 ttgagcaatt tgctgcatta aggtttgttg ttgctgacgg ttatctgcat cctgcttcag
    13621 ctgctggcga atctcgcctt gactcgtggt gttttcacgc aacttttggt gagtttgcgc
    13681 taactcttgc tgaatctgct ccaccgtcac agtgagagcc aacccgtcag gtcggtgttg
    13741 ttgatgctgt gccagcgttt ctgctgtctg agtgaccagc gtttgcgcct gacggcgctg
    13801 gttttccaga ttctgcttga gctgttccag ctgcgttagt gtttgttcat ccattagcgc
    13861 cgcaaggaac gcctgctgat cgtcaaagac gctggcctgt agcgcggtgt caaactgcgc
    13921 ctgggctttt tgcagacttt gcgccgccag aacatcctgt tgctgtaacg tctgctgctg
    13981 gctgtgtaat gcgagacatt gttcatgaac ctgccgccag ttatccagcg ccacagtttc
    14041 ttcgctgtgc gggagatcat cactttgcgg caacgtttcc agaatcggcg tcagctgctg
    14101 aatacggttt tgcagcgtgg ttaattcgtt ctggcgttgc tgccagctct gcgcttcttg
    14161 ctgacgtgtc gccaaccagc tctcttcttc atcttcctgt ggcaatgtca gtgcataacc
    14221 cgccaatgcc gttaagagct gctgctggcg ttgttcaatt tgctgttgat actggataat
    14281 ttgctgatta tgcgcggcaa tctgcccttg taattcatgt cgttggctga gtaaccgcag
    14341 ctggcgtttg tgctcatctt gtgcatccag ccacggttga atatcgtcct gtggctgcaa
    14401 ggtgatattg aggctggccg tgacggcttg ccattgttga gtaagtgctt gctcatcttg
    14461 tcggaggctt tgcgcttcgt tttcatcgcg ctgaagctgc ttcgttaatg catccagttg
    14521 cccgcgcagc gccgcacctt cttcgccgag ctttttaact tcgttttcca gcgccagtaa
    14581 tcgagactga ttaacaccag gctccagcgc ctgatacgcc ttgaccgccg ggtggctggt
    14641 ggaaccacaa agtggacaag gctgacccgc ctgtaactgt gcacgctggg cttccagcgt
    14701 tttgatgcgc gcttcctgct cgcaaatggt tttcacatcg gcaagttgct gcgtcttttc
    14761 tttataacgc tggcgcattt cgttaagtgc gacattacgt tgcgtctgtt ccagagatcc
    14821 attctggata gtgaccatta actgcgccag acgtttttgt tggggaacaa tctgcccatg
    14881 cagcgcgacc aggcgctgac gcagtgggcg ttgctcagca tgttgcgcca gggcggtagc
    14941 aacttcatcg gcggttaacg tcaacgtgat cgccgcaagc gcattaagtt tttgctcagc
    15001 atgggttaac tgttgctgcc attgccgcag atgctcgcga tcgctggttt gttgggagaa
    15061 ctgcgcacgc caacccgcca gttcgttgtt ccactgacgg aagcggtcgt gttcctgtaa
    15121 ccaggtattc aggctttgtt gctgctgctg taattctgct gactgcttcg ccgcgtggtg
    15181 gcgaatgctc gcgcgaagcg ccattgtgct ctgtaagcga gtatttactt cttcaatctg
    15241 ctggcgagta tgcgccagcg ccgcgctgtg ttctgcgatg cgttcccagt gcggacgaag
    15301 atttcgtgcc ggttgtgcca gactgagcgc cgccagttga ggttgcgctt tttcttcttc
    15361 ggctaacgcc tgttgcaagg cctgctgacg gcggctggct tcttgctgca attcgtccag
    15421 acgcgttaac cagtttagtg attgttgttc ttgctgctgc gcggtaagta actgtttttc
    15481 ttcgtcagta agtacctgca aactcgctgt cagcgattgc acttgttccg gcgtgagcaa
    15541 cgcgacgccg ctggcctgcg cttgcagctt ctccagctct gtgcgggccg atttgtgctg
    15601 ctcaaaaacc atcgccgaga tttgcccgta gatttcagtg ccggttaact cctcgagcaa
    15661 ttccgcgcgt tctttgggtt tggcattcag gaaggcagca aattgcccct gcgaaagcag
    15721 catcgaacgg gtgaagcgcc cgtaatccag cccggttaac gtcgctgtca gttccagctt
    15781 atctttcact ttgtcggcga gaattttgcc gtcggcgcag cgcgccagct ctacgcgtgg
    15841 cacctgcaaa ttaccgtcgg gttggttacg cgcccgattc tggctccaga atgcacggta
    15901 cgcttcacct ttcacttcaa actccacctc cgccagacat tcggcggtat cgcgggtcat
    15961 gagatcattt tgcgattgtg aaacgttaga gagacgcgga gtttcgtgat acagcgccag
    16021 acaaatggcg tccagcaggg tggttttccc cgcaccggtt gggccggtaa tggcaaacag
    16081 cccgttgctg gcgaacggct cgcgggtgaa atcaatcttc cattcgcctt ttaatgagtt
    16141 caggtttttc aggcgcaggc tgagaatttt catgcttcgt gttctccggc gagggtatgc
    16201 aacgtcgtgg tgaaaagatg ctgcagacgt tgctgctgcg attcatccag ttcttccagt
    16261 gccagacggc gattgaacac ctcttcgacg ctgagttcgc tgagggtttc acgctgttgg
    16321 ctggctaaca cacgctcgcg ctgttcacga ctccgacgta ccagcaatac ttcgacaggc
    16381 aatgattcgg ctaatgcctg gattttgcgc tgaatatcat gcagatactc atcagtagtg
    16441 atttcgatat ccagccagac aggtggctcc tgcgatacat cgcgccactg ttccagctgt
    16501 gcggtaatcg acgccagatc gcctttcagc accgccatgg gttgagttac cggtacgttc
    16561 aggttttcca cgctctctaa tttgccgttt gaaaatgtca ccagatggac atatttactc
    16621 ttaccgcatt catcaaaact cagtggaatg ggggaaccgc aatagcgaac atgttccatg
    16681 ccgccaataa tctgtgcgcg gtgaatatgc ccgagcgcga tgtagtcggc tggtggaaag
    16741 ttttgtgccg gaaacgcgtc cagcgtgcca atataaatgt cacgcacggc gtcactttta
    16801 ctggcaccca cggtcgttaa atgtcccgtg gcgatgatgg gcagaggctg atcgccgcgc
    16861 agtttgcagg catcggcata gtgttgttgg taataatcgg taatcgctgc cagtaaatgc
    16921 tgctgttttt caataccgtt aagccccgcc tggctggaaa taatgtcacg cgggcgtaaa
    16981 aacggaatgg ggcacagcac tgcgcctggc gtcccgtcgc gacgaggcaa gatttgcggc
    17041 gcatgtccgg cgctggcgac cacggttgta ttgaggaacg ccatgatatc gcgcgattca
    17101 ttcagcgtgg cgaccgagtc atggtttcct gccagtacca ccagatgaca gccagtttgc
    17161 tgtaaattga caacaaaacg gttgtataac gtgcgggcgt aactgggcgg cgagccggta
    17221 tcgaaaacat caccagcaac aataatcgca tccacctgat gggtttgtgc tgtctccagc
    17281 agccagtcaa gaaaagcctg atgttcagct tcgcggcttt tactgtagaa gttctggccg
    17341 agatgccagt ctgaggtgtg aaggatgcgc ataacggttc cctggcgaaa aagcatggtc
    17401 gcgattatac ccaaacagat gtgccatttg cttttttctg cgccacggaa atcaataacc
    17461 tgaagatatg tgcgacgagc ttttcataaa tctgtcataa atctgacgca taatgacgtc
    17521 gcattaatga tcgcaaccta tttattacaa cagggcaaat catggcgaga cgtattctgg
    17581 tcgtagaaga tgaagctcca attcgcgaaa tggtctgctt cgtgctcgaa caaaatggct
    17641 ttcagccggt cgaagcggaa gattatgaca gtgctgtgaa tcaactgaat gaaccctggc
    17701 cggatttaat tctccttgac tggatgttac ctggcggctc cggtatccag ttcatcaaac
    17761 acctcaagcg cgagtcgatg acccgggata ttccagtggt gatgttgacc gccagagggg
    17821 aagaagaaga tcgcgtgcgc ggccttgaaa ccggcgcgga tgactatatc accaagccgt
    17881 tttcgccgaa ggagctggtg gcgcgaatca aagcggtaat gcgccgtatt tcgccaatgg
    17941 cggtggaaga ggtgattgag atgcagggat taagtctcga cccgacatct caccgagtga
    18001 tggcgggcga agagccgctg gagatggggc cgacagaatt taaactgctg cactttttta
    18061 tgacgcatcc tgagcgcgtg tacagccgcg agcagctgtt aaaccacgtc tggggaacta
    18121 acgtgtatgt ggaagaccgc acggtcgatg tccacattcg tcgcctgcgt aaagcactgg
    18181 agcccggcgg gcatgaccgc atggtgcaga ccgtgcgcgg tacaggatat cgtttttcaa
    18241 cccgctttta acgccttgct catcggacgc agagcagggc ttatgatttc ttaactggag
    18301 tatcttacgt gctggaacgg ctgtcgtgga aaaggctggt gctggagctg ctactttgtt
    18361 gcctcccggc tttcatcctg ggtgcatttt ttggttacct gccctggttt ttgctggcat
    18421 cggtaacagg actgcttatc tggcatttct ggaatttatt gcgcctttca tggtggttgt
    18481 gggtggatcg cagtatgacc ccgccaccgg ggcgtggtag ctgggaaccg ctactatacg
    18541 gcttacacca gatgcagctg cgaaataaaa aacgccgccg tgaactgggc aatctgatta
    18601 aacgctttcg tagcggcgcg gagtcgctgc ccgacgcggt ggtgctgacc acggaagagg
    18661 gcggtatttt ctggtgtaac ggtctggcgc aacaaattct tggtttgcgc tggccggaag
    18721 ataacgggca gaacatcctt aacctactgc gttacccgga gtttacgcaa tatctgaaaa
    18781 cgcgtgattt ttctcgcccg ctcaatctgg tgctcaacac cgggcggcat ctggaaattc
    18841 gcgtcatgcc ttatacccac aaacagttgc tgatggtggc gcgtgatgtc acgcaaatgc
    18901 atcaactgga aggggcgcgg cgtaactttt ttgccaacgt gagccatgag ttacgtacgc
    18961 cattgaccgt gttacagggt tacctggaga tgatggatga gcagccgctg gaaggcgcgg
    19021 tacgcgaaaa agcgttgcac accatgcgcg agcagaccca gcggatggaa gggctggtga
    19081 agcagttgct aacgctgtcg aaaattgaag ccgcgccgac gcaattgctc aatgaaaagg
    19141 ttgatgtgcc gatgatgctg cgcgttgttg agcgcgaggc tcagactctg agtcagaaaa
    19201 aacagacatt tacctttgag atagataacg gcctcaaagt gtctggcaac gaagatcagc
    19261 tacgcagtgc gatttcgaac ctggtctata acgccgtgaa tcatactcca gaaggcacgc
    19321 atatcaccgt acgctggcag cgagtgccgc acggtgccga atttagcgtt gaagataacg
    19381 gaccgggcat tgcaccggag catattccgc gcctgaccga gcgtttttat cgcgttgata
    19441 aagcgcgttc ccggcaaacc ggcggtagcg gattagggtt agcgatcgtg aaacatgctg
    19501 tgaatcatca cgaaagtcgc ctgaatattg agagtacagt aggaaaagga acacgtttca
    19561 gttttgttat cccggaacgt ttaattgcca aaaacagcga ttaatccgcc tttgtcatct
    19621 tttattgcca taagccagtc gatgctggct tattttcttt gcagtcaaaa tacgggcgtt
    19681 agattttaca acggttggtg attttttgtt cgcatgatta gccatgtctt tttcacggaa
    19741 atagtgtttt atactggttg gtgatttctt atcgctatat acctctggtt tttagatccc
    19801 tccttgcttt aaaacgttat aagcgtttaa attgcgcttc aggtgctgtc atactgactg
    19861 cattaacgcg gtaaatcgaa aaactattct tcgccgcgcc aggttgggag tatttcccgc
    19921 taaaattgtt taaatatacc gctgtatcat ccccagggat tggcacaaaa atttaacgtt
    19981 acaacaccac atccacaggc agtatgattt atgacccatc aattaagatc gcgcgatatc
    20041 atcgctctgg gctttatgac atttgcgttg ttcgtcggcg caggtaacat tattttccct
    20101 ccaatggtcg gcttgcaggc aggcgaacac gtctggactg cggcattcgg cttcctcatt
    20161 actgccgttg gcctgccggt attaacggta gtggcgctgg caaaagttgg cggcggtgtt
    20221 gacagcctca gcacgccaat cggtaaagtc gctggcgtgc tgctggcaac ggtttgttac
    20281 ttggcggtgg ggccgctttt cgctacgccg cgtacagcta ccgtttcctt tgaagtgggg
    20341 attgcgccgc tgacgggtga ttccgcgctg ccgctgttta tctacagcct ggtctatttc
    20401 gctatcgtta ttctggtttc gctctatccg ggcaagctgc tggataccgt gggcaacttc
    20461 cttgcgccgc tgaaaattat cgcgctggtc atcctgtctg ttgccgctat tgtctggccg
    20521 gcgggttcta tcagcacggc gactgaggct tatcaaaacg ctgcgttttc taacggcttc
    20581 gttaacggct atctgaccat ggatacgctg ggcgcaatgg tgtttggtat cgttattgtt
    20641 aacgcggcgc gttctcgtgg cgttaccgaa gcgcgtctgc tgacccgtta taccgtctgg
    20701 gctggcctga tggcgggtgt tggtctgact ctgctgtacc tggcgctgtt ccgtctgggt
    20761 tcagacagcg cgtcgctggt cgatcagtct gcaaacggcg ctgctattct gcatgcttac
    20821 gttcagcaca catttggcgg cggcggtagc ttcctgctgg cggcgttaat cttcatcgcc
    20881 tgcctggtaa cggcagttgg cctgacctgt gcttgtgcag aattctttgc ccagtacgta
    20941 ccgctctctt atcgtacgct ggtgtttatc ctcggcggct tctcgatggt ggtgtctaac
    21001 ctcggcttaa gccagctgat ccagatctcc gtaccggtgc tgaccgctat ttatccgccg
    21061 tgtatcgcac tggttgtatt aagttttaca cgctcatggt ggcataattc gtcccgcgtg
    21121 attgccccgc cgatgtttat cagcctgctt tttggtattc tcgacgggat caaagcatct
    21181 gcattcagcg atatcttacc gtcctgggcg cagcgtttac cgctggccga acaaggcctg
    21241 gcgtggttaa tgccaacagt ggtgatggtg gttctggcca ttatctggga tcgcgcggca
    21301 ggtcgtcagg tgacctccag cgctcactaa atcactgaac atttgtttta accacggggc
    21361 tgcgatgccc cgtggttttt tattgtgttg atgggttagg aattgatgga aagtaagaac
    21421 aagctaaagc gtgggctaag tacccgccac atacgcttta tggcactggg ttcagcaatt
    21481 ggcaccgggc tgttttacgg ttcggcagac gccatcaaaa tggccggtcc gagcgtgttg
    21541 ttggcctata ttatcggtgg tatcgcggcg tatatcatta tgcgtgcgct gggggaaatg
    21601 tcggtacata acccggccgc cagctctttc tcgcgttatg cgcaggaaaa cctcggcccg
    21661 ctggcaggtt acattaccgg ctggacctac tgctttgaaa tcctaattgt cgccatcgcc
    21721 gatgtgaccg cttttggtat ctatatgggt gtctggttcc cgacggtgcc gcactggatt
    21781 tgggtactga gcgtggtgct gatcatttgc gccgtaaacc tgatgagcgt gaaggtattc
    21841 ggtgagctgg aattctggtt ctcgttcttt aaagtcgcca ccatcatcat catgattgtc
    21901 gccggtttcg gcatcatcat ctgggggatt ggcaacggcg ggcaaccgac cggtattcat
    21961 aacctgtgga gcaacggcgg cttcttcagt aacggctggc ttggcatggt aatgtcgttg
    22021 caaatggtga tgtttgctta cggtgggatc gaaattatcg ggattaccgc cggtgaagcg
    22081 aaagatcctg agaaatcgat tccgcgtgcg attaactccg tgccgatgcg tattctggtg
    22141 ttctacgtcg gtacgctgtt cgtcattatg tctatctacc cgtggaatca ggttggcact
    22201 gccggtagcc cgttcgtgct gacgttccag catatgggca ttacctttgc cgccagcatt
    22261 cttaactttg ttgtgctgac cgcttcgctg tcggcaatta acagtgacgt atttggcgta
    22321 ggccgtatgc tccacggtat ggcagagcag ggcagcgcac cgaaaatttt cagcaaaacg
    22381 tcgcgtcgcg gtattccgtg ggttacggtg ctggtgatga ctaccgcgct gctgtttgcg
    22441 gtgtatctga actacatcat gccggaaaac gtcttcctgg tgatcgcttc gctggcaacc
    22501 ttcgccacgg tatgggtgtg gattatgatc ctgctgtcgc aaatcgcctt ccgtcgccgt
    22561 ttgccgccag aagaagttaa ggcgctgaaa tttaaagtgc cgggtggggt agcaacgacc
    22621 atcggcgggc tgattttcct gctctttatt atcgggttga ttggttatca cccggatacg
    22681 cgtatctcgc tgtatgtcgg tttcgcgtgg attgttgtgc tgttgattgg ctggatgttt
    22741 aagcgtcgcc acgatcgtca gctggctgaa aaccagtaat ccttgcgttc tgtaagccgg
    22801 ttcaggagga aatcctgatc cggctattcc gaaagttatc cgcccccgtc ctcctccccc
    22861 aaatatcctt cagatgatga gtgatcctgc attaggctat ggcaaggtga tcagattttc
    22921 atcacagggg aattatgatg ttaaatgcat ggcacctgcc ggtgccccca tttgttaaac
    22981 aaagcaaaga tcaactgctc attacactgt ggctgacggg cgaagaccca ccgcagcgca
    23041 ttatgctgcg tacagaacac gataacgaag aaatgtcagt accaatgcat aagcagcgca
    23101 gtcagccgca gccaggcgtc accgcagggc ttcagccgaa tgccgccggc acgactggag
    23161 cagtttgccg tcgatgtacc ggatatcggc ccacaatggg ctgcggatca gattttttat
    23221 cagatcttcc ctgatcgttt tgcgcgtagt cttcctcgtg aagctgaaca ggatcatgtc
    23281 tattaccatc atgcagccgg acaagagatc atcttgcgtg actgggatga accggtcacg
    23341 gcgcaggcgg gcggatcaac gttctatggc ggcgatctgg acgggataag cgaaaaactg
    23401 ccgtatctga aaaagcttgg cgtgacagcg ctgtatctca atccggtgtt taaagctccc
    23461 agcgtacatc aatacgatac cgaggattat cgccatgtcg atccgcagtt tggcggtgat
    23521 ggggcgttgc tgcgtttgcg acacaatacg cagcagctgg gaatgcggct ggtgctggac
    23581 ggcgtgttta accacagtgg cgattcccat gcctggtttg acaggcacaa tcgtggcacg
    23641 gggggagctt gttacaaccc tgaatcgccc tggcgcgact ggtactcgtt tagtgatgat
    23701 ggcacggcgc tcgactggct tggctatgcc agcttgccga agctggatta tcagtcggaa
    23761 agtctggtga atgaaattta tcgcggggaa gacagtattg tccgccattg gctgacagcg
    23821 ccgtggagta tggacggctg gcggctggat gtggtgcata tgctggggga ggcgggtggg
    23881 gcgcgcaata atatgcagca cgttgctggg atcaccgaag cggcgaaaga aacccagccg
    23941 gaagcgtata ttgtcggcga acattttggc gatgcacggc aatggttaca ggccgatgtg
    24001 gaagatgccg ccatgaacta tcgtggcttc acattcccgt tgtggggatt tcttgccaat
    24061 accgatatct cttacgatcc gcagcaaatt gatgcccaaa cctgtatggc ctggatggat
    24121 aattaccgcg cagggctttc tcatcaacaa caattacgta tgtttaatca gctcgacagc
    24181 cacgatactg cgcgatttaa aacgctgctc ggtcgggata ttgcgcgcct gccgctggcg
    24241 gtggtctggc tgttcacctg gcctggtgta ccgtgcattt attacggtga tgaagtggga
    24301 ctggatggta aaaacgatcc gttttgccgt aaaccgttcc cctggcaggt ggaaaagcag
    24361 gatacggcgt tattcgcgct gtaccggcga atgattgtgc tgcgtaagaa aagtcaggcg
    24421 ctgcgtcgtg gcggctgtca ggtgctgtat gcggaagata acgtagtggt atttgtccgc
    24481 gtgctgaatc agcaacgtgt actggtggca atcaaccgtg gcgaggcctg tgaagtggtg
    24541 ctacccgcgt caccgtttct caatgccgtg caatggcaat gcaaagaagg gcatgggcaa
    24601 ctgactgacg ggattctggc tttgcctgcc atttcggcta cggtatggat gaactaacgt
    24661 tttataacgc cttgtgtgac gcctgcgcca tcatccgcgg ataaaactgc cagaagcggg
    24721 ttccgagggc ggtataatgg gcgtttaaat cgtaccagga gtcacgcagg gcatccagac
    24781 gtgggcgacg gcttgccatg ccgtttaaca cgttctggat gaaatccata tcgcgatagc
    24841 gcaccagcca ctgctctgac cacaagtaat tgtttagatt gataaaacgt ggcggtgagt
    24901 ccggcaaaat cgtcatcact tgcttgcggg cataacagat aaattcctgt agcggaaagt
    24961 ccggcgacag ctgcgaccag tggcgggaaa gaaagtgatc ccacatgaca tccagcgtaa
    25021 taggcgcaac gcggcgcgtt tcacgacgaa accactcccg tgcttcgcgg acttccggca
    25081 gattgtcagt caatacgtcg atacgtcgat gcatatgaat gccagccacg acgtcgggcg
    25141 gaaaactttc ttcaggattt ccgcgtacga aatcagccag taaattgccg gaaagcgagc
    25201 tttccgcgag atgggctaaa tgcaggtgag ctaaaaaatt catcgattat attctatcca
    25261 aaggggggta aaggttgcag ggagagcgcc ccggcactag actacccgcc tcttatttta
    25321 gtctgagtca gtgtcatgcg cgttaccgat ttcttctttg aattgcccga atccctgatt
    25381 gcccactacc ccatgcctga acgcagtagc tgtcgtttac tgtcgctgga cgggcctacg
    25441 ggcgcgctga cgcacggtac tttcaccgat ttactcgata agctcaaccc cggcgatctt
    25501 ctggttttta ataatactag cgtgatcccg gcgcgcctgt ttgggcgtaa agccagcggc
    25561 ggcaagattg aagtgctggt tgagcggatg ctcgacgaca aacgcattct tgcgcatatt
    25621 cgcgcctcga aagcgccaaa acctggcgca gaactgctgc tgggcgatga cgaaagtatt
    25681 aacgcaacaa tgaccgcgcg ccacggcgca ctgtttgaag tcgaattcaa tgatgatcgt
    25741 tcggtactgg atatcctcaa cagtatcggg catatgccgc tgccgccgta tatcgaccgt
    25801 ccggacgaag acgctgaccg cgaactttat caaaccgttt atagcgaaaa accgggcgcg
    25861 gttgcagccc cgaccgctgg tctgcatttt gacgagcctt tgctggaaaa attgcgcgcc
    25921 aaaggcgtgg agatggcgtt tgtgacgttg cacgttggtg cgggcacctt ccagccggtg
    25981 cgcgtcgaca ccattgaaga tcacatcatg cactcggaat acgctgaagt accgcaggat
    26041 gtggtagacg cggtactggc ggcgaaagcg cgcggtaacc gggtgattgc ggttggcacc
    26101 acttcagtac gttcgctgga aagcgcggct caggcagcga aaaacgatct cattgaaccg
    26161 ttcttcgacg atacccaaat ctttatctat ccgggcttcc agtacaaagt ggtcgatgcg
    26221 ctggtgacga acttccactt gccagagtcg acgctgatta tgctggtttc ggcctttgcc
    26281 ggttatcaac acaccatgaa cgcctataaa gcagcggtag aagagaaata tcgctttttt
    26341 agttacggtg atgcgatgtt tattacgtac aatccgcagg caattaatga gcgcgtcggg
    26401 gagtaattcc gcggcgctgg tttaaaacgt tggactgttt ttctgacgta gtggagaaaa
    26461 aatgaaattt gaactggaca ccaccgacgg tcgcgcacgc cgtggccgcc tggtctttga
    26521 tcgtggcgta gtggaaacgc cttgttttat gcctgttggc acctacggca ccgtaaaagg
    26581 gatgacgccg gaagaagttg aagccactgg cgcgcaaatt atcctcggca acaccttcca
    26641 cctgtggctg cgcccgggcc aggaaatcat gaaactgcac ggcgatctgc acgattttat
    26701 gcagtggaag gggccgatcc tcaccgactc cgggggcttc caggtcttca gccttggcga
    26761 tattcgtaaa atcaccgaac agggcgtgca cttccgtaac ccgatcaacg gcgatccgat
    26821 tttcctcgat cctgaaaaat caatggagat tcagtacgat cttggttcgg atatcgtcat
    26881 gatctttgat gagtgtacgc cgtatcctgc tgactgggat tacgcaaaac gctccatgga
    26941 gatgtctctg cgttgggcga agcgtagccg tgagcgtttt gacagtctcg gaaacaaaaa
    27001 tgcgctgttt ggtatcattc agggcagcgt ttacgaagat ttacgtgata tttctgttaa
    27061 aggtctggta gatatcggtt ttgatggcta cgctgtcggc ggtctggctg tgggtgagcc
    27121 gaaagcagat atgcaccgca ttctggagca tgtatgcccg caaattccgg cagacaaacc
    27181 gcgttacctg atgggcgttg gtaaaccaga agacctggtt gaaggcgtac gtcgtggtat
    27241 cgatatgttt gactgcgtaa tgccaacccg caacgcccga aatggtcatt tgttcgtgac
    27301 cgatggcgtg gtgaaaatcc gcaatgcgaa gtataagagc gatactggcc cactcgatcc
    27361 tgagtgtgat tgctacacct gtcgcaatta ttcacgcgct tacttgcatc atcttgaccg
    27421 ttgcaacgaa atattaggcg cgcgactcaa caccattcat aaccttcgtt actaccagcg
    27481 tttgatggcg ggtttacgca aggctattga agagggtaaa ttagagagct tcgtaactga
    27541 tttttaccag cgtcaggggc gagaagtacc acctttgaac gttgattaat attaataatg
    27601 agggaaattt aatgagcttt tttatttctg atgcggtagc ggcaacgggt gcaccggcgc
    27661 aaggtagccc gatgtctttg attttgatgc tggtggtatt cggtctgatt ttctatttca
    27721 tgatcctgcg tccacagcag aagcgcacca aagaacacaa aaagctgatg gactccattg
    27781 ccaaaggtga tgaagttctg acgaacggtg gcctggttgg tcgcgtaacc aaagtagcgg
    27841 aaaacggcta cattgctatc gcgctgaatg acaccactga agtagttatt aaacgtgact
    27901 tcgtagctgc cgtcctgccg aaaggcacca tgaaggcgct gtaattaaaa tttttcccta
    27961 agggaattgc cgtgttaaac cgttatcctt tgtggaagta cgtcatgctg atcgtggtga
    28021 ttgtcatcgg tctgctgtat gcgcttccca acctgtttgg tgaggatccg gctgttcaga
    28081 tcactggtgc gcgcggagtc gccgccagtg agcaaacgct gatccaggtc cagaaaacgt
    28141 tacaagaaga aaaaataact gctaagtctg tggcactgga agagggcgct attcttgcgc
    28201 gcttcgactc cactgacacc cagctgcgcg ctcgtgaagc attaatgggc gttatgggtg
    28261 acaaatacgt cgtggcgctt aaccttgccc cggcaacgcc gcgctggctg gcagctattc
    28321 acgctgagcc gatgaagctc ggccttgacc tgcgtggcgg cgttcacttc ctgatggaag
    28381 tggatatgga caccgcgctt ggcaaactcc aggaacaaaa tatcgatagc ctgcgcagcg
    28441 atctgcgtga aaagggcatc ccgtatacca ccgttcgtaa agaaaacaac tacggcctga
    28501 gcattacttt ccgcgatgct aaagctcgtg atgaagccat tgcgtatctg agcaagcgcc
    28561 atccggacct ggtgattagt agccagggca gcaaccagct gcgtgcggta atgagcgatg
    28621 ctcgtctgag tgaagcgcgt gaatatgcgg tgcagcagaa catcaatatc ctgcgtaacc
    28681 gtgtaaacca acttggcgta gcggagccgg tggttcagcg tcagggtgct gaccgtatcg
    28741 ttgttgaact gccaggtatt caggacactg cgcgtgcgaa agagattctg ggtgcgacgg
    28801 caacgctgga attccgtctg gtaaacacca acgttgacca ggccgctgcg gcatccggtc
    28861 gcgtaccggg cgactctgaa gtgaaacaga cccgcgaagg tcagccagtt gtgctgtaca
    28921 aacgcgtaat tctgaccggt gaccatatca ccgactccac ttccagccag gacgaataca
    28981 accaaccgca ggttaacatc tcgctcgata gcgctggtgg taacatcatg tctaacttca
    29041 ctaaggacaa catcggcaaa ccgatggcaa ccctgtttgt ggagtacaaa gacagcggta
    29101 agaaagatgc aaatggtcgt gcggttctgg tgaaacagga agaggtgatt aacatcgcca
    29161 acatccagtc tcgtctgggt aacagcttcc gtatcaccgg catcaacaac ccgaacgaag
    29221 cccgtcagct gtcactgctg ctgcgtgccg gtgcgttgat cgcgccgatt cagattgttg
    29281 aagaacgtac cattggccca accctgggta tgcagaacat tgaacagggg ctggaggctt
    29341 gccttgccgg tctgctggtt tctattctgt tcatgatcat cttctataag aagtttggtc
    29401 tgattgcgac cagtgctctg attgccaact tgatcttaat cgtcggcatt atgtcgctgt
    29461 tgccaggcgc aacgctgagt atgccaggta tcgcgggtat cgtcttaacc cttgcggtgg
    29521 cggtcgatgc gaacgtactg atcaacgaac gtattaaaga agagttgagc aacggacgta
    29581 ctgttcaaca ggcaattgat gaaggttatc gtggcgcatt tagttctatc ttcgatgcga
    29641 acatcaccac gctgattaaa gtcatcatcc tgtacgcagt gggtaccggg gcaattaaag
    29701 ggttcgcgat tactaccggt atcggtgtgg cgacgtcgat gtttaccgcg attgtcggta
    29761 cgcgtgccat cgtaaacctg ctatatggcg gcaagcgcgt caagaagctg tcaatctgag
    29821 gagtgcgatg tggcacagga atatactgtt gaacaactaa accacggccg taaagtctat
    29881 gactttatgc gctgggacta ctgggctttc ggcatctctg gtctgctgtt aatcgccgct
    29941 atcgttatta tgggcgtgcg cggctttaac tgggggctgg atttcaccgg tggtacggtt
    30001 atcgaaatta cgctcgaaaa accggctgaa attgacgtaa tgcgtgatgc attgcaaaaa
    30061 gccggttttg aagagccaat gctgcaaaac tttggtagca gccacgacat catggtccgt
    30121 atgccgcctg ctgaaggcga aaccggcggt caggtgctgg gcagccaggt tctgaaggtg
    30181 attaacgaat ccaccaatca gaatgcagca gtgaagcgta ttgagttcgt cggtccgagc
    30241 gtgggggaag accttacgca aaccggtgcg atggcgttga tggcggcgct gctgtctatc
    30301 cttgtgtacg taggttttcg ctttgagtgg cgactggcgg caggggttgt tattgcgctg
    30361 gcgcacgacg ttatcattac gctgggtatt ttgtcgttat tccatatcga gattgacctg
    30421 accattgtgg catcgttgat gtcggttatc ggttactcgc ttaacgacag tatcgtggta
    30481 tcggaccgta ttcgtgaaaa cttccgcaag atccgtcgcg gtacgcctta cgaaatcttt
    30541 aacgtgtcct tgacccagac gctgcaccgt accttgatca catccggtac taccttgatg
    30601 gttatcctga tgctgtacct cttcggtggt ccggtactgg aaggcttctc gctgaccatg
    30661 cttatcggtg tttccatcgg tactgcatct tccatctatg tggcatctgc gttggctctg
    30721 aaactgggta tgaagcgcga acacatgttg cagcagaaag tggaaaaaga aggggcggat
    30781 cagccgtcaa ttctgccgta atcaagttcc cgttgatgtt gaaaatcccg gtcagaagat
    30841 cgggattttt tttgatgtat ggagattgcg aaaatccccg catcttggga aactgcgcgt
    30901 aaccctacat ttcatccagg taactcttca tggctatcat cccaaaaaac tatgcgcggt
    30961 tagaaagcgg ctatcgcgaa aaagcattaa aaatatatcc gtgggtctgc ggtcgctgtt
    31021 cccgcgagtt tgtttattcc aacctgcgtg aacttaccgt tcaccacatt gatcacgacc
    31081 ataccaataa cccggaagat ggcagtaact gggaattgct ttgtctctat tgccacgatc
    31141 atgagcattc gaaatatacc gaagcggatc agtatggtac gaccgttatc gcaggggaag
    31201 atgcgcagaa agatgtcggt gaagcgaagt acaacccatt cgctgacctg aaagcgatga
    31261 tgaacaagaa gaagtgatta aaacgtaaaa ttgcctgatg cgctacgctt atcaggccta
    31321 cgttatttca gcaatatatt gaatttacgt gcttttgtgg gccggacaaa gcgtttacgc
    31381 cgcatccggc atgaacaaag ctcacgttgt taacaatcag aaatgccggg aataaatccc
    31441 ggcattttca taatcagaag ttgtaaccta ctaccaggta accaccccag ccggtagagc
    31501 gaacgttgaa gttgccgttg ccgaagttca gttctgcatc gtcgttccac tgaccaccgt
    31561 cgtgccagta acgagctacg acagagtagt gccagtgatc gtagttcaga gccagaatat
    31621 ggctggaagc gatagagtta ttagtacggg tcttaatacc gttgattgcg ttaccgctgt
    31681 catcccctaa atcggaaccc cagtcgaagt tggtgaagcc gatgtagctc agctgaccgc
    31741 cccacagatc ggtaatcggc acaaagtatt taattttgaa acggtaaccg tcccactcgt
    31801 tttcgttcgc tgcgccatag ttctgccact ggtatttcgc atagacgttc atggacaggc
    31861 tcatcggcag atcagtatcg atgtcggtac ccagacccat gtaccaggtg ctctggcgac
    31921 catttttatt acgacccatg tcgtaaatgt agttgttcgc gaagtaccac tctttgaacg
    31981 gaccgaagtt aaggtcagta ttggtcagct tgtcgatgga gaaacgtggt tcgatttcca
    32041 taaacagcgg agaaccgtgg ttccagatac ctttagcatc ggagttaccg ccgaagaata
    32101 ccggcgcatc cgcataacca tagaagtcga accagtcttt tttagcgaat gcttcgtact
    32161 caaggtaggt atcgttgcgg atctgcggtc cgaaacgggt gtgatagctt ccgacaacgt
    32221 taacgctctg gtgccaccag tcggaaagat actgcggttt gtcgttttca gctgcgttga
    32281 cagtaaaaga cgaagagagc gccagtaccg caccggctgc cagtaatgtt tttttcatat
    32341 gtatgccact gtttgaaaat cccttgcggg agtgaaaaag gcgcaaattg cgtttctaaa
    32401 tatttcgtat acagaggagc ctattataaa aatcattgct cacaaaaata tgtttcgttt
    32461 cacagttcta tcatttacgt aatcgattgc gttcacgttt acacacattc gggcggggat
    32521 tgtactgact ttcgcccttg ttgcaaactt ttactatcaa tccaggtgat gtaagcagag
    32581 taacaaaatg acggggaaag atgttgcggg acgcacaaaa tcgtcccgaa atacgtttac
    32641 tgcataacag gttgaatgtc gtggatacgt acaaagccta actgatccac agaaatcccc
    32701 tttaattgta acggaatatc aacatcgctg ggggcgagga cgctggcagg ggcattaatc
    32761 aattgattct gcacattgat ttcctgaaag ttgtctattg ttccctgaat ttgcccatat
    32821 tcaacagttc cgctgaatgc gggcaaggga tcattcgact caccctgaat acgtagtgta
    32881 agggtggtgc catctgtatc aggcgtaata ttcaccagcg acatacgtaa agtaccgatc
    32941 tggctttcca gtcttgccgg tgttttcgct ctaggcagaa ggtaaccccc actgctggat
    33001 tttgcattca gcctgttttg ctgagtgatt ttgactgttt cttgattgag ttgcgtcatc
    33061 tcctggttca gcgtgctgac gctgtgtttc atctgtcgca cttcactttg ctgcacacag
    33121 gcgctaaggc tgaacaggct tcccagcagg agcagtcgaa aaacgttagt attcataggt
    33181 cgtccctttc tcgtcgcaat tagcctaatg gtagagggta aaagcagcga aagcatcgat
    33241 cctttatctc aaaagcgttg cgcctttgtt gtatcgtcag ttcagggtaa aatagatttc
    33301 cgttaaccac ttggtcagga cgccgtatgc attgcccatt ctgtttcgcc gtggacacta
    33361 aggtaattga ctctcgtctc gtgggcgagg gttcatccgt acgccgccgt cggcagtgtc
    33421 tggtgtgtaa tgaacgtttc accacctttg aagtggcgga gctggttatg ccgcgtgttg
    33481 taaaaagcaa cgacgtgcgt gaaccgttta atgaagagaa attgcgtagc ggaatgctgc
    33541 gggcgctgga aaaacgtccg gtgagttccg atgacgttga aatggcaatc aatcatatta
    33601 aatcgcagct gcgcgccacc ggtgagcgcg aagtaccgag caagatgatt ggcaatctgg
    33661 tgatggagca attgaaaaag ctcgataaag tcgcctatat ccgttttgcc tctgtctacc
    33721 gcagtttcga agatatcaaa gaatttggcg aagagatcgc gcgcctggag gactaagccg
    33781 tgcaggacga gtattacatg gcgcgggcgc taaagctggc gcaacgagga cgttttacca
    33841 cccaccccaa cccaaatgtc gggtgcgtca ttgtcaaaga tggcgaaatt gtcggtgaag
    33901 gttaccatca acgagcgggt gaaccacatg ccgaagtaca cgcgttgcgt atggcgggtg
    33961 aaaaagccaa aggtgcaacg gcgtatgtga cactcgaacc ttgcagccat cacggccgta
    34021 cgccgccttg ctgtgatgct ttaattgccg caggtgtggc gcgcgtggtt gcctcgatgc
    34081 aagatcctaa cccgcaggtc gctgggcgtg gactttaccg tctgcaacag gctggcattg
    34141 acgtcagcca cggcctgatg atgagtgaag ccgagcaatt gaataaaggc tttctcaagc
    34201 ggatgcgcac cggctttcct tatattcagt taaaacttgg cgcatcgctt gatggtcgca
    34261 cggcgatggc gagcggcgaa agccagtgga tcacttcgcc ccaggcgcgg cgtgatgtac
    34321 aacgactgcg cgcgcaaagt catgccattt taaccagcag cgccacggtg ctggcggatg
    34381 atcctgcctt aacggtgcgt tggtccgaac tggatgaaca aactcaggcg ctctacccgc
    34441 aacaaaatct ccgccagccg gtacgtattg tgattgatag ccaaaatcgc gtgacgccgg
    34501 aacatcacat tgtgcagcag cccggcgaaa cctggttcgc gcgtacgcag gaagattctc
    34561 gtgagtggcc ggaaacggtg cgtaccttgc tgattccaga gcataaaggt catctggatc
    34621 tggttgtact gatgatgcaa ctgggtaaac agcagattaa cagcatctgg ggggaagcgg
    34681 ggccaacgct cgctggcgca ttgctgcagg cgggtttagt cgatgagctg attgtctata
    34741 tcgcacctaa actattaggc agcgacgccc gtggattatg caccctgcca gggcttgaga
    34801 aattagccga cgccccccaa tttaaattca aagagatacg tcatgtaggc ccggatgttt
    34861 gcctgcattt agtgggtgca tgatctcccg gctcgaaagg gaagcagcgc acgaaatatt
    34921 atgctaaaat ccgcccccct gcggggccat actcgaaccc gaaggaagaa aatgaacatt
    34981 attgaagcta acgttgctac cccggacgct cgcgtcgcca tcaccatcgc gcgtttcaac
    35041 aactttatca atgacagcct gctggaaggt gcaattgacg cactgaaacg tatcggtcag
    35101 gtaaaagatg aaaacattac cgttgtttgg gtgcctggtg cctatgagct gccgctggcg
    35161 gcgggtgcac tggctaaaac cggtaaatac gacgcggtga ttgcgctggg tacggttatt
    35221 cgtggtggca ctgcccattt tgaatatgtc gctggtggtg caagcaacgg cctggcgcat
    35281 gttgcccagg acagcgaaat tccggttgct tttggggttc tgaccactga aagcattgaa
    35341 caagcgatcg aacgtgctgg caccaaagct ggcaacaaag gtgcagaagc tgcactgacc
    35401 gcgcttgaaa tgattaatgt attgaaagcc atcaaggcct gaaattagta aggggaaatc
    35461 cgtgaaacct gctgctcgtc gccgcgctcg tgagtgtgcc gtccaggcgc tctactcctg
    35521 gcagttgtcc cagaacgaca tcgctgatgt tgaataccag ttcctggctg aacaggatgt
    35581 aaaagacgtt gacgtcctgt acttccgtga gctgctggcc ggggtggcga ctaataccgc
    35641 atacctcgac ggactgatga agccatacct gtcccgtctg ctggaagaac tgggacaggt
    35701 agaaaaagca gtactgcgca ttgcgctgta cgaactgtct aaacgtagcg atgtgccata
    35761 caaagtggcc attaacgaag cgatcgaact ggcgaaatcg ttcggcgcag aagacagcca
    35821 taagttcgtc aacggtgtac tcgataaagc agcacctgtg attcgcccta acaaaaagta
    35881 atatccaggc cggtagattc acggaagacc gttccatgat cgccggcctt ttctttttta
    35941 cctgctgagg cataacgtat ggcatgtggc gagttctccc tgattgcccg ttattttgac
    36001 cgtgtaagaa gttctcgtct tgatgtcgaa ctgggcatcg gcgacgattg cgcacttctc
    36061 aatatccccg agaaacagac cctggcgatc agcactgata cgctggtggc gggcaaccac
    36121 ttcctccctg atatcgatcc tgctgatctg gcgtataaag cactggcggt gaacctaagc
    36181 gatctggcag cgatgggggc cgatccggcc tggctgacgc tggcattaac cttgccggac
    36241 gtagacgaag cgtggcttga gtccttcagc gacagtttgt ttgatcttct caattattac
    36301 gatatgcaac tcattggcgg cgataccacg cgtgggccat tatcaatgac gttgggtatc
    36361 cacggctttg ttccgatggg acgagcctta acgcgctctg gggcgaaacc gggtgactgg
    36421 atctatgtga ccggtacacc gggcgatagc gccgccgggc tggcgatttt gcaaaaccgt
    36481 ttgcaggttg ccgatgctaa agatgcggac tacttgatca aacgtcatct ccgtccatcg
    36541 ccgcgtattt tacaggggca ggcactgcgc gatctggcaa attcagctat cgatctctct
    36601 gacggtctga tttccgatct cgggcatatc gtgaaagcca gcgactgcgg cgtacgtatt
    36661 gacctggcat tgctgccgtt ttctgatgcg ctttctcgcc atgttgaacc ggaacaggcg
    36721 ctgcgctggg cgctctctgg cggtgaagat tacgagttgt gtttcacggt gccggaactg
    36781 aaccgtggag cgctggatgt ggctctcgga cacctgggcg taccgtttac ctgtatcggg
    36841 caaatgaccg ccgatatcga agggctttgt tttattcgtg acggtgaacc tgttacgtta
    36901 gactggaaag gatatgacca ttttgccacg ccataaagat gtcgcgaaaa gtcgcctgaa
    36961 gatgagtaat ccgtggcatc tacttgctgt cggattcgga agtggattaa gcccgatcgt
    37021 tcctgggacg atgggctcgc tggcagcgat tccgttctgg tatctgatga cctttttgcc
    37081 ctggcagctc tactcgctgg tggtgatgct ggggatctgt atcggcgtct atctttgtca
    37141 tcagaccgca aaagatatgg gcgtgcacga tcacggcagc attgtctggg acgaatttat
    37201 tggtatgtgg atcacgctca tggcgctgcc gaccaatgac tggcagtggg ttgccgccgg
    37261 gtttgtgatt ttccgtattc tggatatgtg gaaaccgtgg ccgatccgct ggtttgatcg
    37321 caatgtgcat ggcggcatgg ggatcatgat agatgatatt gtcgccgggg tgatctccgc
    37381 tggcatcctg tactttattg gtcatcactg gccgctgggc attctgtcgt agttgtgcac
    37441 cgatgcctga tgtgacgctt gttgcgtctc atcaggcctg gactcttatt taaatcctac
    37501 taccggatgc ggtttatacg gcgtttccag ttcggcaatc tgttccggct tcaaagtgat
    37561 atccaccgcg ttcaatagct catcaagctg ttcttcccgc gatgtaccga taatcggtgc
    37621 ggcaatgccc ggtttactca acaaccaagc cagcgcaact tgtgctcgtg ttgcaccgag
    37681 ttcttcactg acgcccgtta accgctctgc gatctgcgcg tcattttcat cgctttcttt
    37741 atagagattt ttccccacct catcagacac cagtcgtgcg gtagtttctc cccacggacg
    37801 cgtcagtcgc ccccgcgcca gcgggctcca cggaatcacc gccacgccct cctgataaca
    37861 cagtggcagc atctcgcgct cttcttcgcg ataaatcaga ttgtagtgat cctgcatact
    37921 gacaaactgc gcccagccgt gctgtttttg gagttccagt gcctgagcaa actgcgaagc
    37981 gtgcattgat gacgcgccga tataacgcgc tttcccggct tttaccacgt cgttcagggc
    38041 ttccagcgtc tcttcgatcg gcgtgttgta atcccagcga tgaatttgca ggatatcgac
    38101 ataatccatg ccgagacgtc gcaggctgtc gtcgatagag cgcaaaattt gtgcacggga
    38161 taatccttcc ggtaaatcac caacgcgatg gaacactttg gtggcaacga ccacgtcttc
    38221 acgacgggcg aaatcccgca gtgcgcgacc aacgatctct tcgctgctac catcggaata
    38281 gctattggcg gtatcaaaga aatttatgcc gccttccagt gcgcgtttaa ttatgggacg
    38341 gctgctctct tccggcagtg tccatgcgtg attaccacga tctggctcgc caaaggtcat
    38401 acagccgagg caaagtcggg aaacgcgaag gtcggttttt cctaaggggt tgtattgcat
    38461 gctgccactc ctgccatgaa taatttctta agcatagcag gagtggagta gggattatgc
    38521 cagccaggcc ttgattttgg cttccatacc ggcggcatcg aggccgagtt cggcgcgcat
    38581 ttcttcctgc gttccttgcg gaataaagaa gtcaggcagg ccaatgttca gcacggggac
    38641 tggtttacga tgggccatta gcacttcgtt cactccgctg cctgcaccgc ccataatggc
    38701 gttttcttct acggtaacca gtgcttcatg actggcggcc atttccagaa ttaacgtttc
    38761 atcaagcggt ttcacaaaac gcatatcgac cagcgtggcg ttcagcgatt cggcgacttt
    38821 cgccgcttct ggcatcagcg taccaaagtt aaggatcgcc agtttctcgc cacgacgctt
    38881 cacaatgcct ttgccaattg gtagtttttc cagcggcgtc agttccacgc cgaccgcgtt
    38941 gccacgcggg tagcgcaccg ctgacgggcc atcgttatag tgatagccgg tatagagcat
    39001 ctggcgacat tcgttttcat cgctcggggt cataatgacc atttccggta tgcagcgcag
    39061 gtaagagaga tcaaaagcac cctgatgggt ttgaccgtca gcaccaacaa tgcccgcgcg
    39121 gtcgatggcg aacaggaccg gaagcttttg aattgccacg tcatgcagca cctgatcata
    39181 ggcgcgttgc aggaaagtgg agtagatcgc gacaatgggt ttgtacccac caatcgccag
    39241 accggcggca aaggtcactg cgtgttgctc ggcaattgcc acgtcgaagt agcgatccgg
    39301 gaatttacga gaaaactcga ccatgccgga accttcacgc atcgccggag taatcgccat
    39361 cagcttgtta tctttcgccg ccgtttcgca caaccagtcg ccaaagattt ttgaatagct
    39421 cggtaaaccg ccgctacttt tcggcaaaca accgctggag ggatcaaatt taggcacggc
    39481 gtggaaagtg attgggtctt tttctgccgg ttcataacca cgaccttttt tggtcatgat
    39541 atgcaggaac tgcgggcctt tcaggtcacg catgttcttc agcgtggtga taagccccag
    39601 cacatcgtga ccgtcaaccg ggccgatgta gttaaagccc agctcttcaa acaacgtgcc
    39661 agggactacc atgcctttaa tatgttcttc ggtacgtttg agcagctctt taatgggtgg
    39721 cacgccagag aaaacttttt tcccgccttc gcgcagtgaa gagtaaagct taccggaaag
    39781 cagctgcgcc agatggttgt tgagcgcgcc gacattttcg gaaatcgaca tttcgttgtc
    39841 gttgaggacc accagcatat caggacggat atcgcccgcg tgattcatcg cttcaaacgc
    39901 catgcctgcg gtaatcgcgc catcgccgat tacacagacg gtacggcgat ttttgccttc
    39961 tttctcggca gcaaccgcaa taccaattcc ggcactgatg gaggttgatg aatgcccgac
    40021 gcttaatacg tcatattcgc tttcgccgcg ccacgggaac gggtgcaggc cgcctttctg
    40081 acggatggtg ccgattttgt cgcggcgtcc ggtcaaaatt ttatgcggat aagcctgatg
    40141 ccccacatcc caaatcaatt ggtcaaacgg ggtgttgtag acatagtgca gcgccacggt
    40201 cagttcgacc gtgcccagcc cggaggcgaa gtgcccgctg gaacggctca cgctgtcgag
    40261 taaatagcgg cgcagttcgt cgcagagttt cggtaaactc tctttcggca acagtcgtaa
    40321 ctcctgggtg gagtcgacca gtgccagggt cgggtatttg gcaatatcaa aactcatcag
    40381 gggcctatta atacttattg tttatttatt acgctggatg atgtagtccg ctagcgcttc
    40441 cagtgccgag gtatcgggtg actgttcagc cagttgtttc agcgactgac gggcatcgtc
    40501 gatcagatcc cgggctttct tccgggcttg ctcaagaccc agaagtgcag ggtaggtact
    40561 tttaccaagt tgctggtcgg caccctggcg ttttcccaac gttgcagtat ctcctaccac
    40621 atccaggatg tcatcctgaa cctggaaggc aaggccgatg ctctctgcgt acttgtcgag
    40681 tactggcaga gcacgacgcc ctttatctcc ggcgcttaat gcaccaaggc gaacggcggc
    40741 gcgaatcaat gcgccggttt tatgacgatg aatacgctca agcgcgtcca gaggtacgtg
    40801 tttgccttcc gcgtctaaat ctaatgcctg accaccgcac attccggcaa taccgctggc
    40861 gctcgccagt tcagaaatca tcgaaattct gtcgcgatcc gacacttccg gcatatcagc
    40921 atcgcttaaa atcgagaacg ccagcgtttg taaagcgtcg ccagcgagaa tcgcgtttgc
    40981 ttcgccaaac ttcacatggc aggtcggcaa accgcgacgc agatcgtcat catccatcgc
    41041 cggtaaatca tcatgaatta atgagtaagc gtggatacac tctacggcgg cagcgggtgc
    41101 gtccagcgtg tttgtgctaa cgccaaacat atgaccggtg gcataaacca ggaagggtcg
    41161 cagacgctta ccacctaata atgcaccata ctgcatggtt tcgaccacgg gagtgttctg
    41221 aaagggcagt ggggcgataa aacggctcag cgcctggttg gcctgcttaa cgcaggcttc
    41281 gagttgctgc ggaaagtcca ttactcattg tccggtgtaa aaggggttag agaggcgtct
    41341 tcattgtcag acagcagaat ttgtacgcgc tgttcggctt gttgtaattt ggcctgcccc
    41401 tgacgtgcca gctgcacgcc gcgttcgaac tcgttcagcg cctcttccag cggcaggtcg
    41461 ccactttcca gacgggttac aatctgttcc agctcgctca gcgccttttc aaagctggcg
    41521 ggagcctcat ttttcttcgg cataatgaat gtctgactct caatattttt tgccccgtca
    41581 tggtaacgga ctcagggcaa atagcaaata acgcgcaatg gtaaggtgat gtgcgcagca
    41641 aagcgatgtt agtggtatac ttccgcgcct ggatgcagcc gcaggtgtgg gctgctgtat
    41701 ttttccctat acaagtcgct taaggcttgc caacgaacca ttgccgccat gaagtttatc
    41761 attaaattgt tcccggaaat caccatcaaa agccaatctg tgcgcttgcg ctttataaaa
    41821 atccttaccg ggaacattcg taacgtttta aagcactatg atgagacgct cgctgtcgtc
    41881 cgccactggg ataacatcga agttcgtgca aaagatgaaa accagcgtct ggctattcgc
    41941 gacgccctga cccgtattcc gggtatccac catattctcg aagtcgaaga cgtgccgttt
    42001 accgacatgc acgatatttt tgagaaagcg ttggttcagt atcgcgatca gctggaaggc
    42061 aaaaccttct gcgtacgcgt gaagcgccgt ggcaaacatg attttagctc gattgatgtg
    42121 gaacgttacg tcggcggcgg tttaaatcag catattgagt ccgcgcgcgt gaagctgacc
    42181 aatccggaag tgactgtcca tctggaagtg gaagacgatc gtcttctgct gattaaaggc
    42241 cgctacgaag gtattggcgg tttcccgatc ggcacccagg aagatgtgct gtcgctcatt
    42301 tccggtggtt tcgactccgg tgtttccagt tatatgttga tgcgtcgcgg ctgccgcgtg
    42361 cattactgtt tctttaacct tggcggcgcg gcgcatgaaa ttggcgtgcg tcaggtggcg
    42421 cattatctgt ggaaccgttt tggcagctcc caccgcgtgc gttttgtcgc tattaatttc
    42481 gaaccggtcg tcggggaaat tctcgagaaa atcgacgacg gtcagatggg cgttatcctc
    42541 aaacgtatga tggtgcgtgc cgcgtctaaa gtggctgaac gttacggcgt acaggcgctg
    42601 gtcaccggcg aagcgctcgg ccaggtgtcc agtcagacgc tgaccaacct gcgcctgatt
    42661 gataacgtct ccgatacgct gatcctgcgt ccgctgattt cttacgacaa agagcacatc
    42721 atcaacctag ctcgccagat tggcaccgaa gactttgctc gcacgatgcc ggaatattgc
    42781 ggtgttatct ccaaaagccc gacggtgaaa gcggttaagt cgaagattga agcggaagaa
    42841 gagaaattcg acttcagcat tctcgataaa gtggttgaag aagcgaataa cgttgatatc
    42901 cgcgaaatcg ccctgcagac cgagcaggaa gtggtggaag tggaaaccgt caatggcttc
    42961 ggcccgaacg acgtgatcct cgatatccgt tctatcgatg aacaggaaga taagccactg
    43021 aaagtcgaag ggattgatgt agtttctctg ccgttctata aactgagcac caagtttggc
    43081 gatctcgacc agaacagaac ctggctactg tggtgtgagc gcggggtgat gagccgtctg
    43141 caggcgctct atctgcgcga gcagggcttt aacaatgtga aggtatatcg cccgtaattt
    43201 gtggttgtaa cgtcgcatct ggtcagatgc gacgcttacc gcatccgaca ctactcgtaa
    43261 taattataaa tccctgccgc catcaccagt tgtgatgcca cttcatgggc tttctcgcgc
    43321 ccaacctgca ggtcgataat tttcagacca aagtcgatag ctgtccccgg cccctggctg
    43381 gtcagcaatt ttacccgtgc atcccagacg acgcgcttgt cctgccattg ttcagcggga
    43441 attttgtctt tcagcgtcgg gaagccggtc atattaccaa tcgggaagat atcgtgcggc
    43501 accagcacgg tggctggcgc ggcgcaaata gccgcgacga tacgcccgga acggtggaac
    43561 tgtttaacgg tttcaaccag cagagtgcta tcgcgaaaac actctgcgcc tttaatgcca
    43621 ccaggcagta cgatcacgtc atattcgcca tcagccacct cgaccagcgg cgcatccgcc
    43681 agcagcttca cgccgcgcga gcaggtaatt gccaggttgc catcgctggc tacgctggca
    43741 gtggtgactt tgataccgcc gcgaaccaag aggtcgatcg tggtgacggc ttcagtctct
    43801 tcactaccag gggcgaggca aaccagtgcc gatgcgctca tattcactct cctttctttt
    43861 taccatttca aacaggcggg tgttttccgg tacggcaatc ccatgcgcgc gggcgcggcg
    43921 taagagaaaa ccattgatat agtcgatttc ggtgtggcgc agcgcgcgga tatcctgcaa
    43981 catcgacgag atattttccg ctgtggcatc aatcacctgc atcacgtaat cacgcaaatc
    44041 ttccgctgaa gtatgatgcc cttcgcgttc gatcactgcc gcgacttctt cgcatatttg
    44101 cataatttct tgcggatgat gacgtaattc accgttcggg caattccaga tggcggtcag
    44161 tggattaatc acgcagttga ctgccagctt gcgccacagc tcggcgcgaa tattgttatg
    44221 ccaggcaacg tcaggcaaca cggtttgtaa aatatccgcc agataactgt aatcgccatc
    44281 ctgttgccgt gccggaccaa tatgcgtgat accgtttgcc acatgaataa tgacattgcc
    44341 gtcgcggcgg gcggcatggg tggtggtgcc catcagtaat ggctgctgaa tattttgcaa
    44401 ctcttcgatg gtgcccatgc cgttgtgaat taacagtatt ggcgtggtta caggcagtgt
    44461 ggacgcgagg cttttgacgg catcggaaac ctgccatgct ttcagcgtca ccaggagcag
    44521 atcgctggtg gcgagaaaat cgggatcgtt ggcggtcagc gattcgttaa atatcgaacc
    44581 atctgtctca accagattcg cactacaata aggttgcggt acgcgcagcc agccctgaac
    44641 ttcatgaccc tgtttgcaaa gtgctgtaag ccataattgc cctaaggcac cgcatcccaa
    44701 tacggtaatt ttcattgttc ctcctcaccc gcaaccactc cgggtgttca ataaggctat
    44761 cccttaattg tgcatgctgt tgcgactatg cacaattaag ggatacgtcc tggtgcagga
    44821 ctgtcggtta tttaactttg cgggtattat gcttcgcatc aaaaatgaag ggagaggaaa
    44881 agatgccatc tttcgatatt gtctctgaag ttgatcttca ggaagcacgt aacgcggtcg
    44941 ataacgcgag ccgcgaagtg gagtcccgtt ttgacttccg taacgttgaa gcctcatttg
    45001 agctgaacga cgccagcaaa accatcaaag tgttgagcga gtccgatttc caggtcaatc
    45061 agttgctgga tattctgcgt gccaagctgc tgaagcgcgg cattgaaggc agttcactgg
    45121 atgtaccgga aaatatcgtt catagcggta aaacctggtt tgtggaagcg aaactgaagc
    45181 agggcattga gagcgcgacc cagaaaaaaa tcgtcaagat gatcaaagac agcaaactga
    45241 aagtgcaggc gcaaattcag ggcgatgaga tccgcgtgac tggcaaatct cgtgatgatt
    45301 tgcaggctgt catggcgatg gtgcgcggtg gcgatctggg tcagccgttc cagttcaaaa
    45361 acttccgcga ttaatcgcga caacctgcgc tttgttcatg tcagatgcgg catgaacgcc
    45421 tggttcggcc tacaaaggct tgtaaatcag gagcttagta ggcctgatga gcgtagcgca
    45481 gcaggcaatt tcgcctcgct tttatgcctg gcgaattgcc tgttcaacat caaagcgatt
    45541 cgtcactttg ctgtcgattt tcacataagc tgaatgttct tcttctgcaa tcaacacttc
    45601 tttgatgcct tcagtttcta gcaaacgcac ttttaacgcc tcgtttgcgg caatgttcgc
    45661 cgggatttca atgcgcaaac tgctgacata cgccggttct ttcatggtac tggcgactgc
    45721 cagccacact gcggccagca ttgcgccagc gagaaatacg ccctgaccgt caaacatgcc
    45781 gtcaatccag ccgcccagtg aaccgccaat cgccacgcca agaaactggc tggtggagta
    45841 aacacccatc gctgtacctt tgtaacctgc tggcgactct ttactgataa gtgaaggcag
    45901 aagggcttcc atcaaattaa acgctacaaa gaaaagctgc acgccgacca ccagttgcca
    45961 gaactgcgtt tgcgcgttcc acaacacaat ttccgcaacc acgatcaatc cgacgcagaa
    46021 gacaaagact tgcttcattt tgcgcttaac ttcagcgtag ataatgaaag gcacgaccga
    46081 gccaaaggcg attagcattg tcgccagata gaccttccag tgttcagccg ccgggaaccc
    46141 tgcatcagcc agttgtccgg gcagggcaac aaacgtcgac atcagcaaaa tatgcagaca
    46201 cataatgcca aagttgagtt tcagcagccg cggttccgcc agcactttac tgaaactgcc
    46261 tttcaccatt ccggactcac gattaagtac gtgagtgcta ctgttgggca caacccaaat
    46321 ggtcaacgca atgccggtcg ttgccagaat agcgatcatc cagaacagcg cgtgcagccc
    46381 aagtttgtga gtgatgatcg ggccaagcac catcgcaatg gcaaaggtaa tgccaaagct
    46441 cacgccgata aacgccattg ctttggtgcg gttttgttcg cgcgtgagat cggaaagcag
    46501 cgccataacg gcagcggcaa tcgcaccgga gccttgtagc gcccggccta gaataattcc
    46561 ccagatggag tcagagagcg cagcgataac gctaccggcg gcaaacaccg ccagcccacc
    46621 gacaattaat ggtttgcgac caatacggtc ggaaagcagg ccaaacggaa tctgaaaaac
    46681 ggcctgagtc agaccataaa taccaatggc aataccgatt aatgcttcgc tggcaccttg
    46741 cagagccatg ccatacgtgg tcagaaccgg cagaaccata aacatgccca gcatgcgcaa
    46801 cgagaatacg gtccctaaac cccaggtcgc gcgcctctca cctggcgtca ttttataatc
    46861 gttcattacc acctctgttt taaattcgcg actagtgtaa agcggcaaac gaatggggta
    46921 aacatgcgga ttttttagta aatatttcga gagggatatg tttctaatgc taagaaaaaa
    46981 ggtgccgtag caccttttta atagagaggt tttgttacca cacagcagcc agcagcgtat
    47041 gcgagtccgg taccataaaa tcaacggaca tcatcaccga gagggcagtg atggcgatga
    47101 tagagaagcc gaacagcttg cgcgcccaga ttctgtcatc agcaacttta taaccgcgca
    47161 gagccatacc taaccaccag acgctaaccg ccgcggcgac caccagatat ttatatccag
    47221 cgtaaccgcc aagagagagc atcagcgtgg caacggcaaa ggcgatgata tacagcgtga
    47281 tgtgattctt cgccaccgaa atgcctttta ccactggcaa taccggaatg tttgccgcct
    47341 ggtaatcctt aaagcggaaa atggcgatgg cataggagtg aggcatctgc cacaggctga
    47401 agatagccag caggatcgct gcgccgctat cgaactcgcc ggttactgca cagtagccaa
    47461 tcaccggcgg cgcagcgccg gagagcgaac caatcaatgt gccgtagact gagtggcgtt
    47521 tcatgtacag gctatagacg ccgacataaa ccacaaagcc catcaccccc agccagcagg
    47581 ccagcggatt cgcgccaaac cacagcagca taaagccagc aatacccagc aaggtggcgt
    47641 acaccagcga gacagcagga gagatcaggc ctttaaccag cacccgattc ttcgtccttt
    47701 ccatctttct gtcgatatcc ctgtcgatgt agttgttaaa cacacaaccc gacgccacaa
    47761 ccagtgacac cccaaccagc gtgtagataa acaggggata atcaatgctg ccctttgagg
    47821 ccagcaggaa tcccccaatc accgagatca ggttgccaaa gatgatgcct ggtttcgtta
    47881 cttgcaggta ttgcttaaac atcataaccg ccgctcttag tgcatcatca tgttgtagtt
    47941 gaggttccac ataatccaga tggagcctac aaccaggata gtgatgatta gcacggtgaa
    48001 gacaaacgcc gtcatgttcc agccttcatc tgatttggta ttcatgtgca ggaagcacac
    48061 cagatgcacc agaacctgta ccactgccat tgccaggatt gttcccagaa ttacggccgg
    48121 agaggcagcc cctgtcatca ccatccagaa cggaatcacc gtcagaatga tcgacaggat
    48181 aaagcctgtc atgtaggttt ttacgctgcc atgggacgcg ccgctgtgat cggtagaatg
    48241 actcattaca tcgcccccat cagataaaca acagtgaaca cacagatcca aaccacatcc
    48301 aggaagtgcc agaacaggct caggcacatg atgcgggtac ggttagtgct ggtcaggccg
    48361 cgacgagcga tttgtaccat cagcaccgcc atccagataa gaccggaagt gacgtgcaga
    48421 ccgtgcgtgc cgaccagcgc aaagaacgct gacaggaagc cgctgcgatc cggacccatg
    48481 ccgttaacaa tcaggtgatg gaattcgtag atttccatcc cgataaatcc ggcaccaaac
    48541 aaacaggtca acgccagcca ggagataacc tggcttttgt tgtttttgta catggcgata
    48601 gccgccatgc cgtaggtgat ggagctgaac aacagcaaga aagtttcaac cagaacgaac
    48661 ggcagttcga aaatgtcctt acctgtcggg ccgcctgcgg tgccgttcac cagaacggca
    48721 taggtagcaa acaagattga gaacagaatg cagtcgctca tcaggtagat ccaaaatccg
    48781 aagattttgg ttccgcctgc atcgtggtgc ccgtgttcgt gcgcgtgggc agtcgcgtgc
    48841 gtcaaagtat cagttgccat ttttcagccc tgccttagta atctcatcga aatgctggtt
    48901 ttccagtttt tcgatttctg ccaccggcac gtagtaatcc acgtcctcgt cgaagctttt
    48961 cacgatccag gtgatgatca tgcctgcgaa gccaacaatc gccagccacc agatatgcca
    49021 gatcatggcg aaaccgaaga tggtggagaa agctgcaatg acgatacctg caccgctgtt
    49081 tttcggcata tgaatttctt catagtggtc aggcttttta tacgcttcgc ctttctcttt
    49141 catttcccag aatgcatcac gctcgtgaac gtgcggcaca acggcaaagt tatagaacgg
    49201 aggcggggaa gagtttgccc actccagcgt acggccaccc cacgggtcgc cagtcaggtc
    49261 acggttctgg tcgcggtcgc gaatagaaac gtacatctga ataacgaggc agagaatacc
    49321 cagcgcaatc agtactgcac cgctagctgc aatcatcagc atggtgtgga actgcgggtc
    49381 aatctgctgg ctcaaacgac gggtcatgcc catgaagccc agcgcataca gcggcataaa
    49441 ggcaacgaag aagccgatga tccagaacca gaacgcgcgt ttaccccagg tttcattcag
    49501 tttgaaaccg aacgctttag gccaccagta ggtcatccct gcgaagcagc cgaagaccac
    49561 gccgccgatg ataacgttat ggaagtgcgc aatcaggaac aggctgttat gcagaacgaa
    49621 gtccgcgccc ggtactgcca gcagcacgcc agtcatcccg cccaccgaga aagtgacgat
    49681 aaaaccgatg gtccacagca tcgcagaatg gaacacgata cggccctgat acatggtgaa
    49741 cagccagttg aagatcttca ccccggtcgg gatggcgata atcattgtgg tgataccaaa
    49801 gaaggcgttt acgttcgcgc ccgcacccat cgtaaagaag tggtgcagcc aaacgatgaa
    49861 cgacagcacg gtgatacaga cggttgccca taccagcgag gtataaccaa acagacgttt
    49921 acgcgagaag gttgccgcaa tttcggagaa cacaccgaaa acaggcagga tcaggatgta
    49981 aacttccggg tggccccagg cccaaatcag gttgatgtac atcatcatgt tgccacccat
    50041 atcgttggta aagaaatggg tgcccagata gcgatccagg gtcaacaacg cgacggtaac
    50101 cgtcagaatt gggaaggaag caataatcag tacgttcgcg cacagtgatg cccaggtaaa
    50161 tactggcatc ttgaacatgg tcatgcccgg tgcgcgcatc ttcagaatgg taacgaagaa
    50221 gttgataccg gtaagcgtcg tacctatacc ggatagctgg agactccata tccagtaatc
    50281 aacaccgact cccggactgt actctattcc cgatagcggt ggataggcca gccagccggt
    50341 ctgcgcaaat tcgcccacgc cgagagaaac gttaaccaga atcacaccaa caacagtaaa
    50401 ccagaagctt aagttgttga ggaacgggaa cgcaacgtca cgcgcgccga tctgcagcgg
    50461 aaccaccagg ttcatcagac cgataacgaa aggcatcgcc acgaagaaga tcataatcac
    50521 gccgtgcgcg gtgaagatct gatcgtagtg gtgaggtggc aggaagcccg cttcgcccgc
    50581 cgaggcaaga gcctgctggc tacgcatcat aatggcgtca gcaaaaccac gcagcaacat
    50641 cacaatcgcc acgatgatat acatgatacc gaggcgttta tggtcgacgg aggtcagcca
    50701 ctctttccac aggtaggtcc acttaccgaa gtaagtgatc aggccaacga gcgccagacc
    50761 tcccaaaata atgccagcga tcgtaaccat gacgataggt tcatggaacg ggactgcatc
    50821 aagtgataat tttccgaaca tctttattct tcctcaaccc ctttaatggg cggattccgc
    50881 gtggctcatg tccatgcctt ccataccttc gtgtgcgctg tgctcacctt ctggctgggt
    50941 catgtccatg ctcttaccgt gagccataaa cttgttaatc acatcagcaa acaagtctgg
    51001 tttcacgttg gagaaatatt ccacctggtt gtattcgcta ggcgcggcca gtttttcgaa
    51061 cgcagccatg tcagacatgg tgttcggcga ctgcttcgct tttgcgaccc actggtcgaa
    51121 ttcggcgcga tccggtgttg caatagcttt gaacttcatg cctgagaagc ccgggccgct
    51181 gtagctggcg gagataccgt cataagtacc gggttcgttg gcgatcagat gcaggcgagt
    51241 ctgcatacca gccatggcat aaatctggct acccagacgc ggaatgaaga aggagttcat
    51301 cacggagttg gaggtcactt tgaagtacac cggagtgttc gccgggaaag cgatttcatt
    51361 cacggtagca atgccctgtt ccgggtagat gaagaaccat ttccagtcca tggaaaccac
    51421 ttcgatggta atgggcttct cgtcgtgtgc cagcggcttg ctaggctcaa gagcgtgagt
    51481 ggttttccag gtcagtaccg caaggaagat gatgattaag ataggtaccg tccagaccac
    51541 agcttccact ttattggagt gtgaccagtt cgggctgtac ttagcatctt tattgctcgc
    51601 acgatacttc caggcgaaac caacagccat caagattgcg ggaataacga caatcaacat
    51661 caggccaaat gccgtcagta tcagtgaacg ttgctccaga ccaatctgtc ctttgggatc
    51721 taacagcgca gaattacagc cactgagcaa tacagtgcct gcaaataatg acaaccatcc
    51781 caaactttta ttgtatttcc tgagtctcat ttaacgacct caattccacg ggatctggtg
    51841 gcgtttaaag tgtgtgggca ttttatggga aggttacatt actgtaaaca tgattaaatt
    51901 cctgttactt ggatttgatt gtcaggtcac atatgttgca aaacatatca agttgatttt
    51961 aagaagctgt tgcaaaaggg cgttataacg aaaggggagt gcagaaggag tttggatggg
    52021 caattggtat aaccaatctg gaataaaata attgtttaac aaataattat cattatggtg
    52081 acaattgggt ggggggaaaa taaagactcg ttagctgaat cgtgtaagaa aaaatagaat
    52141 tcataaattg cgtcaataat ttccaaacgc taagccgcac aaaagaacaa atattattaa
    52201 gcgatactga tgtttattta gccgtaataa ttacggcgag tgattactac agctaaataa
    52261 tatttacaga ttacgtcaga tgcgtttttc gtagagccag ataatccagc aaaccaccaa
    52321 gcacgacgcc agaaagggcg actaaaaccc cgacttccag cagagcaggc aggaaagaga
    52381 agtgcgtcaa atccagcgcg tccatcgtca acagcagtaa ccacacggcc aacaggctga
    52441 cgcccgccgc cagtgtccac atggcaaagg cataacctgc cggatattcg gtacgggaga
    52501 taaagttgtc atttactcgt gtatattcaa gcgtctggcg gcaaaccagc agcaaaataa
    52561 gccccggtac ggcagcggcg acggagaata gatagaatgt cgaccagccg tgtgcttcaa
    52621 caaaccaacc cgccacgggg ccgacataaa ctcgtcctac agcggaaagc gctgagagca
    52681 gggcaaattg agtagcggaa aatgacttat tacacagcgt cattaacagc gcgacaaagg
    52741 ctgatgtgcc catcccgcca cagaggtttt cgaaaaagac ggctgcgccc atgctgtaga
    52801 gatgcttatc agtaatcgac agcagccagt aaccagcgtt agacgcacct tgtaaaatgc
    52861 cgaaaatcag cagcgcccgg aacagcgaca ggcgctgcat caaaatccca ccgtacaatg
    52921 cgcccacaat ggtcgccagt aagccaagcg ttttgttaac cacgcctact tcacccgcat
    52981 caaacccgac gccgcgaatc aaaaacgtgg ttgtcaggct catggcgaat gcgtcgccca
    53041 gcttatacag cacgataaga agcaaaataa gccaggcatt attgcgacca aagaaatctc
    53101 gcagaggtgc aacaaccgct tgttccagcg tttttggcac aggaatggtg tcggtcggtt
    53161 ctggtgcaag caacgtcgcg ataatacaag ggatcaacag cgccgccatc aaccagtaca
    53221 tgccctgcca gcccagccat ttatctgcca gccacagggc taagccgccg gaaaccagca
    53281 tccctaaacg gtaacccagc acgctgattg ccgcgcccgc accacgttct tctgccggga
    53341 gcacatcggt tttccacgca tcgaagacaa tatcctggga ggccgagcaa aaagcgatca
    53401 ccaccgccag tgccgccatc cagcggagtt gggtgccagg ttcgagaaaa cccatcgccg
    53461 caatggcgac taataacagg atttgcgtgg cgagcagcca accgcgccgc cgcccaaaaa
    53521 atgggggcgt gtagcggtcc atcagcggtg accagaggaa tttaaaaacg taagcctggc
    53581 ctaccagaga gaagaaacca atggttttga gatcaatatt ctcgaccgtc atccaagcct
    53641 gtaaggtacc ggatgtcagg gcgagcggta gcccggaagc aaaacccagg atcagcaata
    53701 tggctgaacg cggctgttga aaaatacgta aatattgact ggacatgggc gtctacaggc
    53761 ccggtttgca ccgggccaga gggcagaaat taacgcgcgt tctgcttgat gaattcgtga
    53821 atgctggtgt cctgagacat atcagcgatg gtatcggtca gcacactgtt aaccgcatcg
    53881 gcgatatttt tgttggaggc ctggaacgca ccttcaacgt tgtagctggc acgatagttt
    53941 ttggttattt tgttgccatt ctgcgcggta gcgatgatgg cgatatccgc tttggtagcg
    54001 atgttgtagc gcacgttgcc ctgggacacg tcagcataca gttggctaac gatgatttgc
    54061 agattaaccg ggccattcgg accaaccatg taaccacgcg cggtcatctg tttttccagc
    54121 acttcttgca gcaggaaacg cagatcgcgg gaggcggcca gggtaacgat ttgattatcg
    54181 cgggtgactt ttgccagcgc ctgatcggta cgctgatcgg caccattaat gcttacggtg
    54241 acgcccatca ggcttggatc ctgctgtggc agtgtaatcg tcggggaaac ttcaatagtt
    54301 gttggcggtt ttgcgcatcc tgcaagcata aacagagcaa ctaacgggaa gaggattttt
    54361 ttgaacatgt tcgggctctc agagactctt aagcgtgttt ggtaaaaatt cccgccatca
    54421 taacattgcc aacggcgagg ggaagtgggt aaggcatgta aattcatcat gttgacgaaa
    54481 taatcacccc tggtaaaaga aacgctgatg cgaggcctgt gtttcaatct ttaaatcagt
    54541 aaacttcata cgcttgacgg aaaaaccagg acgaaaccta aatatttgtt gttaagctgc
    54601 aatggaaacg gtaaaagcgg ctagtattta aagggatgga tgacatctca gcgttgtcgg
    54661 aggagatatt tcatgatgat acgtgagcgg atagaagaaa aattaagggc ggcgttccaa
    54721 cccgtattcc tcgaagtagt ggatgaaagc tatcgtcaca atgtcccagc cggctctgaa
    54781 agccatttta aagttgtgct ggtcagcgat cgttttacgg gtgaacgttt tctgaatcgt
    54841 catcgaatga tttacagtac tttagcggag gaactctcta ctaccgttca tgcgctggct
    54901 ctgcatactt acactattaa ggagtgggaa gggttgcagg acaccgtctt tgcctctcct
    54961 ccctgtcgtg gagcaggaag catcgcgtaa aaacgcattt gcaactgtcg gcgctttttc
    55021 agtatgttgc taaagatttt atgaaaaacg gcctgcgggc cgttttgttt tgtctggatt
    55081 ttgcgctttt tgcccagcat tcagacgaaa attgcccggg aattgtgaaa aaatacgcga
    55141 cagcgcgcaa taaccgttct cgactcataa aagtgatgcc gctataatgc cgcgtcctat
    55201 ttgaatgctt tcgggatgat tctggtaaca gggaatgtga ttgattataa gaacatcccg
    55261 gttccgcgaa gccaacaacc tgtgcttgcg gggtaagagt tgaccgagca ctgtgatttt
    55321 ttgaggtaac aagatgcaag tttcagttga aaccactcaa ggccttggcc gccgtgtaac
    55381 gattactatc gctgctgaca gcatcgagac cgctgttaaa agcgagctgg tcaacgttgc
    55441 gaaaaaagta cgtattgacg gcttccgcaa aggcaaagtg ccaatgaata tcgttgctca
    55501 gcgttatggc gcgtctgtac gccaggacgt tctgggtgac ctgatgagcc gtaacttcat
    55561 tgacgccatc attaaagaaa aaatcaatcc ggctggcgca ccgacttatg ttccgggcga
    55621 atacaagctg ggtgaagact tcacttactc tgtagagttt gaagtttatc cggaagttga
    55681 actgcagggt ctggaagcga tcgaagttga aaaaccgatc gttgaagtga ccgacgctga
    55741 cgttgacggc atgctggata ctctgcgtaa acagcaggcg acctggaaag aaaaagacag
    55801 cgctgttgaa gcagaagacc gcgtaaccat cgacttcacc ggttctgtag acagcgaaga
    55861 gttcgaaggc ggtaaagcgt ctgatttcgt actggcgatg ggccagggtc gtatgatccc
    55921 gggctttgaa gacggtatca aaggccacaa agctggcgaa gagttcacca tcgacgtgac
    55981 cttcccggaa gaataccacg cagaaaacct gaaaggtaaa gcagcgaaat tcgctatcaa
    56041 cctgaagaaa gttgaagagc gtgaactgcc ggaactgact gcagaattca tcaaacgttt
    56101 cggcgttgaa gatggttccg tagaaggtct gcgcgctgaa gtgcgtaaaa acatggagcg
    56161 cgagctgaag agcgccatcc gtaaccgcgt taagtctcag gcgatcgaag gtctggtaaa
    56221 agctaacgac atcgacgtac cggctgcgct gatcgacagc gaaatcgacg ttctgcgtcg
    56281 ccaggctgca cagcgtttcg gtggcaacga aaaacaagct ctggaactgc cgcgcgaact
    56341 gttcgaagaa caggctaaac gccgcgtagt tgttggcctg ctgctgggcg aagttatccg
    56401 caccaacgag ctgaaagctg acgaagagcg cgtgaaaggc ctgatcgaag agatggcttc
    56461 tgcgtacgaa gatccgaaag aagttatcga gttctacagc aaaaacaaag aactgatgga
    56521 caacatgcgc aatgttgctc tggaagaaca ggctgttgaa gctgtactgg cgaaagcgaa
    56581 agtgactgaa aaagaaacca ctttcaacga gctgatgaac cagcaggcgt aatttacgca
    56641 gcataacgcg ctaaattcgc acaaaggccc gtcaccgcca ggtggtgggc tttttttgtc
    56701 atgaattttg catggaaccg tgcgaaaagc ctctttcggt gttagcgtaa caacaaaaga
    56761 ttgttatgct tgaaatatgg tgatgccgta cccataacac agggactagc tgataatccg
    56821 tccataaggt tacaatcggt acagcaggtt ttttcaattt tatccaggag acggaaatgt
    56881 catacagcgg cgaacgagat aactttgcac cccatatggc gctggtgccg atggtcattg
    56941 aacagacctc acgcggtgag cgctcttttg atatctattc tcgtctactt aaggaacgcg
    57001 tcatttttct gactggccag gttgaagacc acatggctaa cctgattgtg gcgcagatgc
    57061 tgttcctgga agcggaaaac ccagaaaaag atatctatct gtacattaac tccccaggcg
    57121 gggtgatcac tgccgggatg tctatctatg acaccatgca gtttatcaag cctgatgtca
    57181 gcaccatctg tatgggccag gcggcctcga tgggcgcttt cttgctgacc gcaggggcaa
    57241 aaggtaaacg tttttgcctg ccgaattcgc gcgtgatgat tcaccaaccg ttgggcggct
    57301 accagggcca ggcgaccgat atcgaaattc atgcccgtga aattctgaaa gttaaagggc
    57361 gcatgaatga acttatggcg cttcatacgg gtcaatcatt agaacagatt gaacgtgata
    57421 ccgagcgcga tcgcttcctt tccgcccctg aagcggtgga atacggtctg gtcgattcga
    57481 ttctgaccca tcgtaattga tgccagaggc gcaactgtgc cgctatactt atccagggcg
    57541 gcacaacgct gtaagcggct tgcgcctgag aatggcattt gcgtcgtcgt gtgcggcaca
    57601 aagaacaaag aagaggtttt gacccatgac agataaacgc aaagatggct caggcaaatt
    57661 gctgtattgc tctttttgcg gcaaaagcca gcatgaagtg cgcaagctga ttgccggtcc
    57721 atccgtgtat atctgcgacg aatgtgttga tttatgtaac gacatcattc gcgaagagat
    57781 taaagaagtt gcaccgcatc gtgaacgcag tgcgctaccg acgccgcatg aaattcgcaa
    57841 ccacctggac gattacgtta tcggccagga acaggcgaaa aaagtgctgg cggtcgcggt
    57901 atacaaccat tacaaacgtc tgcgcaacgg cgataccagc aatggtgtcg agttgggcaa
    57961 aagtaacatt ctgctgatcg gtccgaccgg ttccggtaaa acgctgctgg ctgaaacgct
    58021 ggcgcgcctg ctggatgttc cgttcaccat ggccgacgcg actacactga ccgaagccgg
    58081 ttatgtgggt gaagacgttg aaaacatcat tcagaagctg ttgcagaaat gcgactacga
    58141 tgtccagaaa gcacagcgtg gtattgtcta catcgatgaa atcgacaaga tttctcgtaa
    58201 gtcggacaac ccgtctatta cccgtgacgt ttccggtgaa ggcgtacagc aggcactgtt
    58261 gaaactgatc gaaggtactg ttgctgccgt tccaccgcag ggcgggcgta agcatccgca
    58321 gcaggaattt ttgcaggttg atacctccaa gatcctgttt atttgtggcg gtgcgtttgc
    58381 cggtctggat aaagtgattt cccaccgtgt agaaaccggc tccggcattg gttttggcgc
    58441 gacggtaaaa gcgaagtccg acaaagcaag cgaaggcgag ctgctggcgc aggttgaacc
    58501 ggaagatctg atcaagtttg gtcttatccc tgagtttatt ggtcgtctgc cggttgtcgc
    58561 aacgttgaat gaactgagcg aagaagctct gattcagatc ctcaaagagc cgaaaaacgc
    58621 cctgaccaag cagtatcagg cgctgtttaa tctggaaggc gtggatctgg aattccgtga
    58681 cgaggcgctg gatgctatcg ctaagaaagc gatggcgcgt aaaaccggtg cccgtggcct
    58741 gcgttccatc gtagaagccg cactgctcga taccatgtac gatctgccgt ccatggaaga
    58801 tgtcgaaaaa gtggttatcg acgagtcggt aattgatggt caaagcaaac cgttgctgat
    58861 ttatggtaag ccggaagcgc aacaggcatc tggtgaataa ttaaccattc ccatacaatt
    58921 agttaaccaa aaagggggga ttttatctcc cctttaattt ttcctctatt ctcggcgttg
    58981 aatgtggggg aaacatcccc atatactgac gtacatgtta atagatggcg tgaagcacag
    59041 tcgtgtcatc tgattacctg gcggaaatta aactaagaga gagctctatg aatcctgagc
    59101 gttctgaacg cattgaaatc cccgtattgc cgctgcgcga tgtggtggtt tatccgcaca
    59161 tggtcattcc cttatttgtc gggcgggaaa aatctatccg ttgtctggaa gcggcgatgg
    59221 accatgataa aaaaattatg ctggtcgcgc agaaagaagc ttcaacggat gagccgggtg
    59281 taaacgatct tttcaccgtc gggaccgtgg cctctatatt gcagatgctg aaactgcctg
    59341 acggcaccgt caaagtgctg gtcgaggggt tacagcgcgc gcgtatttct gcgctctctg
    59401 acaatggcga acacttttct gcgaaggcgg agtatctgga gtcgccgacc attgatgagc
    59461 gggaacagga agtgctggtg cgtactgcaa tcagccagtt cgaaggctac atcaagctga
    59521 acaaaaaaat cccaccagaa gtgctgacgt cgctgaatag catcgacgat ccggcgcgtc
    59581 tggcggatac cattgctgca catatgccac tgaaactggc tgacaaacag tccgttctgg
    59641 agatgtccga cgttaacgaa cgtctggaat atctgatggc aatgatggaa tcggaaatcg
    59701 atctgctgca ggttgagaaa cgcattcgca accgcgttaa aaagcagatg gagaaatccc
    59761 agcgtgagta ctatctgaac gagcaaatga aagctattca gaaagaactc ggtgaaatgg
    59821 acgacgcgcc ggacgaaaac gaagccctga agcgcaaaat cgacgcggcg aagatgccga
    59881 aagaggcaaa agagaaagcg gaagcagagt tgcagaagct gaaaatgatg tctccgatgt
    59941 cggcagaagc gaccgtagtg cgtggttata tcgactggat ggtacaggta ccgtggaatg
    60001 cgcgcagcaa ggtcaaaaaa gacctgcgtc aggcgcagga aatccttgat accgaccatt
    60061 atggtctgga gcgcgtgaaa gatcgcatcc ttgagtacct tgcggttcaa agccgtgtca
    60121 acaaaatcaa gggaccgatc ctctgcctgg tagggccgcc gggggtaggt aaaacctctc
    60181 ttggtcagtc cattgccaaa gccaccgggc gtaaatatgt ccgtatggcg ctgggcggcg
    60241 tgcgtgatga agcggaaatc cgtggtcacc gccgtactta catcggttct atgccgggta
    60301 aactgatcca gaaaatggcg aaagtgggcg tgaaaaaccc gctgttcctg ctcgatgaga
    60361 tcgacaaaat gtcttctgac atgcgaggcg atccggcctc tgcactgctt gaagtgctgg
    60421 atccagagca gaacgtagcg ttcagcgacc actacctgga agtggattac gatctcagcg
    60481 acgtgatgtt tgtcgcgacg tcgaactcca tgaacattcc ggcaccgctg ctggatcgta
    60541 tggaagtgat tcgcctctcc ggttataccg aagatgaaaa actgaacatc gccaaacgtc
    60601 acctgctgcc gaagcagatt gaacgtaatg cactgaaaaa aggtgagctg accgtcgacg
    60661 atagcgccat tatcggcatt attcgttact acacccgtga ggcgggcgtg cgtggtctgg
    60721 agcgtgaaat ctccaaactg tgccgcaaag cggttaagca gttactgctc gataagtcat
    60781 taaaacatat cgaaattaac ggcgataacc tgcatgacta cctcggtgtt cagcgtttcg
    60841 actatggtcg cgctgataac gaaaactgtg tcggtcaggt aaccggtctg gcgtggacgg
    60901 aagtgggcgg tgacttgctg accattgaaa ccgcatgtgt tccgggtaaa ggcaaactga
    60961 cctataccgg ttcgctcggc gaagtgatgc aggagtctat tcaggcggcg ttaacggtgg
    61021 ttcgtgcgcg tgcggaaaaa ctggggatca accctgattt ttacgaaaaa cgtgacatcc
    61081 acgtccacgt accggaaggt gcgacgccga aagatggtcc gagtgccggt attgctatgt
    61141 gcaccgcgct ggtttcttgc ctgaccggta acccggttcg tgccgatgtg gcaatgaccg
    61201 gtgagatcac tctgcgtggt caggtactgc cgatcggtgg tttgaaagaa aaactcctgg
    61261 cagcgcatcg cggcgggatt aaaacagtgc taattccgtt cgaaaataaa cgcgatctgg
    61321 aagagattcc tgacaacgta attgccgatc tggacattca tcctgtgaag cgcattgagg
    61381 aagttctgac tctggcgctg caaaatgaac cgtctggcat gcaggttgtg actgcaaaat
    61441 agtgacctcg cgcaaaatgc actaataaaa acagggctgg caggctaatt cgggcttgcc
    61501 agcctttttt tgtctcgcta agttagatgg cggatcgggc ttgcccttat taaggggtgt
    61561 tgtaagggga tggctggcct gatataactg ctgcgcgttc gtaccttgaa ggattcaagt
    61621 gcgatataaa ttataaagag gaagagaaga gtgaataaat ctcaattgat cgacaagatt
    61681 gctgcagggg ctgatatctc taaagctgcg gctggccgtg cgttagatgc tattattgct
    61741 tccgtaactg aatctctgaa agaaggggat gatgtagcac tggtaggttt tggtactttt
    61801 gccgttaaag agcgtgctgc ccgtactggc cgcaacccgc agaccggtaa agagatcacc
    61861 atcgctgctg ctaaagtacc gagcttccgt gcaggtaaag cactgaaaga cgcggtaaac
    61921 taagcgttgt ccccggtggg gatgtgacga agttcaaggg cgcatctact gatgtgcctt
    61981 ttttatttgt attcggtgac tttctgcgtc ttgtgggctg acaattgccc ccgtttcttg
    62041 tcacaatagg cctttgcgcg catcgatacg ttgcgtgagg tacacagtca tctacagcgg
    62101 agtgttgtta caccatgatg gacagcttac gcacggctgc aaacagtctc gtgctcaaga
    62161 ttattttcgg tatcattatc gtgtcgttca tattgaccgg cgtgagtggt tacctgattg
    62221 gcggaggcaa taactacgcc gcaaaagtga atgaccagga aatcagccgt gggcagttcg
    62281 agaacgcctt taacagcgag cgtaatcgca tgcagcaaca gctgggcgat caatactccg
    62341 agctggcagc gaatgaaggc tatatgaaaa ccctgcgtca acaggtgctg aatcgtctga
    62401 tcgacgaggc gctgttggat cagtacgctc gtgagctgaa actgggtatc agtgatgagc
    62461 aggttaaaca ggcgattttc gcgaccccag ccttccaggt tgacggcaaa tttgataaca
    62521 gccgctataa cggtatcctc aaccagatgg ggatgaccgc cgatcagtac gcccaggcgc
    62581 tgcgtaacca gctcactacc caacagctga ttaacggtgt tgccggtacc gattttatgc
    62641 tgaaaggtga aaccgacgag ctggcggcac tggtcgcgca acaacgcgtg gtgcgtgagg
    62701 cgactatcga tgttaacgcg ctggcggcga agcagcctgt gaccgaacag gagattgcca
    62761 gctactacga acaaaacaaa aacaatttca tgacgccgga acaattccgc gtgagttaca
    62821 tcaagctgga tgccgcaacg atgcagcaac cggttagcga tgcggatatc cagagctact
    62881 acgatcagca tcaggatcaa ttcacccagc cgcagcgtac ccgctacagc atcatccaga
    62941 ccaaaactga agatgaagcg aaagcggtac ttgatgagct gaataaaggc ggtgattttg
    63001 ctgcattagc caaagaaaaa tctgccgata ttatctctgc ccgtaacggc ggcgatatgg
    63061 gttggttaga agatgccact atccccgacg aactgaaaaa tgctggtctg aaagaaaaag
    63121 gccaactgtc tggtgtcatc aaatcttcgg tcggtttcct gattgtacgt ctggacgaca
    63181 ttcagccagc gaaagtgaaa tcgttagacg aagtacgtga cgacattgcg gcgaaagtga
    63241 aacacgaaaa agccctcgat gcgtactatg cgctgcagca gaaagtgagc gatgcggcaa
    63301 gcaacgacac cgagtctctg gccggtgcag agcaagctgc cggcgttaaa gccactcaga
    63361 cgggttggtt cagcaaagat aacctgccgg aagagttgaa cttcaagccg gttgccgacg
    63421 ctatctttaa cggcggtctg gtaggtgaaa acggcgcgcc gggcatcaac tctgacatca
    63481 tcaccgtaga cggcgaccgt gcattcgtgc tgcgcatcag cgagcacaaa ccggaagcgg
    63541 tgaaaccgtt ggcagatgtt caggaacaag ttaaggcact ggttcagcac aacaaagctg
    63601 aacaacaggc gaaagtggat gctgagaaac tgctggttga tttgaaagcc ggcaaaggtg
    63661 cggaagctat gcaggctgcc ggtctgaaat ttggcgagcc gaaaacctta agccgttccg
    63721 gtcgtgaccc gattagccag gcggcgtttg cactgccact gccagcgaaa gacaaaccga
    63781 gctacggtat ggcgaccgat atgcaaggta atgtggttct gctggcgctg gatgaagtga
    63841 aacaaggttc aatgccggaa gatcagaaaa aagcgatggt gcagggtatc acccagaaca
    63901 acgcacaaat cgtctttgaa gctctgatga gtaacctgcg taaagaggcg aaaatcaaaa
    63961 ttggcgatgc gctggaacag caataatcct gaagccgcct cgcaaaaaaa tgcgttgcag
    64021 ctgtaacaag aaaaggtcgc tttcgcggcc ttttccattt ctgaacattg ccatttgttt
    64081 actgttttca ctgccgttaa ggtgattcca ctgttaacaa acaaggagaa aacagtatga
    64141 aacacggaat taaagcactg ctcattaccc tgtccctggc ctgtgccgga atgtctcata
    64201 gcgcgctggc ggcagcttct gtggcgaaac cgacggcggt agaaaccaaa gcggaagctc
    64261 ctgcagcaca aagtaaagca gcagtaccgg cgaaagccag tgacgaagaa ggcacccggg
    64321 tcagcattaa taatgccagc gcggaagagc tagcccgcgc gatgaatggc gttggcctga
    64381 agaaagcgca ggcgattgtc agttatcgcg aagagtacgg tccgtttaaa acagtggagg
    64441 atctaaagca ggtgccgggg atgggcaatt cgctggtgga acgtaatctg gcggtattaa
    64501 ccctgtaatt aatttgcata gtggcaattt tttgccagac tgaagaggtc ataccagtta
    64561 tgacctctgt acttataaca acaacgtaag gttattgcgc tatgcaaaca caaataaaag
    64621 ttcgtggata tcatctcgac gtttaccagc acgtcaacaa cgcccgctac cttgaatttc
    64681 tcgaagaagc ccgctgggat gggttggaaa atagcgacag ttttcagtgg atgacggccc
    64741 ataacatcgc cttcgtcgtg gtcaatatca atattaacta tcgtcgccca gcggtattaa
    64801 gtgacctgtt aactattacc agtcagttgc agcaattaaa cggtaaaagc ggcatcttaa
    64861 gccaggtcat tacactggag ccggaagggc aggtggtagc ggatgcgctt attacgtttg
    64921 tttgtattga tcttaaaacg cagaaagcat tagctctgga aggggaattg cgcgaaaagc
    64981 tggagcagat ggttaagtaa acgttttgtg gtgccggatg ctcaagccgc atccggcgac
    65041 acccggaata attacctcaa cccggttttc tgcttcatcg ctgccatcac cgtcggttta
    65101 tcggccagat aatgattcaa accgttggcg cgtaaattac atgccgcaca atgaccgcaa
    65161 ccgtcgcctt taatgccgtt atagcaggtc aacgtttcgt tacggactaa atccagtttg
    65221 ccgtaataat ctgccagcgc ccaggtttcc gctttatcaa tccacatcag cggcgtttca
    65281 aaacgaatat ctttctccat gcccaaactg acggcatggt ttagtgcttt cacaaactca
    65341 tcgcggcaat ccgggtagcc ggagaaatcc gtttcacaga cgccggtaat tacggcttct
    65401 gcttttacct gatacgcata tattgccgcc agcgtcagga acaaaatatt acgccctggg
    65461 acaaacgtat tcgggatgcc atcggcttca ggttcataat caggcaccgg aatgctgtca
    65521 cgtgtcaggc tgctgaccgc cagctcgttg agcagcgtta catcaagaac cttatgcgcg
    65581 cgtgctccca gtttcagcgc cagttcgcgt gccacgtcga tttctgcgcg atgtcgctga
    65641 ccgtaatcga acgtcacgca atggacttca tcatattgtt gtaatgcctg caccagacag
    65701 gtggtggaat cctggcctcc actgaacaca acgacagcac gtttcataga taatcctgct
    65761 tgaacaataa aagcgttatg gtaacgcctg cgattaaccc ggaccagcta ttcattgcga
    65821 cggcgcggga agccaggctt cggtaaattc aaaccagccg cgcggtgtca gtcgcacacc
    65881 gttcacgccg ggaggggcac taatgcgata gtgatagttg aacagcggcg tcagcgtcgc
    65941 gtctgccatt aactggctaa aaaccgcttt cagggcatta aatcggtttt cctcatcagg
    66001 cattacttgc accgcgtcca gcgtcgattg cagatgtgca tatgctggag cgtcgaaaac
    66061 atgtggccat agtggatcgc aacgtagcca ttgctccaga gtatattccg gtgcttcgcc
    66121 aattaatctg tcgcccatca tgaggtctgc gtgtgccagt agggtcgtgt cgtcccagtt
    66181 ttttgcgtta tgaaaaataa ttgtgagttc acagccttcc gctgccagtg tcgcctgtag
    66241 gcgttctgcc atggtatgaa gttctatcgg taggtgataa accagcgtca aggttttcgg
    66301 tagtttgact tcatccggta cttgccagtg cggaatagtc cagcctggca gtaatgcatg
    66361 actggcggtg atcaggtttt ctccgacttc taacgtttgt aataaaccgg attgatgaat
    66421 aatggagatc acttttcgcg cctgccagag ggagagtcgg ggacttttgc gcaacgttaa
    66481 atagcaaaaa cctaaactga tgccactact gacctggctg acccgttgca gctcctccgg
    66541 tttgccgatg gtgatttgca cgggatgccg acaactggtt cccaaatctt tttcgaaaag
    66601 cggcggagtt atccagtact caaccgcttt aagcagcgga tgacgcaaat ggtaataatc
    66661 atggctctcc aggcgcacca gctccgctgt gaattgtgtt aagcgaaaag gaccagtgcc
    66721 gatcagtggg aattgcggat gcgccagatg gctgcaatag ctcgccagcc ggtgcgcaag
    66781 ccagtaatcg gggcgatgta aaaagaaggt cagacactgc ggatgggtga cttcaatacg
    66841 cttcacgcta ataaataatt gatccagtgc tggcagttgt aacagcatca ataatcgctg
    66901 gtgtaagtgt gaggctttta ctgcatcgcc gttatgccag tgtagggttg aacgaagata
    66961 aaagtcccag cgtaacccgt cagtagaggt ttcccagtga tgcgctaaat cgccaatcgg
    67021 gcgctgagta ttattatcga agcgggtcag gccggaaaat atctgcccgg cgagatgctg
    67081 ctcggcacgg ccgggcaaaa agcctggttg tagcggttcg agcgggcgat agtagggaat
    67141 acgcaacgtg ggtgtatcgt tttgccattg tccgcccata aacggctgta acagagtgcg
    67201 tagctcacct agggccagtt gcgccagctc cagcacatct tgctgctttc cggtttccag
    67261 tgcctgttcc atcatcgcat tgcgtagcga ttccggcgtg accagaaagc gtaattgtcc
    67321 gcgctttccg cgtcctgact gcgcctgcca ctccagccat cccgcctcct gtgcctgacg
    67381 caacagcgta cgaacatggc gttcgctgca aaaacagcgt tcggccagtt cgctgacggt
    67441 gacggtttgc ggctttccgg ctgaaggttg ccacagacgt tgatactggt taagacggtt
    67501 gagcaatcgc atataaaccc ggaacaatat tatttaacta ttcactatta cttccgtata
    67561 tatcaggtga tactcaatct ccattaaccg tgtcacagag tggagaaaga aatggctcgt
    67621 ctggcagcat ttgatatgga tggcacttta ttgatgcctg accatcattt aggtgagaaa
    67681 accctctcta ctttggcgcg actgcgtgaa cgcgacatta ccctcacttt tgccacgggg
    67741 cgtcatgcgc tggagatgca gcatattctc ggggcgctat cgctggatgc atatttgatt
    67801 accggcaacg gaacgcgcgt gcattctctg gaaggtgaac ttttacatcg tgatgattta
    67861 cctgcggatg tcgcggagct ggtgctgtat cagcaatggg atacccgagc cagcatgcat
    67921 atcttcaatg acgacggttg gtttaccggg aaagagatcc ctgcgttgtt gcaggcattt
    67981 gtctatagcg gttttcgtta tcagataatc gatgtcaaaa aaatgccact cggcagcgtc
    68041 accaagatct gcttctgtgg cgatcacgac gatcttacac gcttgcagat ccagctatac
    68101 gaagcattag gcgagcgtgc acatttgtgt ttttccgcca cggattgcct cgaagtgctg
    68161 ccggtgggct gcaataaagg cgctgcattg acggtgctga cccaacatct aggtttatcg
    68221 ttgcgcgatt gcatggcctt tggtgatgcg atgaacgatc gcgaaatgtt aggcagcgtc
    68281 ggtagcgggt ttattatggg caatgcgatg ccgcaactgc gcgcggagct cccgcattta
    68341 ccggtgattg gacattgccg aaattaggct gtctctcact atttgacgca ctgactggac
    68401 tatccacatc taccttattc ccccgaataa tgagatccct tccagcaccg ggcaattgcc
    68461 cggttttttt tgcgttgaat ttgtcatttt gtgccgtggt gtttaaaccg cacagaataa
    68521 attgtcgtga tttcaccttt aaaatagaat taaaagagaa aaaaattctc tgtggaaggg
    68581 ctatgttaga taaaattgac cgtaagctgc tggccttact gcagcaggat tgcaccctct
    68641 ctttgcaggc actggctgaa gccgttaatc tgacaaccac cccttgctgg aagcgcctga
    68701 aacggctgga ggacgacggt atccttatcg gcaaagtcgc cctgctggat ccggaaaaaa
    68761 taggcctcgg cctgaccgcc tttgtgctga taaaaacgca acatcacagt agcgaatggt
    68821 attgccgctt tgtcacggtg gttaccgaaa tgccagaagt gctggggttc tggcgcatgg
    68881 ctggtgaata cgattatctg atgcgcgtcc aggttgccga catgaaacgc tacgacgagt
    68941 tttataagcg tctggtaaac agcgtgccgg ggctgtcgga cgtcacttcc agcttcgcga
    69001 tggaacagat taaatacacc acttctttac ccatcgaata aatatccaga atcaggtcag
    69061 gacacaacgc gtgcgattat ttgctcaatt aagctggtat ttccgtcggg aatggcgtcg
    69121 ctatctcggg gctgtcgcct tgcttgtcat tatcgcgatg ctgcaactgg ttccgccaaa
    69181 agtggttggt attgttgtcg atggcgtgac agaacaacac tttactaccg ggcagatcct
    69241 gatgtggatc gccaccatgg tgctgattgc cgttgtggtt tatttcctgc gttacgtctg
    69301 gcgggtattg ctgtttggtg cgtcttatca actggctgtt gaactgcgtg aagattatta
    69361 ccgtcagcta agccggcagc atcctgagtt ttacctgcgt catcgcacgg gtgacctcat
    69421 ggctcgtgcg accaatgacg tcgatcgcgt cgtgtttgca gccggagaag gggtgctgac
    69481 gctggtggat tcactggtga tgggctgcgc tgtgttgatt atgatgtcta cgcaaattag
    69541 ctggcagttg accttatttg ccctgttgcc gatgccagtg atggcgatca tgatcaagcg
    69601 caacggcgat gccttgcatg aacgctttaa gctggcacag gcggcgtttt ctagtcttaa
    69661 tgaccgcacc caggaaagcc tcaccagtat ccgcatgatc aaagcctttg gtctggaaga
    69721 tcgccagtcg gcgttatttg ccgcggatgc cgaagatacc ggcaaaaaaa acatgcgggt
    69781 ggcgcgtatt gatgctcgtt tcgacccgac catctatatc gcgattggta tggcgaattt
    69841 gctggcgatt ggcggcggta gttggatggt ggtgcagggc agtttaacgc tgggccagct
    69901 caccagtttt atgatgtatt taggtctgat gatttggcca atgctggcgc tggcatggat
    69961 gtttaacatt gtggaacgtg gtagtgctgc atacagccgt attcgcgcga tgctggcgga
    70021 agcgccggtg gtgaacgatg gtagcgaacc cgtgccggaa gggcgtggcg aactggatgt
    70081 aaatattcac cagttcacgt atccgcagac tgaccatcct gcgctggaaa acgtcaattt
    70141 cgccctgaaa cccggtcaga tgctgggtat ctgcgggccg actggttccg gcaaaagtac
    70201 cctgttgtcg ctcattcagc gtcatttcga cgtcagcgag ggggatattc gctttcatga
    70261 tattcctctg acgaagttac aactcgatag ctggcgtagc cgcctggcgg tagttagcca
    70321 gacaccgttc cttttttccg acaccgtagc gaacaacatc gcgttgggtt gcccgaatgc
    70381 cacccagcaa gagattgagc atgtcgcgcg gttagccagc gtacatgacg atattttgcg
    70441 tctaccgcaa ggttacgata cagaagtggg cgagcgcggc gtgatgcttt ccggtgggca
    70501 aaaacagcgt atctccattg ctcgagcgtt attagtcaac gcggaaatcc tcatccttga
    70561 tgatgcgctt tcggcggtgg acggacgcac agagcaccag atcctgcata acctgcgtca
    70621 gtggggacag ggaagaacgg taatcatcag tgcccatcgc ctttcggcac tgacggaagc
    70681 cagtgaaaat attgtgatgc agcacggaca tatcgcccag cgtggcaatc atgatgagct
    70741 ggcgcaacaa agcggctggt atcgcgatat gtatcgctat caacaactgg aggcggcgct
    70801 cgacgacgct ccggaaattc gcgaggaggc cgtcgatgcg tagttttagc caactgtggc
    70861 cgactctcaa gcgtctgtta gcgtacggtt cgccgtggcg taaaccgctg gggattgcgg
    70921 tcctgatgat gtgggttgcg gcagcggcag aagtcagtgg gccgctgctt atcagctatt
    70981 ttatcgacaa tatggtagcg aaaaataacc tgccgttgaa aatggttgca gggctggctg
    71041 cggcgtatgt tgggctacaa ctgtttgccg ccgggctaca ttacgcgcag tcgctgctgt
    71101 ttaatcgggc ggcagtaggc gtagtgcaac agttgcgtac cgacgtgatg gatgccgcat
    71161 tgcgccagcc attaagcgag tttgataccc aacccgtcgg gcaggtgatt tcccgcgtca
    71221 ctaatgacac tgaagtgatc cgcgatctct acgttaccgt agtggcaact gtcctgcgta
    71281 gcgccgcact ggtgggcgcg atgctggtgg cgatgttcag cctcgactgg cgaatggcgc
    71341 tggtggcgat aatgattttc ccggtggtgc tggtggtaat ggtgatatac cagcgttaca
    71401 gcacgccgat tgtccgtcgt gtgcgcgcct atctggcgga tatcaacgac ggctttaacg
    71461 aaatcatcaa tggcatgagc gttatccagc agttccgtca gcaggcgcga tttggcgaac
    71521 gtatggggga ggccagtcgt tcacactata tggccaggat gcaaaccctg cgcctcgacg
    71581 gttttctgct gcgtccgctg ctgagtctgt tttcatcgct cattctttgt ggcttgttga
    71641 tgctgtttgg cttctccgct agcggcacca ttgaagtagg cgtgctgtat gcgtttatca
    71701 gctatcttgg gcgacttaac gaacccttaa tcgaactgac cacgcaacag gcgatgctgc
    71761 aacaggctgt tgttgctggt gagcgcgtgt ttgaactgat ggacggaccg cgccagcaat
    71821 atggcaatga tgatcgcccg ttacagagtg gcaccatcga agtcgataac gtgtcatttg
    71881 cttatcgcga tgacaatctg gtgctaaaga acattaatct ctctgtgcct tcgcgtaact
    71941 ttgtggcact ggtcgggcat accggcagtg gcaaaagcac cctcgccagt ttattgatgg
    72001 gctattaccc gctaacggaa ggtgagattc gccttgatgg tcgtccatta agttcgctaa
    72061 gtcacagcgc gctgcgccag ggcgtggcaa tggtgcagca agatccggtg gtgctggcgg
    72121 ataccttcct cgccaacgtg acgctggggc gtgatatctc cgaagaacgc gtctggcagg
    72181 cgctggaaac cgtgcaactg gcggagctgg cgcgcagcat gagtgatggt atttatacgc
    72241 cgctgggcga gcaggggaat aatctctccg tcgggcaaaa gcaactgctg gcactggcac
    72301 gcgtgttggt ggagacgccg caaatcctga tccttgatga ggcaaccgcc agcattgact
    72361 ccggtactga acaggcgatt caacatgctc tggcggctgt gcgtgaacat accacgctgg
    72421 tggtgattgc tcaccgctta tcgaccattg ttgatgccga caccattctg gtgcttcatc
    72481 gtgggcaagc cgtggagcag ggcactcacc agcaactgct ggcggcccag ggacgctact
    72541 ggcagatgta tcaactgcaa cttgcgggcg aagagctggc agccagcgtg cgtgaagaag
    72601 aatcattgag cgcctgaata gcgcaatatt tcatcgttgg tgcaaaaatg taacgcactg
    72661 tgcactgtca tagtgcgttt tcattttcaa tcttcttaac ttcctgctct ctttctcgtt
    72721 tttcatttct ggcacaccgc ttgcaatacc ttcttcgtgt agcagaacca ttaccgaatt
    72781 ctgactggag gggatctatg aagctggtga ccgtgataat caaaccattc aagctggaag
    72841 acgttcgtga agcgttatct tccattggta ttcagggcct gaccgtcacc gaagtgaaag
    72901 gtttcgggcg tcagaaaggg catgccgagc tgtaccgggg ggcggaatac agcgtcaact
    72961 tcctgccaaa agtaaaaatt gatgtggcaa ttgctgatga ccaactcgat gaagtgatcg
    73021 atatcgtcag taaggcggct tacaccggaa aaattggcga cggcaaaatc ttcgtcgctg
    73081 aattgcaacg cgtcattcgt attcgtaccg gcgaagccga cgaagcggcg ctgtaatctc
    73141 tggcacacag caacaggaac gaaaaatgaa gatagcgacg ataaaaactg ggcttgcttc
    73201 actggcgatg ctgccgggac tggtaatggc tgcacctgcg gtggccgata aagccgacaa
    73261 tgcgtttatg atgatttgta ctgcgctggt gctgtttatg actattccgg ggattgccct
    73321 gttttacggt gggttgattc gcggcaaaaa cgtgctgtcg atgctgatgc aggtgacggt
    73381 gacgtttgca ctggtctgta ttctctgggt ggtttacggt tactcgctgg cgtttggtga
    73441 gggtaacaac ttcttcggca acgttaactg gttgatgctg aaaaacatcg aactgacggc
    73501 ggtgatgggc agcatttatc agtatatcca cgtagcgttt cagggatcgt ttgcctgcat
    73561 taccgtaggc ttgatagttg gggcgctggc ggaacgaatc cgcttctcag ctgtgttgat
    73621 tttcgtggtg gtatggctga cgctctctta cattccgatt gcgcatatgg tgtggggcgg
    73681 cggtttgctg gcttctcacg gtgcactgga tttcgcgggt ggcacagtgg tgcacattaa
    73741 cgccgcaatc gccggtctgg tgggcgcgta tctgataggc aaacgcgtgg gcttcggtaa
    73801 agaggcgttt aaaccgcaca acctgccgat ggtcttcacc gggactgcca ttctctatat
    73861 cggttggttt ggctttaacg ccgggtcagc gggcacggcg aatgaaatcg cggcactggc
    73921 atttgtgaat actgtggtcg caacggcggc ggcaattctt ggctggatct tcggtgaatg
    73981 ggcgctgcgt ggtaagcctt cactgctggg ggcgtgttct ggcgcgattg ccggtctggt
    74041 cggcgtgacg ccagcgtgcg gctacattgg ggttggcggc gcgttgatta tcggcgtggt
    74101 agctggtctg gcgggcttgt ggggcgttac catgctcaaa cgcttgctgc gggtggatga
    74161 tccctgcgat gtcttcggtg tgcacggcgt ttgtggcatt gtcggctgta tcatgaccgg
    74221 gatttttgcc gccagctcgc tgggcggcgt gggcttcgct gaaggtgtga cgatgggtca
    74281 tcagttgctg gtacagctgg aaagcatcgc cattacgatc gtctggtccg gtgtggtggc
    74341 atttattggc tacaaattgg cggatctgac ggttggtctg cgtgtaccgg aagagcagga
    74401 gctagaaggg ctggatgtca acagtcacgg cgagaatgcc tataacgcgt aacaagcact
    74461 gcaaaaaaca gccggacggt tttcacctcc ggctattttt ttaattgtga ttacgcatca
    74521 ccccttcctg aacggtcgag gcaaccagta cgccgtcctg ggtataaaac tcaccgcgca
    74581 caaagccacg tgcgctggac gccgaggtgc tctccacgct atacagcagc cattcattca
    74641 aattaaacgg gcgatggaac cacatggaat ggtcaatggt ggcaatctga atccccggtt
    74701 cgagaaaacc gatgccgtgc ggctgtagag ctaccggcag gaagttaaga tcagaagcgt
    74761 aaccgagcag atactgatga acgcgcaggt catccggcac gctaccattt gcgcggatcc
    74821 acacctgacg atgtggttct gcgacgtgac ctttcagtgg gttatgaaac tccaccggac
    74881 ggacttccag cggacgatcg cagatgaatt tatctttaag cactggcggc agcaggtgcg
    74941 ccagcgattg ggcgatttgc gtttccgaag gcaggccatc aggcgctggc gcggacggca
    75001 ttgttttttg atgttcgaaa cccgcttctg gtgcctggaa agaggccgtc atataaaaaa
    75061 tcggtttgcc gttttgaata gcagcaaccc ggcgggcgct gaagctgtta ccatcacgca
    75121 gcgtttcgac atcataaata atcggtttct tactatcgcc tgggcgaaga aagtagctgt
    75181 gaaacgaatg taccagccgc tcttccggga cggtctcttt tgcagcatac aaggcctgac
    75241 ccacgacctg gccgccaaac acctggcgta aacctaaatc ttcactctgg ccgcgaaaga
    75301 gtcctgcctc aattttttcc agatttaaca atgccagtaa attttttagc gcctgactca
    75361 tataactctc cagtaacaaa gctgccgcag caagccaaag tgagttgagt ataacgcaaa
    75421 tttgccactg gtccgatggg tgcaataatc tgaattacgg gctaattaca ggcagaaatg
    75481 cgtgatgtgt gccacactta ttgacgtta
//
LOCUS       DAJTYD010000003        66724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTYD010000003 DAJTYD010000000
VERSION     DAJTYD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.003"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1164
                     /gene="cas3"
                     /locus_tag="OO585_000169"
     CDS             <1..1164
                     /gene="cas3"
                     /locus_tag="OO585_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HCR6627885.1"
                     /translation="LLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQEC
                     IARIESAVSQGKCIAWIRNSVDDAIQVYRQLLARGVIPASSLSLFHSRFAFSDRQRIE
                     TETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHI
                     RDINGQLKRDGKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQR
                     VLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKYVLNFR
                     LGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWW
                     KKHRDEFSLLEGDAFRQWCVEQRQDPEMANVILVTDDESCGYSAREGLIGKVG"
     gene            1262..2824
                     /gene="casA"
                     /locus_tag="OO585_000170"
     CDS             1262..2824
                     /gene="casA"
                     /locus_tag="OO585_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCR6627886.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVCLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            2821..3368
                     /gene="casB"
                     /locus_tag="OO585_000171"
                     /pseudo
     CDS             2821..3368
                     /gene="casB"
                     /locus_tag="OO585_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            3383..4461
                     /gene="cas7e"
                     /locus_tag="OO585_000172"
                     /pseudo
     CDS             3383..4461
                     /gene="cas7e"
                     /locus_tag="OO585_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            4472..5218
                     /gene="cas5e"
                     /locus_tag="OO585_000173"
     CDS             4472..5218
                     /gene="cas5e"
                     /locus_tag="OO585_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6627887.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            5200..5850
                     /gene="cas6e"
                     /locus_tag="OO585_000174"
     CDS             5200..5850
                     /gene="cas6e"
                     /locus_tag="OO585_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6627888.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            5847..6767
                     /gene="cas1e"
                     /locus_tag="OO585_000175"
     CDS             5847..6767
                     /gene="cas1e"
                     /locus_tag="OO585_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR6627889.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            6767..7060
                     /gene="cas2"
                     /locus_tag="OO585_000176"
     CDS             6767..7060
                     /gene="cas2"
                     /locus_tag="OO585_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6627890.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(7267..8304)
                     /gene="iap"
                     /locus_tag="OO585_000177"
     CDS             complement(7267..8304)
                     /gene="iap"
                     /locus_tag="OO585_000177"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6627891.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            8556..9464
                     /gene="cysD"
                     /locus_tag="OO585_000178"
     CDS             8556..9464
                     /gene="cysD"
                     /locus_tag="OO585_000178"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6627892.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            9466..10893
                     /gene="cysN"
                     /locus_tag="OO585_000179"
     CDS             9466..10893
                     /gene="cysN"
                     /locus_tag="OO585_000179"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6627893.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            10893..11498
                     /gene="cysC"
                     /locus_tag="OO585_000180"
     CDS             10893..11498
                     /gene="cysC"
                     /locus_tag="OO585_000180"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6627894.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            11548..11871
                     /locus_tag="OO585_000181"
     CDS             11548..11871
                     /locus_tag="OO585_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6627895.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            12065..12376
                     /gene="ftsB"
                     /locus_tag="OO585_000182"
     CDS             12065..12376
                     /gene="ftsB"
                     /locus_tag="OO585_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6627896.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            12395..13105
                     /gene="ispD"
                     /locus_tag="OO585_000183"
     CDS             12395..13105
                     /gene="ispD"
                     /locus_tag="OO585_000183"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6627897.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            13105..13584
                     /gene="ispF"
                     /locus_tag="OO585_000184"
     CDS             13105..13584
                     /gene="ispF"
                     /locus_tag="OO585_000184"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6627898.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            13581..14630
                     /gene="truD"
                     /locus_tag="OO585_000185"
     CDS             13581..14630
                     /gene="truD"
                     /locus_tag="OO585_000185"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6627899.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            14611..15372
                     /gene="surE"
                     /locus_tag="OO585_000186"
     CDS             14611..15372
                     /gene="surE"
                     /locus_tag="OO585_000186"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6627900.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            15366..15992
                     /gene="pcm"
                     /locus_tag="OO585_000187"
     CDS             15366..15992
                     /gene="pcm"
                     /locus_tag="OO585_000187"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6627901.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            16132..17223
                     /gene="nlpD"
                     /locus_tag="OO585_000188"
     CDS             16132..17223
                     /gene="nlpD"
                     /locus_tag="OO585_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6627902.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            17286..18278
                     /gene="rpoS"
                     /locus_tag="OO585_000189"
     CDS             17286..18278
                     /gene="rpoS"
                     /locus_tag="OO585_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6627903.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(18372..19736)
                     /locus_tag="OO585_000190"
     CDS             complement(18372..19736)
                     /locus_tag="OO585_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6627904.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(19825..20600)
                     /locus_tag="OO585_000191"
                     /pseudo
     CDS             complement(19825..20600)
                     /locus_tag="OO585_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
     gene            complement(20605..21243)
                     /locus_tag="OO585_000192"
     CDS             complement(20605..21243)
                     /locus_tag="OO585_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6627905.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTVLSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(21240..22502)
                     /locus_tag="OO585_000193"
     CDS             complement(21240..22502)
                     /locus_tag="OO585_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6627906.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(22499..23407)
                     /locus_tag="OO585_000194"
     CDS             complement(22499..23407)
                     /locus_tag="OO585_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6627907.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            23603..24370
                     /locus_tag="OO585_000195"
     CDS             23603..24370
                     /locus_tag="OO585_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6627908.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(24421..25077)
                     /gene="pphB"
                     /locus_tag="OO585_000196"
     CDS             complement(24421..25077)
                     /gene="pphB"
                     /locus_tag="OO585_000196"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR6627909.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(25183..27744)
                     /gene="mutS"
                     /locus_tag="OO585_000197"
     CDS             complement(25183..27744)
                     /gene="mutS"
                     /locus_tag="OO585_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6627910.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            27820..27954
                     /locus_tag="OO585_000198"
     CDS             27820..27954
                     /locus_tag="OO585_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6627911.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            28031..28384
                     /locus_tag="OO585_000199"
     CDS             28031..28384
                     /locus_tag="OO585_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6627912.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(28421..30499)
                     /gene="flhA"
                     /locus_tag="OO585_000200"
                     /pseudo
     CDS             complement(28421..30499)
                     /gene="flhA"
                     /locus_tag="OO585_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(30573..31583)
                     /gene="hypE"
                     /locus_tag="OO585_000201"
     CDS             complement(30573..31583)
                     /gene="hypE"
                     /locus_tag="OO585_000201"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6627913.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(31580..32701)
                     /gene="hypD"
                     /locus_tag="OO585_000202"
     CDS             complement(31580..32701)
                     /gene="hypD"
                     /locus_tag="OO585_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6627914.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(32701..32973)
                     /gene="hypC"
                     /locus_tag="OO585_000203"
     CDS             complement(32701..32973)
                     /gene="hypC"
                     /locus_tag="OO585_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6627915.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(32964..33836)
                     /gene="hypB"
                     /locus_tag="OO585_000204"
     CDS             complement(32964..33836)
                     /gene="hypB"
                     /locus_tag="OO585_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6627916.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(33840..34190)
                     /gene="hypA"
                     /locus_tag="OO585_000205"
     CDS             complement(33840..34190)
                     /gene="hypA"
                     /locus_tag="OO585_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6627917.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34402..34863
                     /gene="hycA"
                     /locus_tag="OO585_000206"
     CDS             34402..34863
                     /gene="hycA"
                     /locus_tag="OO585_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6627918.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34988..35599
                     /gene="hycB"
                     /locus_tag="OO585_000207"
     CDS             34988..35599
                     /gene="hycB"
                     /locus_tag="OO585_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6627919.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            35596..37422
                     /gene="hycC"
                     /locus_tag="OO585_000208"
     CDS             35596..37422
                     /gene="hycC"
                     /locus_tag="OO585_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6627920.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILILSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            37425..38348
                     /gene="hycD"
                     /locus_tag="OO585_000209"
     CDS             37425..38348
                     /gene="hycD"
                     /locus_tag="OO585_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6627921.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITSRITWAGFGFAFLAFVSLLAA"
     gene            38366..40075
                     /gene="hycE"
                     /locus_tag="OO585_000210"
     CDS             38366..40075
                     /gene="hycE"
                     /locus_tag="OO585_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6627922.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKEQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            40085..40627
                     /gene="hycF"
                     /locus_tag="OO585_000211"
     CDS             40085..40627
                     /gene="hycF"
                     /locus_tag="OO585_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6627923.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            40627..41394
                     /gene="hycG"
                     /locus_tag="OO585_000212"
     CDS             40627..41394
                     /gene="hycG"
                     /locus_tag="OO585_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6627924.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            41391..41801
                     /gene="hycH"
                     /locus_tag="OO585_000213"
     CDS             41391..41801
                     /gene="hycH"
                     /locus_tag="OO585_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6627925.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            41794..42264
                     /gene="hycI"
                     /locus_tag="OO585_000214"
     CDS             41794..42264
                     /gene="hycI"
                     /locus_tag="OO585_000214"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6627926.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(42423..43847)
                     /gene="ascB"
                     /locus_tag="OO585_000215"
     CDS             complement(42423..43847)
                     /gene="ascB"
                     /locus_tag="OO585_000215"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6627927.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(43856..45313)
                     /gene="ascF"
                     /locus_tag="OO585_000216"
     CDS             complement(43856..45313)
                     /gene="ascF"
                     /locus_tag="OO585_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6627928.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            45573..46583
                     /gene="ascG"
                     /locus_tag="OO585_000217"
     CDS             45573..46583
                     /gene="ascG"
                     /locus_tag="OO585_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6627929.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            46732..47259
                     /gene="hydN"
                     /locus_tag="OO585_000218"
     CDS             46732..47259
                     /gene="hydN"
                     /locus_tag="OO585_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6627930.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            47412..49664
                     /gene="hypF"
                     /locus_tag="OO585_000219"
     CDS             47412..49664
                     /gene="hypF"
                     /locus_tag="OO585_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6627931.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(49792..50925)
                     /gene="norW"
                     /locus_tag="OO585_000220"
                     /pseudo
     CDS             complement(49792..50925)
                     /gene="norW"
                     /locus_tag="OO585_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(50922..52361)
                     /gene="norV"
                     /locus_tag="OO585_000221"
     CDS             complement(50922..52361)
                     /gene="norV"
                     /locus_tag="OO585_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6627932.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            52548..54062
                     /gene="norR"
                     /locus_tag="OO585_000222"
     CDS             52548..54062
                     /gene="norR"
                     /locus_tag="OO585_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6627933.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(54059..55024)
                     /gene="gutQ"
                     /locus_tag="OO585_000223"
     CDS             complement(54059..55024)
                     /gene="gutQ"
                     /locus_tag="OO585_000223"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6627934.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(55017..55790)
                     /gene="srlR"
                     /locus_tag="OO585_000224"
                     /gene_synonym="gutR"
     CDS             complement(55017..55790)
                     /gene="srlR"
                     /locus_tag="OO585_000224"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6627935.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(55857..56215)
                     /gene="gutM"
                     /locus_tag="OO585_000225"
                     /pseudo
     CDS             complement(55857..56215)
                     /gene="gutM"
                     /locus_tag="OO585_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            complement(56320..57099)
                     /gene="srlD"
                     /locus_tag="OO585_000226"
     CDS             complement(56320..57099)
                     /gene="srlD"
                     /locus_tag="OO585_000226"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6627936.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(57103..57474)
                     /gene="srlB"
                     /locus_tag="OO585_000227"
                     /gene_synonym="gutB"
     CDS             complement(57103..57474)
                     /gene="srlB"
                     /locus_tag="OO585_000227"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6627937.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(57485..58444)
                     /gene="srlE"
                     /locus_tag="OO585_000228"
                     /gene_synonym="gutE"
     CDS             complement(57485..58444)
                     /gene="srlE"
                     /locus_tag="OO585_000228"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6627938.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(58441..59004)
                     /gene="srlA"
                     /locus_tag="OO585_000229"
                     /gene_synonym="gutA"
     CDS             complement(58441..59004)
                     /gene="srlA"
                     /locus_tag="OO585_000229"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6627939.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLALSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            59260..60345
                     /gene="mltB"
                     /locus_tag="OO585_000230"
     CDS             59260..60345
                     /gene="mltB"
                     /locus_tag="OO585_000230"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6627940.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            60490..60987
                     /gene="pncC"
                     /locus_tag="OO585_000231"
     CDS             60490..60987
                     /gene="pncC"
                     /locus_tag="OO585_000231"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6627941.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            61067..62128
                     /gene="recA"
                     /locus_tag="OO585_000232"
     CDS             61067..62128
                     /gene="recA"
                     /locus_tag="OO585_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6627942.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            62371..62871
                     /gene="recX"
                     /locus_tag="OO585_000233"
     CDS             62371..62871
                     /gene="recX"
                     /locus_tag="OO585_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6627943.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            62999..65629
                     /gene="alaS"
                     /locus_tag="OO585_000234"
     CDS             62999..65629
                     /gene="alaS"
                     /locus_tag="OO585_000234"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6627944.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEVAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            65864..66049
                     /gene="csrA"
                     /locus_tag="OO585_000235"
     CDS             65864..66049
                     /gene="csrA"
                     /locus_tag="OO585_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6627945.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            66365..66457
                     /locus_tag="OO585_000236"
     tRNA            66365..66457
                     /locus_tag="OO585_000236"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66399..66401,aa:Ser,seq:gct)
     gene            66461..66537
                     /locus_tag="OO585_000237"
     tRNA            66461..66537
                     /locus_tag="OO585_000237"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:66495..66497,aa:Arg,seq:acg)
BASE COUNT        15625 a        17067 c        18257 g        15775 t
ORIGIN      
        1 ctcctgggaa aagatgatta cccctggctg acgcatgtca cgaaaaccga tgtacattcg
       61 caccgggtag cgacgcgcaa agaagttgaa cgtagcgtga gcgtgggttg gctccatagt
      121 gaacaagagt gtattgcgcg tatcgaatcg gcggtaagtc aggggaaatg catcgcctgg
      181 attcggaatt ctgttgatga cgctattcag gtgtatcgtc agctgcttgc ccgcggcgtc
      241 attcctgctt ccagcctttc actttttcat agccgctttg cttttagcga tcgccagcga
      301 attgaaacgg aaacgctggc acgctttggt aaatattgta gtttacagag agcgtctcaa
      361 gtcattgttt gtacacaagt aattgaacaa agcgtggata ttgacctgga cgaaatgatc
      421 tccgacctgg cccccattga tttgctaatc cagcgagcgg ggcgattaca gcggcatatc
      481 cgcgatatta atggtcagtt aaagcgtgac ggaaaagacg agcgttcccc ccctgaattg
      541 ctgattctgg cccccgtctg ggatgacgct cctggtgacg aatggttcgg cagtgccatg
      601 cgtaacagtg catatgtcta tcccgatcat gggcgaatct ggctgacgca gcgtgtgctg
      661 cgtgagcaag gcgctattca gatgccgcac gcagcccggc ttcttattga atcagtttac
      721 ggtgaggacg tggtaatgcc ggaaggattt gcccgcagcg agcaggagca agtgggcaaa
      781 tattactgtg atcgcgcaag ggctaaaaag tatgtgctga acttcaggct cggctatgcc
      841 gccaatatca acgattacct tccggaaaaa ctgtcgacac gtctggctga agagtctgtt
      901 tccctgtggc tggctacctg tattgacggt gtggtgaagc cttatgccac cggtgctcac
      961 gcatgggaaa tgagcgttgt cagagtgcgc cgaagctggt ggaaaaaaca tcgggatgag
     1021 ttttctttac tggaagggga tgcgttcagg cagtggtgcg ttgaacagcg gcaagatccg
     1081 gaaatggcaa acgtgatttt agtcaccgat gacgaaagtt gcgggtattc ggccagggag
     1141 ggattgattg gcaaggttgg ttgagatgtc tgcgacaaga ctctctgaat agttgatata
     1201 cagaaaagga tggaaggatg gacaaccgac ctgggatggt cttttttacg agaggatgcg
     1261 tatgaactcg ttttcacttc tgacaacccc gtggttgccc gttcgtttta aagacggaac
     1321 gacaggcaaa ctggcaccag tcgatctggc tgatgaaaat gttgtcgata tcgctgcgcc
     1381 gcgggcagat ctccaggggg cggcctggca gtttttgctg gggctactac aaaccagctt
     1441 cgcgccaaaa gatcaacgta gttgggatga tatctgggaa gacggactgg aagctgaaaa
     1501 gctaagggaa gcattgctgt ctttagaaca tgcttttcag tttgggccag attccccttc
     1561 atttatgcag gattttgatg aattaaaagt taaggcaact tttattgctt cgcttcttcc
     1621 tgacgcacca ggaaaacaaa caaaagaacg aaatacagac cattttatta agcgagacac
     1681 aactcagcat ctgtgcctgc attgtgtgcc tttagcgctt ttttcaatcc agttgaatgc
     1741 acctataggt ggacgcggtt actatcctgg gctacgtggc ggagggcctc tgacgacact
     1801 tatagaattg ctggagtatc agggcaatca acaaacccct ttgtggcgaa agctgtggct
     1861 aaacgtaatg ccgcaagatg aagccgattt acctttacca aaaacctttg atgacctggt
     1921 ctttccttgg cttgcaccga cgcgtaccag cgaactggac ggtgctgtgg taacggatga
     1981 acaagtcaat aaacttcagg cctactgggg aatgccacga cgtatacgta ttgattttaa
     2041 gacaacctca ataggtaact gcgacatttg cggtaggcag agcgatgctc ttttgggctt
     2101 gatgtcactc aaaaactatg gtgttcaata cgtcatgtgg cgccatcctc taacacctta
     2161 ccgtctgccg cttaaagagg gtggagattt ttactcagtt aaaccacaac ctggtggttt
     2221 aatctggcgc gactggttag gcctcataga ggtaggtaac tcaaaaaaca atactgaact
     2281 ccccgcacag gtcgtgaaac ttttgaatgc cagcaatcta aagcagacaa gggtgtgctt
     2341 atggggattt ggttttgatt ttgaagatat gaaggttcgt tgttggtatg aacaccattt
     2401 cccgctgttg ttaaacaaaa aagaagacct aatacctaag ttgcgtctgg cagcgcaagc
     2461 ggcatcacac attctaagct tgttacaccg agcgttgaaa gaagcatggt tctctgagaa
     2521 gaaaaccaca aagcttgatt tcggttttgt ggatattgac ttctggaaca aaactcagca
     2581 tcgattcctg agattagtgc gcaaaattga agaaggtcag gatccggacg aattactcag
     2641 taaatggcaa aaggaaatgt ggttattcgc gcgtcaggat tttgacgatc gtgtctttac
     2701 caatccatat gagcccgttg atttggaacg cgtcatgacc gcacgcaaga aatattttac
     2761 aacatcagcg gagaagcaaa atgctaacgc cgccagggag aaaaagcagg aggctgctga
     2821 atgagtattg taaaagatga gcataaagtt gcgctgcgta aatggcatga agagttgcag
     2881 gataaacgcg gcgagcgtgc cagtctgcgg cgtagtacga cggttaatga tgtttgtctg
     2941 acggatggat ttcgcctgtt tcttaagaac agacaaataa agtggcagga tgaacccgag
     3001 tggcgtatca ccgcactagc gctgatagca gcagttagcg ccaatgttaa agctattgac
     3061 gaacggcagc cttttgccgc gcaactggcg gcggtaatga aaaaggggcg gtttactcgt
     3121 ttgttggcag taaaaacgcc ggatgattta ttgcggcagt tacgccgtgc ggtcaaatta
     3181 ttaaacggtt ccgtcaatct tgactctctg gaagaggggg tcttccgctg gtgccaggag
     3241 agcgatgacc tgctcaatca tcaccgccgc cagcagcgac caacagaatt tatccgtatc
     3301 cgctgggcgc tgggcgctgg gcgctggaat attaccaggc tggcgacgcc gataacgaac
     3361 aaaattaata aaggactatc tcatgacaac atttattcag cttcatttgt taaccgctta
     3421 ccctgctgcc aatcttaacc gtgatgattc cggctctccg aaaactgtga ttctgggtgg
     3481 agcaacgcgt ctgcgcgttt cctcgcaaag tctgaagcgt gcttggcgca cttctgaact
     3541 ttttgaacag gcgctggcgg gccatattgg tgttcgtagc gggcgtattg cgcgtgaggc
     3601 ggcaactatc ctgattgaga aaggaatcga agataaaaaa gccatcgaat gggcagtaga
     3661 gatagccgat tatcttggga aagccaaaaa agacaaaaaa caaaaaaacg acaaaaaacc
     3721 aaaagatccg ctcactagcg ccgaaactga acagctcgtc catatcagcc cggcagaatt
     3781 tgacgccgta aaagcgctgg cccatcaact ggcagaagaa aagcgcgcgc caaaagagaa
     3841 agatctcgct ttgttacgta aagaccgtat ggcggtagat atcgctatgt ttggtcgtat
     3901 gctggcgaaa aaacccgggt ttaatgtaga agccgcctgc caggtcgcgc atgcgtttgg
     3961 tgtcagtgaa acgattgtcg aaaatgattt tttcaccgcc gtagatgatt tacgccaggc
     4021 ttctgaagat gccggtgccg gtcatgtaga tgaaaccgga ttcggttctg cgctgttcta
     4081 cacctatatc ttatctgcat tgataaagat ctgctggtag aaaacctcgg cggtgacgaa
     4141 gcgttagcta atcagaccat acgcgccttt acggaagccg cgcttaaagt ctccccaacc
     4201 ggtaaacaaa acagctttgc cagccgggct tatgcctcct gggcgctggc agaaaaaggc
     4261 accgatcaac cgcgttctct ggcggcagct ttctatgaac ccattaatgg cacccgtcag
     4321 ttagaggtgg cggtgcagcg tattacaact cttcgcgaaa atatgaatac ggtctacgaa
     4381 cagaagaccg attacgcaag ttttgacgtg atgaacaaac agggaagcat gaaggacgtg
     4441 ctggacttta tctgcgcgta aggggcggct tatgagccaa tatctgattt ttcagcttca
     4501 tgggccgatg gcatcctggg gcgtcgatgc ccccggcgaa gtgcgtcata cccatgaact
     4561 gccttcgctc tcagcattgc tggggctgct ggctgccggg gtagggatcc ggcgtgatga
     4621 taccgaacgc ttaaacgcgt ttaatcgcca ctattcactg gtggtttgcg ccagccgtaa
     4681 cccgcgctgg gcacgggatt atcacacggt ccagatgcca aaagaggtgc gtaaagcacg
     4741 ttatttcagc cgtcgcgaag agttgagcga ccctgagctt ttgagcgcga ttatctcccg
     4801 gcgcgactac tacaccgatg cctggtggat ggtggcggtg gcaacaacag ccgatgcgcc
     4861 ttacagcctc gaacagttgc aggacggttt acgccatcca gtttttccgc tttatctggg
     4921 gcgaaaaagt catccgctgg cattacctct tgcgccgtta ctgctcgaag gcaacgcgtg
     4981 tgatgcctta tgtaatgcat accaacagta tcaggatcat ttccataaac tgaaagtttc
     5041 actcccgaaa cttcaggatg aatgctggtg ggaagggaaa cacgacggcc tggtcgcgag
     5101 caaaatatta cgccgccggg atgttccttt aaatcgtcag cagtggctgt ttggggaacg
     5161 caccgtcaat caggggccgt ggctcagcaa ggaggaacca tgtacctctc aagaataacc
     5221 ttacataccg gccagctttc gcctgcgcag ttgctgcatc tggtggatcg cggtgaatat
     5281 gtgatgcatc agtggctgtg ggatctcttc cctagcggca aagaaaggca atttctttat
     5341 cgtcgggaag aactccaggg cgcgtttcgc ttttttgtac tgtcgcagga acgcccggcg
     5401 gaaagtgata cgttcaccat agaatgccgg tcatttgcgc ccgagctgtg taccggacaa
     5461 caactttgtt tcaacctgcg ggcgaatcca acaatctgta aagcgggcaa gcgccacgat
     5521 ctgctgatgg aggcgaaacg gcaggtgagg gggcaggcgg aaggaagtga tgtctggttg
     5581 catcaacaac aggcggcgct ggactggctg gcagcgcagg gggagagaag tggttttaca
     5641 ctgctggata tctcggttga tgcctaccga caacagcaac tgcggcggga aaactcccgg
     5701 caactgatcc agttcagtag cgtggattat acggggatgc ttacggtcac cgatcccggg
     5761 ttatttctgc aacgtctcag tcaggggtat ggcaaaagcc gcgcgttcgg ctgtggtctg
     5821 atgctgatca aacccggagc agaggcgtga cgtttgtacc gctgagcccg atcccgttaa
     5881 aagatcgcac ctctatgatc ttcctccagt acgggcaaat cgacgtactg gacggcgctt
     5941 tcgtgctgat cgataaaacc gggatccgca cgcacattcc ggtgggatcg gtcgcctgca
     6001 ttatgctcga accgggaacg agagtttccc acgcggcggt gcatctggcc gccacggtgg
     6061 ggacgctaca ggtctgggtc ggtgaagcgg gtgttcgcgt ctactcttcc ggacaacccg
     6121 gtggggcgcg tgctgataag ttactctacc aggcaaagct ggctttaacg gaagatctac
     6181 gtctgaaggt ggtgcgcaaa atgtatgaat tacgttttcg tgagccaccg ccagctcgcc
     6241 gttcagtgga gcagctacgt ggaattgagg gatctcgcgt tcgccagacg tatgcattac
     6301 tggcgaaaca atatggtgtg aaatggaatg gtcgcaaata cgatcctaaa gactgggaaa
     6361 aaggcgatgt tgtgaatcgc tgcatcagtg ctgctacatc atgtctgtac ggtatatctg
     6421 aagcggcagt attagccgcg ggatatgcgc ccgctattgg gtttatccat agtggcaaac
     6481 cgctttcatt tgtttatgac atagccgata tcattaaatt tgattcggtt gtaccaaagg
     6541 catttgaaat agcggcgagg caacccgcag aacctgataa agaagtcaga ttagcctgtc
     6601 gtgatatttt ccgtagcact aagttaacgg gcaaattaat accgttaatt gaggaagtcc
     6661 ttgccgcagg tgaaattgaa ccgccacaac ctgcgccgga tatgttaccg ccagccatcc
     6721 ccgaacctga aacgttgggc gatagcggtc atcgaggacg cggctgatga gcatggtcgt
     6781 ggttgttaca gaaaatgtcc cgccacgctt acgtggacgg ctcgcaatct ggctactgga
     6841 agtgcgtgcc ggtgtgtatg ttggtgatac atcaaaacgt attcgggaga tgatctggca
     6901 gcaaattacc caactggctg gttgcggaaa tgtggtgatg gcctgggcga ccaataccga
     6961 gtcgggtttt gaatttcaga cctggggaga aaacagacgt attccggtgg atttggatgg
     7021 gttacgtttg gtttcttttc ttcctgttga taatcaataa gttatccgtt ctttaaaaat
     7081 aaggaaatgt tttaatttag ttggtagatt gttgatgcgg aataaatttg tttaaaaaca
     7141 gttatgtatg cttagtgtgt tccccgcgcc agcggggata aaccgggcgc actggatgcg
     7201 ataatggata tcacttagaa ttccccgccc ctgcggtaga actcccagct cccattttca
     7261 aacccatcaa gacgccttcg ccagctcctt caccaacggt agcattatcc gcataacgtc
     7321 acggcagcga cgttctattc ttccaggaag tgccttatca atatgctgtt gattatccag
     7381 tcttacgtca tgccagctat ttcccgcagg gaatgcggct gtttttgcgc gttgctgata
     7441 accatcctta ttcccaagat tccagttagt cgcttccacc gaaagtacag caatgcccgc
     7501 tttgtcgaat atttctgcgt cattacaaca cccagtgcct ttcggataat ttttattcaa
     7561 ccccggattg gtcgttgcgg ctattccgtg actgcgcgca attgccagcg ccctgtcgcg
     7621 cgttaatttc cttactgctt cagtggtttt tacaccgctg ttgaaataca atttatcgcc
     7681 aacaattaag ttatcgagat taatcaccag cagcgtattt ttcttttcgg tgtcactcat
     7741 ccgcttgagt aaattctcag cgcctaattt cccttcctct tcgccgctgg tcgccacaaa
     7801 tcgaatacca tactcggtag gcgtattttt caggcgttct gccaattcca gcatgacacc
     7861 taaacctgcg gcgttatcat ccattccttg taacgtcagc ccgccgagat tggcatcggc
     7921 gtcagcatcg ctcagcgggg cgtaagtatc cagatgcgcc ataatgatga tctgctgcgg
     7981 cgcttttcct tcatgagcgg caatcaccgt acttcccgtc acgttatgcc agctcttacg
     8041 attatcgcgg gcggtataaa tataccgact attaaatgtc cgaatatcac tgcgataacc
     8101 catttgctga aactgttggc gaatataatc ggcagataac atttctgcag gagttccggt
     8161 catgcgtccc ggaaagaaag tagcaatatg tcgtgcctga gtattagcaa aatcgccagg
     8221 tttaggtgac gaggcgtgta cggggagaat aaagcatacg ccgagcgcca gggcagcggt
     8281 acggtggcgc aatgcggaaa acatagtgag tccttaaata ccatgcaaat ttttttaccg
     8341 ccatagtatg aaactgccgc tgcgctaaaa caatttcaaa tcttcctaaa cgcccgaaat
     8401 ccggtgcctt aagcactttt tgatattagc tttgccaaat cgttattccg ttaaggaact
     8461 actcattcta attggtaatt tcattcgttc tcttacgctc cctatagtcg aaacatctga
     8521 tggcaagaaa atagcggtat tgcaaaggaa cggttatggc tcaaaaacga cttactcacc
     8581 tgcggcaact ggaggcggaa agcatccaca ttattcgcga ggtggcggca gaattctcaa
     8641 atccggtgat gctctactct atcggtaaag attccagcgt catgctgcat ctggcgcgca
     8701 aggcgtttta tccaggtacg ctgcctttcc cgttgctgca tgtcgatacc ggctggaaat
     8761 tccgcgagat gtatgagttc cgcgatcgta ctgctaaagc ctacggctgc gagctgctgg
     8821 tgcataaaaa cccggaaggc gtggcgatgg ggattaatcc attcgtacac ggcagcgcga
     8881 aacataccga tattatgaaa actgaaggcc tgaaacaggc gctgaacaaa tacggttttg
     8941 atgccgcctt cggtggtgcg cgccgtgatg aagagaaatc ccgcgctaaa gagcgaatct
     9001 actctttccg tgaccgcttc catcgctggg atccgaaaaa tcagcgcccg gagctgtggc
     9061 acaactacaa cgggcaaatt aacaaaggtg aaagcatccg cgtcttcccg ctctctaact
     9121 ggaccgagca ggatatctgg caatacatct ggctggaaaa tatcgacatt gtgccactgt
     9181 atctcgctgc ggaacgtccg gttctggaac gcgacggtat gttgatgatg attgatgaca
     9241 accgtatcga cctgcaaccg ggcgaagtga ttaaaaaacg gatggtgcgt ttccgtacgc
     9301 tgggctgctg gccgctgacc ggtgcggtgg agtcaaatgc acaaacactg ccggagatca
     9361 tcgaagagat gctggtttct accaccagtg aacgtcaggg ccgcgtgatt gaccgcgacc
     9421 aggcggggtc tatggagctg aaaaaacgtc aggggtattt ttaagatgaa caccgcactt
     9481 gcacaacaaa tcgccaatga aggcggcgtc gaagcctgga tgattgcgca acaacataaa
     9541 agcctgctgc gttttctgac ctgtggtagc gtcgatgacg gcaaaagtac cctgattggt
     9601 cgcctgctgc acgatacccg ccaaatctac gaagatcagc tctcatcgct gcataacgac
     9661 agtaagcgtc acggcaccca gggcgaaaag ctggatctgg cactgctggt ggacggcctg
     9721 caagctgagc gcgaacaggg catcaccatt gacgtggctt accgttattt ctctaccgag
     9781 aagcgtaaat ttattatcgc cgacacccca gggcacgagc agtacactcg caatatggca
     9841 actggcgcat cgacatgtga actggcgatc ttactgatcg atgcccgtaa aggcgtgctc
     9901 gatcaaaccc gtcgtcacag ttttatctcc acactgttgg ggatcaaaca tctggtcgtg
     9961 gcgatcaaca aaatggatct ggtggattac agtgaagaga cgttcaccca tattcgtgaa
    10021 gattatttga cctttgccgg gcagctgccg ggtaatctgg atatccgctt tgtgccgctc
    10081 tctgcactgg aaggcgacaa cgtggcatcg caaagtgaaa gtatgtcgtg gtacagcggt
    10141 ccgacactgc tcgaagtgct ggaaaccgtg gagatccagc gagtggtgga tgcccagcca
    10201 atgcgcttcc cggtgcagta cgttaatcgc ccgaatctcg attttcgtgg ctacgccgga
    10261 acgctggcat ccggtcgcgt ggaagtcggg caacgtgtca aagcgctgcc ctctggtgtg
    10321 gaatcaaacg tcgcgcggat cgtgactttt gatggtgatc gcgaagaagc ctttgccgga
    10381 gaagcgatca ccctggtgct gacggatgag atcgacatca gccgtggcga tctgctgctg
    10441 gcggcagacg aagcgttacc ggcggtgccg agcgcgtcgg tggatgtggt atggatggcg
    10501 gaacagccgc tttccccggg ccagagttac gacatcaaaa ttgccggtaa gaagacgcgc
    10561 gcgcgtgttg atggcattcg ctatccggtt gatattaata accttaccca gcgtgaagtt
    10621 gaaaacctgc cactgaatgg gatcggcctc gtggatctca cttttgacga gccgctggtg
    10681 ttagatcgtt atcaacaaaa tccggtgacg ggtgggctga tttttatcga tcgcctgagc
    10741 aacgtgactg ttggtgccgg tatggtgcat gagccgaaaa acgaggccgc cgttgcgtca
    10801 tctgaattca gtgcattcga actggaattg aatgccctgg ttcgtcgcca ctttccgcac
    10861 tggggcgcgc gcgatttgct gggggataaa taatggcgct gcatgacgaa aacgtcgtct
    10921 ggcatagcca tccggtcact gtgcaacaac gcgagctaca ccacggtcat cgtggtgtag
    10981 tgctgtggtt taccggcctc tctgggtccg gtaaatcaac ggtcgccggg gcgctggagg
    11041 aggcgttaca taaactcggc gtcagtacgt atctgctgga tggcgacaat gttcgccacg
    11101 gattatgcag cgatctcggt tttagcgatg ccgatcgtaa agagaatatc cgtcgcgtcg
    11161 gtgaagtggc gaatttgatg gttgaagccg gactggtggt gctgaccgca tttatctcgc
    11221 cacaccgcgc cgaacgccag atggttcgcg aacgcgtagg agaagggcgc tttatcgaag
    11281 tgtttgtcga tacgccgctg gcgatttgcg aagcccgcga tcccaaaggc ttatataaga
    11341 aagcgcgtgc cggtgaactg cgcaacttta cgggaataga ttccgtttac gaagcgcctg
    11401 aatcggcaga aattcatctc aatggtgaac aattagtaac aaatttggta cagcaattat
    11461 tagatctgtt gagacagaac gatattatca gatcctgaga caccaccggg tttccatgcc
    11521 cggttcgtca aagtaacagg atcaaatatg cgtaatagcc ataacattac actaacaaat
    11581 aacgacagcc ttactgagga tgaagaaacc acatggtcac tgcctggtgc cgtggtcggt
    11641 tttatctcct ggttatttgc gctggcgatg ccgatgttga tttatggctc taacacgctg
    11701 ttcttcttta tctacacctg gcctttcttt ctggcactga tgcctgtcgc ggtagtggtg
    11761 gggattgcgc tgcattcatt gatggacgga aagctacgct acagtattgt tttcaccctg
    11821 gtgacagtag gcattatgtt tggcgcactg tttatgtggc tactgggcta atttgtactt
    11881 tcttgtctcc tctgttcata attcaaaccg taactaataa tgagattatg ttctgcacgc
    11941 cctaggtata cgtaacaatg gacaaatgtg gtacatttgc ccgcgttgtc gcggtatccc
    12001 caacagagga tgtagagtcg tcttcggatg catgggatga tgatgccgtt tttcaggggg
    12061 caggatgggt aaactaacgc tgctgttgct ggctattctg gtctggctac agtattcgct
    12121 gtggttcggt aagaacggta tacatgacta tacccgcgtc aatgatgatg tggcggcaca
    12181 gcaagctaca aacgcgaaac ttaaagcgcg aaacgatcaa ctttttgccg aaattgacga
    12241 tctcaatggc ggccaggagg cgctcgaaga gcgtgcgcgt aatgaactca gcatgaccag
    12301 gccgggcgaa actttttatc gtctggtgcc tgacgcgtcg aagcgcgcac agtctgcggg
    12361 gcaaaacaat cgataaatca gcccgggaat taacatggca accactcatt tggatgtttg
    12421 cgccgtggtt ccggcggccg gatttggccg tcgaatgcaa acggaatgtc ctaagcaata
    12481 tctctcaatc ggtaatcaaa ccattcttga acactcggtg catgcgctgc tggcgcatcc
    12541 ccgggtgaaa cgtgtcgtca ttgccataag tcctggcgat agccgttttg cacaacttcc
    12601 tctggcgaat catccgcaaa tcaccgttgt agatggcggt gatgagcgtg ccgattccgt
    12661 gctggcaggt ttgaaagccg ctggcgacgc gcagtgggta ttggtgcatg acgccgctcg
    12721 tccttgtctg catcaggatg acctcgcgcg attgttggcg ttgagcgaaa ccagccgcac
    12781 gggagggatc ctagccgcac cagtgcgcga tacgatgaaa cgtgccgaac cgggcaaaaa
    12841 tgccattgct cataccgttg atcgcaacgg cttatggcac gcgctgacgc cgcaattttt
    12901 ccctcgtgag ctgttacatg actgtctgac gcgcgctcta aatgaaggcg cgactattac
    12961 cgacgaagcc tcggcgctgg aatattgcgg attccatcct cagttggtcg aaggccgtgc
    13021 ggataacatt aaagtcacgc gcccggaaga tttggcactg gccgagtttt acctcacccg
    13081 aaccatccat caggagaata cataatgcga attggacacg gttttgacgt acatgccttt
    13141 ggcggtgaag gcccaattat cattggtggc gtacgcattc cttacgaaaa aggattgctg
    13201 gcgcattctg atggcgacgt ggcgctccat gcgttgaccg atgcattgct tggcgcggcg
    13261 gcgctggggg atatcggcaa gctgttcccg gataccgatc cggcatttaa aggtgccgac
    13321 agccgcgagc tgctacgcga agcctggcgt cgtattcagg cgaagggtta taccctgggc
    13381 aacgtcgatg tcactatcat cgctcaggca ccgaagatgt tgccgcacat tccacaaatg
    13441 cgcgtgttta ttgccgaaga tctcggctgc catatggatg atgttaacgt gaaagccact
    13501 actacggaaa aacttggatt taccggacgt ggggaaggga ttgcctgtga agcggtggcg
    13561 ctactcatta aggcaacaaa atgattgagt ttgataatct cacttacctc cacggtaaac
    13621 cgcaaggcac cgggctgctg aaagccaatc cggaagactt tgtggtggtg gaagatttgg
    13681 gctttgagcc tgatggtgaa ggtgagcata ttctggttag aatcctcaaa aacggctgca
    13741 atacccgttt tgtggcggat gcactggcga aattcctgaa aattcatgcc cgtgaagtca
    13801 gcttcgctgg gcaaaaagac aaacatgctg ttacggaaca gtggttatgc gctcgcgtgc
    13861 caggcaagga aatgcccgat ctgagcgcct ttcaactgga aggctgccag gtgctggagt
    13921 atgcgcggca caagcgcaag ctgcgtttag gcgcgctgaa aggtaacgcc tttaccctgg
    13981 ttctacgcga agtgagcaat cgcgatgacg ttgaacaacg tctgatcgat atttgcgtaa
    14041 aaggtgtacc gaactacttc ggtgcccaac gttttgggat tggcggtagc aacttgcagg
    14101 gcgcgctgcg ctgggcgcaa accaatactc cggtgcgcga tcgcaataaa cggagttttt
    14161 ggttgtcggc agcccgcagt gcgttgttta atcagattgt tgctgagcgc ctcaaaaaag
    14221 cagacgttaa tcaagttgtt gacggcgatg cgctacaatt agccggacgt ggtagctggt
    14281 ttgtagcaac caccgaagaa ctggcggaat tacagcatcg cgtcaacgat aaagagctga
    14341 tgataaccgc cgcattgccg ggcagtggcg aatggggaac tcagcgtaaa gcgctggcat
    14401 tcgaacaagc agctgtcgcc gcagaaactg aattacaagc tttactggtg cgcgaaaaag
    14461 ttgaagccgc gcgcagagcg atgctgctgt atccgcaaca attaagctgg aattggtggg
    14521 atgacgtcac cgtagagatc cgtttctggc ttccggcggg tagttttgca accagcgttg
    14581 tcagggaact tatcaacaca acaggtgatt atgcgcatat tgctgagtaa tgatgacggg
    14641 gtacatgcac ccggtataca aacgctggcg aaagccttgc gtgagtttgc tgacgttcag
    14701 gtggtcgccc ccgatcgtaa ccgcagcggc gcttcaaatt ctctgacact ggaatcctcc
    14761 ctgcgcacgt ttacctttga aaatggtgat attgctgtgc aaatgggaac cccgaccgat
    14821 tgcgtctatc ttggcgtgaa tgctctgatg cgtccgcgcc cggacattgt tgtgtccggc
    14881 attaacgccg ggccgaatct gggggatgac gttatttatt ccggtacggt agccgccgcg
    14941 atggaaggcc gtcatttagg ttttccggcg ctggccgtct cgcttgacgg gcataaacat
    15001 tacgacactg ccgcggcggt aacctgttca attttgcgcg cactgtgtaa agagccgctg
    15061 cgcaccggac gtattcttaa tattaacgtt ccggatttac ccttggatca aatcaaaggt
    15121 attcgcgtga cgcgctgcgg tacacgacat ccggcagatc aggtgatccc gcagcaagat
    15181 ccgcgcggca atacgctgta ctggattggc ccgccgggcg gtaaatgtga tgccggtccg
    15241 gggaccgatt ttgctgcggt agatgagggc tatgtctcca tcacgccgct gcatgtggat
    15301 ttaactgcgc atagcgcgca agatgtggtt tcagactggt taaacagcgt gggagttggc
    15361 acgcaatggt aagcagacgc gtacaagcac ttctggatca attacgtgcg caaggtattc
    15421 aggatgagca ggtgctgaat gcacttgctg ccgtgccgcg tgaaaaattc gttgatgaag
    15481 cgtttgaaca aaaagcctgg gacaatatcg ccttgccgat aggtcagggg cagacaattt
    15541 cgcagccgta tatggtggcg cgaatgaccg aattactcga gctgacgccg cagtcgcggg
    15601 tgctggaaat tggcaccggt tcgggatatc aaacggcaat cctggcgcat cttgtccagc
    15661 atgtttgctc ggtcgaacgg attaaaggtt tgcagtggca ggcgcgtcgc cgcctgaaaa
    15721 atcttgattt acataatgtt tcaacccgtc atggcgatgg atggcaaggt tggcaggcac
    15781 gcgcgccgtt tgacgctatc attgttacgg cggcaccgcc ggaaattcca actgcgctaa
    15841 tgacgcagct ggacgaaggc gggattctcg tcttacccgt aggggaggag caccagtatt
    15901 tgaaacgggt gcgtcgtcgg ggaggcgaat ttattatcga taccgtggag gccgtgcgct
    15961 ttgtcccttt agtgaagggg gagctggctt aaaacgtgag gaaatacctg gatttttcct
    16021 ggttattttg ccgcaggtca gcgtatcgtg aacatctttt ccagtgttca gtagggtgcc
    16081 ttgcacggta attatgtcac tggttattaa ccaatttttc ctgggggata aatgagcgcg
    16141 ggaagcccaa aattcaccgt tcgccgcatt gcggctttgt cactggtttc gctatggctg
    16201 gcaggctgtt ctgacacttc aaatccaccg gcaccggtca gctccgttaa tggcaatgcg
    16261 cctgcaaata ccaattctgg tatgttgatt acgccgccgc cgaaaatggg gacgacgtct
    16321 acagcccagc aaccgcaaat ccagccagtg cagccagtag ctcagcagcc ggtacagatg
    16381 gaaaacggac gcatcgtcta taaccgtcag tatgggaaca ttccgaaagg cagttatagc
    16441 ggcagtacct ataccgtgaa aaaaggcgac acacttttct atatcgcctg gattactggc
    16501 aacgatttcc gtgaccttgc tcagcgcaac aatattcagg caccatacgc gctgaacgtt
    16561 ggtcagacct tgcaggtggg taatgcttcc ggtacgccaa tcactggcgg aaatgccatt
    16621 acccaggccg acgcagcaga gcaaggagtt gtgatcaagc ctgcacaaaa ttccaccgtt
    16681 gctgttgcgt cgcaaccgac aattacgtat tctgaatctt cgggtgaaca gagtgctaac
    16741 aaaatgttgc cgaacaacaa gccagctgcg accacggtca cagcgcctgt aacggtacca
    16801 acagcaagca caaccgagcc aactgtcagc agtacatcaa ccagtacgcc tatctccacc
    16861 tggcgctggc cgactgaggg caaagtgatc gaaacctttg gcgcttctga ggggggcaac
    16921 aaggggattg atatcgcagg cagcaaagga caggcaatta tcgcgaccgc agatggccgc
    16981 gttgtttatg ccggtaacgc gctgcgcggc tacggtaatc tgattatcat caaacataat
    17041 gatgattacc tgagtgccta cgcccataac gacacaatgc tggtccggga acaacaagaa
    17101 gtgaaggcgg ggcaaaaaat agcaaccatg ggtagcaccg gaaccagttc aacacgcttg
    17161 cattttgaaa ttcgttacaa ggggaaatcc gtaaacccgc tgcgttattt gccgcagcga
    17221 taaatcggcg gaaccaggct tttgcttgaa tgttccgtca agggatcacg ggtaggagcc
    17281 accttatgag tcagaatacg ctgaaagttc atgatttaaa tgaagatgcg gaatttgatg
    17341 agaacggagt tgaggttttt gacgaaaagg ccttagtaga agaggaaccc agtgataacg
    17401 atttggccga agaggaactg ttatcgcagg gagccacaca gcgagtgttg gacgcgactc
    17461 agctttacct tggtgagatt ggttattcac cactgttaac ggccgaagaa gaagtttatt
    17521 ttgcgcgtcg cgcactgcgt ggagatgtcg cctctcgccg ccggatgatc gagagtaact
    17581 tgcgtctggt ggtaaaaatt gcccgccgtt atggcaatcg tggtctggcg ttgctggacc
    17641 ttatcgaaga gggcaacctg gggctgatcc gcgcggtaga gaagtttgac ccggaacgtg
    17701 gtttccgctt ctcaacatac gcaacctggt ggattcgcca gacgattgaa cgggcgatta
    17761 tgaaccaaac ccgtactatt cgtttgccga ttcacatcgt aaaggagctg aacgtttacc
    17821 tgcgaaccgc acgtgagttg tcccataagc tggaccatga accaagtgcg gaagagatcg
    17881 cagagcaact ggataagcca gttgatgacg tcagccgtat gcttcgtctt aacgagcgca
    17941 ttacctcggt agacaccccg ctgggtggtg attccgaaaa agcgttgctg gacatcctgg
    18001 ccgatgaaaa agagaacggt ccggaagata ccacgcaaga tgacgatatg aagcagagca
    18061 tcgtcaaatg gctgttcgag ctgaacgcca aacagcgtga agtgctggca cgtcgattcg
    18121 gtttgctggg gtacgaagcg gcaacactgg aagatgtagg tcgtgaaatt ggcctcaccc
    18181 gtgaacgtgt tcgccagatt caggttgaag gcctgcgccg tttgcgtgaa atcctgcaaa
    18241 cgcaggggct gaatatcgaa gcgctgttcc gcgagtaagt aagcatctgt cagaaaggcc
    18301 agtctcaagc gaggctggcc ttttctgtgc acaataaaag gtccgatgcc catcggacct
    18361 ttttattaag gtcaaattac cgcccatacg caccaggtaa ttaagaatcc ggtaaaaccg
    18421 agaatggtcg ttaacactgt ccaggttttc agaccgtctg ctaccgacaa ccccagatat
    18481 ttggtcacaa tccagaaccc tgagtcatta atatgtgacg cgccaagccc accaaagcag
    18541 gctgccagcg tcaccaatac gcactgaatc ggattcaatc ccatcaccgc ttctgagagt
    18601 aacccgccgg ttgtcaggat tgctacggtt gctgacccct gcgatgcacg cagagccagt
    18661 gaaataataa atgcggctgg taacagaggc aggtcaatca tttgtagcat attggcaagg
    18721 gctttgccga cgcccgattc caccagcact ttgccaaata cccctccagc accagtaacc
    18781 aaaatcacta ccgccgcagt gggaagggct gagcccataa tgtcgctggt gtgttgtaag
    18841 ctccagccgc gacgtaaagc caataaccag aatgccagca ccagcgcaat cattagagct
    18901 accattggtg agccgatcag ctgtagcgta ccaagcaggg gatgcgaagg cggcatcagt
    18961 gttgcggaaa ccgtacccgt catgataatc gcgataggaa taacaattag cgaggtgacc
    19021 agcgcgacgc ccggtggatt tattttatcg cttaattttg tcgcgccttc ctcactggcc
    19081 ggagccagtt gcatctgttc cagtacttct actgacatcg catattggcg cttattgatt
    19141 attttcgctg caaagtagcc aacaatccct acgggaatag aaatcgcaat accgatgatg
    19201 gttagccagc cgatgtctgc gtggagtaac cccgctgtgg cgacagggcc tggatgcggc
    19261 ggtactgcca catgaacagt tagcatgatt ccagcgacag gcaggccaaa tttgagtggc
    19321 gatattttgg caaccttggc aaaaccgtaa atgattggcg caagaataat aaagctgaca
    19381 tcaaagaaga cgggaatacc gaggaagaac gctgccagag tcagcgcagc gatagttcgt
    19441 ttgtcaccta acttgcgact gaaataatta gccagtgact ctgcaccacc agagtgttcg
    19501 atcatacgcc ccagcatagc gcccagacca ataataatag tgacggaacc aagcacaccg
    19561 cccatcccgg cgatcatcac tttacccact tcgcccgccg gtatacccgc cgcaagtgcg
    19621 actaacaggc tgacgaggag cagagcaacg aatggttgta cctttgcctt gatgaccagc
    19681 agcaacagca tgattacgcc agctaacgca atgcataaca atgtaattgt ggacatggga
    19741 aaccctgtct gaaagttata gttaacctac cccatccgta gatgggggga tgtatgggta
    19801 cgttgtaatt agggatttaa cgaattagcg ccaggcgtca aaccagccaa gcccttcttc
    19861 ggtgaggcca cgaggtttat attcacaacc gatccagccc tgatatccca cctcatcgaa
    19921 caggcggaac agccacggat agttgatttc tccatcgtcc ggtcatgtcg atcaggtagt
    19981 ccggcaattt gtacgtgcgc atatttccca gcgtagtcgc ggattaaatg cgtcaggttg
    20041 ccatctactt tttgcgcatg aaaagtatct agttgaataa acacgttatc tcgcgcaacc
    20101 tcttcaacaa tagccagtgc ctgatactgg ctggagaaga gataatgagg cttaacgtcg
    20161 gggctgagtg cttcaactaa tattcgcttg ccgtgtggcg caaagcggtc ggcagcgtag
    20221 cggagattat cgataaatac tgcccggtac cgttcagcat cttcgccagc gggcacgacg
    20281 cctgccatca catggacttg ttcacaattg agcgccaatg catattccag tgccaggtcg
    20341 atgtctgcgt gtgcttcgtg ctcacgtccg ggaagggcgg ataatcccca ttcccccgca
    20401 ttaatatctc cgggagcggt attgaacagc gccagtgtca gatggttttg ctccagttgc
    20461 ttttggattt gcagggtgga gtagtcatag ggaaacagaa attccacagc atcgaacccg
    20521 gcttttcgcg ctgcggcgaa gcgttcaata aaaggcactt cggtgaacat catggataaa
    20581 ttagctgcaa aacgaggcat tgcattaact ccttaattcc gcaatttcac ctgcggtcag
    20641 ataacggatc gggcggtcac cgagaataaa aatcagcttt gccgtttcct ccagctcttc
    20701 catattgttg gcggcttctt gcaggctttc accgcaaacc actgggccat gatttgccag
    20761 taaaaaagcc tgattgtctg ctgccagttc cgccagatcc tgtgcgatgc gtttatcgcc
    20821 cggtcggtaa tagggcacca gcgggacatt tcccatccgc atcaccacgt atggtgtgaa
    20881 cggacgaata acgttgctgc tgtccagccc ttgcaggcag gaaagcaccg tcgaccatgt
    20941 gctgtgcaaa tgcaccaccg ctttacagcg cggattgttg cgatacagcg ccagatgaaa
    21001 gagcacctct ttcgagggtt tgtcaccact taaccattcg ccatccgcgg cgactttgga
    21061 aagccgctgc ggatcgagat tgcccaggca tgaacctgtc ggtgtcgcca gtaaattccc
    21121 gtcaggtaaa agcagcgaca gattgccagc cgaaccggtt gcatagccgc gctgaaagaa
    21181 tgaactggca atccgcgtca tctcctctcg caaagactgc tctacttttg cgaaatcgct
    21241 catgataaaa actctctttg ggctcgtgaa aaaaaggctt catcaccgaa gttgccagat
    21301 ttaagggcga gtgagacagg cttatccagt gcgtttaccc acggcacgcc gggggaaatg
    21361 gttgggccaa tatgaaaccc ttttatgccc aggctctgtg tgactacgcc ggaggtctca
    21421 ccgcctgcga caataaagcg tgtcacgcct tccgctgcta accgcgccgc tagttgagaa
    21481 aacagagttt ctactgcctg actggctttt tgtgcaccgt attgctgttg aattgctgcc
    21541 aatgcgtcag tgctggcggt ggcaaaaacc agtggagcaa gtacactttc ctggcccaga
    21601 acccactctg ccagttcgtg tgcataagcg gccagagttt cggttgagag gcagcgtgcc
    21661 acatcaactt cacgggctgg tgcaatttga cggtaatgtg ccacctggcg gttggtcatt
    21721 tgagagcatg aaccggagag cactacgccg cgcccagcga gcggatgccc tgcttcgcga
    21781 gcctggttac cgttttcttg cgcccactgc cgggccaggc caatcgccag accagaaccg
    21841 cccgttacca gtggggcatc gcgcaaggct tctccctgaa tttccagatg gtgttcggtc
    21901 agcgcatcaa gcaccgcgta gcggtagccc tcttgctgta agcgagccag ctcttgacga
    21961 acggcatcca caccttgttc gaaaacatgt gccgaaacga cgccgcagcg ccctgtggat
    22021 tgcgcttcaa ccagacgggg aagatagctg tcggtcatgg gatttaccgg gtgatggcgc
    22081 atcccggatt cggccagcag ttgattcatt acgaacaaat acccctgata aaccgtacgt
    22141 ccgttgaccg acagggccgg agagaagacc gtaaacggcg tgtcgagagc atccattaat
    22201 gcatcggtaa ccgggccgat attacctttc gccgtactgt cgaaagtaga gcagtatttg
    22261 aaatagatct gtttgcaacc ttgctgttgc aaccagctca gagccgccag cgattgctgt
    22321 gtggcttcaa ccaccggaca ggagcgcgtt ttcaggctga tcaccagtgc gtcgattgct
    22381 tccggcattt tacctgttgg aacaccgtta atttgtaccg ttggtagacc gttttccacc
    22441 agaaaactgg cgatatccgt cgcgccggta aaatcatcgg cgataacgcc aatcttgatc
    22501 atgatttcgc tcccggtaga gtgatgccag agaaaatctt gataactgcg ctatcgtctt
    22561 ctttcccgta acctgcgtta ctggcgctgg tgaacatatt caatgctgtt gaggccaatg
    22621 gcagcgggaa gtgcagggct ttggctgtat cggcaaccag accaagatcc ttaacaaaaa
    22681 tatcgacggc tgaatgcggg gtgtaatcgc catccaccac atgacgcatc cggttttcga
    22741 acatccagga atttccggcg gcattggtca cgacgtcata catcacatcc agcgggatcc
    22801 ccgcacgggc tgcaagtgcc atcgcttcgg ctccggcagc aatatgtacg cccgctaaca
    22861 actggtgaat aatttttacg gtcgaaccta gtcccggttc tgcacctatg cgataaactt
    22921 ttccggcaac ggcttccagc acgggtgcca gtcgttcaaa ggcaatatcg ctaccggagg
    22981 ccatgacagt catttcaccg ttagcggctt ttactgcatc accagaaact ggcgcatcca
    23041 gcatttccag atcgaatcca gccagagcgg tagcaatttc ttgcgcatca gcactagcga
    23101 tagtggaaga aaccattact gccgtaccgg gtttcagatg ttgtgcaacg cctgtttcac
    23161 caaacagcac ctgtttaacc tgggccgcat tgaccaccag caccagcaga gcgtccagtt
    23221 tttcggcaaa cgtcgcggcg ttatcagaaa ccccgcaagc acctgcctct ttcaacgtag
    23281 cgcaggcatt gctgttcagg tctgcgcccc aggtagaaag acctgcgcgg acacatgaca
    23341 gtgctgctcc cattcccatt gaccctaagc caacgatacc gacatgaaac tcagatcccg
    23401 ttttcatctg ctctccttgt taatttaagt gatattttgt ttgatattgt gaatataagc
    23461 gctggaagat aacgatatgg tgagcctatt cacataaatt aacattgtgt gttattttat
    23521 gtgaactaag cgttagttgc gccgcgcacg tttcgcaggc aaatagcgta gaatgtcagc
    23581 aggacaaagg aaggagcaaa agttgatacc cgtagagcgt cgccaaatca tccttgagat
    23641 ggtagctgaa aaaggcattg tcagtattgc tgaactaacg gacagaatga atgtatcaca
    23701 tatgaccatt cgtcgggatt tacaaaaact ggagcagcag ggagccgttg tgctggtgtc
    23761 cggaggcgtc cagtctccgg gacgcgtggc gcatgaacct tctcatcagg taaaaactgc
    23821 gctagcaatg acgcaaaaag cggctattgg caagctggcg gcaagtcttg ttcagccggg
    23881 aagttgtatc tatctggatg cgggaacgac cacgttagcg atagcacagc atctgattca
    23941 catggagtca ctgactgtgg tcacaaacga tttcgttatt gcgaactact tgctcgacaa
    24001 cagtaattgc acaattattc acactggcgg tgcagtgtgt cgggaaaacc gttcctgtgt
    24061 cggggaagcc gctgcgacca tgctgcgcag cctgatgatt gatcaggctt ttatttctgc
    24121 atcgtcatgg agtgtgcggg ggatttctac gccagcggaa gataaagtca cggtgaaacg
    24181 ggcgattgct agtgccagcc gccagcgagt tttggtctgt gatgcgacga aatatggtca
    24241 ggtggcgaca tggctggcgt taccattaag cgagtttgat cagattatta cagacgacgg
    24301 tttgccggag agtgccagtc gcgcgctggc gaagcaggat ctctctttgc tggtagcgaa
    24361 aaatgaataa tggcctgcaa taacatctgg ttactcatgc ttcacagaag aagcatgaga
    24421 ctaccttatt ttataaaatg acagccgccc acttttcggc gatccggtat caatataaat
    24481 ctggttagcg aacgtctgaa tgttatcaaa catcatatgt ccaaatataa aataatcagc
    24541 gccgtttatt tgttgtaact cgccattaag cgatttctgc acacgataaa caggccagag
    24601 taattcgctc tccgctattt ctttgccaaa gagatattca tcacccggat aatctgcatg
    24661 tgcgatgaca tattttatta tgtcgttagt gatttcaata atatgtggaa ggtgatggaa
    24721 tttcagcaac agatctattg cctcttgttg ctctgaatca tttaaatcga aaaaccagtc
    24781 accaccgctg gcaagccaca tattgccatc gccggtcgcg aacgcatcca gctccattgc
    24841 ttcgtggttg cctttaacgg agataaacca gggctggttt agcaggcgca ggacgtcaag
    24901 actctccggc ccacgatcaa tattatcgcc gacagaaata agtaagtcgg tttcagggca
    24961 aaaagagagt tgatgtaagc gggattgtaa taattgatat tcaccatgaa tatcaccaac
    25021 gacccatata tggcgatagt gatgggcatt aattttttga tagcgtgtag atggcatggt
    25081 tttaccctgt aaaataagct ttcctattat acagggggtt tttattttat tcgtcagttg
    25141 tcgttaatat tcccgatagc aaaagactat cgggaattgt tattacacca ggctcttcaa
    25201 gcgataaatc cattccagcg cctgacgcgg agtcagtgaa tccgggtcca ggttttccag
    25261 agcttcgacc gctggcgagg tttcttccgg tactgacagc aaagacattt gcgtaccatc
    25321 cacttgcgta gcggcggcgt tcggcgaaat gctttccagc tcacgcagtt tttgccgtgc
    25381 gcgcttaatc acctcttttg gcacgcctgc cagagctgca accgccaggc cgtagctttt
    25441 gctcgccgcg ccatcctgca cgctatgcat aaaggcaatg gtgtctccgt gctccagcgc
    25501 atcgagatgc acgttggcga cgtcttccat tttctccggt agctgggtca gctcgaaata
    25561 gtgggtggca aacagcgtca atgccttaat cttattcgcc agattttctg cgcacgccca
    25621 cgccagcgac agaccatcgt aggtggacgt tccacgcccg atctcatcca ttaacaccag
    25681 actgtattcg gtggcgttat gtaaaatatt ggcggtttca gtcatctcca ccataaaggt
    25741 tgagcgcccg gaagccaggt catctgccgc gcctacgcgg gtaaagatgc gatcgatagg
    25801 tccaatctcg actttttgtg ccggtacata gctgccaatg taggccatca gcgcaatcag
    25861 tgcggtctgg cgcatatagg tacttttacc gcccatgttc gggccggtaa taatcaacat
    25921 acggcgctgc ggcgacagat tcagcgggtt agcgataaat ggctcattca gtacttgttc
    25981 aactaccgga tggcgacctt cggtaatgcg aatgcccggt ttatcaatga aggtcgggca
    26041 ggtgtagttc agggtatagg cccgttccgc caggttaacc agcacgtcga gttccgccag
    26101 cgcgctcgcg ctctgttgca acgcttccag atgcggcaac agcaggtcga acagctcttc
    26161 ataaagctgt ttttccagtg ccagtgcttt gccttttgag gtgagaactt tatcttcgta
    26221 ctcttttagc tctggaatga tgtagcgctc ggcgtttttc agcgtctggc gacgcatgta
    26281 gttgatgggt gccagatggc tttgcccacg gctgatttga atgtagtagc cgtgcaccgc
    26341 attaaagcca actttcagcg tgtccaggcc ggtacgttca cgctcgcgga cttccagacg
    26401 ctccagataa tcggtcgcgc cgtcagccag cgcgcgccac tcatccagct cttcgttata
    26461 gcccgatgcg ataacaccac cgtcgcgtac cagcaccggc ggtgtgtcga tgattgctcg
    26521 ctccagcaga tcgcgcagct cggcaaactc gcccatcttc tcacgtagcg cctgtaccgg
    26581 tgcactatcg acagtttcta actgtgcacg cagctccggc agttgctgga aagcgtggcg
    26641 catacgggcc agatcgcgtg ggcgagcagt tcgtaaagcc agacgtgcca gaatacgttc
    26701 aaggtcgccg acctgacgca gtaccggctg tagcccggcg gtgaaatcct gcaatgcgcc
    26761 aatagtttgc tggcgctcaa gcaacacgcg ggtatcgcgc actggcatat gcagccagcg
    26821 tttcagcata cgactaccca tcggcgtgac ggtgcagtcg agcacagaag ccagcgtatt
    26881 ttccgcaccg cccgccaggt tctgagtaat ttccaggtta cgacgtgtcg cggcatccat
    26941 aatgatgctg tcctgctcac gttccatggt gatagaacga atatgcggca gggtcgtgcg
    27001 ttgggtatct ttcgcatact gcaacagaca accggcagca caaagtccgc gcggcgcgtt
    27061 ctcgacgcca aaaccgacca gatcgcgggt gccaaattgc agattcaact gctggcgcgc
    27121 ggtgtcgatt tcaaactccc acagcgggcg acggcgcagg ccgcgacggc cttcaatcag
    27181 cgacatctcg gcgaaatctt ctgcatacag cagttccgcc ggattagtgc gttgcagctc
    27241 tgccgccatc gtttcgcggt cggccggttc gctcaggcga aaacgcccgg agctgatatc
    27301 cagcgtcgcg tagccgaaac ctttgctgtc ctgccagata gccgccagca ggttgtcctg
    27361 acgctcctgt aacagggctt catcgctgat ggtgcctggc gtaacgatac gcacaacttt
    27421 gcgctcaacc ggacctttgc tggtcgccgg atcgccaatt tgttcgcaga tggcaacgga
    27481 ctctccctga ttcaccagtt tggcgagata gttttccacc gcatggtagg gaatccccgc
    27541 catcgggatc ggctctcccg ccgaagcacc gcgtttggtc agtgaaatat ccagcagttg
    27601 cgacgcgcgt tttgcgtcgt cataaaacag ttcataaaaa tcacccatcc ggtaaaacag
    27661 caggatctcg ggatgctggg ctttcagcct gagatactgc tgcatcatgg gcgtatgggc
    27721 gtcgaaattt tctattgcac tcatggggtt atgtccggtt ccctgatatt aaatggggtg
    27781 tgatggtttt tattttcgtt gtaatcacat aaggcgcaca tgataccgga gttaatcatt
    27841 tcaggggaag aagcgaacgg cgagttcttg atgctggttc cgggaaagat gctgttttgc
    27901 agcttttgcg tgatgcttat gaagcaagta cgcaaaattg cgtcggttga ataaacctcc
    27961 tccttcagag ccatagtccc ctttcaaatt aagttgtaat ataaatacac cttaagagta
    28021 tggaggcagc atgtccggca agcgtatctc tcgtgaaaaa ctgacgatta aaaaaatgat
    28081 cgatctttat caagcgaaat gcccgcaggc gtcagcggag ccggagcatt acgaggcgtt
    28141 gtttgtttac gcgcaaaagc ggctggataa atgtgtgttc ggcgaagaga aaccagcctg
    28201 taagcagtgt ccggtgcact gttaccagcc tgcaaagcgt gaagagatga aacagattat
    28261 gcgttgggcc gggccgagaa tgctgtggcg tcatccgatc ttaaccgtgc gtcatctcat
    28321 cgatgacaag cgtccggtgc cagaactgcc ggaaaaatat cgtccgaaaa aacctcgtga
    28381 atagtatgcc ggacaggcag atctgtccgg caatttgcag ttaaatcaat gccgatttat
    28441 caattcccag ccgcttcatc cgtgacagca gggtcgtgcg tttcagcccc agacgttgcg
    28501 cagcgccttt aggcccggca accacgccgt tggtttcttt cagcacgcga acaatcaact
    28561 gatattcatc ttcgccctcc tgagcgacaa ccgttgcggc aggcggcgtt tcaggctccg
    28621 gtaaagcaat atctggcaat gacagctgca gcacgttgcc gcgtgttagc aatactgcgc
    28681 gctcaatgac gttttccagt tcgcgtacgt tacccggcca ctccatgttg ctcaaggtgc
    28741 gcagcgtctc ggctggaatg ctgtcgatat tgcgccccag acgacgggca attttgaagg
    28801 taaaggcttt cgccagcagc ggaatatctt ccggacgctc gcgtagtggc ggcaggtgaa
    28861 tcgggaatac gttcaggcgg taatagagat cgctacggaa ctctcggtcg gcgaccattt
    28921 ttttcagatc gcggttagtc gcggcaatta aacgtacgtc cgtctgaatg attttgttgc
    28981 tgccgagacg ttcaaactcc tgttcctgca atacacgcag caacttcggc tgtaactcca
    29041 gtggcatatc gcccacttcg tcgaggaaca gggagctttt atccgccagt tcaaaacgac
    29101 caatgcgctg ggcgctggca ccggtaaaag ccccacgctc atgaccaaac agatcgcttt
    29161 ccagcaatcc ggcaggcatc gccgcgcagt tcattttgac catgcggcga ttattacgcc
    29221 cactgagatt atggatcgcg cgggcaatca gctctttacc cgtgccagtt tcaccgagga
    29281 tcagcacggt actgtcactt tgcgccacca tttcaacttg tttaagcacg ctgtacatgg
    29341 cttcgctgcg gccaataatc tcgccaaatt cactatcaac attgttgagc tgctcggtca
    29401 gggcgaggtt ttcatcaacc agccgttctt tcagacgatg gatttcctga taggcgaggg
    29461 cgttatcgac agcgattgcc acacgttcgg caatctggcg cagcaaattc agattggtag
    29521 tggtaaacac tttctcttcg cattgcgcca gtttcagcac gcccagcatg gtgtcgccag
    29581 acatcagcgg taacaggcac aaggtttgaa tctggttgcc ccaggtgtcg aacaacatgc
    29641 gttcataggg ggctaaatcg tcccgctcgt ggagattgat cagcagcatc tctttacttt
    29701 tgaacacgcg ttcggtgagg gttccggctt catcgacttc gctctgttcg tgggcgggat
    29761 gttgtttatc aagatagtga gtggagtaga tgttgagttt gtttttacgg tggctgcgta
    29821 agacgatact gatatcgtca atgtcgaaat agtaatggat ttctttggcg acttcgctga
    29881 ccagttcgtc catatccagg cgggaaagca ccgcgttggt aatggcaacc aggatgcgga
    29941 agttatcgcg ctcccggcat aacaactcat agtcgacatt gttgacgacg cggctctgga
    30001 tttgttcggt gacgacagaa acgatctgcg taaatgtttg cagacgattg aactcttttt
    30061 cgctccaggg acgatcgtca taacgaataa attcacagcc accaaaaata tgcccttccg
    30121 ccgccagtgg catcaggcaa tagtgaccaa attaggggta tagcccacca gcgaccagct
    30181 gcggccaggt ttcacaaaat tcttcgtaac tgcaatgcag cgtatcaggg cgcgacaaaa
    30241 tgcggcgtac cggaccgtgt gccagaacag tttcgtcttc atatttaatg ggggtgtctt
    30301 tttcacgcga tgcgtaataa gacgcacgtt gagtctgcgc ttgccacaac acaatagccg
    30361 cgttgtcggc gagcgcagaa cgctttacca gttgcgaaag agcctcacac agcgaggcca
    30421 gatcgggctg ctgcaatagt gtccgagtga tgtcgaacaa cccttgctgt ccgagatcac
    30481 tcatcggtgt atatgacatt ttgttcttcc aggaaagcac cgcaaagcgg tgcaaaaggt
    30541 cattgactaa ctataggaga tttagaattt tattagcata tacgcggaag cggttcggcg
    30601 tgtggtaaat cgagggttcg tttcacacca tacagaccgg caagacgaac acctttacgt
    30661 tccaccactt caccaattag cgccgcgtct ttccccagtg gatgggaatg taacgctgcc
    30721 agcacttgct ctgctgcgtt gcgttcaaca gctattacta gtttgccttc gttggcaaag
    30781 ttaagggcgt ccagtcccag cagttcgcaa acgccgcgca cagcaggttt aaccggcagt
    30841 gctgattcag aaatttcaat accacaaccg caggctgccg cgaactcatg aaccaccgcg
    30901 tttacaccac cacgggtggc atcacgcagc gctttcacgc cgggaatgtc acgcagcgtc
    30961 tgaataagcg gcgtcagcac cgcgcagtcg ctgaccagtt caccatccag ccccagttgt
    31021 tcacgcaggt taaggatagt cgccccgtgg tcgccgagtg taccgctaac cagcaataca
    31081 tcgcctgcgg ttagcgtctg tgcgccccag tgaatattcg ccggaattgc gcccatgcca
    31141 gcggtgttga taaacagttt atctgccgcg ccgcgctgca ccactttagt atcgccagta
    31201 acgatggcaa tgcctgccgt gcgggcggtt tctgccatgc tggtcactac ggctttcagt
    31261 gtctccatcg gcaatccttc ttcgaggata aagccacagg agagatagca cggaatagcg
    31321 ccactgaccg caacgtcatt cgcggtgccg caaatcgcca gcttgccgat attaccgcca
    31381 ggaaagaaca gcgggtcaat gacgtaactg tcggtggaga acgccagacg atcgccttct
    31441 gctaccagct gcgccagatc aagacgtgcc tggtcttcct gctctgccag ccacgggttg
    31501 gcaaaggctt ccataaacag gctgttgatt aattgctgca tcgcctggcc accgctgccg
    31561 tgggcgagtt ggatattatt cacgcttcac tctcctgctg acgatactga taccacgcgg
    31621 cgcacgctcc ttcggaggag accatcagtg caccaaatgc ggtttgagga ttacaggtgt
    31681 taccaaacag cgggcattga tgcggcttac atttgcccgt taatacctca ccacaacgcg
    31741 cgcgcgggtc atcgcagacc tgctgcggtg ccgggcggaa atgtgcttcg gcatcgaatc
    31801 gttgataatc cggcgtcagg tgcacgccag aagattcaat cacgcctaag ccgcgccatt
    31861 cgctgtcgcc gttgacacag aacacatcgg caatcgcctg ttgcgccagc aggttaccgg
    31921 catccggtac cacccgacga tactgattct ctaccttgct gtgggccgct attttctgct
    31981 ccaccagcat caccacgcct tgcagtagat caaggggttc gaaaccagcg accaccagcg
    32041 gacgatgaaa atcgctggcg ataaaattat aggcatcagt gccgataacc atactaacgt
    32101 ggcccggcgc gaggaacgcg tcgataccgt tatccggctg ttccagcaaa ctgcgcagcg
    32161 tcgggataag cgtaatgtgc tggcagaaga agtaaaaatt ctgcacatca cgcgctttcg
    32221 cctgttgcag agtgatggcg gtggtcggca tggtggtttc aaaacctaag ccgaagaaca
    32281 ccactttgcg ggttggattc tcctgcgcca gtttcaacgc atccatcggc gagtaaacga
    32341 tgcgcacatc ggcaccgcgt gcttttgcct gcaacagcga tccctgtttc cctggtacgc
    32401 gcatggcatc gccaaaggta cagaagatga cttccggatg gctggcaatc tccacgcagg
    32461 tgtcgattct acccatcggc agtacgcaca ccgggcaacc cggaccgtgg ataaactcaa
    32521 cgttttcagg cagtaactgg tcgaggccga atttaaaaat agcgtgggta tgaccaccgc
    32581 acacttccat aatccgcaga gggcgttcgg cggtgtaaga gagatgtgaa gcacgttcgc
    32641 gcagatgctc aattaactgc atcacctgtt ccggcgcgcg atattcatca acaaaacgca
    32701 ttatttttcc tcgccataca acagcgcgcc gacatccggc tcaacgtcaa acatgttttg
    32761 taaggcgtcg agagtgtcgc gtgcttcggc ttcagtaatt acgctcatgg caaagccaac
    32821 gtgtaccagt acccactggc ccacgcgcgg ctgaccgttt tcatcgcagc tgccgactaa
    32881 cgttaaatcg acatcgcgct gaatgccgca gacgtcgact tttgcctggt tgccgtcaat
    32941 ggtgcggatc tggccgggaa cgcctatgca catcgctgtg tctccagcca gttcagccac
    33001 tggtccatcc cttcaccgct ggtggcggaa ataaggatga tttcaatttc tggattgact
    33061 tcgcgggcgc aggcgatgca cttctcaacg tcaaagttga gatacggcaa caggtcaact
    33121 ttgttgagca gcatcagcga ggcggcggca aacatatgcg gatatttcag cggtttgtct
    33181 tcaccttcgg taacggaaag caccgccact ttgtgttttt caccgagatc gaagctggcc
    33241 gggcatacga ggttgccaac gttttcgata aacagaatac cgttatcgtc cagtggcaga
    33301 cgcggtgcgg cgtcggcaat catctgtgcg tcaagatggc agcctttacc tgtgttcacc
    33361 tgaatcgctg gtgtgccggt agcgcgaatg cgtgcggcat cgttcacggt ttgctggtca
    33421 ccttcaataa ccgcgcacgg aacgctgtct ttcaggcgca ttagggtttc cgtcagcagg
    33481 gtggttttac cggaacctgg gctggaaacc aggttgagca ccagttgctt gcgggcagca
    33541 aagcgcgcgc ggttgcgttc agccagacgg ttgtttttgt ccagcacgtc aatttcgact
    33601 tccagcatcc gacgctggct catgcccggt gcgtgagtgc ccgcttcgcc atgaccgtaa
    33661 tgcaggtcgc cttcttcagt ctggctgggg gtaaattcag gcgctttaat gccggtgatt
    33721 ttcatcttcg ggcgtgccgc cggggcaaat ggcgcgctac gaaacgcgga atgagggtta
    33781 tgttcatcac cctcgatata caggttgcct tcaccgcaac cgcatgttgt acacatcgct
    33841 cactcctggt ctatttctat ccgccgaatc tgtaaaccgt cgtctgccac aatctgtagc
    33901 atgtcaccat gacactgtgg acagcggcgg acgcgctggg tcaatagcgt cacatactgt
    33961 tggcatgttt cacaccagca ttcgacctct tgttcttcga ggtgcagttt acaaccttcc
    34021 gccacgctgc cgcggcaaac cagatcaaaa caaaaggcaa gagagctggt ttcgacacaa
    34081 gaaaatgcgc caattttgag ccagacccca gttacgcgtt ttgcgccgtg ttttgcggcc
    34141 tgctgttcga tcaattccag tgcccgttgg cagagggtta tttcgtgcat atcgcctccc
    34201 attaactatt gccagctaca agcaataatt gtgccagtgt tgattatccc tgcggtgaat
    34261 aatgtcgatg atgtcgaaat gacacgtcga cacggcgacg aaattcatct ttagcttaaa
    34321 aatcgtttta ataacaataa attaaaaatt ggcacagaaa atgcttaaag ctggcatctc
    34381 tgttaaacgg gtaacctgac aatgactatt tgggaaataa gcgagaaagc cgattacatc
    34441 gcacagcggc atcgtcgcct acaggaccag tggcacatct actgcaattc gctggttcag
    34501 gggatcacgt tatcgaaagc gcgcctgcat cacgccatga gctgcgcgcc ggacaaagaa
    34561 ctctgtttcg tcctttttga acattttcgt atttacgtca ccctggcgga tggctttaac
    34621 agccacacca tcgagtatta cgtcgaaaca aaagagggcg aagacaaaca gcggattgcg
    34681 caggcgcaac tgagcattga cggcatgatt gatggcaagg tcaacatccg cgatcgcgaa
    34741 caggttctgg aacactatct cgaaaaaatc gctggcgttt acgacagctt atacaccgcc
    34801 attgaaaaca atgtgccggt gaatttaagc caactggtaa agggacaaag cccggcagca
    34861 tgagctgagg ctttgcccgt tttgcaggcg ttacgcctgt ttggggatgg gcgtgtcgat
    34921 gagtgtcgaa aatgacattt catcggcatg ttttcgtcaa aaatgacaat cacctgagga
    34981 atgcctggtg aatcgttttg taattgctga ctccacgctc tgtatcggct gccacacttg
    35041 tgaggcagcc tgttcagaga cgcatcgcca gcacggcctg caatcaatgc cgcgcctgag
    35101 agtgatgctg aatgaaaaag aatctgcgcc gcagctctgt caccactgtg aagatgcacc
    35161 ctgcgcggtg gtctgcccgg ttaacgtcat cacccgcgtt gatggggccg tgcagttgaa
    35221 tgaaagcctg tgcgtaagct gcaagctgtg cggcatcgcc tgcccgtttg gcgcaattga
    35281 attttccggc agccgtccgc tggatattcc tgcaaatgcc aataccccga aagcgccacc
    35341 ggcaccgcct gctccggcgc gtatcagtac gctgcttgac tgggtgccgg gtattcgcgc
    35401 gatcgccgtc aaatgtgacc tttgtagctt tgatgaacaa ggtccggcct gcgtgcggat
    35461 gtgcccgacc aaagccctgc atctggtgga taacaccgat atcgcccgcg tcagcaaacg
    35521 taagcgtgag ctgactttta atacggactt tggcgatctc accttgtttc agcaggctca
    35581 aagtggagag gctaaatgag cgcaatttcc ctgatcaata gcggcgtggt gtggtttgtc
    35641 gccgccgctg ttctggcatt tctcttttct tttcaaaaga cgctaagtgg ctggatagcc
    35701 ggaattggcg gcgcggttgg tagcctgtat acggcagccg cgggcttcac tgtgctgact
    35761 ggcgcggttg gcgtgagcgg tgcgctgtcg ctggtaagct acgatgtgca aatctccccg
    35821 cttaacgcga tttggctgat tacgctcggt ctgtgcggtc tgtttgtcag cctctacaac
    35881 attgactggc atcgccacgc gcaggtgaag tgcaacggct tgcagatcaa tatgttgatg
    35941 gctgctgccg tctgcgccgt catcgccagc aacctcggca tgttcgtggt aatggccgaa
    36001 gtcatggcct tgtgtgcggt gttcctcacc agcaacagca aagagggcaa actgtggttt
    36061 gcgctggggc gtcttggcac tctgctgctg gcgattgctt gctggctgct gtggcagcgt
    36121 tatggcacgc tggatctgcg cctgctggat atgcgtatgc aacagctgcc gctcggttcc
    36181 gatatctggc tgctcggtgt gattggcttt ggcctgctcg ccgggattat tccgctgcac
    36241 ggctgggtgc cgcaggcaca tgctaacgcc tctgcgccag ctgccgcgtt gttttctacg
    36301 gtagtcatga aaattggcct gctgggcatt ttaatcctgt cactgctggg cggtaatgca
    36361 ccgctgtggt gggggatcgt gctgctggtg ctcggcatga tcaccgcgtt tgtcggtggt
    36421 ctgtatgcgc tgatggagca caacatccag cgcctgctgg cttaccacac cctggaaaat
    36481 atcggcatta tcctgctggg gctgggcgct ggcgtaacgg gtatcgcgct cgaacaaccg
    36541 gcgctgattg ctcttggcct ggtcggtggt ctgtaccatc tgcttaacca tagcctgttc
    36601 aaaagcgtac tgttcctcgg ggcggggagc gtctggttcc gtaccggtca tcgcgatatc
    36661 gaaaaactcg gtggtattgg caagaaaatg ccggttatct ccatcgccat gttagtcggg
    36721 ttgatggcaa tggctgcgct gccgccgctg aacgggtttg ccggggaatg ggttatctat
    36781 caatcctttt tcaaactgag caatagtggc gcgtttgttg cccgtctgct ggggccgctg
    36841 ctcgctgtgg gtctggcaat taccggtgcg ctggcggtga tgtgtatggc gaaagtctat
    36901 ggcgtcacgt tcctcggcgc gccgcgtacc aaagaagccg aaaacgccac ctgtgcgccg
    36961 ctcctgatga gcgtaagcgt agtggcactg gcgatttgct gcgtaattgg cggtgttgct
    37021 gcgccgtggc tgctgccgat gctctctgct gccgtacctc tgcctctgga gcctgctaac
    37081 accaccgttt ctcaaccaat gatcacattg ctgctgattg cctgcccgct gctgccattc
    37141 atcattatgg cgatttgcaa aggcgaccgc ctgccgtcac gttcccgcgg tgcggcctgg
    37201 gtgtgcggct acgaccatga aaaatcaatg gtgattaccg ctcacggttt tgccatgccg
    37261 gttaaacagg cgtttgcgcc agtgctgaaa ctgcgcaaat ggctgaatcc ggtgtcgctg
    37321 gtgccgggct ggcagtgcga aggtagtgcg ttgctgttcc gccggatggc gctggttgaa
    37381 ctggcggtac tggtggtgat tattgtttca cgaggagcct gagaatgagt gttttatatc
    37441 cgttaattca ggcgctggtg ttatttgccg ttgcgccgct gctctccggt attacccgcg
    37501 tggcgcgcgc ccgtctgcat aaccgtcgcg ggccgggcgt gttgcaggag tatcgcgaca
    37561 ttatcaaact gctgggccgc cagagcgttg gcccggatgc ctctggctgg gtgttccgcc
    37621 tgacgccgta tgtgatggtg ggcgtcatgc tgactatcgc tactgcgctg ccggtggtga
    37681 ccgtcggttc tccgctgccg caactgggcg atttgatcac cttactgtat ctcttcgcca
    37741 tcgcgcgttt cttctttgcc atttccggtc tggataccgg tagcccgttt accgctatcg
    37801 gtgcgagccg tgaagcgatg cttggcgtgc tggtcgaacc gatgttgctg cttggtctgt
    37861 gggttgccgc acaggttgcc ggttccacca acatcagcaa cattaccgac accgtttatc
    37921 actggccgct gagccaaagc atcccgctgg tgctggcgct ctgcgcctgt gcgttcgcca
    37981 cttttatcga aatgggcaaa ctgccgttcg acctggcgga agccgagcag gagttgcagg
    38041 aaggtccgct ctctgagtat agcggcagcg gctttggcgt catgaaatgg ggtatcagcc
    38101 tgaaacagct ggtggtgttg cagatgttcg tcggggtgtt tattccgtgg ggacaaatgg
    38161 aaaccttcac cgccggtgga ctgctgctgg cgctggtgat tgcgatcgta aaactggtgg
    38221 tcggcgtcct ggttatcgcg ctgttcgaaa acagcatggc ccgtctgcgt cttgatatta
    38281 cttcgcgcat tacctgggct gggtttggct ttgcattttt agcgttcgtc tccttgctgg
    38341 cggcgtgatt aaagagagtt tgagcatgtc tgaagaaaaa ttaggtcaac attatctcgc
    38401 cgcgctgaat gaggcatttc cgggcgtcgt gctggaccac gcctggcaga ccaaagagca
    38461 gctgactgtc accgtaaagg tgaactatct gccggaagtg gtggagtttc tctactacaa
    38521 gcaggggggc tggctgtcgg tgctttttgg taacgacgaa cgcaaactga atggtcatta
    38581 cgccgtttat tacgtgctgt cgatggagaa ggggactaag tgctgggtaa cggttcgcgt
    38641 cgaagttgat gccaacaaac cggagtatcc gtccgtgacg ccgcgcgttc cggcggcggt
    38701 gtggggcgag cgcgaagtgc gcgatatgta cggtttgatt ccggttggtc tgccggatga
    38761 acgccgtctg gtgctgccgg atgactggcc ggatgaactt tatccgctgc gtaaagacag
    38821 catggattat cgtcagcgtc cggcaccgac caccgatgct gaaacctacg agttcatcaa
    38881 cgaactgggc gacaagaaaa acaacgtcgt gccgattggt ccgctgcacg tcacttctga
    38941 cgaaccgggc cacttccgtc tgttcgtcga tggcgaaaac attatcgacg cagactaccg
    39001 cttgttctat gtccatcgcg gtatggaaaa actggcggaa acccgcatgg gttataacga
    39061 agtgaccttc ctctctgacc gtgtgtgcgg gatctgcggc ttcgcccaca gcaccgccta
    39121 caccacgtcg gtggaaaacg cgatgggtat tcaggtgcca gaacgtgcgc agatgatccg
    39181 cgccattctg ctggaggtgg aacgcctgca ctcgcatctg ctcaacctcg gcctcgcctg
    39241 ccactttacc ggcttcgact ccggctttat gcaattcttc cgcgtgcgtg aaacctccat
    39301 gaaaatggcc gagatcctca ccggtgcgcg taaaacctac ggcctgaacc tgatcggcgg
    39361 gattcgtcgc gatctgttga aagatgacat gatccagacc cgccagctgg cgcaacagat
    39421 gcgtcgtgaa gtgcaggagc tggtggatgt gctgctgagc acaccgaaca tggaacagcg
    39481 caccgtcggc attggtcgtc tggacccgga aattgcccgc gatttcagta acgttggccc
    39541 gatggtccgc gccagcggac acgctcgcga tacccgcgct gatcacccgt ttgttggtta
    39601 tggcctgctg ccaatggaag tccacagcga gcagggctgc gacgttattt cgcgtctgaa
    39661 agtgcgtatc aacgaagtct ataccgcgct gaacatgatc gactacggtc tggataacct
    39721 gccgggtggc ccgctgatgg tggaaggctt tacctacatt ccgcaccgtt tcgcgctggg
    39781 ctttgccgaa gcgccgcgcg gtgatgatat ccactggagc atgactggcg acaaccagaa
    39841 gctgtaccgc tggcgctgcc gtgccgcgac ctacgcgaac tggccgaccc tgcgctacat
    39901 gctgcgtggc aacactgttt ccgatgcgcc gctgattatc ggtagcctgg acccttgcta
    39961 ctcctgtacc gaccgcatga ctgtggtcga tgtacgtaag aagaagagca aagtggtgcc
    40021 gtacaaagaa ctcgagcgct acagcattga gcgtaaaaac tcgccgctga aataaggaat
    40081 cgccatgttt acctttatca aaaaagttat caaaaccggt acggcgacct cgtcttatcc
    40141 gctggagccg attgcggttg ataaaaactt ccgtggtaag ccagagcaga acccgcagca
    40201 gtgcatcggc tgcgcggcct gcgtcaatgc ctgcccgtca aacgccttaa cggttgaaac
    40261 tgacctcgcc acaggagagc tggcctggga gtttaatctc gggcgctgca tcttctgtgg
    40321 acgctgcgaa gaagtttgcc cgacggcggc gattaagctg tcgcaagagt acgaactggc
    40381 ggtgtggaag aaagaagact tcctgcaaca gtctcgcttc gcgctgtgca actgtcgcgt
    40441 ctgcaatcgt cctttcgccg tccagaaaga gatcgactac gccattgcgc tgcttaagca
    40501 caacggcgac agccgcgcgg aaaaccaccg cgaaagcttt gagacttgcc cggaatgtaa
    40561 gcgccagaaa tgcctggtgc cgtccgaccg tattgaactg actcgccata tgaaagaggc
    40621 catctgatga gcaatttatt aggcccccgt gacgccaacg gcattccggt cccgatgacg
    40681 gtggatgaat ccatcgccag catgaaggcg tcgttactga aaaaaatcaa acgttctgcc
    40741 tatgtttacc gcgtggactg cggcggctgc aacggctgcg aaatcgaaat tttcgccact
    40801 ttatcgccgc tgtttgatgc agaacgcttc ggcattaaag tcgttccttc accgcgtcat
    40861 gcggatattt tactgtttac cggcgcggtc acccgtgcaa tgcgatcccc tgcgctgcgt
    40921 gcgtggcagt ccgcgccgga cccgaaaatt tgtatctcct acggtgcctg cggtaacagc
    40981 ggcgggatct tccacgatct ctactgcgtg tggggcggta cggataaaat tgtccctgtg
    41041 gatgtttata tccctggctg cccgccaacg cctgccgcca cgctgtacgg ctttgcaatg
    41101 gcgctcggcc tgctggagca gaaaattcac gcccgtgggc cgggtgaact ggatgaacaa
    41161 ccggcggaga tcctgcatgg cgatatggtg cagccgctgc gcgtgaaagt ggatcgcgaa
    41221 gcacgccgcc tggcgggtta tcgttacggt cgtcagattg ccgatgactt cctgacacag
    41281 ttagggcagg gcgaagaaca ggttgcacgc tggctggaag cggaaaacga tccgcgtctg
    41341 aacgagattg tcagccatct gaatcatgtt gtagaagagg tgcgtatccg atgagtgaaa
    41401 aggtggtgtt cagtcaactg agccgtaaat ttattgatga gaacgatgcc acgcccgccg
    41461 aggcgcagca ggtggtctat tacagcctgg cgattggtca ccaccttggg gttatcgatt
    41521 gcctggaagc ggcgctcacc tgcccgtggg atgaatatct ggcatggatt gccactctgg
    41581 aggcgggcag tgaagcccgc cgcaaaatgg aaggcgtacc gaagtacggt gagatcgtca
    41641 tcgacattaa ccatgtgccg atgctggcca acgcattcga taaagcccgg gcagcgcaaa
    41701 cttcgcagca gcaggaatgg agtacaatgc tgttaagtat gctgcatgat attcatcagg
    41761 aaaacgccat ctatttgatg gtgaggagat tgcgtgactg acgttttact ctgtgttggc
    41821 aatagcatga tgggcgatga tggcgcaggt ccgctgctgg cggaaaagtg cgccgccgcg
    41881 ccgaaaggta actgggtggt gattgacggc ggtagtgcac cggaaaacga catcgtcgcc
    41941 atccgtgaac tgcgccctac gcggttactg attgtcgacg ccacggatat ggggctaaac
    42001 cccggcgaga tccgcatcat cgacccggat gatatcgccg agatgtttat gatgactacc
    42061 cataacatgc ctctgaacta tcttatcgac cagttgaaag aagatattgg cgaagtgatt
    42121 ttcctcggca tacagccgga tatcgtcggc ttttactacc cgatgaccca gccgattaaa
    42181 gatgcggtag aaaccgttta tcaacgactg gaaggctggg aagggaatgg cggcttcgcg
    42241 cagttagcgg tggaagaaga gtagtttttc attaaggaat caggacaggg atgttcttga
    42301 tggggtgaac cagctctgat gccaaatgct aaattgcccg atgcgctgcg cttatcgggc
    42361 cttcctggtt cgtgcgacat gtaggccgga taaggcgttc acgccgcatc cggcactgtt
    42421 acttactcta aatcttcccc attactggca atcacttttt tataccacca gaacgatttc
    42481 ttacgtgtgc gcgtcagcgt gccgttgcct gcgtcgtcac ggtctacgta gacaaaaccg
    42541 tagcgtttgc tcatttcacc cgtagaggcg gaaactaaat caatacagcc ccatgtggtg
    42601 tagcccatca gcggaatgcc atctgcaatc gcttcgccca ttgcgcggat atgttcgcgt
    42661 aaatagctga tgcgatagtc atcgttaatc tcgccattgg cagcaagttc atcttttgcg
    42721 ccgaggccgt tttccaccag aaacagcggc ttctgataac gatcgtacat catattcatg
    42781 gtgatacgca gaccgagtgg atcaattccc cagccccagt cgctcacttg cagatacggg
    42841 ttacgcagcg atttcaccac gttcgccgca ctgctgttgt tggcgttcat ctccgccgag
    42901 gcgcagcgcg aggcgtaata gctgaaagag acaaaatcga cggtgttttt caggatctca
    42961 tcatcgccca gcgctttatc tatggttacc cctttttcgc gaaatacgcg ggcagagtaa
    43021 gccggatacg cgccgcgcgc ctgcacatcg ataaaaaaca ggttttcccg atctttctcc
    43081 agcgccgccc agacatcttc cggtttgcag ctgtaagggt agaagttgcc gcccgccagc
    43141 atacagccca cctgattttg cgggttaacc tcatgggcga ttttggcggc tagcgcactg
    43201 gcaaccagct ggtgatgcgc ggcctgatat ttcacctgat cctgattttc accttcttca
    43261 aacaccagac ccgcgccgga gaacgggcta tgcaacataa tgttgatttc attgaaggtt
    43321 agccagtatt tcaccagacc atcaaatgct tcaaagcagg ttctggcgta gcggctgaaa
    43381 aactccacca gcttgcggtt acgccaggag ccatattcgg tgaccagatg catcggcaca
    43441 tcgaagtggc acaacgtgac cagcggttcg ataccgtact ttttacactc ttcaaagaca
    43501 gaacgataaa aagcaatgcc ctgctgattg ggcgtgattt catcgccctg cggaaagaga
    43561 cggctccagg caattgaggt acggaaaacc ttgaatccca tctctgccat cagggcgata
    43621 tcttctttat aacgatgata aaaatccgtc gcctcatggc tgggataaaa ctcgtcatcg
    43681 cgcaactgaa aacgtttttc cagccccagt ttcaccgcca ttcgatgctc gccgtgtggg
    43741 atcatatcga cggtggtcag cccttttccg ccttcacgga acgcaccttc agactggttg
    43801 gcggcaagcg cgccgcccca taaaaaacct tctggaaata ctgacatttt catcctcaat
    43861 taagacttac ttctttggcg acggtcggtt gtacgctctg atgctttcgc gcctgggcag
    43921 ccgcttcctc aacaggaata tcctcaaagc cgagcaacag tgtgaggata aacgacagca
    43981 ccaccgccag cgccatgacc gcgaacaccc agacgatgct cattggattc gccggatcga
    44041 agaattgcac gctggtaaat agccccggcg ctgccattga gtggctggca agccccgcca
    44101 taccggcaac cgcgccgcaa ataaaaccgc tgataagact ggcgataagc ggacgtttca
    44161 ggcggatcgc cacgccgtat aacgccggtt cggaaatccc cgccataatg gctgatgccg
    44221 ccgcagccag cgccgtctgg cgcagttccg ggtttttcgt tttccacgcc accgccagtg
    44281 atgaaccgcc cagcgacagg ttagcgccga tctctgacgg catgaccatc ccttctttgc
    44341 cggtttcggc aatggtctga atgatggttg gcgtaaagac gcggtgcatc ccggtcatta
    44401 ccagcagagg ccacagcgcg cccataatgg cgactgaaag ccagcccaga taaccatgaa
    44461 tgctgtaaac cagcgccgaa atggcgctac cgatccagat accaatcggg ccaatcagca
    44521 ggattgccag cggtgcggca atcaacacaa tcagcattgg cttgaggaag tttttggtca
    44581 ccgccggtgt aatgcgatcc acccagcgtt cgatatatga caggcaccag gtcatcacca
    44641 gcgccgggat cactgtgtag gtgtatttca ccgcggtgac cggaatcagg gcaaattcga
    44701 catgttcacc ctgggccgct ttcgccatca gttcaataaa actcggatgt accagcacac
    44761 ccgcgatggc aatcgccagc gacatattgg ttttaaattt gatggcggca gatgccgcga
    44821 ccatcagcgg caggaagaag aaagcaccgt caccaatcac attcagaatg gttaaggtcg
    44881 gcgatccttt tgtcagcacg ccgctcatct cgaggatcat cgccagcagt ttgaccatcg
    44941 atccgccgat aatcgccggg atcagcggtg acatggtgcc gatcagcgca tcgaggatcc
    45001 ccgcaccaat gcgacgcggc gtgagtttgg gtttacccac gggctgtgcg ggctgcatat
    45061 ctcccggcag caggctgacg acttcctgaa aggcttgtga aacggtattg ccgataatca
    45121 cctgacactg gttgtcacta cgtaccacgc cgagcacgcc ggggatggtt tttaacgtcg
    45181 ggctgtcgat aagctggtca tctttgataa caaagcgcaa acgcgtcata cagtgcgtga
    45241 ccgccgagat gttatcaacg ccgcccagtg ccgctatctc cgagcgtgcc agcgccgcat
    45301 aatttttggc catcggattt tatcctgtta tcagtagggt agatgtagtt gttatcagtc
    45361 gacgtaacgg aaaaatgccg atgctgaaac ggaattagga aaccggtttc acaaaatcat
    45421 gaatagttgt cactaaataa gcaagagagt taattattca ttttttatat ttgtgaaacc
    45481 ggtcacctga atatttttcc cgccgattgc gcaaggacaa tcggcaaacg gtgcagtaca
    45541 ctgcgttgat tgcgtgtaaa gggatgaaaa tgatgacgac gatgctggaa gtggcgaagc
    45601 gcgccggggt ttcaaaagcg accgtttccc gcgtgctttc aggtaatggc tacgtcagcc
    45661 aggagactaa agatcgcgtg tttcaggcgg tagaagagag cggttaccgc ccaaacttgc
    45721 tggcgcgcaa tctgtcggcg aagagtactc agacgctggg gctggtagtg accaacacgc
    45781 tttaccacgg catttatttt agtgaattac tctttcatgc cgcgcgaatg gcggaagaga
    45841 aagggcggca gttgctattg gcagatggta aacacagcgc cgaagaagag cgccaggcga
    45901 ttcagtatct gctggatttg cgctgcgacg ccattatgat ttacccgcgt tttttaagcg
    45961 tggatgagat cgatgacatc attgacgcgc acagtcagcc gataatggtg cttaatcgcc
    46021 gcctgcgcaa aaacagcagc catagcgtct ggtgcgatca taaacagacc agctttaatg
    46081 ccgtggcaga gttgataaac gccggacatc aggagattgc tttccttacc ggctcgatgg
    46141 attcccccac cagcattgaa cgtcttgctg ggtataaaga cgcgctggcg cagcatggta
    46201 ttgcgctcaa tgaaaaactt atcgctaacg gtaaatggac gcccgccagc ggggccgaag
    46261 gggtagaaac gttgctcgaa cgtggggcta aatttagcgc gttagttgcc agtaacgacg
    46321 atatggcgat aggtgcgatg aaagcgttgc acgagcgcgg cgtagcggtg ccagagcagg
    46381 tgtcagttat cggattcgat gatatcgcaa ttgcccccta tatcgttccg gcgctctcca
    46441 gcgtaaaaat tccggtgact gagatgattc aggaaattat tggacggctg atttttatgc
    46501 tcgatggcgg ggatttctca ccgccgaaaa ccttcagcgg aaaactgatc caccgcgact
    46561 cactcattgc tctttcgcga taaacattgt catcacatgt catcgtcact agtgtcgatg
    46621 acatcttttt tcctgcatta cagaaattaa tttccttaaa taacagtgaa ttaaaaactg
    46681 gcatgatttg tgaatgtatc ggcgcattaa ctgtcattgc tggagaattt gatgaaccgt
    46741 ttcatcattg ctgacgcgag taaatgtatt ggttgccgta cctgtgaagt ggcgtgcgtg
    46801 gtttctcatc aggaaaatca ggactgtgca tcgctgaccc cggaaacatt tttaccgcgt
    46861 atccatgtca ttaaaggtgt gaacatttcc acggcgacag tttgccgtca gtgtgaagat
    46921 gcaccgtgcg ctaacgtctg cccgaatggt gctatcagcc gtgataaagg gtttgttcat
    46981 gtgatgcagg aacgttgcat tggttgcaaa acctgcgttg tggcttgccc gtatggcgcg
    47041 atggaagtgg tggtacgtcc ggtgattcgc aacagcggcg cggggctgaa tgtacgggct
    47101 gacaaagccg aagccaataa atgtgacctg tgcaaccatc gtgaagacgg cccggcgtgt
    47161 atggcggctt gcccgactca tgcgctgatt tgtgtcgatc gtaataaact tgaacaactg
    47221 agtgcagaaa aacgccgccg tacggcgctg atgttctaaa aatcttacag aactgttctg
    47281 gcatcaaagg aattcccgga aatattcgta gaatacggag cgggcggtat cgccgacctg
    47341 tgactgccgg atgcgacgct gtcgtgtccg gctgggcggg ttacgcaatt catgatggcg
    47401 ggaataactc aatggcaaaa aacacatctt gcggtgtcca actgcgtatt cgtggcaaag
    47461 tgcagggcgt cggttttcgt ccgtttgtct ggcagctggc acagcaatta aatcttcacg
    47521 gcgatgtctg taatgacggc gatggcgtag aagtccggct gcgggaagac ccggaaacgt
    47581 ttcttgttca attgcatcag cactgcccgc cgctggcgcg tattgatagc gtcgagcgtg
    47641 agccgtacat ctggtcacaa ctgcccactg agttcaccat ccgccagagc gcgggaggtg
    47701 ccatgaatac gcaaattgtc ccggatgccg ctacttgccc tgcttgcctt gccgaaatga
    47761 ataccccagg cgaacggcgt tatcgttatc cgtttatcaa ctgtacccac tgcggcccgc
    47821 gtttcaccat tattcgcgcc atgccttacg accgcccgtt taccgtgatg gcggcgtttc
    47881 cgttgtgtcc ggcttgtgac aaagagtacc gcgacccgct cgatcgtcgc ttccacgccc
    47941 agccggtggc ctgcccggag tgtggcccgc atcttgaatg ggtaagtcat ggtgaacatg
    48001 cagaacaaga ggcggcatta caggcggcta tcgcacagtt aaaaatgggc aacattgtcg
    48061 ccatcaaagg gattggcgga tttcatcttg cctgcgatgc acgtaacagt aacgcggtgg
    48121 cgacacttcg ggcgcgcaaa catcgcccgg cgaaaccgct ggcggtcatg ttgccagtgg
    48181 ctgacggttt accagacgct gcgcgccagt tgcttaccac gcccgccgcg ccgattgtgc
    48241 tggtggataa aaaatacgtt cctgagcttt gtgatgatat cgcccctgac cttaacgaag
    48301 tcggggtaat gttgcctgcg aacccgctcc agcatttgct gttacaggaa ctgcaatgcc
    48361 cgctggtgat gacctccggc aacctgagcg gtaaaccacc agctatcagc aacgaacagg
    48421 cgctggcgga tttgcagggc attgccgacg gattcttgat acataaccgc gacatcgtgc
    48481 agcggatgga tgattcggtg gtgcgcgaaa gcggcgaaat gctgcgccgt tcgcgggggt
    48541 atgtgccgga tgcgctggct ttgcctccgg gctttaaaaa tgttccgcct gtgctgtgtc
    48601 tcggagcgga tctgaaaaat accttctgcc tggtgcgcgg tgaacaagcg gtgttgagtc
    48661 agcatctggg cgatttaagt gacgatggca tccagatgca gtggcgcgaa gcgttacgcc
    48721 tgatgcaaaa catctacgat tttaccccga aatacgttgt gcatgacgcg catccgggct
    48781 atgtctccag ccagtgggcg cgtgaaatga atctgccgac gcaaacggta ctgcatcatc
    48841 atgcccatgc agcggcgtgt ctggcagagc atcagtggcc gctggatggc ggtgatgtca
    48901 ttgctttgac gctcgacggt atcggtatgg gggagaacgg cgctttgtgg ggcggcgagt
    48961 gcctgcgggt gaactatcgc gaatgtgagc acctgggcgg cttgcctgca gtggcgcttc
    49021 cgggtggcga tttggcagcg aagcagccgt ggcgaaacct gctggcgcag tgcctgcgct
    49081 ttgtgccgga gtggcagaat taccctgaaa cagcaagtgt gcaacagcaa aactggagcg
    49141 tgctggcgcg ggccattgag cgtggaatta acgcgccgct ggcgtcatcg tgtgggcggt
    49201 tgttcgatgc agtggcggcg gcactgggct gtgcgccagc cacgttaagt tatgaaggtg
    49261 aagcggcttg tgctctggag gcgctcgcag cctcatgcca cggagtgacg catccggtga
    49321 cgatgccgct ggtggacaat caactggatc tcgccgcttt ctggcagcag tggctgaact
    49381 ggcaggcaca ggttaatcaa cgcgcgtggg cgtttcatga tgcgctggcg cagggttttg
    49441 ccgcgttgat gcgtgagcag gccacgatgc gtggtatcac tacgctggta tttagcggcg
    49501 gggttattca taaccgtttg ctgcgtgcac gtctggcgca ttatctcgct gatttcacat
    49561 tgctctttcc acagagttta ccggcgggtg atggcggttt gtctctgggg cagggggtta
    49621 ttgctgcggc gcgttggtta gcgggtgaag tccagaacgg ataaaacgtg ttgcatttgg
    49681 acaccattgc cggatgcggc gtaaacgcct tatccggcct acacatgcgt ccccgtaggt
    49741 cggataagac gcgctagcgt cgcatccgac attttaggca cagtagccca cctacatcgg
    49801 caatgttttc aacaatccaa atgcctcttt catccgatcc tcactgacca caaaggcgcg
    49861 aagctggtca gcatcgtcaa caccgcgagc caccattccc tagcgttcgg tattaatttg
    49921 ccagcgtaaa tcctgacgct gggtttcgcc tgccagatgc agcggtaatt ccggcgtttt
    49981 gattttcacc agcatcgtcg gtagtttcag cggcgtgtta ttgccgagaa gattttttgc
    50041 cagcaccatc gcgctaagtt gaatcggctg caggaatggc agtacctgac cgttgatttc
    50101 cgcgtaatcg cccagcgcat aaatatcgtc attactggtt tgcagataac tatcgacgca
    50161 aacgccgcga ttaatcgtca gcccggcgcg tcgtgccagg gctgtttccg ggcgcagtcc
    50221 ggtggcggca attaccgcat ccacttcgat actacgctgg tggtccagcg ttgccagaat
    50281 gccagaatcc gttttttcca gcccctgtaa ctgagatttc aacagcagat gaacgcccat
    50341 ctccgtcaac cgatgctgca agcggctgct tacttccggt ggcattaacg acgccagaat
    50401 actggcagcg ttgtcgatta gcgtgaccgc tttgcctgca cgacaaaaat ccatcgccag
    50461 ttcgctacca atcaaaccac cgccaacaat caacacgcgt cgggcatccc gcagttgcgt
    50521 ttcacaggcg cgatactctt gctgactatt taacgtcagc attaactcac gcccaggctc
    50581 aggcgggaca aaggcactgg caccggttgc cagtactagc ttgtcgtatt gccactgatt
    50641 attctggctt ttcaccacac gggcttcggc atcgatatcc gtcacccagg tttgtggaaa
    50701 caggtgcaga ttaaactgct cggcaaattc acccgccgtc tggcgggtaa ggtcatcggc
    50761 acgttgcccc tgactgataa catggctgag gtcaggtttg ttgtactcat ccatgctgtc
    50821 ggcggcaatc agggttaatg gaatagtggc gtcctgtttg cgaatatttt tcaccagttg
    50881 gcgggcggcg aagcccgaac cgatgatcac aatgccgtta ctcattttgc ctccgatgcc
    50941 agttcttcaa agacgtcttt gccgagggag cattccgggc agaggaagtt atccgggact
    51001 tcactccacg gcgttcctgg cgcaacgtcc tgcattggct cgccttttgc cggatcgtaa
    51061 atccactggc agacgctgca ctgcatccgt gggccgaggt cagccgtcgt ggtggcagag
    51121 gtttcttctt tgactacctt attcaccgtg ctctgcggca gcggcgcgag cgcccactga
    51181 cgggcgattt cgcgaccgtg ttcacggcat aactccagag cgtcctggtc tggtcgccat
    51241 ttcgctttca ggctaagcga catttcgaaa cccgcatcct gcaggcgcgt ggaaagacga
    51301 tccaccgcac cgccgctcca gccgtgagag ccgaaagcac tggcgcgttt gttacggaag
    51361 cgtaaaccag tcatctcctc caccagcccg gcgattttcg gcatcatcac gttattcatc
    51421 gtcgaagtgc cgaccagcac gccttttgag cggaagacat tggtcaggat ttcgttttta
    51481 tcgcttcggg cgacgttgaa aattttcacc gccacgcgtg ggtcggtttc cgcaatcccc
    51541 tgggcgatag cgtcagccat catgcgggtg ttattcgaca tggtgtcgta gaaaatggtg
    51601 attctgtctt cctgataatc agccgcccat ttcaggtaca gctcgacaat ttgcgtcggg
    51661 ttatcgcgcc ataccacgcc gtgagaggtc gctatcatat cgactggtaa gttaaagccc
    51721 aggatctcgg taattttcgg tgttaccagg cggctgaacg gcgtcaggat attggcgtag
    51781 taacgctggc actgctcgaa aagctccgtc tgatccactt catcgttgaa cagatgctcg
    51841 tcgcagtagt gctgaccgaa agcatcgtta ctgaacagca ccgcgtcgcc tgtcaggtaa
    51901 gtcatcatgc tgtccggcca gtgcagcatt ggcgtttcga caaaaatgag ctgtttgccg
    51961 ttgccgatat ccagcgtgtc accagttttc accacattaa aattccactc cggatgatgg
    52021 tgatgaccat ttatcgagtc gatagcgttg gcagtacagt aaatcggcgt atcgggaatt
    52081 tgcgccatca gttcggtcag cgccccagcg tggtcctctt ctgcatggtt aatcacgatg
    52141 taatcgatat ccgccagatc gatttcatta cgcaggttct gcacaaattc gcggctgaat
    52201 ttatggtcga cggtgtcgat cagcacgttt ttttcttcgc ggatgaggta gctgttgtaa
    52261 ctgctgccgc gcagcgtttt atattccgta ccgtgaaaat cacgcacttc ccagtcacgt
    52321 tgaccaaccc aatgaatgtt atttttcacc acaatagaca tagcaacctc aatttattca
    52381 gcgtgttcta aaaagatgtc ttgctaattg cagattacgt gccagttttt tatctgattg
    52441 tttttactct ataaataaaa ttatgctcat taatgagtag tcaaaatgac tatcaatcaa
    52501 atggtcgata tgacaatatc tatagtcaaa ttgacagtga ggcaaagatg agtttttccg
    52561 ttgatgtgct ggcgaatatc gccatcgaat tgcagcgtgg gattggtcat caggatcgtt
    52621 ttcagcgcct gatcaccacg ctacgtcagg tgctggagtg cgatgcgtct gcgttgctac
    52681 gttacgattc gcggcagttt attccgcttg ccatcgacgg tctggcaaag ggtgtactcg
    52741 gtagacgctt tgcgctggaa gggcatccac ggctggaagc gattgcccgc gccggggatg
    52801 tggtgcgctt tcccgcagac agcgaattgc ccgatcccta tgacggtttg attcctgggc
    52861 aggagagtct gaaggttcac gcctgcgttg gtctgccatt gtttgccggg caaaacctga
    52921 tcggcgcact gacgctcgac gggatgcagc ccgatcagtt cgatgttttc agcgacgaag
    52981 agctacggct gattgctgcg ctggcggcgg gagcgttaag caatgcgttg ctgattgaac
    53041 aactggaaag ccagaatatg atgccaggcg atgccacgcc gtttgaagcg gtgaaacaga
    53101 cgcagatgat tggcttgtcc cctggcatga cgcaactgaa aaaagagatt gagattgtgg
    53161 cggcgtccga tctcaacgtc ctgatcagcg gtgagacggg aaccggtaag gagctggtgg
    53221 cgaaagcgat tcatgaagcc tcgccacggg cggtgaatcc gctggtctat ctcaactgtg
    53281 ctgcactgcc ggaaagtgtg gcggaaagtg agttgttcgg gcatgtgaaa ggagcgttta
    53341 ctggcgctat cagtaaccgc agcgggaagt ttgaaatggc ggataacggc actctgtttc
    53401 tggatgagat cggcgagttg tcgttggcat tgcaggccaa gctgctgagg gtgttgcagt
    53461 atggcgatat tcagcgcgtt ggcgatgacc gtagtttgcg ggtcgatgtg cgcgtgctgg
    53521 cggcgactaa ccgcgactta cgcgaagagg tgctggcagg gcgatttcgc gctgacttgt
    53581 ttcatcgcct gagcgtgttt ccactttcgg tgccgccgct gcgtgagcgg ggcgatgatg
    53641 tcattctgct ggcggggtat ttctgcgaac agtgtcgttt gcggctgggg ctctcccgcg
    53701 tggtattaag tgccggagcg cgaaatttac tgcaacacta tcgttttccg gggaacgtgc
    53761 gcgaactgga acatgctatt catcgggcgg tagtgctggc gagagccacc cgcaacggcg
    53821 atgaagtgat tcttgaggcg caacattttg cttttcctga ggtgacgttg ccgccgccag
    53881 aagcggcggc ggtgcccgtt gttaagcaaa acctgcgtga agcgacagaa gcgttccagc
    53941 gtgaaactat tcgccaggca ctggcacaaa atcatcataa ctgggctgcc tgcgcgcgga
    54001 tgctggaaac cgacgtcgcc aacctgcatc ggctggcgaa acgtctggga atgaaggatt
    54061 aaataatccc ggcctgatag aaatcctgca ggttaattgc gccggtgagt ttgccgtttt
    54121 catccaccac cggtgcggca gtgattttgc gcttcatcag gatctctttg gcgtcgatgg
    54181 cgcgactttg cgcctgtaac gtggtgccgc ctgtcgtcat cgcttcatta actggcgtgg
    54241 tgagtgcgcc gccgcctacc agccagcgac gtaaatcgcc gtcggtaaag acgccttgta
    54301 cctgttgttg agcgtcacat accgccacca gccccagacc ggtgcggctg agttccagca
    54361 tcgcatccat cacgctggcg gttaacgcca cctgtgggat ggcatcgtca cggcgcatca
    54421 gatgatgcac tttattcagc aagcgagcgc ccagtgcccc ggctgggtgg gagcgggcaa
    54481 aatcttcttc attaaatccg cgcgcctgca tgaccgccat cgccagcgcg tcacccatca
    54541 tcagggtatt gacggtgctg gaggtcggcg caaggtgcat cgggcaggct tcgcgttcta
    54601 cggagatatc cagcaccgct tttgccgcca ggcccagcgg tgacgtcggt ttgccggtca
    54661 tcgccagcag cgcgatagat ttatcttcca gacgcggaat aatcagatcc agttccttcg
    54721 cgccaccgga gtaagagata aacagcatca catcgcggct ttcaatcatc cccagatcgc
    54781 cgtgcagtgc ttctgccgga tggacaaaaa aagccggtgt gccggtactg gcaagtgttg
    54841 cggcgatttt cttaccaatg tggcccgatt tgccaattcc cgaaaccacc actttgcctt
    54901 cgcagtgcag gatgatattg gcggcgcgaa caaaatcatc gcccagacgt tccggtaaac
    54961 ggcttgcttc ctgcaactcc agcattaacg tctgacgtcc cgcgttcagt agtgcttcac
    55021 tcattgctct ctccggttat gatcacatcg atccctttct cttccagcgc ctgacgaaac
    55081 gccggatcga tacctgcgtc ggtaatcagc ttatcgacgc tttcaagact gcaaactacg
    55141 ttggggcttt tacggccaaa ctttgatgag tccgccatca aaatcacttc gcgcgcggca
    55201 ttgcacattg ccttactgac ggtataaacc tcgttgaagg tggttacgcc cgcattgaga
    55261 tcgatgccgt cggtgcccat aaacaattta tcgaaactga aatgttcgaa ggcattctct
    55321 gccagctgtc cgtgaaatga ggccgatttt ttacgaaacg ttccgcctgg catcaggata
    55381 gtttgttcgt tatccagttc ggatagcgca ttgacgatat gcaggctgtt ggtcatcacc
    55441 gtgatgttat taaagcgcga gagcagggga accatctgca aaacggtact gccagcatca
    55501 agaatgatcg aatcgccatc atggataaaa ctaacggcag cttctgcaat cagctctttc
    55561 ttgtgggtgt tgatgagtgt tttatgatcg ataggcggat cggattcctc tttattcaac
    55621 accactccgc cataagtacg aatgacggtt ccggcatgtt ccagaatgac cagatctttg
    55681 cgaatggttg tgcctgtggt gtcaaagtat tgcgccaatt cttcaaccga gcatttaccc
    55741 tgcttttgca gatactccag aatggcggcc tgacgctgac gaggtttcat gggcgtgatt
    55801 acctgattta ggtttcaaaa tgataacttc gcaagcaagt aacattcaca acgaaattat
    55861 ccacgtttca gtttaagcgc caatgataga gcattctgtg atagtggatc atggggaaag
    55921 atcacatcgg gctgtaaatc acccgcatgc ataccggtaa ttgcgggaat tttttgcggt
    55981 cgggcgaaga cggtcagtcc tttcataaac aaggtgtcga cgatgcgctg ctgatcgtcc
    56041 agccgatggc gaccacgacc cgcggtttaa agcgcccgct ggaacgaccc acgccaaccc
    56101 gcccttgctg gcatagtgtg tcgaaggcac ggttaaaacg agaaatttgc cagccgccta
    56161 aggccagttg cgcacaccag gcgataacgg cgacggtgat gagtgcggat accataactt
    56221 ttccttaatg tagtggcgga agtcctgtat gcctgatgag cgtagcgcat caggcattct
    56281 atagtctcac ggagatcctt aatggatctc cgctgttgat cagaacatca cctgaccgcc
    56341 ggtgacattg atcgactgtc cggtgcagta cgacgcctta ggactggcgt agaacagcag
    56401 catattcagc acatcttgat aatcgcagcc gcgtttgagc ggtactttgt caatgtaata
    56461 ctgctcgact tgatccggtt tgatacccag cttggtcgcg tattgtggca acagtgactg
    56521 gaacatcggc gatttcagca ggttaccgag catcagtgaa tgcaccgtaa tgccgtactc
    56581 cgccagatcc agcgccagtg attgagtcag cccgacgcca ccaaatttcg ctgcgctgta
    56641 gccagagttg tgtttgctgc ccactttgcc ggatttcgag ttgatctgaa taatgcgacc
    56701 ctgaatcccg tcgcggatca tcaaacgcga aaattcacgg gcacacagga aataacccac
    56761 cagattcact tgtagcgaac ggtcaaaatc gccgagctgg aagtcgctga taaaggctgc
    56821 ttttgctatt ccggcgctat agaccagcaa atccacgcga ccaaagattt catctacccc
    56881 acgagagagc gccagaacgc tttgctcgct agtggcgtca gcaccaaaac cgtacgccat
    56941 actttcacca tattcggcgt taatttcttg tgccacattt gcggctttgt cgctctgaat
    57001 atcgacaacc gcgacgcgat acccctcggc aaccagaccg tggcacagga acgcgcctaa
    57061 ggtttgccca ccaccgatga caacggcaac ctgattcatt ttttactcct taacagattc
    57121 aaacttcaaa accgatcccg gcgcgatatc gtcgggtaca gggcctgcca catggacagt
    57181 gcccggaaat tccgcttcgt ttaaaccatc gaagcgcagg gtgacatgac ccagttcgcg
    57241 aaggttgtct tccgccacgc tgccaacagc ggtcaccgga tagcgatgct gcccgagcgt
    57301 aaattgcaaa ccgggatgga gtgcaccttt caactcgccg tggcaatgaa tgaagcaata
    57361 ctcttcgagg tccgctggcg cgccttcacg aaaggtgatg agcatctggt cgctgagggc
    57421 gtcagtggca ctcgcgccga tacgggtgat ggtggtctta taaataacgg tcatgtttca
    57481 gcctctattg atagataaaa ccggagacaa accaggcgat cagtacagtc ggtgcgccgg
    57541 ttaaaaagcg gctcaccagt acagaaggga caccgacgcg aaccgtgtcc tgacgggctt
    57601 ccgccagcga caaaccgacc gggatgaagt cgcaggccgc ctgcgcgttg atggcaaaca
    57661 gtgccggtaa agccagatgc ggcggaatat tgccgagacc aatctgcacg ccaatcaata
    57721 cgccgataac ctgtgcgata actgcgcctg ggccgaggaa aggtgaaagc agtgggaagg
    57781 agcagatgag cgccagcatg accagaccca gtggatggct cgccagcgga gcaagaccgt
    57841 gggcaatcca gtcaccaagg ccagaagcca taatgatgcc aatgagcgcc gagacgaatg
    57901 ccataaacgg cagaatggtt tttaatacag tgtcgatggt gtcacgacca gattgaaaca
    57961 gcaccgcaac ggcggacccc atgcccattc ccaccttcgc cagtaaacca tcgctttgtt
    58021 cggtgatttt cttactggtg tcatagtcac ggcccacgga agagggttgt ggtgtcgcat
    58081 cacccactac agtgatgttt tcttctttta cgccagagac ataaatatct tccacaatgt
    58141 actgcgccag cggaccggac ttgcccgtcg agtggatatt aatggtggga atacgtcgtt
    58201 tcggatagat gccgcagcgt aatgtgccgc cacagtcgat taccgcgaca ccaatttccg
    58261 cctccgcggg ttcaccttct ttaaatccgt caatagcctg ccagccagta agttgtgcca
    58321 gtttgtcaac aatcgcaggc cgggtaccgg cggtgatata gacgattttt ttgcccggcg
    58381 tggcttccag ctcaagcggg ccgccccagc cacccgttcc tttttcgatc cgaatacgcg
    58441 tcatgatgtt gctcctgcca ggtgaacttt ttgttcaagt tgaatgccca tctttttctc
    58501 aaaaatggcg gtggtcagat cggtcaccca gccgcgaaag aagttggtga ccagaccaac
    58561 cagcagatag ctcaacgcca gtgggccaag tggcaggttc agcgttgtca gaccgctggc
    58621 aatgccaaga taaacaaaca gttcgccagg gttgatatgg ggaaagaggc cattcattga
    58681 gtggcagcta taagaggccg ccgcgtagta gctgggtttg tacttttccg gcataaagcg
    58741 accctggctt aaggtcatcg gattgcaaaa gacaaacgtg ccaatgcacg gtagcagcag
    58801 gtaacgggaa acagggttac cggcgcaacg ttgagcaaaa cgttcaatac gatgctgacc
    58861 gataaaatta atcagtgcgt tcatgataac cagcaggcta atcaacagcg gaagaatgcc
    58921 ggtcaccatc ccggtaaaca cctctccgcc cttttggaac agcccgataa accactctgc
    58981 accatgagta atggtttcta tcattgttct ctccttcagg atttattgtt ttattaccaa
    59041 acggcaacct aatctaatca gattgaaaga tttaaaagtg ttattttaat ctaaaaatga
    59101 aagataaata ttttaatttg gaagtttgag taaaaggata gcgaggggaa tgagttgagt
    59161 tatgtaaagt ccgtatcggg cagtgactac cgcttccttg tgcggggcgt gatgctttac
    59221 catacttgcc cctggttgaa tctgttaaat ggacccctca tgttcaagcg tcgttatgta
    59281 acattgcttc ccctttttgt gttgcttgcc gcctgtagca gcaagccaaa acctactgag
    59341 actgaaacga ccaccggaac gccgtctggc ggcttcctgc ttgagccgca gcacaatgtg
    59401 atgcagatgg gcggcgattt cgctaataac ccgaatgccc agcagttcat cgacaaaatg
    59461 gtgaacaaac acggtttcga tcgtcagcag ttgcaggaaa ttctctccca ggcgaagcgt
    59521 ctggattcgg tactgcggct gatggataac caggcaccaa ccacatcggt gaaaccgcca
    59581 tcaggtccga acggcgcatg gctgcgttat cgcaaaaaat ttattacgcc ggacaacgtg
    59641 cagaacggtg tggttttctg gaatcagtat gaagatgcgt tgaatcgcgc gtggcaggtg
    59701 tatggcgtac cgccggaaat tatcgtcggg attatcggcg ttgaaacccg ctgggggcgc
    59761 gtgatgggga aaactcgcat cctcgatgcg ctggcaacgc tgtcatttaa ctacccacgc
    59821 cgcgcggagt atttctctgg cgagctggaa accttcctgc tgatggcgcg cgacgagcag
    59881 gacgatccgc tcaatctgaa aggttccttt gccggggcga tgggctacgg acagtttatg
    59941 ccgtcgtctt acaaacaata tgcggtagat ttcagcggcg acgggcatat caacctgtgg
    60001 gatccggttg atgcgatcgg tagcgtggcg aactatttca aagcgcacgg ctgggtgaaa
    60061 ggcgatcagg tcgcggtaat ggcaaacggt caggctccag gcttgccaaa tggcttcaaa
    60121 accaggtaca gcatttcgca gcttgccacg gcaggtttaa cgccacagca gccgctgggc
    60181 aaccatcaac aagccagcct gctgcgtctg gatgtcggta ccggttacca gtactggtac
    60241 ggtctgccga acttctacac cattacccgt tataaccaca gcacccatta cgcaatggcg
    60301 gtctggcagt taggacaagc cgtggcgctg gcgcgcgtgc agtaactatt tgcaagaggg
    60361 gggcgtgagc ttccctctgg ctaatcagct tttttctgaa tcctcctcgt aaaattgcaa
    60421 cgccaacacc atcttcctga cgaaagtgct atcttgtccg gcataaattt ttgactgaca
    60481 gaggttgtga tgactgacag tgaactgatg cagttaagtg aacaggttgg gcaggcgctg
    60541 aaagcccgtg gcgcaaccgt aacaactgcc gagtcttgta ccggtggttg ggtagcgaaa
    60601 gtgattaccg atattgccgg tagctccgcc tggtttgaac gcggatttgt cacctacagt
    60661 aacgaagcca aagcgcagat gatcggcgta cgcgaagaga cgctggcgca gcatggcgcg
    60721 gtgagtgaac ccgtcgtggt ggaaatggtg ataggcgcac tgaaagcggc tcgtgctgat
    60781 tatgccgtgt ctattagtgg tatcgccggg ccggatggcg gcagtgaaga gaagcctgtc
    60841 ggcaccgtct ggtttgcttt tgccactgcc cgcggtgaag gcattacccg gcgggaatgc
    60901 ttcagcggcg accgtgatgc ggtgcgtcgt caggctactg cgtatgcatt gcagaccttg
    60961 tggcaacaat ttctacaaaa cacttgatac tgtatgagca tacagtataa ttgcttcaac
    61021 agaacatatt gactatccgg tattacccgg catgacagga gtaaaaatgg ctatcgacga
    61081 aaacaaacag aaagcgttgg cggcagcact gggccagatt gagaaacaat ttggtaaagg
    61141 ctccatcatg cgcctgggtg aagaccgttc catggatgtg gaaaccatct ctaccggttc
    61201 gctttcactg gatatcgcgc ttggggcagg tggtctgccg atgggccgta tcgtcgaaat
    61261 ctacggaccg gaatcttccg gtaaaaccac gctgacgctg caggtgatcg ccgcagcgca
    61321 gcgtgaaggt aaaacctgtg cgtttatcga tgctgaacac gcgctggacc caatctacgc
    61381 acgtaaactg ggcgtcgata tcgacaacct gctgtgctcc cagccggaca ccggcgagca
    61441 ggcactggaa atctgtgacg ccctggcgcg ttctggtgca gtagacgtta tcgtcgttga
    61501 ctccgtggcg gcactgacgc cgaaagcgga aatcgaaggc gaaatcggcg actctcacat
    61561 gggccttgcg gcacgtatga tgagccaggc gatgcgtaag ctggcgggta acctgaagca
    61621 gtccaacacg ctgctgatct tcatcaacca gatccgtatg aaaattggtg tgatgttcgg
    61681 taacccggaa accactaccg gtggtaacgc gctgaaattc tacgcctctg ttcgtctcga
    61741 catccgtcgt atcggcgcgg tgaaagaggg cgaaaacgtg gtgggtagcg aaacccgcgt
    61801 gaaagtggtg aagaacaaaa tcgctgcacc gtttaaacag gctgaatttc agatcctcta
    61861 cggcgaaggt atcaacttct acggcgaact ggttgacctg ggcgtaaaag agaagctgat
    61921 cgagaaagca ggcgcgtggt acagctacaa aggtgagaag atcggtcagg gtaaagcgaa
    61981 tgcaactgcc tggctgaaag ataacccgga aaccgcgaaa gagatcgaga agaaagtacg
    62041 tgagttgctg ctgagcaacc cgaactcaac gccggatttc tctgtagatg acagcgaagg
    62101 cgtagcagaa actaacgaag atttttaatc gttcgttatt gatgataata gataatgtgt
    62161 gcacggacgg tcccctcgcc cctttgggga gagggttagg gtgaggggaa caggccagca
    62221 ctggcgtgaa aaattactct cacctcgatc ctctaacgaa ttgggcctgc gggaacattc
    62281 tgcacactca ttgactatta aatctgttta tcagcaaaac ctgggtcgca tctgcggccc
    62341 ttttgctttt ttaagttgta aggatatgcc atgacagaat caacgtcccg tcgcccggca
    62401 tatgctcgcc tgttggatcg tgcggtacgc attctggcgg tgcgcgatca cagtgagcaa
    62461 gaactgcgac gtaaactcgc ggcaccgatt atgggcaaaa atggcccaga agagattgat
    62521 gctacggcag aagattacga gcgcgttatt gcatggtgcc atgaacatgg ctatctcgat
    62581 gacagccgat ttgttgcgcg ctttatcgcc agccgtagcc gcaaaggtta tggacctgca
    62641 cgtattcgtc aggaactgaa tcagaaaggt atttcccgcg aagcgacaga aaaagcgatg
    62701 cgtgaatgtg acatcgactg gtgcgcactg gcgcgcgatc aggcgacgcg aaaatatgat
    62761 gaacctttgc caactgtctt ttcagaaaaa gttaagatcc agcgttttct gctctatcgt
    62821 ggctatctga tggaagatat ccaggatatt tggcgaaatt ttgccgactg aacgcatacg
    62881 gtattttact tcccagtcaa gaaaacttat cttattccca cttttcagtt accagcccgg
    62941 cggttaagac acgctggagc tggtggcgat atttcgttag cttgatttca ggataattat
    63001 gagcaagagc accgctgaga tccgtcaggc gtttctcgac tttttccata gtaagggaca
    63061 tcaggtagtt gccagcagct ccctggtacc ccataacgac ccaactttgt tgtttaccaa
    63121 cgccgggatg aatcagttca aggatgtgtt ccttgggctc gacaagcgta attattcccg
    63181 cgctaccact tcccaacgct gcgtgcgtgc gggtggtaaa cacaacgacc tggaaaacgt
    63241 cggttacacc gcgcgtcacc ataccttctt cgaaatgctg ggcaacttca gcttcggcga
    63301 ctatttcaaa cacgatgcca ttcagtttgc atgggaactg ctgaccagcg aaaaatggtt
    63361 tgccctgccg aaagagcgtc tgtgggttac cgtctatgaa agcgacgacg aagcctacga
    63421 aatctgggaa aaagaagtcg ggatcccgcg cgaacgtatt attcgcatcg gcgataacaa
    63481 aggtgcgcca tacgcatctg acaacttctg gcagatgggt gacactggtc cgtgcggccc
    63541 gtgcaccgaa atcttctacg atcacggcga ccacatttgg ggtggccctc cgggaagtcc
    63601 ggaagaagac ggcgaccgct acattgagat ctggaacatc gtcttcatgc agttcaaccg
    63661 ccaggcagat ggcacgatgg aaccgctgcc gaagccgtct gtagataccg gtatgggtct
    63721 ggagcgtatt gctgcggtgc tgcaacacgt taactctaac tatgacatcg acctgttccg
    63781 cacgttgatc caggcggtag cgaaagtcac tggcgcgacc gatctgagca ataaatcgct
    63841 gcgcgtaatc gctgaccaca ttcgttcttg tgcgttcctg atcgcggatg gcgtaatgcc
    63901 gtccaatgaa aaccgtggtt atgtactgcg tcgtatcatt cgtcgcgcag tgcgtcacgg
    63961 caatatgctc ggcgcgaaag aaaccttctt ctacaaactg gttggtccgc tgatcgacgt
    64021 tatgggctct gcgggtgaag acctgaaacg ccagcaggcg caggttgagc aggtgctgaa
    64081 gactgaagaa gagcagtttg cccgtactct ggagcgcggt ctggcgttgc tggatgaaga
    64141 gctggcaaaa ctttctggtg atacgctgga tggtgaaacg gctttccgtc tgtacgacac
    64201 ctatggcttc ccggttgacc tgacggctga tgtttgtcgt gagcgcaaca tcaaagttga
    64261 cgaagctggt tttgaagcag caatggaaga gcagcgtcgt cgcgcgcgcg aagccagcgg
    64321 ctttggtgcc gattacaacg caatgatccg tgttgatagt gcatctgaat ttaaaggcta
    64381 tgaccatctg gaactgaacg gcaaagtgac cgcgctgttt gttgatggta aagcggttga
    64441 tgccatcaat gcaggccagg aagctgtggt cgtgctagat caaacaccat tctatgcgga
    64501 atccggcggt caggttggtg ataaaggcga actgaaagac gctaacttct ccttcgcggt
    64561 ggaagatacg cagaaatacg gccaggcgat tggtcacatc ggtaaacttg ctgcgggttc
    64621 tctgaaagtg ggcgacgctg tgcaggctga tgttgatgag gctcgtcgcg cccgtattcg
    64681 tctgaatcac tccgcaacgc acctgatgca cgctgcgctg cgccaggttc tggggactca
    64741 tgtatcgcag aaaggttcac tggttaacga caaggtgctg cgcttcgact tctcacacaa
    64801 cgaagcgatg aaaccggaag agattcgtgc ggtcgaagac ctggtgaacg cacagattcg
    64861 tcgcaatttg ccgatcgaaa ccaacatcat ggatctcgaa gtggcgaaag cgaaaggtgc
    64921 gatggcgctg ttcggcgaga agtatgatga gcgtgtacgc gtgctgagca tgggcgattt
    64981 ctccaccgag ctgtgtggcg gtactcacgc cagccgcact ggtgatattg gtctgttccg
    65041 catcatctct gaatcgggta ctgctgcagg cgttcgtcgt atcgaagcgg taaccggaga
    65101 aggcgctatc accaccgttc atgcagacag cgatcgctta agcgaagtcg cgcatctgct
    65161 gaaaggcgat agcaataatc tggcggataa agtgcgctca gtactggaac gtacgcgtca
    65221 gttagaaaaa gaactacaac agcttaaaga acaagctgcc gcacaggaga gcgcaaatct
    65281 ttccagtaag gcaattgatg ttaatggtgt taagctgttg gttagcgagc ttagcggtgt
    65341 tgagccgaaa atgttgcgta ccatggttga cgatttaaaa aatcagctgg ggtcgacaat
    65401 tatcgtgctg gcaacggtag ccgaaggtaa ggtttctctg attgcaggcg tatctaagga
    65461 cgtcacagat cgtgtgaaag caggggaact gattggtatg gtcgctcagc aggtgggcgg
    65521 caagggtggt ggacgtcctg acatggcgca agccggtggt acggatgctg cggccttacc
    65581 tgcagcgtta gccagtgtga aaggctgggt cagcgcgaaa ttgcaataat ataagcgtca
    65641 ggcaatgccg tggactcgct tcacggcatt cgcattaacg ctatcgacaa cgataaagtc
    65701 aggttgaagt tgtgtatatc ggctaaactt aggtttaaca gaatgtaatg ccatgactgc
    65761 ttacatgtaa tgtgtttgtc attgcttact ttttggcgtt atatgatgga taatgccggg
    65821 atacagagag acccgactct tttaatcttt caaggagcaa agaatgctga ttctgactcg
    65881 tcgagttggt gagaccctca tgattgggga tgaggtcacc gtgacagttt taggggtgaa
    65941 gggcaaccag gtacgtattg gcgtaaatgc cccgaaggaa gtttctgttc accgtgaaga
    66001 gatctaccag cgtatccagg ctgaaaaatc ccagcagtcc agttactaat ctttccgcgt
    66061 ctcatcttta tcggtgagac gcaccctcaa aatttctcct tcactctata gtcttttcgc
    66121 tttactcccg ttcattcaac ttaagtctcc atttttttgc attactgcta tctgccagac
    66181 ctccgttttt ctgtagataa aacactcttt ttgacgtttt tacagactaa ttgagcgtga
    66241 agtgtgcaaa cgataaaagt gtgggaaaaa ttgtttgact tataagtctc agaaagtaat
    66301 atgtgcgcca cgcagcgacg atgagcgata aacaagttct tcgaagcact cgtaagaggc
    66361 gtgtggtgag gtggccgaga ggctgaaggc gctcccctgc taagggagta tgcggtcaaa
    66421 agctgcatcc ggggttcgaa tccccgcctc accgccattt gcatccgtag ctcagctgga
    66481 tagagtactc ggctacgaac cgagcggtcg gaggttcgaa tcctcccgga tgcaccatat
    66541 tctacgtact ttcagcgatg aaggtatgga agaggtggcg gtataaccgc aggcaccagg
    66601 gaggataacg ttgctttagc aacggccaga agggcgagcc gcaaggcgag taatcctccc
    66661 gaatgcacca tctcttactt gatacggctt tagtagcggt atcaaaaaat ctgcagtaaa
    66721 gtaa
//
LOCUS       DAJTYD010000004        65402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTYD010000004 DAJTYD010000000
VERSION     DAJTYD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.004"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1271)
                     /gene="pcnB"
                     /locus_tag="OO585_000238"
     CDS             complement(<1..1271)
                     /gene="pcnB"
                     /locus_tag="OO585_000238"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6627946.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAEL"
     gene            complement(1310..2236)
                     /gene="gluQRS"
                     /locus_tag="OO585_000239"
     CDS             complement(1310..2236)
                     /gene="gluQRS"
                     /locus_tag="OO585_000239"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6627947.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2273..2728)
                     /gene="dksA"
                     /locus_tag="OO585_000240"
     CDS             complement(2273..2728)
                     /gene="dksA"
                     /locus_tag="OO585_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6627948.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(2906..3610)
                     /gene="sfsA"
                     /locus_tag="OO585_000241"
     CDS             complement(2906..3610)
                     /gene="sfsA"
                     /locus_tag="OO585_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6627949.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(3625..4155)
                     /gene="thpR"
                     /locus_tag="OO585_000242"
     CDS             complement(3625..4155)
                     /gene="thpR"
                     /locus_tag="OO585_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6627950.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4229..6658
                     /gene="hrpB"
                     /locus_tag="OO585_000243"
     CDS             4229..6658
                     /gene="hrpB"
                     /locus_tag="OO585_000243"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6627951.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            6854..9376
                     /gene="mrcB"
                     /locus_tag="OO585_000244"
     CDS             6854..9376
                     /gene="mrcB"
                     /locus_tag="OO585_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6627952.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            9596..11785
                     /gene="fhuA"
                     /locus_tag="OO585_000245"
     CDS             9596..11785
                     /gene="fhuA"
                     /locus_tag="OO585_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6627953.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            11836..12633
                     /gene="fhuC"
                     /locus_tag="OO585_000246"
     CDS             11836..12633
                     /gene="fhuC"
                     /locus_tag="OO585_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6627954.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            12633..13523
                     /gene="fhuD"
                     /locus_tag="OO585_000247"
     CDS             12633..13523
                     /gene="fhuD"
                     /locus_tag="OO585_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6627955.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            13520..15502
                     /gene="fhuB"
                     /locus_tag="OO585_000248"
     CDS             13520..15502
                     /gene="fhuB"
                     /locus_tag="OO585_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6627956.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(15537..16817)
                     /gene="hemL"
                     /locus_tag="OO585_000249"
     CDS             complement(15537..16817)
                     /gene="hemL"
                     /locus_tag="OO585_000249"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6627957.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17042..18463
                     /gene="clcA"
                     /locus_tag="OO585_000250"
     CDS             17042..18463
                     /gene="clcA"
                     /locus_tag="OO585_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6627958.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            18487..18552
                     /locus_tag="OO585_000251"
     CDS             18487..18552
                     /locus_tag="OO585_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6627959.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            18545..18889
                     /gene="erpA"
                     /locus_tag="OO585_000252"
     CDS             18545..18889
                     /gene="erpA"
                     /locus_tag="OO585_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6627960.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(18936..19559)
                     /locus_tag="OO585_000253"
     CDS             complement(18936..19559)
                     /locus_tag="OO585_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6627961.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(19597..20396)
                     /gene="btuF"
                     /locus_tag="OO585_000254"
                     /pseudo
     CDS             complement(19597..20396)
                     /gene="btuF"
                     /locus_tag="OO585_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(20389..21087)
                     /gene="mtnN"
                     /locus_tag="OO585_000255"
     CDS             complement(20389..21087)
                     /gene="mtnN"
                     /locus_tag="OO585_000255"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6627962.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFEADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21171..22688
                     /gene="dgt"
                     /locus_tag="OO585_000256"
     CDS             21171..22688
                     /gene="dgt"
                     /locus_tag="OO585_000256"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6627963.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            22818..24242
                     /gene="degP"
                     /locus_tag="OO585_000257"
     CDS             22818..24242
                     /gene="degP"
                     /locus_tag="OO585_000257"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6627964.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            24397..25554
                     /gene="cdaR"
                     /locus_tag="OO585_000258"
     CDS             24397..25554
                     /gene="cdaR"
                     /locus_tag="OO585_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6627965.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(25643..26029)
                     /locus_tag="OO585_000259"
     CDS             complement(25643..26029)
                     /locus_tag="OO585_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6627966.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26191..27003)
                     /gene="yaeI"
                     /locus_tag="OO585_000260"
     CDS             complement(26191..27003)
                     /gene="yaeI"
                     /locus_tag="OO585_000260"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6627967.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27058..27882)
                     /gene="dapD"
                     /locus_tag="OO585_000261"
     CDS             complement(27058..27882)
                     /gene="dapD"
                     /locus_tag="OO585_000261"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6627968.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27913..30585)
                     /gene="glnD"
                     /locus_tag="OO585_000262"
     CDS             complement(27913..30585)
                     /gene="glnD"
                     /locus_tag="OO585_000262"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6627969.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30647..31441)
                     /gene="map"
                     /locus_tag="OO585_000263"
     CDS             complement(30647..31441)
                     /gene="map"
                     /locus_tag="OO585_000263"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6627970.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31809..32534
                     /gene="rpsB"
                     /locus_tag="OO585_000264"
     CDS             31809..32534
                     /gene="rpsB"
                     /locus_tag="OO585_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6627971.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32792..33643
                     /gene="tsf"
                     /locus_tag="OO585_000265"
     CDS             32792..33643
                     /gene="tsf"
                     /locus_tag="OO585_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6627972.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33790..34515
                     /gene="pyrH"
                     /locus_tag="OO585_000266"
     CDS             33790..34515
                     /gene="pyrH"
                     /locus_tag="OO585_000266"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6627973.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34807..35364
                     /gene="frr"
                     /locus_tag="OO585_000267"
     CDS             34807..35364
                     /gene="frr"
                     /locus_tag="OO585_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6627974.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35456..36652
                     /gene="ispC"
                     /locus_tag="OO585_000268"
     CDS             35456..36652
                     /gene="ispC"
                     /locus_tag="OO585_000268"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6627975.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36840..37598
                     /gene="ispU"
                     /locus_tag="OO585_000269"
                     /gene_synonym="uppS"
     CDS             36840..37598
                     /gene="ispU"
                     /locus_tag="OO585_000269"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6627976.1"
                     /translation="MLSATQLLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37611..38468
                     /gene="cdsA"
                     /locus_tag="OO585_000270"
     CDS             37611..38468
                     /gene="cdsA"
                     /locus_tag="OO585_000270"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6627977.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38480..39832
                     /gene="rseP"
                     /locus_tag="OO585_000271"
     CDS             38480..39832
                     /gene="rseP"
                     /locus_tag="OO585_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6627978.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39862..42294
                     /gene="bamA"
                     /locus_tag="OO585_000272"
     CDS             39862..42294
                     /gene="bamA"
                     /locus_tag="OO585_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6627979.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42416..42901
                     /gene="skp"
                     /locus_tag="OO585_000273"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42416..42901
                     /gene="skp"
                     /locus_tag="OO585_000273"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6627980.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42905..43930
                     /gene="lpxD"
                     /locus_tag="OO585_000274"
     CDS             42905..43930
                     /gene="lpxD"
                     /locus_tag="OO585_000274"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6627981.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44035..44490
                     /gene="fabZ"
                     /locus_tag="OO585_000275"
     CDS             44035..44490
                     /gene="fabZ"
                     /locus_tag="OO585_000275"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6627982.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44494..45282
                     /gene="lpxA"
                     /locus_tag="OO585_000276"
     CDS             44494..45282
                     /gene="lpxA"
                     /locus_tag="OO585_000276"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6627983.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            45282..46430
                     /gene="lpxB"
                     /locus_tag="OO585_000277"
     CDS             45282..46430
                     /gene="lpxB"
                     /locus_tag="OO585_000277"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6627984.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVARDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46427..47023
                     /gene="rnhB"
                     /locus_tag="OO585_000278"
     CDS             46427..47023
                     /gene="rnhB"
                     /locus_tag="OO585_000278"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6627985.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47060..50542
                     /gene="dnaE"
                     /locus_tag="OO585_000279"
     CDS             47060..50542
                     /gene="dnaE"
                     /locus_tag="OO585_000279"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6627986.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50555..51514
                     /gene="accA"
                     /locus_tag="OO585_000280"
     CDS             50555..51514
                     /gene="accA"
                     /locus_tag="OO585_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6627987.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51613..53722
                     /gene="ldcC"
                     /locus_tag="OO585_000281"
                     /pseudo
     CDS             51613..53722
                     /gene="ldcC"
                     /locus_tag="OO585_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            53779..54168
                     /locus_tag="OO585_000282"
     CDS             53779..54168
                     /locus_tag="OO585_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6627988.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54233..55531
                     /gene="tilS"
                     /locus_tag="OO585_000283"
     CDS             54233..55531
                     /gene="tilS"
                     /locus_tag="OO585_000283"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6627989.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55580..55840)
                     /gene="rof"
                     /locus_tag="OO585_000284"
     CDS             complement(55580..55840)
                     /gene="rof"
                     /locus_tag="OO585_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6627990.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55827..56027)
                     /locus_tag="OO585_000285"
     CDS             complement(55827..56027)
                     /locus_tag="OO585_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6627991.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56193..56738
                     /locus_tag="OO585_000286"
     CDS             56193..56738
                     /locus_tag="OO585_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6627992.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56735..57157
                     /gene="arfB"
                     /locus_tag="OO585_000287"
     CDS             56735..57157
                     /gene="arfB"
                     /locus_tag="OO585_000287"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6627993.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57171..57881
                     /gene="nlpE"
                     /locus_tag="OO585_000288"
                     /gene_synonym="cutF"
     CDS             57171..57881
                     /gene="nlpE"
                     /locus_tag="OO585_000288"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6627994.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58081..58901)
                     /locus_tag="OO585_000289"
                     /pseudo
     CDS             complement(58081..58901)
                     /locus_tag="OO585_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58954..60672)
                     /gene="proS"
                     /locus_tag="OO585_000290"
     CDS             complement(58954..60672)
                     /gene="proS"
                     /locus_tag="OO585_000290"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6627995.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60783..61490)
                     /gene="tsaA"
                     /locus_tag="OO585_000291"
     CDS             complement(60783..61490)
                     /gene="tsaA"
                     /locus_tag="OO585_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6627996.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61487..61891)
                     /gene="rcsF"
                     /locus_tag="OO585_000292"
     CDS             complement(61487..61891)
                     /gene="rcsF"
                     /locus_tag="OO585_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6627997.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62009..62824)
                     /gene="metQ"
                     /locus_tag="OO585_000293"
     CDS             complement(62009..62824)
                     /gene="metQ"
                     /locus_tag="OO585_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6627998.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62864..63517)
                     /gene="metI"
                     /locus_tag="OO585_000294"
     CDS             complement(62864..63517)
                     /gene="metI"
                     /locus_tag="OO585_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6627999.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63510..64541)
                     /gene="metN"
                     /locus_tag="OO585_000295"
     CDS             complement(63510..64541)
                     /gene="metN"
                     /locus_tag="OO585_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6628000.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64729..65301
                     /gene="gmhB"
                     /locus_tag="OO585_000296"
     CDS             64729..65301
                     /gene="gmhB"
                     /locus_tag="OO585_000296"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6628001.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15507 a        16216 c        17977 g        15702 t
ORIGIN      
        1 tgcagttcag cgttacgttc aacttcagct cgcaaggcca acaggtcata agccgcacgg
       61 aacttaggat gctccagcag tttccatgcg cgtttaccct gacgacggga catacgcaac
      121 tgcaactgcc agatatcgcg ggttaacgtc gtcagacgtt tcgggattgc cagtgaacgg
      181 caggcttcgt ccagcacgtc gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg
      241 ctttcctggg cgatcttctg tgccgtctcc agcagtgggt accagaacat ggcggcaaac
      301 aagaacgccg ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagcacc
      361 tgttcaatga tccgctccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc
      421 gggaacagcg gctggaacag atgatattca cacaacagct tataggtttc gtaaccgtag
      481 cccgcttgta gcagtttaag cgattcttca aacaggcgtg ccggtgggat atcgttcagc
      541 agggtagcga ggcgagggat cggttctgcg gtttccgggc tgatgcgcat acccaatttg
      601 gcggcaaaac gtaccgcgcg cagcatacgt accggatctt cacggtagcg cgtttccggg
      661 ttaccaatca gacggataac gccgtccttc agatccttca tgccgccaac gtaatcacgg
      721 acggtaaaat ccgctacgct gtaatacagg ctgttgatag tgaaatcgcg gcgctgggcg
      781 tcttcttcga tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg
      841 gaggtcgtgc ggtcgctgac gttaccttcg tggtgtccac ggaaggttgc aacttcgata
      901 atctccgggc caaacatcac atgagccaga cggaaacggc gacccacaag gcggcagtta
      961 cggaacagtt tgcgcacctg ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc
     1021 tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct
     1081 ttattgagcc tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca
     1141 tgctgctcac gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt
     1201 tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc
     1261 ggtagtgtca aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg
     1321 catttgagaa tgttgaattt acaattgccg actcgggcac ggcggtcagc gtccagtttt
     1381 tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgct tctacctgct
     1441 gccccagaaa ttgaagtgcc gcgattagta ccgggcgtgg atcgcctttc ggcaacgcag
     1501 gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggcagatgaa
     1561 tgtaatctgg cactttccag ccaaaaagct ggtacagcga gatttgcctt actgttggtt
     1621 caatcagatc agccccacgc actatttctg taacgccctg gaaatgatca tcaaccacaa
     1681 cagccaggtt gtaggcgaac aacccatcac ggcgatgaat gataaaatct tcccgtgcca
     1741 gtttttcgtc ggcgtgaata atgccgcgca gctggtcagt aaattgcgtg accggatgct
     1801 gctggcggat acgcactgcg gcgttgtctg gtccatgatg caacacccgg caatgaccgt
     1861 cgtaaatacc gacaatgctt tgaatacgcg cacgcgtaca ggtgcagtaa taacttagtc
     1921 cttgttcatg taaccaggcg agtgcttcac gataggcgtc gtgacgttgc gattgccaga
     1981 gaacatcgcc gtcccagtgc agaccgtaat gttccagctg gcgcaggata gtttctgcgg
     2041 caccgggaac ttcacgaggc gggtcgatat cttctatgcg taccagccag cgaccttgcc
     2101 gggcgcgagc ctgcaaatag ctgccgagcg cggcgatcag agagccaaaa tgaagctcgc
     2161 cggaaggaga gggggcgaag cggccaatat actgtgtgtc tgtcatctct ttgaacaaaa
     2221 aataaggcgg gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag
     2281 ccatctgttt ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg
     2341 ggcgcgcttc cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat
     2401 cttcgtcttc cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat
     2461 cgcggttacg cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg
     2521 ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt
     2581 gattacgcca tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact
     2641 cttcgcccgg cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg
     2701 atgttttacg gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc
     2761 catgttcggg ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta
     2821 tctaaacgta acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat
     2881 gtaaattaac cagttactta ctttactaca atgtaaccgg cagtgatttt ttaagagcca
     2941 tgccttcagc agaaatttcc gctttgtaag ccagaatttc tacccccctc tgttgagctt
     3001 ctgacaatag ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg
     3061 ctgaatgcag cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgttcatca
     3121 actcccgaag gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt
     3181 tctccgctaa cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca
     3241 acataaagtc aatacggctg cgttctgcgc catattttac ttcgcttttc agcgagctat
     3301 agcctgacag ttctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc
     3361 aaagcgtatt gacgcaaata aatgcgccgc tctggctttg agttagttcc caggtgtgtg
     3421 ggtatttccg tttggtgttg tctgaagtcg aataccagac ggtatcgcca cgcgttgcac
     3481 aaccggtcat cgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga
     3541 tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agaaggggag
     3601 agaattccat tgcgacaatc cttgttattg cattagcgcc cagcgtttta gcggcgtgta
     3661 gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgcataaga
     3721 ccagttaaaa cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat
     3781 atgtggatga aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag
     3841 catattcgcc agctggatta agccgcgtgg cggctgacgc atccctaacc acaccacacg
     3901 cgaacgcagc cattgtccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg
     3961 tccggctaaa agagaaagcg ccttctcttt ctcggcgctc acttcgccta aaaatgccag
     4021 agtcagatgc aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc
     4081 gcgccagtgg ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagacg
     4141 ttgcggttca gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc
     4201 gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgttttacc
     4261 tgaattactt accgctctcg attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc
     4321 cgggaaatca acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat
     4381 tatcctgctg gagccgcgtc gtctggcggc gcgtaacgtc gcgcaacggc tggaggagct
     4441 gcttaacgaa aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg
     4501 gccgaatacc cgcctggaag tagttaccga aggcgtgctg acgcggatga tccagcgtga
     4561 cccggagttg agcggtgttg gattggtgat ccttgatgaa tttcatgagc gtagcttgca
     4621 ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa
     4681 actgctaatt atgtcggcta cgctggacaa cgaccgcttg cagcaaatgc tgccagaagc
     4741 gcccgtcgtc atctcagaag ggcgctcgtt tccggttgaa cgccgttatt taccgctgcc
     4801 cgcgcatcag cgttttgacg atgccgttgc ggtagccacc gctgaaatgc tgcgtcagga
     4861 aagcggatca ttactgttat ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca
     4921 actggcttcg cgcatcggca gtgatgtatt gctctgcccg ctgtatggcg cgttgtcgct
     4981 gaacgatcag cgaaaagcga tcctcccggc accgcaaggg atgcgcaaag tggtgctggc
     5041 gaccaatatt gctgaaacca gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc
     5101 ccaggagcgt gtggcgcgtt ttgatccgcg cacggggcta acgcgactga ttactcaacg
     5161 cgttagccag gcatccatga cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat
     5221 ctgcctgcat ctaatcgcca aagaacaagc agaacgcgct gccgcgcaaa gtgaaccgga
     5281 aatcttacaa agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga
     5341 tccggcgcag atgagctggc tggatcaacc gccaacggtg aatctactgg ccgcgaaacg
     5401 cctgttacaa atgctggggg cgctggaggg tgaacggctt agtgcgcagg ggcaaaaaat
     5461 ggcagcgctg ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga
     5521 cgaagctgct accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa
     5581 tagtgacctg ggcgtggcgt tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca
     5641 actgttaaaa cgcttaaacg tacgtggcgg tgaggcagac agttcgctta tcgcgccgct
     5701 acttgccggg gcgtttgccg atcgcattgc tcgtcgccgt gggcaagatg gacgctatca
     5761 actggcaaac ggcatgggag cgatgctcga tgccgacgac gcgctaagcc gccacgaatg
     5821 gttgatcgca ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc
     5881 gctgccggtc gatattgatg agttagtaca acgctgcccg cagctggtac agcagtctga
     5941 cactgtggag tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaatcgg
     6001 tctgctgacg gtgaaagtgc agccgctggc gaaaccctcg gaagacgagt tgcatcaggc
     6061 gatgcttaat ggcatccgtg ataaaggttt aagcgtgctc aactggacgg cggaagcgga
     6121 acagctacgc ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc
     6181 ggttgatgat gaaagtttat tggcgacgct ggaaacgtgg ctgctgccac atatgactgg
     6241 cgtacattca ctacgcggcc tgaaatcact cgacatttat caggcactac gcggattact
     6301 tgattgggga atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac
     6361 gggaagccgg atcgccattc gttatcatga agataacccg cccgcgctgg cggtgagaat
     6421 gcaagagatg tttggcgagg ccaccaatcc gacgatcgcc caggggcgcg tgccgctggt
     6481 gctggagttg ctttcacctg cccaaagtcc attacaaatc acacgagatt tgagcgcctt
     6541 ctggaaagga gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccaaacatgt
     6601 ctggccggac gacccggcaa atactgcacc gacgcgacgg acgaaaaagt attcgtaagg
     6661 ttggttttct ccctctccct gtgggagagg gccggggtga gggcatcagc gcgcacgttc
     6721 accctcaccc taaccctctc cctcaaggga gaggggaccg atcgaacaca aattttgaga
     6781 gatatcttct tctgtcttgt aacagaagaa cagaaaatcg ggcttttgcg cctgaatatt
     6841 gcggagaaaa agcatggccg ggaatgaccg cgagccaatt ggacgcaaag ggaaaccgac
     6901 gcgtccggtc aaacaaaagg taagccgtcg tcgttacgaa gatgacgatg attacgacga
     6961 ttatgatgac tatgaggatg aagaaccgat gccgcgcaaa ggtaagggca aaggcaaagg
     7021 gcgtaagcct cgtggcaaac gcggctggct atggctactg ctaaaactgg ctatcgtttt
     7081 tgccgtgctg atcgccattt acggcgttta tctcgatcaa aaaattcgta gccgtattga
     7141 tggcaaggtc tggcaactgc ctgcggcagt ttatggccga atggtcaatc ttgagccaga
     7201 tatgaccatc agcaagaacg agatggtgaa gctgctggag gcgactcagt atcgtcaggt
     7261 gtcgaaaatg acccgtcctg gcgaatttac cgtgcaggcc aacagcattg agatgatccg
     7321 ccgtccgttt gatttcccgg acagtaaaga agggcaggtg cgtgcgcatc tgacctttga
     7381 tggtgatcat ctggcgacca tcgtcaatat ggagaacaac cgtcagttcg gctttttccg
     7441 tcttgatccg cgtctgatca ccatgatctc ctcgccaaac ggtgagcaac gtctgtttgt
     7501 gccgcgcagt ggtttccctg atttgctggt ggatactttg ctggcgacag aagaccgcca
     7561 tttctacgag catgatggga tcagcctcta ctcaattggg cgtgcggtgc tggcaaacct
     7621 gaccgcaggt cgcacggtgc agggtgcgag tacgctgacg caacagctgg tgaaaaacct
     7681 gttcctctcc agcgagcgct cttactggcg taaagcgaac gaagcctaca tggcgctgat
     7741 catggacgcg cgttacagca aagaccgtat tcttgagctg tatatgaacg aggtgtatct
     7801 cggtcagagc ggcgacaacg aaatccgcgg cttcccgctg gcaagcttgt attactttgg
     7861 tcgcccggta gaagagctaa gcctcgacca gcaggcgctg ttagtcggta tggtgaaagg
     7921 ggcgtccatc tacaacccgt ggcgtaaccc gaaactggcg ctggagcgac gtaatctggt
     7981 gctgcgtctg ctgcaacagc aacagattat tgatcaagaa ctctatgaca tgttgagtgc
     8041 ccgtccgctg ggggttcagc cgcgcggtgg ggtgatctct cctcagccag cctttatgca
     8101 actggtgcgt caggagctgc aggcaaaact gggcgataag gtaaaagatc tctccggcgt
     8161 gaagatcttc actacctttg actcggtggc ccaggacgcg gcagaaaaag ccgccgtgga
     8221 aggcattccg gcactgaaga aacagcgtaa gttgagcgat cttgaaactg cgattgtggt
     8281 cgtcgaccgc tttagtggtg aagttcgtgc gatggtggga ggttctgagc cgcagtttgc
     8341 gggctacaac cgtgcgatgc aggcgcgtcg ttcgattggt tcccttgcaa aaccagcgac
     8401 ttatctgacg gctttaagcc agccgaaaat ctatcgcctg aacacgtgga ttgccgatgc
     8461 gccaatcgcg ctgcgtcagc cgaatggcct ggtctggtca ccgcagaacg atgaccgtcg
     8521 ttatagcgaa agcggcagag tgatgctggt ggatgcgttg acccgttcga tgaacgtgcc
     8581 gacggtaaat ctggggatgg cgctggggct gcctgcagtt acggatacct ggatcaaact
     8641 gggcgtaccg aaagatcagc tgcatccggt tccggcgatg ctgctggggg cgttgaactt
     8701 aacgccaatc gaagtggcgc aggcgttcca gaccatcgcc agcggtggta accgtgcacc
     8761 gctttctgca ctgcgttcgg taatcgcgga agatggcaag gtgctgtatc agagcttccc
     8821 gcaggcagaa cgcgctgttc cggcgcaggc ggcgtatctg acactatgga ccatgcagca
     8881 ggtggtacaa cgtggtacgg gtcgtcagct tggggcgaaa tacccgaacc tgcatctggc
     8941 agggaaaaca gggactacca acaataacgt agatacttgg tttgcaggca ttgacggcag
     9001 cacggtgacc atcacctggg caggccgtga taacaaccag ccgaccaaac tgtatggagc
     9061 cagcggggca atgtcgattt atcagcgtta tctggctaac cagacgccaa cgccgctgaa
     9121 tcttgttccg ccagaagata ttgcagatat gggcgtggac tacgacggca actttgtttg
     9181 cagcggtggc atgcgtgtct tgccggtctg gaccagcgat ccgcaatcgc tgtgccagca
     9241 gagcgagatg cagcagcagc cgtcaggcaa tccgtttgat cagtcttctc agccgcaaca
     9301 gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat
     9361 gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa
     9421 ataacatccc atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca
     9481 ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct
     9541 ttataataat cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc
     9601 gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac
     9661 agcggttagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat
     9721 caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc
     9781 ggcgaagcac tctgctactg cgactaaaac ggatacacca attgaaaaaa cgccgcagtc
     9841 tatttcggtt gtgacaaatg aagagatgca gatgcatcaa tttcagtctg taaaagaagc
     9901 attaggttat acacctggcg ttacggttag cagtcgtggt gcttctaata catatgattt
     9961 tgttatcatt cgtggtttct catctgttgg tctgaatcaa aataattacc ttgatggact
    10021 aaaacttcag ggcaactttt ataacgatgc tgtgattgat ccttacatgc tcgagagggt
    10081 tgaactgatg cgtgggccga cgtctgttct ctatggaaaa agtaatcctg gtgggattat
    10141 ttctatggtg agtaagcgtc cgaccactga acccctgaaa gaaattcagt ttaaaatggg
    10201 gacggataat ctgtttcaga ccggattcga tttcagtgat gcactggatg ataacggtga
    10261 gttctcttac cgtttgacgg gcctcgctcg ctcaacaaac gaacagcaga aaaactctga
    10321 atctcagcgt tataccattg ctccatcatt ctcatggcgt ccagacgaca aaaccaattt
    10381 tactttcctg tcctatttcc agaatgaacc agaaacgggt tattacggtt ggttgccgaa
    10441 agaggggacc gttgagccat tgcctaatgg taagcgtcta ccgactgact tcaatgaagg
    10501 tgcatcgaat aatacatact cccgtaacca gaaaatggtg ggatatagtt ttgaacatgg
    10561 ttttaatgac accttcaccg tgcgtcagaa tctgcgtttc agtgaaatga aaacctcaca
    10621 gaaaagtgtt tatggcacag ggattgctgg tgatggtcat accctaaacc gcgggacagt
    10681 ggtggataat gagcgtctac aaaactttag cgttgatacc caacttgaaa gtaagtttgc
    10741 tacaggtgac gtcgagcata ctttgctgac aggggtagac ttcatgcgga tgcgtaatga
    10801 tatcaatgcc agctttggat ccgcaccatc catcgatctt tataacaaat atcaccctga
    10861 atactttgca tttggtagcg cagagccata ccagatgaat gaaagcaaac aaacaggtat
    10921 ttatgttcag gatcaggcgg aatggaataa atgggtattc actctggggg gacgctacga
    10981 ttggtctaag caagcgacca ctgttcgtga aaactcttat acgccgactg aaggttatat
    11041 tgagcgcaat gatcatcagt tcacctggcg cggtggtgta aattacttat tcgataatgg
    11101 tatttcacct tactttagct atagccagtc ctttgaaccg agtgctttcg atctgtggag
    11161 caacccacgc atttcctata agccatcgaa aggtgaacag tatgaagctg gcgtaaaata
    11221 tgttccgaat gatatgccgg tcgttgttac gggcgcagtc tatcaattga cgaaaacaaa
    11281 taacctgaca gcagacccaa caaacccatt agcgcaagtc ccagcaggtg agattcgcgc
    11341 tcgtggtgtg gaacttgaag caaaagcggc gttaaatgcc aatattaact tgacggcttc
    11401 ttatacctac accgatgcgg aatacaccaa agacaccaat ctcaaaggta aaactccaga
    11461 acaagtaccg gagcatatgg catctctctg gggggattat accttcaatg aagggccgct
    11521 ttctggttta acattgggaa caggtggtcg ttttattggt tccagctatg gtgatccggc
    11581 aaacactttt aaagtgggta gcgcagctgt aatggatgct gttgtaaaat atgatctggc
    11641 acgctttggt atggcgggat ccagccttgc tgtgaacgtc aacaatttgc tcgatcgtga
    11701 gtatgttgcc agttgcttcc agacttatgg ctgcttctgg ggcgcagaac gtcaggtcgt
    11761 tgcaaccgca accttccgtt tctaatttct cttttggggc acggatttcc gtgcccattt
    11821 cacaagttgg ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt
    11881 aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct
    11941 gccgggaaag tgaccggtct gattggtcac aacggttcta gtaaatccac tctgctcaaa
    12001 atgcttggcc gtcatcagcc tccgtcagaa ggggagattc ttcttgatgc ccagccgctg
    12061 gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt atttgccgca gcagcttcct
    12121 ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc
    12181 gcgctggggc gctttggggc ggcagatcgc gaaaaagtcg aggaagctat ctcgctggtt
    12241 ggcttaaaac cgctggcgca tcggctggtt gatagtctct ctggcggcga acgtcagcgg
    12301 gcgtggatcg ccatgctggt ggcgcaggat agccgttgtc tgttgctcga cgaaccgacc
    12361 tcggcgctgg atatcgccca ccaggttgat gtgctggcgc tggtgcaccg tttaagtcag
    12421 gagcgtggcc tgacggtcat tgccgtgttg cacgatatca atatggcggc acgctactgt
    12481 gattatctgg tcgccctgcg cggtggtgaa atgattgctc agggaacgcc tgcggaaatt
    12541 atgcgcggcg ataccctcga aatgatttat ggcatcccga tgggtatttt gccgcatccg
    12601 gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc
    12661 gtcgactgtt aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg
    12721 cggcggctat tgatcccaat cgtattgtgg cgctggagtg gttgccggtg gaattactgc
    12781 tggcgctcgg catcgtgcct tacggcgtag cggataccat caactatcgc ctgtgggtca
    12841 gcgaaccacc attgccggac tcagtgatcg acgtcggttt gcgcacagaa cctaaccttg
    12901 aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat ggcccttcag
    12961 cagaaatgct ggcacgtatt gcgccggggc gtggatttaa cttcagcgac ggcaaacagc
    13021 cgctggcgat ggcgcgtaaa tcactgacgg aaatggcaga tttacttaac ctgcaaagcg
    13081 cagcagaaac gcatttagcg caatatgaag actttatccg cagcatgaaa ccccgctttg
    13141 tgaagcgtgg tgcacgcccg ttattgctga cgacgcttat cgatccgcgc catatgttgg
    13201 tcttcggtcc aaacagcttg ttccaggaaa ttcttgatga gtacggcatc ccaaatgcct
    13261 ggcaagggga aaccaacttc tggggcagta ccgccgtcag tatcgatcgt ctggcagcgt
    13321 ataaagacgt tgatgtgctc tgttttgatc acgacaacag caaagacatg gatgcgctaa
    13381 tggcaacccc gctgtggcag gccatgccgt ttgtccgcgc cggacgcttt cagcgcgtac
    13441 ccgcagtctg gttttatggt gcgacgctct cggcaatgca ctttgtgcgc gttctggata
    13501 acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt ttcccggcgt tattgctggc
    13561 gctgctggtg attgtcgcta cggcgctgac ctggatgaac ttttcgcagg cgctgccgcg
    13621 tagccagtgg gcgcaggccg cctggtcgcc ggatattgac gtcatcgagc agatgatttt
    13681 tcactacagc ttgctgccgc gtctggcgat ttcgctgctg gtgggcgcag gcctggggct
    13741 ggtgggcgtg ctgtttcagc aagtgctgcg taatccgctg gcggaaccga cgacacttgg
    13801 cgttgctaca ggcgcgcaac tggggattac cgtcactacg ctctgggcga tcccaggggc
    13861 gatggcgagt cagtttgctg cgctggcggg cgcttgcgtt gttggcttaa tcgtctttgg
    13921 cgtcgcgtgg gggaaacggc tttcgccggt aacgctgatc ctcgcggggc tggtagtgag
    13981 cctttattgt ggggcaatca atcagttact ggttatcttc caccatgacc aactgcaaag
    14041 catgttcctg tggagcaccg gaacgctgac gcaaaccgac tggggcggtg ttgagcgttt
    14101 atggccgcag ttgctgggcg gtgtgatgct gacgttgctg ctacttcgcc cgttaaccct
    14161 gatggggctt gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc tcgcgcgtct
    14221 ggcggcgctg tcgctggcga ttgtcatcag tgcgctgctg gtgaacgcgg tggggattat
    14281 cggctttatc gggttgtttg cgccgttgct ggcaaaaatg ctgggggcgc ggcgtctgct
    14341 gccgcgactg atgctggcgt cgttgattgg tgcgctgatc ctctggcttt ccgatcaaat
    14401 catcctctgg ctgactcgcg tgtggatgga agtgtccacc ggttcggtca ctgcgttgat
    14461 cggtgcgccg ctgctactgt ggctgttgcc gcgtttacgc agcattagcg cgccggatat
    14521 gaaggtcaac gatcgtgtcg cggctgaacg ccaacatgtg ctggcgtttg ccctcgcggg
    14581 cggcgtgctg ctgttgatgg ctgtggtggt ggcgctgtcg tttggtcgtg atgcgcacgg
    14641 ctggatgtgg gcgagcgggg cgttgctcga tgatttaatg ccctggcgct ggccgcgaat
    14701 tatggcggcg ctatttgcgg gcgtcatgct ggcggtggcg ggctgtatta ttcagcgact
    14761 gaccggcaac ccgatggcaa gcccggaagt gctggggatt agctccggcg cagcgtttgg
    14821 cgtggtactg atgctgtttc tggtaccggg taatgctttt ggctggctgt tacccgcggg
    14881 aagtatcggt gccgcggtga cgctgttgat tattatgatc gccgccgggc gcggtggatt
    14941 ttccccacac cgcatgttac tggcggggat ggcgttaagt accgcattca ccatgctttt
    15001 aatgatgttg caggcgagtg gtgacccgcg aatggcgcaa gtgctcacct ggatctctgg
    15061 ttcgacctac aacgccaccg atgcgcaggt ctggcgtacc ggaattgtga tggtgatttt
    15121 gctggcgatt accccgctgt accgccgctg gctgaccatt ttacctctgg gcggtgatac
    15181 cgcacgagct gtgggaatgg cgctgacgcc gacgcgaatt gcgctgctgc tgttagcggc
    15241 ttgcctgacg gcgacctcga cgatgactat tggaccgttg agttttgttg gtttaatggc
    15301 accgcatatt gcgcggatga tgggttttcg acggacgatg ccacacatcg taatttcggc
    15361 gctggtgggt ggtttactgc tggtattcgc tgactggtgt gggcggatgg tgctgtttcc
    15421 attccagatc ccggcggggc tgctgtcgac ctttatcggc gcgccatatt ttatctattt
    15481 gttgagaaag cagagccgtt aattgagtgc ctcgcccggt gggcgaggca aaattatcac
    15541 aacttcgcaa atacccgacg tgcagcatcg atggtgttat tgatatcttc catgctgtgc
    15601 gccacagaca taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc ttcgtccagc
    15661 atcatatgga agaaacgctt aaagcgttcc acgtcacagg ccatcacatc ctgatagcac
    15721 gtcacggact cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg gttaacgacc
    15781 agcggagttc cggcttcttc tgccgcttcc agcagacctt ctgccagacg tgttgtcagc
    15841 tcatccagcg tttcgtgaac gcccggctgc gcgacttcat tcagacaggc gaaacccgct
    15901 gccatcgcaa tcgggttacc ggaaagcgta cccgcctgat agaccggacc cgtcggggcc
    15961 agcgcatcca ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg
    16021 attttgccga ggcaggttaa atctggcact acgccgtaat aatcctgtgc gccagccagt
    16081 gccacgcgga agccggtcat cacttcatcg atgatcagca acgcgccaaa ttcgtcgcac
    16141 agcgcacgca gacctggcag gaactctggc tgcggcggaa cacagttcat attgcctgcc
    16201 accggctcga cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca
    16261 gaagccagat cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacaccc
    16321 ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca
    16381 gcgtgaccgt ggtaacaacc ttcaaattta atgattttgt cgcgaccggt aaaaccacgg
    16441 gccaggcgga tggcgctcat ggtcgcttca gtgccggagt tcaccatgcg caccatatcc
    16501 atggtcggga ccagttcggt caccagttgc gccattttca cttccatttc ggtcggtgca
    16561 ccaaagctta aaccacgctc ggcggcttca atcacggcat tgcggatagc cggatggtta
    16621 tggcccagca ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca
    16681 tcgtacagat aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta
    16741 aaggcgcgaa caggggagtt cacaccgcca gggagcagct cgcgcgctgc gctgtaaaga
    16801 ttttcagact tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct
    16861 atgttattca taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc
    16921 ctgatttctt agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt
    16981 tcatggaacg tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg
    17041 gatgaaaact gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca
    17101 actgattcgc caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc
    17161 agtcgtcggc acgcttgtcg ggctggcagc ggttgctttt gacaaaggtg tcgcctggtt
    17221 gcagaaccaa cgtatggggg cgctggtaca tactgctgat aattatccgc ttctgttaac
    17281 cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata
    17341 cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa ggggcgctgg aagatcaacg
    17401 tcccgttcgc tggtggcgtg tgttgccggt gaaattcttt ggcgggctgg ggacactcgg
    17461 cggaggcatg gtgctggggc gcgaagggcc aaccgtgcag atcggcggca atatcggtcg
    17521 catggtactc gatattttcc gcctgaaagg tgatgaagct cgccatacgc tgctggcaac
    17581 cggtgctgct gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat
    17641 tatcgaagag atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat
    17701 tggcgtcatt atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga
    17761 cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat
    17821 tatttttggc attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct
    17881 gcaccgtgtg cacggcggca atattaccaa atgggtgcta atgggcggtg cgattggcgg
    17941 tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt ttaacctgat
    18001 tcctatcgct accgcaggga atttcagcat gggaatgctg gtgtttatct tcgtcgcgcg
    18061 ggtcattacc accttactgt gcttctcttc cggcgcgccg ggcggtattt ttgccccgat
    18121 gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg gttgtcgttg agctgtttcc
    18181 gcaatatcac cttgaggcgg ggacttttgc tattgccgga atgggggcgt tactggcggc
    18241 atctattcgc gcgccgttaa cggggatcat tttggttctg gagatgaccg ataactacca
    18301 gctcattttg ccaatgatta ttaccggtct tggcgcaaca ctattagcgc aatttaccgg
    18361 cgggaaaccg ctatattcgg agattcttgc gcgcacgctg gcaaaacagg aagctgagca
    18421 actggcgcga agcaaggccg catcagccag cgagaatact tgaacgaaat accagggtat
    18481 tagataatgg cgattattat tgggttagaa tttgcccaat tgccgatgtc gtttggagca
    18541 aaatatgagt gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt
    18601 taaaagcctg atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg
    18661 tggcggttgc agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga
    18721 tatgaccatc gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc tgcaatatct
    18781 ggtcggtggt tccgttgact ataccgaagg actggaaggt tctcgtttca tcgtgaccaa
    18841 cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt agcatctaat ctgttgttca
    18901 tgtgccggat aaactatccg gcacatcttc tctcttcaac gcccattctc atccagcgca
    18961 aacgtcggta gcttaagatg ccaacgaata gccgccagcc gaatcaatag cgtcacgacc
    19021 atgcccatca tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg
    19081 acaataccgc cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatt
    19141 tcgcgggcca gtacatcacg aatgatcccg ccgccaacgc cagtaatgac gcccatacaa
    19201 accgcgatta acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac
    19261 accgccagac caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg
    19321 cgcaccagca cgatggtcag catgctggtt accattgcaa cgaccagatc ggtgggatct
    19381 ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc gaattgtccc gccgcctaca
    19441 gcggtaacca cgcccagtac cagaacacca aaagggtcca tacgcaattt tccggctaac
    19501 aaaacgccgg agatggcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt
    19561 gttcaatccc cactgagcac ctaaaaaggt caggcgctaa tctacctgtg aaagcgcatt
    19621 acagagctgt tgtgcagcga ggataatacg tgggcttgca cgtcaaacca gtcactcgtg
    19681 agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt aggaatttgg
    19741 tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg ctaacacctg ttcgcggcta
    19801 acttgcggcc aaggaacccg gctgtcttta aagatgtttt ctccgccaca aacttcgagt
    19861 acctggtttt gaatcgactc ttttccactg gtaaatggcg gattaatgcc gaattgcaga
    19921 aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta ctgatccagc
    19981 agggattgcg cggcttgttc ggccttatcc ggttgcggac tccagggggc cagttgacgt
    20041 aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt tattcccggc
    20101 gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat caccagatcg
    20161 ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac ctgctcaatc
    20221 ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt gatcccggcg
    20281 gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggtgc cgcgttgagc
    20341 cacagtggcg cgagaaaaga cagggcgacc agcgccctga acagtgactt agccatgtgc
    20401 cagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa cagccaggaa
    20461 ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc gtacgacaac
    20521 aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca tctctacagc
    20581 aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt tgatgaaagc
    20641 gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa tgcaggcctc
    20701 agcggcagcg atcagtttat cgtcagcttc aaagcctgcc ggacagcctg gtaactgacc
    20761 gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgtgcttcgt ccgagacaac
    20821 gatatcgccc actttcaacg ttggtgccag gccaccggca gaaccggtgt taataatcac
    20881 atctggcttg cagtgttcca acagcaaagt ggcacccagc gccgcagcga ctttaccgat
    20941 gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga tttcgcaacc
    21001 gccgagactg atagtttgac gattttcgat tttgtcacgc agcagcgtaa cttcttcttc
    21061 cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga tttgaaggca
    21121 tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac atggcacaga
    21181 ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa
    21241 ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg
    21301 caattcgtcg tctgcaacaa aagacccagg tttttccact ggagcgcaat gccgccgtgc
    21361 gcacgcgtct tacccactcg atggaagtcc agcaggtggg gcgctacatc gccaaagaaa
    21421 ttctaagccg tatgaaagag cttaaattac tggaagcata cggcctggat gaactgaccg
    21481 gcccctttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc aatccgccgt
    21541 ttggtcattt tggcgaagcg gcgataaatg attggtttcg ccagcgtttg cacccggaag
    21601 atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggcacta cgtttacggg
    21661 acggggaaga accgcttaac gagctgcggc gcaagattcg tcaggactta tgtcattttg
    21721 aggggaatgc acaaggcatt cgtctggtgc atacattgat gaggatgaat ctcacctggg
    21781 cacaggttgg cggtatttta aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg
    21841 agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa gcctatattg
    21901 cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta acgtggatta
    21961 tggaagcagc cgacgacatc tcctattgtg tggcagacct tgaagatgcg gtagagaaaa
    22021 gaatatttac cgttgagcag ctttatcatc atttgcacga agcgtggggc cagcatgaga
    22081 aaggttcgct cttttcgctg gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt
    22141 taagccgcag tacggaagat cagtttttta tgtatttacg ggtaaacacc ctaaataaac
    22201 tggtacccta tgcggcacaa cgatttattg ataatctgcc tgcgattttc gccggaacgt
    22261 ttaatcatgc attattggaa gatgccagcg aatgcagcga tcttcttaag ctatataaaa
    22321 atgtcgctgt aaaacatgtg tttagccatc cagatgtcga gcagcttgaa ttgcagggct
    22381 atcgggtcat tagcggatta ttagagattt atcgtccttt attaagcctg tcgttatccg
    22441 actttactga actggtagaa aaagaacggg tgaaacgttt ccctattgaa tcgcgcttat
    22501 tccacaaact ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt aaattaccgt
    22561 cagattctcc tgagtttccg ctatgggaat attattaccg ttgccgcctg ctgcaggatt
    22621 atatcagcgg tatgaccgac ctctatgcgt gggatgaata ccgacgtctg atggccgtag
    22681 aacaataacc aggcttttgt aaagacgaac aataaatttt taccttttgc agaaacttta
    22741 gttcggaact tcgcgctata aaacgaatct gaagaacaca gcaattttgc gttatctgtt
    22801 aatcgagact gaaatacatg aaaaaaacca cattagcact gagtgcactg gctctgagtt
    22861 taggtttggc gttatctccg ctctctgcaa cggcggctga gacttcttca gcaacgacag
    22921 cccagcagat gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca
    22981 ttaacgtaga aggtagcaca accgttaata cgccgcgtat gccgcgtaat ttccagcagt
    23041 tcttcggtga tgattctccg ttctgccagg aaggttctcc gttccagagc tctccgttct
    23101 gccagggggg ccagggcggt aatggtggcg gccagcaaca gaaattcatg gcgctgggtt
    23161 ccggcgtcat cattgatgcc gataaaggct atgtcgtcac caacaaccac gttgttgata
    23221 acgcgacggt gattaaagtc cagctgagcg atggccgtaa gttcgacgcg aagatggtcg
    23281 gcaaagatcc gcgctctgat atcgcgctga tccaaatcca gaacccgaaa aacctgaccg
    23341 caattaagat ggcggattct gatgcactgc gcgtgggtga ttacaccgta gcgattggta
    23401 acccgtttgg tctgggcgag acggtaactt ccgggattgt ctctgcgctg gggcgtagcg
    23461 gcctgaatgc tgaaaactac gaaaacttca tccagaccga tgcagcgatc aaccgtggta
    23521 actccggtgg tgcgctggtt aacctgaacg gcgaactgat cggtatcaac accgcgatcc
    23581 tcgcaccgga cggcggcaac atcggtatcg gttttgcaat cccgagcaac atggtgaaaa
    23641 acctgacctc gcagatggtg gaatacggcc aggtgaaacg cggtgagctg ggtattatgg
    23701 ggactgagct gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt
    23761 tcgtaagcca ggttctgcct aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg
    23821 tgatcacctc actgaacggt aagccgatca gcagctttgc cgcactgcgt gctcaggtgg
    23881 gtactatgcc ggtgggcagc aaactgaccc tgggcttact gcgcgacggt aagcaggtta
    23941 acgtgaacct ggaactgcag cagagcagcc agaatcaggt tgattccagc tccatcttca
    24001 acggcattga aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca
    24061 acgtgaaaac gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg
    24121 gcgcgaacca gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc gacagcaaac
    24181 cgtctgtgct ggcactgaac attcagcgcg gcgacagcac catctacctg ttaatgcagt
    24241 aatctcactc aaccccttcc tgaaaacggg aaggggttct ccttacaatc tgtgaacttc
    24301 accacaactc catacatctt catcatcctt taggcatttg cacaatgccg tacgttacgt
    24361 acttccttat gctaagccgt gcataacgga ggacttatgg ctggctggca tcttgatacc
    24421 aaaatggcgc aggatatcgt ggcacgtacc atgcgcatca tcgataccaa tatcaacgta
    24481 atggatgccc gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac
    24541 gaaggtgcat tgctggtact ttcacaggga cgagtcgtcg atatcgatga cgcggtggca
    24601 cgtcatctgc acggtgtgcg gcaagggatt aatctaccgt tacggctgga aggtgaaatt
    24661 gtcggcgtaa ttggcctgac aggtgaacca gagaatctgc gtaaatatgg cgaactggtc
    24721 tgcatgacgg ctgaaatgat gctggaacag tcgcggttga tgcacttgtt ggcgcaggat
    24781 agccgtttgc gggaggaact ggtgatgaac ctgattcagg cagaggagaa tactcccgca
    24841 cttactgaat gggcgcaacg gctggggatc gatctcaatc aaccgcgagt ggtggctatt
    24901 gttgaggtcg acagcggtca gcttggtgtg gacagcgcaa tggcggagtt acaacaactg
    24961 caaaacgcgc tgactacgcc cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa
    25021 atggtggtgt tgaaaccggc gttgaactct tttgggcgct gggatgcaga agatcatcgt
    25081 aagcgagttg aacaactgat tacccgcatg aaagagtacg gccggctgcg ttttcgcgtt
    25141 tcactgggca actattttac cggtcctggc agtattgccc gatcctatcg tacggcgaaa
    25201 acgacgatgg tggtgggtaa acagcggatg ccagaaagtc gctgctattt ttatcaggat
    25261 ctgatgttac ctgtgttact cgacagtttg cgtggcgact ggcaggccaa cgaactggcg
    25321 cgaccgctgg cgcggctgaa agcgatggac aataacggct tgctgcgacg aacgctggcg
    25381 gcgtggtttc gtcacaatgt gcaaccgctg gcaacgtcaa aggcgttgtt tatttatcgt
    25441 aataccctgg agtatcggct taatcgtata tcggaactga ccgggcttga tttgggcaat
    25501 tttgatgaca ggttgctgct gtatgtggcg ttacaactgg atgaagagcg gtaggttatg
    25561 cgttaaggtt gaggcggcgc tgtctcattc ccctcagccc gaaggggaga ggggaatgcc
    25621 cgaattaacc gtcaattcgg gattatttac gcgttagttt ttccagatcg gcttcaatct
    25681 cgctgatctt attggttacg actgactcca ggtgacgtaa gtcgtcgagg atcttacgct
    25741 taagatcaac ttcgctgcgg tcacgctggc agatttgatc aagctcatca atgatatacc
    25801 gtagattcgg gctgatttcc tggacttctt tataaccctg acccacacca tcagcgacga
    25861 ccgttttacg ctgacgcgga tatttaaact taacgctctt ggcgaaaaac tcgcctttgt
    25921 ctttctggaa atagattttc agaatatcgt tgttggcttc ctgccggagg ctgtaacgat
    25981 caatttcttc aggattggta atacccagac ttttcagatt gtcgtacata gcgttacctc
    26041 aaaatgagtc agtaaattgt gcttatttta gcatttggcc tagcccgccc ggctcgactg
    26101 tgatcgcaga aagccttttt cttccagact tctatcatgg cgtaaaaatc aaaaaattac
    26161 ctgctttatt gtggtgataa aattcacgtt ctacaccagt tccagcatcg ttacttccgg
    26221 gcggcagttc agacgcaaac catacaaact gcccacgcca cgggttgtgt agatgtgtcg
    26281 ttcgccaaag gcatttaatc cggcgacgta acgtttatct tcgacagggg caaaaggttc
    26341 accaaccaac ggtacgcgca gttgcccgcc gtgagtatgg ccgcacagca tcagatccca
    26401 gggttcatca cgcatgactt ctttgctgtc gggattatgt gccagcatca gtcttggcag
    26461 atttgcttcg ctggcgggag gtggtttgca ttgtccggcc cataagtcac cggtgcctac
    26521 cagttcgaat tgcctgttcg gcgtggcgat caccgtagcc tggttaaaca acaccgtgat
    26581 gcccactgat ttcaacgtct cgccaattaa gtgatttttt tctgtaccaa cagggcgatc
    26641 gtggttgccg aagcaggcaa acgtcggcgc acattcggca aggggggaga ggacatcact
    26701 aaacgccgaa aaattcagcg gcatatcaaa taatacgtaa tcgccgccca gtaatatcaa
    26761 atcgggcttt tgttctatgc caagagcaat cgcgtcagaa atcaggctta aaggaacaaa
    26821 acgagagtaa tggagatcgg ccagaaaaag aattttgaat ggtgctgcgt tgtctttaaa
    26881 aaaggcgagg cggtgacgga ttaattcaaa ccagcctggc tcccagtaat gcatataacc
    26941 gaaacctgag ctcgtggcta tcgtcgcggc agtagcctgc aacaatcggc ggcgtgaaat
    27001 catcgctcat ccctgcaaaa aaaatcgggc agcgtcgtgc tgcccgtgtg catactttta
    27061 gtcgatggta cgcagcagtt cgttaatgcc gactttgccg cgagttttcg cgtcaacttt
    27121 cttaacgata accgcacagt agaggctgta tttgccatct tttgacggca gattacctga
    27181 aacaaccaca gaccccgccg gaacgtgacc gtagtggatt tcgccggttt cacggtcgta
    27241 aatacgggtg ctctgaccaa tgtatacgcc catggaaatg acggaacctt cttcgacaat
    27301 caccccttca actacttcag agcgtgcgcc aatgaagcag ttatcttcaa tgatggttgg
    27361 gttagcctgc agcggttcca gcacgccgcc gatgcccacg ccaccggaaa ggtggacgtt
    27421 tttaccaatc tgcgcacaag aaccgacggt cgcccaggta tcaaccatgg tgccttcatc
    27481 aacatatgcg ccgatgttga cgtaagacgg catcagcacg gtgttacggg caataaacgc
    27541 accctgacgt accgccgctg gtggcacaac gcggaagcct tctttctgga aacgtgcttc
    27601 gtcgtagtcg gcgaatttca ttggcacttt gtcgaagtag cggctttctg ccccttcgat
    27661 cacctgatta tcattaatac ggaaagagag cagcaccgct tttttcaacc actgatgcgt
    27721 cacccactga ccgtcaattt tttccgctac acgcagtgca ccggaatcca gcagggcgat
    27781 cacctgattt accgcttcgc gggtaacggt gtctgcgttg gttggcgtga tctcggcacg
    27841 gcgttcaaaa gcggtttcaa taatgttctg taactgctgc attgttaaac tcttttcata
    27901 tcagtaaaca catcaccctt tatcgtttgg attgagggcc tctgtcaacc gctgatgcac
    27961 ttcctgctgc aactcgttat taagcgcacg ccggtcagcg gtggcaatta tgaataaatc
    28021 ttctactcgc tcgccaatgg ttgtaattcg ggcaccatga agcgaaattc ccagatcggc
    28081 aaaaattttc ccgactctcg ccagcagtcc tggctggtcg agcgcgataa gctcaaggaa
    28141 cgatttgcgg tcggtatggg tcggcaaaaa cgttacttcg gtttcaacag taaaatggcg
    28201 taatttggcg ggttggcgac ggggctgcgg tggctgccag ctactttgcg tcagtacttg
    28261 ctccagacca aaccgaataa cctcatgacg atctgcggac agcgggctgc catcgggttc
    28321 cagcacgata aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg
    28381 aacacttaaa ttgcggcggt ctaattcggc acagacggcg gcaaacagat aggggcggtc
    28441 cgggctccag ataaaaatct cggtgcctcc acgcgtagcc tgcgggctaa gcaataccag
    28501 tggtttgctt aaatcatgct gtaataaatg gcgggcatgc caggccagtt gatttgggct
    28561 atggcggaca aaatagttag cacgacagcg tgaccaaatt tggtgcagcg cctcttcgtc
    28621 gatgttatcc atgcgcagta gtgccagtgc ctggagttgg tgatggcgaa cccgttcgcg
    28681 catatccggc gtgttttgca ttccgcgtcg tagctgcttt tcggtggcaa aatagagttc
    28741 gcgcaacaga ctttgcttcc agctattcca cagcgtttcg ttggtggcgc aaatgtcagc
    28801 cactgtcagg cataccagat agcgcagacg attttccgtt tgaacttctt cggcaaactg
    28861 cttgatgact tcagggtcct gaatatcgcg gcgttgtgct gtcaccgaca tcagcaagtg
    28921 ctggcgaacc agccaggcaa ccagctgtgt ttcgcgtgag ttcagcccgt ggagttctgc
    28981 aaaatgcact acatcctgag caccgagaat ggagtggtcg ccgccgcgtc ctttggcgat
    29041 atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg gccagacgtc
    29101 cacacacaac ggatggcgct ggcgcgtttc ttcactggca aaactctcca gtttcagcat
    29161 cacgcggata gtatgttcat ccaccgtgta ggcgtggaac agatcaaact gcatctgccc
    29221 gacgatatgc gaccattgcg gcatatacgc gccgagcacg ctatggcgat gcattggcaa
    29281 tagcccgcgc cgcaccgctc cggggtgacg cagaatgctc aaaaacagtt ttcgtgcttc
    29341 cggaatatta cacagcggtt gttgcagatg gcgacgggca tggcgtaact ggcgcagcgt
    29401 ggtggagtaa atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat
    29461 ggcttccggc tggcgcataa atagtgtttc atcacgcagg tcgattagcg taccgcgtag
    29521 ctgaaactca tcgtcgattg gacgtggttt ttcgtcggca ggaagggcga ggatggcttc
    29581 atcgaacagt tgcagcagca tctggttgag ttcactgacg cggcgtgtaa cgcggaagta
    29641 atccttcatc atccgctcga ccggttcgtt gccttcacca ctgtaattca gacgctgagc
    29701 gacgctaagt tggcgatcga ataacaggcg attatcgtaa cggctgacga ccagatgcag
    29761 ggcaaagcga atacgccaca gtatatgcag acattcgttt aattccgccc gctccgctga
    29821 ggttaagaag ccaaacccaa ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc
    29881 cacccattgc agagtgtgga tatcgcgcaa gccgccaggg ctacttttga tgtctggttc
    29941 aagattgtag ctggtgccgt ggtaacgctg atggcgctgg ttctgttctt caactttcgc
    30001 cgcgtagaac ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt gcagttcgag
    30061 gaagagcgca acatcgccaa ttaataagcg ggattcgatt aaattggtgg cgacggttaa
    30121 atccgataac ccttccagca tgcactcttc aagcgtgcgc acactatgac cgacttccag
    30181 ctttacatcc cagagcagcg ttaacagctc gcccactttt tgcgcctgat cgtccgggag
    30241 ctttttacgg cttaaaatca gtaaatcgat gtcagaaagt ggatgcagct cgccacgacc
    30301 gtagccaccg acggcgacca atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca
    30361 taatcgttgc aggagctggt cgataaactc ggtgcgcgcc tcaatcaact gttctgcaga
    30421 gatcccattg tcaaaggcat cacccagcca acgctggaaa gtatcgatat gggcttttat
    30481 cccaccgacg gttaattcat cacggggcca gacgcatgga ttttgcggtt gaccgggcag
    30541 ggtggggaga gcggtgtttg cgtactgttc tggaagggta ttcattgtgc gccactcata
    30601 agattaaatt atcgcattaa aaaagccggc attcgccggc ttcatcttat tcgtcgtgcg
    30661 agattatcgc cgggatggtg tcatccttgc gtagcgtcag aatttcgcag ccgttatcag
    30721 tcaccacaat agtatgctca tattgtgcag acaagctgcg atctttggtt tttaccgtcc
    30781 agccatcttt catggtgcgg atctcttttt tacccgcgtt gaccattggc tcgatggtga
    30841 acgtcatccc aggtttcagt acgacgttgg tttcacggga gtcatagtgc agcacctgcg
    30901 gttcttcatg gaagccgcga ccaataccgt gtccgcaata ttcacgaacg acggagaagc
    30961 cttctgcttc gacaaacttc tgaatcgccg caccgatttc gcgcaggtta atgcctggtt
    31021 tgaccatgcg cagcgccagg tacaggcttt cttgcgtgat gcggcacaga cgttcgccca
    31081 tgatggtcgg cttaccgacg ataaacattt tcgaggtatc gccgtggaaa ccatctttga
    31141 ttacggtgac atcaatgtta acgatatcgc catctttcag cagcttcgca tcgtccggga
    31201 taccgtggca caccacttca ttaatagaga tgcaaacgga tttcggatag ccgtgatagc
    31261 cgaggcaggc agaaaccgcg tgttgttcat taacaatgta atcattacag atgcgatcca
    31321 gctcgccggt gctgacgccc ggtttaacat acggttcgat catctccagc acttcggcag
    31381 ccagtcggcc agcgacgcgc attttttcga tatcttctgg ggtcttgatt gagatagcca
    31441 ttaattctgt ccatcagcgt cggtgatacc gacaatatat atgtaagtgc cgtcaatggt
    31501 atcacacccg ggcaaattga gaatcattct gaatttcgcc aaacgtgcca ctgaacgttt
    31561 tctataatag aaaattcaac gtctgatgct gtacacagcg ccaacaatta ttggtgtcca
    31621 tgacgtattt gtggtataaa gcgcgccgga cttccgatcc atttcgtata cacagactgg
    31681 acggaagcga caatctcact ttgtgtaaca acacacacgt atcggcacat attccggggt
    31741 gccctttggg gtcggtaata tgggatacgt ggaggcataa ccccaacttt tatatagagg
    31801 ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa ggctggtgtt cacttcggtc
    31861 accagacccg ttactggaac ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag
    31921 ttcacatcat caaccttgag aaaactgtac cgatgttcaa cgaagctctg gctgaactga
    31981 acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg
    32041 aagcggtgaa agacgctgct ctgagctgcg accagttctt cgtgaaccat cgctggctgg
    32101 gcggtatgct gactaactgg aaaaccgttc gtcagtccat caaacgtctg aaagacctgg
    32161 aaactcagtc tcaggacggt actttcgaaa agctgaccaa gaaagaagcg ctgatgcgca
    32221 ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat caaagacatg ggcggtctgc
    32281 cggacgctct gtttgtaatc gatgctgacc acgaacacat tgctatcaaa gaagcaaaca
    32341 acctgggtat tccggtattt gctatcgttg ataccaactc tgatccggac ggtgttgact
    32401 tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg ggcgctgtag
    32461 ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc ccaggcggaa gaaagcttcg
    32521 tagaagctga gtaataaggc ttgataactc ccccaaaata gttcgagttg cagaaaggcg
    32581 gcaagctcga gaattcccgg gagcttacat cagtaagtga ccgggatgag cgagcgaaga
    32641 taacgcatct gcggcgcgaa atatgaaggg ggagagccct tatagaccag gtagtacacg
    32701 tttggttagg gggcctgcat atggccccct ttttcacttt tatatctgtg cggtttaatg
    32761 ccgggcagat cacatctccg aggattttag aatggctgaa attaccgcat ccctggtaaa
    32821 agagctgcgt gagcgtactg gcgcaggcat gatggattgc aaaaaagcac tgactgaagc
    32881 taacggcgac atcgagctgg caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc
    32941 gaaaaaagca ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta
    33001 cggcatcatt ctggaagtta actgccagac tgacttcgtt gcaaaagacg ctggtttcca
    33061 ggcgttcgca gacaaagttc tggacgcagc tgttgctggc aaaatcactg acgttgaagt
    33121 tctgaaagca cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat
    33181 caacattcgc cgcgttgctg cgctggaagg cgacgttctg ggttcttatc agcacggtgc
    33241 gcgtatcggc gttctggttg ctgctaaagg cgctgacgaa gagctggtta aacacatcgc
    33301 tatgcacgtt gctgcaagca agccagaatt catcaaacca gaagacgtat ccgctgaagt
    33361 ggtagaaaaa gaataccagg tacagctgga tatagcgatg cagtctggta agccgaaaga
    33421 aatcgcagag aaaatggttg aaggccgcat gaagaaattc accggcgaag tttctctgac
    33481 cggtcagccg ttcgttatgg aaccaagcaa aactgttggt cagctgctga aagagcataa
    33541 cgctgaagtg actggcttca tccgcttcga agtgggtgaa ggcatcgaga aagttgagac
    33601 tgactttgca gcagaagttg ctgcgatgtc caagcagtct taattatcaa aaaggagccg
    33661 cctgagggcg gcttcttttt gtgcccatct tgtaaattca gctaaccctt gtggggctgc
    33721 gctgaaaagc gacgtacaat gtcgctagta ttaattcatt tcaatcgttg acagtctcag
    33781 gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac gcattctgct taagttgagt
    33841 ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg caagcatact ggatcgtatg
    33901 gctcaggaaa tcaaagaact ggttgaactg ggtattcagg ttggtgtggt gattggtggg
    33961 ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac
    34021 cacatgggga tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc actgcaccgc
    34081 gcctatgtga acgctcgtct gatgtccgct attccattga atggcgtgtg cgacagctac
    34141 agctgggcag aagctatcag cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt
    34201 acaggtaacc cgttctttac caccgactca gcagcttgcc tgcgtggtat cgaaattgaa
    34261 gccgatgtgg tgctgaaagc aaccaaagtt gacggcgtgt ttaccgctga tccggcgaaa
    34321 gatccaaccg caaccatgta cgagcaactg acttacagcg aagtgctgga aaaagagctg
    34381 aaagtcatgg acctggcggc cttcacgctg gctcgtgacc ataaattacc gattcgtgtt
    34441 ttcaatatga acaaaccggg tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact
    34501 ttaatcacgg aataattccc gtgatggata aataagggta agattccgcg taagtatcgc
    34561 gggggcgtaa gtctggttat aaggcgttat tgttgcaggc agtttggtca cggccagcgc
    34621 gcagcaaccg gagcgtacaa aagtacgtga ggatggcgag cactgctcgg ggccaaaatg
    34681 gcaaataaaa tagcctaata atccagacga ttacccgtaa tatgtttaat cagggctata
    34741 cttagcacac ttccactgtg tgtgactgtc tggtctgact gagacaagtt ttcaaggatt
    34801 cgtaacgtga ttagcgatat cagaaaagat gctgaagtac gcatggacaa atgcgtagaa
    34861 gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg cttctcccag cctgctggat
    34921 ggcattgtcg tagaatatta cggcacgccg acgccgctgc gtcagctggc aagcgtaacg
    34981 gtagaagatt cccgtactct gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt
    35041 gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga actctgcggg tagcgacatc
    35101 cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt
    35161 ggtgaagcag aacaagcgcg tgttgcagta cgtaacgtgc gtcgtgacgc gaacgacaaa
    35221 gtgaaagcac tgttgaaaga taaagagatc agcgaagacg acgatcgccg ttctcaggac
    35281 gatgtacaga aactgactga tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa
    35341 gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc agtagatcct
    35401 tgcggatcgg ctggcggcgt tttgcttttt attctgtctc aactctggat gtttcatgaa
    35461 gcaactcacc attctgggct cgaccggctc gattggttgc agcacgctgg acgtggtgcg
    35521 ccataatccc gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat
    35581 ggtagaacag tgcctggaat tctctccccg ctatgccgta atggacgatg aagcgagtgc
    35641 gaaacttctt aaaacgatgc tacagcaaca gggtagccgc accgaagtct taagtgggca
    35701 acaagccgct tgcgatatgg cagcgcttga ggatgttgat caggtgatgg cagccattgt
    35761 tggcgctgct gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct
    35821 ggccaataaa gaatcactgg ttacctgcgg acgtctgttt atggacgccg taaagcagag
    35881 caaagcgcaa ttgttaccgg tcgatagcga acataacgcc atttttcaga gtttaccgca
    35941 acctatccag cataatctgg gatacgctga ccttgagcaa aatggcgtgg tgtccatttt
    36001 acttaccggg tctggtggcc ctttccgtga gacgccattg cgcgatttgg caacaatgac
    36061 gccggatcaa gcctgccgtc atccgaactg gtcgatgggg cgtaaaattt ctgtcgattc
    36121 ggctaccatg atgaacaaag gtctggaata cattgaagcg cgttggctat ttaacgccag
    36181 cgccagccag atggaagtgc tgattcaccc gcagtcagtg attcactcaa tggtgcgcta
    36241 tcaggacggc agtgttctgg cgcagctggg ggaaccggat atgcgtacgc caattgccca
    36301 caccatggca tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatt tttgcaaact
    36361 aagtgcgttg acatttgccg caccggatta tgatcgttat ccatgcctga aactggcgat
    36421 ggaggcgttc gaacaaggtc aggcagcgac gacagcattg aatgccgcaa acgaaatcac
    36481 cgttgctgct tttcttgcgc aacaaatccg ctttacggat atcgctgcgt tgaatttatc
    36541 ggtactggaa aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga
    36601 tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc
    36661 cggctacaga gagtctcgct atttgttagc gtagggcttc agtgatatag tctgcgccat
    36721 ctgatcgtaa gtagttggct ttataaggtc aggtatgccg tggttttgca cggctttttt
    36781 tgtataggct tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga
    36841 tgttgtctgc tactcaacta cttagcgaaa aattgccagc gcatggctgc cgtcatgttg
    36901 cgatcattat ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg
    36961 ggcataaagc cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta
    37021 ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag
    37081 tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc
    37141 gacataacgt gcgtctgcgt attattggcg ataccagtcg ctttaactcg cgtttgcaag
    37201 aatgtattcg taaatctgaa gcgctaacag ccgggaatac cggtctgacg ctgaatattg
    37261 cggcgaacta cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg
    37321 tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga gatgctaaac cagcatgtct
    37381 gtatgcatga actggcccct gtagatttag taattaggac tgggggggag catcgcatta
    37441 gtaacttttt gctttggcaa attgcctatg ccgaacttta ctttatagat gttctctggc
    37501 ccgatttcga tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc
    37561 gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg gggggcgctt ttgctgaagt
    37621 atcgcctgat atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc
    37681 cgccggtggg gttcgccatt gtaacgctgg tggtctgcat gctggcagcg tgggaatggg
    37741 gacagcttag cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt
    37801 tattgttggc gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc
    37861 tggttgaaat ctcactttgg gcttcgctgg gttggtggat tgtctcgcta ttgctggtgc
    37921 tgttttaccc aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg
    37981 gcgtgctaac cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactatg
    38041 acgagaatca ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggggcg
    38101 ctgactccgg cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg
    38161 tttctccggg taaaacctgg caaggcttta taggtggact cgctactgca gcggtaatct
    38221 catggggtta tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct
    38281 ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg
    38341 aagcaggaat taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta
    38401 ttgatagcct gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga
    38461 cgctttaacg gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc
    38521 actgggtgta cttatcactg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg
    38581 tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa
    38641 gctcggcacc gaatatgtta tcgccctgat cccgctgggc ggttatgtca aaatgctgga
    38701 tgagcgcgca gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt
    38761 tggccaacga gcggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt
    38821 tgcctactgg ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggtgaaat
    38881 agcagccaat tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt
    38941 agatggtatc gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg
    39001 cgatgaaagc accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa
    39061 gctcgattta cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg
    39121 gattcgtcct cgtgggctgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc
    39181 ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt
    39241 aacgcagtgg gtgacctttg tgatgcttgt ccgggataac ccggggaaat ccttagcgtt
    39301 agaaatcgaa aggcagggaa gtcccttgtc tttgacatta atcccggaga gtaaaccggg
    39361 taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga
    39421 gtataaagtt gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac
    39481 gtggcagctg atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa
    39541 actgaacaac ctcagtgggc cgatctctat cgccaagggg gctgggatga cagcggaact
    39601 cggggtagtt tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa
    39661 cctgtttccg ttgcccgtac ttgacggggc gcatctgctg ttccttgcga tcgaaaagat
    39721 caagggcgga ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct
    39781 gctggtgctg ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt
    39841 taggaagaac gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt
    39901 agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc
    39961 cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg
    40021 gttaatgatg aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag
    40081 gatgttcgcg tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc
    40141 attgccagca ttactttctc cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac
    40201 ctcgaggctt ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc
    40261 gagaaaggtc tggaagactt ctactacagc gtcggtaaat atagcgccag cgtaaaagct
    40321 gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg
    40381 tcagctgaaa tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg
    40441 atctctcatt tccaactgcg tgacgaagtg ccgtggtgga acgtggtagg cgatcgtaaa
    40501 taccagaaac agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggatcgc
    40561 ggttatgccc gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt
    40621 atttacgtca cggtgaacat taccgaaggc gatcagtaca agctttctgg cgttgaagtg
    40681 agcggcaacc ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc
    40741 gagctgtata acggcaccaa agtgaccaag atggaagatg acatcaaaaa gcttctcggt
    40801 cgctatggtt atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa
    40861 accgttaaat tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt
    40921 tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa
    40981 ggtgcatggc tgggaagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc
    41041 ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga ccaagtagat
    41101 gtagtctaca aggtgaaaga acgtaacacc ggtagcttca acttcgggat tggttacggt
    41161 actgaaagtg gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt
    41221 tatgctgttg gtatcaacgg gaccaaaaac gattaccaga cctatgctga attgtcggta
    41281 accaacccgt acttcactgt agatggtgta agcctcggtg gtcgtctctt ctataatgac
    41341 ttccaggcag atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg
    41401 acgttgggct tcccgatcaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat
    41461 aactccctgt ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt
    41521 gaacatccga gcacctctga tcaggataac agcttcaaaa cggacgactt cacgttcaac
    41581 tatggttgga cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc
    41641 aacctgaccg gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta
    41701 gacacggcga cttatgtgcc gatcgatgac gatcacaaat gggttgttct ggggcgtacc
    41761 cgctggggtt atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat
    41821 gccggtggtt ccagcaccgt ccgtggcttc cagtccaata ccattggtcc gaaagcagtt
    41881 tacttcccgc atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag
    41941 gacggcgcga aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt
    42001 gccagcctcg agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt
    42061 cgtacttcct tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa
    42121 tattctggat atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca
    42181 ttacaatgga tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag
    42241 tacgatggag acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc
    42301 tccacaaagg aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc
    42361 gggccacgca aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa
    42421 aaagtggtta ttagcggcag gtctcggttt agcactggca acttctgctc aggcggctga
    42481 caaaattgca atcgtcaata tgggcagcct gttccagcag gtagcgcaga aaaccggtgt
    42541 ttctaacacg ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac
    42601 cgatctgcag gctaaaatga aaaagctgca gtccatgaaa gcgggcagcg atcgcactaa
    42661 gctggaaaaa gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga
    42721 gcaggatcgc gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac
    42781 tgctgtgaaa tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgccgt
    42841 tgcttacaac agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata
    42901 agtaatgcct tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg
    42961 tgatggcgat atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat
    43021 tacgttcatg gttaacccaa aataccgtga gcatttaggc ttgtgccagg cgtccgcggt
    43081 tgtcatgacc caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc
    43141 ctacctgact tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa
    43201 cattgcaccc agtgcggtga tcgacgcgac ggcgaagctg gggaacaatg tatcgattgg
    43261 cgctaacgcg gtgattgaat ccggcgttga actgggcgat aacgtgatta tcggtgccgg
    43321 ttgcttcgta ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac
    43381 catttaccat gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg
    43441 cgcagacggc tttggttatg ccaacgatcg tggtaactgg gttaagatcc cacagattgg
    43501 tcgcgtaatt attggcgatc gcgtggagat cggtgcctgc actaccatcg atcgcggcgc
    43561 actggatgac actgttattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca
    43621 taacgtcgtg attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct
    43681 gaaaattggt cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat
    43741 atgcgacaaa gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg
    43801 cgtctattcc tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact
    43861 ggtgatgaac attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca
    43921 acaagactaa cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat
    43981 tgcggcaggc cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact
    44041 actaacactc atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg
    44101 ttcttactgg tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa
    44161 aatgtctctg tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg
    44221 ggtgtgctga ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta
    44281 ggaaaactgg aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag
    44341 cgcccggtcg tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc
    44401 ggcctgaccc gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg
    44461 atgatgtgtg ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat
    44521 ccaaccgcca ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt
    44581 tgtatcgttg gaccccatgt cggaattggt gagggtaccg tactgaaatc tcacgttgtc
    44641 gtgaatggtc atactaaaat tggccgcgat aatgagattt atcagttcgc ctccatcggc
    44701 gaagttaacc aggatctgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt
    44761 aaccgcattc gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg
    44821 aaggtgggca gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta
    44881 ggtaaccgct gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac
    44941 ttcgcgatca tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcacgtg
    45001 atggttggcg gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt
    45061 aaccacgcaa cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt
    45121 gaggcgatta ccgctatccg caatgcgtat aagctgattt atcgtagcgg taaaacgctc
    45181 gatgaagtga aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt
    45241 accgatttct tttcacgctc aacgcgcggt ctgattcgtt aatgactgaa cagcgtccat
    45301 taacgattgc cctggtcgcc ggagaaacct ccggcgatat cctgggggcc ggtttaatcc
    45361 gcgctctgaa agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc
    45421 aggctgaagg ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg
    45481 aagtgctcgg tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg
    45541 gcgaactgaa gccagatgtt tttgttggta ttgatgcgcc tgacttcaat attactcttg
    45601 aaggtaacct caaaaagcag ggtatcaaaa ccattcatta cgtcagtccg tcagtctggg
    45661 cgtggcgaca gaaacgtgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc
    45721 tgcctttcga aaaagcgttt tatgacaaat acaacgtacc gtgccgcttt atcggtcata
    45781 ccatggctga tgccatgcca ttagatccag ataaaaatgc cgcccgtgat gtgctgggga
    45841 tccctcacga tgcccactgc ctggcgttgc taccggggag ccgtggtgca gaagttgaaa
    45901 tgcttagtgc cgatttcctg aaaacggccc agcttttgcg ccagacatat ccggatctcg
    45961 aaatcgtggt gccactggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg
    46021 aagtcgcgcg agacttttca gttcatttgc tggatgggat gggccgtgag gcgatggtcg
    46081 ccagcgatgc ggcgctactg gcgtcgggta cggcagccct ggagtgtatg ctggcgaaat
    46141 gcccgatggt ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg
    46201 tgaaaactga ttatgtctcg ctgccaaatc tgctggcggg cagagagtta gtcaaagagt
    46261 tattgcagga agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga
    46321 acgggaaaat cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct
    46381 gcaatgccga tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt
    46441 ttatccgcac acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg
    46501 cgcggtcgtc accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga
    46561 ttccaaaaag ctgagcgaaa aacgccgtct ggcgctctgt gaagagatca aagagaaagc
    46621 gttgagctgg agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca
    46681 tgcgaccatg ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt
    46741 gttgattgat ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg
    46801 cgatagccgc gtaccggaaa tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc
    46861 cgaaatggcg gcgctggata ttgttttccc gcaatatggt tttgcccaac acaaagggta
    46921 cccaaccgct tttcatctgg aaaaactggc tgaacacggc gcgaccgaac accatcggcg
    46981 cagctttggg cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta
    47041 agtaaaccgg aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga
    47101 ctactcgatg atcgatggcc tggccaaaac tgcaccgttg gtaaaaaagg cggcggcgtt
    47161 gggtatgcca gcactggcga tcaccgattt caccaacctt tgtggtctgg tgaagttcta
    47221 cggagcggga catggcgcag ggattaagcc tatcgtcggg gcagatttta acgtccagtg
    47281 cgacctgctg ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta
    47341 tcagaatctg acgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgctgggcc
    47401 gatcatcgat cgcgactggc ttatcgaatt aaatgaaggg ttgatccttc tttccggcgg
    47461 gcgcatgggc gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg
    47521 tgtcgcgttt tatgaagaac acttcccgga tcgctatttt ctcgagctga tccgcaccgg
    47581 caggccggat gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt
    47641 gcccgtcgtg gcgactaacg atgtgcgctt tatcgacagc agcgactttg acgcacacga
    47701 aatccgcgtc gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta
    47761 ttcgccgcag caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc
    47821 cgaagccctt gccaacaccg ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg
    47881 tgaatacttc ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa
    47941 gcgtgcaaaa gagggcctgg aagagcgtct ggccttttta ttccctgacg aggaagaacg
    48001 tgttaagcgc cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca
    48061 gatgggcttc ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa
    48121 cggcgtaccg gtagggccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct
    48181 gaaaatcacc gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc
    48241 ggaacgtgtc tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt
    48301 tatcgagcac gtagcggaca tgtacggtcg tgatgcggta tcgcagatca tcaccttcgg
    48361 tacaatggcg gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg
    48421 ctttgtcgat cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc
    48481 gtttgaagcc gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct
    48541 gatcgacatg gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg
    48601 ggtggttatc gcgccgacca aaattaccga ttttgcgccg ctttactgcg atgaagaggg
    48661 caaacatccg gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt
    48721 cgacttcctt ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa
    48781 gcggcgggcg aagaatggcg agccgccgct ggatatcgcc gcgatcccgc tggatgacaa
    48841 gaaaagcttc gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg
    48901 cggcatgaag gatctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact
    48961 ggtggcgctg ttccgcccag gtccgttgca atcagggatg gtggataact ttatcgaccg
    49021 taaacatggt cgcgaagaaa tctcctatcc ggacgtacag tggcagcatg aaagcctgaa
    49081 accggtactg gagccaacct acggcatcat cctgtatcag gaacaggtca tgcagattgc
    49141 gcaggtgctt tctggttata ccctcggtgg cgcggatatg ctgcgtcgtg cgatgggtaa
    49201 gaaaaagccg gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa
    49261 cggagtcaac gccgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta
    49321 cggatttaac aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct
    49381 gaaagcgcac tatccggcgg agtttatggc ggcggtaatg accgccgata tggacaacac
    49441 cgagaaggtg gtgggcctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc
    49501 agatataaac tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg
    49561 tattggcgcc atcaaagggg taggtgaagg tccgattgag gccatcatcg aagcccgtaa
    49621 taaaggcggc tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt
    49681 aaaccggcgg gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca
    49741 ccgcgcggcg ctgatgaact cgctgggtga tgcgttaaaa gcggcagatc aacacgcgaa
    49801 agcggaagct atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat
    49861 tgaacaatcc tacgccagct gccaaccgtg gccggagcag gtggtgttag atggggaacg
    49921 tgaaacgtta ggtctgtacc ttacgggaca ccctatcaac cagtatttaa aagagattga
    49981 gcgttatgtc ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat
    50041 cacggctgcg gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat
    50101 cggtatctgc acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc
    50161 cctggataaa taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt
    50221 cagctttgat gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga
    50281 cgaagcccgg gaaaaatatg ctagcgggct tgctatctcg ctgacggaca ggcaaattga
    50341 tgaccagctt ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc
    50401 agtacatctc tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg
    50461 gcgtgtctct ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca
    50521 ggtggaactg gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga
    50581 acagccgatt gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga
    50641 tgagaaactg gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga
    50701 actgacacgt aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca
    50761 tccacagcgt ccttataccc tggattacgt tcgcctggca tttgatgaat ttgacgaact
    50821 ggctggcgac cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtcttga
    50881 cggtcgtccg gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg
    50941 ccgtaacttt ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat
    51001 ggctgaacgc tttaagatgc ctatcatcac ctttatcgac accccggggg cttatcctgg
    51061 cgtgggcgca gaagagcgtg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc
    51121 tcgcctcggc gtaccggtag tttgtacggt tatcggtgaa ggtggttctg gcggtgcgct
    51181 ggcgattggc gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc
    51241 gccggaaggt tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc tggcggctga
    51301 agcgatgggt atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc
    51361 ggaaccgctg ggcggcgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca
    51421 actgctggcg gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg
    51481 ttatcagcgc ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca
    51541 cttcggtggc ccttttttat cgccacggtt tgagcaggtt atgattaagg aaggattttc
    51601 caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat
    51661 gagcccatca aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca
    51721 caaaacagtg ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt
    51781 tttgactggg atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat
    51841 ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg
    51901 cggatggcgc tctggttttt tgaatatgcg ctggggcagg tggaagatat cgccattcgt
    51961 atgcgtcagt acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg
    52021 tttacctacg ttaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc
    52081 gcatatcaaa aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc
    52141 aaggctgatg tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca
    52201 cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt
    52261 accaacggaa catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt
    52321 acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat
    52381 gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg
    52441 caccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa
    52501 tggccggttc atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac
    52561 tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac
    52621 acccattttc atccgatcta ccaggggaaa agtgatcttc gaaacgcaat cgacccacaa
    52681 aatgctggcg gcgttatcgc aggcttcgct gatccacatt aaaggcgagt atgacgaaga
    52741 ggcgtttaac gaagccttta tgatgcatac caccacctcg cccagttatc ccattgttgc
    52801 ttcggttgag acggcggcgg cgacgctgcg tggtaatccg ggcaaacggc tgattaaccg
    52861 ttcggtggag cgggcgctgc attttcgcaa agaggtccag cggttgcggg aagagtctga
    52921 cggttggttt ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt
    52981 tgcgccaggc gaacagtggc acggctttaa cgatgcggat gccgatcata tgtttctcga
    53041 tccggttaaa gtcactattt tgataccggg gatggacgaa cagggcaata tgagcgagga
    53101 ggggatcctg gcggcgctgg tggcaaaatt cctcgacgaa cgtgggatcg tagtagagaa
    53161 aaccggccct tataacctgc tgtttctctt tagtattggt atcgataaaa ccaaagcaat
    53221 gggattattg cgtgggttga cggaatttaa acgctcttac gatctcaacc tgcggatcaa
    53281 aaatatgcta cccgatctct atgctgaaga tcccgatttc taccgcaata tgcgtattca
    53341 ggatctggcg caagggatcc ataagctgat tcgtaaacac gatcttcccg gtttgatgtt
    53401 gtgggcattc gatactttgc cggaaattat catgacgcca catcaggcat ggcagagaca
    53461 gattaaaggt gaagtagaaa ccattgcgct ggaacaactg gtcggtagag tatcggcaaa
    53521 tatgatcctg ccttatccac cgggcgtacc gctgttgatg ccaggagaaa tgctgaccaa
    53581 agagagccgc acggtactcg attttctact gatgctttgt tccgtagggc aacattaccc
    53641 cggttttgaa acggatattc acggcgcgaa acaggacgaa gacggcgttt accgcgtacg
    53701 agtcctaaaa atggcgggat aacttgccag agcggcttac gggcgagtaa cgtgctgtta
    53761 acaaataaag gagacgttat gctgggttta aaacaggttc accatattgc gattattgcg
    53821 acggattatg cggtgagcaa agctttctac tgcgatattc ttggtttcac gctgcaaagc
    53881 gaagtctatc gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat
    53941 gtgattgagc ttttctcttt tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc
    54001 ggcctgcgtc atctggcttt tagcgttgat gacatcgatg cggcagtggc gcaccttgaa
    54061 agccataacg tgaagtgtga agccatccgt gtcgatccat acacgcaaaa acgcttcacc
    54121 ttctttaacg atccggacgg gctgccgttg gaactgtatg agcagtaagg cttgtcatcg
    54181 ccgcatttgc ccggtaacgt gccgggcatt gctactgtaa aatcgcacca tcatgacact
    54241 cacgctcaat agacaacttc tcacctcacg ccagattttg gtcgccttta gcggcgggct
    54301 tgactccacc gttctgctgc atcagttggt gcagtggcgg acggaaaatc cgggcgtcac
    54361 tctgcgcgct attcatgtgc atcacggttt aagtgccaat gccgatgcct gggttacgca
    54421 ttgcgaaaac gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg tacaacttgc
    54481 gcaagagggg ctggggattg aggcccaggc gcggcaggcg cgttatcagg catttgcccg
    54541 taccctattg cccggcgaag tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac
    54601 ctttctgctg gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta tggcggaagt
    54661 ttcggagttt gccggaacac ggcttatccg cccgttgctt gcccgcacgc ggggggaact
    54721 ggcgcagtgg gcattggcgc atggtttacg ctggattgaa gacgaaagta atcaggacga
    54781 ctcatacgat cgtaactttc tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc
    54841 gcattttgcg gaagcaacgg cccgcagcgc cgcactttgt gctgaacaag agagtctgtt
    54901 ggatgaactg ctggcggatg atttagcaca ctgccaaacg tcgcagggga cgctgcagat
    54961 tgcgccaatg ctggcgatga gtgatgcccg ccgcgcggcg attatccgtc gctggctggc
    55021 agggcagaat gcaccaatgc cttcccgcga cgcgctggtg cggatctggc aggaagtggc
    55081 gctggcgcgg gaagatgcct ccccctgttt acgcttgggc gcgtttgaaa tccgccgcta
    55141 tcagtcgcaa ctgtggtgga ttaaatccgt caccgggcaa agtgaaacca ttgtgccgtg
    55201 gcagacgtgg ctacaaccgc tggaattacc ggcggggctg ggaagcgtac agcttactgc
    55261 gggaggcgat attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaatcggc
    55321 aggattgctg catattgtcg ggcgcaacgg cggacgtaag ctgaagaaaa tctggcaaga
    55381 gttaggcgtg ccgccgtggc tacgtgacac cacgccactg ctgttttatg gcgaaacgct
    55441 gattgcggcg gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg agaatggcgt
    55501 aagttttgtc tggcagaaaa cgcttagtta agtgaaagcc ggataagacg catcaaacgt
    55561 cgcatccggc gaaagtcaat caggactcgc tcaccaccac cgtaccgatt tccgggtggc
    55621 taaagctggt aattttatcc agacgtagct cacgggtttc gccagcggcc tcgacgacca
    55681 gatactccac atttttgcgg gagactaaat cgctggcttt tgcctgcaat ttttcgccat
    55741 ctttcagctc aagtgtcagc attaaatgat gctggcaggc gagctcaaga ttatcgtaat
    55801 catcacaatt gattggttga tacgtatcat tcattaacat aatcgctcac cagtaagttt
    55861 gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc ggcagccatt
    55921 tcattcagca ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa gtctccgctg
    55981 gcgatttccg cgtaccgctt gcgtattaac tcacaatatt tttccacatg ccctcctgtc
    56041 agcacactga cttaaccgtg gatgcaagtc taagcctacg aagataaact ctgtttcgca
    56101 aggtgactat accacactca tttctgcaat atcagcgccg caactgcacg tattccgtta
    56161 caatggcctc ctgattcgaa aggagttttc ttatggcgct taaagcgaca atttataaag
    56221 cgacggttaa tgtggccgat ctcgaccgca accagtttct cgatgcctct ctgacgctgg
    56281 cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg cctgctggcg tggctgaaat
    56341 atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag ccggaagcgt
    56401 ggctgcgtaa cgatcacctg ggcattgatt tgtggattga gctggggctg ccggatgagc
    56461 ggcggattaa gaaagcctgc acccaggccg cagaagtggc gctgtttacc tataatagtc
    56521 gggcggcgca aatctggtgg cagcaaaatc agagcaaatg tgtgcagttt gccaatcttt
    56581 ccgtctggta tctggacgat gaacaactgg cgaaagtaag cgcctttgcc gatcgtacca
    56641 tgacgctgca ggcaacgatt caggatggcg tgatctggtt atcggacgat aagaataatc
    56701 tggaagttaa cttaaccgcc tggcaacaac cttcatgatt gtgatttccc gacatgttgc
    56761 tattcccgat ggtgagcttg agatcaccgc cattcgtgcg cagggcgcgg gcgggcagca
    56821 tgttaataag acctcaacgg ccattcatct gcgttttgac attcgggcgt ccagcctgcc
    56881 agagtattac aaagagcgtc tgctcgccgc cagccatcat ttgatcagca gtgatggcgt
    56941 gattgtcatt aaggcacagg aataccgcag tcaggaactg aaccgcgaag ccgcgctagc
    57001 ccggctggtg gcagtgatta aagatttaac aacagaacaa aaagcccgac gacccacgcg
    57061 gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa gcacaaaaat caagcgcgaa
    57121 ggcgatgcgc ggcaaagtac gcagcggtcg ggaataaaaa gaaggaatag atggtgaaaa
    57181 aagcgatagt gacagcgatg gctgtaatca gcctctttac tctgatggga tgtaataacc
    57241 gggccgaagt cgatacgctt tctccagcac aggctgccga actgaaaccg atgccgcaaa
    57301 gttggcgcgg cgtgctgccg tgtgctgatt gtgaaggaat cgaaacctct ctgttcctcg
    57361 aaaaagacgg aacatgggtg atgaatgagc gttatctcgg tgctcgtgaa gaaccttcct
    57421 ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta accgacagca
    57481 aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct ggagatgctc gatcgtgaag
    57541 gcaatccgat tgaatcgcag ttcaactata cgctggaacc ggcacaatcc agtttaccta
    57601 tgacgccgat gaccctgcga ggcatgtatt tttatatggc tgatgcggcg accttcactg
    57661 attgcgcgac cggaaaacgt ttcatggtag cgaataacgc agagctggag cgtggctacc
    57721 tggctgcgcg cggtcacagt gaaaaaccgg tgttactgtc agtagaaggt cactttacgc
    57781 ttgaggctaa tccggatact ggtgcgccaa ccaaagtatt agcgcccgat acggcgggta
    57841 aattttaccc gaataaggat tgcagtagtt tggggcagta aacctgctta aggcagaaac
    57901 aaacgcaaaa caggccagaa ggatatattt caacattttg aatttgcacg ttttttgtag
    57961 gccggataag gcgtttacgc cgcatccggc aatggtgctc aacgcctgat gcgacgctgg
    58021 cgcgtcttat catgcctaca acccccctca taccttaagg cggataaggc atttacttta
    58081 tcaccgggtc aaaccaacaa agcgacccgc cttgatataa atccctggct tcaggtgccc
    58141 aacatactgt aactgcgttt ctggcttaaa cgcagagaca tcaccaatac gcatatgcat
    58201 cagatcggca gggctaatcc agcccgattg cgccagtgta agtggatgtc cggctttggc
    58261 aaatgcatca gtgacaaatt ccgaacaaaa ccacgacttt ttgtctcctt cgcccacact
    58321 gcttaactgc gctttcgcca ggccgctgac gcactgctgg cgaaaatcct cggagaactg
    58381 attcagtgag cacatctggc gagtcaccat aaagggaata aattcgacaa tgccgcgata
    58441 gttataaccg ctatctttga ttttattggc aaacgcggtg atatctgtgg cttgttgcgg
    58501 ggtaagatcc gggactcgta agacgaaaag cttatcacta tgcttcatcg cttttttaag
    58561 ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg ttattatcac ccagaaagat
    58621 tgcaacgtga ctcacagagg aagtgctgaa gacgcggatt ccaaatgagg ttaccccaag
    58681 gcttgaggag aacagcaaat cgccgggttt gagatcaggt gccgttattt cttttattga
    58741 ttgttcggtg aaagagcttt gatgctggaa tttgacagcc catgtttttg cctcggcatc
    58801 tactgctgtg gctgatgggg ctggctgata tcaacggtac aagccgaaag taaaagaaag
    58861 ctggggagaa gcaggcggca gtacgccttt ggtttatcca ttttatacaa tccatgtaaa
    58921 aaagggccct gaaattcagg accctttctg gcatcagcct ttaatctgtt tcaccagata
    58981 ttcgacgatg tcaccagtct taattaactg tttctcgccg ttgcgacgat atttatattc
    59041 gatatcgtcg ttgtcgaggt tacggtcgcc cagcacaata gtgtgcggaa taccgatcag
    59101 ttccatatca gcaaacatca cgcccggacg ctctttgcgg tcatccagca gcacttcgat
    59161 accttgtgca cgcagttcgc tgtacagttt ctcagcaagc tcttgtacgc ggaaggattt
    59221 gtgcatgttc atcggcagaa tcgccacctg gaacggcgcg atagcgtcag gccatacgat
    59281 gccgcgttcg tcgtagttct gctcaatcgc cgcagctacc acacgcgtta ccccgatacc
    59341 gtagcaaccc atcgtcagga tttggttacg gccatcttca ccctgtacgg aggctttcag
    59401 tgcttcggag tacttggtac ccagctggaa gatgtgacca acttcgatac cacgtttgat
    59461 cagcagcgta ccctggccat ccgggcttgg atcgccagcc accacgttac ggatatctgc
    59521 aacttccggg gtagcgacat cgcgatccca gttgataccg aagtagtgtt taccatcgat
    59581 gttagcacca gcagcgaaat cactcatcgc cgcaacggta cggtcaatca ccaccggaat
    59641 cggcatgttt accggaccca gtgaacccgg accggcttta accacagcac gaatttcttc
    59701 ttcggtcgcg aaagtcagcg ggcttgcaac ctgcggcagt ttttctgctt taacttcgtt
    59761 cagctcgtga tcaccgcgca ccagcagcgc aaccagcggg aagctgctgc cttcaaccgc
    59821 tttaaccagc agagtcttaa ccgttttctc aatcggcaga ttgaactgtt caaccagttc
    59881 cgcgatggtt ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag cagcagcgcg
    59941 cggttctttc ggcgcgatag cttctgccag ttcaatgttc gctgcatagt cagaggtgtc
    60001 ggagaagacc acatcgtctt caccgctctg cgccagcacc tggaattcgt gagaggcgct
    60061 gccgccgata gaaccggtgt cggcttgcac ggcgcggaaa tccagcccca tgcggctgaa
    60121 gattttgctg taggccgcat acattgcatc gtaggtttcc tgcagggatt cctgagaagt
    60181 atggaaagag taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc cgaaacgcgg
    60241 acgcacttcg tcgcggaact tggtctggat ctgatagaag ttcagcggca gctgtttgta
    60301 agagctaagc tcgttacgaa tcaggtcagt gataacttct tcatgagttg ggccgagtac
    60361 gaacggacgc tcgccacggt caacaaaacg cagcagttcc ggaccgtact gttcccaacg
    60421 accactctct tgccacaaat cggctggctg aaccaccggc atcgacacct cgattgcacc
    60481 ggcgttgttc atctcttcac gcacgatgtt ttcgactttt ttcagaacgc gcacgccggt
    60541 cggcagccag gtatataacc cggaggccag cttgcggatc atcccggcgc gcagcatcag
    60601 ctgatggctg atcacctcgg cgtcggcagg tgtctccttg agagtggaga gcaggtattg
    60661 gctagtacgc atgttgttac ggttccagtt ggaaggtaga acaggctcaa ggcgagcctg
    60721 ggacaaaaaa agtgatttag tttaccagtg caaaagaaat gtcaaaagag aagggcgtga
    60781 atttaacgcg gttccagcgc aaagacttca aaacctgcgt cggtgacgcg ccagcgaacg
    60841 ttaaaatcat gtagccagac ggcataggtt ttgcccgttt cctcaccttt atgataggcc
    60901 gggcgcgggt cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg ataacgcttc
    60961 tccagcgtca aaagctgctt ttcgacctct gcggtaaaac tcaccgccat ctctgcagct
    61021 ggcgcacttt gcgcatagct ggcactggca tcgggaagcg attcggcaaa ggggagatac
    61081 ggtttgatat ccactaccgg cgtaccatcg accagatcca gactgccgag cttcagaatc
    61141 acgctgtctt tatggcaaac aacctctttc agctctacca gcgacatacc aattgggtta
    61201 gggcggaaag tagagcgtgt tgcgaaaacc cccattctgg cgttaccgcc gaggcgcggt
    61261 ggacgcacag tcggacgcca gccgccttcc atcgtttgat gaaagacgaa aaggatccat
    61321 aaatggctga acgcttccag gccgcgaacg gcgtcggcct ggttgtaggg agcaatgaga
    61381 tgcagttcgc cgttggcgct ttttaccaga cctggctggc gcggaacggc gaatttttct
    61441 ttatagggcg agcgaataac gcctatttgc tcgaactgga aactgctcat ttcgccgtaa
    61501 tgttaagcgc agagccgata catacagcct gacgatagca gcctggcgta ccgctggtga
    61561 cttcgcagct atgcagtaat accgcattgg ctttcatttt agaggcgttg atttgcatcc
    61621 gcttacgcgc ggttggaatg ctcggcggag agtcctgatt agaggcctgg caagagtcgc
    61681 cactgacttc accgagatcg cggaacggtt tgccgactaa ttcttctgca ttggtataaa
    61741 ttcggaccgg cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc ggctggggtg
    61801 cagtgctttg aacgggttcg acaggggatc tgcttaacat ggaacagccg cttagcatga
    61861 gtgctactaa acagatcggt aaagcacgca tagtatttcc tcaatgtatg atcaaaacgt
    61921 caatattgaa tcaggagctt gtaaaaatga caagacgggc aagtgcccat cctgaatgat
    61981 attacaaatt gtggaaacag cctaaaaatt accagccttt aacagcgccg ccgttaaaca
    62041 ctttgtttgc tgcttcgtaa acttcatcag actgataagc ctggacgaat ttcttcacgt
    62101 tctcggcgtc tttgttatct tcacgcgtta cgatcaggtt tacgtacggg gagtctttat
    62161 cttcgacaaa gataccgtct ttcgctggag tcaggccaat ctggctggca taggtggtat
    62221 tgataactgc cagagcgatt tgtgcgtcgt ccagagatcg aggcagctgc ggtgcttcca
    62281 gctcaacaat tttcagattt tttgggttct caacaacatc aagtacggtc ggcagcaggc
    62341 caacgccatc tttcagtttg atcaagccca ctttttgcag cagcagcagt gaacgaccaa
    62401 ggttagttgg atcgtttggc acggcaacct gcgaaccatc ctgcagttca tccagtgatt
    62461 tgattttctt ggagtaacca gcaatcggat aaacaaaagt gttgcctact gcgaccagtt
    62521 tgtagccacg atctttcagt tgctgatcaa ggtacggttt atgctggaag gcgttggcgt
    62581 cgatatcgcc tttgctcaat gcttcgtttg gcagaacata gtcgttgaag gttaccagct
    62641 caacgtccag gccatatttg tctttcgcaa ctttctgcgc gacttctgca acctgctgtt
    62701 cggcaccaac aatcacgccg actttaatgt ggtttggatc tttttcatcc tgaccgcagc
    62761 ctaccagtgc cagtgatcca atcagggctc ccactgccgc aaaggttttg aatttgaacg
    62821 ccatacctta ttccttcttc aattatttat gttgtgttga acgttacttg cgagtgacag
    62881 cccggacgat gcggtcgcct gcgaactgaa ttaaataaac cagaatgacc agcaatacca
    62941 gtaccgtatt catcaccgtc gcgttatagc cgatgtagcc atactgatag ccaatctgac
    63001 ctaaaccacc ggcaccgaca gcaccaccca tcgcggaata accgactagg gtaatcaggg
    63061 tgatagttgc cgcattcacc agacccggca atgcttccgg taacagcact ttacggacga
    63121 tctgcatcgg cgtggcaccc attgcacggg aagcttcaat taacccggtt gggatctcca
    63181 gcagagcgtt ctcgaccata cgggcaataa acggtgctgc accaacggtt aacggaacaa
    63241 tcgctgcctg caaaccaatc gatgtaccaa caataacgcg ggtaaacgga atcatccata
    63301 ccagcaagat aatgaacggg atagaacgga aaatgttcac aatcgcagaa acggtacgat
    63361 acagcttcgc gttagcaata atttgccccg gacgcgtgac ataaagcaga acgccaaccg
    63421 gcagaccaat cacaaagcca aaaaaaccgg atacgaaggt cattgccagc gtttcccata
    63481 cgccacgaac cagcagccac atcatcggct cagacataac ccagtacctc tacttttaca
    63541 tggtgttcct gcagccaggc aatggcggct tgcgtatctt gttgtgtgcc gtgcatttca
    63601 gtcagcatga tgccgaactt aacgccaccg gcgtagtcca tctgcgcgct aataatgttg
    63661 ttgttgacgt tgaaacgacg cgcggtttca gaaagcagtg gggcatcgac cgattgaccg
    63721 gtaaactcca gacgcagcat cggcacgcag tcagtaaatg gctccgcttg cagacgttcc
    63781 tggtaatctt ccgggatatc cagatgcagg gtcgactgaa taaacttctg cgccagcggc
    63841 gttttcggat gcgagaacac ttcacttacc gtgtcctgct cgatcagttc gccattgctg
    63901 atgaccgcca cgcaatcaca aatacgcttc acaacgtcca tttcgtgggt gatcaacaga
    63961 atcgtcaaac ccagacggcg gttaatgtct ttcagcagtt cgagaataga acgtgtcgtt
    64021 gccgggtcca gcgcgctggt ggcttcatca cacagcaata ctttgggatt gctggctaat
    64081 gcacgggcaa tcgccacacg ttgtttctgc ccaccggaaa gattcgacgg gtagctatca
    64141 tgcttatcgc caagaccaac caatgacagc aattccgtca cgcgacgttt gatctcgtct
    64201 ttcggtgtgt tgtccagctc cagcggcaga gccacgttgc caaaaacagt acgcgaagag
    64261 agcaggttaa aatgctggaa aatcatacca atctggcggc gagctttggt caactcggat
    64321 tctgacagcg tggtcagttc ctggccatcg accagcacgc taccctcggt tgggcgctcc
    64381 agcaggttta cacaacgtat aagcgtactc ttacccgcgc ctgaggcacc gataacgcca
    64441 taaatttgtc cagctggcac atgcaggctg acgttgttca acgcctggat ggtgcgggtg
    64501 ccctggtgga acactttggt gatattcgaa agttttatca ttgattattt attatcgtca
    64561 ttaagttagt cgtggcatct cgaatgcctg aaacgggcaa cgccgtcaat gaaatggatg
    64621 ttaaggcatc cagacgtcta aatcaatcag gtttatgcga agagcacttt cttgcaggtc
    64681 gaaacatgcg atactagcgt cacatgcctt attaaggagc tataaaaggt ggcaaagagc
    64741 gtacccgcaa tttttcttga ccgtgatggc accattaatg tcgatcacgg ctatgtccat
    64801 gagatcgaca actttgaatt tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa
    64861 atgggctttg tgctggtggt agtaaccaac cagtctggca ttgctcgcgg taaatttacc
    64921 gaagcacagt ttgaaacgct gaccgagtgg atggactggt cgctggcgga ccgagatgtc
    64981 gatctggatg gtatctatta ttgcccgcat catccgcagg gtagtgttga agagtttcgc
    65041 caggtctgcg actgccgtaa accacatccg gggatgcttt tgtcagcacg cgattatttg
    65101 catattgata tggccgcttc ttatatggtg ggcgataaat tagaagatat gcaggcagcg
    65161 gctgcggcga acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctat tacgcctgaa
    65221 gcagaaaacg cggcggattg ggtgttaaat agcctggcag acctgccgca agcgataaaa
    65281 aagcagcaaa aaccggcata atgattaaaa gatgagcgat tgaaataaaa atgcattttt
    65341 ccacttgtct tcctgagacg actccctata atgcgcctcc atcgacacgg cggatgtgaa
    65401 tc
//
LOCUS       DAJTYD010000005        60906 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTYD010000005 DAJTYD010000000
VERSION     DAJTYD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.005"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..322
                     /locus_tag="OO585_000297"
     CDS             <1..322
                     /locus_tag="OO585_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR6628002.1"
                     /translation="GTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAVELVVRGR
                     HKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVDLMEAHRN
                     ALGL"
     gene            377..679
                     /locus_tag="OO585_000298"
     CDS             377..679
                     /locus_tag="OO585_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6628003.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            694..813
                     /locus_tag="OO585_000299"
     CDS             694..813
                     /locus_tag="OO585_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR6628004.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            806..3883
                     /locus_tag="OO585_000300"
     CDS             806..3883
                     /locus_tag="OO585_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6628005.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            3880..4365
                     /locus_tag="OO585_000301"
     CDS             3880..4365
                     /locus_tag="OO585_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6628006.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            4362..5462
                     /locus_tag="OO585_000302"
     CDS             4362..5462
                     /locus_tag="OO585_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR6628007.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            5553..5771
                     /locus_tag="OO585_000303"
     CDS             5553..5771
                     /locus_tag="OO585_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR6628008.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(6006..7691)
                     /locus_tag="OO585_000304"
     CDS             complement(6006..7691)
                     /locus_tag="OO585_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6628009.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRI
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            7961..8338
                     /locus_tag="OO585_000305"
     CDS             7961..8338
                     /locus_tag="OO585_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6628010.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8368..8625)
                     /gene="grxA"
                     /locus_tag="OO585_000306"
     CDS             complement(8368..8625)
                     /gene="grxA"
                     /locus_tag="OO585_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6628011.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8785..9072
                     /locus_tag="OO585_000307"
     CDS             8785..9072
                     /locus_tag="OO585_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6628012.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9056..9778
                     /gene="nfsA"
                     /locus_tag="OO585_000308"
     CDS             9056..9778
                     /gene="nfsA"
                     /locus_tag="OO585_000308"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6628013.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9839..10741
                     /gene="rimK"
                     /locus_tag="OO585_000309"
     CDS             9839..10741
                     /gene="rimK"
                     /locus_tag="OO585_000309"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6628014.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            10829..11305
                     /locus_tag="OO585_000310"
     CDS             10829..11305
                     /locus_tag="OO585_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6628015.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11656..12768
                     /gene="potF"
                     /locus_tag="OO585_000311"
     CDS             11656..12768
                     /gene="potF"
                     /locus_tag="OO585_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6628016.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12863..13996
                     /gene="potG"
                     /locus_tag="OO585_000312"
     CDS             12863..13996
                     /gene="potG"
                     /locus_tag="OO585_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6628017.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14006..14959
                     /gene="potH"
                     /locus_tag="OO585_000313"
     CDS             14006..14959
                     /gene="potH"
                     /locus_tag="OO585_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6628018.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14956..15801
                     /gene="potI"
                     /locus_tag="OO585_000314"
     CDS             14956..15801
                     /gene="potI"
                     /locus_tag="OO585_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6628019.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            15861..16349
                     /locus_tag="OO585_000315"
     CDS             15861..16349
                     /locus_tag="OO585_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6628020.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16390..17547
                     /gene="rlmC"
                     /locus_tag="OO585_000316"
     CDS             16390..17547
                     /gene="rlmC"
                     /locus_tag="OO585_000316"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6628021.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQSEAAGAASDC"
     gene            complement(17691..18422)
                     /gene="artJ"
                     /locus_tag="OO585_000317"
     CDS             complement(17691..18422)
                     /gene="artJ"
                     /locus_tag="OO585_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6628022.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18713..19381)
                     /gene="artM"
                     /locus_tag="OO585_000318"
     CDS             complement(18713..19381)
                     /gene="artM"
                     /locus_tag="OO585_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6628023.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19381..20097)
                     /gene="artQ"
                     /locus_tag="OO585_000319"
     CDS             complement(19381..20097)
                     /gene="artQ"
                     /locus_tag="OO585_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6628024.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20104..20835)
                     /gene="artJ"
                     /locus_tag="OO585_000320"
     CDS             complement(20104..20835)
                     /gene="artJ"
                     /locus_tag="OO585_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6628025.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYNSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20853..21581)
                     /gene="artP"
                     /locus_tag="OO585_000321"
     CDS             complement(20853..21581)
                     /gene="artP"
                     /locus_tag="OO585_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6628026.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21799..22314)
                     /locus_tag="OO585_000322"
     CDS             complement(21799..22314)
                     /locus_tag="OO585_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6628027.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            22440..22763
                     /locus_tag="OO585_000323"
     CDS             22440..22763
                     /locus_tag="OO585_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6628028.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            22760..23590
                     /gene="amiD"
                     /locus_tag="OO585_000324"
     CDS             22760..23590
                     /gene="amiD"
                     /locus_tag="OO585_000324"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6628029.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(23587..24597)
                     /locus_tag="OO585_000325"
     CDS             complement(23587..24597)
                     /locus_tag="OO585_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6628030.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(24696..26126)
                     /locus_tag="OO585_000326"
     CDS             complement(24696..26126)
                     /locus_tag="OO585_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6628031.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(26137..27138)
                     /gene="ltaE"
                     /locus_tag="OO585_000327"
     CDS             complement(26137..27138)
                     /gene="ltaE"
                     /locus_tag="OO585_000327"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6628032.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(27175..28893)
                     /gene="poxB"
                     /locus_tag="OO585_000328"
     CDS             complement(27175..28893)
                     /gene="poxB"
                     /locus_tag="OO585_000328"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6628033.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(29026..29994)
                     /gene="hcr"
                     /locus_tag="OO585_000329"
     CDS             complement(29026..29994)
                     /gene="hcr"
                     /locus_tag="OO585_000329"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6628034.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(30006..31658)
                     /gene="hcp"
                     /locus_tag="OO585_000330"
                     /gene_synonym="priS"
     CDS             complement(30006..31658)
                     /gene="hcp"
                     /locus_tag="OO585_000330"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6628035.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLADKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(31802..32701)
                     /gene="lysO"
                     /locus_tag="OO585_000331"
     CDS             complement(31802..32701)
                     /gene="lysO"
                     /locus_tag="OO585_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6628036.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(33195..33890)
                     /gene="aqpZ"
                     /locus_tag="OO585_000332"
     CDS             complement(33195..33890)
                     /gene="aqpZ"
                     /locus_tag="OO585_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR6628037.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            34316..35974
                     /locus_tag="OO585_000333"
     CDS             34316..35974
                     /locus_tag="OO585_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6628038.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(35971..36927)
                     /locus_tag="OO585_000334"
     CDS             complement(35971..36927)
                     /locus_tag="OO585_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCR6628039.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            37078..38193
                     /gene="macA"
                     /locus_tag="OO585_000335"
     CDS             37078..38193
                     /gene="macA"
                     /locus_tag="OO585_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6628040.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            38190..40136
                     /gene="macB"
                     /locus_tag="OO585_000336"
     CDS             38190..40136
                     /gene="macB"
                     /locus_tag="OO585_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6628041.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(40209..40433)
                     /gene="cspD"
                     /locus_tag="OO585_000337"
     CDS             complement(40209..40433)
                     /gene="cspD"
                     /locus_tag="OO585_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6628042.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            40756..41076
                     /gene="clpS"
                     /locus_tag="OO585_000338"
     CDS             40756..41076
                     /gene="clpS"
                     /locus_tag="OO585_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6628043.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            41107..43383
                     /gene="clpA"
                     /locus_tag="OO585_000339"
     CDS             41107..43383
                     /gene="clpA"
                     /locus_tag="OO585_000339"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6628044.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(43727..43814)
                     /locus_tag="OO585_000340"
     tRNA            complement(43727..43814)
                     /locus_tag="OO585_000340"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43778..43780),aa:Ser,seq:gga)
     gene            complement(44068..44286)
                     /gene="infA"
                     /locus_tag="OO585_000341"
     CDS             complement(44068..44286)
                     /gene="infA"
                     /locus_tag="OO585_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6628045.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(44571..45275)
                     /gene="aat"
                     /locus_tag="OO585_000342"
     CDS             complement(44571..45275)
                     /gene="aat"
                     /locus_tag="OO585_000342"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6628046.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(45317..47038)
                     /gene="cydC"
                     /locus_tag="OO585_000343"
     CDS             complement(45317..47038)
                     /gene="cydC"
                     /locus_tag="OO585_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6628047.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(47039..48805)
                     /gene="cydD"
                     /locus_tag="OO585_000344"
     CDS             complement(47039..48805)
                     /gene="cydD"
                     /locus_tag="OO585_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6628048.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(48928..49893)
                     /gene="trxB"
                     /locus_tag="OO585_000345"
     CDS             complement(48928..49893)
                     /gene="trxB"
                     /locus_tag="OO585_000345"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6628049.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            50438..50932
                     /gene="lrp"
                     /locus_tag="OO585_000346"
     CDS             50438..50932
                     /gene="lrp"
                     /locus_tag="OO585_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6628050.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            51067..55017
                     /gene="ftsK"
                     /locus_tag="OO585_000347"
     CDS             51067..55017
                     /gene="ftsK"
                     /locus_tag="OO585_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6628051.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQQVAPQPQYQQPQQPV
                     APQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVE
                     ARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEV
                     IPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMP
                     HLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVV
                     TDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGD
                     SMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRP
                     SVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRV
                     HGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQF
                     VTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            55172..55783
                     /gene="lolA"
                     /locus_tag="OO585_000348"
     CDS             55172..55783
                     /gene="lolA"
                     /locus_tag="OO585_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6628052.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            55794..57137
                     /gene="rarA"
                     /locus_tag="OO585_000349"
                     /gene_synonym="mgsA"
     CDS             55794..57137
                     /gene="rarA"
                     /locus_tag="OO585_000349"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6628053.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            57228..58520
                     /gene="serS"
                     /locus_tag="OO585_000350"
     CDS             57228..58520
                     /gene="serS"
                     /locus_tag="OO585_000350"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6628054.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            58759..60906
                     /gene="dmsA"
                     /locus_tag="OO585_000351"
                     /pseudo
     CDS             58759..60906
                     /gene="dmsA"
                     /locus_tag="OO585_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
BASE COUNT        14920 a        15305 c        16430 g        14251 t
ORIGIN      
        1 tggtactgaa ctgctgctgt ttaagcagat gggcaaagcc acggtggatg gcatccagct
       61 gcgctttacc ggctctattc agcgtgatga taccggggaa gtgcaggccg tggagcttgt
      121 ggtgcgtgga cgtcacaaag aagtagattc cggcgagtgg aagacgggcg aaagcaacac
      181 taccaaagtg accagtacca acagctacgc gaagctgacc atcaatggtg aggtgctcta
      241 tgaagtggac ctgatcaaca tggtggaaat tgtggacggt gtggacctga tggaagcgca
      301 ccgcaacgcc ctcggcctct gatgtatctg aacggcgcgg aatgccgcgc cagaacctaa
      361 tttacaggac aacaaaatga gcgataagca gactgaaaag accattcaac tggatactcc
      421 catcatgcgc ggtaaaacag aaattaccga aattgtgttg cgtaaaccgc agtccggtgc
      481 gctgcgcggc acacgcctgc aggccattat ggatatggat gtgaacgcga tgatgaccgt
      541 gatcccccgc atctccagtc cggcactgac tgcacaggaa attgcagaga tggacccggc
      601 agatctcact gccatgtcgg ttgaggttgt cacttttttg ttgaagaagt cggtgcttgc
      661 cggtttaccg acagcctgac ggttgacgat ctggtggctg atatcgccac catctttcac
      721 tggccgccat ccgttactga cgttatgccg ctgaccgaag tgctggaatg gcgggataaa
      781 gcgattcaga gaagcggggc cagccatgag tgacaataac ctgcgtctgc aggtgattct
      841 taatgcggtt gacaaactca cccgcccatt tcgatccgcg caggccagtt caagagaact
      901 ggctgctgct gtcaaaaaat cccgcgatgc aataaagcag cttgatcagg ccgggagcag
      961 tctggacagc ttccgaaagc tgcaggcaga aaatcagaaa ttaggcgaca ggctgaacta
     1021 tgcccgccag cgtgcaaatt tgctcagtca ggaactggga gcgatggggc cgccttcgca
     1081 acgtcaggtt gttgctctgg gccgtcaacg gctggctgtt cagcgcctag aagaacgcca
     1141 gaaaaagctg cagcagcaga cggcgcttgt gcgtgcagaa ctttatcgtg ctggtatttc
     1201 agctaatgat ggcgccagtg cgacggcccg cattacccgt gaaacgatgc gttataacag
     1261 gcagctttct gagcaggaag cgaggttacg acgtgtcggg gagcaacagc gaaaaatgca
     1321 cgccgcccgg ggggcttacg ccaggcgtct tgaggtaagg gatcgtattg caggtgccgg
     1381 agccaccacc acggctgcag ggctggcaat gggcgcacca gtgatggcgg cagtaaaaag
     1441 ctataccagc atggaagatg ccatgaaagg tgtggcaaag caggtcaatg gtctgcgtga
     1501 cgataatggc aaccgcactg cgcgttttta cgaaatgcag ggtgccatca aggctgccag
     1561 cgaacagttg ccgatggaaa acggtgctgt ggacttcgcc gcactggttg aaggtggtgc
     1621 gcgcatgaac gtcgcaaacc ctgacgacag ctgggaagac cagaaacgtg acctgctggc
     1681 cttcgccagc acggcggcaa aggcggcaac agcctttgaa ctgccagcgg atgaactgtc
     1741 agaaagtctg gggaaaatcg cccagctcta caaaatccct acccgcaata ttgagcagct
     1801 cggtgatgcg ctgaactatc tggatgataa cgccatgtcg aaaggggcgg acatcattga
     1861 tgtcatgcaa cgcctgggcg gtgtggctga ccgtctggat tatcgtaaag cggcggcgtt
     1921 gggttccacc ttcctgacac tcggcgctgc gcctgaggtt gcagccagtg cagcaaacgc
     1981 gatggtgcgt gaattgtcca ttgccaccat gcaaagcaag agtttctttg aagggatgaa
     2041 tctgctgaaa ctcaatccag aagtgattga aaagcagatg acgaaggatg cgatgggaac
     2101 tatccagcgg gtgctggaga aggtgaatgc gctgccacag gataagcgcc tgtctgccat
     2161 gaccatgttg tttggtaaag agtttggcga tgacgcagcg aaactggcaa acaacctgcc
     2221 ggaacttcag cgccagttaa aactgactgc gggcaatgat gcgctcggtt ccatgcagaa
     2281 agaatccgac atcaacaagg attcactttc tgctcagtgg ttgctggtca aaaccggagc
     2341 acagaacacc ttcagcagcc tgggggaaac gctgcgccag ccgctgatgg atattctgta
     2401 cacggtgaaa agcgtcacgg gggcgttgcg tcgctgggtg gaagctaacc cggaactgac
     2461 gggcactctg atgaaagtag ccgctgtggt agctgccgtt accgtgggcc tcggcacctt
     2521 agcggtggcg ctggctgcag tgctggggcc gctggcagtc atccgtctgg gattctctgt
     2581 gctgggtatc aaaacgttac cttccgttac ggcaacagta acccgaacca gcaacgcgtt
     2641 gtcctggctg gctggcgcac cactggcact gctgcgacgc gggcttgctt catcgggcaa
     2701 cgctgcaggt ttacttactg cgccgttgtc gtctttgcgc cgcacggcat cactgacggg
     2761 aaatgtcctg aaaactgtag caggtgcgcc ggttgcactt ttgcggtctg gattatccgg
     2821 tttacgtgcg gttgctgtga tgtttatgaa tccactggcg gtactacgcg gcggactggc
     2881 tgccacaggc acggtgctgc gtatgctcgc atccggtcca ctggcgatgc tgcgcgttgc
     2941 cctgtatgcc gtctctggtc tgttaggtgc tctgctcagt ctgataggtc ttgtggttac
     3001 tgcactggcg ggtgtggcgc tggttgtctg gaaatactgg cagcccatca ccgcatttct
     3061 cggtggcgtg gtggaaggat tcaaagcggc ggcaggtcct gtcagtgccg cgttcgaacc
     3121 gcttaagcct gtgttccagt ggattggcga caaagtacag gcgctgtggg gctggtttac
     3181 tgatctgctg acgcccgtta agtcgacctc tgccgaactg cagagtgcag cggcaatggg
     3241 gcgacgattc ggggaggctc tggcggaagg gctgaatatg gtcatgcatc cgctggactc
     3301 cctgaaatcc ggcgtttcct ggttgctgga gaaacttggc attgtcagta aagaggccgc
     3361 aaaggcgaaa ctgccggaaa gcgtgacgcg tcagcaacct gcgacggtga atgcagacgg
     3421 taaagtgatg atgccatcgg gtggttttcc gtcatgggga tatggctttg cggggatgta
     3481 tgacagcggc gggtatatcc cgcgcgggca gtttggcatc gtcggtgaaa acgggccgga
     3541 aattgttaac ggcccggcaa atgtgaccag ccggagaaat acagctgcac tggctgccgt
     3601 tgttgccgga atgatgggtg ttgctgctac gccagcagag cttccaccgt tgcacccttt
     3661 ggcacttccc gcgaaaggcg gtgaagcgat ggtgagtcgt gcagccactg tgccgcccgt
     3721 tcaacggatt gaggcaccga tgcagatcat cattcaaacg cagccaggac aaagtgcgca
     3781 ggatattgcg cgggaggtgg cccgccagct tgatgaacgt gaacgcaggc tgaaggcaaa
     3841 agccaggagt aactacagcg atcagggggg atacgacgca tgatgatggt gctgggattg
     3901 tacgtgttta tgctgcgcac cgtgccgtat caggagctgc agtatcaacg cagctggcga
     3961 catgcggcaa acagccgggt aaaccgacgc ccgtcaacgc agtttctggg accggacaac
     4021 gacatgctga cgctttctgg tgttcttatg ccggaggtaa cgggcggcag gctgtcgttg
     4081 ctggctctgg agcagatggc agaacaggga aaagcatggc ccctgattga aggcagcggc
     4141 acgatttacg gcatgtatgt gattgaggga ctgaatcaga ctaaaacgga gtttttccgc
     4201 gacggtatgc cgcgccggat tgagttcacc ctgtcgctaa aacgcgtgga tgaatccctg
     4261 tccgatatgt tcggtgatct cagtgcgcaa ctgaataatc tgcagggaac ggcaacatct
     4321 gccttaagcg atatcagtaa aacggtggga gggctgctgt cgtgaatttc agctctgaac
     4381 tgcttaacaa aggcaacaaa actccggcat tcagcatcag tattgaaggc agggatatca
     4441 ccactgtgct ggacaaccgc ctgatggggc tgacgctgac ggataaccgg ggctttgaag
     4501 cggaccagct tgatctggag ctggacgacg ccgatggaaa aatcgtgctg ccgcgccgtg
     4561 gggctgtcat tacgctggcg ctgggctgga agggacagcc gcttttcccg aaaggggcat
     4621 tcacggtgga cgagattgaa cacactggcg caccggatcg cctgactatc cgggcgcgaa
     4681 gtgctgattt tcgtgaaacg ctgaataccc gccgtgaaaa gtcgtggcac aagaccaccg
     4741 ttggggaagt ggtgaaggaa atagctgcgc ggcacaaact gaagatggca ttgggtgaag
     4801 acttgtcgga taaactcgtg gagcatatag accagactaa tgagagtgac ggcagttttt
     4861 tgatgcggct ggcgcgccag tacggtgcta ttgcgtcggt gaaaaatggc aatctgttat
     4921 tcatccggca ggggcagggt aaaagcgcca gcggtaaacc actaccggtg atcactatca
     4981 cacgcaagga cggcgacagt caccgcttta ccctggcaga tcgcggagct tacacgggcg
     5041 tcattgccag ctggttgcat acccgcgaac ccgcgaagaa agaaagcacc acggtgaagc
     5101 gtaagcgcag gactaagaag cagaagaaag agccggaagc gaagcagggc gattacctgg
     5161 tgggtacgga tgaaaacgtg ctggtactta atcgcactta tgccaaccgg agcaacgccg
     5221 aacgggcagc gaaaatgcag tgggaacgcc tgcaacgcgg cgttgcatca ttctcgctac
     5281 aactggcgga agggcgggca gatctctaca cagaaatgcc tgtgaaggtc agtggcttta
     5341 aacagccgct agatgatgcg gaatggacca ttacgactct gacgcatact gtcagcccgg
     5401 ataacggttt tacgaccagt ctggagcttg aagtgaggat tgatgatttc gaaatggaat
     5461 gattcttcgc aatggagaac ttttaagttt gcaaaatgga ataatgcggt atcattattg
     5521 tgaatttagc aaaaatgggg agaactcgaa aaatgatgat ttgcccactg tgtggaagtg
     5581 ccgcccatac tcgcagcagt tttcaggtat cttcattgac caaagagcgt tacaaccagt
     5641 gccagaacat taactgcagc catacttttg ttacccatga aacttttgtt cgttcgattg
     5701 caacgccaaa agagtcaaat ccggttcagc cgcatccaat gaaatcagga caggtggcgc
     5761 tctctctttg acgctgccgc cattttgtcg ccatcgttaa aaaacagtgc ttctaacatc
     5821 atgattttaa aaggcataaa tttcagacaa caaaaaccca tcaaccttga accgaaatgg
     5881 cggggttgat gggctccaca aaatggggac atcaaagaaa agcagtggca ctaattaaga
     5941 ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa tttcattttc agggcaactt
     6001 cagttttatc ctaatcctgg ccataccatg acgatgattg tccctgccag cgtcagcagg
     6061 acgttggcga ttgcgtaggt gcccgcatag cccagcgcag ggatgttact gcgagctgta
     6121 tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc ccatcattgc gccgaacaac
     6181 agtgcgcggt tcattcgcaa tacataagca ccgaacaaga aacagataac cacgggcaca
     6241 agactgacaa ttaatccggc aatcaacatc tgaccgccaa tcgcgcccag gccgttatta
     6301 ataccgctac cggcgctcag accaacgcct gccataaaca ccatcaagcc gaactctttc
     6361 accatgctta atgcaccttg cggaatgtaa ccgaaggtcg ggtggttagc acgcataaag
     6421 cccagcataa ttccggcgaa taacaacccg gcagcgttcc ccatgccgaa actgaatgtg
     6481 ctgaactgga aggtgatcat cccgatcatc agcccaataa caaagaaggc gcaaaatgcc
     6541 agcaggtcag tgacctggct gtgaatcgag ataaagccga tgcgatcggc gatggttttt
     6601 acgcgacggg catcaccgct gacttgtaaa acgtcacctt tgttaagcac gacgttgtca
     6661 tctatcggca tctcaatctg gctacgaatg atgcggttaa ggaagcaacc gtgatcggtc
     6721 aacttcagtt gtgcgagacg tttacctaca gcgttatggt ttttaacgac cacttcttca
     6781 gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt ctttaccgtt acggaagctg
     6841 ggatcgagtc gggcatgggc gtcgggatag cctaccaacg ctatttcatc gcccatttgt
     6901 agcacggcat caccgtctgg atttgccaga atcccgttac gtcgaatacg ttcaatgtag
     6961 cagccggttt gtcgataaat acccagttca cgcagatttt tgccgtcggt ccaggccacc
     7021 agctccgggc cgacgcgata ggcgcggatc accggtaaat aaaccttacg gttggcatca
     7081 gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg tctgtaagtc ctgatgctgc
     7141 aatttcggca agtaacgcgc accaacaatc aaactcacca gaccgattaa ataggttaag
     7201 gcatacccga ggctcagatt atccagtgcc agtgagagct gcctgctttc catgccggaa
     7261 tgacgcagtg tatcgccagc accgaccaga accggtgtcg acgtcataga gcctgctaac
     7321 ataccggccg tcaggccaat atcccagcca aacagcttac ctaaccctaa ggcgatcacc
     7381 agcgcactgc caaccatcac cagtgctaac attaggtaat ttttcccatc gcgaaaaaaa
     7441 atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga aaataaacag cataaagcca
     7501 agattaagcg catcggtgtt aatgctgaaa tgttgttggc ctaataacag cgatacgact
     7561 aaaacgccaa tggaattacc cagttggatc gaaccaagtc gtaactttcc gagacatagc
     7621 ccaagcgcga ggaccacaaa taataacaga atgtaattcc catttaacaa ttcggcgacg
     7681 tttatattca cggaggctaa cttcttgttt actagtaagc tgttgaaaga aatggtaatt
     7741 tacgataatg ttttttacca gaattcaggg cgcagattca ttcagcgcac ctaaacgata
     7801 gtaaagtaac aatatatttt actagtgtaa tcacattagg tatcaacggc tatatgaatt
     7861 gcgttggcct atattagcat ggaatgcgaa gcggctttat cttactgaac gccacactgg
     7921 cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga gtgaaacata aacaacgttg
     7981 ggcgggggca atctgctgtt ttgtcctctt cattgtggtg tgcctttttc tggcgacgca
     8041 catgaaaggc gcttttcggg ctgccgggca tcctgaaatc ggcttgctgt ttttcattct
     8101 tcctggagca gttgccagtt tcttttcaca gcgtagagaa gtcctgaaac ctctatttgg
     8161 cgcaatgctg gcggcaccct gttcaatgct cattatgcgg ctgttttttt caccgacgcg
     8221 ctcattctgg caagagctgg catggttact aagcgcggtg ttctggtgtg cgctgggggc
     8281 actgtgtttc ttatttatca gtagtttgtt taaaccacag cacagaaaaa atcagtaaag
     8341 ccctcaacgc gagggcttgt cagacgatca ggcgtccaga ttttctttca cccatgcagc
     8401 aaaatcggta tagccgccga tatgttgctg atcgacaaaa atctgcggca cggtttctac
     8461 gggtttacct gccttttgtt gtagatcttc tttagtgatc ccttccgcac gaatatctac
     8521 atactgatac tgaaaatcat cgcgttcatt gctcaatttc tcagccagat cttttgcacg
     8581 cacacagtaa gggcaacccg aacgaccaaa aataacggtt tgcattattt ctctcctcat
     8641 agatttatgc ctgtaatgat cacgctaaaa tgtattcgct gaaagtaggt ttaacctgtt
     8701 gcattaattg ctaaaagcta taactgttaa acacaataca gtgaaaagtt ttagactgaa
     8761 ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta aattgcctaa aggcgtgttg
     8821 atactggaat ttatcggaat gatgctactg gcggtggcgc tgctgtcggt aagcgactcc
     8881 ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga ttctgatgat ttttctcggt
     8941 gttttgctca tgcttcccgc tgcggtggtg gttattcttc aggtggcaaa acgtcttgcc
     9001 ccacagctga tgaaccgtcc accgcaatat tcacgttcag aaagagaaaa agataatgac
     9061 gccaaccatt gaacttattt gtggccatcg ctccattcgc catttcactg atgaacccat
     9121 ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt gcgacgtcca gttccagttt
     9181 tttgcagtgt agtagcatta ttcgcattac cgacaaagca ttacgtgaag aactggtgac
     9241 gctgaccggc gggcaaaaac acgtagcgca agcggcggag ttctgggtgt tctgtgccga
     9301 ctttaaccgc catttacaga tctgtccgga tgctcagctc ggcctggcgg aacaactgtt
     9361 gctcggtgtc gttgatacgg caatgatggc gcagaatgca ttaaccgcag cggaatcgct
     9421 gggattgggc ggggtatata tcggcggcct gcgcaataat attgaagcgg tgacgaaact
     9481 gctgaaatta ccgcagcatg ttctgccgct gtttgggctg tgccttggct ggcctgcgga
     9541 taatccggat ctcaagccgc gtttaccggc ctccattttg gtgcatgaaa acagctatca
     9601 accgctggat aaagacgcac tggcgcagta tgacgagcaa ctggcggaat attacctcac
     9661 ccgtggcagc aataatcgcc gggatacctg gagcgatcat atccgccgaa caatcattaa
     9721 agaaagccgc ccatttattc tggattattt gcacaaacag ggttgggcga cgcgctaaaa
     9781 ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt ctgacagaga ggtgcagggt
     9841 gaaaattgcc atattgtccc gggatggaac gctctattcg tgtaagcggc tgcgtgaagc
     9901 cgctatacag cgcggtcacc tggttgaaat tcttgatccg ctttcttgct acatgaacat
     9961 aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag ttaccccatt ttgacgcagt
    10021 gatcccgcgt attggcaccg ccattacctt ttatgggacg gcggcactgc gccagttcga
    10081 gatgctgggg agctatccgc tcaatgagtc ggtcgccatt gcccgggcgc gtgacaaatt
    10141 gcgttccatg caactgctgg cgcgtcaggg catcgacctg cctgtcacgg gcattgcgca
    10201 ttcgccggat gataccagcg atttaatcga catggtcggt ggtgcgccgc tggtggtcaa
    10261 gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg gagacgcgtc aggcggcgga
    10321 aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt ctggtgcagg aatatatcaa
    10381 agaggcgcaa gggtgcgata tccgctgtct ggttgttggc gatgaagtgg tcgctgcgat
    10441 tgaacggcgg gcgaaagagg gcgattttcg ttccaatttg catcgtggcg gcgcggcaag
    10501 cgtcgccagt atcacaccac aggagcgtga aatcgcgata aaagccgcgc gaacgatggc
    10561 gctggacgtt gctggtgtgg atattctgcg tgctaatcgc gggccgttgg tgatggaagt
    10621 gaatgcgtcg ccggggctgg aaggaataga aaaaaccact ggtatcgaca tcgcgggtaa
    10681 aatgatccgc tggatcgaac gctacgctac gacagaatat tgcctgaaaa cgggtggtta
    10741 gtcgcaatca cattactgat catggttttg cctgcgcttt ttgcgtaagc tgtgccggtc
    10801 tttttatcga aagaggttgt acaaaattat gacatcgctg gtcgttcctg gtctggatac
    10861 gctgcgtcaa tggctcgatg acctggggat gagttttttt gaatgtgata actgtcaggc
    10921 tctgcatctg ccccatatgc agaatttcga cggtgtcttt gatgccaaaa tcgatctgat
    10981 cgataacacg atcctgtttt ctgccatggc ggaagtccga ccttcagccg tattgccgct
    11041 ggcggcggat ttatctgcca tcaatgccag ttcgctgacc gtgaaagcat ttcttgatat
    11101 gcaggatgat aatctgccaa agctggtggt ttgccagtct ttatccgtta tgcagggcgt
    11161 aacctacgag cagtttgcat ggttcgtgcg tcagagcgaa gagcagattt cgatggtcat
    11221 tcttgaagct aatgcccatc aactgctgtt accgactgac gatgaagggc aaaacaacgt
    11281 taccgaaaac tatttcctcc actgataact cctttcgagc acgcagtcgc tggtgcagtg
    11341 actgcgcact gcaaaattat ctgctgtttt taaccttttc ttaaagatta tttcacttct
    11401 cttgtgccga tttggcttta tcacatagag caaatacgca taacaatttg ttaaataccg
    11461 ttttttaatc cgagctatag tctcaaaccc tggctaaagt tattcttgcg atgcttttat
    11521 atagcgagca gtgctggccg ggagagagtt atcttttctt acaccgcgcc gataaaaaat
    11581 atgcacgttt attgcatatc ttttagtatg acaacttttg ttcgtttgtt aacgaacttt
    11641 cagaaggaaa gagatatgac cgccttaaat aaaaaatggc tttcgggtct ggttgcgggt
    11701 gctctgatgg ccgtctctgt cggcacgctc gcggctgaac aaaaaacact ccacatttat
    11761 aactggtctg attatatcgc ccctgacacg gtggccaatt ttgaaaaaga aaccggtatt
    11821 aaagtcgtct acgatgtttt cgactctaac gaagtactgg aaggcaaatt aatggcgggt
    11881 agcaccggct ttgacttggt ggttccatct gccagctttc ttgaacgcca gttgactgcg
    11941 ggagttttcc agccgctgga caaaagcaaa ttgccggagt ggaagaatct cgacccggag
    12001 ctgctgaagc tggtcgccaa acacgatccc gacaataaat ttgctatgcc ctatatgtgg
    12061 gcgacgaccg ggattggcta taacgttgat aaagttaaag ctgtgctggg cgaaaacgcg
    12121 cccgtcgata gctgggactt gatcctcaaa cctgaaaatc tggaaaaact gaaaagctgc
    12181 ggtgtctctt tcctggatgc gccagaagaa gtttttgcta ccgtgttgaa ttatctcggc
    12241 aaagatccca acagcactaa agcggatgat tacaccggac cggcaaccga tctgctgtta
    12301 aagctgcgcc cgaacattcg ttatttccat tcatctcaat acattaacga cctggcaaac
    12361 ggcgatattt gcgtcgctat cggctgggca ggtgatgtct ggcaggcgtc aaaccgcgcg
    12421 aaggaagcga agaatggagt gaatgtctcg ttctcgattc caaaagaagg ggcgatggcg
    12481 ttctttgatg tattcgccat gcctgcggat gccaaaaaca aagacgaagc ctatcagttc
    12541 ctgaattacc tgctgcgccc ggatgtagta gcgcatattt ccgaccatgt gttctatgcc
    12601 aacgccaata aagcagccac gccgctggtg agtgcggaag tccgtgagaa cccgggtatt
    12661 tatcctccgg cggacgttcg tgcgaagcta ttcactctga aagtacagga tccgaaaatc
    12721 gaccgtgtgc gcacccgcgc gtggaccaaa gtgaagagcg gaaaataatc cgcagcctta
    12781 gatgccgggc gggcgcacca cacccgccgg caattcgcac catcatggtg cgcttgcaca
    12841 cattcaatgc cggagagcag ccgtgaatga cgctatccct cgcccgcagg cgaaaacccg
    12901 taaggcgctg acgccgctat tagaaatccg caacctcacc aaatcctacg atggtcagca
    12961 tgcggtcgat gatgtcagcc tgaccatcta caaaggtgaa atcttcgcgc tgctgggcgc
    13021 ttccggctgt ggcaagtcca cgctgctgcg tatgctggca ggttttgaac aaccttctgc
    13081 cgggcagata atgctcgatg gcgtcgattt gtcacaggtg ccgccttacc tgcgccccat
    13141 caatatgatg tttcagtctt acgcgctgtt tccgcatatg accgtggaac agaacatcgc
    13201 ttttggcctg aaacaggaca aactaccgaa agcggaaatt gccagtcggg tccatgagat
    13261 gctcggcctg gtgcacatgc aggagttcgc caaacgcaaa ccgcatcagc tttccggtgg
    13321 tcagcgacag cgtgtggcgc tggcccgaag ccttgccaag cgcccgaaac tattactgct
    13381 cgacgagccg atgggcgcgc tggataaaaa gctgcgtgac cggatgcagc ttgaagtggt
    13441 ggatattctg gagcgtgtcg gcgtgacctg tgtgatggtc actcacgatc aggaagaggc
    13501 gatgacgatg gcgggacgta tcgccattat gaatcgcggg aaatttgtcc agattggcga
    13561 gccagaagag atctacgaac atccaaccac ccgctacagt gctgaattta tcggttcggt
    13621 taatgtcttt gaaggtgtac tcaaagagcg tcatgaggat gggcttgtgc tttattcgcc
    13681 tgggctggtg catccactga aagtcgacgc ggatgcctcg gtggtcgata acgtgccggt
    13741 acatgtggcg ctgcgcccgg aaaaaatcat gctttgcgaa gagccgcccg ccaatggttg
    13801 taacttcgcg gtgggggagg tgatacacat tgcctatctc ggcgatcttt cggtgtatca
    13861 cgttcgtctg aaaagtgggc agatgattag cgcccagcta caaaacgccc atcgtcatcg
    13921 taaagggtta ccgacctggg gcgacgaagt gcgtttgtgc tgggaagtgg acagctgtgt
    13981 ggtgctgacg gtttaaggag caaagatgaa tacacttgaa cctgctgccc agtcgaaacc
    14041 gccgggcgga tttaagctgt ggttgtcgca gctgcaaatg aagcatgggc gcaaactggt
    14101 cattgcgttg ccatatatct ggttgatctt gctgtttctg ctgccatttc tgattgtctt
    14161 taaaataagc ctggcggaga tggcgcgcgc tattccacct tataccgaac tgatggagtg
    14221 ggctgacggg caactgtcca tcactcttaa tctcggtaat tttctgcaac tgaccgacga
    14281 tccgctctat ttcgatgctt atctccagtc gctacaggtg gctgcgattt cgacattttg
    14341 ctgtttactg atcggctatc cgctggcgtg ggcggtggcg cacagtaagc cttcgacccg
    14401 taatatttta ttactactgg tgatcctgcc gtcgtggacc tcgtttctga tccgcgttta
    14461 tgcctggatg ggaatattaa aaaacaacgg tgtgctgaat aattttctgc tgtggctggg
    14521 ggttatcgat caaccgctga ccattcttca taccaatctg gcagtttata ttggcattgt
    14581 ttacgcctac gtgccgttta tggtgctgcc gatttatacc gcgttgattc gtattgatta
    14641 ttcgctagtg gaagcagcgc tggatctcgg tgcacgaccg ctgaaaacgt tctttactgt
    14701 gatcgtgccg ctgactaaag gtgggattat tgccggatcg atgctggtgt ttatcccggc
    14761 tgtgggcgag tttgtgatcc cggaactgct cggtggcccg gacagcatca tgatcgggcg
    14821 cgtgctatgg caagagtttt tcaataaccg cgactggccg gtggccgcgg cggtagcgat
    14881 catcatgttg ctgctgctaa ttgtgccgat aatgtggttc cacaaacacc agcaaaaaag
    14941 cgtgggagaa cacggatgaa taatttaccg gtagttcgtt cgccctggcg gattgtgatt
    15001 ttgctgctgg gctttacctt tctctacgcg ccaatgctga tgctggttat ctattcgttt
    15061 aacagctcga agctggtgac ggtgtgggcc ggctggtcaa cgcgctggta tggtgagtta
    15121 ttgcgcgatg acgcgatgat gagtgcggtt ggtttaagcc tgacaattgc tgcctgtgcg
    15181 gcaacggcgg cggcgatcct cgggactatt gcggcggtgg tgctggtgcg ctttggcagg
    15241 tttcgcggat caaatggctt tgcctttatg atcacagcgc cgctggtaat gccagatgtc
    15301 atcacgggct tgtcgctgtt gttattattc gtcgcgcttg ctcatgccat tggctggcct
    15361 gcggaccgcg gtatgcttac catctggctg gcgcatgtca ctttctgtac ggcttatgtg
    15421 gcggtcgtta tttcgtcgcg tctgcgggaa ctggatcgct cgatagaaga agcagcgatg
    15481 gatctcggtg cgacgccgct gaaagtgttt ttcgtcatta cgctaccgat gatcatgcct
    15541 gcgatcattt ctggctggtt actggctttt actttgtcgc ttgatgatct ggtgatcgcc
    15601 agctttgttt ccgggccggg agccaccacg ttaccgatgc tggtcttttc cagcgtgcgg
    15661 atgggggtga atccggaaat caacgccctt gcaacgttaa ttctcggtgc ggtcggaatt
    15721 gtcggattta tcgcctggta tctgatggct cgcgcagaaa aacagcggat acgcgatatc
    15781 cagcgtgcaa gacatggctg aagacactaa aatttgccaa cctggctaca taatgccgcg
    15841 catgccgcgg cattgttttc atggaagacg aaacgttggg attttttaag aaaacatctt
    15901 catctcatgc tcgcctgaat gtgcctgcgc tggtgcaggt agcggcgctc gccattatta
    15961 tgatccgtgg cctcgacgtg ctgatgattt tcaatacgct gggcgtgcgc ggtattggcg
    16021 agttcattca tcgcagcgta caaacctgga gcttaacgct ggtattttta agcagtctgg
    16081 tgctggtttt tatcgagatc tggtgtgcgt tttcactggt gaaagggcgt cgctgggcgc
    16141 gctggctata tctgctgaca caaatcaccg ccgcaagtta cttgtgggcg gcttcgctgg
    16201 ggtatggtta tccggagctg ttcagcattc ccggtgaatc aaaacgtgaa atcttccata
    16261 gcctgatgct gcagaagctg ccggatatgc tcatcctgat gctgctgttc gttccctcga
    16321 ccagtcggcg gttcttccag ttgcaataat gtgtataatc gtcgcccctg atgatgtgaa
    16381 ggtcaatgta tgcagtgcgc actttacgac gcgggtcgct gtcgttcctg tcagtggata
    16441 acgcagccga ttccagagca actctccgct aaaaccgccg atcttaaaaa tctgctcgcc
    16501 gattttccgg ttgaggaatg gtgcgcgccg gtgtcaggcc cggaacaagg gtttcgtaat
    16561 aaagccaaaa tggtggtgag tggtagcgtt gaaaaaccac tgctcggtat gctgcatcgc
    16621 gatggcacgc cggaagacct ttgtgactgc ccgctttatc ctgcctcttt tgcgcccgtt
    16681 tttgcggcgc taaaaccgtt tatcgcccgt gcggggttaa cgccctacaa cgtggcgcgt
    16741 aagcgtggcg aactgaaata cattctgctg actgaaagcc agagcgatgg cggcatgatg
    16801 ctgcgctttg tactgcgttc tgaaaccaaa ctggcacaac tgcgtaaggc gctgccgtgg
    16861 ttacaggaac aactaccgca actgaaagtt attaccgtca atattcagcc ggtacatatg
    16921 gcgattatgg aaggggagac ggagatctac ctgactgaac aacaggcgct ggcagagcgt
    16981 tttaatgacg taccgctgtg gatccgtccg caaagtttct tccagactaa tccggcggtc
    17041 gccagccagc tgtacgctac cgcgcgcgac tgggtacggc agctgccagt taaacatatg
    17101 tgggatctgt tctgcggtgt ggggggcttt ggtttacact gcgcgacgcc tgacatgcag
    17161 ttaaccggga tcgaaattgc gccagaggcc attgcctgtg caaagcagtc agccgctgaa
    17221 ctgggcttaa ctcgtttaca atttcaggcg ctggactcca ctcagtttgc cactgctcag
    17281 ggggaggtgc cggagctggt gctggttaac ccgccgcgcc gcggcattgg taaaccgctg
    17341 tgtgattatc tctcaacgat ggcaccgcgt tttatcatct actccagctg taacgcccaa
    17401 actatggcga aagatatccg cgaactgcct gggtttcgca tcgaacgggt acagcttttt
    17461 gatatgttcc cgcacaccgc gcactatgaa gtgctgacgc tgctggtgaa gcaatctgaa
    17521 gccgcaggtg cggcttcaga ttgctgatca ggtgcgcttt gttcatgccg gatgcggcgt
    17581 gtacgcctta tccggcctac aaaatcatgc taaatcaata cattgcagag ttgatgtggg
    17641 ccagataagc gcagcgcatc aggcaatctt acttttgtca tcagtctcaa ttactgcggg
    17701 aaccactggt cattgatttt ctgatatgta ccgtcagctt taattgctgc cagcgcgtta
    17761 ttcagttttt ccagcagagc tttgttatcc ggacgtacag cgatgcccag accggtgccg
    17821 aagtattgcg gatcggtcac tttttcagta gcaacgccca gttgcggatt ggttttcagc
    17881 cattcgttta ccaccgctgt gtcaccaaat accccatcaa tacgaccatt tttcagatcg
    17941 ataaaggcat tctgataact gtcataggag acagttttca cttcggggtg ctgatcctga
    18001 atatatttct ggtgcgtggt gccgttttcc atcccaatac gtttgccttt cagatcggca
    18061 aacgttttgt aggtatcttt tttggcaatc acgacggctg agttttcata gtagggcgtg
    18121 gtaaacgata cctgcttgct acgctcgggc gtgatatcca taccggagat tacggcgtca
    18181 tattttctga atttcagtga cgggatcagg ctgtcgaacg cgtgattagt aaaagtacat
    18241 tctgcctgca tttgtttgca caaggctttt gccagatcga tatcaaagcc gacaatctca
    18301 ttattagcac ctatagattc aaagggtgga taggtggctg aaacgccaaa attgattttc
    18361 tctgcggcag aagcaccgaa agtaaaggaa gcaagtaaag cggcaagaac taactttttc
    18421 atgatggaac tcccgtctgt caatcttatg atttttggcc gtgtctgcgg catgggataa
    18481 caatgccatc aagtgaattt atatgcaata aatatgatta aataatttaa atgaaataaa
    18541 aaacgacgga taacttagtg agttgtccgt cttcattata agaatttatg cactatgtag
    18601 gccggataag gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg
    18661 ctggcgcgtc ttatcagacc tacaaacccc ccggcgaatg tacgcagcta cattaattcc
    18721 gccgttcgaa tgccagtgct ttgcgctcga tcagacgcat catcagcgtc agcaggccgt
    18781 tgacgaccag gtaaataatc cctgccgcac cgaacaccat tacatcgtag gtgcgtccgt
    18841 acaacaactg gctgtatccc atcacttcca tcagcgtaat ggtgtaagcc agagaggtac
    18901 ttttgaacac cagcaccact tcgttggaat aagaagagag cgagcgttta aaggcatacg
    18961 gcagcaggat cgccagcgta tcttttttgc tcattcccag ggcgctacag gactgccact
    19021 gaccttctgg gatcgcacga attgcaccgt aaaacagctg cgtggtatac gccgcactgt
    19081 tcagcgataa cgcaatcagc gcacataacc acggttctga caacaaatgc cacagtgccg
    19141 gatactcctg caaagtcgga aactggcccg gcccgtaata aatcaggaag atctgcacca
    19201 gcagcggcgt accggtaaac agcgtgatat aaccccgcac cagccacacc agcaccggcg
    19261 ttttcagcgt caggatgatg gtaaaaatca atgccagaat cagtgccaca atcagcgagg
    19321 caacggttag cgtcaggctg gtgtgtagcc ctttcatcag ttcgggtaaa tactcaaaca
    19381 ttagctgggc ctccgctcaa aacgtgtcgc gcgcaggtca atgcgtttga gaatgtactg
    19441 actgagcagg gtgatcacca ggtaaatcgc cgccgccaca atataccagg taaatggttc
    19501 ctgggtacga gtagcgatgc tttttgtttg cagcattaaa tcattcacac taatcaaact
    19561 gaccagcgcg gtatctttca gcagcaccag ccactggtta ccgaggccag gcagcgcatg
    19621 acgccacatc tgcggcatca ccagacggaa aaagatagcc gatttcgaca gccccagcgc
    19681 ctgaccagac tcccactgac ccaccggcac cgctttcaac gcgccccgaa gcgtttgcga
    19741 ggcataggcg gcatacagca gtgacagagc gatgacacca caaaggaacg ggctaacgtc
    19801 gaagttctca atgtccatct gcactgggat ctgcacgaac ccaagattga tagtgaagcc
    19861 atccgaaagc gtcagcagca gctgcgagga gccaaaatag ataaacagca ccaccagaat
    19921 ttctggcagg ccacgcagaa tggttaccag cgctgaacct gcccacgcga caggacgcca
    19981 tttttccgac tcccataccg caaagaacat cgccagcgcc agcccgacaa tcaatgcaca
    20041 aacggcaagg ccgacggtca tcccggcggc gcttgctaaa ggaaaaaatt cattcatcag
    20101 gaattacttc tggaaccatt tgttgtagat ggtttcgtaa gtgccatctt tcttcacttt
    20161 ttccagcgca gtgttgagtt tctgctgcag ctcagtgttg ccctgacgta ccgcgatgcc
    20221 gaggccagtg ccgaagtaat ctttatcggt cactttgtcg cccaccgccg ccagtttcgg
    20281 gttatctttc agccactcag tgaccactgc ggtgtcaccg aagacgccgt cgatacgccc
    20341 gttttgcaga tccagttttg cgttctggta gctgttatac ggaacggtag tgatttccgg
    20401 gtgcttatcc ataatgaatt tctggtgtgt cgtcccgttc tgtacgccga cttttttgcc
    20461 tttcagctga tcaacactgg tgtatttgcc ttgctgaccc acaaacaggg cagagttgtc
    20521 atagtacggg gtggtaaaca gcacctgctt ttcacgctcc ggagtgatat ccatgcccgc
    20581 catcacggct tctacgcgac ggaatttcag gcttgggatc aggctgtcaa acgcctggtt
    20641 agagaaagtg caggttgcat caatctcttt acacagcgct tgtgccaggt cgacgtcaaa
    20701 accaacgatc tggttgtttg catcaatcga ttcaaacgga ggataggatg cttcggtagc
    20761 aaaacgaatg gtttcggcag ctgtggcgga aagactaaaa cctgcaatta acgcggcaat
    20821 cagaactttt ttcattgttg ttatcccgaa tcttagtgag agagatagtt tttaaatgct
    20881 tcggtttgcg gctcggtaaa gcagctcgcg tcgccttgtt ctacgatatg accattttcc
    20941 atatacacca ctcggctggc ggttttacgc gccacttcaa cttcgtgggt gacgatcacc
    21001 tgggtaatat tcgtttctgc cagctcacga atgatgctga cgatttgtgc cgtaatttcc
    21061 gggtccagtg cggcggtcgg ttcatcgaac agcagtacct gcggttccat catcaacgca
    21121 cgggcaatag caacacgctg ctgctgacca ccagaaagat gcagcgggta acgatcgcta
    21181 taaggtttga gacgcagacg ttccagcagt ttttctgcac gggccagcgc ctgatcttta
    21241 ctcaacccca gtacacggca gggcgcttca atcaggtttt gctgcacggt cagatgcggc
    21301 cacaggttgt attgctgaaa caccatgcca acgttacgac gcaaatcgcg aatcgctttg
    21361 tcagagggtg ttttggtgaa atcgaaatgg ttgcctgcaa tgttgagcgt accggagcgc
    21421 ggcatctcaa gcagattgag tacacgcagc agcgagcttt tacccgcgcc gctggggcca
    21481 agtaacacca gcgtttcgcc ctgtgggcaa tccagcgtga tatcgaacag cgcctgatgc
    21541 gcgccgtaga agcaattaat gccgtttaat tgaatactca ttgacactcg tatactggca
    21601 gtctgatagc tattgaggtc gaagatagta cctttgacag aataattatg caatatttct
    21661 gctttaaaag ttaaaagcaa agcgcattat tcaataaaca tagcacaaaa taacgggggc
    21721 ggtggtcggc gagcataaat gtcggcattc ctcacgaaat gccggacaat ttacggggtt
    21781 tattggttga tcaaggcgtt agcgattctc gatggactga cggagcgtac ccgccgtggc
    21841 atgaacgctg ccgcctaagt agcgtacatc gtcgatcacc caacactggc cttcctgaat
    21901 cattaacact tcatcctgcc agctctgatc accctgtttt agatccacac gcagaggaat
    21961 atttcgcgca tcacgattcg ggattgttga ggcactggca acacgggcgc tctctggcaa
    22021 ggttgtgcga ctggagaatg gatcgttggt cagtagttcc cgatggttat tatcccggga
    22081 ggcatcgcta agcagtgtcg ccagtttgtc gctcagataa gggcgcaagg cggtgatgtc
    22141 gttgctgcgg tgcaaaatgc ggtagtcata aaattgctgg gccacgttac ccgggcctcc
    22201 ttcaacgcaa ggaccactgc gtgtgccgtt atctttataa gctggagtga ctgtggtgca
    22261 ggcactgagg agcagtgcgc aggggataag cattgtcaat ttgctgtagc gcataatgat
    22321 ttccttataa gcgatcgctc tgaaagcgtt ctacgataat aatgatatcc tttcaataat
    22381 agcgtatcag tctgataatg cttttgagat cgaaggctta gcaaacaagg agatcgatca
    22441 tgcaattttc tacaacccca actctggaag gccagaccat cgttgaatat tgcggtgtgg
    22501 tgaccggcga agcgatttta ggtgccaata ttttccgtga tttctttgcc ggtatccgcg
    22561 atatcgttgg cggacgttcc ggtgcgtatg aaaaagaact gcgtaaagca cgggagatcg
    22621 cctttgagga attaggctcc caggcgcggg cgctgggggc cgatgccgtc gtcggtattg
    22681 atatcgacta cgaaacggtc gggcaaaacg gcagtatgct gatggttagc gtcagcggta
    22741 cggcggtgaa aacgcgtcga tgagaagagt cttctggctg gtcgctgccg ctctgttatt
    22801 ggcagggtgt gcaggcgaaa aaggcattgt cgagaaagag ggatatcagc ttgatacccg
    22861 acgccaggcg caggcggcgt atccgcgcat taaagtgctg gtgatccact acaccgcaga
    22921 tgattttgat agctcgctgg cgacactgac cgataagcag gtcagctcgc attatctggt
    22981 ccctgcggta ccaccgcgat acaacggtaa accgcgcatc tggcaactgg tgccggaaca
    23041 agaactggcc tggcatgcgg ggattagcgc ctggcgcggg gcaacgcgcc ttaacgacac
    23101 ctctattggc attgagctgg aaaaccgtgg ctggcaaaaa tcggccggag tgaaatattt
    23161 tgccccgttt gaaccggcac agattcaggc gcttattcca ctggcgaaag atattattgc
    23221 ccgttatcac atcaagccgg aaaacgtagt ggcacatgcg gatatcgcac cgcagcgcaa
    23281 agacgatccg gggccattat ttccctggca gcaactggcg cagcagggga ttggtgcctg
    23341 gccggatgcg cagcgggtta acttttacct tgccgggcgc gcgccgcaca caccggtaga
    23401 taccgcgtca ttgctggagc ttttggcgcg ctacggttat gacgttaaac ctgatatgac
    23461 accgcgcgag cagcggcgcg tgattatggc attccagatg catttccgcc cgacgttata
    23521 taacggcgaa gcggatgcag aaactcaggc gattgccgaa gcattgctgg agaaatacgg
    23581 gcaggattag cgcggcagtt ttccgtggtc gcgtagccag gcggcagttt tctcgatacc
    23641 ttcatccagg gtgatgaccg gctgataacc taactcttcc tgcgcacgcg taatatccag
    23701 cgtaaagtca aaattcaact tggagacgcc gtagtgggtc agcggcggct cttttgctga
    23761 cttgcggaaa cgctccatgc tgcgggcgat catatccagc atcgggtagg ggacggaacg
    23821 aatacgacag tcaatattca actcgtcgat cagcttctgc acgatgctgc acagtgtgcg
    23881 atgctcgccg ttggtgatgt tgtacacacg cccggaaggt agcttatcgc aggcttcctg
    23941 gcttgccagc cacattgcgt gcacggcatt ttcatagtag gtcatatcga ccagcgcact
    24001 gccgccatgc ggtaacagaa tactgccgta gtggtgcatc atatgcgcca gacggggaat
    24061 aaagacttta tcgtgcggtc cgaacagact ttgtgggcgc agaatagtaa agcgcgtttg
    24121 tggattcgcc tgcgaaaaca tattgatcac ttcttcgctg gctgctttgc tgcgggcaaa
    24181 ctcgttggcg aagcggtgag ggcgaaaatc ttctttaata tcgcgatggt ggtgataatc
    24241 gaagtacagg gagggggaag agatatgaat aaagttacgc acaccccagg cgacagccca
    24301 ttcacccaag cggcgagtgg cgcgaacgtt agccagatcg aaagcctgtt gtgtccccca
    24361 gggtgaggta aagctggagc agtgccacag cgtatcaatg cccgcgagca tcactttagc
    24421 ttgtgatgaa accagctcgg tcagatccgc cggaacaaac tctgcgccca ttttttccag
    24481 caatttgccc attgcctcgt tgcgaccggt cgctcgcacg ctgatgcctt tctggcataa
    24541 aaactctacc gcgtttcgac ctaagccgct ggtggcgccg gtaaccagta ccttcatatc
    24601 aatccactgt tgttgagaaa ataacgtgcg cattcttccg tgatttcccc catgatgcaa
    24661 tgggaaacat gaaagaataa cgcaggtttt gtcgattaat ctgtgctttg ttctgccagt
    24721 ctggcgattt gttttgccat tccgcgaaaa ataaacagat gcgcggggat catcaataac
    24781 cagtaaaaca gccccggcat accgtgcgga tgccagaaag cgcggacatc gatagtacga
    24841 tagtcgcctt tatcttccag gctaaaacac agtcgtccca gccccggcgc tttcatgcca
    24901 aataacaacg taagttgttt ttccggttca acgacaatca ctttccagct atctaccgca
    24961 tcgccagtct gtaaatattc gcgctccggg cggcctttcg ccagcttatg accgatcgcg
    25021 cggtccatca acgcccgtgt ctgccacaaa atattgccaa agaaataacg ctctttaccg
    25081 ccgatttggt tcactacctg ccataaagca gcaaggctgg cggacgtttt aacggtaaac
    25141 cccgcctgtt tggcaaaata accgtactcc ggtcgccagc gggcaaaggc ctgagcgtcg
    25201 tagccccagt cgctggagtt gaccagtttt tcctcctctt tcaacgtgct acgtaccgcg
    25261 tcatcgaaag cgatcagccg ttgtgggatg agtgcacgta gcgcggtatc atccgccagc
    25321 agatcgtgtt tcagcccctg aatcaacgcc ctggcggtgg tgggcggtac ggaagtaatc
    25381 acattgagaa accacaccga aatccagcgg gtggggaggg ggatggggat caaccagcgg
    25441 cgcttgccgc tcaccgccat aaaatgttca aactgttgct gataactgag cacctctggt
    25501 ccggcggctt cgaagatgcg gtgttcgctg gccggatgat ctaacaacgc caccagatag
    25561 tgcagcaagt tttccagcgc gatgggcgtg gtgcgtgaac gtacccagcg tggcggcgtt
    25621 aacactggca ggttgtagac catatcgcgc atgacttcga acgccgctga acctgcgcca
    25681 acgataattc cggctcgaag ttcggtcaca ggtacattcg cttcacgaag aatgtccgcc
    25741 gtagcctgac gagcacgcag atgatccgac tgctcatgtg gcggggcctg caacgaactg
    25801 agaaagatta attgcttaac tggtacttca cgtagcgcat cgcggacgtt gagagccacc
    25861 tggcgctcct gagcgataaa atcgccgcct tcgcccatgc tgtgcaccag aaaatagacg
    25921 gtatcgatat cctgcaacag ggccggaagg ttatccggcc agctgagatc gactttatgg
    25981 caactgacgt ttgccagttg cagctttgca agcctgtcga catgacgtgc cgccgccagg
    26041 atctgatgcc cttgctggct gagtgtgcgc accagatgct gaccaatgta gccactggca
    26101 ccgagaacta aaatgcgttg cggcacgtct ctctccttaa cgcgccagga atgcgcgcca
    26161 gtgggcggcg acttccgcca gttgttcgcg cgagacgtca agatgcgtca ccaggcggac
    26221 aatcggcgag gcgttaatca gcacgtttct cactttcatg tattcgccta acgcggcagc
    26281 attttcttcc ccgacgcgaa caaacagcat attagtgtcc tgacgcatca catccgcgcc
    26341 tgcttcacgc agttgctccg ccatccaggc ggcgttgtcg tgatcttcct gcaaccgtgc
    26401 gacgttattt ttcagggcat acatcccggc ggcagccaga atcccggact gacgcatccc
    26461 gccaccggcc attttccgcc agcggatggc acgtttaatg taatcacgat taccgacgag
    26521 taatgaaccg acaggcgtcc caagaccttt cgacaggcaa atggtgaacg aatcacaata
    26581 ttgcgtgatc tctttcagtt cgcagccgta agccaccacg gcattaaaga tgcgcgcacc
    26641 gtcaacatgc agcgccagat tgcgctcgcg ggtaaattcc catgcttctt tcaggtattc
    26701 acgcggcagc actttgccgt tgtgggtgtt ttccagactg agtaatttgg tgcgggcgaa
    26761 atggatatcg tcgggtttga ttttcatcgc cactttatcc agcggtagcg tgccgtcggc
    26821 agccgcgtcg atgggttgcg gctgaatgct gcccagcacc gctgcgccac cggcttcaaa
    26881 cagatagtta tgcgcggcct gaccgacaat atactcttcg ccacgttcgc agtgactgag
    26941 cagagcgacc aggttggcct gagtgccggt aggcagaaaa atggcggctt ctttaccgga
    27001 aagctctgct gcatagtcct gcagagcatt aacggtaggg tcgtctccgt aaacgtcgtc
    27061 cccaaccggg gcggccatca tcgcttcgag catggcgcgg ctcggtcggg taacggtatc
    27121 actgcgtaaa tcaatcatgg catgtcctta ttatgacggg aaatgccacc ctttttacct
    27181 tagccagttc gttttcgcca gttcgatcac ttcatcgccg cgtccgctga tgattgcgcg
    27241 caacatatac aagctaaatc ctttggcctg ttcgagtttg atctgcggtg gaatggctaa
    27301 ctcttctttg gcgaccacca catccaccag caccggaccg tcgatggaga aggcgcgttg
    27361 cagggcttca tcaacttcag acgctttttc tacacggata cccgtaatgc cgcacgcttc
    27421 ggcaatgcgg gcaaagtttg tgtcgtgtag ttcggtgccg tcagtcaaat agccaccagc
    27481 tttcatctcc atcgccacaa agcccagcac gctgttgtta aagacgacaa ttttcactgg
    27541 cagtttcatc tgcactactg agaggaaatc gcccatcaac atgctaaaac cgccatcgcc
    27601 gcacatggcg accacctgac gttctggctc tgtcgcctgc gcacccagcg cctgcggcat
    27661 ggcgttagcc atcgaaccgt ggttaaacga acctaacagg cgacgcttgc cgttcatttt
    27721 tagataacgt gccgcccaca ccgttggcgt accaacgtca caggtgaaaa tagcgtcatc
    27781 ggcggcaaaa tgactaattt gctgcgccag atattgcggg tgaatggctt tctcgctcgg
    27841 tttagctaaa tcgtccagcc ctttgcgggc gtcgcggtaa tcttccagcg ctttatccag
    27901 aaacttgcga tcggcttttt cttccaccaa tggaagcaat gcacgcagag tcgacttgat
    27961 atcgccgacc agtgccatat ccaccttgct gtgagcgccg atgctggctg ggttgatatc
    28021 aatctgaatg attttggcat cggtcgggta gaaggcgcgg tagggaaatt gcgtgccgag
    28081 tagcactaac gtgtcggcgt tcatcatggt atggaaacct gacgagaagc cgattaaccc
    28141 ggtcattcca acatcatacg gattatcgta ttcgacatgt tctttaccgc gcagggcatg
    28201 aacaataggc gctttaattt tcccggcaaa ctcaactaac tctttatgcg cccccgcgca
    28261 gccgctgcca cacatcaggg cgatattgct ggaataacgc agcagttgcg ccagtttgcg
    28321 taactcttct tcttccggcg tcacgactgg ttgtggcgca tgataccagt gcgtggttgc
    28381 cccttctggc gcaggtttta acgccacgtc gcctggtaac acgacaaccg aaacgccacg
    28441 gttaagcacc gctttgcgca tggcaatcgc cagtacttgt gggatctgct ccgggctgga
    28501 aaccagctcg caatagtgac tacattcgcg gaatagctct tgtgggtggg tttcctggaa
    28561 atagccgctg ccaatttcgc tggagggaat atgagcggca atcgccagta ccggaacgtg
    28621 attgcggtgg caatcaaaca ggccgttgat taagtgcagg ttgccggggc cgcacgatcc
    28681 ggcgcagacc gccagttctc cgctaagttg tgcttcagcg ccagcggcaa aggccgccac
    28741 ttcttcgtgg cgggtggaca tccactcgat ggtgcccatg cgattaagac tgtcactaag
    28801 accattcaga gagtcgcctg tgactcccca gatgcgtttc acccctgccg attcgagtgt
    28861 tttggcgata taagctgcaa ccgtttgttt catggttctc catctcctga atgtgataac
    28921 ggtaacaagt ttagttcatc tgacggaggg ggaagggatg ggagagaaag gaggcactaa
    28981 cggttaaata gcccgatgaa aggaatatca tcgggcataa ggcgattatg cgagaaccaa
    29041 atccccctgc ggatggcagg agcaggccag tacgtaacct tcagcgattt cggcgtcggt
    29101 cagcgtcatt gtactgctca ccgtatattc accggaaacc acttttgtct tacagcagcc
    29161 gcaaacaccc gcacggcagg cagcgacaac cggaacgtta ttgctttcca gcgcctccag
    29221 tagcgtggtg ccaaccgggg cgtaaaattc tcgtgccggt tgcagtttgg tgaatttcag
    29281 accgctggtc gccgcttttg ctactggggt gaagaatttc tctttaaaga aacgcgtcac
    29341 gccgagcgct ttcacttcct gctctaccca atccatatac ggagccgggc cgcaggtcat
    29401 cacggtacgt gaagctaagt caggtacacc tgccagcagt tctcgagtga gacgaccagc
    29461 gataaagcct tcggtaacgt tattttctgc caccagcgtt accggatagt tacgccactc
    29521 atcggcgaaa ataacatcct gcggcgtatg cacgttgtag atcacccgca catcggcctg
    29581 tggacggttc ttcgcaagcc agcgacgcat cgacataatc ggcgtgacgc cgcagcctgc
    29641 cgccagcaac aggaatttat cttctgcttt atcgtcgcag gtaaattccc ccatcgcgtc
    29701 cgaaagccag agataatcac cgcgttttac atcgcgcgtc agccactggg agccgacacc
    29761 gtcatcaatc cgccgcacgg tcagggtgat atattcactc acgcctggcg tggaggaaat
    29821 ggtgtaagca cgcagcgttt ccgctgagtt tcgcacgctg accagtgcat attgcccggc
    29881 gcgatatggg tagtaatcgt ggcaaatcag ggaaatcgtc cacacatccg gcgtttcttg
    29941 cgtaatgtga tgaacctgca tccgccacgg gcattgattc gttggcatcg tcatcgacaa
    30001 actccttacg cgctcaacag ttgcttcatg tcttcttcaa cagtggtgat agaacgcagg
    30061 ccgaatttct cgttcagcac cgccagcagg tctggtgtca ggaaaccagg tgcagtcggg
    30121 ccggtgacga tatttttcac gcccagagaa agcagcgtca gcagaatgac gatcgctttc
    30181 tgttcaaacc aggagagcac cagcgacagc ggcagatcgt tcacaccaca gcccagttta
    30241 tctgccagag tgacagccag aataatcgct gagtaagcat cgttacattg acctgcatct
    30301 accagacgcg gcagaccttc gatatcgcca aactcaagtt tgttaaagcg atatttacca
    30361 caggcgaggg tcaggatcag gcagtcatcc ggcacgatgg tggcgaaatc ggtgaagtag
    30421 tggcgctcgc cgcgtgcgcc gtcacagcca ccaagcagga agatatgacg cagtttttca
    30481 cggctcacca gatcaatcag cgtatcagca gcgccaagca gcgtctggcg accaaaaccc
    30541 acggtgataa ggtgcggaat ttcgctgtac gggaagcctg ccatctgttg cgcctgggtg
    30601 ataaccgcag agaaatcatc accgtccaga tgacgcacgc caggccagcc aacaatgctg
    30661 cgggtccaga tacgatcgtc ataagcgctt acggttgggt cgatgatgca gttcgaggtc
    30721 atcacgatgg ggccagggaa acgagcgaac tccacttgct gattctgcca gccgctgccg
    30781 tagttaccga ccagatgctt gaatttacgc agctccgggt agccatgcgc aggcagcatt
    30841 tcgccgtggg tgtagacatt aacgcccgtg ccttcggtct gttccagcag gttgtagaga
    30901 tctttgagat cgtgaccgga aatcagaatg catttacccg ccgtcgcttt gacgttgacc
    30961 tgggttggcg tcgggtgacc gtatttaccg gtttcgcctg catccagaat gctcatcact
    31021 ttgaagttca tctggccgat ttccattgaa cactcaagaa gcgcgttcat atcggcaggc
    31081 caggtcccca gccacgccat gattttatgg tactgggcat aaatatcgtt gtcgtattga
    31141 ccgagaacat gcgcgtgttc catataggcc gccgcacctt tcaggccata caggcacagc
    31201 agacgcaggc cgagaatgtt ttcgccaatc gccgctttat ctttgttagg ggtaaattct
    31261 gctgcctgac gttgcagctc gccgagatca tcgctcacca gttgcaggtc agccatcggg
    31321 ttatcgacgc gcgcgttggc atctacagcc aggcattgtg ctttcagcgc ctcgcgcaag
    31381 gcaatcgctt cacgagcgta gccgacaata cgcggagaat cgaagttaac gttggtcagg
    31441 gttgagaaaa aggcacgtgg cgcgaagctg tcaacatcgt ggttgatgat gccgtattca
    31501 cgcgctttta ccgcccaggc agaaagccct tgcagcgccg cgatgagtaa atcctgaagg
    31561 tcagaagttt ccgccgtttt accacacatc ccctgcgcgt atgagcagcc gtttcctgcc
    31621 ggagtacgga tagtttgttc acattgcaca caaaacatga tcacaccttt taaagttata
    31681 tttaatatac atgtttaagg ttaagacgct taacgcgggg ataaaaggga tttttcatgc
    31741 aactttaagg gagattgatt tagcgcaatt ttggcggcag ggatctaccg ccagagaggt
    31801 attacgcaga gaaaaaggcg atgaggatcg gcactaacag gctaagaata aaaccgtgaa
    31861 caattgccgc cgggaccata tccagcccgc cagtacgttg aagaacgggc agggtgaaat
    31921 ccattgatgt ggcaccgcat aagcccagtg cagtagagcg gctgcggcga atcagcccag
    31981 ggatcaacat aatagcaatc agttcacggg ccagatcatt aaaaaacgcc gcgctcccga
    32041 ttaccggacc aaaagattcg gtcaataaaa taccggaaag agaataccag ccgaaaccgg
    32101 aggccattgc cagcgcggta ttgatgggga gatcaagaat aaaggcgtta attaaaccac
    32161 caattaatga actgacaacc accaccacgg cgacaatcat tccccggcga ttaaggacaa
    32221 tctgctttaa ggtcatgcca ttattgcgca actgaatacc aacgaggaaa agtagcaaaa
    32281 ttaacgtgta ttcactggct tcggtcgcgt gttgtaagaa agccagtcca ctcagaccaa
    32341 tggcaaaacc aatcactact acgccgcaca gttttagcga ctccagcgcc atcgcaatac
    32401 gcgacgggag tttttcttgc tgatggtggt tgcgccacgg caggcctcgc tccagccaca
    32461 tcagggcggc aatattacac agtaaaataa cggtaatact gacggcagaa taatgcagaa
    32521 tcgccaacag gttactggcg aggttatcga gaaacgccag actgataccc ataaaaaaga
    32581 gaataaggta aaccatccag cttaatagct gattaataac ttttaacgca gcttgttggc
    32641 gaagcggaat gaggtaaccc acaatcaggg gaaccagaat gattaacagc ccagaaaaca
    32701 tgaaaaccca gtccttgcaa agatgaagtc gaaatgcgcg atgacacact actgaaagcg
    32761 gaaggacgag taaagttgca attaaaagga aatgttatgc ataaggagca gtagagtatt
    32821 cgttttcatt taaagatatt cttgcgcttt aattacaaac tgcaccgatg ttggtggcgt
    32881 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    32941 gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat aagtcgaggg
    33001 caccgcgcag cggcgatttt gttcgccaga gcccgggggg gcagggggcg gcggcgattg
    33061 gccgccccct gcgcgctcct tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc
    33121 gcaacaagat gatgaatcaa catataacaa catcttaaaa aaagcctgac attacgccag
    33181 gccttctgcg ttaattaatc acgcttttcc agcagggtcc ggtaaatcag accaccgata
    33241 atgccgccga caattggcac cacccagaag aaccacagtt gttctaatgc ccagccgccc
    33301 tggaagatag caaccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata
    33361 ctaattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa acctgccggc
    33421 gcgaatttgg cggttgcgcc gtggatcacc aacaggaaac ctgcactcaa taccagttca
    33481 actaccagcg cggaaagcat ggaataaccg cctggtgaat gctcgccata accgttagaa
    33541 gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa atacagcagc
    33601 gccgctgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc
    33661 ggaaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa
    33721 atatgaccaa cagcaaaggc catcgtcaga acggtcagac cgaacgccaa cgccacgccg
    33781 gcaaaaccaa tgcctaattc cgggaagcct gcggccagta cagcactacc acagccacca
    33841 aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca
    33901 attaaaaatt gaccctgtga aaaatatggt cgttttatag ggtcgtcgta aaaaaatgac
    33961 gacggaaata atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa
    34021 tccagtaaat ttgatttagg gcaacagcgg gttgccccat atagtcattt gtctgattga
    34081 cagtgtagtg cacgcaaaag atttaatcct ttaggcgtaa taaaaaataa tatatcatgc
    34141 taattatttg attttgttgt ttttgcagac ttatcagcaa gagggagtat aacgcgatta
    34201 ttcgctcatt tttcagacat ttgccatgct taaatgtgat gtcatcacgt attagcaagg
    34261 cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag gggatatgat
    34321 tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt tgatgctgga
    34381 acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc tggacgcctt
    34441 aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg atttctggtt
    34501 cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga ccttccgcga
    34561 atcgctgcca ggccgacatc gggttcgccg ttatcggccg ctggaagcgt gctggacgcc
    34621 atgcaccgat ggctatcacc gtatttttta tcgtctggaa ggggagagtg cggaagacgg
    34681 cagcgtgatg acactgcgca gttttctcga taaagacgga catccgattg atgtcgagga
    34741 tattaacgat caggcacgcc atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc
    34801 ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gtccctaatg tggaagtcac
    34861 cgcgcgccag ctcgatttcc tcgcccgtga gttatcctca catccgcaaa atctctctga
    34921 tgggcagatt cgtcagggac tttccgcaat ggtacagctg cttgagcatt atttctctga
    34981 gcagggggcc ggacaggcgc gatatcgttt aatgcggcgg cgggccagca atgagcaacg
    35041 aagctggcgc tatctggaca tcatcaaccg gatgattgac cgacctggag ggcgctcgta
    35101 tcgggttatt ttgctcggcc tgtttgctac tttattgcag gcaaaaggca cattgagact
    35161 ggataaagac gcgcgtccat tgttgctgat cgaagatcca gaaacccgtt tacaccccat
    35221 tatgctttca gttgcctggc atctgttgaa tcttctgcca ttgcagcgca ttgccaccac
    35281 caactcgggt gagttgcttt cgttaacgcc ggtagagcat gtttgccgac tggtacgtga
    35341 gtcctcgcgc gttgccgcct ggcgtctggg gccgagtggc ttgagtaccg aagatagccg
    35401 acgcatatcc tttcacattc gttttaaccg tccgtcatcg ctgtttgcac gctgctggtt
    35461 gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa ctggcgcgtc agtgcggaca
    35521 tcattttgat gccgaaggga tcaaggtcat tgagtttgcc cagtccgggc taaagccact
    35581 ggttaaattt gcccgccgaa tggggattga atggcatgta ctggtcgatg gcgatgaagc
    35641 agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg
    35701 agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt
    35761 ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctacgcaa
    35821 aattatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat
    35881 agaggcagga cgtcgtggtg tggactccgt accgacgctg ctgaaaaaaa tgttctcacg
    35941 cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg
    36001 ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat
    36061 ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca
    36121 tacgccgcca accgattccc agaaagcgtt gtaatcagcg tggatcttgc cttctttatc
    36181 gcgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac
    36241 ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg gaaatagccc
    36301 gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg
    36361 cagtccgcca ataaacatcg ttcttttccc ctgatattca cacagggtaa acgtgatctc
    36421 tgccagagga ataccttcgc tgttgcgaaa caggattgtg ctgtcacctt ctttatccat
    36481 tgagatcatc atggtcagct taagcgtgaa ctgctcgccg tttttgcctt ccagcttcgc
    36541 cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca tacatccgcg
    36601 gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc
    36661 aaggtaaggg cgatgcagac gcacaggcag tcgcggctgg cgcgttaaca acacattgag
    36721 attaggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggca taatcaacga
    36781 gcgcagcaaa aatttctggc gaaaactacg gcgatgccag aatttacccg gccgacactg
    36841 tccacgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt
    36901 ccgttcagtt agctgcgaca tattcatgaa atcattggtt atacatgacg tcgatttcac
    36961 cattgcgtat cttaaccaaa catcaatagt gtgattacta acgtaaattt tagggttttg
    37021 ttgatatttc gttgaagtta atgacccgga ttggcatatg gagtattcag aatatttatg
    37081 aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc tggtgatagt catcgccgga
    37141 ctgattacgt tatggagaat tcttaacgca cccgtgccga cttatcagac actgattgtg
    37201 cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag
    37261 gttgatgtgg gcgcgcaggt cagcggtcag ttgaaaactc tgtcggtggc gattggcgat
    37321 aaagtaaaaa aagaccagct tttaggggtt attgatcctg aacaggctga aaaccagatt
    37381 aaggaggtcg aagcaacgct gatggagcta cgtgcgcagc ggcagcaggc ggaagcggag
    37441 ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc tggcacaaac gcaggcggtt
    37501 tcacagcagg atctcgacac cgccgcgacg gagatggctg tgaaacaggc gcaaattggc
    37561 accattgacg cggaaatcaa gcgcaatcag gcttctctcg atacggctaa aaccaatctc
    37621 gattacactc gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac cactctgcaa
    37681 ggccagacgg tgattgccgc acaacaagca ccgaacattc tgacgctggc agatatgagc
    37741 accatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa
    37801 aaagcttggt ttacggtgct tggcgatcca ctgacgcgct acgaggggca aatcaaggat
    37861 gtactaccga cgccggaaaa ggttaacgac gctattttct attacgcccg ttttgaagtc
    37921 cccaacccca atggtttgct gcggctggat atgactgcgc aagtgcatat tcagctcacc
    37981 gatgtgaaaa atgtgctgac gatccctctg tcggcgttag gcgatccggt tggcgataat
    38041 cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc
    38101 gcacgtaacg ataccgatgt tgagattgtc aaagggctgg aagcgggcga tgaagtggtg
    38161 attggtgagg ccaaaccagg agctgcacaa tgacgccttt gctcgaatta aaggatattc
    38221 gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc accctcgata
    38281 tttatgccgg tgagatggta gcgattgttg gcgcttcagg ttccggtaaa tcgaccctga
    38341 tgaatattct cggctgtctg gataaggcca ccagcggcac ctatcgcgtc gccggtcagg
    38401 atgttgccac actggacgcc gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta
    38461 ttttccagcg ttaccattta ctttcgcatt taaccgccga gcagaacgtt gaagtacccg
    38521 ccgtctatgc cggtcttgag cggaaacagc ggctgcttcg cgcccaggag ttactgcaac
    38581 gactggggct ggaagaccgt acagagtatt atccggcaca gctttcgggt ggtcagcaac
    38641 agcgcgtcag catcgcgcgg gcattgatga acggcggtca ggtaattctt gccgatgaac
    38701 caaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc
    38761 gcgatcgcgg gcatatggtg attatcgtca cccacgatcc gcaggtcgct gctcaggccg
    38821 agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag
    38881 tgaatgttgc tggcgggacg gagcctgttg tcaacacggt gtctggctgg cggcagtttg
    38941 tcagcggttt taacgaggcg ctgacgatgg catggcgggc gctggcagcg aataaaatgc
    39001 gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg
    39061 tggtgggtga cgccgccaaa caaatggtgc tggcggatat tcgttctatt ggtacgaata
    39121 ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc
    39181 tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg
    39241 ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgttgctgcc agtgccaatg
    39301 gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaagga aacaccttta
    39361 atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc
    39421 agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg
    39481 ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc
    39541 gtgtctggct accttacagc acgatgtccg ggcgagttat gggccagtcg tggctgaact
    39601 ccattactgt cagggtaaaa gaaggatttg acagcgccga ggcggaacag caactcacgc
    39661 gtttactttc actgcgccac ggaaagaagg atttctttac ctggaacatg gacggcgtct
    39721 tgaaaactgt tgaaaagacc acacgtactt tacaactgtt tctgacgctg gtggcggtga
    39781 tttcgctggt ggtgggcggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc
    39841 ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac
    39901 agttcctgat cgaagccgta ctggtttgct tggtcggtgg cgcgttggga ataacgctgt
    39961 cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt ggtttttcac
    40021 cgttggcact gctgctggcg tttctctgct cgacggccac cgggatttta tttggatggt
    40081 tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggtacga gagtaatttt
    40141 tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga
    40201 cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg
    40261 ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa
    40321 ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag
    40381 atgaaaccaa accctttggc attgttgaac cacttaacag tacccttttc catgcttcga
    40441 catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt
    40501 tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcgtt
    40561 tgacaggggc cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag
    40621 gaatgtgaca gatgtcgctg atgccaacga tagatgatag ttatctatca tgtggagtag
    40681 attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga
    40741 agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa
    40801 gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat
    40861 gattacactc cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta
    40921 gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgcggagtc
    40981 tttaccgccg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat
    41041 gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag
    41101 gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc
    41161 gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca
    41221 tctgcccggg aggcgctgga agcgtgttct gtggatttgg ttgcgctccg tcaggaactg
    41281 gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca
    41341 cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc
    41401 ggtcgcaatg aggtaaccgg tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg
    41461 caggcggcat atctgttgcg taaacatgaa gtcagccgtc tcgatgtggt gaactttatc
    41521 tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac
    41581 agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaatcag
    41641 cttgcgcgcg tgggcggaat cgacccactg attggtcgtg agaaggagct ggagcgtgct
    41701 attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc
    41761 ggtaaaaccg cgattgcgga aggtcttgcc tggcgaattg ttcagggcga tgtgccggaa
    41821 gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc gggcactaaa
    41881 tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaggacact
    41941 aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc agcgtctggt
    42001 ggccaggtcg atgcggctaa cctgatcaaa ccgttgctct ccagcggtaa aattcgcgta
    42061 attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg
    42121 cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg aagaaactgt tcaaatcatc
    42181 aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg
    42241 cgtgcagcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc ggataaagcc
    42301 attgacgtta tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag caaacgcaag
    42361 aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcgcg cattccagag
    42421 aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg
    42481 ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt
    42541 gcaggtttag gtcacgaaca taaaccggtt ggttcgttcc tgtttgccgg ccctaccggg
    42601 gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gctgctgcgc
    42661 tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg
    42721 ggatacgttg gttttgatca gggaggtttg ctgactgatg cggtcatcaa gcatccacat
    42781 gcggtgctgt tgctggacga aatcgagaaa gcgcatccgg acgtgttcaa tattctgttg
    42841 caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac
    42901 gtggtgctgg tgatgaccac caacgccggg gtacgtgaaa ctgagcgtaa atccattggt
    42961 cttatccacc aggataacag caccgatgcg atggaagaga tcaagaagat ctttacaccg
    43021 gaattccgta accgtctcga caacattatc tggttcgatc atctgtcaac tgacgtgatc
    43081 catcaggtgg tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt
    43141 tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca
    43201 atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac
    43261 gaactgctgt ttggttcgct ggtggacggc ggtcaggtca ccgtcgcgct ggataaagag
    43321 aaaaatgagc tgacttacgg attccagagt gcacaaaagc acaaggcgga agcagcgcat
    43381 taatctgatt gttaggtagg ttggtcaagt ccgtaatctc gaaagaggtt acggactttt
    43441 tgtttatggg gtggaggagg ttcagaccct ttttttaatg atgatggtaa gttgttgata
    43501 attagtgctg cgggaaggta aggataaaaa agggtgctgc aggagaatgg gatggttttg
    43561 ctttatttac aacgggctaa acgtgtagta tttgagttca ctgccgtaca ggcagcttag
    43621 aaattcacag gtaacatact ccacccgccc accatgttca ctgccgtaca gacagataaa
    43681 atgcgaaaaa aaagctcgca ctttcgtacg agctcttctt taaatatggc ggtgaggggg
    43741 ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac
    43801 tcggacacct caccaaattg ttttgctacc aaacctcatg ggtggcaacg gggcgctact
    43861 atagggagtt ggagtaaaac ggtcaagaag aattttaatg ataattattg tttgctcata
    43921 ctgtaaacaa gttgtgcagt atatctacat cgagacaagt tacggactta tacttccaaa
    43981 gtacttcata catatcacaa aataaaaagg ccggttaaac cgacctttta ctcgttcttt
    44041 ctcttcgccc atcaggcggt aaaacaatca gcgactacgg aagacaatgc ggcctttgct
    44101 caggtcgtac ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt
    44161 tttgcgcatt ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta actctacgcg
    44221 gaacatggta ttaggcaacg tttcaagaac ggtaccttgc atttcaatat tgtcttcttt
    44281 ggccatctaa tcctctgggg tatcactacc gtaatttgaa ccggcaagat aatgccgaag
    44341 ttctgtaaat aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg gcacattact
    44401 ccgaaaacac acggctaagc cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa
    44461 cgatgggcgt tacctgacgc gaaaaattcc ttatcggcag cggggtaatg agcgtaacca
    44521 actctgcgac cgcaattata acactctggg gagaaatgtg ccgaaaacat tcattcttgt
    44581 ggtgaaaaca agcatcgtgg tacccagaaa ttattcggca atcgtccgag gcgcatttga
    44641 ttgagataat taaggtaatc ccggcgggga atttcgcagg caccaagcga tgctgtgtga
    44701 tcgttaagga cctggcagtc gataagctta ccgccatgac cgataaattc ctcacagaat
    44761 accagaagcg ccgttttaga cgcattttcc atccggctga acatggactc gccacaaaat
    44821 agcgttccct gggccacgcc gtacataccg ccgacaagct catcttcacg ccagacttca
    44881 atggagtggg catggccgag ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg
    44941 atccaggttc cttcttcgcg atcgctggca cagccttcaa tgacctgacc aaaagcgtaa
    45001 ttcatcgtga cacgataggg cgagcgttta tgaaatcgct tcatactacg gctgatatgc
    45061 agtgattctg gccatagcac cgcgcgggga tcgggcgacc accagaggat ggggtcgcct
    45121 ggagaaaacc acggaaaaat accacgctgg taagccatta acaggcgcgc agggctaaga
    45181 tcgcccccaa gtgccagcag gccgttaggc tcacgtaatg cgccttccgg ggaagggaag
    45241 gctattgaat ggcgagaaag ctgaaccagg cgcatgaccg caaaactcca cgcaagtcgg
    45301 atcgttcaat aatagcttac aaaccctgct tgaactggta ataacgcccc tgtctggcaa
    45361 gcagctctgc gtgagtacct tgctcaataa tttgcccgtt gtccatcact attatttgtt
    45421 ggaaacgaga gagtccgcga agtcgatggg tgaccattaa caccgttttc tcacgcatca
    45481 tttctgcaag caattcaagg atctggcttt cggttgtggc atctaagcct tcggtaggtt
    45541 catccagcaa caccagtggc gcatcatgta acagcgcacg ggcgatagcc agacggcgca
    45601 gttcaccacc ggagagctgg cgtccgcctt cacctaacca actgttgaga cctgcatcct
    45661 cgagcagctt ctccagacca acgcgacgca agctctccgc cagagcctca tcactactgc
    45721 caggcgaggc gagtaacaga ttatcacgca gcgtggcgct aaacagatgc actcgctgag
    45781 gaacaacgct gatggtctgt cgtagagccg cttcattcag gctggctatg gggctatcgt
    45841 taagcaaaat ctcgccctgt tgcgggtccc aagcgcgggt cagcagttgt aacagtgttg
    45901 atttgccgca tccggttcgc ccgagaatcg caatatgttc cccggcgttt acctgaagag
    45961 aaatcccttt aagtgcctgt tgggattgct ccggataagt gaactgaaca tcccgtaatg
    46021 tcagcgaaac gcgatcggca acacgagttt gggtatcagg gaaggtgact tccggttttt
    46081 gatccgttaa atcagtgatc cgaacggcag aggcaatgac ttgccccaga tgctgaaatg
    46141 cacccgttac tggtgccagt gcttcaaacg cggctaacgc gcagaagaca aacagggcaa
    46201 ttaacgcgcc gggttgagca ttgccgccaa cgccgccaga cgccatccac agcatcagga
    46261 tcaccgctaa cgcgccaatg agcagcatta tcgcttgcga caatgcggtc agttcagatt
    46321 gacggcgttg cgcttccagc cattgaattt ctgtattctc tagttgcgtg cgataacgat
    46381 cgctggcacc aaaaatggtc agctcagctt gcccttgcag ccaggccgtc agttgttggc
    46441 gatactgtcc gcgaagatga gtcagatttt gcccggtgct ttttcccgca cgataaaaca
    46501 gcggtggcat caggaaaagc gtcagtaaca taatgccgcc cagcgtaaag gcgagggtga
    46561 aatcaaggaa acttaacccg attgtcacca tcataatcac cacaaaagcg cccaccagcg
    46621 gcgagataac gcgcaggtaa agatgatcga gcgtatcaac atccgccacc acgcgattga
    46681 gcaattcgcc ctgacgatag cgcgccagtc cggcagggga gaggggcaac aatttgctga
    46741 aggtgtaaat gcgcagatgc tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt
    46801 caaaataacg cccggcagta cgggtgattg cggcaccacg cacgcccgca gcgggtagca
    46861 tatagttgaa gctgtacagt ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc
    46921 cggaaagtgt caacagtccg atactggcga gcagcgtcac aattgccagc acaataccaa
    46981 gacttaacat ccatttatga cgtttataca gtgctagata gggtagcaaa gcgcgcattt
    47041 aaatctcctc ctgacgatgg gccagtaatg tggcgaatgg gccaccagcc acacttaatt
    47101 ccgcgtaacg tccttgctca ataatccggc catcctgcat aacccaaatg acatcccagt
    47161 cagcaagatc ttctaactgg tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat
    47221 tcagcgcctc cattacgcgc tgttcactgt gagcatcaag gctggcagcg ggttcatcca
    47281 acagtaatag cgaacaggga tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc
    47341 ccacggaaag gcgggcagcc tggtcgccaa caggcgtatc aacgccttgt ggcaggagcg
    47401 gtagaaactc gctgacccag gcgttatcca gcgctgcttg taattcatgt tcgctggcat
    47461 caggtcgcgc cagtagtacg ttatcccgta atgttgctgc cggtaattgt ggattttgcc
    47521 caacccagga gagatgttta cgccatgatt ctggtgataa atcgcgtaat tctatcccgt
    47581 tgattcgtag cgatccctga tatgagagaa aaccagaaag cgcgttcaac agtgaacttt
    47641 tacctgaacc gctgcgacca accaacacgg cacgttggcc tgctggcaaa gtaaagttca
    47701 gcggtccggc cagcgtttta ccttccggcg acgtgataaa cagatcctcg gcctcaatgg
    47761 tcaccggatc ggtcgatgct aattccacct cgccacactg cggatgggcg agcggggttt
    47821 ccataaacgt tttcagactg tcagctgcac caacagcctg ggctttagca tgataaaacg
    47881 taccgagatc gcgtaatggc tggaaaaact ctggcgcaag gatcagggcc agaaaacccg
    47941 cagccagcgt cacgccagta tcgtagtgac caaaatccag ctcgccaaga taggaaaaac
    48001 caaagtagac cgccaccaga gcaattgaca gcgaggtaaa aaattcgaga atgccggagg
    48061 ataaaaatgc cagccgtagc acttccattg tccgttggcg gaaatcttcc gaagcagaac
    48121 gaatactttc aatttcagct tcaccacgac caaaaatacg caatgtttcc atgccgcgca
    48181 ggcgatcgag gaaatgccca cttaagcgag caagagcgag aaagttacgt cggttagcat
    48241 cggcagcccc cattccaacc agcgccataa acaacggaat taacggtgca gtgcccagca
    48301 gaatgagcgc cgcagcccag tttgagggga agattgccac cacaatcagc aacggcaccg
    48361 acactgccag cgccatttgc ggcaggtagc gtgcatagta atcatgcata tcgtcaattt
    48421 gctcgagtac cagcgtcgcc cagctccccg caggtttacc ctgaatccac gctggccctg
    48481 cttgttgcag acggtcgaga acctgccggc ggatggcaaa gcggatatgc tgcccggcgt
    48541 gataacccac ccgttcgcgt aaccagacca cccatgcgcg cagtacaaag gtcagaacca
    48601 gtaacgtaaa gggaagcagc agggcttcac ggggaatatt ctccataatc atatgttgca
    48661 gaattcgcgc catgaaccag gcctgggcaa tgatcaatat gccgctcaca aagcccagca
    48721 gacgagaaat attcagccaa cgttgggaga tgacgctttg ctgttttaac cagcgggtta
    48781 actctttttg acgagattta ttcattgcac gcttagcagg tgagttatca gaattatttg
    48841 cagagcaatg ttacaacggg gaaaaaataa aggcgaccca tagtcgcaag gtgtcgcctt
    48901 ctttactttt gttactgatt tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg
    48961 ttccgcatca agtgctgcca tgcagcctgt accggccgaa gtaatggcct gacgataaat
    49021 atgatccatc acgtcacccg cggcaaagac gccaggaatg ctggtctggg tggcattacc
    49081 atgaataccc gactgtactt tgatgtagcc gttttccagt tccagctgcc cttcgaaaat
    49141 ggcagtattc gggctgtgac cgatagcaac aaacagaccg gcaacgtcga gtgattcgat
    49201 gttatcgctg ttttgcgtat cgcgcagacg aacgccagtg acacccattt gatcgccggt
    49261 cacttcttcc agcgtccggt tggtgtgcag aatgatgttg ccgttctcca ctttatccat
    49321 cagacgctta atgaggattt tttccgcgcg gaaaccgtca cggcggtgaa tcagatgtac
    49381 ttccgaagcg atgttagaca gatacaacgc ctcttcaacc gcggtattgc cgccgccgat
    49441 gaccgcaact ttctggttgc gatagaagaa accgtcgcag gttgcacaag cagaaacccc
    49501 acggccttta aaggcttctt cagagggcag gccgagatag cgtgcagaag ctccggtggc
    49561 aataatcagc gcgtcgcaag tgtattcgcc gttatcgcca ttcagacgga acggacggtt
    49621 ttgcagatcc accttgttga tatgatcaaa aatgatttca gtttcaaatt tggtggcatg
    49681 ttcgtgcatg cgctccatta ataacggacc ggtcagatcg tttggatcgc caggccagtt
    49741 ttccacttct gtggtggtgg tcagttggcc gcctttttcc atgccggtaa tcagcacagg
    49801 ttgcaggttg gcgcgcgccg catagacagc agcggtgtat cccgccgggc ctgaacccag
    49861 gataagcagt ttactgtgtt tggtcgtgcc catgagatcc ccatagttgt tggcagacaa
    49921 tgggcaggat tgtagggaat ttactgacgt aaaaaaagag tatgacgatt ttgttaacaa
    49981 tttgtgcaat cggcagcatc gataagcagg tcaaattctc ccgtcattat cacctctgct
    50041 acttaaattt cccgctttat aagctgatta aatgatgaat aaacgcccct gttaatgaat
    50101 atctggcatg ttgtactaaa aatcgatgtt ttgctttgac aatcccctgg tgttttgcga
    50161 aaacattcga ggaagaaaaa aaacagtatt cttatatgcg cataaccatg catgtaaata
    50221 ccatgtttac cgtgctagtg aaatctacgt atggcgtgga cagacgccat tcgtgatgtc
    50281 gatagctgcc acaaggcaac ggtcttctca ccgtagaccc aggcattgcg cgccgtgaat
    50341 cttcatgatt tcggtctatc gtgacgggta gcgactctga acagtgatgt ttcagggtca
    50401 gacaggagta gggaaggaat acagagagac aataataatg gtagatagca agaagcgccc
    50461 tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat gagttgcaaa aggatgggcg
    50521 tatttctaac gtcgagcttt ctaaacgtgt gggactttcc ccaacgccgt gccttgagcg
    50581 tgtgcgtcgg ctggaaagac aagggtttat tcagggctat acggcgctgc ttaaccccca
    50641 ttatctggat gcatcacttc tggtattcgt tgagattact ctgaatcgtg gcgcaccgga
    50701 tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa gaaattcagg agtgtcattt
    50761 agtatctggt gatttcgact acctgttgaa aacacgcgtg ccggatatgt cagcttaccg
    50821 taagttgctg ggggaaaccc tgctgcgtct gcctggcgtc aatgacacac ggacatacgt
    50881 tgttatggaa gaagtcaagc agagtaatcg tctggttatt aagacgcgct aacacggaac
    50941 aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat ccatacagca acagtactgg
    51001 ggtaacctgg tactgttgtc cgttttagca tcgggcagga aaagcctgta acctggagag
    51061 cctttcttga gccaggaata cactgaagac aaagaagtca cattgacaaa gttaagtagc
    51121 ggccgtcgcc ttctggaagc gttgctgatc cttattgtcc tgtttgccgt ctggttgatg
    51181 gctgccttac taagctttaa cccttcggac cccagctggt cgcaaacggc ctggcatgaa
    51241 cctatccata atttaggtgg gatgcccggt gcgtggctgg cagatacgct gttctttatt
    51301 tttggcgtga tggcttacac cattcccgtc attattgtcg gcggttgttg gtttgcctgg
    51361 cgtcatcagt ccagtgacga atacattgat tattttgccg tttcgttacg catcattggc
    51421 gttttggcgc tcatccttac ctcctgtggt ctggcggcaa tcaacgctga cgatatctgg
    51481 tattttgcct ccggtggcgt cattggcagc ttactaagca ctacgttaca accactgcta
    51541 cacagtagcg ggggaactat tgcgctgctc tgcgtttggg ctgcgggcct gacgctgttc
    51601 accggttggt catgggtgac cattgctgaa aaactcggcg gctggatttt aaacattctc
    51661 accttcgcca gtaatcgtac ccgtcgcgat gatacctggg tcgatgaaga tgaatatgaa
    51721 gatgacgaag agtatgaaga tgaaaaccac ggcaaacagc atgaatcacg ccgtgcccgt
    51781 attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg aaaaattcat taatccgatg
    51841 gggcggcaaa cagacgctgc gttgttctcc ggtaagcgga tggatgatga agaagagatt
    51901 atctacactg cacgcggtgt ggctgccgac ccggacgacg tcctattttc gggcaatcgt
    51961 gcaacgcagc cagaatatga cgaatacgat ccattattaa acggtgcgcc aattaccgaa
    52021 cctgttgctg tagcagctgc tgctaccacg gcgacacaaa gctgggctgc gccggttgaa
    52081 cctgtgaccc agacgccgcc tgttacttct gttgatgttc caccttcgca acctacagta
    52141 gcctggcagc ctgtaccggg tccacaaacg ggagagccgg ttattgctcc tgcaccggaa
    52201 ggttacccac agcagccaca atatgcgcag cctgcagtgc aatataatga gccgctgcaa
    52261 caacctgtac agccacagca gccgtattat gcgcctgcag ctgaacaacc tgtgcaacaa
    52321 ccttactacg ctcctgcggc ggaacaacct gttcagcaac cgtattacgc aactgcgccg
    52381 gaacagcctg cgcaacagcc gtattatgcc cctgcgccag aacaaccggt ggcaggtaac
    52441 gcctggcaag ccgaagagca gcaatccact tttgctccac agtctacata ccagactgag
    52501 caaacttatc agcagccagc cgctcaggag ccgttgtacc aacagccgca acccgttgaa
    52561 cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc gcgtccgccg
    52621 ctttactatt ttgaagaagt ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc
    52681 tggtatcaac cgattccaga accggttaaa gaaccagaac cgatcaaatc ttcgttgaaa
    52741 gcgccttctg ctgcagcagt acctccagta gaagccgctg ccgctgtttc tccgctggca
    52801 tctggcgtga aaaaagcgac actggcgacg ggggctgccg caaccgttgc cgcgccagtc
    52861 ttcagtctgg caaatagcgg tggaccgcgt ccgcaagtca aagagggaat tggcccgcag
    52921 ttgccacgac cgaaacgtat tcgcgtgcca actcgtcgtg aactggcgtc ttacggtatt
    52981 aagctgccct cacagcgtgc ggcggaagag aaagcccgtg aagcccggcg caatcagtat
    53041 gattctggcg atcagtataa cgatgatgaa atcgatgcga tgcagcagga tgaactggca
    53101 cgtcagttcg cccagacaca gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc
    53161 gtaaacgcag aagatgcaga tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa
    53221 actcaacaac aacgttattc cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat
    53281 gattttgaat tttcgccaat gaaagcgttg ctggatgatg gtccacacga accgttgttt
    53341 acgccaattg ttgaacctgt acagcagccg caacaactgg ttgcaccgca gcagcagtat
    53401 cagcagccac aacagcaggt tgcgccgcag ccacaatatc agcagccgca acaaccggtt
    53461 gcaccgcagc agcaggatac cctgcttcat ccgctgttga tgcgtaatgg cgacagccgt
    53521 ccgttgcata aaccgacgac gccgctgcct tctctggatt tgctgacacc gccgccgagc
    53581 gaagtggagc cggtagatac ctttgcgctt gaacaaatgg ctcgcctggt ggaagcgcgt
    53641 ctggctgatt tccgtattaa agccgatgtc gtcaattact ctccggggcc ggttatcact
    53701 cgctttgaac ttaacctggc tcctggcgta aaagcggcgc gcatttctaa cttgtcacgg
    53761 gaccttgccc gttcactttc gacggtggcg gtgcgtgtcg ttgaagttat tcctggcaaa
    53821 ccctatgtag gtctggagtt accaaataaa aaacgacaaa ccgtttatct gcgcgaagtt
    53881 ttggataacg ccaaattccg cgataatccg tcgccattaa ccgtggtgct gggtaaagat
    53941 atcgccggtg agccggtggt tgccgatctg gcgaaaatgc cgcacttgtt ggttgcgggg
    54001 actaccggtt ccggtaaatc tgtcggtgtg aacgcgatga tcctgagcat gctttataaa
    54061 gcacagccag aagatgtgcg tttcatcatg atcgacccga aaatgctgga gctttcggtt
    54121 tatgaaggca ttccgcatct gttaacggaa gtcgttactg atatgaaaga tgccgccaac
    54181 gcgctgcgct ggtgtgttaa cgagatggag cgtcgttata aactgatgtc tgcgctgggt
    54241 gtgcgtaatc tggcgggtta taacgaaaaa attgctgaag ccgatcgcat gatgcgtccg
    54301 attccagacc cgtactggaa gccgggtgac agtatggatg cccagcatcc ggtgctgaaa
    54361 aaagaaccat acattgtggt gttggttgac gaatttgccg acctgatgat gacggtaggt
    54421 aaaaaagtgg aagagctgat agcacgtctg gcgcaaaaag cccgtgccgc gggtatccac
    54481 ctcgtactgg caactcagcg tccatcggtt gatgttatta ctggtctgat taaagcgaat
    54541 attccgaccc gtatcgcctt taccgtatcc agtaagattg actcacgtac cattcttgat
    54601 caggctggcg cggaatcact gctggggatg ggggatatgc tctactctgg gccgaactcc
    54661 acgttgccgg tacgtgtcca tggtgctttt gttcgcgatc aggaagttca tgccgtggtg
    54721 caggactgga aagcgcgtgg tcgcccacag tatgttgatg gcatcacttc cgacagtgaa
    54781 agcgaaggtg gcgcgggcgg tttcgatggc gctgaagaac tggatccgtt gttcgatcag
    54841 gcggtgcagt ttgtcactga aaaacgcaaa gcgtcaattt ctggcgtaca gcgtcagttc
    54901 cgcattggtt ataaccgtgc agcgcgtatt atcgaacaga tggaagcgca ggggattgtc
    54961 agcgaacagg ggcacaacgg taatcgtgaa gtgctggccc caccgccgtt tgactaatta
    55021 atgcattgcc ggataaggcg cggtagcgtc gcatccggca ctctatcaac tgaaaattca
    55081 gtattttctt ttttcctcaa gctgattatt agcctggaat agagagtaga gggaactccc
    55141 gatcgggagt gacgtaattt gaggaataat gatgaaaaaa attgccatca cctgtgcatt
    55201 actctcaagc ttagtagcaa gcagcgtttg ggctgatgcc gcaagcgatc tgaaaagccg
    55261 cctggataaa gtcagcagct tccacgccag cttcacacaa aaagtgactg acggtagcgg
    55321 tgcggcggtg caggaaggtc agggcgatct gtgggtgaaa cgtccaaact tattcaactg
    55381 gcatatgaca caacctgatg aaagcattct ggtttctgac ggtaaaacac tgtggttcta
    55441 taacccgttc gttgagcaag ctacggcaac ttggctgaaa gatgccaccg gtaatacgcc
    55501 gtttatgctg attgcccgca accagtccag cgactggcag cagtacaata tcaaacagaa
    55561 tggcgatgac tttgtcctga cgccgaaagc cagcaatggc aatctgaagc agttcaccat
    55621 taacgtggga cgtgatggca caatccatca gtttagcgcg gtggagcagg acgatcagcg
    55681 cagcagttat caactgaaat cccagcaaaa tggggctgtg gatgcagcga aatttacctt
    55741 caccccgccg caaggcgtca cggtagatga tcaacgtaag tagaggcacc tgagtgagca
    55801 atctgtcgct cgatttttcg gataatactt ttcaacctct ggccgcgcgt atgcggccag
    55861 aaaatttagc acagtatatc ggccagcaac atttgctggc tgcggggaag ccgttgccgc
    55921 gcgctatcga agccgggcat ctgcattcta tgatcctctg ggggccaccg ggtaccggca
    55981 aaacaactct cgctgaagtg attgcccgct atgcgaacgc tgatgtggaa cgtatttctg
    56041 ccgtcacctc tggcgtgaaa gagattcgcg aggcgatcga gcgcgcccgg caaaaccgca
    56101 atgcaggtcg ccgcactatt ctttttgttg acgaagttca ccgtttcaac aaaagccagc
    56161 aggatgcatt tctgccacat attgaagacg gcaccatcac ttttattggc gcaaccactg
    56221 aaaacccgtc gtttgagctt aattcggcac tgctttcccg tgcccgtgtc tatctgttga
    56281 aatccctgag tacagaggat attgagcaag tactaactca ggcgatggaa gacaaaaccc
    56341 gtggctatgg tggtcaggat attgttctgc cagatgaaac acgacgcgcc attgctgaac
    56401 tggtgaatgg cgacgcgcgc cgggcgttaa atacgctgga aatgatggcg gatatggccg
    56461 aagtcgatga tagcggtaag cgggtcctga agcctgaatt actgaccgaa atcgcaggtg
    56521 aacgtagtgc ccgctttgat aacaaaggtg atcgctttta cgatctcatt tccgcactgc
    56581 ataaatcggt acgtggtagc gcacccgatg cggcgctgta ctggtatgcg cgaattatta
    56641 ctgccggtgg cgatccgtta tatgttgcgc gtcgctgtct ggcgattgcg tctgaagatg
    56701 tgggtaatgc tgatccacga gcgatgcagg tggcaattgc agcctgggat tgctttactc
    56761 gcgttggccc ggcggaaggt gaacgcgcca ttgctcaggc gattgtttac ctggcctgcg
    56821 cgccaaaaag caacgctgtc tacactgcgt ttaaagctgc gctggcggat gctcgcgaac
    56881 gtccggatta tgacgtgccg gttcatttgc gtaatgcgcc gacgaaatta atgaaggaaa
    56941 tgggctacgg gcaggaatat cgttacgctc atgatgaagc aaacgcttat gctgccggtg
    57001 aggtttactt cccgcgggaa atagcacaaa cacgctatta tttcccgaca aacaggggcc
    57061 ttgaaggcaa gattggcgaa aagctcgcct ggctggctga acaggatcaa aatagcccca
    57121 taaaacgcta ccgttaatgt tatcgttgcg gtaatgttgt tactgtatcc ctgtggtcgc
    57181 aggctgtggc cacatctccc atttaattcg ataagcacag gataagcatg ctcgatccca
    57241 atctgctgcg taatgagcca gacgcagtcg ctgaaaaact ggcacgccgg ggctttaagc
    57301 tggatgtaga taagctgggc gctctcgaag agcgtcgtaa agtattgcag gtcaaaacgg
    57361 aaaacctgca agcggagcgt aactcccgat cgaaatccat tggccaggcg aaagcgcgcg
    57421 gggaagatat cgagccttta cgtctggaag tgaacaaact gggcgaagag ctggatgcag
    57481 caaaagccga gctggatgct ttacaggctg aaattcgcga tatcgcgctg accatcccta
    57541 acctgcctgc agatgaagtg ccggtaggta aagacgaaaa tgacaacgtt gaagtcagcc
    57601 gctggggtac cccgcgtgag tttgactttg aagttcgtga ccacgtgacg ctgggtgaaa
    57661 tgcactctgg cctcgacttt gcagctgcag ttaagctgac tggttcccgc tttgtggtaa
    57721 tgaaagggca gattgctcgc atgcaccgcg cactgtcgca gtttatgctg gatctgcata
    57781 ccgaacagca tggctacagt gagaactatg ttccgtacct ggttaaccag gacacgctgt
    57841 acggtacggg tcagctgccg aaatttgctg gcgatctgtt ccatactcgt ccgctggaag
    57901 aagaagcaga caccagtaac tatgcgctga tcccaacggc agaagttccg ctgactaacc
    57961 tggtgcgcgg tgaaatcatc gatgaagatg atctgccaat taagatgacc gcccacaccc
    58021 catgcttccg ttctgaagcc ggttcatatg gtcgtgacac ccgtggtctg atccgtatgc
    58081 accagttcga caaagttgaa atggtgcaga tcgtgcgccc ggaagactca atggcggcgc
    58141 tggaagagat gactggtcat gcagaaaaag tcctgcagct gctgggcctg ccgtaccgta
    58201 aaatcatcct ttgtaccggc gacatgggct ttggcgcttg caaaacctat gacctggaag
    58261 tatggatccc ggcacagaac acctaccgtg agatctcttc ctgctccaac gtttgggatt
    58321 tccaggcacg tcgtatgcag gcacgttgcc gcagcaagtc ggacaagaaa acccgtctgg
    58381 ttcataccct gaacggttct ggtctggctg ttggtcgtac gctggttgca gtaatggaaa
    58441 actatcagca ggctgatggt cgtattgaag taccagaagt tctgcgtccg tatatgaacg
    58501 gactggaata tattggctaa tacccaattt ttctgaatct aaaaagcgcc tgcgggcgct
    58561 ttttttgtct ccctttgata ccgaacaata attactcctc acttacacgt aatactactt
    58621 tcgagtgaaa atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata
    58681 tagcgagctg ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgagc
    58741 aagcttgagt gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggtg
    58801 agtcgccgtg gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca
    58861 ttaacattac cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca
    58921 gacgaaaagg ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt
    58981 atgcacgtcg tggacggtga aatcaaatat gtcgaaacgg acaataccgg cgatgacaat
    59041 tacgacggcc tgcactaggt tcgcgcctgc ctgcgtgggc gttccatgcg tcgccgtgtc
    59101 tacaatccgg atcgcctgaa atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa
    59161 ttcgagcgca ttagctggga agaagcctac gacatcatcg cgaccaatat gcagcgcctg
    59221 atcaaagagt acggcaacga gtctatctat ctgaactatg gcaccggtac gctgggcggc
    59281 accatgaccc gctcctggcc gccgggaaat accctggtcg cgcggctgat gaactgctgc
    59341 ggcggctatc tgaaccatta cggcgactac tcctccgcgc aaattgcgga aggtttgaac
    59401 tatacctacg gcggctgggc agatggcaac agcccgtcgg atatcgaaaa cagtaagctg
    59461 gtagtgctgt ttggtaataa ccctggcgaa acgcgaatga gtggcagtgg ggtgacttac
    59521 tatcttgaac aggcacgcca gaaatctaat gcccgcatga tcatcatcga tccgcgctat
    59581 accgacaccg gtgccgggcg cgaagatgag tggatcccta ttcgtccggg aacagatgcc
    59641 gcactggtta acggtctggc gtacgtcatg atcactgaaa acctggtgga tcaggcattc
    59701 ctcgataaat attgcgttgg ctacgatgag aaaaccctgc cagccagtgc gccgaaaaat
    59761 ggccactata aagcttatat tctgggtgaa gggccagatg gcgtggctaa aacaccggaa
    59821 tgggcctcac aaatcaccgg tgttccggca gacaaaatca tcaaactggc tcgtgaaatc
    59881 ggcagtacca aaccggcgtt tatcagccag ggatggggcc cgcagcgtca cgctaacggt
    59941 gaaatcgcaa cccgtgctat ctcgatgctg gcgattctga ccggtaacgt tggtattaac
    60001 ggaggcaaca gcggcgcgcg tgaaggttca tacagcttac cgtttgtccg tatgccgacc
    60061 ttggaaaacc cgatccagac cagcatttcg atgtttatgt ggaccgatgc cattgaacgt
    60121 ggcccggaaa tgacggcgct gcgtgatggt gtacgcggga aagataagct ggatgtgccg
    60181 atcaaaatga tctggaacta tgccggtaac tgcctgatta accagcattc tgaaatcaac
    60241 cgtacccatg aaatccttca ggatgataag aagtgcgagc tgattgtggt tatcgactgc
    60301 cacatgacct catcggcgaa atatgctgac atcctgctgc ctgactgcac cgcttccgaa
    60361 cagatggact ttgcgctgga tgcatcctgc gggaatatgt cttacgtgat tttcaacgat
    60421 caggtgatta aaccgcgctt tgaatgtaag accatctatg aaatgaccag cgaactggca
    60481 aaacgtcttg gcgttgagca acagtttact gaaggccgta cacaggaaga gtggatgcgg
    60541 catctgtatg cccagtcgcg ggaagcgatt cctgaactac caacgtttga agagttccgc
    60601 aagcagggga tctttaaaaa gcgcgaccca caagggcacc acgttgctta taaagctttc
    60661 cgtgaagatc ctcaggcaaa ttcactgacc acgccatcgg gcaaaattga gatttattcg
    60721 caggcgctgg ctgacattgc cgctacctgg gaattgccag aaggagatgt gatcgatcca
    60781 ctgccgatct acacgccggg ctttgaaagt tatcaggatc cgttgaacaa acagtatccg
    60841 ctgcagctta cgggtttcca ctataaatct cgcgttcact caacttacgg cgacgttgat
    60901 gtgctg
//
LOCUS       DAJTYD010000006        57063 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTYD010000006 DAJTYD010000000
VERSION     DAJTYD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57063
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.006"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..582)
                     /gene="wcaD"
                     /locus_tag="OO585_000352"
     CDS             complement(<1..582)
                     /gene="wcaD"
                     /locus_tag="OO585_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6628055.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQF"
     gene            complement(557..1774)
                     /gene="wcaC"
                     /locus_tag="OO585_000353"
     CDS             complement(557..1774)
                     /gene="wcaC"
                     /locus_tag="OO585_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6628056.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1771..2243)
                     /gene="wcaB"
                     /locus_tag="OO585_000354"
                     /pseudo
     CDS             complement(1771..2243)
                     /gene="wcaB"
                     /locus_tag="OO585_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            complement(2246..3085)
                     /gene="wcaA"
                     /locus_tag="OO585_000355"
     CDS             complement(2246..3085)
                     /gene="wcaA"
                     /locus_tag="OO585_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6628057.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGH"
     gene            complement(3263..5425)
                     /gene="wzc"
                     /locus_tag="OO585_000356"
                     /pseudo
     CDS             complement(3263..5425)
                     /gene="wzc"
                     /locus_tag="OO585_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            complement(5428..5871)
                     /gene="wzb"
                     /locus_tag="OO585_000357"
     CDS             complement(5428..5871)
                     /gene="wzb"
                     /locus_tag="OO585_000357"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6628058.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5877..6891)
                     /locus_tag="OO585_000358"
                     /pseudo
     CDS             complement(5877..6891)
                     /locus_tag="OO585_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
     gene            7200..7349
                     /locus_tag="OO585_000359"
     CDS             7200..7349
                     /locus_tag="OO585_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628059.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7550..9133
                     /locus_tag="OO585_000360"
     CDS             7550..9133
                     /locus_tag="OO585_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6628060.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(9407..11260)
                     /gene="asmA"
                     /locus_tag="OO585_000361"
     CDS             complement(9407..11260)
                     /gene="asmA"
                     /locus_tag="OO585_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6628061.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11282..11863)
                     /gene="dcd"
                     /locus_tag="OO585_000362"
     CDS             complement(11282..11863)
                     /gene="dcd"
                     /locus_tag="OO585_000362"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6628062.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11955..12596)
                     /gene="udk"
                     /locus_tag="OO585_000363"
     CDS             complement(11955..12596)
                     /gene="udk"
                     /locus_tag="OO585_000363"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6628063.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12914..16211
                     /locus_tag="OO585_000364"
                     /pseudo
     CDS             12914..16211
                     /locus_tag="OO585_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(16250..17107)
                     /gene="alkA"
                     /locus_tag="OO585_000365"
     CDS             complement(16250..17107)
                     /gene="alkA"
                     /locus_tag="OO585_000365"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6628064.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            17241..18593
                     /gene="yegD"
                     /locus_tag="OO585_000366"
     CDS             17241..18593
                     /gene="yegD"
                     /locus_tag="OO585_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6628065.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(18605..20527)
                     /gene="yegI"
                     /locus_tag="OO585_000367"
     CDS             complement(18605..20527)
                     /gene="yegI"
                     /locus_tag="OO585_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6628066.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(20524..21285)
                     /gene="pphC"
                     /locus_tag="OO585_000368"
     CDS             complement(20524..21285)
                     /gene="pphC"
                     /locus_tag="OO585_000368"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6628067.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(21282..21941)
                     /locus_tag="OO585_000369"
     CDS             complement(21282..21941)
                     /locus_tag="OO585_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6628068.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(22162..22221)
                     /gene="ibsA"
                     /locus_tag="OO585_000370"
     CDS             complement(22162..22221)
                     /gene="ibsA"
                     /locus_tag="OO585_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6628069.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(22494..22550)
                     /locus_tag="OO585_000371"
     CDS             complement(22494..22550)
                     /locus_tag="OO585_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6628070.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(22822..22878)
                     /locus_tag="OO585_000372"
     CDS             complement(22822..22878)
                     /locus_tag="OO585_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6628071.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            23157..24404
                     /gene="mdtA"
                     /locus_tag="OO585_000373"
     CDS             23157..24404
                     /gene="mdtA"
                     /locus_tag="OO585_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6628072.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGNRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            24404..27526
                     /gene="mdtB"
                     /locus_tag="OO585_000374"
     CDS             24404..27526
                     /gene="mdtB"
                     /locus_tag="OO585_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6628073.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLATLDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            27527..30604
                     /gene="mdtC"
                     /locus_tag="OO585_000375"
     CDS             27527..30604
                     /gene="mdtC"
                     /locus_tag="OO585_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6628074.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRAPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            30605..32021
                     /locus_tag="OO585_000376"
                     /pseudo
     CDS             30605..32021
                     /locus_tag="OO585_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            32018..33421
                     /gene="baeS"
                     /locus_tag="OO585_000377"
     CDS             32018..33421
                     /gene="baeS"
                     /locus_tag="OO585_000377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6628075.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            33418..34140
                     /gene="baeR"
                     /locus_tag="OO585_000378"
     CDS             33418..34140
                     /gene="baeR"
                     /locus_tag="OO585_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6628076.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            34331..34663
                     /locus_tag="OO585_000379"
     CDS             34331..34663
                     /locus_tag="OO585_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6628077.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            34786..36186
                     /locus_tag="OO585_000380"
     CDS             34786..36186
                     /locus_tag="OO585_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCR6628078.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            36507..37868
                     /gene="yegQ"
                     /locus_tag="OO585_000381"
     CDS             36507..37868
                     /gene="yegQ"
                     /locus_tag="OO585_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6628079.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(38198..38515)
                     /locus_tag="OO585_000382"
     CDS             complement(38198..38515)
                     /locus_tag="OO585_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628080.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            38929..39827
                     /gene="yegS"
                     /locus_tag="OO585_000383"
                     /pseudo
     CDS             38929..39827
                     /gene="yegS"
                     /locus_tag="OO585_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(39909..40682)
                     /locus_tag="OO585_000384"
     CDS             complement(39909..40682)
                     /locus_tag="OO585_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6628081.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(40788..41828)
                     /gene="gatD"
                     /locus_tag="OO585_000385"
     CDS             complement(40788..41828)
                     /gene="gatD"
                     /locus_tag="OO585_000385"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6628082.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITIGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(41876..43231)
                     /locus_tag="OO585_000386"
     CDS             complement(41876..43231)
                     /locus_tag="OO585_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6628083.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(43235..43519)
                     /gene="gatB"
                     /locus_tag="OO585_000387"
     CDS             complement(43235..43519)
                     /gene="gatB"
                     /locus_tag="OO585_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6628084.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(43550..44002)
                     /gene="gatA"
                     /locus_tag="OO585_000388"
     CDS             complement(43550..44002)
                     /gene="gatA"
                     /locus_tag="OO585_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6628085.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(44012..45274)
                     /gene="gatZ"
                     /locus_tag="OO585_000389"
     CDS             complement(44012..45274)
                     /gene="gatZ"
                     /locus_tag="OO585_000389"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6628086.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(45303..46157)
                     /gene="gatY"
                     /locus_tag="OO585_000390"
     CDS             complement(45303..46157)
                     /gene="gatY"
                     /locus_tag="OO585_000390"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6628087.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(46465..47517)
                     /gene="fbaB"
                     /locus_tag="OO585_000391"
     CDS             complement(46465..47517)
                     /gene="fbaB"
                     /locus_tag="OO585_000391"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6628088.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            47774..49051
                     /locus_tag="OO585_000392"
     CDS             47774..49051
                     /locus_tag="OO585_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6628089.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            49048..50052
                     /locus_tag="OO585_000393"
     CDS             49048..50052
                     /locus_tag="OO585_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6628090.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            50049..51014
                     /locus_tag="OO585_000394"
     CDS             50049..51014
                     /locus_tag="OO585_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6628091.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(50988..51734)
                     /locus_tag="OO585_000395"
     CDS             complement(50988..51734)
                     /locus_tag="OO585_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6628092.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(51786..52604)
                     /locus_tag="OO585_000396"
     CDS             complement(51786..52604)
                     /locus_tag="OO585_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6628093.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQKVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(52669..53469)
                     /gene="thiD"
                     /locus_tag="OO585_000397"
     CDS             complement(52669..53469)
                     /gene="thiD"
                     /locus_tag="OO585_000397"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6628094.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(53466..54254)
                     /gene="thiM"
                     /locus_tag="OO585_000398"
     CDS             complement(53466..54254)
                     /gene="thiM"
                     /locus_tag="OO585_000398"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6628095.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(54289..54385)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(55966..56259)
                     /locus_tag="OO585_000399"
     CDS             complement(55966..56259)
                     /locus_tag="OO585_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628096.1"
                     /translation="MYGQDTTAGGNLKKDSPLGKLWDFLHSPDLPTNPDFAKPADKTG
                     WVSSYSNPTYVPPTPTITAPQLKVDMAGNPLMTPPAWLNQPLNVNVNVKVQDG"
     gene            complement(56223..>57063)
                     /locus_tag="OO585_000400"
     CDS             complement(56223..>57063)
                     /locus_tag="OO585_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6628097.1"
                     /translation="VGLFVKALNQMKGTTNLTEKDLFDLMQQGKLFSKDILPEVAKQM
                     KAAARNGGALDKAMKSNRASWQRLQTSMQNAMNIFFASGFGNELTSAFDAISAAVNGS
                     GGAFEMFGHIAGKIVEGATDAFTELHDTVILTFRIIEYYASKMGITGEQLKAWGEMAA
                     YAAGVAMFAGSIWKLGVAFKWLLTMLNPLTKLLGVMKGIAAIGGIEAATTGGSGGEGK
                     DKPNKPGRGPKVGKVGGAAALSGGPVGWSIAVGSVGGDYAWDKVMTLTTPCMDRTPPP
                     GAI"
BASE COUNT        13573 a        14976 c        14427 g        14087 t
ORIGIN      
        1 aaactgtttg atgctgagcc aaattgagat taatgccaga gcgaaaaatg ccggttcgaa
       61 ataaagtgct gtcgtgcgct tgccgccgaa tttaatgaaa ttcagcacat agctgttgct
      121 gtaaatcaga tatttcgaaa tcgactccat aatactgctg ccaccggtga ggataatttg
      181 cgccatctcc actgccgcca gcgccaccac cagccctacc accagataaa agaaacgtaa
      241 tatcttgcga tggttgtgtg gcgaaattgt tttaaaacga atactccaca ccatgccaat
      301 aatgatcaca atatagacaa acagcatggt tgaagtgacg tatttactgg catccagcga
      361 ctgaccaaac aggtagttaa acgccgtgag tcccgcacca atccctaagg caatcatcaa
      421 ttttttaacg ctgatgcgtt ctaaaaacag cagtaacaag acaggtaaaa aagtgacgat
      481 agtgatgggg aaactttcac caagctgggc gattttgacg ttaaccagca ggtagatcag
      541 cggcagcagc aggtagctac agattctgat agaagttgac atactcctcc agcatctgtt
      601 gtccactgta ggcggcgcgg ctgcgctggc tgaacccggc caacgtggta ccaaatatcg
      661 cctgcgcgat ttccggtttg cttaattgca ctagttgcag cacctcttct tcgctgacgg
      721 ttttaccgcc ggatttttgc aacacttccc gcgccgcgtc gctatgggtg gcaatcaccg
      781 gcacgccaat cgatagcgcc tcacacaaaa tcagcgggta gttatcgacg cgagaactga
      841 acaccagcgc atccatctga ttgagcgcgc tcatcaactt gcgcttgtca gtttcaaagc
      901 cgtgattaac cacgttgcca gcggtgaacg gcgagaactt accaaaggta tgcagttcga
      961 ttttgtcgcc cagcgccatc atctcacgca ccagttgctg gttagtcttg ccgtcgtaac
     1021 gcaagtcatg cgccaccacc gcgattttcg gcttgccctg ggtttcgcgc accggaggca
     1081 agtccgccag aatcgcttcg gttgccatat caataccatt attgataatc cggcaacgcc
     1141 ctggaccgta caggctattg aaagcgtcag ccacatgctg gctgggggaa ataaactgac
     1201 agcccagcgc cagcatctca cggaataact ggcgtttgcc cgccaccagc tggtgtgcgc
     1261 gatcaatctt caccggcgga tagttattca aggtcgggca tttctggcag cccgttttcc
     1321 agccttcgca accgtcggta aaggcgcagc gtccggtaac actccagtgg tcgtgcagcg
     1381 tccagaccag agtgacgtcc ggtttatggt ttttcacttt ttcgcaaaag cgcaccacgc
     1441 tcttaagatt tagccagtag ctgtgcagca catgaaaatg caggaccacc ggacccggtg
     1501 tgcgagtaat ggtgcgatat aactcattga aattgccaaa cagatcgcga ttaaacagac
     1561 gaaacagggc aatattcgcc atcgcggtca tccgcggcgt atgtttgatg acctgcggat
     1621 agttctgatg gctgacgctc tctttgccgc ctttgccgta accgtagaca aaatgtgacg
     1681 ccagcccctg ttgcagcgca cgctggtgga gatctaacgc cacacctgct gccccgcctt
     1741 ccgccagtcg cacattaaat tgcagaatat tcatttaatt accttcactc gcgctttttc
     1801 tcctaccacc agcgcgttgt ccgggacaga gtcgagcacc acgctgcccg cacccacggt
     1861 gacgttgtta ccaagcgtga tatcaccaag aataatgacg ttggcaccga gttcgacgcc
     1921 gttgccaatg tgtggacatg ccatgttatc ggcaccacga ttgccgatag tgacgccgtg
     1981 gcgaatggtg aaatcatccc ccgctaccac gtttttattg atcactacgg cgtaaccgtg
     2041 atggatagta aagcggcggc ctggatttca taaccgaaaa agcattcggt gataatgcga
     2101 tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt gcgccacacc
     2161 gaacaaaaat gagcaacacg ataggcaaga accatgcagc acgggcgtaa actccagctg
     2221 ttggcacgca gatcttccag catctttagt gcccccgaat accatcagcc agacgtttgc
     2281 cgttacgcac cgacagcagc gtcagcaaag tgcgccaggt catacgttta ttgcggatct
     2341 ggtagagggt aaacagctga tattttttgc ttgcacggtc aaatttatct ttgtgctttc
     2401 ggtaaaagtg gaagtacccg gagaattttt tcggtgacga ggtgatctgc atttcaccgt
     2461 gattgatatg caggatctgc gtcgcttctt ccactttcca cggttcgccg tactccacca
     2521 ccatccgcag gaagatgtcg taatcctgtg ctgctttcag ttcggtatcg aacaaacact
     2581 ctttgaaacg ccatgcccaa gtaaagacct gattgccaat gatattgcgt ttatagaaca
     2641 ggcggcgtga atacggcgat ttgggatata gcggcaaact tgctggttgg gaatagactt
     2701 cgccctggca aacgtagtcg ttagcgtaca aaaaggcgtg agtgaccagt tgctgtttat
     2761 gggcgaggaa gacgctcaga cggttgggtg tccattcgtc atcgtcatca atcccggtga
     2821 tgtattcccc ttgcgccagc ataatcgcct ggttacgtac cgcgcacgcc ccgctgttaa
     2881 tatcgttgtg gatgtaagtg atacgcggat cgttgagggc ggtgacgtac tgttgcagtt
     2941 gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg tagtcctggc
     3001 gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtc ggcatataga
     3061 ttgagatcag cggattgtct ttcataggtt gttctcccgg agtgtcgaga tgttctgccg
     3121 gtttataagc ttgtttacgg gttctgttgc cggatgcggc gtgagcacct tatccggcct
     3181 acggagctgg tttgcttgcc ggatgcggcg tgaaggtagg tcggataaga tgcgtcagca
     3241 tcgcatccga cagcggatgt cgttatttcg catccgactt atattcgtat tcgtaatagc
     3301 cataatcctg atacgcgctg gcgcggcgga agatggagtt cagaatcacc cctttcaccg
     3361 gaataccgtt ttgctcaaag cggctcagac tggtttccac ttctttcaag gtgttgaccg
     3421 cataacgcgc caccattaac gtggttccga catgacgacc aacaattgcc gcatcggtca
     3481 ctgccagaat cggcggcgta tcaatcaaca ccaggtcata gtttttactc gcccagttca
     3541 ccagttcggc aaagcgttcg ctcatcaaca gttcagaagg atttggcggc acctgaccgc
     3601 gcgggatcag gtcaaatttg gcaatagagg tcggtttagc agcggtagta atatcgccct
     3661 gaccaatcag aatttccgac aggccattaa cgttattagt gcacagcagc tcgtgggtgt
     3721 agcctttgcg catatcgcag tcgatcaaca acacccgttt attggtctgg ctgatcaccg
     3781 ccgccaggtt ggcgcagaca aaggttttac cgattgacgg gctaaccccg gtcatcatca
     3841 acacattgtt ctgtgcctgc atcatcgcga agtgcaaact ggtacgcaga ctgcggatgg
     3901 cttcaatcgc cagatcggtt ggattcccca tcgccagtag ctggctctgt ttatagcgtt
     3961 taatcccttt gatggttttg acgctatcgc gcgctttctg ccattccgac agcgggatgc
     4021 tggcatagac gctgataccg tgttcttcca gcacctgcgg gctttcgatg ccgcgattaa
     4081 acaacgagcg cagcagcaca cccacgatag aaagcatcag gccaaggata atcgcgccga
     4141 ggataatcag ccctttcttc ggttttagca cgccaggctg ggtgattgcc gggtcaacaa
     4201 tgcgcacatc gccgacggtg ctggcttcgg tgattttcag ctcctgctct ttattcagca
     4261 gttgcatata gacctgctga ccagactcga catcgcgggt caggcgcaca atctcctgct
     4321 gggttttcgg catcgccgtt acgcgaccgt taagtttggc tttctcttct tccagcgcct
     4381 gacgtttctc cagcagcgtg cggtacgccg ggtgaacttt ggtgtacagc ttggagattt
     4441 ccgcctcttt aaaggtcagt tcgttcaact gcgcgtcgat gttcaccatc gaatcgagca
     4501 ccgctttcgc ttccagcggc agatcaacag aatctttatc ctgacggaag gcattcagtt
     4561 tgttttcggc aacatcgagg cggctacgta cttccggtaa ctgttgcgcg aggaaagcga
     4621 ggcttttcga cgcttccgcc gatttgcgct caatattttg ttcctgatag ttacgggcga
     4681 tgctgttaag aatgtcgcgg atctgttcgc gatcttcacc ggtataagtc aggctcagta
     4741 cgcctgcgtc tttgccgttc tccgttacca tcaggctgtt ttgcagttgg ttgatcatcc
     4801 ccaacgtgga gtatttggtg acggtaaact cactgcccgg gcgggcgtga atggcttcaa
     4861 ccatcagcgt gacgccttct tttttcagca tctggcccgc ttgcccacgg gcgctaaagc
     4921 cgccatcact gctcagggtg tagtttttat tgtccagcac attaagcgta aacacctagt
     4981 ccgccatctc tttcgggcgg ttaaaggtag tcactttcac cgtctcgttc tggcgtccca
     5041 tcagtcgatc ccagcccgcc ccgaaaatcg ggaacgtgtt tttgctcact gcaatatcga
     5101 ggtcgagatc gtccaccgtt ttaccaagca ccagacgcga acgaatcaac tggatctcgg
     5161 cgtccgatgc aggcggtttg ttggctaatg ccgaaccaat gtcctgcact aacgaattgc
     5221 cgctgttttg ctcgatttgt accagtgcgt cggcactata aatcggcgtg gcgaagaagg
     5281 tgtaaaccac ggcacagagg acaaatacag cggtgatgcc aatcacccac cagcgcgctt
     5341 caatgacggt gccgaccagg cgaccaatat cgatttcatc actgcccgtt accggagcgg
     5401 catgttgttt tactttttct gtcattctta tacctgctct gcgttcaatg cctgcgccca
     5461 ctggcgggca gaccgttcaa gtaatgtgta caccgctgca aacgtttccc agcttttgcg
     5521 atacggatcg gggatttcac attcgttatc ccagtgacca aacagcatca ctttgccgcg
     5581 catctcaggt gccatctcgc ataagcgttc gatatggcgc ttttccatgg tcaaaatcag
     5641 gtcatagttg cgacacaggc ggcggctgat ttgtcgtgca cagtggcctt ccagagacag
     5701 ttgatgttct gcggcgacgc tgatagcggt aggatcagcg cccttaccga ccagcgcgcc
     5761 gagtccagcg gactccactt tcagttccgg gtgataacgt tgcagtaagc gttccgccgt
     5821 cggggaacgg caaatattgc cgacacagac aactaatatg ttgttaaaca tgacgattac
     5881 cagttatgaa tgtcgctggc tgtatccgtc atgtaacgga caccgctaat agttggcaat
     5941 cttgccgtta cgcccgccct cgcctttcag cggacgaatg acaaagatgc cgctggcgtt
     6001 ggaggtggtc atgtcgatac cttcagcatt acccagggct tcagtcaggg cttcagtcag
     6061 ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa
     6121 tactttcaga tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt
     6181 ctgattgagg tcgccgtttt gcatcagcac ctgcaaagaa atgcgttctt cacgaccatt
     6241 gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat
     6301 ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg
     6361 acctgagata taggtctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc
     6421 agcgatgtac gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc
     6481 gactacgtga accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt
     6541 gccggtgtcg ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca
     6601 gacggtgaca ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct
     6661 ttccagcgtc atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg
     6721 cggggtcagc ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt
     6781 gatgacgtct ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat
     6841 caaggttact gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt
     6901 atcactttgg cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt
     6961 accttgcagt taattgaata caaatttaac tggcatcagt cctaaaaaaa ttgatttcat
     7021 ccgcaggcta ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc
     7081 ccctggcttt ttagccacca atacactgat ttagcttaat tttttaaacc ctctctgcat
     7141 acaatcgttg atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta
     7201 tggtaattga atattggctc tccaataatg caggaggaag tgttacagct aacggaatag
     7261 caggcaaggt aacaattcgg caattggcta tttttaagaa ttatattaag tgtcattcaa
     7321 tatggttttt aggagtttct ttaggttgac aatatttaat attgcgcctc cgcatgcgat
     7381 atttcttaaa taatgtttta ttattaccac ttttaattca gggataatgt gaggttatta
     7441 ccctcaaata atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca
     7501 tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat
     7561 tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg
     7621 cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga
     7681 ccgcgcacgg gttaccgggc tgttgctggc gatgttaatg cgcctgttac tgctggcgtc
     7741 aatctcctgg ctggtcaccc tgacgcaacc gctgttcagc ttccgctcgt ttacctttag
     7801 cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga
     7861 gctgaacgaa cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa
     7921 gttctggggg gtggtgacac aaattgtggt actggacgcc atcttctcac tcgactcagt
     7981 gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc
     8041 tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac
     8101 catcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg
     8161 tttcggcttc gtcattccga aaggctacct gtacgctgcc attggtttct cggtaatgat
     8221 cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaaccagac
     8281 gctacgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc
     8341 ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggtaaccagc agatctttaa
     8401 tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta ctgtcagcag
     8461 cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagacgagat
     8521 ccgccagttg ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc
     8581 agaagatttg ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgtggcga
     8641 accgctcaac ctgcgggtgt tgattcgcca gccgctagtg ttcccggaaa ccttgccgtt
     8701 gttacctgcc ctggagcagt tccgtaatgc ccgcacacac tttgcttttg tggtggatga
     8761 gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa
     8821 cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg
     8881 ttcctggacg gcgaatggtc atatgccgct ggaagatctg gtgcaatatg tgccgctgcc
     8941 gctggatgaa aaacgtgaat atcacaccat tgccgggctg ttgatggaat atttacagcg
     9001 tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt
     9061 ggaaagccat cgcgtgcaga aggtgcagat tattccgctg cataaggatg gcgagttgga
     9121 gtacgaggtg tgatggtatc ggtcatttta cccaatagcc gaaaggaata agatgaggga
     9181 accgcgccag cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc
     9241 ccccctggca cgttcaccgg cttgagcgtt ccatagtagc gaggaacatc aagtgaacgg
     9301 aacagccttc gatcttacat attcagaata taatgatgcc gccacgaacg cctgatgcga
     9361 cgctttacgc gtcttatcag gcctacagga caggtcacca ggtaacctac atcttctcca
     9421 gcaacttctt cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca
     9481 gacgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcagactgt
     9541 aattgagttg ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttaaggaaat
     9601 caatcagttt gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc
     9661 aggtttgctc cgccagattc aacgtaccag ccccggaaag cgccagcaca ggcgattgac
     9721 cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt
     9781 caagacgcgt tacgttatcg aagttttcag cggccttcac atcaccacca ttacgttcta
     9841 ccgcttgctg aatcatctgc tggaagttca tcccttccat gcgcgtgtcg gtcatttcga
     9901 catgtgcctg tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga
     9961 aatcaccagc cagtgacatc tttccggtca acgaaatcgg atagttaaac gccttcagaa
    10021 tggtgccaat ctcaacgttt tccagccgtg gctggaagtt tatccgcgga tttattgatg
    10081 tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt aagcttgccc tgcagttgag
    10141 taatttccag caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc
    10201 cgcgccagcg cacgttactg gcctgcaaca aaatatcagc cgtaaagcct tgcagtccct
    10261 gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg cggcaacgtt gattggctct
    10321 gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc attaagcggg atgaggttgt
    10381 caagattcag ttgcgggaat tgcagtctca gctgccattc cggtttctct gtcagcgtga
    10441 cctgtgcttg cccgctcagc gtactgtcat tggcggtcaa actaatttgg ttaaatgaaa
    10501 ggcgtttatg tgactcctgc cactgggcct ggaaactccc ctgcccatga ataccttgtt
    10561 ttggtaaatc tgcaccctgc aactgccagt taatttgttc aatagccgcc gttaaatcat
    10621 gcggataatc agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc
    10681 gattaacccg cccggagaac tcaaatgaac cacgatgttg gggatcttgt tccatttgca
    10741 ggcgaatatt gcggattgtc acctgctcgt catcttcatg ctggaacacc agcacgctgt
    10801 ccgccacctt aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca
    10861 aggtattgtc gcgcggtgca accggagcgt cttcactgcg caccacttcc gtctgcggcg
    10921 tcagttggat cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta
    10981 gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggctcgcgc
    11041 cctgggcggt gagagacatt cgcccagaga ggatactaag ctgcggccag acgtgccaac
    11101 gcagtggccc gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat
    11161 agttgcggaa atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca
    11221 cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga
    11281 attagtcttt atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt
    11341 cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa
    11401 ttaacatgcc cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa
    11461 tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac
    11521 gcgccaaaga ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact
    11581 ccagcgtcac cgccagcgcc agttctcctg ggtgaagata gaacgcctcg ccctcgtcga
    11641 gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc
    11701 tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaggcgta
    11761 catccaccgt cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc
    11821 cttcatcaag ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg
    11881 cgcatcactc ccttaacgcc aattacgtca agggcatacc aggttatcac tgaacggtac
    11941 acaatttatc aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg
    12001 atgcggtttt tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg
    12061 aattgcagga acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa
    12121 cgcccacgct cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta
    12181 tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg
    12241 ccttcgagaa tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt
    12301 tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc
    12361 tcaagcagca ggttgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct
    12421 tccatcgaca gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt
    12481 tcatcaccga cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg
    12541 gaagccgatg cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat
    12601 ttagcgacct gattaacctg gatgttagga agggggcgac gaagcgccaa acgcggcaat
    12661 tatagggatt tcatccgcct gataccagtc gaatagcgtt gccgcgcgct cagagttaat
    12721 tgttgacaaa gaattcccgg tggcaaatta cgttgatcag ttttatgtaa ggtaaaaaat
    12781 gttatacgca gttgcgcaaa ttatccgcct ttacgtcact ttatgagcaa ttcacatata
    12841 aaatgtaaaa cttttgtact agcataaaca cagaaacgaa tactggcgac ctggtcttgc
    12901 ggataaagcg gtaatgagca aacaatcaca gcatgtatta attgccctgc cccacccact
    12961 gcttcacctg gtcagtttag gtttagtctc gtttatcttt acccttttct cgcttgagct
    13021 ttcgcagttt ggcacccaac tcgccccact gtggttcccg acgtccatca tgatggtggc
    13081 gttttatcgc catgccgggc gcatgtggcc gggaattgcg ctgagctgct cgctgggaaa
    13141 tatcgccgca tccatcctgc ttttttccac cagctcgctg aacatgacct ggacgaccat
    13201 caatattgtt gaagccgtgg tcggggcagt gctgctacgt aaattgctgc cgtggtataa
    13261 ccccttgcaa aatctggctg actggctgcg tctggcattc ggcagcgcca ttgttccgcc
    13321 tctgttaggg ggtgttctgg ttgtcctgct gacgcccgga gacgatcctc tcagggcatt
    13381 tttgatatgg gtactgtcag aatccatcgg cgctctggta ctggtgccgc tgggattgtt
    13441 atttaagcca cactatctgc tgcgccatcg caacccacgg ttactttttg agtcactgct
    13501 cacattagcc atcacgctga cgttaagctg gctttctatg ttgtacctgc cgtggccttt
    13561 tactttcatt attgtgctgt tgatgtggag cgccgtgcgc ctgccacgaa tggaagcctt
    13621 tttgatcttc cttaccacgg tgatgatggt gtcgctgatg atggccgcgg atccctccct
    13681 gcttgctacg ccgcgtacgt acctgatgag ccatatgccg tggctactgt ttttgctgat
    13741 cctgctgccc gccaacatca tgaccatggt gatgtatgcc tttcgtgcgg aacgcaaaca
    13801 catttccgaa agcgaaaccc gctttcggaa cgcgatggaa tattccgcta tcggtatggc
    13861 gttagtgggc accgagggac aatggctgca aaccaacaaa gcgctctgcc agtttctcgg
    13921 gtacagtcag gaagagctgc gcggactcac ctttcagcaa ctgacctggc cggaggatct
    13981 caataaagat ctccaacagg ttgaaaagct gataagcggt gaaataaaca cctattcaat
    14041 ggaaaaacgc tactacaacc gtaatggcga tgttgtctgg gcgttgcttg ccgtctcact
    14101 ggtgcgccac acggatggca cgccgctcta ttttatcgct cagattgaag acattaacga
    14161 gctaaaacgc accgaacagg tgaatcagca actgatggag cgcatcacgc tggctaacga
    14221 agcgggcggg attggcatct gggagtggga gctgaagccg aatatttttc gagctgtatg
    14281 aaattcctcc gcatatcaaa ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag
    14341 atcgccagca tgccgaaaaa gtgattcgtg attcgttgca atcacgctcg ccctttaagc
    14401 tggaatttcg cattaccgta aaagacggta ttcgccatat ccgcgccctc gccaaccggg
    14461 tactgaataa agaaggcgaa gtcgaacgcc tgctcggcat taatatggat atgaccgagg
    14521 ttaaacagct taacgaggca ttgtttcagg aaaaagagcg cctgcacatt acgctggatt
    14581 ccatcggtga agccgtggtc tgtattgata tggcgatgaa aattaccttt atgaatccag
    14641 tggcggagaa gatgagcggc tggacgcagg aagaagcatt aggtgttccg ctcctgacgg
    14701 tgttgcatat tacttttggc gacaacggac cattaatgga gaacatttac agtgccgaca
    14761 cctcacgttc cgcgattgaa caagatgtgg tgttgcactg ccggaacggc ggcagttacg
    14821 acgtgcatta cagtattacg ccgttaagta ctctggacgg cagcaatata ggctcggtac
    14881 tggtgattca ggacgtcacc gaatcacgca aaatgctgcg ccagctgagc tacagcgcct
    14941 cccatgatgc actgacgcat ctcgccaatc gcgccagttt tgagaagcaa ctgcgtatcc
    15001 tgctgcaaac ggtaaacagt acacatcagc gacatgccct ggtgtttatc gatcttgatc
    15061 gctttaaagc ggtgaatgac agcgccgggc atgcggcggg tgacgcttta ctgcgcgaac
    15121 tggcgtcgtt gatgctgagt atgctgcgct ccagcgacgt gctggcgcga ctcggtggtg
    15181 atgaatttgg tctactgttg ccagactgta atatcgaaag tgcgcgtttt atcgctacac
    15241 gcattatcag tgccgtgaat gattatcact ttatatggga aggccgtgtg catcgggtag
    15301 gtgccagtgc cgggattacc ttgattgatg acaacaatca tcaggcggca gaagtgatgt
    15361 cgcaggctga tatcgcctgt tatgcctcca aaaatggtgg acggggccgg gtgacggttt
    15421 acgaaccaca gcaagctgcc gcacatagcg aacgggcggc gatgtcgctt gatgaacagt
    15481 ggcggatgat taaagagaat cagttgatga tgatcgccca cggtgtcgct tcgccgcgga
    15541 tcccggaagc gcgtaatttg tggctgattt cacttaagct ctggagttgc gaaggcgaga
    15601 ttattgatga acaaaaattt cgtcgtagct tcagcgatcc ggcacttagc catgctcttg
    15661 accgacgggt attccacgaa tttttccagc aggccgcaaa agcggttgcc agtaaaggca
    15721 taagcatcgc cctccccctt tccgttgccg gtttgagtag cgccacgctg gtgaatgatc
    15781 tgcttgagca gctgggaaat agccctctac caccacggtt attacatctg attattccgg
    15841 ctgaagcgat tttagatcac gcagaaagcg tgcaaaaact gcggctggtg ggatgtcgga
    15901 tagtgctcag ccaggtgggc cgcgatctgc aaatcttcaa ctcgctgaaa gcgaatatgg
    15961 cagattacct gctacttgat ggtgagttat gcgccaacgt gcagggtaat ttgatggatg
    16021 agatgctgat tacgattatt caggggcacg ctcagcgact cgggatgaaa accatcgccg
    16081 ggccagtcgt tttaccctta gtgatggata cgctttctgg catcggcgtc gatctgattt
    16141 atggtgatgt gattgccgat gcccaaccgc tggatttgct ggtgaatagc agttatttcg
    16201 cgattaactg agagagcgtt tgcccattgc ctgatgcgac gtatcaggcc tacaattcgt
    16261 ccgttccgtc tggttgccag ccttccgtat accagatatg caacagcgca taagaacgcc
    16321 agggcttcca gcgctcggca taacggcgga tttgtgccgg tgtcatcccc ggaaatcgct
    16381 gtttaatcag ataatcatcc ggcagaaaaa catctttcgc ctgccagcca cgcaaagcga
    16441 aataattcgc cgtccagcgc ccgatacccg gaaaagtttg cagcgttttc atcgcctgct
    16501 ccacatcgcc cggaattgtc attggtaagc tgccctccag cgccgcattt gccagatgaa
    16561 tcagtgcctc tgcccgtttc aacggcatgc ctaacgcttt taatgcctgc gggtcggctg
    16621 ctgccagccg ctgaggcgtg gggaagcaga tatactccgg aaaatcatcc agccgttcgc
    16681 cataaagctg tacaactttg gcagtcaatt ttgccgccat cgccacgctc accagttggc
    16741 ctaaaatcgc ccgcacaccc tgctcaaaag catcaataca gccgggtaaa cgcaatccgg
    16801 gccgcgccgc gcctaacttg cccaacgcac cgttaacaat ctgcgggtta cattgcagat
    16861 caaacaggcg gctcattttc gccagacact cagcggcaac gggttctaaa cctgcactta
    16921 aatttatgtg cagagtatgg cgggctatat ccggaatagc agtcaccacg ccgcgatatt
    16981 cgcccaccgc cagactacgg gcataataat cgtcagcgac cgtttccaca ccactcactg
    17041 cacgggcggc gagaaatccc aacatccacg accagtcata cggcggctgc cagttcaggg
    17101 tatacatcgc atctccttat tcatccgctt tcagcataaa cgttattcag acgctacgct
    17161 ttgctttcat attccggttg tcgcgacggc aacatttcgc taaagtcacg ccccttcttc
    17221 accggcatgg ggattatttc gtgtttattg gttttgatta cggtacagca aactgttcag
    17281 tggcggtcat gcgtgacggt aaaccgcact tgctaaaaat ggaaaacgac agcacgctgc
    17341 tgccttcaat gctttgcgcg ccaacgcgtg aagcggtaag cgaatggctg taccgccatc
    17401 atgacgttcc ggcagacgac gatgaaacgc aggcgttgtt acgacgggcg attcgttata
    17461 accgcgaaga agatatcgat gttacagcga aaagcgtgca gttcggtctt tcctcactgg
    17521 cacagtacat tgatgatcca gaagaagtgt ggtttgtgaa atcaccaaaa tcgttcctcg
    17581 gtgccagcgg cttaaaaccg cagcaggtag cgctgtttga ggatctggtc tgcgcaatga
    17641 tgttgcacat tcgccagcag gcgcaggcgc agctgccaga agcgattact caggcggtga
    17701 ttggtcgccc gatcaacttc caggggctgg gcggcgatga agcaaacgcc caggcgcaag
    17761 ggattctgga acgcgcggcg aagcgtgccg gattcaggga cgtggtattc cagtatgagc
    17821 cggtcgcggc tgggctggat tacgaagcca ccttgcagga agaaaaacgg gtgctggtgg
    17881 tggatatcgg tggtggtacg actgactgtt cattgctgct gatgggaccg cagtggcgtt
    17941 cgcgtctcga tcgtgaagcc agcctgctgg gtcacagtgg ttgtcgtatt ggcggtaacg
    18001 atctggatat cgccctggcg tttaaaaacc tgatgccact gctgggtatg ggtggcgaaa
    18061 ccgaaaaagg catcgccctg ccgatcctgc cgtggtggaa tgcggttgcc atcaacgacg
    18121 tacctgcgca gagtgatttc tacagtagtg ccaacggtcg tctgcttaac gatctggtac
    18181 gcgatgcccg cgaaccggaa aaagtggccc tgttacagaa agtctggcgt cagcgtttaa
    18241 gctatcgcct ggtacgcagc gcagaagaga gcaaaatcgc cctttcaaac gctgcggaaa
    18301 cccgcgcctc actgccgttt atcagcgatg aactggctac gctgattagc cagcaagggc
    18361 tggaaagcgc cctcagccag ccactggcgc ggattctgga acaggtgcaa ctggcgctgg
    18421 ataacgccca ggaaaaaccg gacgttatat acctgaccgg cggtagcgcc cgttcgccgc
    18481 tgattaaaaa ggctctggca gaacagttgc cgggcattcc gattgcaggc ggcgatgact
    18541 ttggctccgt caccgccggg ctggcacgct gggcggaagt ggtatttcgt taaaagaaaa
    18601 tggctcagca gcggcttaaa tctgcctgcg cctgagccag tttttctgcc gcctgacgta
    18661 acggttccat caacggcatg gtccgtgctg gagcataaag gcggaatcgc tgcaactctg
    18721 tcgcgccgac agtcaacgcc gattccagcc gatgtcgttt ggcggccatt accgcctgtc
    18781 tgtctgctgg cgttaccgct tcgttcggcc tgaacacaaa acggcggtca cagctcgctt
    18841 tccagtcgat aaccgcctgg gtcagatggt cgccaaaccc tttaacttgc ttaacgccac
    18901 gacgggtaac atccgctgct gtttcaatac caaaagaccg taacgccgct ttacgcgcag
    18961 ggccaacgcc gggaatagag gcaaaaaatc cctccagaaa cttctgcttc tgccgttccc
    19021 ttgcggtgtc ataaagtgct gccagagcgc gtttctcctc ttccggtaac ccgagaattt
    19081 cgtccttcat tttttcgagc atcgcccgtt tggcgataaa accttccagc ccgcccaact
    19141 gttggatctg gctgactaaa tgctcatagt ccattttcgc gcgattaaat gcctcacggc
    19201 gttgctggat ttctgctttg tacactttgc ttgtcagact gccaataatc cagatagccg
    19261 acagcacagg aaccaaaata atataacgcg gttctgcctg aaggccgcaa agcaacagcg
    19321 ataacgctga cagtgcgatc tcaatcagaa tgatgtattc gcgccgtaac aggcctaaag
    19381 gaagcggcct gccagtcggt tggaaatgat cgggtaatgg caattgcaat gctggcggtg
    19441 ctactgacgc catcaccatc gcccagactt tcgccagcac aaaatcaccg ctggtggtaa
    19501 tgacctcttc accgagatca acaaaataga taacgccttg attatccagc gtacaccacg
    19561 ggcagtcggt caaatgagcg ggataaacat gcattgccga aacgggacat ttctttaact
    19621 gttggcgcag agaatcaagt gccgctaccc acgctttagc cgtcggacgc ccggttgcca
    19681 cgccactttc cgtgaatgcc tgctgaaaca tggcttcaac atcactcggt aacatcgata
    19741 gcggaatcga tcgtggcggc ggttttaaac cacgtcgctg attatctgac gcgtaggcat
    19801 aacggaaatg ggtaatatcc gtctccagcg cattacccgc atcagagata agcggcacac
    19861 cagaataagg atgccggcca ccaaacaaga cgtgaaaaat cagcaacgca aggccaaaat
    19921 tatcgtgatt ttctgtgcgt tcaaagccga caaatgacga caaggtttgt agctctggcg
    19981 gcgtaaaatg cgacacgccg acttcgcata aatgcagtgt gccattgacg ttaatctgaa
    20041 aggagtcgct atcaatcagt accactttgc tgtcgcgacc taccataaaa ctgttctggt
    20101 ttacgtcacc cacgacgtgc ccgtgctcgt gaaccgtagc aaaagatgaa gcaatattgc
    20161 gcgcaacata gagtagaaaa tcccacgcac aatgagggta actctggcga cgatgtgccg
    20221 ggctatagat catatgaatc ggttctttac cagaaacttt tggcatcata aaaccgataa
    20281 cttttccgcc tcgtccaccg tgaagcgttg cctgcggcca ggcgacataa ttcaacaact
    20341 gcgcgtcagc tgtcgcagcc ataaaggcaa gtttgtcctg ttttgaggcg ggtggcggcg
    20401 tgtgataaat cttggcgacg ctatcgacaa actcctcgat atcataaacc gcgccttcac
    20461 cgcctttgcc cagttcacgg cctggcgtaa cgcactcacc agtagcagta tataaagtgg
    20521 gtttcatggt aaccacagcg ccagtgccaa cgttttatca tcatccgtac gctcgtttac
    20581 cgccgggctg gacaaaaact gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt
    20641 tactgacgcc agtccattga aaaacggggt aaagaaaggc acatgaggag aattatccag
    20701 catatttaac gccagccgct gaataccatc cgtaaatgct gcaactttat gcgcacgccc
    20761 agtgctggtg aaagtttcca gtctggaaac ggcatcttcg tcggtaatga agtgcgtcat
    20821 gttggcatat tcacccacca tcggcgtgag aggcaattgc agaccgtgac ccaaatctac
    20881 caccacgccg ccatcgccaa tctgcataat caaggtgccg tcaggcgatg aaatcaatcc
    20941 cagaaacgtg caggcaaaat cgcgaactgc taatgctttg gcttctgcct cggcaaaaag
    21001 tcgttgacga atagtcagta ccatatttgt ggcgaggaca tcattaagcc ccaattcacc
    21061 gtcctgtact ttttgcacca taaacgccat cgcttcattg acggcgagcg tcgcgccttc
    21121 tcctccctgc gaaacgctac ctgcgccatc agcaaggaac atcaccagta atggttgctg
    21181 gtcgtttaac cagacaacct gcatctggca ggcatcctga caaggcaaat cagcactaat
    21241 gtgcgatgtt ccaacagcgg atgcataaac cagacgccag ctcacactga cgtccagcct
    21301 tttggcgctt ccagcacaac ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa
    21361 ctggataacc agctaaacag ttcacggaat tgtaatccct gcagtggcaa aggctgacga
    21421 acgctgattt gtgccagagt cttcatatcc gccccctgaa caccgatgga aaagaaggca
    21481 aacttcttat cttcttcccc ctgaaacact ttgttggcgg cagcctgcca ctcatcggtt
    21541 ggagcgccat cggtgatcag gaaaatccac gggcgataat acgaaatacc atttgcgcgg
    21601 tactcacgtt tcctctcttc aaccatatct agggctttgg taatggcggc ccccataggc
    21661 gtatccccct gggcaaacag gatgggcggg aaaaagttag cggcgctggt aaagggttgt
    21721 tccacatgca ccgggccgaa cgtcacaatg ccgagttcca ctctttttaa agccagcgga
    21781 tcagcaagca gttcatcacg aaaggtaacc aatccggcgt taagttcatt aataggtctg
    21841 ccgctcatcg agccagaaac atccagcagc aaaatgcagg ggcaacgtgg ttccgggtta
    21901 ctggcaaaat cgctggtggc aaacgtgatt tgttcgctca taagaaatcc tttcttagag
    21961 aattatcgag gtccggtatc ataccgattg caataatgaa ataagagcaa acgaagaaac
    22021 tttcagaatt aggcccgtcg gcgcagaaaa ttttgctatt cgcctcgcaa gaacggagag
    22081 ccgtcgattg acactaaggg cggagtgaca taatttcagg agtgagggtt agggagaggt
    22141 ttccccctcc ccctggtgtt cttagtaagc ctggaagcta atcactaaga gtatcaccag
    22201 tatgatgacg tgcttcatca taaccctttc cttattaaaa gccctcttct ccgggagagg
    22261 ctttcccgtt tcagcgtccc gctgaaatca tcggcttacc tcctttcgcc ctgaatgcag
    22321 tctatcgcta acgcgtgaat aaaatagttt cctctgtgat tattgcgtaa cgactcgcaa
    22381 agaatatttg ctggcaggat cgcagactac aaagcctacg gattgacaat cttatcgtga
    22441 aggcatactt tcaggagtga gggtagagcg gggtttcccc cgccctggta gtcttagtaa
    22501 gcggggaagc ttatgactaa gagcaccacg atgatgagta gcttcatcat gaccctttcc
    22561 ttatttaagg ccccttcctc gggaggggct ttcccgtttc agcgtcccgc tgaaatcgtc
    22621 ggcttacctc ctttcgccct gtatgcagtc tatcgctgac ttgcggataa aatagtttcc
    22681 agtgtgatta ttgcgtaacg tctcgcaaag aatatttgct ggcaggatcg cagactgcaa
    22741 agcctgcaga ttgacaatct tatcgtgaag gcatactttc aggagtgagg gtagagcggg
    22801 gtttcccccg ccctggtagt cttagtaagc tggccagcta atgactaaga gcaccacgat
    22861 gatgagtagc ttcatcatga ccctttcctt atttatggcc ccttcctcgg gaggggcttt
    22921 cccgtttcag cgtcccgctg aaatcggcgg cttacctcct ttcgccatgc aagcagtcta
    22981 tccctaacgc gtagataaaa tagtttcctg tgttattact ggatgcgtgc tcgcaaatgt
    23041 gcccgtcatt cagacaattc ccgacagtgt ttcataattc ctccattttt ctcccttatt
    23101 ggctggctac actagtatca ttccgcgaaa cgtttcagga agagaaactc ttaacgatga
    23161 aaggcagtta taaatcccgt tgggtaatcg taatcgtggt ggttatcgcc gccatcgccg
    23221 cattctggtt ctggcaaggc cgcaatgact cccggagtgc agccccaggg gcgacgaaac
    23281 aagcgcagca atcgccagcg ggtggtcgcc gtggtatgcg ttccggccca ttagccccgg
    23341 ttcaggcggc gaccgccgta gaacaggcag ttccgcgtta cctcaccggg cttggcacca
    23401 ttatcgccgc taatactgtt acggtgcgca gccgcgtgga cggtcaactg atggcgttac
    23461 atttccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    23521 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    23581 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    23641 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    23701 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    23761 tcgatggtcg cgttggtctc aagcaggttg atgttggtaa ccaaatctcc agtggtgata
    23821 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    23881 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg gtggtagaag
    23941 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    24001 aaatcgatgc cacaaccggt atgattaaag tgaaagcacg ctttaataat cagaatgatg
    24061 tgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    24121 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    24181 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    24241 tggtgatccg tgcaggtatt tctgcgggca atcgcgtggt gacagacggc attgatcgcc
    24301 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    24361 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    24421 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    24481 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    24541 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    24601 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    24661 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    24721 aacattaccg ctcgatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    24781 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    24841 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    24901 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    24961 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    25021 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    25081 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    25141 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    25201 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    25261 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    25321 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    25381 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    25441 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    25501 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    25561 ggtgtttctc gatttttcaa tcaataacct gacactgatg gcgttaacta tcgccaccgg
    25621 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    25681 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    25741 gctgatcttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    25801 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    25861 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    25921 acagaaccgc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    25981 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    26041 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    26101 caatggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    26161 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    26221 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    26281 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    26341 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg atctgactat
    26401 tgatactcag gtcagccgca cccagtacca gtttaccttg caggccacct cactggatgc
    26461 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    26521 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    26581 cgccagccgt ctggggatca gcatggcgga tgtcgataat gccctgtaca gcgcgtttgg
    26641 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtag tgctggagca
    26701 caacaccgaa attaccccgg gcctcgcgac gctggatacc attcgcctga ccagtagcga
    26761 cggcggcgtg gtgccgttaa gctcaattgc caaagttgaa cagcgttttg cgccgctttc
    26821 catcaaccat ctggatcagt tcccggtaac gaccatctcc tttaacgtgc cggataacta
    26881 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    26941 ggatatcacc acgcaattcc agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    27001 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    27061 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    27121 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    27181 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    27241 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    27301 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    27361 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    27421 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    27481 attgtggacc aaaagtcgct ttgctcgcca tgaagaggaa gcgtaagtga agttttttgc
    27541 cctcttcatt taccgtccgg tggcgacgat tttactgtcg gttgccatta ccctgtgcgg
    27601 catactgggc ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    27661 tatggtcagc gcctcgctgc ccggtgcgtc accagaaaca atggcgtctt ccgttgccac
    27721 gccgctggag cgctcacttg ggcgcattgc cggagtcagt gaaatgacct ccagcagttc
    27781 gctcggcagc acgcgtatta ttttgcagtt tgattttgac cgggatatca acggcgcggc
    27841 gcgcgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    27901 ccgtccgacc tatcgcaaag ctaacccgtc ggatgcgcca attatgatcc tcacgctgac
    27961 atccgatact tattcgcagg gtaaactgta cgatttcgcc tcgacgcagc tggctccgac
    28021 gatttcgcaa atcgacggtg ttggtgatgt cgatgtcggc ggcagctcac tgcccgccgt
    28081 acgcgtcggg ctgaatccgc aggcgctgtt taatcagggc gtctcgctgg acgacgtacg
    28141 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    28201 ccgctggcag atccagacca atgatgagct aaaaaccgcc gccgaatatc agccgctgat
    28261 tattcattac aacaacggcg gcgcggttcg tctgggcgat gtggcgacag tgaccgactc
    28321 tgtacaggat gtacgtaacg ccgggatgac caatgccaaa ccggctattt tgctgatgat
    28381 ccgcaagctg ccggaagcca atattatcca gacggttgac agcatccggg caaaattacc
    28441 ggagttgcag gaaaccattc cggcggcgat tgatctgcaa attgctcagg atcgcgcccc
    28501 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    28561 gattctggtg gtgtttttat tcctgcgctc gggtcgcgca actattattc ccgccgttgc
    28621 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggtt tcagtctcaa
    28681 taatctttcg ttaatggcgc tcaccatcgc tactggtttc gtggtggatg acgccatcgt
    28741 ggtgctggaa aacattgccc gtcatctgga agcgggaatg aaaccgttgc aagccgcact
    28801 gcaaggtact cgcgaagtcg gttttacggt gctgtcgatg agtctgtcac tggtggcggt
    28861 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg aatttgccgt
    28921 gacgctttct gtcgccattg gtatatcgtt gctggtttct ctgacattaa cgccaatgat
    28981 gtgtggctgg atgctgaaag ccagcaagcc gcgcgagcaa aagcgactgc gtggttttgg
    29041 tcgcatgttg gtagccctgc aacaaggcta cggcaagtca ctgaaatggg tgctcaatca
    29101 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct ggctgtatat
    29161 ctcgatcccg aaaaccttct tcccggagca ggacactggc gtgttgatgg gcgggattca
    29221 ggcggatcag agtatttcgt ttcaggcgat gcgcggtaag ttgcaggatt tcatgaaaat
    29281 tatccgtgac gatccggcag tggataatgt caccggcttt acaggcggtt cgcgagtgaa
    29341 cagcgggatg atgtttatca ccctcaagcc acgcgacgaa cgcagcgaaa cggcgcagca
    29401 aattatcgac cgtctgcgcg taaaactggc gaaagaaccg ggggcgaatc tgttcctgat
    29461 ggcggtacag gatattcgcg ttggtgggcg tcagtcgaac gccagctacc agtacacgtt
    29521 gttatccgac gacctggcgg cactgcgaga atgggagccg aaaatccgca aaaaactggc
    29581 gacgttgccg gaactggcgg acgtgaactc cgatcagcag gataacggcg cggagatgaa
    29641 tctggtttac gaccgcgaca ccatggcacg gctgggaatc gacgtacaag ccgccaacag
    29701 tctgttaaat aacgccttcg gtcagcggca aatctcgacc atttaccagc cgatgaacca
    29761 gtataaagtg gtgatggaag tggatccgcg ctatacccag gacatcagtg cgctggaaaa
    29821 aatgttcgtt atcaataacg aaggcaaagc gatcccgctg tcgtatttcg ctaaatggca
    29881 accggcgaat gccccactat cggtgaatca tcagggatta tcggcggcct cgaccatttc
    29941 gtttaacctg ccgaccggaa aatcgctctc ggacgccagt gcggcgatcg atcgcgcaat
    30001 gacccagctt ggggtgcctt cgacggtgcg cggcagtttt gccggcacgg cgcaggtgtt
    30061 ccaggagacg atgaactcgc aggtgatcct gattatcgcc gccatcgcca cggtgtatat
    30121 cgtgctgggt atcctttacg agagttacgt acatccgctg acgattctct ccaccctgcc
    30181 ctcggcgggc gttggagcgc tgttggcgct ggagctgttc aatgccccgt tcagcctaat
    30241 cgccctgata gggatcatgc tattaatcgg catcgtgaag aaaaacgcca ttatgatggt
    30301 cgattttgcg cttgaagccc aacggcacgg taacctgacg ccgcaggaag ctattttcca
    30361 ggcctgtctg ctgcgttttc gcccgattat gatgaccacc ctggcggcgc tgtttggtgc
    30421 gctgccgctg gtattgtcgg gcggcgacgg ctcggagctg cggcaacccc tggggatcac
    30481 cattgtcggc ggactggtaa tgagccagct ccttacgctg tataccacgc cggtggtgta
    30541 tctctttttc gaccgtctgc ggctgcgttt ttcgcgtaaa cctaaacaaa cggtaaccga
    30601 gtaaatgaca gatcttcccg acagcacccg ttggcaattg tggattgtgg ctttcggctt
    30661 ctttatgcag tcgctggaca ccaccatcgt aaacaccgcc cttccctcaa tggcgcaaag
    30721 cctcggggaa agtccgttgc atatgcacat ggtcattgtc tcttatgtgc tgaccgtggc
    30781 ggtgatgctg cccgccagcg gctggctggc ggacaaagtc ggcgtgcgca atattttctt
    30841 taccgccatc gtgctgttta ctctcggttc actgttttgc gcgctttccg gcacgctgaa
    30901 cgaactgttg ctggcacgcg cgttacaggg cgttggcggc gcgatgatgg tgccggtcgg
    30961 cagattgacg gtgatgaaaa tcgtaccgcg cgagcaatat atggctgcga tgacctttgt
    31021 cacgttaccc ggtcaggtcg gtccgctgct cggtccggcg ctcggcggtc tgctggtgga
    31081 gtacgcgtcg tggcactgga tctttttgat caacattccg gtggggatta tcggtgcgat
    31141 cgccacattg ctgttaatgc cgaactacac catgcagacg tggcgctttg atctctccgg
    31201 atttttattg ctggcggttg gcatggcggt attaacccct ggcgctggac ggcagtaaag
    31261 gtacaggttt atcgccgctg gcgattgctg gcctggtcgc agttggcgtg gtggcactgg
    31321 tgctttatct gctgcacgcc agaaataaca accgtgccct gttcagtctg aaactgttcc
    31381 gtactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt ggcagtggca
    31441 tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg ccgtttcatg
    31501 ccggactgat gatgatcccg atggtgcttg gcagcatggg aatgaagcga attgtggtac
    31561 aggtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg ggtctgtcgc
    31621 tggtcaccct gttgtttatg actaccgccc tgctgggctg gtactacgtt ttgccgttcg
    31681 tcctgttttt acaagggatg gtcaactcga cgcgtttctc ctccatgaac accctgacgc
    31741 tgaaagatct cccggacaat ctggcgagca gcggcaacag cctgctgtcg atgattatgc
    31801 aattgtcgat gagtatcggc gtcactatcg ccgggctgtt gctgggactt tttggttcac
    31861 agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc tggcttagca
    31921 tggcgttgat catcgccctt ccggcgttca tctttgccag agtgccgaac gatacgcatc
    31981 aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc gacccggtat
    32041 taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga tcagtatgca
    32101 ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc atggtaatga
    32161 acagcgatta caactgttaa gtgatgcgct tggcgagcag tatgcgcagc atggcaactg
    32221 gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat ttgaacacga
    32281 taattcggaa gataaacccg gcccgggtat gccaccgcac ggctggcgta cccagttctg
    32341 ggtggttgat caaaacaaca aagtgctggt tggtccgcga gcgccgattc cacctgacgg
    32401 tacacggcga cccattctgg tcaacggtgc ggaagttggc gcggtgatcg cctcccccgt
    32461 tgagcggtta acgcgcaata ctgatatcaa tttcgataaa caacagcggc aaaccagctg
    32521 gttgattgtc gccctggcaa cgttactcgc ggcacttgcc acttttctgc tggcgcgcgg
    32581 tttactggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg cgggcgattt
    32641 cactacccgc gtaacgccca ccagtgaaga tgaactgggc aaactggcgc aagacttcaa
    32701 ccagcttgcc agcacactgg agaaaaacca gcaaatgcgg cgcgatttta tggccgatat
    32761 ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag ccattcagga
    32821 tggcgtgcgt aaattcacgc cggagacggt ggcgtcttta caggcggagg tcggtacact
    32881 gaccaaactg gttgacgatc tccatcagtt gtcgatgtct gatgaaggcg ctctcgccta
    32941 tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg catttcgcga
    33001 acgattcgcc agtcgtggcc tgaaactgca attttccctg ccagacagta ttaccgtatt
    33061 tggcgatcgc gaccgtttaa tgcagttatt caataattta cttgaaaaca gcctgcgcta
    33121 tactgacagc ggcggcagcc tgaaaatctc tgccgagcag cacgacaaaa cggtgcgcct
    33181 gacctttgcc gacagtgcgc caggtgtcag tgacgagcag ctacaaaaat tatttgaacg
    33241 tttttatcgc accgaaggtt cccgcaaccg cgccagcggt ggttccgggc tggggctggc
    33301 gatttgcctg aacattgttg aagcacataa tggccgcatt attgctgccc attcgccttt
    33361 tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga gagaagtatg
    33421 accgagttac caatcgacga aaacacacca cgtattttga tcgtggaaga tgaaccgaag
    33481 ctggggcagt tgctcattga ttatctgcgt gctgcgagct atgcgccgac gcttatcagc
    33541 cacggcgatc aggtactgcc gtatgtgcgc cagacaccac cggatctgat cctgttggat
    33601 ctgatgctcc ctggcaccga tggcttgacg ctgtgccggg aaattcgtcg tttttctgac
    33661 attcctatcg tgatggtgac ggcaaaaatc gaagagatcg accgcctgct cggactggag
    33721 attggcgcag atgactacat ctgcaagccg tacagcccac gggaagtggt agcgcgcgtc
    33781 aaaaccattt tgcgccgttg caaaccgcag cgcgaattgc agcaacagga tgccgaaagc
    33841 ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcggtaaaat gcttgacctg
    33901 acacctgcgg aatttcgtct gttgaaaacg ctctctcatg aaccaggaaa agtgttctcc
    33961 cgcgagcaat tgctcaatca tctttatgac gactaccgcg tagtaaccga ccgcaccatt
    34021 gacagccaca ttaaaaacct gcgccgcaag ctggaatctc tcgacgccga acagtcattt
    34081 atccgcgccg tttacggcgt tggttaccgc tgggaagccg acgcctgccg catcgtttag
    34141 ttttagcgac atgactttgt tagccggaga tggcgttcag accagatccg gcaacattat
    34201 cccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc agaaaatcgc
    34261 tcatacttta atcggtaaac agcaccttta gatgctgttt tcgatacgca atttcaatca
    34321 aggagtcatt atggctggtt ggtttgaact cagtaagagc agtgataatc agttccggtt
    34381 tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctgtata catcaaaagc
    34441 ctctgcggaa aagggcatcg cgtcagtgcg tagcaacagc ccgcaagaag aacgctatga
    34501 gaaaaaaacg gcaagtaacg gcaaattcta tttcaatctg aaagccgcca atcatcaaat
    34561 tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt
    34621 taaagccaat ggcacaagcc agacggtgaa agacaatacg taattacgat gccgggcgtg
    34681 ggttgttggc gtccggcttt attttctctc atcacagagt tactatccct caagaaatta
    34741 ttaaccatga aatgagtttt ctcctcataa ctgataagga aaattatgct caaaggattg
    34801 cgtacattat tttcacgcag ggttttgttt aacgtcgaaa tgaataattc tgttgactgt
    34861 gaattacttc tcatccagca acaactccag gcattgatag ccgaaccagg caattattta
    34921 gcacatgatt gtgatctcaa cgatactctg gttctttgcc agcttaccca tcatcacaat
    34981 ggatatatac gagaaaaggc cgtgcagtat ctggggcaca aagaggatgt tgcggctata
    35041 gaagaattat tacttgcagc taatgactgg gtaaaagagg ttcgccttgc tgcaaccata
    35101 gcattacgtc atttaatgaa ggataagaat gcagcggcat ttgccgttaa tctacccatg
    35161 atacgtaacc ttctccaatg ccagcgttac gatcaccgtg catttgtaga cgagatactt
    35221 agatatttat tgacagaaaa taaccgccca caattaacgt attggcttac ttgccctgat
    35281 aaaaagctct cccgcgcggt attacaaacg ctgattgaat acggttactt taatgatgaa
    35341 tcttcactgc tttcaatact cgataaaccc gatgagggtt tgcgaatgct ggcgaccacc
    35401 cactggctgc gtcaacaatt accattaagc gaagcggtat taactcgtct gttaaaagac
    35461 cgctggccgc gtattcgaca agcaacgtta tttagcctca ctgaccgagc aatagaaatg
    35521 cctccggcgt tgcacagcac attactgctg gataacaata tgttgatccg cctacgcgct
    35581 aaaaatatgc ttcacgaagt catggatgtt ccgcaattct ggcgtcatgt tgtcacttct
    35641 gcggaatata ctccctcaca gcgacgggcc gctttatacg ggctggattc cattcatgat
    35701 cccaatattt taaaactggc agaatgggga ttatcacaga atgtattccc actgcgttta
    35761 gccgctatgc acattcttgc aaaagctaat cctcgttgtg gcgtcaaaga gactattctg
    35821 accacgctgg ctaacccgga cgccgccggg ttgcgattta tggttaacat ctgtgtatgg
    35881 tgtcgcgttc cgctcacgtt cgaggaaata cgtcagttgc aggaaaacgc gccgtcagtt
    35941 aaacatgctt gcgcatattg tcggctgtat cataacttga acaaatggga cggtttaatt
    36001 cttcttcttc aatcgcaaca caagctaact gaagaatttg caggtaaaca gctcgcgata
    36061 tggcaacgca atttcaatct ttcgggcatt caaccgaatg ctcttcagcg tcagcaactt
    36121 caggcgcttt ttacccgcaa tccggaactt cacaacagat tatggggtta cattcctttt
    36181 aaataacttc gaattataga cttacgatga caaatgtacc tgctgacgag cggaaccaaa
    36241 acgaaacaat acgtgaagct ttacgcttgc tacgagaaaa acttgccgag ggactacgca
    36301 gtggcgaagc ccaaccgtgg gaaaaggatc tgtttttaca taaagtgaag gccggtaaca
    36361 aataaatccc ccagtctttt ccctcacatt tgcactggag ccaaccccgc cgattgcgct
    36421 acaatgcccg cccttaaagt gggggcactc ccctaaccgc ttcatcaggt gaagcggatc
    36481 tgacctgtca tcagaacgag agaattatgt ttaaaccgga actcctttcc ccggcgggaa
    36541 cgctgaaaaa tatgcgttac gctttcgctt atggcgcaga tgctgtttat gcgggccagc
    36601 cgcgttactc cctgcgtgtg cgcaacaacg aattcaacca cgaaaatctt cagctcggca
    36661 tcaatgaagc ccacgcgctg gggaaaaagt tttatgtcgt ggtcaacatt gcaccgcaca
    36721 acgccaagct gaaaaccttt atccgtgacc tgaaaccggt ggtggaaatg gggccggatg
    36781 cgctgattat gtccgatcca gggctgatta tgctggtgcg tgagtacttc cctgaaatgc
    36841 cgatccacct ctcggtgcag gctaacgccg taaactgggc gacggtgaaa ttctggcagc
    36901 aaatgggcct gacccgcgtg atcctctctc gcgaactgtc gctggaagag attgaagaga
    36961 tccgcaatca ggtgccggat atggagatcg agatcttcgt tcacggtgcg ctgtgcatgg
    37021 cctactccgg tcgctgcctg ctctctggct atatcaacaa gcgcgatccg aaccagggta
    37081 cctgcaccaa cgcctgccgc tgggagtaca acgtccagga agggaaagaa gatgacgttg
    37141 gcaacatcgt acacaagtac gagccgatcc cggtgcaaaa tgttgagccg acgctaggta
    37201 tcggcgcgcc aaccgacaaa gtgtttatga tcgaagaagc ccagcgtccg ggcgagtata
    37261 tgaccgcgtt tgaagatgag cacggcactt acatcatgaa ctcgaaagat ctgcgcgcca
    37321 tcgcccatgt agaacgcctg accaaaatgg gcgtgcattc gctgaaaatc gaaggccgta
    37381 ctaaatcttt ctactattgc gcacgcaccg cacaggttta tcgtaaagct atcgatgatg
    37441 ccgctgcggg caaaccgttc gataccagcc tgctggaaac tctggaaggt ctggcgcatc
    37501 gtggctatac cgaaggtttc ctgcgtcgtc atactcacga cgattatcag aactacgaat
    37561 acggttattc agtttctgac cgccagcagt ttgttggtga gtttaccggt gagcgcaagg
    37621 gggacctcgc agcggtagcg gtgaaaaata aattctccgt tggcgacagc cttgagctga
    37681 tgacgccgca aggcaacatt aactttaccc ttgagcacat ggaaaacgcc aaaggtgtag
    37741 cgatgccggt cgcaccaggc gatggttata ctgtgtggct cccggtcccg caggatcttg
    37801 aactaaatta cgcgctgctg atgcgtaatt tctccgggga aaccacgcgt aacccacacg
    37861 gtaagtgatt aatttcgatt atttttcccg gatggaaaat tcttagaaac cgatcacata
    37921 cagctgcatt tattaaggtt atcatccgtt tcgctgaaaa acataaccca taaaatgcta
    37981 gctgtaccag gaaccacctc cttagcctgt gtaatctccc ttacacgggc ttatttttta
    38041 tgcataagcc ctatccctgg tcaccgtctt ccattgacca catcgataga atctcccttc
    38101 atagcacgat gcctttcacg taacggcatc gtgctcgcac aggttccggc taagcacaac
    38161 cagaacgcgc atgtttgatg cttaccaaaa aatattctca ctctccacat ttgaatgtca
    38221 gacgagcgac accatgtaat cctacacctt ctgtcttcag ctcaactatt tgcatttttt
    38281 tgccctgagt aacacagaaa tgagttgcat cattttttac tatattttct gcaccagata
    38341 ttctactcct ggctaaagaa gcttcggctt cggtgtagta ttggttatcg agtttacgct
    38401 gaatattact tttatatgca agaccaaatt taccgatact tgtctcatca ttatgcacag
    38461 cacaaccaga cataataaaa atactaatta atgatatagc agctatcttt ttcatctcac
    38521 ctttccccat taaataccaa cgacactctc tagtgtttaa atataataat ggctgatcat
    38581 tatatttgaa taggattata ataaatgttc tgtacaacat ttcctacata agtaggactt
    38641 acagacatga aggccctttc caggtaactc catggggctt taatatattt ggaatagtat
    38701 atttaagatg ccactgtttg gttgaatatt aggcatatgc tcttttttga aatttatcgg
    38761 tggctaccgt taatattcgc tgttcccatt gcaagctcct ggtggtaacc actgaatcct
    38821 ccatacttga actgactttt tatcctccga ctttcatcct gttctgactc caccttttct
    38881 tttctgctct acactatcta cagaccaatc ataaaggcac atacgatcat ggcagaattt
    38941 cccgccagct tactgattct taatggcaaa agtactgaca atctaccctt gcgcgaagca
    39001 attatgctgt tgcgtgagga aggaatgacg atccatgtgc gggtcacctg ggagaaaggt
    39061 gatgccgcac gatttgtaga ggaggcccgg aagttgggcg tcgcaacggt aattgccggt
    39121 ggtggcgatg gcaccattaa tgaagtttct acggcgttga ttcagtgtga gggggatggc
    39181 atacccgcgc tgggaatttt gccattagga accgccaatg attttgccac cagtgtaggg
    39241 attcctgagg cactggataa ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata
    39301 gatatggcgc aggtcaacaa acaaacctgt tttattaata tggcgacagg cggatttggg
    39361 acgcgtatta ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac
    39421 atcattcatg gcttaatgcg catggatact ctgcaaccgg accgttgtga aatccgcggt
    39481 gaaaactttc actggcaagg tgacgccctg gtattggtat tggtaacggg cgtcaggccg
    39541 gtggcggtca gcaattgtgt ccgaacgcgt taattaacga tggcttgctg caactgcgca
    39601 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
    39661 acccgagtat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
    39721 cttttaatct tgatggcgaa ccgttgagcg gacaaaactt tcatattgaa atacttccgg
    39781 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
    39841 tcttgaacca gggagaatcc gaaatgccgg atgcggtgta aataccttat ccggcataca
    39901 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccag tttatccacc
    39961 tctgatacag catcatcggt aataataatg tcaatttttt cgattggcag tacctgatta
    40021 aaaccgcgac ggttaaattt cgatgaatcg agaacggcaa tcactttatt agcggctgtg
    40081 accatcgcac cgctaacgga ataaccctca ttaaaggtcg taataccatt aaccgcatca
    40141 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaggga acgttcagca
    40201 atagatccat gcatagatcg cgttttatgg cggaccgtgc caccgcagac gaccagagta
    40261 atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata
    40321 tttttagcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct gtcgagaata
    40381 acggtcatcc cttcatggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg
    40441 ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattaccg
    40501 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
    40561 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
    40621 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
    40681 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
    40741 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca tggaatgagc
    40801 aacactttgc ccggcatggc attacgagcg atgtcacgca ccgcctgggt gaagctttca
    40861 aagcttccac ggtgagcgat taatggctcc aggcttaact tacgttctgt cagcaaccgg
    40921 ctcgccgttt cccactcctg ccccggccaa gggctggaat agttcatcca actgccgata
    40981 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
    41041 tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac cgccagttcg
    41101 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaaacgcag ttcgcgtaaa
    41161 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgaaccgaaa
    41221 gattttgcca gtgccagttt ttctgagcta atgtcgatag ccgtcacact ctttgctccc
    41281 agcgcgacag cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg
    41341 tttttattct cacaaccttg cgctaaatga aaagcatgca gaccaatggt aatcggctca
    41401 ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta
    41461 acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc atattttgcg
    41521 cactgggaat aaaacccttt cagacactct ggacaagtaa aacagggtaa taacggcaca
    41581 caggcaaccg catcgccagg gtgtaaatca tcaacaccgg atcccaccgc atcaatatag
    41641 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
    41701 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt
    41761 ttaatttcag gaatgacgct ttctgcaacg cgcacgatac catcagtatc attcaccact
    41821 gatttcataa aaactcctga ttgttaaggg ggataaactc cccctcagtt aaaaattatt
    41881 ctgcgagaac ggctttctct tgtttaataa aaccacgcgc tctacgccag gtaatgaaaa
    41941 taccggtcag ataaattgcg ccgataataa tgaaaccggg aatattttgc ggggagaaaa
    42001 cctgaatcag taaccaggta atgggagaac cgccctgatc cattgaagcc accatacccc
    42061 cggcttttaa cgccccagca ttagccgcca gttgggtgtg caaaccaata gtttgcgtcg
    42121 cgatccacag agtgatgctc atgatgatga cacccgagat taaggtgcgg aacagatttc
    42181 cacgatgcac ggcgactgcc atcgccacaa agaagccgat ggtggcaaga tcgccaaacg
    42241 gcagtacctg attacccgga acacaaacag caattaaaat ggtgagcggg ataaaaatca
    42301 ggcttaccga taccaccgcc gtatgcccca gcagtaatgc gggatccaag ccaatcagga
    42361 actcctgacc gccgaacttc gcctgtaaac gactacgggc ctgcttagcg atgggcgtta
    42421 aaccatccat gatgggttta atcacccgtg gcattagcag catcactgcc gccgttttta
    42481 ccgccagctg caatacacct ttgacatcgt aacccgcgag gatgccgata atcagcccca
    42541 tcacaaaacc cacggtgaca ggttcgccaa aagggccaaa tttgcgctga atatcatcgg
    42601 cgctaaattt aattcggtta acgcctggga ttttctcgat gatagcatcg accagcaccg
    42661 caatcggccc catatacgcc gacgtaccgt gcggaatagc aataccttcc agctcaaaga
    42721 aatttcgggt atcgcgggca aaccagtcgc cgagcttata aacaaacgcc gcgtgaatta
    42781 ccacacccgc catccctatc atccatgaac cggttgccag atgcagcaac gcgccggtga
    42841 aagtcatatg ccagatattc cagatatcaa tatttaccac ccgcgtcata cgggtcagta
    42901 acatcgccac gttaaccaga atagcaatcg gaatcgccac cagcgcaatt tgcgacgccc
    42961 aggtcattgg tgaagagccc ggccagccga catcgaccac atgcagattc aggtcgaaat
    43021 tttccgccat cgctttcgcc gccggaccaa tggaatccag cattaagcca atcacaaggc
    43081 caatgccaac aaagccaatc cctatatgaa gacccgcttt aaagcaatcg cctgccttca
    43141 tgcctaatat tttagaaaaa ataatgatga caatcggcag catcaccgta gggccgaggt
    43201 cgagaatata acgcatgact tctgaaaaca taggtcaccc ctgtaagata gtcagaattt
    43261 tattttgtaa tgcttcgata ccgacaccag aaacaaaagg catgccgtga actaacggaa
    43321 tatcgccaaa actacgatcc accctggcag tagtgcatat caaatgcacg ccatccatat
    43381 aggtttctat ttcattaacc cgacactgga ttaattcaac aggaatatta tgattctgac
    43441 acaattcttt aatttcttcc gccgccatcg tagaggtcgc aaccgcgcct ccgcaagcga
    43501 caataatctt gcgtttcata aatagtcctt tttattgtta cagagggatt tattcgtctg
    43561 aatctaaaac atactttgtg aagtattcct ttaactgggt ttcaggaaga gtgattagtg
    43621 tctcgacgat atcgggctgt tgtaacttgc caaataaaca gcgtaaaagt ttcaattgct
    43681 gctgcggatt ttccacaatc aacgcaataa ccaacgatac ctccacgtcg ttatcatcat
    43741 ccgcttgctg aaaatgaact ttatttgttg gccttaacag ataaatggct gacgacttag
    43801 catgaatcgc ctcacaatgc ggtattgcaa tggcgtgctg ctcaagcatt atcccggtag
    43861 ggaattctgc ttctctggca attaatgcct gtggccaggt atcatgaacc acacctttgg
    43921 cgagcatctc attaccgata tgggttaaaa cttcgctacg atcgacaaaa gaaattccgc
    43981 tacgaacaaa caggttagtc atataccgtc cttattccgc acagccgtag cgataggcgc
    44041 gcaaaacatc ataaatttta tccataatca gctgatgcgg tattgctgat aattccccgg
    44101 actgaatgcg ttcaaattgt ttgggaagat actgactaat catgcccagt gggatgtcca
    44161 cgccttcaag attcaccatc atcgtttcga cgctattttt aatccgacta tgcggccagt
    44221 aataacgaat acgatccgac aggctgtaac gaatatccag taatgaatcg ttaaaacccg
    44281 tacgataata ttttttccag tattgcggtt cgtcgagcat cacttcttca attaccgcca
    44341 ggcaaccgct gcgattttca ggggcgataa gttcctgctc aatttgtgcc agtgcaaata
    44401 tcgcctcgcg taaagcaaag gttaatgcgg gaccgacttt caatattgca aagtgatcgc
    44461 ggactaattc ccaataagcc gtccgggtct ggtaatcggt agaatgtgct tcataaacca
    44521 ttcgggtgtt ttctatccat tgcgccagcg gctgcgcttc ctgcggctga taatggataa
    44581 tattgctgtg atcaaattcc acgcccggct gcaccacgat ggcaatcacg cgtgttaacg
    44641 cctctgtcag cccacggaca ataaaggcct tttgatgcgt acgtaaagta ttggcggcat
    44701 cttcaacatg ggtgatgtgt actgactgaa tggcgctggc ctcaccgccc ggaaccggaa
    44761 cttcggtgcc aatgacatag ctcagttgct cacgctggca atctgtcgcc acactttccg
    44821 cagcaaagca aagcacagca gctcgttccg caaccgtttc tggtgctaac ggtatgggat
    44881 cccccgcgca ggacattgac gcatcaagat gaattttact gaagccggca cgaacatatt
    44941 cctttaccag ctcgacggat ttttccatcg ccgcatccgc attttcttgc tgccagcagt
    45001 ttggccccag atgatcgccg ccgagaataa tgcgttcgcg tgcaaaccca actttatcgg
    45061 caatcgtaaa aacaaattcg cgaaagtctg ccggtgtcat tccggtataa ccgccaaatt
    45121 gattgacctg gtttgacgtt gcttcaatca gcactttgcg cgtgctgttg cgatcaaatg
    45181 ccagcgccgc ttcgataacc aacggatggg cagaacagac tgaacatatg ccgatatgtt
    45241 caccagcttt atgccgggca attaacgttt tcatgttttt tccttattaa atggcaggtg
    45301 cgttatgccc tgccctcgca gccacaatcg gcaatcactt tgctcaccac atcgcgcatt
    45361 gcggatttag ccgactgcaa ataatcccgg ggctcggtcg cttcagggtg ctcggtcagg
    45421 taatttttta acgcctgcga gaaggcattt ttcagctccg ttgcaacgtt gattttgcat
    45481 atccccagtt tgatggtttg ctgaatatcc ttagtcgata accctgacgc gccatgcagc
    45541 accagcggta agttcaccca ctggcgaatg ttctccagtc tagaaaaatc aagcgccggt
    45601 gcgctggcat acatcccatg agccgtgccg atggcgaccg ccagggaatc aattccggtt
    45661 gcctcggcaa attcacgcgc ctgagcgggg ttggtataaa aagcatcggc ttcattgact
    45721 tgcacatcat cttcctggcc gccaagttgc cccagctccg cttcgacgct gacatcaaag
    45781 cgatggcaaa aatccaccac ctctttgacc cgtgaaatat tttgcgcaaa aggcaaatgc
    45841 gaggcgtcaa tcatgactga gcgcacgcca gaacgaacct tctgagcgat atcgtcaaat
    45901 ttcgtgtgat ggtcgagatg aattgccagt ggatggtgat attgcttcgc catcgcgctg
    45961 accagcgcca acagattttc tgtaccagca tgagtaaatg tgccaggcgt tccggcgatg
    46021 atgaccggcg catgcaggtt ggcagcggtt tccaccacca cttgcatcgt ttcgagattg
    46081 tgaatattga atgccggaac cgcataaccg ccgcgctgtg cgttgttcag catctgcttt
    46141 gtcgatacca cgtgcatttt catatcctgt cgtttgtttt cgttttcaaa atataatgaa
    46201 attatttgtt tttaaatatc gagataacga tcacaaaaac gacaatatga aaattattcg
    46261 aggagtgaaa ggcaaaaaaa cggcctcccg atagggaagc cgtagcaaag tgcgcgtgtt
    46321 tttatgccgg atgcggtgta aacatacgtg gcagtgctat gtaggcatga taagacgctt
    46381 ccgcgtcgca tcaggcatag gttgccggat gcagtgtaaa cgccttatcc gtcctacggg
    46441 gtggtgttgt tcgtataagg cgtatcaggc aatagtaatt ttgctatcga gataaacgtc
    46501 ctgcacggcg ttaatcagtt tcacgccgtc agccatcgat ttcttgaacg ccttacgtcc
    46561 aagaatcagc cccattccgc cagcgcgttt gttgataacc gcagtacgca ctgcatcact
    46621 gaggtcagtt tcaccgcctg cagcaccgcc ggagtttatc aacccagccc ggcccatata
    46681 gcagttagct aactgataac gcaccagatc aatcgggttt tcgctggtta acttgctgta
    46741 cacgcgatcg tcggtataac cgtaattaat cgctttatag ccgccgttat tttccgccat
    46801 tttttgtttg acgatatctg cgccgatggt tgccgccaga tggtttgcct gaccggtcag
    46861 gtcggcggaa acatggtaat caacgccatc tttcttaaag gcggagttac gcaaataggc
    46921 ccacagcacg gtcaccatgc ccagctcatg cgcacgttca aaagccgcag aaatttcttc
    46981 aatctggcga cgtgactctt ccgagccaaa atagatagtc gcaccaaccg ccaccgcgcc
    47041 catgttgaaa gcctgctcca cgctggcata cagcgtttga tcgtaggtat tcgggtaact
    47101 tagcgtctcg ttgtgattaa gtttgacgag aaatggaatg cgatgcgcat agcgccgcga
    47161 taccgacgcc aacacgccgt aagttgatac cacacagtta cagcccgctt cgatcgccag
    47221 ttcaacaatg tttttcgggt caaagtagag cgggttagca gcaaatgaag ctccggcaga
    47281 gtgctcaacg ccctggtcaa ccggcagaat agaaagatat cctgtgccag ccagacgccc
    47341 ggtgttgtac aacgtctgca tattacgtaa caccgctggc gggcgattat tgtcaatcat
    47401 tacgcggtct acgtagtcat gtccggggag ataaagctgg tcagaaggaa ttgtcataca
    47461 acggtgctgt aaaaggttgt cggcgtcttt gccaagcaac tgcgcaatat ctgtcattac
    47521 tatgctcccg taaattccga ttggatatcg gctatggatt gtcctggccc gccttttgcg
    47581 ggcaatcata atcctggtcg ttacgggcga gtttttccat cttttggatg ttttttcagc
    47641 gttttctgtt ggctcgattc atcagaaaaa atgttagcgc ggtcaagttt tcaccgtaaa
    47701 ggcatttaaa aggtattatt aagtggtatt gtcatcgcgt accttacatt acctgtcatg
    47761 aaggaattaa aagatgaaaa caacagcaaa gctgtcgttc atgatgtttg ttgaatggtt
    47821 tatctggggc gcgtggtttg tgccattgtg gttgtggtta agtaaaagcg gttttagtgc
    47881 cggagaaatt ggctggtcgt atgcctgtac cgccattgcg gcgatcctgt cgccaattct
    47941 ggttggctcc atcactgacc gctttttctc ggcgcaaaaa gtgctggcgg tattgatgtt
    48001 cgcaggcgcg ctgctgatgt atttcgctgc gcaacagacc acttttgccg ggttctttcc
    48061 gttactgctg gcttactctc taacctatat gccgaccatt gcgctgacta acagcatcgc
    48121 ttttgccaac gtgccggatg tggagcgtga tttcccgcgc attcgtgtga tgggcactat
    48181 cggctggatt gcctccggtc tggcatgtgg tttcttgccg caaatactgg ggtatgccga
    48241 tatctcaccg actaacatcc cgctgctgat taccgccgga agttctgctc tgctcggtgt
    48301 gtttgcgttt ttcctgcccg gcacgccacc aaaaagcacc ggcaaaatgg atattaaagt
    48361 catgctcggc ctggatgcgc tgatcctgct gcgcgataaa aacttcctcg tctttttctt
    48421 ctgttcattc ctgtttgcga tgccactggc gttctattac atctttgcca acggttatct
    48481 gaccgaagtc ggcatgaaaa atgccaccgg ctggatgacg ctcggccagt tctctgaaat
    48541 attctttatg ctggcattac cgtttttcac taaacgcttt ggtatcaaaa aggtattatt
    48601 gcttggtctg gtcaccgctg cgatccgcta tggcttcttt atttacggta gtgcggatga
    48661 atatttcacc tacgcgttac tgttcctcgg tattttgctt cacggcgtaa gttacgattt
    48721 ttactacgtt accgcttaca tctatgtcga taaaaaagcc cccgtgcata tgcgtaccgc
    48781 cgcgcagggg ctgatcacgc tctgctgcca gggcttcggc agtttgctcg gctatcgtct
    48841 tggcggtgtg atgatggaaa agatgttcgc ttatcaggaa ccggtaaacg gactgacttt
    48901 caactggtcc gggatgtgga cttttggcgc ggtgatgatt gccattatcg ccgtgctgtt
    48961 catgattttt ttctgcgaat ccgacaacga aattacggct atcaaggtcg atgatcgcga
    49021 tattgcgttg acacaagggg aagttaaatg aaaacagaac gtattctcgg tgctctttat
    49081 gggcaggcgt taggggatgc gatggggatg ccctccgagc tttggccacg cagccgcgtt
    49141 aaagcacact ttggctggat tgaccgtttt ctgcctggac caaaggagaa taacgcggcc
    49201 tgttatttta accgcgccga attcaccgac gatacctcga tggcgctgtg tctggcggat
    49261 gcgttgctgg aacgtaaagg caagatcgat ccggatctga ttgggcgtaa tatcctcgac
    49321 tgggcgctgc gtttcgacgc cttcaacaaa aacgtgctag gtccgacctc gaagattgcg
    49381 cttaacgcca ttcgcgacgg taaacccgtt gctgaactgg aaaacaacgg cgtaaccaac
    49441 ggcgcagcga tgcgcgtctc gccattaggt tgtttgcttc cggcgcgcga tgttgattcc
    49501 tttattgatg atgtggcgct ggcgtcgagc ccgacccata aatccgatct ggcagttgca
    49561 ggtgcggtag tcatcgcatg ggcgatttct cgtgctattg acggagaaag ctggtcagcg
    49621 attgttgatt cactgccttc aattacgcga catgcccagc aaaaacgcac caccacgttc
    49681 agcgcctcac tggcggcgcg gctggagatt gcgctgaaaa ttgtgcgcaa tgccgacggc
    49741 accgaatccg ccagcgaaca gctttaccag gtcgttggcg caggtaccag cactattgag
    49801 tccgttccgt gcgccattgc gctggttgaa ctggcacaaa ccgacccgaa ccgttgcgcc
    49861 gtcctctgcg ctaaccttgg cggcgataca gacaccatcg gtgctatggc gacggcaatc
    49921 tgcggcgcgt tgcatggcat taacgctatc gatcctgcgt taaaggcgga gctggatgcg
    49981 gtaaatcagc ttgatttcaa ccgctatgcc acagcgctgg cgaaatatcg tcaacaacgg
    50041 gaggcggtat gagcggcgct cgattacaca cgctgctgcc tgaattaacc acgcgtcagt
    50101 cggtgatggt cgtcggcgcg gcggtcattg acgtgatcgc cgacgcttat gccctcccct
    50161 ggcgtgggtg cgatatcgaa ctgaaacagc agagcgttaa cgttggcggc tgcgcactga
    50221 atattgccgt ggcgttaaag cgcctcggca tcgaagcggg taatgccttg ccgctcggtc
    50281 agggggtatg ggcggagatt attcgcaacc ggatggcgaa agaggggtta atcagcctga
    50341 tcgataacgc cgaaggcgat aacggctggt gtctggcgct ggttgagccg gatggcgaac
    50401 gtacttttat gtcattcagc ggtgtggaaa atcagtggaa tcgccagtgg ctggcgcgat
    50461 taaccgttgc gcctggcagc ctgctctatt tttccggtta tcaactggcc tcgccctgcg
    50521 gcgaattgtt agtggaatgg ctggaagagc tgcaagacgt gacgccgttt atcgattttg
    50581 gcccacgtat tggcgatatc ccggacgcat tactggcgcg gatcatggcc tgtcgacctt
    50641 tagtgtcact caatcgtcaa gaggctgaga ttgccgccga acgttttgct ttatccgcag
    50701 agataacaac acttggcaag caatggcagg agaaatttgc cgcgccgttg atcgttcgtc
    50761 tcgataaaga aggcgcatgg tatttcagca acgacgcttc tggctgcatt ccggcatttc
    50821 caacgcaagt tgtcgacacc attggggcgg gcgacagtca tgccggtggc gtacttgccg
    50881 ggctggcctc tggtctgcca ctggcggatg ccgtattact gggcaatgca gtggcgtcat
    50941 gggttgtcgg gcatcggggc ggtgattgtg cgccaacgcg cgaggaacta ctcctcgcac
    51001 acaaaaacgt atagatcgct gcgacagtgg ctaatgctgt actcaatagg ccgttgctgt
    51061 tggtcaagcg ccacttgctt gatcaccagc accggtattt tgctatctaa ctgaatatgt
    51121 gactgaaact cggcatccgg catccgggcg ctaacgcggg aacgcgtacg ttgtgggtaa
    51181 atgtgctggc tgcggaaata gtcataaagt gaaatcccga tggcatcaac atcatgaatt
    51241 aaatgcgccg gaacccacga ttcctcaatc gataccgctt cttcatcgac atagcgaata
    51301 cgcttaagca aaaagacatc acttcctgct tcgaccgcca gctgctgcgc gacttcttcg
    51361 ggacatttca cgacacgctt gttaacccat agcgtatcgg gctttttccc gcgcaatacc
    51421 acctgctgag aaaaacctcg cgcttctttc aacgagtatt cgaagatgtt gttgatctgc
    51481 gtgccgtaac cgcgcgaacg cgtcaccaca ccctcttctt ccagcgcctg catcgccttg
    51541 cgcaccgtaa tgcgcgaaac gccggttaac tgacttaaat cacgctcacc gggcaaaata
    51601 ttgccatgct ccagcacccc gctgcgcacg gcatttttta ccgtttcggc aaacttaata
    51661 taaagcggcg tgttatccgc cgctaaaata cgctcgttca gttgggcaat cagctgggta
    51721 tgcgcttgtt ccatttgtct tgttcctgcc ggggagtctg gtgggagtat atcactgctt
    51781 cttatttaag gcgtttcttt aatcccatca acgaatgcct gcaactcctc caccgtgcca
    51841 ttaaatacat taaaatccac cggaccattg atgccatcta cctgtccacg gtcggaatgc
    51901 tgccagaagc gccaggccat gccgtcattg tccggacgac gttgataata gtgagccacc
    51961 caccacggat attcattgaa atagcccgcc agattggtgt gataaaaaac ggctcctgag
    52021 taaataatcg gcttttttcc cgtacttttt tcgaccattt ttagccactg acttactcgt
    52081 ttgcgtaatt cttttgccga taattttccc cgttcttcta cgtccagtac cgcagggaaa
    52141 tcaccttgtg agaaatccac cgtttgcaga aataatcgtg cctgaaccgg cgctgctacc
    52201 gacggggaga aataatgata cgccccgcgc agcaggccat tttcgcggct tagttgccag
    52261 ttacgcgaaa aatacgggtc caccagcttt tcgccttccg tcgccttaat aaaagcaaac
    52321 tgtaagcgga taccgttgtc gcgcattttt gccacccgct gccagtcgat ccgctcctgc
    52381 cagcgagaaa catctattcc gtgaatggtg taactggcag gaatgcgaat agcgaaagat
    52441 ttaaccgggc gataaccgta gaaatggatg tagtctgtaa tctgaactgc tgtcgaatag
    52501 aaaaaattca ccttctgacg cggataaatt gcaacgatgg aaattagccc aagagcgcat
    52561 aatagagagg tgagtttttt tcgactggta attcttagct gcattggttt catccctgaa
    52621 tgtcagtgcc aaaggctgac aataaccaaa gcagctatag tacggtgctc accaccaggc
    52681 gtggaagtgg tgaaccggac caataccgtg accaacttcc agcgtgtcgg cctgggctaa
    52741 tgctgatgaa agccagcttt ttgcctcctg tacggtgtca gcccagtttg tatggcgcgg
    52801 gcgtagtgca gccaacgccg cagagagcgt acaaccagtg ccgtgggtgt ttttggtcat
    52861 aatgcgcggt gcggtaaacc gttgttcacc ctcgcgggta aacagccagt ccgggctttg
    52921 ctcatcatcc agatgaccac ctttcattag cactgcgcca cagcccatcg ccaacagcga
    52981 tcgcccttgt tccagcattt cctgttcagt gcgcgcgtgt ggcgcgtcga gcaaggcggc
    53041 agcttcgggc aagtttggcg ttattaatga aacctgtggc aataatcgac tgcgcagcgt
    53101 agcaaccgcc gaaggtgaaa gcagcgggtc gccgcttttt gccagcataa cggtgtcgag
    53161 taccacgtat tggatctgat aacgttgcaa ccgttctgcc accgcttcaa caatatcggt
    53221 ttccgccaac ataccgattt tagtggtgtc gattcgcaca tcgctgaaca ccgaatcgag
    53281 ctgggcggcg acaaaatcag actcaatgcg atacaccgac tgtacgccac gggtattttg
    53341 cgccaccagt gcagtaataa ctgagcaacc ataagcgcca agtgccgaga aggttttaag
    53401 atcggcctga atccccgcac caccactcgg atcagtaccg gcaatcgtca gagcgttaat
    53461 tcgtttcatg cctgcacctc ctgcgtcaat tgccagagcg catcaaggaa atgtggaaca
    53521 aaactgcctg gcccctcgct tctggcgact gcgcgttctc cggcttgttt catccagtga
    53581 caggcagatg cgacattttc cagcgtatcg cctggtaacg cacagcaggc agcgacaacc
    53641 gccgataatg cacagccagt tcctaccact ttggtcatta acggatcgcc accgtgaata
    53701 ccaatgatac gatgtccatc ggtaacataa tccatctcgc cagtgaccac gacgattgcg
    53761 ccagtttccc gtgccagtgt ttgtgcagcg ggtatcgcgt tagctgcggc gtcagtggta
    53821 tccactcccc gtccaccatt agcaatgcca gctaatgcca tgatttccga agcattacca
    53881 cgtatagcgg ttggcttatg aggcagaagt tccagacaaa aacggcggcg ataatcgagt
    53941 gcaccaaccg ctactggatc aagcgtccag ggtgtttgag agctttttgc ttgctcaacg
    54001 gcggcacaca tcgactgtgc gcgtagctgc gtcagcgtgc caacgttaat cagcaacgca
    54061 ctagcaatag ccgcaaactg actggcctct tcggtttcga taaccatcgc tggcgatgca
    54121 ccgagcgcca gcaaggtatt ggcagtaaag gtttgcacca catcgttggt catgcagtgc
    54181 acaagagggg aatgttggtg aaaaaggtgt aacgcgtgcg cagattgcgc tgaactctgc
    54241 aggtcgactt gcatagtttg ctcctgccat aacgtgaaga agcaatgacc tggtggtccg
    54301 tgacttccct acgctggcat tatccagatc aggtaatacg ggtatttctc agccttcaca
    54361 aagaagggca ccccgagtca tttaaaacaa aatcaaaaga ttgcgattaa cgtcccgtta
    54421 atccctgttc tggatcatgc cagcggaaag ccaaagactc aaccctaaaa aatcctctgt
    54481 gtcccagttt gtgtctcacg ttgtggccca cagataaatg gcaggcacaa aaaaaccgcc
    54541 cataaggcgg cttaagtaaa ttattcagtc gtgaagattt aaatgaaatc tattttctgc
    54601 atacgctttc ttcatgcagt cgaagtgctt ttgccaattc caattggaat gcaaaggaaa
    54661 gacgggaacg gctgatcttg ataccgtcaa taatgatgta tgtagggtaa acagtgacca
    54721 tgcggcccaa gtattggaag tgtttagggt ggtttacttt atcgttattc attgtgtgcc
    54781 atccgtctgg gtgggggtaa tcccggcaac ggatgattga tctaaaatcg atcaaaatca
    54841 ataacttaac agatcaccaa atccaggggg gtgtacattg gcgagcctcc ctcagcctaa
    54901 cctatacacg accataaaat aggggcggat tccttccagt tgggtgggaa aatatctggg
    54961 agtttggata cagtagccag gcagggtaac cagacccata gatgagaatg tttattattc
    55021 ccaaatatcc aggcgagggg cctgctgtgg ctctctttac gcgccgctgt ggcgtcttct
    55081 ctcctctttc tatacaatcc catagctaac ccattgcgag gcttacagag cgttacagag
    55141 cgtccgtgtg aatattgcat aaatgaaagt ggtgaaaggt ccgatttggt caacttttag
    55201 acaactattt aacatatgcg caacaggtta gacctgtacc ctttcaatag gtccgcctcc
    55261 tgggcgctgt gtacgcccca taactgtggc tcttctgtac ccatccggga gccattccac
    55321 agagtaaact gtaaaactga tctgcctgtg agtaacgccc ttttcctgtg gataacttcc
    55381 acgcactaac cccataaact ccccttttga ggcgtccgcc tcttcaggcc ctttcttgtg
    55441 gcaaaaacac aaaaagggtt tagggcggtg gctaccgccc ctgtagatgg gaatgagtcg
    55501 cactcgaaag agggattgag aaccaattct caagtgataa tcgatctcat ttgtgttcga
    55561 agttttcccc agaaaaataa ttttggggtt aagcatgtaa aatgctacac acttgcactt
    55621 tgtaatgata attaattgac cataaaaaca aatagttaga agcgctgggg gagtcccact
    55681 agttacacaa ttgattgctt tttgagctaa aactgattct tttaccgggt atacaatcca
    55741 tttgatcgcc ctttaccaca tatttaaacg gatttaaagc catttattta gggggttagc
    55801 aaagtgttga ttccatggtt acagataaca aaaaagcccg ccgggggtta gccgggggcc
    55861 tgtggttggg ggtgtcaaac gtggttagtt cggcccgccc tgcattagca tgttgaaagc
    55921 ctgctgatta ttgctctcaa cttcagagcg taccagatcc ttaacctatc catcctgcac
    55981 tttcacatta acgttcacgt ttagcggctg gtttagccac gctggcggcg tcatcagtgg
    56041 attacctgcc atatcaactt taagctgtgg cgctgtaatc gtcggcgtag gaggtacata
    56101 agttggattg gagtaagagc ttacccaccc tgttttatct gctggtttgg caaagtctgg
    56161 attagtcgga aggtctggac tatggaggaa atcccacagt ttacccagcg ggctatcttt
    56221 tttcagattg cccccggcgg tggtgtcctg tccatacatg gcgtcgttag cgtcatgacc
    56281 ttatcccagg cataatcccc acctaccgaa ccgaccgcaa tgctccagcc aacaggaccg
    56341 ccagagagag ccgccgcgcc gcctaccttg ccaactttcg gaccccgacc cggtttgttt
    56401 ggcttgtctt taccttcccc accagatccg ccagtagtag cggcttcaat gccaccaata
    56461 gcggcgatcc ctttcatgac ccccagtaat ttggttagtg ggttaagcat ggtcaacaac
    56521 catttaaagg ctacgcccaa cttccagata gacccggcga acatagccac cccggcggcg
    56581 tatgctgcca tttcgcccca ggctttaagc tgctcaccag tgatccccat cttgctggcg
    56641 taatactcaa taatgcggaa ggtcaggatc acagtgtcat gcaactctgt aaacgcatct
    56701 gtggcaccct caacgatttt cccggcaata tgcccgaaca tctcaaaggc cccaccagag
    56761 ccgttaacag cggcgctgat agcgtcgaaa gcgctggtta attcgttgcc gaagccagaa
    56821 gcaaagaaga tgttcatcgc gttctgcata ctggtttgca ggcgctgcca gcttgcccgg
    56881 ttggacttca tagccttatc cagagcgccg ccgttacgag ctgcggcttt catctgttta
    56941 gccacttccg ggaggatgtc cttagagaac agcttccctt gttgcatcag gtcaaacaga
    57001 tccttttctg taaggttggt tgtccctttc atctggttga gagccttaac gaacagaccc
    57061 aca
//
LOCUS       DAJTYD010000007        46798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTYD010000007 DAJTYD010000000
VERSION     DAJTYD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.007"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..416
                     /locus_tag="OO585_000401"
     CDS             <1..416
                     /locus_tag="OO585_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6628098.1"
                     /translation="LRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLF
                     WRPEGLPGDPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKS
                     QRQTSWCQAIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            527..1537
                     /gene="pyrD"
                     /locus_tag="OO585_000402"
     CDS             527..1537
                     /gene="pyrD"
                     /locus_tag="OO585_000402"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6628099.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            1711..2253
                     /gene="zapC"
                     /locus_tag="OO585_000403"
     CDS             1711..2253
                     /gene="zapC"
                     /locus_tag="OO585_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6628100.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2250..3359)
                     /gene="ycbX"
                     /locus_tag="OO585_000404"
     CDS             complement(2250..3359)
                     /gene="ycbX"
                     /locus_tag="OO585_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6628101.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3603..5711
                     /gene="rlmKL"
                     /locus_tag="OO585_000405"
     CDS             3603..5711
                     /gene="rlmKL"
                     /locus_tag="OO585_000405"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6628102.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            5723..7630
                     /locus_tag="OO585_000406"
     CDS             5723..7630
                     /locus_tag="OO585_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6628103.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            7760..9013
                     /gene="pqiA"
                     /locus_tag="OO585_000407"
     CDS             7760..9013
                     /gene="pqiA"
                     /locus_tag="OO585_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6628104.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9018..10658
                     /gene="pqiB"
                     /locus_tag="OO585_000408"
     CDS             9018..10658
                     /gene="pqiB"
                     /locus_tag="OO585_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6628105.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            10655..11218
                     /gene="pqiC"
                     /locus_tag="OO585_000409"
     CDS             10655..11218
                     /gene="pqiC"
                     /locus_tag="OO585_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6628106.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11474..11641
                     /gene="rmf"
                     /locus_tag="OO585_000410"
     CDS             11474..11641
                     /gene="rmf"
                     /locus_tag="OO585_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6628107.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(11711..12229)
                     /gene="fabA"
                     /locus_tag="OO585_000411"
     CDS             complement(11711..12229)
                     /gene="fabA"
                     /locus_tag="OO585_000411"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6628108.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12298..14058)
                     /locus_tag="OO585_000412"
     CDS             complement(12298..14058)
                     /locus_tag="OO585_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6628109.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPNLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14244..14696
                     /gene="matP"
                     /locus_tag="OO585_000413"
     CDS             14244..14696
                     /gene="matP"
                     /locus_tag="OO585_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6628110.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(14772..15812)
                     /gene="ompA"
                     /locus_tag="OO585_000414"
     CDS             complement(14772..15812)
                     /gene="ompA"
                     /locus_tag="OO585_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6628111.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16169..16678)
                     /gene="sulA"
                     /locus_tag="OO585_000415"
     CDS             complement(16169..16678)
                     /gene="sulA"
                     /locus_tag="OO585_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6628112.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            16897..17526
                     /gene="sxy"
                     /locus_tag="OO585_000416"
     CDS             16897..17526
                     /gene="sxy"
                     /locus_tag="OO585_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6628113.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17489..19651)
                     /gene="yccS"
                     /locus_tag="OO585_000417"
     CDS             complement(17489..19651)
                     /gene="yccS"
                     /locus_tag="OO585_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6628114.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(19661..20107)
                     /locus_tag="OO585_000418"
     CDS             complement(19661..20107)
                     /locus_tag="OO585_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6628115.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20230..22290
                     /gene="helD"
                     /locus_tag="OO585_000419"
     CDS             20230..22290
                     /gene="helD"
                     /locus_tag="OO585_000419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6628116.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPLPVNRLEEFDNCREAWRK
                     CQAWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLA
                     GAGSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITART
                     FHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEE
                     MQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIK
                     LMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAA
                     LLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNS
                     RIGKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERIL
                     ILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAAR
                     ESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPV
                     ARKP"
     gene            complement(22322..22780)
                     /gene="mgsA"
                     /locus_tag="OO585_000420"
     CDS             complement(22322..22780)
                     /gene="mgsA"
                     /locus_tag="OO585_000420"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6628117.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVAANVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(22876..23538)
                     /locus_tag="OO585_000421"
     CDS             complement(22876..23538)
                     /locus_tag="OO585_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6628118.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            23711..24124
                     /locus_tag="OO585_000422"
     CDS             23711..24124
                     /locus_tag="OO585_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6628119.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24169..24486)
                     /gene="hspQ"
                     /locus_tag="OO585_000423"
     CDS             complement(24169..24486)
                     /gene="hspQ"
                     /locus_tag="OO585_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6628120.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24544..25734)
                     /gene="rlmI"
                     /locus_tag="OO585_000424"
     CDS             complement(24544..25734)
                     /gene="rlmI"
                     /locus_tag="OO585_000424"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6628121.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            25829..26107
                     /gene="yccX"
                     /locus_tag="OO585_000425"
     CDS             25829..26107
                     /gene="yccX"
                     /locus_tag="OO585_000425"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6628122.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26104..26433)
                     /gene="tusE"
                     /locus_tag="OO585_000426"
     CDS             complement(26104..26433)
                     /gene="tusE"
                     /locus_tag="OO585_000426"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6628123.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26524..27183)
                     /gene="yccA"
                     /locus_tag="OO585_000427"
     CDS             complement(26524..27183)
                     /gene="yccA"
                     /locus_tag="OO585_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6628124.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27390..27477)
                     /locus_tag="OO585_000428"
     tRNA            complement(27390..27477)
                     /locus_tag="OO585_000428"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27441..27443),aa:Ser,seq:tga)
     gene            27904..29022
                     /gene="hyaA"
                     /locus_tag="OO585_000429"
     CDS             27904..29022
                     /gene="hyaA"
                     /locus_tag="OO585_000429"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6628125.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29019..30812
                     /gene="hyaB"
                     /locus_tag="OO585_000430"
     CDS             29019..30812
                     /gene="hyaB"
                     /locus_tag="OO585_000430"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6628126.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMINLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            30831..31538
                     /gene="hyaC"
                     /locus_tag="OO585_000431"
     CDS             30831..31538
                     /gene="hyaC"
                     /locus_tag="OO585_000431"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6628127.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31535..32122
                     /locus_tag="OO585_000432"
     CDS             31535..32122
                     /locus_tag="OO585_000432"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6628128.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32119..32517
                     /gene="hyaE"
                     /locus_tag="OO585_000433"
     CDS             32119..32517
                     /gene="hyaE"
                     /locus_tag="OO585_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6628129.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32514..33371
                     /locus_tag="OO585_000434"
     CDS             32514..33371
                     /locus_tag="OO585_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6628130.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33505..35049
                     /gene="appC"
                     /locus_tag="OO585_000435"
     CDS             33505..35049
                     /gene="appC"
                     /locus_tag="OO585_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6628131.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35061..36197
                     /gene="appB"
                     /locus_tag="OO585_000436"
     CDS             35061..36197
                     /gene="appB"
                     /locus_tag="OO585_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6628132.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36210..36302
                     /gene="cbdX"
                     /locus_tag="OO585_000437"
     CDS             36210..36302
                     /gene="cbdX"
                     /locus_tag="OO585_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6628133.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36382..37680
                     /gene="appA"
                     /locus_tag="OO585_000438"
     CDS             36382..37680
                     /gene="appA"
                     /locus_tag="OO585_000438"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6628134.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNV
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(37795..39975)
                     /gene="etk"
                     /locus_tag="OO585_000439"
     CDS             complement(37795..39975)
                     /gene="etk"
                     /locus_tag="OO585_000439"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6628135.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLHDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(39995..40441)
                     /gene="etp"
                     /locus_tag="OO585_000440"
                     /gene_synonym="yccY"
     CDS             complement(39995..40441)
                     /gene="etp"
                     /locus_tag="OO585_000440"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6628136.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40429..41568)
                     /locus_tag="OO585_000441"
     CDS             complement(40429..41568)
                     /locus_tag="OO585_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6628137.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41614..43710)
                     /locus_tag="OO585_000442"
     CDS             complement(41614..43710)
                     /locus_tag="OO585_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6628138.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(43710..44456)
                     /locus_tag="OO585_000443"
     CDS             complement(43710..44456)
                     /locus_tag="OO585_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6628139.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44453..45097)
                     /gene="gfcB"
                     /locus_tag="OO585_000444"
     CDS             complement(44453..45097)
                     /gene="gfcB"
                     /locus_tag="OO585_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6628140.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45204..45509)
                     /locus_tag="OO585_000445"
     CDS             complement(45204..45509)
                     /locus_tag="OO585_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCR6628141.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(45951..46163)
                     /gene="cspH"
                     /locus_tag="OO585_000446"
     CDS             complement(45951..46163)
                     /gene="cspH"
                     /locus_tag="OO585_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6628142.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46449..46661
                     /gene="cspG"
                     /locus_tag="OO585_000447"
     CDS             46449..46661
                     /gene="cspG"
                     /locus_tag="OO585_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6628143.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            46672..>46798
                     /locus_tag="OO585_000448"
     CDS             46672..>46798
                     /locus_tag="OO585_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6628144.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMI"
BASE COUNT        11529 a        11622 c        12297 g        11350 t
ORIGIN      
        1 tgctgagaac ggaaagcgac atcttgcctg tggatcgcga aacgctattt gagttaagca
       61 ttgccagcgt gccatccggc aaagttgaaa atcagagcgt aaaagtggcg atgcgctcgg
      121 tatttaaact gttctggcga cccgaaggtt tgccgggcga cccgctggaa gcttatcaac
      181 aattacgctg gacacggaat tcgcagggtg tacaactcac taacccaacg ccttactaca
      241 ttaacctgat tcaggtgagc gtaaatggta aagcgttaag taatgcggga gttgtgccgc
      301 ctaaaagcca gcgtcagaca agctggtgtc aggcaatcgc accgtgtcat gtcgcctggc
      361 gggcgataaa tgattacgac gggttgagtg caaagaagga gcaaaatctg ccctgaaaca
      421 ggttcggaaa acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg tccgaactcg
      481 cacataatac gcccccggtt tgcacaccgg gaatccagga gagttcatgt actacccctt
      541 cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct catgagttta cttttcagca
      601 attacgccgt attacaggaa cgccgtttga agcactggtg cggcagaaag tgcctgcgaa
      661 acctgttaac tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag ccggtcttga
      721 taaagacggg gagtgcattg acgcgttagg cgcgatggga tttggatcga tcgagatcgg
      781 taccgtcacg ccacgtccac agccaggtaa tgacaagccg cgtctctttc gtctggtaga
      841 tgccgaaggt ttgatcaacc gtatgggctt taataatctt ggcgttgata acctcgtaga
      901 gaacgtaaaa aaggcccatt atgatggcgt cctgggtatt aacatcggca aaaataaaga
      961 tacgccagtg gagcagggca aagatgacta tctgatttgt atggaaaaaa tctatgccta
     1021 tgcgggatat atcgccatca atatttcatc gccgaatacc ccaggattac gcacgctgca
     1081 atatggtgaa gcgctggatg atctcttaac cgcgattaaa aataagcaaa atgatttgca
     1141 agcgatgcac cataaatatg tgccgatcgc agtgaagatc gcgccggatc tttctgaaga
     1201 agaattgatc caggttgccg atagtttagt tcgccataat attgatggcg ttattgcaac
     1261 caataccaca ctcgatcgtt ctcttgttca gggaatgaaa aattgcgatc aaaccggtgg
     1321 cttaagtggt cgtccgcttc agttaaaaag caccgaaatt attcgccgct tgtcactgga
     1381 attaaacggt cgcttaccga tcatcggtgt tggcggcatc gactcggtta tcgctgcgcg
     1441 tgaaaagatt gctgcgggtg cctcactggt gcaaatttat tctggtttta tttttaaagg
     1501 tccgccgctg attaaagaaa tcgttaccca tatctaatta tttcttcgaa ttcctcttta
     1561 taagataatc agggctttat tttcagccct ggttgtttta tattcatcgc tgttgcttat
     1621 ttagacattt tgtactttta ttgttgaggt tattaagcga agcgacaatg gattgtgttg
     1681 ctgcggtttt atagggtagg ggagaggcag atgcgaatta aaccagacga taactggcgt
     1741 tggtattacg atgaagagca cgatcgaatg atgctcgatt tagccaatgg tatgctattt
     1801 cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct tttctcccgc aggcttttgc
     1861 gttgacgacg ccgcgctcta tttctctttt gaagaaaagt gccgcgattt taatttatct
     1921 aaagaacaaa aagccgaact ggtgctgaat gtactggtag caattcgcta tctcaaaccg
     1981 caaatgccga aaagctggca ttttgtttcc catggtgaaa tgtgggtgcc catgccgggc
     2041 gatgcggcct gcgtttggtt gagcgatact cacgagcagg tcaatttgct ggtcgttgaa
     2101 tctggcgaaa atgccgcact atgcctgctg gcacaaccct gcgttgttat tgcgggtcgc
     2161 gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc gcaggttaat
     2221 gttgacagct tcagcctcga acaggcagtc taacgcgcca acttaagtgc agtcttcggt
     2281 acacagctac agcaaagaat ggtgccatca tcgcccattg ctgatttttt cagcggcgag
     2341 acttcgcctt ctaaaatctg aacacggcaa cttccacaaa tgcccgcgcg gcaagaataa
     2401 gggatacgaa ttccctgatt ttctaattgc tccagcaaca cctgttggtt atttccacga
     2461 aatgcctgtc cctgccagtc aatatctaca tttgcgtccg gttgttgcgt gatgttggcg
     2521 gtatcatcag cggcagctgc gccgtaaatt tttgccggag ccgttgccag aatttccacc
     2581 tcatcgccaa cgcgaatcac gccgctatta cgggcaatta aattctgacc aaaatcgaca
     2641 tcgccgttat cctgggcagt gcggaaagat tgcaatgttt ttaatggttc gcctgccgga
     2701 tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac agcggctaca aggtttaacc
     2761 acatcaaaca ccacatcacc aatgcgaatc actttccagc gatcttcttc ccaggctgac
     2821 gcgccggaaa ccaccagatt ggggcggaat tgctccattt ttacactggc cggacaacgt
     2881 tgttggagat cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc atcagcaaat
     2941 gacagaggta cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc cacccagcgt
     3001 aattgcactt cgcgggagaa aaatccactt agccatttgt tgatcgcgtc tggcgcaatt
     3061 cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc gaaatcagca
     3121 aaacgaacat atgcgctact gccatctggt gcggtgagat gcaagccatc atgcacaggt
     3181 gaaggagtaa accgtaccat ctggggaaac tggcgagcgg taataaacgt accgtcaggt
     3241 tccgtgatca taaagatgcg atcgaaggcc agaccactga catcagccag agcatgtgta
     3301 agaccaatgc cgcgcatcga tttaacagga tgaataaaaa gccgggttaa tgtcgccacg
     3361 gtgcggtcct caaatgaaaa taagccctca actttatgac atgacgcgct tattagctat
     3421 aatgcgcaac aattttctta gcgcctgtcc cattaggcta ttttatttgt cagtttggcc
     3481 ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc cgttttctcc gcgctgtcca
     3541 tgtccagact gactgcaaca attacgccta ctgcggtagg ctcttagagt aaaagtgacg
     3601 atatgaattc tctgtttgcc agtacggccc gtgggctgga agagctgtta aaaactgaac
     3661 tggaaaacct gggggccgtt gaatgccagg tggttcaggg tggggtccat ttcaagggcg
     3721 acacacggct tgtttaccag agcctgatgt ggagccgcct ggcctcgcgt attatgttgc
     3781 cgctgggcga gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt caggcgatca
     3841 actggacaga gatgtttaat cctggcgcga ccttcgctgt ccacttcagt ggtttgaatg
     3901 acaccatccg caacagtcag tacggcgcga tgaaagtgaa agacgcgatc gtcgatgctt
     3961 tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat atccgcgtta
     4021 acgtctggct gcataaagaa accgccagta tcgctcttga tctgagtggt gatggtttac
     4081 atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc ctggcagccg
     4141 ctattgtgat gcgatccggc tggcagcctg gaacaccgct gctcgatccg atgtgtggtt
     4201 ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga tcgcgcaccc ggcttgcacc
     4261 gtgggcgttg gggctttagc ggctgggcgc agcatgatga agctatctgg caggaagtga
     4321 aagcggaagc gcaaactcgc gcccgtaaag gcctagctga gtatagctct catttttacg
     4381 gttcggacag cgacgcacgg gtgattcaac gtgcacgcac taacgcccgt cttgcgggga
     4441 ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca actgaccaat ccgctgccga
     4501 aagggccgta cggcacagtg ttgagcaacc cgccatacgg ggaacgtctg gacagcgaac
     4561 cggcgctgat tgcgctgcat agcctgctgg gtcggatcat gaaaaaccag tttggtggct
     4621 ggaatctctc tttgtttagt gcctcgccgg atctgctaag ctgcttgcag ctgcgtgcag
     4681 acaaacagta caaggcgaaa aacggcccgc tggactgcgt acagaaaaat taccacgttg
     4741 ccgaaagcac tccagacagc aaaccggcga tggtagcgga agactacgcc aaccgcctgc
     4801 gtaagaacct caaaaaattc gagaagtggg ctcgccagga aggaattgaa tgttaccgcc
     4861 tgtatgacgc cgatctgccg gaatataacg ttgccgttga ccgttattcc gactgggtgg
     4921 tggtgcagga gtatgcaccg ccaaaaacta ttgatgctca caaagcgcgt cagcgtctgt
     4981 tcgatattat cgctgcaacc atttcggtac tggggattgc gccaaacaaa ctggtgctga
     5041 aaacccgtga acgccagaag ggcaaaaacc agtaccagaa actgggcgag aagggcgagt
     5101 ttcttgaagt taccgaatat aacgctcact tgtgggtgaa cctgacggat tatctcgata
     5161 ctggtctgtt cctcgatcac cgtatcgccc gccgtatgct cggtcagatg agcaaaggca
     5221 aagatttcct caacctgttc tcttataccg gtagcgccac cgtgcatgcg ggattaggcg
     5281 gcgcgctcag caccaccacc gtggatatgt cgcgtactta tctggagtgg gcagaacgca
     5341 acctgcgtct gaatggccta accgggcgtg cgcatcgcct gattcaggcc gattgcctgg
     5401 cgtggctgcg tgaggcaaat gaacagtttg atctgatctt tatcgatccg ccaaccttct
     5461 ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg cgatcatctg gcgctgatga
     5521 aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac aacaaacgtg
     5581 gcttccgtat ggatctcgac ggcctggcaa aactgggact gaaagcacaa gaaattacgc
     5641 aaaaaacgct ctcccaggat ttcgcccgta accgccagat ccacaactgc tggctgatta
     5701 ccgcagcctg aaaggaatag taatgtcatt aatcagtatg catggcgcat ggctgtcgtt
     5761 cagcgacgcg ccgcttctcg ataacgcaga actgcatatc gaagataacg aacgtgtttg
     5821 tctggtgggc cgcaacggcg cgggcaaatc gacgttaatg aaaatcctca accgtgaaca
     5881 agggctggat gacggtcgca ttatttacga gcaagatttg attgtagctc gtctgcaaca
     5941 ggatccgccg cgtaacgttg agggtagcgt ttatgatttc gttgccgaag gcattgaaga
     6001 acaagcggaa tatctgaaac gctatcacga tatttcgcgc ctggtgatga acgacccgag
     6061 cgagaaaaat ctcaacgaac tggcgaaggt tcaggaacag ttggatcacc acaacctgtg
     6121 gcagctggaa aaccgcatca acgaagtgct ggcgcaactg gggttagatc ctaatgttgc
     6181 gctgtcgtcg ctttccggcg gctggttgcg taaagcggca ttaggacgcg cgctggtgag
     6241 taatccgcgc gtgctgttgc ttgatgaacc gacaaaccac ctggatattg aaaccatcga
     6301 ctggctggaa gggttcctta aaaccttcaa tgggacgatt attttcatct cccacgaccg
     6361 ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca agctggtgac
     6421 ctatccaggg aattacgacc agtacctgct tgaaaaagaa gaagccctgc gcgtggaaga
     6481 actacaaaat gccgagttcg atcgcaaact ggcgcaggaa gaggtgtgga tccgccaggg
     6541 gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag caatgcgtcg
     6601 cgagcgtggt gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg aagaggccag
     6661 ccgctccggt aagatcgttt tcgaaatgga agacgtttgc taccaggttg acggtaagca
     6721 actggtgaaa gatttttcgg cccaggttct acgtggcgac aaaattgtcc tgattggtcc
     6781 gaatgggtgc ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc aagcggacag
     6841 cgggcgtatt cacgttggca ccaaactgga agtggcttat ttcgatcagc accgcgcgga
     6901 actggatccc gataaaacgg tgatggataa cattgccgaa ggtaagcaag aggtgatggt
     6961 taacggcaag ccacgccacg tattgagcta tttgcaggac tttctgttcc atccgaaacg
     7021 ggcgatgacg ccggtacgtg cgctttctgg cggtgagcgg aaccgcttgc tgctggcgcg
     7081 tttgttcctc aaaccaagca acttattgat tcttgacgaa ccgaccaacg atcttgatgt
     7141 cgaaacgctg gaactgctgg aagaactgat cgacagctat cagggcacgg tattgctggt
     7201 tagccacgat cgtcagtttg tcgataacac cgttacagaa tgttggatct tcgaaggcgg
     7261 cggtaaaatt ggtcgttatg tcggcggtta tcatgatgcc cgtggtcagc aagagcagta
     7321 tgtggcgctc aaacagcctg cggtgaaaaa aaccgaagaa gccgccgcgg caaaagcaga
     7381 aactgtaaaa cgcagcagta gcaaactaag ctataaattg cagcgcgaac tggagcagct
     7441 accgcaattg ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc aggtggcgga
     7501 tgcttccttc ttcagtcagc cacatgagca gacacaaaaa gtgctcgccg atatggctgc
     7561 tgcagagcag gagctggagc aagcctttga acgctgggag tatcttgaag cgttaaaaaa
     7621 tggtggctga tcgcaaaata gtcgatttaa acagagacag ctgacaacgg taaaattgcc
     7681 tgatgaactg ggtttatcag gcatgaaaac caacactttg ttagtcatct gtaaaatagc
     7741 gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca tcctgtgctc
     7801 gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag cggcatgtcc
     7861 ccggtgtggc acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc cgaccgccta
     7921 tgcgttggct gcactgttca tgctgttgct gtccaacttg tttccttttg tgaatatgaa
     7981 cgttgcagga gttaccagtg aaattacatt actggaaatt cccggcgtgc ttttttctga
     8041 ggactacgcc agcctcggca cctttttcct gttgtttgtg caactggttc ccgcgttttg
     8101 tctgataacc attctgttac tggtgaatcg cgcggaatta ccggtccgtt taaaagagca
     8161 actggcacgg gtgctttttc aactcaaaac ctggggaatg gcggagattt tcctcgcggg
     8221 tgtgctggtc agtttcgtta aactgatggc ttacggcagc attggggtag gcagcagctt
     8281 tctcccctgg tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg ttgatcgtcg
     8341 ctggttatgg gacgacatcg ccccgatgcc agaactgcgc cagccgctaa aaccaggcgt
     8401 cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc ttcctgctga
     8461 tgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa acagcctgca
     8521 gtggacactc gcgctgcttg taacgtccat catgctgtat cttccggcta atattttgcc
     8581 catcatggtg acggatttat taggctcgaa gatgccgtcg acgattctcg ctggggtcat
     8641 tctgttatgg agcgaaggct cttatcccgt cgctgcggtg atctttctgg ccagtattat
     8701 ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca aagggcatgg
     8761 caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg taggccgctg
     8821 gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta tgggaggttt
     8881 aatgagtatt tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca taatgacaat
     8941 gttttctgct atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg aatcagagca
     9001 tgaggagtcc tgacagtatg gaatctaata atggggaagc caaaatccag aaagtgaaga
     9061 actggtctcc cgtgtggata tttcctatcg tcacggcgct cattggggcc tgggttctat
     9121 tttatcatta cagccatcag ggaccggaag tgaccctgat caccgcgaat gcggaaggaa
     9181 ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg gttgaaagcg
     9241 ccacactggc tgatgatttg acgcacgttg aaatcaaagc gcggctgaat tccggtatgg
     9301 aaaaattgct gcataaagac accgtctttt gggtggtgaa accgcagatt ggtcgcgaag
     9361 ggattagcgg cctgggaacg ctgctgtctg gagtttatat cgaactgcag ccaggcgcga
     9421 aaggcagcaa aatggataaa tacgatttgc tggactcgcc accgttggcc ccgcctgatg
     9481 cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg ccaggagatc
     9541 cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac cagcaccttc gatacgcaaa
     9601 aacgcaatat cagttatcaa ctgttcatca atgcacctta tgaccgactg gtgaccagca
     9661 atgttcgctt ctggaaagat agtggcattg cggttgatct gacgtcagcg gggatgcgtg
     9721 tggagatggg ctcattgaca acgctgctta gtggcggtgt tagctttgat gtgccggaag
     9781 gtctggattt agggcagcca gtggcaccga aaacagcttt cgttttgtat gatgatcaga
     9841 agagcattca ggattcgttg tacaccgatc acattgatta tctgatgttc tttaaagatt
     9901 cggtacgcgg tctgcaaccg ggagctccgg tagaattccg gggtattcgc ctgggtaccg
     9961 taagcaaagt gccattcttt gcgccgaata tgcgtcagac atttaacgat gattaccgta
    10021 ttccggtact gattcgtatc gagccagagc ggctgaaaat gcagcttggc gaaaatacgg
    10081 atgttgttga gcaccttggc gaattgttga aacgtggttt acgcggatcg ctgaaaaccg
    10141 gaaacctggt cactggtgca ctgtatgttg atctcgattt ctatccaaat acgcctgcaa
    10201 taaccggtat tcgtgaattt aatggttatc agattatccc gactgttagc ggcggcctgg
    10261 cgcaaatcca gcaacgactg atggaagcgt tggataagat caacaaactg ccattgaatc
    10321 cgatgattga acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg aaaaacctgc
    10381 aaacgacgct ggatagcatg aacaagatcc tcgctagcca gtcgatgcag cagttgccga
    10441 cggatatgca gtcaacgttg cgtgaattga atcgcagcat gcagggcttc cagcctggct
    10501 ccgcagccta caacaagatg gtggcggata tgcagcgcct tgatcaggtg ttgcgagaac
    10561 tgcaaccggt gctgaaaacg ctcaatgaga agagtaacgc gctggtattt gaagcgaagg
    10621 acaaaaaaga tccagagccg aagagggcga aacaatgaaa aagtggctag tgacgattgc
    10681 agcactgtgg ctggccggat gcagctccgg cgaaattaat aaaaactatt accagttacc
    10741 tgtggtgcag agcggtacac aaagtaccgc cagccagggc aatcgtctgt tatgggtaga
    10801 gcaggtcact gttcctgact atctggcggg gaatggtgtg gtttatcaaa ccagtgatgt
    10861 gaagtatgtg attgccaaca acaacttgtg ggccagcccg ttggatcaac agttgcgcaa
    10921 caccctggtt gccaacctga gtacgcaact gcccggctgg gtggttgcct cccagcctct
    10981 gggaagcgtc caggacacgc tcaatgttac cgtaacggag tttaacggtc gctatgatgg
    11041 caaggtcatt gtcagtggtg agtggctgtt gaaccaccag ggacaactga tcaaacgtcc
    11101 gttccgtctg gaaggagtgc aaactcagga tggttacgat gagatggtta aagtgctggc
    11161 cggtgtctgg agtcaggaag ccgcttctat tgcacaagag ataaagcgtc taccttaatt
    11221 ataaagattt gtaagtataa ccgtctccgg tatgttgcct gaggcggttt ttttgtttct
    11281 aacgtgcgga aaaatttgtt cctcttcaca ttttttgtac aaccgacatg cccgtgtagc
    11341 tcacaaatat gacagtggcg tgaattttgc gcattgacgg cagttatgat tcgcggtatt
    11401 gcttaactgt gattgcacat ttagtaatca ctgttttctt ttccaccaga aaccagtatg
    11461 agggaaacga ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac atcaacgtgg
    11521 ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga cgctgaatca
    11581 aaggtcacaa tggctgggag gctggcgaga agccatggcg gacagggtag taatggcctg
    11641 attctgtctc tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt gatactctgt
    11701 ctgatgataa tcagaaggca gacgtatcct ggaacagacc gactttcagg tcgctggcgg
    11761 tatagatcag acgaccatca accagcactt cgccatctgc cagacccata atcagacgac
    11821 gattaacaat gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc ggcagtacct
    11881 gaccagtgaa tttcacttcg ccaacgccca gcgcgcggcc tttaccttcg ccgcccagcc
    11941 agccgaggta gaaccctact agctgccaca ttgcgtccag gcccaggcat cccggcataa
    12001 ccggatcgcc aatgaagtgg catccgaaga accacagatc cggattgata tccagttctg
    12061 cttcaacata ccctttgtcg aagttaccac ccgtttcggt cattttgacc acacggtcca
    12121 tcatcagcat gttcggtgct ggcaattgcg ggcctttagc gccaaacagt tcaccgcgac
    12181 cagaggcaag aaggtcttct tttgtatagg attcgcgttt atctaccatg ttctctgtaa
    12241 gccttatttt attgaagcac gcaggatagc taacacgtgt acgctgaaca agtccgatca
    12301 gttcggaata aaccagttca gccaacgtaa tggccatgga aaacggtgac gtccttcctg
    12361 ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt ggccttcgcc
    12421 atcccacacc agatttaata ataacggcag tgcgtcagtc acatcgtcta ctgcccagat
    12481 ggtgaatttg ccttcttcta ccgctttcac cagttcactg tgaagactta aatggcgaac
    12541 gttagctgtg gggataatga caccttgttt cccggttaac tcacgttgct ggcaaatagc
    12601 aaagaagcct tcgattttct catttaaacc accgaccggc tgggcgcgac cgaactgatc
    12661 gactgaacct gtgatagcga tactctgatt caccggcaca tcggcgaggg cgcttatcag
    12721 ggcgcagagt tcagccatcg aggcactatc tccatcaact tcactgtatg actgctcaaa
    12781 tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt agttccgaca tcaggaacgc
    12841 ttgcatgatc atcatccctt tcgcatggat attgccgcca agttccgctt tgcgttcgat
    12901 gtcggtgaat tcaccatcgc caatatgcac aacgcagcta atgcgagaag gttcgccaaa
    12961 agcgcgtgga tgacccggaa attcaatgac cgaaagggcg ttaatttgcc cgatgcgttc
    13021 gccttcggtt tcaatcagga tttgctcctg aaggatctca tcctgcatac gttcagcgag
    13081 gaaaccttcg cgccattcac gctgctgcag cattaagttt agctgctcgc cggagaaggt
    13141 gtcgccatca cacagggagg cgacctcttt acactggcgg aggatccact gcgggctaag
    13201 cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg cggataagta ccggccaggc
    13261 atccgctccc ggggcaggta agtgattatg tctggcggta aatgtcaccc agcgacacca
    13321 ctggcttact gactccgcat cgacaatctg cagagtatct tcaaattcgc tataaatagc
    13381 ctgctctgaa agctctggct ccatctcctg gaaatcagcc aatgattcgc gttcgcctac
    13441 cagaatgact ttcagcttca atggcatcga aggcacagag acagggagag ggcgcgactc
    13501 atcaaacgca acccagtcaa aacgctcgcg gttaacgata tttttcagcc gcatccacag
    13561 cagaggttgc gccagcagtg tacgcaaaga gatgatgaga ataccgccat ttgcctgatg
    13621 caccagacca ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc caaagagttg
    13681 ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat tatcatctgc
    13741 actcactgcg tgacgtaagc ggatggagtg gccggaaacc tcatagtgat cgcccaccag
    13801 ttggcctgca tcgctttgta gcgtacgcgc ggcatcggca ataagattca gatactcaga
    13861 ctcttccggg gccttagcca gcataaaaga ggaggatgct cgcgtatgca gcaactgctc
    13921 cagcgcaaat tgcagccgcg gttgagtatc actgaataaa ggatcgtttt cgtcaatcaa
    13981 atttggctga gcaaatattt cctgatagct atcggtatca ggaaccaggt cacgccatgc
    14041 aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg cgattatacc
    14101 gtaaccggca ctacagcaca cgtagaaagc accgacaata ctcctggcat gggcgttaaa
    14161 gctcacagga tggagattct tttcttcact ggcctaaaaa gctgatattc tgtaaagagt
    14221 tacacggtaa cattgagatc gctatgaaat atcaacaact tgaaaatctt gaaagcggtt
    14281 ggaaatggaa gtatctggtg aagaagcatc gcgaagggga gttaattacc cgttacatag
    14341 aagccagtgc cgcccaggaa gccgtcgatg tgttgctctc gctggaaaat gaacccgtgc
    14401 tggtaaatgg ctggattgac aagcatatga atccggaact ggtcaatcgc atgaagcaaa
    14461 ccattcgggc cagacggaag cgccatttta atgcggaaca ccagcatacg cgcaaaaagt
    14521 cgatcgatct ggaatttatt gtctggcaac gtctggctgg tcttgcgcag cgtcgcggta
    14581 aaacgctttc tgaaacgatt gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg
    14641 cgaataaaat gtcttctctg aagctggatc tgcaggcatt gctgggtaag gaataactga
    14701 cgaaagtcag ttcaatttac taaaggcaaa aaaaaccccg cagcagcggg gtttttctac
    14761 cagacgataa cttaagcctg cggctgagtt acaacgtctt tgataccttt aacttcgatc
    14821 tctacgcgac gatccggagc caggcagtcg atcagtgcag cacgctgttt cacgttgtca
    14881 caggtgttgc cagtaaccgg gttggattcg cccataccac gtgcggagat cttgtctgcc
    14941 gggatacctt tggagatcag gtaatcaaca acagactgag cacggcgctc ggacagaccc
    15001 tggttgtaag cgtcagaacc gatgcggtcg gtgtaaccca gaacaactac ggaaccgtct
    15061 ttcggatcca ggttgctcag ctggctgtac agctgatcca gagcagcctg accttccggt
    15121 ttcagggttg ctttgttgaa gttgaacaga acgtcagact tcagagtgaa gtgcttggtc
    15181 tgtacttccg gtgccggagc tggagccgga gcaactactg gagctgcttc gccctgaccg
    15241 aaacggtagg aaacacccaa gctcagcatg ccgttgtccg gacgagtgcc gatggtgtgt
    15301 gcgtcaccga tgttgttggt ccactggtat tccagacggg tagcgatttc aggagtgatc
    15361 gcgtactcaa caccgccagc gaagaccgga gaaacgccgg tgtcgtggtt tttaccataa
    15421 acgttggatt tagtgtctgc acgccatacc ataccaccca gacgagtgta gatgtccagg
    15481 tcgtcagtga ttgggtaacc cagtttagcg gtcagttgaa cgccctgagc tttgtatgca
    15541 ccgttttcaa cgctgccttt gtacggcata cgacctaacc agtcgtaacc catttcaaag
    15601 ccaacatacg ggttaacctg gtaaccacca aaagcaccag cgcccagttg gttttcatgg
    15661 gtcgggccat tgttgttgat gaaaccagtg tcatggtact gggaccagcc cagtttagca
    15721 ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa accagccagt
    15781 gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc atccaaaata
    15841 cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg atctgctcaa
    15901 tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca
    15961 acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat
    16021 aaggtatgtt taatcttttt tgtcagcgac aatttacaga agaaaatcgc ggaaaccgct
    16081 tcagacaagc ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggcgacaaaa
    16141 aaagttccag gattaatcct aaatttactt aatgatacaa attagagtga atttttagcc
    16201 cggaaagttg tctcgtggcg tgagaggatg cgcttaccgg acgcataata aaccccatag
    16261 cgttaccttc atttgccgca tcaacaagtt cagcatgctc ttcttcagtc aaatcatctg
    16321 ccaaccaacc gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca
    16381 cagtgtggca aggggagagc tggctaatct gcattacttt cgttaagggt agcccagatg
    16441 cctgaaccca ttcccgactc agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt
    16501 gctgaccgag ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg ggctgatctt
    16561 cgcgatagac aacttcactg ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa
    16621 ttttacttgc tgcggatgag aacgacgaag aacgatgtgc atagcctgaa gtgtacataa
    16681 tcaatccagc ccctgtgagt tactgtatga atatacagta catccagtga caacaaagat
    16741 caaccctatt ttcggaaaga gcctcgcaaa ttttttcgtt ggtgacggga aaacataaat
    16801 taatcttgcc ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga
    16861 atcattcttt tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg
    16921 atctataaat cacaagaata cctggcaatg ttgggcacaa ttgaataccg atcattgttt
    16981 ggcagttaca gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg
    17041 tatcttcggg cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg
    17101 acatataaaa agtgtggccg atccgttacc cttaattact atcgggttga tgaaagtcta
    17161 tggcgaaatc aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa
    17221 gagaaaagca cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat
    17281 ctggaagcga ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg
    17341 gcaaaaatgt gttggttgcg actgcggcag caaaacagtc tggtgacaga aaagattctg
    17401 tttatgcttg aaggtgccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc
    17461 caggagcttg cagaatgggc tgactcactt acgccgaaac aggagtttcc tgcggaactt
    17521 gagtaatctg gcgttgcaga cgaccaatct caggcagtaa tgcaatcaat aatccaactt
    17581 gttgtacgac cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt
    17641 tcaggctagc taatgcctca ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt
    17701 catcaacata gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct
    17761 cccggtgagc accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc
    17821 gaaacgcggc ttcgcgaatt tgcggggtaa cgttcggctc gctggacata tttgatacca
    17881 ccgacgccag ctcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac
    17941 ggttatcacg cccctgatgg tattgctcca gtatggcatc gagataccga cagttggcct
    18001 ctgtggcgcg ttcgagcatg cgcggcaaat tgcgaaactg ccagtcaggc cagatgtagc
    18061 tcactgccgc ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta
    18121 cttcaaaacc ttcacccagt aagttaaaac acagtagcac caaaagtgtg atgaacatcg
    18181 ttgcatgagc gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca
    18241 gcaccagctg cccttccagt gatggcacaa accacagcac aggaatgcca atggcgatac
    18301 ctaccagcgt accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct
    18361 ggcagacaaa caaacttgtc agcaagatcc aaaacccgtg atgcattccg gttatttgaa
    18421 tgatggcgta gccgaagcac aacaccagcg acattcttac cgcatgacgg aagagggcgg
    18481 attccggcgt gaagtgacgg ctaagacgca gccagatatc actcaacccg tgcgggctgt
    18541 catcagcgag ctcattttcg tcattattat ggggtagtgc ctgggcctgt tctgattcaa
    18601 ttgttgccag ttgggcatca atggcgcgta aattgttcag caaaaatccc agtgttttga
    18661 gtaaatcggc gggtgcgccg ttatcgcgca tccgctccag cgcagcatca atatgcgtaa
    18721 aagcgcgctc aaaatgcgga tcatgttgat aaggctgacg caacaaaata cagcgtgaca
    18781 gttgctggca cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc
    18841 tgtggcgaaa atgttcacgc aatgtttgat actgaatatg agaagagctg gcacgctcgt
    18901 gaatatcctg tgcgacaaag taataatgca gcgtgcgacg cgttccccgt tgaccacgat
    18961 cgccacgtaa gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagcagac
    19021 cgttggcgag agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa
    19081 acatgcgcga cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt
    19141 cctgcagcgg gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga
    19201 cggcaccggc cagcagatac atcggctgct gataccagtg ctcatacagt gatgttccca
    19261 acatagtgta aatggcgatc agcaatgcac cgaaggcaat tgttgcatag cgttgaccca
    19321 gaccgccgag caaaatgaag ccgctggtag agagcgttaa gccaatcgca aatagccagg
    19381 gccagggaaa cagcaattct actgaggccg aggcgataaa aaagcagaac agcgtaatga
    19441 tgaggttacg caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca
    19501 tccccagcgt tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg
    19561 ttccacaaag cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc
    19621 gtttgagcaa aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg
    19681 cattggcttc acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc
    19741 atagtgcaat agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgattgaaa
    19801 tacattgtgc gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca
    19861 gccagaaaat attcaatacc gtaccgccag tattcagcag cacatttttg ccagccgggt
    19921 tcagttcatc gacatgaata gcttcattgc cataaggcac cagagacagt ttagtgatct
    19981 cccagcagga tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca
    20041 acagccagcc cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg
    20101 tacgcataaa acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt
    20161 ttgggctgga gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc
    20221 tacagagtca tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac
    20281 gatcgggcgg tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt
    20341 attcctttca atcaattact ggcgattcac tgtaagcgtg gtctggtatg gggcgagctg
    20401 gaatttgtac tgccggacga aaaagtggtg cgtctgcacg gcaccgaatg gggcgagacg
    20461 cagcgttttt accatcatct tgatgctcac tggcggcggt ggagtggcga gatgagcgaa
    20521 attgcgtctg gtgttttacg ccagcaactg gatttgattg ccacgcgcac tggagaaaat
    20581 aaatggctga cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg
    20641 gcgttgccgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    20701 tggcgtaaat gtcaggcctg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    20761 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    20821 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    20881 gcaggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggca
    20941 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    21001 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    21061 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    21121 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    21181 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    21241 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    21301 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    21361 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    21421 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    21481 catcaggcga ttgtgattct ggagaaaggt cgctttatca gcccgtggaa gcatattctg
    21541 gttgatgaat ttcaggatat ctcgccgcag cgggcagcgt tgttagcggc attacgcaag
    21601 caaaacagtc agacgacgtt gttcgctgtt ggtgatgact ggcaggcgat ttaccgattc
    21661 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgaacgt
    21721 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtaaggtggc aaaccgtttt
    21781 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    21841 aaagccgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    21901 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    21961 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    22021 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga gggaagtgat
    22081 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    22141 gatttcccgg acgctgaaga acggcggtta atgtacgtgg cgctgacccg ggcacgccat
    22201 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgttgaaat actgaaaaat
    22261 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    22321 attacttcag acggtccgcg agataacgct gataatcggg gatcagaata tcgaccgcgt
    22381 cgttgaaatg cggcgactgg attatgaagt ctgccgttgc cacgttggcg gcgaccggaa
    22441 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    22501 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    22561 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    22621 gggaaattaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    22681 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    22741 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagtcaac
    22801 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    22861 acaggcacaa aaatctcagg aagatggttg tttttccgcc cactgcagga aagtatttcg
    22921 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    22981 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    23041 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    23101 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    23161 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    23221 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    23281 aaaactgacc actagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    23341 cgtcttctca acgcgaaaca ctaactgatg cggtccatta tccagttcaa tgctgtccgc
    23401 accgcgcagc agagagctgg aaaccttctt gccgtcgagt accagaagat cgacatcggt
    23461 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    23521 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    23581 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    23641 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    23701 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    23761 gctggtgggg gcaagcgaca aacccgatcg tcccagctat cgcgtgatga aatatctact
    23821 cgaccagggg tatcatgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    23881 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    23941 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    24001 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    24061 gttgaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gcctggccaa
    24121 ataaaaaatc cccggaaggc aaaaaccttc cggggattcg ttcagggatc agttacgcag
    24181 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    24241 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    24301 taggccgtta tcgtcctcca tcactacgtg ataccacgga gcggcgcgaa gctcgtcatt
    24361 taccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    24421 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    24481 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    24541 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccggat aggtagcgat
    24601 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac ggccggcatc
    24661 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    24721 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatatcttt
    24781 atagccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    24841 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    24901 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    24961 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    25021 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    25081 gtagcggcgg gtagccaggc ggctgtctcg ctggtccagg tagtagcccg ttttgtgtcc
    25141 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
    25201 caactcgccg gtgacgaggc ccagggtcag ctccatccct tcttttttac gtaccgcaac
    25261 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taactaatgc
    25321 cgcgcgctga tattctgccc cagcactcag cagttgcagc accagaaaat taccgaaacg
    25381 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    25441 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    25501 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    25561 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    25621 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    25681 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    25741 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    25801 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctgggttta
    25861 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    25921 tttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    25981 agaagggcag gtggaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    26041 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    26101 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    26161 acctttcgga aacagtcggt ataaatagcg gctgttacct ttttcttcgc caaatttatt
    26221 cgccatcgct tttaccagca tacgaatcgc cggagaagta ttgaattcca gatagaaatc
    26281 acgcacaaaa cgcaccactt cccagtgttc cggtgacagc gaaatccctt cgttctctgc
    26341 aatcaccact gccagcggct cactccactg gctgctttct ttgagatagc cttcggtatc
    26401 cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    26461 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    26521 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    26581 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaacgatgtt
    26641 gctggtttca aacaaaatag cgccagagga gatcaaaatg aagaccgcgc tgatcgccag
    26701 atgcagagca ggcagctgca ggaagatatt agcaaccata ccaatcagca ccaccacaat
    26761 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    26821 agagcagcag aagaacacta acgccgttcc gcccagagcc atagcgatta cgtcacccat
    26881 tccggcagac agataggtgt tcagaatagg tccgaggata taacccagaa aaccggtaaa
    26941 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    27001 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    27061 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    27121 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    27181 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    27241 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    27301 tatcaggtca ttaagcaaat ataacgccct gagaatttcg acaggcaaaa gaaaaagggg
    27361 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    27421 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    27481 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    27541 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    27601 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccac cgcccggcaa
    27661 aaagaaaaat aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt
    27721 gtgcaaaagt ttcactacgc tttattaaca atactttctg gcgacgtgcg ccagtgcaga
    27781 aggatgagct ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac
    27841 gtcgcttgcc gaccataagc gcccggtgtc ctgccggtgt cgcaaggaga agagacgtgc
    27901 gatatgaata acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc
    27961 agctttctca aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca
    28021 ccaaagattg cctgggcgct ggagaacaaa ccccgcattc cggtggtatg gatccacggt
    28081 ctggaatgca cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac
    28141 gtcatccttt ccctgatttc cctcgattat gacgatactt tgatggctgc cgccggaacc
    28201 caggcggaag aagtcttcga agacatcatc acgcaataca atggcaaata tatcctcgca
    28261 gtagaaggta atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg
    28321 tttattgaga aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc
    28381 tgcgcgtcct ggggctgcgt gcaggccgcg cggcccaatc cgacgcaggc aacgcctatc
    28441 gacaaagtca tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat
    28501 gtgatgagcg ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga
    28561 atgggccgtc cgctgatgtt ctatggtcag cgaatccacg ataaatgcta tcgccgcgcc
    28621 cacttcgacg ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttac
    28681 tgcctgtaca aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc
    28741 tggaatgatg gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa
    28801 aatggtttct gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt
    28861 actcattcca ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcggctgtt
    28921 ggtgtgcacg cagtcgccag cgccgttgac cagcgcagac gtcataacca gcaacctaca
    28981 gaaaccgaac atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc
    29041 agggatacac catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg
    29101 aaggccacat gcgctgcgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct
    29161 cctgcggcac catgtttcgc gggctggaga tcatcctaca agggcgcgac ccgcgcgatg
    29221 cgtgggcgtt cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg
    29281 tttacgccat cgaagatgct atcggtatta aagtgccgga caacgccaat atcatccgca
    29341 acattatgct ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg
    29401 ggatggactg gatcgatgtg ttagatgcgc tgaaagccga cccgcggaaa acctccgaac
    29461 tggcgcaaag tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa
    29521 accgcctgaa aaaatttgtt gaaggcgggc agttggggat cttccgcaat ggctactggg
    29581 ggcacccgca gtacaaactg ccgccagaag ctaacctgat gggctttgcc cactatctcg
    29641 aagctctcga tttccagcgt gaaattgtca aaatccacgc ggtctttggc ggtaaaaacc
    29701 cgcatccaaa ctggattgtc ggcgggatgc cttgcgccat caacattgac gaaagcggcg
    29761 cggtcggggc agtcaatatg gaacgcctga acctggtgca gtcaattatc acccgcacgg
    29821 cggacttcat taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac
    29881 cgtggagcga aatcggcact ggtctttctg ataaatgcgt tctcagctac ggcgcattcc
    29941 cggatattgc caacgacttt ggcgagaaaa gtctgctgat gcctggcggc gcggtgatta
    30001 acggcgactt caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt
    30061 ttgtcgacca cgcctggtat cgatatccca acgatcaggt cgggcgtcat ccgttcgatg
    30121 gcatcaccga cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc
    30181 agctgaatga acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga
    30241 tggaagtggg gccgctggcg cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg
    30301 ttgagtcggt cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa
    30361 cgttaggccg cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt
    30421 atttcttcga caagctgatg atcaacctga aaaacggcaa tctcgccact gcttccacgg
    30481 aaaaatggga acctgcaacc tggccgacag agtgccgtgg tgtcggtttt accgaagcgc
    30541 cgcgcggggc gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt
    30601 gcgtggtgcc gaccacctgg aacgccagcc cgcgcgatcc caaagggcag attggcgctt
    30661 atgaagcggc gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatcctgc
    30721 gtactctgca cagctttgac ccgtgcctcg cctgttcaac acacgtgctg ggcgacgacg
    30781 gtagcgagct gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga
    30841 aaagcgacaa cgttgtcagc cactatgtct ttgaagcgcc agtccgcatc tggcactggt
    30901 tgacggtgtt atgcatggcg gtgttgatgg tcaccggata ctttatcggc aagccgctac
    30961 cttccgtcag cggcgaggcg acgtatctgt tctatatggg ctacatcagg ttaattcact
    31021 tcagcgccgg gatggttttt accgtggttt tgctgatgcg gatctactgg gcttttgttg
    31081 gcaatcgata ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg
    31141 gcgtgtggta tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag
    31201 gccataatcc catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga
    31261 tcatcactgg ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc
    31321 gttatgtggt ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc
    31381 atcggctggg gatgtggctg attggcgcgt ttgtgatcgg tcatgtctac atggcgctgc
    31441 gtgaagacat catgtccgac ggcacggtga tctccaccat ggtcaacggc taccgtagcc
    31501 acaaatttgg caaaataagt aacaaggagt gttcatgagc gagcaacgcg tggtggtcat
    31561 ggggctgggc aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct
    31621 gtatgcccat taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact
    31681 gaacttgctg gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta
    31741 cgggctggaa cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cttatctcag
    31801 cgcgaagaaa atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat
    31861 ccgcggacat ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga
    31921 ctacggcggc agcctgagcg aactggcacg ggagcaactg ctcgctgcgg aacaatcggc
    31981 gctggcgcag ttggctgcgt ggggaagtgt gccgcaactg gctaatgaat cgcgttgtct
    32041 caattatgac tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat
    32101 gacacaggag gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg
    32161 ctggcgcgcg gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg
    32221 ccagacggcg tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat
    32281 ccggtaatga ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt
    32341 gctgaccttg agcagagcga agccatcggc gatcgctttg gcgtctttcg ctttcctacc
    32401 actttagtgt ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg
    32461 gaactgataa acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg
    32521 aaactttttt ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga
    32581 atccactgcc gatcacctgt caggtgaatg atgaaccgag tatggcggcc ctggagcaat
    32641 gtgctcacag cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac
    32701 gccagccgcc gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc
    32761 gtcactttat caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg
    32821 acgacagtaa aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac
    32881 gtcgcggcga aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt
    32941 ggcaggcggc aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg
    33001 gcctgatgaa tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg
    33061 cgcagccgca cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc
    33121 tctcacgtct ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct
    33181 atatcaacgc cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag
    33241 gcccgttact ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag
    33301 aagatttggt cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg
    33361 caccgacgta aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa
    33421 tacctctggt cgtagagttt caggataaag agggagatct aacattatcg ggttattttt
    33481 ctctcttcgc ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt
    33541 gctctgaccg cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg
    33601 ctggctatca tggaaaccat ttacgtggtc accggcaaaa caatctaccg cgatatgacg
    33661 cgcttctggg gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc
    33721 atggagtttc agtttggtac taactgggca ttctattcca actatgtggg cgatattttt
    33781 ggcgcaccgc tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg
    33841 cttttcttct tcggctggca gcggctgaat aaataccagc acctgctggt gacgtggctg
    33901 gtggcgttcg gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa
    33961 tacccgaccg gtgcgcattt tgatatcgac accctacgta tggagatgac cagcttcagc
    34021 gagctggtct ttaacccggt cagccaggtg aaatttgtgc acaccgtaat ggcgggctac
    34081 gtgaccgggg ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag
    34141 cgcgatgttg cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc
    34201 ggcacgctac aactcgggga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa
    34261 ctggcggcga tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt
    34321 gcctggccgg aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgtta
    34381 gggatcctcg ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct
    34441 gaaacctacc cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatcg
    34501 caaggcaatc gtgagccgca tgtgttgcag gcattccggg aactggaagg tgacctgggc
    34561 tacggcatgt tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac
    34621 caggcggcga tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc
    34681 atggtgggct gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg
    34741 cgtggcaaaa tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg
    34801 ccgtggattg cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg
    34861 atacaggaca tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct
    34921 ttctcactga tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg
    34981 atgcagaaat atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa
    35041 caggggtaaa ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc
    35101 tgattggcgt gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct
    35161 gtctgctgcc gctggtggcg cgtaatgatg atgaacgccg gatagtgata aacagcgttg
    35221 gtgcacactg ggaaggcaac caggtctggt tgatcctcgc tggtggggca ttatttgccg
    35281 cctggcccag agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc
    35341 tgtgctcact gttcttccgc ccgctggcct ttgattatcg cggaaaaatc gccgatgcac
    35401 gctggcgtaa aatgtgggac gccggtctgg tcatcggcag tctggtgccg ccggtagtct
    35461 tcggtatcgc cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac
    35521 gcgtggagta tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgcggat
    35581 tgctcagcct tgggatggta attttgcaag gtggcgtctg gttacaactg aaaactgttg
    35641 gtgtgattca tctgagttca cagctggcca ccaaacgcgc tgcactgttg gtgatgctgt
    35701 gctttttgct ggcgggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc
    35761 aggatgctaa cggtccttcc aatccgttaa tgaaactggt ggcagtgcta cctggtgcct
    35821 ggatgaataa tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct
    35881 gcccattgct gacggtgatg gcgatttatc gtggtcgccc aggttgggga tttttaatgg
    35941 cgtcattgat gcaatttggc gtgattttca cggcaggcat cacgctgttc cccttcgtca
    36001 tgccgtcaag cgtgagtccg atctccagcc tgacgttgtg ggacagcact tccagccagc
    36061 tgacgctgag cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca
    36121 ctctctggag ctactacaaa atgtgggggc gcatgacaac agaaactctc cgccgtaacg
    36181 aaaacgagtt gtactaagga gcaggaacaa tgtggtattt actttggttc gtcggcattt
    36241 tgttgatgtg ttcgctctcc acccttgtgt tggtatggct ggacccgcgt ctgaaaagtt
    36301 aacgaacgta ggcctgatgc ggcgcattag catcgcatca ggcaatcaat aatgtcagat
    36361 atgaaaagcg gaaacatatc gatgaaagcg atcttaatcc catttttatc tcttctgatt
    36421 ccgttaacct cgcaatctgc attcgctcag agtgagccgg agctgaagct ggaaagtgtg
    36481 gtgattgtca gtcgtcatgg tgtgcgtgct ccaaccaagg ccacgcaact gatgcaggat
    36541 gtcaccccag acgcatggcc aacctggccg gtaaaactgg gttggctgac accgcgcggt
    36601 ggtgagctaa tcgcctattt cggacattac caacgccagc gtctggtggc cgacggattg
    36661 ctggcaaaaa agggctgtcc gcagcctggt caggtcgcga ttattgctga tgtcgacgag
    36721 cgtacccgta aaacaggcga agccttcgcc gccgggctgg cacctgactg tgcaataacc
    36781 gtacataccc aggcagatac gtccagtccc gatccgttat ttaatcctct aaaaactggc
    36841 gtttgccaac tggataatgt gaacgtgact gacgcgatcc tcagcagggc aggagggtca
    36901 attgctgact ttaccgggca tcggcaaacg gcgtttcgcg aactggaacg ggtgcttaat
    36961 tttccgcaat caaacttgtg ccttaaacgt gagaaacagg acgaaagctg ttcattaacg
    37021 caggcattac catcggaact caaggtgagc gccgacaatg tctcattaac cggtgcggta
    37081 agcctcgcat caatgctgac ggagatattt ctcctgcaac aagcacaggg aatgccagag
    37141 ccggggtggg gaaggatcac cgattcacac cagtggaaca ccttgctaag tttgcataac
    37201 gcgcaatttt atttgctaca acgcacgcca gaggttgccc gcagccgcgc caccccgtta
    37261 ttggatttga tcatggcagc gttgacgccc catccaccgc aaaaacaggc gtatggtgtg
    37321 acattaccca cttcagtact gtttattgcc ggacacgata ctaatctggc aaatctcggc
    37381 ggcgcactgg agctcaactg gacgcttccc ggtcagccgg ataacacgcc gccaggtggt
    37441 gaactggtgt ttgaacgctg gcgtcggcta agcgataaca gccagtggat tcaggtttcg
    37501 ctggtcttcc agactttaca gcagatgcgt gataaaacgc cgctgtcatt aaatacgccg
    37561 cccggagagg tgaaactgac cctggcagga tgtgaagagc gaaatgcgca gggcatgtgt
    37621 tcgttggcag gttttacgca aatcgtgaat gaagcacgca taccggcgtg cagtttgtaa
    37681 tgcataaaaa agagcattca gttacctgaa tgctctgagg ctgatgacaa acgaagaact
    37741 gtctaatgcg tagaccggaa aaggcgttca cgccgcatcc ggccactttc agttttactc
    37801 tttctcggag taactataac cgtaatagtt atagccgtaa ctgtaagcgg tgctggcgcg
    37861 tttaatcaca ccattgagga tagcgccttt aatattgacg cctgcctgtt ccagacgctg
    37921 cattgacaaa ctcacctctt tggcggtgtt caagccaaaa cgcgcaacca gcaggctggt
    37981 gccaacagaa cgccccacga ccgcggcatc actcaccgcc agcatcggcg gcgtatcgac
    38041 aatcaccaga tcgtaatggt cgttcgccca ttccagtaat tgacgcatcc gatcgcgcat
    38101 cagcagttca gacgggttag gtggcacctg accgcgagta atcacatcaa agcctccttt
    38161 gccaaaatgc tggatcactt tgttgagctc atctttacct gccagatatt ccgacaagcc
    38221 atgttcatta ctcacggtaa acaggttatg cgaataacca cggcgtaagt cggcatcaat
    38281 aaataacact ttttgatcgg actgggcgat caccgctgcc agagttgaac tgacaaacgt
    38341 tttaccactg tctggcgtcg caccggtgat catcagaatg ttattctccg tctccatcat
    38401 agcgaagtgc agactggttc gtagcgcacg tacggcttcc acagcagaat ccgccgggtt
    38461 atccaccgcc aggaagggga tatttttagt acgatggcgc tgctgattag aaaataaatt
    38521 tttcttacgc agacgggtac gtttatccag ccactcggac attgggatag tggcataaac
    38581 gctgatgccg tgctcttcca gttgttccgg ggcttctaca ccacgacgca acatcgcacg
    38641 cgccagcacg gcacccaccg aaataaacag gccaagaata aaaccaagca ccacgttcaa
    38701 cgcttttttc ggtttcactg gctgcggctg agtgactgct gggtcgataa tccgcacgtt
    38761 accaatggca ctggatttcg aaatactcaa ctcctgctgg cggttaagta attgcagata
    38821 taccgcacgg cccgcttcta cgtcacggct taaacgtaac acttcctgtt gagtggaagg
    38881 cattgccgat acccgcttat tcaggcgttt gcgttcttgc tccagcgtct ggcgtttttc
    38941 cagcagcgca cgataagttg ggtgatcttt cttatacagc tgggagatct ctgcctcgcg
    39001 gaaagtcagc tcattgagtt gattatcaac gttcacaatc tgctcaagaa cggctttggc
    39061 ttctaggtta aggtcaaccg aatcgcgctg ctggcgataa acgttgagtt tttcttccgc
    39121 ctggtccagc tcgctgcgca cttcaggcaa ctggcgctgt aagaattcaa ggctttgtga
    39181 atcctgcgcc gcctggcgag cgatattctg ttgcaaatag ttgttagcga tgctgttcag
    39241 aatacgagta atcaactggg gatcatcacc agtcatggta agttccagca tcccgctttc
    39301 tttactgcgt tcgctaacgg taaaggtttc ctgcaatgcg ttaatcgctt ccagttcggt
    39361 acgctggctc aggacaaact gtgttcctgg tttggcctta atgtccgcga tagtcagcgc
    39421 aacgccatct ttttccagac gctggccgac cataccattg acggtgaact cttcaccttc
    39481 cagtgtatag tggccgtttt ccccaaccgt gagtgtcagt tgctgatcct gaccattcag
    39541 ttgtggaata tgcatccagc tgatcgccag ctcacctggt ttttctttgg ttaatctcgc
    39601 ccagccgcga cccacaatcg gaaaatactt ctgctcaact atgtcgtgca gattcagttc
    39661 agcaatggtt ttaccgagaa tcatgcgcga ttgcagcagt tggatctccg gtgcagactc
    39721 gggcgatgag ttagggatca tatcgctcag gccgctgaga atggcgttgc cctgtttttg
    39781 ctcaacctgg accagagtat ctgcctgata aattggtgtg cttaacagcg agtaagcgac
    39841 agcgatcagc gtgaataacg cggttacgct gataataaac ttacggtgat cccataactc
    39901 gccgaccaga cgaagcagat cgatctcatt ttcctgagtg ctgcctggtg gcgtattcat
    39961 atttttagtt gtcatcgtat tttataaact caaattaccg gctgaggcgc ttcgcccatt
    40021 cctgactggc gcgctccaac ataccgtaga catgttcaaa tgcgtcctga cttttacgat
    40081 agggatccgg gatctctttc tgttccagcc attgcccaaa cagcattgtc ttcccgcgga
    40141 cctcgggagc gattgcggta acctgagcaa tatgctctga ctccattgcc agaatcagat
    40201 cgtaatttcg tgccatctca gcagtgagct tacgtccggc atgtccttca agagatacgc
    40261 catgattagc ggcaacatct gctgctgtcg catctgcagg gtgttttacc agaccatgaa
    40321 cacctgccga tttgactttt acgcctggca gacgtttacg cagcaagcgc tcgccaatgg
    40381 gcgaacggca aatgttgccg gtacaaacca ccaggattga gttaaatttt agttgggcca
    40441 tgtttgaatc caccgtgaag tctctgtcag gtcatgcacc ccggaaatcg tcggtaccag
    40501 cagagaaatc acacggttcc aacgagccag cggcgcggta gtgacataaa caatgtcgta
    40561 aggttcaagc tggaattccg ttccgagtat cattgccgat gcgtccttcg cattcagctg
    40621 ataaatattg gcgattttgc cattctgctt gttttgcgtt gcacgaataa caaaaatccc
    40681 cgtcgcatca gcgacatcct gattcatacc ctcagcgtta cccagcgctt ccgccagggt
    40741 cataccgctg cggtccattt tcagcgtgct ctgtttaccc acttcaccca tcacgaagac
    40801 tttcagatca tcgttgcgcg gaataaataa aatatcgccc gggtgcagca gtttgttctg
    40861 gcgcaagtcg ccacgctgca tcagcgcata gaggttcact ttggtcttta cgccattctg
    40921 ggtgagcacc acgttgcgcc agtctgcatc tgcggtcaga ccgcccgcgg cgttaatcgc
    40981 atccatgatg gtcagaggaa tattggtaat cggctgctga cccgatttgc tgacttcgcc
    41041 ggtgacgtac gctttttgcg atcggaacgc cgcgacgctg acatcgactt gcgggctttc
    41101 aatgactgaa tcgagacgcg ctgtaatctc gttgcgtacc tgggtaagtg ttttcccggc
    41161 cactttcagg cgaccaatat aggggtagaa aatcgcacca tcggcgttaa cccagttgcc
    41221 ggtatcactg gcgctacggt attgccctgc gggggtggtt agttccgggt gatcccagac
    41281 cgtaaccatc aacacatcac caatcccgat acgatattca tagtttgcga tttgctgctc
    41341 cagctccggg ttagcctgcg acataatcgg tttggcgcgc aactgatcaa tcaatcctgg
    41401 cgtgacggga tagatattga ccattttgtc caggtcatac tgattgtcag gtagttcaat
    41461 gacatccttg ttcctggtcg ataaattctg accaggaaca agggtacatg ctgtcaaaga
    41521 cagaacagcc aatgtcagta ccgaaaactt aaaaatgttc tttttcattg gtttatattt
    41581 atcagtcaat gtaatgttat gaattacgtc aggttattgt ccaaccggta cttctcgctc
    41641 tgccgtcatt ggatagagct ggtatttgcg tgacagcatt tgtccaccat cacgcgtcaa
    41701 tggtgtccac gaaaccaccg cgcggttgcg gttaggtcca atggtcatca agtcgaatgg
    41761 aatcgagata taaaaacctt tactaaagcc gccttcgccg tagtcatctt tagatacgtt
    41821 actgattgcc gcccataccc ctaccgccac gccgctgtca aagcgtttgg cgacgtcgat
    41881 cgttgctcct ttatcttttg ccagatattg cccaacgcta agtttcatca gtacgccatt
    41941 gagcgtcggc gggttccagt aagccgtcac gaaaccagtt ggcgtggaat aatcggtgaa
    42001 gcgcatcatg ttgtcccagt cacgttgctt cacgtagtta acgtccacac ccagcgccca
    42061 gctggcatct agcgggcgat aaagcagctc ggaaccgaca ccggcgtaca tcgtttccag
    42121 ataaccgcca tacacctggc catagaaacc attgcctaag tcggcaaagt agttcgcctg
    42181 caagttgttg agataaacgt cattgcgaac gtaatcacgg atatgcgtgc gcacgcgggg
    42241 cagggtagag tccgcgggca acagcgaaga cttaaacttg tcgtagttgt tgtaaatatt
    42301 ggtgaaaata ccgccatcaa gcagcaggtg gtcggtaaac cagtagcggg cactggacat
    42361 cagccccagc tggaacatat agaaatcttc cgggccgccc agcgactgtg aaagtgttgg
    42421 gttgaaagag tagctaaagc gatcttcacg aatacggtag ccccgaccaa aggcagaaag
    42481 atcttctgct tcaacacgtt gttgttgaag ttgctctgat tgaccaggcg ctgtaccagc
    42541 cagctgtttg cgcaggctgg ctacgtcggt ttcggtagtc accatcgcca tatgctcgcg
    42601 cttttgcgtc acgctaatct tctcaacgcc ttgcggcagg ttattcacca gaatccggtt
    42661 ggcacgatcg accgcttcgc gagaatcacg gtatttgtat tgttgaccag acatatacag
    42721 cgtcttatcg cgcagctgaa tttccggtgc ttcaaaacct gcgttgtact tcagcgcggt
    42781 aagttggttc gctactgtgg tgtactgcaa tccttcagat tcaggcgcag gttgatatgc
    42841 cggttttggc gtatcgcgca gggcagggcg cagatcgttg aaattggtcc gtaacgtgaa
    42901 gccaaacatc aacgtgttac cgcgttcata actcaggttg agatctgccc agctggcagc
    42961 gcgataaact gcgccgacgt tgaaatggct tgcctgaggc agtttgccag cgaaatcatt
    43021 ctggtaattg ttgccatcgt attcgagttt cagacgcagg ggattccacg gcgtttgata
    43081 ctcaatgccg ccaaagatgg aagccgggcc acgaaagata tcgctaaagc tgatatcgcc
    43141 cgcatcgtga gactctgcgc gatgacaata tttatcgttc acccggcaaa acgggttggt
    43201 aatattgccc gcattaccgg cgtagcccca tgccatcccg agggtgaaat caaatggccc
    43261 cgcttgcttg ctggccacca gatactcacc gtcaaacaga cccgtaccag caatatcacg
    43321 tttaccaaac gccacttgcg gtagccagta accttcttcc cacaggcgaa gtttaaaatc
    43381 gaatgatttg tctttgtatg actgatcgcc gctgaaatct tcccactggc tatatttgcg
    43441 tgtgcgcaca tccgtataac gaatggtgcc ttccagccat gggaacagcg ccacggaggt
    43501 ggagtagaac cggtattgat cgttatcccg gtagttgacg ctgaattcac cttccggtgc
    43561 gatgcgcgca tttggcatct gcaacaatcc tgtgccgcca aagtctgatt gcgacgaacc
    43621 cagcggatcc gggtaggtta atacttcagc atgacaggca ctggagacgg caatggccag
    43681 gcagcttaaa agataagaat tcttcttcat cagtcaggaa cacgttgcgt taaaacagac
    43741 acaatctggt catttaaatc agcatatttt tcgggcagta catgcgcaga aaagcccaac
    43801 caaagctggc tgccaggcgg tggttccaca tgtcttttat tccacagggc aacaggggcg
    43861 acgacagtct caccctcagg cgtaatcacc ataacgttat tcttgtccgc gcctgcaagg
    43921 cgggggtgat cttgcagata atcggtaacg ctacggcctg cctgccaggg caattgcccg
    43981 gccccagaaa ccgcacccag cagagtaata gtcaccggac gctgtacggt atagagcgta
    44041 tagtccccga ccaacggggg attgctgttc tcatccactc gaacaaaatc agggtccagt
    44101 ttgaccggta agcgaccagt gatattgaga ttgagtaact gctggcgcac agatttaatc
    44161 gttgccgcca catcgtcgtc agcttctgct tcccaggaag caagctgtgc catgacatgt
    44221 tggtagtctt taagcgcttt agctttcgcc gcagagtcgg tcagcaaggc accaggccac
    44281 cagaggcgat cgcgcagttg cggttgtgtc accaactgga caacattttc catggggcca
    44341 acggaaagtg tctgttgttc gccaggcaga taaatagtca ccgttccttg cgcaaaggca
    44401 tggggtgtca ttacgtaaag tacgctggca atgaaatacg actgtaattt attcatggtg
    44461 cgggtttaag aaacgtcatt tctacgggaa taacccctgc gcctaacatt tgccgacttt
    44521 gacgaacctg gccagtagcc gaatcgaccc agaatgtgtt ttgccactgt ttttctggcc
    44581 gcgtggaagt cacctcttcc tgccagacat tacacagcac attgcgaccc gcaatattca
    44641 gcgtctctaa tccagcaaag gaaaaagtag aactcagggt cgcagagcga aaatggttgt
    44701 cttcactcca gagaatatcg cgcgtccagc ggctgccatc ttttattgcc agcgcgttac
    44761 gcagagggtc ctgcccggaa tttgtcactt ccagcagatt attattaagt ttgacggttt
    44821 ttaaaagacg tccattgtgg gtaaccagca tggcgttatc ctgggataac catttgcttt
    44881 gttcttgttc tatatatccc agtaccacaa agattcgttg cccttcatta aggcgtaaat
    44941 acatcgtgga ataaggcaac gcctggatct gctgatccgc tacggtgata tcctgattat
    45001 cgaaaaggct ggcgcgaaat gtatcgacca tactttgctg gctgtgcgta catcccgcaa
    45061 gaaatagtgc gaaaatcgat aaaataagag ggcgcacctt taatccttga aaggttaatt
    45121 aaaaaaatgc aaatctttaa agcccggtac gaatatcgca accgggtatc atttaacttt
    45181 aatttcgacg cgttaaagca gtattagcgt gtagtggacg tggtggttga ggtgttatga
    45241 gaaccgccat cgccactgtc atttgctgca acaactgcca acgtcgccac accaacagcc
    45301 gttgccacac cgacaactgt cgccgtgccg acagcgccag cagccacgcc accagtggta
    45361 gtagcagtcg tggttgcgcc ggtagtggtc gttgtggtgc cagtagtacc cgtcgttgcc
    45421 tcggtcccgt ttgttgcttc aggggcagca aacgcagctg gcgtcgtcaa catgaaggcc
    45481 ataaggattg cagaaagctt gtgtttcata acttttcctt tattcatcgc atggacaata
    45541 cgtacgcttg agatcaggat tgtctacttt gagtataaca tccttgtgaa atctcccttt
    45601 cctcataccg cttgcactat atactgcggg taggaaaggc gcgcagaggg aaatataaga
    45661 ttgttaacta tccccctctg gaaatacgac ttgtgagcat aagataaatc ttagcaaaat
    45721 acaaattaaa gataaggaaa ttgttacgaa agctattaat tattgctggt aatatagttt
    45781 caagtggtac tataactggc tgttaaatgg catttataac tattaggtgc agagatattc
    45841 gcttaaatgg attagtttct ttgtggaaaa gtaactgatg ttataatata tgatgttgac
    45901 tattgcacta ttccaataaa accagtcagc tttaaacaag cagcgtcata ttaagagaga
    45961 taaacatttg ccgctgttgg tcctcgcagg ccatttacgc ggcaaaattc cacacgtaat
    46021 cctggtataa gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac ctgaacttct
    46081 ttacgaccgt cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa ggttttgaca
    46141 attcctgtca ttttacggga caaacaaatt ccttactgaa aatactgcgc tgcactatac
    46201 ggggttaata aaataaagcc agcgatattt aagaccgccg gacggctaaa ataaaatttg
    46261 cttaatctca attatcatgc gttaatagct gcgtcggttt gaaagacaga cagcatacaa
    46321 agtagtttac taaagcagtt ctcattatca ggcattatcc ccttcttttg agtctctctc
    46381 ctgaacacta agtagtttct gtattaaagc cctgtttgcc gaaaggccca aaatgaagga
    46441 agtaaaatat gtctaataaa atgactggtt tagtaaaatg gtttaacgca gataaaggtt
    46501 ttggctttat cactcctgat gatggcagca aagacgtttt cgtccatttc actgccatcc
    46561 agagcaatga attccgcacg ctgaacgaaa atcagaaagt tgaattttct attgagcagg
    46621 ggcaacgtgg ccccgcggca gcgaacgttg ttacgctcta aggttgccgt tattactcaa
    46681 catctccatt tccgctgtcc atgttgtcat ggttcacagt accgcacatc ggcattcgat
    46741 gtgacggagc gaaatccttt gggcgctaag tgtatttttt gtaaatcgac gatgatca
//
LOCUS       DAJTYD010000008        45172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTYD010000008 DAJTYD010000000
VERSION     DAJTYD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.008"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1718
                     /gene="fhuE"
                     /locus_tag="OO585_000449"
     CDS             <1..1718
                     /gene="fhuE"
                     /locus_tag="OO585_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCR6628145.1"
                     /translation="EVVRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWN
                     KERYVADLQSPLTEDGKIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLS
                     AGYEYQRIDVNSPTWGGLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRF
                     ADTWQATLNATHSEVEFDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGK
                     RKVDALDLFADGSYELFGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNF
                     PQTDWSPQSLAQDDTTHMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHT
                     TPYAGLVFDINDNWSTYASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRL
                     TTTLAIFRIEQDNVAQSTGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTF
                     GATRYIAEDNEGNAVNPNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPY
                     GTFRAEQGSYALVDLFTRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSIT
                     GTYQF"
     gene            complement(1778..3211)
                     /gene="ptsG"
                     /locus_tag="OO585_000450"
     CDS             complement(1778..3211)
                     /gene="ptsG"
                     /locus_tag="OO585_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6628146.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3506..4303)
                     /locus_tag="OO585_000451"
     CDS             complement(3506..4303)
                     /locus_tag="OO585_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6628147.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4314..5318)
                     /gene="holB"
                     /locus_tag="OO585_000452"
     CDS             complement(4314..5318)
                     /gene="holB"
                     /locus_tag="OO585_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6628148.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5315..5956)
                     /locus_tag="OO585_000453"
     CDS             complement(5315..5956)
                     /locus_tag="OO585_000453"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6628149.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(5946..6968)
                     /gene="yceG"
                     /locus_tag="OO585_000454"
     CDS             complement(5946..6968)
                     /gene="yceG"
                     /locus_tag="OO585_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6628150.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(6971..7780)
                     /gene="pabC"
                     /locus_tag="OO585_000455"
     CDS             complement(6971..7780)
                     /gene="pabC"
                     /locus_tag="OO585_000455"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6628151.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(7900..9141)
                     /gene="fabF"
                     /locus_tag="OO585_000456"
     CDS             complement(7900..9141)
                     /gene="fabF"
                     /locus_tag="OO585_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6628152.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9229..9465)
                     /gene="acpP"
                     /locus_tag="OO585_000457"
     CDS             complement(9229..9465)
                     /gene="acpP"
                     /locus_tag="OO585_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6628153.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9676..10410)
                     /gene="fabG"
                     /locus_tag="OO585_000458"
     CDS             complement(9676..10410)
                     /gene="fabG"
                     /locus_tag="OO585_000458"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6628154.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10423..11352)
                     /gene="fabD"
                     /locus_tag="OO585_000459"
     CDS             complement(10423..11352)
                     /gene="fabD"
                     /locus_tag="OO585_000459"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6628155.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11368..12321)
                     /gene="fabH"
                     /locus_tag="OO585_000460"
     CDS             complement(11368..12321)
                     /gene="fabH"
                     /locus_tag="OO585_000460"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6628156.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12389..13459)
                     /gene="plsX"
                     /locus_tag="OO585_000461"
     CDS             complement(12389..13459)
                     /gene="plsX"
                     /locus_tag="OO585_000461"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6628157.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13540..13713)
                     /gene="rpmF"
                     /locus_tag="OO585_000462"
     CDS             complement(13540..13713)
                     /gene="rpmF"
                     /locus_tag="OO585_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6628158.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(13765..14286)
                     /gene="yceD"
                     /locus_tag="OO585_000463"
     CDS             complement(13765..14286)
                     /gene="yceD"
                     /locus_tag="OO585_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6628159.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14485..15069
                     /gene="yceF"
                     /locus_tag="OO585_000464"
     CDS             14485..15069
                     /gene="yceF"
                     /locus_tag="OO585_000464"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6628160.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15181..16140)
                     /gene="rluC"
                     /locus_tag="OO585_000465"
     CDS             complement(15181..16140)
                     /gene="rluC"
                     /locus_tag="OO585_000465"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6628161.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            16426..17445
                     /locus_tag="OO585_000466"
     CDS             16426..17445
                     /locus_tag="OO585_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6628162.1"
                     /translation="MKYTPVGVGIAKHVIQIHFINEHTDEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
     gene            18156..21341
                     /gene="rne"
                     /locus_tag="OO585_000467"
     CDS             18156..21341
                     /gene="rne"
                     /locus_tag="OO585_000467"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6628163.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(21538..22491)
                     /gene="flgL"
                     /locus_tag="OO585_000468"
     CDS             complement(21538..22491)
                     /gene="flgL"
                     /locus_tag="OO585_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6628164.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(22503..24146)
                     /gene="flgK"
                     /locus_tag="OO585_000469"
     CDS             complement(22503..24146)
                     /gene="flgK"
                     /locus_tag="OO585_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6628165.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(24212..25153)
                     /gene="flgJ"
                     /locus_tag="OO585_000470"
                     /pseudo
     CDS             complement(24212..25153)
                     /gene="flgJ"
                     /locus_tag="OO585_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(25153..26250)
                     /gene="flgI"
                     /locus_tag="OO585_000471"
     CDS             complement(25153..26250)
                     /gene="flgI"
                     /locus_tag="OO585_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6628166.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVSGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(26262..26960)
                     /gene="flgH"
                     /locus_tag="OO585_000472"
     CDS             complement(26262..26960)
                     /gene="flgH"
                     /locus_tag="OO585_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6628167.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANVSNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(27013..27795)
                     /gene="flgG"
                     /locus_tag="OO585_000473"
     CDS             complement(27013..27795)
                     /gene="flgG"
                     /locus_tag="OO585_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6628168.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(27933..28688)
                     /gene="flgF"
                     /locus_tag="OO585_000474"
     CDS             complement(27933..28688)
                     /gene="flgF"
                     /locus_tag="OO585_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6628169.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(28708..29916)
                     /gene="flgE"
                     /locus_tag="OO585_000475"
     CDS             complement(28708..29916)
                     /gene="flgE"
                     /locus_tag="OO585_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6628170.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(29941..30636)
                     /gene="flgD"
                     /locus_tag="OO585_000476"
     CDS             complement(29941..30636)
                     /gene="flgD"
                     /locus_tag="OO585_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6628171.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(30648..31052)
                     /gene="flgC"
                     /locus_tag="OO585_000477"
     CDS             complement(30648..31052)
                     /gene="flgC"
                     /locus_tag="OO585_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6628172.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(31056..31385)
                     /gene="flgB"
                     /locus_tag="OO585_000478"
     CDS             complement(31056..31385)
                     /gene="flgB"
                     /locus_tag="OO585_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6628173.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            31541..32200
                     /gene="flgA"
                     /locus_tag="OO585_000479"
     CDS             31541..32200
                     /gene="flgA"
                     /locus_tag="OO585_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6628174.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            32276..32617
                     /gene="flgM"
                     /locus_tag="OO585_000480"
     CDS             32276..32617
                     /gene="flgM"
                     /locus_tag="OO585_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6628175.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSTSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADAL
                     INEAQQDLQSN"
     gene            32622..33038
                     /gene="flgN"
                     /locus_tag="OO585_000481"
     CDS             32622..33038
                     /gene="flgN"
                     /locus_tag="OO585_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6628176.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(33078..34613)
                     /gene="murJ"
                     /locus_tag="OO585_000482"
     CDS             complement(33078..34613)
                     /gene="murJ"
                     /locus_tag="OO585_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6628177.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(34780..35646)
                     /locus_tag="OO585_000483"
     CDS             complement(34780..35646)
                     /locus_tag="OO585_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6628178.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(35648..36295)
                     /locus_tag="OO585_000484"
     CDS             complement(35648..36295)
                     /locus_tag="OO585_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6628179.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(36306..36890)
                     /gene="rimJ"
                     /locus_tag="OO585_000485"
     CDS             complement(36306..36890)
                     /gene="rimJ"
                     /locus_tag="OO585_000485"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6628180.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            37126..38334
                     /gene="mdtH"
                     /locus_tag="OO585_000486"
     CDS             37126..38334
                     /gene="mdtH"
                     /locus_tag="OO585_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6628181.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            38398..39045
                     /gene="grxB"
                     /locus_tag="OO585_000487"
     CDS             38398..39045
                     /gene="grxB"
                     /locus_tag="OO585_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6628182.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            39179..39739
                     /locus_tag="OO585_000488"
     CDS             39179..39739
                     /locus_tag="OO585_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6628183.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            39845..40891
                     /gene="pyrC"
                     /locus_tag="OO585_000489"
     CDS             39845..40891
                     /gene="pyrC"
                     /locus_tag="OO585_000489"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6628184.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            40965..41210
                     /gene="dinI"
                     /locus_tag="OO585_000490"
     CDS             40965..41210
                     /gene="dinI"
                     /locus_tag="OO585_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6628185.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            41500..41754
                     /gene="bssS"
                     /locus_tag="OO585_000491"
     CDS             41500..41754
                     /gene="bssS"
                     /locus_tag="OO585_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6628186.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            41869..42987
                     /gene="solA"
                     /locus_tag="OO585_000492"
     CDS             41869..42987
                     /gene="solA"
                     /locus_tag="OO585_000492"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6628187.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCTGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            43035..43148
                     /locus_tag="OO585_000493"
     CDS             43035..43148
                     /locus_tag="OO585_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6628188.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            43409..43975
                     /locus_tag="OO585_000494"
     CDS             43409..43975
                     /locus_tag="OO585_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6628189.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            43979..44554
                     /locus_tag="OO585_000495"
     CDS             43979..44554
                     /locus_tag="OO585_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6628190.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGNDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(44596..>45172)
                     /locus_tag="OO585_000496"
     CDS             complement(44596..>45172)
                     /locus_tag="OO585_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6628191.1"
                     /translation="EYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGG
                     IRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISD
                     EIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQR
                     RRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT        11035 a        12076 c        11236 g        10825 t
ORIGIN      
        1 tagaagtagt gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa
       61 ttgatatggt tcgaaaacac gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat
      121 acggtagctg gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg
      181 gtaaaatccg cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct
      241 acaacagtga aaagaccttc ttctccggca ttgtcgatgc tgatttaggc gatcttacga
      301 cgctttcagc cggttacgaa tatcagcgca ttgatgttaa tagccctacc tggggcggtt
      361 taccgcgctg gaatactgat ggcagcagca acagttacga tcgtgcacgc agtaccgcac
      421 ctgactgggc gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt
      481 ttgctgatac ctggcaagcg acactgaatg ccacccactc tgaagtcgaa tttgacagca
      541 aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca
      601 gtaattatgg acctggcttt gattatgtcg gcggcaccgg ttggaacagt ggcaaacgta
      661 aagttgatgc gctggatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca
      721 atctaatgtt tggtggcagt tacagcaaac aaaacaatcg ttacttcagt tcatgggcca
      781 acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaaaccg
      841 actggtcacc acagagcctg gcacaggacg ataccacaca tatgaaatcg ttatatgctg
      901 ccactcgagt cacccttgcc gatccgctgc atctgatcct cggcgcacgt tataccaact
      961 ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc
     1021 tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct attttccagc
     1081 cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg
     1141 agctgggtct gaaatcgggc tggatgaata gccgtctgac caccacgtta gccatcttcc
     1201 gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg
     1261 aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg
     1321 gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatatt gcagaggata
     1381 acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg ttcaccagct
     1441 atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgtgaactgg caaaatcgcg
     1501 tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc
     1561 tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca
     1621 ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc tacggcgcac
     1681 cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag
     1741 ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg atgtactcat
     1801 ccatctcggt tttcaggtta tcggatttag taccgaaaat cgcctgaaca ccagaacccg
     1861 caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat
     1921 cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac
     1981 caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct tttgcatctt
     2041 cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga
     2101 tggtgtagta aacaatcgca taaccgatac cgacgatcgg gaacagccac agtttgctgc
     2161 tgttaccaga cagaacgata aagtcgatca gaccgtgcga gaacgacgta ccgtcatgca
     2221 tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca
     2281 ggatcggcgc aacgaacatg aaggagaact cgatcggctc ggtgataccg gtcaggaacg
     2341 aggtcagcgc cgcggagatc ataataccgc ccactttcgc gcggttttct ggtttagcag
     2401 agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag
     2461 acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct
     2521 gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt
     2581 gcagaccaaa cggtaccagg caacgttcga tgaaaccgta aatgccaaac gcaactaccg
     2641 ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa
     2701 tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc
     2761 gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca
     2821 tgtacgctgc gatcgcaccg gagataatcc ctccgagtac gccagtatcc gctaggtgtt
     2881 tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt
     2941 taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa
     3001 agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg
     3061 cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc
     3121 ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat ttaccgacct
     3181 tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag tatgggtgct
     3241 ttttttacgt tctcacgcat ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag
     3301 cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca
     3361 cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat
     3421 ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt
     3481 aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt
     3541 gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt
     3601 tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc
     3661 gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga
     3721 cataacgcgc agcatcgcgc agttgctccg cattacggaa ggtcacaatg ccggaaaagg
     3781 agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt
     3841 gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac
     3901 gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat
     3961 ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg
     4021 tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt
     4081 agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc
     4141 ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt
     4201 tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat
     4261 aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga
     4321 tgaggaaccg gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg
     4381 gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg aatgtggcaa
     4441 acatccccca gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc
     4501 aggcccggca catcaacatt ggtcacctgt gcagcgccat gatggcgttt tagcgcatcc
     4561 atcagcaacg ttgccagcca gtgtaaacgc gccggagctt gttcatgatt aagggccgcc
     4621 agcagcgaat accagtcgcc cgatggtacg ctatatgcca acgcctgaca caatgtttca
     4681 cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgctaggcga accggcactt
     4741 aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaggtc
     4801 acggcgtact gttccggcgg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt
     4861 gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct
     4921 tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc atcggttacc
     4981 cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt gacctcacgt
     5041 accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta gtaatcggga
     5101 tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc
     5161 ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc
     5221 ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt
     5281 ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc ttcacccagt
     5341 gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg
     5401 tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga
     5461 aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc
     5521 caacttccgg ggtaacatcg agatagagcg ttaagtcagg gcgaaaatcc ccgagaacag
     5581 catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct
     5641 gagtggagag atcgtggcga tcgccaatca cccaggtgcc gttagccaga gctggtttga
     5701 tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg
     5761 taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa
     5821 gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcaagcg
     5881 tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga
     5941 tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta
     6001 tggctggcaa gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga
     6061 tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc
     6121 gcacctggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct
     6181 gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc
     6241 tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca
     6301 ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg gtttttatct
     6361 ttataaggca ggccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc
     6421 ttgtgcgctc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat ccaggtgtct
     6481 ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg
     6541 gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg
     6601 tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt
     6661 tccagcaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct
     6721 ttaaagtgag aaagatccgg ttcgatacgc agcaaccatt gaaaaacccg tgggcgattg
     6781 atgatcttat cggcataaag ctgttcacca agcgccagac gtccggtccc tggcttcagg
     6841 gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag
     6901 acgcccacac cagcggcgat acccagtacc accagcaata acaagataat caataacact
     6961 tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg
     7021 acgaaaagga gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa
     7081 taaccatctc atctgcctgc aacgcctctt ccagagaggc ttgcacttcg acaagctgat
     7141 aagaggattg tgccagcaaa cggatacaga attgtcgcat aatgccgtta acacctgcct
     7201 gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta gccgcacagc
     7261 attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa
     7321 gatgagagcg gatcaatact tgctcaagcc gattaagatg tttaatacct gcaagatggg
     7381 gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt
     7441 cgtaatgtgc aggataaacc gtaacggaga gaatccgcgt tgccggtcca ctgttcaatg
     7501 tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca ccattttgct
     7561 gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca cagaaaatca
     7621 tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc cgataacaaa ctgactttac
     7681 cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta
     7741 ctgccagcga ttcctgctta tgaccgttaa ttaagaacat accggctcct tatcagagaa
     7801 ttgttagtgt ggcagcatgc tcactacgga acaagtcgga ataaaagcta agaaaaaagg
     7861 cccgcaagcg gaccttttat atgggtggga aatgacaact tagatctttt taaagatcaa
     7921 agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac
     7981 ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccaggt tatccaggtt
     8041 gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag actctactgc
     8101 ccccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca acacacggct
     8161 tgcagcttca ccgaagatgg ctttcaccgc ctgcgcttca gctttatcgc cagccggcgt
     8221 agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag
     8281 agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata
     8341 agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt
     8401 cgcgtgttcg tactcttcta gtaccagcat accggcacca tcgcccagta cgaagccatc
     8461 acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc
     8521 acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg cgccacctgc
     8581 aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatgaccaa tgttgtgcac
     8641 gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt
     8701 cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg
     8761 tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc
     8821 ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc
     8881 ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg
     8941 ttctttgcgc gagataatgt cctcacagtt aaaatccttt actaagccag caaatttcgt
     9001 tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag
     9061 agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac
     9121 aactacacga cgcttagaca cgtttgtcct ccagggaggg aaaaaatgat tctagtggga
     9181 caaaaagata aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg
     9241 gccgttgatg taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg
     9301 aatctcagta tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc
     9361 cgcgcccagg tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag
     9421 ctgttcgccg ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct
     9481 atcaaaactc gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact
     9541 aaatcccggc aggtcttacc acgattttac gttattttga ggccttaccc cccaataacg
     9601 caaatcattt tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt
     9661 ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt
     9721 gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc
     9781 gccgaggcga cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc
     9841 acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga
     9901 cgcaacttcg cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta
     9961 gttggcctga ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc
    10021 atgacgcttt ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag
    10081 gttggtttcg ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg
    10141 agtgataccg gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc
    10201 cagaacagat tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttagc
    10261 acctaaataa tcactgatcg cctgagcgcc attttcactg gtcgcagtgc caataacttt
    10321 cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt
    10381 taccagtgcg atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc
    10441 tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt
    10501 caggccagtt agcactttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc
    10561 catgtactca acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc
    10621 acgaatggca tcaccattgg tttcgcattt cacatcaacg ttattcacaa caggaactgt
    10681 tggtgcgtta aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat
    10741 cagcgcacag tgagacggta cgctcactgg taacggcagc gcgcgttttg cgcccgcagc
    10801 tttacaggca gcgccagcac gctcaaccgc ctctttatga ccggcaataa ccacctgtcc
    10861 cggagagtta aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt
    10921 cgcaatagac gcatcatcca gaccgatgat tgccgccata gcgcccgtgc cttctggtac
    10981 ggcttcttgc atgaacttgc cgcgcatctc aaccagacgc accgcatcag cgaaatcaat
    11041 cacgccagcg caaaccaacg cggagtattc ccccaggctg tgaccggcca tcattgccgg
    11101 tgctttaccg ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc
    11161 tggctgggtt tgccaggttt tattcagttc ttcagctggc ccctgctggg tcagcgccca
    11221 caggtcgtag cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct
    11281 cgccgccata tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc
    11341 aaattgcgtc atgttttaat ccttatccta gaaacgaacc agcgcggagc cccaggtgaa
    11401 tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac
    11461 agcttcatcc agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccaacgt
    11521 caccacgaca ttgtccatag acataccgag ttttttcgcc gttgcactga taatacgcag
    11581 gttagcctga tgcggaacca gccagtccag ttgagaacgg tcaagattat tcgccgccag
    11641 cgtctcatca acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc
    11701 catcgtcaga tgaattgaat tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa
    11761 ttcaccataa ctaccgtcgg catgcagatg ggtggagatg attcccggct cttcagaggc
    11821 agccagcacc gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg
    11881 atcgcaggtg cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga
    11941 tttcacgtat tgattggcta cgcttaatgc ataggtgaaa cctgcgcagg ctgctgcaac
    12001 gtcaaatgcc gggcaacctt taatgcccaa catgctttga atctgacaag ctgcgctcgg
    12061 gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat
    12121 gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt
    12181 tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt
    12241 gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt
    12301 accaataatc ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc
    12361 aaactgcgag ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca
    12421 gcagctcaaa accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt
    12481 gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg
    12541 caccatgact ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt
    12601 cggggttgag gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca
    12661 gtagccacca cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc
    12721 tgacaacacc ttccatcgtc tttaacgtga catttcctgt aaagccgtca caaaccagca
    12781 catctgtctt gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag
    12841 ggattgtttt aagcaccgct gaggcatccc gaatactgtc gagacccttt acttcttctt
    12901 caccaatatt gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagaa
    12961 ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta
    13021 aatcgaggac caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac
    13081 gctcaatccc ctccaggggc ctgagtaata attttgccag ccccatcagt gccccggtat
    13141 taccggcact gacacaggct tgcgctcgac cttctttcac cagctccagg gccatacgca
    13201 ttgaactccc acgactagcg cggatagctt gcgaaggccg ggcatcactg gcgataactg
    13261 actgcgcagg aataatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg
    13321 gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg
    13381 cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg
    13441 ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg
    13501 gggaaaatcc tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg
    13561 gccgcggtag taaccgtcgg cagtgatatg gtgacgcagg tgtttttcac cagaagtttt
    13621 gtctacagac aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt
    13681 ggaacgggtt ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat
    13741 ccccgatcca acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg
    13801 gtttggcttt tgcacttctt caggcagttc accaaagacc atgtccgctt cggacacttc
    13861 acagtgttca gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac
    13921 cattgcaagc agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg
    13981 cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg
    14041 atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc
    14101 gtttaacact gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact
    14161 gtccacactg actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg
    14221 gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga gtcaggggta attttacctt
    14281 ttgcataggg cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg
    14341 tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt
    14401 gctgataacg aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat
    14461 acgctatatc tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccatgg
    14521 cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc agaggtcgac
    14581 gaaaccccac gcagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa
    14641 gcgcaatctc tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt
    14701 gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta
    14761 cgcaaagcca gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg
    14821 aatgggcatc tacaaacaga agtggagcct tttgacgtcc atttccgtca cctgtgcgag
    14881 gcggagattg ataattacgt gcgtaaggag catcccctgc actgcgcggg tagctttaag
    14941 agtgaaggat ttggcattac gctgtttgag cgtttagagg gacgtgaccc taacacgctg
    15001 gttggtttac cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg
    15061 atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc
    15121 ctgatgcggc gtaaacgcct tatcaggcca accgcccatt atcaggctta tatgcttgta
    15181 ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc
    15241 gatacgcatc acctcaccgg tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa
    15301 cagacgattt aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta
    15361 gcgatcgtca aaggcaatcg gatgacccgc atactgtgta tgcacacgga tctgatgagt
    15421 acgtccggtt accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt
    15481 aaagcgtgtt tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg
    15541 cagaatattt ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc actgactgcg
    15601 caccagcgcc aggtaatctt tttgcattcc tttttcacgt aattgctcat gcagagaacg
    15661 caacgccgag cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg
    15721 aaccagttca aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa
    15781 gcttaaacca ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg
    15841 atcatcttca tataagatga cgtccgccag cgccgccacc ttttgcagat gtggcgaaac
    15901 cgcctcttct tcccgctcag caacgcgaac cggtgggata cgcacctcat cacccgcttc
    15961 gagtttatat tcaggcttaa tacgtttttt gttcacccgc acttcgcctt tacgcaaaat
    16021 acggtaaatc atactttttg gtacgccttt caattgggta cgcaaaaagt tgtcgatacg
    16081 ttgccccgct tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat
    16141 ggtgggcgat tctaattagc caacaggatt cgcgccactc atttttctat gcttatattt
    16201 agttttacac cttattactt cactacgtga tcactttatt gatggttatt aaaccaatca
    16261 ccagcaagaa gtgaaaaatg taatgaacgc atcccacaca attaaacacg gagcagtatg
    16321 atggttaaaa gggtgaaact ggcaggtgtg gagcctcgca gatttccggc atcatagtgc
    16381 ccaatacggg atgcataaag ctccctcaga ctggagactc cacaaatgaa atatacaccg
    16441 gttggcgttg gtatcgcaaa acatgtcatt cagattcact tcatcaatga gcacacagat
    16501 gaagtggttg ataaacagtt gcgtagacag gattttctga cgttcttcgg caaccgtgag
    16561 ccatgcctga ttggtatgga ggcctgtgga ggttctcagc actgggcacg ggaactgaca
    16621 aaacttggtc ataaagtccg gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc
    16681 aataagaatg atgtgatgga tgcccgggct atctggatgg cggttcagca gccgggtaaa
    16741 gaaatcgccg taaaaacaga agaacagcag tcggtactgg ttctgcaccg tacccgcatg
    16801 caactggtga agttccggac cgcacaaatt aatgccctgc acgggacgtt actggagttt
    16861 ggtgaaacca tccacaaagg ccgggcagcg atggagcggg agttccccga agcactggaa
    16921 cggatgaaag agagactgcc accgtatctc attatggttc tggaaaacca gtacaaccga
    16981 ctgaatgagc tggactcact gatagaggat attgaaaaac agcttaccag cgtggcgagg
    17041 cagaatgaaa cctgtaagcg gttgctggat attcctggcg ttggaccact tattgcgacg
    17101 gcagcggtgg ccaccatggg ggaagcatca gcgtttaaat cggggcgaga gttcgccgca
    17161 tatgttggtc tggttccaaa acaaactggc tccggaggga aagtacgtct gctggggata
    17221 agcaaacgtg gtgacactta tctcaggaca ttatttatcc acggtgcaag agcggtggca
    17281 ttagtagcta aagagcctgg cccgtggata accgaactga aaaaacgtcg tccagccagt
    17341 gtggcaatcg tcgccatggc aaacaagctg gcacgaacag tatgggcgat aaccgcccat
    17401 gaccgtaagt atgacaggaa ccacgtcagt atcagaccat attaatcgct gataccatta
    17461 aacaatgaac tcttaacaaa agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat
    17521 gacaaagaca ggtaagaccg tgactcacta aacctgaaca gtattttggg cttgaagtcc
    17581 gccgtgaaaa taaggggtga gtcggcgaat tacatagggg ctcgcagcgt tacggctgca
    17641 ataaagccgg atataaagct gcaacctacc cgtcatgtca aaacaatgga tgccttgcaa
    17701 acgggatgcg ttcatataaa aactgtgagt aagcgggtga taaatggtaa aagtcatctt
    17761 gctataacaa ggcttgcagt ggaataatga agccgtttcc gtgtccatcc ttgttaaaac
    17821 aagaaatttt acggaataac ccattttgcc cgaccgatca tccacgcagc aatggcgtaa
    17881 gacgtattga tctttcaggc agttagcggg ctgcgggttg cagtccttac cggtagatgg
    17941 aaatatttct ggagagtaat acccagtctg tttctttgat aattgcgctg tttttccgca
    18001 tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg acgataaccg tgaggttggc
    18061 gacgcgacta gacacgaggc catcggttca cacccggaaa ggcgttactt tgcccgcagc
    18121 ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa aagaatgtta atcaacgcaa
    18181 ctcagcagga agagttgcgc gttgcccttg tagatgggca gcgtctgtat gacctggata
    18241 tcgaaagtcc agggcacgag cagaaaaagg caaacatcta caaaggtaaa atcacccgca
    18301 ttgaaccgag tctggaagct gcttttgttg attacggcgc tgaacgtcac ggtttcctcc
    18361 cactaaaaga aattgcccgc gaatatttcc ctgctaacta cagtgctcat ggtcgtccca
    18421 acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt tcagatcgat aaagaagagc
    18481 gcggcaacaa aggcgcggca ttaaccacct ttatcagtct ggcgggtagc tatctggttc
    18541 tgatgccgaa caacccgcgc gcgggtggca tttctcgccg tatcgaaggc gacgaccgta
    18601 ccgaattaaa agaagcactg gcaagccttg aactgccgga aggcatgggg cttatcgtgc
    18661 gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg ggatttaagc ttccgtctga
    18721 aacactggga agccatcaaa aaagccgctg aaagccgccc ggccccgttc ctgattcatc
    18781 aggagagcaa cgtaatcgtt cgcgctttcc gcgattactt acgccaggac atcggcgaaa
    18841 tccttatcga taacccgaaa gtgctcgaac tggcacgtca gcatatcgct gcattaggtc
    18901 gcccggattt cagcagcaaa atcaaactgt acaccggcga gatcccgctg ttcagccact
    18961 accagatcga gtcgcagatc gagtccgcct tccagcgtga agtccgtctg ccgtccggtg
    19021 gttcgattgt tatcgacagt accgaagcgt taactgccat cgacatcaac tccgcacgcg
    19081 cgacccgcgg cggcgatatc gaagaaaccg cgtttaacac caacctcgaa gctgccgatg
    19141 agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct gattgttatc gacttcatcg
    19201 acatgacgcc tgtacgccac cagcgtgcgg tagaaaaccg tctgcgtgaa gcggtgcgtc
    19261 aggaccgtgc gcgtattcaa atcagccata tttctcgctt tggcctgctg gaaatgtccc
    19321 gtcagcgcct gagcccatca ctgggtgaat ccagtcatca cgtttgtccg cgttgttctg
    19381 gtactggcac cgtgcgtgac aacgaatcgc tgtcgctctc tattctgcgt ctgatcgaag
    19441 aagaagcgct gaaagagaac acccaggaag ttcacgccat tgttcctgta ccaatcgctt
    19501 cttacctgct gaatgaaaaa cgttctgcgg taaatgccat tgaaactcgt caggacggtg
    19561 tgcgctgcgt gattgtgcca aacgatcaga tggaaacccc gcactaccac gtgctgcgcg
    19621 tgcgtaaagg ggaagaaacc ccaaccttaa gctacatgct gccgaagctg catgaagaag
    19681 cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa gcgtccggaa caacctgcgc
    19741 tggcaacctt tgccatgccg gatgtgccgc cagcgccaac gccagctgaa cctgccgcgc
    19801 ctgttgtagc tccagcaccg aaagctgcac cggcaacacc agcagctcct gcacaacctg
    19861 ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt cagcggtagt gaagaaacca
    19921 aaccgaccga gcaaccagca ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc
    19981 gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg ccgcgacacc cgtagtgaac
    20041 gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa tcgtcgccag gcacagcagc
    20101 agactgccga gacgcgtgag agccgtcagc aagctgaggt aacggaaaaa gcacgtacca
    20161 ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg ccgtaacgat gataaacgtc
    20221 aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca atctgttcag gaaaccgaac
    20281 aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca gcgtcagctc aatcagaaag
    20341 tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc accggtggtt gaagaaactg
    20401 ccgctgccga accaattgtt caggaagctc cagctccacg cacagaactg gtgaaagtcc
    20461 cgctgccagt cgtagcgcaa actgcaccag aacagcaaga agagaacaat gctgataacc
    20521 gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc tcgtcacctg cgcgtgagtg
    20581 gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac ccagtcgcca atgccgttga
    20641 ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt ctggatccgc tatccaattg
    20701 tacgtccgca agatgtacaa gttgaagagc agcgcgaaca ggaagaagta caagtgcagc
    20761 cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc ggttgttagc gcgccagttg
    20821 ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt tgccgaaccg caaccggaag
    20881 tggttgaaac gacgcatcct gaagtgattg ccgccgcggt aactgaacag ccgcaggtga
    20941 ttaccgagtc tgatgttgcc gtagcccagg aagttgcaga acacgcagaa ccggtggttg
    21001 aaccgcagga agagacggca gatattgaag aagttgccga aactgctgag gttgtggttg
    21061 cagaacctga agttgttgct caacctgccg cgccagtcgt cgctgaagtc gcagcagaag
    21121 ttgaaacggt agccgcggtt gaaccagaga tcaccgttga gcataaccac gctaccgcgc
    21181 caatgacgcg cgctccggca ccggaatatg ttccggaggc accgcatcac agtgactggc
    21241 agcgccctac ttttgccttc gaaggtaaag gtgccgcagg tggtcatacg gcaacgcatc
    21301 atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta ataattagct caaagtaatc
    21361 aagccctggt aactgccagg gcttttttat ttcatgtttg aatcaacata ttaaaattca
    21421 tcaaatggca tccttgctaa ccaacaatgc aaaataggca aaaaaaatgc ccgccccggc
    21481 gatccctgag cgggcagaca aaacataccc agtttcatga tatgttttaa agcgagctta
    21541 tttgttgagc tggaagagcg acaatccctg catatcggta aatgctttat acgatgcctg
    21601 caatgccgtt tgctgcatga tgtaagatga aatagttgca ttccagtcca catcaaccag
    21661 atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta cctaaggaat ccagcgactc
    21721 cagttcgttc agctgcgtgc ctaattccgc gcgaacggca aggacattat tcagcgagtt
    21781 tttcagtcca cggttggttt tatccaatgc cgcagcggcg atttctttat ccgcttcgct
    21841 atccgcgacc ggcgttttca gggctgcgat ggcgctatcc agcatggcaa aaagattggt
    21901 ttcagaagcg ctaccgtctg gctccgcaac tgcgttgctg gtaatactgt cgaaaatttt
    21961 gtcacccgtg tgccctatca ccatcgaacg cgaagcatcg acctgttgct taatactttc
    22021 tgctccaccg acgtatttcc ctttctcttc gctaaacggc gcagtctctg ttttataacc
    22081 ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc agattcagca actggtcacg
    22141 aagcccctga atatccgtag ccagcgaggc ccggtcatcg tcactcaagg tgccattgct
    22201 ggcgtagaca attttttcct gagcattctg gatagcagtg gtgacctggc taagtacact
    22261 ctcttccagt gacacttttt gaatggcgaa agtacgcgcc agcgtgtact ggctgttttg
    22321 cgcctgtgcc tgggagagaa ctacggcttg tgatgctgca atgggatcgt cagaagggtt
    22381 aacgactcgc ttacctgtcg acatctgctc gccgtacttc atccattccg cctgagaatt
    22441 ggtgatgcca cgcatgtttt gctggtacat catctgggta ctgaaacgca tcttatctcc
    22501 ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg ccgtctgcag aacctgcgca
    22561 ttcgccaggt aatactgctg aaaacgttgc agatttccgt actcttcgtc gagattgacg
    22621 ccagagatag actgctgctg attggaaagc tgcgtcacca cattaccttg cgtggcgcta
    22681 ctggttttca acgtcgcggt tttattaccg atatcactca ctaacgaggc ataagcgtcg
    22741 ttaaaggatt tcgcaccgcc caccgtttta ctgttgcttt gcagatccag cagggcctga
    22801 ccgttgcggt tatcgctatc acccgcatct ttttcgctcg ccatcgctat tttcgcttcg
    22861 tcggtgatta atacatccat gttgacgatg gcgtcactta ctggtttcag cgtgaagctg
    22921 tcgttaacgg caggcgttcc tgtaaacgtc aactccagac catcaaatgc cactttaccg
    22981 ttggcatccg gcgtcaccgt aaaagtggta ttgctggcaa ggcgggtgac ctgccactga
    23041 ttattatcga acgagatttt gtaatctgtc gccagtaccg cggaggcatc agttaccgtg
    23101 gcaccgatcg caacgtcacc tttgtttttc gtgttttgca aaaccgcggg cttaccgata
    23161 gcaaagaaat cttcaccagc atcgccgttg gcatcaaatc cggctttgtg ttgggtgttg
    23221 aaagcctcgg caaatgccag cgccagttgt ccaagcgtat tacgcgtctg gtccagatcc
    23281 tgagaacgga atgtcagaat gccgcccagc gacccggtat tcagtaattt ctccgggatc
    23341 tcaatattgc ctgccgtccc atcaacgtaa gcgacagtcg tacgagaagg gtcggcgctg
    23401 gaaggaactg ccgccagttg ccgcgccgta cttccctgaa ccagtgagta accattggcc
    23461 atcgtgatgt tataagtgcc gccatcctga acgctgactt ctacaccaac aatctggttt
    23521 aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgacgc ccctgccccc
    23581 acgcctgtca ggcgggagat ttggtcgttc aggctggcga tttgtttagc gtaattgttg
    23641 atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc acgaagatac
    23701 tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag cgcctggcgc
    23761 gctgccgggt cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa atcctgcatc
    23821 tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga catctgctca
    23881 tagcgggcag tcaggccgct actttgcgtc tgcgccgcac gtaactggtt ggtgataaac
    23941 gcatcatact cacgctgcac accagaaacg tagacaccat tgccaaccca gccgccagcg
    24001 cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc ggcaacgtta
    24061 tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt cagtccgctc
    24121 atggcgttat taatcaagct ggacatggag gttccttata agccttcaat tacagagtat
    24181 tatcggcagc gacccgccgg acttgagtta ttcaaaacag attatcaatg ttcatactgt
    24241 aggttttgct caccttgtcg cttatcgatt tcatctgctg aatcatgttg gtgagtttgc
    24301 gggcatagtg aggatcggtg gcatagcccg cgtcctgtag ggcctgcgcc ccctgctccg
    24361 cactcgtggc ggtggtcacg gcggcgtagc gcgggttacg cgttaacagc ccaacgtaat
    24421 ccgactatgc ttccagatac gagctgtaga cccgaaactt cgcttttact ttcttcgctt
    24481 cgccgttttc atattcagtc gtggtgattt cagtaactgg ccctttccag ttgccagagg
    24541 ctttgacacc aaacaggtta tagctcggct cgccgttttc gcggcggatt tgccgttgcc
    24601 cccaaccaga ttccagcgcc gcctgagcga ggatcaaatg atgtggcaca ccgctttgct
    24661 ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa tgctttactg tcacccggca
    24721 gcgaatcatc gtagttacgt ggcacggcct tttgcaccag ctgcgaaagc gcctgatttt
    24781 gataacgcac cacagtttcg agcgggaatt tcatcggtgc tgctggcgtg gactcctctg
    24841 gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc agacctttgc
    24901 ccgccgtcat ctgttgggca atctgctggt catacatact ggtatacagg cgagtgtgct
    24961 cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac atcatctgca
    25021 cgaacatccc ttccacctga cgggccaccg gacggatatt tgccgccgga tcttcgcccg
    25081 ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc agtagtttgc
    25141 tatcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac tttgcattga
    25201 ttgcagtatg gacatcagat ccatcggcgt agcgcccagc gcattgagcg cgcgcaccac
    25261 gttattgagc ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc
    25321 gatctgcgtt tgtggagtaa ccacagtctg tccaccacca aacggtgtat ctggctggct
    25381 gacattggcc tgacgattaa ctgttactga gagattcccc tgcgctaccg cgcagctgtc
    25441 gagggtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca ctactttagc
    25501 gtcctgcggg gtgacattaa cctgcatatt ctggatatcg gcaaggaagc ggacctggga
    25561 actgttgcca ctcggtacgc gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc
    25621 atatccacgc acgcggttga tggtgtcagc gatttgctgc gccatgctga aatcttcgtc
    25681 gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt cacgttcaat
    25741 aaccgcacca ttggtgatcc gtccaccgtt cagttggtta acctgaacac tgctaccgcc
    25801 agcggaggct cctgcgccgc taaccagaat attgccctgc gccagcgcat acacctgact
    25861 gtcaacgccc ttaagcggtg tcatcaacaa cgtacctcca cgcaagcttt tggcattacc
    25921 catggaagaa accaccacat cgatggtttg cccctgacgt ccaaacggag gaagtgacgc
    25981 tgtcaccatt accgcagcga cgttttttag ctgcatattg gtgcccgtcg gaacggtaat
    26041 tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct gggttgtctg
    26101 gtcaccggtg ccatccagcc ccaccaccag accatagcca atcagtgagt tttgccttac
    26161 cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg tgaccagtag
    26221 aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg cgacaggtta
    26281 aggaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc attgcctacg
    26341 tattcaatgc gcgcatccac cacctgagta gacggtacgg tattgctgcc gctgatagtg
    26401 cgtggattaa ccacgccaga gaagcgaata aattcggtac cctgattaat ggcaatctgt
    26461 ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt caccgtcaac
    26521 gtgccgctaa aggtattgct gacattggcc ccgccctttc cgttgaacgt gttaccaccg
    26581 gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg cggcacagta
    26641 tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct tttgctggcg
    26701 ctgacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct
    26761 tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat agaaccgttg
    26821 gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgcac cagcggcgtg
    26881 gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat ggcataagta
    26941 tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc
    27001 accggttaag ccttagagtt gcgtcagttt ttgctgcatc tgatcggtgg tggacaccgc
    27061 tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt cttccgccac
    27121 gttgacgtta gacgtttcaa catacccttg atacagcaat cccgcgccgt tcaggcccgg
    27181 cgtgctttcg ttcggtgcac cagaggattg cgtttcggtg tagaggtttt cgccaatgct
    27241 ctccagcccg gtgtcattca taaaggtggt gagattgagc tgcccaacct gaaccggagc
    27301 tgcctggcct tgttgggtta cgctgaccac gccatcacga ccgatggtga tacttaacgc
    27361 attcgccgga atggtgatcg ctggctgcac ctgaaaacca ccagccgtca ccagctgccc
    27421 gttctgatcc acctggaaag agccgtcacg ggtataggct gatgaaccat ctggcaacat
    27481 cacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttgttgg tctgcgacag
    27541 atttccctgg ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc cgatttgtaa
    27601 tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa tggtttgata
    27661 aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga cgtttgccag
    27721 gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt tggcgatcca
    27781 taatgaactg atcataaaat gtcctgtagt ggattagtga caagcagagc agaagcacgc
    27841 gacagtgtga attccccctc actctaaccc tctccccaaa ggggcgaggg aatagtccgc
    27901 gcgcttttat tgcaatgtca tcaatcgttc aattaactca tcgacagcag ttggttggca
    27961 cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg
    28021 ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaaccccc
    28081 gacatcacac gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct
    28141 gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc
    28201 agtttcagac gccctactgg cgcaactgta tttgccggat cgcccggatt gagcgccgag
    28261 attgtgccat cggcagcaat agtgatttcc gctccttccg gcacagcaat tggccccgcc
    28321 tcgcctatca cctgatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg
    28381 ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca gccatcctgc
    28441 tgcaacgcaa cgtccagcgg gcgcgaggtg taatctatct tgccgggcgt catgtctgca
    28501 cccggtgttg acgccgttac cagcgtgcgc gtgggcagtg aaagcccttc caccggcact
    28561 gcgcgcaagg cgtttaactg cgcgcgaaaa ccgggcgtag aggcattggc cagattactg
    28621 gcggttaccg cctgttgatt cagcgtctgg ctggctgctc ccatcgcagt ataaattgcg
    28681 tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt tgaggatctg
    28741 gtcctgggtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa cgatcatatt
    28801 gaccagttct ttactgagat cgacgttgga cgcttccagc gcaccgttgg tcagggtgcc
    28861 aaagtttccc gtcccggctg tccccaacag cgccacgcca gaagattgcg tcgcagacca
    28921 gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca gtacaatctg
    28981 ccccagcagt tgagtttgtt cgttggaata gttgccgaca accgtaccgt catcattgat
    29041 ttgataactc accagatcgc ccggtttgta gccgttctgg ggggttgcca caatattgtt
    29101 agcgccggta ttttgctgca tggagttgag gaagctcaga ctaaacgtgg cgggttctgc
    29161 accgttaatt gcgccggttg cgatattatt cgccatcgca ccatccacta atgtgccatt
    29221 agcattaaat tccagtgttg tcgctgtctt cgcaatgctg tttggatcac tgctatcctg
    29281 ggtgtagacc tgccagttat tatccccggt cttcacaaag tagacgctca tgtcatgagc
    29341 attaccctga ctgtcgaaaa cagtcaccga accttttttg ttatagctat ccgcattgct
    29401 ggcgctgaat ggcgtaacag taggaagcgg atcactggaa ttcaggttga tctgcatcga
    29461 cgccgtggtg gtagttttcg ctgccatcag ggtattcggg atcgaaatat tggtcggatt
    29521 cgccccttgc tgaatagtcg gcggcgtacc ggttgccggg taacccgtca gctgtaaacc
    29581 ttgcatattc accaggttac ggttttcatc cagcttaaat tgtccgttac ggctgtagaa
    29641 caccgaaccg ttgctgtcta ccagacggaa aaaaccgttc tggctgatag caacgtccag
    29701 acctcgcccg gtgttggtgg tcgtgccatc tgtaaagtcc tgagtgatac cggcaacttt
    29761 tacccccagt cccactttcg aaccggcaaa catatcggca aaagaggccg tgcctgattt
    29821 aaagccgtag gtggcggagt tggcaatatt gttgccaata acatcgaggt tagtggcggc
    29881 agcgtttaat ccgctaaccg cttgagaaaa ggccatgact gactcctgaa atgtgaaggc
    29941 ttaaattatc tgccgtactt cgtcgagggt ggtggtgccg taagtgccga gatccagcgt
    30001 attaccgctg ttgccgcgga tcacaccctg caccagagca aactgcagcg gctgggcaac
    30061 cagttgtgta ccaccgttac tggcggtaat cgctacattg taagaaccgt tcggcgcagt
    30121 gctgccatca gtcaacgtac cgtcccaggt gaaactgtga actccggcgg tcagttcacc
    30181 aatatcaatg gtgcgcacaa ccgcgccatt tttatcggtg atggtggccg taactttgtc
    30241 tgccgcctgt tgcagctcaa caccaaacgg cgtggtcgtg gtcacagccc cttcttcact
    30301 gccggttccc gcaagaacag ttgtgccggg gatcatcacg ccgtgaccga tcaggttact
    30361 ggcctgtaac gactggctgt tatcaatctg tccggaaata gatccgagca tggtattgag
    30421 tttttcaatc ccgctgaccg tgctgatttg tgccaattgc gacgtcagct cgttgttttc
    30481 cattggattg gtcgggtcct ggtttttcag ctgcgccacc agcaaagtca gaaaactgct
    30541 ttgtaaatct gcggcgttgc tgcccgtgag cgaactactg ctggtggtac tgacgccggt
    30601 atttgtcgga tcggtggtgg ttaccgcaat ggacatagct ttctccttta ttgaccgagc
    30661 gtaagggttt tcagcatcat gcttttcacc gtgttgagca cttcaacatt ggcctgatag
    30721 ctgcgtgacg ccgacatggt gttaaccatc tctccgacaa catcaacgtt cggcattttt
    30781 acgtagccct ttgcatccgc cagcggatta cccggttcat aaaccagttt gtccggggcc
    30841 tgactttcta taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta
    30901 acctggaata ccacctgttt tgcccgatat ggctgtccat cgggaccggt cacgctatca
    30961 gcattcgcca gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac
    31021 ccggcgatat caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc
    31081 atcatgcctt tgatttgccc gctcaacgca ctaaggctca tctggtattg cagttctgcg
    31141 gtaggaggcg tcagcgcctg cgccggaatg tgttgcgttg aggtcatcgt cagagcaacc
    31201 acactggttg catcccgtcc acgttgcatg acttttttaa gttcactggc aaaatcgata
    31261 tcgcgcgcct gataaccaag ggtatcggca ttggcgatgt ttgctgccag cacttcctga
    31321 cgctgggcgc gcagattgag cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg
    31381 agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc
    31441 gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatga ccattgggga
    31501 cgggtacaat tcacgttgta gaaatagctg gggggcgaaa atgctggcaa taaaacgtag
    31561 cgtggcgatc atcgcgatac tgttcagtcc gttaagtgcg gcgagcaatc tcacatcgca
    31621 attgcacacc ttttttagcg cccagctcgt gggggtaagt gatgaggttc gtgtttctat
    31681 tcgtacagca cccaatctac taccgccgtg cgagcagcca ttgctttcga tgagcaataa
    31741 ttcccgcctg tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt
    31801 acaggttaat gtgcaggcca caggaaatta tgtggttgcc gcgatgccca ttgtgcgggg
    31861 aggaaagctg gaagctggca atgtcaaact aaaacgcgga cgactggata ccctgccacc
    31921 gcgtacggtg ctggatatca atcaacttgt tgatgccatt agcctacgcg atctatcacc
    31981 cgatcaacca atccaattaa cccattttcg ccaggcatgg cgggtaaaag cgggacaacg
    32041 cgtcaacgtg atcgccagcg gtgacgggtt tagcgccaac gcagaaggtc aggcgctgaa
    32101 caatgcagcc gtcgcacaga atgcgcggat gcgcatggta tcgggactgg tggtcagcgg
    32161 cgttgttgat gcagatggga atattcttat aaacctgtaa gctgttaaag attacccgtc
    32221 ccttgccgat aaataagcaa cacatgataa aagcgccctc aatgaggaat aaaccatgag
    32281 tattgatcgc acttcgcctc tgaatcccgt aagcaccgtt caaccgcgcg aaaccactga
    32341 cgcgccggta acgaacaccc gggcggcaaa aacaaccgac tccaccagca ccagcaccag
    32401 caccagcacc agcaccagca ccagcaccag caccagcacc agtgtgacgt taagcgacgc
    32461 gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat cttgaacgtg tcgaagcgtt
    32521 aaaactggcg attcgtaacg gtgaactaaa aatggacatc ggcaaaattg ccgatgcgct
    32581 gatcaacgaa gctcagcaag acttgcagag taactgatcg tatgacacgt cttgcagaga
    32641 tccttgacca gatgtccgct gtgcttaacg atctcaaaac ggtaatggat caagagcagc
    32701 aacatctctc tatggggcag atcaacggca gccagttgca atggattaca gaacaaaaaa
    32761 gctcactgct ggcgacgctg gattacctcg aacagttacg caagaaagaa cccaatacag
    32821 caaatagcgt tgatattagt caacgctggc aggaaattac tgtgaaaacg cagcaactac
    32881 gccaaatgaa tcaacataac ggttggttac tggaaggaca gattgagcgc aatcaacagg
    32941 cgctggaaat gttgaaaccg catcaggaac cgacgctata tggggcgaac ggtcagacct
    33001 caacaaccca tcgcggcggt aaaaagattt cgatctgaag attatctccg gcctgcactg
    33061 caggccggaa tgcattgtta caccgtccgg cgggcaaatt ctttaacttt gaagcccagt
    33121 accgccagtg cagcgaagta cgcggcaatc cccgccagca cgaccgccat taaacgcagt
    33181 aagcgccagg gcatggtacc caatgaccac tccggcatga tatgtaacat acctaaaagc
    33241 acgccagaca tcaccagtac cgccaccacc agacgcaaca gaaacgccat ccagccgggt
    33301 tgcggagtaa agattttctg cttacgcaac tgccagtaaa gcagcgaagc attcagacac
    33361 gccgccagac caatagaaag tgacagcccg gcatgtttca acggaccaat aaacgccagg
    33421 ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt tcactggcgt tttaatgtcc
    33481 tggcgggaat aaaagccagg agccaacact ttcactacaa tcaggccaat caaacccacc
    33541 gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa acgcggtaaa tttaccgtac
    33601 tggaacagcg aaacggttaa cggacctgaa agaatgccca acgcaaccgc actcggcagc
    33661 gccaacagga aacaaagacg caacccccag tccatcaaac ggttgtattc atcatgattg
    33721 ccactggcaa aacttttcga cagcgacggc agcaaaatgg tgccaagcgc cacgcccagc
    33781 acaccggacg gaaactccat taagcggtcg gcgtaataca tccaagacac cgaaccggaa
    33841 gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga tctggctcac agagacgcca
    33901 aggatcgccg gtcccatctg tttcaccacg cgcattgctc cggcatcgtg gaagttaatg
    33961 cgcggcagga ccagcatgcc gatcttcttc aggtgcggta gctgatacac cagctgcagg
    34021 atgccgccga ccgtaacagc ccacgccagc gccagcaccg gtgggttaaa gtacggtgcg
    34081 gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg ttggcgcaaa cgccggaatc
    34141 gagaagcggt tccacgtatt cagaatcgct cccaccagcg acgccaggga gatcagcaag
    34201 atatagggaa aggtaatctt tagtagctgg ctggtcaggg caaatttgtc agctgtatca
    34261 gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg cgagcatgcc agcgaccgtc
    34321 acaaccgcca gcgcaagtgt cagcaggcca gaaacataag agacaaagac ccgcgtggcg
    34381 tcttcaccct gcttactttt atattccgcc agaatcggta caaatgcctg ggaaaatgcc
    34441 ccttcggcaa agatacggcg taacaagtta ggaagtttaa aagcgacgaa aaaggcgtcg
    34501 gttgccatcc ctgcgccaaa gattctggcg acaattgcgt ctcgtgcgaa gccaagcaca
    34561 cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt ttaataaatt catcggtgtt
    34621 ctaatccaga ccaacgggca aaacgcctgc aagacaggcg ctgaaagagg cgaatagtct
    34681 acctggatta tggtgaattg ctaccgccag atgttacagg gtttattcac tcatcgcatc
    34741 gcgccagatc ttgtcaacga tacgttgcgc cagcacggcc tattcgccgg cggtttgcgg
    34801 aactgtctga ttttgcacac attcaatgaa gtgccgcgca cagccgacaa atccacgttg
    34861 ttcaagagtg ctctgccaac caggaatcgg tttatgcact acgccctgcc cgcgctcctc
    34921 acgccattcg cgcatatccg taatgtcgat gagcgcaccg tcagtcacgg cctgcacggt
    34981 ttcacgctga cttccggcac ggcgatgcat acaggtggtg atctgcaaag gaccggccga
    35041 aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta gtcagtagcg taccgccatc
    35101 cagagaggct ttgccgcccg acaaccacag cgcggtatcc accacatgca gataatcatc
    35161 cagcaacgtg aaataaagat cgtgtggccc gacactattg ctacgatgtt tatccattct
    35221 tagcgaagct gcggtagcga gttgcgtttt taactcaccg tagagtggtg cgaaacgacg
    35281 gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc agttccacca gccgttcagc
    35341 atcgcgcaga ttttctgcca gcggtttatc gacacagaca tgtacacccg cattgagtaa
    35401 cgtactgacc acgtcaaagt ggctggcggt gctggaatgc acaaaaaccg catcgcaact
    35461 ggcggcaagg ctggataacg aatcggcata aggaatgcgc cagctttcac aaattggcag
    35521 ggctttcgcg cgcgtaggcg accaggctcc ttgtaacatc cagtcagacg ctgccgccag
    35581 caccggtaac cacgcttttt gcgcaatgcc gcctaatccc acaacgccga tacgtaattt
    35641 tttcacttta atctcccaga tgggccagca acgaatcaag acgctgtttc agttctgcca
    35701 cttcgatttc cagggcttcg acgcgggcct gtaaatcacc atcaaccgcg ttcgacatat
    35761 ccgttaccgc cggctgatcc tctacctcac cactgaaaag atgcatgtag cggctttcgc
    35821 gtttacccgg ttcgcgggcc agacgcacca caaaaggacc atcttcgcga tttgccagtt
    35881 gttccagcgt cgactccaat tccgccatat cgctgaattc atacattcgc gcggcgcggc
    35941 tgcgcagttc acccggcgtc tgggcaccac gcaataacaa cgtggtgatt aacgccactt
    36001 ccgctgcgct cagtttcaga tcgccaaatt ctgaattaca aaaacgttgt tcatatttgg
    36061 tgacccgatt accaaagcca ctcaccgtgc gcagataatg gcgtttgacc agattatcca
    36121 gttgctcctg cacttcagat tcactcagat tcatgaccgg ttcacggttc gttttctgat
    36181 tacaggccgt gactacacca ttgactgaga gcgggtattg ctccggcgtg gtcacctgct
    36241 tttccagcaa gcaaccaatc acgcgagctt caagggcagt aagttgatat ttcatgagat
    36301 tctccttagc ggccgggcgt ccagtctggg gtagttaatg ccgtcagtac gtgatcgcgc
    36361 cattgtccat caatcaacag atagtctttc gcatagcctt ctttttcaaa acccagtcgc
    36421 gccagtaaat caccgctgcg tttattgtgc ggcatataat tagccataat gcgatgaata
    36481 tgttgggtgc gctgcatata acgaatggct gcggtcaggg cttcaaacat gagtcctttg
    36541 ccctgccatt tttgcccaat cgaataaccg agatagcagg catgaaaaga gccacgaaca
    36601 acattggaaa aattggcaac gccaataatc tctttttcgt ccgggtcgaa taagccaaag
    36661 tagaaagctg aaccttgttt atgaaattcg ttaatcatcc ccagcctggc ctgccagcct
    36721 gatggataac agtggctttc gtcgcgcact ggctcccagg gcttgaggaa atggcgattc
    36781 tctgcgtaat aatccgcaag acgccaggca tcacgatcat gcaccagacg cacgaccagt
    36841 cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat agccaaacat tctataccta
    36901 ctccttcccg taacgtcttt aatacgctgt aagtattact atacctgcgc aaccagaccc
    36961 tgtgaaaagt ccaaaatgcc attttctgca ttattcacat atttgataaa aactctctat
    37021 caaagcgacg ttttgataaa aaaatattgt ccccgaagtg atgggaaaag tcttagcggc
    37081 aatgcagaat agaaagtgct catcttttat ttcccgggag gggaaatgtc ccgcgtatcg
    37141 caggcgagga acctgggtaa atatttcctg ctcatcgata atatgctggt cgtgctgggg
    37201 ttctttgttg tcttcccgct gatctctatc cgcttcgttg atcaaatggg ctgggccgcc
    37261 gtcatggtcg gtattgctct cggtctacgc cagtttattc agcaaggtct gggtattttc
    37321 ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga ttgttaccgg tatgctgatg
    37381 cgcgccgccg gattcgccac aatgggtatc gcccacgaac cgtggctatt gtggttttca
    37441 tgcctgctct cgggactcgg cggcacgttg tttgatccgc cgcgttcggc gctggtggtg
    37501 aaactaatcc gtccacaaca acgtggtcgt tttttctcgc tgttgatgat gcaggacagt
    37561 gccggtgcgg tcattggcgc attgttgggg agctggctgt tgcaatacga ctttcgcctg
    37621 gtctgcgcca caggggccgt tttgtttgtg ctgtgtgcgg cgttcaatgc gtggttgtta
    37681 ccggcatgga aactctccac cgtacgcacg cccgttcgcg aaggcatgac ccgcgtgatg
    37741 cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg gttactacat gctggctgta
    37801 caagtgatgc tgatgctgcc aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt
    37861 aaatggatgt atgccattga agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc
    37921 tggagtgaaa agcattttcg tctggaacac cggttgatgg ctgggctgtt gataatgtca
    37981 ttaagcatga tgccggtggg catggtcagc ggcctgcaac aacttttcac cctgatttgt
    38041 ctgttttata tcgggtcgat cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg
    38101 gcggacgcaa gagctcgcgg cagctatatg gggtttagcc gtctgggtct ggcgattggc
    38161 ggcgctattg gttatatcgg tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag
    38221 ccagagcttc cgtggatgat gctgggaatt attggcatct tcactttcct tgcgctgggt
    38281 tggcagttta gtcagaaacg cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat
    38341 actcccccct gactcctcca tactgaaagt gaactgacag ccaaatggag gagtcacgtg
    38401 aagctataca tttacgatca ctgcccttac tgcctcaaag cccgcatgat tttcggcctg
    38461 aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg acgcagaaac acccacccgg
    38521 atggtcggtc aaaaacaggt tcccattctg caaaaagatg acagccgcta tatgccagaa
    38581 agcatggata tcgttcacta tgtcgataaa ctcgacggca aaccgttact gaccggcaaa
    38641 cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg gctacgccaa caaactgctg
    38701 ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta ctcccgccgc gcgcaaatat
    38761 ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg acctgctggc ccactctgac
    38821 ggtctgatta agaatatcag cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg
    38881 aacgccgtga atggagaact ttcggaagat gatattcagc tatttccgct actgcgtaat
    38941 ctgacgctgg tagccggaat caactggcca agccgcgttg ctgattaccg cgataatatg
    39001 gcgaaacaga cacaaatcaa tttgttatca tcaatggcga tttaatcctg acccgccgcg
    39061 gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt ctgctggcga gtttatcgct
    39121 ggtgacacat gctgcgttgt gtcaggatat tgacgcagat ttcatgtcga ggactaccat
    39181 gaacaagttt ttatttgccg ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca
    39241 actcacccaa tacaccatca ccgaacaaga aattaaccag tcgcttgcga aacataataa
    39301 tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc catattgttc tgacaaactt
    39361 gaccagccaa attggtcgcg aagagccgaa taaggttacc ctaaccggag acgccaatct
    39421 ggacatgaac tccctgttcg gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct
    39481 gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc
    39541 gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg ttgcttccct atttgaacca
    39601 ggcattacgc aattacttta accagcaacc tgcttacgtc ctgcgcgaag atggcagtca
    39661 gggcgaagca atggcgaaaa aactggcgaa aggcattgaa gtgaagccag gcgaaattgt
    39721 cattccgttt acggattaat gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg
    39781 aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatc ccttcagccg gagcatagag
    39841 attaatgact gcaccatccc aggtattaaa gatccgccgc ccagacgact ggcaccttca
    39901 cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat accagcgaaa tttatggacg
    39961 ggctattgta atgcccaatc tggctccgcc cgtgaccact gttgaggctg ccgtggcgta
    40021 tcgccagcgt attcttgatg ccgtacctgc cgggcacaat ttcaccccat tgatgacctg
    40081 ttatttaaca gattcgctgg atcctaatga gctggagcgc ggatttaacg aaggcgtgtt
    40141 caccgctgca aaactttatc cggcaaacgc aaccactaac tccagccacg gcgtgacgtc
    40201 aattgacgca atcatgccgg tacttgagcg catggaaaaa atcggtatgc cgctactggt
    40261 gcatggtgaa gtgacacatg cagatatcga catttttgat cgtgaagcgc actttataga
    40321 aagcgtgatg gaacctctgc gccagcgcct gactgcgctg aaagtcgttt ttgagcacat
    40381 caccaccaaa gatgctgccg actatgtccg tgacggaaat gaacggctgg ctgccaccat
    40441 cactccgcag catctgatgt ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca
    40501 cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa caggcattgc gtgaactggt
    40561 cgccagcggt tttaatcgag tattcctcgg tacggattct gcgccacatg cacgtcatcg
    40621 caaagagagc agttgcggct gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta
    40681 cgctaccgtc tttgaagaga tgaatgctct gcagcacttt gaagcattct gttctgtaaa
    40741 cggcccgcag ttctatggct tgccggtcaa cgacacattc atcgaactgg tacgtgaaga
    40801 gcaacaggtt gctgaaagca tcgcactgac tgatgacacc ctggtgccat tcctcgccgg
    40861 ggaaacggta cgctggtccg ttaaacaata aaaatcgtag cttcctgttg tcattaggtt
    40921 attttacctg tataaataac cagtatattc aacagggggc tattatgcga attgaagtca
    40981 ccatagcgaa aacttctcca ttgccagctg gggctattga cgccctggct ggcgaacttt
    41041 cccgccgtat tcagtatgcg tttcctgata atgaaggcca cgtatcggta cgttatgccg
    41101 cagcgaataa tttatcggtt attggcgtaa caaaagaaga taaacagcgc attagcgaaa
    41161 ttctccagga aacgtgggaa agcgccgatg actggtttgt cagcgaataa tatgcagtga
    41221 tttttgtttt tgccgggtcg ccccggcttt tttattttac ttacaaattt tgagtacaaa
    41281 atttctacac ttcctcaaaa taattgcaca atatgctaaa aaaatatgaa tgacatgctg
    41341 ttttattgtt catataaatc ttaaaagcag aaatttatct gttcccccct ttaaaaattc
    41401 ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct
    41461 cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc
    41521 attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg
    41581 atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt
    41641 cagacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga
    41701 atcgccaaaa ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac
    41761 atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct
    41821 gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat
    41881 ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc
    41941 ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccac
    42001 ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc
    42061 ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca acgaagatga tcccattttt
    42121 gtacgctctg gtgtcattaa cctcggcccg gctgactccg catttctcgc caacgtcgcc
    42181 cacagcgccg aacagtggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc
    42241 cgctggccag aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt
    42301 tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt
    42361 gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg cgtaactatt
    42421 gaaaccgttg acggtgagta tcaggcgaaa aaagcgattg tctgcacggg aacatgggta
    42481 aaagacctgc tcccggagct gcctgtccag cctgtacgta aagtatttgc ctggtatcag
    42541 gccgatggcc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat
    42601 ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac
    42661 ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat
    42721 ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac
    42781 ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc
    42841 ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca
    42901 gttttagggg aaatagctgc cgattttgcg caagacaaaa aaagcgattt tgatttgacg
    42961 ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt
    43021 tacaggagta tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc
    43081 tgatgcttta tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt
    43141 atttttagtt ttccgcactg acataaactg acaaatattt aatctccctt tcatttagcg
    43201 tgaaaaattg aatgaaatta ttcctgcttc tacagcactc accagacatc tctgatgctg
    43261 aacccactaa acttatgaag attctttcat tattaattca aaattattga aataaaaata
    43321 aatatcattc aatttgttat ttttgtgttg caattcattc tccgacacgc cattttcttt
    43381 ctctcgcaaa ttcgtattgg atgcacttat gtcattcaca aatacccctg aacgctatgg
    43441 tgttatatct gcggccttcc attggctaag cgcaattatt gtctacggca tgtttacttt
    43501 aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag cacccgaact
    43561 gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt
    43621 catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa
    43681 ggctggacat ctcgcattgt acctattgct gttcgccatc ggcatcagcg gctatctgat
    43741 ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct
    43801 tgccgacgct ggcgcacagg cagactttgc cggtgccctg catttttggc tcgcgtggag
    43861 cgtcgtagta ctgtccgtta tgcacggatt tatggccctg aagcatcatt tcatcgataa
    43921 agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag tataaaaaat
    43981 gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg ttctctgccg gttcagcggt
    44041 tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaatt tccgcatcca
    44101 gcaccttggc tatagctggt tatacggcac ctttaaagat ttcgacggta cttttacctt
    44161 tgacgaaaaa aatccggctg ccgataaagt gaatgtgaca attaacacca ccagcgtcga
    44221 tactaatcac gccgaacgcg ataaacatct tcgcagtgca gatttcctca ataccgcaaa
    44281 atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg aactggatat
    44341 taccggcgat ctgacgctga atggcgtaac caaacccgtc acgctggaag cgaaattaat
    44401 tggtcagggc aacgacccat ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat
    44461 taagctcaag gacttcaata tcaagacaga tttaggtcca gcttctcagg aagtggatct
    44521 gattatttca gtggaaggcg tacagcagaa gtaagcgtga aatgttgccg gatggcacgc
    44581 atccggcaat gatatttatt ctgttggatc aggaatgccc agtgttgtat tcagacgtcc
    44641 acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc gtcgctgttc
    44701 ttctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt tgtatttttc
    44761 cgcgcatact ggacactgaa taaacagcag gtggcagcca tcatttttac agttggtatg
    44821 gctgtcgcac ggcgcgccgc actggtggca atgcgcgata atctcatccg atatacgttc
    44881 gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca agccctgctc
    44941 gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt tgttgaatcc
    45001 gttatgtttc atccaagcac tggccttttc acaacgaatg ccgccggtgc agtacatgac
    45061 gattttttta tctttatgtg cctgcatcat ctcgactgct tttggcagct gctcacggaa
    45121 ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc acttcatact ca
//
LOCUS       DAJTYD010000009        43879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTYD010000009 DAJTYD010000000
VERSION     DAJTYD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.009"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            83..607
                     /gene="lpfE"
                     /locus_tag="OO585_000497"
     CDS             83..607
                     /gene="lpfE"
                     /locus_tag="OO585_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCR6628192.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            860..2551
                     /gene="eptB"
                     /locus_tag="OO585_000498"
     CDS             860..2551
                     /gene="eptB"
                     /locus_tag="OO585_000498"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6628193.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2643..2719
                     /locus_tag="OO585_000499"
     tRNA            2643..2719
                     /locus_tag="OO585_000499"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2677..2679,aa:Pro,seq:cgg)
     gene            3463..5070
                     /gene="dppA"
                     /locus_tag="OO585_000500"
                     /pseudo
     CDS             3463..5070
                     /gene="dppA"
                     /locus_tag="OO585_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5378..6397
                     /gene="dppB"
                     /locus_tag="OO585_000501"
     CDS             5378..6397
                     /gene="dppB"
                     /locus_tag="OO585_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6628194.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6407..7309
                     /gene="dppC"
                     /locus_tag="OO585_000502"
     CDS             6407..7309
                     /gene="dppC"
                     /locus_tag="OO585_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6628195.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7320..8303
                     /gene="dppD"
                     /locus_tag="OO585_000503"
     CDS             7320..8303
                     /gene="dppD"
                     /locus_tag="OO585_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6628196.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8300..9304
                     /gene="dppF"
                     /locus_tag="OO585_000504"
     CDS             8300..9304
                     /gene="dppF"
                     /locus_tag="OO585_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6628197.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9334..10605)
                     /locus_tag="OO585_000505"
     CDS             complement(9334..10605)
                     /locus_tag="OO585_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6628198.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11081..11188
                     /locus_tag="OO585_000506"
     CDS             11081..11188
                     /locus_tag="OO585_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6628199.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11275..12954)
                     /gene="bcsG"
                     /locus_tag="OO585_000507"
     CDS             complement(11275..12954)
                     /gene="bcsG"
                     /locus_tag="OO585_000507"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6628200.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12951..13142)
                     /gene="bcsF"
                     /locus_tag="OO585_000508"
     CDS             complement(12951..13142)
                     /gene="bcsF"
                     /locus_tag="OO585_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6628201.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13139..14710)
                     /gene="bcsE"
                     /locus_tag="OO585_000509"
     CDS             complement(13139..14710)
                     /gene="bcsE"
                     /locus_tag="OO585_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6628202.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14983..15171
                     /locus_tag="OO585_000510"
     CDS             14983..15171
                     /locus_tag="OO585_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6628203.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15183..15935
                     /gene="bcsQ"
                     /locus_tag="OO585_000511"
     CDS             15183..15935
                     /gene="bcsQ"
                     /locus_tag="OO585_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6628204.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15932..18550
                     /gene="bcsA"
                     /locus_tag="OO585_000512"
     CDS             15932..18550
                     /gene="bcsA"
                     /locus_tag="OO585_000512"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6628205.1"
                     /translation="MSILTRWLLIPPVNARLIGGYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            18561..20899
                     /gene="bcsB"
                     /locus_tag="OO585_000513"
                     /pseudo
     CDS             18561..20899
                     /gene="bcsB"
                     /locus_tag="OO585_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            20906..22012
                     /gene="bcsZ"
                     /locus_tag="OO585_000514"
     CDS             20906..22012
                     /gene="bcsZ"
                     /locus_tag="OO585_000514"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6628206.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21994..25467
                     /gene="bcsC"
                     /locus_tag="OO585_000515"
                     /pseudo
     CDS             21994..25467
                     /gene="bcsC"
                     /locus_tag="OO585_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25549..27537
                     /gene="hmsP"
                     /locus_tag="OO585_000516"
     CDS             25549..27537
                     /gene="hmsP"
                     /locus_tag="OO585_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6628207.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27720..29006
                     /gene="dctA"
                     /locus_tag="OO585_000517"
     CDS             27720..29006
                     /gene="dctA"
                     /locus_tag="OO585_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6628208.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPVSVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29226..30722
                     /locus_tag="OO585_000518"
     CDS             29226..30722
                     /locus_tag="OO585_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6628209.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30818..31747)
                     /gene="kdgK"
                     /locus_tag="OO585_000519"
     CDS             complement(30818..31747)
                     /gene="kdgK"
                     /locus_tag="OO585_000519"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6628210.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31979..32746
                     /gene="pdeH"
                     /locus_tag="OO585_000520"
     CDS             31979..32746
                     /gene="pdeH"
                     /locus_tag="OO585_000520"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6628211.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32816..34876
                     /locus_tag="OO585_000521"
     CDS             32816..34876
                     /locus_tag="OO585_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6628212.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35110..36432)
                     /locus_tag="OO585_000522"
     CDS             complement(35110..36432)
                     /locus_tag="OO585_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6628213.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36843..37856)
                     /gene="yhjD"
                     /locus_tag="OO585_000523"
     CDS             complement(36843..37856)
                     /gene="yhjD"
                     /locus_tag="OO585_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6628214.1"
                     /translation="MTQENEIKRPTQDLELEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMLGK
                     TQP"
     gene            complement(37905..38804)
                     /locus_tag="OO585_000524"
     CDS             complement(37905..38804)
                     /locus_tag="OO585_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6628215.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39324..39926
                     /locus_tag="OO585_000525"
     CDS             39324..39926
                     /locus_tag="OO585_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6628216.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39977..41626)
                     /locus_tag="OO585_000526"
     CDS             complement(39977..41626)
                     /locus_tag="OO585_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6628217.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42030..43427
                     /gene="ccp"
                     /locus_tag="OO585_000527"
     CDS             42030..43427
                     /gene="ccp"
                     /locus_tag="OO585_000527"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6628218.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43638..>43879
                     /locus_tag="OO585_000528"
     CDS             43638..>43879
                     /locus_tag="OO585_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6628219.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10540 a        11001 c        11925 g        10413 t
ORIGIN      
        1 ccgttagcac taccggaaaa ttacccgctg ccggggatta tagcggtatt gctaccatac
       61 atgtcgaact ggagtaacag tcatgaagcg aacaatactt gcgttatgcc tgctgcctct
      121 ttccttttcg tcattcgcgg caaccacgga tttagggccg aaaggaacat tgaaattcac
      181 ccttaaaatc agccagggtg cttgcgaact ggtgaaagac agcgttgagg ttgacatggg
      241 caccgcggtt ctcaaaaagc cggtaagaat cggaacggaa attaatccca ctcctttttc
      301 catcggcctg aaaaactgta gcgaagtggt cagagcctac gtgactatgg acggtacacc
      361 caatgcagac gactcaaatc tttttgcgct ggatgcgggt ggcgctacag gcgttgggct
      421 gaaaatcaaa accgctgctg gagtacaaca gttcccggaa aatacaacgc cgacaccggt
      481 tgagtttgca attaacggtg atggcgcaca tcaactgaac tatatcgcca gctacgtgcc
      541 tattcgcgcc gatgcaacta ttggtcgcgc agatgcaacg gtcgacttta ctgtacagta
      601 cgaataattt cacatcttaa gggccagtta actggccctt tttactgctt tttgtaacgt
      661 taaagtctct tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc
      721 acactttttc gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact
      781 acctccgcac ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt
      841 ttgtccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt
      901 tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg
      961 cagctatgcg cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc
     1021 caccgtactg gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg
     1081 gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt
     1141 ccttaatgtg gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct
     1201 gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc
     1261 tcttatcctt atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc
     1321 agggcagcgt attcgcagcc tggcggtcgt cgtactggcg ggtattatgg tttgggcacc
     1381 gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc
     1441 gagttatggc ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg
     1501 gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc
     1561 gaagaagttt acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat
     1621 cggtgaaacc acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc
     1681 gaaactggcc caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac
     1741 caaactctca ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg
     1801 cacattaaaa gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct
     1861 ctacgctatg cagagcgaaa tgtggttcta cagcaacacg atggcggaca acattgctta
     1921 tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct
     1981 ggtagacgaa atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct
     2041 gcataccaaa ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg
     2101 gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta
     2161 tgacaactcg gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga
     2221 taagaaagcg attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca
     2281 cctgcacggc acgccgcgtg aattggcacc gccggagcag ttccgcgtac cgatgatggt
     2341 ctggatgtca gataaatatc tggaaaatcc ggccaatgcg caggcatttg cgcagctgaa
     2401 aaaagaagcc gacatgaaag tgccacgccg tcacgtagag ctgtacgata ccatcatggg
     2461 ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat
     2521 cccgcaggca aaagaggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa
     2581 aggcattttg ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca
     2641 ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg
     2701 aatcctctat caccgaccaa attcgaaaag cctgctcagc gagcaggctt ttttgcattt
     2761 gaagcccatg aggatgagaa cctccggggg ctgggaggtt cgactcgacg taggcctgat
     2821 aagacgcgcc agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac
     2881 cgccttattc gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact
     2941 atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt
     3001 tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg ttcatggtat
     3061 taatcgctca gatatccacc ttttcctcag atttttttca ttaaatgcat aaaacttaat
     3121 caccaaatgt tgctaaatat taagctgatt gttctggtta tgttggagta gcacaaattt
     3181 ctctgctgca aatagctgtt tgaagttttt taatctttgt ttgtgggttc tcaccgttgg
     3241 tgtaaatccc ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg
     3301 aggggcattt tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca
     3361 cctcataacg ttgacccgac cgggcaaaaa acaaaaaagg tcaggtagcg acaacccact
     3421 gcaaagggtt aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa
     3481 aagtcaggga tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt
     3541 caggctaaaa ctctggttta ttgctcagaa ggctctccgg aagggtttaa cccgcagctg
     3601 tttacctccg gcaccaccta tgacgcctct tccgtaccgc tttataaccg cctggttgaa
     3661 tttaaaatcg gcaccactga agtgataccg ggcctcgctg aaaagtggga agtcagctaa
     3721 gacggtaaaa tctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa
     3781 ttcaaaccga cgcgtgaact gaacgccgat gacgtggtgt tctcgttcga ccgtcagaaa
     3841 aacgcgcaaa acccgtacca taaagtctct ggcggcagct acgaatactt cgaaggcatg
     3901 ggcttgccgg agctgatcag tgaagtgaaa aaggtggacg acaacacagt tcagttcgta
     3961 ctgactcgcc cggaagcgcc gttcctcgct gatctggcaa tggacttcgc ctctattctg
     4021 tcaaaagaat atgctgatgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac
     4081 ccaatcggaa ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac
     4141 aaagcgtttg atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt
     4201 acccctgatg cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg
     4261 tacccgaacc cggcagatat cgctcgcatg aagcaggata aatccatcaa cctgatggaa
     4321 atgccggggc tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac
     4381 gtgaaagttc gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt
     4441 tatcagggcg cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac
     4501 gacgacgttc aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg
     4561 ggtctggaaa aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac
     4621 ccgaacgctc gctgcatggc ggagatgatt caggcagact gggcgaaagt gggcgtgcag
     4681 gccaaaatcg tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat
     4741 cagacggtaa tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc
     4801 ctgttcagct gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg
     4861 tttgaagatc tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg
     4921 tacaagcaat cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc
     4981 accgtgtttg aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa
     5041 catcacttcg aaaatgtctc tatcgaataa ttaaaagcca tacaagactg atggtaaagg
     5101 caaaactgcc tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt
     5161 tgcacgattt tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac
     5221 tttgtcaaca atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag
     5281 caatacttca ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc
     5341 agttccaggc tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg
     5401 tttgggactc gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca
     5461 catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg
     5521 tcacgcgcag ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca
     5581 ttacatttgg ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt
     5641 atgggaagag ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat
     5701 ttttgctacg gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat
     5761 tttcgatcac actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg
     5821 gggcatgatg ctgatcatgc tggtttcggt gcactggaac ctgacgcccg cctccggtcg
     5881 cgtgagcgat atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga
     5941 caccgccatc tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc
     6001 tgccattgtg ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat
     6061 gctggaagtg ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat
     6121 gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg ccggtggtga ccgttatcgg
     6181 cctgcaggtg ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc
     6241 cggtctggga cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg
     6301 cggcgtattg ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta
     6361 cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac
     6421 tgaaaataaa gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta
     6481 ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac gtcgtcatcg tgctgttcat
     6541 cgcgatcttt gccaactgga ttgcacccta taacccggcg gaacagttcc gcgatgcact
     6601 gctcgccccg ccagcctggc aggaaggcgg cagcatggcg cacttgctgg gcaccgatga
     6661 cgtaggccgt gatatgctgt cgcgcctgat gtacggtgcg cgcctgtcgc tgctggttgg
     6721 ctgtctggta gttgtgttat cgctgattat gggcgttatt ctcggcctga tcgccggtta
     6781 ctttggcggc ctagtcgata acatcattat gcgcgtggtc gatatcatgc tggcgctgcc
     6841 aagtctgctg ctggcgctgg tgctggtggc aattttcggc ccgtcgattg gtaacgccgc
     6901 gctggcactg accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct
     6961 ggtggaagtt aaccgcgatt acgtcaccgc gtctcgcgtg gcgggtgccg gggcgatgcg
     7021 tcagatgttt attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct
     7081 cggtttctct aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca
     7141 gccgccaacg ccggagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc
     7201 ctggtgggtc gtgaccttcc cgggtctggc gatcctgctg acggtgctgg catttaacct
     7261 gatgggtgac ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga
     7321 tggcgttatt aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc
     7381 gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga agtggttggg attgtgggtg
     7441 agtccggctc cggtaagtcg gtcagttccc tggcgattat ggggctgatt gattatccgg
     7501 gccgcgtaat ggcggaaaaa ctggtgttta acggtcagga tttgcagcgt atatcagaaa
     7561 aagagcgccg caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca
     7621 gccttaaccc gtgctacacc gtgggtttcc agatcatgga agcgattaag gtgcatcagg
     7681 gcggcaacaa aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc
     7741 ccgatccggc ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc
     7801 gcgtgatgat cgccatggcg attgcctgtc gaccaaaact gctgattgcc gatgagccga
     7861 ccaccgcgct ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc
     7921 agaaagagaa catggcgctg gtgttgatta cccatgacct ggcgctggtg gcggaagctg
     7981 cgcataaaat cattgtgatg tatgcaggcc aggtggtgga aacgggcgat gcgcacgcca
     8041 tcttccatgc gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg
     8101 ctcaggacaa agaacgtctg gcgtcgttgc caggtgtcgt tcccggcaag tacgaccgcc
     8161 cgaacggctg cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag
     8221 aaccggcgct gaatatgctc gctgacgggc gtcagtccaa atgccattac ccacttgatg
     8281 atgccgggag gccgacacta tgagtacgca agaggccacc ctgcaacaac cgctgttgca
     8341 ggctatcgac ctgaaaaaac attatccggt gaagaaaggc atgttcgcgc cggaacgtct
     8401 ggttaaagcg ctggatggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt
     8461 gggcgaatct ggctgcggta aatcgaccct cggtcggttg ctgacgatga ttgaaacgcc
     8521 caccggtggc gagctgtatt accaggggca ggatctgctt aagcacgatc cgcaggcgca
     8581 gaagctgcgt cggcagaaaa tccagatcgt cttccagaac ccttacggtt cgctgaatcc
     8641 gcgtaaaaaa gtcgggcaaa ttcttgaaga gccgctgctg atcaacacca gcttaagcaa
     8701 agaacagcgt cgggaaaaag ccctgtcgat gatggcgaaa gtcggcctga aaaccgagca
     8761 ctatgaccgc tatccgcata tgttctccgg cggtcagcgt cagcgtatcg ccatcgcccg
     8821 tggtctgatg ctcgacccgg atgtggtgat tgccgatgaa ccggtttccg cgctggatgt
     8881 ttcagtgcgc gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctgtc
     8941 ttatgtcttt atctcccacg acctgtcggt ggtggagcac attgctgatg aagtgatggt
     9001 gatgtacctg ggccgctgcg tggagaaggg aacgaaagac caaatcttca ataacccgcg
     9061 ccatccgtac actcaggcgc tactttccgc gacgccgcgc ctgaacccgg acgatcgccg
     9121 cgagcgcatc aagctcagcg gtgaactacc aagcccactg aatccaccgc cgggttgcgc
     9181 cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa
     9241 agactacggc ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg
     9301 ttaactcaca aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga
     9361 caagcatagc aaaatgccgt aaagcagaat tagccaggct ttaaatccgc gaagtttttc
     9421 cagttgtgcg actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa
     9481 cgggcttccc aactgaaaga acaccagcac cgatacacga aacgaaaccc aaatcgtcaa
     9541 cgtaatgacg ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt
     9601 atcaataatt cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag
     9661 ataaatgcca aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg
     9721 aatgatatgt ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa
     9781 ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat
     9841 caccgcgatg agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag
     9901 tactttatct gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa
     9961 taccgcagaa aagaagcaaa atggaattgt aagcaggaca tcgcggaaaa agacggaggc
    10021 ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac
    10081 gacaataatc cctactttga ccaccaccat tggcccggaa atcttaaaca gtaatcgttc
    10141 accaccagac gcaatcgcta ccagtacggc gaaaatagcg actttataaa gtagagattg
    10201 tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac
    10261 ggagagagag tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa
    10321 aattccccag ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc
    10381 gctttcagaa agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat
    10441 gatcgctgcg gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac
    10501 ggttccggca ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt
    10561 aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga
    10621 aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag
    10681 tcgaatacgc agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc
    10741 gcaattctac aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc
    10801 aactattgca aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg
    10861 tgctgacagt aaaaagcaat cgtaaaatca gcggcaagcc gaaagggttc cggtgaggcg
    10921 caatgttgcg ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca
    10981 cgttgcaggt atgcacctga caacatcacg ggggctaatc ttgaacccaa acaactcaag
    11041 aatagccgcg aaacgttggc attacaacac aggcggctat atgacgctcg cagaactggg
    11101 catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat
    11161 cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg
    11221 caatttgcgc ccggatcagg ccgcagaggt taaactctgc ggcctttttc gtgcttactg
    11281 cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac ggtttatcct ggtattgaat
    11341 aactactgca tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt
    11401 tttccagtca acattgtctt cggtgaaaat cttgccatca agaacgcgaa ccaccagatc
    11461 ggagatagcc aggaagctgc tcggttggtc gatgacaatc ggtgccccct gatgtggtgc
    11521 cttcatgccg aagaatttca ccccaacggg gacgtctgtg atagacgggc tagggatatc
    11581 acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac
    11641 gaccaccatc actttacgac ccgatttctc cagttcagta aagaaggcgt ccagttcatc
    11701 aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt
    11761 gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc
    11821 ggtaacgtcc agccagcggt taagcacggt ggtatcgtca taaaccggcg aaccatcaaa
    11881 gcccagcaaa ataaccggca gatttgtttg atccatcaat tcagtctgca tgccgccatt
    11941 ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg
    12001 ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata
    12061 cagattagtg tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc
    12121 actgtaggag gtggcggagt taaagttctt gaactcaata tcgaaatgcg accacagtgg
    12181 atgcgacatc aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac
    12241 cagtagttca aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt
    12301 cgcctccgcg ttatagaaat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgt
    12361 agtttgtgcg ggcatatcac ccactaccgg tgcgccaccc gtcgtctcaa cggttgccgc
    12421 tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc acaaggagaa
    12481 acttggtccc gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt
    12541 aatgcgaatc cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat
    12601 catctgccag ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc
    12661 cacctgcgaa ccctggctca ttatgctttc cgggccaggc aaccaggtgt catgccagaa
    12721 caaagcaaag ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtagcgcgg
    12781 aatgggcatc agcagaaacg cggcaaacac caaattgagg agcggatgga agttaagata
    12841 tcccgcccac aacaggccga acttaaccag aaaatagaag ttccagccgg aaaggccgcg
    12901 ccagtattgc cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg
    12961 gttgccctgg ctttttccgt gcggcgtaat gtcccggccg gtttaacata acgaggttta
    13021 gcaaagaaca aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc
    13081 agcgggaaaa atatcagtgc gcaaacgaca ataatttcaa tgatatcgct gatggtcatc
    13141 atgatgagcg ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt
    13201 ggcgaccatc gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca
    13261 tgccccattg ttctggggca agcaggcgca tctgcaccag ctcggcactg atttgatcat
    13321 cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat
    13381 tcaacgcggt atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac
    13441 cgccaatggt catgatatcg ccggtacggt tagggcgaca cagcgtcagg gcttgttcaa
    13501 cgcggatacc cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggtaata
    13561 gagggttatt catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac
    13621 gcagtttgag cggctgggtc attgacagca aggtagtgat atcttccggc acatagcgac
    13681 taaacttctg cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc
    13741 acggaataac catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc
    13801 tagcggtatt ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat
    13861 gaatgctgcg ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg
    13921 ccgcctgagc ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt
    13981 gttccgatag cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt
    14041 cgctgcgtgg ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt
    14101 gctgaacgct aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat
    14161 ccagcaaatg ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt
    14221 cctcaagcaa caatgaaaat tgtttatcgt tattatttcc gggattaatt accaaaagcg
    14281 aacaatgggt taacctgctc catttattca ttttatccaa ccatgagcga agccgctcgg
    14341 caggaatgtt ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat
    14401 caatagaaca ctgcaaatca cgggtcaaat agtatagacc tttttcatga tttagcattg
    14461 aaaataattt tattttttcc ggcccttgat aatcatctaa ttgaaagatt ttcgccggat
    14521 cgctgtccat gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca attgtttgat
    14581 tcgccagact gatagcatct tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg
    14641 gcatatgtcg cagctcatcc cataatgatg agataccgat agagaataca gggcccacaa
    14701 tgtccctcat gtcaccattt tgtctgatta tcagtttact agcgaaagct aagaaataaa
    14761 cctaccattg aaattaaaac ttatcagatt tagcatgtga atcaatttag aaggtcacaa
    14821 gacgtgtgat tttagtcttt tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa
    14881 agtgagcaaa ttcccgtctc gccgtaaaca ataaaccggc gaaatgctca cggttagaat
    14941 tgtctcatga acggtacggt tatttcatag ggatcaagca aaatgaataa caatgaacca
    15001 gatactctgc ctgatcccgc gataggctat atcttccaga atgatattgt ggcgttaaag
    15061 caggcatttt cactgcctga tattgattat gccgatattt cccaacgcga acagttggcc
    15121 gcggcattaa aacgctggcc gttgctggca gagtttgcgc aacaaaagta ggggattggt
    15181 gaatggccgt actgggattg cagggggtgc ggggaggcgt ggggacaaca accatcaccg
    15241 ccgcattagc ctggtcatta caaatgttgg gagaaaatgt cctggtggtc gatgcctgcc
    15301 cggacaactt gttgcgcctg tcatttaatg ttgattttac ccaccgtcag ggctgggcca
    15361 gagcgatgct ggatggccag gactggcgtg acgctgggtt gcgctacacc tcgcagctcg
    15421 atttgctgcc ttttggtcag ttatccattg aagaacaaga aaatccacag cacaggcaaa
    15481 cccggctgag cgatatttgc tccggcttac agcaactaaa agccagcggg cgttaccagt
    15541 ggattttaat cgacttaccg cgtgatgcct cgcagataac ccaccagctg ctgagtttgt
    15601 gcgatcactc gctggcaatc gtcaatgtgg atgccaactg ccatatccga ctgcatcagc
    15661 aagcgctgcc ggatggcgca catattttga ttaatgactt ccgtattggc agtcaggttc
    15721 aggacgatat ttaccagctt tggttgcaaa gccagcgccg attactgccg atgctcattc
    15781 atcgtgatga agcgatggct gaatgcctgg cggctaagca accagtaggt gaatatcgca
    15841 gtgatgcgct ggcggctgaa gagatactga cgctggcgaa ctggtgcctg ttgaactact
    15901 ccgggctgaa aacgccagtc gggagtgcat catgagtatc ctgacccggt ggttgcttat
    15961 cccgccggtc aacgcgcggc ttatcggggg ttatcgcgat tatcgtcgtc acggtgcgtc
    16021 ggctttcagc gcgacgctcg gctgtttctg gatgatcctg gcctggattt ttattccgct
    16081 ggagcacccg cgctggcagc gtattcgcgc agaacataaa aacctgtatc cgcatatcaa
    16141 cgcctcgcgt ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttact
    16201 gatcggtaca tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt caggtctgca
    16261 gaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa
    16321 aacacagcat cttgatgaga aaaaagagct cggtcatttg agtgccgggg cgcggcggtt
    16381 gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcaacc
    16441 gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggtgtag cgctgatcgt
    16501 acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc
    16561 ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct
    16621 ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg
    16681 ctacttccag gtcgtatggc cgctgaatcg tcagccggtg ccattgccga aagatatgtc
    16741 gctgtggccg tcggtggata tctttgtccc gacttacaac gaagatctca acgtggtgaa
    16801 aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga acatctggat
    16861 cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata
    16921 tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg cgctgaaata
    16981 tgccaaaggc gagttcgtat cgattttcga ctgcgaccac gtaccaacgc gatcgttcct
    17041 gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc agacgccgca
    17101 ccacttcttc tcgccggacc cgtttgaacg caacctgggg cgttttcgta aaacgccgaa
    17161 cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt
    17221 cttctgcggt tcctgtgcag taattcgtcg caaaccattg gatgaaattg gcggtattgc
    17281 tgtcgaaacg gtaaccgaag atgcgcacac gtctctgcgc ctgcaccgtc gtggctacac
    17341 ctctgcatat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca
    17401 tatcggtcag cgtattcgct gggcacgcgg gatggtgcaa atcttccgtc tcgataaccc
    17461 gcttaccggt aaagggctga aattcgccca gcggctgtgc tacgtcaacg ccatgttcca
    17521 cttcttgtcg ggcattccac gactgatctt cctgactgcg ccgctggcgt tcctgctgct
    17581 tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctgttcgtgc tgccgcatat
    17641 gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact ctttctggag
    17701 tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat
    17761 taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta
    17821 cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctgctcaacc tggtgggtgt
    17881 tgcggtcggc atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt
    17941 cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg cggtatcggt
    18001 agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc
    18061 ccgcgaagat ggtcacctct tttcgtgtac cgttcaggat ttctccgacg gtggtttggg
    18121 gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa
    18181 acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt
    18241 tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt
    18301 tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga
    18361 aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc
    18421 gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc
    18481 gtttattctg cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc
    18541 tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca gtggctatgg
    18601 ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca
    18661 atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg caagtgatgc
    18721 cgggcgtgca gggcgctgat gcgccagtcg tggcgcagaa cggtccttcg cgtgatgtga
    18781 agctgacctt tgcgcaaatt gcgcctccgc cgggcagcat ggtgctacgt ggcattaacc
    18841 cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag gcgatgctca
    18901 acctcgaata catgccatcg ccatcgttac tgcctgtcca gtcgcagtta aggtttatct
    18961 caatgatgag ctgatgggcg tactgccagt gaccaaagaa cagttgggta aaaaaacgct
    19021 ggcgcaaatg cccattaacc cactgtttat taccgacttc aaccgtgtgc ggctggagtt
    19081 tgtcggccat tatcaggacg tgtgcgaaaa cccggccaga accacgcttt ggctggatgt
    19141 tgggcggagc agtggactgg atctgaccta tcagaccctg aatgtgaaga atgacctgtc
    19201 acacttcccg gtgccattct ttgacccgcg cgataaccgt actaacacct tgccgatggt
    19261 ctttgcgggt gcgccggatg ttgggctgca acaagcatct gccattgtcg cctcgtggtt
    19321 tggttcgcgt tctggctggc gtgggcagaa cttcccggtg ctctataacc aactgccgga
    19381 tcgtaatgcc attgtctttg caaccaacga caaacggccg gacttcctgc gcgatcatcc
    19441 ggcggtaaaa gccccggtga ttgagatgat caaccatccg cagaatcctt acgtcaaact
    19501 gctggtggtg tttggtcgtg acgacaaaga cctgttgcag gcagcgaaag gtatcgctca
    19561 gggtaacatt ctgttccgtg gtgaaagcgt ggtagtgaat gaagtgaaac cgctgctacc
    19621 gcgtaagccg tacgatgcgc cgaactgggt acgtaccgat cgtccggtca catttggcga
    19681 actgaaaacc tatgaagaac agttacaatc cagcggtctt gagccagcag cgattaacgt
    19741 ttcgctaaac ctgccgccgg atctctacct gatgcgcagt accggcattg atatggatat
    19801 taattaccgc tacaccatgc cgccggtgaa agacagttcg cggatggata tcagcctgaa
    19861 taaccagttc ctgcaatcct tcaacctgag cagcaaacag gaggcgaacc gcctgctgct
    19921 gcggattccg gtattacaag gtttgctgga tggcaaaaca gatgtctcta ttccggcgct
    19981 gaaactgggc gcgaccaacc agctgcgctt cgactttgag tatatgaacc cgatgccggg
    20041 cggttcggtg gataactgta ttaccttcca gccggtgcag aatcatgtgg tgattggtga
    20101 cgactccacc atcgacttct cgaagtatta ccacttcatc ccgatgccgg atctacgcgc
    20161 ctttgctaac gcgggcttcc cattcagccg gatggcggat ctgtcgcaaa ccatcaccgt
    20221 gatgccgaaa gcgcctaacg aagcacagat ggaaacgttg ctgaatactg ttggttttat
    20281 cggcgcacag acgggcttcc cggcgattaa tctgacggtg accgatgatg gcagcaccat
    20341 tcagggcaaa gatgccgaca tcatgatcat cggtggtatc ccggacaaac tgaaagacga
    20401 taagcagatc gacctattgg tgcaggcgac cgaaagctgg gtgaaaacac cgatgcgcca
    20461 gaccccgttc cccggcattg tgccggacga gagcgatcgc gcggcagaaa cccggtcaac
    20521 gctgacctct tccggtgcga tggcggcggt gattggcttc cagtcgccgt ataacgacca
    20581 gcgcagcgtg attgcgctgt tggcagatag cccacgcggt tatgaaatgc ttaacgatgc
    20641 ggtgaacgat agcggcaaac gcgccaccat gttcggttcg gtcgcggtga tccgcgagtc
    20701 cggtatcaac agcctacgtg ttggcgacgt ttattacgta ggtcatctgc cgtggttcga
    20761 gcgcgtgtgg tatgcgctgg caaaccatcc gattctgctg gcggtgctgg cggctatcag
    20821 tgtgatattg ctggcatggg tactgtggcg tctgctgcga attattagtc gtcgtcgtct
    20881 taacccggat aacgagtaat tgaagatgaa tgtgttgcgt agtggaatcg tgacgatgct
    20941 gttgttggct gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa
    21001 aaaggattac atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac
    21061 cacctccgaa gggcaaagtt acggtatgtt ctttgccctg gcggctaacg accgtgcagc
    21121 tttcgataat attctcgact ggacgcagaa caatctcgct cagggttctt taaaagaacg
    21181 tttgcccgcc tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa
    21241 ttcggcctcc gatggtgatg tctggatggc ctggtcgttg ctggaggcgg ggcgtttgtg
    21301 gaaagagcag cgttataccg acatcggcag cgcgttgcta aaacgtatcg cgcgggagga
    21361 agtggtgacg gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga
    21421 ggataacagc tggcgtttta accccagcta cctgccgccg acgctggcgc agtatttcac
    21481 ccgctttggc gcgccgtgga ctacgctgcg cgaaaccaat caacgtttat tgctggaaac
    21541 cgccccgaaa ggcttttcgc cagactgggt gcgttatgag aaagacaaag gctggcagct
    21601 aaaagccgaa aaaacattga tcagcagcta cgacgccatc cgcgtttaca tgtgggtagg
    21661 catgatgcct gacagcgatc cgcagaaagc gcggatgctc aaccggttta aaccgatggc
    21721 gacattcact gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc
    21781 gcagggtaaa ggaccagtcg gtttttctgc cgccatgctg ccctttttac aaaaccgcga
    21841 tgcgcaggcc gttcagcgtc agcgcgtggc cgataacttt cccggcagcg atgcctatta
    21901 caactatgtg ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac
    21961 aaaaggtgag ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc
    22021 acgctttccc tcggtctggc cgtcatgccg atggtcgagg cagcaccaac cgctcagcaa
    22081 cagttgctgg agcaagttcg gttaggcgaa gcgacccatc gtgaagatct ggtgcaacag
    22141 tcgttatatc ggctggaact tattgatccg aataacccgg acgtcgttgc cgcccgtttc
    22201 cgttctttgt tacgttaggg cgatattgat ggcgcgcaaa aacagctcga tcggctgtcg
    22261 cagttagcgc cgagttcaaa tgcgtataaa tcgtcgcgga ctacgatgct actttccacg
    22321 ccggatggtc gtcaggcact gcaacaggca cgattgcagg cgacgaccgg tcatgcagaa
    22381 gaagctgtgg cgagttacaa caaactgttc aacggtgcgc cgccggaagg tgacattgct
    22441 gtcgagtact ggagtacggt ggcgaaaatt ccggctcgcc gtggcgaagc gattaatcag
    22501 ttaaaacgca tcaatgcgga tgcaccgggc aatacgggcc tgcaaaacaa tctggcgcta
    22561 ttgctgttta gtagcgatcg ccgtgacgaa ggttttgccg tcctggaaca gatggcaaaa
    22621 tcgaacgccg ggcgcgaagg ggcctctaaa atctggtacg ggcagattaa agacatgccc
    22681 gtcagtgatg ccagtgtgtc ggcgctgaaa aaatatctct cgatctttag tgatggcgat
    22741 agcgtggcgg ctgcgcaatc gcaactggca gaacagcaaa aacagctggc cgatcctgct
    22801 ttccgcgctc gtgcgcaagg tttagcggcg gtggactctg gtatggcggg taaagccatt
    22861 cccgaactac aacaggcggt gcgggcgaac ccgaaagaca gtgaagctct gggggcgctg
    22921 ggccaggcgt attctcagaa aggcgatcgc gccaatgcag tggcgaatct ggaaaaagcc
    22981 ctcgcactgg acccgcacag cagcaacaac gacaaatgga acagtctgct gaaagtaaac
    23041 cgctactggc tggcgatcca gcagggcgat gctgcgctga aagccaataa tcctgaccgg
    23101 gcagaacgcc tgttccagca ggcgcgtaat gtcgataaca ccgacagtta tgcagtgctg
    23161 gggctgggcg atgtggcgat ggcgcgaaaa gattatcccg ccgccgaacg ttattatcag
    23221 cagaccttgc gtatggacag cggcaacact aacgccgtgc gcgggctggc aaatatttac
    23281 cgccagcaat cgccagaaaa agctgaagcg tttatcgcct cgctctctgc cagtcagcgg
    23341 cgtagcattg atgatatcga acgcagcctg caaaacgacc gtctggcaca gcaggcagag
    23401 gcactggaaa accagggcaa atgggcgccg gcggcagcac ttcagcggca acgactggcg
    23461 ctggaccccg gcagcgtatg gattacttac cgactttcgc aggatctctg gcaggccgga
    23521 caacgcagcc aggccgatac gttaatgcgc aatctggcgc agcagaagtc gaacgacccg
    23581 gagcaggttt acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg
    23641 gcgcatatca acagcctgcc gcgagcgcag tggaacagca atattcagga gctggttaat
    23701 cgactgcaaa gcgatcaggt gctggaaacc gctaatcgcc tgcgagaaag cggcaaagaa
    23761 gctgaagcgg aagcgatgct gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg
    23821 gctgactggg cgcagcagcg acgtgattac accgccgccc gcgctgcata tcagaatgtc
    23881 ctgacgctgg agccaactaa cgccgatgcc attctggggc tgacggaagt ggatattgct
    23941 gccggtgaca cagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc
    24001 tcgctgaaca cacagcggcg cgtggcgctg gcgcaggcgc agcttggaga taccgcagcg
    24061 gcgcagcaga cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa
    24121 agcgcgatgg tgctgcgcga cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag
    24181 gcgctggaaa cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag
    24241 gataacgaca cattcacccg actgacccgt aacgacgaga aagatgactg gctgaaacgc
    24301 ggcgtgcgca gcgatgcggc gggcctctat cgccagcagg atcttaacgt caccctcgag
    24361 cacgattact ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc
    24421 atgttgcagg tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc
    24481 aatatgaacg tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc
    24541 acctgtacat tacaggactg tagtggcaac cgcagccagt cggactccgg tgccagcgtg
    24601 gaggtcggct ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc
    24661 aacgtggtgg atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac
    24721 accgttaacg cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa
    24781 gactccccga gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta
    24841 agtctgagct acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag
    24901 ttaaccggta aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat
    24961 aaggtcatta accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat
    25021 tacgacaaag atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa
    25081 tacctgtcgt ttgctatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag
    25141 ctgggggcgt ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg
    25201 atgaatctga tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc
    25261 agcagtcagg gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac
    25321 tggtttgttg gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc
    25381 ctgctctacg tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag
    25441 ccgctgatac cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc
    25501 gatcgggtat actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt
    25561 cgctcgttaa caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt
    25621 attttttgca ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg
    25681 caactggaaa gcattgctcg ctctgtacgc gaacccttat cttcagctat tttgaaaggc
    25741 gatattcccg aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt
    25801 gccgatgtag tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc
    25861 ccggtgccgg taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt
    25921 tactcgctcg aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat
    25981 tccttccgta tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta
    26041 cttttgtcgc ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat
    26101 ccgttacgca atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac
    26161 caactggcat taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac
    26221 aacctcaacc agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc
    26281 ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc
    26341 gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg
    26401 ggcgtgctga aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa
    26461 tcggtactgt cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt
    26521 gccaacggtg tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcactatc
    26581 atgagcgagc gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg
    26641 gcgatgttct acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca
    26701 tttaccgctc gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa
    26761 gccgcccaga agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag
    26821 tttgctattt ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa
    26881 gtgttactgc gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat
    26941 cgcattgagt gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt
    27001 cgattgcttg cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct
    27061 gcgctgcaac tgatgcaccc aaatatggtg gcggatatgc tggaactgtt aacccgctat
    27121 cgcattcaac cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct
    27181 catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat
    27241 gatttcggca tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc
    27301 gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt
    27361 gctgcaatta tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag
    27421 actgaagcac aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt
    27481 tttgctcgcc cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta
    27541 ccccaaactg attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt
    27601 taaccttttt gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt
    27661 ttaaggccgc aggtacctca taaccttaca agacctgtag ttttactaaa ggacacccta
    27721 tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta
    27781 ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct
    27841 tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca
    27901 ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact
    27961 ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc
    28021 ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg
    28081 ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggtgagcg
    28141 tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg
    28201 gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt
    28261 tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt
    28321 tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc
    28381 agctgattat ctgtttctat attacctgta tcctgtttgt ggtgctggta ttgggttcaa
    28441 tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc
    28501 tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg
    28561 agaaactcgg ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta
    28621 accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta
    28681 acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta
    28741 aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg
    28801 tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag
    28861 aagctcgtgc gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt
    28921 gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg
    28981 gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag
    29041 tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg
    29101 tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta
    29161 acgtcattta gactgactga caacgggcag ttttttatta ccaggatagt tgatcagggg
    29221 ttcacatgca gggcacaaaa attcgacttt tagcgggcgg tttgctgatg atggccactg
    29281 ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt
    29341 ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa
    29401 ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc
    29461 acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt
    29521 cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg
    29581 acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc
    29641 tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg
    29701 cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc
    29761 gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac
    29821 ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga
    29881 cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt
    29941 ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc
    30001 gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt
    30061 gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg
    30121 acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtaacagca
    30181 aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca
    30241 acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg
    30301 cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga
    30361 aactggagct gcagaaactg tttgccgcct atgcgcgggc tgataccgat attctgatgg
    30421 gtcagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga
    30481 aactgcggca ggatttcctt aacagcctga cggtggagat gttaaatcag gatctgcgtc
    30541 agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta
    30601 acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatcgact gctgctgccg
    30661 ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt
    30721 aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag
    30781 gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg
    30841 ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg
    30901 cgttttccgc actgccgccc gtcaaacgta ccgccagata accggcgctg aaagagtcgc
    30961 cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga acatccacta
    31021 acccttcgcc agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt
    31081 tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg
    31141 cgtcttcatc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgttggtaca
    31201 cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca
    31261 cttttccgcc gttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg
    31321 ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt
    31381 cgcaaatcgc cgtagactgc tcactctcca gccagaattt ggcggcggct tcgttccgcc
    31441 agtagtagaa cgtacgctcg ccggtgctgt cggtttcaat gtagtaaagg cccggcagac
    31501 ggttttccat ccgttgggtc agggaaatat caacgttctc gccgtgccag gcgtccagca
    31561 tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag
    31621 gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct
    31681 taacgtccgc gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt
    31741 tggacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt
    31801 gctggtcaac aatattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga
    31861 aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttac
    31921 gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat
    31981 gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga
    32041 acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg
    32101 cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca
    32161 acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt
    32221 gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttatagagc acggcctgct
    32281 ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct
    32341 gcgccaaatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata tccgtctgcc
    32401 gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg gtgattttgg
    32461 taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc
    32521 gcgagaactg tttgtgatgc tgcgtcagtc gccggaagga cgcacactct tttctcagct
    32581 tttacatcta atgaatcgct attgtcgcgg ggtgattgtt gagggcgtag aaacgccgga
    32641 agagtggcgt gatgtacaga actcgcccgc attcgccgca caaggctggt ttctttcacg
    32701 cccggcaccg atagaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc
    32761 cgcctggctg gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag
    32821 caaggcaggc aaaataactg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc
    32881 gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt
    32941 ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg
    33001 gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga
    33061 catcattctt ggcaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt
    33121 agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggatcaa
    33181 gctcgaaaag cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt
    33241 taatctcgcc aacgatgata acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg
    33301 gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc
    33361 ggatctggaa atttttgttg atccgttagg caagccgctg ccgttcagcg aagttactgg
    33421 atcgaaaggt aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca
    33481 gggacgttat aacggtgaac agctcacggg tacgggaaaa ataggcggta tgctggcgct
    33541 gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt
    33601 tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa
    33661 attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac
    33721 cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt
    33781 ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt
    33841 ctacaccacc ggaaagccgc gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg
    33901 gctggcggac ctgggaccgt tgattggcgt tgattcgggg aaaggggcag aaaagtcgaa
    33961 acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta
    34021 tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaaggccg
    34081 acgtatcgag catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa
    34141 aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcggc
    34201 gaatatccat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg
    34261 tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga
    34321 aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa
    34381 cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga
    34441 gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt
    34501 gcgggtgaac tgcgcggcgg cgaatctgaa tattgccaac ggcgtagcgc gcccgcagat
    34561 ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc
    34621 ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg
    34681 ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc
    34741 gctgattgcc cgtggtgccg ttgcggcggc actggcaacg ctggtaacac cggcggcggc
    34801 gttattggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc
    34861 gcagatgaag aagtgatgag gttggattgt gtttgctggt gagttcggga gcagcgtgtt
    34921 cccctcaccc taatcctctc cccaaagggg cgaggagact gttttgtgcg tgttgaaaca
    34981 acgagtaccg cttttccgtt cactttctgg gggagggctg ttatgttcgc gatataacat
    35041 ctcgctccgc ttttctccct ctccctttca gggagagggc tggggtgagg gtggctgccc
    35101 acacgatact tacaacgact gatgttgcgt ctcatgggtc agcagcaggg cgattaacgt
    35161 caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt tagtctgcaa
    35221 ccaggctgcg atatacggcg caacggaagc cccgagaatc gacgatacgt tgtaagagaa
    35281 cgatgctccg gtgtaacgca cttctgtcgg gaacagctct ggtaacagcg cgcccatcgg
    35341 accgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa ccagaatcgg
    35401 gttgccagaa ccgagcaaag ggttaaaggc gaatagcgcg aacaggatga gcagcgtggt
    35461 gatgattacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccag cgactggcac
    35521 catcacgcca aaaccaataa ctgccatcat caacatccac aacacttcgt tacgcggcag
    35581 gccaagccca actggcgcgg cggcggtact aaaggtcatg gagtagaccg tcatgatgta
    35641 aaacagcgta taggttgcca gcataatgaa cgtacccagt acggttacgc gaacatgttt
    35701 ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga ctttctcaaa
    35761 caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca gcaccgccga
    35821 gaagataaat ggcacacgcc agccccagct cataaactgc tcgtcggtca gtagccagga
    35881 aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccca gctgcgggaa
    35941 ggagccgtac aatgcacgtt tgcgcggtgg ggcgttttca gtcgccagca gcgccgcgcc
    36001 gccccattca ccacctaagc ccagaccctg accaaatcga gccagcgcca gcagcagcgg
    36061 ggcgaaaata ccaatcgtgg catagcctgg cagcagacca atcaccacgg tcgaaatccc
    36121 catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc caaaatgacc
    36181 aaaaacggca gagccaatgg ggcgcgcaac gaaggcgatg gcgaaggtgg cgagcgactg
    36241 tagcgttgct gccgtaggat cgccctgcgg gaagaagata tgcggaaaca caataacggc
    36301 cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggct gtgccaatga gagaggcgac
    36361 aaggacttta ttacgcgagt tgatcggcgt gtattcttgc tcgtggtcga gtgttgtggc
    36421 tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt acgcacagca
    36481 aaagcgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg caaaaagggg
    36541 ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt tataaatagt
    36601 ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg ctggggttat
    36661 gagttagaat gaaattgttt gctcccaaat agatggctga agaggctctg gggacgagtt
    36721 tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta tccggcctac
    36781 ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc atccggccta
    36841 ttttaaggct gcgttttccc tagcattcgc gggtcgtctt tatattcggc ggtggcaatc
    36901 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    36961 acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt gtaggtcatc
    37021 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    37081 ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag atagttggcg
    37141 aagatggaaa tcgccaggcc aatcaatcgc cacgtcggtt tcagccactc aatgctgttc
    37201 aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    37261 gtcacaatca gcgcaatcaa caaaccaatc agtgaaataa aatcacgcag atattttacc
    37321 cagaacttct cctgatcttg cggcgagcgt tcccagacat cacgcgactg ggcacgaatc
    37381 gcttcacgca ggttacccat ccagttgatg ccggaataaa gtgccaccgc caggccgaca
    37441 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtggcggct
    37501 agtgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    37561 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    37621 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    37681 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    37741 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    37801 atcggctcga gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    37861 tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc caggtgctct
    37921 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    37981 tgcgggtaaa ccagcgaaag ggagagcggc tcggcacggt agccaggtaa tacttcaata
    38041 agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    38101 ccggcaagac cggcggcgag atagttttcg ctactattta ccgtcaacat gccgccagac
    38161 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    38221 tgcggtgtat aacgcactat tgcgtgtgaa gtcagatcat cggggctttg cggataacca
    38281 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    38341 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    38401 agaatatcta ccggacggtc atggctactc agttccagct caattcccgg atgcagatag
    38461 agaaattccg acaggcgcgg cagtaacagg tttttcgcga ttcctggcgg gatgtcgatg
    38521 cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag ttcgttgaga
    38581 ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt gagtttgacc
    38641 cgtcgcgtgg tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    38701 cgcgaaacac ttccctttgg caaagcaaag aaatccgctg cgcgggaaaa actttccagc
    38761 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    38821 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc taacatctaa
    38881 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    38941 aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag tgattccctt
    39001 taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    39061 attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    39121 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    39181 gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg gccaccgcca
    39241 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    39301 catattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    39361 ttcatggaat gaaaatcagt ttacagcagc gtattccagg ggtgagtatt cagggggcca
    39421 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    39481 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcagtttc
    39541 ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta caggaagtta
    39601 tccattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    39661 cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg acgacgccgg
    39721 aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt ttaaccatgt
    39781 tagcggcggg cgagtcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    39841 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    39901 caatgatgtt aaatatctcc tcctgaagag gataatttta cagccagcag gaagttagcg
    39961 gctggctgta aaaatattat ggttcgccgt acaaaccaat taaacggcgt accacaccgt
    40021 tagtccagcc aaacccatcc tgcaacggat actcgccacc gccgccttcg cgtggaacac
    40081 catcggcaat atggtatttt tcgatcagtt tgtgctgttc cagatagaac tgattcaccg
    40141 tcttcagcca gcttcgcgcg atttcatcac ccagaaggtc atcgccgtac attttaaatc
    40201 cctgaatcgc catccattgt aacggtgccc agccgttggg tttatcccac tgttcaccgg
    40261 tttcgtactc gcttgccaga atcccgccag gtgtcagtaa ccgactgcgc acggcatttg
    40321 ccagacgatc ggcctgttca tggttcgcca taccgacata gagtggcaca atggcggcag
    40381 cggaaaacag cgccagttgt tcgcgtcgcc agtcgtaatc gcggtagatg ccgttttcat
    40441 catcccagag gtaacgcttt accgcatcgc gacgggcact ggccttctgg cggaacagtg
    40501 cttctgtctc tttctcgcct ttcagcgccg agatgttggc gatggcgctc tccagtttaa
    40561 acaggaaggc attcagatcg atggggatga actgggtggt acgaatactc gccagacgac
    40621 cagtatcacg cagccaacgg gaagagtaat cccaaccgga ggccgccccc gctcgtaaat
    40681 cgcggtacac ctcgttgggc gggcgaccag aatgtttcgc ggtttcaacg tcctcaagcc
    40741 aggattcgtc acgcggcgtg tcgcgatcgt cccagtaacg gttgagcagc gatccgtccg
    40801 gcatccgcac aacatggcga taggcctgat tagggatcaa cgattctgca ccgtccatcc
    40861 agaaggcata ttccattttc aggtggtcga gatagcggcg cgcaccgcgt acaccatctt
    40921 cttcaaacaa ctccaccatc agcgcaaaaa ccggtggttg cgagcggctc aaataatagg
    40981 tgcggttgcc gttggggatg tgaccgtagt tttcgatcat ccaggcgaag ttatcggcca
    41041 tgcatttcag caaatcttcc cgaccacttt ccgccagccc cagcatggtg aaataggaat
    41101 cccaatagta ggtttcgcta aaacggccgc ccgggacaat atatgactgc ggtagcgcca
    41161 gcagagaaga ccacggaatg tgatcctgtg gttcgcgggt tagcaccggc cacagctggt
    41221 cgatatgctc tttcagggaa ttttgcgggt ccgatacata ctcgctggag tagacctccg
    41281 gcagccagaa gtgattttca acaaacttgc gcaagtcaaa atcacgatga cggcgcactt
    41341 tacggtagcg gattaaaata tccagcgggt ccattttggg tgcgcagtcg ggaaaggttt
    41401 tactgtcggg gaaaattttc gccgactgaa catgctcgaa cagttcgaga tagcgatcgg
    41461 caggcgtcag cgcatcagag gcgggcagcc cttcaatcat ctcgggttcc ggttctgcct
    41521 cgatcatctc atccagtttt aattcatacg ggtccagttc atagcagaga tcgacttcga
    41581 tcatcagttc gtctggatta gggttttgaa ttttctgatt gagcataagg atccaacctc
    41641 cgaaattcgt cgctaattat cggcttttct ttaagggtag tcattcgctc cgcctggtgg
    41701 gcgacaaagc gtgcggtgga tcacgtttat taacttgtca aaaaatataa cgcactaata
    41761 tcaatggaat ttataaaaat acggatttgg tgttgttttt cattttcaga acattcaatc
    41821 ctgctctcgc cgcaatcatg atatgccatt cctgatctgg gtaataggca caggctatct
    41881 tattgatagt ttatattcat gtaattgatt gattcctatc aaaaagcgta tctccctgtt
    41941 ttttatgatg gctttgtctc gcagagacgt ttgcagcatt ctggaaaatt ttctgggtca
    42001 caataactca ccaccgtgag ggaagaataa tgaaaatggt ctcacgtatt accgcgatcg
    42061 gcctggctgg cgtcgcgatt tgctatttag ggttatctgg ttatgtgtgg taccacgata
    42121 ataaacgcag taaacaggcc gatgttcagg catctgctgt cagtgaaaat aataaggttt
    42181 taggctttct ccgcgaaaaa ggatgcgact attgccacac gccttcggca gaattacccg
    42241 cctattatta tattcctggc gcgaaacagt tgatggatta cgacattaag cttggatata
    42301 aatcttttaa cctcgaggcc gtgcgtgcgg cactgctggc tgataaaccc gtttcgcaaa
    42361 gcgatctgaa taagattgaa tgggtgatgc agtatgaaac tatgccacca acgcgttata
    42421 ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg ggcggaaata ctggcctgga
    42481 ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac tgctccggag catcgcaatg
    42541 aaccggtgca gcccatcccg caaaaactgc ctaccgatgc gcaaaaagtg gcgttgggct
    42601 ttgcgctgta tcacgatccc cgtttatcgg ctgatagcac catttcatgc gctcattgcc
    42661 atgcgttgaa tgcggggggc gtcgatggca gaaaaacatc gattggtgtt ggtggcgcag
    42721 ttgggccgat taacgcgccg acggtattta actcagtatt taacgttgag cagttctggg
    42781 atggtcgtgc ggcaacattg caggatcagg caggtggacc gccgttgaac ccgattgaaa
    42841 tggcgtcgaa atcctgggac gaaattattg ctaagctgga aaaagatccg cagcttaaag
    42901 cgcagttcct cgaagtttat ccgcaaggtt tcagtggcga aaatattact gatgccattg
    42961 ctgaatttga gaaaacatta attacgccgg attccccatt tgataaatgg ttgcgtggag
    43021 atgaaaatgc gctgacggcg caacagaaaa aaggctatca attatttaaa gataataaat
    43081 gtgcaacttg tcatggtggt attattctcg gcggacgttc ctttgaaccg ttggggctga
    43141 aaaaagactt taactttggg gaaattacgg cggcggatat tggtcgtatg aatgtgacta
    43201 aagaagagcg tgataaattg cgccagaaag tacccggttt acgtaacgtt gctttaacgg
    43261 caccgtactt ccatcgcggt gacgtgccga cgctggacgg ggcggtgaaa ctgatgctgc
    43321 gctatcaggt aggcaaagag ctgccgcagg aggatgtgga tgatatcgta gctttcctgc
    43381 acagtctgaa cggggtgtac acgccgtata tgcaggataa acaataacta atttgatcgc
    43441 ccgaacagga atgtttgggc gatttttatt acgataataa agtctgtttt taatattatc
    43501 atgttaaatg tttatattat aaaaagtcgt ttttctgctt aggattttgt tatttaaatt
    43561 aagcctgtaa tgccttgctt ccattgcgga taaatcctac ttttttattg ccttcaaata
    43621 aatttaagga gttcgaaatg gaccagaagc tgttaacgga tttccgctca gaactactcg
    43681 attcacgttt tggcgcaaag gccatttcta ctatcgcgga gtcaaaacga tttccgctgc
    43741 acgaaatgcg cgatgatgtc gcatttcaga ttatcaatga tgaattatat cttgatggca
    43801 acgctcgtca gaacttggcc actttctgcc agacctggga cgacgaaaac gtccataaat
    43861 tgatggattt gtcgatcaa
//
LOCUS       DAJTYD010000010        42529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTYD010000010 DAJTYD010000000
VERSION     DAJTYD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.010"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..369)
                     /locus_tag="OO585_000529"
     CDS             complement(<1..369)
                     /locus_tag="OO585_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR6628220.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGALGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISF"
     gene            complement(338..1546)
                     /locus_tag="OO585_000530"
     CDS             complement(338..1546)
                     /locus_tag="OO585_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCR6628221.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIQE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(1546..3126)
                     /locus_tag="OO585_000531"
                     /pseudo
     CDS             complement(1546..3126)
                     /locus_tag="OO585_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(3158..3805)
                     /locus_tag="OO585_000532"
     CDS             complement(3158..3805)
                     /locus_tag="OO585_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6628222.1"
                     /translation="MIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNAI
                     SRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNN
                     YENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEKI
                     KQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            complement(3802..3981)
                     /locus_tag="OO585_000533"
     CDS             complement(3802..3981)
                     /locus_tag="OO585_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR6628223.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFFMSEMSKKINSTN"
     gene            complement(4241..5494)
                     /gene="amiC"
                     /locus_tag="OO585_000534"
     CDS             complement(4241..5494)
                     /gene="amiC"
                     /locus_tag="OO585_000534"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6628224.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            5726..7057
                     /gene="argA"
                     /locus_tag="OO585_000535"
     CDS             5726..7057
                     /gene="argA"
                     /locus_tag="OO585_000535"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6628225.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7119..8945)
                     /gene="recD"
                     /locus_tag="OO585_000536"
     CDS             complement(7119..8945)
                     /gene="recD"
                     /locus_tag="OO585_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6628226.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(8945..12487)
                     /gene="recB"
                     /locus_tag="OO585_000537"
     CDS             complement(8945..12487)
                     /gene="recB"
                     /locus_tag="OO585_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6628227.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12480..15368)
                     /gene="ptrA"
                     /locus_tag="OO585_000538"
     CDS             complement(12480..15368)
                     /gene="ptrA"
                     /locus_tag="OO585_000538"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6628228.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(15544..18912)
                     /gene="recC"
                     /locus_tag="OO585_000539"
     CDS             complement(15544..18912)
                     /gene="recC"
                     /locus_tag="OO585_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6628229.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(18925..19248)
                     /locus_tag="OO585_000540"
     CDS             complement(18925..19248)
                     /locus_tag="OO585_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6628230.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19233..19640)
                     /locus_tag="OO585_000541"
     CDS             complement(19233..19640)
                     /locus_tag="OO585_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6628231.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(19637..20200)
                     /locus_tag="OO585_000542"
     CDS             complement(19637..20200)
                     /locus_tag="OO585_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6628232.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(20191..20661)
                     /locus_tag="OO585_000543"
     CDS             complement(20191..20661)
                     /locus_tag="OO585_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6628233.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(20846..21640)
                     /gene="thyA"
                     /locus_tag="OO585_000544"
     CDS             complement(20846..21640)
                     /gene="thyA"
                     /locus_tag="OO585_000544"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6628234.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(21647..22522)
                     /gene="lgt"
                     /locus_tag="OO585_000545"
     CDS             complement(21647..22522)
                     /gene="lgt"
                     /locus_tag="OO585_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6628235.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(22673..24919)
                     /gene="ptsP"
                     /locus_tag="OO585_000546"
     CDS             complement(22673..24919)
                     /gene="ptsP"
                     /locus_tag="OO585_000546"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6628236.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVVAFMERRGM
                     GGLIRGGL"
     gene            complement(24932..25462)
                     /gene="rppH"
                     /locus_tag="OO585_000547"
     CDS             complement(24932..25462)
                     /gene="rppH"
                     /locus_tag="OO585_000547"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6628237.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            26147..26836
                     /gene="mutH"
                     /locus_tag="OO585_000548"
     CDS             26147..26836
                     /gene="mutH"
                     /locus_tag="OO585_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6628238.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            26905..27618
                     /locus_tag="OO585_000549"
     CDS             26905..27618
                     /locus_tag="OO585_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6628239.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            27756..27974
                     /gene="ygdR"
                     /locus_tag="OO585_000550"
     CDS             27756..27974
                     /gene="ygdR"
                     /locus_tag="OO585_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6628240.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            28082..29122
                     /locus_tag="OO585_000551"
     CDS             28082..29122
                     /locus_tag="OO585_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6628241.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLNLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGGMRYLHLADKHDLPSIVTIQTPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(29154..30347)
                     /gene="lplT"
                     /locus_tag="OO585_000552"
     CDS             complement(29154..30347)
                     /gene="lplT"
                     /locus_tag="OO585_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6628242.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(30340..32499)
                     /gene="aas"
                     /locus_tag="OO585_000553"
     CDS             complement(30340..32499)
                     /gene="aas"
                     /locus_tag="OO585_000553"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6628243.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            32888..33919
                     /gene="galR"
                     /locus_tag="OO585_000554"
     CDS             32888..33919
                     /gene="galR"
                     /locus_tag="OO585_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6628244.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(33926..35188)
                     /gene="lysA"
                     /locus_tag="OO585_000555"
     CDS             complement(33926..35188)
                     /gene="lysA"
                     /locus_tag="OO585_000555"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6628245.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            35310..36245
                     /gene="lysR"
                     /locus_tag="OO585_000556"
     CDS             35310..36245
                     /gene="lysR"
                     /locus_tag="OO585_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6628246.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(36232..36924)
                     /gene="ygeA"
                     /locus_tag="OO585_000557"
     CDS             complement(36232..36924)
                     /gene="ygeA"
                     /locus_tag="OO585_000557"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6628247.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(37053..38468)
                     /gene="araE"
                     /locus_tag="OO585_000558"
                     /pseudo
     CDS             complement(37053..38468)
                     /gene="araE"
                     /locus_tag="OO585_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(38783..39544)
                     /gene="kduD"
                     /locus_tag="OO585_000559"
     CDS             complement(38783..39544)
                     /gene="kduD"
                     /locus_tag="OO585_000559"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6628248.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(39574..40410)
                     /gene="kduI"
                     /locus_tag="OO585_000560"
     CDS             complement(39574..40410)
                     /gene="kduI"
                     /locus_tag="OO585_000560"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6628249.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(40697..41878)
                     /locus_tag="OO585_000561"
     CDS             complement(40697..41878)
                     /locus_tag="OO585_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6628250.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            42133..>42529
                     /locus_tag="OO585_000562"
     CDS             42133..>42529
                     /locus_tag="OO585_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6628251.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSL"
BASE COUNT        10817 a        11380 c        10089 g        10243 t
ORIGIN      
        1 aaaagagatc aaataattac ccattttttt caaactaggc agtgtcgcca ggatttccgc
       61 cgtctcacca ctgtttgaaa taagaataac aacatcgtct ttcgccacca tcccaagggc
      121 gccgtgtacc gcttccgtac cgtgaacaaa aaacgaggga gttccggtgc tggcaaatgt
      181 tgcagcaagt tttctggcaa taatgccaga tttcccaaca ccaataaaaa caacttttcc
      241 ctggcaagat tgtaatattt taattaaatt cgcgacttcc gaaagggggg aatcaataac
      301 ctcttctact gctttatgtt cgttacgcat aaaacgttta ataagatgga taagatccgt
      361 attgttcatt ttctttttcc tgattaatat ttttaattac actcgcaagg cgcgcaagcc
      421 ccatatataa tttttctcga ctgcaggcca gattaagacg cagatagccc tctccttcag
      481 taccataaac atgccccgcc ataataccta ctctcccttc gttaattaat cgacggtaaa
      541 atttatcaaa gggataacca agggccgagc aatcaaccca cgaaaaatag cagccctcag
      601 ctggccatac gatcaattcc ggaatatttt catttaaata ctgcaaggta aattgaatgt
      661 tagaggcaat atgtaccttt aatgacttta accattcttc acattcatta taggcaacaa
      721 tggtcgcaat tacattcagt actgatgggg agtttacgaa atcgcgatac ctcgatatcg
      781 ttctgaactt atgattaaaa ttatcgtcag ttgaaatcat atatgatccg gttagcgcag
      841 gaacattaaa tgttttcgaa atcgaattaa acaccaccac tttgtcatct tttattgtta
      901 gcgctgacgt gaaactatga ttaaaaacaa aatcactatg tatttcatct gaacataacc
      961 aaatattatg ccttgcacag catgctgcga ttctgtgaag atcgtcttcc gaccagaccg
     1021 ttcctgtcgg gttatgagga ttacataata acaccatctt cgcgctttta attttaagct
     1081 caaagtcttg ccagttgata acgtagcctc tcccattttc tttcatcaaa gaggttgtta
     1141 gcatgtttcg accattatgc ttaatcacat caaaaaatgc gttataaaca ggcgtgaaaa
     1201 tgagaatatt atctccattc aatgtcaata tttctattgc cttagatatt gaatacatca
     1261 cgcttgggct ataggtgatc cattctggtt gaatctctgt tttatattgt tgatgatacc
     1321 aattgactat cgaattcaga tagtcatcat gctgccaacg actgtaccct aaaatcgggt
     1381 gttccagacg tttttttaat gttctgatga tagcttcctg tatcggaaga tccatatctg
     1441 aaatagagaa tggtaataca tcttttacac caaatctgtc ttcacaaaag tcccattgtg
     1501 tgcaatatgt tcctttacga gaaattgagg tattaaaaat atccatcaac atatccccat
     1561 gtaattatcc atatcccgac gtagggcgat gacttttgta ccgataatga cttgtaaggt
     1621 attttgatca agtttcacaa ccgcaattcc tccggccctt tttattgcag cctcatttat
     1681 caatcccata tccgcgatag ttaaacgcaa acgagttaca caattatcca gtgatttaat
     1741 attttcttta ccacccagag ccttaaccat ttgctccgct gcctgcaact ctgatttatc
     1801 atgaactggg atgttagcta ttgcagtttc ttcgtgagcg ttcagcgtga caggactgga
     1861 ctcttcacgg cctacagtca tcaggttaaa tttgagaatg gtccaacgga acacaaagta
     1921 ataaatgaag aaataagcca cttcaacaca taccgctaat ggccatcccg ttcttgtgcc
     1981 acgtaagaca ccaaagctaa taaattgaat cagatcgcca gtaaagccca tcttcacgcc
     2041 catatatggc agaaccatgt tagccaatcc attcataaag gcatggaaga caaacaacag
     2101 cgggctgaca aataagaaca gaaattcaat cggctcgctg acgccgccaa taattaccgt
     2161 gagcacaccg gaaatcagca atgctttgat agcccttctg ttttgagcat gagcagtgcg
     2221 atacatcgcc agcgcggctc cagccagacc atactgaatc atcagtttgc cctgctccat
     2281 tttccccgcc aaatccagcg gaatgaccgc attctctttg acgtatgcca taaacatgtt
     2341 cagggtgccg taatactctt caccgccaat cactgccgat ccgccgattg gggtaaagcg
     2401 gaaaactgat gtcaccaagt gatttaagcc aaaaggtatc gtcacgcgtt cggcaacagc
     2461 atagaagaaa taaccaacag gaccggaagt tgaaatccag tgaccaatga gcatgaaaag
     2521 attgaaaaat ggcggccaga taaaaggaat gatcagacca aatccactca tcacaatcag
     2581 tgtaatgata ggcacaaggc gtgggccact ataaaaccct aattattcag gaatacgtaa
     2641 gttaacgatc tttttataca tgctggcgac taataaccct gccacaatcc cacccaacac
     2701 gctggtattg taggactgga tcccgagtat gatggtttgc ccatgtgtcg acatttggtc
     2761 agcagctacc aataagtcgt gctgtttaag ataaaagttc gttccaagat gcatcgccat
     2821 aaagccaatt aagccagaaa aagcgccata ggctttatca tctttctctt ttaataatcc
     2881 caaaggtata gctatcgcaa acaatacagg taaattaacg aaggcaaaca aaccaaggct
     2941 aacaataaaa tcaagtatgg ttttaattaa cggaatagcc aacactggaa tgaattttgc
     3001 catatcatcg ctggctaaac cacttcccag ccctaacatc ataccacata cacttaacag
     3061 agcgatggga tacataaatg ccttccccag gctctgaaaa aaactccagg atttcttttg
     3121 tttcatactg gttatctcat gaaaatgtta tgtataatta gtccattaat actttgatgc
     3181 gaatagaaag atatagtttc tcttccagaa tcaaatcata tttaaaagtt gcgtacagat
     3241 atctttcaat ttcatgaatg gcacgttgta actgaaaatc tttttcaaga aactgctcat
     3301 agatatcacc aatatttttc tgttttattt tttcattaca taacacacga ctagaaaaat
     3361 atttaaggtg catcataaat ctggagtagt taaatgaact tctgtccacc cttctgttat
     3421 aaacaacttc aataagctcc gtgatttttt gagacagctc aataatcctt actgattcat
     3481 aatcaactga attttcataa ttatttaaaa tatgcaatgc tataaaacca atctcatcat
     3541 ccggtagttg aacgctaaat tgctcattaa tgattgttaa tgcatcacga cttattgcat
     3601 actctgcttt gtacaacgct ttgatttcct caaggaaaac attattaatt tgtatgcctg
     3661 agacgactcg ggatattgca ttcgaaatgt gatcagcaag aatcagtagt agcgatggat
     3721 tgagtttttt ttcgagatta ctctccgcat aatgcactat cttttcggca acaaaaatat
     3781 actcaatacc cacatgctca atcaatttgt agagtttatt tttttgctca tttctgacat
     3841 aaaaaatccg gtttgcagga tgctcaggga ccgacatacc atatttttat tgtaaccaac
     3901 gccaggccca gaaatgataa cctcctgtcc gttcaccgat gcctgtacac aattattgtt
     3961 catgactttt tcgatgatca ttccatggct ccccaaaaaa aaggcaaggc atgtcagaag
     4021 taataattac ttcaaacatt gccttgcctg atttaacagt tgcaagataa ttaactaatg
     4081 cgtatacctt acacggaatt gtgcttaata aaagagatga cagacttatt ttttaagccg
     4141 atattcaaat attcgacagc agtcacaaaa ccatcgttca acatttatgt aaataatatt
     4201 tattcaatga aacataaatt cattgaataa attaaaaaca tcatcccctt ctcgccagcg
     4261 tcgccccatc ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct
     4321 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actaataaac gccgtttcga
     4381 ccagaatgga gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat
     4441 ttttatgcag cttgttgatt ttaccgagct tattcagtac cgctttacca aacttcaggc
     4501 tgtcggcaat tgtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
     4561 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg
     4621 ccgcagtact ggttgcacct ttggttgaga gcgcaaacac agaggaaccg ctcggctgac
     4681 gactggtaaa ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt
     4741 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
     4801 tattgccctc tttctcgatc agagagcgca gacggcgagc tatttgcaat accacgtctt
     4861 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccgccgtgg ccagggtcaa
     4921 gcataataac aataggacga tcgcgccctg ctttacccgg ttgtggacca ctttgtgctg
     4981 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
     5041 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
     5101 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatcc
     5161 gtacggtctg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
     5221 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
     5281 ccacgcgttc agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
     5341 ccgtcacacg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctgaccg
     5401 cagccagact gacctgactt acgctcaata gccacatggc acccgcgcct tgcagtaaac
     5461 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
     5521 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
     5581 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
     5641 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata cactaattta
     5701 gaataatcat gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg
     5761 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
     5821 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
     5881 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
     5941 tctggccgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
     6001 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggacatca ctgctcgcct
     6061 gtcgatgagt ctcaataaca cgccgctaca gggcgcgcat atcaacgtcg tcagtggcaa
     6121 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
     6181 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gacagcggtg caatagtgct
     6241 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
     6301 tgccactcaa ctggccatca aactgaaagc tgagaagatg attggttttt gctcttcaca
     6361 gggcgtcact aatgacgacg gtgatattgt ctcagaactt ttccctaacg aagcgcaagc
     6421 gcgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
     6481 tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga
     6541 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
     6601 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
     6661 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
     6721 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
     6781 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
     6841 ccgcagttca tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag
     6901 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
     6961 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
     7021 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaattg tcggatgcga
     7081 catgcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
     7141 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
     7201 tcggcataca gcgacagacg gcgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
     7261 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
     7321 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
     7381 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
     7441 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc
     7501 accggtcgac cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
     7561 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
     7621 cacaaaagtt ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
     7681 agcagatcca gataacgtcc ataacccgca agagcttcct caagcatcgc aatataatct
     7741 tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
     7801 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg
     7861 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
     7921 ccggttcctg ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg
     7981 gtaaagcccg cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
     8041 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc
     8101 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
     8161 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat
     8221 cggtgcaaag tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
     8281 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
     8341 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc
     8401 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
     8461 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
     8521 tccagggttt gcgccagtag agcttcatca acctcaatgg catgattcac ctcattgaag
     8581 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
     8641 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
     8701 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
     8761 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
     8821 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
     8881 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
     8941 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
     9001 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
     9061 ggaacagata gataacgccg ccaaagtgac gatcatagtc gtaatcagca atgcgatggc
     9121 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
     9181 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
     9241 atttatagtc gagcaggtaa taacgccctt cgtggcggaa tactaggtcg ataaagcctt
     9301 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcggatcaa
     9361 actggcgaat tagtgcatca agctggctag cgatgagcgg ttcgctaatc ggcaggtaaa
     9421 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaacc cccttttcat
     9481 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
     9541 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
     9601 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
     9661 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
     9721 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
     9781 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
     9841 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
     9901 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
     9961 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    10021 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    10081 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttttggcg
    10141 cagcattaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    10201 ggacgcggaa attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt
    10261 tatggatcgt gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg
    10321 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    10381 tttccagctg cgttccggct tcttgtagca gttcgctgat atgcaagata tcggtaagac
    10441 gccgctcacc gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg
    10501 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    10561 caactaccgc atcccacgca tgttcgtcat tattcagcgt ttcgatatcc agcgcgttca
    10621 gccccatcat tgacgttgcc agcgcactac gcagggtgtt ctcacgttcg ggcgtcatca
    10681 ccgcctgcaa taaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    10741 tcgaaaggta aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct
    10801 cctggcggct gcgcaccagc acactgatgt ccgaagcacg caccggacgc gcgtcgtcgc
    10861 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    10921 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    10981 gccacatttt catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt
    11041 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
    11101 ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta
    11161 aagtgtagtg ggcgtgaact tcactacgcg ccttcatata agtgaagata tccgcacccc
    11221 ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    11281 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga
    11341 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcaccg ctttcgctac
    11401 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    11461 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggtgatcacc agatcgcgga
    11521 tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt
    11581 cccccccagc tttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg
    11641 gcaactgata actgttggtc tcttcttctg cccaggcgct gatcttgtcg atccatttag
    11701 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    11761 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtagc aatacgcgcc acaatttgcg
    11821 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcgcttcgc
    11881 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    11941 cgacctgggc tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    12001 cctggtaacg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt
    12061 caaaggcatt caggttgagc atgcgctggc aaaaaccgtg aatagtaaag actgccgctt
    12121 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    12181 cttccagcag acgttcgtac agtggattgt cggtagtttc acgcagacag gcgatgcgca
    12241 actcatggat attgctacgg atacgaccac gcaattctgc cgtggcagcc tcggtaaagg
    12301 tgaccaccag cagttcttca acggtcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    12361 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    12421 cttcaatcag gcgctcaccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    12481 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    12541 actttccagc cttcagggtg tacatattcg gctttcccgt tctggctgcc ggaaatctgc
    12601 gacagaatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    12661 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    12721 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    12781 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    12841 aattttgcct ctgtggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    12901 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    12961 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    13021 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    13081 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    13141 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    13201 tgtttttgca catcgcgtgc ctgcgttgtt gcctgggcct cggtcatgtt gccgataacc
    13261 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    13321 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    13381 atctgcgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    13441 tggttatacc aagacttcgc ctgctcaagc tgatcttccg tagcggtata gctaaagtac
    13501 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    13561 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    13621 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    13681 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggttcgctg
    13741 gaaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    13801 ggatggtcat atttcttctc tgacttaatc agcgagaaat cgtcaggaat ataggggtta
    13861 agctccggta aggagagcgc aatattggca gctttttgct gccagtcagc gaaagtttgt
    13921 tcgctgattt tatcgacctg atacggcgca tcgacaaaat aagccgtttt attgtgcggc
    13981 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttct
    14041 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaacc
    14101 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    14161 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    14221 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    14281 gccaggcctt tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    14341 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcggaa
    14401 agtgtacctg ggctgcgatt accaatcaga taggtaatca attcatccgt tttactacgg
    14461 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca gaactttacg cggcagtgcc
    14521 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    14581 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    14641 tccggcagcg gtttattgct gtaaataacc gccttcatca gattggcgga atagtacttc
    14701 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttatcgct taaagtttcg
    14761 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    14821 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt caccgcatta
    14881 cgctcacgtt cggcatattt cttgtcgagc aaaggttcag caatagcatc ggccaggcgg
    14941 tctaccgcac caggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    15001 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    15061 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    15121 tacgcctcgg gatcttccag cgacccaacg ggcaccacca gcgccgagag cgatttaact
    15181 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    15241 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    15301 gcctgactta agggtgccca aagggcaact aacaacaata atgctttgaa ccaagtgctg
    15361 cggggcattc acggacctca taatcaactt aattttctgt ccagattcaa cacgttaacg
    15421 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca
    15481 tcagtccgtg actggtgcgc atcataaagt aagcggatag attgcgcaat ttttatacag
    15541 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    15601 ggcctccatt gtctctggtg ttaattgccg ccagagcctt tgataccaga tattatcacc
    15661 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    15721 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    15781 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac atcccttcac gatacccctc
    15841 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    15901 gccgtctttg cgtagaaaaa ggcgactttc accattacca ccgctggcac agtagacaag
    15961 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    16021 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    16081 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    16141 catctcctgg cactgagttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    16201 tgccgcccgg aagcgacgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    16261 taactgctga ttgatttgat aacggctaag cccttccaga ataaatggct cggtgtcggg
    16321 gatttcgctg tcttcggtac ggaagttcac ctgcaaacgc atctggaaaa atgcccgcac
    16381 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    16441 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    16501 acgagcatag ctttgtcgtt cgcctggctg gtagttttgt ggatcaaacg gcatccgggt
    16561 atggaggcag gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    16621 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    16681 cgggaaacgt tcactgttat cctgaatgga aagcccgata taactgatat agagtttttg
    16741 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    16801 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    16861 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    16921 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    16981 acgctgtgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcccc
    17041 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    17101 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    17161 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    17221 cagatgcccc accagttctg caattaagcc gctcgactca tcataaggta gaaccgattg
    17281 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    17341 ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc
    17401 ccaacgaatg ccggattcgt tgacccacag gcgtaaataa cgcagccctt cttcggtgat
    17461 gtcaaaccgc gccgccagca ccggcacatc cagtaatgcc agcacgtctt ccgacacaaa
    17521 gcggctgtct ggcaacgata acaggctgat aaacgcttcc agtaccggat gcgactgccg
    17581 cgcccgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    17641 agcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    17701 agtaagtgtc gggtcttctt ccagcatcgc cagcaggcga tcgtgcaaaa cttcaacttc
    17761 acgctgcggg ctatggcaaa cgtggaaggt gatactgcta tccagtggat caagcgggcg
    17821 tttgttatcg ctacgggaaa actcttcgat gttcacacca gcaacggcac ggttttccag
    17881 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtgacat cgacaaaggc
    17941 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    18001 cccccatgag gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    18061 tccggcattt tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    18121 ctgacgagtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    18181 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    18241 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    18301 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg
    18361 ccagcgcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggcgcttg
    18421 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccatt gtgccagcca
    18481 ctccgggcga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    18541 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgtg
    18601 ctccaacagt tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    18661 gctctctttg gggatctccg gtaacacccg gacgaacata tcccagataa agctcgcagg
    18721 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    18781 ctgtgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    18841 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    18901 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    18961 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    19021 tgtctgcaat cggttgacct gccagttagc tggtggcgaa atcgcgcgca gttgcgtttg
    19081 ctgccagcca tgccgccaga gctgttggta ctggtttctg ctggcaagac tgttcatcaa
    19141 tgttcgctgg aaacccgata acgcagtgac aatcatcacc atcaacacca tcgccaacat
    19201 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa cgccctctct
    19261 ttcagcggac aaaaatcgct ccagccgctt ggcgaaaaaa caatgtttcc atcaatgact
    19321 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    19381 gccagtaaac gcaaacaaac ctgggcatcg gtttcagcgt actgcgagca ctgaactgct
    19441 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    19501 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgttggctc
    19561 attccttgta atagcaaact acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    19621 acaccctttt cgcggttcac aggttgaatc ctgtcacgct atagctggca ttcaccacgg
    19681 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    19741 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    19801 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    19861 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccgttac
    19921 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    19981 cggtacagat gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    20041 gcagccagat ttcatcttcc agcgccagct tacgggtgct cgttaaactt tcacgctgta
    20101 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    20161 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    20221 acaaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    20281 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    20341 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    20401 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    20461 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    20521 ccgcgcctgg ctggcggttt gccatagccg ttgcgactgc tgccagtatt gccagccata
    20581 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    20641 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    20701 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    20761 tttcgggaag gtgtctcgaa gaatttaatg gagggtaaaa aaaaccgacg cacactagcg
    20821 tcggctctgg caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgcg
    20881 gagcgtagcc ttcaatctca aaatcttcga aacggtagtc gaagatggat tcgggtttac
    20941 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    21001 ccatatgatt gctgtacagg tgcgtgtcgc cccccgtcca gacaaaatcg cccacttcca
    21061 gatcacactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttaaacggca
    21121 ggccgaggaa aacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    21181 catagaactg gaagaacgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    21241 tccacgctga aacaataatg cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg
    21301 tagtgatctg gtcaatatga cgaccatccg gcgttggcca ggcgcgccac tgtttaccat
    21361 acactggccc gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    21421 cgtgtagata agcaatgtta gtgtcgccct gcagaaacca cagcagttca tggatgatgg
    21481 aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    21541 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    21601 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    21661 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    21721 tggaaagaat ttgtcccatg ctgatgtact gcacccaggc accagtaaac tgcgcgtcgg
    21781 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta accaatcagg aacaaacctg
    21841 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacta
    21901 caccttccag caacagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtat
    21961 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    22021 cagggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    22081 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag
    22141 agacctggaa gaaggaacgt ttagtacggc gggcgaagat aatcatcacc acgataacgc
    22201 caatcaggcc gccgtggaag gacatgccgc cgtcccagac acggaacaga tacagcggat
    22261 cggccataaa ctgcgggaag ttgtagaaca gaacataacc aatacgtcca ccgaggaaga
    22321 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccactgcccg
    22381 gacgattcgc ccgacgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    22441 ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    22501 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    22561 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    22621 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    22681 ctccgcgaat cagcccgccc atgccgcgac gctccataaa ggctacaacc tgatgacgaa
    22741 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcggcaaaat
    22801 caatgcgccg cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc
    22861 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    22921 aacggagatc gataccatgt atttccgctt cccgggcgat cattgccaga gctcgtaaca
    22981 ttgcaggatg aaggctgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca
    23041 gaatgtattg agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    23101 gcggcagcat aaataccatt gacggtactt ccagcatgat gccgatacgt ggtttgggaa
    23161 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    23221 cgtcaacttc atcgaggctg gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    23281 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    23341 tgcgaatccc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    23401 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca
    23461 gcatcccctg atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga
    23521 atgggatttc agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat
    23581 gttccgggct taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg
    23641 cgggtaaatt gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc
    23701 gctgatattc ttgcagcagt atcggctccg gatcgaccag caattcaccg cgatagccat
    23761 caacgatcag cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag
    23821 ggagccccag cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga
    23881 caacaccgac taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg
    23941 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    24001 acagcaatcg ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    24061 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    24121 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt
    24181 gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc tccggcggca aagcgtttgc
    24241 tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc ggtcaatcgt tcgcgttcca
    24301 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    24361 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc
    24421 gatattgccc aaacaaggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg
    24481 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    24541 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    24601 aacgttcttc ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    24661 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    24721 ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc
    24781 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    24841 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    24901 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    24961 tgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    25021 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac
    25081 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    25141 cgccgctcac cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    25201 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    25261 ggattcgaac gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct
    25321 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    25381 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    25441 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    25501 gtttcatact ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    25561 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    25621 tattcacctt ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg
    25681 gagcattctg aataagcccg atataggcat tttaaaatat tcattataag tcaatgcatt
    25741 acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac
    25801 gggttggcga aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc
    25861 actgaaccac taaaaactgg agtttcgtcg cacgtcaagg ctgtaaatgg aaacagtagt
    25921 ggaggttttt cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat
    25981 tttcagtgac cagatttgga aaacctattg cagtgctgcg caactggatt atcggcagcc
    26041 taaaaagcaa tataaatcag agaattgaac aacacatgtg gaaaaagtta cactgcgaat
    26101 attcggtaca taattgctgt ttgtttttta accaaggtat catgacatgt cccaacctcg
    26161 cccactgctc tctcctcccg aaactgaaga acagttgtta gcgcaagcac agcaactttc
    26221 tggttataca ttgggagaac tggcggcact tgccgggctg gttacgccag agaatttaaa
    26281 acgcgataag ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag
    26341 taaacctgag caagattttg ctgctctggg cgtggaactt aaaactatcc ctgtggatag
    26401 tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg gtaatagcgg
    26461 ggtgacctgg gaaaccagcc acgtgcgcca caagctcaaa cgcgtactgt ggataccggt
    26521 tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta ggatccccgt tgctgtggac
    26581 cccgaatgaa gaggaagacc gacagctacg cgaagactgg gaagaattaa tggatatgat
    26641 tgttctcggt caggttgagc ggatcaccgc tcgtcacggg gaatatttac agataagacc
    26701 gaaagcagcg aatgcgaaag cgcttaccga agccattggt gcccggggcg aacggattct
    26761 gacgctgcca cgcggctttt atttgaagaa gaatttcacc agtgcactac tggcccgtca
    26821 ttttctgatc cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt
    26881 catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg
    26941 cttgcgctcg gtacgctgac gctgctggag atcgttcttg ggatcgacaa tattattttc
    27001 ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg ctcatgcgcg ccgtctgggg
    27061 ttggcgggag ccatggttat gcgtctggcg ctgctggcat caatcgcctg ggttacgcgc
    27121 ctgacaaatc cgctttttac aatattcagt caggaaattt ctgcccgtga tttgattctg
    27181 cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga atccattgaa
    27241 ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt
    27301 atgctgctgg atattatctt tagcctcgac tcggtgatta cggctgtggg tctgtcagat
    27361 cacctgttta ttatgatggc agccgtggtg attgccgtag gcgtgatgat gttcgccgcg
    27421 cgctcgattg gtgattttgt cgaacgccat ccttcggtaa aaatgctggc gctctctttc
    27481 ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt
    27541 tacatctact tcgcgatgtt cttctctatt gcggttgaaa gcctcaactt gattcgcaac
    27601 aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcactccttt
    27661 taccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcgttt tataaacaga
    27721 ctattatcat aggtaggcca aatgaggata accggatgaa aaaatgggca gtaataattt
    27781 ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca
    27841 aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg
    27901 tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa
    27961 ttattgaacg ttaacaaata aggtcagcat ccggctggcc ttaagatttt tctctttccc
    28021 ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa aggaaacggc
    28081 tatgcaatat caccgtatac cccacagttc gctggaagtc agcacgctgg ggcttggcac
    28141 gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg actatgccgt
    28201 cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga
    28261 aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga
    28321 aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca agggcatccg
    28381 cccggatcag gcgctggatc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg
    28441 cctacagact gattacctca atctttatca ggtgcactgg ccgcagcgcc cgaccaactg
    28501 cttcggcaaa ctcggttata gctggacgga ttctgcgcct gcggtttcgc tgctggatac
    28561 gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa
    28621 cgaaactgca tttggcggaa tgcgctacct gcatctggcg gacaaacacg atctgccgag
    28681 tattgtcacc attcagaccc cttacagtct gttaaaccgc agttttgaag taggtctggc
    28741 agaagtcagc cagtatgaag gggtcgaact gctggcctat tcatgcctgg gtttcggcac
    28801 gctgaccggg aaatatctca atggtgcaaa aaccgctggc gcacgtaata cgctctttag
    28861 tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat
    28921 cgccagacgt catggcctgg accctgctca gatggcgctc gcgtttgtac gccgtcaacc
    28981 gtttgttgcc agcactctgc tgggcgcaac cacgatggat cagctgaaaa ctaacatcga
    29041 aagtttgcat ctggagttaa gcgaagacgt attagctgaa attgaagcgg tgcatcaggt
    29101 ttatacttat ccggcaccat aagattaacg ccggttaaaa ccggcgttaa atattaatga
    29161 cggcgctgcc agatccacag cgccgttatt gccagcgcaa acagcgcacc gaagccaatg
    29221 ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc cagcatcaac
    29281 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    29341 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccatcacg
    29401 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga aaaaatcagt
    29461 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaact
    29521 aacttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    29581 gtgggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    29641 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    29701 ccattgcgcc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    29761 caagactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca
    29821 ccaccgtagg ccagtgcgca tgcggccagg gcgacgagga catgccagtc agccagcaca
    29881 ccaccggcta cggaaccgag caaaatcgcc gctatggtag aagcttccat taaaccgtta
    29941 gctttcacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    30001 taggctgcag caccaacccc caccagcgta tagccgagaa acggattgat accaaagcag
    30061 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    30121 aagctatccg ccacctgccc gacaaacggc gcaaaaagaa tgtaagcacc tacaaacacc
    30181 atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa cgccagagtg
    30241 gcaaacagta gggcattatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    30301 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    30361 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    30421 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    30481 atactgttgc aacttatcga gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    30541 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    30601 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    30661 tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa cgcacaatat cgccagtgtc
    30721 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt ccagtacacc
    30781 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    30841 gccctcttcg ataccaggaa ccgacaacag gcgcgcatcc attcctggta gaatacgccc
    30901 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcactcggt
    30961 tacaccatag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    31021 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    31081 ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg aacaacacgg tgcaactgcg
    31141 gtcatacacc aactccggca caatgcggta atgcagcggg cttggataaa ggaacacttc
    31201 tgcacctgta agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    31261 taatgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    31321 gttcgccaga atgcttttat ggctatggac gacgcctttt ggatggcctt cagaaccgga
    31381 ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt acctgcgcca ggcgcggcat
    31441 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    31501 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    31561 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag cactggtcag
    31621 cccttttacc ccagcagtgt agttcatcat tgcgggaatg cggcgacgcg cgatggcccc
    31681 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    31741 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    31801 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    31861 tgcactcagt aaagattcgt acagagtttc acgcgggcgc accgccatac gcgcttccat
    31921 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcgacgcatc
    31981 cggcatcgcc acctgcgttg gaggcaaaat atgcagagta atttgcggga acaagcgacg
    32041 tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc gccccttcaa tacgcacagg
    32101 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    32161 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    32221 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    32281 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    32341 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    32401 cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    32461 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    32521 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    32581 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    32641 gtaccgaata atctcaccaa tcaatacctc tgggatcacc actttagcaa cctgaagcca
    32701 aacgccacca gcggtcaatc gcaacagcgt ttcgcaaagt gtaaccaaag gtttgcaatc
    32761 tctttttttg tcttacttat ttgcgtttgg ctcacattcc cacgatgaaa acacgccact
    32821 ccttgaacca acgggcgttt tccgtaacac tgaaagaatg taagcgttta cccactaagg
    32881 tattttcatg gcgaccataa aggatgtagc ccgactggca ggcgtttcag tcgccaccgt
    32941 ttcccgcgtc attaataatt cacccaaagc cagcgaagct tcccggctgg ctgtgcatag
    33001 tgcaatggag tctcttagct atcacccgaa cgccaacgcc cgtgcgctgg cgcagcagac
    33061 cactgaaacg gtcggtctga tcgttggtga tgtttccgat ccgtttttcg gtgcaatggt
    33121 gaaagcggtc gaacaggtgg cttatcacac cggtaatttc ttattgattg gcaacggtta
    33181 ccacaacgaa caaaaagagc gtcaggctat tgagcaactg atccgccatc gctgtgctgc
    33241 gttggtcgtc catgccaaaa tgatcccgga tgccgattta gcctcattaa tgaaacaaat
    33301 gcccggtatg gtgctgatca accgtatcct gcctggcttt gaaaaccgtt gtattgctct
    33361 tgacgatcgt tacggtgcct ggctggcaac gcgtcattta attcagcaag gtcatacccg
    33421 cattggttat ctgtgctcta accactctat ttctgacgcc gaagatcgtc tgcaagggta
    33481 ttacgatgcc cttgctgaaa gtggtattgc ggccaatgac cggctggtga catttggcga
    33541 accagacgaa agcggcggcg aacaggcaat gaccgagctt ttgggacgag gaagaaattt
    33601 cactgcggta gcctgttata acgattcaat ggcggcgggt gcgatgggcg ttctcaatga
    33661 taatggtatt gatgtaccgg gtgagatttc gttaattggc tttgatgatg tgctggtgtc
    33721 acgctatgtg cgtccgcgcc tgaccaccgt gcgttaccca atcgtgacga tggcgaccca
    33781 ggctgccgaa ctggctttgg cgctggcgga taatcgccct ctcccggaaa tcactaatgt
    33841 ctttagtccg acgctggtac gtcgtcattc agtgtcaact ccgtcgctgg aggcaagtca
    33901 tcatgcaacc agcgactaac cgcagttaaa gcaattccag cgccagtaat tcttcgatgg
    33961 tctggcgacg gcgaatcaac cgcgcctgac cattatcaaa cagaacttct ggtaacagcg
    34021 aacggctatt gtagttggat gacattgatg cgccatatgc ccctgtatca tgcagtacca
    34081 gataatcacc tgccttcact tccggcaagg cgcgggtttc aacatttccc ccttcctgct
    34141 gggtaaagac atcgcccgat tcacataacg gtccggcgac gacggtttcc accgttggcg
    34201 cgtgttccag agaacgacca tcagctgcca gggcactgat atggtggtaa ctaccgtaca
    34261 ttgccgggcg catcagatcg ttgaacccgg catcaaccag cacaaagtgg cggctcccca
    34321 tttgtttgac gctccgcacc tgagtaatta atacgccaga ctgcgctacc aggaagcgac
    34381 ccggttcaat ttccagtttc acagggtggc ccaaatggcg ggcgatttgc tcacgcgcgg
    34441 cattccacag accataataa tgttcggtat caaccgcctc ttcaccctgt tgataaggaa
    34501 cagaaagccc accgcccgca gaaatagcct gtaaatcctg accgaattcg atgacctgac
    34561 gcaccatagc accacacacc tgttccagat gggcataatc aacgccagaa ccaatgtgca
    34621 tgtgaatgcc gaccagctgc agatgatgac gttgtatcac gtccagtgcg gcgggcagat
    34681 cggtgtacca gataccgtgc ttgctgtttt cgccaccggt attggttttt tggctatgtc
    34741 cgtgaccaaa ccccggatta acgcgcagcc atacccgatg ccctggcgaa acctggccca
    34801 gttggtcgag catatcaaca gaacccgcat tcaccggaat ttgcaattca ctgacgcgtt
    34861 caagcgtcgc ctgatcgata acatctgccg taaaaacaat atcatcgggg tgcgtttgcg
    34921 gattgtaacc cgccgccaac gcacgctcta tttcgcctaa cgagacggaa tccactttca
    34981 cgccctgctc acgcattaag cgcaaaatat gaatattgga acaggctttc tgtgcaaagc
    35041 gcaccacatc aaactgtttc agcgctgcaa tctgccgacg aataatttgc gcatcgtaga
    35101 cccacaccgg gcagccaaat tcagcgggca aacgcagcag attttcggcg gtgagatcgg
    35161 tatcggtgct gaacagtgaa tgtggcataa caaactccag ataagtgctt ttttatgatt
    35221 acgccacatc ataaaaagaa taaaaaatat cgatttatgt cgagtctatg caaaaatgat
    35281 atggattacc ggattgcgag agagcgctaa tggccgccgt taacttacgt catattgaaa
    35341 tttttcatgc ggtaatgacc gccggaagcc tgactgaggc ggcacacctg ctacacacct
    35401 cacagccaac cgtcagccgc gaacttgcgc gctttgagaa ggtgatcggg ctgaaattgt
    35461 ttgagcgcgt acgtgggcga ttacatccta ccgtgcaagg actgcgtctg tttgaagaag
    35521 tgcaacgatc ctggtacgga ctggatcgaa ttgtcagcgc tgcagaaagt ctgcgcgagt
    35581 ttcgccaggg agaactgtct attgcctgcc tgccggtctt ttcgcaatct tttttaccgc
    35641 agctcctgca accctttctg gcacgttatc ccgatgtcag cttaaatatc gtgccccagg
    35701 aatcaccgct acttgaagag tggctctcgg cccagcgtca tgatttagga ctcactgaga
    35761 cactccatac gcctgcggga acagaacgta ccgaattact ctctttagat gaagtgtgtg
    35821 tgttacctcc gggtcatccg ctggcggtaa aaaaggtatt aacgccggat gattttcagg
    35881 gtgagaacta catcagcctt tcccgtactg acagctatcg ccagttgctg gatcagctat
    35941 ttactgaaca tcaggttaaa cgacgcatga tcgtagaaac ccacagcgcc gcgtcaatct
    36001 gcgcaatggt acgggcgggg gtaggtgttt cggtggttaa cccgctcacc gcactggatt
    36061 atgcggcaag cggtttagtg gtgcggcggt tcagtattgc ggttccgttc acagtcagcc
    36121 tgatccgccc cctgcaccgc ccgtcatcag cgctggtgca ggcgtttagt gagcatttac
    36181 aagcggggtt accgaaactg gtcacttctc ttgacgctat tttgtcgtca gctacgacag
    36241 cataaaagcg acagcatcct cggcatggat cgccgcagta tcaaacacag gcaaaacact
    36301 gcgctcttct ggcaccagta aaccaatttc tgtgcagcca aaaatgacgc cctgtgcgcc
    36361 ctgttctgca aggcgagcaa tcacttgcgc ataataagcg cgtgacgctt cggtaaattg
    36421 ccccagacac agttcttcaa aaataatctg attaattttc gcccgttcat ccgcttcagg
    36481 aataagacaa ttgatggaaa attgttccgt cagccgcccg cgataaaaat cctgttccat
    36541 ggtgtaacgc gtacccagca gcgccacacg agtcattccg gccccggtaa ttgcacgtcc
    36601 ggtggcatcc gcaatgtgta agaaaggcag agtgcaacgt gactcaatgg catccgccac
    36661 tttatgcatc gtattggtac ataacacaat accttctgcg cccgcccgct gtaagccaag
    36721 cgccgcctca gccagaatgt ccccggtttt atcccattcc ccgcgacgct ggcactcttc
    36781 tatttcatga aaatcgacgc tatgtagcag cacttgcgca gagtgaagcc caccaagccg
    36841 ctgtttaatg ccttcattta tcaaacgata gtaaggaatg gtggattccc agctcattcc
    36901 tcccagcaaa ccaattgttt tcattcctct ctcctgatgt gtgttaccgc aacaaggctg
    36961 aataaggaac gagacaaacg cctcaacggc caagtgccca atctctatta acgaaaaaag
    37021 ggccggatgt acagcacatc cggcccgtga aatcagatgc cgatatttct caacttctcg
    37081 cctgccatca gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cggaatgagc
    37141 cagaaagtga tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa cgtaccggca
    37201 gcgccaatgc tgtcgagcag cgtcaggaag gtcgccgata atcatattcg acacccagtt
    37261 cgtggtggtc gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca
    37321 caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat
    37381 gccaacagag agccaggaca atccactgga agccgtaccg ttatcaaact gcatcaggca
    37441 atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt
    37501 acccctgctt tatctaccgt aaacaccgca ataaaggtgg cgaacataaa ggtcagccct
    37561 acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa agcccgccat tttgaagata
    37621 cgcggcgcgt agtacatgat gatgttcata ccggtaaact gctgcatcgc ctgcaacaac
    37681 ataccgagaa acacagcacg acggacgtta cggttgatct taaacagtgc ccaaccgccc
    37741 tgttttaact tcaggctttc acgaatttcg ttgagttctt ctcgcgcttt ttccgacgta
    37801 tcgcgcagca tacgcaatac ttcttccgcc tcaatatgac gccccttttc cgccaaccag
    37861 cgcgggctat ttggcaggaa gaccaccaga ataatcagca gaactgctgg taaagcaaga
    37921 acccccaaca ttgcgcgcca gttaccgcta taactgaacg ctgtatcgga taaaaacgcc
    37981 agcacgatgc cgagtgtgac catcaactgg tacatactga tcatcttacc gcgaacgttt
    38041 tcacttgcca tttcagaaag atacagagga gcggtgtaag acgcgatccc gacagcaatg
    38101 cccagcccca cacgagcggc gattaacatc tctacgctgg tcgcaaaagc ggaccctata
    38161 gaaccgagta caaacaggat ggcccccgcc atcaggctgt atttacgccc caggcggaac
    38221 gacagccaac cattaaacag cgcaccaatt gctgcgccga gcatcatgct actaaccacc
    38281 cattcctgca aatgactggt cagcacaaag tgatcggtaa tgaacggcaa cgctccggcg
    38341 attacgccga tatcaagacc aaataacaat cctgcgaccg cagcagctac cgaaacaaac
    38401 atattcatac gccgcgtatc ccgcaaagaa cgtggcgtta aagcagattc cgtattgata
    38461 gtaaccattt tttcctgcca gcagagagta agacatagtg aaaaaatacg tgaacaactc
    38521 acgcaggtgt caggtcggaa acagcataaa tatggattaa attgctgcga catgtcgtta
    38581 tgtgatggat attccaattt tcaaattaag ttgaattatt gagattatta ttaaccacct
    38641 aattttacag cagataaaat tcataaagtt cattaattga taattaatat ggattatttc
    38701 ataaccatga tatggattat gatgatctac aggtataaaa aaccctgcca tgcggcaggg
    38761 tcataaaagt aagaagaatg aattaacgcg ccagccaacc gccatccacg gcaatggtat
    38821 aaccattcac ataatctgaa gcgctggagg caaggaacac tatcggcccc atcaggtcac
    38881 tcggcagtcc ccaacgacca gctggaatgc ggtcgagaat ttccgcgcta cgttgttcat
    38941 ctgcccgtag ttgttgagta ttgttggtcg ccatgtaacc cggagctatc gcattaacat
    39001 taatgttgtg tttagcccat tcgttcgcca tcaatcgcgt cacacccatc acgccgcttt
    39061 ttgatgcggt ataagaaggc acacggatcc cgccctggaa ggagagcatt gacgcgatat
    39121 tgataatctt gccgccattg ccttgcgcga taaagtgttt cgccgctgcc tgagacatga
    39181 agaatacgct cttgatattc aggttcatga catcgtccca gtctttttcg ctgaactcga
    39241 gagcgtcttc gcggcgaatc aatccggcgt tattcaccag gatatcaata tgaccaaatt
    39301 cagctaccgc gcgatccagc agtccaggaa tgccatcaat ctttcgcaga tcggcggtca
    39361 ggcttaaaaa acgacgcccc agcgccgtga cctgctcgat ggtttcagtc ggttcaacga
    39421 tgttaatgcc aacaatgtca cagcccgctt gcgccagccc caacgccatc ccctggccca
    39481 gcccagtatc acaaccagtg acgaccgcaa ctttaccttc gagagaaaat gcacttaaaa
    39541 tcataacaat accttattcg tttatgccca caactagcgc aaatctttaa cggccacatg
    39601 gtccatatca tcaaagacct ggttttcacc aaccatgccc cagataaagg tataagcttt
    39661 ggttccgacg ccggaatgga tcgaccagct cggggagatc accgcctgct cgttatgcat
    39721 cacaatgtga cgcgtttctt gcggttgccc catcatgtgg aaaacgcagg cgtcatcatc
    39781 catattgaaa tagaaataaa cttccatccg gcgctcgtgg gtgtgacacg gcatggtgtt
    39841 ccacaagtta cctggagcca gctccgtcag ccccatactc aattggcagg tttccagcac
    39901 atccgggaca aaatatttgt taatagtgcg acggttactg gtgaggttat cgcctaacgt
    39961 gactggagat acttcgtccg gggtgacttt tttggtggga tacgtcgtat gcgcgggtgc
    40021 gcaattgtaa taaaactttg ccggagtggc ggtatcaata ctggcaaaga caacttcttt
    40081 tgcaccttta ccaacataca gggcgtcgcg gtgaccgatt tcatagcatt ggccatcgac
    40141 agtaatcgta ccggcaccgc caatattgat aacacctaac tcgcgacgtt caaggaaata
    40201 gcttacgccg agttgtttac caacttcccc gccaacggaa accgttttag ttatcggcat
    40261 aatgccgcca acaataattc ggtcaatgtg gctgtaaacc atggtgtact catcggcgac
    40321 aaataccttt tcaaccaaaa attcattgtg cagcccttgg gtatccagcg tttttgcgtg
    40381 cgcactgtgg atgctctgtc ttacgtccac atcaacctcc gataatcagt gaagcgtgaa
    40441 aatagaaaac gatagtgaaa cactgtttcg ttttctctat aaagggatga tatgcccatg
    40501 ggctagggtt ttcaattaaa aataaaacaa tgttttattt ttaagttaag gattaaaaaa
    40561 agtgcagtgt cgatcacgaa tatgtcatga gaatggaaaa atgagggagc ccagaaaatg
    40621 tatgtcgact tgccagccat cattattcaa cagattaatg acggcagata tttatcggta
    40681 aggaggaaat gcagagctac tcgtcacgtt caatggtcaa tgccacgccc tggcccccgc
    40741 cgatacaaag cgttgccagc cctttgcggg cattacgttt caccatttca tgaaccagag
    40801 aaaccaggat tcggcaaccg gaagcgccta tcgggtgacc gagtgcgatc gcgccaccat
    40861 tgacattgac ccgacgctca tcccactcaa gcatcttgcc aaccgaaagc gcctgtgcag
    40921 caaacgcttc attagcctcg ataagatcga catcagccaa ctgccagcct acacgctcca
    40981 ggcaacggcg ggtcgcatac accggcgcaa ttcccatcaa tgccggatct acaccaacgc
    41041 tggcaaatgc gcggatgcgg gccagcacgg gtaaattcaa cgctcgtgct ttggcttcgc
    41101 tcatcatcat taccgcagct gcgccatcgt ttatggatga tgcattcccc gcggtcacag
    41161 aaccgagact atcaaatgaa ggatttaaac gggctaaacc ttctgcactg gcgtcagtgc
    41221 atggctgttc atcggtatca acaaccaacg tctgaccgtt actttggatc attaccggga
    41281 cgatctcatc tttaaatcgt ccggcgtcaa tcgccgctcg cgctttttgt tgcgagctaa
    41341 gtgcgtaagc atcctgcaac tgacggctga tgccatattc gcgagccaga ttttcggcgg
    41401 tgacaccaat atgataatca ttgaaggcat cccacaaccc atcatgcacc agactgtcaa
    41461 ccaactggct attgccaagc tgtgcaccgg tgcggctatc agtcagaaca tgtggtgcgc
    41521 ggctcatgtt ttcctggcca ccggcgatga caatatcagc ctcgccacac tgtatcgcct
    41581 gagtagccag atgcagtgct ttaagcccag aaccgcaaac gtcattaata gtgattgcag
    41641 aaacgctatt aggaagacca cctttaatag ccgattgcct tgccggattc tgccctgccc
    41701 ctgcagtcaa cacctgacca agaattactt catccaccgc atatgcagga acgccggtac
    41761 gttctattaa cgctttcacg accaaactac caagttccac ggcggaatga cccgctaacg
    41821 caccacgaaa gcagccgata ggtgtccgta acgccccgac aatcacaacg tctttcatca
    41881 caacctcgca ccaattaaca gtgcaatagt aaatgattgt taattacagt tatcttaatt
    41941 gtttaaaaaa agtgattttt atcacaaaag aaatatgcct gagcagcagt cagagacata
    42001 actggcacgt aaggtttgca accactaacc caccaataga ggggtagata gggcgttaat
    42061 ctcccatact taacctggtt tatggtaaat tgccctccat tttgtttaat ttgtagatga
    42121 tacgttcaga taatgtctaa tatttggtct aaagaagaaa ctctgtggag tttcgcgctc
    42181 tacggcacag ccgttggtgc aggcacgctc ttccttccta ttcagttagg ttcggcaggg
    42241 gctgtggtcc tgtttattac tgctctggtc gcctggcctt taacatattg gccacataaa
    42301 gctttatgcc agttcatcct ctcatcgaaa acatcagcag gtgaagggat aacgggcgcg
    42361 gtaacacact actatggcaa gaagattggt aatctgatta ccacgctgta cttcatcgcc
    42421 ttttttgtcg tcgtgttgat atatgcagtg gcaattacca actcacttac agaacagctg
    42481 gcaaagcata tggttattga tcttcgcatc cgtatgttgg tgagtctgg
//
LOCUS       DAJTYD010000011        41890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTYD010000011 DAJTYD010000000
VERSION     DAJTYD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.011"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..290)
                     /gene="yeeE"
                     /locus_tag="OO585_000563"
                     /pseudo
     CDS             complement(1..290)
                     /gene="yeeE"
                     /locus_tag="OO585_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(469..1827)
                     /gene="plaP"
                     /locus_tag="OO585_000564"
     CDS             complement(469..1827)
                     /gene="plaP"
                     /locus_tag="OO585_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6628252.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYRVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1817..1879)
                     /gene="yoeI"
                     /locus_tag="OO585_000565"
     CDS             complement(1817..1879)
                     /gene="yoeI"
                     /locus_tag="OO585_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6628253.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2094..3023)
                     /locus_tag="OO585_000566"
     CDS             complement(2094..3023)
                     /locus_tag="OO585_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6628254.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3069..3893)
                     /locus_tag="OO585_000567"
     CDS             complement(3069..3893)
                     /locus_tag="OO585_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6628255.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGLVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3976..4230)
                     /gene="yoeB"
                     /locus_tag="OO585_000568"
                     /pseudo
     CDS             complement(3976..4230)
                     /gene="yoeB"
                     /locus_tag="OO585_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4227..4478)
                     /gene="yefM"
                     /locus_tag="OO585_000569"
     CDS             complement(4227..4478)
                     /gene="yefM"
                     /locus_tag="OO585_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6628256.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4761..4811
                     /gene="hisL"
                     /locus_tag="OO585_000570"
     CDS             4761..4811
                     /gene="hisL"
                     /locus_tag="OO585_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6628257.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4787..4910
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4957..5856
                     /gene="hisG"
                     /locus_tag="OO585_000571"
     CDS             4957..5856
                     /gene="hisG"
                     /locus_tag="OO585_000571"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6628258.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5862..7166
                     /gene="hisD"
                     /locus_tag="OO585_000572"
     CDS             5862..7166
                     /gene="hisD"
                     /locus_tag="OO585_000572"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6628259.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7163..8233
                     /gene="hisC"
                     /locus_tag="OO585_000573"
     CDS             7163..8233
                     /gene="hisC"
                     /locus_tag="OO585_000573"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6628260.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8233..9300
                     /gene="hisB"
                     /locus_tag="OO585_000574"
     CDS             8233..9300
                     /gene="hisB"
                     /locus_tag="OO585_000574"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6628261.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9300..9890
                     /gene="hisH"
                     /locus_tag="OO585_000575"
     CDS             9300..9890
                     /gene="hisH"
                     /locus_tag="OO585_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6628262.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9890..10627
                     /gene="hisA"
                     /locus_tag="OO585_000576"
     CDS             9890..10627
                     /gene="hisA"
                     /locus_tag="OO585_000576"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6628263.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10609..11385
                     /gene="hisF"
                     /locus_tag="OO585_000577"
     CDS             10609..11385
                     /gene="hisF"
                     /locus_tag="OO585_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6628264.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11379..11990
                     /locus_tag="OO585_000578"
     CDS             11379..11990
                     /locus_tag="OO585_000578"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6628265.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12087..13064)
                     /gene="wzzB"
                     /locus_tag="OO585_000579"
     CDS             complement(12087..13064)
                     /gene="wzzB"
                     /locus_tag="OO585_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6628266.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13211..13303)
                     /locus_tag="OO585_000580"
                     /pseudo
     CDS             complement(13211..13303)
                     /locus_tag="OO585_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13473..14477
                     /locus_tag="OO585_000581"
     CDS             13473..14477
                     /locus_tag="OO585_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCR6628267.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14543..15709)
                     /gene="ugd"
                     /locus_tag="OO585_000582"
     CDS             complement(14543..15709)
                     /gene="ugd"
                     /locus_tag="OO585_000582"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6628268.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(15958..17364)
                     /gene="gndA"
                     /locus_tag="OO585_000583"
     CDS             complement(15958..17364)
                     /gene="gndA"
                     /locus_tag="OO585_000583"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6628269.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIT
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFHDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDNAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17469..18302)
                     /locus_tag="OO585_000584"
     CDS             complement(17469..18302)
                     /locus_tag="OO585_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6628270.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
     gene            complement(18299..19168)
                     /locus_tag="OO585_000585"
     CDS             complement(18299..19168)
                     /locus_tag="OO585_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6628271.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            complement(19185..20372)
                     /gene="wzy"
                     /locus_tag="OO585_000586"
     CDS             complement(19185..20372)
                     /gene="wzy"
                     /locus_tag="OO585_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen polymerase"
                     /protein_id="HCR6628272.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            complement(20428..21660)
                     /gene="wzx"
                     /locus_tag="OO585_000587"
     CDS             complement(20428..21660)
                     /gene="wzx"
                     /locus_tag="OO585_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen flippase"
                     /protein_id="HCR6628273.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            complement(21657..22214)
                     /gene="rfbC"
                     /locus_tag="OO585_000588"
     CDS             complement(21657..22214)
                     /gene="rfbC"
                     /locus_tag="OO585_000588"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6628274.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            complement(22219..23097)
                     /gene="rfbA"
                     /locus_tag="OO585_000589"
     CDS             complement(22219..23097)
                     /gene="rfbA"
                     /locus_tag="OO585_000589"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6628275.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(23155..24054)
                     /gene="rfbD"
                     /locus_tag="OO585_000590"
     CDS             complement(23155..24054)
                     /gene="rfbD"
                     /locus_tag="OO585_000590"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6628276.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            complement(24054..25139)
                     /gene="rfbB"
                     /locus_tag="OO585_000591"
     CDS             complement(24054..25139)
                     /gene="rfbB"
                     /locus_tag="OO585_000591"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6628277.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(25512..26405)
                     /gene="galF"
                     /locus_tag="OO585_000592"
     CDS             complement(25512..26405)
                     /gene="galF"
                     /locus_tag="OO585_000592"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6628278.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(26637..27632)
                     /locus_tag="OO585_000593"
     CDS             complement(26637..27632)
                     /locus_tag="OO585_000593"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HCR6628279.1"
                     /translation="MNNNVLLIGASGFVGTRLLETAIADFNIKNLDKRQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(27790..29184)
                     /gene="wcaM"
                     /locus_tag="OO585_000594"
     CDS             complement(27790..29184)
                     /gene="wcaM"
                     /locus_tag="OO585_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6628280.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMSNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(29195..30415)
                     /gene="wcaL"
                     /locus_tag="OO585_000595"
     CDS             complement(29195..30415)
                     /gene="wcaL"
                     /locus_tag="OO585_000595"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6628281.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPTGKIAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLGTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(30412..31692)
                     /gene="wcaK"
                     /locus_tag="OO585_000596"
     CDS             complement(30412..31692)
                     /gene="wcaK"
                     /locus_tag="OO585_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6628282.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMMALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(31968..33446)
                     /gene="wzxC"
                     /locus_tag="OO585_000597"
     CDS             complement(31968..33446)
                     /gene="wzxC"
                     /locus_tag="OO585_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6628283.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCLAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPQGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKPLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQFLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(33448..34788)
                     /gene="wcaJ"
                     /locus_tag="OO585_000598"
     CDS             complement(33448..34788)
                     /gene="wcaJ"
                     /locus_tag="OO585_000598"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6628284.1"
                     /translation="MVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPCITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            complement(34893..36263)
                     /gene="cpsG"
                     /locus_tag="OO585_000599"
                     /gene_synonym="manB"
     CDS             complement(34893..36263)
                     /gene="cpsG"
                     /locus_tag="OO585_000599"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6628285.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTSVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSRDALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(36456..37892)
                     /gene="cpsB"
                     /locus_tag="OO585_000600"
     CDS             complement(36456..37892)
                     /gene="cpsB"
                     /locus_tag="OO585_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6628286.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(37895..39125)
                     /gene="wcaI"
                     /locus_tag="OO585_000601"
                     /pseudo
     CDS             complement(37895..39125)
                     /gene="wcaI"
                     /locus_tag="OO585_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(39122..39601)
                     /gene="gmm"
                     /locus_tag="OO585_000602"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(39122..39601)
                     /gene="gmm"
                     /locus_tag="OO585_000602"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6628287.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLPGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(39604..40569)
                     /gene="fcl"
                     /locus_tag="OO585_000603"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(39604..40569)
                     /gene="fcl"
                     /locus_tag="OO585_000603"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6628288.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQSTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(40572..41693)
                     /gene="gmd"
                     /locus_tag="OO585_000604"
     CDS             complement(40572..41693)
                     /gene="gmd"
                     /locus_tag="OO585_000604"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6628289.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPRFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYCESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIVTGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLDDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(41719..>41890)
                     /locus_tag="OO585_000605"
     CDS             complement(41719..>41890)
                     /locus_tag="OO585_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6628290.1"
                     /translation="IGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPVNVVCRGN
                     PAVVIRERVETE"
BASE COUNT        10962 a        10640 c         9671 g        10617 t
ORIGIN      
        1 ccggtggcac atccacccgc cagaacaatt cccagcccga agatataccc acctataaca
       61 gtaccaggcc acgggaacgc cccggcttcg taagtcagta ggcccgccta aattaacgca
      121 aaaaccccca cactttgtac cgaaattgca atcagcaagg cgtaaaacat gcgattattt
      181 ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg ctgcatgaca
      241 aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat aaattaatta
      301 accagatgaa tgttaatgag gaaattattc atgactggtg gaatgcagac caataaccaa
      361 attctctaaa ttagaacaaa tggttcttaa tgaggatttc gataaaaaag aataaaaaaa
      421 ccggagccaa atattcattc gactccggtt gattattaga aaagatgatt atgctacgtc
      481 ttcgtactgc ggaaccgggt tacggaagct cttggtgacg caagccaggt aaatcagacc
      541 gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc
      601 accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt
      661 cttattacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa cgaaggtgaa
      721 cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga agttgattgc
      781 cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg
      841 tacataaccg aagaagcttt tcgggaatac gccgtcacga cccattacgt acatcagacg
      901 cgctacgcct gcatgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag
      961 cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc
     1021 cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat
     1081 gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc
     1141 atctttggtt tcttccgaca ggttgctgat gccgtcaaag ccggtaaagg agaagcacag
     1201 gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga atggacgagt
     1261 gctcgccagc gtaccagcgc cttcaccttc aaatacgcga taaacaacca tgcccagaat
     1321 caccgcgatc agcaccacct gcaacacgac gattacagtg ttgaagttcg ctacggattt
     1381 cagactacgc aggttaaagg cggtcatgaa ggccaccaac gccaccacaa acatccacga
     1441 tgggatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg
     1501 cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat
     1561 ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa ccagcttccc
     1621 gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg
     1681 accatccgtg aggcctgaaa cgataccaaa agtatcaaac agcgtcatcg gctgcatata
     1741 ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga
     1801 ggtgtttgga gtaacgttat gcgacatggt cattctcctt tacggtgata accgtcgcgt
     1861 aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc gatttttagt
     1921 aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt gaagcaaaaa
     1981 ataaccgacg ctgatgaaac gtcggttttt agtcattttt tgacagcggc gcattgtgcc
     2041 taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt tctttaaatg
     2101 gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt tgctaaaatt
     2161 tcatcccgcc accagccact ggcaagtcct gcggtctgtt cattccagcc aatccagacg
     2221 ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact atcgagaaaa
     2281 tgttgcgcca gataacgggg taaatagccg cagccgaggc cgctaatttg cagctccagc
     2341 ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg tgaggccgta
     2401 gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgctt aataatacga
     2461 cgatttaacg gctcttcttc cagggctaac ggatgatgag gcgcgaccgc aaagacttgt
     2521 tccagatcgc ccaaccgtga aaaaccaaac tcactggaag atggtggctc atgcatcgcg
     2581 ccaacaataa tatccgctcg cccctgagtc agggcatccc atgaaccacc cagtacgcca
     2641 ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat cagtggcgct
     2701 agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc ccagccttca
     2761 tgcagtttga tcgcctgctt ttccagttcc cgcacggtat gcagaacttc ccgccctttc
     2821 tctaataaca tttttccggt gcgggtgaat ttagcgcggt gaccgctacg atcaagcaat
     2881 tgaatattaa ggtcgctttc cagcttgtga acggtgtaac tcagagcgga cggcgtcttg
     2941 tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt ctaaggcatc gaggatcatc
     3001 aacacatcca gcagtggttt catacacgcc cccttgcagt gtatgcggcg tcctgctggc
     3061 gtgatcgttt actccagcgg cattaccagc ggatcgggat actggtattc aaatcccagt
     3121 tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag actatttctg
     3181 aactgcggag gttccagccc cagtaaacgg gccatctgcg gatagaaaac attacgcgca
     3241 gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg taacaacaga
     3301 gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc accatcaggc
     3361 gcggtttttc cggcaaagaa gcgtccggga tgacgccccg gtccgaccag gcccgcaaga
     3421 cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag ttcttctaat
     3481 actagtccac tgttggttac cggattacgc ggggtagttt ctttcaccat gccttgcgca
     3541 tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg ggccagcgcg
     3601 ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggagcc gctacgacgt
     3661 gccggaagcg taatgaccag tgcatcggca tccatcaggg catccagatc gtcagaatcg
     3721 caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat tcgggccgct
     3781 tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc tgaaagtgac
     3841 atcgccagcg gcatgcctaa ccaccctaac ccgacaattg cgaccttttt catccgttat
     3901 ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga caattcattc
     3961 tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg aatcgtcggt
     4021 aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gactagaaac ctgacaaatt
     4081 atgtttcagg ggttctggct tccccttacc ttcaaatggc gttctgcggg tatctttgat
     4141 aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca gataatcatc
     4201 ccatgattcc tcagaccaga ttagtttcac tcaataatgt ccttttctgt tcctttgcct
     4261 gatttcaggc tatcgattga gtccatcaat ctccgggcgt tagcggggga acgcagtaga
     4321 taagccgtct cttccagcga gttgtattct tcgagtgaca tcagaacaca agcctctcca
     4381 ttctgacgag taataaggat cggggcatga tcttcaacgg ctttcatcat tgttgccgac
     4441 aaattctgac gcgcttcgct gtagctaatt gtacgcatgt caatctcctc ttttgtacag
     4501 ttcattgtac aatgatgggc gttaattaac tatttattaa ttagtttgta gatcaaggta
     4561 ttgtcagtga gacgaaaatc caggcttcgc tatttttggt gccgtcagct tagaggacag
     4621 tcctcttagc cccctccttt ccccgctcat tcattaaaca aatccatcgc catagaatat
     4681 ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca tcagttgaat
     4741 aaacattcac agagactttt atgacacgcg ttcaatttaa acaccaccat catcaccatc
     4801 atcctgacta gtctttcagg cgatgtgtgc tggaagacat tcagatcttc cagtggtgca
     4861 tgaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat tgcgcggttg
     4921 ataacggttc agacaggttt aaagaggaat aagaaaatga cagacaacac tcgtttacgc
     4981 atagctatgc agaaatccgg ccgtttaagt gatgactcac gcgaattgct ggcgcgctgt
     5041 ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga tggcagaaaa catgccgatt
     5101 gatattctgc gcgtgcgtga cgacgacatt cccggtctgg taatggacgg cgtggttgac
     5161 ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg cgcccagggt
     5221 gaagatccac gctactttac cctgcgtcgt ctggatttcg gcggctgccg actttcactg
     5281 gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct taaacggtaa acgtatcgcc
     5341 acctcttatc ctcacctgct caagcgttat ctcgaccaga aaggtatctc ttttaaatcc
     5401 tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg gactggcgga tgcgatttgc
     5461 gatctggttt ccaccggtgc cacgctggaa gctaacggcc tgcgcgaagt cgaagttatc
     5521 tatcgctcga aagcctgcct gatacaacgc gatggcgaaa tggaagaatc caaacagcaa
     5581 ctgatcgaca aactgctgac ccgtattcag ggtgttatcc aggcgcgtga atcaaaatac
     5641 atcatgatgc acgcaccgac cgaacgtctg gatgaagtca tcgccctgct gccaggtgcc
     5701 gaacgcccaa ctattctacc gctggcgggt gatcaacagc gcgtagcgat gcacatggtc
     5761 agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga aagcgctggg tgccagttca
     5821 atcctggtcc tgccgattga gaagatgatg gagtgatcgc catgagcttt aacacaatca
     5881 ttgactggaa tagctgtact gcggagcaac aacgccagct gttaatgcgc ccggcgatct
     5941 ccgcttctga aagcattacc cgcactgtta acgatattct cgataacgtg aaagcacgcg
     6001 gcgatgaggc cctgcgggaa tacagcgcga agtttgataa aaccacggtt accgcactga
     6061 aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct gagcgacgag ctaaaacagg
     6121 cgatggcggt ggcagtgaag aatattgaaa ccttccacac tgcgcaaaaa ctgccgccgg
     6181 tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt cacgcgcccg gtcgcttcag
     6241 ttgggttgta tattcctggc ggctccgccc cgctcttctc aacggtatta atgctggcga
     6301 ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg ctcaccgccg ccgattgccg
     6361 atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga cgtgtttaac gtcggcggcg
     6421 cacaggccat tgccgcactg gcgtttggta cggaatctgt gccgaaagtg gacaaaatct
     6481 tcgggccggg caacgccttt gtcaccgaag cgaaacgtca ggtgagccag cgtctggacg
     6541 gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt ggtcattgcc gacagtggcg
     6601 caacgccgga tttcgtggct tctgatttgc tttctcaggc tgaacacggc ccggactcac
     6661 aggtgatttt actgacgccc gctgctgata tggcgcgtcg cgttgccgag gctgtcgaac
     6721 gccagctggc agaactgccg cgtgctgaaa ccgcccgtca ggcactgaac gccagccgcc
     6781 tgatcgtgac taaagattta gcgcagtgcg tagagatctc caaccagtac ggcccggagc
     6841 acctgatcat ccagacccgc aacgcccgcg atctggtcga tggcattacc agtgccggtt
     6901 cggtatttct tggtgactgg tcactggaat cggcaggtga ttacgcctcc ggcaccaacc
     6961 acgttctgcc gacttacggt tacaccgcca cctgttccag cctcgggctg gcagatttcc
     7021 agaagcgcat gaccgtgcag gaactgtcga aagaaggttt ctccgcgctg gcttcgacca
     7081 ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa aaatgccgtt actttacgtg
     7141 ttaacgccct taaggagcaa gcatgagcat cgtgactatt accgatttag cgcgtgaaaa
     7201 cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaacg gcgatgtctg
     7261 gctgaacgcc aacgaatatc ccacagccgt ggaatttcag cttactcagc aaacgctcaa
     7321 ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat tacgcgcaat atgcaggcgt
     7381 aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa ggtattgaac tactgattcg
     7441 cgctttttgc gaaccgggta aagacgccat cctctactgc ccgccaacgt acggcatgta
     7501 cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca gtgccgacgc tggacaactg
     7561 gcaactggat ttacagggca tttccgacaa gctggaaggc gtaaaagtgg tctatgtttg
     7621 cagccccaac aacccgaccg ggcaactgat caatccgcag gattttcgca ctctgctgga
     7681 gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc tatatcgagt tttgcccgca
     7741 ggcatcgctg gctggctggc tggcggaata tccgcacctg gctattttgc gcacactgtc
     7801 gaaagctttt gctctggcgg gccttcgttg cggatttacg ctggcaaacg aagaagtcat
     7861 caacctgctg atgaaagtga ttgcccccta cccgctctcg acgccggttg ccgacattgc
     7921 ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt gagcgggtgg cgcaaattat
     7981 tgcagaacgc gaatgcctga ttgccgcact gaaagagatc ccctgcgtgg agcaggtttt
     8041 cgactctgaa accaactaca ttctggcgcg ctttaaagcc tccagtgcag tgtttaaatc
     8101 tttgtgggat cagggcatta tcttacgtga tcagaataaa caaccctctt taagcggctg
     8161 cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc gtcattgacg ccttacgtgc
     8221 ggagcaagtt taatgagtca gaagtatctt tttatcgatc gcgatggaac cctgattagc
     8281 gaaccgccga gtgattttca ggtggatcgt tttgacaaac tcgcctttga accgggcgtg
     8341 atcccggaac tgctgaagct gcaaaaagcg ggctacaaac tggtgatgat cactaatcag
     8401 gatggtcttg gaacacaaag tttcccgcag gcggattttg atggcccgca caacctgatg
     8461 atgcagatct tcacctcgca aggcgtgcag tttgatgaag tgctgatttg cccgcacctg
     8521 cccgccgatg aatgcgactg ccgtaagccg aaagtaaaac tggtggagcg ttatctcgct
     8581 gagcaagcga tagatcgcgc caacagttat gtgattggcg atcgcgcgac cgatattcaa
     8641 ctagcagaaa acatgggcat tactggttta cgctacgacc gcgaaaccct gaactggccg
     8701 atgattggcg agcaacttac caaacgagac cgttacgccc atgtagtgcg caacaccaaa
     8761 gagacgcaga ttgacgttca ggtgtggctg gatcgcgaag gtggcagcaa gattaacacc
     8821 ggcgttggct tctttgatca tatgctggat cagatcgcca cccacggcgg tttccgcatg
     8881 gaaatcaacg tcaaaggcga cctctatatc gacgatcacc acaccgtcga agataccggc
     8941 ctggcgctgg gtgaagcgtt aaaaattgcc ctcggcgata aacgcggtat ttgtcgcttt
     9001 ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg cactggatat ctctggtcgc
     9061 ccgcacctgg aatataaagc tgaatttacc taccagcgcg tgggcgatct cagcaccgag
     9121 atgatcgagc acttcttccg ttcgctctct tacaccatgg gcgtgaccct gcacctgaaa
     9181 accaaaggta aaaacgatca ccaccgtgta gagagcctgt tcaaagcttt tggtcgcacc
     9241 ctgcgccagg ccatccgcgt ggaaggcgat accctgccct cgtcgaaagg agtgctgtaa
     9301 tgaacgtggt gatccttgat actggctgcg ccaacctgaa ctcggtgaag tctgccattg
     9361 cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga cgtcgtgttg ctggccgata
     9421 aactattttt acccggcgtt ggcaccgcac aagcagcgat ggatcaggtg cgtgagcgcg
     9481 agctgtttga tctcatcaaa gcctgtaccc aaccggtgct aggcatctgc ttagggatgc
     9541 aactgctggg gcggcgcagc gaagagagca acggcgtcga tttgctgggc atcatcgacg
     9601 aagacgtgcc gaaaatgacc gactttggtc taccgctgcc ccatatgggc tggaaccgcg
     9661 tttacccgca ggcaggcaac cgcctgtttc aggggattga agacggcgcg tacttttact
     9721 ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat cgcccagtgt aattacggcg
     9781 aaccgttcac cgcggcggta caaaaagata acttctacgg cgtgcagttc cacccggagc
     9841 gttctggtgc cgctggcgct aagttgctga aaaacttcct ggagatgtga tgattattcc
     9901 ggcattagat ttaatcgacg gcaccgtggt gcgtctccat cagggcgatt acggcaaaca
     9961 gcgcgattac ggtaacgacc cgctgccgcg cttacaggat tacgccacgc agggtgccga
    10021 agtgttgcat ctggtggatc tgaccggggc aaaagatcca gcaaaacgtc aaatcccgtt
    10081 gattaaaacc ctggtcgcgg gcgttaacgt tccggtgcag gttggcggcg gcgtgcgtag
    10141 cgaagaagac gtggcggcgt tactggaagc gggcgttgcg cgcgtggtgg tcggctccac
    10201 cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa cgtttcggtg ccgatgcctt
    10261 agtgctggcg ctggatgtcc gtattgacga gcaaggcaac aagcaggtgg cagtcagcgg
    10321 ctggcaagag aactcgggcg tttcactgga acaactggtg gaaacctatc tgcccgtcgg
    10381 cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc acgctggcag gttctaacgt
    10441 ctctttatat gaagaagtgt gcgccagata tccgcaggtg gcatttcagt catccggcgg
    10501 cattggcgac attaacgatg ttgctgccct gcgtggcact ggcgtgcgcg gcgtaatagt
    10561 tggtcgggca ttattggaag gtaaattcac cgtgaaggag gccatcgcat gctggcaaaa
    10621 cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt acagtttcgc
    10681 aaccatgaaa tcattggcga tatcgtgccg ctggcaaaac gctacgccga agaaggtgca
    10741 gacgaactgg tgttctacga tatcaccgct tccagcgatg gccgtgtggt agataaaagc
    10801 tgggtatctc gcgtggcgga agtgatcgac attccgttct gcgtggcggg tgggattaag
    10861 tctctggaag atgccgcgaa aattctttcc tttggcgcgg ataaaatttc catcaactcg
    10921 cctgcgctgg cggatccgac gttaattact cgcctggcgg atcgttttgg cgtgcagtgt
    10981 attgtggtgg gtattgatac ctggtacgac gctgaaaccg gtaaatatca tgtgaatcaa
    11041 tataccggcg atgaaagccg cacccgcgtc actcagtggg aaacgctcga ctgggtacag
    11101 gaagtgcaaa aacgcggtgc cggagaagtc gtcctcaaca tgatgaatca ggacggcgtg
    11161 cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg aagtttgcca cgtcccgcta
    11221 attgcctccg gtggcgcggg caccatggaa cacttcctcg aagccttccg tgatgccgac
    11281 gttgacggcg cgctggcagc ttccgtattc cacaaacaaa taatcaatat tggtgaatta
    11341 aaagcgtacc tggcaacaca gggcgtggag atcaggatat gttaacagaa caacaacgtc
    11401 gcgaactgga ctgggaaaaa accgacggac ttatgccggt gattgtgcaa cacgcggtat
    11461 ccggcgaagt gttaatgctg ggctatatga acccggaagc cttagacaaa accatagaaa
    11521 gcggcaaagt cgccttcttc tcgcgcacta aacagcgact gtggaccaaa ggcgaaacgt
    11581 cgggcaattt cctcaatgtg gtgagtattg caccggactg cgacaacgac acgttactgt
    11641 tactggtgaa tcctatcgga ccaacctgcc ataaaggcac cagcagctgc ttcggcgaca
    11701 ccgctcacca gtggctgttc ctgtatcaac tggaacaact gctcgccgaa cgcaaatctg
    11761 ccgacccgga aacctcctac accgccaaac tgtatgccag cggcaccaaa cgcattgcgc
    11821 agaaagtggg cgaagaaggc gtggaaaccg cactggcagc gacagtacat gaccgctttg
    11881 agctcaccaa cgaggcgtct gatttgatgt atcacctgct ggtgctgttg caggatcagg
    11941 atctggactt aacgacggtg attgagaacc tgcgtaaacg gcatcagtga gttgcggggt
    12001 aagcggatgc gatattgttg tagcatccgg aaaaaaaaac gggcaaggtg tcaccaccct
    12061 gccccttttc tttaaaaccg aaaagattac ttcgcgttgt aattacgcag agcgttacgc
    12121 cccaagacaa tccccgcgcc aaccatgccg cccagcagca ccgccagaat caaggtaatt
    12181 gcctttttcg ggctatcgcg acgaataggt aacgtcggtt tcattacata gcggtaagca
    12241 tgaatatcaa gtttatcaac gtcaagattg tcgatatcca gcaggttttg acgagtctga
    12301 tagtagtttg gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc
    12361 gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat cttcagtctg ctgaacctgt
    12421 ggctttgtca cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc
    12481 agatctttct gctcctgcgc aactacttcc tgagttttca gagaatcctg caagtttttc
    12541 atacgcagcg cgatgttgtc tttcaggtct tttcccagtt cttcatttac ctggtcgtca
    12601 acttgctgga tatacttcgc cagctgcttc tgagcacctt caggtgtttc accaacataa
    12661 gacaccgcta aaggtaatga ctgatttttg acggtaggct caatggtcag cttttctgct
    12721 tcttcctggt tatccagcgt ttccgctaat gctgagaatg cagtgctgta acgtccgata
    12781 agtgaagcct gaatgtcgga gactttcggt acagccgatc cgtagatcac attcatcgcg
    12841 ttggtataac cagcgatttg ccccacatca ggttgagtaa cgatagctgt ggaagtccac
    12901 ttttctttgg caacggccag ataccctata gccagggcaa tagcaacaat gacagagatg
    12961 ataattgtca tcttgccacg ccacaactgc accagtaaat caatcaaatc aatctgttcc
    13021 ggatcatggt tttgcccaga aacattatta ttttctactc tcatcattac cctaacggac
    13081 gaaataattt taagtcgcca tagtttagcg ataatcgcca tgaatgctat aggataaatg
    13141 ataaaaataa tgaattatta caaagggaac ataagagaat cagggcgaaa atcgccatga
    13201 taacaggatg ttagtcactg ccaaagagat cgcgggtgta gactttgtct gccacatcct
    13261 taagctcttc tgccatacgg ttggagataa tgacgtcggc ttgtatagtc agcttagaac
    13321 agctttgatt gtaaaatgag cacgttacca gaaaaaacag ccaagtttag aactgatacc
    13381 gattatcttt ttttcttgtc taacggtata atttaacttt cagttatgcc agatgaagat
    13441 tggctatatt ctagcctgag cgaggattat aaatgaaatt tctggttacg ggagctgctg
    13501 gctttatcgg tttctatcta agtaaacggc ttcttgcagc tggtcatcag gttgtaggca
    13561 ttgacaactt aaatgattat tacgatgtca acctcaaaca agcacgactt gatttactca
    13621 agcacgacaa cttcagtttt tataaaattg acctggccga tcgtgagaaa atggcggcac
    13681 tgtttgcaga cgagcggttc gaacgcgtaa taaacctcgc tgcgcaagcc ggtgtacgtt
    13741 actctcttga aaatcccaat gcatatgcag atgcaaacct gattggattc ctgaacatac
    13801 tagaaggatg tcgccataat aatgttcagc atctacttta cgcttcctcc agttctgttt
    13861 atggcatgaa ccgcaagatg cctttctcta cagatgactc tgtagatcat cctgtttcgc
    13921 tttatgcagc aactaaaaaa gcgaatgaac tcatggccca tacatattct catttgtatg
    13981 gcttaccgac cacagggctg cgtttcttta cggtttatgg tccatgggga cgtccggata
    14041 tggcattatt taaattcact aaagccatgc tggaaggtaa aagcattgat gtttacaact
    14101 tcggcaaaat gaagcgtgac tttacttaca ttgatgatat tgccgaagct attattcgct
    14161 tacaggatgt tattccagaa aaaaacccac agtgggctgt agaaacaggc tcgcctgcaa
    14221 caagttcagc accatatcgt gtctataaca ttggtaatag ttcgcctgtg gagttgatgg
    14281 actatatcaa tgcgttagaa gaggctctgg gtattgaagc caacaaaaat atgatgcctc
    14341 tccaacccgg tgatgtactg gaaaccagtg ctgatacaaa agcactgtat gacgtaatag
    14401 gattcaaacc tgaaacgtca gttaaagaag gggtaaagaa ctttgtagaa tggtatcgta
    14461 acttctataa agtttgattt tacaaaacca taagaaaagg ccctaattta ttagggcctt
    14521 ttcttagaat gaaacaaaat aattaatcat tgccaaacaa gtcgcgcgta taaactttat
    14581 ctgctacatc agccagatcg gcagacatac ggttagaaat aataacatca gcttcttgtt
    14641 tgaacgcatc cagatcacgt accacgcgcg accggaaaaa atcgtcctct ttcatagctg
    14701 gctcataaac gattacaggc acacctttcg ccttgattcg cttcataata ccctgaatcg
    14761 aggaagcacg aaaattgtct gaaccattct tcataatcaa acgatagacg ccaacaactt
    14821 tcggtttacg tgcaaggata gaatcggcaa taaaatcttt gcgcgtgcgg ttggcgtcaa
    14881 caattgccga gatcaggtta ttcggcacag actggtaatt tgccagtaac tgcttagtat
    14941 ctttcggcag acaataacca ccataaccga atgacgggtt gttgtagtga ttaccaatac
    15001 gcgggtcaag gcatacgccc tcaataatct ggcgtgaatt aagtcccagg ctttcagcat
    15061 aactatcaag ttcattgaaa tacgctacac gcatcgccag ataagtgttc gcaaaaagtt
    15121 taatcgcctc agcctcggtt gagtcagtaa acaatgttgg tatgtcttgc ttaatggcgc
    15181 cttcctgtaa taacgcagca aaacgtttag cgcgttcaga ctgctcgcca atcacaatgc
    15241 gtgatgggtg taagttatca taaagtgctt taccttcacg caaaaactca ggcgaaaaga
    15301 tcacattttc aataccaaaa cgttctttaa tggactctgt aaaaccaaca gggatagttg
    15361 attttataat cattaccgcg ttgggattaa tttctgtcac atcacgaatg accgcttcca
    15421 cgcttgaggt attaaaataa tttgttttcg gatcataatc ggtaggtgtg gcaataataa
    15481 cgtaatcggc atttttatac gcgtcatact tatctgtcgt agcgcggaaa ttgagatctt
    15541 tagtcgccag atactcttca atctccttat caacaagcgg tgactgcctc ttgttaagca
    15601 tgtccacttt ggcctgaacg atatccagtg caaccacttc gtggttttgc gcaatcagaa
    15661 taccatttga aagaccaaca taacctgttc ctgaaattgt tattttcatt agctctgact
    15721 tcttccggtt aaacatttag agtggtcatt aatccaccac cacagcttca tctactgcgg
    15781 gatttttaac gctgatgtag atgaactgtc aaggcagcga tcctgctgtg cggcgctgta
    15841 ttatatcgcg tttttaacta taaattataa aaaaaggccc taacctgccg ctttgtataa
    15901 taaaaaagcc cggagggttt ctccgggcct tgttttgatt aattgattta aatcagatta
    15961 atccagccat tcggtatgga acacaccttc tttatcaatg cgcttataag tatgcgcacc
    16021 gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga acagcggcgc ggtagctgtc
    16081 gtaataggca accgcagcgg cgaaggtcgg caccgggata ccgttctgta ctgcgtaagc
    16141 gacgacatcg cgcagcgcct gctggtagtc atcggcaatt tgcttgaagt aaggagccag
    16201 caacaggtta gcgatctgcg gattttcggc ataagcatcg gtgattttct gcaggaactg
    16261 cgcacggatg atgcagccag cacggaaaat cttcgcgatt tcaccgtagt tcagatccca
    16321 gttgtactct tcagacgcag cgcgtagctg agagaagccc tgagcgtaag aaacgatttt
    16381 gcccagatac agcgcacggc gaactttttc gatgaactca gcattgtcgc cagctggctg
    16441 cgcttgcggg ccagagagaa ctttagatgc ggcaacacgc tgctctttca gagaagagat
    16501 ataacgtgca aacacagact cggtaatcag cgacagcggt tcgccgagat ccagcgcgct
    16561 ctggctggtc catttgcccg tacctttgtt tgctgcttca tccagaatca catcaaccag
    16621 gtagttaccc tcttcatctt ttttggtgaa gatatctttg gtgatgtcga tcaggtagct
    16681 gctcagttca ccgttattcc actcggtaaa ggtctgcgcc agttcttcgt tggtgaggtt
    16741 caagccacct ttaagcagag aataggcttc agcaatcagc tgcatatcac cgtattcaat
    16801 accgttgtga accatcttca cataatgacc tgcaccatcg gcaccaatat aggtaacgca
    16861 cggttcgccg tcttcagcca cagcggcgat tttggtcagg atcggcgcaa tcagttcata
    16921 agcttctttc tgcccaccag gcataatgga aggacctttc agcgcacctt cttcaccacc
    16981 ggaaacaccg gtaccgataa agttaaagcc ttctgcagaa agctcacggt tacgacgaat
    17041 ggtgtcatgg aagaaggtgt taccaccatc aatgatgatg tcacctttat cgaggtatgg
    17101 cttgagggaa tcaatagcag catccgtgcc agcacctgct ttcaccatta acaggatgcg
    17161 acgaggcgtt tccagagatt caacaaattc tttcaccgta tagtaaggaa ccagtttctt
    17221 gcctggattt tcggtaatca cttcttcggt cttttcacgg gaacggttga aaatagagac
    17281 ggtataacca cggctttcga tattgagcgc aaggttgcgc cccatcactg ccataccgac
    17341 gacgccgatc tgttgctttg acattgttta ctcctgtcag gataccgctg ggtggtatgc
    17401 gggttatgct taattataga atatgcctaa taaaaataaa tccataacac ttaatcagaa
    17461 aattattatt atcgattcct aacgattgaa tacatcagct cctttaattt agatggcatt
    17521 atacgaaaaa atgttctcaa catagcatta cttattaatt cattttttcg aataaaacca
    17581 attttatatt gataatacaa tactttatac tcgtacaata aatatgacaa tccacgtcga
    17641 gccataagat tacgaccagt tcgcattttt aataaaatat ctggaagatt tgcaaatctt
    17701 gcattatgta caattaatag gctccacaat gcaaaatctt gagattttct gaatggagga
    17761 taaccaccaa cagctaatac tgtattcttt ctaaaaatta cagaaggatg gctaactgcg
    17821 cttcgtttcc tcgcgaattt aactatttct ctatgttcga gaggcacttt gcgtgttgaa
    17881 ataaactcct cagtaacagt ttcaatttca tcaataaaac tgccacatac atctatttct
    17941 gaattattaa tcataaaaga aatttgtttc tcaaaccgat gaggcaaaga aatatcatca
    18001 gcatccattc ttgccactaa ctcattccta caagccttta atccttcatt taaggcatta
    18061 gccaatccaa catttctagg taaaggtaca aatgttacta ttttattgcc aacatcatca
    18121 atgaatgaat ttataatatc gatgtgtgtt tgatggagtt ctccatctgc aacaattact
    18181 atttgatctg gcttaagtgt ttgatcgtga aaaatagagc gtagagccac ctcaaaaaat
    18241 tgcggtagat catttttata aatgctaatt aaaactgaga atttttctaa tctatgattc
    18301 atttcatttt accacttcga cccattaaac cgtcattaat gccttttaaa aaaaaatata
    18361 accttttatt accatttgga aggaaaatag gatataaaaa aacctttcca attaatttaa
    18421 ccagactaga aattttccag tagatgggta cataattttt atttaataaa agaaagatat
    18481 ttcgagtagc ataataatga cgaaatgggc ttggcaaacc gacagaaaga atatttaaga
    18541 tcttaaatcg cccatctcca agtctatgtg caagtaacgc atttttattc ctaattactt
    18601 taaacccagc agctcttaat ctccaacaat attcatggtc taccgcatcg ataaaaagct
    18661 catctttcat tcctccaaca atcaaccaac tattttttgg tattagactg ccagaactta
    18721 atgtactatc tacctcataa taaacttctg taagtggttt cccttttttt acccttgctt
    18781 tatttaattc accagttact ttatcaaaat cttgtgaacc aactaaacca acattgacat
    18841 tttgtttaag caattttttg taacaagtaa gtaactgctc taccatctta ggatcaggaa
    18901 tactatcctg atccatttgc aatataaaat cagcgccatt ttcaaaagcc catttcattc
    18961 ctatactttg ggcttctgct atgcctaaat tatcattgaa attgaatatt tttacatcgc
    19021 ctgaagaatt ttcagcatat ttataaccat ttgtagagtt attgcaaacg acaactttag
    19081 taacttgtct caacaataat tcaaccgcat tttttaaatc attatgttct gggttgtaag
    19141 caaccaaaac ggcatataca gtgtccatct tcaccttaaa accttcattt agctttcatc
    19201 ttttttagaa cattacttaa tgtcactaat acaattatta cagcaacatg gttagagtct
    19261 aaaatataag gattagtaat tgcataagaa acatatagaa aatatagcac acacaactca
    19321 ctgtatttta tgattttaat cgtgagaagg agattaatta ataaaaacaa agtaaataaa
    19381 ataacgccaa gttgatttaa aaaataaact gactgcaatt cataatatat atatgcacta
    19441 taatcacgga taggagtttg aattttgatg acattaccca aaccagaacc tataacaaaa
    19501 tttgatacag actctgtaag atcattaatt aatacagtaa actgatccca tctaactcct
    19561 aaagaagaat cagctccatt tgatttcatg attatcaact caattgaata tgtaataaaa
    19621 aaagggagaa tcacagtaag aaaaacccca aaaataattt tccttaattt agcgtatcgt
    19681 gagttagatt tagaacatag tataatatac ataaaaaaca agcatatcga aacaaaatat
    19741 gcaaaattac cagccactat agtacctata gccagaataa cggttattgt atttttgaat
    19801 cgataataga aataatcttt tatgactata tgcaacataa aggcaaatgg aatgagagca
    19861 tttcctttaa tttgaactct atagaaacca cttccatatg tataaacatc accataatca
    19921 ttctccaaaa aataatgtct tagtgctgaa taatcaccaa taccatatgt ttttgtcata
    19981 taaatactaa tgatggatat aataaccgcc tgtaatacca ttaaatataa aaatatttta
    20041 acaatcgaga tggttccata agagcagaaa taagcacata atataaataa tatgataata
    20101 taaaacctaa ttattatcgc tatatcgtta cccttgatat aggaataaat aaaatttata
    20161 aaaagagcta atagaaatat taaaataaca ggatagtgat atattccgtt tgcaattttc
    20221 tttgtaaatg acatgataca aagacataaa aacccctcca taatccaact atattgaata
    20281 aatggaaagc tacgtgtaag gaaaaatata aacccaaaaa acaaaagaac acttaaactt
    20341 ttgtcttttg agttataaaa atcagaagtc atgtttgcac tctaattaga tgggcttgag
    20401 gaagtaatcc ctaaaatcaa ttcgctatta atatttcgta tcaattaata ataatatcaa
    20461 aaaatctaac gatgttctta cagaccatgc tattgcggct ccaacaattc cccaatgata
    20521 aataaaaata tataatatgc ataaatatgg gataacttcg agcaaatgaa taatagctgt
    20581 aatttttgat cttccactag cctgaactga aacaaatggg atttgtgcaa tgcaattaaa
    20641 aaagaaacct attgcaagaa tttttaatac tatacctggc gtcccatgat atgtaggtcc
    20701 catccaagcg gacattataa aatctgataa aataattatc aacattacaa ttggaagtat
    20761 accaataacc attataaaat atgataatat tttagtttgc tttaccgatt gcaattctga
    20821 acttaatctt ggaaaaatag ctctggacaa cgcacttggt aatatcgtta agcgttgtat
    20881 accttcagac ggagcagtat aaaaagaaac tttatcagcc cccacaatgt gtgaaagaat
    20941 aaaacgatcc atatatgtca taatagggct aataatattg ctaactgtta tccagcttcc
    21001 aaagccgatt aatcttttaa ctgttacaat ttttacagac agcccagatg atattattag
    21061 ttttcgacta aatataaagg tcactataag tgataagact cttgccataa ctaaaccata
    21121 tatagcactt agtaatcctc catgaaaaaa acagaaaatc actggtaatc cagccacaaa
    21181 agagttgtta attgatttta ttaaatttac ttttctgaac ttttccatcc cctcaaaaat
    21241 ccccaaccag acttggttta acaagtataa gggtatggta gctgaaataa tatatattgc
    21301 tttgacagat tctacaacat gattcgcgtt aatgtttaat aatttaacaa ttacattgct
    21361 actcaaaaat agtacactac cgccaatcaa gcccaatata gttagaatta ccgttgaagt
    21421 tgaaatgatc gctcttaatt ctttatgaac atttttatat attgatactt ctcttataac
    21481 agctctggtc aatccagcat caaaaatact tgcatatcca actaaggcaa tagctaacgt
    21541 aaaaaggcca aattgctcgg tccctagaat tctagacagt atacctaacg caggaattgc
    21601 tattaatgat ggtataatat acccacttat attccataaa gtattcttta caatactcac
    21661 aaaaataatt ccttcatgtt atgcaattct ttagcccttg catctttaat cgataaaata
    21721 taattattat gttctatcgt cggccatttt atgctcagaa taggatcatt ccatacaatc
    21781 cctctatcac tatcaggatg ataatagttc gtcgttttat ataaaaattc cgcagtctcg
    21841 ctcagcacca aaaaaccatg tgcaaatccc tcagggatcc acaattgccg cttattctca
    21901 gcagataaat tcaccccaac ccatttacca aaggtaggcg acgatttacg aatatcaaca
    21961 gctacatcaa aaacctcacc aacaacgcaa cgtaccagtt tcccttgcgc ataaggttct
    22021 aactgataat gcagcccgcg taaaacacct ttactagact tcgaatggtt atcctgaaca
    22081 aattcaacct tacgtcctac agcttcttcg aaaactttct gattaaagct ttccataaag
    22141 aaaccacgct catcaccaaa aactttcggc tcgaaaatta acacatcagg aatttctgtt
    22201 ttaattacgt tcattttatt aataaccttt aatcattttc agcagatact gtccataagc
    22261 attttttttc agcggctccg ctaatgcttt cacctgttca gcatcaataa accctttacg
    22321 gtaagcaatt tcttctgggc aggaaacctt tagtccctgg cgctcttcaa tggtggcaat
    22381 gaagttgctt gcttcaataa gactctgatg tgtccccgta tccagccatg cataaccacg
    22441 ccccatcatg gcaacggata aacgcccctg ttccatataa atacggttaa tatcggtaat
    22501 ttccagttca ccacgggcag aaggcttaag gtttttcgcc atttcgacaa cgtcgttatc
    22561 atagaaataa agcccggtta ccgcataatt actttttggt tgtagcggtt tttcttccag
    22621 gcttattgcc gtaccgtttt tatcaaactc aacgacgccg tagcgttcag gatcattaac
    22681 gtgataggca aataccgttg caccactttc tttgttaaca gcgacatcca ttaacttcgg
    22741 cagatcatga ccgtagaaga tattatcacc aagaaccaaa gcacaatcat caccaccgat
    22801 aaactcttca ccgataataa acgcctgcgc aagcccatct ggagtcggtt gcactttgta
    22861 ctgaagattt agcccccact ggctaccgtc acctagcagt tgttgaaaac gaggagtatc
    22921 ctgtggcgta ctaataatca gaatatcgcg aatacccgcc aacatcagtg tagagagcgg
    22981 gtaatagatc atcggcttat cataaatagg taatagctgt ttactgacag ccatagtcac
    23041 aggataaaga cgtgtaccag aaccacccgc taaaataata cctttacgcg ttttcatttc
    23101 atcattcctt ttaattcatc ttgctccacc atcacgaaca agatgcaaaa actattaaat
    23161 tgctgtagtc gtaattaatt cgttgagcat tcgtttcaca ccaacctgcc agtcaggcaa
    23221 gacaagcgca aagttctgct gaaatttttc tgtattaagg cgagagttat gtggacgacg
    23281 agctggtgta ggataggctg ttgttggtac tgcgttgagc ttgttgagtg caaggggaat
    23341 acctgctttg cgcgcctctt caaaaaccag cgcagcataa tcgtgccagg ttgtggtacc
    23401 actggctacc agatggtaca aacctgcgac ttccggttta ttcagtgcca cacgaatagc
    23461 atgtgccgta caatcagcca gcagctcagc acctgttggc gcaccaaatt gatcatttat
    23521 cacagccagt tcttcgcgct cttttgccag acgcaacatc gttttggcga agttatttcc
    23581 tttagctgcg tatacccagc tggtacggaa aataagatgc ttcgcgcaat gttcctgtaa
    23641 cgctttttct ccggctaact tggtttcacc gtaaacattt agcggtgcgg ttgcatccgt
    23701 ctccagccat ggcgtgtcgc catttccagg gaatacgtag tcagttgagt aatgaattac
    23761 ccaagcccca acttcattag cctcttttgc aattgattca acactagtcg cattgagtaa
    23821 ttgtgcaaat tcgggttctg actcagcctt atctactgcg gtgtgagccg cagcattaac
    23881 aataacatca ggtcgaattc ttttgactgt ttcagctaca ccttcaggat tactaaaatc
    23941 accacaataa tcagtggagt gaacatcaag agcaatcaaa ttacccaaag gtgccagagc
    24001 acgctgtagt tcccaaccta cctgccctgt tttgccgaaa aggaggatat tcattactgg
    24061 cggccctcat agttctgttc aatccacgat tgataagcac cacttttcac attatcaacc
    24121 cattttgtat tggacaggta ccattccaat gtcttccgaa tcccgctctc aaacgtttcc
    24181 tgcggtttcc agcccaattc gcggctaatc ttctctgcat caatcgcata acggcgatcg
    24241 tgtcccgggc gatcggcaac ataagtaatt tgctcgcggt aagatttctc tttcggtaca
    24301 atctcatcca gcaaatcaca aatagtgagc actacatcga tgtttttctt ttcgttgtgt
    24361 ccaccaatgt tataagtttc acccgcttta ccttcggtta cgacggtata taacgcacgc
    24421 gcatgatctt caacatacag ccagtcacga atttgatccc ctttgccata aataggtaat
    24481 gccttacctt ccagagcatt cagaataacc aatggaatca atttttccgg gaaatgataa
    24541 ggaccataat tattagagca attagtcaca atggttggta aaccataggt acgtttccac
    24601 gcgcggacta aatgatcgct ggatgctttt gaagcggaat aagggctgct tggcgcgtaa
    24661 gctgttgtct ctgtaaataa gggtaattct tctgtattat ttacctcgtc aggatgaggc
    24721 aaatcaccat agacttcgtc agtagaaata tgatgaaaac ggaatctagt tttcttgtcg
    24781 ctatcaagag cagaccaata attgcgagcg gcttccaaaa ggacatatgt accaacaata
    24841 ttggtttcaa taaatgccgc aggacctgta attgaacggt caacatggct ttcagcagcc
    24901 aggtgcatca ctgcatctgg ctgatgctga gcaaaaatcc gtgccattgc agctgcatcg
    24961 caaatatccg catgttcaaa aacatagcgt tcagaatcag aaacatcagc aagtgattcc
    25021 aggtttccgg cgtacgttaa tttatcgaca ttaacaacac tatcctgcgt attatttata
    25081 atgtgacgaa ctacagcaga accaataaat cctgcgccac cagtaacaag tattttcacc
    25141 taatttattc catattgctt cagagcatgc tgtgaaataa gcggctctca gtttgattaa
    25201 tagaagtatt aatgcacgct accgcccctg gctttacagc taccagagca ctgcatgcat
    25261 gcctacgatg tgacgagcgt tacccactcg cgctaaaccc gaaaaattca aaagctaatt
    25321 gtcttaccaa tccgctctgg aaacaaggaa aatcctggaa aactttgact aaaatcctat
    25381 tgctaactcg ttgttattct gattgtttat ataaaacaac ggcaggaata ttcgcaacaa
    25441 attactttca ccacgaatct tcactgccgt tataattttc ttatcaaccg ttacatccgg
    25501 tcagattttc attattcgct taacagcttc tcaatacctt tacggaactt cgccccttct
    25561 ttcaggttgc gcagcccata cttcacaaac gcctgcatat agcccatttt tttaccgcag
    25621 tcgtagctgt cgccggtcat cagcattgca tcaacggact gttttttcgc cagctcggca
    25681 atggcatcag tcagctgaat acgtccccat gcaccaggct gagtacgttc aagttccggc
    25741 caaatatcgg cagaaagcac atagcgacca acggccatga tgtctgagtc cagcgtctgc
    25801 ggctgatccg gtttttcgat aaattcaaca atgcggctga ctttaccttc gcgatccagc
    25861 ggttctttgg tctggatgac ggagtattca gagaggtcac ccggcatacg ttttgccagc
    25921 acctggctac ggcccgtttc attgaagcgc gcaatcatgg cagcaaggtt gtagcgtagc
    25981 gggtcggcgc tggcgtcgtc gatcacaacg tctggcagca ccacgacaaa tggattgtca
    26041 ccaatggcgg gtcgtgcaca caaaatggag tgacctaaac ctaaaggttc gccctgacgc
    26101 acgttcataa tagtcacgcc cggcgggcag atagattgca cttccgccag tagttgacgc
    26161 ttcacgcgct gctcaaggag agattctaat tcataagagg tgtcgaagtg gttttcgacc
    26221 gcgttcttgg acgcatgagt taccaggagg atttctttga tccctgcagc cacaatctcg
    26281 tcaacaatgt actgaatcat tggcttgtcg acgatcggta gcatctcttt gggtatcgcc
    26341 ttagtggcag gcaacatatg catcccaaga cccgctaccg gtataactgc ttttaaattc
    26401 gtcattattt tcctacctct aaggggctga tagtgcgtaa attattgtca taggttagcc
    26461 aaacggtatg gctatatacc aagcataact ttgattaaac cttacgataa cactacacac
    26521 catcagcatc tgggttactc ggattactcg gaaatccaca tactgataat ttaatcagta
    26581 cctctttccg aataatcgta gtccaacctg gtcctttttt ctctgactcg tctgcattac
    26641 tcagaaacaa acgttatgtc gtcttttttg gcatggacga attcatactg cagagttcga
    26701 tccagacctt gcgacagcgt atacggtgca acaaaacctg aagaatgcac tttcgttgcg
    26761 tcaaactgtg ttgttgcgca gaattttttc acgcgcacag agctgacagc gtattttttg
    26821 cccgtaattt tgctcaggat atcaaagcaa tatccaccca gcattcctag tgggtaaggc
    26881 aagtgcatag aagggatctt tttgttcagg ctttgttcaa cttcagcaac caactggttc
    26941 atgttcaggt ctggcttatc aacatagtta taaacctcat aacctgcggc aacattcttc
    27001 agtttgtact tgataaactc aacaatgttt ccaacataag ccatggactt atagttagtc
    27061 cctgcgccca ccatcataaa cttgccgcca gcgatctgtt tcagcaagtt atagacgtta
    27121 ccgcggttgc gttcaccgaa gataacggta ggacggatga tggttaatga acgttctgtt
    27181 ggtgctttgt tataccattc acgcagcact tcctctgcct gccacttact tttgccgtag
    27241 tggttgaaag ggtcgtgtgg atggttttcg tcagggttgt gtttgttcaa accataaaca
    27301 gcaacggaac tggtaaagat gatattttta acgccatttt tttccatggc cgccagcaca
    27361 ttgcgggtac cctgaacgtt gacatcataa tagagagaag tagggctgac gtcatcgcgg
    27421 tgttccgctg ccagtagtac aacagtgtca aaaccggcta acgcctggtc gagtgcctgt
    27481 tgatcacgaa catcaccaat ctgtgtgatt tctggataaa agtggctctg ccgtttgtcc
    27541 aggttcttga tattaaagtc agcaattgcc gtttcaagta gtcgggttcc tacgaatccg
    27601 gaagctccta tgagcaaaac gttattgttc ataaatcact ttagtctggt tgttacgtaa
    27661 gaaacacaag ataaagatga gtaccttccc tgagtagtca atgctgccca gccccagctt
    27721 taacagttag tgtgaggatt ataatctttt agaacattat atccagtaag tttatgaatg
    27781 gtcgcaaatc tactctctcc gttccggcaa tctaaagtta atcttttcca cattaacaat
    27841 atggtgatta atcctgtcga tgtcgacgga gctttgccct ctttcattca tcgcatgaac
    27901 atttgcaagt gacagcagtg tttctttttt cgccataaag acaccgcgta cgtctttgcg
    27961 catgtcgaag ttcatgctca atgctggtcc aactgaggat tcctgcatca ccttgatatt
    28021 acgcataaaa agatgttgcg gtttgttgtg taactccagc gaggcacgct tcatctcaat
    28081 gttagtcagt gccacaaagg agacagcatt cccggcggag atttggatgc cgcgcaattt
    28141 ataaacaaga tgggtgttat ccagttgaat attattcact cggaaatttt gcggtatcga
    28201 gagatatttg cctttaatta ccccatagcc gattaacatc ccggcgctat taatcatttc
    28261 aatattatca atcacgaaat tgtcacaacc gtaaatagca actgtcgcgt tatcaatgcc
    28321 tgctttctta ctgaaatccg gcgtgatatt gcgggctttg atattacgga taacaaaatg
    28381 tttgccattt tcaacatgga tcaactgccg acaatccgat cccgtgatat tcgccacgac
    28441 aaagtttttc actgcctggt cttccgggta gttattatcg taggtactcc ccgcaaggcc
    28501 tatgccaatt ccccagttga ttttgccatt agtacagttg atgcgctcga tgacatggtc
    28561 ggagatcaaa atatcactgt cgttaattgc cacgttccat tcaatggcgt cgccctgtaa
    28621 gtcgctgaac ttacaattgg tgatgttggc accgataatc tggttatgaa atccctggcg
    28681 taagatggcg taattagcgt ggctgacagt gaggttatcg atggtcaggt tgctcatgac
    28741 ccgtttgttt ttgccgccga tataaatctg cgttaccggg ccaaagccgc tcatcgccag
    28801 ccctttgatg gtgcagtcag aaccacgcac atccaaggtg atgttatgca tactgccgcc
    28861 ctcctcccct gtcacctggc tgccgtcctg taagacaaat cgccctctgc cgttgccgcg
    28921 caagcttcca agaatgtgta acgttttacc gggagggata aagatgccgg tgttgatatt
    28981 gtcacaaacc aatccggcag gcacgacgac cgtatgccct tcgctgaagg cttgtttaaa
    29041 tgaggcgatc cagtcgtgtg ggttgtagtc gttaatgtta acgctttgtc gggcgggaag
    29101 tgcgcgagcg aaaggggtat ggaggaaggc aagcgccgag cttgccgtca ggaaggtgcg
    29161 tcgggagagt gttttaaatg gcatgcgttc tcctctataa agcttgcagc aagctggcga
    29221 gttctcgatt gatcacctgc tggttaaaat cgtgttcgac tttttcgcgc gcacgtttga
    29281 caaccggagc cagttcgtcg gtgcccagtt ggctaaacgc cgccagtcgt tgcgccagtg
    29341 cgcgagcatc gttctcaggc accagccagc cggatttgtc agcctccacc agttccggta
    29401 ttccgctatg cagagtagaa accaccggaa taccgaccgc catcgcttcc attagcgcca
    29461 ccggaatgcc ttccatatca ccatccgcgc ccgttaccga tggcaacaga aagacatccg
    29521 cgtcgtcgag catcgctttc acttcgtggc tcggtttaaa gcccggcatc tctaccacat
    29581 cttccagttg atattgttcg atgagggtac gcaggcgtcg ttcccacggg ccaatgccga
    29641 ggatgcgata gcgaaatgcc acgccctgct ctttcaactg acggcaggct tcaatcgcca
    29701 catgcaggcc ttttttctcg gttaagcgtg cgacggaaat gatttccagc ggcgttgcgg
    29761 gcgctttcac cggacgcggg ctaaagcgcg tcatgtccac gcccatacgc gatacggcga
    29821 ttttttccct cgggcagccc attttttgca gccttccggc ccacaaatcg cttatcggta
    29881 acatcaggtc gccacggcga aacagttgtt gatattcggg agtgtaatgg ttgagcactt
    29941 cccgactgga aatatcaatg ccgtggaaaa tggtggcaat tttgccgcga atgacaccca
    30001 gttcccgtag ttttgctgcg gttacccccg ccgggccaaa atgagcgatg aacacatcgg
    30061 catgaagcgg tgttgcgacc tggccgcaaa tggcagacaa aatcaggttc cgcgactcgg
    30121 caccatagcg tttgaggttg agcgcctgcc aggtattttt acgataaatg ccgcgtaacg
    30181 tctggctggc gcggtggcgc agtttcgcta ttttgcccgt aggttcgtcc tgtaaccagt
    30241 gggttctggc ggcaaggttg tatttcgtcc atgccgcgtg ggtgttttgt gtgtcgcctt
    30301 tttgcagcgc gagaatctct acctcaaatc ccatatcaat aaacgcggta atttggttaa
    30361 ggacgaaggt ttctgacgac agcgggaatt tcagtaaaaa gaagccgacc ttcatttcac
    30421 ctccccgatg cgctcaagca cagactgcac catctgcatt cctgtctgac gctcacgact
    30481 gacggcttca ctaagtcgcg cattcagtgc cggaagctgg cctaaggtat ccgcaaccat
    30541 cgcttgcagg ctgccgtcta ataaatgacg gatatcaatt gccatctccg gtagtcccag
    30601 ctgctgcata atcccggcgg atttatgttc gtagttgatg gcaattgccg gagtggcaaa
    30661 attcatcgag ataatggcag agtgcaggcg cgtaccgacg gtgagttcac aggcaccgag
    30721 aattttgccc atttccagat cgttgagttc atccatcacc acatggtaac gggcaggatc
    30781 gctgatgtgc tggcgaagat tgagcgccat catgcggtcg tctttgttat agctgtcgat
    30841 acccgtacag gtggagagtg caatgacctg atagccttcg tcgagaatgc gattgaccac
    30901 cccggcaaag gctttttcat acgcttgttg agtggtgccg aggcgtttgt caaacggtgc
    30961 cagttcgcgc agggtaattg ctaccgtttt ctgttgtgcg gcaacgtcca gccagtgttg
    31021 aacagcatag ctggcggtga agtcttcggt gtggtgatcg accagccacg cggtatcgac
    31081 gccatgttcc acttttgcgg tagtgatatt gctgcgtttc atcagatcaa gactgaccga
    31141 ttcgcgcagg atcagcgcgt cacagtgacc aaaaacgtag ttcgccagtt ggttaaattg
    31201 ctcatcctgg aaggggccga cgctgtgacc aatcataaac agcggctttt tcgccataaa
    31261 cgtgcaaagc gcatgttcaa actgcggcac gccgtagaga tcgacaaaaa acgatcctcc
    31321 gacctgaata atggcgtcgt aacctgagag caggcgcacg aagtcggtga atccctgggc
    31381 gatggcgata ttacgcagct tgccagtgtc ggtgacgcgt gagagcaata cctggtgttg
    31441 atagcggcga cggaggactt ttttaacgcg cccgacaacg cccgccgcgc tgttgtgttg
    31501 tttcatttgc aggaacagag gatcgcccat taccgggcgg ttgagtagcc aggaagaact
    31561 gaccggatag cggctcatca cgtccacttc ggcttgtgga ttgaggatgt tgatggcatc
    31621 aagtaagccg cgcaagatgg cgctgtcgcc acgattgccg caagtgtggt tgcccagaat
    31681 aagtaatttc attttttcct cataaatttg atgccaagtg aggctgtgtt tgttttgttc
    31741 gttgccgctt tttgcctgat gcgacgctga cgcgtcttat caggcctaca agacccgagc
    31801 acagaaccgt aggccggata aggcgttcac gccgcatccg gcattcagtg cctgatgcga
    31861 cgctgacgcg tcttatcagg cctacaggtc ccgagcacag aaccgtagga tggataaggc
    31921 gttttacgcc gcatccggca accgttgtcg gaaccgaaaa cagcaactca ccccgcccgt
    31981 aaaagcattt tcattttttc gctgcgacaa aactgacgct tcacttccac caccagcgga
    32041 tggcgcgaca gcacaatcat caccacaaac gccagcacgc ccgcggctat ttgcaccgcc
    32101 agcagcatcc ccagcgccag ttgccctttc agcacaatgc ccagcgcata actgaccacc
    32161 agcgtcggca acgagagata aaacggcagc cacaaactca ggatgtactg gcgataactg
    32221 gaaccaagaa ccggtttaat catcacgaag taactcagaa tggtgttgat aatctgcacc
    32281 agcaggaaac cgagcgtgac gccgattgcg cccgccatct gcccaccaat aacaatcgcc
    32341 ggaataaaca gaaatgtttt gaatacgttg aatttaaagc tgatatcgac ccgcgctttc
    32401 gccatcagca gcgaaccaat cgggttaccc acggagcgca gtagccccac cacacacaga
    32461 aattgcagca ccggaataat gctgttccac ttctcaccaa agaccagtgg tacaaagtta
    32521 ttcgacacca ccattagccc gagcagcgcc ggaaagttga taatccccac caccgacagc
    32581 ggtttgtaga agttaatacg cagcttttcg gtgtcgtcct gaattttggc aaacgccgga
    32641 aacaacacac gggtgatgat tgggttcagc ttcatcggcg gcacaacggc cacgttgtaa
    32701 gccaggttgt agccccctgc cacgcccgcg ccgagaatac gcgccagcac cagcgttgaa
    32761 aggttggtat tgagatagtt gatgatgctg tccgccgtca gccaggcacc aaagcgtaag
    32821 ttcggtgcca ccgacgccag cgagaaatgc agaccggggc gataaatttt gcggccaaag
    32881 tagccaaaca gcagcgttct caccgcacta ttgaccagat aaccgaggat cgcggtcatc
    32941 gccagcggcc agaaatgggc gctaaccacc gtaaaagtga agcccgccag taccgcgctg
    33001 gtttcgatca tgccgatttt gttgaactcc agctcctttt gcatcaacgc gcggaactgc
    33061 tgcccctggg ggatcaccac aaacgccagc gacaatgttt taatcaacgg tgccaggtcc
    33121 gggttattca gcacgtcgcc gatgagatca ctcaacaaaa acaccgccag acacaccaca
    33181 agccccagcc cgacgttcag ccagtacagc gtggtgagtt caaggtggct gatttctttt
    33241 cgctgaataa tcgagttagc gataccgaag tcagaaagcg tatctgccag cgcgatgatc
    33301 accagcgaca cagtaagcaa accgaactgg tggttgtcga taatccgcgc cagcacggtc
    33361 atctgcacca gcccgaggcc gatgatgatc accgtggcaa tcgccgacca cttcgcgccg
    33421 ctgatggttt tttcacgtaa gctcatatca atatgccgct ttgttaacga aacctttgaa
    33481 caccgtcagg aaaacgattt tgatatcgaa ccagacgctc cattcacgga tgtactcaag
    33541 gtcgaactcg acgcgttttt ccattttctc cagcgtgtcg gtttcgccgc gccagccgtt
    33601 aatctgcgcc cagccggtaa tgcacggttt cactttatgg cgcagcatgt agccttcaat
    33661 gagctggcga tactgttcgt tatgcgctac tgcgtgcgga cgtggaccga caatcgacat
    33721 ccccccggtc agcacattga taaactgcgg taattcatcc agcgaggtgc ggcgcagaaa
    33781 gttccccact ttagtgacgc gcggatcgtt ctgcgtcgcc tgggtcacca ctttgtcgtt
    33841 ctccatcact ttcatggaac ggaacttcca cactttgatc ggcttgccat ccatgccgta
    33901 gcgagtctgg cggaaaataa ccggccctgg tgaactgagt ttcaccgcca gcgcaataca
    33961 gcacagcacc ggggagatca gcagcaagat cagcgtcgcc agcacaatgt cttccgcacg
    34021 ttttagcagg cggttaaccc cggaaagcgg cgtgtcatac agcggcacca ccggaacgcc
    34081 gttcatctct tcgagacgtg aatggagaat attgaaggta aagacatcgg ggatcagcag
    34141 caccgaacag gtggtgtccg ccagttgatg gaccagtttt ttcactcgcg cgccgtcgca
    34201 catttgcatc gcgatataga cgttatgaat cttacccgct ttagcatcct cgaccagctg
    34261 ttgcagattg cccgcccagt cgttagaaac accgcccggt ttcgggtcgt ggtaaacgcc
    34321 caccacttca aaccctaacc acggctgatt acggaagctc tccatcagca tttgtccggc
    34381 ggctaaatcc cccgccaccg cgaccatgcg cttgttatag ccatgattac gcagccagcc
    34441 cgccccaatg cgaatacatg aacggcaaac caccagtccg atactggtca gcccatacca
    34501 cgccagccag attttcagtt gcgtgtcgaa atcattgttg aacgccacca gtccggcgct
    34561 gaaaatcacg cttaaggtcc agttttgtag caacagggca aattctgtcg ctgcccgaac
    34621 accgcgccat gagcgataaa aatcggtgat gccgcccagc atctggaaca ccaccagcgt
    34681 aatcaacgcc accaacaggt gcatgtagag gaatgacagt ccgctcactt cgcaaaccag
    34741 ccatagtccg gcaaacatga tggtgatatc tgaaaagcgt tgcaccatag agattaacga
    34801 tgcattggtt ttcgctcgcg cttttttaga tttgtcatcg ttgttcctgt tattagcccc
    34861 ttacccgcag tggggtaagg gaagatccga cattactcgt tcagcaacgt cagcagagtt
    34921 cgcgttcgcg cttccatcag cggcacatca ccgcgtgatt ccacattcaa ccgcaccacc
    34981 ggttcggtgt tggaggagcg cagattaaag cgccagtcgg caaaggtcat gctgatgcca
    35041 tcggtgcgat ccaccgccag cgcatcacgg ctaaaatgct gttccacgcg gttaatcgcc
    35101 tcaacgggtt gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg
    35161 tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc
    35221 cacgggatca tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt
    35281 tcgccaccgt agatggcgtc ttccttgcgc atacgttctt taataaaggc gtgtccggtt
    35341 ttcgacatca ccgacgtgcc gccagcggca gtcaccacat caacggtatt ccaggagaga
    35401 cgtggatcgt ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg
    35461 ccgacaatgt agtagccttc gataaactgc cctttttcgt caaacaggaa gcagcggtca
    35521 aaatcgccat caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg
    35581 tcgtcgcggc attccggcag tagcgggtta ggaataccgt tggggaaatt gccgtccggc
    35641 gtgttgtgca ctttgattaa ttccacgggt gcgccaaggg ctttaaagcg ggcttcaatg
    35701 gcgtccacca ccggacccgc tgcgccattc ccggagttaa tcaccagctt gagcggcgtg
    35761 aggtttttga cattgatata accgaacagg tgatcaacgt aagcgtcacg caggttgatt
    35821 tgctgatagc gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc ttcagccaga
    35881 cgctggacgt cgcgcagtcc ggtatcaccg ctgatcgggc gagccccttc gcgcaccagc
    35941 ttcatgccgt tataatccat cggattatgg ctggcggtga cttcaatgcc gccatccacg
    36001 ccgagatgga acgtggcgaa atagatctct tcggtgccgg acataccgat atccagcacg
    36061 tcaacgcccg catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga
    36121 cggacatcac cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca
    36181 atgcgccagg cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct
    36241 ttaaagcagg ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc
    36301 gaaacgacca attttgtttt tgtcgttatt tacatctcgg tttcgcattc attgcctgat
    36361 gcgacgctgc cgcgtcttat caggactaca aacccgaacc gtaggtcgga taaggcgttc
    36421 acgccgcatc cgacaaacag cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg
    36481 aagcgcacca catcatcctc ttcgagataa gagccggagc gcacttcaat taaatcaagc
    36541 ggaattttcc ccggattttc caggcagtgc gtcgccccca gcggaatata aatggactcg
    36601 ttttcaccaa gcagtttgat atcaccatca atggtgactt ttgccgttcc cgcgaccact
    36661 acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg
    36721 gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag agtcatattt gccccacgga
    36781 cgatacactt cgcgatgcac ccgatgctca tggcgaccat cggctttgat ctgctcgacc
    36841 acttttttca catcctgtac cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc
    36901 accactacca gatctttcac cccgacggtg gtgaccaggc cagattcggc atacacatag
    36961 ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg
    37021 gcgctgatct cccataatga agaccaggaa ccaacatcgc tccagcccgc atccatcggc
    37081 accacaacag catctgccgt acgttccatg accgcgtaat ccaccgactc ttccgggcag
    37141 gcgagaaacg cttcttcatc cacacgaata aaatcaagat ccggatcgac ggagctcatc
    37201 gctttttcac aggcatcgag aatatccggg cgatattttt tcagttcttc gagatagcgt
    37261 ccggcgcgga acaggaacat gccgctgttc cagtaatatt cgccgcttgc cacataggcc
    37321 tgggcggttt ccagattcgg tttttcgaca aactgcgcca cttcaaaggc caccgtatcc
    37381 tgctcacccg cagacacttc accgcgacga atatagccat aaccggtttc tggtagatcc
    37441 ggcacaatgc caaaggtcac cagcttgccc gcttcggcat atggcatggc attacgcacg
    37501 gcggcacgga acgcgtcttc atcggcaatt acatgatccg ccgccagcac cagcattaac
    37561 gggtcgttct ccgggctatg acgttttgcc gccagcgccg ccagcgcaat ggccggtgca
    37621 gtgttacgcc ctgcaggttc gagaataatg ttctcggtga gtttgttcag ttgacgcagc
    37681 tgttccgcga caataaagcg gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc
    37741 acgccgttca ggcggcagat ggtggtttgc agcatggtga gatcgccttt caggcataaa
    37801 aactgcttgg ggtaaagtac gcgggaaagc ggccataagc ggctaccgga gccacctgcc
    37861 atcacaactg gatagagttt cgactgcgcc ataattatcc ccgaatatca tttataaatt
    37921 gacgtaacac gttctcttta tcgagcgtgc gttcggcata ttcacgtgcc accgtgttgt
    37981 gtttgggcag caggagcgcc tgactaatcc ccgccaccag cgcctcgacc gattccggct
    38041 ctacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg ccccagttct gtgtgggctt
    38101 ctgcggtaat caccgcgtta ccgcctaccg cctaccgcca gaatattggt cagtttcgac
    38161 ggcaatacgg catctgccgc gccgcatttt tgcaccacca gatggcaatc gcccatcttc
    38221 agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt gcgcagtcca
    38281 cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac aatggcaaaa
    38341 atcagcggtt catcgcgcag gcgatcggca gcttcaataa cgttttccag tccctgcttt
    38401 tcaccaatat tgccggagta aagaatgatt tttttgttat ccggtaggcc aagctggtta
    38461 cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcgatttc cgaccagttg
    38521 gggaagaaaa tgatgttatc cgccgccacg cctttttcga tggctttatt catcatcgaa
    38581 cgcgaaatcg tggagacgtt atcgacgtta tgcagtccgc tacgttcgaa cgccgttgcc
    38641 agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag catggcgtcc
    38701 acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagtttcgc cagcaggcgc
    38761 attcccggcg tgcaaaacag cgttggcacc acgccgataa tgcgatccgg cttccagcga
    38821 cgttgcgcca tcaacgggaa aaaactgctg acggcaaaac cgccgagatg caacaggcgt
    38881 ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt ggcggccccc
    38941 tcttctcgtt tgtagcgcta ggcggaatag ttctcgccca cctgccactg cgggtagtaa
    39001 ggcggtgcgg taataacccg cacctcatga ccttgcgccg ccagccattc caccatttct
    39061 ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt taatgccgta gaccagtatt
    39121 ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg ctgttagcgt
    39181 gaacattgtc actggcgagc agtgcgtccg gcgtcagcca gcggtaatcg tcatgctgct
    39241 catccggcag taacagttct tcttccgcta ctctgaagcg aaaaccgagc accacatagt
    39301 gagtggtgaa atccgtgcca gagaagttat cgtcataaaa gtgctgccag acaccgtaaa
    39361 actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc cgctcaaatg
    39421 cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag taaccctgcg
    39481 ccgggcggtt ggttcttttg ccaggcagaa actcgccgcg actgttctcg acaataaagt
    39541 cgagagagac aagcggtgtg gagcgcacta ccgtggcaaa gtcttcctga cgtaaaaaca
    39601 tcattacccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc tggcaagccc
    39661 cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca catcaagcag
    39721 tttgcgcggc gtaccatccg gtttactggc atcaaaaacc acccgacctt tgtaacccac
    39781 cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg tgccgacgtt
    39841 aatgtgcgac agcatcggct gggtgttttc cagccagact tcgtgcgcca gctccatcac
    39901 atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg gtgtaccgct
    39961 gccccacacc accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc gacgcagcaa
    40021 tgctgggatc acatgcgaat tactcgggtg gaagttgtca tgcgggccat acaggttggt
    40081 cggcatgacc gagcggtaat ctcgtccgta ctggcgattg taagattcgc acagtttgat
    40141 cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc tctgcaataa
    40201 ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg atccgagaaa
    40261 cagcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct caatcatcat
    40321 gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc ccactttcgc
    40381 cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca cggcgcggct
    40441 gtccagcagg ttcagctcgt cgcgggtacg taataccagc tccacatcac cgcgctgttc
    40501 gagctgccgc ttgatggcag aaccgaccat cccacgatgg ccagcgataa aaatgcgttg
    40561 tttactcatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga tttcagcaga
    40621 gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat ctctctgagg
    40681 gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgtc gagcagcgtt
    40741 tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc acccggttta
    40801 acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc aacacccgtg
    40861 ccttcaaagc gcagtttgat gcccagctgt gccgctgcca tttccacgaa ctgacgtacg
    40921 gagtactgaa cgccggtcac gataacgaaa tcttccggct gttcctgttg cagcatcatc
    40981 cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc catattgccg
    41041 aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt gattttgcgg
    41101 gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaatacc gttacaagcg
    41161 tacatgccgt aggattcgca gtagttaacg gtgatccagt aggcgtacag tttggcgacc
    41221 gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc ctgcaccaga
    41281 ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag accgaggaag
    41341 cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt atattctggt
    41401 gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc atccggctgt
    41461 acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata atgcagatgg
    41521 aatctcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc ggtgttgaat
    41581 gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag aaactctgcc
    41641 aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga catgtcttat
    41701 tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca ctgcgggatt
    41761 cccccggcaa accacattta ccggaagcga tttaaaaaca ctgcttcgtg cacccacgac
    41821 ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca gccagcattt
    41881 ctcgccaatc
//
LOCUS       DAJTYD010000012        40194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTYD010000012 DAJTYD010000000
VERSION     DAJTYD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.012"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..255
                     /locus_tag="OO585_000606"
     CDS             <1..255
                     /locus_tag="OO585_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6628291.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO585_000607"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO585_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6628292.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO585_000608"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO585_000608"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6628293.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OO585_000609"
     CDS             complement(2907..3182)
                     /locus_tag="OO585_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6628294.1"
                     /translation="MSRTIFCIFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO585_000610"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO585_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6628295.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OO585_000611"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OO585_000611"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6628296.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OO585_000612"
     CDS             5142..5468
                     /locus_tag="OO585_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6628297.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OO585_000613"
     CDS             5652..6371
                     /locus_tag="OO585_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6628298.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="OO585_000614"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="OO585_000614"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6628299.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="OO585_000615"
     CDS             7710..8717
                     /locus_tag="OO585_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6628300.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO585_000616"
     CDS             complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO585_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6628301.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10001..10594)
                     /locus_tag="OO585_000617"
     CDS             complement(10001..10594)
                     /locus_tag="OO585_000617"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6628302.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVTQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10602..10892)
                     /locus_tag="OO585_000618"
     CDS             complement(10602..10892)
                     /locus_tag="OO585_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6628303.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO585_000619"
     CDS             complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO585_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6628304.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO585_000620"
     CDS             complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO585_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6628305.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12195..13175
                     /locus_tag="OO585_000621"
     CDS             12195..13175
                     /locus_tag="OO585_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6628306.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO585_000622"
     CDS             complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO585_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6628307.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13767..14330)
                     /locus_tag="OO585_000623"
     CDS             complement(13767..14330)
                     /locus_tag="OO585_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6628308.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO585_000624"
     CDS             complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO585_000624"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6628309.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO585_000625"
     CDS             complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO585_000625"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6628310.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO585_000626"
     CDS             complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO585_000626"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6628311.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17015..17512)
                     /locus_tag="OO585_000627"
     CDS             complement(17015..17512)
                     /locus_tag="OO585_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6628312.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO585_000628"
     CDS             complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO585_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6628313.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO585_000629"
     CDS             complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO585_000629"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6628314.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20877..21092)
                     /locus_tag="OO585_000630"
     CDS             complement(20877..21092)
                     /locus_tag="OO585_000630"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628315.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21228..21359
                     /gene="yqgB"
                     /locus_tag="OO585_000631"
     CDS             21228..21359
                     /gene="yqgB"
                     /locus_tag="OO585_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6628316.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21368..23344
                     /gene="speA"
                     /locus_tag="OO585_000632"
     CDS             21368..23344
                     /gene="speA"
                     /locus_tag="OO585_000632"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6628317.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23481..24401
                     /gene="speB"
                     /locus_tag="OO585_000633"
     CDS             23481..24401
                     /gene="speB"
                     /locus_tag="OO585_000633"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6628318.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO585_000634"
                     /gene_synonym="yggG"
     CDS             complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO585_000634"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6628319.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25643..27634
                     /gene="tkt"
                     /locus_tag="OO585_000635"
     CDS             25643..27634
                     /gene="tkt"
                     /locus_tag="OO585_000635"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6628320.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            27948..28391
                     /gene="cmtB"
                     /locus_tag="OO585_000636"
     CDS             27948..28391
                     /gene="cmtB"
                     /locus_tag="OO585_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6628321.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHG"
     gene            28419..29807
                     /gene="cmtA"
                     /locus_tag="OO585_000637"
     CDS             28419..29807
                     /gene="cmtA"
                     /locus_tag="OO585_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6628322.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29822..31099
                     /locus_tag="OO585_000638"
     CDS             29822..31099
                     /locus_tag="OO585_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6628323.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31096..32061
                     /gene="yggF"
                     /locus_tag="OO585_000639"
     CDS             31096..32061
                     /gene="yggF"
                     /locus_tag="OO585_000639"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6628324.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32083..32592
                     /gene="fumE"
                     /locus_tag="OO585_000640"
     CDS             32083..32592
                     /gene="fumE"
                     /locus_tag="OO585_000640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6628325.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32589..33302
                     /locus_tag="OO585_000641"
     CDS             32589..33302
                     /locus_tag="OO585_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6628326.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33274..33951)
                     /locus_tag="OO585_000642"
     CDS             complement(33274..33951)
                     /locus_tag="OO585_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6628327.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33945..34622)
                     /locus_tag="OO585_000643"
     CDS             complement(33945..34622)
                     /locus_tag="OO585_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6628328.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34610..35317)
                     /locus_tag="OO585_000644"
     CDS             complement(34610..35317)
                     /locus_tag="OO585_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6628329.1"
                     /translation="MHPFTSLILWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35318..35896)
                     /locus_tag="OO585_000645"
     CDS             complement(35318..35896)
                     /locus_tag="OO585_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6628330.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35919..36350)
                     /locus_tag="OO585_000646"
     CDS             complement(35919..36350)
                     /locus_tag="OO585_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6628331.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36722..37741
                     /gene="epd"
                     /locus_tag="OO585_000647"
     CDS             36722..37741
                     /gene="epd"
                     /locus_tag="OO585_000647"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6628332.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37791..38954
                     /gene="pgk"
                     /locus_tag="OO585_000648"
     CDS             37791..38954
                     /gene="pgk"
                     /locus_tag="OO585_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6628333.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39169..>40194
                     /gene="fbaA"
                     /locus_tag="OO585_000649"
     CDS             39169..>40194
                     /gene="fbaA"
                     /locus_tag="OO585_000649"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6628334.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSM"
BASE COUNT         9854 a        10080 c        10737 g         9523 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaaa tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt
     8821 tgccacatgt aggctggata aggcgttcac gccgcatccg gcaatacaag tttactcagc
     8881 cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta tctgccagta
     8941 atccgcacat tctgtgagat aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac
     9001 atcttcgcaa agcccggtat acgcactaaa ctctgcgcgt ggcgcggcct ccagcaagcg
     9061 aaagcggttc ataaagaact caaacggctt atctgcggct tcgacatcac gctggctttc
     9121 cagatacctt ccttgcataa aaccacgagg atgacgcgtt ttagtggtac gcagaatgcg
     9181 cccatccggg aaggtgactt tgccgtgcgc gccgcagcca ataccgatgt agtcaccaaa
     9241 tcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag cggaagtttc
     9301 atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa tatcccacag
     9361 cgcgtcatcg tccggcagca ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt
     9421 cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta gatcgccaag
     9481 cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa
     9541 ccctaaaccg ctcgccagct ttgccgctcg tttcgcttct tgcgggccat gaatgcgccc
     9601 aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc ggttcacacc
     9661 agcacgctga taatcgacaa agcgatcggc ttctaccgta ccagggttcg cttccatagt
     9721 aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat
     9781 cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct ttacttcacg
     9841 gccctgagcg taagccacat cattgtccag atcgcaaagc agatgctgaa cataatcgtc
     9901 gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc atttctgcac
     9961 gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta cgtaaagcgt
    10021 ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct tcgcgggtca
    10081 gttcggcggc ggttttccct tcggaaggta cgaagaagat tggatcataa ccaaagccac
    10141 cagtgcccgc tggttcgcga gtaatcacgc ccggccagct gccgtggcat accagcggag
    10201 tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgtgcc tgacgttggt
    10261 cgtccggtac gtctttcagt gtttccaaca gtttttgcag attcttttga tcggtcgcgt
    10321 cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca tctaccgcca
    10381 gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg cgcgctttca
    10441 gaatcgcgtt ttcgataaag gttaggccgg tttcttcagc ggaatcaacg ccgaggtctg
    10501 tttgtgtcac gatatcaaga ccaaagtcgc taagcagcga cgccagctca cgcactttac
    10561 cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat taacgccgcg
    10621 atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg gccaagttcg
    10681 cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag aaacttcacc
    10741 agatgactgt tggcctggcc gtcaactggc ggcgcggtaa tggcgacttt aacttcgtcg
    10801 ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag ccgtaaaacc
    10861 agaccgtcat cattaactgt tacggcattc ataacgccat ccacatcccc ggcagcagga
    10921 tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac agcagcagaa
    10981 cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg cgaatcgggc
    11041 gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga ccctggctta
    11101 cccagctcat aatcgccatc accagcagga cccagaaaat cagcaagccg atggttttca
    11161 gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact ttaaacagca
    11221 caatggcttt gataaaactg agaatatagg caaccagcag cgaggcgctg tcaatgggac
    11281 ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgcgttacc ttcactacaa
    11341 actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg cgtaataaca
    11401 gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt
    11461 tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag tgccgatacg
    11521 aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc ccagagagag
    11581 cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca tttggcgtgc
    11641 aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca gcccgcgcag
    11701 acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg ccagttgaat
    11761 cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa gagggggaag
    11821 ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca
    11881 ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc caataaaatg
    11941 ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta ccccttcctg
    12001 aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc
    12061 aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt
    12121 tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt
    12181 ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct
    12241 cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg ctaatggaag
    12301 ctgcgccgtt tgatgcgctg gacgtcgaag agctactgcg ggagtggctg gatgacgatc
    12361 agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc
    12421 agcgattgcg tggcagcgca ttcgcgcaac ggcatggcat ttcgctggcg ttacggctgt
    12481 taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc aacggtattg ccggaattac
    12541 tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca
    12601 cgctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt ctgacgctgg
    12661 aagatcctgt tgaatatatc tatgccagcc agcgatgttt gatccagcag cgggaaattg
    12721 gtttgcactg tatgacgttc gcatcgggat tgcgggccgc attgcgggaa gatcctgatg
    12781 tgattttgct cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag
    12841 aaaccgggca tttggtgcta gcaacattac atacgcgtgg tgccgcgcag gcagttgagc
    12901 gactggtgga ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta
    12961 gtttacgggc cgtgttgtca caaaagctag aagtggataa acaggaagga cgcgtggcgc
    13021 tgtttgaatt attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc
    13081 accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga
    13141 gttatcagca gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt
    13201 tatagggcct ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcagaccta
    13261 cagccgctgc cagatgtgtg ctggataagg cgttcacgcc gcatccggca agatggcggt
    13321 aacgactatc cgccaggctt ttagatcgcc ttaatatccc tgctcgaaat agctttcgag
    13381 aataataacc gcagaggcag agtcgacttt gcctttgttg agcgcccgat agccgccctg
    13441 ttcaaacaga ccggaacggg cttccacggt gctaagacgt tcgtcatgga gctttacttc
    13501 aacaccgaaa cgaccatgaa tacggttggc aaatttacgc gctctggcag tcaatggttg
    13561 ctcggtgccg tccatattca gcggcaaacc gacgatgatt tcgtccggct gccactcttt
    13621 cagtaaacgt tcgatgaggt tccagtccgg cgtaccgtcc tgtgctttaa ttgcaggcaa
    13681 agggcgagca gtgccggtaa tgcgttggcc gaccgctacg ccaatgcttt tggtgccgaa
    13741 gtcgaaggcg agtaaggttc cactcatcag gcgtgccctg ccacaccagg catggtgaga
    13801 atatccacac caatcagttt tgccgcctcg cgccagcggt cggcaatcgg cgttttgaac
    13861 agaatattca gatccgccga ggccgttagc cacgcgttat cgagaatttc ttgttccagt
    13921 tgaccttttt cccaggaggc atagcccaga gccaccaata cgtcagacgg ttgtttatcg
    13981 gtgccgagcg tttccagcac atcgcgggaa gtggtcatca ccgtgttgtc tgaaatgcga
    14041 atgctggaag caaaattgga gggcggagta tgcaaaataa acccgcgatc ttcagccagt
    14101 gggccgccga gcataacagg tttatccaga cggattgatt catcacgcgg ctccggcgtg
    14161 atcttcagct tttccagaat cccttcaatt ttgagatttt ccagcggctt gttgacgatg
    14221 atccccattg caccattggt attatgttcg caaatgtaga ccacggaacg acggaaaatc
    14281 ggatcctgga gagcaggcat ggcaataaga aagtgatgct gtaaattcat tgtcagaggt
    14341 tctgtttcct ggttcaaaaa agcaacagcg cccagtatgc gtggaaagaa gggcgctgtc
    14401 actcagagtc tcaacgagat ccttctcgct aaggtgggtt actgctgctg taaacgtgct
    14461 tcgatggcat ccattaacat tccggtgatc gacaccggaa actctgcttc aatctcacga
    14521 atacaggttg ggctggtgac gttaatttca gtcagacggt cgccgatgat atccagacca
    14581 acaaaaatca gccctttttc tttcagcgtc ggcccgatct gacgggcgat tttccagtca
    14641 ctttccgtca gcggccgagg ttcaccgcga ccaccggcag ccagattgcc acgggtttcg
    14701 cccccctgcg gaatacgcgc caggcagtac ggtaccggct cgccatccac caccagcacg
    14761 cgtttgtcgc catctttaat ggctggcagg taattttgcg ccatgcagta gcgagtgcca
    14821 tgctcagtca gggtttcggc aatcacgccg aggtttggat cgccttcttt cacgcggaaa
    14881 atcgacgcgc cgcccatacc gtccagcggc ttaagaatga tgtcgctgtg tttctcccag
    14941 aacgctttta gctgcgcttt attgcgcgta accagcgttt ctggcgttaa gtcagagaac
    15001 caggcggtaa acagtttctc gttacagtcg cgcaggctct gcggcttgtt aacgatcagc
    15061 gtccctttct cttcggcacg ttccagaata taggtcgcgt agataaactc ggtatcaaac
    15121 ggcgggtctt tacgcatcag gagcacatcg agatcggcca gcggcagatc ctgttcaccg
    15181 acgaacgaaa accactcttc gtagttctgc ttcacgttca gcgtgcgggt atgggcgcga
    15241 gcttcgccat tgatcagata cagatcgccc atctccatat agtgaagttc gtaaccacga
    15301 cgctgtgctt ccagcaacat agcgaaactg gaatctttct tgatgttgat gtttgcgatg
    15361 gggtccatca cgatgccgag cttgatcatt attcttctcc gttagcccaa atcgccaaat
    15421 cgtacttgta gcgcggtaat ggcggtgagc gcagttgtct ctgtacgcaa aacgcgaggt
    15481 cccaacagga tatcagtaaa ttgatagcgg gcagtcatgg caatttcatc tgccgataaa
    15541 ccgccttccg ggccaatcag caggcggacg cgttcaaccg gtaacggcaa cgtattgatg
    15601 ctgttactgg cgcgcgggtg aagattcagt ttcagtcctt catcctgctc tgcacaccag
    15661 gcttccagat ccatcgctgg acggatttcc ggcacccggt tacgaccaca ctgctcacag
    15721 gcagcaattg caatcttctg ccactgctgg agcttcttgt tcagacgttc actatccagt
    15781 ttaacgccgc agcgctcaga aaaaagtggc gtaatgaggc ttacaccgag ttcgatcgat
    15841 ttctggatcg taaattccat tttttcacca cgcgacatca cctgaccgag gtgaatatgc
    15901 agcggagatt cgcgatcgtc gatcttgcct tccagcacct tcacttccac gctttttttg
    15961 ctggcgctgg taatttcggc gtcaaagacc tggttgctac cgtcaaacaa ttgcaacgcc
    16021 tgccccggcc ccatgcgcag tacgcgcccg atatggttgg cggcatcttc gcaaagcgcg
    16081 atgtgagaat ggctggtcag tggttcagga tgataaatgc gggggatacg catagttaaa
    16141 aatccgcgtc gtctccccac gcggttgtac gcgtggggta ggggttaaca aaaagagtcc
    16201 cgctagtgta ggttagctct ttcgcgcctg gcaagcgcgt tgcacatacg ggttatgatt
    16261 gccttgcacc ttcgcgatgc gttcatcgcg ctcgcactcc cagtcggtaa ccggatacat
    16321 cttgttccat gcgttgaaca gctgcgtttg ctggcgagag agtgtcaggt tgtattggtc
    16381 gcgcatatag aagtaggtgc gcgcaatggc accgcgtgca cgcgctggtg gttcggcagc
    16441 tttttctttg aaatcgacct tcatggcgca ttgaccgtac tggccttcgc cgccattcca
    16501 ctggctgtac ataaagttgc cgcgatcgcc attcacctca ccgactgacg gctgcaggtt
    16561 atgcatatcg ctttccatct tgcgatagac cggatcttta gcgcagtttt tacgtccacc
    16621 gtcctgccag cactggcgct ggtgaccgaa ctgccaggcg ggaacgacgt gttcccactc
    16681 tacgcggctg gctcggtttt catttttgcg cacctgatag ccgcacgatt gcagatcaac
    16741 aacgcctttt ttgccctgcc agttaatttt acatccgcaa taaaacgtac cgggcgcgtc
    16801 agcgtggact tttaccgccg cggctttcgc ctgagaaaaa ctattgatac cttcggccaa
    16861 cgccgggccg gaaaatgctg cgctcagtac caccgcagca atagacaaat aacggtacat
    16921 cacttactcc gtgttaaaac gagccagcaa cgtagcgaaa gttgttttgg tatgcaatca
    16981 gccagatcag gaaagttcct gatagttcag atggttattt tgcgaccagt tgttcgccgc
    17041 agtgaacaca gcgatagacg gcttcgccac gaactacgcg attatggcgg cgtacggtaa
    17101 gctgatgctc ctggcacttg cagcggtagg ggaaggtgtt gcgacgcacg gattgcagtt
    17161 cgaactgatg cgtacgacgg gcgggaacac ccagcacgtt ttccatcatc cacttccact
    17221 ctttgccatg tggtgctacg cgcccgaaat gtttccatac cagcaaatgt gccagttcgt
    17281 gcggcaccac ttcttcaata aaggcttcac tgttttccaa cagcaaaacg ggattgaggc
    17341 ggatttcata gctttccagc caggctgttc cggcggaggt gccgcgctgg gtataagaga
    17401 gttttggctc cgggtagtta cgccccaatt tcaggttagc ctgggcgagt ttttcccgca
    17461 ggcgacgcat aacggcctgt tggatggcga tagggagacg ggatgttttc atagcggcag
    17521 aggatagagc gaagaaaaga tgactgcaag aggtggcttc ctccgcgatg ggaggaagct
    17581 tggggagatt aatcgtgagc gcctatttcg cgcagtttac gacctttcat cagattacgt
    17641 tcaatatgtt ccagcgaaac gtgtttggtt tccggtacca gccacaatgt cagcaggata
    17701 aacagtacgt tcagagccgc atacacccag aaggtgttgg cgttacccag tgtgttgagc
    17761 atggtcagga acgttgcgcc aacgatcatg ttggcaatcc agttggtggc ggtagagcag
    17821 gtgatgccaa aatcgcggcc tttcagcggc tgaatttcgg agcacagtac ccaaatcagc
    17881 ggaccggcac tcatggcaaa accgacaata aacatcagca gcatggcgat ggcgaaatac
    17941 tgcgctgacg gagagtgaat accgatatgc atcattgtac cgagtacgcc catgccagca
    18001 gccatcacca ggaagcccag cgttagcgtt ggtttacgtc cccagcggtc aacaaggccg
    18061 attgcgataa aggtggcaag tacgttggtc aggccgacaa tcactgtccc ccacatttgc
    18121 tcggtagtgt tggtataacc cgccagttcg aagattttcg gcgcgtaata catgatgacg
    18181 ttcatcccgg tgaattgctg cattacctgt aacagtacgc caaggaacac cgcgcggcgg
    18241 aagttgctat tctctttaaa cagcgcccag ccactctgtt taacctgcaa actttcacgg
    18301 atttcatcca gttcgcgttt cgcttccgcg ctggtgtcac gcaggcgtag cagcacgcgt
    18361 tcggcatcaa caaaacggcg tttggcggca aaccaacgtg ggctgtctgg caggaagaag
    18421 acaccaatca gcagcaaaat tgccgggatg ataatcacac ccagcatcca gcgccatgca
    18481 ccggtgtagc tgaaggcggt atcagaaaga taagcaccga ggatcccgat agtgatcatc
    18541 aactgataca tcgagatcat actgccacga attttttccg gcgcaatttc agagaggtac
    18601 agcggtgcgg tataagaggc cacacccacc gccagcccca gtagaacgcg ggaaagaatc
    18661 agtacttcaa cgtttggcgc agccgcagag aacagcgaac cggcaacaaa caaaattgcg
    18721 ccgatcatca ggctcttttt gcgcccgagt ttaaaggaga gccagccgct gcccaccgca
    18781 ccgactgccg caccgaacat catggagctt acgacccatt cttgcgtgtg cgaagtaatc
    18841 tggaattcat ctgcaataaa cggcagtgcg ccagcaatta caccgatatc caggccaaag
    18901 agtaatcccg ccagagcggc aaggaagcag acgaaaaacg tcattgcctt gtttgaccgc
    18961 ccctgttttt tagcgtcagg catgatgccc tccaatatgg ttatttttta ttgtgaatta
    19021 agataggtga gtacgacgta aaaagatgtg aagcaaatca catcagtgta atcgcttaca
    19081 ctaacattta tcttagttat ggtttaagtg catgaaaata attgagttat atcatcatag
    19141 attaaacgct gttatctgca attaagactt tactgaaaag aaatgtaaca actgtgaaaa
    19201 ctgaataata atatgaaaat attacaaata tttggctggt gttattggtg taatcggttt
    19261 cagtagtgat gataatttgc gataaaacgt gttgaggaag aatgatgtta ttgtgcgggc
    19321 agctttttgc cggatgcggc gtgaatgcct atccaccgcg gtgaggtaaa agaaaaaggc
    19381 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    19441 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    19501 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    19561 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    19621 gtaccgaaag tttctaccat gatggaggtc ggttcagcca cgccgattgc gtaggaaacc
    19681 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    19741 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc tccgccaccg
    19801 tgacgtgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    19861 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaatgt ggtggcagaa
    19921 gtcagccatt cagcgggcag aattggcttg atgatctctt ccattaccgc ttcttgcagc
    19981 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc aataccaacg
    20041 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    20101 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    20161 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    20221 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    20281 ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    20341 tagccaattt cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg
    20401 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    20461 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    20521 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    20581 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    20641 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    20701 attaacatct ggatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    20761 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttt ctgacaatgc cggcattctg
    20821 cgtatttata ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    20881 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    20941 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgta
    21001 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    21061 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    21121 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    21181 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    21241 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    21301 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    21361 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    21421 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    21481 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    21541 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    21601 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    21661 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgagg gaatcctacg gctataacgg
    21721 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgccgcgtga ttgagtccct
    21781 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    21841 gctggcacat gctggcatga cccgtagcgt catcgtctgc aacggttata aagaccgcga
    21901 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    21961 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    22021 tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt aaatggcagt cttccggcgg
    22081 ggaaaaatcg aagttcggcc tggctgcgac tcaggtactg caactggttg aaacgctgcg
    22141 tgaagctggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    22201 gaatattcgc gatatcgcca cgggcgttcg tgaatccgcg cgtttctatg tggaactgca
    22261 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggactatga
    22321 aggcacccgt tcgcagtctg actgctccgt aaactacggc ctcaatgaat atgccaacaa
    22381 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    22441 caccgaatcg ggtcgtgcgg tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    22501 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    22561 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    22621 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    22681 cacctttagc ctgcaagaac gtgcatgggc agaacaactc tatctgagca tgtgccatga
    22741 agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt ccgataatcg acgaactgca
    22801 ggaacgtatg gcggacaaaa tgtacgtcaa tttctcgctg ttccagtcaa tgccggacgc
    22861 atgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    22921 acgtcgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    22981 tgatggtgat ggtattgcca cgacaatgcc aatgccggag tacgatccag agaatccgcc
    23041 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    23101 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    23161 actgtctgac gaaggcgata ccgttgcgga tatgctgcaa tatgtgcagc tcgatccgaa
    23221 aacgctgtta actcagttcc gcgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    23281 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacttatc tggaagatga
    23341 gtaagtccgt attacttgaa tccgcttaat ttagcggtga taatccgcca caatttattg
    23401 tgacaaatcc aacccttcct cgtcgggcct aacgacgcgg aagggttttt ttatatcgac
    23461 tttgtaatag gagtccatcc atgagcacct taggtcatca atacgataac tcactggttt
    23521 ccaacgcctt tggtttttta cgcctgccga tgaacttcca gccgtatgac agcgatgctg
    23581 actgggtgat tactggtgtg ccgttcgata tggccacttc tggtcgtgcg gggggacgcc
    23641 acggtccggc agcgatccgt caggtttcga cgaatcttgc ctgggaacac aatcgcttcc
    23701 cgtggaattt cgacatgcgt gagcgcctga acgtcgtgga ctgcggcgat ctggtatatg
    23761 cctttggcga tgcccgtgag atgagcgaaa aactgcaggc acacgccgag aagctgctgg
    23821 ctgccggtaa gcgtatgctc tctttcggtg gtgaccactt tgttacgctg ccgctgctgc
    23881 gtgctcatgc gaagcatttc ggcaaaatgg cgctggtaca ctttgacgcc cacaccgata
    23941 cctacgcgaa cggttgtgaa tttgaccacg gcaccatgtt ctataccgcg ccgaaagaag
    24001 gtctgatcga cccgaatcat tccgtgcaga ttggtattcg taccgagttt gataaagaca
    24061 acggttttac cgtgctggac gcctgccagg tgaacgatcg cagcgtggat gacgttatcg
    24121 cccaagtgaa acagattgtg ggtgatatgc cggtttacct gacctttgat atcgactgcc
    24181 tagatcctgc tttcgcgccg ggaaccggta cgccagtgat tggcggcctg acctccgatc
    24241 gcgccattaa actggtacgc ggcctgaaag atctcaacat tgttgggatg gacgtagtgg
    24301 aagtggctcc ggcatacgat cagtcggaaa tcaccgctct ggctgcggcg acgctggcgc
    24361 tggagatgct gtatattcag gcggcgaaaa agggcgagta agcaccagat gcgatgcgca
    24421 cgggtaaaac gtgccattaa tgtcggatgc ggcgtgaacg ccttatccga cctacgttcg
    24481 gcacccgtag gccggataag atgcgccagc atcgcatccg gcaatgcgca caaggtaaca
    24541 aatgtgccat taatgtcaga tgcggcgtga acgccttatc tgacctacgt tcgacaccac
    24601 caggctttac ttaatcccat ccgcgctcat gcgatcgcga atatgctggg cgcgttcggc
    24661 ggatgccgga tggtcgtcaa acattgagct ttggcggcct tcttccagtt tcgccagttt
    24721 ttcaaagctg gtggcaagac ctgccgggct gatgccgcgt tggcgcagaa gatcgtaaga
    24781 ataatcatcg gcttctgctt cctggcgctg ggagaattgc gaattgacta atttctcgcc
    24841 cagatcacca agttgtgatt gagataaact gccgacaatc ccgcccgcag aggccgcagc
    24901 tactcgcacg gcatttgtac caagcgccac ctgcatccct ttcttcacat ggcctaacgc
    24961 cacgtgcccc atctcgtgac cgatcaccgc ttcgacttcg ttatccgtca tcatatccat
    25021 cagcccgcta tagacgcgga tacagccgtt agccattgca aaggcgttca catccttcgc
    25081 catatacact ttgtaattca ccggctgacc gttgatattg ttgcctagcg cattggcaat
    25141 agttgtcaga cgtttagcgt attcgctatt ggctggcgca atcgtcgcct tgctgtccat
    25201 ctcctgacat gcctgatcgc tcagggtttt cacctgcgca tcactcaaac tgtaagcctg
    25261 aaaagcttcc gctcctgatg agagcagtcc gttggagtcc atattctggc aaccagtcag
    25321 taccgttgcc acgctcattg ctaccaataa ggcgcgaatt ttcatgtttt ctcttccgca
    25381 caaggtcagg attattctaa aaatgcgccg tttgcaggtg aatcgacgct cagtctcagt
    25441 ataaggaaaa gcgcagcgga cgggcgagta gattgcgcaa catgcgagca tgatccagag
    25501 atttctgaag cggcaaaagg atgttccatg tacatgacgc gcggcttgcg gtaaattgtt
    25561 ggcaaatttt ccggcgtagc ccaaaacgcg ctgtcgtcaa gtcgttaagg gcgtgccctt
    25621 catcatccga tctggagtca aaatgtcctc acgtaaagag cttgccaatg ctattcgtgc
    25681 gctgagcatg gacgcagtac agaaagccaa atccggtcac ccgggtgccc ctatgggtat
    25741 ggctgacatt gccgaagtcc tgtggcgtga tttcctgaaa cacaacccgc agaatccgtc
    25801 ctgggctgac cgtgaccgct tcgtgctgtc caacggccac ggctccatgc tgatctacag
    25861 cctgctgcac ctcaccggtt acgatctgcc gatggaagaa ctgaaaaact tccgtcagct
    25921 gcactctaaa actccgggcc acccggaagt gggttacacc gctggtgtgg aaaccaccac
    25981 cggtccgctg ggtcagggta ttgccaacgc agtcggtatg gcgattgcag aaaaaacgct
    26041 ggcggcgcag tttaaccgtc cgggccacga cattgtcgac cactacacct acgccttcat
    26101 gggcgacggc tgcatgatgg aaggcatctc ccacgaagtt tgctctctgg cgggtacgct
    26161 gaagctgggt aaactgattg cgttctacga tgacaacggt atctctattg atggtcacgt
    26221 tgaaggctgg ttcaccgacg acaccgcaat gcgtttcgaa gcttacggct ggcacgttat
    26281 tcgcgacatc gacggtcatg acgcggcatc catcaaacgc gcagtagaag aagcgcgcgc
    26341 agtgactgac aaaccgtccc tgctgatgtg caaaaccatc atcggtttcg gttccccgaa
    26401 caaagccggt actcacgact cccacggtgc gccgctgggc gacgctgaaa ttgccctgac
    26461 ccgcgaacag ctgggctgga aatatgcacc gttcgaaatc ccgtctgaaa tctatgctca
    26521 gtgggatgcg aaagaagcag gccaggcgaa agaatccgca tggaacgaga aattcgctgc
    26581 ttacgcgaaa gcttatccgc aggaagccgc tgaatttacc cgccgtatga aaggcgaaat
    26641 gccgtctgac ttcgacgcga aagcaaaaga gttcattgct aaactgcagg ctaacccggc
    26701 gaaaatcgcc agccgtaaag cgtctcagaa tgctatcgaa gctttcggtc cgctgttgcc
    26761 ggaattcctc ggcggttctg ctgacctggc accgtctaac ctgaccctgt ggtctggttc
    26821 taaagcaatc aacgaagatg ctgcgggtaa ctacatccac tacggtgttc gcgagttcgg
    26881 tatgaccgcg attgctaacg gtatctccct gcacggtggt ttcctgccgt acacctccac
    26941 cttcctgatg ttcgtggaat acgcacgtaa cgccgtacgt atggctgcgc tgatgaaaca
    27001 gcgtcaggtg atggtttaca cccacgactc catcggtctg ggcgaagatg gcccgactca
    27061 ccagccggta gagcaggtcg cttctctgcg cgtaaccccg aacatgtcta catggcgtcc
    27121 gtgtgaccag gttgaatccg cggtcgcgtg gaaatacggt gttgagcgtc aggacggccc
    27181 gaccgcactg atcctctccc gtcagaacct ggcgcagcag gaacgaactg aagagcaact
    27241 ggcaaacatc gcgcgcggtg gttatgtgct gaaagactgc gccggtcagc cggaactgat
    27301 tttcatcgct accggttcag aagttgaact ggctgttgct gcctacgaaa aactgactgc
    27361 cgaaggcgtg aaagcgcgcg tggtgtccat gccgtctacc gacgcatttg acaagcagga
    27421 tgctgcttac cgtgaatccg tactgccgaa agcggttact gcacgcgttg cggtagaagc
    27481 gggtattgct gactactggt acaagtatgt tggcctgaat ggcgctatca tcggtatgac
    27541 caccttcggt gagtcagctc cggcagagtt gctattcgaa gagttcggct tcaccgtcga
    27601 caacgtcgtt gcgaaagcaa aagaactgct gtaattagca tttcgggtaa aaaggtcgct
    27661 tcggcgacct tttttattac cttgatatgt ccgtttgcgg acaagcaata gataaagcgt
    27721 gttgcagatc acaaatattt atatgcaata aatatcaatt atgtaatatg catcacgata
    27781 tgcgtattga catttgttgt tatatctata actcaatgtt atataagaaa ttaataattc
    27841 actgttttca aaacaccggt ttccctgctc aattgctttc attaaaccgc acgagtattt
    27901 tatttttacc gccggacggt accctaaaaa taaaataagg ataatttatg cggcttagtg
    27961 actattttcc agaatcatca atctcagtta tacattcagc aaaagattgg caggaagcta
    28021 tcgatttctc gatggtatca ttgctggata aaaactatat cagcgagaat tacattcagg
    28081 caattaaaga ttccaccatt aacaatggcc cttattatat tctcgcacca ggcgtggcaa
    28141 tgcctcatgc gcgaccggaa tgtggggcgc ttaaaaccgg gatgtctttg acattacttg
    28201 aacaaggtgt ttattttccg gggaatgacg aaccaattaa attactcatc ggactctctg
    28261 ctgccgatgc cgattcgcac attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag
    28321 aagaaatact cgaacaactc ttaacagcat catcagaaaa acaattagcg gacattatca
    28381 gccacggata attactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg
    28441 caaaggtcca ggcttttggg ggctttttga ctgcaatggt catccccaat attggtgctt
    28501 ttattgcctg gggttttatt actgcgttat ttattcccac cggttggctg cctaacgaac
    28561 atttcgccaa aattgtcggc ccgatgatta cctatttatt gcccgtgatg attggttcta
    28621 caggtggtca tctggtcggc ggtaaacgcg gggcggtcat gggcggaata ggtactattg
    28681 gtgtgatcgt tggcgcagag atcccgatgt tccttggctc aatgatcatg gggccgctcg
    28741 gtgggttggt cataaaatat gtcgataagg cactggaaaa acgcatacct gccggttttg
    28801 agatggttat caataacttc tcattaggta tcgcggggat gctcctttgt ctgctgggtt
    28861 ttgaagtgat cggcccggcg gtgttaattg ccaatacttt cgtcaaagag tgtatcgagg
    28921 cgctggtaca tgcgggttat ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc
    28981 ttttccttaa taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt
    29041 cggttaacgg taaatccatc ttctttatgg tggcctctaa cccaggtccg ggcctggggc
    29101 tgctgctggc gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga
    29161 tgattattca cttcctcggt gggatccacg aactgtattt cccatatgtg ctgatgaagc
    29221 cgctgactat tattgccatg attgcgggcg gtatgtctgg tacctggatg tttaacttac
    29281 tggacggtgg tctggtggct ggcccaagtc tggggtctat ctttgcttac ctggcactga
    29341 cgccgaaagg ctcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct
    29401 ttgctattac ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg
    29461 agtttgctca gtcagccaat gcggttaagg cgatgaaaca agagggtgca ttctcgttaa
    29521 gcagggttaa gcgtatcgcc tttgtttgcg atacggggat gggctccagt gcgatgggcg
    29581 cgaccacctt ccgtaaacgc ctggaaaaag cggggctggc aatagaagta aaacattacg
    29641 cgatagaaaa cgtgcctgcg gatgccgata tcgtcgtcac tcacgccagt ctggaagggc
    29701 gcgtgaaacg tgtgacggat aaaccgctga tattgattaa taactatatt ggcgatccaa
    29761 aactcgacac tttatttaat caattaaccg ccgaacataa acactgatta cagaggtaaa
    29821 aatgaaaacc aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga
    29881 actgccagaa attaccgata atgaactgtt agtgagtgta atttctgaca gcgtctgttt
    29941 atcgacctgg aaagcggcgt tactcggtag tgaacataaa cgcgtacccg acgatttaga
    30001 aaatcatccg gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa
    30061 tctcactggc aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc
    30121 aagcggatat tcagcaggct acagctacga atattttggc ggtaacgcca cctatatgat
    30181 tattcccgaa atcgccatta atttgggctg cgtattaccg tatcacggct cttattttgc
    30241 tgcggcgtcg ctggtagagc ctatgtgctg cattattggt gcttatcatg ccaattatca
    30301 caccacgcaa tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact
    30361 gctggcgtgt gcgggtccga tgggcattgg cgctatcgat tacgccatta acggtggcat
    30421 acaaccgtca cgggtggtgg tggtcgatat cgacgacaaa cgcctggcgc aggtgcagaa
    30481 gctgctgccg gtggatctgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa
    30541 agggatgagc gatccggtcc agacgctgcg ggcgctgaca ggagatgtcg ggttcgatga
    30601 catttttgtt tatgcggcgg tgcctgctgt cgttgagatg gctgacgaat tactggcgga
    30661 agatggctgt ctgaacttct ttgccgggcc gacggataaa aacttcaaag tgccgtttaa
    30721 tttctacaac gtccattaca acagcacgca cgtagtcggt acatccggcg gttcaacgga
    30781 cgacatgaaa gaggcgattg cgttaagcgc cacggggcag ttgcagccgt cctttatggt
    30841 cacgcatatc ggtgggctgg atgcggtgcc agataccgtg ctcaatctgc cggatatccc
    30901 tggcggtaaa aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt
    30961 tgccgaaaaa ggcaaaaccg atccgctatt taaagagttg gcgcggctgg ttgaggaaac
    31021 gcacggcatc tggaatgaac aggccgagaa atatctgctg gcacaatttg gcattgatat
    31081 cggggaggcc gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca
    31141 gcgttggctg cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg
    31201 gtcaccgcga tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc
    31261 gaaggagaaa ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat
    31321 gggccagaag tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg
    31381 cagagcaatg cgctggcggt catggctttc gcccctcggg atagcctgtt acatgcgccc
    31441 gatatgtata tgaaaaagct ggttgttaat cgactggctg ccggggtgat tgatctgtct
    31501 ttgcctctgg cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag
    31561 ctgcgcatgg ttacgctgga taaaccgcgc ctgagtgccg cgattgagga agcaacacag
    31621 ctgggcgtga aggtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc
    31681 tggcaggata atccttacga tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt
    31741 tctgcctgtg ccgttaaagc attaggcggg gatatgcagg ccgagctgat tgacttttgt
    31801 caggctaaag gggattacac ggaaaatcgg cagattgcag agcaggagag caagcgttgc
    31861 aaggcaatgg gcgttgacgt taaccgtgtg tactcgctcg atgaactggt aagggggaac
    31921 gatatcctct ttagcgctac gggcgtgacg ggcggcgagt tggtgaacgg tatccaacag
    31981 acggcgaatg gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat
    32041 ataatagact ccctgcattg atggtgatct atcggaagac caatggcgaa gctgacagaa
    32101 gatgatgtgc ttgagcaact ggatgcacag gacaatttat tttcatttat gaaaactgcg
    32161 cattctattt tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat
    32221 gaagagatcg tggaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat
    32281 attgatgttg cgctgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtacgcc
    32341 gatatcacgc atttttccca gttctggcat tacctgaacg aacaggatga aacgcccgga
    32401 tttgccgatg acatgacctg ggattttatc agcaacgtca atagcataac ccgcaatgcg
    32461 atgctctatg acgcgttgaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg
    32521 cgttttagcg ggatggtcaa aacggcgctg acgctggcag taacgacaat cttaaaggaa
    32581 ttaacgccgt gaaaattgaa ttaacggtga atgggctgaa gatacaggca cagtaccaga
    32641 acgaagagat tgaaaatgta cataaaccgt tgctgcgtat gctggcggcc ttacagacgg
    32701 taaatccgca gcggcgtacg gtggtttttc tttgcgctcc gccggggacg ggtaaatcta
    32761 ccctaaccac cttctgggaa tatctcgcgc aacaagaccc ggagctgccc gctattcaga
    32821 cgctcccgat ggatggtttt caccattaca atagctggct ggacgcgcat caattgcgcc
    32881 ccttcaaagg cgcaccagag acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg
    32941 tggtggaagg ggattgtacg tggccgcagt acgatcgaca aaagcatgat cctgttgaag
    33001 atgcgttgca cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg
    33061 acgagaagtg gtgccagctt gcgcaatttt gtgatttctc gatctttatc aacgcgccag
    33121 ctactgcgtt gcgggagcgt ctggtggggc gcaagctggc gggaggggta tcgctggcag
    33181 atgcagaagc cttttatgac cgtaccgacg ggccgaatgt tcgccgggtg ctggaagaga
    33241 gtctgcctgc aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggtggatt
    33301 aacgttcctg attacaccat cttccagtcg caccacacga gagaaatgac gccgggtaaa
    33361 tgcggcgtca tgactgattg ctaccacgct cgtgcctcgc tggcggcact tctccagcca
    33421 gctttcaaac acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagtaa
    33481 tagtggcgga gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcactatg
    33541 cagatccaac gggtgcgagt tggcagtgtc tgctaactga caacattgca gggcagcatg
    33601 ggtacgttga gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc
    33661 gtcagcgacg gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaacctt
    33721 tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc
    33781 ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt
    33841 gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca
    33901 atccgttgcc gcgccaggcc agcgataaga tatctgattt aacgttaaca tagtggctgg
    33961 agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga
    34021 cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac
    34081 agcatttcgc tggcctgcgg cgtcaggcgg ctaaaggctt catcaagaat taagagcttt
    34141 ggctgcatcg cgatggcgca ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt
    34201 gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc
    34261 gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc
    34321 acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg
    34381 gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct
    34441 gttatctctc ctggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt
    34501 ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca
    34561 taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc taacgtaacc
    34621 atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt
    34681 gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc
    34741 gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt gttagcggaa
    34801 taattaacgc gggcatcgca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc
    34861 gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata
    34921 acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgaa
    34981 taaaacattg cacggggacg tactgcatcc acaactgtga ggttgaaaca atcgccagaa
    35041 tccgcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc gtgccgctta
    35101 gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca
    35161 gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg cagaaaaagg
    35221 tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg
    35281 ccaatgccca cagtattaat gaggtaaatg gatgcattta gcgcacggca gccattgccg
    35341 gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca atcaccgctg
    35401 taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt
    35461 tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc
    35521 cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc
    35581 cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc caaccggcga
    35641 ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc
    35701 cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg
    35761 cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt
    35821 gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa
    35881 agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg ggaaatatgc
    35941 aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta
    36001 ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc
    36061 atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg ttccagcgtg
    36121 ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcctggca
    36181 tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatccaggc ggcatgaagt
    36241 tgatgctgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc
    36301 agtgcgtaaa agcgtgcgga ggaagaatat gggtagggca atgtcatcat tggagtactc
    36361 cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt
    36421 cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt
    36481 ggaataaatt ctacaggggc ggggctgaaa acgtgacgta agtcagataa ttgctttctt
    36541 tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt
    36601 cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg
    36661 gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc
    36721 tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc
    36781 tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga
    36841 tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga
    36901 agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga
    36961 acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac
    37021 cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt
    37081 gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca
    37141 ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat
    37201 aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac
    37261 aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg
    37321 cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg ccggtatcac
    37381 acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat
    37441 aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt
    37501 caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga
    37561 attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac
    37621 ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg
    37681 gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta
    37741 agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa
    37801 ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg
    37861 taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca
    37921 tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta
    37981 ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca
    38041 aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag
    38101 gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa
    38161 ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg
    38221 ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag
    38281 gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc
    38341 cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt ttggactccc
    38401 tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg
    38461 cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagctaaac
    38521 gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct
    38581 ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc
    38641 tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca
    38701 ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa
    38761 tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc
    38821 tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg
    38881 gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc
    38941 gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta
    39001 gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga
    39061 atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc
    39121 aacttgaagt atgatgagta taaggcccga cgatacagga caagagacat gtctaagatt
    39181 tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta
    39241 gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac
    39301 gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt
    39361 ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc
    39421 ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg
    39481 gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg
    39541 gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc tcacatgatc
    39601 gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgt
    39661 atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac
    39721 ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat
    39781 tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc gtccttcggt
    39841 aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat
    39901 tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgag cttcgtattc
    39961 cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg
    40021 aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac
    40081 aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg
    40141 aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatg
//
LOCUS       DAJTYD010000013        40168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTYD010000013 DAJTYD010000000
VERSION     DAJTYD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.013"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(257..1114)
                     /gene="murI"
                     /locus_tag="OO585_000650"
     CDS             complement(257..1114)
                     /gene="murI"
                     /locus_tag="OO585_000650"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6628335.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1059..2907)
                     /gene="btuB"
                     /locus_tag="OO585_000651"
                     /pseudo
     CDS             complement(1059..2907)
                     /gene="btuB"
                     /locus_tag="OO585_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2961..3151)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3265..4365
                     /gene="trmA"
                     /locus_tag="OO585_000652"
     CDS             3265..4365
                     /gene="trmA"
                     /locus_tag="OO585_000652"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6628336.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4405..4764)
                     /locus_tag="OO585_000653"
     CDS             complement(4405..4764)
                     /locus_tag="OO585_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6628337.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4764..5414)
                     /gene="fabR"
                     /locus_tag="OO585_000654"
     CDS             complement(4764..5414)
                     /gene="fabR"
                     /locus_tag="OO585_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6628338.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5735..7135
                     /gene="sthA"
                     /locus_tag="OO585_000655"
     CDS             5735..7135
                     /gene="sthA"
                     /locus_tag="OO585_000655"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6628339.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7118..8035)
                     /gene="oxyR"
                     /locus_tag="OO585_000656"
     CDS             complement(7118..8035)
                     /gene="oxyR"
                     /locus_tag="OO585_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6628340.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8302..9675)
                     /gene="argH"
                     /locus_tag="OO585_000657"
     CDS             complement(8302..9675)
                     /gene="argH"
                     /locus_tag="OO585_000657"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6628341.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9736..10512)
                     /gene="argB"
                     /locus_tag="OO585_000658"
     CDS             complement(9736..10512)
                     /gene="argB"
                     /locus_tag="OO585_000658"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6628342.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10520..11524)
                     /gene="argC"
                     /locus_tag="OO585_000659"
     CDS             complement(10520..11524)
                     /gene="argC"
                     /locus_tag="OO585_000659"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6628343.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11678..12829
                     /gene="argE"
                     /locus_tag="OO585_000660"
     CDS             11678..12829
                     /gene="argE"
                     /locus_tag="OO585_000660"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6628344.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13181..15832
                     /gene="ppc"
                     /locus_tag="OO585_000661"
     CDS             13181..15832
                     /gene="ppc"
                     /locus_tag="OO585_000661"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6628345.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            16015..17748
                     /locus_tag="OO585_000662"
     CDS             16015..17748
                     /locus_tag="OO585_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6628346.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17963..18814
                     /locus_tag="OO585_000663"
     CDS             17963..18814
                     /locus_tag="OO585_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6628347.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18801..19142)
                     /locus_tag="OO585_000664"
     CDS             complement(18801..19142)
                     /locus_tag="OO585_000664"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6628348.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19144..20022)
                     /locus_tag="OO585_000665"
     CDS             complement(19144..20022)
                     /locus_tag="OO585_000665"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6628349.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19988..22285)
                     /locus_tag="OO585_000666"
     CDS             complement(19988..22285)
                     /locus_tag="OO585_000666"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6628350.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTPGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22336..22656)
                     /locus_tag="OO585_000667"
     CDS             complement(22336..22656)
                     /locus_tag="OO585_000667"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6628351.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22671..23750)
                     /locus_tag="OO585_000668"
     CDS             complement(22671..23750)
                     /locus_tag="OO585_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6628352.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24059..26560
                     /gene="ptsP"
                     /locus_tag="OO585_000669"
     CDS             24059..26560
                     /gene="ptsP"
                     /locus_tag="OO585_000669"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6628353.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSSILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26572..27234
                     /gene="fsa"
                     /locus_tag="OO585_000670"
     CDS             26572..27234
                     /gene="fsa"
                     /locus_tag="OO585_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6628354.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27245..28348
                     /gene="gldA"
                     /locus_tag="OO585_000671"
     CDS             27245..28348
                     /gene="gldA"
                     /locus_tag="OO585_000671"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6628355.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28622..29239
                     /locus_tag="OO585_000672"
     CDS             28622..29239
                     /locus_tag="OO585_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6628356.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29266..30171)
                     /gene="yijE"
                     /locus_tag="OO585_000673"
     CDS             complement(29266..30171)
                     /gene="yijE"
                     /locus_tag="OO585_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6628357.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30265..32445)
                     /gene="katG"
                     /locus_tag="OO585_000674"
     CDS             complement(30265..32445)
                     /gene="katG"
                     /locus_tag="OO585_000674"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6628358.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32774..33664)
                     /gene="metF"
                     /locus_tag="OO585_000675"
     CDS             complement(32774..33664)
                     /gene="metF"
                     /locus_tag="OO585_000675"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6628359.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(34013..36445)
                     /gene="metL"
                     /locus_tag="OO585_000676"
     CDS             complement(34013..36445)
                     /gene="metL"
                     /locus_tag="OO585_000676"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6628360.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLPTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36448..37608)
                     /gene="metB"
                     /locus_tag="OO585_000677"
     CDS             complement(36448..37608)
                     /gene="metB"
                     /locus_tag="OO585_000677"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6628361.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37885..38202
                     /gene="metJ"
                     /locus_tag="OO585_000678"
     CDS             37885..38202
                     /gene="metJ"
                     /locus_tag="OO585_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6628362.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38386..38994
                     /locus_tag="OO585_000679"
     CDS             38386..38994
                     /locus_tag="OO585_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6628363.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39139..39814
                     /locus_tag="OO585_000680"
                     /pseudo
     CDS             39139..39814
                     /locus_tag="OO585_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39986..40159)
                     /locus_tag="OO585_000681"
     CDS             complement(39986..40159)
                     /locus_tag="OO585_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628364.1"
                     /translation="MGNDNIFLSNSDSAALYCVAKIFPDGENILIYKVNIINASSKKD
                     VFFMKAQHQPTMT"
BASE COUNT         9864 a        11068 c        10047 g         9189 t
ORIGIN      
        1 gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc ggcgcggggt
       61 gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc tctgtcggaa
      121 ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg tcagtggtgg
      181 cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt ttctgaccgc
      241 gcaacattca accaaatcag cctaaaactg ccagtttttc gagcgtttcg aagccgtaac
      301 gctgtaaaac gggcaataat tgttcagctt ctggcgtcat tgccatacaa aaggcaatat
      361 tcgcatcggc agattttgca tccggggctt catgttctaa caaccaggcc gttcggcgag
      421 caatcgctgc gccagaatcc actagccggg ttccctctgg cagcacttgt aacagttctt
      481 cttgtagtag agggaaatgg gtgcaaccca atacaacggt atctggcggc tctttcattc
      541 ttaaccacgg gcgtaggata cgttttagtg catccagaga aacatcttcg ccatgtagct
      601 tcgcttcagc caactcaacc atctctgccg agcccagcat ttctatctgg cattcattag
      661 cgaaacgcgc gatcagctca tgagtataag aacgtttaac tgttccgcgg gttgccagta
      721 atccgacaat gccatttgcc gtcagacgtg cagcaggttt aatcgccggc acgacaccaa
      781 caaccgggaa gtcgaacttt tcgcgtaatg caggaagtga aacggtactg gcagtgttgc
      841 aagcgaccac agccagcgca aggggataac gttcttgcac cgcggtgaca attgccacca
      901 ctcgctcaac aataaacgct tcgctttttt cgccatacgg gaaagcgacg ttatcgaaag
      961 cataaatgta atggagatcc ggtaagagat gccggatctc gtcatagacc gacaacccac
     1021 cgacgccgga gtcaaacacc agcacggtgg gacgtggttc agaaggtgta gctgccagac
     1081 aaggtgtatt cccgtcctgc agtttggtag ccatagactg tctcataatc tttgtcgaac
     1141 aggttggcta ttttaccacg aactgtcagg tgagaggtga ccggatacgc aaccgcaaga
     1201 tcccacaagc tcacaccgcc cattttaacg gtttgataag gataagatga gtaatcctta
     1261 tcatagcgag tgcctaaata ctgataagta ataccccagt cgaagtcata caactgccag
     1321 tcgagctggt atttcacctg ctgtttagca cggcgtaaca acggcgtgtc ggtaatcgca
     1381 ttgcgcgcat cgacataatc ataactcaca gtatgcgtca gtggtccggt atcaaaattg
     1441 gcggtcgcct cgacgccctt aatccgcgct ttcccttcgt tgtaatattt cagggtgtga
     1501 tcatcataat cgatcaagtc actgacatcg ttacgatatc cggaaatacg ccagttcacc
     1561 ccagcggtta agccttcaaa cgcgccttcc cactgtttgc ttttctccgg gtccagattc
     1621 ggatttccgt agaagccata cagttgcccc agatttggtg ccttataaga tgtcccgtag
     1681 gaagcaatga agcgataacc ttcgatgaat tcccaaccgg cgctggtttg ccaggttcca
     1741 tgacgaccaa actgtgagtt atcgtcactg cgtgctgcgc cttcaaaggt aaaatcgccg
     1801 acttgttgca gcccggtcag atagatgccg gtattacgtt gatcatatcc atcctcaaca
     1861 taacctgtac ccggcgtcgt agtctgtttc tgccagtcga cacccgcacc aatactaccg
     1921 tgaccaacga tgacattgtt tgcccactgg acggtgtatt gcttcatctc atcgagcgtc
     1981 gccgacgaat cataacgacc ataatgggga tcgtagttgt aatctttgct atggctatag
     2041 ctggtaatga gttgtgattt aatcagttcg ccgttatagc gcagcccggc gtcccaactt
     2101 tggctataga gtttacggat atcgagcaac ggtgaaccgg gagaataata cgcgtcataa
     2161 ttggtacggt tatcatagcc atagccgcgc acaaagccgc tccaggcatc agtaaagtta
     2221 tgctccagcg cgccataaag cgttttactt aaaaaaacat cgttatctgt ctgcgcttgc
     2281 gttccggtat taccataggc aacaacatca taaccatgag tatgggcata atcgcccaac
     2341 agcgttaccc gtgtcttatc ccccagttgt tgctgcgtag agacatcata gttctgataa
     2401 ctattgcttc cccaccctgc tgaaatttcc gttccggggt catcgcgcgt cgtgatgata
     2461 ttcaccaccc cgcctattgc atcggaacca taaacagcgg agcgcggccc acggatatat
     2521 tcaacacgct ggacaagcgc acgcaatagg gaactggcta aggtcggcag aaccactcac
     2581 ccccgccaga ttcaggcgta cgccatcaat taacaccaac acatgactgg catttgtacc
     2641 gcgaataaaa atagatgaga gctgacctga accgccgttt tgggtgatat cgacgcccgg
     2701 aagacggcgc agcacatcat tgaccgaggt cgactgccag cggtcgatat cctgacgggt
     2761 cacaacggtg gttggtgcaa gcacagtgct gcgcggctgt tcaaaacggt tagcagtaac
     2821 gacgagagta tccgggctgg tatcctgtgc ccaagcggaa aaggctgtga cagaacacgc
     2881 cgtcagcagc gaagcttttt taatcattgt aaagcatcca caatagaaga aggatgccgc
     2941 aggtttcatc aatattacgc gatgatgaga cgttggccgg caggtcttcg ggcttggagg
     3001 ggtatctaag atactaagag atgatgactt cccaccggat ggcagtgtcc gcataacgca
     3061 atcatcgcac ctttccttac cgctgcgcgt cagctccaga ttcgcactgg attccctatt
     3121 aactcacagg accggcaagt ggatgctaca ggttgtaaca agttactgtc cagacgtagc
     3181 tcacaaatag gaattcatca agatctggac atctgatgag caatccctac aatcgccgcg
     3241 tactttaatt tttcaggata catcatgacc cccgaacacc ttccaacaga acagtatgaa
     3301 gcgcagttag ccgaaaaagt ggtacgtttg caaagtatga tggcaccgtt ttctgacctg
     3361 gttccggaag tgtttcgctc gccggtcagt cattaccgga tgcgtgcgga gttccgcatc
     3421 tggcacgatg gcgatgacct gtatcacatc attttcgatc aacaaaccaa aagccgtatc
     3481 cgcgtggata gcttccccgc cgccagtgaa cttatcaacc agttgatgac ggcgatgatt
     3541 gcgggtgtgc gtaataatcc cgttctgcgc cacaagttgt tccagattga ttacctcacc
     3601 acgctgagta atcaggcggt ggtttccctg ctataccata agaagctgga tgatgagtgg
     3661 cgtcaggaag cggaggccct gcgcgatgca ctgcgcgcgc agaatctgaa tgtgcatctg
     3721 attggtcggg caacgaaaac caaaatcgag ctggatcaag attacatcga cgagcgtctg
     3781 ccggtcgcag ggaaagagat gatctaccgt caggtagaaa acagctttac ccagccgaac
     3841 gcggcgatga atattcagat gctggaatgg gcgctggacg taaccaaagg ctcaaaaggc
     3901 gatttactgg agctgtactg cggcaacggt aacttttcat tagcgctggc gcgcaatttt
     3961 gatcgggtat tagccaccga aatcgctaag ccgtcggttg ctgctgcgca atacaacatc
     4021 gcagctaacc atattgataa cgtacaaatt attcgtatgg cggcagaaga atttactcag
     4081 gcgatgaatg gtgtgcgcga gtttaaccgc ctgcaaggga tcgacttaaa gagttatcag
     4141 tgcgaaacca tttttgtcga cccaccgcgc agcggtctgg acagtgaaac cgagaaaatg
     4201 gtgcaggcgt atccgtgtat tttgtacatc tcctgtaacc cggaaacgtt atgcaagaat
     4261 ctggaaacat taagccagac gcacaaggtc gaacgtctgg ctctgtttga tcagttcccc
     4321 tacacgcacc atatggagtg cggcgtatta ctgaccgcga agtaaaaccc atgccggatg
     4381 cgccagcatc cggcataata ccgattactc agcaacttcc tgcttacggt tacgcatctt
     4441 cgcgccaatc cagaacacca taatgacgga gagtactgcc gggaagaagt tagagccgat
     4501 atccggatat tccgcacgaa ctaccgtgct gtacagcagt acgccgagaa taaaacaggc
     4561 agctgccaga cccggcaagc ctaccggcat agtgcgatta agataacgtt gatgcaggca
     4621 gtaaaccgtc agcaccaggg aaataatcgg gaatacagaa aatggcacaa tggagctaaa
     4681 cagcgctgcg aaagtaccat taatcgataa gccagcaact aacgccagca gcagcgtacc
     4741 tctatcttga tttgcttgtt tcattactcg tccttcacat ttcccggaat aattgcggtt
     4801 ttctcttgtt cacggcgata ccagtaataa gcccctttcg aaatcattcg cagttgcagt
     4861 accagtcgct cttctaattg ccgacgttgt tcgacgccga cgtccaacgc ctcggcaccc
     4921 gcactgaaga caattgtcac cattgcttcg gcttgcgctt cagtaaacgc acgcggcata
     4981 tggttttcga gttccagata gtccgcaagt tccgcaatga agtgctgaat ttcacgcgca
     5041 acggcggcac gaaacgcagc ggaggtgccg gagcgttccc gcaataataa ccggaaggcg
     5101 ttaggattat taccgatgaa ctccataaat gtggagaccg aggtgcggat cacactcccg
     5161 cctttggcga tacgctgacg cgcctggcgc atgagttggc gtagcattaa accgctctca
     5221 tcaaccatgg tcagacccag ttcgtctacg tcgcggaaat gccgataaaa agaggtggga
     5281 gcaatgcccg cttcacgcgc cacttcacgc aaactcaggc tggcgaagct gcgttcagca
     5341 cttaattggc taaatgcggc ttccaccagc gaacggcggg ttttttcttt ttgttgcgct
     5401 cttatgccca tcacgatgtc tgaatccttg ccaaaagtac tgctggcact ataccagaga
     5461 atgaacatac tggattgctc cagtattgtg aatgaacggt aacgcaataa aacatattta
     5521 ctggccggtt cttttacgta cagcggaaac ctgccgctta aacggagagt atcgtcgata
     5581 aaaatccaat aaaacgtcag ggcaaaagta agaaacagac aaagcaaagg ccgctcagga
     5641 tatagccaga taaatgacgg ggatcaattg gcttacccgc gataaaatgt taccattctg
     5701 ttgcttttat gtataagaac aggtaagccc taccatgcca cattcctacg attacgatgc
     5761 catagtaata ggttccggcc ccggcggcga aggcgctgca atgggcctgg ttaagcaagg
     5821 tgcgcgcgtc gcagttatcg agcgttatca aaatgttggc ggcggttgca cccactgggg
     5881 caccatcccg tcgaaagctc tccgtcacgc cgtcagccgc attatagaat tcaatcaaaa
     5941 cccactatac agcgaccatt cccgactgct ccgctcttct tttgccgata tccttaacca
     6001 tgccgataac gtgattaatc aacaaacgcg catgcgtcag ggattttacg aacgtaatca
     6061 ctgtgaaata ttgcagggga acgctcgctt tgttgacgag catacgttgg tgctggattg
     6121 cccggacggc agcgtcgaaa cactaaccgc tgaaaaattt gttattgcct gcggctctcg
     6181 tccatatcat ccaacagatg ttgatttcac ccatccacgc atttacgaca gcgactcaat
     6241 tcttagcatg caccacgaac cgcgccatgt acttatctat ggtgctggag tgatcggctg
     6301 tgaatatgcg tcgatcttcc gcggtatgga tgtaaaagtg gatctgatca acacccgcga
     6361 tcgcctgctg gcatttctcg atcaagagat gtcagattct ctctcctatc acttctggaa
     6421 cagtggcgta gtgattcgtc acaacgaaga gtacgagaag atcgaaggct gtgacgatgg
     6481 tgtgatcatg catttgaagt cgggtaaaaa actgaaagct gactgcctgc tctatgccaa
     6541 cggtcgcacc ggtaataccg attcgctggc gttacagaac attgggctgg aaactgacag
     6601 ccgcggacag ctgaaggtca acagcatgta tcagaccgca cagccgcacg tttacgcggt
     6661 gggcgacgtg attggttatc cgagcctggc atcagcggcc tatgaccagg ggcgcattgc
     6721 cgcgcaggcg ctggtaaaag gcgaagccac cgcacatctg attgaagata tccctaccgg
     6781 catttacacc atcccggaaa tcagctctgt gggcaaaacc gaacagcagc tgaccgcaat
     6841 gaaagtgcca tatgaagtgg gccgcgccca gtttaaacat ctggcacgcg cacaaatcgt
     6901 cggcatgaac gtgggcacgc tgaaaatttt gttccatcgg gaaacaaaag agattctggg
     6961 tattcactgc tttggcgagc gcgctgccga aattattcat atcggtcagg cgattatgga
     7021 acagaaaggt ggcggcaaca ctattgagta cttcgtcaac accaccttta actacccgac
     7081 gatggcagaa gcctatcggg tagctgcgct aaatggctta aaccgcctgt tttaacactt
     7141 tatcgaaatg gccatccatt cttgcgcgga tggcctctgc cagctgctca tagcggctgc
     7201 gcagcggtga gccaggacga taaaccaggc caatcgtgcg gcgaggttcc ggcttaatac
     7261 acggcaaata aacaacccca tcgcgtttgc gctccggcgg cacagccagc gcaggcagta
     7321 aagtgatccc gctacctgcc gccaccatgt tacgcagtgt ctccaggctg gtcgcgcgga
     7381 agtgtgtatc ttcatccgcc ccggcttcaa agcagaaacc cattgcctga tcgcgcaaac
     7441 agtgaccatc ttccagcatc agcagttttt cccctgccag atcggccatc ggtacgcatt
     7501 cgcggttcgc ccacgggtga tcttcataga tagccagcaa cattggctca tcaaacaacg
     7561 gcacttcaat gaatgcttcg ctctctttca ccagcgcgag gatcacgcaa tcgagtttgc
     7621 cgctgtccag ttgcgccagt aactggtggg tctgtgcttc atgcagatac atttccagct
     7681 ttggaaatgt ctggtgcagc atcgggataa tatgcggcag caggtacggt ccaaccgtag
     7741 gaatcaaacc aatatgcagc ggtccggaca tcgtctcgcc ctgctggctt gccatctctt
     7801 taaggacttt cacctcacgc agcacggtac gcgcctgatc caccagcagc attcccgcct
     7861 gggtgaacaa cactttacgg ctggtccgct ccagcaacat cacgcccagc tcatcttcca
     7921 gcttacgaat ttggccgcta agcgtcggct ggctaacgtg gcaggaatct gccgcacgcc
     7981 gaaaatggcg gtgttcagcc aatgccacca ggtactcaag atcacgaata ttcattatcc
     8041 atcctccatc gccacgatag ttcatggcga taggtagaat agcaatgaac gattatccct
     8101 atcaagcatt ctgactgata attgctcaca gaaacggagc ggcacctctt ttaacccttg
     8161 aagtcactgc ccgtttcgag agtttctcaa ctcgaataac taaagccaac gtgaactttt
     8221 gcggatctcc aggatccgct tttttttgcc ataaaaaagc ccggcgataa gccaggctca
     8281 aatttgtaca tataaatgtt cttaccccaa ccgcgcctgc gcaaaagcaa tcgcttgggc
     8341 cacctgctgc ggtgagacgc cgccttttgc cgcacgcttg tcgaggcacg attgtagcga
     8401 cagaatcgga tagacatctt cgccaatcac gtggctgaat ttctgcaact cgtcgagcgg
     8461 cagatcttcc agcggtttgc cctgacgaat ggcttccacc accgcttcac ccacgatatg
     8521 gtgcgcctcg cggaaaggta cgcctttcgc caccaggtaa tccgccagtt cggtggcgtt
     8581 ggcgtaaccc tgctgagccg cttcctggca acgtggacgt ttcacctgaa tgccgtccag
     8641 caccagcgcc gccatatgca ggcagtccag ccaggtatcg aacgcgtcga acagaccttc
     8701 tttgtcttcc tgcatatctt tgttgtaagc cagcggcaaa cctttcagcg tcatcatcat
     8761 gccggttaac gcaccctgca cccgaccgca tttaccgcga atcagctcca gcgcatccgg
     8821 gtttttcttc tgcggcatta atgatgaacc ggaagtcacg cggtcagaaa gctccacaaa
     8881 ccccgcttcg ccggtgttaa agaaaatcag atcttcagca aaacgcgaca gatgcaccat
     8941 gccgatagcg gcagccgaca gcagttccaa cacatggtca cggtcagaaa cgctgtccag
     9001 actgttacgg gtcgccgaag caaagcccag ccagcctgct aactgttcac ggtcgatttc
     9061 ataggccgtt cccgccagcg cgccacagcc taatgggctg acatccagac gcttaagcgc
     9121 atcctgcaaa cggctttcat cacgcgccag catctcaaca taggccaggc accagtgcgc
     9181 gaacgtcacc ggctgggcgc gttgcaggtg agtgtaacct ggcattaccg cgtcctgatt
     9241 gttttgtgcg gtttccacca gcgccgattg cagctgccgg ttagccgtca gtaactcgct
     9301 aacggtatct ttgcaccaca gtttcaggtc agtcgctacc tgatcattac ggctacgccc
     9361 ggtatgcagc tttttgccta actggcccac tttgtcgatc agtttgcctt ccacccagct
     9421 atggatatct tcggcgtcgc tttcaaggat ttgttgtggc ctggcgcgaa catcttccag
     9481 caacacgttc agcgcctctt ccagttgcgc ctgctcttct gcggttaaca cgccgaccgt
     9541 taccagggct ttggaccagg ccacagagcc aacaatatcc tgctccgcca gacggtaatc
     9601 aaagcgcagt gagtcgttga attgtttgaa ccgttgatct gctgcctggg taaaacgccc
     9661 gccccaaagt gccataactc tgtttcctta ttttgaaatt caatgccgga aagctgcgcc
     9721 tccggctaac aaaacttaag ctaaaatccg cgtacccatc ggcataccgt taaacagtgc
     9781 cggaagctgc tccgcatgac gccaggaggc gatatctacc ggacggccca gcgtgcgggc
     9841 cgcatccagc gccgcgttca ctttcactat catgccgtca gtaataatgc cctgctcaat
     9901 cagttgttct gctttcgcgg cggtcatttc ggcaatgcgt tgccctttgc cgtcgagaat
     9961 gccgctgacg tcggagagca aaatcagatc cgcgcccagc gttgccgcca gcgccgttgc
    10021 cgcctggtcg gcattgacgt tcatcagttg cccttcgtct gttacgccaa tggagctgac
    10081 caccggcaga taaccgttct ccagcaagga gttgataagc ttaggcgaac ctggctgcgc
    10141 cagtccaaca tgacctaact cttcatcaag ctgggtcact ttgacgctgt cgccatcacc
    10201 gagaaacaaa cctacagccg caatctgatg tttcttcgcc catgccaaca gggttttatt
    10261 tgccgttccc gccagtgctc cggtgataat gtctatctga tcagcaggcg tcacccgcag
    10321 gccgtttttc tttttcaccg gcagattcag ccctttcatc agctcatcca ccacgcaacc
    10381 gccgccgtgc acaatcacca gcggacgctg atgtgactca cgataattca ccagtgcgct
    10441 aaacagacgt tccagcgcct cttcactatc cagcagtacg ccgcccagtt tgataattaa
    10501 tggattcatc attgcaccct taaataagag actgcgtttc cgcatagccg aaacgaatat
    10561 tggcgcactg taccgcttgt gccgccgcgc ctttcagtaa gttgtcttcg gtcgccacaa
    10621 taatcagatg ctcgccctga acggcaaacc cgatatcgca aaatggcagc ccaacgacat
    10681 ttttcagcgc cggaacgcct ttgtcataca gccgcaccag cggtttatgg gcatacgcct
    10741 gctgtaacac ttgcgcgact tgcgcctggg tcacacccga tttcaggcgg caggtaatgg
    10801 tttcgagaat gccgcgcggg aaattgccca gatgtggggt gaagataacg tcagcaccga
    10861 ggtgtgtggc gatctctggt tgatggcgat gagtaaagac gccatacggt tgcaggctaa
    10921 cttcacaaaa actatttgaa atcgctgcct tacgtcctgc accgctcacg ccgctggtgg
    10981 cgttaatcgc cggccactga ttgaggtcaa gaagatcggc atcaatcaac ggtttcagcg
    11041 ccagctgtgc cgccgtcgga taacagcctg gcaccgcaat caaattcgct tcttttaatt
    11101 tattaccgca ccactccgcc agaccgtagg ctgcctgttc cagcagttcc gggtattgat
    11161 gggtaaagcc gtaatatttt tcatagaagg tggcgtcgtt aacacgaaac gcgccggaaa
    11221 ggtcgaacac cacgcagccc gcttcaagaa actgcggcgc taaatcgtgg ctaacttcat
    11281 gggcggtggc gagaaacact acgtccaccc ctgggctaaa ctcgctgata tccgacatcg
    11341 gctgcaacgg cagctcaacg atgcctttta gctgcggatg caaatcggag attaactttc
    11401 ccgcatcatt gctttgcgct gaaacagtca aagcggttat gttcatatgc ggatggcgat
    11461 ttacataggt cactagctct gcgccagcgt agccgctggc acccacaatc agcgtattca
    11521 acatcggggc tattcacctt cttatgtctg gttgccaggt taaacgtaaa acattcacct
    11581 tacggctggt gggttttatt acgctcaacg ttagtgtatt tttattcata aatactgcat
    11641 gaatattgat actatcatga ccagaggtgt gtcaacaatg aaaaacaaat taccgccatt
    11701 tatcgagatt taccgcgctc tgattgccac accttcaata agcgctacgg aagaggcact
    11761 cgatcaaagc aatgcagatt taatcactct gctggcggac tggtttaaag atttgggctt
    11821 caatgtggaa gtgcagcctg ttccaggaac tcgcaacaaa ttcaatatgc tggcaagtat
    11881 cggacagggg gctggcggct tgttgctggc ggggcatacc gatacggtgc catttgatga
    11941 cggtcgctgg acgcgcgatc cgtttacact gacggagcat gacggcaagc tttacggctt
    12001 aggcaccgcc gacatgaaag gcttttttgc gtttatcctt gatgcgctac gcgatatcga
    12061 cgtcacgaaa ctgaaaaaac cgctctacat tctggcgact gctgatgaag aaaccagtat
    12121 ggccggagcg cgttattttg ccgaaactac cgccctgcgc ccggattgcg ccatcattgg
    12181 cgaaccgacg tcactacaac cggtacgcgc acataaaggt catatctcta acgccatccg
    12241 tattcagggt cagtcggggc actccagcga tccagcacgc ggagttaacg ctatcgaact
    12301 aatgcacgac gccatcgggc atattttgca attgcgcgat aacctgaaag aacgttatca
    12361 ctacgaagca tttaccgtgc cataccctac gctcaacctc gggcatattc acggtggcga
    12421 cgcttctaac cgtatttgcg cttgctgtga gttgcatatg gatattcgtc cgctgcctgg
    12481 catgacactc aatgaactta atggtttgct caacgatgca ttggctccgg tgatcgaacg
    12541 ctggccgggt cgtctgacgg tcgacgagct gcatccgccg atccctggct atgaatgccc
    12601 accgaatcat caactggttg aagtggttga gaaattgctc ggagcaaaaa ccgaagtggt
    12661 gaattactgt accgaagcgc cgtttattca aacgttatgc ccgacgctgg tgctcggtcc
    12721 gggttccatc aatcaagctc atcagcctga tgaatatctg gagacgcggt ttattaaacc
    12781 cacccgtgag ttgattatcc aggtaattca ccatttttgc tggcattaaa acgtaggccg
    12841 gataaggcgc tcgcgccgca tccggcaccg ttgccaaact ccggtgccgc aataatgtcg
    12901 gatgcgatgc ttgcgcatct tatccgacct acacctttgg tgttatgtat ggcgattttt
    12961 taacatttcc ataagttacg cttatttaaa gcgtcgtgaa tttaatggcg taaattcctg
    13021 ctatttattc gtttgctgaa gcgatttcgc agcatttgac gtcaccgctt ttacgtggct
    13081 ttataaaaga cgacgaaaag caaagcccga gcatattcgc gccaatgcga cgtgaaggat
    13141 acagggctat caaacgataa gatggggtgt ctggggtaat atgaacgaac aatattccgc
    13201 attgcgtagt aatgtcagta tgctcggcaa agtgctggga gaaaccatca aggatgcgtt
    13261 gggagaacac attcttgaac gcgtagaaac tatccgtaag ttgtccaaat cttcacgcgc
    13321 tggcaatgat gctaaccgcc aggagttact caccacctta caaaatttgt cgaacgacga
    13381 gctgctgccc gttgcgcgtg cgtttagtca gttcctgaac ctggccaaca ccgccgagca
    13441 ataccacagc atttcgccga aaggcgaagc tgccagcaac ccggaagtga tcgcccgcac
    13501 cctgcgtaaa ctgaaaaacc agccggaact gagcgaagac accatcaaaa aagcagtgga
    13561 atcgctgtcg ctggaactgg tcctcacggc tcaccctacc gaaattaccc gtcgtacact
    13621 gatccacaaa atggtggaag tgaacgcctg cttaaaacag ctcgataaca aagatatcgc
    13681 tgactacgaa cacaaccagc tgatgcgtcg cctgcgccag ttaatcgccc agtcatggca
    13741 taccgatgaa atccgtaagc tgcgtccaag cccggtagat gaagccaaat ggggctttgc
    13801 cgtagtggaa aacagcctgt ggcaaggcgt accaaattac ctgcgcgaac tgaacgaaca
    13861 actggaagag aacctcggct acaaactgcc cgtcgaattt gttccggtcc gttttacttc
    13921 gtggatgggc ggcgaccgcg acggcaaccc gaacgtcact gccgatatca cccgccacgt
    13981 cctgctactc agccgctgga aagccaccga tttgttcctg aaagatattc aggtgctggt
    14041 ttctgaactg tcgatggttg aagcgccccc tgaactgctg gcgctggttg gcgaagaagg
    14101 tgccgcagaa ccgtatcgct atctgatgaa aaacctgcgt tctcgcctga tggcgacaca
    14161 ggcatggctg gaagcgcgcc tgaaaggcga agaactgcca aaaccagaag gcctgctgac
    14221 acaaaacgaa gaactgtggg aaccgctcta cgcttgctac cagtcacttc aggcgtgtgg
    14281 catgggtatt atcgccaacg gcgatctgct cgacaccctg cgccgcgtga aatgtttcgg
    14341 cgtaccgctg gtccgtattg atatccgtca ggagagcacc cgccataccg aagcgctggg
    14401 cgagctgacc cgctacctcg gtatcggcga ctacaaaagc tggtcagagg ccgacaaaca
    14461 ggcgttcctg atccgcgaac tgaactccaa acgtccgctt ctgccgcgca actggcaacc
    14521 aagcgccgaa acgcgcgaag tgctcgatac ctgccaggtg attgccgaag cgccgcaagg
    14581 ctccattgcc gcctacgtga tctcgatggc gaaaacgccg tccgacgtac tggctgtcca
    14641 cctgctgctg aaagaagcgg gtatcgggtt tgcgatgccg gttgctccgc tgtttgaaac
    14701 cctcgatgac ctgaacaacg ccaacgatgt catgacccag ctgctcaata tcgactggta
    14761 tcgcggcctg attcagggca aacagatggt gatgattggc tattccgact cagcaaaaga
    14821 tgcgggcgtg atggcagcgt cctgggcgca atatcaggca caggatgcat taatcaaaac
    14881 ctgcgaaaaa gcgggtattg agctgacgtt gttccacggt cgcggcggtt ccattggtcg
    14941 cggcggtgca cctgcccatg cggcactgct gtcacaaccg ccaggaagcc tgaaaggcgg
    15001 cctgcgcgta accgaacagg gcgagatgat ccgctttaaa tatggtctgc cagaaatcac
    15061 cgtcagcagc ctgtcgcttt ataccggggc gattctggaa gccaacctgc tgccaccgcc
    15121 ggagccgaaa gagagctggc gtcgcattat ggatgaactg tcagtcatct cctgcgatgt
    15181 ctaccgcggc tacgtacgtg aaaacaaaga ttttgtgcct tacttgcgct ccgctacgcc
    15241 ggaacaagaa ctgggcaaac tgccgttggg ttcacgtccg gcgaaacgtc gcccaaccgg
    15301 cggcgtcgag tcactacgcg ccattccgtg gatcttcgcc tggacgcaaa accgtctgat
    15361 gctccccgcc tggctgggtg caggtacggc gctgcaaaaa gtggtcgaag acggcaaaca
    15421 gagcgagctg gaggctatgt gccgcgattg gccattcttc tcgacgcgtc tcggcatgct
    15481 ggagatggtc ttcgccaaag cagacctgtg gctggcggaa tactatgacc aacgcctggt
    15541 agacaaagca ctgtggccgt taggtaaaga gttacgcaat ctgcaagaag aagacatcaa
    15601 agtggtgctg gcgattgcca acgattccca tctgatggcc gatctgccgt ggattgcaga
    15661 gtctattcag ctacggaata tttacaccga cccgctgaac gtattgcagg ccgagttgct
    15721 gcaccgctcc cgccaggcag aaaaagaagg ccaggaaccg gatcctcgcg tcgaacaagc
    15781 gttaatggtc actattgccg ggattgcggc aggtatgcgt aataccggct aatcttcctc
    15841 ttctgcaaac cctcgtgctt ttgcgcgagg gttttctgaa atacttctgt tctaacaccc
    15901 tcgttttcaa tatatttctg tctgcatttt attcaaattc tgaatatacc ttcagatatc
    15961 cttaaggaat tgtcgttaca ttcggcgata tttttttcaa gacaggttct tactatgcat
    16021 tccacagaag tccaggctaa accccttttt agctggaaag ccctgggttg ggcactgctc
    16081 tacttttggt ttttctctac tctgctacag gccattattt acatcagtgg ttatagtggc
    16141 accaacggca ttcgcgactc gctgttattc agctcgctgt ggttgatccc ggtattcctc
    16201 tttccgaagc ggattaaaat tattgccgca gtaatcggcg tggtgctatg ggcggcctct
    16261 ctggcggcgc tatgctacta cgtcatctac gggcaggagt tctcgcagag cgttctgttt
    16321 gtgatgttcg aaaccaacac caacgaagcc agcgagtatt taagccagta tttcagcctg
    16381 aaaattgtgc ttatcgcgct ggcctatacg gcggtggcag ttctgctgtg gacacgcctg
    16441 cgcccggtct atattccaaa gccgtggcgt tatgttgtct cttttgccct gctttatggc
    16501 ttgattctgc atccgatcgc catgaatacg tttatcaaaa acaagccgtt tgagaaaacg
    16561 ttggataacc tggcctcgcg tatggagcct gccgcaccgt ggcaattcct gaccggctat
    16621 tatcagtatc gtcagcaact aaactcgcta acaaagttac tgaatgaaaa taatgccttg
    16681 ccgccactgg ctaatttcaa agatgaatcg ggtaacgaac cgcgcacttt agtgctggtg
    16741 attggcgagt cgacccagcg cggacgcatg agtctgtacg gttatccgcg tgaaaccacg
    16801 ccggagctgg atgcgctgca taaaaccgat ccgaatctga ccgtgtttaa taacgtagtt
    16861 acgtctcgtc cgtacaccat tgaaatcctg caacaggcgc tgacctttgc caatgaaaag
    16921 aacccggatc tgtatctgac gcagccgtcg ctgatgaaca tgatgaaaca ggcgggttat
    16981 aaaaccttct ggatcaccaa ccagcagacg atgaccgccc gcaataccat gctgacggta
    17041 ttttcgcgcc agaccgacaa gcagtactac atgaaccagc aacgtacgca gagtgcgcgt
    17101 gaatacgaca ccaacgtgct gaagccgttc caggatgtgc tgaatgaccc tgcgccgaag
    17161 aaactgatca tcgttcatct gctgggtacg catatcaaat acaaataccg ctacccggaa
    17221 aatcagggca agtttgatgg caataccgat catgttccgc caggattaag cgcagaagag
    17281 ctggaatcat ataacgatta tgacaacgct aacttgtata acgatcatgt ggttgccagc
    17341 ctgattaaag actttaaagc ggcagacccg aacggattcc tggtttactt ctctgaccac
    17401 ggtgaagagg tttacgacac gccgccgcac aaaactcagg ggcgtaacga agacaacccg
    17461 acgcgccaca tgtacaccat tccgttcctg ctgtggacgt cggaaaaatg gcaagcgact
    17521 catccccgtg atttctcaca ggatgtcgat cgtaaataca gcctggcgga actgatccac
    17581 acctggtcag atttggcggg cttatcttac gacggttacg atccaacccg ttcagtggtg
    17641 aatccgcagt tcaaagaaac tacccgctgg attggtaacc cgtacaagaa aaacgcgctg
    17701 atcgattacg acactctgcc gtatggcgac caggtaggta atcagtaatt atcagccagg
    17761 cgctatttgc cggatgcgac gcttgacgcg tcttatccgg cctacagatc gcggcggtag
    17821 gctggataag gcgtttacgc cacatccggc aatacaactg ccgcaatgtt tacatcgaac
    17881 tcccctcagg ccacactttt caggtggcct tttttaataa tgccagttac tgttttcccg
    17941 tctacatacc taagggaata acatgtatca cgacatcagc tatctgctct cccgcctgat
    18001 caacggcccg ctgtccctgc gccagattta ctttgccagc agtaacggcc cggtgcccga
    18061 tcttgcgtat caggtcgatt ttccacggct ggaaattgtg ctggaaggtg agtttgttga
    18121 taccggcgct ggagcaacgt tagttcccgg cgatgtgctg tacgttgccg ctggtggctg
    18181 gaatttccca caatggaaaa cacccgctac cacctttagc gtgctgtttg gcaaacagca
    18241 actcggcttc agcgtcgtgc aatgggatgg caaacaatat caaaatctgg cgaagcaaca
    18301 cgtcgcccga cgcggcccac gtataggttc ttttctgcta caaacgctca atgaaatgca
    18361 aatgcagccg caggagcagc aaacggcaag gcttatcgta gccagcctgc ttagccactg
    18421 ccgcgatttg ctcggcagcc aaatccagac cgcctcacgc agccaggcac tatttgaagc
    18481 tattcgcgat tatatcgacg aacgctatgc ctccgcgctt acccgcgaat ctgtcgcaca
    18541 ggcattctat atttcaccaa actatctctc gcacctgttt caaaaaacgg gggccattgg
    18601 ttttaacgaa tacctgaatc acacgcgact ggagcacgct aagacgttat tgaaaggcta
    18661 tgacctgaag gtaaaagaag tggcgcacgc ctgcggcttt gtcgacagca actacttctg
    18721 ccgtctgttt cgtaaaaaca ccgaacgctc gccgtcggaa tatcgccgac agtaccacag
    18781 ccaactgaca gaaaaaccga ctactccaga ataagatggg tttgctgcgg cgcagaaagc
    18841 actttgcgca ccgcgctcat tacccgctgc ggctcacgca ggaatgcgta gatgctgcat
    18901 tgcacatagc ggcaatgttc aaatcgctcg gcaccggcaa gctcgatatc cgtaatcaac
    18961 agagcaacat ccgcccgacg gatatcctcg tccgctaaac gattctccgt tcccagcgcg
    19021 ccctgagttt caatgctgac gccccacttc tctaactggc acaacttttc cagccgttcc
    19081 gccgccatat aagtatgcgc cacgccgctt acgcaggcgg ttactgccac caggtatgcc
    19141 attttaacct cccacggtaa cctgaaatcc ggcccgttct gccatttcgc gcatcgttgc
    19201 cacatcggct gatgacggcg cagcaacatc cttcatcgac catgttttcc ccagcaggcg
    19261 gtatttcggc tcgccgtact gatgaaacgg caacagatgg atctgcctga tattcagcgg
    19321 gatcagcacg tccagcgcct gctgcatatt ctcccggctg agtgtgaaac cggggatcag
    19381 cggcaaacgc gggatcacgt tgacaccctc actcaccagc aaacgcaggt tctccagtac
    19441 gcgtggcagg ttcatcttca ccacatcccg cgcctgagtc gcgtccataa tttttaaatc
    19501 gaacaacact tcatcgcaca atttcgccag cggtaacagc ttgctggctg gtgcgtctcc
    19561 ggcagtttca atggcacatg agacacccca cagccgcagt cgctgtaaaa aacgggtagc
    19621 aaactccgcc tgcattaaca cttcgccgcc agaaagcgtg acgccgccgc cggacgtgcg
    19681 aaaaaaaatg tcatctttca tcacttcccg ttccagagcg tcaaggctga tatcgcgacc
    19741 aatccgttca aacgccccgg aggggcattc atccgcatca cgcaaacatt tcgcacagtg
    19801 cagacatttc gcctctctgc gtaccgtctg gattttgccg gagatcgact ccggattagc
    19861 acaccacggg caaagatgcg gacagccttt aaaaaagacc accgtacgaa tgccctcgcc
    19921 gtcattcagt gaataacgct gaatgttgaa aatgcgcgcc acatcattga ggcgcgtttc
    19981 cacaacgtta cagctgatgc gctgtccggc ggatgatgtc atcctggatc tccttcgaca
    20041 gttcgacaaa gaaggcgctg tatccggcaa cgcgcaccac cagcccggca taatcttgtg
    20101 ggcgctgttg cgcttcccac aacgtgtcgg cgttcaccac gttaaactga atatgctgca
    20161 acttaagctg ggtaaacgcc cgtaagaagt cggccagttt gcgtaatccc gcttcacctt
    20221 ccagggtcgc cggagtgaat ttcacgttca gcaacgtacc gttagacagc agcgtattat
    20281 cgagcttact gactgacttc agtaccgctg ttggcccttg ttcgtcctgg cccagcatag
    20341 gtgacaagcc gccgtctgcc agctgttctc cggcaaaacg accgtctggc gtcgcgccaa
    20401 ccaccgatcc caacgggacg tgagcagaaa cggtatacga tcccggcgtg aagtaaccac
    20461 cgcgcgggtt ctggtatttt tccacttctt tgcagtagtg gcgcaacagt tcggcgctaa
    20521 tgttatccac ctcgtcgata tcgttaccat atttctcaaa gcggttaatt aagcgagcgc
    20581 ggactttttc gccttctggc gttgcaaagt tggcttttaa taccgacagc aattcgtcaa
    20641 aacttaaacg ctgttgctca aaaaccatcc ctttcaacgc atggagagaa tcgctcaggt
    20701 tggcgatacc gatcccctgt acgccggaga agttataacg cgcgccgcca tcggtaatat
    20761 cgcgaccttt ttccagacaa tcgctgataa aagacgagag cagcggtaca ggtgcccagt
    20821 cgcgatggcc gatatcacaa atattgctgc cctcaaccat cagggtgatg tagtggctga
    20881 tcttggcgcg gatctgctcc agtaaacctt cataagtcag cgcagcattg ccttcatttt
    20941 catgcaggca gatttccatc actttcagca gattaaacat cgcgatgtca tgcaagccgt
    21001 aggttctgcc gggaatagat aattccacac agcccactac ggaatagtcg cgcgcatctt
    21061 ccagcgaaac gccacggttg aggaacgctg gtaccaccac ttcatcgtta aagatttgcg
    21121 gaataccggt gccgaggcga atggtttcgg cggttttcat caggaatggc gtgtcgatca
    21181 aggcgttagt gcgcacgccg aggttcggtt gcggtaattg cacgctttga taggcgtcaa
    21241 ggcaaaggaa cgaaagcacg ttcaccgcgc tacgtccgtt ctcggttaac ccgccgagca
    21301 gtgcggtata gccggtcggg aaacctgcga aataacgcgc gctgctggtg gagcgcaaca
    21361 gcacgatgtc gttgcatttc acccataaag attcgagcag ttctttcagg aacgccggat
    21421 cttcgcccgg ggttaaagat gcctgataga acggcaacat atactggtcg aagcgcccta
    21481 aagacagcga actggcgttg gattcgtatt gcagaatgat gttcatgtac cagaataact
    21541 ggcacgcctg ccagaacgtc tgcggcttat gttcggcgtt atggcgagag atctccgcaa
    21601 tagtcagcag ttcttcgcga cgctgggcat ctgtgcagtt tgccgccatc gtttccgcca
    21661 gtacagcgta acgcaaaatg tgtttctgcg aggcttccag cagtaacagc gctgcctgat
    21721 aaaagtgatt ctccggctgt tgctgacaat gttgctgcat ctgtgccacc aactccccca
    21781 gcccgtgatt caacaggcgt gggtaatcaa taataatgtg cccctggcct ttatccgtct
    21841 ggttaatgct gaaaatctgc gtgctggtcg cggcttttac ttcatccgtc atctgcccgt
    21901 tgatgaaatc tttcatcgaa cgtttttccc agtacgggaa caactcttca cgatagatac
    21961 gtttatcttc ttcgctgata gcaaagcggt cctgcggacg cgtcgggaat tgatccagct
    22021 ctttcagcag ccagtaaggg tccatttccg gcgacataat cccggcgcgc ggttttacgg
    22081 tgcggttacc ggcaatcagt tcttcatcac gaatcgaaat ttcaacatgt tcaaggatat
    22141 acgctgttgc tttcgcccgg cgcaatatca ccggttcgcc ttcggtttgc cgatggctgg
    22201 cggtataaag cagcgcccgc tccagcgaga tttcacgggt attggcaaac agtgcagttt
    22261 tgaggcgaga gatacgattc gtcatgagga actcctgtgg gaaagcgaac ccgcccttgc
    22321 gagcgggaga agaacttaag cggtttgcgc caggtgagct tcaattttgc tcataatggc
    22381 gtcagcacgt tttactgcat cgctgatgtt gacgcgcacg atggtcttgc cagcaaaacg
    22441 ctcttcaaac ttgatgccga tatctttggt cagaatcacc atatcagcgc tcgccacatc
    22501 ttccgcagtc agttcatttt ccagaccaat tgacccctgg gtttctactt tcacttccca
    22561 gcctttcgct ttcgcggcac tttccagcgc ctctgctgcc atataggtat gggcaacacc
    22621 tgaaggacat gcggttactg caataatttt cgtcatggct cgttcctcaa ttaattaatt
    22681 tcaaaatcca aatccaggtc gtcttctttt tcatcagtcg aagacccatt tttacgcgcc
    22741 agacttttca gcacgttcac acaaacagcc gtcaccactg ctccgacagc cactgctgcg
    22801 atataaccca gcttgccttc aaccaccggc agcacaatca gaccacccca gcctgcatag
    22861 cactgcgcac cgaccagcgc cgcagttact gcaccacaaa ctgaaccgac catgatggaa
    22921 ggaatgacac gcagcggatc ggcagcggcg aaaggaatcg ccccttcagt aacgccaaca
    22981 cagcccatca ccagtgccgc tttgccggtt tcgcgctctt ctgcggagaa atttttacga
    23041 ccaatcagcg tcgccagccc catccccagc ggcgggatac aaatacccac ggcagcgatg
    23101 accaccacgg tataaacacc ctgagcaacg caaatcagca tgaaggcata ggctactttg
    23161 ttaaccggac cgcccatatc gaacgccagc atcagaccca tgatcaccgc cagcataaca
    23221 atgctgccct gctgcatccc ctgaagccac tgagtcaggc tgttggtcaa cgcccctaca
    23281 ggctcgccca agccccacat catgatgcct gcggtaatca gtgtgccgac gataggaata
    23341 atgaagatag gcatcaccga gcgcagaact ttatgcaccg gaattttctt caggtaatgc
    23401 accacgatgc cgccgataat cccggcgatc agtgcaccaa agaagcccgc accaaagctg
    23461 ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc aatggagtaa
    23521 ccaatgtaag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc gatatcaaac
    23581 agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc atacaacatg
    23641 acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg tgaaacgccc
    23701 gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat aagctgctcc
    23761 cgggcttgcg aaagcccatg tccatatttt tgatggagga aacaataggc aatcgcaggc
    23821 ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatatcag tacaaaaatg
    23881 cgatccgcct cataacttgc gataaaccgg aagcatgccg caaaacggcg tcttgtgaga
    23941 cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt tgtcacatgg
    24001 caggccgttg ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga gagaggcaat
    24061 ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcgc gtccggcaag
    24121 ccacgttgaa acgctgtgta atactttttc atcacaaatt gagtggcata acctgcgcac
    24181 tgaccgcaag ggcaacgcca aaagcgccct tgcgctgatt ggcaccgata cgctggcggg
    24241 cgataactgc cagttactga tttccggggc cgacgaacag gaagcgcacc agcgtttaag
    24301 ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag ttaaatctga
    24361 cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta tccgcgcccg
    24421 caccgtgtgc agcggtagtg caggcggcat tctgacgccg atctcttctt tagatctcaa
    24481 tgcgctgagt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg cactggaaaa
    24541 tggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg acggtgctac
    24601 cagctcgata ctggaagctc accgatccct ggctggcgat acatctttac gtcagcactt
    24661 actgacaggc gtcagcgagg gattaagctg tgccgaagcg attgtcgcca gtgcgaatca
    24721 cttttgcgaa gagttcgccc gttccagcag cagctacctg caagaacgtg ccctggacgt
    24781 acgcgacgtc tgcttccagt tactccagca aatctacggt gagcaacgct tcccggcacc
    24841 gggcaaactg acgcagcccg ccatttgtat ggctgatgaa ctgactccca gccagttcct
    24901 cgaactggat aaaaatcacc tcaaaggtct gttactgaaa agtggcggca ccacctcaca
    24961 taccgtaatt cttgcccgtt cgttcaacat tccgacactg gttggtgtag atattgatgc
    25021 ccttactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg cgattgtggt
    25081 tgagccaggg gaagccgtgg cccgttatta tcagcaagaa gcccgcgtac aggacgccct
    25141 tcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg gtatccgcat
    25201 tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg gcaatggtgc
    25261 ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca gtgcaccggg
    25321 cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg gacgcagcat
    25381 tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctga acattccggc
    25441 agaagctaac ccgttcctcg gttatcgcgc tgtgcgtatt tatgaagaat acgcctcgct
    25501 gttcaccaca caactacggt cgatcctgcg cgcctccgct cacggcagcc tgaaaatcat
    25561 gatcccgatg atctcctcaa tggaagagat cttatgggtg aaagaaaaac tggcggaagc
    25621 caaacagcaa ctgcgtaacg aacacattcc gtttgatgag aagatccagc ttggcattat
    25681 gctggaagta ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga ttgatttctt
    25741 tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg ataacgctaa
    25801 ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg attacgccgt
    25861 gcaggcggtg catcgccagg gcaaatggat tggtctgtgc ggtgagctgg gagcgaaagg
    25921 ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga gcgcaccatc
    25981 aattccggcg gcgaaagcgc ggatgacgca acttgatagc cgtgagtgcc gcaagttgct
    26041 caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg cgcaattccg
    26101 catgacccaa caggacgcac cgctggtcac cgccgagtgc atcacgctgg aaagtgactg
    26161 gcgcagcaaa gaagaagtgc tcaaaggcat gaccgataac ctgctgctgg cgggccgctg
    26221 ccgctatccg cgtaaactgg aagccgactt atgggcgcgc gaggccgttt tctctaccgg
    26281 tctgggcttt agttttgcca ttccacacag caaatcagaa cacattgagc aatccaccat
    26341 cagcgtggcg cgtctgcaag cgccggtgcg ctggggcgat gatgaagcgc aattcatcat
    26401 tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt tctctcgcct
    26461 cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg cctctgccga
    26521 cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat catggaactg
    26581 tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt ccccattgcc
    26641 ggggtgacaa ctaacccgag cattatcgct gccagcaagg agtccatctg ggaagttctg
    26701 ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac tatgagtcgt
    26761 gacgcgcagg gtatggtgga agaagcgaag cgcctgcgcg acgccattcc gggcattgtg
    26821 gtgaaaatcc cggtgacttc cgaaggtctg gccgccatta agatgctgaa aaaagagggt
    26881 attactacgc ttggcaccgc agtgtacagt gctgcacaag ggttactggc cgcgctggca
    26941 ggggcaaaat acgttgctcc gtatgttaac cgcgtagatg cccagggcgg ggacggcatt
    27001 cgtacggttc aggagctgca agcgctgtta gaaatgcacg caccagaaag catggtgctg
    27061 gcggccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg atgtgaatcc
    27121 atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt agagtcagct
    27181 atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct ctaaaggagc
    27241 aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg atgtgattaa
    27301 tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtgg gtgacaaatt
    27361 tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg gactggtagt
    27421 agaaattgcg ccgtttggcg gtgaatgttc gcaaaatgag atcgatcgtc tgcgtggcat
    27481 cgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa ccctcgatac
    27541 tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga ctatcgcctc
    27601 taccgatgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg agtttgaccg
    27661 ctatctactg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa tcgtcgctgg
    27721 cgctcctgca cgtctgttag cggcgggtat cggcgatgcg ctggcaacct ggtttgaagc
    27781 acgcgcctgt tcccgtagcg gcgctaccac tatggcgggc ggcaagtgca cccaggcggc
    27841 gctggcactg gcagaactgt gctacaacac tctgctggaa gaaggcgaaa aagcgatgct
    27901 tgccgccgaa cagcatgtag taacccctgc gctggaacgc gtgattgaag cgaacaccta
    27961 tttaagcggt gttggttttg aaagcggcgg actggcggcg gctcatgcgg tgcataacgg
    28021 cctgaccgct atcccggacg cgcatcacta ttatcacggt gaaaaagtgg cattcggtac
    28081 gctgacgcag ctggtactgg aaaacgcgcc ggttgaggaa atcgaaaccg tagctgcatt
    28141 tagccatgcg gtaggtttgc caataactct cgctcaactg gatattaaag aagatgtccc
    28201 ggcgaaaatg cgaattgtgg cagaagcggc atgtgcagaa ggtgaaacca tccacaacat
    28261 gcctggcggc gcgacgccag atcaggttta cgccactctg ctggtagccg accagtacgg
    28321 tcagcgtttc ctgcaagagt gggaataacc tactccaaac tcccggcttg tccgggagtt
    28381 tgaacgcaaa attgcctgat gcgctacgct tatcaggcct acgcaatctc tgcaatatat
    28441 tgaatttgcg tgctttttag gccagataag gcgttcacgc cgcatccggc actctgtgcc
    28501 agtaatctga aactcctacg ttgttcagga gttttttatc cgcttctgga cattttctct
    28561 acagaacaat cgttagcccg gaaggtcgaa tctgtgactc ttattttcac ggacgaacca
    28621 gatgaaagct tcactggcgc tgctcagcct gctaacggca tttaccagcc actccttaaa
    28681 atctcccgcc gttccaccaa ccgtggtaca aatccaggcc aataccaacc ttgccatcgc
    28741 agacggtgcc agacagcaaa tcggcaacac gctattttac gatccggcgt atgtgcggct
    28801 tacctatcct ggcggtgatg tcccgcaaga acgcggtgta tgttccgatg tggtgatccg
    28861 tgcattgcgc agccagaagg tcgatttgca gaaactggtt cacgaagata tggcgaagaa
    28921 ttttgccgag tacccgcaaa aatggaagtt aaagcgcccg gacagcaaca tcgatcaccg
    28981 tcgggtgcct aatctggaaa cctggtttag ccgccacaac aaaacacgcc ccatcagtaa
    29041 aaatgccggt gactatcaag cgggcgatat tgtctcctgg cgattagata acgggctggc
    29101 gcatattggg gtggtgtcag atggcttcgc ccgcgacggt acgccactgg tgatacataa
    29161 cattggcgca ggcgcgcagg aagaagatgt gctgtttagc tggcggatgg tggggcatta
    29221 tcgttatttt gttaaataag cgatcgcccc acatgaagaa aaaattcaga tgctttttac
    29281 actgacagct tcttttttct tacggctcac cagcgccagt gccagcacaa tcagcacaat
    29341 accgctacct tcaacggccc ccggattctc accgagcagc caccaggaaa agagtacgcc
    29401 gcaaacggga acggccagcg tgcttaagct ggcaatactg gcaggcaagt ttttcaatac
    29461 aaacaaccat aagctccacg ccagcgccgt cgccagaatc gcactgtagg ccagcgccca
    29521 gaacacggtg ggctgccagt caatttcacg ttgcggcacc agtaaagcga ccacactcat
    29581 caccagcgcc gcatagagca tctgccagga tgttaacgat aataaatcca cgcgcggatg
    29641 acgggtgtat aggcgtttag caacaatcgc gctcgccccc caactaacac cggagaggat
    29701 tgccagcatg gcacttttca tcgaagagaa atcgagttgc cacggctgca acaccaaaaa
    29761 taagccgaaa gcggcaatca gaatcgcgaa atattgccca cgtcgcaggc gttcaccgag
    29821 aaacaacgcg gcgaaaatca ccacccagaa cggcatggta tagctcagga tcgccacctt
    29881 ccccgcacct ccgctgacca acgcccactg cgccagacca accatcccgc aggtttgtaa
    29941 cagggcaatg gctaaggtgt atttaaacgg cgtcgggtgc attccgcgac cacgtaataa
    30001 aaggacgatg aataaaacga gagcgccgaa aatgcagcgt aaggcggtaa agtcgaaggc
    30061 accgatgtaa ctggtgactt gcttcatgaa aatccagcta taactccaga taagtgtgag
    30121 cacaaccagg ccactgattg ccagtgggtt gctctttcct gcggcagaca tgagagatcc
    30181 agtgtgtagt tttcctgtgg acactatacg ccggtaaaga aacttcagcg actgcctgaa
    30241 agacagtcac tgaagaaggt cagattacag caggtcgaaa cggtcgaggt tcatcacttt
    30301 cacccatgcc gctacgaagt ctttaacaaa cttctcgtgg gcatcgctgc tggcgtaaac
    30361 ttccgctacc gcacgcagga cagagttaga accaaatacc agatccgcac ggctggctgt
    30421 gtatttcact tcgccggttt cgcggtcacg tccttcgaac agctctttcg attcgtcagt
    30481 cgctttccac tcgtaacgca tatccagcaa gttcacgaag aagtcattgc tcaatacgcc
    30541 aacgcggtca gtgaagacgc cgtttttgct gctatcgaag ttggcaccca gtacacgcat
    30601 cccgcccacc agcgcagtca tttccggcgc ggtcagcgtc agttgctgtg ctttgtcgat
    30661 cagcagtgac tcggtggtgg aaacgtccag acgagcgcga tagttacgga aaccgtcagc
    30721 aattggctcc agcagctcaa acatctcaat gtcagtctga tcctgacgcg catcaacgcg
    30781 acccggcgca aacggtacat gaatgctcaa acctgcggcg cttgcggctt tctcaacacc
    30841 aaccacacca gccagcacga tgatatccgc cagcgaggct ttaccagact ctttctggat
    30901 tttctccaga acaggcagag cacgaacggc tgcggcgttc acatcccagt cgcgctgcgg
    30961 cattaatgcc agacgcgcac cgttggcacc accgcgtttg tcgccaccac ggaaggtaga
    31021 agcagatgcc caggccaccg ataccagctc actaacagac agaccagaat ccgcaatcgc
    31081 gaatttcaga tcgataatgt cctgctcggt cgggttgtag atcggctgcg gcagcggatc
    31141 ttgccagatc agatcttctt tcggcacttc cggcccgatg tagcgagatt tcggccccat
    31201 atccctgtgc gtcagtttga accaggcacg ggcaaaggct tcgttgaacg cctgcggatc
    31261 gttgaggaaa cgacgagaga tcttctcgaa ctcaggatca aaacgcagcg tcaggtcggt
    31321 caccagcatt gtcggtttac gtttcttcga cggatcgaac ggatccggga taatttccgg
    31381 tgcgtctacc gcttcgaact ggattgctcc agccggactg cgggtctgta cccactcata
    31441 tttgaacagg ttctcgaaga aatagttgct ccactgggtc ggcgtctggg tccagacaac
    31501 ttccagacca gaggtaatgg catctgcgcc aacgccgctg ccgtaagtgc tcgcccagcc
    31561 taaaccttgt tcttcaatcg gtgcagcttc tggatcagga cctacatttg atgtcggacc
    31621 ggcaccgtga gttttaccca gcgtatgacc acccgcaatc agcgccacgg tttcttcgtc
    31681 gttcatgccc atgttgccga aggtcgcacg gatagctgct gccgcagaaa gcggttcgcc
    31741 gctgtgatcc gggccttccg ggttaacgta aatcagaccc atctcggttg cacccagcgg
    31801 tgctttcgcc agcgcttccg gatgacggtg agtcagccag gctttttcat caccccagtt
    31861 aacatccaga tccggttccc agacgtcttc acgaccggca ccaaaaccga aggtacggaa
    31921 gccggagttt tccagcgcca cgttacctgc gaggataaac aggtcggccc aggagatttt
    31981 ctgaccatat ttctgtttga ttggccacaa caggcgacgc gctttatcaa ggcttacgtt
    32041 atccggccag gagttcagcg gtgcaaaacg ttgctgacca cgacccgcgc cgccgcgtcc
    32101 atcgattgaa cggtaagtac ctgcgccgtg ccaggccata cgaataaaca gaccggcgta
    32161 gctgccccag tcggctggcc accacggctg agattctgtc agcagggctt tcagatcttt
    32221 tttcaggccg tagtaatcta atttgctgaa ttctttgcgg tagtcaaagt cctcacccag
    32281 cgggttagaa cgattagaat gttggtttaa caggtcaaca cggagttgat ttggccacca
    32341 gtcgcgagtg gttgtgcccg cccccgcgct ctggtcgtga ccgccctgat ggaacgggca
    32401 tttgccagtg gctgtcgtgt tatggatatc gtctgacgtg ctcatcaatg tgctcccctc
    32461 tacagtgtta ccgttacgat acacagcgtt agagagaagt tataattgaa ttaatccacg
    32521 gatgcgatag ttgagatctt acatattgtt ggttaaagag atgtaggtca aattgatcgt
    32581 aattaagctt ttttgattat gtccgaattc ggacaaatgc tttataaaaa aggggtttag
    32641 cttcatatcc ttcaagtaat gagggagaaa acaaggctga caaccttatt ttcccgccct
    32701 catttcgagg cagcattttg tgctctgttt aaaatttgtg atcactgtgt gattttcaca
    32761 aaagccacac tatttataaa ccaggtcgaa cacccagcgt atggcaaatc gcgtaactca
    32821 tttcagcgcg gttaagcgta tagaagtgga aatctttcac tccttcacgg cttaaaatct
    32881 tcaccatatc catggcaata ttcgcgccca ccagtttgcg ggtttcggca tcatcatcca
    32941 ggccgtcgaa catttgcgcc atccacgccg gaatacgcac gttggtcata tcggcaaatt
    33001 tcttcgcctg tttaaagtta gataccggca aaattcccgg aataatttcc acatcaatgc
    33061 ccgccgatac acagcggtca cgaaaacgca ggtagctttc gacatcgaag aagaactgag
    33121 taatcgcgcg gttggctccg gcatcgactt tgcgtttcag attaagcaaa tccgcctgag
    33181 cgctttttgc ttccgggtga acttccggat acgccgccac ggagatatcg aaatctgcca
    33241 cttcttttaa tagcgtcacc aggtcagaag catacatttc tggcttacca cttcccggcg
    33301 gcagatcgcc acgcagcgcc acgatatgac gaataccgtt attccagtag tcgcgtgcaa
    33361 tggtgcgcaa ctcgtcgggc gtcgcatcaa tgcaagtaag atgcggtgcc gcttccagac
    33421 cagtgcgatc tttaatgcct ttaataatgc tgtgcgtacg gtcgcgctcg ccggagttcg
    33481 cgccataggt caccgataca aacttcggtt tcaggctgct aaggcgatcg atggagttcc
    33541 acagggtctg ctccatttca ctggtacgcg gcgggaaaaa ctcgaacgaa acgttaatct
    33601 gcccctggac ttctgccagg cgctgattca gggcatcccg ctggctggcg tgaaaaaagc
    33661 tcatacctta cctcatcaat cgcttgtagt tatatgttgt gttgtgcaca tctatccgtt
    33721 tagacgtcca gatgtaaaga tgaaggaaaa gccgaagggc gtcaaccgaa aaatgaccaa
    33781 tgatggtgag gaaaaatcag ggaagatgaa aaaacttcag gatggtaaga aaaagaaaat
    33841 ccggtatgca aagcaaaccg gactgcaaat ataaactaac catgtactta ttgcttcttg
    33901 ccggattcgt cgtaaacgct taatccggcc taatcctatg ataattcaat atatttcatt
    33961 agaaaccata aacccgaaaa catgagtacc gggcattatt aaatttctga aattacaaca
    34021 actgtgccag ccggttgata tccgactgaa tcgccccggc ggtgacgtcg cgccccgcgc
    34081 caggcccacg gatcaccagc gggttgtcgc gataccagcg gctttcgatg gcaaagacgt
    34141 tatcgcacgg cagcagtgat gccagcggat ggtcttcacg caccgcctcc acgcccacac
    34201 gcgctttgcc gttggcatcg aaacgcgcca cgtagcgcag caccagcccc atttcgcggg
    34261 ccgcttccag ccgttgcacc atctgctcgt taagttcatc gccattttca aagaaatggt
    34321 cgatgctgcc gccttcgcaa tgagcaggca ccagcgattc cacacgtacc tggtccggtt
    34381 caatgttgta acccgcttca cgcgccagaa ttaccagctt gcgcattacg tctttgccgg
    34441 agaggtcgtc acgcggatca ggctcggtta agccctgctg ccacgcctga tccaccagct
    34501 cggtaaacgg cacgctaccg tcgaattgca ggaacagcca ggagagcgtg ccggagaaga
    34561 tcccgctgat cgacaaaata gtatcgccgc tgtcgatcag atcgcgcacg gtgtggttga
    34621 tcggcaagcc cgcaccgacg gtggcattgt acagccagtg acgcccggtt ttttcgaagg
    34681 cgtcgtggat ctggcgatat ttattgctgt cgctggctcc cgccagtttg ttggcgctga
    34741 taacgtggaa accgtggctg gcgaaatcaa gatactgatc agcaagctgc tggctggcgg
    34801 taacgtccag caccactaaa tcatcatacg gatgggcgcg catccacagg aacaacgact
    34861 cttcatcctg ctcaaccgct tcatcgttga agaaggctaa cgcgcggctg gcgtccagcc
    34921 cgtcatagct caacaggcta cggcggctgt ccaccacacc tgccagcaca aactcaaagc
    34981 cggtacgtgc cgaaagcgtg ctctgctcac gggcgaacag ttccagccaa cgggaaccga
    35041 tattgccctt accgaacaat accaggccga tgcgtttttc tgcgcggaag acggactgat
    35101 gcagcccctg aatcaggctt tcggtcgggc cggtgggcag tactgccacc aggctgatgc
    35161 cgtcatcgga ctgccaggta aattcgaccg gctggccttt cagttgctgc cagaagcggt
    35221 ggcaatgcag cgggttacgg gtgacgcctg caccgaccat cgccaccagc gccagcccct
    35281 gacgcaggcg cagttcgcca ggtaatcccg cttcgtcgag gattttcagc gcactgtcgg
    35341 ccacttctga ggtgtagcaa aattgcagca actggcgatc gttatgtacg ccaaccgcca
    35401 gcgggcgtac ctgcgcgcgt ttcaggattt ggtcgatctc tttatgcgcc agtttgaaat
    35461 cctgactggc gggcacctga aactcaatca aacagacatc atcgtggctg gtgacaatac
    35521 gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt ggaaccttga tccggcgtgt
    35581 agctacagcg cagttgcagg tcgatttcgc tgccagaaac cggctgtaaa gtacgggcgt
    35641 gaagaacggg agccgccagg cgcgccagtt cgctggcctc atccagacgc agcaacggca
    35701 gcaggcaggc atctttcact ttacgcgggt cggcactgta taccccggcg acgtcgctcc
    35761 agatggttac gcgagaaaca cccgccagcg caccgatttg tgtcgcggaa tagtcggaac
    35821 cgttacgccc cagcagcacc gtttcaccgg cgttgttgcg gctgataaat ccggtcacca
    35881 ccagacgttt gcccggatgt tgcaccagca gctgttgcag caacgggtaa gaaagccctt
    35941 catcaacctg cggttgtgcg gcgcgttcag cgcgtaaaaa ctcgcgggca tcaagccagg
    36001 ccgctggcag cccttgttga ttaagtaccg cagacatcag gcgtgccgac catacttccc
    36061 cgtggcccac cacttccgca tacactgcgt cgttaatacc gctgtcgagc agcgccgcca
    36121 ggcgctcaag gtcgctgaca aaagcgctaa tgaggctatc agcttcttca gcgggtagca
    36181 gaccgctaat cagatcgcac tgatagcgac gcagcgtttg ttgaacctga tgcgcagaga
    36241 gacgatcggt ctggcttagt ttcaaccagt taatcaactg gttagtggtg ctaccggcgg
    36301 cggaaaccac catcatatcg tcaggctgag agtactccgc cataatgccc gcaacacgca
    36361 aataacactt cacatcagcc agactactgc caccaaattt atgcagttga cgacctttcg
    36421 cccctgcctg cgcaatcaca ctcattttta ccccttgttt gcagcccgga agccattttc
    36481 caggtcggca attaaatctt cgccatcttc aataccggtg gagatacgca gcagcgtctc
    36541 ggagatcccg gcggcagcac gcgcttctgg tgccatgcct gcatgtgtca tggttgcggc
    36601 gtgagagatt aaactttcca ctccccctaa tgattccgcc agcgtaaaca acgacagccc
    36661 gcccaggaaa cgacgcagcg tctgctcatc gccatccagt tcaaaactca acattgcgcc
    36721 aaagcctttt tgctggcgcg cggcaatttc atgcccctga ttttccggca gcgacgggtg
    36781 atacagtttt ttcaccaacg gctgggtttg cagatatttc acaatcgcct gcgcgttgcg
    36841 ctgggccagc tccatacgcg gcaccagcgt tcgcaacccg cgtagcagca gatagctgtc
    36901 aaacgcgccg cccgtcacgc caatattgtt tgtccaccag gcaagttcag tgacaacgtc
    36961 cgggtcttta gcaatcacca cgccagccac cacgtcagag tgaccgttca gatatttcgt
    37021 acatgaatgc aacaccagat cggcacccaa tgccagcgga ttttgtaatg ccgggcttaa
    37081 gaaggtgtta tccaccacgc tcaccgcccc gacttccctt gccagatggc agattttcgc
    37141 aatatccacg acgcgtaaca atggattact tgggctttct accagtacca gtttaggttt
    37201 ttctgccagc gctgcccgta atgcctgttc atcgccttga tcaacaaaca acacgcgata
    37261 gcaaccgcgt ttcgccagac tgtcgaacag gcgatagcta ccgccgtagc agtcgtgcgg
    37321 cgcaaccagc agatcgccag gtttcaaaaa gacggtcgtt accaagtgaa tcgcggacat
    37381 gccggtatta gtaagtactg cgccagcacc gccttccagt tctgccagcg cacgctgaac
    37441 cacatcgcgc gttgggttgc cgcgacgtga gtaatcatgc gcgcgcggtt cattaaatcc
    37501 ggtaaagtta taggtgctgg aaagatggat gggagggaca acgcaaccat actgttcgtc
    37561 gtcatttaac ccgctacgca ctgcgatggt ggcctgttta cgcgtcatgt gatgaagttt
    37621 cctgggcttt gtcggtgaaa tgtcaggcac cagagtaaac attgtgttaa tggacgtcaa
    37681 tacatctgga catctaaact tctttacgta tagattgagc aaatcccaaa tagccgttaa
    37741 aattatatgc attatcacgc cgacaggtgc attacacgat gtcacggtaa cgcctgtacg
    37801 gtaaactatg cgggtttacg gtcagtaccc acataaattg tgtggtctgg tctcaattta
    37861 ttgacgaaga ggattaagta tctcatggct gaatggagcg gcgaatatat cagcccatac
    37921 gctgagcacg gcaagaagag tgaacaagtc aaaaagatta cggtttccat tcctcttaag
    37981 gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg tgaacaacct gcgtcacgct
    38041 accaacagcg agctgctgtg cgaagcgttt ctgcatgcct ttaccgggca acctttgccg
    38101 gatgatgccg atctgcgtaa agagcgcagc gacgaaatcc cggaagcggc aaaagagatc
    38161 atgcgtgaga tggggattaa cccggagacg tgggaatact aaagcaaaaa agagcggcgc
    38221 ggagtggaat cgcctgatgc gctacgctta tcaggcctac gtcatattgc aatttattga
    38281 atttgcacga acttgtaggc cggataaggc gttcacgccg catccggcat aaacaacgag
    38341 cacgttgtct gcgacccacc gctttttata catggacgtt taactatgaa aaacaggctg
    38401 ctgatcctca gcctgctggt ttctgtacct gcctttgcct ggcagccaca aaccggcgac
    38461 atcatctttc agatctctcg ctcatcgcaa agtaaagcga tccaactggc gacccattcc
    38521 gattatagcc acaccggtat gctggtgata cgcaacaaaa agccctacgt ttttgaagcg
    38581 gtcggcccgg tgaaatacac cccgctcaag cagtggatcg cccatggtga aaagggcaaa
    38641 tacgttgttc gccgcgttga aggcggactg agtgttgaac aacagcaaaa actggcgcaa
    38701 acggtaaaac gttatctcgg taaaccgtat gatttcagct tctcatggag cgacgatcgc
    38761 cagtactgtt cggaagtggt gtggaaggtt taccagaacg cgctgggaat gcgcgtgggc
    38821 gagcagcaga agttgaaaga gttcgatctc agcaacccgc tggtccaggc taagctcaaa
    38881 gaacgctacg gcaaaaatat tccgcttgag gaaacggtag tttcaccaca agccgttttc
    38941 gacgcgccac aactcaccac ggttgccaaa gagtggccgc tgttttcgtg gtaatggatg
    39001 atgaaaaaaa taattcccgc cgtagtctgt ttgttgcttt gtagttgcac gacctttccg
    39061 caaactgtcg ctccggtaaa cggtgatgcc tccaattagt tgaattaagg atataatact
    39121 cgcttttaag gttgtttcat ggtcacagtt aaccttcatt gcccccgttg ccagtccgtt
    39181 caggtttacc gtcacgggca gaacccaaaa agacatgatc gattccgcgg ccgtgactgt
    39241 caccgggtat ttcagctcag ttactgttac gagccccgta aaccgggagt taaagaccaa
    39301 atcgctgaaa tgactttcaa cggtgccggg gttcgcgata ctgcccgtac gctgaaaatc
    39361 ggcattaata cagtcatccg aactttaaaa aactctcgcc acgaagaata acgtcatccc
    39421 cggtcgctca tgctgatgtg gcactcatct gcgaacttag tcagcaatgg agctttgtcg
    39481 gcagtaaagc gcgacagcac tggctctggt acgtgtacaa cacgaaaact ggtggtgttc
    39541 tggcctacac ttttggcccc cggactgacg aaacctgtcg tgaattgctg gcgctgctta
    39601 cccttttacc atcggcatgt tgaccactga cgaatggggt agttacacca gagagttacc
    39661 gaaggaaaag catctgactg gtaagatttt tactcagcgc attgagcgca acaatctgac
    39721 cctgagaacc cgaatcaaac gtctggcccg cagaaccatc tgtttctcgc gctctgtaga
    39781 acttcatgaa aaagtcatcg gtgcttttat cgaaaatata tgttctacta attggagtca
    39841 tcacccttct gatgagcttt ccttggatga ttaccagact gaatacacag ccaaagattt
    39901 ttacctcatt ctgctctttt ttctcaaatg acatatatta tattttacat catattcata
    39961 ggcatatgac attcccccgc ccccattatg tcattgtggg ttgatgttgg gctttcataa
    40021 aaaaaacatc tttttttgaa ctagcattaa ttatatttac tttatatatt aaaatatttt
    40081 ctccatctgg aaatatttta gccacacaat ataaagcagc tgaatccgaa ttacttaaaa
    40141 atatattatc attacccata actccagc
//
LOCUS       DAJTYD010000014        40131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTYD010000014 DAJTYD010000000
VERSION     DAJTYD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.014"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(116..2437)
                     /locus_tag="OO585_000682"
     CDS             complement(116..2437)
                     /locus_tag="OO585_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6628365.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2534..3010)
                     /gene="greB"
                     /locus_tag="OO585_000683"
     CDS             complement(2534..3010)
                     /gene="greB"
                     /locus_tag="OO585_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6628366.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3238..3957
                     /gene="ompR"
                     /locus_tag="OO585_000684"
     CDS             3238..3957
                     /gene="ompR"
                     /locus_tag="OO585_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6628367.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3954..5306
                     /gene="envZ"
                     /locus_tag="OO585_000685"
     CDS             3954..5306
                     /gene="envZ"
                     /locus_tag="OO585_000685"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6628368.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5382..7004)
                     /gene="pckA"
                     /locus_tag="OO585_000686"
     CDS             complement(5382..7004)
                     /gene="pckA"
                     /locus_tag="OO585_000686"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6628369.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7383..9107
                     /locus_tag="OO585_000687"
     CDS             7383..9107
                     /locus_tag="OO585_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6628370.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9242..10120)
                     /gene="hslO"
                     /locus_tag="OO585_000688"
     CDS             complement(9242..10120)
                     /gene="hslO"
                     /locus_tag="OO585_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6628371.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10145..10546)
                     /gene="hslR"
                     /locus_tag="OO585_000689"
     CDS             complement(10145..10546)
                     /gene="hslR"
                     /locus_tag="OO585_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6628372.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10557..11225)
                     /gene="yrfG"
                     /locus_tag="OO585_000690"
     CDS             complement(10557..11225)
                     /gene="yrfG"
                     /locus_tag="OO585_000690"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6628373.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11290..13425)
                     /gene="igaA"
                     /locus_tag="OO585_000691"
     CDS             complement(11290..13425)
                     /gene="igaA"
                     /locus_tag="OO585_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6628374.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13745..14305
                     /gene="nudE"
                     /locus_tag="OO585_000692"
     CDS             13745..14305
                     /gene="nudE"
                     /locus_tag="OO585_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6628375.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14470..17022)
                     /gene="mrcA"
                     /locus_tag="OO585_000693"
     CDS             complement(14470..17022)
                     /gene="mrcA"
                     /locus_tag="OO585_000693"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6628376.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17142..17921
                     /gene="hofM"
                     /locus_tag="OO585_000694"
     CDS             17142..17921
                     /gene="hofM"
                     /locus_tag="OO585_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6628377.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17921..18460
                     /gene="hofN"
                     /locus_tag="OO585_000695"
     CDS             17921..18460
                     /gene="hofN"
                     /locus_tag="OO585_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6628378.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18444..18884
                     /gene="hofO"
                     /locus_tag="OO585_000696"
     CDS             18444..18884
                     /gene="hofO"
                     /locus_tag="OO585_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6628379.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18874..19278
                     /gene="hofP"
                     /locus_tag="OO585_000697"
     CDS             18874..19278
                     /gene="hofP"
                     /locus_tag="OO585_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6628380.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19190..20428
                     /gene="hofQ"
                     /locus_tag="OO585_000698"
     CDS             19190..20428
                     /gene="hofQ"
                     /locus_tag="OO585_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6628381.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20829..21350
                     /gene="aroK"
                     /locus_tag="OO585_000699"
     CDS             20829..21350
                     /gene="aroK"
                     /locus_tag="OO585_000699"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6628382.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21407..22495
                     /gene="aroB"
                     /locus_tag="OO585_000700"
     CDS             21407..22495
                     /gene="aroB"
                     /locus_tag="OO585_000700"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6628383.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22587..23873
                     /gene="damX"
                     /locus_tag="OO585_000701"
     CDS             22587..23873
                     /gene="damX"
                     /locus_tag="OO585_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6628384.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23980..24816
                     /gene="dam"
                     /locus_tag="OO585_000702"
     CDS             23980..24816
                     /gene="dam"
                     /locus_tag="OO585_000702"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6628385.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24834..25511
                     /gene="rpe"
                     /locus_tag="OO585_000703"
     CDS             24834..25511
                     /gene="rpe"
                     /locus_tag="OO585_000703"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6628386.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25504..26262
                     /gene="gph"
                     /locus_tag="OO585_000704"
     CDS             25504..26262
                     /gene="gph"
                     /locus_tag="OO585_000704"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6628387.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26255..27259
                     /gene="trpS"
                     /locus_tag="OO585_000705"
     CDS             26255..27259
                     /gene="trpS"
                     /locus_tag="OO585_000705"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6628388.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27528..28433
                     /locus_tag="OO585_000706"
     CDS             27528..28433
                     /locus_tag="OO585_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628389.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28450..28812
                     /locus_tag="OO585_000707"
     CDS             28450..28812
                     /locus_tag="OO585_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6628390.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28896..30059
                     /locus_tag="OO585_000708"
     CDS             28896..30059
                     /locus_tag="OO585_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6628391.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30059..31285
                     /locus_tag="OO585_000709"
     CDS             30059..31285
                     /locus_tag="OO585_000709"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6628392.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31282..32160
                     /locus_tag="OO585_000710"
     CDS             31282..32160
                     /locus_tag="OO585_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6628393.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32171..32524
                     /locus_tag="OO585_000711"
     CDS             32171..32524
                     /locus_tag="OO585_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6628394.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32536..33840
                     /locus_tag="OO585_000712"
     CDS             32536..33840
                     /locus_tag="OO585_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6628395.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33852..34937
                     /locus_tag="OO585_000713"
     CDS             33852..34937
                     /locus_tag="OO585_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6628396.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35089..35820)
                     /gene="phoB"
                     /locus_tag="OO585_000714"
     CDS             complement(35089..35820)
                     /gene="phoB"
                     /locus_tag="OO585_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6628397.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35920..36705)
                     /gene="frlD"
                     /locus_tag="OO585_000715"
     CDS             complement(35920..36705)
                     /gene="frlD"
                     /locus_tag="OO585_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6628398.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36702..37532)
                     /gene="frlC"
                     /locus_tag="OO585_000716"
     CDS             complement(36702..37532)
                     /gene="frlC"
                     /locus_tag="OO585_000716"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6628399.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37582..38604)
                     /gene="frlB"
                     /locus_tag="OO585_000717"
     CDS             complement(37582..38604)
                     /gene="frlB"
                     /locus_tag="OO585_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6628400.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38625..40013)
                     /gene="frlA"
                     /locus_tag="OO585_000718"
     CDS             complement(38625..40013)
                     /gene="frlA"
                     /locus_tag="OO585_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6628401.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
BASE COUNT         9251 a        10199 c        11048 g         9633 t
ORIGIN      
        1 ttgtggaata aaaaggccgg agatattctc cggcactttt atgtcaggca aggcgttcgt
       61 tgccagatgc ggcgtgaacg ccttatcgcg caggtttagg ggccagtccg ggcgtttaac
      121 gttttttgcc cattgccgcc gccagcgcat ccatcatcgc gctattaccg gcaggctgcg
      181 cttcacgacc gcgtggtttg gctgccgggc ggttgttttg cgggcgttca ttaccgccgc
      241 cgcgacgggc gttggtttcg ccaggctgct cgtccagacg catagtcagg gcgatacgtt
      301 tacgctgaag atccacttcc agcactttca ccttcacaat gtcgcccgct ttcaccacgg
      361 tatgcggatc ttccacaaac ttgttcgaca atgaagagat gtgaaccagg ccgtcctgat
      421 gcacgccgat atcgacaaac gcgccaaagt tggtgacgtt ggtcaccgca ccttcgagga
      481 tcatacccgg ttgcaggtcg ttcattgtct cgacgccatc ggcaaactga gcggttttaa
      541 attccggacg cggatcgcga cccggttttt ccagctcttt gatgatgtca gttactgtcg
      601 gcacaccgaa tttttcatca gtaaagtcag acgctttcag gttacgcagt tcgctgctgt
      661 tacccatcag atctttcagt gcctgctgtg ttgctgccag aatgcgttcc accaccggat
      721 aggcttccgg gtggacggta gaagcatcca gcgggttatc accgtggtta atgcgcaaga
      781 agcccgcgca ctgctcgaag gctttcggcc ccagacggct gactttcagc agttgctgac
      841 ggttctggaa ctggccgttc tcatcgcgcc aggccacgat gttttgcgcc atcatgcgcg
      901 tcaggcccgc cacgcgggtt aacagcggaa cagaagcagt gttgagatcg acgccaacgg
      961 cgtttacgca gtcttctact actgcgtcca gtttgcgggc cagttgcgtc tggctgacgt
     1021 catgctgata ctgacctacg ccgatagatt tcggatcgat tttcaccagc tccgccagcg
     1081 gatcctgcaa acggcgggcg atagacaccg cgccacgcag cgaaacgtcg agatccggga
     1141 actcctgcgc tgccagctcg gaagctgagt aaaccgacgc gccagcttcg ctgacgatca
     1201 ctttctgtgc ggtcactttc gggaactgct tctgcacgtc gagatagaaa cgttcagttt
     1261 cgcgggaagc tgtaccgttg ccgatcgcca ccagttcaac gttatgtttt tcacacaagg
     1321 cagcaacggt catcgctgct tttgcggctt gtccggtgtg cgggtaaatg gtatcggtcg
     1381 ctaccagttt gccagtggca tcgaccaccg ccacttttac cccagtacgc agacccggat
     1441 cgaggcccat cgttgcacgc agtccggcag gggccgccat cagcagatcg tgcaggttac
     1501 gggcaaagac gttgattgct tcatcttccg cgcgttcgcg cacggtaccc atcagttcgg
     1561 tttccagatg catcagcacc ttgatgcgcc aggtccagct cactacgcct ttgcgccagc
     1621 tatccgccgg ggcattgttc aggcgcaggc caaggtgatc catgatgatt tgctcgcaat
     1681 agctctcttt gggcggctca tcgaactgcg gatcggcatt cagcgaaagc tggagtacgc
     1741 cttcgttacg cccacggaac atcgccagag cgcggtgaga aggcaccgtg gacaacggtt
     1801 cgtgatgatc gaaatagtcg cggaatttcg ccccttcctc ttctttaccg ctcaccaccg
     1861 tagaaaccaa atgcgcgttc ttccacagat aatcacgcac tttcgccagc agcgcggcat
     1921 cttcggcaaa ccgttccatc aggatatagc gcgcgccatc cagcgcggct ttggtatctg
     1981 ccacgccttt atcggcatca acatattgtg cagcggcgac ttctggcgtg tgtgacggat
     2041 cgctccacag caggtcagcc aacggctcaa gccctgcttc aatggcgatt tgcccgcggg
     2101 tgcggcgttt aggtttgtag ggcaggtaga ggtcttcgag ttcggttttg cttagggtgg
     2161 cgttgatggc cttcgccaga tcatcggtga gtttgccttg ctcggaaatg gacttgagga
     2221 tcgcctgacg tctctcttcc agctcgcgca gatagctcag acgcgtttcc agattacgca
     2281 gctgcgtgtc atccagaccg ccggtgattt ccttacgata acgtgtgata aacggcacgg
     2341 tattcccttc gtcaagcagg cgaacggcag cgtcaacctg ttccgggcgc gcctgaattt
     2401 cacccgcaat aatgcggcag aacgaatcat tcatcatggt ttgagttcat cttttcggat
     2461 caaaaatcag gggatagtta tacggactgg ctggcaaaaa tgccagccat cggcaggagg
     2521 ttaagactct tccttacggt ttcacgtact cgatagcatt aacataccag ctcgcttccc
     2581 cggcaggggt attcaccacc gccagatcgc cgacttcttt tttcagcaat gcgcgggcca
     2641 tcggggaatc gatagagatg taatctttac ggccaaaaat ttcatcgtag ccgacaatac
     2701 ggaaacggtg agtcacgcca tcgtcgtttt caatctccac ccacgcgcca aaaaagactt
     2761 tgccttcctg ctgaggggag taatcgacga ttttgagatt ttccatgcat ttagtgagat
     2821 agcgcacgcg acggtcgatt tcacgcagac gctttttatt atactgatag tcagcatttt
     2881 cgctgcggtc gcccagactt gcggcccagg tcaccttttt tgtgacctcc gggcgttctt
     2941 cacgccagag ataattaagc tcttgtttga gtttttcata cccttcccgg gtaaccaggg
     3001 gcgttttcat ctcgttgatt ccctttgtct gtttgataat gcgcacattg ggtataacgt
     3061 gatcatatca acagaatcaa taatgtttcg ccgaataaat tgtatactta agctgctgtt
     3121 taatatgctt tgtaacaatt taggctgaaa ttcataccag atttagctgg tgacgaacgt
     3181 gagctttttt aagaatacac gcttacaaat tgttgcgaac ctttgggagt acaaacaatg
     3241 caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa
     3301 cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga acagatggat
     3361 cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttaatgtt acctggtgaa
     3421 gatggcttgt cgatttgccg acgtcttcgt agtcagagca acccgatgcc gatcattatg
     3481 gtgacggcga aaggggaaga agtggaccgt atcgtaggcc tggagattgg cgctgacgac
     3541 tacattccaa aaccgtttaa cccgcgtgaa ctgctggccc gtattcgtgc ggtgctgcgt
     3601 cgtcaggcga acgaactgcc aggcgcaccg tcacaggaag aggcggtaat tgctttcggt
     3661 aagttcaaac ttaacctcgg tacgcgcgaa atgttccgcg aagatgagcc gatgccgctc
     3721 accagcggtg agtttgcggt actgaaggca ctggtcagcc atccgcgtga gccgctctcc
     3781 cgcgataagc tgatgaacct tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc
     3841 gacgtgcaga tttcgcgtct gcgccgcatg gtagaagaag atccagcgca tccgcgttac
     3901 attcagaccg tttggggcct gggctacgtc tttgtaccgg acggctctaa agcatgaggc
     3961 gattgcgctt ctcgccacga agttcatttg cccgtacgtt attgctcatc gtcaccttgc
     4021 tgttcgccag cctggtgacg acttatctgg tggtgctgaa cttcgcgatt ttgccgagcc
     4081 tccagcagtt taataaagtc ctcgcgtacg aagtgcgtat gttgatgacc gacaaactgc
     4141 aactggagga cggcacgcag ttggttgtgc ctcccgcttt ccgtcgggag atctaccgtg
     4201 agctggggat ctctctctac tccaacgagg ctgccgaaga ggcaggtctg cgttgggcgc
     4261 aacactatga attcttaagc catcagatgg cacagcaact gggcggcccg acggaagtgc
     4321 gcgttgaggt caacaaaagt tcgcctgtcg tctggctgaa aacctggctg tcgcccaata
     4381 tctgggtacg cgtgccgctg accgaaattc atcagggcga tttctctccg ctgttccgct
     4441 atacgctggc gattatgcta ttggcgatag gcggggcgtg gctgtttatt cgtatccaga
     4501 accgaccgtt ggtcgatctc gaacatgctg ctttgcaggt cggtaaaggg attattccac
     4561 cgccgctgcg tgaatatggc gcgtccgagg tacgttcggt tacccgcgcc tttaaccata
     4621 tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg ggggtaagtc
     4681 atgacttacg cacgccgctg acgcgtattc gcctggcgac cgagatgatg agcgaacagg
     4741 atggctacct ggccgaatcg atcaataaag atatcgaaga gtgcaacgcc atcattgagc
     4801 agtttatcga ctacctgcgc accgggcagg agatgccgat ggaaatggcg gatctcaacg
     4861 cagtactcgg tgaggttatt gctgccgaaa gtggctatga gcgggaaatt gaaaccgcgc
     4921 tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc gcggtggcga
     4981 atatggtggt caacgccgcc cgttacggca atggctggat caaagtcagc agcgggacgg
     5041 agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg ccggaacaac
     5101 gtaagcacct gttccagccg tttgtccgcg gcgacagtgc acgcaccatt agcggcacgg
     5161 gattagggct ggcaattgtg cagcgtatcg tggataacca taacgggatg ctggagcttg
     5221 gcaccagcga gcggggcggg ctttccattc gcgcctggct gccagtgccg gtaacgcggg
     5281 cgcagggcac gacaaaagaa gggtaaataa acgggaggcg aaggtgcctc ccgttttgct
     5341 ttctataaga tactggatag atattctcca gcttcaaatc attacagttt cggaccagcc
     5401 gctaccagcg cggcacccgc aggggtgtcg gtgtatttat cgaagttgtc gataaacagt
     5461 ttcgccaggg tttcggcttt ttcctgccac tgttccggag aagcgtaggt gttacgcgga
     5521 tcgagaatct tcgtgtctac gcccggcagt tcggttggga tcgccaggtt aaacatcggc
     5581 agagtgaagg tttctgcatt atccagcgaa ccgttgagga tggcgtcgat aatggcgcgg
     5641 gtatctttaa tcgagatacg tttgcctgtg ccgttccagc cagtgttaac cagataagcc
     5701 tgcgcgcccg ccgcctgcat acgtttcacc agcacttctg cgtactgagt cgggtgcagc
     5761 gacaggaatg ccgcgccgaa gcaagcggag aaggttggcg tcggttcggt gatgccacgc
     5821 tcagtaccgg ccagtttggc ggtgaagcca gagaggaagt gatactgggt ttgatcggca
     5881 gtcaggcgag aaaccggcgg caacacgccg aaagcatcag cagtcaggaa gataacctta
     5941 gtcgcgtggc ccgctttgga aaccggctta acgatgttat cgatgtgata gattggataa
     6001 gaaacgcggg tgttctcggt ttttgaacca tcatcaaagt cgatagtgcc atcttcacgc
     6061 acggtgacgt tttccagcag cgcatcacga cggatagcgt tgtagatttc aggttccgct
     6121 tctttcgaca gcttgatagt ttttgcgtag cagccgcctt cgaaattaaa cacgccgtca
     6181 tcgtcccagc cgtgttcgtc atcgccaatc aggcggcgtt tcgggtcggt ggaaagggtg
     6241 gttttaccgg tgccggaaag gccgaagaac accgcaacat cgcctttctc gccaacgttg
     6301 gcggagcagt gcatagaagc gatacctttc agcggcagca ggtagttcat catcgagaac
     6361 atccctttct tcatttcgcc gccgtaccag gtgccgccaa tcagctgcat gcgctcggtc
     6421 aggttaaacg ccacgaagtt ttcggagttc agaccctgtt ctttccactg cgggttagtg
     6481 cacttcgcgc cgttcataac gataaagtct ggtttgaaac ctgccagctc ttcatcgctc
     6541 gggcgaataa acatgttttt gacaaaatgc gcctgccagg ccacttcggt gatgaaacgg
     6601 acggaaagac gagtatccgg gttcgcacca cagaaagcgt cgacaacgaa cagacgtttg
     6661 ccggaaagct gcctggtcac caggcctttc agatgctgcc aggtttccgg agagagaggt
     6721 ttgttgtcgt tcttaccttt gcctttgtct gcccaccaga aagtatcgcg agtggtatcg
     6781 tcacggacga tatacttatc ttttggtgaa cgaccggtga agatcccggt atcgacggca
     6841 acggcaccca gattagttaa caccccgcgc tcataacctg tcaggctcgg atcgagctct
     6901 tcctgataca gcaggtcgta gcttgggttg taaacgatat catgtacgtc actgatacca
     6961 taagcctcga gttcttgcgg ggtcaaacca ttgttaacgc gcatttcact gctccttagc
     7021 caatatgtat tgcctgaata gtaaagtctt tttgggggtg ttaaccgcga caaggctcat
     7081 agatttacgt atctggagaa attcatattt gacaaaaaac cccgtgattc ctgttacgaa
     7141 acggttgcta ttatcgcaga aatagcattt taggtgtgga aaatgttctc aaaaggttaa
     7201 atcttggttt atatggcggg aatattgtga gtggaatcgc attccttaat gaaattgatg
     7261 caaattcaat cggtaaagta tcgattcacc acgctaactt tttttgatta ttttctgaaa
     7321 aaagcaaaat actactgccc ggatgcgata attaacccac cttttgattc tggataacca
     7381 acatggataa cgttgaactt tcaccggcaa cacgctgggg gatgattgct accggattac
     7441 ttcaggggct ggtttgctac ctgttgatcg cctggcttgc cggaaaaaat cacagttgga
     7501 ttgtttatgg cgtgcctgcg accgttgcgt tttcatccgt tttactcttc tccgtgatct
     7561 cttttaaaca gaagcgcctt tggggatggc tggcgctggt gtttattgcc acgacaggga
     7621 tgagcggttg gctgaagtgg caaactgatg gcatgaaccc ctggagagct gaaaaggcga
     7681 tttgggattt tggctgctat ctgctgttga tggcaatgtt gttgctaccg tggatacaac
     7741 aaagccttcg tatccgcaat tgcagtagcc gctatagcta cttttaccag tcagtatggc
     7801 ataacgtact tatattactg gtgatttttc tcgccaatgg cctgacgtgg ctggtgcttt
     7861 tactgtgggg tgagttgttt aaacttgttg gcatcaagtt tttcaatacg ctcttttttg
     7921 caaccgactg gtttatgtat cttacattag gtctggttac cgcactggcg gtgattctcg
     7981 cgcgcacaca gtcacgttta atcgactcta ttcaaaagtt gttcacgtta atcgccacgg
     8041 ggttgctgcc gttagtatca ttgctaaccc tgatgtttat catcactctg ccgtttacgg
     8101 gcctgagcgc gatttctcgc cacatctccg ccgccgggtt gctgttaacg ctggcctttt
     8161 tgcaattgat cttaatggct attgtccgcg atccgcaaaa agcgtcactt ccctggacgg
     8221 ggccgttgcg ttgcctgatt aaaaccgctt tgctggttgc tccgctgtat gtgttcgtcg
     8281 ccgcctgggc gttatggctg cgggtcgctc agtacggctg gactgtcgac cgcttgcagg
     8341 gcgcgctggc ggtgctggtg ttactggtgt ggtcgctggg gtattttgtc agtatcgtct
     8401 ggcgtaatgg gcaaaatccc cttgttcttc agggcaaagt gaaccttgcg gtttcgttat
     8461 tggtgttggt gatactggtg cttcttaatt cgccggtgct ggacagtatg cgtattagcg
     8521 tgaacagtca tatggcgcgt tatcagagcg gcaaaaacac gccagaccag gtaactatct
     8581 acatgctcga acagagcggt cgctatggac gtgcggcgct tgagtcgctg aaaagcgatg
     8641 ccgggtttat gaaagacccg aaacgcgcgc gggatctgct gatggcgtta gatggagagc
     8701 aacatcttca ggaacaggta tcggaaaaag tattagccga aaatgtgtta attgcccctg
     8761 gttctgttaa accggatgtg acattctggt cggccttaat ccaggatcgc tataacgtga
     8821 tgacctgtat tgaaaaagac gcctgcgtcc tggtcgagca agatctgaat agtgatggtc
     8881 aggcggagcg gatcctgttt gcttttaatg atgacagagt cattgtctat ggctttgact
     8941 cagacagaaa agaatgggac gcgcttgata tgagtttact tccgaacgaa ataacgaaag
     9001 aaaaattact cacagctgcg aaggatggaa aactggggac gaggccgaaa gcgtggcgcg
     9061 atctgacggt agatggtgaa acgctggaaa taaacttaag taagtgacaa cgcttttatc
     9121 ggatacgacg ctgtatctga ccaaaataat ttgcgattta tttcataggc gctggtaagc
     9181 gcagcacatc aggccattaa gttgtaaaat ccggcagagc cctctctgcc ggacatactc
     9241 attaatgaac ttgcggatct gccggagacg cgttgttgcg gatttcagca atatccatcg
     9301 cattgaacag atagtggtta ccgcagtaat cacaatgcat gtcaatttcg ccatcttccg
     9361 ccaggatgct atcaacttct tcatcaggca gcgttttcag cgcatcggcg caatgttcac
     9421 gcgagcaggt gcatttgaac tccacatcct gcggatcgta aaccgtcacc tcttcttcgt
     9481 gatacaaacg ccacaacact tcgtttgccg gtaaggtcag cagttcttcg gttttgatgg
     9541 tttcggttag cgtcgccagg tggtcaaagt cgtcctgctg ggcattttgc gcaggcatta
     9601 cctgcaacaa cataccgcct gcagccggtt tgccgtctac gtcgccggtg cgaataaaca
     9661 ggcgcgtcgg cagctgttca gaacgcataa agtaatcttc caggcaggcc gccagggtat
     9721 caccttccag accaactacg ccctgatagc gttcgccttc gctcggggta atggtgatca
     9781 ccacgtaacc attgccgacc agcgttttca ggtcggcatt ttctggaatt tcgccctgca
     9841 cgcgcgccac accgcgcatt tgctggttat tgttaccgtt aataaccgcc agattcatcg
     9901 gaccgtcgcc ctgcagctgt acggtgatat caccatcaaa cttcagcgta gcggttaaca
     9961 ggctggtcgc aaccagcagt tctgccagca cgtttttaac gggctgcgga taatcgtggt
    10021 tctcaaggat ctgttgcagg gtttccgaaa cggttaccag ttcgccgcga acggcaaaat
    10081 tttcaaacag atagcgatgt aattggtcat gttgcggcat aatcatctct cttgcaggtg
    10141 acagttattc actgtcgccg tgtttaaatc gtaacaggtc gcggcgctct tttttgtccg
    10201 ggcgtcggtc cgggtgcggc atggttaagg cattaagttt acgtgccatc gccatttttt
    10261 cgcgtttctc tacactttcc gcagtctctt catacagcaa ggctgcctcg ctggcggggc
    10321 gacgctgttc agtaatcgcc tttacaatca ccgtgcgttc gtcatttccc tggcgcagag
    10381 tgagcgtggc attcagctcg acgattttgc tcggcttgct gcgctgcccg ttgtaatgca
    10441 ccttaccgcc ttcaatcatt tcacgggcta gcgcgcgggt tttataaaaa cgggcagccc
    10501 atagccattt atccagtcga acctcaacag caggtttctc tttcatggcg tctccttcac
    10561 attagcgagg ggatcaggcg gcggtagtca ttcagtgacg gatggcgttg atactgtttc
    10621 tcggcaatcc cggaatcagg attagtcacg ccgaggcagt aacgaatacc aaattgcgcg
    10681 gcagcatcga gaatcgcttc gctgtcatca ataaacagcg ttctttcagc tttcagaccc
    10741 gtagcttcgg ccaccgcatg ccataaccgc tgatcctctt tcggataacc aaatgtgtgg
    10801 gtggaaagta ataaatcaag gtgtgcgtcc agacccgtat gctcaagttt taccgccagg
    10861 ttgtgcggat gagcattggt gagcaaaatt cgctgcttac cgctggcttt cagtgcctca
    10921 agaaacggaa tggtatcttc acgcagtacg gcgcgcggtc ccatctcggt ggtcattgca
    10981 cagatatcca gacccagttg ctcactccag taatcaagac agtaccagtt tagcgtatgc
    11041 tgtacgtcgt gatattgctg gcgcatatat tccatcgctt cctgtggcgt aaccccgttt
    11101 ttcgcgcccc atgtttcagg caccagcttt tgccagaaat agttatcgaa ggcgaggtcg
    11161 agcaacgtgc cgtccatatc cagcagaacg gtatctacgt cctgccaggc aatgttgata
    11221 tgcatgaggg aaatctccag agtgaagcaa tttgcgcgac agggtagcat aacctgtcgc
    11281 gcaaacgtgt tattcgataa ggctttctga aggggtgatc agttgcgggt tcaggcagct
    11341 ttcataatag gcctgaatct ccatcatgcg agtgcgatga cgctggtaac gacgccaggc
    11401 ctgtacgcca ttgtaaatgg cgctacccag catcgtcagt agcagcaatg tggtgctgag
    11461 atagcgccac aggccggaac gatccgggat cggatgaagg ccaatatgct gcgtaccatt
    11521 ggcgtcagtg aagatttttg tgacgatacc ttcggcgtta aacggcgtat gcatcagcat
    11581 ttgtgccagt ttctggaaag cgttccactg ttcttgcggc gggtagtcgt aaagtgatgc
    11641 cgaaggccag ggctgatcaa caaaatcgct gccttcgtcg ctgacaatca ggaatccgcc
    11701 gggggccgga ctgtttagtg attgtgccgc tcgcgccgtt tcatgcgtga taaacggcgc
    11761 ggtggaggtt gccaccaggt tatccagcga ttccgcactc accgggcgta ataacacatt
    11821 cacgccatcg agcttcccgg cgttggcgcg ttttaccagc gcgtcccagt ctttactgtt
    11881 gccgagattg accagcgcat ttttcagtcg cacacagtca tctttggcag aacataaatc
    11941 cgctgtcttc agtacaatgt cgccaaaatc atcaagcaat accatgccgg atttttgaat
    12001 cgctgaacgt aatgaggcac tgacgcgaga ttcatcttcc ggtttagggt gcagctggcg
    12061 attaactgct tcagtcaatg ccgtcgcttt gttgaccagt tcagattctg gtaatggtaa
    12121 tgagcgggcg tcattccaga tgatctgcga gcagtcaaac ggtaaaaaag gtgaattggt
    12181 tttcgcgctc caggttccgg aagtgcgaat attacacatt cccgtaccgc taatacgcaa
    12241 tgtatcgcct acccgcacgc cagcgtcagc cagttgtttt acgctggtgg cttcaatggt
    12301 ctgcgcgcct ttcatccatg agagagtgaa tttcagcggc atatccagcg ggatccagaa
    12361 taacagcata aacagcacca gcagcgagcc cgcagcgata atcgtactgc gcagccagtg
    12421 ctgtaacgga aagtttttta cttcatcatg cagcgaaaga tatcgcccct gacgcactac
    12481 gtggcagtcg agatagatat caatatcggt ttgttgatcg agatcctgag caatgtatgg
    12541 ctgccagtgt gcgggataga ccaggtcgat aataccgagt gaaatattgt tgatctgttc
    12601 ctgatcgttt tcgccaaaca atccccaacg acggggtgta ccgcgcagac aatgaatttc
    12661 ccgcagggag gattttgccg ggggcgcgaa tagcccccac agacctgcgc ccagcagcag
    12721 taatgcgccg cctgccagcc acggaacaaa tacatccggg gtaatcaggc agaaaaagaa
    12781 catcaggaag gaggcgacga tcagcaacgc ttcacgcagc ccgcgcggac gactcagcgc
    12841 atattcttca tgcgtttctt tgcgaatatt gagtagctcg atttgctcac tctcttcccc
    12901 gcgaatcgac gcctgcgtgg acggaacagg ttgcaaggca tagccgcgcg tttcctgcat
    12961 gtactcctgt agcgtatgac cgttgagtga aataaccaac ggcagcgaat cggtatgaat
    13021 cagttcaacg gtattctcat cgttgatgta ctgttcccag aaggggggca ggtggacttc
    13081 taccgaatcg aggtagtaac gccatttatt cggatcgtcg gtagagatgc cgtaacgtgt
    13141 gatagcgtgt gtcagcatca tgacgttgtt gctttcggca ttcagcgcca gagagatcgg
    13201 tgccgcgcta gctcccgttg ggccaggcac ctgtaatacc tgcgtcaggc tctcaagata
    13261 attttcaacg gcgctacgtt cttcaggtgt gagtttacgc gtttgcgcat ccgcgaaggc
    13321 gttggtccag ggcagctgac tccgtctgga tcgcactttt atcagccatc ccgcaagtag
    13381 tgagcaggcc agcaaagcag ctaaaaaaat cacaatggtg ctcatgcttt ccccatctta
    13441 cttagtctgt caggcgtggt cagtcgcacc agacacaata catgattctg cggttggcta
    13501 tcggcaagcg tggagtttaa cttgagcatc attaagcaca tcacagtaac cgctgatcat
    13561 agcaaaccct ctgaaggcgg aatatcagca aattcctaga gttatttcaa cctattgact
    13621 tttaatttga tttgaggacg attatttatt aagttacata aatgtaaata acatgcaatt
    13681 cacattgccg aaagcgtgtc cgatatcgca caataaccgc tgatttcaca gcatagatcc
    13741 cacgatgagc aaatcattac aaaaacccac cattctgaat gttgaaactg tagcccgttc
    13801 ccgactgttt accgtcgaga gcgtggatct ggagttcagc aatggcgtgc ggcgtgttta
    13861 tgaacgaatg cgtccaacca accgggaagc agtgatgatt gtgccgattg tggacgatca
    13921 cctgatcctg atccgcgaat acgcagtggg aactgaatcc tacgaattag gtttttcgaa
    13981 aggattaatt gatccgggtg aaagcgtcta cgaagccgct aaccgcgagc taaaagaaga
    14041 ggttggattt ggtgcgaacg atctgacttt tttgaagaag ctcagcatgg caccgtctta
    14101 cttttccagc aaaatgaata tcgtggtcgc gcaagatctc tacccggaat cgctggaagg
    14161 cgatgagcca gagccgctac cacaggtgcg ctggccgctg gcgcatatga tggatttgct
    14221 ggaagaccct gacttcaacg aagcgcgtaa tgtcagcgcg ctgttcctcg tgcgcgaatg
    14281 gttgaaaggg caggggcgag tgtaaatgcg tgaagtttgc ctgatgcaca acgcttatca
    14341 ggcttatggg gttcctgcaa tatattgatt tttcacgatt ttgttagccg ggtaacgcgg
    14401 tcacccgcat ccagcataaa caaatgcact ttgtcagcca actgaaaagg cgccgaagcg
    14461 cctttttaat cagaacaatt cctgtgcctc accattatcg ataatggtcg ttcccacctc
    14521 gtgcaccgcc tgttgtgtcg gctgcgtacc ttcgatgaaa tactcttcgc ggctgttgcc
    14581 accattagct aactgcccgg tgctgcgatc gatattcacc gtcacaatac ccggtggcgg
    14641 cgtcagcggc tgctccggca caccttcaag aacggctttc atataagcgt cccatgcagg
    14701 ctgggcactc ttggctccgc cttcgtaacc tgagatctga tctttaatcg ctccggaagc
    14761 cgttgtatga ccgagattac gacggtgatc atcaaagcca atccagaccg aggtcacaac
    14821 gcccggaccg taacccgaga accacgcatc tttcgaactg ttagtggtac cggttttccc
    14881 gccgatatcg cgacgctgca aatcacgacc tgcacgccag ccagtaccct gccagcctgg
    14941 ctcaccaaag atattggtgt tcaaagcact cttaatcagg aatgccagcg gagtgttgat
    15001 gacgtgcggt gcgtactcct gcgcgccagt cttcgccact aacgcctgat ttgcctgctc
    15061 cagctgcggc attggtacag aaacattctg ctgctcgcgg gagatagcga catcttcaac
    15121 atcgttattt tccagcacgt tcgatttctg cgtatcaccg taaatcaccg gaatatcgca
    15181 ttccgggcag gctactttcg gtttcgcttc gaaaatcacg ccgccctgat cgttttcaat
    15241 tttgctgata aaccacgggt ccaccaggaa gccgccgttc gccatgaccg cgtagccgcg
    15301 cgccacctgc attggggtga aggacgctga acccagcgcc agcgattcgg tgtggacaat
    15361 gttttgcgcc gggaagccga agcgttgcag atattctgca gcgtagtcga cgcccatagc
    15421 ccgcattgca cgtaccatca ccacgttttt cgactgaccc agcccctgac gtaagcgaat
    15481 tggaccagca tattgtggcg gcgagttttt cggctgccag tcagaaccgg cacctgcgtc
    15541 ccagcgagaa attggcacat cgttcaacat acttgccagc gtcagacctt tatccatcgc
    15601 cgcggtgtag aggaacggtt tgatgttgga acccacctga cgcagtgcct gggtggcgcg
    15661 gttaaacttg ctctgattga aatcaaagcc accgaccagc gccataacgg caccgttttg
    15721 cggattgatc gacaccagcg ccgagttcac ttccggcact tgtgccagcc accatgcatc
    15781 gccaacctga cgaacccaga tttgctgacc cgtttgcaga acatcggtca ctttacgcgg
    15841 cgtcggtccc tgctgagtat ccgaacggta aggacgcgcc cagcgaacgc cttccatact
    15901 caatgcgacg gtcgacccgt ccgccagcat cgccgtcgct ccctgaggat tggcgctggt
    15961 gactgcggca ggcagcagcg gaccataggt cggcagcgcc ttcagcgtat cggtaatctt
    16021 gttgttatcc cacgccgact cgcccacttt ccacagcaca tttgccgggc cgcgatagcc
    16081 gtggcgcatg tcgtagtcca gcacgttatt acgtaccgcc tgctgtgcgg cctgctgcac
    16141 tttgcgggtg atggtggtgt aaatgcgata accgtcttca taggcacttt cgccataacg
    16201 gttatacatc tcctggcgca ccatttcgct caggtacggc gcagagaaag caatctccgg
    16261 cgcgtgatag ttagcgttaa tcgcctcagt gcgtgtctga tcgaactgtt gttgggtgat
    16321 atacccttca tccagcatcc gcgacagtac gacgttacgc cgcgcgacgg cacgatccat
    16381 tgagtagagc ggattaaagg tggaaggcgc tttcggcagc ccggctatca cagccatttc
    16441 gttcagcgtc agttggtcga ccgttttacc gaaatagact tgtgccgcag caccgacacc
    16501 ataggcgcgg taaccgaggt aaatcttatt cagataaagc tcgaggatct cgtctttcgt
    16561 cagcaactgt tcaatgcgaa tcgcgaggaa gacttcctta atcttacgca tcagcgtgcg
    16621 ttctggactg aggaagaagt ttctcgccag ctgctgggta atggtactcg ccccttgtga
    16681 cgcgtggccg gagaacagcg ccacacttgc cgcacggaag atccccaccg ggtcaacgcc
    16741 gtgatgctcg tagaagcggc tgtcttccgt cgcgataaag gctttcacca tctccggcgg
    16801 gatttgatcc aacgtaaccg gaatacgacg tttctcaccg tattgagcaa tcagctcgcc
    16861 atcggcgctg taaatctgca tcggaatttg caggcgaaca tcttttaatg tcgccacatc
    16921 cggcagttgt ggctcgatgt agcggtatag gccataaatc gagcctgctc ccagcagaat
    16981 gcaacagact gcaaggatca aaaaatactt tacgaacttc actggaaatt tcccatttag
    17041 tttcatttgg gcagtttata aacaaacgcg cgttagtata aaggcaagcc agacgcattg
    17101 atatacccgt cagagtgacg ggtgataagg agatcatcac aatggcattt aagatctggc
    17161 aaattggttt gcatttacaa cagcaagaag cggtagcggt tgcgatcgtg cggggcgcaa
    17221 aagaatgctt tttgcaacgc tggtggcggt tgccgctgga gaacgacatt atcaaagatg
    17281 ggcggattgt tgatgcgcag cagctggcta aaacgttgtt accttggagt cgcgaactgc
    17341 cgcagcgtca tcacattatg ttggcgtttc ccgccagtcg cacattacag cggtcatttc
    17401 cgcgcccgtc gatgtccctt ggtgagcggg agcaaacggc ctggctgtca gggacgatgg
    17461 cccgcgagct ggatatggat ccggactccc tgcgcttcga ttacagcgaa gactcactca
    17521 gtcccgccta taacgtgact gccgcgcaaa gcaaagagct ggcaacgctg cttactctgg
    17581 cagaaaggtt gcgtgttcat gttagtgcga tcaccccgga tgccagtgca ttacagcgat
    17641 tcctgccttt tttaccttct catcagcaat gtctggcctg gcgtgataac gaacagtggc
    17701 tgtgggcgac gcgctatagc tgggggcgca aactggcggt ggggatgact agcgcgaagg
    17761 agctggcggc agcgttattc gttgatcccg acagcgtcgc gatatgtggc gaaggcggat
    17821 ttgacccctg ggaggccgtt tctgttcgtc agccgctgct accgccgcct ggtggagact
    17881 ttgccatcgc gctggggctg gcgcttggga aggcgtactg atgaacccgc caattaattt
    17941 tttgccctgg cgacagcaac gccggaccgc ttttctgcgt ttctggttgc tgatgttcgt
    18001 tgcgcctctg ctgctggccg tcgggataac gctaatactg cgtctgacag gcagcgccga
    18061 agcgcgcata gacgcagttt tgcttcaggc ggaacaacaa ctcgcccgca gcttacagat
    18121 aacgaagcca cgtttgctgg agcaacagca gttacgcaaa cagcgttcgc aacggcagcg
    18181 ccagcgacaa tttacccgcg actggcaatc tgcgctggaa gcactggcgg cgcttttacc
    18241 cgagcacgcc tggctgacaa cgataagctg gcagcaggga acgctggaga tcaaggggct
    18301 gacaacaagc attaccgcgt taaacgcact agaaacgtca ctccgccagg atgcgtcttt
    18361 tcatctcaat cagcggggag ccacgcagca ggatgcgcag ggacgctggc aatttgagta
    18421 tcagttaaca aggaaggtta gcgatgaaca tgttctttga ctggtggttc gccacatcac
    18481 cccgcctccg ccagttttgc tgggcattct ggttgctgat gttagttacg ctcatttttc
    18541 tgtcatcgac acaccatgaa gagcgcgacg cattaattcg attacgggca agtcatcacc
    18601 agcagtgggc cacactgtat cgcctggttg acaccactcc cttcagcgaa gaaaaaacgc
    18661 tgcccttttc gccactggat tttcagttgc ccggcgcgca actggtttcc tggcatccat
    18721 ccgcgcaggg aggtgagttg gcgttgaaaa cgctgtggga agcagtgccg tcggcattca
    18781 cacggctggc agagcgcaac gtcagcgtga gccgtttttc gttaagcgtg gaaggtgatg
    18841 atcttttgtt cacgctacaa ctggagacgc cgcatgaggg ttaaacgctg gttgttggca
    18901 ggtattgcat tgtgcctttt aaccggtatg cgtgaccctt ttaaaccgcc ggaagatcta
    18961 tgccggatta gcgaacttag ccagtggcgc tatcagggga tggtagggcg aggcgagcgc
    19021 atcatcggtg taataaaaga cgggcaaaag aaatggcgac gggtgcagca aaacgatgtg
    19081 ctggaaaacg gctggacaat tttacagctg acgccagacg tactaacgct gggtaccggg
    19141 acaaactgcg aaccgccaca atggttgtgg caacggcaag gagatacaaa tgaagcaatg
    19201 gatagccgca ctactgttga tgctgatacc cggcgtacag gcggcaaagc cgcaaaaagt
    19261 gacgctgatg gtggatgacg ttccggtagc tcaggtgttg caggcgctgg ctgaacagga
    19321 gaagttgaac ctggtcgtgt cgccagacgt cagcggtacg gtgtcgttac atctaacaga
    19381 tgttccctgg aagcaggcac tacaaactgt agtgaaaagc gccggactga taacgcggca
    19441 ggaaggcaac attctctcag tgcattccat tgcctggcag aataacaata tcgcccgcca
    19501 ggaggcggag caggcgcggg cgcaggcaaa tctgccgctg gaaaatcgca gtataaccct
    19561 gcaatacgcc gacgcgggag aactggcgaa agcgggggag aagctactga gtgccaaagg
    19621 gagtatgacc gtcgataaac gcaccaatcg ccttttgcta cgagataaca aaacggcgtt
    19681 aagcgcgctt gaacagtggg tagcgcaaat ggatctgccg gtcgggcagg ttgagctgtc
    19741 ggcgcatatt gtcaccatta atgaaaaaag tttgcgtgag ttaggcgtga aatggacgct
    19801 ggccgatgcg caacacgctg gtggcgttgg gcaagtcacc acgcttggta gcgacctctc
    19861 cgtagcgacg gcgacaacgc atgtcggttt taacattggg cgcatcaacg gacgcttgct
    19921 ggatcttgag ctttccgcgc tcgaacaaaa acagcagctg gatattatcg ccagtccgcg
    19981 tctgctggcc tcacatcttc agcctgccag cattaaacag gggagcgaaa ttccatatca
    20041 ggtttccagc ggggaaagtg gcgcgacgtc ggtggaattt aaagaggccg tcctggggat
    20101 agaggtcacg cccacggtgt tacaaaaagg tcgcatccgg ctgaaattac acatcagcca
    20161 gaacgttccg gggcaggtgc tacagcaggc cgatggcgaa gtgctggcga ttgataagca
    20221 ggagatcgaa acgcaggtcg aggtcaaaag cggagaaacg ttggcgctgg gcggcatttt
    20281 tacccgtaaa aataaatcgg gtcaggatag cgtaccgttg cttggcgaca ttccctggtt
    20341 cgggcaatta tttcgtcatg acggaaaaga atatgaacga cgcgagttag tggtgtttat
    20401 cacgccacga ctggtttcca gtgagtaaac agccgtaaaa gcggtaatgt ttttatgctg
    20461 aacgtgtttc atctatttga cgcgcgcagg tatttagcat acaaggagta ccgatttgag
    20521 agttggcgct cttcgctgcc tgcgttccat gatgatgatt tatcattcag gcggcatttt
    20581 gctgtctttt ttacgctaat cttacccggt gatttatcgc cagagcggtg gtagcaaggc
    20641 agcgcgcttg cagcgaccag atatgcagag ggatgggtga tttattcagt tgccaaaccc
    20701 gctggagtat tgagataatt ttcagtctga ctctcgcaat atcttatgag gtttcagttc
    20761 atgtcctgcg gcgctctctg agcgaagcgg gtttatcatt aacgaatagt cttagtagta
    20821 ccgaaaaaat ggcagagaaa cgcaatatct ttctggttgg gcctatgggt gccggaaaaa
    20881 gcactattgg gcgccagtta gctcaacaac tcaatatgga attttacgat tccgatcaag
    20941 agattgagaa acgaaccgga gctgatgtgg gctgggtttt cgatttagaa ggcgaagaag
    21001 gcttccgcga tcgcgaagaa aaggtcatca atgagttgac cgagaaacag ggtattgtgc
    21061 tggctactgg cggcggctct gtgaaatccc gtgaaacgcg taaccgtctt tccgctcgtg
    21121 gcgttgtcgt ttatcttgaa acgaccatcg aaaagcaact tgcacgcacg cagcgtgata
    21181 aaaaacgccc gttgctgcac gttgaaacac cgccgcgtga agttctggaa gcgttggcca
    21241 atgaacgcaa tccgctgtat gaagagattg ccgacgtgac cattcgtact gatgatcaaa
    21301 gcgctaaagt ggttgcaaac cagattattc acatgctgga aagcaactaa ttctggcttt
    21361 atatacactc gtctgcgggt acagtaatta aggtggatgt cgcgttatgg agaggattgt
    21421 cgttactctc ggggaacgta gttacccaat taccatcgca tctggtttgt ttaatgaacc
    21481 agcttcattc ttaccgctga aatcgggcga gcaggtcatg ttggtcacca acgaaaccct
    21541 ggctcctctg tatctcgata aggtccgcgg cgtacttgaa caggcgggtg ttaacgtcga
    21601 tagcgttatc ctccctgacg gcgagcagta taaaagcctg gctgtactcg ataccgtctt
    21661 tacggcgttg ttacaaaagc cgcatggtcg cgatactacg ctggtggcgc ttggcggcgg
    21721 cgtagtgggc gatctgaccg gcttcgcggc ggcgagttat cagcgcggtg ttcgtttcat
    21781 tcaagtcccg acgacgttac tgtcgcaggt cgattcctcc gttggcggca aaactgcggt
    21841 caaccatccc ctcggtaaaa acatgattgg cgcgttctac cagcctgctt cagtggtggt
    21901 ggatctcgac tgtctgaaaa cgcttccccc gcgtgagtta gcgtcggggc tggcagaagt
    21961 catcaaatac ggcattattc ttgacggtgc gttttttaac tggctggaag agaatctgga
    22021 tgcgttgttg cgtctggacg gtccggcaat ggcgtactgt attcgccgtt gttgtgaact
    22081 gaaggcagaa gttgtcgccg ccgacgagcg cgaaaccggg ttacgtgctt tactgaatct
    22141 gggacacacc tttggtcatg ccattgaagc tgaaatgggg tatggcaatt ggttacatgg
    22201 tgaagcggtc gctgcgggta tggtgatggc ggcgcggacg tcggaacgtc tcgggcagtt
    22261 tagttctgcc gaaacgcagc gtattataac cctgctcaag cgggctgggt taccggtcaa
    22321 tgggccgcgc gaaatgtccg cgcaggcgta tttaccgcat atgctgcgtg acaagaaagt
    22381 ccttgcggga gagatacgct taattcttcc gttggcaatt ggtaagagtg aagttcgcag
    22441 cggcgtttcg cacgagcttg ttcttaacgc cattgccgat tgtcaatcag cgtaacaaca
    22501 agaaaggtca ggccgcttat caagcggtct attagcttca ggttaattgc aacgtggtaa
    22561 gcattaacct tttagtgggg tgttaaatgg atgaattcaa accagaagac gagctgaaac
    22621 ccgatcccag cgatcgtcgt actggtcgtt ctcgtcaatc ttctgaacgt tctgagcgta
    22681 ctgaacgtgg cgaaccgcag atcaattttg atgatattga acttgatgac actgacgatc
    22741 gccgtccgac tcgtgcgcaa aaagagcgca atgaggaacc ggaaatcgaa gaagaaattg
    22801 acgaatccga agatgaaacc gtggatgaag agcgcgtaga gcgtcgtccg cgtaagcgca
    22861 aaaaagcagc cagtaaaccc gcttctcgtc agtatatgat gatgggcgtc ggcattctgg
    22921 ttctactgct gttgatcatc ggtatcggtt ctgcgctaaa agccccctcg acctcttcca
    22981 gcgatcaaac cgcgtctggc gataagagta ttgatcttgc aggcaatgcg accgatcagg
    23041 cgaatggggt gcagccagcg ccgggaacca cgtctgcgga aaatactcag caggatgttt
    23101 ctctgccacc gatctcttct acgccgactc aagggcaaac cccggcggca acggatggtc
    23161 aacaacgtgt tgaagtgcag ggtgacctga acaatgcgct gactcagcca caaaatcagc
    23221 aacagttgaa caatgtggcg gtcaattcca cattgccgac cgaacccgcg actgtcgcgc
    23281 ctgttcgcaa tggcaatgca tcgcgtgaca cggcgaaaac gcaaaccgct gaacgtccgt
    23341 ccactacgcg cccagctcgt cagcaggcgg tgattgaacc gaaaaaaccg caagcaaccg
    23401 tgaaaacgga gccgaagccg gtagcacaga cgccgaagcg tactgaacca gctgcccctg
    23461 tggcgagcac gaaggcaccg gctgcgactt ctacgccagc accaaaagag acggcgacta
    23521 cggctccagt acagacggca tccccggcgc aaaccacggc aacaccagcc gctggaggga
    23581 agaccgcagg taatgttggt tcgttgaaat cggcaccgtc cagccattac actctgcagc
    23641 tgagcagttc ctctaactac gacaacctga acggttgggc gaagaaagag aatctgaaaa
    23701 actacgttgt ctatgaaacg acgcgtaatg gtcagccgtg gtatgtcctg gtttctggcg
    23761 tgtacgcttc gaaagaagag gcgaaaaaag cggtatctac attgccagca gatgttcagg
    23821 ccaaaaaccc gtgggcgaaa ccgctgcgtc aggtacaggc cgatctgaag taatcaaggt
    23881 tatctcccgc aatggtttat cgttgcggga gctgcctgaa gcgctggatg ctgtcggagc
    23941 tttctccaca gccggagaag gtgtaattag ttagtcagca tgaagaaaaa tcgcgctttt
    24001 ttgaagtggg cagggggcaa gtatcccctg cttgatgata ttaaacggca tttgcccaag
    24061 ggcgaatgtc tggttgagcc ttttgtaggt gccgggtcgg tgtttctcaa caccgacttt
    24121 tctcgttata tccttgccga tatcaatagc gacctgatca gtctctataa cattgtgaag
    24181 atgcgtactg atgagtacgt acaggccgca cgcgagctgt ttgttcccga aacaaattgc
    24241 gccgaggttt actatcagtt ccgcgaagag ttcaacaaaa gccaggatcc gttccgtcgg
    24301 gcggtactgt ttttatattt gaaccgctac ggttacaacg gcctgtgtcg ttacaatctg
    24361 cgcggtgagt ttaacgtgcc gtttggccgc tacaaaaaac cctatttccc ggaagcagag
    24421 ttgtatcact tcgctgaaaa agcgcagaat gcctttttct attgtgagtc ttacgccgat
    24481 agcatggcgc gcgcagatga tgcatccgtc gtctattgcg atccgcctta tgcaccgctg
    24541 tctgcgaccg ccaactttac ggcgtatcac acaaacagtt ttacgcttga acaacaagcg
    24601 catctggcgg agatcgccga aggtctggtt gagcgccata ttccagtgct gatctccaat
    24661 cacgatacga tgttaacgcg tgagtggtat cagcgcgcaa aattgcatgt cgtcaaagtt
    24721 cgacgcagta taagcagcaa cggcggcaca cgtaaaaagg tggacgaact gctggctttg
    24781 tacaaaccag gagtcgtttc acccgcgaaa aaataattct caaggagaag cggatgaaac
    24841 agtatttgat tgccccctca attctgtcgg ctgattttgc ccgcctgggt gaagataccg
    24901 caaaagccct ggcagctggc gctgatgtcg tgcattttga cgtcatggat aaccactatg
    24961 ttcccaatct gacgattggg ccaatggtgc tgaaatcctt gcgtaactat ggcattaccg
    25021 cccctatcga tgtacatctg atggtgaaac ccgtcgatcg cattgtgcct gatttcgctg
    25081 ccgctggtgc cagcatcatt acctttcatc cagaagcctc cgagcatgtt gaccgcacgc
    25141 tgcaactgat taaagaaaat ggctgtaaag cgggtctggt atttaacccg gcgacacctc
    25201 tgagctatct ggattacgtg atggataagc tggatgtgat cctgctgatg tctgttaacc
    25261 caggttttgg cggtcagtcg ttcattccac aaacactgga taaactgcgc gaagtacgtc
    25321 gtcgtatcga cgagtctggt tttgacattc gactggaagt ggacggtggc gtgaaggtga
    25381 acaacattgg cgaaatcgct gcggcgggcg cggatatgtt cgtcgccggt tcggcaatct
    25441 tcgaccagcc agactacaaa aaagtcattg atgaaatgcg cagtgaactg gcaaaggtaa
    25501 gtcatgaata agtttgaaga tattcgcggc gtcgcttttg atctcgacgg tacgctggtt
    25561 gacagtgctc ccggtcttgc tgctgcggta gatatggcgc tgtatgcgct ggagttaccc
    25621 gtcgcaggtg aagaacgcgt tattacctgg attggtaacg gcgcagatgt tctgatggag
    25681 cgtgcattgg cctgggcgcg tcaggaacgt gcgactctgc gtaaaacaat gggtaaaccg
    25741 cccgttgatg acgacattcc ggctgaagaa caggtacgta ttctgcgtaa actgttcgat
    25801 cgctactata gcgaggttgc cgaagagggg acgtttttgt tcccgcacgt tgccgatacg
    25861 ttgggcgcgt tgcaggctaa aggcctgccg ctaggcctgg tcaccaacaa accgacgccg
    25921 ttcgtcgcgc cactgctcga agccttagat attgccaaat actttagcgt ggttatcggc
    25981 ggcgatgatg tgcaaaacaa aaaaccgcat ccggacccgc tgttactggt ggctgagcgg
    26041 atgggaattg ccccacaaca gatgctgttt gtcggcgact cacgcaatga tattcaggcg
    26101 gcaaaagcgg caggttgccc atcagttggc ttaacctacg gatataacta cggcgaggct
    26161 atcgatctca gccagcctga tgtaatttat cagtctataa atgaccttct gcccgcatta
    26221 gggcttccgc atagcgaaaa tcaggaatcg aaaaatgact aagcccatcg tttttagtgg
    26281 cgcacagccc tcaggtgaat tgaccattgg taactacatg ggtgcgctgc gtcagtgggt
    26341 aaacatgcag gatgactacc attgcattta ctgtatcgtt gaccaacacg cgatcaccgt
    26401 gcgccaggat gcacagaagc tgcgtaaagc gacgctggat acgctggcct tgtatctggc
    26461 ttgtggtatc gatcctgaga aaagcaccat ttttgttcag tcccacgtac cagaacatgc
    26521 gcagttaggc tgggcactga actgctatac ctacttcggc gaactgagcc gcatgaccca
    26581 gtttaaagat aaatctgcgc gttatgccga gaacatcaac gctggtctgt ttgactatcc
    26641 ggtgctgatg gctgcggaca tcctgctgta tcaaactaat ctggtaccgg tgggtgaaga
    26701 ccagaaacag cacctggaac tgagtcgcga tatcgcccag cgtttcaacg cgctgtatgg
    26761 cgagatcttt aaggtgccgg agccgtttat tccgaaatct ggcgcgcgcg taatgtcgct
    26821 gctggagccg accaagaaga tgtccaagtc tgacgataac cgcaataacg ttatcggcct
    26881 gctggaagat ccgaaatcgg tagtgaagaa aatcaaacgc gcggtcactg actccgacga
    26941 gccgccggta gttcgctacg atgtgcagaa caaagcgggc gtttccaacc tgctggatat
    27001 cctttctgcg gtaacgggcc agagcatccc agaactggaa aaacagttcg aaggcaagat
    27061 gtatggccat ctgaaaggtg aagtggctga tgccgtttcc ggtatgctga ctgaattgca
    27121 ggaacgctat caccgtttcc gcaacgatga agccttcctg caacaggtga tgaaagacgg
    27181 cgcagaaaaa gccagtgtac acgcttcccg tacgctgaaa gcggtgtacg aagcgattgg
    27241 ttttgtggcg aagccgtaat atcaaaacgc tcggacggtc ccctcgcccc tttggggaga
    27301 gggttagggt gaggggagca ggcaagcgcc agtgggaatt accctttgct cgcattgtcg
    27361 aataaatcac ttcgtcatca ctctgaaacc ggggaaaccc ggttttttta tcctcatttg
    27421 caataatccg aaaaaatgtg aagcgcctcg ccgtttccac atctcacagt tgccacttat
    27481 cttcatttta atgaagatat aaacattcat tttattgaaa atatattatg cgtcgaacgt
    27541 ttatcaaaaa agaaggcgtc gtcatcacga cgctggcccg ttatttgttg ggtgaaaaat
    27601 gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca tcggttggcc
    27661 tgacgcaggc cgcgttgaaa acgttggaat caagtggagc gatacggatt gaacgccgtg
    27721 ggcgcaatgg cagttatctg gtcgaaatgg ataacaaagc gctgctgagt catgtggata
    27781 tcaataacgt tgtgtgtgcc atgcctttgc cctatacccg cttgtacgaa ggtttagcaa
    27841 gcgggctgaa agcccagttt gatggcattc ctttttatta tgcgcatatg cgtggcgcgg
    27901 atattcgtgt ggagtgtttg cttaacggtg tctatgacat ggcagtagtt tcccgcctgg
    27961 cggcggaaag ttatctcagc caaaacaatt tgtgcatcgc cctcgagctt ggcccgcata
    28021 cgtatgttgg tgaacatcaa ctgatatgcc gcaaaggtga gtctggaaat gttaagcgag
    28081 taggtctcga tagtcgttcg gcggatcaga aaatcatgac cgatgtcttt tttggcgata
    28141 gtgatgtgga acgtgtcgat ctctcttatc acgagagttt acaacgcatt gttaaaggcg
    28201 atgtcgatgc ggttatctgg aacgtggtgg cggaaaacga actgaccatg ctgggattag
    28261 aggcgacgcc gctcacagac gatccgcgat ttttacaggc caccgaagcc gtggtcctga
    28321 cgcgagtcga tgattaccca atgcaacaac tgctgcgtgc cgttgtagat aaacacgccc
    28381 tgctcgccca tcaacaacgg gtagtgagtg gggaacagga accgagttat taaacgaaag
    28441 gcatctgata tggaaaccag actcaacctg ctttgcgagg caggcgttat tgataaggac
    28501 atctgcaaag gcatgatgca ggtcgtcaac gtactggaaa cagagtgcca tctgccggtg
    28561 cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat gcgcagtcgc
    28621 cgtggtgaag aaatagagcc gctggataac gaattgctgg cagagctggc gcaatccagc
    28681 cactggcaag ccgttgtgca attgcatcag gtgttgttga aggaattcgc actggaagtt
    28741 aatccgtgtg aagaaggcta tttgctggcc aacctttatg gattatggat ggctgctaac
    28801 gaagaggttt gaagctcccg acaaatccac gtcgcacaac ctaaatattc aaaaaaatga
    28861 atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca ttgaaacgcc
    28921 agaacccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaag atcgccccgg
    28981 acagctgggt gatcgacgtg gatcaggtac tggaaaacgg taagcgactg attgagacgg
    29041 cgcggcttta cggcattgaa ctgtatctga tgaccaagca gtttggccgt aacccatggc
    29101 tggcggaaaa attgctggca ttaggttaca gcggcattgt ggcggtggat tacaaagagg
    29161 cgcgagtcat gcgccgcgct ggtttgtcgg tggcgcatca ggggcatctg gtacaaatcc
    29221 cttgtcatca ggttgctgac gccgttgaac agggcaccga cgtcatcacc gtgtttactc
    29281 tcgacaaagc gcgggaagtt tctgcggcag cggtgaaggc cggacgtgtg cagtttgtgc
    29341 tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc ggttttgtac
    29401 agcattcgct gcacgaggtg gttgctgaaa ttaagaaact gccgggtctg catttagccg
    29461 gacttaccca tttcccttgc ctgctttggg atgaggctgc cggaaaagtt ttgccgacac
    29521 cgaatcttca cacgctgata caggcacggg atcaactggc gaaatctggt attgcacttg
    29581 agcaactgaa cgcgccttca gcgaccagct gcacttcgct gccattactg gcgcaatacg
    29641 gtgtgactca tgccgaaccc ggtcatgcgc tgacgggcac tattccggca aaccagcagg
    29701 gcgatcagtc tgaacgtatc gcgatgctct ggttgagtga aatctcccat catttccgtg
    29761 gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcatgcgcaa catgcgttgg
    29821 tgtttacgcc agaaaatcaa aagattactg aaaccaatct caaaactgtg gatgacagca
    29881 gtatcgacta caccctgtcg ctggcaggcg agtttccggt aagcagtgca gtggtgctct
    29941 gtttccgcac gcagattttt gtcacccgta gcgatgtggt gctggtgtcc ggtattcacc
    30001 gtggcgaacc gaaaatcgtt ggtcgttatg acagtcttgg aaactctctg ggggcgtaat
    30061 ggcgcgattt gtggtgttag tgattgatag ctttggcgta ggggcaatga aagatgtcac
    30121 gctggtgcgt ccgcaagatg cgggagcgaa tacatgtggt cacatcctga gccagttgcc
    30181 gcatttgcag ctaccaacgc tggagaagct ggggctaatc aacgcattgg gttatgcgcc
    30241 aggcgatatg cagccgtcag attccgcaac ctggggcgtg gcagagctgc aacatgaagg
    30301 tggcgatacc tttatggggc atcaggaaat tttaggcacg cgcccgttac cgccgctgcg
    30361 gatgcctttt tgcgatgtga ttgaccgtgt tgagcaggca ttagtatccg ccggttggca
    30421 ggtggagcgc cgtggcgatg aactgcaatt tctgtgggtc aatcaggcgg ttgcgattgg
    30481 cgataatctc gaggcggatt taggccaagt ctataacatt accgccaatc tctctgtgat
    30541 ctcttttgac gacgcaatca aaattggtcg tatcgtgcgt gagcaggtac aggtcggtcg
    30601 ggtcattaca tttggtggcc tgttaaccga cagtcaacgc attctcgatg ccgcagaaag
    30661 caaagaaggg cgctttattg gtatcaatgc gccgcgttct ggcgcttatg acaacggctt
    30721 ccaggtcgtg catatgggct acggtgtcga taaaaaagtg caggtgccgc aaaaactgta
    30781 tgaggcgggc gtgccaaccg tgctggtggg taaggtggca gatatcgtca gcaatcctta
    30841 tggcgtgagc tggcaaaacc tggtggatag ccagcggatt atggatatca ccctcgacga
    30901 atttaacacc catccgacgg cgtttatttg caccaatatc caggaaaccg acctcgctgg
    30961 tcatgcagaa gacgtcgcac gttatgccga acgtttgcag gtcgttgacc gtaaccttgc
    31021 ccggcttgtt gatgcgatgc agccagatga ttgcctggtc gtgatggcgg atcacggcaa
    31081 cgatccgacc attggtcaca gccaccatac ccgcgaagtg gtgccgctgc tggtttatca
    31141 gcaaggcctg gctgctacgc agctcggtgt gcgcaccacg ctttctgatg tgggagctac
    31201 cgtgtgtgaa tttttccgtg cgccaccgcc acaaaatggt cgctcttttc tttcctccct
    31261 ccggtttgca ggagacaccc tatgagtttt gatccgacgg gttacaccct ggcccatgag
    31321 catctgcata ttgatctctc cggctttaaa aacaacgtgg actgccgcct tgatcagtat
    31381 gcgttcattt gccaggagat gaacgacctg atgacccggg gtgtacgtaa tgtgattgag
    31441 atgaccaacc gttacatggg gcgcaatgcg caatttatgc tcggtgtaat gcgcgaaacc
    31501 gggatcaacg tggtggcctg tactggttat taccaggatg cgtttttccc ggaacatgtg
    31561 gtgacccgca gcgtgcagga actggcgcag gagatggtcg atgaaattga acagggtatc
    31621 gatggcacgg agctgaaagc cgggctcatc gcggagatcg gctccagcga aggaaagatt
    31681 acgccgctgg aagagaaggt atttattgcc gctgcgctgg cgcataacca gaccggacgc
    31741 ccgatctcca cgcatacgtc gttcagcacc atggggctgg aacaactggc tctgttgcaa
    31801 gcccacggag ttgatctttc gcgcgtcacc gttggtcact gcgatctgaa agacaacctc
    31861 gacaacattt tgaagatgat cgatctcggc gcgtacgtgc agttcgacac cattggtaag
    31921 aacagttact acccggacga aaagcgcatt gcgatgctcc acgcgctacg tgaccgtggg
    31981 ttgctgaacc gcgtcatgct gtcgatggat attacgcgcc gctcccattt aaaagccaat
    32041 ggtggttatg gctatgacta tttattgacc acctttattc cgcaattgcg ccagtcagga
    32101 ttcagtcagg ccgatgtgga tgtgatgtta cgtgaaactc cctctcaatt tttccaataa
    32161 ggacagactc atgaaaaaga ttggcgttgc aggcttacag cgtgagcaga ttaaaaaaac
    32221 cattgaagcg acggctcctg gcagttttga agttttcatt cacaacgaca tggaagcggc
    32281 aatgaaagtg aaatccgggc aactggatta ctacatcggt gcgtgtaata ccggtgcggg
    32341 cgcggcattg tcgattgcca tcgcggtgat tggctataac aaaagttgca ccattgccaa
    32401 accaggcatt aaagcgaaag acgagcatat cgccaaaatg atcgctgaag gaaaagtggc
    32461 gtttggcctt tccgttgagc acgtcgaaca cgcgattccg atgctgatta accatctgaa
    32521 ataaaaggca cgactatgga tctgtatatt cagattatcg tggtggcgtg cctgacgggt
    32581 atgacatcgc ttctggcgca tcgctcggcg gctgtttttc atgacggcat ccgcccgatc
    32641 ctgccgcaac tgattgaagg ctatatgaac cgtcgcgagg cggggagtat cgcttttggt
    32701 ctgagcattg gttttgtggc ctcggtgggg atctctttta ccctgaaaac cgggctgctc
    32761 aacgcatggt tactctttct tcctaccgat atcctcggcg tactggcgat aaacagcctg
    32821 atggcgtttg gtcttggcgc tatctggggc gtgttgatcc ttacttgcct gttgccagta
    32881 aaccagctgc tgaccgcgct gccggtggat gtattaggta gcctcgggga attaagctcg
    32941 ccggtagttt ctgcttttgc actgttcccg ctggtggcga ttttctacca gtttggctgg
    33001 aagcaaagtc tggtcgccgc cgtggttgtt ctgatgaccc gtgtggtagt cgtgcgctat
    33061 ttcccacatc ttaaccctga atccatcgaa atctttattg gcatggtgat gctgctgggg
    33121 atcgcgataa ctcacgacct gcgtcatcgt gatgaaaatg atattgatgc cagcgggctt
    33181 tcggtgtttg aagaacgcac gtcgcggatt atcaaaaact taccctatat cgccatcgtg
    33241 ggagcattga ttgccgccgt tgccagcatg aagattttcg ccggcagtga agtgtcgatc
    33301 ttcactctgg agaaagcgta ctccgcaggc gtaacgccgg aacaatcgca aacgctgatc
    33361 aaccaggcgg cactggcaga atttatgcgc gggctgggtt ttgtaccgtt gattgccacc
    33421 accgccttag caacgggtgt gtatgcagtt gcgggcttta cctttgttta tgcggtgggc
    33481 tatctctcgc cgaatccgat ggttgcagcg gtattaggcg cagtggttat ttcggcggaa
    33541 gtcttgctgc ttcgttctat cggcaaatgg ctgggacgct acccgtcggt gcgtaatgcg
    33601 tcggataaca tccgtaacgc catgaatatg ctgatggaag tggcgctgct ggtcggttcg
    33661 attttcgcag caattaagat ggcgggttat accggattct ctatcgcggt tgccatttac
    33721 ttcctcaacg aatccctggg ccgtccggta cagaaaatgg cggcaccggt cgtggcagta
    33781 atgatcaccg gtattctgct gaatgttctt tactggcttg gcctgttcgt tccggcttaa
    33841 ggaaggcacc tatgaagacg tttcctctgc aaagcctgac gcttattgag gcgcagcaaa
    33901 agcagtttgc gctggtggac acgatttgcc gtcactttcc cggcgcggag tttctaacca
    33961 gcggtgattt gggcttaacg ccggggctga atcaaccgcg tattacccaa cgggtggagc
    34021 aggtgctggc tgatgcattt cacgcacagg ctgcggcgct ggtgcagggc gcggggactg
    34081 gcgcgattcg cgccgggctg gcagctttgc tcaaaccggg gcagcgtctt ctggtgcatg
    34141 acgcgcctgt ttacccgacg acacgggtta ttattgagca gatggggctg acgcttatta
    34201 ctgttgattt caatgacctg tcggcactga agcaggtcgt cgacgagcaa caaccggatg
    34261 cggcgctggt gcagcatacg cgccagcagc cgcaggacag ctacgtgctg gcagatgtgc
    34321 tggcaacgtt gcgcgcggca ggtgttccag cgttaaccga tgacaactat gcggtgatga
    34381 aggtggcgcg aatcggctgt gaatgcggcg cgaatgtctc gacattttcc tgcttcaagc
    34441 tatttgggcc agagggtgtt ggtgcagtgg tcggcgatgc tgatgttatc aaccgtattc
    34501 gcgccacgct ttactccggc ggtagccaga tccagggcgc acaggcgctg gaagtattgc
    34561 gttgtctggt gtttgcgcca gtgatgcacg cggtgcaggc aggggtatct gaacggttgc
    34621 tggctttgct taacggtggt gaggtgccgg aagtgaaaag cgcggtgatt gctaatgcgc
    34681 agtcgaaggt gttgattgtc gagtttcatc agccgattgc cgccagagtg ctggaagagg
    34741 cgcaaaagcg cggtgccttg ccttacccgg tgggtgcaga gtcgaaatat gaaatcccgc
    34801 cgctctttta tcgcctttcc ggaacgtttc gccaggcgaa tccacaatca gaacattgtg
    34861 cgattcgcat taacccgaat cgcagcggtg aagagacggt gctgcggatt ttgcgtgaga
    34921 gtattgccag tatttaatgc tggatagcac cgtcaagcta aattccgtac tgaacggtcc
    34981 cctcgcccct ttggggagag ggttagggtg aggggaacgt acgggatgta aaattcaacg
    35041 cgccagcatc gccggatgcg atgctggcgc atcttatccg gcctacggtt aatgtctttt
    35101 attatcaata gttaaggtga tgcgattggc gcggcagaag agttcggaga cgtgcaccgg
    35161 tttgtcattc tgatcaaagg cgattttgct gatgcgaaac aacggttcgc ccagttcgca
    35221 tttcagccat tttgcctgcg ggcgggtggc ggcaaagata tcgatggtct ttttgtcgct
    35281 gaccactcgc gtgtcaaaac gctcctgaaa taactgatag gtggagcttc cttcgacgta
    35341 aatctcatca aagtcaggat aacgcgacag cgggatccat gaactatcaa taaacagcgg
    35401 ctctttatcg agatacatca cccggcagag atggaacact tcgctgtttc cggggatgtt
    35461 cagctttttg caaaacggcg cggcgctgac ccgttcctgt tcgatcactt tctctttcgt
    35521 cgccttgcct tgtgagacgc caaaatcggt aaaaccactg acagtaagca gggcgttttc
    35581 aactttctgg ctttgtacaa aggtgccttt tccctgccag cggatcagta cgccgtccgc
    35641 cactaagtcg ctgatggctt tgcgaatggt aatgcggctg acgttatatt gtgtacaaag
    35701 ctcgttttcg gtagggatct gttgcccggc ctggtaaacc ccctgcgcga tatcatccag
    35761 cagtcgctgg cggacggtag cgtagaggag ttgatgagag tagcggtccg tagctgacat
    35821 aatcgaccct gaaataaaga atgaattact cggattatag ggtcgttttc cggcaagtaa
    35881 cagcacgttg gcgtgaagat gctcacgcca acgttatacc taccaggcac cgtggtactg
    35941 aatggttttc gccgcgcacg ccgttccctg cgccatcgcc tgcggtaatg tcatccccgc
    36001 agaccagccg caaaggaatc cggcaatgaa cgaatctccg gcacccatgg tgtcgataac
    36061 cgtcaccggt tcaggagcct gacgccagaa ctgcgcgcca tcccaggcaa tgctgccgtt
    36121 ttcacccagc gtgacaatca ctgttcctgc gccacgggca acaatcgctt tcatcttcag
    36181 acgcagcgtt tcgtcttctt gcggtgcgga ggcaaaggca aaatcgagat gcggcaccag
    36241 tgtctgccag agcgggctgt cccacttgtc ggagaagtcg aaagcggtaa gtttgcccgc
    36301 agcgtgcagc tgtgggaatg cgtcttccgc atgtccccag attgccgcgt gcacaatgtc
    36361 atactgcgcc agccaggcgt aatcctcttc actcagggca aagtcggcca tcacgccttc
    36421 ggtgtagtcg ccaaaaacgc gatcattgtc gtgcagttcc acctgagttt gtgcggtaac
    36481 gccgtgtttc gtatggacat ggctgatatc gacgcccatg cgggcgagat cctgcttcag
    36541 ctttgtgccg tagtcatcgt cacccaccca ggtaatgcat cccggctgta tgccgtagcg
    36601 agtgcagtac accgccacat tgaccgcatt accgccagaa aacgctttat tcagttgcgg
    36661 gtagatatcg acgcagttat cgccgattgt cgccagggtt ttcatctctc atcctccggc
    36721 agcagcgcgc gaaagcgttc cagcgcctgg cgggcataga gtctgggctc gttcatatac
    36781 atcgtcacca gctccaccgt acagtaaccc tcatagcccc gctcaataat atcgcgcatc
    36841 agttcccgca gcggcatttt gccttctcca ggaatgtaat gcgtgtcgct ggccccgtcg
    36901 ctgtcgacaa tatgcagatg acgtaattta tcgcccagtt tgtcgaaata actcatcacc
    36961 ggttccgcct ggacatacgg cgcgcaaatg tcgaccatgc tgaacaggcg cggcgaaggc
    37021 accagcgcca gcgcatgaag cacatcatta gcattacaca cgatgttcga ttcatacggc
    37081 gttaacggtt cgaggattag atccatgccg atgttttccg cgtactcata cagttcgctt
    37141 aggttctctg ccagccgtcc ccagataaca ttaggtggcg tgagatagcc cgcgtgggcc
    37201 gcggaaatca gcgtataacc cgcgttcatc tcttttgcca tatccatcgc cagcttgatc
    37261 atgtcgaggc tttcgcgacg catatgttca tcgcccagca tcatgttata cggatagcca
    37321 ttggtttctg gcgtatagcc gataatcggc atctgatacg tctgcgccag cgccttgatt
    37381 tgtttgatgc cgcccgcttt taagtccggc gcgaacgcgt gcgggcgacc gccccaaatt
    37441 tcgatgccgt cgtaacccag ctcgcttgca tcacgaaatg cgtgttcaat aggcagccgc
    37501 tggtggccgc aggtaaacat acctgttttt atcgggtaac tccttgcgta aatccggccc
    37561 gttgccgggc cgggcgaggg attaatattc caccagacca ccgtaatagc ggcgttcatc
    37621 cgggttgtga tctttgtaaa tagacaggta gtagcagagc cactccattg gcacgaacat
    37681 caggaacggt gccagccacg ggtgcagccc ttgcgaaatt tcggcgtaat cgatgacgat
    37741 cacgttgtca gtacgctgtt taacaaagtt aatggcgcgt tcggtggtgt ggcgactttc
    37801 atcattgccg agcaggaaca ggaacggaac gcccggttcg acaatctcca gcgggccatg
    37861 gcggaactct ccgctctcaa tcacgcagcc gtgcgtccag gtaaattcca tcagcgttac
    37921 aatgccttct ttgtaaccca gcggacgcag cggacccgca gcaacggtat aaatcatcgg
    37981 ccactggctg gccagttcac caagctggcg gcctttttct tcccaggtgc gtaccagatg
    38041 accgagcgca ttcggcaact gcttgagatc gtttttgatc ttgccgattt ccgcgttcgg
    38101 cgcgaggcgg gtgatcatct ccagcaccac gctgtagcag agcagcaggt gaatttccca
    38161 gatacagtcg gcctgataat caatgctaaa ttccgccgcc gaggtaatcg ggctgtccgc
    38221 gcgtttggtg aacgccgcag tgagtgcgcc gcaggcccgg cccagctcca gcgctttgat
    38281 tacctcttcc gttttaccgt agtcagaaac gccaatcacc gcgcaacgat cgtcgaggcg
    38341 atacggggtg ttatcgcaga actcccagcc ggaaatggcg tagacctgaa gatcggaaaa
    38401 gcgatccgcc agatgtttcg ccgcttgcgc cgcgttgagt ggcgagccgc aggcaacgaa
    38461 ataaatacga tcaatgtcgc gcttcaccat ctcttccacg atggcgtgca ccagcggaac
    38521 gtcatggctg agaacttttt ccacttcctg aaccatattt tcggtgacca gaaagtccac
    38581 ggtgctttta tcaatatcca acattttatt ttctccagat aacattacga caaagcgttg
    38641 agctggcggc tacgcttcgc ccagaaggcg taagcaggca gaccagtagc aataacgatg
    38701 acggcgcaga taaggccggg aatcggtgcc cagacaaagg ttgaggcgac cagaatgagg
    38761 cttgacgcaa tggcgagggt ggtcatcagc ccgaaagcag gagtacgcca cagcggtttg
    38821 taatcgtcgc gtttacgaca ccagatgatg gagccgaagg tgagggtatt tttgaaacac
    38881 atcaccaggg tgaaataacc cagcaggctg gtgagatcgg aaacgaagat gaagaagagc
    38941 cccagcgccc cttgcaggat gatggagaca tccggcgtgt tgtatttcgg atgcacatgg
    39001 ccgaagcatt taaagaacag gttgtctttc gccatcgcgt attccagccg cggctggtac
    39061 atcacgcagc tggaaagcga accaagaatg acgatcatcg ccgtgatggc aacaaagatc
    39121 ccagcggtgc tgccgagtgc ggggatccag gtcagggcgt cggaaatcgg cgtttcagaa
    39181 ttggcgagtt tgtcgaaggg catcaggtcg gaaatcacca gcgccagcag ggtgtagagc
    39241 accagaacca gcagacagga accaatcagc gctcgtggca tggtttttcc ggggttttta
    39301 atttcgccgg tcatataaca gatagaggcc atgccggtat acgaccaact ggtggcagag
    39361 atccccgcca gcagcgccat aaagctgccc gttgcgccaa tcgcagtggt ggtaggggcg
    39421 gcaaaattct ccgctttaaa ccagaagatc ccaaggccaa tgacgatagt gaacgggata
    39481 attttggcga tggtaattag cgtctgaaac gctgcgccgc cttcaacgga gcgcaggtgt
    39541 agcaacataa aggcgataat taatccggcg gcgataaatt taccgggcaa cggatcgata
    39601 ggcgttaaaa agccaagatt gctgacaatc gccagcgcca taatcgacaa tgacggcgca
    39661 tcgttggccc agaagctggc ccagccggag aggaaagcca gcggtcggct tccggcattt
    39721 ttcagataaa cataatctgc gccattttcc ggataagcgg tggatagttc cgcatagacg
    39781 cacatttgcg ggatcacaat taacccgcca atgacaaacg tgagcaccgt aagccacggc
    39841 gtgcccgctg cttttgccac ttcacccaca gatacaaaaa taccggagcc gacggttgtc
    39901 ccgacggcga ttgcaagaac ggcccaaaat ccgagcttgc gttggagttc ctggcttccc
    39961 atagcgatac ctttttcttg tccaacacgt tgtaggggag agtttgcagt catttaaaag
    40021 gtaacatgac aatgcatgat gaatataata acatatgatg ttatgtaatg cggaggaaga
    40081 tcacaaaatt tcgcacagga tcgcgctgtg gctaatgaat gtagttatcc a
//
LOCUS       DAJTYD010000015        39846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTYD010000015 DAJTYD010000000
VERSION     DAJTYD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.015"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(347..2038)
                     /gene="fruA"
                     /locus_tag="OO585_000719"
     CDS             complement(347..2038)
                     /gene="fruA"
                     /locus_tag="OO585_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6628402.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2055..2993)
                     /gene="fruK"
                     /locus_tag="OO585_000720"
     CDS             complement(2055..2993)
                     /gene="fruK"
                     /locus_tag="OO585_000720"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6628403.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2993..4123)
                     /gene="fruB"
                     /locus_tag="OO585_000721"
     CDS             complement(2993..4123)
                     /gene="fruB"
                     /locus_tag="OO585_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6628404.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4491..5672
                     /gene="setB"
                     /locus_tag="OO585_000722"
     CDS             4491..5672
                     /gene="setB"
                     /locus_tag="OO585_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6628405.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5669..5923)
                     /locus_tag="OO585_000723"
     CDS             complement(5669..5923)
                     /locus_tag="OO585_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6628406.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6078..6650
                     /gene="yeiP"
                     /locus_tag="OO585_000724"
     CDS             6078..6650
                     /gene="yeiP"
                     /locus_tag="OO585_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6628407.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6873..8339
                     /locus_tag="OO585_000725"
     CDS             6873..8339
                     /locus_tag="OO585_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6628408.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8457..9443
                     /gene="yeiR"
                     /locus_tag="OO585_000726"
     CDS             8457..9443
                     /gene="yeiR"
                     /locus_tag="OO585_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6628409.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9482..10195
                     /gene="lpxT"
                     /locus_tag="OO585_000727"
     CDS             9482..10195
                     /gene="lpxT"
                     /locus_tag="OO585_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6628410.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10607..11173
                     /gene="mepS"
                     /locus_tag="OO585_000728"
     CDS             10607..11173
                     /gene="mepS"
                     /locus_tag="OO585_000728"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6628411.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11354..12910
                     /locus_tag="OO585_000729"
     CDS             11354..12910
                     /locus_tag="OO585_000729"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6628412.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            12992..14806
                     /locus_tag="OO585_000730"
     CDS             12992..14806
                     /locus_tag="OO585_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6628413.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14807..15901
                     /locus_tag="OO585_000731"
     CDS             14807..15901
                     /locus_tag="OO585_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6628414.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            15901..16926
                     /locus_tag="OO585_000732"
     CDS             15901..16926
                     /locus_tag="OO585_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6628415.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            16928..18517
                     /gene="yejF"
                     /locus_tag="OO585_000733"
     CDS             16928..18517
                     /gene="yejF"
                     /locus_tag="OO585_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6628416.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18521..18865)
                     /locus_tag="OO585_000734"
     CDS             complement(18521..18865)
                     /locus_tag="OO585_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628417.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19198..20381)
                     /gene="bcr"
                     /locus_tag="OO585_000735"
                     /pseudo
     CDS             complement(19198..20381)
                     /gene="bcr"
                     /locus_tag="OO585_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20409..21104)
                     /gene="rsuA"
                     /locus_tag="OO585_000736"
     CDS             complement(20409..21104)
                     /gene="rsuA"
                     /locus_tag="OO585_000736"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6628418.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21253..23013
                     /locus_tag="OO585_000737"
     CDS             21253..23013
                     /locus_tag="OO585_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6628419.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23138..23422
                     /gene="rplY"
                     /locus_tag="OO585_000738"
     CDS             23138..23422
                     /gene="rplY"
                     /locus_tag="OO585_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6628420.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23561..24568)
                     /gene="yejK"
                     /locus_tag="OO585_000739"
     CDS             complement(23561..24568)
                     /gene="yejK"
                     /locus_tag="OO585_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6628421.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24750..24977
                     /locus_tag="OO585_000740"
     CDS             24750..24977
                     /locus_tag="OO585_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6628422.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            24997..26757
                     /gene="pbgA"
                     /locus_tag="OO585_000741"
     CDS             24997..26757
                     /gene="pbgA"
                     /locus_tag="OO585_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6628423.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26832..26908
                     /locus_tag="OO585_000742"
     tRNA            26832..26908
                     /locus_tag="OO585_000742"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26866..26868,aa:Pro,seq:ggg)
     gene            27127..28368
                     /locus_tag="OO585_000743"
     CDS             27127..28368
                     /locus_tag="OO585_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6628424.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28864..29070
                     /locus_tag="OO585_000744"
     CDS             28864..29070
                     /locus_tag="OO585_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6628425.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29085..29750
                     /locus_tag="OO585_000745"
     CDS             29085..29750
                     /locus_tag="OO585_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628426.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29776..29976
                     /locus_tag="OO585_000746"
     CDS             29776..29976
                     /locus_tag="OO585_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6628427.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            29966..30187
                     /locus_tag="OO585_000747"
     CDS             29966..30187
                     /locus_tag="OO585_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628428.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30180..30413
                     /locus_tag="OO585_000748"
     CDS             30180..30413
                     /locus_tag="OO585_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628429.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30406..30639
                     /locus_tag="OO585_000749"
     CDS             30406..30639
                     /locus_tag="OO585_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628430.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30645..30944
                     /locus_tag="OO585_000750"
     CDS             30645..30944
                     /locus_tag="OO585_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6628431.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            30941..32341
                     /locus_tag="OO585_000751"
     CDS             30941..32341
                     /locus_tag="OO585_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6628432.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32541..32792
                     /locus_tag="OO585_000752"
     CDS             32541..32792
                     /locus_tag="OO585_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628433.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32789..33199
                     /locus_tag="OO585_000753"
     CDS             32789..33199
                     /locus_tag="OO585_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6628434.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33210..33482
                     /locus_tag="OO585_000754"
     CDS             33210..33482
                     /locus_tag="OO585_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6628435.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33721..33927
                     /locus_tag="OO585_000755"
     CDS             33721..33927
                     /locus_tag="OO585_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628436.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            33927..34982
                     /locus_tag="OO585_000756"
     CDS             33927..34982
                     /locus_tag="OO585_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6628437.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            34994..35329
                     /locus_tag="OO585_000757"
     CDS             34994..35329
                     /locus_tag="OO585_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6628438.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35342..35755
                     /locus_tag="OO585_000758"
     CDS             35342..35755
                     /locus_tag="OO585_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6628439.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            35917..36351
                     /locus_tag="OO585_000759"
     CDS             35917..36351
                     /locus_tag="OO585_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628440.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            36632..36913
                     /locus_tag="OO585_000760"
     CDS             36632..36913
                     /locus_tag="OO585_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628441.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(37592..>39846)
                     /gene="yejO"
                     /locus_tag="OO585_000761"
     CDS             complement(37592..>39846)
                     /gene="yejO"
                     /locus_tag="OO585_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6628442.1"
                     /translation="LNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVK
                     EGGLAIAVDQKAGGAIKASTRVMEAFGTNRLGQFEIKNGIANNMLLENGGSLRVEEND
                     FAYNTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSK
                     NYELDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSIT
                     YSSKAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSE
                     GASFRTHGAVDTSKADVSLENSVWAIIADITTTNQNTLLNLANLAMSDANVIMMDEPV
                     TRSSVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTG
                     ASPAAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITP
                     STTDVLNMAAAQPLVFDAELDTVRERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGF
                     EQTLTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEH
                     QNGAYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPY
                     LAFTGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVR
                     QEYADSNHVKVNDDGKFNNDVAGTPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPW
                     NTQAGVVWTF"
BASE COUNT        10014 a        10135 c         9989 g         9708 t
ORIGIN      
        1 gtgttcatat ttaaacatgt gctcatagca tgcatgtttt acgtaaagta aattaccgcc
       61 agagaggcaa aatgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta
      121 caaaagcgag gaatttgtga cgggatgcac agaacgggaa caataaaaaa ggcagaaaat
      181 aatctgccct tgaggaaata accttagtgt ttgatgaacg tttcaagttt ctactgaaag
      241 ataaaaccat ttaaattcat aaaattacat tcacaaaccg gtcattctct ttgcgctcat
      301 caaatgttac agggcaggaa atttctgccc tgtaacacac cttttattac gctgctttcg
      361 ctactgcgtc cacttccgga cgtttcagga aggcataggc caaacccgcc accagcgtac
      421 cggcaataat tgctaccagg taacccagta ccggcgtaat agcgccaggg atcagcagaa
      481 caaacagacc accgtgcggt gccatcagtt tcgcaccaat cgccattgag attgcgccag
      541 tcagcgcccc acccacgata cagcacggca gcacacgcat cggatcacga gcagcaaacg
      601 gaattgcacc ttccgaaatg aagcacagtc ccaataccag agcggctttg ccaccttcct
      661 gctgcgcttt gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg
      721 gcaccatacc tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac
      781 ccacaccgaa tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc
      841 caccgaggat cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag
      901 tcagcccttc gagaatgcca gcaactggtt taccgatcag gtagatcatc gccagaccga
      961 ccaccagact ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg
     1021 gcagtttcag ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta
     1081 taccgccaat aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggcg
     1141 tgaggcccgg acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca
     1201 gcgcaaaggc tgaaccaccg ccaatctgca tcagcgccgc tgccaacgta cccggctctt
     1261 taaacgcttc gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcgacgacca
     1321 tcggcagcat ataagagacg cctgtcagca agtgacggta agcgcctgca ctctctttct
     1381 taccttcagt ggtcgccgtt tgagctttgc ccgccggttc atacggcgtt gcttcagcaa
     1441 ccgctttatc cagttcctgc gcggttttct tcagcgccag accggtagag gtacgataca
     1501 tcggtttacc agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat
     1561 ccgctgctgc gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt
     1621 caactttcac ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa
     1681 aggtgtgtgc tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg
     1741 cagcaaccgg tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt
     1801 cactcaggaa cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt
     1861 taccgttcag cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt
     1921 cgttcggatt gtcgatgatt tccagttttg cttttcgcgc caccgcgccc agcagggtct
     1981 tcgccatata ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta
     2041 tgcctctcct gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac
     2101 tgcggacgat cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc
     2161 gccagacgca gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca
     2221 accatagaat cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc
     2281 cattcgccgg aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc
     2341 gcaatacctt gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt
     2401 ttacggcctg cccaaatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct
     2461 ttcaaacctg ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta
     2521 cgcaggcgag tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg
     2581 ctgacacaga ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc
     2641 cagtcggcgg gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt
     2701 tccgtcagct taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg
     2761 cccagctcgc tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca
     2821 acggtgacat caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc
     2881 gcatgcagac cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa
     2941 ccaacaaggt cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc
     3001 cctccccaag accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg
     3061 caccctgggc ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca
     3121 tcagactacg tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac
     3181 tgttaaattg tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt
     3241 cattgcgcac cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca
     3301 gcagcgccag caacgttgcc gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca
     3361 ataaatcagc gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca
     3421 ccagcatggc tgccgtttcg ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa
     3481 tcgcgctacg cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca
     3541 gcggttgttc attgatggct ttggtgacga aagtggcgtc aactgccccc gcctctttca
     3601 gacgcgcagc gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca
     3661 gcatttcgtt gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa
     3721 gttcttctgc tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt
     3781 gggtcagctg gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca
     3841 cgtacgctac ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc
     3901 cggttttcag cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc
     3961 cgaggaacgt tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta
     4021 cattaccggc ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg
     4081 ccttttcgcc cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct
     4141 gaattgaaac gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga
     4201 caagatcgcg ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa
     4261 gccgtaaagt tatgagcaaa agtttgatgt gctgcacatt tttttgtcat ttttcctaat
     4321 tgctgatggg aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc
     4381 aacagtgaaa tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt
     4441 ctgacaagca gcgtaaactc cgcgtcttcc tcttccagtg atagacctgc atgcataact
     4501 cccccgcagt ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg
     4561 cctttctcac cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg
     4621 atgaagtaca tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg
     4681 ggattctggt aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc
     4741 tgattgtctt ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca
     4801 actactttgt tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc
     4861 cgcaaatgtt tgcccttgcc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt
     4921 tcagctcttt tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt
     4981 atgcgttagc gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta
     5041 ttgtttgcgg cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg
     5101 cgaccggcac ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca
     5161 tttgtacatt gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta
     5221 tcaacgaact gcatcttccc gagaaactgg ccggtgtgat gatggggacc gccgccgggc
     5281 tggaaatccc gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct
     5341 taatgcgcgt tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt
     5401 cacctgccat tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg
     5461 gcatcgggat gctctatttt caggatttga tgcccggtca ggcaggttca gccaccacgc
     5521 tctataccaa cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg
     5581 ccgagatctg gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc
     5641 tgttttgctt actgcggact aaagatgttt aagacgcggt cagcatttcc agatcgataa
     5701 gccaggtcat ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg
     5761 cacagacttt cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct
     5821 gaatgcaggg cgtattggcg ggcttgccat ctggcatacc gggaataggg ctgaaaattg
     5881 aaggggcagt gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg
     5941 ttaaccaccg gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga
     6001 ataccacttg cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg
     6061 actacaggaa tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt
     6121 acaacggcaa actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg
     6181 ccgctacgct gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc
     6241 gtttcaaagg tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct
     6301 cctatgtcga tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct
     6361 tcaccaaaga tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca
     6421 tgcaggtgct gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc
     6481 tggaaatcgt tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac
     6541 cggcgacatt gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa
     6601 aaattcgtat ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc
     6661 atgcagaaaa gggatagctc aggctgcccc ttttttaatt tattatacca atcttctatt
     6721 ttgcgcttcc tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac
     6781 ttcatcgcat taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat
     6841 tgttaattta tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc
     6901 cgcatcatgt acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt
     6961 ttggctttgg cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg
     7021 cccagggcgg cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga
     7081 gccagctccg cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc
     7141 aggtgataat tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg
     7201 caattattga gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa
     7261 aaggctattg tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc
     7321 acgatctaca aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga
     7381 aacgccgccg tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg
     7441 acaatggcca tgtggtgaaa aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac
     7501 tcgccgggtg gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc
     7561 cggctgcaac cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct
     7621 gcgcgattag ctgcgaaccg tttatccagt gggtggtgga agataacttc gtcgctgggc
     7681 gtcctgcctg ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga
     7741 tgaaactgcg tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctttcag
     7801 gattcgccca tatcagtgat tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat
     7861 taatgctgaa tgagcaagcg ccgacactac aaattaaaga tgtcgattta acacaatatg
     7921 cggacaagtt aattgctcgt tttgctaatc cggcgctgaa acataagacc tggcaaatcg
     7981 caatgaatgg tagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg
     8041 ggcgcgaaac ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca
     8101 gcggcgttga tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc
     8161 gcgaacttgt tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg
     8221 aagttttcgg tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct
     8281 ggcaacaaat cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac
     8341 gatttctgcg gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg
     8401 ccttttttag ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga
     8461 ccaggaccaa cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc
     8521 tgttagccca taaagatccc aacgaaaaat ggacggtact ggttaatgaa tttggggaag
     8581 tcggaattga tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg
     8641 gctgcatgtg ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc
     8701 agggaaaacc agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc
     8761 tcgatctgtt aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca
     8821 ttctcgatcc acgcttgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc
     8881 agctggctgc cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg
     8941 agcaagcgct acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg
     9001 agcatgggaa agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc
     9061 ccgccagcgc cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc
     9121 tgccagagca tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg
     9181 gctggatatt cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac
     9241 ttgcaccggt ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca
     9301 accgtcaggg cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta
     9361 ttgagctgat ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc
     9421 ttcgtttagc gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa
     9481 tatgattaaa aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct
     9541 ttcctggtat attcccgtta atcatggatt atggttgccg attgatgcgg atatttttta
     9601 tttctttaat cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgaccaa
     9661 caatcgcgct ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg
     9721 gctgaaagaa aacgccccag gcagacgacg tatcgtgatt attggtctgg taatgctatt
     9781 aactgcagtg gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc
     9841 aacattgacg tttactaata ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga
     9901 tgcctcacga gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt
     9961 catgtggcgt tatttcggta aagttgcagg tcttatcgcc cttattattt ttgtggtttt
    10021 tgcgtttccc agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat
    10081 gaccgtgata ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat
    10141 cacctttttt gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac
    10201 agtaattaac atcatcaggt ttatttttat agtgaggata atcctgatga tgcgcaccgt
    10261 gctttcatct atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt
    10321 cactcaatcg tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt
    10381 ataaaaaatc atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt
    10441 tttgcctcgg cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt
    10501 ggccttaagg aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt
    10561 ttataacgat atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca
    10621 accgattttg agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc
    10681 tgcatgtagt gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag
    10741 tgaaacatca tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga
    10801 cgtaaaatcg cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg
    10861 cggcagcact aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca
    10921 atttggctta gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc
    10981 ccgcagtaat ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca
    11041 tgtcggtatt tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat
    11101 tatttccagc atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgagttct
    11161 cagccgcagc taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga
    11221 aagcactgct taggtagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg
    11281 tcttgatcca ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga
    11341 aattaacgga atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg
    11401 cattgtagca ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg
    11461 gcataagcac gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac
    11521 ctattttgcc gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg
    11581 ccagcaggct aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt
    11641 tgtgctggtg aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc
    11701 actcaatgaa ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc
    11761 cggcacgccg atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct
    11821 gaaaaatagc ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag
    11881 ttcacggcag gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac
    11941 cttttcttca cgtttgatga acgttaatga attaaccgac atgcctgtcc gtgaaactaa
    12001 aattgcgggc attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt
    12061 gtggtacgca tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta
    12121 cctgatgagc gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca
    12181 attttacgtg gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga
    12241 agtactgcta cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa
    12301 ctttgccgaa tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc
    12361 ccgcgatgcc gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat
    12421 tgcgccggac catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc
    12481 cctgcccgca caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga
    12541 gcaagaagcc acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga
    12601 tgacttcggc accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct
    12661 gaaaattgac cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact
    12721 tgacgcggtg ctgacgctgg cgaaacgcct caatatgctg acggttgctg aagggggcga
    12781 aacgccggaa caggcgcgat ggctaagcga acccggcgtt aatttcatgc aaggctactg
    12841 gattagtcgc ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc
    12901 gcagtggtaa ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg
    12961 ctaatgatga gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat
    13021 cgccctgttc accttcggtg cgcaggcgca ggctatcaag gaaagctatg cctttgccgt
    13081 gctgggcgaa ccccggtacg catttaattt caaccatttt gattatgtga accccgccgc
    13141 gccaaaaggt gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta
    13201 tgcactgcgc ggcaacccgg gcgcacgcac cgagcagttg tacgacacgc tatttacgac
    13261 ttccgatgac gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga
    13321 cgattattcc tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc
    13381 cattactgcc cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca
    13441 atttcgtctg gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat
    13501 tgagttagct aaacccggca aagaagatat gctgagtctg ttttcgctgc cggtatttcc
    13561 agaaaagtac tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag
    13621 tggtccgtac cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa
    13681 agattactgg gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg
    13741 ctacgattat tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga
    13801 tttgcgtatg gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga
    13861 taaaaaatac atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct
    13921 ggcgtttaat atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct
    13981 cgcctttgac tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa
    14041 cagttatttt cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt
    14101 gcttctggca ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc
    14161 gccggtatct aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acacacttct
    14221 caacgaagcg ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca
    14281 actcagcttt gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca
    14341 gcacagcctg caacggctgg gtatcaacat ggacattcgc aaggtggata actcgcaaat
    14401 caccaaccgc atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc
    14461 gtggcccagt tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa
    14521 tgcccccggc gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca
    14581 gggaaataaa gaaaaattat tgccgttggg gcgagcactg gatcgcgtat taacgtggaa
    14641 ttattacatg ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt
    14701 ctcccacccc gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt
    14761 taataaagcg gccaaactgc cgtcagccag gcaacaggga gagtagatgg gcgcttacct
    14821 gattcgccgt ctgttgctgg tgatcccaac attatgggcg attatcacca tcaacttttt
    14881 catcgtgcaa attgcgcctg gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg
    14941 taatgccgga gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac
    15001 gggtgtcggc aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt
    15061 gatcgctgag atcactcatc gctacggttt tgataagccg atccacgaac gttacttcaa
    15121 aatgctctgg gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt
    15181 gctgacgctg attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct
    15241 gattatctat ctggtgtcga ttccgttagg cattcgcaaa gctgttcata atgggagccg
    15301 ctttgacgtc tggagtagcg catttatcat tatcggctac gccattccgg cctttttgtt
    15361 tgccatcctg ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg
    15421 cggcctggtt tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct
    15481 gtggcatatc acgctgccgg tgctggcgac ggtgattggt ggctttgcgg cgctgaccat
    15541 actgacaaaa aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc
    15601 gaaaggggta agtgaaaaaa atattctctg gaaacatgtg ttccgcaacg ccatgctgct
    15661 ggtgattgcc ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat
    15721 tgaggtgatg ttttcactca atggtctggg cttactgggc tacgaagcga ccgtctcgcg
    15781 cgattatcct gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa
    15841 tattgtcagt gatatcagct atacgctggt tgatccgcgt attgattttg agggacgtta
    15901 atgtcgcgac tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc
    15961 ggctactggt cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg ttctgagctt
    16021 atcgccaacg ataaaccatt gctggtacgt tatgacggca gttggtattt cccgttgttg
    16081 aaaaactaca gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac
    16141 ccgtggctga aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt
    16201 ggtgctacca gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa
    16261 aactggctgg gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg
    16321 cggatctcgg ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg
    16381 gcgggggcgc tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt
    16441 gaagtatggt cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag
    16501 cctaacttct ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc
    16561 gtggtgcggg cggagttttt acgtactcgt aatttcgact acattcgtgc ggcgcaggcg
    16621 cttggcgtca gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg
    16681 accctcacct ttttaccgtt tattttatgt agttcgatca ccaccctgac ctcactcgat
    16741 ttcctcggct tcggtctgcc gctcggttca ccgtcactcg gtgaactgct gttacaaggg
    16801 aaaaataacc ttcaggctcc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta
    16861 ttgtctttgc tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg
    16921 gtgtagcatg acgcaaactc tgttagcgat tgaagatttg tcggtgggtt ttcgccatca
    16981 gcaaaccgta cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct
    17041 ggcgctggtg ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct
    17101 gctcccttcc ccgccggttg aatatctctc cggcgatatt cgttttcgtg gcgaatcgct
    17161 gcttcatgcc agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt
    17221 tcaagagccg atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt
    17281 gctttcactc caccgcggga tgcgtcggga agcggctcga ggcgaaattc ttaactgcct
    17341 tgatcgcgtt ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc
    17401 cggcggcgaa cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt
    17461 aattgccgat gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct
    17521 gttgcgcgaa ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag
    17581 cattgtcaga aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca
    17641 aaattacgcc gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa
    17701 cagtgaaccg tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt tgctggatgt
    17761 tgaacagctt caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca
    17821 taatgtggtg gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac tgggtttagt
    17881 gggcgagtcc ggttccggga aaagtacgac gggactggcg ctgctgcgac tgattaattc
    17941 tcagggcagc atcatctttg acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt
    18001 acctattcgt catcgcattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg
    18061 actcaacgtt ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc
    18121 cgcacaacgc gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac
    18181 acgccaccgt tatccggcgg agttctctgg tggtcagcga caacgtattg caattgccag
    18241 ggcattaatt cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa
    18301 aacggtacag gcgcaaatat tgacgctatt gaaatcattg caacaaaagc tgcaactggc
    18361 ctatttgttt atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt
    18421 actgcgacaa ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca
    18481 gcaggagtat acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg
    18541 aaaagggttc ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt
    18601 cctggcgatt tacaaacagg cacggctcac cttcacactc cagcaccgaa cacggaactt
    18661 cgatgtcgct taacgcctgg cttagtttgt acatcaccga ccatgcatta tcaccatccg
    18721 ggctaagcaa tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg
    18781 gttcaacctt cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga
    18841 cgattgagag ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt
    18901 gtttacatca attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga
    18961 tgcctcgcct gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg
    19021 tttgcggggc tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat
    19081 gtgacttttt acataaattg attttacata aaataaacat atatcggggg aatgatagat
    19141 ttgtggttga catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca
    19201 ccgttttttc ggtcgactgg cgtacagaca gaagagaatg gagctgcttg cgcagaatgc
    19261 aattgaccaa atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac
    19321 aattgcccct atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg
    19381 gggaaactca tcaagaatga ccgccatcgc attggatgac accatcgaca cacagcccac
    19441 aaacgccgca acgccaacca ccagaatccc agccccagca gcgcactgat gaccatccac
    19501 gctgccataa taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg
    19561 cgaacgaagc ggctgttaaa gatggtcatc acgaacagga aaacaatgtt tagtgcaaag
    19621 taataaccaa agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg
    19681 ctcaggaacg agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca
    19741 cgcttatgac ggaacagcgc cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg
    19801 cgacgctccg gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc
    19861 gccgccacgg cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg
    19921 ccaactatcg gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc
    19981 cgcgagaact cttctttcgg gtagatatca cgcatcaaag cgttgataac catgctggcc
    20041 gcagccgcag ccagcccgtg gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc
    20101 aacgcacacg ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta
    20161 cgcccgaagc tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata
    20221 taagtactga gggtcatctg cgtactgccc gccggtacgc caaactgcgc tgaaattacc
    20281 ggtagcgcgg gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg
    20341 ataaaaacaa tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca
    20401 ttgaaaagtt agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg
    20461 gctaaatcag catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg
    20521 ttacccacgg cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga
    20581 cgaacctgcg ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta
    20641 tgcagctgca cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt
    20701 gtcaccagat aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga
    20761 ccatcatcag tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc
    20821 agtttccagg ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc
    20881 gtggagcaaa catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc
    20941 gccagcgggt tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta
    21001 cgaacaattt cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca
    21061 cggctaacgc cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct
    21121 gtaaaaacgg aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg
    21181 gtctaataac atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac
    21241 ctacgcgaga ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg
    21301 ctcaaccatt ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt
    21361 aaaagcctgg tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca
    21421 cacgtcaaag aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc
    21481 gatatttttg ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc
    21541 gtgcagtcgg tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc
    21601 gatgaatgcc accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac
    21661 ctgactaaag tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg
    21721 ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc
    21781 cttttccgtg actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg
    21841 acgccgccag aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca
    21901 cagagtaacg ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc
    21961 attacgccac atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg
    22021 attttcgccg ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat
    22081 gcagcactga ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt
    22141 aaagcccagc gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc
    22201 ccgcacgtcg atcttatcgc cattctgcgt cctaccgaat cagtgagtct ttaccaacaa
    22261 attgtcgggc gcggtctgcg tctcgctcct ggcaagactg attgcttaat tcttgattat
    22321 gcgggtaatc ctcacgacct ctacgcgccg gaagttggta caccgaaagg caaaagtgac
    22381 aacgtcccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa
    22441 acgaccgccg acgggacatt gattgaacac tttggtcgtc gctgtcaggg atggtttgaa
    22501 gatgacgacg gccatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa
    22561 tgtaacgcgg aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt
    22621 gatccggatg atatgttaaa agcggcgcta cgactgaaag acgcgctggt attacgctgt
    22681 agcggcatgt ctttacagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat
    22741 tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga
    22801 actgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc
    22861 tggatcaccg ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc
    22921 gtcgcccgca tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt
    22981 cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg
    23041 attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg
    23101 ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt
    23161 acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc
    23221 aatcatctac ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat
    23281 gaacatgcaa gctaaagctg aattctacag cgaagttctg accatcgttg ttgacggtaa
    23341 agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca
    23401 catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg
    23461 ttttttgtat ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa
    23521 ggcgttcacg ccgcatctgg cattcagaat acaaaaccga ttaattgccg ccagacgtcc
    23581 ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg
    23641 ctggatccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac
    23701 cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct
    23761 cttccagttc gtagcctttt tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg
    23821 ccagctcttt cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac
    23881 agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg
    23941 cagtgaaatc atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt
    24001 cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca
    24061 ggaaagtgag atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg
    24121 caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt
    24181 taacgcgcat actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat
    24241 aacggtagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt
    24301 cacgcaggcg cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct
    24361 gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt
    24421 tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca
    24481 gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca
    24541 gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc
    24601 aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta
    24661 acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg
    24721 ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac
    24781 aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg
    24841 acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc
    24901 cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg
    24961 aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac
    25021 cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg
    25081 ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct
    25141 ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc
    25201 tacttgctga tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt
    25261 ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt
    25321 actcgtttcc atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa
    25381 aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt
    25441 gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc
    25501 ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat
    25561 atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt
    25621 tcgtacccga tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag
    25681 tatcaacgcc gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta
    25741 agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat
    25801 ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa
    25861 aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc
    25921 ctgttctatg gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacccctgcg
    25981 gcgttaatta ctgcgcttaa tcagcaaggc tatcagctgg gattattctc gtcagatggc
    26041 tttaccagtc cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc
    26101 ccccaatccg acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa
    26161 gaagataacc gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat
    26221 cagcaggcat ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac
    26281 cgcgtgctca atgcactgct tgattctggc aaactggaca atacggttgt gattatcact
    26341 gccggtcggg gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat
    26401 ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg
    26461 actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct
    26521 gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtc
    26581 actgcagcgg ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac
    26641 aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca
    26701 cagttaagtt tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta
    26761 attataaatc agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag
    26821 cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc
    26881 ggaggttcaa atcctctcgt gccgaccaaa tcgcccaccg gataccaccg gaattaacag
    26941 tttttagaca cgaaaagccc gctaatcctt aattagcggg tttttttatg gtttttagtc
    27001 accggacatc accggattta taagctatca gccggatttt ttagttatac gcttagttat
    27061 acgggcttgc gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat
    27121 atacatatgg cgagaaaaac acccccatta accacggtgc agatcaaagc agccagacca
    27181 gcggaaaagg aatacaccct acaggacggc ggagggcttt ttctcctggt caaaccgtcc
    27241 ggatcaaaac tctggcgatt ttcctactac cggccatcgg acaaaaaaag aatattgctg
    27301 agttttggat cgcttgatga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac
    27361 agagcgttaa tcagtgccgg aactgacccg cagggccatg agaagaaaaa acgagaggca
    27421 gaggcccgaa gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa
    27481 atcagccaga atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat
    27541 gtattcccgt tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa
    27601 gtgcttacac ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc
    27661 gttaatgagg taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg
    27721 aatgtgcgta aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa
    27781 cagctgccgg agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta
    27841 ctgattgaat ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg
    27901 gatgaaatca acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc
    27961 agggatcacg ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca
    28021 atcagccacc accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac
    28081 agccagacag ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat
    28141 ggattacgcg ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata
    28201 gaggcggcac ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac
    28261 tacatagaaa aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg
    28321 acgggcgtaa ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa
    28381 ccagtaaaaa ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca
    28441 aggccgtgaa acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc
    28501 tgcaactggc cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa
    28561 cgacggcccc atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa
    28621 atctggcggt aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc
    28681 ccaaaaaatg gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca
    28741 tttcaaatca tcaatataaa aacggtgtat accgtttaaa aacggtggga actgttttta
    28801 cccatatccg atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc
    28861 acgatggaag caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc
    28921 aaggcagaca gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc
    28981 gttggtttac gccgtcgcgg atggtatgag gatgatgtgt tggcctggca gaaagaacgc
    29041 gaacaggaag cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa
    29101 aagcggcccc gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat
    29161 atgatcccaa acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc
    29221 agtctggttc agttcgttgc catacctgca atgagcgctt ttccctatac tctttaagga
    29281 attgctcaag ggcaaaagca catggtgcga atctttctga ttcatgctct atctttctgc
    29341 gccgtctttt ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt
    29401 cctgcatagc aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg
    29461 gttctatgcc gcgccgcttt agttcagtgc gccccaactc tttaagccag ttggctaggc
    29521 ttatgccgtc gctctgtgct tctttgtcga attgctcttt tagctctgga gaaattcgca
    29581 ttctgaattg tggggattgc ccgtcccctt tagggctttt atcgcgtttg attattgaca
    29641 agtggtcacc tattgaatta gcctttccat tgttaggtgg ccaccttaac acaagagcac
    29701 ttaaaaaagc aaagccccgc aagtgtcatt accactcgca gggcttctaa ccaacaacgt
    29761 aaactaggag ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg
    29821 gcctttgtgt ggcgttttct ggccctgagt gccggagaat ctcaaatcat ccacgtaacc
    29881 gcctggacgg aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc
    29941 gcccgtattc gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc
    30001 cgcactcact acgctgatgc acgcgcttat tgatattgaa tgcactgcag agcttgcgca
    30061 gggagaagaa cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac
    30121 gcggtcgctg aatgacgcta aaaatattct tgttgctgat tgtgaaaatg gggggggtta
    30181 tgcgtgatga tcgttttaat tccctgaaac aggaatttcc cggcgttcct gatgatgcgg
    30241 ctgatgcgct ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga
    30301 gagaatataa atcaacgggg cttgatgtac tgaatatcgc cgccgattat gcggaatatg
    30361 tggcagaggc gcgttacaga agaaaatttc ctgaggatgt aagccatgcg tgatatttac
    30421 cacgaaacaa tagaccgcgc atttcttgca ctttctcaca gtgaaaacat gctggaaata
    30481 ttgcgcatat ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt
    30541 gccacggcat taataacgct tcttgagcct gtaataatgg aactgcaaga aatagatcta
    30601 ttgcacgaca gatataaaga acagcacacc ggagaataaa aataatgaaa cttaaatatt
    30661 ctggcttaac tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg
    30721 accagtacgg cggcacggta atgattaagg gcgtggaaga acggcgtgta acctaccgcc
    30781 gtgaaggtta cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt gatttttctc
    30841 tggtacagac cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc
    30901 agaagctgaa aaccatgatt aacggattca ggggcaagaa atgaaaagcg caccgaactt
    30961 aaaaaaacag ccttacgaca agatgaccga agtcattatt tttgcgggta gtgatgcctg
    31021 ggcacatgcg aaacagtggc aggaacagga cgggcgactg gctggcgata atgtgcctcc
    31081 cgttgtgctg gctgatgatc aactggatga actggcagac ctgagaatca tcgacgaggg
    31141 gcgctattgt gtccggctgt acaaggcagg ccacatcagg ccatcaaata ttaatgccat
    31201 tgcgcacaag ctggcggcgg cgggtgtaac tgatgcgaat tattaccccg aagggatgca
    31261 cagccatatg cgggagaact ggcgcgaata cctggaacgg gtgcgcggga aagagccggt
    31321 ggaagaaaaa aaccaccagc gaaaaaccac gctaccgatg agcgttggat ctaccggata
    31381 cgacacgcaa ctggattacg tggttaaggg gattattccg gcggtatcgc tatgcagcat
    31441 atacggggct agcgggtcct ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc
    31501 cactggtcgc cagtggggag gccgcagggt tgcacatggt gcggttctct atgtggttgg
    31561 tgaaggcggt atcggcgttc cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca
    31621 ggtgaaaaat ctgtatctgg taaaccgccc catctttccg gctgccccgc ttgatgttga
    31681 tgaaatggtt atcgctgccc gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat
    31741 tattctggat acgctggcgc gttgctttgg tgggaatgat gaaaatgatt cccgtgatat
    31801 gggggcgttt atccgtggtt gtgacgaact gaaacgacgc acaggggcca cggtgctggt
    31861 ggttcaccat tccggcaagg atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc
    31921 ttcgctggat gctgaatacc ggatacgcag ggaggacgca ggaagcgaag cgctggttat
    31981 ctcatgcacc aaaatgaagg acgcggagga actcaaagaa gccgcatatg acttacgcgt
    32041 ggtggagctt tttaccgacg ctgacggtga attaatcacg tcgctggtgg tggtggatga
    32101 tccgcgccct cctgttgaac tggagcgcat cgaggaggca gggaacaaga cggaaaacca
    32161 taccgcgcta tgggggtgca tccgttcacg cacacagaac ggcgacaagt gcacgatccc
    32221 gctgttacgt gatgacatga aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct
    32281 gtacaagctg gaaaaagatg gggttattag tatcgatggg gatgatgtag cgccgctata
    32341 aaagtgagga gcaaaagcga gggggataga aagagggcca aaattagccc gctctccctc
    32401 acttttcgac ctgtatacat cctcaaaagt gaggggtaaa aaatacttat aaaacacaca
    32461 catagaaaaa ccgaaaatcc caactgcgac gaagtgagac gcttgaaaaa gtgaggcgaa
    32521 aaagtgagag gttgcgagaa atgacccaaa aacgcagaga cagaacagag ccaaaatata
    32581 aagcgttaga catgactgag cacaccttaa aggtggcaat cagaacgata gaccgccaca
    32641 cgcgggaagg atacgcgaag gaacatcccg acctgataag cgcattcatg accacggcgg
    32701 cggcaaactt tgccacgctg acagaacggg agattgccga agcggaacag gtaacaacca
    32761 tcaacgttaa aaccggagag gtggaattat gacggcacag atagccgctt acgggcggct
    32821 ggtggacgac ccgcaggtaa aacagaccag caagggcaca ccgatgacgc tggcgcgtat
    32881 ggcggtatct ttgccatgca gccaggcaca ggacggacag gcgacgttat ggttatcggt
    32941 catcgcattt ggcaaacagg ccgacttcct ggctaaacat caaaaaggcg atgttgccag
    33001 cgtatccggc acgatgcagg taagccagtg gaccggacag aacggagaaa cgcggcaggg
    33061 ctggcaggtt atcgcagaca gcgtaatcag tgcccgcgcg gcacgtccag gcgggaacag
    33121 acgcaaaacc acaggcacac agggtaatca gccaccagcg ggaggcgatg acccttacgg
    33181 tgatgatatt tcgttctgag ggggtgacga tggtacatga ccgcatagcg gaggaactgg
    33241 aggcgaaagg cttttaccgg agggcggcgg cgcgatgggg tgaagtcatg cagctggtgg
    33301 agacagacaa ggaacggcat cacatcacga tgcgacggct ggaatgttca aggaaggcac
    33361 agagggcacc ggagccgccg gataatttcg gagacctgaa aaaggcagtc gatcgcactt
    33421 atgccgaaat gggtatagat ggtgctggtg atgaaatatg gcgcaattac caggacagct
    33481 aatcaaacag ccggagaaat ccggcttttt tgcgcccaaa aaaagcccga taaggtcaga
    33541 gggttcttat cgggcttttg catatgaggt ttttggatgc actggcgttc gtgatcggga
    33601 taatcatttc ataatttgca acataactca atattattgc ataaaatgca attctgatta
    33661 taatcaggac tggataaaca tccagttatg atttttttag tcgaagagga atttcttact
    33721 atggctgaag agaaaaaagg cggtgtttcg gtgtacataa gccccgaaat cgtggaggtg
    33781 ctcaagcagc gccacaaaaa aaactatgag gctggcgtgg cggctggact ggatccgctg
    33841 atgacgccgg agccgtcgat aggttcactt gtacgctctt atttacttgc ggcgcttggg
    33901 atgcataaaa attatggggg tgaataatgg caggcaaagc aacggcactt aacactaacc
    33961 agctttttat gtacctgaat cgcggggata ttgcggattt taaattcagc cctctgttta
    34021 ccacgctgtt tttcccgaac gtggcgacat tcagcacgca aaacatcatg ctggataccc
    34081 tggacattga agaagtcacc atgtcggcgt tttgttcgcc tatggtgggt agccaggttc
    34141 agcgcgataa agggtacgaa accagcacaa tcaaacctgg ctacatgaag ccaaagcacg
    34201 aaatcgatcc aacgaaaacc atcatgcgca tggctggaga agatccggca cagcttaacg
    34261 accctaccta tcgccgtatg cgcctgatta ctggcaacat gcgccgccag ataaacgcca
    34321 ttaaggcacg cgtggaatgg ctggcggtga atgcggtaac gaccggaaaa aacatcattg
    34381 agggcgaagg catagaacgc tatgaaatcg actggaagat accggaaaaa aacatcatag
    34441 agcagtccga cggcaaaaaa tggtccgagc atgataaaga tatttacgat ccaatctatg
    34501 acatcgaact atacgcagat caggcaggtt gccccgccaa cgtcatgatt atgggcgctg
    34561 aggtatggcg cacgttacgc agctttaaaa aattccgtga actgtacgat ctttcccgtg
    34621 gttcagaatc cgccgcagaa atggcctgta aaaacctagg cgaagtggtg agctttaaag
    34681 gctatctggg cgatattgct cttattgtct attccggcaa atacactgac agcgacggca
    34741 ccgaaaaata tttccttgag cctgatttgc tggtcctggg caacaccaac aataaagggc
    34801 tggtggccta tggtgcgatt atggatcagg aagcggtaag aacgggcgca acgcaaaaca
    34861 tgtactaccc gaaaaactgg attgaggacg gcgatccggc gattgagtac gtgcagacac
    34921 acagcgcacc gcagccggtt ccggcagata ttcgcaaatt tgttaccgtc aaaattggtt
    34981 aacgggggat tatatggaca ctccatatat tgagttattt gcaggcagtc agcaggtatc
    35041 aacgacactg gtacattttg ccgctgatgc tggcgttatt caggaattta ccccgctgat
    35101 gctggtggac aatggcgagt ttaagccgtg ggatggtcag gaatctggca aggctgttta
    35161 tctgacttcg tacctcgtgg acacgtcaaa gcagaaatca gcacagtgtt acaagacggg
    35221 gatattcaat attgccgccg ttaactggcc tgagagcgtc gacaccgatg cgaaaaaatg
    35281 cgccgccttt gcgggttctg gcgtatccgt tcagccgctg gcccgataag cagggggaac
    35341 gatggcaacg aatgaaagca tcatggcgct accgctggcg agtaaattta aagccgaagc
    35401 gcgggcaatg gctgacagag gtttatcaac ctacgaggcc gtatatcaac tcaacaaact
    35461 ggaagagcag gacaagccgc gcgctgatgc gattatggcg cttcatgagc ataaagacta
    35521 tcagccgctg ttgcgtgcaa tggcaaacgt gccttgtatc gatgttgata cggctaaaaa
    35581 catcctgaac atgaccatag agcaggaacg cccgaaggtt gcaccagagc ttaccgcagc
    35641 ctttgaaaac tttatggaca tgcacagccc acaaaccgta tcagctggca tggcatacga
    35701 tggcagaaac ccaggcgatg acggcgacat cgatcgcata ctgaaaacca tctgagacaa
    35761 ggccggagaa atccggcttt tttgcgggtc cttcctggaa ttatggcccg ttacggggcg
    35821 gcgacctcgc gggttttcgc tatttatgac gtttttccgt gaaggtgaca ccaccaccac
    35881 ttgattaata tttaaccatg cagttaaggt aacattatga ttgataaagc ttgttttgta
    35941 agtcagcagg aaatagctga acatttcaag gttaacagaa ccactattcg cgcatggacc
    36001 aaacagggga tgccgtatct taatgcggat cgcggaaagt ctggcggtta tcacatcggg
    36061 catacattgc tttggtcttc aggtaaaagc cgtcttgagg ccatcagata tcacgtagaa
    36121 accagtgcgc tggaaaaaat tatgtttgcc aggctgcttt catctgagcg tgacgagtac
    36181 tccagtgaag aaacagaaca tcgatttgat gaaggtttgc agatttacgg ctattcaccg
    36241 gaagatgtga gcaaggcacg aaataaaatg gctggctttc tggctgggtg gcgtcatgcc
    36301 gtaagcgttc gccgtgccag catggagcaa tcagccgata cagaacagta aagccgataa
    36361 cttgttaatc actcgcagca atgcatgtga tttgtgcaca ctttcggtta actggttcga
    36421 tgtccggttt tagtgtctgt tttttgcgca tgtccggttc atggaaagca tgtttttata
    36481 tttttcatat ggttaacttg tagagaaacc ggacatggat cccgaaaaat tttcataaat
    36541 agtgaaaacg cgcgaggtcg ccgccccgta acgggtccat aattccagga aggacccgac
    36601 gacaccagac aatcagaacg ataggtgcac aatgacagaa gccgaaatac tgggattaat
    36661 ccgccgtgct ggtggaatca gccagcagac tgacgaacag gccacgcagc cggacagcgt
    36721 gacagccgaa aattatgcgc gtgttgttgc tgaggtgatg cgccgtgatg gtatccagct
    36781 taatgatgtg gatatgcgca acatacggat ccgcgttctt gaaatgctgg cctacaatcg
    36841 ccgcgtggca ctgtatcggg aaacggagaa aattacatac cactggaaga agccggagcg
    36901 gttgcggcgg taactggttg atattttcga aattcgccag cggcgaacat cggggaaacc
    36961 agcctaaccc gttgatattt tcgggaacct caaattgagg aggtcggaaa cataacccgt
    37021 catattctgg atgctgataa ttaccacgca gcccattaag gcctaacccg ttgattcttt
    37081 cggaaacagc cattggcgta atccggcgcg gtaacttgct gaacttaaag caaagcgcaa
    37141 aattgcgctg gctggtgggc atgatgccga tatggtgatc cccataacgg aaatatccac
    37201 cagcacggca acggcagaat agccacaaca cagaaaaacc acgaatatgg ggtttttgtt
    37261 atggcatggg catgatgacc gcccctgctc tgaatggtgg aaatcaccac gcagatcatc
    37321 caccagcttc gtttttataa attaaacatc cgggaaaatc cgaccctgaa ttgtgcattt
    37381 tttcagcaaa tcattttagt tatacgtttg gttatacggt ttaaaagttg aatcatatac
    37441 attattttaa atcatgaagt tacacgctca attaaactac tctcgtgcaa aaatcccaag
    37501 aaaaaaccaa cctttacggt tggttttttt atatctgcaa ttaatttgat aaacagaccg
    37561 tgacacatca cagcctgttt attttctgtt atcagaacgt ccagaccact cccgcctgag
    37621 tattccacgg cgattctacc cctgcgccat tgccatagct gactgacaaa tgaccgctta
    37681 acgtcggggt aaacgatgac cttatcccag cctgataaac gccaggggtt ccagccacat
    37741 cattattaaa tttgccatcg tcattaactt tcacatggtt agaatcggcg tattcctgac
    37801 gtacggcggc tttcagccag ggttccagcg tcgtaccatt ttgcaggtcc atgtgatagc
    37861 ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt tcccacatca gcgcgcatgc
    37921 cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc
    37981 taacactcca caatccgtca acccaacgga acccgctctc aacatgagca cccgcgccgt
    38041 tactattgta atcgccaaac gctgttgccc cattactcat cttgccatgg atggtgttgg
    38101 caaaacggtc aactttcacc accccatcaa cataggcacc gttctgatgc tcccaaccgg
    38161 cataagcccc cagggtatag ctatcgatat taccttttcc gccgcgatca aaaccaatat
    38221 cagaatgaga gtaaccaaag aacaagccgc gaattgtact gctttcttca cgggagaaac
    38281 ggctatcgat accgagcgtc aggcccgtca atgtttgctc aaaaccagct cccgcatcag
    38341 tggtcacgtt gttgcgggtg ttaattgccg aactccacat cgccgtatcg taactaacgc
    38401 tttttacgct accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt
    38461 gtgcggccgc catattcagc acatcagtgg ttgaaggggt aatttgcgcg cgattctctg
    38521 ccaaactcca gctatggttg ccattatcca gcaaggtata ttcatacgta ccgatatcaa
    38581 caacgcctcc ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg
    38641 taaggctatc tcctgctgcc gggctggcac cggtgtccgt cacgaatatt ttgaaatcac
    38701 ctgttgcctg accggtgacg ttgagctgat cgctctgatg attagccata tcggtacgca
    38761 tataaaaatt gccgtttccc gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac
    38821 ttgccgtcac tgatgaacga gtcactggct catccatcat aatcacattt gcgtcagaca
    38881 tcgcaaggtt ggctaagttg aggagggtgt tttggttcgt cgtagtgata tcggcaatga
    38941 tggcccatac gctattttcg agcgaaacgt ccgctttgct ggtatccacg gcaccatgcg
    39001 ttctaaaaga agcgccctca gaaaccacta ccttacccac caacatagcg ggtgcataat
    39061 ttatttgcgg cttcatgacc tcaagaatgc catcattccc tctgactgac acgttaacca
    39121 ttgtgccagc ccagacatta gcgcggccat tgttgataac catattttca gagatggctt
    39181 tagaggaata cgtgatactt ccattctggt ctgatcgacc gagatcatat accgcatttg
    39241 cctgcacttt cgtaccagta tcctttccca gcgattgcat agtggcatgc tcatcaagga
    39301 tagtatcaat ggcctgcgtg tcctccataa caatgagccc ggaaccatca tccagttcat
    39361 aattttttga cacaccatct tttatactaa attggccttt actgtttgta ccactcactt
    39421 ccagcgcatt cgttgagaca attaattttc cgcctgcttt tttatcaacg ccagttgcag
    39481 tcccgccatc cataacctcc agtaagccgc cactatctac agtagtatta taagcgaagt
    39541 cattttcttc aactcgcaaa cttccgccgt tttccaacag catattgtta gcaataccat
    39601 tcttgatttc gaactgaccg agacggtttg ttccgaatgc ctccatgacc cgcgtgcttg
    39661 ctttaatagc accgcctgct ttctgatcta ccgcaatcgc taatcccccc tctttaacct
    39721 ccagtaatcc tcccttttcg atagcggcaa ggacggtaat cccatctttt tcaacgatta
    39781 agcgcccgcc tttaatcgtg ggatcattcg ctgcgccata aacctgtagc acaccattat
    39841 tgaggg
//
LOCUS       DAJTYD010000016        39490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTYD010000016 DAJTYD010000000
VERSION     DAJTYD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.016"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..352
                     /locus_tag="OO585_000762"
     CDS             <1..352
                     /locus_tag="OO585_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6628443.1"
                     /translation="GIIRLDAFKTSKEIQFAVQSGPMLLENGVINPRIHPNVASRKIR
                     NGVGINKQGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLDGTISHMYMKGGAIPWQ
                     RYPFVTMISVERKG"
     gene            435..1337
                     /locus_tag="OO585_000763"
     CDS             435..1337
                     /locus_tag="OO585_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6628444.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1382..3544)
                     /gene="uvrD"
                     /locus_tag="OO585_000764"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1382..3544)
                     /gene="uvrD"
                     /locus_tag="OO585_000764"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6628445.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3628..4344)
                     /gene="yigB"
                     /locus_tag="OO585_000765"
     CDS             complement(3628..4344)
                     /gene="yigB"
                     /locus_tag="OO585_000765"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6628446.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4344..5240)
                     /gene="xerC"
                     /locus_tag="OO585_000766"
     CDS             complement(4344..5240)
                     /gene="xerC"
                     /locus_tag="OO585_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6628447.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5237..5944)
                     /locus_tag="OO585_000767"
     CDS             complement(5237..5944)
                     /locus_tag="OO585_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6628448.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5941..6765)
                     /gene="dapF"
                     /locus_tag="OO585_000768"
     CDS             complement(5941..6765)
                     /gene="dapF"
                     /locus_tag="OO585_000768"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6628449.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6810..7004)
                     /locus_tag="OO585_000769"
     CDS             complement(6810..7004)
                     /locus_tag="OO585_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6628450.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            7467..7787
                     /gene="cyaY"
                     /locus_tag="OO585_000770"
     CDS             7467..7787
                     /gene="cyaY"
                     /locus_tag="OO585_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6628451.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7827..10373)
                     /gene="cyaA"
                     /locus_tag="OO585_000771"
     CDS             complement(7827..10373)
                     /gene="cyaA"
                     /locus_tag="OO585_000771"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6628452.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLCGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10760..11701
                     /gene="hemC"
                     /locus_tag="OO585_000772"
     CDS             10760..11701
                     /gene="hemC"
                     /locus_tag="OO585_000772"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6628453.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11698..12438
                     /gene="hemD"
                     /locus_tag="OO585_000773"
     CDS             11698..12438
                     /gene="hemD"
                     /locus_tag="OO585_000773"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6628454.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12460..13689
                     /gene="hemX"
                     /locus_tag="OO585_000774"
     CDS             12460..13689
                     /gene="hemX"
                     /locus_tag="OO585_000774"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6628455.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            13692..14888
                     /gene="hemY"
                     /locus_tag="OO585_000775"
     CDS             13692..14888
                     /gene="hemY"
                     /locus_tag="OO585_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6628456.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15567..15728
                     /locus_tag="OO585_000776"
     CDS             15567..15728
                     /locus_tag="OO585_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628457.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15812..17047)
                     /locus_tag="OO585_000777"
     CDS             complement(15812..17047)
                     /locus_tag="OO585_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6628458.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17194..17270)
                     /locus_tag="OO585_000778"
     tRNA            complement(17194..17270)
                     /locus_tag="OO585_000778"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17234..17236),aa:Pro,seq:tgg)
     gene            complement(17313..17399)
                     /locus_tag="OO585_000779"
     tRNA            complement(17313..17399)
                     /locus_tag="OO585_000779"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17363..17365),aa:Leu,seq:cag)
     gene            complement(17420..17495)
                     /locus_tag="OO585_000780"
     tRNA            complement(17420..17495)
                     /locus_tag="OO585_000780"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17460..17462),aa:His,seq:gtg)
     gene            complement(17554..17630)
                     /locus_tag="OO585_000781"
     tRNA            complement(17554..17630)
                     /locus_tag="OO585_000781"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17594..17596),aa:Arg,seq:ccg)
     gene            complement(17733..19118)
                     /locus_tag="OO585_000782"
     CDS             complement(17733..19118)
                     /locus_tag="OO585_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6628459.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSSGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19309..20049)
                     /gene="rffM"
                     /locus_tag="OO585_000783"
     CDS             complement(19309..20049)
                     /gene="rffM"
                     /locus_tag="OO585_000783"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6628460.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20052..21404)
                     /gene="wzyE"
                     /locus_tag="OO585_000784"
     CDS             complement(20052..21404)
                     /gene="wzyE"
                     /locus_tag="OO585_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6628461.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21401..22480)
                     /gene="rffT"
                     /locus_tag="OO585_000785"
                     /gene_synonym="wecF"
     CDS             complement(21401..22480)
                     /gene="rffT"
                     /locus_tag="OO585_000785"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6628462.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(22477..23727)
                     /gene="wzxE"
                     /locus_tag="OO585_000786"
     CDS             complement(22477..23727)
                     /gene="wzxE"
                     /locus_tag="OO585_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6628463.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23729..24859)
                     /gene="rffA"
                     /locus_tag="OO585_000787"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23729..24859)
                     /gene="rffA"
                     /locus_tag="OO585_000787"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6628464.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24864..25538)
                     /gene="rffC"
                     /locus_tag="OO585_000788"
                     /gene_synonym="wecD"
     CDS             complement(24864..25538)
                     /gene="rffC"
                     /locus_tag="OO585_000788"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6628465.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25516..26397)
                     /gene="rfbA"
                     /locus_tag="OO585_000789"
     CDS             complement(25516..26397)
                     /gene="rfbA"
                     /locus_tag="OO585_000789"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6628466.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26416..27483)
                     /gene="rffG"
                     /locus_tag="OO585_000790"
     CDS             complement(26416..27483)
                     /gene="rffG"
                     /locus_tag="OO585_000790"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6628467.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27483..28745)
                     /gene="wecC"
                     /locus_tag="OO585_000791"
     CDS             complement(27483..28745)
                     /gene="wecC"
                     /locus_tag="OO585_000791"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6628468.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28742..29872)
                     /gene="wecB"
                     /locus_tag="OO585_000792"
     CDS             complement(28742..29872)
                     /gene="wecB"
                     /locus_tag="OO585_000792"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6628469.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(29928..30974)
                     /gene="wzzE"
                     /locus_tag="OO585_000793"
     CDS             complement(29928..30974)
                     /gene="wzzE"
                     /locus_tag="OO585_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6628470.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(30986..32089)
                     /gene="wecA"
                     /locus_tag="OO585_000794"
     CDS             complement(30986..32089)
                     /gene="wecA"
                     /locus_tag="OO585_000794"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6628471.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32329..33588)
                     /gene="rho"
                     /locus_tag="OO585_000795"
     CDS             complement(32329..33588)
                     /gene="rho"
                     /locus_tag="OO585_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6628472.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33673..33774)
                     /gene="rhoL"
                     /locus_tag="OO585_000796"
     CDS             complement(33673..33774)
                     /gene="rhoL"
                     /locus_tag="OO585_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6628473.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33915..34244)
                     /gene="trxA"
                     /locus_tag="OO585_000797"
     CDS             complement(33915..34244)
                     /gene="trxA"
                     /locus_tag="OO585_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6628474.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34375..35640
                     /gene="rhlB"
                     /locus_tag="OO585_000798"
     CDS             34375..35640
                     /gene="rhlB"
                     /locus_tag="OO585_000798"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6628475.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEILEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35774..37258
                     /gene="gppA"
                     /locus_tag="OO585_000799"
     CDS             35774..37258
                     /gene="gppA"
                     /locus_tag="OO585_000799"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6628476.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37305..39326)
                     /gene="rep"
                     /locus_tag="OO585_000800"
     CDS             complement(37305..39326)
                     /gene="rep"
                     /locus_tag="OO585_000800"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6628477.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9797 a        10758 c         9984 g         8951 t
ORIGIN      
        1 cggcatcatt cgtctggatg cctttaaaac cagtaaagag attcagtttg cggtgcagtc
       61 agggccaatg ttattagaaa acggcgtaat taatccacgc attcacccga atgttgcctc
      121 gcgtaaaatt cgtaacggtg tagggattaa taaacaaggg aacgccgtgt ttttgttgag
      181 ccagcaggcg acaaattttt atgattttgc ctgttatgcc aaagcgaaac tgaacgttga
      241 gcagttgctt tatcttgatg gcacaatttc acatatgtat atgaaaggcg gggcaatccc
      301 gtggcaacgc tatccttttg tcaccatgat ttccgtcgag cggaaaggtt aagcattgta
      361 cactcgagga ggccagacaa actgggactg gctacacagc tggaaaatca tcagataaat
      421 gaagggaaac aaaaatgaaa aaaacgctcg caacgttatt tttattaacg tgtctgggat
      481 catcatccgc ctatgcggat aacgctttga ttttacaaac tgattttagc ctgaaagatg
      541 gtgccgtctc ggcgatgaaa ggggtcgcat tcggcgttga ccataatctt aaaatctttg
      601 atttaacaca cgaaattcca ccgtataaca tctgggaagg cgcttatcgc ctgtatcaga
      661 ctgccagtta ctggccgcaa ggttcggtgt ttgttagcgt ggtcgatccg ggcgtaggca
      721 ctgaccgtaa atcggtagtg ctgaaaacca aaagcggtca atacttcgtt tcaccagata
      781 acggtacgct cactttggtg gcacaaactc tgggtattga tagcgtccgg gaaatcgacg
      841 aaaaagctaa ccgcctgaaa ggttctgaaa agtcatatac cttccacggt cgtgacgtgt
      901 atgcctacac cggtgcacgt ctggcttccg gggcgattac gttcgaacag gtggggccag
      961 agcttccagc aaaagttgtc gaactttctt atcagaaagc aaaagcaaca aaaggtgaag
     1021 tgaagggcaa tatcccgatt ctcgatattc agtacggcaa cgtctggagc aatatcagcg
     1081 acgagttgct gaatcaggca gaaatcaaac tcaacgacac gttgtgtgtg accatttccg
     1141 aaggttccca gcaaaaatac gcagggaaaa tgccgtatgt cgcaagcttc ggcgatgtgc
     1201 cggaaggcca gccgatggtg tatctgaaca gcttattgaa tgtttccgtg gcgctgaata
     1261 tggataattt cgcgcagaaa catcaggttg cgtctggtgc tgactggaat attgatgtga
     1321 agaagtgcgc taagtaaggt gctgatgcgg aatgcggtgt ggcgcaccgc atcccgcaac
     1381 gtcacaccgt ctccaaccgg gcataagccg ccaccagcca tttaataccc tggccctgga
     1441 atgccacctg caaacggcta tgttcaccgc tgccttccat attgacgatg gtgccttcac
     1501 caaacttagc gtggcgtaca cgctggccga gcttgtagcc gctgtcgttc tcgaccatcg
     1561 gcgtacccat ccgctgatgg ctgaccgggc ggcttaccgt ggcgcgcagg cgcacctctt
     1621 ccacacactc ttccggcagc tcgccaataa agcgcgacgg gcgatggtaa acctctttgc
     1681 catacagacg gcgagtttcc gcgtaggtca gcgtcagttt ctgcatcgcg cgggttacgc
     1741 caacgtaggc cagacggcgt tcttcttcca gacgcccgcc ttcatccagc gacatctggc
     1801 ttgggaacat gccctcttcc ataccgacga taaacacctg cgggaactcc agacctttcg
     1861 ccgagtgcat ggtcatcagt tgtaccgcat cctgccaggt atccgcctgc ccttcgcctg
     1921 cttccagcgc cgcatgggat aggaacgcct gcagcggcat taaatcttcg tcttcttcgt
     1981 tgtagctgaa ctggcgcgtt gccgtcacca gttcctctaa gttttcgata cgcgtctgac
     2041 ctttttcgcc cttctcctgc tcgtacatgg tacgcaggcc ggagtcttta attacccggt
     2101 cagtctgtac atgcagcggc atatcggcag tttcctgcgc taaggcgtcg atcagttcca
     2161 taaaccgctg taaggcgctg gcagcacgtc cggcgagggc tttttcctgc aacagttcac
     2221 gacatgcctg ccagagtgtt aactggcgat cgcgcgatgt ctgacgtacc acgtccagcg
     2281 tccggtcacc aataccccgc gttggcgtat tcaccacacg ctcaaaggcc gcgtcgtcgt
     2341 tacggttggc aatcaggcgc agatacgaga gcgcatcttt gatttcctgg cgttcgaaga
     2401 agcgcatccc gccgtaaata cggtacggca tactcgcctg caacagtgct tcttccagca
     2461 cacgcgactg ggcgttactg cggtagagaa tggcgcactc ggcaagcgcc ccgccgttgt
     2521 cctgccaggt tttgatgcgg ttaaccacaa aacgcgcttc atcgagttcg ttaaaagcgc
     2581 aatagaggga aataggctca ccgtccgcgc catcggtcca cagtttttta cccagacgcc
     2641 cgttattgtt ttcaatcagg gcgttagcgg cgctcagaat attgctggta gagcggtagt
     2701 tttgctccag acgaatagtt tcggcaccgg ggaaatcatt aaggaaacgc tgaatattct
     2761 ccacctgcgc cccgcgccag ccgtagattg actggtcgtc atcaccgacg atcatcactt
     2821 tgccggtgtc gcccgccagc aggcggatcc acgcgtactg aatgttgttg gtatcctgga
     2881 attcgtccac caggatattg gtaaaacgtt cgcggtagtg ttgcaggata tgcggcttgt
     2941 taagccacaa ctcgtgagcg cgcagcagca gctcggcgaa gtccaccagg cccgcgcggt
     3001 cacacgcttc ctgatacgcc tgatacacct tctgccaggt ctgctccacc ggattaccgt
     3061 agctttgaat atgatgtgga cgcaggcctt catctttctg gctgttgatg taccacattg
     3121 cctggcgcgg cggccactgc ttctcgtcga ggttcatcgc cttgattagg cgtttgagca
     3181 ggcgtagctg gtcctcgctg tcgaggatct gaaaatcctg cggcagattg gcgtccatat
     3241 ggtgcgcacg cagcagacgg tgcgccagcc cgtggaaggt gccgacccac atgccgccct
     3301 ggctggtgcc cattagctgc ccgatacgat gacgcatctc cgccgccgct ttgttggtaa
     3361 acgtcaccgc cataatcgag tatggcaagc agttttccac gctcatcagc caggcgatac
     3421 gatgcaccag tacgcgcgtc ttaccactgc ccgcgcccgc cagcaccaga aggttgctgc
     3481 gtggcgcggc caccgcttcg cgctgtttgt cattaaggct gtcgagcagg taagaaacgt
     3541 ccattggcac cgccgcgtaa aaataggttg ggcggagaag cgcaacgccc tccgccaaaa
     3601 agctgggtat atatacagat ttgctgatta tatcagcgag gtcagagatg ccaaccggga
     3661 aatttccaga tgcggcagta aacggctgtc ccaggtttgc atcagatcgc cattttccgg
     3721 tctgatccaa caggcctgca ttccgctgcg aattgcccca cccacgtcag tggtgagatc
     3781 gtccccaaca tgtaagatct cgccgatcgg cacgttgagt ttttccgcag ccagaaagta
     3841 catatcgctg aacggttttg agcgcccgtg cgggccagcg cgcagcacaa actcaaaata
     3901 atcccccaaa ccaaacagtt ccggctgggc gttaccgttg gtgatcgcca ccagcggcca
     3961 tttcttcgcc agctgtttta aggtgtcgtg agtttgctgc gggacgtcga ttcggctgcg
     4021 ccatttggca aagttgatca ttgctgcgtg tgcgcctgca ctggcttctt cggcactcag
     4081 cccggcgtcg agcatcgctt gttcaatcga acgaaaacgc cagcgcgtca cgtcgtgata
     4141 aatctcgggt tccgcttccc gtaccgcctg gcgcaggcgt tgcagatctt cattctggaa
     4201 gctgcgcagc gccggatgat aattttgcac aaaggtaagc gcctctcgct cggtgcgcaa
     4261 aatcaccgga cggttatcgt aaagggtatc atccaggtca aaggtgagcg ccgagatgcg
     4321 ccccaaaggc cggtaaaaac gcattatttc ccccgtttgg cgcgtggatg cgccgcatcg
     4381 tacaccgagg caaggtgttg aaaatcaaga tgagtataga tttgcgtggt ggagaggttg
     4441 gcatgaccca gtagctcctg cacaccacga agatcgccgc tcgactccag catatgcgta
     4501 gcgaacgagt gacgtaattt atgtgggtga acgtgattat tcagcccttg ttttatgccc
     4561 cattcggcaa agcgtttctg cacattacgc gcggagatac gcttgcccag tttcgacaga
     4621 aaaagcgcgt cgtcttcgct accaaacagg tcgcgcaaat caagccagtg ctcaatccac
     4681 gccacagcgt tgcgaccaat cggcaggcgg cgctctttgc tgcctttccc cattacccac
     4741 acttcaccag actccaggtc gaggtgtttg atatccagcc ccaccagctc ggaaagacgc
     4801 agaccagcgc cgtacatcac ttccagcatt gcacggtcgc gtacagcgag gggatcgttg
     4861 atatcaatat ccagcagccg attcatatcg tcgacgtcga tatttttcgg cagatgacgc
     4921 ggcgctttcg gtgccgaaac acctttcgcc gggttagctt tgagttcgtt ctggctgacc
     4981 agccagtcaa aaaagctacg tagcgcagaa agccgtaacg ccagacttgc tgctcccagc
     5041 cctttacggc gactgcgtac agcaaaattg cgcaccatcg tcacatcaca ttgctgccag
     5101 ctttgcaggc cgttttcgct ggcaaaattg atgatcgcct caagctgacg ctggtagtta
     5161 agcagggtta tcgggctaag ctggcgctcc acgctcagat aacgtaggta gcgttctaca
     5221 tcggtgtgta aatcggtcat acgcgttcaa tccaacgctc cagaagctcc ggcaacatca
     5281 gcgcaatttc atgaagtaac tgcgttcctt gcccttgttg atagtgactg gcatcgcgac
     5341 tggtaaacag cacgacaccc aaatcagcat cgcttcccag catcgacatc gccaccgatc
     5401 ccaccgcttt cgcttccggt agcaccacca gcagctctgg tccgttaagc ggcccaagat
     5461 agtgctgttc ctgccccaaa cgctgaatac gcagcggttc gaaagactga cggcttaatg
     5521 ccagatgagt gtggttcgac ggcgcaccta agcgccagcg atccggaaac aggcgcagac
     5581 tcgcacctgc caggccgaga tcgcgcgccc agcggtgaaa gcgcatcagc atatcgtcga
     5641 gactgctggc ggcggtgaga ctgcgctgca ggtagagtag gcgataaaac aggccttcgt
     5701 tggcgatagc ctgttccatc aacagcgcca tgttctcttc cagaacatga atatgattac
     5761 gtgcgcgggc catgtgccac tcgaccaacg aaacggtgcc gcgtaccgga tgtggcacac
     5821 gtatcgcttc tactgcgcgc gcattacgga taaaaaactc aggattttta atcagataat
     5881 cgacaaccgc ccggtcatca agctccgtga gtgtttcctg cagttcttcc cctggttgct
     5941 tcatagatga ataaatccgt cgtagacatg taccgccggg ccagtcatat ataacgggtg
     6001 acccggacct ttccaggcga tatcaagacg accgccgggg agttccacgc gtacttcttc
     6061 ggccagcaaa ccttgctgaa tccctactgc aaccgccgca cacgcgccgc tgccgcaggc
     6121 ctgggtttct cctgccccac gctcataaac gcgtaaacga atatgctcgc gcttaaccac
     6181 ttgcataaaa ccgatattgg cgcgctccgg aaaacgctcg tggctttcca gaacaggacc
     6241 aagcgtttct accgccgcgg tatcgacatc atcgacctga atcacgcaat gcggatttcc
     6301 catcgacacc acgccgcata agattgtctg ctcggcggcg cgcataatat aggtcttttc
     6361 cgctttgtta gcgcgaaacg gcacagcgga aggttcgaag ttgggttcgc ccatatttac
     6421 gcggaccaga tcatcatcgg tgacggtcag aaccatccgc ccgttggcgg tgctgacgcg
     6481 gatatcgcgc ttattggtca gccctttcag gcgcacaaaa cgggcaaagc agcgcgcacc
     6541 gttgccgcac tgcgccactt cactgccatc agcattgaaa atgcgatagt gaaaatccag
     6601 ttcaggatca tacggcggct caaccaccag cagctggtca aaccctaccc ccaggtgccg
     6661 atccgccagc cgacgaatca gctccggtga aaaaaagaca ttctgcgtta ccgcgtcgac
     6721 gaccataaaa tcgttgccaa ggccatgcat tttcgagaac tgcatcattt actccaatca
     6781 cgcgggtaca gaaactgact tttagtaatt cacctgggat ggaccatccc agtggcgcga
     6841 tcgtttttat ccggcaccgt ggattgcgtt tgcgtctcta ccggtttggt cggcggcggt
     6901 gcatttttat ctgcaggcgg gaaatagagc ggacctttca gaccgcagcc cgtcaggctg
     6961 aagagagtaa gtaatacagt gagtacctta aacacgtttt tcattatgga ttgcctgtaa
     7021 agttcatcgc tttctgcttt ctatcatcgc aggagaaggc gtaaaagcaa gcgtttagcg
     7081 actctttatg aagtctgaaa agtgaggaaa tgctgcgagt ataacgctta ctcgtgagga
     7141 tttatgttct gctggcagtt tcgtgatcgg cacctcgcgc caggatggct ttatgcacta
     7201 tcgcaatgtg gtttgtttgt tggctttttt agttccgtca gcgtgaggat gctcgctcgg
     7261 taaacctgat cataatgttt accagaaaca gggaaaggcg tggtgtaact gcgccctaat
     7321 gaagagacaa atctttcgcc acacagagac atcattattc cggtgacgga tgaatcatgc
     7381 cggaacgcag cagcagaggt gcaaactttg aagccctgac cccatgccgc tatactgccg
     7441 ccatcactta aaaacaggat acaacaatga acgacagtga atttcatcgc ctggctgatc
     7501 aactgtggct gaccattgaa gagcatctgg acgactggga tggcgacagc gatatcgact
     7561 gcgaaatcaa cggcggcgtg ctgaccatta cctttgaaaa cggcagcaaa atcattatca
     7621 accgccagga accgctgcac caggtatggc tggcaaccaa acaaggcggc taccattttg
     7681 atctgaaagg cgatgagtgg atttgcgatc gcagcggcga aaccttctgg gatttgctgg
     7741 aacaggcggc gacgcagcag gcgggtgaaa cagtcagttt ccgctaagat tgcatgccgg
     7801 ataagcctcg ctttccggca cgttcatcac gaaaaatatt gctgtaatag cggcgtatcg
     7861 tgatcctgat tggcaggcgg catgttaccg atagattttg tgcggaacgg aatcacctgt
     7921 tcacgaccat caaccttcac aatctgatag aactgcggca ggttgaagtt gatgaagctt
     7981 gagccgtagg tgaagcggtc gtgcgatgac gagtagaagc gactgacgtc acgcaccagt
     8041 tcctctttgc tgccttcgca gtggtgatac acctcaaccc ggttgctttc gtcaagaatg
     8101 tagatattaa agccattctc gtcttgcgtt tcttcgaaga agaactggat gatcccttca
     8161 ctagcaaagc cgtccaccac cgccggtaat ttgacgtgat tggtttcaac ctgcactgac
     8221 aggccgtgca gtttgttatg cgaaatagcg ccataaaact cgatggcgtt ctccagcttc
     8281 tgtaccgata cattcaggcg ttcgaagaac aagccccagg tttgaccaga aacgcgcagc
     8341 gccttgaaac gcccggtttc ctggcgggtg ctggaaaggc gcagttcaat acactcagaa
     8401 accagttgct gcacgcgagt acggattaac ccgcacagat gctggctata acagaagact
     8461 tccacgctat ctggcggtgc ggcgtcctga tgcattttgc cgagaatagt tttcagggct
     8521 tcgatcatcg attgctcgcc gttgaagtgc agcgtacgca cttcgttcca cgagttgcgg
     8581 tacagcaggt caacgctacc taccaggcaa ttttgattct cgccaaagct gaagacatcc
     8641 agcttacgga aatcaaaatg caccacctga ttgcggaacg ccgctgtcgg gtcatattcc
     8701 aggttaacga taatcgccag atggcggatc tcacacgggc tgtagagcgc cttcggtgtc
     8761 ggtgcaggta agcgcagcgg gaaatggtgc gacacgtcgg cgaccatctc ctgcaactta
     8821 ggcaaatcga caatgccgtt acctttaata tacaaacggg tgcgcgaggt cagcaggccg
     8881 ttaaaccatg cccacgccac cagtttgttc aggtaacggt tatattccag cggctgatgg
     8941 ctgatgatcg actcaatatt tggcgcgcgg ttatacagat accaacctga acggttagca
     9001 cggcccggcg gcacataaat aaaggtcaga ttcggttccg agagatcggg tgaaatctgc
     9061 gggtttacca gcgtcacttt acctggtaat gcttcaaacg cggcatacag cttacgggtc
     9121 agcacgccga tatcctgcgg actggcggag acgctaaggt tattgcgacg cgcaaagcgg
     9181 atcagattac ggtagctctg catcatcgcg tcgagcaact cgttgtgcgc ctcacgcacc
     9241 tgatcaatct tccagttagc gcggttatcg agcattgcca gacgagcttc gtcccaaccc
     9301 cactcgctca ctaactggct caacactgcg cgacgccagc ctacgcaggc gcgttcacgg
     9361 ctgagctttt cgcacacttt taaatagaag cagcgacgta ctaaatccag acgggtaaaa
     9421 tcttcaatcg ccgtcaggta ttcagtaaca cgctccagca tcatgcagta tggatcgaga
     9481 ccaaacgata caatctcacc gtcgtgcaaa cgctgtttga tatctttcgc cagcagacgt
     9541 gggttcgggt attcccagga ataggcttcc agcagcagtg ttttcagtac cgctttgtat
     9601 ggggaatcga tactcttgta gagctgccaa aggctggcac caaagtactc ttcagcagaa
     9661 agcgagctta agccaccgag atccagccat tcatttggcg tcagcacgcc ctgcgcgtaa
     9721 agcgtcatca catagtcgtc gtaatgctct tcttcgtcgc acggcaccat attccacaga
     9781 atacgcttac cggcgagacg cacggcggta cgataaaatt cgtcaagcag cagtatatgc
     9841 tgggtggagc cacaatcttc gccccccaag ctgccgcttt cattatgacg gaagcggttt
     9901 tcatcaatca ggaagaagct gacttccaca cccagcgagg cggcccagct ttccagcaga
     9961 ctacatttac gttgtagtaa ctggcgctct tcgctatcga gccaggattg atgacagacc
    10021 cagatatcca ggtcagagga acaactttgc cctacggacg aggtgctgcc catggtgtat
    10081 acaccagtaa ttggaagctc acctttcggt ggatcctgta ctgacattcc acgatacagt
    10141 tcaagctcgt tcaggtagtg gcgttgagtt tcatcaggcg tgtaaaggca aatgcctttg
    10201 ggaacgttac catcaaggta acccggcatt agcggatggt gatagtgcaa caatgtcggc
    10261 agtagactgt agacctgttg gaatgcaggc cccatagcag caagcgcgcg atccacacgc
    10321 aattgattta tggcatccag tctctgtttc agagtctcaa tatagaggta caagacgtat
    10381 cgcctgattt gctacccgtc atgactgtga ttccgccaac atcaacggta acacgcggca
    10441 ttcgggatat ttcgtatgtc aaaggtaacc gttaccactt ttcgcgcctg gtttttttag
    10501 tttcacgacg aaaaaatggt ctaaaacgtg atcaatttaa caccttgctg attgaccgta
    10561 aagaaagatg cgctacatac aagtgtagca ccgtttattc tctgtaaatt ccttattacg
    10621 acggcgtgaa acgcctgtca ggatccactg ccagacctca ttttacggtt tgcgcaggcg
    10681 tctacgtttc accacaacac tgacatcact ctggcaagga tgttaggatg gaccacggat
    10741 gataatgacg gtaacaagca tgttagacaa tgttttaaga attgccacac gccaaagccc
    10801 acttgcactc tggcaggcac actatgtcaa agacaagttg atggcgagcc atccgggcct
    10861 ggtcgttgaa ctggtaccga tggtgacgcg cggcgatgtg attcttgata cgccgctggc
    10921 gaaagtaggc ggaaaaggct tatttgtaaa agagctggaa gtcgcgctcc tcgaaaatcg
    10981 cgccgatatc gccgtacatt caatgaaaga tgtgccggtt gaattcccgc aaggtctggg
    11041 actggtcact atttgtgagc gtgaagatcc tcgcgatgcc tttgtgtcca ataactatga
    11101 cagtctggat gcgttaccag caggcagtat cgtcgggacg tccagtttac gtcgccagtg
    11161 ccaactggct gaacgccgcc cggatctgat tatccgctcc ctgcgcggca acgtcggcac
    11221 tcgcctgagc aaactagata acggcgaata cgatgccatc attcttgccg tagccggact
    11281 aaaacgttta ggtctggagt cccgcattcg cgccgcgtta ccacccgaga tttctcttcc
    11341 agcggtagga caaggtgcgg tcggtattga atgccgcctt gatgatgcac gcactcgcga
    11401 gctgcttgcc gcgctgaatc accacgaaac tgcactgcgc gttaccgcag aacgcgccat
    11461 gaatacccgt ctcgaaggcg gatgtcaggt gccaattggt agctacgccg agcttattga
    11521 tggtgaaatc tggctgcgtg cgctggtcgg cgcgccggac ggttcgcaga ttattcgcgg
    11581 tgaacgccgc ggtgcgccgc aagatgccga acaaatgggg atttcgctgg cagaagagct
    11641 actgaataac ggcgcgcgcg agatcctcgc tgaagtctat aacggagacg ccccggcatg
    11701 agtatcctgg tcacccgccc gtctcccgct ggagaagagt tagtgagccg tctgcgcaca
    11761 ctggggcagg tggcctggca ttttccgctg attgagtttt ctccgggtcg acaattaccg
    11821 caacttgctg atcaactggc ggcgctgggg gagagcgatc tgttgtttgc cctctcgcaa
    11881 cacgcggttg cttttgccca atcacagctg catcagcaag atcgtaaatg gccccgacta
    11941 cctgattatt tcgccattgg acgcaccacc gcactggcgc tacataccgt aagcggacag
    12001 aagattctct acccgcagga tcgggaaatc agcgaagtct tgctacaatt acctgaatta
    12061 caaaatattg cgggcaaacg tgcgctgata ttacgtggca atggcggtcg tgagctaatt
    12121 ggggataccc tgacagcgcg cggtgctgag gtcacttttt gtgaatgtta tcaacgatgc
    12181 gcaatccatt acgatggtgc agaagaagcg atgcgctggc aatcccgcga ggtgacgatg
    12241 gtcgttgtta ccagcggtga aatgttgcag caactctggt cgctgatccc acaatggtat
    12301 cgtgagcact ggttactacg ctgtcgacta ttggtcgtca gtgagcgttt ggcgaaactc
    12361 gcccgggaac tgggctggca agacattaag gtcgccgata acgctgacaa cgatgcgctt
    12421 ttacgggcat tacaataact ctcataacag gaagccataa tgacggaaca agaaaaaacc
    12481 tccgccgtgg ttgaagagac cagggaggcc gtggacacca cgtcacaacc tgtcgcaaca
    12541 gaaaaaaaga gtaagaacaa taccgcattg attctcagcg cggtggctat cgctattgct
    12601 ctggcggcgg gcgtcggttt gtatggctgg ggtaaacaac aggccgtcaa tcagaccgcc
    12661 accagcgatg ccctggctaa ccaactgacg gcattgcaaa aagcccagga gagccaaaaa
    12721 gccgagctgg aaggcattat taagcaacaa gctgcacagc ttaagcaggc gaatcgtcag
    12781 caagaaacgc tggcaaaaca gttggatgaa gtccaacaaa aggtcgccac catttccggc
    12841 agcgatgcta aaacctggct gctggctcag gccgattttc tggtgaaact cgccggacgg
    12901 aagctgtgga gcgatcagga cgtcacgacc gctgcagcgt tgctgaaaag tgcagatgcc
    12961 agcctggcgg atatgaatga cccgagtctg attaccgttc gtcgggcaat taccgatgat
    13021 atcgccagcc tttctgcagt ttcgcaggtg gattatgacg gcatcatcct taagcttaat
    13081 cagctttcaa atcaggtaga taacctgcgt ctggccgata atgacagcga tggttcgccg
    13141 atggattccg atggtgaaga gctttccagt tccatcagcg aatggcgtat caatctgcaa
    13201 aaaagctggc agaactttat ggacaacttc attacgattc gccgtcgtga tgacaccgcc
    13261 gtaccgctgt tagcgccaaa tcaggatatc tatctgcgcg aaaatattcg ctctcgcctg
    13321 ctggtcgcag cacaagctgt accgcgtcac caggaagaga cttatcgcca ggcgctggag
    13381 aacgtctcca cctgggtacg tgcttactac gatactgatg atgccaccac caaagcgttc
    13441 ctcgacgagg tggaccagtt aagccagcaa aatatctcga tggatcttcc ggaaaccctg
    13501 caaagccagg cgatgctgga aaaactgatg cagactcgcg tgcgtaacct gctggcacaa
    13561 ccggcagcag ggacaacgga agctaaacct gcacctgcac ctgcacctgc acctgcacct
    13621 gcacctgcac ctgcacctgc acctgcaccg caaggtgata ctccggcagc cgcgccgcaa
    13681 ggagaataac catgctaaaa gtgttattac tctttgtttt gctgattgcg gggatagtgg
    13741 ttggcccgat gattgccggt catcagggtt atgtgctgat ccagaccgac aactacaata
    13801 tcgaaaccag cgtcacgggc ctggcgatca tattgattct ggcgatggta gtgctgtttg
    13861 ccattgagtg gctactgcgg cggatcttcc gcactggcgc gcacacccgt gggtggtttg
    13921 tcgggcgtaa gcgtcgccgt gcacgtaagc agaccgaaca ggcgctgctg aaactggcgg
    13981 aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa tgccgatcac gctgaacaac
    14041 cggtggtgaa ctatctactg gctgccgaag ccgcgcaaca acgtggtgat gaagcgcgcg
    14101 ccaaccaaca tctggaacgc gcagcggagc tggccggcaa tgacaccatt ccggtagaaa
    14161 tcacccgtgt acgtctgcaa ctggcccgta atgaaaacca tgctgcacgc cacggcgtgg
    14221 ataagctgct ggaagttacg ccacgccatc cggaagtatt acgtctggcg gaacaggcgt
    14281 atatccgcac aggtgcatgg agttctctgc tggatatcat cccatcaatg gcgaaagccc
    14341 atgttggtga tgaagaacat cgtgcaatgc tggaacaaca ggcatggatt ggcctgatgg
    14401 atcaggcgcg tgccgataac ggtagtgaag gtttgcgtaa ctggtggaaa aaccaaagcc
    14461 ggaaaacgcg tcatcaagta gcgttgcagg tggcaatggc ggaacatctt attgaatgtg
    14521 acgatcatga tactgcccag caaattatca tcgatggcct gaaacgccag tacgacgatc
    14581 gcctactgct gccgattcct cgactgaaaa ccaacaatcc ggaacagctg gaaaaagtgc
    14641 tgcgccagca gatcaaaaac gtcggcgatc gcccgctgtt gtggagcaca ctgggccagt
    14701 cactgatgaa gcacggagaa tggcaggaag catcgctcgc cttccgcgca gcgctgaaac
    14761 aacgtccgga cgcctacgat tacgcatggc ttgccgacgc gctggacaga ctgcacaagc
    14821 cggaagaagc tgcagcgatg cgtcgcgacg gtttgatgtt aacgttgcag aacaacccac
    14881 cacagtagtt ccttctcacc cggaggcaag cacctccggg acctttctga tgcataaaaa
    14941 aacgcctgct cttattacgg agcaggcgtt aaaacaggtc tgtatgacaa caagtgggtg
    15001 cttcactcaa cgttgtgtcc atggtgtctg atgaggcata agcgacatct gtcagtggac
    15061 gataagcacc gtaaacggct ctgcgtcatt ccggagttta tgaggcacta aggcgaacat
    15121 aagagatgga atgagcatct actcgtttat tatgccacag agaatcggga aataacatcc
    15181 cttaacactt gttatgagat aattctgtaa tcctctttgc ttcctgagta ataacttcct
    15241 gagtgaatat ttaacctgag cttgatccta cacatactta ttatgaatga taaaattcat
    15301 tcaattaata acacatatat taattgccgt taaaactaaa aaaagcatca ataatcaacg
    15361 cgatataata aacctgcctt acatatcaac tgcgccagag gtaggattga aaacgctctc
    15421 ctgattttcc aattcatttt ctggataaat aaataattta tttttgtcac tattatttat
    15481 gtaatcatcc tgtcagggag agggatctca attatcaatg cttaattacg tcatcatttt
    15541 gatgacatga aaccggagca ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg
    15601 ttgctgtcgc cgccgctctt cctctcatgg ccagcgcagc agataccccg tcaactgcca
    15661 ccgcacgcaa aggctttgcc ggatacgatc acccaaacca gtatccacag aacgctgcaa
    15721 tttattgaat ttgcacgatc atgtaggccg gataaggcgt ttacgccgca tccggcaatc
    15781 aaccgcaggc ggccacaaaa tggccgctat tttacttatt caccaccagc aaatgtgcct
    15841 gcataatgtc actggccggg cgaccgtgcg ccagcaaatc agccattgct ttaagatatg
    15901 gcggtagatg gcggaaataa cgctgatacc cggcacacaa ataattcagc cccggtttgc
    15961 cgctggcatc gagcatgaag cggtgtttcg ggcagcctcc ccagcacgct tttaacacgt
    16021 tacaactgcg acactgcgcc ggtaattgct taaatttatc ttcaccaaac acctgctgtt
    16081 gcggggaatc gatcatttct gcaattgttt gctgatgcat attccccagc cgatattgcg
    16141 gataaacata gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc accgagcgcc
    16201 cacaggttgg ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg gcaaacgccc
    16261 attcgatatt catcacgaaa atcttgccga cgtcgcgttt gatccagtgg tcaaaaatcg
    16321 ccaccagaaa ttcaccaaat tcatcggggc gcacagacca ttccgtcagt tccccctgaa
    16381 tatcaccagg cgcatgtagt ttcagtccat cgctggcagc tgtttcatca gctaggcgct
    16441 cgaccaccgg aataaactgg atgaattcga ctcccgcatc gcacaaaaaa tcataaacct
    16501 gcaacggttg ctgcgcgctg gtgcggttga cgcagaccag cacgttatag tcgacatgat
    16561 gtttttgcag cagcgtcagg gcacgcatca ccagcttatg cgttggtctg ccacctttgg
    16621 tcacgcgata ttgattgtgg atctcagccg gaccgtccag cgataaccca acaagaaaat
    16681 gattttctgc cagaaatgca caccatttat catcgagcag cacgccgtta gtctggaagc
    16741 tgttacttat cttcctgcca gcaccgtatt tcgcctgtaa ctttacggca cggcggtaaa
    16801 aatccagccc gagtaacgtt ggttcgccgc cctgccaggt aaaagccact tcgttttgcg
    16861 gttcgctggc agcgatatag tgacggacat acgcttccag cgtgtcatcg tccatatgcg
    16921 tgactggctt ttcgcggtaa agggattgtt tttcgagata aaaacagtag tcacagttca
    16981 gattacaatc ggaaccactc ggtttcgcca tcacatgaaa agcacgcgtt ggaacctgtt
    17041 gcagcatggt cactccttgc ctgaagtcat ccttcaatac gttaagggcg aaatcatacc
    17101 gtggggcaat gagttattac gggcgtttat tgcgaagaga gggtgacgaa atgcacagaa
    17161 aacaaaaaac cccgccgaag cggggttcaa aattggtcgg cgagagagga ttcgaacctc
    17221 cgacccactg gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg aaatactgct
    17281 ttttgaattt ttagttcaat tatttaaagt cgtggtgcga ggggggggac ttgaaccccc
    17341 acgtccgtaa ggacactaac acctgaagct agcgcgtcta ccaattccgc caccttcgca
    17401 ttgtcacaac ttctaataat ggggtggcta atgggattcg aacccacgac aactggaatc
    17461 acaatccagg gctctaccaa ctgagctata gccaccacta caaatcttgt tacgcggtat
    17521 tactaccacc gcagctcaag cgccgggact aaatggcgcg cccgacagga ttcgaacctg
    17581 agacctctgc ctccggaggg cagcgctcta tccagctgag ctacgggcgc ttagcgccgt
    17641 tgcgggggtg gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg
    17701 cgcgtttggt tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttcccg
    17761 tggcgattaa ggccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgcca
    17821 acaaacagcg acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg
    17881 aatgccattg ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca
    17941 atcgccgctt tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc
    18001 accatccccg gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga
    18061 atgatgtagt tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca
    18121 actggtacgc cgtgacggga aactttcgcc atcgccgccg gtaactgacg gtttttcgcc
    18181 agtgcgtaga gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc
    18241 gtcagcacca caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc
    18301 agtacgaacg ggctgccgct gctgccactt tcattccacg ggaagatggt gacgataacg
    18361 aaaatcgcgc ctacgtagaa aatcaggatc cgccacagca ctttgcctac ggcactgcgc
    18421 aacgtcacct gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg
    18481 taggacgcca ccacaataca cagcgcggtc aggaaccctt tccagccacc cgcaaagaaa
    18541 ccgccatgct ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag
    18601 aaaatcacgc ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg
    18661 aaccagaact cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc
    18721 gccactgcga tcaatgcggg tatccactgc gccatctccg ggaaccagaa ctggacataa
    18781 acgccaacgg cggtgatttc tgagatcccc accgccatcc acataaacca ataagaccag
    18841 gcggtgagat aaccaaagaa cgggctcata taacgatgcg cataaacggc gaacgaaccg
    18901 gtaaccggtt cgaggaacaa catttcgccc attgaacgca tgatgaaaaa gacgaatagc
    18961 ccggcgatga tataggccaa caatacggat ggcccggccc atttcaaggt actggcggcc
    19021 cccataaaca ggccgacgcc aatggtgccc cccagggcga tgagttcgat atgtcgagct
    19081 tccagcccac gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca
    19141 tgctttccgg tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta
    19201 attcaggaga aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc
    19261 agacaggcga cggagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc
    19321 ggtgtagtgc cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg
    19381 cgaaagcagg cggtagagcc actccagtcc cagcgtttgc cagattttcg gtgcgcgttt
    19441 tacgtgaccg gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg
    19501 caccagacgg cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac
    19561 gatttgcgct ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa
    19621 ataaccatcc tgactgccaa cgatattcac attccactgg ttgcgcagtt tagcttcagt
    19681 ttgcgccagc acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc
    19741 gcgcgccatc agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg
    19801 gtacttttta cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc
    19861 gttaattaac tccctgacct cggcgttatc ttcaatggtc agcatttttt cagcattaat
    19921 ggcaaccagc gttccctgct taagctgccc gtcagcaaac agataatcga gggcgtgctg
    19981 catatcacgc caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt
    20041 gttattcatt gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg
    20101 agtccggcgc tttcaaaaag ccagtacaac agttttgcga tcatcagaca tgcgccgaag
    20161 accacgataa aaaagactac gcgtgagaca aacgaatcca gcccttcacg tgccagcacg
    20221 atcatattga agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta
    20281 ggctcgcggt tgcccagctc atacagccag tcgaaccatt tgatgatcag accaaccacg
    20341 atcgccccga gcgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta
    20401 ggcgagatcg ccagtccgga gtggttattc agcacttccc tggtaaagta gttggctgag
    20461 ttcagcacca tactcgggcg acccggccac agccaggaag ggataaagac atagaaatcg
    20521 cggacaattg gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc
    20581 agattctccc acggcgagaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca
    20641 tcgccgctca cattcattcc atagcgtttt agcgccagcc agaacatgcc cacaatcccc
    20701 agcacgcccg ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac
    20761 aggaagatag cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc
    20821 agcaagccaa aggcgaccgt gctgacgagg aaaaacagcc acgctttgct atcctggcgc
    20881 agaaagtaga ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg
    20941 ccggagactt cactggagaa gatctgactg taggagttaa gccggaacag caaaaagccg
    21001 ttgtgcatga agaagatgcc gacgcttacc agcgcgatgc ccatcaggat cacccacgta
    21061 agattggtct ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt
    21121 ttgcgtaggc gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc
    21181 aacgcctgca acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg
    21241 ctggtcagcg ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg
    21301 aagttaaagc gcacacggcg aaactcaaac caagtcagcg tggcaataaa cagcgtgcag
    21361 agcagccaga ccacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg
    21421 caatcgccaa cacccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt
    21481 ttttatccac cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt
    21541 cggtagtaaa cagcaccggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc
    21601 ggttaagcac acaaggaatg cccgcctgaa tcagtaagca aagcgtacca atgccctgct
    21661 ggcgggcaaa aataaagtaa ccaagatcgc actgacgaag tagcgccaga taggcgtcaa
    21721 attccagttt ttcgctcaaa atttgcagat tttcttcgct gaataactcc agccccgcct
    21781 gacgaacttc ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt
    21841 ttaccgtatc gccaaattgc tgatgaacgg cgcgcaaggc agcaatatgg tcattgctgc
    21901 ggtcaccgga gttccccacc agaatggtca ttttcccttc acgttgccga tcgttcgcca
    21961 tcgtattgag agaagggtcc atccgcgtcg ggaagtacag cagttcgccc cgcacctttg
    22021 gatgcgtttt ggcaaaaaag ctcaaatcac cgcgggtggc aaatacacag ccgactcgct
    22081 tttgcgccag gcgacgtagc gggtaaaaaa gcttatatct caagccactg gaaagctcat
    22141 acaggtctgc cccccagata tgccagaaaa actggctggg cttaatgcca ccactcagca
    22201 gagccagcca cagtgtggga ttgaactgac cgtggaaaaa aaatcgctgc tgacggttag
    22261 cttttgcttt cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag
    22321 aaagcgccgg acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg
    22381 cgtgctcgct cgtcgcggcc agcgcgtcat tgaaaaaccg caaaacggtt cggttatggt
    22441 gagggatatc cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa
    22501 aaacacgcca caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc
    22561 gcccagtgca ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg
    22621 gctgacttcc gccagaatat aaaaccgcag tgacgctttg gcgatcacca gataaccaaa
    22681 gacataagcg cccactttta gcacatcacc cactaactgc caggcaaaga gatcgcgcat
    22741 agcggtaaat ttattcgaca acagcaacca gatagcaaaa tcacgcagca accagacggt
    22801 aaaactcgcc gccgccactg ccggtaagac gaattttagc gatttaacca cttcccgggt
    22861 gatatcgcgc ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc
    22921 cgtaatgaat tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc
    22981 atcccagcta tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt
    23041 caccgacgta atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc
    23101 ccagctgggt ttcagatagc ttaacgggat gacaccacgt ttgatcaaca tgatggcggc
    23161 aggaattacc accagcgcgg gaatcagcgc cagacccagc aacgcccctt cataaccgcc
    23221 caaacggtaa ctgacgtagt acgcgagaac gccaatcaag ctgccgacaa tcagcgataa
    23281 cgcattacct gcggcatcgc gaaagccttt catcagcgcc agtaacaggt tgccccaggc
    23341 gatccccatt tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa
    23401 caatccctgg ctgattggcg cagctgccag cacaaaaacc agcgccatca gcgtagagaa
    23461 accaagtacc atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg
    23521 gtactgggca acgtatttgg ttacaccgtt aaagatgcca gccccggcaa gcacgccgag
    23581 cacggtaatc aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac
    23641 cgccagcaac ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt
    23701 ccacaaggac gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta
    23761 ccgtacgctg attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc
    23821 tctctttggt ggtgtagcga tcttcaccgt ggaactcacc aaagtgttcc cccgcagggc
    23881 aaccgtgcag cggaatgtaa tgaaacaccg ccatgatttc cgcttctttc agaaagttaa
    23941 tcaacgcgct ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca
    24001 cgcagccatc gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg
    24061 catcgtagta attttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca
    24121 gttgcgccca caggtatgca gcttgcagat cggacatcaa atagctggag ccaatatcgc
    24181 gccaggtata tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac
    24241 ggatgatctc ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac
    24301 cacccgccgt gtagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc
    24361 ccagtgcacg ccctttgtaa gtggacatca cgccctgagc ggcatcttcc accacaaaca
    24421 aattatgctt tttcgccaac gccataatgg tgtccatttc gcaggccaca cccgcgtaat
    24481 ggaccggcac gataacgcgc gttttgtcgg tgatcgctgc ttcaatcaac gtttcgtcga
    24541 tgttcatggt gtccgggcga acatccacaa agacgatttt cgcgccacgc agcacaaagg
    24601 cattggcggt ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat
    24661 cgagcagcag cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg
    24721 cgctgccaaa acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac
    24781 acagtttgcc gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg
    24841 gtgcgttaaa tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc
    24901 gcaccacttt gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc
    24961 aaagttgttt taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct
    25021 gcaccgcgtc cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag
    25081 acatagccgc gaatatcgcc ggacgccgca cgtagaatca gacattgatg atcaaaggtg
    25141 ccgcgcacgg cattttcaat ccactgtgca taaaagcgac cgctggcgtc aggcgcatac
    25201 cacggcgcac gaaaacggct ttgcgcaaat gcggcgctag ctaactgacg taatgcggga
    25261 atatcggtct cttgtgccac gacagcaccg ctatcactga cattgttcac gggtagcgcc
    25321 aaatcaactt caccttctac cagggagaat cccagctgtt gcagggcatc cagttcaccc
    25381 gtatttgatg ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacgc gtctggcgtc
    25441 aggagcggtg cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaaggcgttt
    25501 tcccaggtta gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg
    25561 gccgtagcca gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca
    25621 gccgttacgc caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac
    25681 tgtctgcaca aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc
    25741 aaatccgcgc ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat
    25801 ggaggtaatc tccagttcac cacgctccga cggcttcacc tgctttgcgt actccacgac
    25861 tttactgtcg tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt
    25921 ttcttccagc gagatagcgc ggaaattgtc gtcaaactcc accacgccaa agcgttcctg
    25981 gtccatcacc tgatagccaa ataccgtcgc cccttccgtg cgcgccgcaa catgacgcag
    26041 cttcggactg aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc
    26101 accgttgagg aaggtttcac cgatgataaa ggcctgcacc agaccgtccg ggctgggctg
    26161 ttcggcgtat tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata
    26221 acctttatct tccggcgtag tgatgatgag aatttcgcgg ataccggcca acatcagcac
    26281 cgacagcgga tagtaaatca ttggcttatc gtaaatcggc aacagttgct tcgatacgcc
    26341 gcgcgtaatc ggatgcaatc gggtgccgga accgcccgcc aggataatac ctttcatgtt
    26401 tgcctccgct gaaaattagc ctttcagacc taaacgctcg ccctgatagc tgccgtcctg
    26461 cacctgcttc caccagcttt cattagccag ataccactgc accgttttac gcattccact
    26521 ttcaaaggtt tcctgtggca gccagccaag ttcacgggca attttcgaag catcaatggc
    26581 ataacgcaga tcatgccccg gacggtcagc gacaaaggtg atcaagtcac gatagtgcac
    26641 cacgccgtgc ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg tttccacaac
    26701 atcgagattc ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt
    26761 ggtcgccacg caatacagcg cgcgggcgtg atcttccaca tacagccagt cacggatttg
    26821 ctgcccgttg ccatataccg gcagcggttt acccgccagc gcgttgagga tcatcagcgg
    26881 gatcagtttt tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgataagcgt
    26941 cggcagaccg taggtccgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc
    27001 ggaataaggg ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg
    27061 caggtcacca tatacttcgt cggtggagat atgatgaaaa cggaacgctg attttttatc
    27121 ttccgtcagc gtattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat
    27181 gttggtttca ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc
    27241 cagatgcatg acacagtctg gctgatgctc agtgaatacg cgtgccagtt ctgcccgatc
    27301 gcagatatca actttctcaa aggcaaagcg atcgctttgc gcgaccggtg ccagcgacat
    27361 caggtttccg gcgtaggtca gcttatcgac cactaccacc gcgtcgctcg tttcgttgat
    27421 gatataacgc accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct
    27481 catcagcgcc agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg
    27541 ataactttaa actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc
    27601 tcgtcaagct gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta
    27661 ggctcaacaa ccagagtttc gccgctatgc cactgggcga tcagttcagc gatttccatc
    27721 gccgggcttt cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt
    27781 ttcagttcac tggcgcgttt atcggtagcc gccaggcaat cagccaccgc cgctttcacc
    27841 tgatcgataa cccagaacgg tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc
    27901 gcctgctggg ggttctgtgc cacgataaac cacggatcaa cagcaatgca gtgaccgccc
    27961 acgccagggc caggctgaag aatattaacg cgagggtgac gattcgccag gcgaatcagt
    28021 tcccagacgt taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata
    28081 ttcacatcgc ggaagctgtt ttcggtgagc ttacacattt ccgccgtccg cgagttagtg
    28141 acgacacact caccttcgag gaaaattttg tacagttcgc tggcgcgggt cgaacaaacc
    28201 ggcgtcatac caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt
    28261 aacacgcgtt ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg
    28321 aaagtgagat ccggacgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc
    28381 ggcgaggtgg attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga
    28441 gcagccgatt caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtgggtaca
    28501 gcaatcagcc aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct
    28561 acggcagttt ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg
    28621 atggtatcaa ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca
    28681 aacgctgctg ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa
    28741 ctcatagtga tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat
    28801 caccatacgg gttatgggcg cggctcatag tttgatattc gttttcgtct tttaaaagac
    28861 gcgtcacttc ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg
    28921 tcaccgcttc cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag
    28981 gcgcttcttc ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcaacc
    29041 agacaaacgg taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa
    29101 tgcgattgac cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt
    29161 cctggtgcgt ggtggcaatg tctgccaacg cgtggcagat ttcttcaaaa ccacgaccga
    29221 aactctcacg cctgtgaccg gtcaccagaa tcatcttttt attggggtcg ataaacgggt
    29281 aatttgccac cagttctgaa cgcagcgtgt cgctgctcat cacctgatca cgcacccata
    29341 acagtgcatc aatgactgta ttaccagtaa tgaagattcg gctatccgca acgttttcac
    29401 gcagcaagtt ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg
    29461 tcaatgtacg gttagcctct tccggccacg gcgaatagag atcgcccgtg cgtaaaccag
    29521 cctcaacgtg accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg
    29581 ttgttgtgtc gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta
    29641 gcccttccag aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga
    29701 gatcgtagtc aggtacaatg gaaaagagtt tcagcacctg atcgagcatc tcccgatgct
    29761 gcgcagtgac gcaaacttta gcctcaaaaa aaggagcttt tgccaacgca tgcaccaacg
    29821 gagccatctt gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc
    29881 tcttcgaata agcggcgagc gcctttgcgc tcgccgcagc agtgttgcta tttcgagcaa
    29941 cggcgggtta atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc
    30001 aggaaggcac gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga
    30061 taggtctgaa aacgcggatc cagggttgga ccaacattca gggtgtttaa catggcccga
    30121 ttctgatcat agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc
    30181 tggagcattg gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca
    30241 tctgtcgcac tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ctcaatgctg
    30301 ttcatccggc ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgagctttc
    30361 atctggatgg tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca
    30421 cgctggctgg caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttcg
    30481 gcaataagct tcacgctgtc attgaccgcg cgggtaaagt ctccggggat aaactggatg
    30541 ttgttaatca tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgt
    30601 ttgtaatagt cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata
    30661 acaaactctt tataggcttc gtccatgacc gatggttgat cggcagaagc catgtttgaa
    30721 cggacatcca ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc
    30781 gttggacgat cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc
    30841 agcgcgatta acgcaaacgc cagccccatg ccaataatcc atagcttccc agcccacaag
    30901 gtacgaaaca acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc
    30961 attggttgtg tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc
    31021 tacgcagtct gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc
    31081 aatatccata gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa
    31141 aatgagaata ttctgccagc acgccaatgg aagcgagcag tgctgcggca agggtaatca
    31201 gcacaaacgc ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac
    31261 ggtcaggaga gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat
    31321 ccattagcgg aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttgc
    31381 cctgggtcgt ttcgagcagg atccagataa cggtaaaacc aatcagcgta ctgcccgcat
    31441 cacccataaa gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca
    31501 ggatggcggc gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca
    31561 aaatcatacc gattgctgca aacgagacgc aggacaaccc gcccagcaag ccatcaatgc
    31621 catcaaccat gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac
    31681 caaacggtcc gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa
    31741 gtttgccgaa caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta
    31801 cgctgatatc aaaacggtca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga
    31861 gatagagaga tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg
    31921 cgtaaaccga aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg
    31981 gtttatccac taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg
    32041 tgaataaaaa aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc
    32101 tcagagaaaa ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc
    32161 gttccgaacc caccagtcct gtgcaaaaat taaccatttg atatgcaaat tgcataacat
    32221 gccagcaaat tccaggaatt ttcctaacat cgccagcgcg gcattaagat tacagatata
    32281 aaagcaaaac gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt
    32341 catcatttcg aagaaatcgt cattggtctt ggtcatggcc agtttattaa tgaggaattc
    32401 cattgcatcg atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag
    32461 ttcttcctga gtcgtgagca gctcttcttt acgggtaccg gaacggttgt agtcgatagc
    32521 cgggaagacg cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc
    32581 tttaaactct tcgtaaataa cttcgtccat tttagaaccg gtatcgataa gcgccgtcgc
    32641 gataatggtc aggctgccgc cctcttccac gttacgcgcc gcaccgaaga agcgtttcgg
    32701 acgatgaagg gcattggcgt ccacaccacc ggtcaacact ttacctgacg ccggaacaac
    32761 agtgttgtaa gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt
    32821 gtgctcaacc aggcgtttgg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga
    32881 tgcgggttcg tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt
    32941 tacttcttcc ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta
    33001 agcaatgctc tgagcaatgt tctgcagcag catagtttta ccggctttcg gcggtgccac
    33061 aatcagacca cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcggttaa
    33121 atcttccgta gaaccgttac cacgttccat acgcagacga gagtttgcgt gcagcggggt
    33181 taagttctca aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt
    33241 aactttcagc agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat
    33301 ggtatcacca gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc
    33361 aggaccggcg aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat
    33421 ctccagtacg ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc
    33481 aaaaataatg tcctgcttac gcatacgagc caggttttcc agccccatat tttcgccgag
    33541 agtgatcagc tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt
    33601 tcttaaactt ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat
    33661 gcctgtttac ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga
    33721 actgaaacga caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga
    33781 taaaacggga agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa
    33841 gtcaacacta agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg
    33901 aacatgaaat tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac
    33961 agtgcaccca ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata
    34021 ccacggatgc catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg
    34081 gtcagtttgc cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac
    34141 ggaccgcacc actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc
    34201 gtgtcaaaac tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga
    34261 ataagcctgg cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt
    34321 atttcaccgg atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc
    34381 aaaacacatt taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa
    34441 atccttgaaa aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg
    34501 acgctggcgg gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg
    34561 tttcttacgt caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg
    34621 aatcaaccac gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc
    34681 gacgcagaac cgctggcgga agctaccggc ctgaagctgg gtctggctta cggtggtgat
    34741 ggctacgaca aacagctgaa agtgctggaa agcggcgttg acattctgat tggcactacg
    34801 gggcgtttaa ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtggtg
    34861 gtactggacg aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg
    34921 ttccgccgta tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg
    34981 taccgggtac gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa
    35041 ccggaacaga aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa
    35101 aaaatgcgtt tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc
    35161 gccaatacca aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt
    35221 gtcggtttgt tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt
    35281 acccgtggcg atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt
    35341 ccggcagtga cgcacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac
    35401 cgtattggtc gtacaggtcg cgcaggcgca agcggtcact ctatcagcct ggcgtgtgaa
    35461 gagtatgcat tgaatttgcc tgctattgag acctatattg gtcactcaat tccggtaagc
    35521 aaatacaatc cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgccca
    35581 cgtacaggca atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa
    35641 ataaaatgtc atactcaaaa tcattcaaac tgtatcaaga tggcaagaga atgaatcccg
    35701 atgagcatac tgtagtaagt gattcaggtg aaagaatgca gccaacacag agacagattg
    35761 aaggatgaag agtatgggtt ccacctcgtc gctgtatgca gccattgatc tcggttcgaa
    35821 tagttttcat atgctggttg tgcgcgaggt ggctggaagc atccagacgc tgacgcgaat
    35881 aaaacgcaaa gtgcgtctgg ctgctggcct gaatagcgaa aatgccctgt ctaatgaagc
    35941 aatggagcgc ggttggcaat gtctgcgcct gtttgctgaa cgtctgcaag atatccctcc
    36001 ctcgcaaatt cgcgttgtcg ctacggcgac attacgtctt gccgtcaatg cgggtgattt
    36061 tattgccaaa gcgcaggaaa tcctcggttg tccggtacag gtgatcagcg gtgaagagga
    36121 agcacgtctg atttatcagg gcgttgctca cacaactggc ggtgccgatc agcgcctggt
    36181 ggtggatata ggcggtgcca gtactgaact ggtaaccggc acgggtgcac aaaccacctc
    36241 gttgttcagc ctgtcgatgg gctgcgtcac ctggctggaa cgctattttg ccgatcgtaa
    36301 tctggggcag gaaaattttg atgctgcaga aaaagcggca cgcgaagtgt tacgtccggt
    36361 tgccgatgaa ttacggtatc acggctggaa agtgtgcgtt ggtgcttccg gcaccgtgca
    36421 ggcgttacag gaaatcatga tggcacaggg gatggatgaa cgcattaccc tggaaaagtt
    36481 gcagcaattg aaacagcgag ccattcattg cggtcggctg gaggaactgg agattgacgg
    36541 gctgacgctg gaacgtgcgt tagtgttccc gagtggtctg gcgatcctga tcgccatttt
    36601 taccgaactg aatattcagt gtatgaccct ggcgggcggt gcgctgcgtg aaggcctggt
    36661 ctacggcatg ttgcatctgg ccgtcgagca ggatattcgc agccgtacgc tgcgtaatat
    36721 tcagcgccgc tttatgatcg atattgatca ggcgcagcgc gtagccaaag ttgcggctaa
    36781 cttcttcgat caggtggaaa aggaatggca tcttgaagca ataagccgcg atttgctcat
    36841 cagcgcctgt cagcttcatg aaatcggcct gagcgttgac tttaaacaag cgccgcaaca
    36901 cgctgcttat ctggtgcgta atctggatct tcccggtttt acccccgcac agaaaaaatt
    36961 gctggcgacg ctactgctca accagactaa tccggtcgat ctctcatcgc tgcatcagca
    37021 aaatgccgta ccgccgcgcg tcgcagaaca actctgccgt ttactccgcc tggccatcat
    37081 ttttgccagc cgtcgccgtg acgatctcgt gccagagatg acattacagg ctaaccatga
    37141 actgttgacc ttgacgcttc cgcaaggttg gctaacccaa catccgctgg gtaaagagat
    37201 tattgctcag gaaagccagt ggcagagcta tgtccactgg ccgctggaag tgcattaatg
    37261 agtaagtgcc ggatgcgatg ctgacgcatc ttttccggcc ttgattattt ccctcgtttt
    37321 gccgccatca tcgctttcag attcgccaga tggctttgcc ctttctgcat ccgttcttcg
    37381 gcgctgacca ctttgcgctc ctgttcccaa atcagatcat cctgcggcag ctccagcaaa
    37441 aagcggctcg gctccgggcg caccagctcg ccgtactgac ggcgttcttt acacagcgta
    37501 aaggtcaatt ccttctgggc gcgggtaatg ccgacatagg ccagccgccg ctcctcatcg
    37561 atattatctt catcgatgct gctctggtgc ggcaaaaacc cttcttccat accgaccatg
    37621 tagacataag gaaactccag cccttttgac gcgtgcagag tcatcagttg cacctgatcc
    37681 agctcttctt cactctcacc acgctccatc atgtcgcgca aagtaaagcg cgtcaccacc
    37741 tgggtgagcg tcatcggctc atccagttcg ctgccttcca gcatctccgt catccagcta
    37801 aacagttggt tgacgttctt catgcgcatt tcggcggctt tcgggctggg cgatgtttcg
    37861 tacagccagg attcataatc catgccgtgg atcagatcgc gcaccgcggc aatcggctcc
    37921 cgctccgcca gacgctggat ttctgccaac cagtgagtaa agcgggtcaa tgcttcataa
    37981 ccacgtccgc taagcgtctg actcaggccc atatcaaagc tggcggtaaa catgctttta
    38041 ttgcgcgtca tcgcccactc acccagcttt ttcagcgtcg ccgggccaat ctctctcttc
    38101 ggcgtgttaa cgatacgcag aaatgcgctg tcatcgtccg ggttagtcag cacgcgcaga
    38161 taagccagca agtccttgat ttcaggacga gagaaaaacg acgtaccacc agaaattttg
    38221 tacgggatgc ggttttgcat caggaatttt tcaaacactc gcgactgatg gttaccgcga
    38281 taaagaatag cgtaatattt gtactgcgtt ttattgacga agtgatgcgc aatcagttca
    38341 ccagtgacgc gctcggcttc atgttcttcg ttattcgcgc ttaaaacttt aagctctgtg
    38401 ccgtaaccca gttcggagaa cagacgcttc tcaaagacgt gcgggttatt ggcgatcagg
    38461 atgttcgccg ctttcaaaat acgtccggaa gagcggtagt tctgctcaag cttaatcacc
    38521 ttcagcgccg gaaaatcctg actcagcagc accaggtttt gtggacgtgc gccgcgccag
    38581 gagtagatcg actggtcgtc atcgcccacc acggtaaagc gcgcgcggct gcccaccagc
    38641 agtttcacca gttcatactg gctggtgttg gtgtcctgat actcatccac cagcagatag
    38701 cgaattttgt tctgccagcg ctcacggact tcttcattgc gttgcagcag caacgtcggc
    38761 aataaaatca gatcatcgaa gtcgagaaca ttgcacgctt tcaggtgtgc atcatacagc
    38821 ccataacaat gggcaaaaat acggtcccgc tcgccaatcg cactggctgc cgcctgggac
    38881 ggtgttttga gatcgttctt ccagttagag atggtcgaaa tcaactgttg caggagaact
    38941 ttgtcatctt caatcagccc ttcggtcaac tctttgagca acgccagctg atcggtatcg
    39001 tcaaacagcg aaaagttcgc cttcatcccc agcgccgcat actcacgctt gatgatatcc
    39061 agccccagcg tgtggaaggt tgagatcatc agcccacgcg cctctttgcg acccagcgtc
    39121 tgtccgacac gctctttcat ctcgcgcgcc gccttattag taaaggtcac cgccgcgata
    39181 tgtcgcgcct gataaccgca accgcggatc agatgggcga ttttattggt gatcacacga
    39241 gttttaccgg aacccgcgcc cgccagcacc aggcaggggc cggtaacgaa ttcgacagct
    39301 tgttgttggc cggggtttag acgcataggt gtattgctca atcttcgaac ggggggagga
    39361 ttgtagcaga atcgcatttg aatgtgaccg cgtgtaagcc gctaaccggt tggctgcgga
    39421 tgtttaaatc agagagggaa gaccgaaacc ggagttcatc gtgaatccat tagacttaga
    39481 aaatatcggg
//
LOCUS       DAJTYD010000017        37216 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTYD010000017 DAJTYD010000000
VERSION     DAJTYD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.017"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..799)
                     /locus_tag="OO585_000801"
     CDS             complement(<1..799)
                     /locus_tag="OO585_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR6628478.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEV"
     gene            complement(813..1244)
                     /locus_tag="OO585_000802"
     CDS             complement(813..1244)
                     /locus_tag="OO585_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628479.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1244..1447)
                     /locus_tag="OO585_000803"
     CDS             complement(1244..1447)
                     /locus_tag="OO585_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6628480.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1560..2408)
                     /locus_tag="OO585_000804"
     CDS             complement(1560..2408)
                     /locus_tag="OO585_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628481.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILNIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2504..3763)
                     /locus_tag="OO585_000805"
     CDS             complement(2504..3763)
                     /locus_tag="OO585_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6628482.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3954..4817)
                     /locus_tag="OO585_000806"
     CDS             complement(3954..4817)
                     /locus_tag="OO585_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6628483.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4944..5660
                     /gene="rph"
                     /locus_tag="OO585_000807"
     CDS             4944..5660
                     /gene="rph"
                     /locus_tag="OO585_000807"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6628484.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5726..6367
                     /gene="pyrE"
                     /locus_tag="OO585_000808"
     CDS             5726..6367
                     /gene="pyrE"
                     /locus_tag="OO585_000808"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6628485.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6404..7000)
                     /gene="slmA"
                     /locus_tag="OO585_000809"
     CDS             complement(6404..7000)
                     /gene="slmA"
                     /locus_tag="OO585_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6628486.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7107..7565)
                     /gene="dut"
                     /locus_tag="OO585_000810"
     CDS             complement(7107..7565)
                     /gene="dut"
                     /locus_tag="OO585_000810"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6628487.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7543..8763)
                     /gene="coaBC"
                     /locus_tag="OO585_000811"
     CDS             complement(7543..8763)
                     /gene="coaBC"
                     /locus_tag="OO585_000811"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6628488.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8935..9603
                     /gene="radC"
                     /locus_tag="OO585_000812"
     CDS             8935..9603
                     /gene="radC"
                     /locus_tag="OO585_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6628489.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9820..10056
                     /gene="rpmB"
                     /locus_tag="OO585_000813"
     CDS             9820..10056
                     /gene="rpmB"
                     /locus_tag="OO585_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6628490.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10077..10244
                     /gene="rpmG"
                     /locus_tag="OO585_000814"
     CDS             10077..10244
                     /gene="rpmG"
                     /locus_tag="OO585_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6628491.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10342..11151
                     /gene="mutM"
                     /locus_tag="OO585_000815"
     CDS             10342..11151
                     /gene="mutM"
                     /locus_tag="OO585_000815"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6628492.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11190..11669)
                     /gene="coaD"
                     /locus_tag="OO585_000816"
     CDS             complement(11190..11669)
                     /gene="coaD"
                     /locus_tag="OO585_000816"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6628493.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11677..12954)
                     /gene="waaA"
                     /locus_tag="OO585_000817"
     CDS             complement(11677..12954)
                     /gene="waaA"
                     /locus_tag="OO585_000817"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6628494.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13367..14425
                     /gene="rfaQ"
                     /locus_tag="OO585_000818"
     CDS             13367..14425
                     /gene="rfaQ"
                     /locus_tag="OO585_000818"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6628495.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14422..15546
                     /locus_tag="OO585_000819"
     CDS             14422..15546
                     /locus_tag="OO585_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6628496.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15539..16336
                     /gene="rfaP"
                     /locus_tag="OO585_000820"
     CDS             15539..16336
                     /gene="rfaP"
                     /locus_tag="OO585_000820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6628497.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16352..17368
                     /gene="waaO"
                     /locus_tag="OO585_000821"
                     /gene_synonym="rfaI"
     CDS             16352..17368
                     /gene="waaO"
                     /locus_tag="OO585_000821"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6628498.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17385..18380
                     /locus_tag="OO585_000822"
     CDS             17385..18380
                     /locus_tag="OO585_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6628499.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18390..19082
                     /gene="rfaY"
                     /locus_tag="OO585_000823"
     CDS             18390..19082
                     /gene="rfaY"
                     /locus_tag="OO585_000823"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6628500.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            19108..20133
                     /locus_tag="OO585_000824"
     CDS             19108..20133
                     /locus_tag="OO585_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR6628501.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20218..21201
                     /locus_tag="OO585_000825"
     CDS             20218..21201
                     /locus_tag="OO585_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6628502.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21247..22500
                     /locus_tag="OO585_000826"
     CDS             21247..22500
                     /locus_tag="OO585_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6628503.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22570..23541)
                     /gene="rfaC"
                     /locus_tag="OO585_000827"
     CDS             complement(22570..23541)
                     /gene="rfaC"
                     /locus_tag="OO585_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6628504.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(23545..24591)
                     /gene="rfaF"
                     /locus_tag="OO585_000828"
     CDS             complement(23545..24591)
                     /gene="rfaF"
                     /locus_tag="OO585_000828"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6628505.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24601..25533)
                     /gene="rfaD"
                     /locus_tag="OO585_000829"
     CDS             complement(24601..25533)
                     /gene="rfaD"
                     /locus_tag="OO585_000829"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6628506.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25747..26943
                     /gene="kbl"
                     /locus_tag="OO585_000830"
     CDS             25747..26943
                     /gene="kbl"
                     /locus_tag="OO585_000830"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6628507.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26953..27978
                     /gene="tdh"
                     /locus_tag="OO585_000831"
     CDS             26953..27978
                     /gene="tdh"
                     /locus_tag="OO585_000831"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6628508.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28217..29251
                     /gene="waaH"
                     /locus_tag="OO585_000832"
                     /pseudo
     CDS             28217..29251
                     /gene="waaH"
                     /locus_tag="OO585_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29238..30197)
                     /locus_tag="OO585_000833"
     CDS             complement(29238..30197)
                     /locus_tag="OO585_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6628509.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30201..31484)
                     /gene="envC"
                     /locus_tag="OO585_000834"
     CDS             complement(30201..31484)
                     /gene="envC"
                     /locus_tag="OO585_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6628510.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31494..33038)
                     /gene="gpmM"
                     /locus_tag="OO585_000835"
                     /gene_synonym="pgmI"
     CDS             complement(31494..33038)
                     /gene="gpmM"
                     /locus_tag="OO585_000835"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6628511.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33283..33714
                     /locus_tag="OO585_000836"
     CDS             33283..33714
                     /locus_tag="OO585_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6628512.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33856..34107
                     /gene="grxC"
                     /locus_tag="OO585_000837"
     CDS             33856..34107
                     /gene="grxC"
                     /locus_tag="OO585_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6628513.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            34170..34637
                     /gene="secB"
                     /locus_tag="OO585_000838"
     CDS             34170..34637
                     /gene="secB"
                     /locus_tag="OO585_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6628514.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34637..35656
                     /gene="gpsA"
                     /locus_tag="OO585_000839"
     CDS             34637..35656
                     /gene="gpsA"
                     /locus_tag="OO585_000839"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6628515.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35736..36557
                     /gene="cysE"
                     /locus_tag="OO585_000840"
     CDS             35736..36557
                     /gene="cysE"
                     /locus_tag="OO585_000840"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6628516.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36610..37083)
                     /gene="trmL"
                     /locus_tag="OO585_000841"
     CDS             complement(36610..37083)
                     /gene="trmL"
                     /locus_tag="OO585_000841"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6628517.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9222 a         8859 c         9269 g         9866 t
ORIGIN      
        1 ttacctccag acgtttagcg agccagcgtt gcgacaggcg ggtgagttct cctttgcgct
       61 gctcatattc catgcccata tcaattaacg tgatattggt gctttcgaga taggcgaggt
      121 gttcaagctg tgctgcgttc atgctgtccc gtggttcgcc agtaatgcca tttgcctgcg
      181 cccactgctt cgccgtcatg ccacccagca cgatacgggc gatcatgttg ctctcgttgc
      241 tgtagtggcg ctgctgcgtc gttttgccca tctcggcacg ggccatattc agcgcatcgc
      301 acattggctt aaagttgttt gcggcactga gacgggcttt tagctggcga cgatagcggg
      361 cggcgatttc aggcacgcta cgctgtaatt cttcctcgca ctggatgaaa taacggcgga
      421 tagcgcggcc ctgctcgttg cgttcaacca ttgccacttc tttagccata tccagcgata
      481 gcaggtaatc atgctctatt tgctggcgag tttttgcgct cgcccgtttt ggcgtgctca
      541 aattttcaac acggataaaa tcggttccgg caataaaacc gtactggtca attcggcctt
      601 taatccagtt agtaaagtcg cggcccaccc ccagcgcttt atgcaacgcc ttagcgctca
      661 caatattggc ttcacgcccg ccaatgcgtc cggcaataac gggaacaata gcggcaaagt
      721 cgttgccgtt aattactccc gtgttaacgt tgggttgcgg ggcggccccc gaactgaatc
      781 ggcttttttc gatagtcata ttttcggctc cgttatgcgg cggtgagatt gtccgggtat
      841 agattgagaa tgtcggcaat ttcaggctga gaaagtccct gatagccgcc agcgtcggcg
      901 ttatggttca caagccgaat aacttttaga acgtcaccgc gcccggtaaa gcgatagcgt
      961 aaatgtgacc ctatcccgtc ggggttcttt tcgtctatac gctcaagctg aatatcaagc
     1021 ctgcgctcta actcgctggc gtagttcctt ccagacgaaa ggcggcaata acgcagaatg
     1081 tcattttctg tccatccctc tataccagta cgcagcatat agactctggc acgatgcttt
     1141 ttcggtgtgc gctttggtgc ttgaagggat tttgtaaggg gcgtaacatc agatacgcga
     1201 atatctgaat tagccgcctg taatgggcgg tttttctttt ccatcaggcc accttcccgc
     1261 ggcgttctgc cagccagttg tgaatctcaa ccgcatcaaa cgcagttatt ttttctccca
     1321 gctttaccgg acgtggtagt gaaccgtttc ttactttacg gtcgatggtg gatacactaa
     1381 cgcccagcaa ttcagccagg cggaagcgtc tgatatagcc ttttgtaggg atagtgtcga
     1441 tgttcatgat tcccgtaacc tcttagcgat agttgttgct tcgttacggg tatttaatgg
     1501 cgtgcacgct caaaaaggaa tgatcgtatt ttttactatc gtgatagtaa aatttactat
     1561 cacctttttt tccctgccgt ggtgcgttct tccggtgttc tgtctgcgtc ggctatgagt
     1621 ttcttcaggt aatctatgga tggctcatca tagccgcccc ccaaaagcgc ccagtggttt
     1681 attgtggcgt tcgcccatgc ttcgttggtt tccttcccgt tcttcccttt acactcttct
     1741 gggtagtgcc gcttcacata aatcaacgcc aagaggcaaa tttcgcgctt ttgcctgtgg
     1801 cgctctctgt ttccgtgctc aacgttttta acatcctcat aaagagcttg taagtttatg
     1861 cgagtttcgg ggctgtatat gggctggttt ttgtccatgc attcgccgat atattcaacc
     1921 cacggccttg ttatgtacaa atcctctacg gatatttcgt ggaaggcatg ttttgtataa
     1981 tcgagaaaaa aataataatt cgcacttacg tctagttctc tttttctgtg tgcggggctg
     2041 aatatgcgtg cgggagtgtc aatagaatca agccctgcga tgttgagtat ataggaggcg
     2101 ttaacatgcc atagcccaag cacatggcaa tctatatgat ttacaacgcc tgccttaaag
     2161 cttttttcgt cctctacttc agtgattatg tcggcaagta ataagcagtc ttctttgacg
     2221 tctgggaata aagagcaatt gcggtaaaga ctcgcgatgc tatgtaatgt gccgtcgtag
     2281 ccgcaaaggt acgcgctcaa atatatagat tctgccgccg ccataatcaa aagatcatca
     2341 atatctattt ctaataattc ggctgctctg ttaagcctga aaaaaggggc gggtggtaat
     2401 tttttcattg tgtcaccttt tacgctgcgc cttattttgt gattgagcca ggcggtaagg
     2461 cttcccgctt ttcgccccgt cgagctaggc tcaatctatt cagttagttc gcccttctga
     2521 gaggcgttac gttgtagtct tttccgcttt ccagtgccac cagcagatca gcccactgtg
     2581 ccagtgctgc tttacgctca tcaaaatact gatggcggtt ataaatcccc tctacgccct
     2641 taatgcgatg attgaggcat cgttccgcca caacaggatc aacacccaac gctgctaaat
     2701 gagtacgtgc ggtgcgtcga aagtcgtgaa tcgtgaaatt aggcacatcc ggcatttcgg
     2761 cgcgaacctt agccagtgct acgggtaaag tgctttcctg aatatgtggg atcattctgt
     2821 tttgcatctt cctggccgga agcacccatg cgctattaca tgaaaaggtg tgtagctctc
     2881 tcaaccattc aacggctggg gaaggtagag gtatatcaat agggtctccg tttttgctgc
     2941 gttcttccgg caaatgccat accgcaccat ctaaatcaaa ctcttcccat cgtgcggcgc
     3001 ataattccat tttgcggacg cataacgcta acagtaattt gaacgtgatt tcgttctggc
     3061 gactgaatcc cttagccgtg cgcatggctt tgaatagctg gattaactca tcgcgggtta
     3121 accagcggtc acgggcagct tctttccctc cggcgtctgc cacctcaaag gctgaacagg
     3181 ggttaatctc tagcgcgtgc cgtttgattc cgtagtcgaa tatgcggcgc gtccatctca
     3241 gcacgtccgt tgctatggtc ggcgctccac ggtcaacaat acctttcagc atgtcatcga
     3301 tatggcgcgg tttcacgtcc tctaccttca tgctgccaat gcaggggttt atatctttgt
     3361 cgatacggcg gcggagtata tcggggtgct tccagcgcgg gaggatctga cgctcaaagt
     3421 attcagcggc aagctctgaa acgcgcatag cgttcttctc tgcttccatc ttcgccagtg
     3481 cttcggtttt gcgcttctgc ttctctcctg caacgtcata gcccagcgca acgcgagccg
     3541 atagctcttt ggctgtctct ctggcctttg atagtgatag ctcgctatac gaaccaatta
     3601 gcatggcgcg ggacttccct gcgagtttat agcggaatct ccatgtgggg gttttgtcgg
     3661 cttcacggta acgtaggtat aaaccgttac cgtctgcccg cccctcgaat cgctctccgc
     3721 tcttaatcca tgcgcggatc tgcatgtctg ttagttttgg cattttgcga atatccaaac
     3781 tttgaatagg gggtgtaccc aaagcaatgt acccaaacct cattgggtac acctttgtgt
     3841 acaccataat catgcgcttt gttgcgtctt tatgatagcc catgataaca aaaaagcccg
     3901 ctgggttgcg ggcttggtgt gatttatgcg tcgtcctaaa tgttgctgat agcttactcg
     3961 atattctgaa tctgctcgcg catctgctca atcaacactt tcagctcaat cgcggagttt
     4021 gtcacttcgg cattgataga cttcgacgca agagtgttcg actcgcggtt gaattcctgc
     4081 atcataaagt ccagacgacg accaacggct tcttttttct tcagaatgtt gtaggtctct
     4141 ttgacatgcg cttccaggcg gtccagttct tcggcaacgt caattcgttg tgccagcaga
     4201 accagctcct gctccagacg gttgttttcc aactgaacct gagcatcttc cagcttcgcg
     4261 accagacgct cacgctgcca ttgcaggatt tccggcatat gggcgcggac tttgaccact
     4321 tcggcggtga cgccttccag acgctgctcg atcaatgctt tcagtgcctg accttcggtt
     4381 tcgcgcgcga caataaagtc gtccagcgta ccatccagcg ccgcgagaat ttcagcggca
     4441 atggcgtcaa gatcctgctc ctgggctgcc atcacgcccg gccagcgtag aatatcaacc
     4501 gggttgattt ccccttcgtc actctgcatt tttacccagt tcgcggcagt taccagctgt
     4561 ttagccagtt tttcgttgag gatcagctca ccttgcgcgc taatatctgg ctcatagcgc
     4621 agggtacatt ccactttacc gcgcgtcagg cgagaacgaa tacgctcgcg aacgacaggt
     4681 tcaaggctac ggaactgctc cggcagacga aagtaagttt ccagataacg ctggtttacc
     4741 gagcgcattt cccaggttgc gctcccccat tcacccttga tttcacgccg ggcgtaggcg
     4801 gtcatactgc ggatcataga cgttcctgtt tataaaagga gaggtggaag gattatagcc
     4861 atcgatgcct tgtcaggata ggaataaccg ccggaagtcc gtataatgcg cagccacatt
     4921 tgtttcaagc cggagatttc aatatgcgtc cagcaggccg tagcaataat caggtgcgtc
     4981 ccgttaccct gactcgtaac tatacaaaac atgcagaagg ctcggtgctg gtcgaatttg
     5041 gcgataccaa agtgttgtgt accgcctcta ttgaagaagg cgtgccgcgc ttcctgaaag
     5101 gtcagggcca gggctggatc accgcagagt acggcatgct gccacgttct acccacaccc
     5161 gtaacgctcg tgaagcggcg aaaggtaagc agggtggacg cacaatggaa atccagcgtc
     5221 tgatcgcccg tgctcttcgc gcggcagtag atttgaaagc gctgggtgag ttcaccatta
     5281 cgctggactg cgacgtgctt caggctgatg gtggcacgcg taccgcgtcg attacgggtg
     5341 cctgcgtggc gctggcagat gcgctacaga agctggtgga aaacggcaag ctgaaaacca
     5401 acccgatgaa agggatggta gccgcagttt ctgtcggaat tgtgaacggc gaagcggttt
     5461 gcgatctgga atacgttgaa gactctgccg cagagaccga catgaacgta gtgatgaccg
     5521 aagacgggcg catcattgaa gtgcagggga cggcagaagg cgagccgttc acccatgaag
     5581 agctactcac cttgttggct ctggcccgag ggggaatcga atccattgta gcgacgcaga
     5641 aggcggcgct ggcaaactga tttttaaggc gactgatgag tcgccttttt tttgtctgta
     5701 gaaaagtaag atgaggagcg aaggcatgaa accatatcag cgccagttta ttgaatttgc
     5761 gcttggcaag caggtgttaa agtttggcga gtttacgttg aagtccgggc gcaaaagccc
     5821 ctatttcttc aacgccgggc tgtttaatac cgggcgcgat ctggcactgt taggccgttt
     5881 ttacgctgaa gcgttggtgg attccggcct tgagttcgat ctgctgtttg gccctgctta
     5941 caaagggatc ccgattgcca ccactaccgc cgtggcgctg gcggagcatc atgaccttga
     6001 cctgccgtac tgctttaacc gcaaagaggc aaaagaccac ggtgaaggcg gcaatctggt
     6061 tggtagcgcg ttacaaggac gcgtaatgct ggtagatgat gtgatcaccg ccggaacggc
     6121 gattcgcgaa tcgatggaga ttattcaggc taatggcgcg acgcttgctg gcgtgttgat
     6181 ttcgcttgat cgtcaggaac gggggcgcgg cgagatttcg gcgattcagg aagttgagcg
     6241 tgattacaac tgcaaagtga tctctatcat caccctgaaa gacctgattg cttacctgga
     6301 agagaagccg gaaatggcgg aacatctggc ggcggttaag gcctatcgcg aagagtttgg
     6361 cgtttaaaga aactcgccgg atgaaaagtc atccggcgtc atattactgc aactgtgccg
     6421 caattagcgg ccagcgggcg tcaaaatcat ccgtcgggcg gtatttaaat tcgctgcgga
     6481 caaaacgtga cagcatacct tcacagaagg ccaggatctg gcttgccagc agggtttcat
     6541 cggtggtgta accttcaccc tcacgcattc tcttttcacg caatacctga cgcagctgcg
     6601 cttcaatacg ctcgaacagc tggttgatgc gcccttgcag gcgatcctgt tcaaacatta
     6661 gcgcatgacc agtgaggatg cgggtcaggc caggattacg ctcaccaaaa ccgagaagca
     6721 gcaacacaat cagacgcagg cgcgctgtgg tgtctttctc atctttcaga atcaggttga
     6781 tgcgagtaat caggctatct tcgataaact caatcaggct atcgaacatg cgggtcttac
     6841 tggggaagtg gcgatacagt gccgcttcgg aaacgccgac agaggcggcc agttttgccg
     6901 tcgtgatacg ttggcttcca tcgctggatt ccagcatcag cgccagagac tgaagtattt
     6961 cctcgcgacg gttccttttc gcagtttgtt tttctgccat gttacaaaat acccctgaaa
     7021 ataagcactt gccaggcggc acccacgcta tgaccgcaaa cgaaatgttt gcggctatgt
     7081 tatgacgtta ttcggatgcg tatgtgttac tgacgaccag agtgaccaaa gccgccttca
     7141 ccgcggtcgg tggcgtcgaa atcttccacc agattaaatt cagcctgtac taccggaaca
     7201 aaaatcatct gggcgatgcg ttcgccaggt tgaatggtga agctgtcctg accacggttc
     7261 cacacggaaa tcatcaactg gccctgatag tcggaatcga tcaatcctac caggttacca
     7321 agcacgatac cgtgcttatg tcccaatccg gagcgcggca gcatcattgc cgccagtgaa
     7381 ggatcggcaa tatgaatcgc cagcccggtc ggaaccagcg tagtgtcacc cggagccagt
     7441 tctacggcgt cgtcgagaca ggcacgcagg tcaagtccgg cagagccaga ggtggcataa
     7501 gtcgggagcg gaaattcctt cccaacgcgc gggtccagaa tcttaacgtc gatttttttc
     7561 atcataacgg gtcacgatct cgtcgagtaa tagttggcca aggagctctt tgcgctcaag
     7621 cggtaagact ttatctccgt cctgccagaa aaggtgtaat gcgttgtttt cgctgttaaa
     7681 tccttgagtt ggctgggaaa catcgttcgc gcagatcaga tcaaggtttt tacggatacg
     7741 tttttgccgg gcgtattctt ccacattatt tgtttcggcg gcaaatccaa cgacgtaggg
     7801 tcgatggtct tttagtgcgg caacgcctgc gacgatatcg gggtttttaa ccatttttat
     7861 tgttaattca tcaccctgcg tggcctgctt tttgattttt tctggggcca cggtagctgc
     7921 gcgataatcc gccacggcgg cgcagccgat aaaaatattt tgctgctgta cagaagcatt
     7981 cacggcggct tccatttcca gcgcggtcat cacatcaaca cgtttaacaa acggtggcgt
     8041 cggtagtgaa accggacctg ataccagcgt gacgttcgcg ccacgacggg cagcggcggc
     8101 ggcgatagca aaacccatct tgccggagct gtgattagag atataacgca cgggatcgag
     8161 cggttcacgc gtcggaccgg cggtaatcat aatgttcaga tgtttcaggt cgttgacggg
     8221 cgaaaaatgc gccaccgcca tatccacaat ggttaacgga tcgagcattc gcccaggacc
     8281 gatatcacca caagcctgac tgccactgtc tggcccccag atgagcaaac cacgggaagc
     8341 aagcccctct aaattatgct gcgtggcagc ggcacggtac atctgctggt tcatggcggg
     8401 gagcacggct acaggcgcag gtgtagccag acaaatcgtc gataccaggt cattcgccat
     8461 tccggcagca acacgggcaa tcaaatctgc cgtggcaggg gcgagaatca ctaaatcagc
     8521 ccatttaccc agctcaatat ggcccatagc ggcttcggct gccgggtcca gcaaactgtc
     8581 ggaaacggga taaccagaaa ccgcctgcaa gctaagtggg gtgataaagg cttttgccgc
     8641 ttcggtcatg gctacgcgga cgtcggcccc gcgatcgcgc aaacgacgca ccagttcagg
     8701 ggttttatag gcagcaatac cgccgctaac gccgagaacg atttttttac cggccaggct
     8761 catgatgatt tttcctgttg ggtgacacca gaagttggcg attttatcac aatacttttg
     8821 ttgtcgtgcc ttcacctgag aatcactttg cgaggcgctt tccaggattg aaaactggcc
     8881 gtcgatttaa cggaacggct atgacaggat gcgagcacca caaaggaggt gaaggtgaaa
     8941 aacaattcac agctgttgat gccgcgcgaa aaaatgctga agtttggtat tagcgcctta
     9001 acggatgtcg agctgctggc gctatttctg cgtaccggaa cgcgcggtaa agatgtatta
     9061 accctggcaa aagagatgct ggagaatttc ggctctcttt atggcttgtt aacctctgaa
     9121 tatgaacaat ttagtggcgt tcatggaatt ggcgtggcga aatttgccca gttaaagggg
     9181 attgctgaac tggcgcggcg ttactacaac gtgcggatgc gtgaagaaag ccctttactc
     9241 agcccggaga tgacgcggga atttttacaa agccagctta cgggtgagga gcgggagatc
     9301 tttatggtga tctttctcga ctcacaacac cgggttataa cgcatagccg tcttttttcc
     9361 ggcacgctaa accatgttga agtccatcct cgggaaatta tccgcgaagc gataaaaata
     9421 aacgcctcgg cgctgatcct tgcacataat cacccttcgg gttgtgctga acccagtaaa
     9481 gcggataaac tcattactga acggataata aagagttgtc agttcatgga tttacgcgtg
     9541 ctcgaccata tcgtgattgg gcgtggagag tatgtttctt ttgccgaacg cggctggatt
     9601 taacccgcta tgcgcgatcc ttcgggatct ttgtctgttc gggacttgag cacatcgctg
     9661 agtcagcgta tactacgcca cctttgagaa tctcgggttt ggcatttggg cctggcaatc
     9721 gagagttcac atagaactgc gatgactggg ctgtaaagcc tgacgaggcg ccaatacccc
     9781 atacgaagct cgagctaatt tgatttttgg agaatagaca tgtcccgagt ctgccaagtt
     9841 actggcaagc gtccggtgac cggtaacaac cgttcccacg cactgaacgc gactaaacgc
     9901 cgtttcctgc cgaacctgca ctctcaccgt ttctgggttg agagcgagaa gcgttttgtc
     9961 accctgcgcg tatctgctaa aggtatgcgt gtaatcgata aaaaaggcat cgatacagtt
    10021 ctggctgaac tgcgtgcccg tagcgaaaag tactaagtac ttagaggaaa taaatcatgg
    10081 ctaaaggtat tcgtgagaaa atcaagctgg tttcttctgc tggtactggt cacttctata
    10141 ccactacgaa gaacaaacgt actaagccgg aaaaactgga actgaaaaaa ttcgatccag
    10201 ttgttcgcca gcacgtgatc tacaaagaag cgaaaatcaa ataattctcg ctttgatgta
    10261 acaaaaaacc tcgctccggc ggggtttttt gttatctgct tgcccccata ttgactgcat
    10321 ctgttcattc ctggagatgc tatgcctgaa ttacccgaag ttgaaaccag ccgccgcggc
    10381 atagaaccgc atctcgttgg tgcaaccatt cttcatgcag tggtgcgcaa cggacgcttg
    10441 cgctggccgg tttcagaaga gatctaccgt ttaagcgacc aaccagtgct tagcgtgcag
    10501 cggcgggcta aatatctgct gctggagctg cctgagggct ggattatcat tcatttaggg
    10561 atgtctggca gcctgcgcat ccttccagaa gaacttcccc ctgaaaagca tgaccatgtg
    10621 gatttggtga tgagcaacgg caaagtgctg cgctacaccg atccgcgccg ctttggtgcc
    10681 tggctgtgga ccaaagagct ggaagggcat aatgtgctgg cccatcttgg accggagccg
    10741 cttagcgacg atttcaatgg tgagtatctg catcagaagt gcgcgaagaa aaaaacggcg
    10801 attaaaccgt ggctgatgga taacaagctg gtggtagggg tagggaatat ctatgccagc
    10861 gaatcactgt ttgcggcggg gatccatccg gatcggctgg cgtcatcact gtcgctggca
    10921 gagtgtgaat tgttagctcg ggtgattaaa gcggtgttgc tgcgttcgat tgagcagggt
    10981 ggtacaacgc tgaaagattt tctgcaaagt gatggtaaac cgggctattt cgctcaggaa
    11041 ttgcaggttt acgggcgaaa aggtgagccg tgtcgggtgt gcggtacgcc gattgtggcg
    11101 actaaacatg cgcagcgggc aacgttttat tgtcggcagt gccagaagta attcatgcgc
    11161 gccggatcgc ataccatccg gcataaacgc tacgctaact tcgccatcag cgcctgatgg
    11221 acattctccg gcaggaaatg ggtgacatcg ccctgatggc gcgccacctc tttcaccaac
    11281 gatgaagaga taaacgacca ctctttcgac ggcatcagaa acacactttc cagttccggc
    11341 attaagtggc gattcatatg cgccagctgc atttcatatt caaaatccgc caccgcacgt
    11401 aggccacgaa tcagcaccgt agcgtgttga ttacgggcga agttcgccat caaatcacta
    11461 aacccaacta cttccacgtt ccccagatgc gcggttgcct gctgtgccag cgccacacgc
    11521 tcttccaggg taaacatcgg ttttttactg gggctggcgg caatcgccag aataacgtga
    11581 tcgaacattt gcgtggcgcg cgtcacgata tcgatatgac cattggtaat gggatcgaaa
    11641 gtacccggat aaatcgcccg tttttgcata acaacctcaa tgcgttttcg gtggcaggta
    11701 aggttccagc agttgaagca ggcgctgtag cgcgccctgg ttttgataca gtacttcaac
    11761 ggcatgacgg ccatagaaac tacggtaatc ggcgtcggtg agtaaagagg aaacctcttt
    11821 tgcaagcgta gtggcatcgg taacggtaat cagcccgctt gcctgctcca gccgcgcgca
    11881 aatgtcttta aaattaaaag tatgcggccc catcaatacc ggaatagcgt gtgcggcagc
    11941 ttccagcgga ttatgcccac cacgttcaac cagtgaaccg ccaacaaagg cgagatcggc
    12001 aatgccgtac agtaacatca actcgcccat cgtatcgcca accacaacct gcgtgctggt
    12061 ggaggggact tcccctgaag agcgtgtgat atagcttagt ccagcctggc ggacaaggtt
    12121 aatcgcatcc gggaagcgtt ctggatgacg gggtaccagg atgagcaata aattcgggaa
    12181 ttgctgtaac aatacctgat gtgcggcgat caccacactc tcttcacctt cgtgagtgct
    12241 ggtggcaatc cataccgggc ggtgtggtgc ccactggcgg cgcagcgtca cggctttagc
    12301 agccaactgc ggcgttacag aaatatcgaa tttcaggcta ccggtaacgg ttacctggtt
    12361 attttttgcg cccagcgcca caaaacgtgc accatcttct tcattttggg cggcaatcag
    12421 cgtaatacga cgcagcaagc gacggacgaa tttacccagt ttggcataac ctgcggccga
    12481 gcgggcagag agtcgcgcgt tcgcgatcac cagcggaatt ttacgtttat gtagcgccgc
    12541 aatcaggtta ggccatagtt cggtttccat aatcaacacc agtttagggt cgactttatt
    12601 caggaaacga ttaagtgcat cgggcaggtc atacggcaga taaacgtgct gaacatcctt
    12661 cccgaaagcc gattgtacgc gctccgaacc ggttggcgtc atggttgtaa cggtaatcgg
    12721 taaatcagga taacgatgac gcagcgcgcg caccaacggg atcgccgcca gagtttcacc
    12781 gacggagacg gagtgcagca taatgccgcc tggttttagc ggatggcggt aaaaaccgta
    12841 acgttcaccc cagcgttttc gataggccgg agccttacgt ccgcgcaccc agagccgtat
    12901 ccagatcagc ggctgaataa ggtagagaag ggcggtgtaa agcaattcga gcatagtaaa
    12961 tagctgactt atggatgtgc tggggattct atgtatttag ctgtggcttt accattactt
    13021 ttcccgtttt tgacttaaat agcaatttac ccgccaattc ttgctgaaca tctatacttt
    13081 ttttgtattt ttatgcgatt cattgatttc tgctgtacgt gcaggaagga atcgcagagc
    13141 aacagtgaaa gaatatggtt gctaatgatt ttatttgatt gatttcttta ttgaaaatca
    13201 acaatatgct taaaaatcat tcgggtcgga ttgccgcgaa agaagtgata cactagcgcg
    13261 tcaatatcag tgcggtatca gtattcaggt agctgttgag cctggggcgg tagcgtgctt
    13321 tttctgcttc accttaatcg gataatctca acaaaaagag ttacttgtgg ataagctatt
    13381 tcgaagaatt ttgctcatta agatgcgttt tcatggggat atgttattaa ctactcccgt
    13441 cattagttcg ctgaaaaaaa attaccctga cgcaaaaatc gatgtgctgc tttatcagga
    13501 caccatcccg atcctgtctg aaaatccaga gattaacgcg ctctacggca taaaaaataa
    13561 aaaagcaaaa gcctcagaaa aaattgccaa cttttttcat ctcatcaagg tattacgtgc
    13621 caataagtat gaccttatcg tcaatctcac cgatcaatgg atggttgcta tactggttcg
    13681 cttattaaat gcccgtgtga aaatttccca ggattatcat catcggcagt ctgctttttg
    13741 gcgtaaaagt ttcacccatt tggtgccgtt gcagggtgga aatgtggtgg aaagtaactt
    13801 atccgtgctg accccattgc gagttgattc gttggtgaag cagacaacca tgagttaccc
    13861 gcctgcaagc tggaaacgta tgcgtcgcga acttgatcat gctggtgttg gacaaaatta
    13921 tgtggttatc caacctacgg cgcggcaaat cttcaaatgc tgggacaacg ccaagttttc
    13981 cgctgtgatt gatgccttac atgctcgtgg ttatgaagtt gttctgacgt ccggcccaga
    14041 taaagacgat ctggcctgcg tcaatgaaat tgcgcaggga tgccagacgc caccagtaac
    14101 ggcgctggct ggaaaggtga ccttcccgga acttggtgcg ttaatcgatc atgcgcagct
    14161 gtttattggc gttgattccg caccggcgca tattgccgct gcagttaata cgccgctgat
    14221 atcgctgttt ggtgcgacag accatatttt ctggcgtccc tggtcaaata acatgattca
    14281 attctgggcg ggagattacc gggaaatgcc aacgcgcgat cagcgtgacc gaaatgagat
    14341 gtatctttcg gttattccgg cggcagatgt cattgctgct gtcgataaat tactgccctc
    14401 ctccacgaca ggtacgtcgt tatgatcgtt gctttttgtt tatataaata ttttcccttt
    14461 ggcggtttgc agcgcgattt tatgcgtatt gctcagacag tcgccgcccg aggtcatcat
    14521 gttcgggttt atacccagtc gtgggaaggc gaataccctg atgtatttga actgatcaaa
    14581 gtgccggtta aatcgcatac caatcacggg cgcaatgcgg agtattttgc ctgggtgcaa
    14641 aaacatttac gcgaacatcc cgtcgataaa gtcgttggat tcaacaaaat gccggggctg
    14701 gacgtttatt atgccgctga tgtttgttat gccgagaaag tcgcgcagga aaaaggcttt
    14761 ttctatcacc tgacgtcacg ttatcgccat tatgccgcct ttgagcgggc aaccttcgaa
    14821 cagggcaagc cgacacagct gctgatgctg acagataagc aaatcgccga tttccagaaa
    14881 cattatcaga ctgaagcgga gcgttttcat attctgccac cggggattta tcctgatcgt
    14941 aaatatagcc agcagccagc taatagccgt gaaatcttcc gtaagaagaa tggaataacc
    15001 gaacaacaat atttattgtt gcaggtcggt tcagacttca cgcgtaaagg tgtcgatcgt
    15061 tccattgaag cacttgcttc gttaccggat tcgctgcgcc acaacacatt gctatatgtt
    15121 gttggacagg ataaaccgcg aaaatttgag gcgctggcag aaaaacgcgg cgtgcgcagt
    15181 aatgttcact tcttctcggg gcgcaacgat gtctcggaat taatggcggc ggcggattta
    15241 ttactgcatc ctgcctacca ggaagcagcg ggaattgtgc tactggaagc gattactgca
    15301 ggattaccgg tactaacaac agccgtttgt ggctatgcgc attatattgt cgacgccaat
    15361 tgcggcgagg ctattgctga gccattccgc caggaaacat tgaatgagat tttacgcaaa
    15421 gcgttaacgc aatcttcatt gcgccaggcc tgggcggaaa atgcgcgaca ttatgctgat
    15481 acacaagatt tatacagtct gccagagaaa gcggcggata tcataacggg tggtctggat
    15541 ggttgaactt aaagagccgt ttgccacgtt atggcgcggt aaagatcctt ttgaggaagt
    15601 taaaaccttg cagggtgagg tttttcgtga actggaaact cgccgcactc tgcgatttga
    15661 aatggcgggc aaaagctatt ttctcaaatg gcatcgcggc acgaccctga aagagataat
    15721 caaaaattta ctctcattgc ggatgccagt attaggcgca gaccgcgaat ggagtgcgat
    15781 tcatcgactg cgggatgtcg gcgttgatac tatgtatggg gtggcatttg gcgaaaaagg
    15841 tattaatccg ctgagcagaa cctcgtttat tattaccgaa gatctgaccc caaccatcag
    15901 tctggaagat tactgtgctg actgggcgac caacccacca gatgttcgcg taaagcgtat
    15961 gcttattaag cgtgtcgcga cgatggtgcg cgatatgcat gctgcgggca ttaaccaccg
    16021 tgactgttat atctgtcatt tcctgctgca cttgcctttt tccggtaagg aagaggagtt
    16081 aaaaatttcg gtaattgacc tacaccgggc gcagcttcgc acgcgcgttc cacgtcgttg
    16141 gcgcgataaa gatcttatag ggctttattt ttcttcaatg aatattggcc tgactcagcg
    16201 ggatatctgg cggtttatga aagtgtattt tgccgccccg cttaaagaca ttctcaagca
    16261 ggaacaagga ctgctgtcgc aagcagaagc aaaagccaca aaaatcaggg aaagaacgat
    16321 tcgaaaatcg ttgtaatttc cggagagaaa tatgagtgcc cactatttta atccacaaga
    16381 gatgatcaat aagacaatca tcttcgatga aaggccagcg gcgtcagtgg catcatcatt
    16441 ccatgttgct tatggcattg ataaaaactt tctttttggt tgtggtgttt caatcacgtc
    16501 agttttgtta cataacaacg acgtgagttt tgttttccac gtttttattg atgatatccc
    16561 tgaagccgat atccagcgtt tagcccaatt ggcgaaaagc tatcgtacct gtatccagat
    16621 ccatctagta aattgtgaac ggcttaaggc attaccgacg accaaaaatt ggtctattgc
    16681 catgtatttc cgttttgtaa ttgcagatta ctttattgat caacaagata agatccttta
    16741 cctggatgct gatatcgcct gtcagggaaa cttaaagccg ctgataacaa tggatcttgc
    16801 caataacgtt gctgctgttg ttactgaacg cgatgctaac tggtggtcgt tacggggtca
    16861 aagtctgcag tgtaatgaac ttgaaaaggg ctactttaat tcaggtgtcc tgttaattaa
    16921 tacactagcg tgggcgcagg agtccgtttc tgctaaagcg atgtcgatgc ttgctgataa
    16981 agccatcgtt tcccgtttca cctatatgga tcaagatatc cttaatctta tcctgttagg
    17041 gaaagttaaa ttcattgatg ctaaatacaa tacgcaattt agtttaaatt atgaattaaa
    17101 aaaatcattt gtttgtccaa ttaatgatga aaccgtatta attcattatg tcggcccgac
    17161 aaaaccctgg cattactggg ccggttatcc aagtgcgcaa ccttttatca aagccaaaga
    17221 agcatcgccc tggaaaaatg aaccgttaat gcggccagtt aactcaaact atgctcgtta
    17281 ttgcgccaag cataatttta aacaaaataa accaattaac gggataatga attatattta
    17341 ttatttttat ttaaagataa taaaatgaaa ttgagaaaag ttaaatgaat gaatttataa
    17401 aagaacggtt ttcgtattta gcagataata aaaaagaaaa cgccccagag ctaaatgttt
    17461 cctacggtat cgataagaat tttttgtatg gtgctggcgt ttcaatttct tccgttttga
    17521 ttaataattc agatattaat tttgtctttc atgttttcac tgattatgtg gatgatgatt
    17581 atttaaagtc atttaatgaa acagcaaaac aatttaatac ctcaattatt gtatatttaa
    17641 ttgaccccaa atactttgct gatctgccga cgtcacagtt ttggtcatac gcgacatact
    17701 tcagggtatt gtcttttgaa tatctgagtg aaagtatttc cacactgctg tatctggatg
    17761 ccgatgttgt ttgtaaagga agcctgaaac ctctcacaga aattatattt aaagatgagt
    17821 ttgctgcggt cattcctgac aatgatagta ctcaggcggc atgtgcaaaa cgcctcaaca
    17881 ttcccgaaat gaatggacgt tatttcaatg caggcgttat ctatgtcaat cttaaaaaat
    17941 ggcatgaagc aaatttgaca ccgtatttac tcaaactttt acgaggggaa actaaatatg
    18001 gctctcttaa atatttagat caggatgcgt tgaatatcgc atttaatatg aataatatct
    18061 accttgcgaa ggattttgat actatttata ccctgaaaaa cgaactttat gatcgtagtc
    18121 atcgaaagta tcagcaaacc attaccgata aaacagtgtt gattcactat acagggataa
    18181 ctaaaccatg gcatagctgg gctggatatc cgtctgcatc atactttaat atcgcgcgtg
    18241 aacaatctcc ctggaagaaa tatcctctta aagaggcgcg gactgttgca gaaatgcaga
    18301 aacaatataa gcatctgttt gcccatggtg agtatataaa aggcataact tcattaatta
    18361 agtacaagct taagaaataa attactctta tgattacaag tatacgttat cgcggcttct
    18421 cattttatta caaagataat gataataaat ataaagaaat ctttgatgaa atactggcct
    18481 ataattttaa aacagtaaaa gtattatgta atattgatga taccaaagta tcgttgattg
    18541 atacgaaata tggtcgctat gtttttaaag tctttgctcc gaaaacgaaa aggaatgaac
    18601 gttttttaaa atcttttgta aaaggtgact actatcagaa tttgatcgtt gaaaccgatc
    18661 gtgtcagaag tgcaggtctt acttttccta atgattttta ttttctggct gagcgtaaaa
    18721 ttttcaacta tgccagtgtt tttatcatgc ttatcgagta tgtcgagggc gtagagctca
    18781 atgatatgcc gattattcca gaaaatgtta aagcagaaat taaggcttcg atggaaaaat
    18841 tgcatgcctt aaatatgcta tcgggcgacc cacaccgggg taactttatc gttagtaaag
    18901 atggtgtcag gataattgac ctatccggta aaagctgtac ggcagagcgt aaagctcgtg
    18961 atcgtctggc catggaacgt cacctgggta ttgccaacga aataaaagat tatggctact
    19021 attcagtaat ctacagaacc aaactacgca aatttattaa aaaattaaaa ggcaaagcgt
    19081 aaaccacaca gtcaaaacgg aaccaacatg gatttattag cagagagtat tactgaagtc
    19141 gctgtctctg gggaaattgc taacaccgat cgtgtgttaa atatcgctta cggtattgac
    19201 cgtaactttt tatttggtgc ggcagtatct atgcaatcag ttgttatgca taacccggac
    19261 cttgcggtta agtttcatct ctttaccgac tacattgatg aagattatct acaacgtgtt
    19321 aatgctttta ccagcaaaaa tgctaacgtt gaagtaataa tttataaagt ctccaatgcc
    19381 tttattgata tcttccccag cctgaaacag tggtcttatg caacattctt ccgtttagtt
    19441 gcgttccagt atttgagtga aactattgaa aatctgttat atatcgatgc tgatgtcatc
    19501 tgtaaaggct cattagctgg attgcttgat attaattttg atgaggataa gttcgcagct
    19561 gttattaaag atgtgccttt tatgcaggaa aaaccagcga agcgtctggc tatagaggga
    19621 cttccaggga attatttcaa cgccggtgta gtatatctgc agcttgaagc atgggcgaaa
    19681 aatgatttta tgaataaagc cattgctatg ctggcaagtg acccgcagca cacgaagtat
    19741 aaatgccttg atcaggatat tttaaatatt ctgttctttg gtcattgtaa ttttattagc
    19801 ggcgattatg attgctttta tggcattgac tatgagttaa aaaataaaag cgatgaagat
    19861 tataaaaaga ccattaccga tgatactaag ctgattcatt atgttggcgt aacgaagccc
    19921 tggaacgact ggacgaatta tccctgccag aagtatttta atgaggctta tcaggcttct
    19981 tgctggaatg atgtggcgtt tattccagcc acgaatgaaa agcagtatca agtgaaatat
    20041 caacatgcaa agaaaaatgg tgatacgttt aacgctttta tttacttcat taaatttaaa
    20101 ttaaataagt ataaaagaaa actatttgga taataataaa aatcatggat gtctgagtta
    20161 caataataat taaggatgag tactatcata ttttattagt tcaggcaaga gaacacgatg
    20221 agcaatgatt accctttagt atctataata ataccgacgt ataattcatc tgattacatt
    20281 actgaaactc taacgaaatt agaaaaacaa acttacccaa attttgaaat tgttattgtt
    20341 aatgatggtt ctaaagataa cacatcaaac gttttgagag agtatgggtt aacccactct
    20401 cgattaatta ttgtcaataa agaaaatggc ggtgtttcgt ctgccaggaa tacaggtatc
    20461 cgcaaggcgc aaggacagtt tatatgtttt atggatgatg atgatgagat agatcctaac
    20521 tatctgctga agatgtatac cagacaacat gagacgggag gggatgcgat ttattgtggg
    20581 ctttatggcc attatataaa agatggtgtt acttactcac ctataaatac agagtttaat
    20641 gaaggatctt tacttttcga ctttttttat aaaaaggtta gattccatat agggtgcttg
    20701 tttataagaa aacaacttct ggaagagaat aatctttttt ttgatgaaga tttacgacta
    20761 ggagaagatc ttgattttat ctatcgactg ctaattacat gcgatatgta tgcggttcca
    20821 tattatatgt ataagcataa ctatagagaa aattccttaa tgaactcatg tagaaccatc
    20881 actcattatc gacatgagtc atttgcgcac gaaagaatct actcttctgt gatgcagtta
    20941 tacaaaggta accggaaaga agaaattcat acattattga gtcaaaatag agcttatcat
    21001 aaaactcgtt atttatggaa tgttctactt aatggtgatt ttaaactatt gaatcaatta
    21061 gttgaaagca atgaaaaaga attaagagat tgtaatcttc ctggcaagag agataagaga
    21121 cgagcaaaaa tattagcatc aaaaaattat attctctgga ggatggtaag actggtaaat
    21181 agaaaaaaga ataaacgtta gatcttgcat taaataaccc atcctcgtag cataggttga
    21241 aattatatgt cgttttgttg gaatgaaatt aattctggta taaagtcttt aattcttata
    21301 ttatgtattt tttctttaat gactttgtct ttatgggatg atgttgcaac aaagtttctt
    21361 catgcagctg gaattatatc tgcattgtat tttcttgcga caccaaaaaa aacaataact
    21421 aataatccta ctttgttaat tttcatctca ttatgtcttt tgggtatcgt aaatatcatc
    21481 tggtattcac attataaagt ttcaggctct gtttatacca atgcatatcg tggcccaatg
    21541 gaaactggaa aaattgcctt gtgtagcgcc tttattttct tagttctttt tgctaaaaat
    21601 gagatgagaa caaaaataaa atttgggaaa ctaattctgt tcgcatccct ggcaacgcag
    21661 ttactttttt ttgcgcatgc catgtggcaa catttctatt taaacgtcga ccgtgttgca
    21721 ttatcagctt cccacgctac aacagcaggc tacatcatcc tttttccttc tttactggca
    21781 tcaattctca ttttaaaatc cgactttaga cataaaacaa cattatatac aattaacttc
    21841 atgcttagct tatgtgctgt catagtaact gagacgcgtg cagccatatt agtgtttcca
    21901 ttctttgcgt taatattaat cgtaatggat agttatatta ataagcgaat taattataag
    21961 ttatattgtt ttattgcgat tgcattatta gcaggtgtat tttcttttaa agatacattg
    22021 cttatgagaa tgaatgactt aaataacgat ttagttaatt attcgcatga taacaccaga
    22081 acttcagtcg gtgcccgtct ggcaatgtat gaagttggct taaaaacata ttctccaata
    22141 ggacaatcac tggaaaaacg tgcagaaaaa atacatgagc tagaagaaaa agagcctaga
    22201 ttgagtggcg ctttaccttt tgtagattct catttgcata acgatctcat agatacgtta
    22261 tcaacgcgtg gtattcctgg agttgtatta acaattttag cattttcagc aatactcata
    22321 tatgccttaa gaactgctaa agaaccttat attttaatct tgcttttttc actactggta
    22381 gtaggcctaa gtgatgtaat actcttttct aaaccggttc cgactgctgt gtttgtcacc
    22441 ataatattgc tttgtgctta ttttaaagca caatcagacc aatatttatt agataagtaa
    22501 tgaatatatg aaataaaatc agcaaggagg ctgattttat ttcaaaatta tctattaagg
    22561 ggttgtaatt taaatcatag cagcgttttc ttcaatgaat cgcttaacag catttgctgt
    22621 caattgagac aactcattcc ccggagccct acaaaccatt tgattcttcc catacccacc
    22681 aattaatccc ggatcggttg gtccataaac cgtgatattg ggtctatcca gtgccgccgt
    22741 taaatggctt aaccccgtat ccaccgacac tacaaattta gccccggcca gcacgcgggc
    22801 aacgccttcc agactcatct tcggcaatac ttcaacataa tcaaatcctt ccgccagtcg
    22861 tttcgcccgt tcttcctcat gcggcgcgcc ccacggaagt ttaatccgta ttcctgaatc
    22921 agccagtaaa ccaatcaatt ctcgccagtg ttcttccggc cagtgtttat catcacgggt
    22981 cgtcgcatga agaaatacgg catattcgcc agcatctgta ggcaggttcg tcagaaaatg
    23041 ctgtgcgata gcataatcgc cctgggtttg cggtttgcta tagcccaaac ttttggcaaa
    23101 cagttcgcgg gtgcgttcta cggcgtgctg ctgttttgca atatgatgct tacaattgta
    23161 aaacaggctg gctaaaggtt cgcgagcggt ttgccagtcc aatccatgct ttacgccatg
    23221 cgccagacgt gtcaccagtg ccgcgctttt taccagcccc tgagcgtcga taactgcgtc
    23281 atagttctct gcttgtagcg cttcacgaaa cgctttgcgt tcagctttta tgggggccga
    23341 gaaccaggct ttacgccagc gacgtattgc cacaggaata actcgctcaa cggcagcgtg
    23401 ccaggaagga atctgtgcga acccttcttc caccacccag tcaaacttaa tccctgggat
    23461 tgcctgctgg gcatcagtga gtgcgggcaa cgtatggaga acatcgccca tcgacgatgt
    23521 tttaacgatc aaaacccgca tccgtcaggc ttcctcttgt aacaatagcg tgttgagttc
    23581 ttccagtacg cgctggggag taatgtcgat caagctctgg tgataaccct ccgcagcgtc
    23641 acctttacgc actttgtgat agccggtaat cagacggatc acgcgcgctt tatgggatag
    23701 cggcggtgtg aagtccgggc tacttggacc atacagggca accagcggac gattgagcgc
    23761 cgccgcaacg tgcatcaggc cagaatcgtt agtgacaatg gctttacagg ctgcaatcag
    23821 gataaccgct tgatcaagct gtgtttcccc cgccaggttc cgacaccatg cctgctgctc
    23881 ggtattcaaa gcggcaagaa tctcattgcc cgcttcatga tctttcgcag agccaaacag
    23941 aaccacctga taaccttcat caatcagctg ctttgccagt tccgcatagt ggtagtgtgg
    24001 ccagcgtttt gccggaccaa actccgcacc cgggcaaaag ccaatcatcg gacgttctga
    24061 tgaaagcgaa aattgattac aggtatatga tttttcacct tcgctcacct gcaactgcgg
    24121 ccataacaat ggctgcggca gatcttgtgc ggtacgcata atgcctttgt cataggccag
    24181 cgcgacatag cgttccacca ttagcggcca ggcttcttta tcgagcacgc gtacatcgtt
    24241 gagtaaaccg tagcgcatct cgccgcgcca gccggtgcga tgaggaatac ccgcgaagaa
    24301 aggcactaat gcagatttga aggagttggg taagacgtag gcgcggtcgt agcgcttttc
    24361 acgcaggcta tgacccagtt tgcggcgttc gccgatttcc agcgctccgt gaccgagagg
    24421 catagggata gcttcgttaa cttccggcat ccgcgataat aatggacggc accatgccgg
    24481 tgccatcaca tcgattatcg cctggggata gcgcgcctgg agcgtgcgat agagactttg
    24541 cgacatcatc atgtcgccaa cccaagacgg gccgatcacc agtattttca tgcagagctc
    24601 ttatgcgtcg cgattcagcc aggccatgta ttccgttaca ccttcagcaa cggttttgaa
    24661 cggtttgtcg taacccgccg cgcgcagatt tgtcagatct gcctgagtga acgcctggta
    24721 gcggcctttc agcttatccg ggaacggaat gtattcgatc tggcctttct tgtgataagc
    24781 cagagtagca tctgctacgg cctggaagga ttccgcacga ccagtaccga ggttgaagat
    24841 accggaaacg ccattttcca ggaaccacag gtttacatct gccacgtcgc ccacatagac
    24901 gaagtcacgt ttgaagttct cgctaccttc aaacagcttc ggtgattcac cgttgttaag
    24961 ctgagtgttg agatggaaag cgacgctcgc catgctgcct ttatggcctt cacgcggtcc
    25021 ataaacgttg aaatagcgga agccaacaat ctgcgagttc gcttcaggca ggatttggcg
    25081 aacatattca tcaaacagga attttgagta accgtagacg ttcaacggtt tttcgtactc
    25141 gcgggattca ataaagtcgg aggtgcgtcc gccgtaggtg gctgcggaag aagcgtacag
    25201 gaacgggatt tcgcgctcca ggcagtagtg cagcagctcc ttggagtatt gatagttgtt
    25261 atccatcata tacttgccgt cccactcggt ggtggaagag catgcgcctt cgtggaaaat
    25321 cgcttcgaca tcgccgaact cttcgccagc cataatctgg atcaggaagt cttccttatc
    25381 catatagtcc gcgatatcca gatccaccag gttcacaaac ttggtgccgt ctttcaggtt
    25441 gtccaccacc agaatatcgg tgatgccttt atcattcagg gccttaacga tgttgctgcc
    25501 gataaagccc gcgccgccgg taacgatgat cataactgta accttcgaat tatggagtca
    25561 gagacaatct cagacacgaa tacttctatc atatcactat acggcgttgg cttcagccat
    25621 tcgccaaaag tgagattgat ggacgttatt tatcctgaat tttgcagaag tgttaacgcg
    25681 tcatctcgtc gcgacgtata agtttgggta atatgtgctg gaatttgccc tgtctggaga
    25741 atcgcaatgc gtggagaatt ttatcagcag ttaaccaacg atctggaaac cgcacgggcg
    25801 gaagggttgt ttaaagaaga gcgcattatt acgtctgcgc agcaagcaga tatcactgtg
    25861 gctgatggaa gccacgtcat taacttttgt gccaacaact atctcgggct ggcgaatcat
    25921 ccggatctga ttgcggcagc aaaggcggga atggattctc acggtttcgg catggcttcg
    25981 gtgcgtttta tttgcggcac tcaggacagc cataaagagc ttgaacaaaa actggcggca
    26041 ttcctgggga tggaagatgc gattctctac tcttcctgct ttgatgctaa cggtggactg
    26101 tttgaaacgc tgctgggtgc ggaagacgcc attatctccg acgcactgaa ccacgcgtct
    26161 attattgatg gtgtgcgtct gtgcaaagct aaacgctatc gctatgccaa caacgatatg
    26221 caggagctgg aagcacgtct gaaagaagcg cgtgaagccg gtgcgcgtca tgtgctgatt
    26281 gccaccgatg gtgtgttctc aatggacggc gtgattgcca acctgaaagg cgtttgcgat
    26341 ctggcagata aatacgatgc cttggtgatg gtggatgact cccacgcggt cggttttgtc
    26401 ggtgaaaatg gtcgtggttc ccatgaatac tgcgatgtga tgggccgggt cgatattatc
    26461 accggtacgc ttggtaaagc gctgggcggg gcttctggtg gttataccgc ggcgcgcaaa
    26521 gaggtggttg agtggctgcg ccagcgttct cgtccgtacc tgttctccaa ctcgctggca
    26581 ccggccattg ttgccgcgtc catcaaagta ctggagatgg tcgaagcggg cagcgaactg
    26641 cgtgaccgtc tgtgggcgaa cgcgcgtcag ttccgtgagc aaatgtcggc ggcgggcttt
    26701 accctggcgg gagccgatca cgccattatt ccggtcatgc ttggtgatgc ggtagtggcg
    26761 cagaaatttg cccgagagct gcaaaaagag ggcatttacg tcaccggttt cttctatccg
    26821 gtcgttccga aaggtcaggc gcgtattcgt acccagatgt ctgcggcgca tacccctgag
    26881 caaattacgc gtgcagtaga agcattcacg cgtattggta aacaactggg cgttatcgcc
    26941 tgaggatgtg agatgaaagc gttatccaaa ctgaaagcgg aagagggcat ctggatgacc
    27001 gacgtccctg taccggaact cgggcataac gatctgctga ttaaaatccg taaaacagcc
    27061 atctgcggga ctgacgttca catctataac tgggatgagt ggtcgcaaaa aaccatcccg
    27121 gtgccgatgg tcgtaggcca tgaatatgtc ggtgaagtgg taggtattgg tcaggaagtg
    27181 aaaggcttca aaatcggcga tcgcgtttct ggcgaaggcc atatcacctg cggtcactgt
    27241 cgcaactgtc gtggcggtcg tacccatctg tgccgcaaca cgatcggcgt cggcgttaac
    27301 cgtccgggct gcttcgccga atatctggtg atcccggcgt tcaacgcctt caaaatccca
    27361 gacaatatct ccgacgacct ggcttccatt tttgatccct tcggtaacgc cgtgcatacc
    27421 gcgctgtcgt tcgatctggt tggcgaggat gtgctggtct ctggtgcagg tccgataggt
    27481 attatggctg cggcggtggc gaaacacgtt ggtgcacgca atgtggtgat cactgatgtt
    27541 aacgaatacc gccttgagct ggcgcgcaaa atgggtatca cccgtgcggt taacgtcgcg
    27601 aaagaaaacc ttaatgatgt gatggctgaa ctgggcatga ccgaaggctt tgatgtcggt
    27661 ctggaaatgt ccggtgcgcc gccagcgttt cgtaccatgc ttgacaccat gaaccacggc
    27721 ggccgtattg cgatgctggg tattccgccg tctgatatgt ctatcgactg gaccaaagtg
    27781 atctttaaag gcttgttcat taaaggtatt tacggtcgtg agatgtttga aacctggtac
    27841 aagatggcgg cgctgattca gtctggcctc gatctctcgc cgatcattac ccatcgtttc
    27901 tctatcgatg atttccagaa gggctttgac gctatgcgtt cgggccagtc cgggaaagta
    27961 attctgagct gggattaaca cgaacaaggg ctggtattcc agccctttta tctggggata
    28021 atctgttgaa tatgtaaaat cctgtcagtg taataaagag ttcgtaattg cgctgatctc
    28081 ttatatagct gttctcatta tctctctacc ctgaagtgac tctctcacct gtaaaaataa
    28141 tatctcacag gcttaatagt ttcttaatac aaagcctgta aaacgtcagg ataacttcag
    28201 aggtcgtcgg taatttatga tgaacagcac caataaactt agtgttatta ttccgttata
    28261 taatgcgggc gatgatttcc gcacttgtat ggaatcttta attacgcaaa cctagactgc
    28321 tctggaaatc attattatta acgatggttc aacggataat tctgttgaaa tagcaaagca
    28381 ttacgcagaa aactatccgc acgttcgttt gttgcatcag gcgaatgctg gcgcatcggt
    28441 agcgcgtaat cgtgggatcg aggtggcgac gggcaaatat gtcgcttttg tcgatgctga
    28501 cgatgaggtc tatcccacca tgtacgaaac gctgatgacc atggcgttag aggacgacct
    28561 cgacgtggcg cagtgcaacg ctgactggtc ttttcgtgaa acgggagaaa cctggcaatc
    28621 catccccagc gatcgccttc gctcaaccgg cgtattaacc ggcccggact ggctgcggat
    28681 ggggctttct tcgcgccgtt ggactcacgt tgtctggatg ggggtttatc gccgtgatgt
    28741 tattgttaaa aataacatta aatttattgc cggattacat catcaggata ttgtctggac
    28801 gacagaattc atgtttaacg cgctgcgtgc gcgatatacc gagcaatcat tatataaata
    28861 ttatctgcat aatacgtcag tgagtcggtt gcatagacaa gggaataaaa accttaatta
    28921 tcaacgtcac tatattaaga ttacccgcct gctggagaaa ttaaatcgaa attatgccga
    28981 caaaattacg atttatccgg aatttcatca gcaaataact tacgaagcat tgcgtgtttg
    29041 ccatgcggtg cgcaaagagc cggatattct tacccgccaa cggatgattg ccgagatatt
    29101 tacttccggt atgtataagc gcctgattac caatgtgcgc agcgtgaagg tcggttacca
    29161 ggcgttactg tggtctttcc gcttatggca atggcgcgac aaaacgcggt cgcaccatcg
    29221 cattacgcgt agcgccttta atttgcgcta gcgttaaact tcgccgcttc gggctgtttg
    29281 ccccagcctt gccactgatg ctggaagtaa acgatcagcg tgctctggct gatgctttcg
    29341 cttaatactt caaagaagcg attagcataa acaggttcta tcggtttctt cggtttgcac
    29401 agcttcacgc cacggaacgg attacgcggt gcatcaggca cagcgttttt cggcggtgta
    29461 gaagtatcaa cctgcggttc attcagcaag ctgctggctt tcaccagcgt aatgtctggc
    29521 ggcaggttat aaaccatctg ttgtagcacg cgtaccgtta aagggtgagg atgcccaatg
    29581 gcaatggttg aaccgttgcg acgcgccaga tcaattgcgc gattaaattg cacacggatg
    29641 tccgcttcgt tctgcgaatc gtcgaggaac accttccgtt taataacctt cacgccagta
    29701 ccttgcgcgg cgcgcatcgc ctgggtatta ccgatggtta cgctgtcgag gaagtaaaga
    29761 ttgtaacgct ccagtgcctg catcactttc tgcataccaa acaggttaga ggtcatcttg
    29821 ctacccatgt ggttgttgat ccccacggca tagggcacgt tattgaccgc actacgaata
    29881 atgcgctcaa tttcgtcgct gctcatctcc gggcgcagcg tatttttctc cagcggctgt
    29941 ttactcagtg gtgccatcgg gagatgaatc aacacttcgt gcccgctgtt atgcgctttg
    30001 gtcgccatct ctctggcgtg cggtgaatcg ggtaatacag cgacggagat agcggagggc
    30061 atcgccagca cctggttttc gttgtgcggg cgatacccaa aatcatcaat gacgatggca
    30121 agtttgccag caagtacggg ggaggagagc gccaacagag cggcaaatgc aagaacgtta
    30181 cgacgaaatg gaaacaaaac ttatcttccc aaccacggct gtggattgac cgcctgaccc
    30241 tggcggcgaa tttcgaaata gagtgaaggc cgaccctgac cgccactgct gcccaccagt
    30301 gcaattggct ggcccgcgcg aacctgcgaa ccaacgctca ccagtgcgct ctgattatag
    30361 ccgtaaagac tcatgtcgcc tttaccgtgc tcaaccacca ccaccagacc gtaaccttgc
    30421 agccagtcag ccagaatcac tcgaccgtcg gcaattgctt taacttcagt accttcagag
    30481 gcaccgataa ccatcccttt ccagcgtaat tcaccctgta gctgctcgcc atagcgatgc
    30541 agcgtcggcc cgcgaaccgg ccagaatgcc tgaccacgcg gcgcacccag accaccagtt
    30601 cgggacatca gcgatttttc gctttcggtc ggtttgtagg tggtgccttt gcgcgtcgct
    30661 tctttctggc ggtcgcgaac cgcctgggcc tcacgcgctt ctcgttcagc acgcgctttc
    30721 gccgcggctt ccgcacgggc aatgctgtta cgcagacggg attcgttggc acgcagttca
    30781 cttagctgct gctgaccttg ctggatggaa gattccagcc ccgccagcgt ctttttacgc
    30841 tcgttcagag cctgagtcag tttcgcctgt tgggcgcgct gttcatataa cagcgtttgt
    30901 tgctcgcttt gtttctcttc cagttcagca cgctgcatgg cgacttcttc acgcgtttgc
    30961 ttcaactggg caatggtttc ttgtcgcgcc tggttgagat agccgaaata agcctgtaaa
    31021 cgctgtccac actggctttc ttcaccgctg agaattagct gaataccggt atgctcgccc
    31081 tgacggaatg cggcatccag ttgtgcggcg aggctgcgct cctgggcggc tttttgctgc
    31141 tccagtttgg caatcgacgc gttcatctca tcaatctgtt tattgagttg attgagcgtg
    31201 ttttgcgttt cgcgcagctt acgggtggct tcagagatcg cttcttcctg ctttttcaat
    31261 tgtgcgagca ggcttgcgcg ttgttgttgc ttttggcgta ccgcgcgctc ttttgcggcg
    31321 atatcggcct gaatagattt gagttggtca cgctcatccg cgtgggcgga aaaggcgcac
    31381 aacaatacgc cagcgctaag aacgctggcg tagatgatgg gcctgattgc aaacctgcgc
    31441 ggtttcacgg cccgtgtcat ggtattaatc gcctttcccc tcatggggag ggattattcc
    31501 acgatgaaca gcggcttacc agtcatctct tgcgggattt ccatacccat cagcgacaac
    31561 atggtcggcg cgatgtcaga aagtttgccg cctgcaaccg ctttcacgtt cttatcacca
    31621 acgtaaatca gcggaactgg caggttggtg tgtgccgtgt gtgcctgacc cgttgccgga
    31681 tcgcgcatct gttcagcgtt accgtggtca gcggtgatca gcagttgtcc acccacggac
    31741 tcaaccgctt tcgcgacttc ttccacgcag tgatccagtg cttcaaccgc tttaaccgcc
    31801 gcttccatca ccccggtgtg acctaccatg tcgccgttcg gatagttaca gataatggtg
    31861 tcgtatttgc cgctcttgat ggccgcaacc agtttttcgg tcagctctgc ggagctcatt
    31921 tccggttgca gatcgtaggt agccactttc ggtgagttga tcagaatgcg atcttcgcct
    31981 ttgaatgact cttctacgcc accgttgaag aagaaagtaa cgtgggcata tttttcggtt
    32041 tcggaaatac gcaactgagt tttgtcgttt ttcgccatcc actcgccgaa ggtgttaacc
    32101 agggaagcgg gtgggtaagc aaccgcagtt ttgatgtcag cggcgtattc ggtcagcatc
    32161 acgaaatcga cgttaaccac tttcttacgc gcgaagccgt cgaaatctgc gtttacgaat
    32221 gcacgagtga tttcgcgcgc gcggtcagca cggaagttca tgaaaatcag cgcatcgccg
    32281 tcttccattg ccgcatctgg ctgaccttct gcacgaataa cggtcgcttt cacgaactcg
    32341 tcgttttcgt cgcgagcata agctgcctgc aaaccagcaa cggcagtatc ggcctggaac
    32401 tcaccctgcg ccagagtcag caggtcatag gctttttcaa cgcgatccca acggttatcg
    32461 cggtccatcg cgtagtaacg accaatgatg gacgctacgc ggcctttacc cagcgcagca
    32521 aatttctctt caaatttttt cagcgaggat tcagcactgc gcggcggggt gtcgcgaccg
    32581 tcaaggaatg cgtgcaggta gattttttct gcgccgcgtt cagctgccag ttctaccatc
    32641 gccatgatgt gatcttcgtg gctgtgtacg ccgcctgcgg agagcagacc cataatgtgt
    32701 actgctttac ctgcgttttt cgctttatct accgcgccag tcagcactgg attagcaaag
    32761 aaagcgcgat ctttgatttc aacgtccaga cgagtcaggt cctgatacac gatgcggccg
    32821 gcacccaggt taacgtggcc tacttcggag ttacccatct gacggtcagg cagaccgact
    32881 tccagaccgg aagcgtcgat cagggtatgc ggacgattgg cccacagtgc atccattacc
    32941 ggggttttag cactaaaaat ggcgttatcc tgctgttctt cacgatagcc atagccatcc
    33001 agaatcacca gtaccatagg ttttttagaa accgacattg cgacaacctc atactcaaga
    33061 gtcaaaattt gcgtaatttt actacagctg aatcgataaa atagcctctg aagatcaaaa
    33121 tatacgaccg ctcgcggcaa gccgatgcca gatttgcgct tttttttcgt cgtgccccgc
    33181 aaaattgcat tccagttaac gcgctggctg tatttgccgc accgcgcagg tatactcctt
    33241 tcctggtttt tttaatcact acgtcgggag ttgttacccc ccatgcaaga aattatgcaa
    33301 tttgttggcc gtcatcccat actgagtatc gcttggatcg cgttactggt ggcggttctt
    33361 gtgactacgt ttaagagcct gacctcgaaa gtgaaggtga ttactcgtgg tgaagctacg
    33421 cgtctgatca acaaagaaga cgctgtggtt gtggatttac gtcagcgtga tgacttccgt
    33481 aaaggccata tcgcaggttc tatcaatctg ttgccgagcg aaatcaaagc caacaatgtt
    33541 ggtgagcttg agaagcacaa agacaaaccg gttatcgtgg tagacggttc tggcatgcag
    33601 tgccaggagc ctgcaaacgc gctgacgaaa gctggttttg cgcaagtatt cgtgctgaaa
    33661 gagggcgttg ctggctgggc tggcgaaaac ttgcctctgg tgcgcggcaa ataatttacc
    33721 tgtcgtcttt catgatgcag gccgttagct gcaattaggc ccccagcgac ttattgttat
    33781 aagcttctgg ggacactcca ttttgcagcc gccctgcaac atgaaatttc agggtaaaaa
    33841 aacaggagct aaattatggc caatgttgaa atctatacca aagaaacctg cccgtattgc
    33901 catcgtgcaa aagcactgct gagcagcaag ggcgtgagtt ttcaggagtt gccgatcgat
    33961 ggcaatgccg ccaagcgtga agagatgatc aaacgcagcg gtcgcaccac ggttccccag
    34021 atttttattg acgcacagca cattggcggc tgtgatgact tgtatgcatt ggatgcacgt
    34081 ggtggactgg atcccctgct gaaataacgt gtgaacgttg gcattacatt gcgcagtatt
    34141 taaggacaac acttaagggt tttctacaca tgtcagaaca aaacaacact gaaatgactt
    34201 tccagatcca acgtatttat accaaggata tctctttcga agcgccgaac gcgccgcacg
    34261 ttttccagaa agattggcaa ccagaagtta aacttgatct ggatacggca tcttcccaac
    34321 tggcagatga cgtatacgaa gtggtactgc gtgttaccgt aacggcctcc ctgggcgaag
    34381 aaaccgcgtt cctgtgtgaa gttcagcagg gcggtatttt ctccatcgcg ggtatcgaag
    34441 gcacccagat ggcacattgc ctgggcgcat actgcccgaa cattctgttc ccgtatgctc
    34501 gtgagtgcat caccagcatg gtatcccgcg gtacattccc gcaactgaac cttgcgccgg
    34561 ttaacttcga tgcgctgttc atgaactatt tgcagcagca ggctggcgaa ggtactgaag
    34621 aacatcagga tgcctgatga accaacgtaa tgcttcaatg actgtgatcg gtgccggctc
    34681 gtacggcacc gctcttgcca ttaccctggc aagaaatggc cacgaggttg tcctctgggg
    34741 ccatgaccct gaacatatcg caacgcttga acgcgaccgc tgtaacgccg cgtttctccc
    34801 cgatgtgcct tttcccgata cgctccatct tgaaagcgat ctcgccactg cgctggcagc
    34861 cagccgtaat attctcgtcg tcgtacccag ccatgtcttt ggtgaagtgc tgcgccagat
    34921 taaaccgctg atgcgtcctg atgcgcgtct ggtgtgggcg accaaagggc tggaagcgga
    34981 aaccggacgt ctgttacagg acgtggcgcg tgaggcctta ggcgatcaaa ttccgctggc
    35041 ggttatctct ggcccaacgt ttgcgaaaga actggcggca ggtttaccga cggctatttc
    35101 gttggcctct accgaccaga cctttgccga tgatctccaa caattgctgc actgtggcaa
    35161 aagtttccgc gtttacagca acccggattt catcggtgtg cagcttggcg gtgcggtgaa
    35221 aaacgtcatt gccattggcg cggggatgtc cgacggtatc ggttttggtg cgaatgcgcg
    35281 tacggcactg atcacccgtg ggctggctga aatgtcgcgt cttggcgcgg cgctgggtgc
    35341 cgagcctgcc acctttatgg gcatggcggg gctgggcgat cttgtgctta cctgtaccga
    35401 caaccagtcg cgtaaccgcc gttttggcat gatgctcggt cagggcatgg atgtacaaag
    35461 cgcgcaggag aagattggtc aggtggtgga aggctaccgc aatacgaaag aagtccgcga
    35521 actggcgcat cgcttcggcg tagaaatgcc aataaccgag gaaatttatc aagtattata
    35581 ttgcggaaaa aacgcgcgcg aggcagcatt gacgttatta ggtcgtgcac gcaaggacga
    35641 gcgcagcagc cactaacccc agggaacctt tgttaccgct atgacccggc ccgcgcagaa
    35701 cgggccggtc attatctcat cgtgtggagt aagtaatgtc gtgtgaagaa ctggaaattg
    35761 tctggaacaa tattaaagcc gaagccagaa cgctggcgga ctgtgagcca atgctggcca
    35821 gtttttacca cgcgacgcta ctcaagcacg aaaaccttgg tagtgcactg agctacatgc
    35881 tggcgaacaa gctgtcatcg ccaattatgc ctgctattgc tatccgtgaa gtggtggaag
    35941 aagcctacgc cgctgacccg gaaatgatcg cctctgcggc ctgtgatatt caggcggtgc
    36001 gtacccgcga cccggcagtc gataaatact caaccccgtt gttatacctg aagggttttc
    36061 atgccttgca ggcctatcgc atcggtcact ggttgtggaa tcaggggcgt cgcgcattgg
    36121 caatctttct gcaaaaccag gtttctgtga cgttccaggt cgatattcac ccggcagcaa
    36181 aaattggccg tggcattatg ctcgaccacg ccacgggcat cgttgtgggt gaaacggcgg
    36241 taattgaaaa cgacgtatcg attctgcaat ctgtgacgct tggcggtacg ggtaaatctg
    36301 gtggtgaccg tcacccgaaa attcgtgaag gtgtgatgat tggcgcgggc gcgaaaatcc
    36361 tcggcaatat tgaagttggt cgcggcgcga agattggcgc aggttccgtg gtgctgcaac
    36421 cggtgccgcc gcatacaacc gccgctggcg ttccggctcg tattgtcggt aaaccagaca
    36481 gcgataagcc atcaatggat atggaccagc atttcaacgg tattaaccat acatttgagt
    36541 atggggatgg gatctaatgt cctgtgatcg tgccggatgc gatgtaatca tctatccggc
    36601 atacagtaac taatctctca acaacgctcc cggatatccc agctgccgcc aggcttcata
    36661 caccactacc gacaccgcat tggacagatt catgctgcgg ctgtccggca ccatcggaat
    36721 gcgaattttt tgttcagcgg gcagggcatc aagaatgctc gctggcaggc cgcgtgtttc
    36781 cgggccgaac atcagataat cgccatcctg atagcttacg gcgctgtgag caggcgtacc
    36841 tttcgtggtg agggcaaaca ggcgctgggg attttctgct tcgaggaacg cgcgatagtc
    36901 atgatgacgc gtaacggcgg taaactcgtg atagtccagc cccgcgcggc gcaggcgctt
    36961 atcgtcccag gcaaatccca tcggttcgat gatatgcaga cgaaagccgg tattagcgca
    37021 aagacggatg atgttgccag tatttggcgg aatttctggt tcgtaaagta cgatgtttag
    37081 catactgccc ccttagtgcg ggggcaggat agcagaaaat tggtgaaccg tgcggccttt
    37141 tcccctcacc ccggccctcc ccctgtctct tgatcaggag atctgatcaa gagacagctc
    37201 cctgagggag agggtt
//
LOCUS       DAJTYD010000018        37173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTYD010000018 DAJTYD010000000
VERSION     DAJTYD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.018"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1547
                     /locus_tag="OO585_000842"
     CDS             <1..1547
                     /locus_tag="OO585_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCR6628518.1"
                     /translation="NRLYLNYLHVTQHNTPDGGIPTIGLPGYSAPSAGTAALNHSGKV
                     DTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVKQDYLMTAIMGGASN
                     ITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGHDVSTGVEFTRETQT
                     NYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYAFDTLQITRDFELNG
                     GIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAKSGNLVNWKAGALYH
                     LTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKANTSEIGTKWQVLDK
                     RLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNITPAWQVIGGYTQQK
                     ATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGARYIGSMHKGSDGAVG
                     TPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKSGYRYHPGKPRTFLL
                     TANMHF"
     gene            1589..2266
                     /gene="ybiX"
                     /locus_tag="OO585_000843"
     CDS             1589..2266
                     /gene="ybiX"
                     /locus_tag="OO585_000843"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6628519.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2340..2606
                     /gene="ybiI"
                     /locus_tag="OO585_000844"
     CDS             2340..2606
                     /gene="ybiI"
                     /locus_tag="OO585_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6628520.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            2871..3131
                     /locus_tag="OO585_000845"
     CDS             2871..3131
                     /locus_tag="OO585_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6628521.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3359..4444)
                     /gene="hcxB"
                     /locus_tag="OO585_000846"
     CDS             complement(3359..4444)
                     /gene="hcxB"
                     /locus_tag="OO585_000846"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6628522.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGYLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(4585..5547)
                     /gene="ybiB"
                     /locus_tag="OO585_000847"
     CDS             complement(4585..5547)
                     /gene="ybiB"
                     /locus_tag="OO585_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6628523.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(5575..7725)
                     /gene="dinG"
                     /locus_tag="OO585_000848"
     CDS             complement(5575..7725)
                     /gene="dinG"
                     /locus_tag="OO585_000848"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6628524.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            7845..8327
                     /gene="ybiA"
                     /locus_tag="OO585_000849"
     CDS             7845..8327
                     /gene="ybiA"
                     /locus_tag="OO585_000849"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6628525.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8559..9923)
                     /gene="rhlE"
                     /locus_tag="OO585_000850"
     CDS             complement(8559..9923)
                     /gene="rhlE"
                     /locus_tag="OO585_000850"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6628526.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10152..10823
                     /gene="cecR"
                     /locus_tag="OO585_000851"
     CDS             10152..10823
                     /gene="cecR"
                     /locus_tag="OO585_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6628527.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10826..11821
                     /gene="hlyD"
                     /locus_tag="OO585_000852"
     CDS             10826..11821
                     /gene="hlyD"
                     /locus_tag="OO585_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6628528.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            11814..13550
                     /locus_tag="OO585_000853"
     CDS             11814..13550
                     /locus_tag="OO585_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6628529.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13543..14676
                     /locus_tag="OO585_000854"
     CDS             13543..14676
                     /locus_tag="OO585_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6628530.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            14687..15793
                     /locus_tag="OO585_000855"
     CDS             14687..15793
                     /locus_tag="OO585_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6628531.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(15755..16165)
                     /locus_tag="OO585_000856"
     CDS             complement(15755..16165)
                     /locus_tag="OO585_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6628532.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16298..17049
                     /locus_tag="OO585_000857"
                     /pseudo
     CDS             16298..17049
                     /locus_tag="OO585_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17046..18287
                     /gene="clsB"
                     /locus_tag="OO585_000858"
     CDS             17046..18287
                     /gene="clsB"
                     /locus_tag="OO585_000858"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6628533.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18287..19243
                     /locus_tag="OO585_000859"
                     /pseudo
     CDS             18287..19243
                     /locus_tag="OO585_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19279..19992)
                     /locus_tag="OO585_000860"
     CDS             complement(19279..19992)
                     /locus_tag="OO585_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6628534.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20198..20902)
                     /locus_tag="OO585_000861"
     CDS             complement(20198..20902)
                     /locus_tag="OO585_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6628535.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21039..21491)
                     /gene="moaE"
                     /locus_tag="OO585_000862"
     CDS             complement(21039..21491)
                     /gene="moaE"
                     /locus_tag="OO585_000862"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6628536.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21493..21738)
                     /gene="moaD"
                     /locus_tag="OO585_000863"
     CDS             complement(21493..21738)
                     /gene="moaD"
                     /locus_tag="OO585_000863"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6628537.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(21731..22216)
                     /gene="moaC"
                     /locus_tag="OO585_000864"
     CDS             complement(21731..22216)
                     /gene="moaC"
                     /locus_tag="OO585_000864"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6628538.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22219..22731)
                     /gene="moaB"
                     /locus_tag="OO585_000865"
     CDS             complement(22219..22731)
                     /gene="moaB"
                     /locus_tag="OO585_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6628539.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(22753..23742)
                     /gene="moaA"
                     /locus_tag="OO585_000866"
     CDS             complement(22753..23742)
                     /gene="moaA"
                     /locus_tag="OO585_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6628540.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(23730..23874)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24139..25047
                     /gene="yvcK"
                     /locus_tag="OO585_000867"
     CDS             24139..25047
                     /gene="yvcK"
                     /locus_tag="OO585_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6628541.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25239..27260)
                     /gene="uvrB"
                     /locus_tag="OO585_000868"
     CDS             complement(25239..27260)
                     /gene="uvrB"
                     /locus_tag="OO585_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6628542.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(27839..28516)
                     /gene="bioD"
                     /locus_tag="OO585_000869"
     CDS             complement(27839..28516)
                     /gene="bioD"
                     /locus_tag="OO585_000869"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6628543.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28509..29264)
                     /gene="bioC"
                     /locus_tag="OO585_000870"
     CDS             complement(28509..29264)
                     /gene="bioC"
                     /locus_tag="OO585_000870"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6628544.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29251..30405)
                     /gene="bioF"
                     /locus_tag="OO585_000871"
     CDS             complement(29251..30405)
                     /gene="bioF"
                     /locus_tag="OO585_000871"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6628545.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30402..31442)
                     /gene="bioB"
                     /locus_tag="OO585_000872"
     CDS             complement(30402..31442)
                     /gene="bioB"
                     /locus_tag="OO585_000872"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6628546.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31529..32818
                     /gene="bioA"
                     /locus_tag="OO585_000873"
     CDS             31529..32818
                     /gene="bioA"
                     /locus_tag="OO585_000873"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6628547.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            32877..33353
                     /locus_tag="OO585_000874"
     CDS             32877..33353
                     /locus_tag="OO585_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6628548.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33505..34788
                     /locus_tag="OO585_000875"
     CDS             33505..34788
                     /locus_tag="OO585_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6628549.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(34929..37166)
                     /locus_tag="OO585_000876"
     CDS             complement(34929..37166)
                     /locus_tag="OO585_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6628550.1"
                     /translation="MFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMD
                     KLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVF
                     GLSAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGE
                     LVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVG
                     PGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYY
                     DGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLL
                     DKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDL
                     AKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQM
                     SAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQ
                     PLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEF
                     TLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMV
                     YAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEV
                     GDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFN
                     KNRQM"
BASE COUNT         8713 a        10060 c         9614 g         8786 t
ORIGIN      
        1 cgaatcgttt gtatcttaat tatctgcatg tcacccagca caacacgcca gacggcggca
       61 ttccgaccat cggtttgccg ggctattctg ccccatctgc gggaacggcg gccctgaatc
      121 attccggaaa agttgatact cataactttt acggcacgga ttccgattac gacgattcga
      181 ccaccgacac cgccaccatg cgttttgagc acgacatcaa cgataacacc accattcgca
      241 atactacccg ttggtcgcgc gtaaagcagg attacctgat gacggcgatt atgggcgggg
      301 cgtcgaatat tactcagccc accagcgatg tgaatagctg gacctggtca cgcacggcga
      361 ataccaaaga tgtgagtaat aaaattctca ccaaccagac caacctgacc tcgacgttct
      421 ataccggttc tatcggtcat gatgtcagta ccggcgtgga atttacccgt gaaacgcaga
      481 ctaactacgg cgttaatccg gtgacgttac ccgcggtaaa tatttatcat cctgacagca
      541 gcattcatcc cggcggcctg acgcgcaacg gcgcaaacgc caatggtcag acggatacct
      601 tcgcaattta cgcctttgat acgctgcaaa tcacccgtga ttttgagctg aacggcggga
      661 tccgtctgga taattatcat actgaatatg acagtgccac cgcctgcggc ggcagcggac
      721 gcggtgccat cacctgccca gctggtgtgg caaaaggttc tccggtcacc accgtcgaca
      781 ccgccaagtc gggcaatctg gtgaactgga aagccggggc gctgtatcac ctgacggaaa
      841 acggtaatgt ctatattaac tatgccgttt cccagcagcc tccgggcggc aacaacttcg
      901 cccttgcgca gtctggcagc ggtaacagtg ccaaccgcac cgattttaaa ccgcaaaaag
      961 ccaacaccag cgagattggc accaaatggc aggttctgga taaacgcctg ttgctcaccg
     1021 ccgcactgtt ccgtactgat atcgaaaatg aagttgagca aaatgatgac ggaacttact
     1081 cgcaatacgg taagaaacgt gtcgaaggct atgagatatc cgtggccggg aatatcactc
     1141 ccgcgtggca ggtgattggc ggctataccc agcaaaaagc aaccatcaaa aacggcaaag
     1201 atgttgccca ggatggttcc tcatcgctgc cgtatacccc ggagcacgcc ttcaccttat
     1261 ggagccagta tcaggcaacc gatgatatct ctgttggcgc gggcgcacgc tatatcggca
     1321 gtatgcataa aggttcagac ggcgcggtgg gaacgccagc gtttaccgaa ggttactggg
     1381 tcgccgatgc caaactgggg tatcgggtta atcgcaatct cgacttccag ctaaacgttt
     1441 acaacctgtt tgataccgat tacgtcgcct caatcaacaa gagcggctac cgttatcacc
     1501 cgggcaagcc aagaaccttc ttgctcacag ccaatatgca tttctgattc agatgtgggg
     1561 cgcaggcccc actttttgga gaaattgtat gatgtaccac attcccggcg tgttatcgcc
     1621 acaggacgtc gctcgttttc gcgaacaact ggaacaagcc gaatgggtgg atggacgcgt
     1681 caccaccggc gcacaaggtg cgcaagttaa gaacaatcaa caggtagaca cccgcagcac
     1741 gttatacgcc gccctgcaaa atgaggtgct gaacgcggtt aaccaacatg ctttattctt
     1801 tgccgcggcc ttgccgcgta ccctttccac gccgctgttt aatcgctatc agaacaatga
     1861 aacctatggt tttcatgtgg atggcgcagt acgcagccat ccacaaaacg gctggatgcg
     1921 cactgacctt tctgccacgc tgtttttaag cgatccacaa agctacgacg gcggcgaact
     1981 ggtcgttaat gacaccttcg gacaacatcg ggtaaaactc ccggcaggtg atctcgtgtt
     2041 gtatccctcc agcagcctgc attgcgtgac acccgtaacc cgcggcgtac gagtggcatc
     2101 atttatgtgg atccagtcga tgatccgcga tgataaaaag cgcgccatgc tgtttgaact
     2161 agacaaaaat attcagtcgc tgaaaagccg ctatggtgaa aatgaagaga tcctgtcgct
     2221 gctcaatctt tatcataatc tgctgcggga atggtcggag atctgacgct tgaactgccc
     2281 aggttcgtcc ctatattagc tacacttaac tgtacaagca ttgatatggg gaggacgata
     2341 tggcatccgg ttgggctaac gatgacgccg tcaacgaaca gatcaacagt acaattgaag
     2401 atgcgattgc ccgcgctcgg ggtgaaattc cgcgtggcga aagcctgtat gaatgtgaag
     2461 agtgcggtgc ccccatcccg caagcccgtc gggaagccat tcctggcgtg cgcttatgta
     2521 ttcattgtca gcaggagaaa gatttacaaa aaccagcgta tacaggatat aatcgcagag
     2581 gttcgaaaga cagccagtta cgttaactac aactggcgag atgcatagcg agtataattt
     2641 gtattttgcg tggcattccg tgcctttaac gctgctgtga ctgccacgcg gcataaaaca
     2701 agtccataag tacaacaaat aaatggttta tcagtatgtt agatgtcaat caatttattt
     2761 gaacaaggcg gtcaattctc ttcgatttta tctctcgaaa aaaaacgtga tactcatcac
     2821 atcgacgaaa caacgtcact tatacaaaaa tcacctgcga gagattaatt atgaaaacta
     2881 tcaatactgt tgttgctgct atggctcttt caactctatc atttggcgtg ttcgcggcgg
     2941 aaccggtaac ggcatcccag gcacagaaca tgaataaaat cggcgtggtt tctgccgatg
     3001 gcgcatccac cctcgatgcc ctggaagcga aactgactga gaaagccgcg gcagccggtg
     3061 ccagtggata cagcatcact tctgccacca acaacaataa attaagcggt actgcggtaa
     3121 tttataagta attcgtagga cggataaggc gtttacgccg catccggcaa cagatgcctg
     3181 atgcgacgct accgcgtctt atcaggccta caaaatccga accgttggac ggatcaggcg
     3241 gttacgctac gtccagcaca agtatttccc tgtctgtttg ccgacagacg catatgctct
     3301 aaccctcatt gatcctatat tacccttgtt tgcccgtccg ttactagacg ggcttttttt
     3361 agctggctaa ctgctgacag aaagcctgca acgtctcttc cgacatacca atctgccgcg
     3421 ccgcatcaca aatggcctgc cagcttcccg catccaatgg aatcccctgc tcctgccgtt
     3481 cgcgacgcgt gttcacttcc cactcgcccg gtagcaaaat cggcttatcg tcatcatgcg
     3541 gcgaggcttt cacccactcg gcaaaggctt cagtctgcgc gctacaatcc ggcgcgccga
     3601 acagttccgg gttgatgatg atagtggtca tgcagttaag aatggcatcg ggactggttt
     3661 gtaacgtttc ctgatgcgtc gttttaccgc cagaaagcgc cccgccaaga atttcacaca
     3721 tcgctgcaag ggcgtagcct ttatgttcgg caaaggtcaa cagcgacccc aacggcgact
     3781 cctgcattac cgccggattg gtcgtcggca cgccgttaac gtcaatcagg caacctggcg
     3841 gcacggggac gcctttatgc caggcgacgc gagttttacc aaatgcgatg gcgctggtgg
     3901 cgtaatcaag caacagcggg aaattatctt tacgagggaa aaccacacag aacggattgg
     3961 tgccaaagcg gctgtcgcga ccgtggaacg gtgcgaccat tggaataccg accacgctaa
     4021 caaagtggat agagacaaac cccgccgctg cacactgctc cgcccagtaa ccgatacggc
     4081 cgatatgatg cgagttatgt agcgccacgg cagcaatacc gtgctgatgc gctttctcaa
     4141 tccccagcgc catcgcttcg tgtgccgcga cctgaccgaa tgcgcgatcg ccatcgagcg
     4201 tgactgccgc ccccgcctct ttaacgattt tggcatgatg gttaatttgc agatacccct
     4261 gactccagga acgcacatag cttgggatca tgccaatacc atgagaatca tgccctgcca
     4321 ggtttgccgc gattaaatga tcggccacta atttcgcttc ttgttcctcg ctacccatct
     4381 gacgaaatac agcctgaata aaactgtgca gcgtctgagc atcaaagcga tgaccacttt
     4441 ccatggttaa ctcctgttta tgttatgtgt ttgttgtttt tttatgctgc acgccgggcg
     4501 cgggaaggtc aataaagatg caggaatttc atattgcggt ggggtaaaag ccggatgaca
     4561 tggctcatcc ggctgagaaa agaattaaaa tgcctgatta acgcgcgcca ggccgtcgct
     4621 gatagttgcc gcttcacccg tagcctccag gcagcaagcc atctggattt tcagcgattc
     4681 gggaatcggt tcgctgccag caaggcaacg ctcaatccac tgcgccgtgg tttctgggtc
     4741 ttttgcttgt ggcagtaact cgctaccagc agtgtcctgt ttttcataca gcacccgcat
     4801 cccttcacgg tcaatgagat tgatctgcgg gcagcgctgc ggattagcat acacttcgcc
     4861 ttcagtacca tgcattagta gcgcccgccc gccgatatcg ctaaagaact tcgcgactcg
     4921 cccgatgtat tccggatgcg aaacgctgga aagacgcagc gcctcacctt cggcaaatgg
     4981 cgtcgccagt ttcgccaggg tatgtgcact gttacgcacg cccatccgcc agcgcatcgc
     5041 cagttgtttt tccagcggcg ggcaaaacgc gccgacaggc ataaacaccg gttgatgttc
     5101 gtcgagcttc gcctgcgcct gcccgccgtg caacgttggt gtaataccca tcagttcgaa
     5161 aatggtttct gtcagcacgc gagttggatc ttcgctaacc ccgtgaacca ccacaggaaa
     5221 accgagtttg tgcaggagaa tagccaacaa cggcgtcagg ttggcctgtt tacgcgcgcc
     5281 gttgtaactg gggatgacaa tcggcatcgg cttgcctgca ggcggcgtca gcttgatggt
     5341 gtgattttgc atggcttcgt aaaagccgag catctctgct tccccttccc ctttgatacg
     5401 tagcgcaatc aatacgccgc ccaactcaag gtcagggact tcaccattga gcatatgggc
     5461 atacagaccg cgcgcagtat cccggtctaa atcgcgcgcg tggtttttcc cgcgcccgat
     5521 ctctttaatg attttgcgat agtccattta cgactcctta cctgactcac atcattaacg
     5581 ccgccgacgg cgtggggatt tcgttttttc tttcttttta actatacctt cagggacttc
     5641 cggttgctct atcggaaata ccggtaatgc atccagtagt cgcttgccat aatttttggt
     5701 cagcaagcgt ttgtcgtaga taaccacttc gccccagcaa ccgtggcttc gaatcagtcg
     5761 cccaacctgc tgaatcaggt taaacgaggc gctcggcagg ctttgcacct caaacggata
     5821 gcggttgagg cttttcagcc attccccttc ggtgatcacc accgggctgt cgatgggcgg
     5881 aaaagcgatt ttgtggatat gcacctggct gagcagatca cctttcaaat caagcccttc
     5941 ggcaaatgac tgtaagccca ccagcacact acgctcaccg ttggcaacgc gtttgcggtg
     6001 cagttcaact aagcgataac gcggctgatc gccctgcacc agcagcatca ggcgtaaatc
     6061 cgtcacatag tcgagaaagc gctgcatcgc ccgtccgctg gcaaacagta ccaacatacc
     6121 gagatgtttt ttgctctcca cctgctcacg gaaaaaggcc gccatttcgg caatatgctg
     6181 ctcttcgttg tcgatggaag gctcaaagcg catccgggga ataacaattt tgccctgttc
     6241 gcagtggtta aagggggaat ccagcgccac aaaacggtcg cccgctttct ctttcagccc
     6301 gctcatctcc tgcaaacgcg aaaaactgtt cagcgaacgc aaggttgcgg aggtgacaat
     6361 aatgtgcgga atactgcgcc acagcagcct ttccagctga tcgctgacac gtattcccac
     6421 gcagtgaaac cagagatgca gctgcccttc gcgctcttcc cgcgtcgccc atttggtcac
     6481 cggcgcaccg gaagattgcg ccagagacgc cagccgccag agtttgcttt gcgcctcgaa
     6541 catccccaac gcgcggttca tctgcaaaat caaccgatgc agacgcacaa tatcgtggct
     6601 gccggttttc tcacttaaat cgttaagaaa taactccgcc agaccacgca gcatctcggt
     6661 gagttttgcc agccgctggc agatctccag cacttcatca ggcagttcgc ccatcgcaaa
     6721 acggtgctct gcctcctgcc cggcaggcat gtagagattg agaatgttgt ttaacgaggc
     6781 gataagctca tacaactctt cacaatgcgc attcaaacgt tcagggatcg ccagcggtgg
     6841 gatggtcttc gggcgaaact gctccatgca ggtagcgacc agtttcgtga acaagtccag
     6901 ctgtagtcga taccagggcg cggtgatttc agcgctcatc tccagcgcat cccgggccac
     6961 atctggcagg tgatgacctt cgtccagcac cagcagcaga tttttcgggt caggcagtac
     7021 ggcttcgctt tccatcgccg ccatcaccag cgcatggttt gccaccacca cttccgcttc
     7081 ctgaatctcc cgacgagcaa caaaaaacgg gcattcacga tagtagtaac agttacggtt
     7141 aaggcagctg gctttgtcgg tactcaaacg acgccagaga tcatcatcta tagcgatatc
     7201 cgtatgatcg cgcagaccat cccatttata agtgtcgaga tcgcccttca gcttcgcaca
     7261 acgtttttgc tcttcctgat tatttggcgt cagttcgtcg tcaagaaacg ccagcagatc
     7321 ctgttgcgtg ggttcagtgc tggcgagcgc cgtcagatta cgcggacaaa cgtagcgccc
     7381 acgcccgaaa gcggcagtga atttaagatc gggaatgatc tttttcagca gcggtaaatc
     7441 tttgctgtaa atctgatcct gcaacgccac gttggcggta ctcaccacca gcgttttttg
     7501 ctcttcgcgg gcaatggcga tgccggggat caaataggag agcgttttcc caacgccggt
     7561 gggggcttca atcgccagat gccgcccttc ttctccggcc agcgttttgg cgacgtccgc
     7621 aatcatctgc cgctgcggcg cacggggaat aaagtcgggg atctgttcct gaagcgcctt
     7681 ataccaggcg gcaatttgcg ctttaagcgc ggcggttaat gccatgagaa aacctgaaat
     7741 actgtataaa cagccaatat tgtggcattt ttcggttttc aggggtaatg gtttttctgt
     7801 ttctggaaag cggctcgcaa taaccaccaa cataatgcgg tttaatgccc gttcgagcac
     7861 aaaggatcca acacgtcatg caagacacca tcataaattt ctacagcacc agcgatgact
     7921 acggtgattt ttctaacttt gccgcctggc ccatcaaggt tgacgggaaa acctggccca
     7981 cctccgaaca ctattttcag gcacaaaaat tccttgatga aaaataccgc gaagagatcc
     8041 gtcgggtttc ttcccccatg gtcgccgctc gcatggggcg taatcgttcc aagccacttc
     8101 gtaaaaactg ggagtcggtt aaagaacagg tgatgcgtaa agcacttcgt gctaaattcg
     8161 aacagcatgc agaactgcgt gcgctcttgc tggcaaccgc gcccgcaaaa ctggttgagc
     8221 atacggaaaa cgatgcttac tggggagacg gtggtaatgg taagggcaag aatcgactgg
     8281 gctacctttt aatggagttg cgcgaacaat tggctataga gaagtaacgt ctcggtggct
     8341 tatgggcggt aaaacagata aattttttcc cgtgttctca aattgctgac aagtgcttgt
     8401 tgtgaaatgc cggatgcggc gtaaacgcct tatccggcct acaaaagcat gcaaattcaa
     8461 tatattgcag agttaacgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgt
     8521 tcatcagcct gatgccgggc atagcccggc ataaaagatt actgcgcagc ggcaggttta
     8581 cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg catcgccgag gcggcgagac
     8641 ggtttttctg caggtttcgc gcttgcagat tttgcgccac cttccccacg gcgtggttgc
     8701 tgttgaccgc gaccaccacc ttgcccacga ccgccgccgc cacgttgctg gcgaccgttc
     8761 tggatcggtt cggctttgat tgacgggtcc ggctcatagc ccggaatcgc aatgcgcggg
     8821 atctcttttt tcagcagttt ttcgatatca cgcagcagtt tgtgttcatc aacacacacc
     8881 agcgacaacg cttcaccggt agcagccgca cgaccggtac gcccgatacg gtggacataa
     8941 tcttccggta cgtttggcag ttcatagttg acgacgtgcg gcaactcttc gatatccaga
     9001 ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt cactcgattt aaaatcagcc
     9061 agcgcacggg tacgcgcacc ttgcgattta ttcccgtgga tcgccgcact acggatgcca
     9121 tctttattga gctgctcagc cagatggtta gcgccgtgtt tggtacgggt aaacaccagc
     9181 acctgctgcc agttcccttt accaatcatg tgcgacagca attcgcgttt gcgtttctta
     9241 tcgacaaagt gaacgtgctg ggtcacctga tcagacgcgg tattgcggcg tgccacttcg
     9301 atttccagcg ggttgtgcaa cagtttttcc gccagggctt taatatcgtc agagaaggtc
     9361 gcagagaaca gcaggttctg gcgtttcgct ggcagttttg ttaacacgcg acggatatcg
     9421 tggataaagc ccatgtcgag catgcggtca gcttcatcga ggacgaggat ttcaacctga
     9481 tccagcttca ctgcattttg atgttccagg tccagcaaac gtcccggtgt tgccaccaga
     9541 acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa tgctgacacc gccaaacacc
     9601 accagcgaac gaatgttcaa atatttgctg taatcacgga cgttttcgcc aatctgcgca
     9661 gccagttcac gggtcggggt aagaatgagc gcacgtaccg gacgacgccc tttggcgtgc
     9721 ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt tttaccggta
     9781 ccggtctggg cgctagccat caggtcgcgg ccttccagca ccgcagggat cgcctgctgc
     9841 tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat atcagggctt
     9901 aaacccaaag aatcgaaaga cataactact ccgaaccgcc ccgaccgtta ccggtgtagt
     9961 tttcagggag atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag ggagcggagt
    10021 gtagcagttt ttgtgacgca gcgcataaat tatccctgtg actggcgttg ctaaaattct
    10081 tagccatact ggacaactcc cctatggagt cataatctta atcaatcatt tgattaagaa
    10141 tggactgtgc gatgaataat cctgccatga caatcaaggg tgaacaggcg aaaaaacagc
    10201 tgattgctgc cgcactggcg cagtttggtg aatatggaat gaacaccacc actcgcgaga
    10261 tagccgccca ggccgggcag aatatcgctg ccatcaccta ctacttcggt tcgaaagaag
    10321 atttgtacct cgcctgcgcc cagtggattg ccgattttat tggcgagcag ttccgtccgc
    10381 atgccgagga agccgaacgc ttgttcgcgc aaccacagcc tgatcgggct gccatccgtg
    10441 aactgatcct tcgcgcctgc aggaacatga ttaagctgct cacccaggat gacaccgtca
    10501 acctcagcaa gtttatctcc cgtgagcagc tctctcccac ggcagcctac cacctggtgc
    10561 acgaacaggt gattagtccg ctacacagcc acctgacacg cctgattgcc gcctggaccg
    10621 gctgcgacgc caatgacacc cgcatgatcc ttcataccca tgcgttgatt ggtgagattc
    10681 tggcgttccg tcttggcaaa gaaacaattc tgttacgtac cggctggacc gcgttcgatg
    10741 aagaaaagac cgaactgatc aaccagacgg tgacctgtca tatcgacctg attttgcaag
    10801 gattatcgca aaggagtttg tagtgatgaa aaaacctgtc gtgatcggac tggcggtagt
    10861 ggtacttgcc gccgtggttg ccggaggcta ctggtggtat caaagccgcc aggataacgg
    10921 cctgaagctg tatggcaacg tggatattcg tacggtgaat cttagtttcc gtgttggggg
    10981 gcgcgttgaa tcgctggcgg tggacgaagg tgatgcgatc aaagcgggcc aggtgctggg
    11041 cgaactggat cacaagccgt atgagattgc cctgatgcag gtgaaagcgg gcgtctcggt
    11101 ggcacaggcg cagtatgacc tgatgcttgc cgggtatcgc gatgaagaaa tcgctcaggc
    11161 cgccgcagcg gtgaaacagg cgcaagccgc ctatgactat gcgcagaact tctataaccg
    11221 ccagcaagga ctgtggaaaa gccgcaccat ttcggcaaat gacctggaaa atgcccgctc
    11281 ctcgcgcgac caggcacagg caacgctgaa atcagcacag gataaattgc gtcagtaccg
    11341 ttccggtaac cgtgaacagg acatcgctca ggcgaaagcc agcctcgaac aggcgcaggc
    11401 gcaactggcg caggcggagc tgaatttaca ggactcaacg ttgatagcac cgtctgatgg
    11461 cacactgtta acgcgcgcgg tggagccagg cacggttctc aatgaaggtg gcacggtgtt
    11521 taccgtttca ctaacgcgtc cggtgtgggt gcgcgcttat gttgatgaac ggaatcttga
    11581 ccaggcccag ccggggcgca aagtgctgct ttataccgat ggtcgcccgg acaagccgta
    11641 tcacgggcag attggtttcg tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac
    11701 accggatctg cgtaccgacc tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga
    11761 tgcgttacgc cagggaatgc cggtgacggt gcaattcggt aacgaggcag gacatgaatg
    11821 atgccgttat cacgctgaac ggcctggaaa aacgctttcc gggcatgggc aagcccgccg
    11881 tcgcgccgct cgattgtacc attcacgccg gttatgtgac ggggctggtg gggccggatg
    11941 gtgcaggtaa aaccacgcta atgcggatgc tggcgggatt actgaaaccc gacagcggca
    12001 atgccacgat gattggcttt gatccaatca aaaacgacgg cgcgctgcac gccgtgctcg
    12061 gctatatgcc gcagaaattt ggtctgtatg aagatctcac ggtgatggag aacctcaatc
    12121 tgtacgcgga tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc
    12181 tggagtttac gtctcttggg ccgtttaccg gacgcctggc gggcaagctc tccggtggga
    12241 tgaaacaaaa actcggtctg gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg
    12301 atgaacccgg cgtcggcgtt gaccctatct cacggcgcga actgtggcag atggtgcatg
    12361 agctggcaag cgaagggatg ttaatcctct ggagtacctc gtatctcgac gaagccgagc
    12421 agtgccgtga cgtgttactg atgaatgaag gcgagttgct gtatcagggg gaaccaaaag
    12481 ccctgacaca aaccatggcc ggacgcagct ttctgatgac cagtccacac gagggcaacc
    12541 gcaaactgtt gcaacgcgcc ttgaaactgc cacaggtcag cgacggcatg attcagggga
    12601 aatcggtacg tctgatcctc aaaaaagagg ccacaccaga cgatattcgc catgccgacg
    12661 ggatgccgga aatcaacatc aacgaaacta cgccgcgttt tgaagatgca tttattgatt
    12721 tgctgggtgg tgccggaacc tcagaatcgc cgctgggtgc aatattgcat acggtagaag
    12781 gcacacccgg cgagacggtg atcgaagcga aagaactgac caagaaattt ggtgattttg
    12841 ccgccaccga tcacgtcaac tttgccgtta aacgtgggga gatttttggt ttgctggggc
    12901 caaacggcgc gggtaaatcg accaccttta agatgatgtg cggtttgctg gtgccgactt
    12961 ccggccaggc gctggtgctg gggatggatc tgaaagagag ttccggtaaa gcgcgccagc
    13021 atctcggcta tatggcgcaa aaattttcgc tctacggcaa cctgacggtc gaacagaatt
    13081 tacgcttttt ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa aaaatctccc
    13141 gtatgagcga ggcatttggc ctgaaaagta tcgcctccca cgcgaccgat gaactgccat
    13201 taggttttaa acagcggctg gcgctggcct gttcgctgat gcatgaaccg gacattctgt
    13261 ttctcgacga accaacgtcc ggcgttgatc ccctcacccg ccgcgaattt tggctgcata
    13321 tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac cactcacttt atggatgaag
    13381 cagaatattg cgaccgcatc ggcctggtgt accgtgggaa attaattgcc agcggcacgc
    13441 cggatgattt gaaagcgcag tcggccaacg acgagcaacc cgatcccacc atggagcaag
    13501 cctttattca gttgatccac gactgggata aggagcatag caatgagtaa cccgatcctg
    13561 tcctggcgtc gcgtacgggc gctgtgcgtt aaagagacgc ggcagatcgt tcgcgatccg
    13621 agtagctggc tgattgcggt agtgatcccg ctgctactgc tgtttatttt tggttacggc
    13681 attaacctcg actccagcaa gctgcgggtc gggattttac tggaacagcg tagcgaagcg
    13741 gcgctggatt tcacccacac catgaccggt tcgccctaca tcgacgccac catcagcgat
    13801 aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt
    13861 ccggtggatt ttgcggaaca gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc
    13921 accgacggca gtgagccgaa taccgctaac tttgtacagg ggtatgtcga agggatctgg
    13981 cagatctggc aaatgcagcg agcggaggac aacgggcaga cttttgaacc gcttattgat
    14041 gtacaaaccc gctactggtt taacccggcg gcgattagcc agcacttcat tatccccggt
    14101 gcggtgacca ttatcatgac ggtcatcggc gcgattctca cctcgctggt ggtggcgcga
    14161 gaatgggaac gcggcaccat ggaggctctg ctctctacgg agattacccg cacggaactg
    14221 ctgctgtgta agctgatccc ttattacttt ctcgggatgc tggcgatgtt gctgtgtatg
    14281 ctggtgtcag tgtttattct cggcgtgccg tatcgcgggt cgctgctgat tctgtttttt
    14341 atctccagcc tgtttttact cagtaccctg gggatggggc tgctgatttc cacgattacc
    14401 cgcaaccagt tcaatgccgc tcaggtcgcc ctgaacgccg cttttctgcc gtcgattatg
    14461 ctttccggct ttatttttca gatcgacagt atgcccgcgg tgatccgcgc ggtgacgtac
    14521 attattcccg ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt
    14581 ccagtggtgc tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc
    14641 ctgacgtggc tgaaaaccaa acgtcggctg gattagggag aagagcatgt ttcatcgctt
    14701 atggacgtta atccgcaaag agttgcagtc gctgctgcgc gaaccgcaaa cccgcgcgat
    14761 tctgatttta cctgtgctta ttcaggtgat tctgttcccg ttcgccgcca cgctggaagt
    14821 gactaacgcc accatcgcca tctacgatga agataacggc gagcattcgg tggagctgac
    14881 ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg ctgctgaaaa gcccacagga
    14941 gatccgccca accatcgaca cacaaaaggc gttactcctg gtgcgtttcc cggctgactt
    15001 ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag ttgatcctcg acgggcgtaa
    15061 ctccaacagt gcgcaaattg ccaccaacta cctgcaacag atcgtcaaaa attatcagca
    15121 ggaactgctg gaaggaaaac cgaaacctaa caacagcgag ctggtggtac gcaactggta
    15181 taacccgaat ctcgactaca aatggtttgt ggtgccgtca ctgatcgcca tgatcaccac
    15241 tatcggcgta atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct
    15301 cgatcagcta ctggtttcgc cgctcaccac ctggcagatc ttcatcggca aagccgtacc
    15361 ggcgttaatt gtcgccacct tccaggccac cattgtgctg gcgattggta tctgggcgta
    15421 tcaaatcccc ttcgccggat cgctggcgct gttctacttt acgatggtga tttacggttt
    15481 atcgctggtg ggattcggtc tgttgatttc atcactctgc tcgacacaac agcaggcgtt
    15541 tatcggcgtg tttgtcttta tgatgcccgc cattctcctt tccggttacg tttcgccggt
    15601 ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt aaccctattc gccactttac
    15661 ggacattacc aagcagattt atttgaagga tgcgagtctg gatattgtgt ggaatagttt
    15721 gtggccgcta ctggtgataa cggccacgac agggtcagcg gcgtacgcga tgtttagacg
    15781 taaggtgatg taacttctta tctttcgcca gcaaagacac taccgccggg ccggcaagga
    15841 ttgccagccc tgcaattgcc agcaaaaagt tgttttccag tacgcgggac aacagccaca
    15901 gcacaatcag cgctgcgcca aaataccagg tcgtggtcag ctcttccaac acgtctgcca
    15961 cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa catcatcact accgtgacac
    16021 aatacagtgc gcataatccc acgccgacgt gaaccagagt cttactcatc cagcccacca
    16081 ccagcagcgc cactaccagt aaatgcaaca taatctgcca gcaacttaga cccgttgcga
    16141 cacgaacacg ttgttgccac ttcatggctt ctctcctgaa gaatttatct ctacttactc
    16201 agagtaccga actttacgga aaattccgta agaccgcacc tttttttgac gccgactaca
    16261 ctatttcaat gagataagag gcaaagacag gaaaattatg cccgatcaaa cacaacaatt
    16321 ttcgttcaag gtgctcacca tcaacattca aaaaggtttt accgcgttta accgacgctt
    16381 cattttgccg gaacttcgcg acgcggtgcg taccgtcagc gccgatattg tttgcctgca
    16441 ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc ccgatacctc
    16501 gcactacgag tttctcgccg acactatgtg gagcgatttt gcctacggtc gcaatgccgt
    16561 atacccggaa gggcatcacg gcaacgccgt actgtcgcgt tatcccattg aacattatga
    16621 gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc gtgctctact gccgcattgt
    16681 gccgccgatg accggaaaag cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc
    16741 gcaccgtcag gcgcagcttg cgatggggtg aatgagctac cggacggcga accggtattg
    16801 gtggcgggtg atttcaatga ctggcggcaa aaagctaatc atccgttaaa agtgcaggcc
    16861 ggactggatg agatttttac ccgcgcccac ggacgcccgg cgcgcacgtt tccggtgcaa
    16921 tttcctctac tacgactgga caggatctac gtcaaaaatg ccagcgccag cgcgccaacc
    16981 gcgttgccgc tgcggacatg gcgacacctt tctgatcatg cccctttaag tgcggagatt
    17041 catttatgaa atgtagctgg cgcgaaggca ataagatcca gttgctggaa aacggcgagc
    17101 aatattatcc cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc attcttgaaa
    17161 cgtttatctg gtttgaggat aacgtcggca aacagctgca tgcggcacta ctggcagcag
    17221 cgcaacgcgg ggttaaagcg gaagtcttgc tggatggcta cggttcgccg gatctcagcg
    17281 atgagtttgt caataaactg acggcagctg gcgtagtgtt ccgctactac gatccccgcc
    17341 ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa attgtggtga
    17401 tcgacgcgcg tatcgccttt attggcgggc tgaattactc cgccgagcat atgtccagct
    17461 acggtccaga ggctaaacag gattacgcgg tacgccttga agggccgatt gtcgaagata
    17521 ttctccagtt tgagctggaa aacctgcctg gacagagcgc ggcacggcgc tggtggcgac
    17581 gtcatcacaa agcggaagag aaccgccagc cgggagaagc gcaggtattg ctggtctggc
    17641 gcgataacga agaacatcgc gatgatattg aacgccatta tctgaaaatg ctcactcagg
    17701 cgcggcggga agtgattatc gccaacgcct acttcttccc cggctatcga tttttacacg
    17761 ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact gatcattcag ggcgaaccgg
    17821 atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt aaaggcggcg
    17881 ttcaggtttt tgagtaccgc cgccgcccgc tccacggcaa agtggcattg atggacgatc
    17941 actgggcgac agtagggtcc agtaatctcg atccgctcag tttgtcactg aatctcgaag
    18001 caaatgtcat catccacgat cgtcatttta accagacgct gcgcgataat ctgaacggca
    18061 ttattgccgc agattgtcag caggtggatg aaaccatgct gcccaaacgc acctggtgga
    18121 acctgaccaa aagcgtgctg gcgttccact ttttacgcca cttcccggcg ctggttggct
    18181 ggcatccggc acacacacca cgtctgacgc aggttgatcc ccccgcacaa ccgacaatgg
    18241 aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa accctgatga gtaaatcaca
    18301 cccgcgctag cgcttagcaa agaagatcct cacctggctg ttttttatcg cggtgattgt
    18361 gttgctggtg gtctacgcca aaaaagtgga ctgggaagag gtctggaagg tcatccgcga
    18421 ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct atctgattta
    18481 cggctgctat gacctgctcg cccgctttta ctgcggtcac aaactggcga agcgccaggt
    18541 gatgctggtg tcgtttatct gctacgcctt caacctgacg ctcagtacct gggtcggcgg
    18601 cattggtatg cgctatcgtt tgtactcccg actagggtta ccgggcagca ctattacgcg
    18661 gattttctcg ctcagtatta ccaccaactg gctgggctat attttactgg cagggattat
    18721 ctttaccgca ggcgtggtgg tgttgccgga tcactggtat gtcgctcaaa ctacgctgcg
    18781 tattctcggc attggcttac tgatgattat cgcggtttat ttgtggtttt gcgctttcgc
    18841 gaagcaccgc catatgacca tcaaagggca aaagctggtg ctgccttcat ggaaattcgc
    18901 cctcactcag atgctgattt ccagcgttaa ctggatggta atgggggcga ttatctggct
    18961 gttacttggt caaagcgtga actatttctt tgtactgggc gtgttactgg ttagtagtat
    19021 tgctggcgtc atcgtgcata ttccggcggg gatcggtgtg ctggaagcgg tgtttatcgc
    19081 actactggct ggggagcata cctcgaaagg gagcattatc gccgcgctgc tcgcttatcg
    19141 cgtcctgtat tactttatcc cgctgctgct ggcgctgatc tgttacctgc tactggaaag
    19201 ccaggcgaag aagctacggg cgaaaaatga agcggcgatg tgaaatttag ttaagttctt
    19261 tagtatctgc attcacgact aatgaaaaaa acgcgtatgc ctttgccaga caagcgttat
    19321 agctatcgcg gcaagtatcc caaagaagcc gataaaatag taatagagtg tcaatgcacc
    19381 tgtaaaagcg caacgaaccg gactccacaa ctcttcacta tggcattttc ctgccagttc
    19441 aaggagctta tttcgtccat gcgagataat gcctggccat aacacaatca ctaagcaact
    19501 tattacccaa atgggccgtt cactcattaa tgccgcattc acgatgatta ccagggccaa
    19561 ccctgtcaac gtcatcataa tgataaaacg gtgagaacca ggatcaacat taaataataa
    19621 accggccagc atactgatta aaaacatcgc gcccgcagtg ctgaacacaa tgataatggc
    19681 atatacagga actaaacttg caacgacaat cgccaccaaa gagagtaaaa acagatatat
    19741 tacgcctgcg atgacggcat ttatcttgtt gatatcctta cttaagccat acatacaaac
    19801 taataataac cctatggcga catacattgt tggtgaatca tatttggttg ccgtaatttc
    19861 gagatactta gctccccagg ccagtcctgc ggttgccacc aaacctaaag cggtaatgag
    19921 ccatataccg tttaatatcc tggtctcgtg ctgaaaatct aatttattgc tgttttgtga
    19981 gtatgattcc attcttttag ttgtccttag aaaaaatatt aatattcacc atcaataaat
    20041 ttaaacccac gcatacaaaa tacttttgta tttatggata cggaaaacgc ttccgctcgg
    20101 ccataaggcg tattttatac ctggtacttg ttaagtgtcg tttttagtaa gaaaagaaat
    20161 cccgacacat ggccgggata ttgatggtga aaaagaatta acggcggttg ccaaaaattc
    20221 gcagcaacat caggaacagg ttgatgaagt ccagatacaa ggttaacgcg ccaagaatgg
    20281 aatatttgcg caggttcgac gtgtcgcggg tatcaatctg ctcacccata tttttcaatt
    20341 tctgagtgtc atacgccgtc aatccgacaa agacaatcac gccgatgtag gtaactgccc
    20401 acatcaatgc ttcgcttttc agccagaagt tgaccagcga tgccagcaca atgccgatta
    20461 acgccataaa cagcatattg ccaaagccac ttaaatcgcg cttcgtggtg taaccgtaca
    20521 ggctcattgc gccgaacatc ccggcagtaa cgacgaaagt actggcgata gaagcagcgg
    20581 tatagacaat gaatatactg gaaagcgtaa gacccgtcag cgccgaataa agcataaaga
    20641 gcatcgtcgt tacacctgcg ctcagctttt gaatcatcgc tgataacaca ataaccaatg
    20701 ctaattgcgc gatgatcaga ccgattaaaa agacacggtt agtgaacaac agctccatca
    20761 cggccgcgga attagccgca taccaggcaa caaatgcggt cagcaacaag ccaacggtca
    20821 tccagccata gacttgcgcc atataagttt gcaagccagc ccggggttgt acgattgaat
    20881 cagaacgtgg gaatctgtcc atgacgatct cctgaagata taaggaatat cttaaggata
    20941 ctgcaaaatg atgaggctgt gcatcgacgc agcgtaaacg catgtactga gcggtgaaat
    21001 tgccggacgc agcggtgcct tatccggcta acaaaaaact accagcgttt tgccgcctgc
    21061 tgatcgctct cccgagcttc aacccagcgg tcgccttccg gcgtggcttc gcgcttccag
    21121 aacggtgcgc gggttttgag ataatccata ataaactgcc cggcttcaaa cgcactgctg
    21181 cgatgcgcac tggtgacacc ggcaaaaacg atttcatcgc ccggccataa ttccccgatg
    21241 cggtgaatca cagtgacgcg ccccagcggc cagcggttac gcgcttcatc aacaatttct
    21301 gccagtgctt tttcagtcat tcccggatag tgttcgaggg ttaatgcttt gacgctgtcg
    21361 cccaggttat ggttgcgcac cttaccagta aaggtgacta ccgcaccgtc ttcgtcacgc
    21421 tccgccagcc acgggtactc ttctcctacg ctgaacggct gcggaccaac aacaattttg
    21481 gtttctgcca tcttaacctc cggttaccgg cgggaagaaa gctacttcgt cgccgtcagt
    21541 cagcggatgg tcaaaactca ccagcgtctg gttgacggca gccagtaatt tgccatcttc
    21601 cagcgccagc gcccagcgat cgctctgcgc agccatgtgc tggcgtaacg cttcaacagt
    21661 tgggaaatcc gcagccactt cggttgcatc tgttcccacc aactcgcgca cctgcgcgaa
    21721 aaaaagaact ttaatcatcc gcttccacct taaagtcacc cgacttgccg ccgctcttcg
    21781 ccagcaaacg taccggacca atcaccatat ctttttgcac cgctttgcac atgtcataaa
    21841 tggtcagcgc cgccacggag gccgcggtta atgcttccat ttcgacaccg gttttcccgg
    21901 tcaggcggca taaggtttct atacgcaccc gattgtgctc cggctcggcc tgtaaattga
    21961 cttcaacttt gctgagcatc agcggatgac agagcgggat cagatcccag gtgcgttttg
    22021 ccgcctgaat accggcaata cgcgcagtgg caaatacgtc gcctttgtgg tggcgaccat
    22081 caataatcat cgccagcgtc tcgctgcgca tggtgacaaa ggcttccgcc cgcgcttcac
    22141 gcacggtttc cgctttggcg gagacatcca ccatgtgcgc ttcgccagcg gcgttgatat
    22201 gggtcagttg cgacatactt atttcttcaa atgtggatgg aaattacacg gacgcgtacg
    22261 ggcatccagc tgcggcgcga tgatattttc ccatgcggta cggcacgctt tggtcgaacc
    22321 cggcatggcg aaaatcagcg ttttgttggc gacgcccgca accgcacgag attgcaacgt
    22381 ggaagtgcca atctcttcaa acgacaacat ccggaacact tcaccaaaac cttcaacttc
    22441 acggtcgaac aacggcagca atgcttcggg agcctgatca ccttccgtca ggccagtacc
    22501 acccgtaatc aataccactt gtacatcgtc gctggcgatc cacgcagata cctgagcgcg
    22561 aatagcgtag cggttttctt tcacaatggc tttatcgaca acgtgatggc ccgcttcttg
    22621 cgccgaatcg cgcagatagt gaccggaggt atcgtcttct tcaccgcgac gattagaaac
    22681 cgtaagaata gcaatacggg tcgggataaa ttcagtgctt acctgactca tctgatctct
    22741 ccttttgacg ttttagccgc caatgtacga taagttttgc gtaataccgg tgttgttttg
    22801 atgcaggaaa tgggtctgtt tcttctcccg cagcgccgct gaaatacgcg cttccagcgc
    22861 ctgttgctgg gtatcgtctt ccagcagatc gcgcaggtta acgccgcctt caccaaacag
    22921 gcagagatgg agtttaccaa tggaggaaac gcgcaggcgg ttgcaagtgg cgcagaagtc
    22981 tttttcatac ggcatgataa ggccaatctc tccggcgtaa tctggatggc aaaagacttg
    23041 cgcgggaccg tcgctgcgtt gacgtaattg gtggatccag ccgcgacgca gtagctcgtc
    23101 acgcagaacc tgaccagaga tgtgatgctt acggaagagc tcgctgccct cgcccgtttc
    23161 catcagttcg atgaaacgca gctggatagg gcgatgctgg atccagttca gaaaggtgtc
    23221 gagctggtga tgattaacat cacgcatcag cacggtattg actttgacct tctcaaaacc
    23281 ggcctcaaat gcagcatcaa tccctgccat gacctggttg aatttatcct gcccggtaat
    23341 agcgtgaaac tggcgggcgt ccagactgtc gacactgacg ttaatgccag taagtcccgc
    23401 atcgcgccag ttcgccacat cgcgttccag acggtaacca ttggtggtga ccgcaatctg
    23461 gcggatagcg tcgttttccc gcacagcggc gatgatatcg gtaaagtcgc ggcgtaaaga
    23521 cggctcacct cctgtcaggc gcactttttc ggtgcccaga ctggcgaagg cgcgcgtaac
    23581 ccggcgaatt tcatcgacgg taagaaagcc tttattggtg acgccgctcg gtttgtagcc
    23641 atccggcagg cagtaggtgc aacgaaagtt acacacatcg gtaatcgaca ggcgcaagta
    23701 gtaaaactta cgcgcaaatg catcagtcag ttgtgaagcc atgtacacct ttccagatac
    23761 gggaggcgaa gtcatttctt ccttcgccct ggtggcaatc tttacgagca ttgtcacggc
    23821 caaagcaccg tatcagttga cccaggtgca gaggctagag tgtttagtgg ttaagccgat
    23881 actagcgtgt aaatgagatt tttaccatcc acactttcgc tatgcaatca tgtatatagc
    23941 gtcatgatcg tgctatttcg ctacagaata agggaaaatg gcagttttac tttgatatac
    24001 atcatcaggg aggcatagcg tgcatcgtcc tttagaggta gaagaaaata gtttgtatcc
    24061 tcaatattgg caggttaatt gctgtttccc cgcaatttgc gctactgtag cgcggataat
    24121 ctgactccag gaaactatat gcgcaatcgt acgctggctg atcttgatcg tgtcgttgct
    24181 ctcggcggag ggcatggact gggacgcgtt ctctcatcac tttcgtcttt gggttctcgt
    24241 ttaacgggta tcgtcaccac cacggataat ggtggctcga cggggcgtat tcgccgttca
    24301 gaaggcggca ttgcctgggg cgatatgcgc aactgcctca accaactgat aacggaaccg
    24361 agcgtcgcct ccgcgatgtt tgaataccgt tttggcggca atggcgaact ttccgggcac
    24421 aaccttggaa acttgatgtt aaaggcgctg gatcacctta gcgtgcggcc tctggaagcc
    24481 atcaatttaa ttcgtaatct gctgaaagtg gatacgcatt tgattccaat gtcagagcat
    24541 cccgtcgatc tgatggcgat tgacgatcag gggcatgaag tttacggcga ggtcaatatc
    24601 gaccagttaa ctacgccgat tcaagagtta ttattaacgc cgaatgtacc cgcaacgcgt
    24661 gaggcagttc acgctatcaa tgaagcggat ctcatcatta ttgggcctgg cagtttttat
    24721 accagcctga tgccaattct gctgctgaag gaaatcgccc aggcattacg ccgcacgcca
    24781 gcgccgatgg tttatatcgg caatctgggg cgtgagttga gtttacctgc ggctaatttg
    24841 aagctggaaa gcaagctggc aattatggag cagtatgttg gtaaaaaagt cattgatgcg
    24901 gtcatcgtcg ggccaaaagt ggatgtctcg gcggtgaaag agcggattgt gatccaggag
    24961 gtactggagg ccagcgatat tccgtatcgt catgaccgcc agttgttaca taacgcgctg
    25021 gaaaaggcgt tacaggcttt aggttaacgg aaatccgaag gaaaattccg gcttcctatt
    25081 gaagacaaag tgcgcgttgt ttatgccgga tgcggcgtga acgccttatc cggcctacaa
    25141 accgcgcaaa ttcaatatat tgcggagaaa atgtaggcct gataagcgta gcgcatcagg
    25201 ctgttttccg tttgtcatca gtcttcttcg ctatcctgtt acgatgcggc aataaacaac
    25261 tcacgcaact gatgcaactg gtcacgaatt tgtgccgctt cttcgaactc cagattctgc
    25321 gcgtgttgca tcatcagccc ttccagttca tggattttct gctgcaacgc tttcggcgac
    25381 atatccatcg gcacattatc cggctcaaca atcgggcgcg attttcctct gcccttcgct
    25441 ttggttttgg caatgttctg ccccagcgcc aggatatcga ccactttctt gttcaagcct
    25501 tgcggcgtaa tgccgtgttc ctcgttgtac ttctgctgtt tctcacggcg acgttcggtt
    25561 tcgccaatcg ctttcgccat tgatggggtg atcttatcgc cgtagagaat cgctttaccg
    25621 ttaacgttac gtgccgcacg accaatggtc tggatcaacg aacgttcgga acgcaggaag
    25681 ccttctttgt cagcgtcgag gatcgccacc agcgacactt ccggcatatc cagaccttcg
    25741 cgcagtaagt tgatccctac cagcacgtcg aactcaccca gacgcaagtc gcggataatc
    25801 tccatacgtt cgacggtgtc gatatctgag tgaagataac gcacgcgctc gccatgttct
    25861 tcgagatatt cggtaagatc ttccgccatc cgcttggtca gtgtggtgac cagtacgcgt
    25921 tcgttaattg ccgctcgctg acgaatctcc gaaagaagat catcaacctg tgtcgccacc
    25981 ggccgcactt cgataatcgg gtcaagcaat ccggttggac gcaccacctg atccaccaca
    26041 tcgccgccgg atttttccag ctcgtaatta cccggcgtcg ccgaaacata gatggtttgc
    26101 ggcgctaatg cttcgaactc ttcaaactta agcggacggt tatccagcgc tgatggcagg
    26161 cggaagccgt actccaccag tgtctcttta cgcgcccggt caccgcgata catgccgcca
    26221 atttgtggaa tggtgacgtg agattcatcg acgaccagca gcccatcggc aggcaggtaa
    26281 tcaaacagcg tcggcggtgg ctcacccggt ccacgaccgg agaggaagcg cgagtagttt
    26341 tcaatccccg aacagtagcc cagctcgttc atcatctcca gatcaaactg ggtacgctgg
    26401 gtcagccgct gctcttccag cagtttgttg ttttccaaca gcactttgcg tctggcggcc
    26461 agttcttctt tgatctcctc catcgcctgt acgatgcgct cgcgcggtgt gacgtagtgc
    26521 gttttcgggt agatggtaaa acgtggaata gtggaaacaa tctgcccggt cagcgggtca
    26581 aataacgaca atcgttccac ttcctcgtca aacagttcca cgcgaagtgc aatgtcatcc
    26641 gattctgccg ggaagatatc aatcacctcg ccacgaacgc ggaaagtacc acgctggaat
    26701 gcttgatcat tacgagcgta ttgcagctcc gccagtcggc gcagaatcgc gcgctgatcg
    26761 ataatcatac cgaccgtgag atggagcatc atcttgagat ataaatcagg atcgcccaga
    26821 ccataaatcg cggaaacaga ggccactaca accacatcac gccgctccag catcgctttg
    26881 gtggcggata aacgcatctg ctcaatatgt tcgttaaccg aggcatcttt ctcaatgaaa
    26941 gtgtcggaac tcggtacata ggcttccggc tgatagtagt cgtagtagga gacgaaatac
    27001 tccaccgcgt tttccgggaa gaactctttc atttcgccat acagctgggc cgccagcgtt
    27061 ttgttgggcg caagtaccat ggttgggcgc tgaaggtcag caatgacatt ggcaatggtg
    27121 aaggttttcc ctgagccagt cacgccaagt aacgtctggt gcgccaggcc atcttccagc
    27181 ccctcttcga gacgtcgaat cgcctctggc tgatcgccag aaggtttaaa agcggaattc
    27241 agtttgaacg gtttactcat gagtcgctac ctgaaggagt tgggcgggca ggtatgtaat
    27301 tttactcgtc gtacttaatt atgccaacaa attatactgg ataaaaaaac agttcatcac
    27361 cataatattt ctgatacagc gtaaactccg ctgtcaatca tgagcaaaat ttactctgtg
    27421 gcgagataaa actccggcct taccgggtta tccccaaagc aacggctttt ttaacatttg
    27481 tcaagatgag tgatgacagt tttatggcaa gagatgcctg ttcagtgact gccattgcat
    27541 ttatctaacc agttaaaaaa taaaagatat ttctttgagc cgtctttaac gccaattcgc
    27601 gtgcaagccg cgtattctct cgcttgcctc gtgttttcta actctaatac acatggttat
    27661 ccacaggaat agtggataac tgtctccagc ccctatcccc cgccgcctgg gcaattccca
    27721 cattcgccgc atgaggcgga agtaaaattt ttttcgtgat tattttagaa tttaattggt
    27781 taaattgcag tcaatcgaag acgcgatctc gctcgcaatt taaccaaata cagaatggct
    27841 acaacaaggc aaggtttatg tactttccgg ttgccgcatt ttctggattt tctgcaagcc
    27901 aggggatctc tcccagcagc ggcgcgggaa tcatgcgggt gagcgtggtc atatattcag
    27961 cgtgacgttt tcccggaggc gtaacatcgt tcgccaccca acccgccaga gtcagtccgg
    28021 cgtgttgtat tgcctgcgca gtcaacatcg cgtgattaat acagccgagt ttcacaccaa
    28081 ctaccagtat caccggcagt tgttcctgtg ttacccaatc tgcaaaagtg aaagtgtcag
    28141 aaagcggcgt aaaccagccg ccagcacctt ccactaacac ccagtcagcc tgttgttcaa
    28201 gcgcgcgtaa tccggcgctc attaccaatg attctatcgg cctgccctct tgcgcgctga
    28261 tgatgtgcgg cgaagtgggt tctgcgaagg tgtaaggatt tactgttgcg taatccagct
    28321 gcaggctgct gttgtgctgt aacgccagcg cgtcactatt acgtaagcct tccggggtct
    28381 tttcgctgcc agaggcgacc ggtttataac ctgccgtccg ataacctact gccttcgcgg
    28441 cttgtaaaag tgcacaactg gcgacggttt tccccacttc ggtatccgtt ccggtgacaa
    28501 aataacgttt actcacgagc aatcactccc aaaaaaagat gatacgtcag aggatatcgc
    28561 ccctgttgtt gcggccaggc cagttgcaat cgctgcaatt gcgaacgcgt taatattcgc
    28621 gggtcgcgcc cttcatgaag atgcgtagca ccgatgcctt tcagcgaacg catggcactg
    28681 agcgcatcat caaaccatag tgtgatgggc tgaatatgat gttgataatg cacgccgttc
    28741 agcgactgtt cgatttcatc tggcggtaaa aagcgattag catgcggacg ctcgtccacc
    28801 gcctgccacg cctgatgcag ttcgggtaac gatccctgca ccagcgtggt aaacgccacc
    28861 acgcctccgg ggcgcaccac ccgatacagc tcgcggagtg ccgtggataa attaccgcac
    28921 cactgcaccg cgagattgct ccatgcaaga tcgaacgtcg cagtcgctaa cggcagggat
    28981 tcgatatctc ccgccagata atggtctgca gcatctttct ggcgggcttg cacaagcatt
    29041 ggcggtgaga gatctaacgc cgtcacctgt gcgtgacgct ctcgccagtg gcggctcatc
    29101 cagcctggcc cacaccccgc gtccagtacg tgggtgtatt tacgctgtgg gagcattgcc
    29161 agtaagacgt cagcactccg gcgctgtaac tctgcgtgtt gctcatagtg cgcggctgcc
    29221 cgaccaaatg ccgctgcaat ggcttgttta ttaaccgttg ccatgcagca cctccagcag
    29281 acggtcgata tcctgcattt catgcgctgc ggttagcgtt aagcgcagtc gcgcagtacc
    29341 agcgggtacg gttggcgggc gaatcgccgt gacccagcag ccttgctggc gcagtttttc
    29401 tgccagttgt aacgcacggc tgttatcacc gacgatcaat ggctggatgg cgctgcatga
    29461 atcagcaagc gtaaacggca aatcctgtac tccggcacga aaacgcgcaa tgagtgacac
    29521 cagtttttcg cgccgtgcat ccccctcatc acggcgaatg accgccagcg acgcacgtaa
    29581 tgcctgcgcc tgagcgggcg gcatactggt gttgtagata aggtggcggg cgaattgcag
    29641 cagataatcc gccaccgtac tggagcaaag caccgctgcc ccgctgacgc caaatccttt
    29701 gccaaaagtc accaccagca attctggttt taccttttgc agccagcagc tgccgcgccc
    29761 ctgctccccg ataacgcccg tgccgtgggc atcatcgacc atcaaccagc cattgtgctg
    29821 ttgcgttacc tgctggattt ccgccagtgg cgcactatcg ccgtccatgc tgaacacgcc
    29881 ttctgtcacc accagttgct gccccggaca gggggaagca agcagtcgcg ccagatgagt
    29941 gacatcgtta tgaacaaaac ggcgaagctg cgacgggctt aaactggcag cctccagcaa
    30001 tgaggcatgg ctaagccggt cagcgacaat gcggtcctct tttgccatca tcgcggcaat
    30061 gaccgcctga ttagcggcaa aaccagagat aaatagcagt gcccgcgaat agcccagcca
    30121 ctcggccagt tcttcttcca gcgcctgatg cgcaacgcta taaccgctga cgtgaccgga
    30181 accgccgcta ccgacgccaa attgctccgc accctgctgc caggcacgga taatttgcgg
    30241 atgatggctt aaacctaaat aatcgttact ggaaaagttc agatactggc gatcatccgc
    30301 caccagccag cgtccggctc cttgcgccac tggataacgg cgacgcaggg catcggcagc
    30361 acgccgcgca tcgagcgccg cgttgatttt ctcctgccag ctcatagtgc tgccgcgttg
    30421 taatattcgt cagtatccgg ggtcatcagc gcctgttcaa gacgttgctg ttgttcgtta
    30481 tcccctgcca gcacggcagt ttgctgcgga tttagcccca gtttgcggaa cagttgcagg
    30541 tctttatctt cttccggatt cggcgtggtc agcagtttgc aaccgtagaa aatcgagttt
    30601 gcgcctgcca taaagcacat cgcctgagtc tgttcgttca tctgctcgcg tccggcagaa
    30661 aggcgcacgt aagaggtcgg catcatgatc cgcgcgaccg caatggtgcg aataaaatca
    30721 aaggcatcaa cgtcatcgtt atcggcaagc ggcgtgcctt tcaccttcac cagcatgttg
    30781 attggcacgc tttccggcgg cgtcggcagg tttgccagtt gcagcaataa tccggcgcga
    30841 tcttttaccg tttcgcctaa gcccacaatg ccgccagaac agactttgat cccggcatcg
    30901 cgcacttttt ccagcgtatc gaggcgttcc tgataagtgc gggtggtgat gatattgccg
    30961 taaaactccg gcgaggtgtc gaggttgtgg ttgtagtaat ccagcccggc gttcgcgagg
    31021 cgctgcgcct gagattcact caacgtgccc agcgtcatac acgcctccag ccctaaatct
    31081 tttaccccct gcaccatctg ctccaggtac ggcatatcgc gttcgttggg attcttccac
    31141 gccgcgccca tgcaaaaacg cgtcgatccc gccgctttcg ctttgcgcgc cgactccagc
    31201 acctgttcaa cttccatcaa ccgctcggct tccagaccgg ttttatagcg cgagctttgc
    31261 gggcaatatt tgcaatcttc cgggcaagca ccggttttaa tcgacagcaa cgtgctgacc
    31321 tgcacctgac gaggatcgaa atgctggcga tgcacctgct gcgcttcaaa cagcagatcc
    31381 agcaacggtt tttcaaataa ttctgcgact tgcgacaatg tccagcgtgg gcggtgagcc
    31441 atggggcttc tccaaaacgt gttttttgtt gttaattcgg tgtagacttg taaacctaaa
    31501 tcttttcaat ttggtttaca agtcgattat gacaaaggac gatcttgcct ttgaccaacg
    31561 ccatatctgg cacccataca catccatgac ctccccgctg ccggtttatc cggtggtgag
    31621 cgccgaaggt tgcgagctga ttttgtctga cggcagacgc ctgattgacg gtatgtcgtc
    31681 ctggtgggcg gcgatccacg gctacaatca cccgcagctt aatgcggcga tgaagtcgca
    31741 aattgatgcc atgtcgcatg tgatgtttgg cggtatcacc catgcgccag ccattgagct
    31801 gtgccgcaaa ctggtggcga tgacgccgca accactggaa tgcgtttttc tcgcggactc
    31861 cggttccgta gcggtggaag tggcgatgaa aatggcgttg cagtactggc aagccaaagg
    31921 cgaagtgcgc cagcgttttc tgaccttccg caatggttat catggcgata cctttggcgc
    31981 gatgtcggtg tgcgatccgg acaactcaat gcacaacctg tggaaaggct atctgccaga
    32041 aaacctgttt gctcccgccc cgcaaagccg catggatggt gaatgggatg aacgcgatat
    32101 ggttggcttt gcccgcctga tggcggcgca tcgtcatgaa atcgcggcgg tgatcattga
    32161 accgattgtc cagggcgcag gcgggatgcg catgtaccat ccggaatggt taaaacgaat
    32221 ccgcaaaatg tgcgatcgcg aaggtatctt gctgattgcc gacgagatcg ccaccggatt
    32281 tggtcgtacc ggcaaactgt ttgcctgtga atatgcagaa atcgcgccgg acattttgtg
    32341 cctcggtaaa gccttaaccg gcggcacaat gaccctttcc gccacactca ccacgcgcga
    32401 ggttgcagaa accatcagta acggcgaagc cggctgcttt atgcacgggc caacttttat
    32461 gggcaatccg ctggcctgcg cggctgcaaa cgccagcctg gcgattctcg aatctggcga
    32521 ctggcagcaa caggtggcgg ctattgaagt acagctgcgc gagcaacttg cccccgcccg
    32581 tgatgccgaa atggttgccg atgtgcgcgt gctgggggca atcggcgtag tcgaaacaac
    32641 tcgtccggtg aatatggcgg cgctgcaaaa attctttgtc gaacaggggg tatggatccg
    32701 gccttttggc aaactgattt acctgatgcc gccctatatt attctcccgc aacagttgca
    32761 gcgtctgacc gcagcggtta accgtgcggt gcaggatgaa acattttttt gccaataacg
    32821 ggaagtcagc gtgtgggttt ctggctacac tttctgcaaa ctagaaagga gggttcatga
    32881 aactcatcag taatgatctg cgcgatggcg ataagctgcc gcatcgtcat gtctttaacg
    32941 gcatgggtta cgatggcgat aatatttcac cgcatctggc gtgggatgat gttcctgcgg
    33001 gaacgaaaag ttttgttgtc acctgctacg acccggatgc gccaaccggc tccggctggt
    33061 ggcactgggt agttgttaat ttacccgctg atacccgcgt attaccgcaa gggtttggct
    33121 ctggtctggt agcaatgcca gacggcgttt tgcagacgcg taccgatttt ggtaaaaccg
    33181 gatacgatgg cgcagcgccg ccgaaaggcg aaacccatcg ctacattttt accgttcacg
    33241 cgctggatgt agagcatatt gatgtcgatg aaggagccag cggcgcgatg gtcgggttta
    33301 acgttcattt ccactctctg gcaagcgcat cgattaccgc gatgtttagt taatcactct
    33361 gccagatggc gcaatgctat ctggtatcac ttaaaggtat taaaaacaac tttttgtctt
    33421 tttaccttcc cgtttcgctc aagttagtat aaaaaagcag gcttcaacgg attcattttt
    33481 ctatttcata gcccggagca acctgtgaac acattttcag tttcccgtct ggcgctggca
    33541 ttggctttta gcgtgacgct gaccgcctgt agctcaacac cacccgatca acgtccttct
    33601 gatcaaactg cgcctggtac ctcttctcgc ccgattctgt cggcaaaaga agcgcagaat
    33661 ttcgatgctc aacactattt tgcatccctg acaccaggtg ctgcagcgtg gaatccttcc
    33721 ccgattaccc tgcctgcgca acctgacttt gttgtcggcc cggcgggcac tcaaggtgta
    33781 acgcatacca cgattcaggc ggcggtagat gcggcaatta tcaagcgtac caacaagcgc
    33841 cagtatattg ccgtgatgcc tggtgagtat cagggaacgg tgtatgtccc tgctgctccg
    33901 ggtggaatta ctctgtacgg tacaggtgaa aaaccgattg atgtgaagat tgggctttcc
    33961 cttgatgggg gcatgagccc tgccgactgg cgtcacgacg tcaacccgcg cggcaaatat
    34021 atgccaggta aaccagcgtg gtatatgtac gatagctgcc agagcaaacg cagcgacagt
    34081 atcggtgttc tctgctctgc ggtcttctgg tcacaaaaca atggcctgca actgcaaaac
    34141 ctgaccatcg aaaacacgct gggcgatagc gtagatgcag gtaaccatcc ggcggtggca
    34201 ctgcgtactg atggtgacca ggtacagatt aacaacgtta acattctcgg tcgtcagaac
    34261 accttctttg tcaccaacag cggtgtgcag aaccgtctgg aaacgaatcg tcagccgcgt
    34321 acgctggtga ccaacagcta cattgaaggg gatgtggata tcgtttctgg tcgcggcgca
    34381 gtggtgttcg ataacaccga attccgcgtg gtgaactcac gtactcagca agaagcgtat
    34441 gtgtttgcac cggctacgct gtccaacatt tactacggtt tcctcgccgt aaacagccgt
    34501 ttcaatgctt tcggtgatgg tgtggcgcaa ctgggccgct cgctggatgt tgatgccaat
    34561 accaacggtc aggtggtgat ccgtgatagc gccatcaacg aaggttttaa cacggctaaa
    34621 ccgtgggccg atgcggtgat ctctaatcgt ccgtttgcgg gtaataccgg cagcgtagat
    34681 gataacgacg aaatacagcg caatctgaat gacactaact acaaccgcat gtgggaatac
    34741 aataaccgcg gcgtgggtag taaagtggtt gcagaggcga agaagtaaga gcaattaact
    34801 atttgccgga tgcggcgtaa acgccttatc cggcctacgg ttcgatgcga tttgtaggtc
    34861 ggataagatg cgcaagcatc gcatccgaca ataagtgccg aatgcgacct acattcacat
    34921 ggcgcttttt acatctgacg gtttttattg aagttaatca aactacccgc cttgataatc
    34981 tcgcgctctt cagcagtcag actttccata tagagcgtaa tttccgttac cggcgcatct
    35041 tcatggatca cataaccttt aaacgtcgta cccggattat ccagcgccgc tttaatgcca
    35101 gggatgtaaa tgtaatcccc cacttcaaag gttggcactt ccgccatctg cagcggtaac
    35161 atcccccagt tgatgacgtt agaacggtag cgtttagtcg cgtactcttc ggcaatattc
    35221 gccagaccgc caatcacacg ctggcagctc gccgcctgtt cacgcgcaga accatcgcct
    35281 ggtttcaccg catataccat gctgccaatt tcagtttgca gcggatcaat atgctcctga
    35341 ccagcaatct gcttaatgcg cgcaaacacc tctgtcagct cgctgacatt ccccgccaga
    35401 cgctgatttt ccagctcagc cgtcgcttta cttctgccaa cataaccggg atcgcggcgt
    35461 gacagggtaa actccgccag accaatcgga tttgaacgat aagaagaggt ttcaccggaa
    35521 ggaatcagtt cgtcggtggt ggtcacttcg tcgaggatct tcgagcacac tttcaggacg
    35581 atattgtcag tcagcgcacc caattccggc cagtctttaa tgttcggtcc gtaaatcagc
    35641 ggttgctgag ttgccccttt cacaaagccc tgataaacac ggtttttata cggcgttaca
    35701 tcgaaggcgt actccggcac gttgtcccag caatcaagtt cgctggcaga ggttaaatag
    35761 ccaccgtttg ccgcagtcgc agcgatagaa cgagcgtcca tcaacgccac cgctgacatc
    35821 tgcccattag ctggcttaga gccttcgcgg ttcgggaagt tacgcgtggt gtggcgaata
    35881 cttaatccgt tgttgattgg cgtatcgccc gcgccaaagc atgggccgca gaacgcggtt
    35941 ctgatgattg cgcctgcgcc aatcaaatct gctaccacac cttttttggc taaatccata
    36001 aacaccggct gtgatgacgg gtaaaccgcc agcgagaagg tgtcattgcc acaggattga
    36061 ccgcgcagtg cattcgccgc cgcgatgacg ttttcgtagt taccgccaga acagcccgcg
    36121 ataatcccct gctgcacttt caggcgacca ttttccactt tatccagcag cgagagtttg
    36181 gctttaccgt gcgccacgcg ttcggactca atttcaatct cacgcagaat gtcggtcagg
    36241 ttctggttca gtgtgtcgat ttcatacacg ttgctcgggt ggaacggcag cgcaatcatt
    36301 ggtttgatgg cgcttaaatc aacgctgatg cagccatcgt agtacgccat cggttgaggg
    36361 ttaagctggc agtaatcctg gccgcgaccg tgcagcgcca gccagttatg cacttcttca
    36421 tcagtttgcc agacggagct taaacaggtg gtttccgtgg tcatcacgtc aacgctgtta
    36481 cggaaatcgg tagagagcgc gctaacgccc ggtccaacga actccatgac tttgtttttg
    36541 acgtaaccgt ttttgaacac cgcgccaatg atagccagcg ccacatcctg tggccccaca
    36601 tacggcgcag gttttccggt cagatgcacc gcaaccacgc ccggatagtc gatatcccag
    36661 gtgtcattaa gcagctgttt taccaactca ccgccgccct caccgactgc cattgtccct
    36721 aatgcaccgt aacgggtgtg gctgtctgac ccgaggatca ttttgccccc gcctgccatc
    36781 atctcacgca tatattgatg gatgatcgca atatgcggag gcacaaaaat accgccataa
    36841 cgctgagctg ccgataaacc aaaaacatgg tcatcaccgt taatggtgcc gccgacggcg
    36901 cagagtgagt tatggcagtt ggtcagcaca tacggcagcg ggaaacgttc cattccggac
    36961 gctttagcgg tctgtacaat accaacaaag gtaatatcgt gagaggctaa tgaatcaaac
    37021 ttaattttaa gtttatccat atttccggac gtattatgag aagagagaat agaccaggca
    37081 atagtgcctt ttttggcttc ttctttttta atttcgccgg tgaaatgttc ttcggcaatt
    37141 atttcgttat tactggcgag aaacacgcct ttt
//
LOCUS       DAJTYD010000019        36556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTYD010000019 DAJTYD010000000
VERSION     DAJTYD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.019"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            359..445
                     /gene="leuL"
                     /locus_tag="OO585_000877"
     CDS             359..445
                     /gene="leuL"
                     /locus_tag="OO585_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6628551.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            538..2109
                     /gene="leuA"
                     /locus_tag="OO585_000878"
     CDS             538..2109
                     /gene="leuA"
                     /locus_tag="OO585_000878"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6628552.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2109..3200
                     /gene="leuB"
                     /locus_tag="OO585_000879"
     CDS             2109..3200
                     /gene="leuB"
                     /locus_tag="OO585_000879"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6628553.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3203..4603
                     /gene="leuC"
                     /locus_tag="OO585_000880"
     CDS             3203..4603
                     /gene="leuC"
                     /locus_tag="OO585_000880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6628554.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4614..5219
                     /gene="leuD"
                     /locus_tag="OO585_000881"
     CDS             4614..5219
                     /gene="leuD"
                     /locus_tag="OO585_000881"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6628555.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPVIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5268..6350)
                     /locus_tag="OO585_000882"
     CDS             complement(5268..6350)
                     /locus_tag="OO585_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6628556.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYEDNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6451..6582)
                     /gene="sgrT"
                     /locus_tag="OO585_000883"
     CDS             complement(6451..6582)
                     /gene="sgrT"
                     /locus_tag="OO585_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6628557.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6671..8326
                     /gene="sgrR"
                     /locus_tag="OO585_000884"
     CDS             6671..8326
                     /gene="sgrR"
                     /locus_tag="OO585_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6628558.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSTLRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8360..8459
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8490..9473
                     /gene="thiB"
                     /locus_tag="OO585_000885"
     CDS             8490..9473
                     /gene="thiB"
                     /locus_tag="OO585_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6628559.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9449..11059
                     /gene="thiP"
                     /locus_tag="OO585_000886"
     CDS             9449..11059
                     /gene="thiP"
                     /locus_tag="OO585_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6628560.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11043..11741
                     /gene="thiQ"
                     /locus_tag="OO585_000887"
     CDS             11043..11741
                     /gene="thiQ"
                     /locus_tag="OO585_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6628561.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11854..12618)
                     /locus_tag="OO585_000888"
     CDS             complement(11854..12618)
                     /locus_tag="OO585_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6628562.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12704..13582)
                     /gene="araC"
                     /locus_tag="OO585_000889"
     CDS             complement(12704..13582)
                     /gene="araC"
                     /locus_tag="OO585_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6628563.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13921..15621
                     /gene="araB"
                     /locus_tag="OO585_000890"
     CDS             13921..15621
                     /gene="araB"
                     /locus_tag="OO585_000890"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6628564.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15632..17134
                     /gene="araA"
                     /locus_tag="OO585_000891"
     CDS             15632..17134
                     /gene="araA"
                     /locus_tag="OO585_000891"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6628565.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQHLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17334..18029
                     /gene="araD"
                     /locus_tag="OO585_000892"
     CDS             17334..18029
                     /gene="araD"
                     /locus_tag="OO585_000892"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6628566.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18104..20455
                     /gene="polB"
                     /locus_tag="OO585_000893"
     CDS             18104..20455
                     /gene="polB"
                     /locus_tag="OO585_000893"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6628567.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20619..23525
                     /gene="rapA"
                     /locus_tag="OO585_000894"
     CDS             20619..23525
                     /gene="rapA"
                     /locus_tag="OO585_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6628568.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23537..24196
                     /gene="rluA"
                     /locus_tag="OO585_000895"
     CDS             23537..24196
                     /gene="rluA"
                     /locus_tag="OO585_000895"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6628569.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24313..25128)
                     /gene="djlA"
                     /locus_tag="OO585_000896"
     CDS             complement(24313..25128)
                     /gene="djlA"
                     /locus_tag="OO585_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6628570.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25383..27737
                     /gene="lptD"
                     /locus_tag="OO585_000897"
     CDS             25383..27737
                     /gene="lptD"
                     /locus_tag="OO585_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6628571.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27790..29076
                     /gene="surA"
                     /locus_tag="OO585_000898"
     CDS             27790..29076
                     /gene="surA"
                     /locus_tag="OO585_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6628572.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29076..30065
                     /gene="pdxA"
                     /locus_tag="OO585_000899"
     CDS             29076..30065
                     /gene="pdxA"
                     /locus_tag="OO585_000899"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6628573.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30062..30883
                     /gene="rsmA"
                     /locus_tag="OO585_000900"
     CDS             30062..30883
                     /gene="rsmA"
                     /locus_tag="OO585_000900"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6628574.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30886..31263
                     /gene="apaG"
                     /locus_tag="OO585_000901"
     CDS             30886..31263
                     /gene="apaG"
                     /locus_tag="OO585_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6628575.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31270..32112
                     /gene="apaH"
                     /locus_tag="OO585_000902"
     CDS             31270..32112
                     /gene="apaH"
                     /locus_tag="OO585_000902"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6628576.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32190..32669)
                     /gene="folA"
                     /locus_tag="OO585_000903"
     CDS             complement(32190..32669)
                     /gene="folA"
                     /locus_tag="OO585_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6628577.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32861..34723)
                     /gene="kefC"
                     /locus_tag="OO585_000904"
     CDS             complement(32861..34723)
                     /gene="kefC"
                     /locus_tag="OO585_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6628578.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34716..35246)
                     /gene="kefF"
                     /locus_tag="OO585_000905"
     CDS             complement(34716..35246)
                     /gene="kefF"
                     /locus_tag="OO585_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6628579.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35354..36556)
                     /locus_tag="OO585_000906"
                     /pseudo
     CDS             complement(35354..36556)
                     /locus_tag="OO585_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308075.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8851 a        10034 c         9649 g         8022 t
ORIGIN      
        1 atgcattaat tattaacatt aatggatcaa taatattcac catatcaata tcaaaaaaaa
       61 ttcgcaaaac atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt
      121 gtcccctttt tcctcgttga ttagatgcaa aaatttattc tgaaatatgt caacctatga
      181 aaagtgtcgg tcgttaaaca gaaattaata tcgcttgctt taaccaccgc agcacaatta
      241 gctaatttta cggatgcaga actcgcgctg gcgggacgtt tttattgcgt cagggttgac
      301 atccgttttt gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat
      361 gactcacatc gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag
      421 acgagtgagc ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt
      481 gcgcgggttt ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg
      541 agccagcaag tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca
      601 agcttgagtg tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac
      661 gtgatggaag tcggtttccc cgtctcttcg ccgggcgatt ttgaatcagt gcaaaccatc
      721 gcccgccagg ttaaaaacag ccgcgtatgc gcgttagctc gctgcgtgga gaaagatatc
      781 gacgtggcgg ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgcc
      841 acttcgccaa tgcacatcgc caccaagctg cgcagcacgc tggatgaagt gatcgaacgc
      901 gctatctata tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa
      961 gatgccgggc gtacccccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc
     1021 ggtgccacca ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc
     1081 ggaatcatca gcggtctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta
     1141 catacccacg acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt
     1201 gcacgccagg tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg
     1261 gaagaagtca tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt
     1321 aatcaccagg agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc
     1381 ccggcaaaca aagccattgt tggcagcggc gcattcgccc actcctccgg tatccaccag
     1441 gatggcgtgc tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg
     1501 aaccaaatcc agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg
     1561 gatgagatgg ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg
     1621 aagctggcgg acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc
     1681 ggtaagcagc aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc
     1741 tctaacgata tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa
     1801 gccgccaacg gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac
     1861 tataacgtcg aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg
     1921 ggtcaggtgg atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc
     1981 accgatattg tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt
     2041 gccgcagaag tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa
     2101 accgtgtgat gtcgaagaat taccatattg ccgtattgcc gggggacggt attggtccgg
     2161 aagtgatgac ccaggcgctg aaagtgctgg atgccgtgcg caaccgcttt gcgatgcgca
     2221 tcaccaccag ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc
     2281 cgcctgcgac ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg
     2341 gtccgaaatg ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgttgcctt
     2401 tgcgtaagca cttcaaatta ttcagcaacc tgcgtccggc aaaactgtat caggggctgg
     2461 aagcattctg tccgctgcgt gctgacattg ccgctaacgg cttcgacatc ctgtgcgtgc
     2521 gcgaactgac cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacagt
     2581 atgaaaaagc gtttgatacc gaggtgtatc accgttttga gatcgaacgt atcgcccgca
     2641 tcgcgtttga atctgcccgc aagcgtcgcc acaaagtcac ctcaatcgac aaagccaacg
     2701 tgctgcaatc ctctatttta tggcgggaga tcgttaacga gatcgccacg gaatacccgg
     2761 atgtcgaact ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat
     2821 cacagtttga cgtcctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg
     2881 caatgatcac tggctcgatg gggatgttgc cttccgccag cctgaacgag caaggttttg
     2941 gtctgtatga accggcaggc ggctcagcac cagatatcac aggcaaaaac atcgccaacc
     3001 cgattgcgca aattctgtcg ctggcactgc tgctgcgcta cagcctggat gccgatgatg
     3061 cggcttgcgc cattgaacgc gccattaacc gcgcattaga agaaggcatt cgcaccgggg
     3121 atttagcccg tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct
     3181 atgtggcaga aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca
     3241 cgtagtgtac gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca
     3301 tgaagtgacc tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca
     3361 gccgggcaaa accttcgcca ctatggatca caacgtctct acccagacga aagacattaa
     3421 tgcctgtggt gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt
     3481 tggcgtcgaa ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc
     3541 ggaacagggc gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac
     3601 ccacggcgcg tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact
     3661 ggcaacgcaa accctgaaac agggccgcgc aaaaaccatg aaaattgaag tccagggcaa
     3721 agccgcgccg ggcattaccg caaaagatat cgtgctggca attatcggta aaaccggtag
     3781 cgcaggcggc accgggcatg tggtggagtt ttgcggcgaa gcaatccgtg atttaagcat
     3841 ggaaggtcgt atgaccctgt gcaatatggc aatcgaaatg ggcgcaaaag ccggtctggt
     3901 tgcaccggac gaaaccacct ttaactatgt caaaggccgt ttgcatgcgc cgaaaggcaa
     3961 agatttcgac gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt
     4021 cgataccgtt gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa
     4081 tccaggccag gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc
     4141 ggttgaacgc gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggcattcc
     4201 gctgaccgaa gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga
     4261 agatttacgc gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc
     4321 actggtggtt cccggctctg gccaggtaaa agcccaggcg gaagcggaag gtctggataa
     4381 aatctttatt gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat
     4441 gaacaacgac cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga
     4501 aggccgccag gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc
     4561 tgctgtgacc ggacatttcg ccgacattcg caacattaaa taaggagcag accatggcag
     4621 agaaatttat caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg
     4681 atgcaatcat cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc
     4741 tgtttaacga ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc
     4801 tgaacttccc gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg
     4861 gctcctcgcg tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg
     4921 cgccgagttt tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga
     4981 aattaagcga tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggtgatcc
     5041 atttcgacgt ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta
     5101 ccatcgatgc cttccgccgc cactgcatga tgaacggtct ggacagtatt gggcttacct
     5161 tgcagcacga cgacgccatt gccgcttatg aagcaaaaca acctgcgttt atgaattaat
     5221 ccccttgccc ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta
     5281 acctttgcgg ttaaaaataa tgcgacaaca gaaataaccg caattaccca gtagacagca
     5341 aagtgccccc aactttgtgc aattgctccc tgaataacgc cagccagaat taccccggta
     5401 gaaatactgt tagtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga
     5461 aaccatagca tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc
     5521 gtcatcaacg ccatacggct atgaaagaaa atcaatccgg tgtaaaacag tactcctgcc
     5581 gccactgcta tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga
     5641 atcattgctg gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct
     5701 ggcaatccta actcgctact gatccacaac ggcatatcaa taatgtacat ggtgttgcag
     5761 gtccacatta acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagccacct
     5821 tgcattgata aagcattttc cgacggcagt tctacccgcg ccacagacgg aagcataaat
     5881 gcaatcaata ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat caccacttct
     5941 cgcgccgagt tatcctcata ttcccgcgcc agagcaaata actgtggcat tgccgtattg
     6001 gccagagatg ccagaagcac accacaggtg ataagcgtca gataatgacg attaaatgca
     6061 aacaatggcg cattgccgat agccatcaaa cagcaaaata taatcagttt tcgccgatcg
     6121 ccctgactgt cagaacgttt tgccaaccag aggcttacgc cgatcccagc aatagtattc
     6181 accgtataaa agagtccgat ccagaaaggt tgcgcgccaa cctcacgact cagaaataag
     6241 ctcaatgtag gagcctgtag cgccccggcc acccccatca taaaagcgac cagcataaat
     6301 gccgcgtaaa caccgttcat acggcgagcc atcgtcatta tccagatcat acgttccctt
     6361 tttagcgcgg cgagaataaa aaaaccagca ggtataatct gctggcgggt gattttacac
     6421 caatactcag tcacacatga tgcaggcaag tcaactttca gaattgcggt catcccactg
     6481 catcagtcct tcaagcatgg ttaatcgttg tgggacgctt aaccaacgca accagcacaa
     6541 cttcgctgtc gcggtaaaat agtgctgata aaactgacgc atggggcacc tccttgcttc
     6601 atcgttagga attattgact taatataggg aaaataaaat tgctgtcttt tgcgcaggag
     6661 ttcctctttt atgccatctg ctcgtctgca acaacagttc atccgcctgt ggcaatgctg
     6721 cgagggtaaa tcgcaggaca caacgctcaa cgaactggca gcgttattga gctgctcgcg
     6781 tcgtcatatg cgtaccctgc tcaacaccat gcaggatcgc ggctggctga cgtgggaagc
     6841 ggaagtcggg cgcggtaaac gctcgcgtct gacattcctc tataccgggc tggcgcttca
     6901 gcaacagcgg gcggaagacc tgctggagca ggatcgtatc gaccaactag tgcagttggt
     6961 tggcgacaaa gcgactgtgc ggcaaatgct ggtttctcat ctgggccgca gcttccgcca
     7021 ggggcggcac atcctgcgcg tgctctacta tcgtccgttg cgtaatctgc tacctggcag
     7081 cacattgcgc cgttccgaaa cccatattgc ccggcaaatc ttcagttcgc taacgcgcat
     7141 aaatgaggaa aatggggaac tggaagcaga catcgcccac cactggcagc aaatatcacc
     7201 gcttcactgg cgtttctttt tgcgtccagg agtccatttt catcatggtc gtgaactgga
     7261 aatggacgac gtgatcgcct ctttaaaacg aatcaatacg ctgccgctct attcgcatat
     7321 tgcggacatt gtgtcgccga cgccctggac gctggatatc catctcacgc aaccggaccg
     7381 ctggttaccg ttactgctgg ggcaagttcc ggcgatgatc ctgccgcgcg aatgggaaac
     7441 cctcagtaac tttgccagcc atcccatcgg caccggtccg tatgcggtga tccgcaacac
     7501 caccaatcaa ctgaaaattc aggcattcga tgacttcttc ggttaccggg cattaatcga
     7561 tgaagttaac gtctgggttc tgccggaaat tgccgacgag ccagctggag gtctgatgct
     7621 aaaaggtcca cagggtgagg aaaaagagat tgaaagccgc ctggaggaag gttgctacta
     7681 tttactattc gacagccgca cccatcgcgg agcgaatcag caagtcaggg actgggtaaa
     7741 ctatgtgctt tctccaacta atctggtcta tttcgctgag gaacagtacc agcaactgtg
     7801 gttcccggct tatggactgc tcccccgttg gcaccatgcc cgcaccataa agagcgaaaa
     7861 accggctggc ctggaaagcc tcaccctgac cttttatcag gatcacagtg agcatcgggt
     7921 gattgccggg atcatgcagc agattctggc aagtcaccag gtcacgctgg aaatcaaaga
     7981 gatcagctac gatcagtggc atgaaggaga gatcgagagc gatatctggc ttaatagcgc
     8041 caactttacg ctgccgctgg atttttcgct gttcgcgcac ttgtgcgagg tgccgctgct
     8101 ccaacactgt attccgatcg actggcaagc cgacgccgcc cgctggcgca atggcgaaat
     8161 gaacctggcg aactggtgcc agcaactggt cgccagtaaa gcaatggtgc cgcttatcca
     8221 ccactggctg atcattcagg gacaacgcag tatgcgcggc ctgcgtatga ataccctcgg
     8281 ctggttcgat tttaaatcag cgtggtttgc gccgccggat ccatgattgc tggtagataa
     8341 caaaatcact acactaacgc cgttctcaac ggggtgccac gcaaacgcgt gcgctgagaa
     8401 aatacccgtc gaacctgatc cggataacgc cggcgaaggg atttgaggct ccttctcaag
     8461 tcctttgcca ctcttttttg aggtgcaaag tgttaaaaaa atgtcttccc ctgctgttgc
     8521 tgtgcacagc gcccgttttc gctaaacccg ttctgactgt ttatacctac gattccttcg
     8581 ccgccgactg ggggcctggt ccaaagatca aaaaagcttt tgaagccgac tgtaactgcg
     8641 aactgaaact ggtggcgctg gaagatggcg tttcgcttct caaccgtcta cggatggaag
     8701 gcaaaaatag taaagccgat gtggtgctgg ggctggataa caatctgtta gacgccgcca
     8761 gtaaaaccgg actatttgcc aaaagcggtg tggcagcgga tgccgttaac gttcccggcg
     8821 gctggaataa tgacactttc gtcccgtttg attacggcta tttcgccttc gtctatgaca
     8881 agaacaaact gaaaaacccg ccgcaaagcc tgaaagaatt ggttgagagc gatcaaaact
     8941 ggcgggtgat ttatcaggat ccgcgcacca gtacgccggg gctgggcctg ctgttgtgga
     9001 tgcaaaaagt ctatggcgat gacgccccac aagcctggca aaaactggcg aagaaaaccg
     9061 tcacggtcac caaaggctgg agcgaagcct acggcctgtt tttaaaaggt gaaagcgatc
     9121 tggtactgag ttacaccacc tctccggctt atcacattct cgaagagaaa aaagataact
     9181 acgccgccgc gaactttagc gaaggtcact atctgcaggt ggaagtcgcc gcccgcaccg
     9241 ctgccagcaa gcagccggag ctggcgcaaa aattcctcca gtttatggtt tctccggctt
     9301 ttcagaatgc gatcccaacc ggtaactgga tgtatccggt ggcaaatgtc acgctgcctg
     9361 ccgggtttga acaattgacc aaaccggcca ccacgctgga gttcacacca gctgaagtgg
     9421 cggcacaacg tcaggcatgg attagcggat ggcaacgcgc cgtcagccgt taattcccgg
     9481 ctggttaatt ccaggtgtaa gcgccgccac gctggtggta gcggttgcgc tcgcggcgtt
     9541 tctcgccctg tggtggaacg cgccgcaggg taactgggtg gcagtctggc aggacagcta
     9601 cctgtggcat gtggtgcgct tctccttctg gcaggcgttt ctctcggcgc tactctctgt
     9661 cgtacccgcg atattcctcg cccgcgccct ctatcgcagg cgctttccgg gtcggctggc
     9721 gctgttgcgt ctgtgcgcaa tgaccttgat cctcccggtg ctggttgctg ttttcggcat
     9781 tcttagcgtc tatggtcgcc agggctggct ggcatcgctc tgccaatcgc tcggtctgga
     9841 gtggaccttt tcgccctacg gcctgcaagg tattttgctg gcgcacgtat tttttaatct
     9901 gccgatggcg agccgcttat tactccaggc actggaaaac attcccgacg aacaacgtca
     9961 gcttgccgcc cagcttggga tgcgcggctg gcattttttc cgcttcgtcg aatggccgtg
    10021 gttacggcga caaatcccgc cggttgctgc acttatcttt atgctctgtt tcgccagctt
    10081 cgccaccgtg ctatcactgg gcggcggtcc gcaggcgacc actatcgagc tggctattta
    10141 tcaggcgctg agttacgact acgatcctgc ccgcgcggcg atgctggcgc tgatccagat
    10201 ggtgtgttgc cttgggctgg tactgttgag tcagcgattg agtaaggcca ttgcgccagg
    10261 caccacgctg ctgcaaggct ggcgcgaccc ggacgatcgt ctgcatagcc gcatttgcga
    10321 cacggtgtta atagtgctgg cgttgctgct gttgttgcca ccgttgctgg cggtgatcgt
    10381 cgatggggta aatcgccagt tgccggaagt gctggcacaa ccggtgctgt ggcaggcgct
    10441 gtggacctcg ttgcgtattg cgctggcggc aggtgtattg tgcgtagtgc tgaccatgat
    10501 gctgctatgg agcagtcgcg aacttcgggc acggcagaaa atgctggcgg gtcaggcgct
    10561 ggagatgagc ggcatgttga ttctcgccat gccggggatt gtgctggcta ccggcttctt
    10621 tttactgctc aacaacacta tcggcctgca acaatctgct gacggcattg tgattttcac
    10681 caatgcgtta atggcgatcc cttatgcgct gaaagtactg gaaaacccga tgcgcgatat
    10741 caccgcccgc tacagtatgt tgtgtcagtc gctgggcatt gaaggctggt cgcgcttaaa
    10801 agtggtggag ctgcgcgccc tgaaacgtcc actggcgcag gcgctggcct ttgcctgcgt
    10861 gctgtcgatt ggtgattttg gcgtggtggc gttgttcggt aacgatgatt tccgtaccct
    10921 gccgttttat ctctaccagc aaattggctc ctatcgcagc caggacggcg cggtcaccgc
    10981 gttaattctg ctgctactct gttttctgct gtttaccgtg attgaaaaac taccggggcg
    11041 aaatgttaaa actgactgat atcacctggc tttaccaaca tttgccgatg cgttttagct
    11101 taacggtgga acgcggcgag caggtggcga tcctcgggcc aagcggcgca ggtaaaagta
    11161 ccctgctgaa tttgattgcc ggttttctga cgccagccag cggttcgctg actatcgatg
    11221 gcgttgatca caccacgacg ccgccgtcac gccgtccggt gtcgatgctg tttcaggaga
    11281 acaacctgtt cagccacctg acggtcgcgc agaacatcgg gctggggctt aatccaggat
    11341 tgaagctgaa cgcggcacag caggagaaaa tgcacgctat cgcccgtcag atggggattg
    11401 ataatttaat ggcgcggtta ccgggcgagc tttccggcgg ccagcgacag cgcgtggcgt
    11461 tagcgcgttg tctggtgcgc gaacagccga ttttattgct cgatgaaccg ttctctgcgc
    11521 tcgatccggc gttacgtcag gagatgctga cgctggtgag cacgagctgc cagcagcaaa
    11581 aaatgacgct attgatggtg tcgcacagtg tggaagatgc ggcgcggatc gccacacgct
    11641 cggtggtggt cgccgacggg cgcatcgcct ggcagggtaa gactgaagaa ttgttgagcg
    11701 gtaaggcgag tgcttcggca atattgggga ttacgggtta gtatgcactg tcagatgcgg
    11761 cgtgaacacc ttatccgacc tacggacagt gcgttttgta ggcctgataa gaccggtcag
    11821 cgtcgcatca ggcaacccgc acaagacaat cccctaaacc ccaaccactt tacgcaaaat
    11881 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
    11941 ggcagaaatc aacggcgtca gccacaacaa acggccgcgc gacaaataat gacttaaacg
    12001 atcggttgct ttaccgctgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
    12061 taccgccgtc gccagcagca accatttaaa ctcgccgctc tgcattccgg ctggaatatc
    12121 gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac ggtggccaca gcaggcagcc
    12181 gataatattc ggcgtaatga attttgccac cggcagatcc agcattcccg ccaccattgg
    12241 caccagcgga cgcgtcgggc caacaaagcg accgaccaga atggtgaaca tgctgtgttg
    12301 atgcaacgca tgttcagttt tatcgagtaa tgctttgttt ttcttcagaa atgaccagcg
    12361 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcagaca
    12421 accgacaatc cctgccagcc aggcgtgcca gaaacttaac tcgccgctgc caatcagcgc
    12481 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
    12541 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtagattggg taataaagtg
    12601 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
    12661 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
    12721 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
    12781 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
    12841 tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct
    12901 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
    12961 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
    13021 cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg agcgactcgt
    13081 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
    13141 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
    13201 gctggtgcgc ttcatccggg cgaaagaaac ccgtattggc aaatattgac ggccagttaa
    13261 gccattcatg ccaataggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
    13321 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
    13381 gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga atggtgagat
    13441 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
    13501 cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc ggcagcaggg
    13561 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
    13621 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
    13681 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
    13741 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
    13801 gcgtcacact ttgctattcc atagcatttt tatccataag attagcggat cctacctgac
    13861 gctttttatc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
    13921 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggac
    13981 tgcgccagcg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcaaaaaggg
    14041 caattttgtg atgccccgaa taaccagttc cgtcatcatc cgcgtgacta cattgagtca
    14101 atggaagcgg cactgaaaac cgtgcttgca gagcttagcg tcgaacagcg cgcagctgtg
    14161 gtcgggattg gcgttgacag taccggctcg acgcccgcac cgattgatgc cgacggtaac
    14221 gtgctggcgc tgcgcccgga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
    14281 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
    14341 gttgactact cccgctacat tggtggtatt tattccagcg aatggttctg ggcaaaaatc
    14401 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
    14461 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
    14521 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
    14581 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
    14641 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
    14701 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
    14761 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
    14821 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
    14881 gatggcagcg tggtacctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
    14941 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
    15001 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
    15061 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggc
    15121 cgccgcacac cgaacgctaa ccaacgcctg aaaggggtga ttaccgatct taacctcgct
    15181 accgacgctc cgctgctgtt tggcggtttg attgctgcca ccgcctttgg cgcacgcgca
    15241 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    15301 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    15361 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    15421 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    15481 accctgcaac cgcgcagcga acagacacaa cgctttgaac agctttatcg ccgctatcag
    15541 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    15601 caggccgttc cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    15661 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    15721 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    15781 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    15841 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    15901 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    15961 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    16021 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    16081 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgtcagg cggtctctaa
    16141 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    16201 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    16261 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    16321 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    16381 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    16441 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    16501 tctggccgta cagcatctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    16561 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    16621 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    16681 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    16741 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    16801 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    16861 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    16921 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    16981 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgt cagttcgccg agatgcacga
    17041 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    17101 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    17161 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    17221 aaagcgcatc cggcattcaa cgcctgatgc gacgctggcg cgtcttatca ggcctacgcg
    17281 ctgcgatttt gtagaccgga taagcgaagc gcatccggca cgaaggagtc aacatgttag
    17341 aagatctcaa acgccaggta ttagaagcca acctggcgct gccaaaacac aacctggtca
    17401 cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg cggcgtcttt gtgatcaaac
    17461 cttccggcgt cgattacagc gtcatgaccg ctgacgatat ggtcgtggtt agcatcgaaa
    17521 ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga cacgccaact caccggctgc
    17581 tctatcaggc attcccctcc attggcggca ttgtgcatac gcactcgcgc cacgccacca
    17641 tctgggcgca gtcgggtcag tcgattccag caaccggcac cacccacgcc gactatttct
    17701 acggcaccat tccctgcacc cgcaaaatga ccgacgcaga aatcaacggc gaatatgagt
    17761 gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca gggtatcgat gcagcgcaaa
    17821 tgcccggcgt tctggtccat tcccacggcc cgtttgcatg gggcaaaaat gccgaagatg
    17881 cggtgcataa cgccatcgtg ctggaagagg tcgcttatat ggagatattc tgccgtcagt
    17941 tagcgccgca gttaccggat atgcagcaaa cgctgctgga taaacactat ctgcgtaagc
    18001 atggcgcgaa ggcatattac gggcagtaat gactgtataa aaccacagcc aatcaaacga
    18061 aaccaggcta tactcaagcc tggttttttg atggattttc agcgtggcgc aggcaggttt
    18121 tatcttaacc cgacactggc gggacacccc gcaagggaca gaagtctcct tctggctggc
    18181 gacggacaac gggccgttgc aggttacgct tgcaccgcaa gagtccgtgg cgtttattcc
    18241 cgccgatcag gttccccgcg ctcagcatat tttgcagggt gaacaaggct ttcgcctgac
    18301 accgctggcg ttaaaggatt ttcaccgcca gccggtgtat ggcctttact gtcgcgccca
    18361 tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt ggcgttaccg tctacgaggc
    18421 cgatgtgcgc ccgccagaac gctatctgat ggagcggttt atcacctcac cggtgtgggt
    18481 cgagggtgat atgcacaatg gcactatcgt taatgcccgt ctgaaaccgc atcccgacta
    18541 tcgtccgccg ctcaagtggg tttctataga tattgaaacc acccgccacg gtgagctgta
    18601 ctgcatcggc ctggaaggct gcgggcagcg catcgtttat atgctggggt cggagaatgg
    18661 cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc agccgcccgc agttgctgga
    18721 aaaactcaac gcctggtttg ccaactacga tcctgatgtg atcatcggtt ggaacgtggt
    18781 gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt taccgtcttc cgctgcgtct
    18841 tgggcgcgat aatagcgagc tggagtggcg cgagcacggc tttaaaaacg gcgtcttttt
    18901 tgcccaggct aaaggtcggc taattatcga cggtatcgag gcgctgaaat ccgcgttctg
    18961 gaatttctct tcattctcgc tggaaactgt cgctcaggag ctattaggcg aaggaaaatc
    19021 tatcgataac ccgtgggatc gaatggacga aattgaccgc cgtttcgccg aagataaacc
    19081 tgcgctggca acttataacc tgaaagattg cgagctggtg acgcagatct tccacaaaac
    19141 tgaaatcatg ccatttttac tcgaacgggc aacggtgaac ggcctgccgg tggaccgaca
    19201 cggcggttcg gtggcggcat ttggtcatct ctattttccg cgaatgcatc gcgctggtta
    19261 tgtcgcgcct aatctcggcg aagtgccgcc gcacgccagc cctggcggct acgtgatgga
    19321 ttcacggcca gggctttatg attcagtgct ggtgctggac tataaaagcc tgtacccgtc
    19381 gatcatccgc acctttctga ttgatcccgt cgggctggtg gaaggcatgg cgcagcctga
    19441 tccagagcac agtaccgaag gttttctcga tgcctggttc tcgcgagaaa aacattgcct
    19501 gccggagatt gtgactaaca tctggcacgg gcgcgatgaa gccaaacgcc agggtaacaa
    19561 accgctgtcg caggcgctga aaatcatcat gaatgccttt tatggcgtgc tcggcaccac
    19621 cgcctgccgc ttcttcgatc cgcggctggc atcgtcgatc accatgcgtg gtcatcagat
    19681 catgcggcaa accaaagcgt tgattgaagc acagggctac gacgttatct acggcgatac
    19741 cgactcaacg tttgtctggc tgaaaggcgc acattcggaa gaagaagcgg cgaaaatcgg
    19801 tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa acgctgcaaa aacaacggct
    19861 gaccagcgca ttagaactgg agtatgaaac ccatttctgc cgttttctga tgccaaccat
    19921 tcgcggagcc gataccggca gtaaaaagcg ctacgccggg ctgattcagg agggcgataa
    19981 acagcggatg gtgtttaaag ggctggaaac cgtgcgcacc gactggacgc cgctggccca
    20041 gcagtttcag caggagctat acctgcgcat cttccgcaac gagccatatc aggaatatgt
    20101 acgcgaaacc atcgacaaac tgatggcggg tgaactggat gcgcgactgg tttaccgtaa
    20161 acgccttcgc cgtccgctga gcgagtatca gcgtaatgtg ccgcctcatg tacgcgccgc
    20221 tcgccttgcc gatgaagaaa accaaaagcg tggtcgcccc ttgcaatatc agaatcgcgg
    20281 caccattaag tacgtatgga ccaccaacgg cccggagccg ctggactacc aacgttcacc
    20341 actggattac gaacactatc tgacccgcca gctacaaccc gtggcggagg gaatactccc
    20401 ttttattgag gataattttg ctacacttat gaccgggcaa cttgggctat tttgagcaaa
    20461 aaaagagttc gccagatacc attttgatgc gtgacgaatg ctttgccatc cagtaccata
    20521 gcgccctttc cattcctgga cctgaataac accactacct cataagcacg gtagcgggtg
    20581 gttattgcct gcaattaaag atatagagcc gaacacatat gccttttaca cttggtcaac
    20641 gctggatcag cgatacagaa agcgaattgg gacttggaac cgttgtcgcg gtggatgcgc
    20701 gaactgtcac tttacttttc ccatctactg gtgaaaaccg tctgtacgca cgcagtgatt
    20761 cccccgtgac ccgcgtgatg ttcaaccctg gtgataccat taccagccat gacggctggc
    20821 agatgcaagt cgaagaagta aaagaagaaa atggcttgct gacctatatc ggtactcgcc
    20881 tggatactga agagtccggc gtagccctgc gtgaagtttt ccttgatagc aaactggtgt
    20941 tcagcaaacc gcaggaccgc ctgtttgccg ggcagattga ccgtatggac cgctttgcgc
    21001 tgcgttatcg cgcgcgtaag tattccagcg aacagttccg tatgccgtac agcggcctgc
    21061 gcggtcagcg taccagcctg atcccgcacc agctcaacat cgctcatgat gttggccgcc
    21121 gccacgcgcc gcgcgtcctg ctggctgacg aagtgggttt agggaaaacc attgaagccg
    21181 ggatgatcct gcatcagcaa ctgctctctg gcgctgctga acgtgtgctg attatcgtcc
    21241 cggaaacctt acagcatcag tggctggtag aaatgctgcg ccgtttcaac ctgcgctttg
    21301 ctctgtttga tgatgagcgt tatgccgaag ctcagcacga tgcttacaac ccgttcgaca
    21361 ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg tagcaaacag cgcctggaac
    21421 atctctgtga agccgaatgg gacctgctgg tggtcgatga agcgcatcac ctggtgtgga
    21481 gcgaagatgc gccaagccgt gaatatcagg ccattgaaca actggcagag cacatgccgg
    21541 gcgttctgct gctgaccgcg accccggaac agctggggat ggaaagccac ttcgcccgtc
    21601 tgcgtctgct ggacccgaac cgtttccacg attttgcgca gttcgttgaa gagcagaaaa
    21661 attatcgtcc ggttgcggac gccgttgcca tgctgctggc aggtaacaaa ctgagcaatg
    21721 acgaactgaa catgctcggc gagatgatcg gcgagcagga tatcgagccg ctgttgcagg
    21781 cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca ggagctggtt tcgatgctga
    21841 tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac gcgtaacggt gtgaaaggat
    21901 tcccgaaacg cgagctgcac accattaagc tgccgctacc gacgcagtat cagacggcta
    21961 ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga agatcgtgct cgcgatatgc
    22021 tctacccgga gcgtatttat caggaatttg aaggtgataa cgccacctgg tggaacttcg
    22081 atccgcgcgt tgagtggctg atgggctacc tgaccagcca tcgctctcag aaagtgctgg
    22141 tgatctgcgc caaagctgcc actgcgctgc aactggagca ggtactgcgc gaacgtgaag
    22201 gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat cgaacgtgac cgcgctgccg
    22261 cctggtttgc cgaagaagac accggcgcac aggtactgct gtgctcagaa atcggttctg
    22321 aaggacgtaa cttccagttc gccagccaca tggtgatgtt tgacctgcca ttcaacccgg
    22381 atctactgga gcagcgtatt ggtcgtctgg atcgtatcgg ccaggcgcac gatattcaga
    22441 tccatgtgcc ttatctggag aaaaccgctc agtcggtgct ggtgcgctgg tatcacgaag
    22501 gtctggatgc atttgagcac acctgcccga ccggacgcac tatttacgat agcgtataca
    22561 acgatctgat taactatctg gcttcaccgg atcaaaccga aggctttgac gatctgatca
    22621 aaaactgccg cgagcaacat gaagcgctga aagcacagct ggaacagggt cgtgaccgcc
    22681 tgctggaaat ccactccaac ggtggcgaaa aagcccaggc actggcagaa agcattgaag
    22741 agcaggatga cgataccaac ctgatcgcct tcgccatgaa cctgctcgat attatcggta
    22801 tcaatcagga cgatcgcggc gacaacatga tcgtgctgac gccgtccgat catatgctgg
    22861 tgccggactt ccctggcctg tcggaagatg gcatcaccat cacctttgat cgtgaagtgg
    22921 cgctggcgcg tgaagatgca cagtttatta cctgggagca tccgctgatc cgcaacggtc
    22981 tggatctgat cctttctggc gataccggta gcagcacgat ttcactgtta aaaaacaaag
    23041 cgttgccggt aggtacgctg ttggtggaac tgatttatgt ggttgaagcc caggctccga
    23101 agcagttgca gctcaaccgc ttcctgccac cgacgccggt acgtatgctg ctggataaaa
    23161 acggcaacaa cctggcggcg caggtagagt ttgaaacctt taaccgccag cttaacgcgg
    23221 ttaaccgtca caccggcagc aaactggtta acgccgtgca gcaggatgtt cacgctatcc
    23281 ttcaactggg tgaagcgcag atcgagaaat ctgcccgtgc attgattgat gcagcgcgta
    23341 acgaagccga cgaaaaactg tctgccgagc tgtctcgtct ggaagctctg cgtgcagtga
    23401 acccgaacat tcgtgacgac gaactgaccg ccattgagag caaccgtcag caggtaatgg
    23461 aaagcctgga tcaggcaggt tggcgtctgg atgccctgcg tttgatcgtt gtaacgcatc
    23521 agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca ccgcaggaac cctggttggt
    23581 tatcctgtat caggatgacc acattatggt ggttaacaag ccgagcggtt tgttgtctgt
    23641 gccggggcgt ctggaagagc acaaagacag cgtgatgacg cgcattcagc gtgattatcc
    23701 gcaggcagaa tcggtgcatc gtctggatat ggctaccagc ggcgtgattg tagtggcgct
    23761 gaccaaagcc gcggagcggg agttaaaacg ccagttccgc gagcgcgagc cgaaaaagca
    23821 gtatgtggcc cgcgtctggg ggcatccatc ccctgcggaa ggtctggtgg atctgccgct
    23881 gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt tacgaaacgg gtaaacctgc
    23941 gcagacggaa tatgaagtgg tggagtatgc ggcggataac acggcaagag tggtgttaaa
    24001 accgattact gggcgttcgc atcaattgcg tgtgcatatg ctggcgctgg ggcatccgat
    24061 tctcggcgat cgtttttatg catcaccaga agcgagagcg atggcaccgc gtttgttgct
    24121 acatgcagag atgttgacga ttacccatcc ggcgtatggc aatagtatga cgtttaaagc
    24181 gccagcggat ttttaatagt cacgctggcg cagttcggta ggcggataag gcgttcacgc
    24241 cgaatccggc aattgtgcgc cgattgcctg atgcgacgcg ggccgcgtct tatcaggcct
    24301 acagtttaaa acttatttaa accctttctg ctgctttatc agctcatatg cctgctgaat
    24361 ttcctgcgct ttctgcttcg ccatctccat catctcaggc ggcaaacctt tcgccaccag
    24421 cttatcggga tggtgttcac tcatcagctt acggtaggct cgtttaatgg tggtcgcatc
    24481 atccgtcggc ttcacaccca gcacattaca ggcatcttcc agcgtcgggc cacgctgcgc
    24541 ttgctgccag ttaccaccgc cagattgctg ctgataaccg ccgccaaact gtgcaccgcc
    24601 ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag atccctaatt cttctgcaat
    24661 gacatacagc accgcccgtt cattcgggtg cagtgaacca tcagcaaacg ctgcctgaat
    24721 ctggatctcc agaaacatac gaattaagtc aaaacgacca aagcagacac tgcgaaactg
    24781 gcgcatcttt tcgcgcagcg ggtaattatc tgattttccc acccggaacg cgttttgcgc
    24841 cgcagtacgg gaagcgccat gaaggttcat tcggtccatc agctggctgg cgatatgaat
    24901 atcagcctcc gtgacgcgac ctttggattt ggttaaatgc cccatcactt caaaagtggt
    24961 ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac cacgccattt tacggctacg
    25021 ggctttatca aacatatggc caattaacag gcctaacact acgccccaaa agccgccgcc
    25081 catcagtaag gccacggcca cgccaatgat ttttccccaa tactgcatat attccccaaa
    25141 tcgacacacg gatatcaggg ctatctccca caatataaag gtgcttttac cgttttccgg
    25201 ctgcagtcaa ttgtgggaca tcgcctataa tttgcattat catacctgtc attcaatgcc
    25261 gtgcctaaca ccactgacgc tattcggaca ggattaacac tagcgtagag atgacgagta
    25321 cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac
    25381 gtatgaaaaa acgtatcccc actctcctgg ccaccatgat tgccaccgcc ctttatagtc
    25441 aacagggact ggcagccgac ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc
    25501 gtcctctggt acagggcgat accaatgact tacccgtgac tatcaatgct gaccacgcga
    25561 aaggggacta cccggatgac gccgtgttta ctggcagcgt ggatatcatg cagggtaaca
    25621 gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga ggcaccagga caaccggagc
    25681 cggtacgaac cgttgatgcg ctcggtaatg tccattacga cgataaccag gtgatcctca
    25741 aagggccgaa aggctgggcg aatctgaaca ccaaagatac caacgtctgg gaaggtgatt
    25801 accagatggt gggtcgccag ggccgcggta aagcggacct gatgaaacaa cgtggtgaaa
    25861 accgctatac cattctggat aacggtagct ttacctcctg tctgccgggt tctgacacct
    25921 ggagcgtggt aggtagcgaa attattcatg accgcgaaga acaagttgcg gagatctgga
    25981 acgcccgctt taaggtgggt ccagtaccga tcttttatag cccctatttg cagttgccgg
    26041 tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc caagtacacc accaccaact
    26101 actttgagtt ctacctgcca tattactgga acatcgcgcc aaatatggat gccaccatca
    26161 cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga gaacgaattc cgctacctct
    26221 cccaggcggg cgctggcttg atggaactgg actatctgcc ttcagataaa gtctatgaag
    26281 atgaacaccc gaacgatgac agttcacgtc gttggttgtt ctactggcaa cactccgggg
    26341 tcatggatca agtgtggcgt ttcaacgtag actacaccaa ggtcagcgat cctagctact
    26401 tcaatgattt cgataacaag tacggttcca gtactgacgg ctacgcaacg caaaaattca
    26461 gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc aaccaagcag ttccaggtct
    26521 ttagcgagca gaacaccagt agctactcgg cagagccgca gttagacgtt aactactacc
    26581 agaatgatgt tggtccgttt gatacgcgta tttacggcca ggcagtgcac tttgttaaca
    26641 ccagagacga catgcctgaa gcaacccgtg ttcacctgga accgaccatc aatttgccgc
    26701 tctctaataa ctggggcagc atcaataccg aagcgaagtt gctggcaacc cattatcagc
    26761 aaaccaatct tgactggtat aactccagaa acacgaccaa gctggacgaa tccgttaacc
    26821 gcgttatgcc gcaattcaaa gttgacggca aaatggtctt tgaacgcgat atggaaatgc
    26881 tggctccggg ttatacccaa acgctggaac cgcgcgcgca gtatttgtac gtgccgtatc
    26941 gcgatcagag cgacatctat aactacgact cgtctctact gcaatctgac tactctggcc
    27001 tgttccggga ccggacttac ggcggtcttg accgtattgc ctccgctaac caggtgacga
    27061 ccggtgtcac atctcgcata tatgatgatg ctgccgttga acattttaat atttccgttg
    27121 gtcaaatcta ctatttcacg gagtctcgca ctggcgatga caacataaca tgggagaatg
    27181 acgacaaaac gggctcactg gtgtgggcag gcgatactta ctggcgtatc tccgagcgtt
    27241 ggggattgcg tggcgggatt cagtacgata cacgtctgga taacgtagcg accagtaact
    27301 ccagcattga ataccgtcgg gatgaagacc gtctggtaca gctgaattac cgttacgcca
    27361 gcccggaata tattcaggct acgctgccta agtactattc cactgctgag caatataaga
    27421 atggtatttc gcaggtaggt gctgtcgcca gctggccaat tgccgatcgt tggtccattg
    27481 ttggggccta ctactacgac accaatgcga acaagcaagc cgactctatg ttaggtgtgc
    27541 aatacagctc ctgctgctat gcaattcgcg tcggttacga gcggaagctg aacggttggg
    27601 ataacgataa acaacatgcg gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg
    27661 gcctgagctc caactacggt ctgggtacgc aagagatgct gcgttcgaac attctgccgt
    27721 atcaaaacac tttgtgattt gttgatttac cacgtaatcc gcagtgcggt taattgaaat
    27781 ggaaaaagta tgaagaactg gaaaacgctg cttctcggta tcgccatgat cgcgaatacc
    27841 agtttcgctg ccccccaggt agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg
    27901 ctggaaagcg acgttgatgg attaatgcag tcggtaaaac tgaacgctgc gcaggcaagg
    27961 cagcagcttc ctgatgacgc gacgctgcgc caccaaatca tggaacgttt gatcatggat
    28021 caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa tctccgatga gcagctggat
    28081 caggcgattg ctaacatcgc gaaacagaac aacatgacgc tggatcagat gcgcagccgt
    28141 ctggcttacg atggtctgaa ctacaacacc tatcgtaacc agatccgcaa agagatgatt
    28201 atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca ccatcctgcc gcaggaagtc
    28261 gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca gcactgagct gaacctgagc
    28321 cacatcctga tcccgctgcc ggaaaacccg acttctgatc aggtgaacga agcggaaagc
    28381 caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt
    28441 gctcattctg ccgaccagca ggcgctgaac ggcggccaga tgggctgggg ccgtattcag
    28501 gagttgccgg gtatcttcgc ccaggcatta agcaccgcga agaaaggcga cattgttggc
    28561 ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta acgacctgcg cggcgaaagc
    28621 aaaaatatct cggtgaccga agttcatgct cgccatattc tgctgaaacc gtcgccgatc
    28681 atgactgacg aacaggcccg tgtgaaactg gaacagattg ctgccgatat caagagtggt
    28741 aaaacgactt ttgctgccgc agcgaaagag ttctctcagg atccaggctc tgctaaccag
    28801 ggcggtgatc tcggctgggc tacaccagat attttcgatc cggccttccg tgacgccctg
    28861 acccgcctga acaaaggtca aatgagtgca ccggttcact cttcattcgg ctggcattta
    28921 atcgaactgc tggatacccg taatgtcgat aaaaccgacg ctgcgcagaa ggatcgtgca
    28981 taccgcatgc tgatgaaccg taagttctcg gaagaagcag caagctggat gcaggaacaa
    29041 cgcgccagcg cctacgttaa aatcctgagc aactaatggt taaaacccaa cgtgttgtga
    29101 tcactcccgg cgagcccgcc gggattggcc cggacttagt tgtccagctt gcacagcgtg
    29161 agtggccggt cgaactggtt gtttgtgccg atgccactct ccttaccgac cgggcagcga
    29221 tgctcggttt gccgctcacc ctccgccctt attcccccaa ctcccctgca caaccgcaaa
    29281 ctgcgggcac attaacgcta cttcctgtcg cgctacgcga atctgtcact gcggggcagt
    29341 tagcgattga aaatgggcat tacgtggtgg agacgctggc gcgagcgtgt gatggctgtc
    29401 tgaacggtga atttgctgcg ctgatcacag gccccgtgca taaaggcgtc attaacgacg
    29461 caggtattcc gtttactggt cataccgagt ttttcgaaga gcgctcgcag gcgaaaaaag
    29521 tggtgatgat gctggcgacc gaagaacttc gcgtggcgct ggcaacgacg catttaccgc
    29581 tgcgcgatat cgcagatgct atcacccctg cacttttgca cgaagtgatt gctattttgc
    29641 atcacgattt gcgtacaaaa tttggtattg ccgaaccgcg cattctggtc tgcgggctga
    29701 atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga gatagacacc attattccgg
    29761 tgctcgacga gctgcgggcg caggggatga tactcaacgg gccgctgcct gccgataccc
    29821 tgtttcagcc gaaatatctc gataacgccg acgccgtgct ggcgatgtac cacgatcagg
    29881 gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc
    29941 cctttattcg cacatcagtg gaccacggca ccgcgcttga actggcggga cgtggcgaag
    30001 ccgatgtcgg cagttttatt acggcgctta atctcgccat caaaatgatt gttaacaccc
    30061 aatgaataat cgagtccacc agggccactt agcccgtaaa cgcttcgggc aaaactttct
    30121 caacgatcag ttcgtgatcg acagcattgt ttctgccatt aacccgcaaa agggccaggc
    30181 aatggtcgaa atcggccccg gtctggcggc attgaccgag ccggtcggcg aacgtctgga
    30241 ccagctgacg gtcatcgaac ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt
    30301 cttaggcccg aaactgacga tttatcagca ggatgcgatg acctttaact ttggcgaact
    30361 ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac ctgccttata acatctccac
    30421 gccgttgatg ttccatctgt ttagctatac tgatgccatt gccgacatgc actttatgtt
    30481 gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac agcaaagcgt atggtcgatt
    30541 aagcgtcatg gcgcaatact attgcaacgt gatcccggta ctggaagttc cgccgtcagc
    30601 ctttacacca ccacccaaag tggattccgc cgtcgtgcgc ctggttcctc atgcaacgat
    30661 gcctcacccg gttaaagatg ttcgtgtgtt gagccgcatc accaccgaag cctttaacca
    30721 gcgtcgtaaa accattcgta acagcctcgg caacctgttt agcgtcgagg tgttaacggg
    30781 aatggggatc gacccggcga tgcgagcgga aaatatctct gtcgcgcaat attgccagat
    30841 ggcgaactat ctggcggaga acgcgccttt gcaggagagt taacgatgat caattcgccc
    30901 cgagtgtgta ttcaggttca aagcgtctac attgaggcac aatcttcacc tgataatgaa
    30961 cgttacgttt ttgcttatac cgtaaccata cgcaatctgg ggcgagcgcc agtgcagttg
    31021 ttggggcgtt actggctgat caccaatggc aatggccgtg aaaccgaagt ccagggcgaa
    31081 ggagtggttg gcgtccagcc acttatcgcg cctggcgaag agtaccagta caccagcggt
    31141 gcaatcattg aaaccccgct gggcaccatg cagggtcact acgaaatgat cgatgaaaat
    31201 ggcgtccctt tcagcatcga cattcccgta ttccgactcg ccgttcccac actcattcat
    31261 taaaagaata tggcgacata ccttattggc gacgttcatg gttgttacga tgaactgatc
    31321 gcattgctgc ataaagtaga atttacccct gggaaagata ccctctggct gacgggcgat
    31381 ctggtcgcgc gcggcccggg ttcgctggat gttctgcgct atgtgaaatc cttaggcgac
    31441 agcgtacgtc tggtgctggg caatcacgat ctgcatctgc tggcggtatt tgccgggatc
    31501 agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg aagcgccgga tgccgacgag
    31561 ctgcttaact ggctgcgtcg ccagcctttg ctgcaaatcg acgaagagaa aaagttggtg
    31621 atggcccacg cagggatcac gccgcagtgg gatctacaga ccgccaaaga gtgcgcgcgc
    31681 gatgtagaag cggtgctatc gagtgactcc tatcccttct ttctcgacgc tatgtacggc
    31741 gatatgccaa ataactggtc accggaattg cgggggctgg gaagactgcg ttttatcacc
    31801 aacgccttta cccgtatgcg tttttgcttc ccgaacggtc aactggatat gtacagcaaa
    31861 gaatcgccgg aagaggcccc tgccccactg aaaccgtggt ttgcgattcc tggccctgtc
    31921 gctgaagaat acagcatcgc ctttggtcac tgggcatcgc tggagggcaa aggtacgccg
    31981 gaaggtatat acgcgctgga taccggctgc tgctggggtg gtacattaac ctgcctgcgc
    32041 tgggaagata aacagtattt tgtccagccg tcgaaccggc ataaggattt gggcgaagcg
    32101 gcggcgtctt aaacacagcc tgatatagga aggccggata agacgcgacc ggcgtcgcat
    32161 ccggcactag ccgtaaattc tatacaaaat taccgccgct ccagaatctc aaagcaatag
    32221 ctgtgagagt tctgcgcatc agcatcgtgg aattcgctga ataccgattc ccagtcatcc
    32281 ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg cgtcagatac
    32341 agtttctgcg cttttggcag gaactgctca taaacgcgac cgccgccaat caccatgatt
    32401 tctggtacgt caccacacgc cgcgatggct tcatccaccg acttcaccca cgttacgcga
    32461 tcgtccgtac ccggttgact gctgaggata atatttttgc gtcctggcaa cggacgaccg
    32521 attgattccc aggtatggcg gcccataatc acgggtttat ttaaggtgtt gcgtttaaac
    32581 caggcgagat cggcaggcag gttccacggc atggcgtttt ccatgccgat aacgcgatct
    32641 accgctaacg ccgcaatcag actgatcatt gagatttccc gataaaaaaa attgtcgcca
    32701 ctatacgtaa agcgtaaacc gtcgtcgact ggtgcgagga tgatgttgag gaaaatttta
    32761 tattctgctg gcgagtccac gctctctccc tggactcgcc gcattacaat gaaacaaaaa
    32821 caaacagtta gctgtaaagt gtgatttatg tcactcttta ttaggatgag ggtttcgttt
    32881 ccggttcatc cgccatgttg ccggtatgtt taccttcttc ggttccctgc cagccatgtc
    32941 gttgaattaa tgacagatgt tcgcggtcct cggtaatgat ctcacttaac atcgcgctgg
    33001 tgcgtttata gaccgccgcg cgggctttgg tgtcgttctc aaccattgcc atctcttcca
    33061 ccatctgaat attaaagcgg cggaacacat cggcacgttc tcgcgcttca tacggcccca
    33121 gacctaaact ttccagtgcc agacgcccgg ttttcagcgc accttcgaag gtttcacgct
    33181 ccggcttttc aacgcctgcc tgacgcaaac gaatgtagtg gtcgacatcg cgggcgcggg
    33241 caataatctg caaatgcggg aagtgttctt tcaccatctc tgtcagttgc aggttggttt
    33301 gcggatcgtc gatggcgtta atcagcactt ccgctttcgc cgctcccgca gattccagta
    33361 aatccatccg cgtggcatcg ccataaaaca ctttcatacc aaatttacgc aaggtttcga
    33421 tatggtccgg atcgtgatcg agcaccacca ttttcacccc gctggagagc agtaaacgtc
    33481 cggtgatctg cccaaaacga ccgaatccgg caatgattac ccgcggctgt tcttcgtcga
    33541 tctcatcggc ttcacgcgcc tcttcagtag aagattgctc aaggcgattg aggatcacca
    33601 gcagaatcgg cgttgccgcc atcgacagcg ccaccgccag ggtcagcgat ttcgcccact
    33661 ccggctccag cacattcgcc atctgcgccg cgccaaatac cacaaaggca aactcactgc
    33721 cctgccctaa caacaccgca aaccaacgac gctgtttatt tggcacttgc aacggtcggg
    33781 caatcagcca cagcatggcg attttgatga tgaggaaacc gagcagcaaa atgacaatgc
    33841 gcaatgggtt ttcaagcagc gtgccaaagt ctatcgacat gccaacaccg atgaaaaaca
    33901 gccccaacag caaaccttta aagggttcga tatcgctctc cagcgcatga cggtattccg
    33961 agcttgccag cagtacgccc gccagaaacg cgcccatcgc catcgacaag ccgacctctt
    34021 ccagcagcaa accaaagcca aacacgagga ataacgccac ggcactaaac acttcccgca
    34081 agccagagcg ggctacaaaa cgcagcgccg gacgcgtgac atagcgcccc agcaatacca
    34141 ccagcaccag cgcacccgcc acttttaacg ccgagagagc aaatgcgccc atcgtcgtcg
    34201 aggcactgct cgttgccagt agcggaatca tcgccaccag cgggatcgcc gcgatatcct
    34261 ggaacagcag caccgcaaag gcactgcgac ccatttgcgt caccatcaga ttgcgttcat
    34321 tcatcgcctg catggcaatc gccgtagagg agagcgccag cgtcatgccg atcaattccg
    34381 cgacctgcca gcgcagccca agtaacatgc agaacagccc cagcaggccg ccgcaaatca
    34441 ccatctgcaa tgcgccaccg ccgaacactg ccgcacgcag cttccacagc ctttgtggat
    34501 cgagttcgag gccgataata aacagcatca gcaccacccc aatctcggca aagtgcagaa
    34561 tagattcggc atcggtcacc agtcgcagcc cccacgggcc aataatgcag ccggcgatca
    34621 ggtagccaag taccgatccc agaccaagac gtaccgcaat gggtacaatc agcgctgccg
    34681 aaccgagata aatcagcgcc tgaatcagcg tatggctatc catgatgggc ctcctgccat
    34741 tccagcagac gttgcttata gtgacgcgcc tgcccttcga gggtttcgtc gtcacaaata
    34801 aaggtgcagt gcatggcaaa cggtggcagc cagttcagcc cgcagtagat tgccgtcgcc
    34861 tgtagcggct gcgacagcac atcaaagccc ggatgcgcac caatttcaaa atggctttcc
    34921 ccgccgccgg tcgtcaccgc ccacagcaaa tgtttgccat gcagcgcctt gccgccgtga
    34981 ccgtaagccc agccgtgcga caaaacttta tcgatccaaa gtttaagaag cggaggaatg
    35041 ctgtaccact gcatcggatg ctgccagacg atcagatcgg cgcgagacag cgcctcctgc
    35101 tcggcggcaa tatcgatatt gaagtcagga tagagttgat aaagagagcg aatttcgacg
    35161 ccttccagcg tccttgcctg ttcaagcatc cgtttattcg catgggaatg atgcggatac
    35221 ggatgcgcat aaattataag aatcataggt agcccgcgtg attacgcctt tgttttccca
    35281 caaagtgtag tcatatattc acgcggctta tagtcaataa tattgattca gaaaagagag
    35341 aaatctgaac aatttacccc attctctgcc cgcggatcgt catattgctg gtttgcgcca
    35401 atgacatccc tcgatgtctc cggggcaaac gctacggaaa tcaacaagcc aaacagcgag
    35461 atacccgccc ccattagcat cgtgttactg ataccgtaat tattgataaa gatcggcagt
    35521 gcccaggtcg aaacaatggt gccaatacgg cttaaggaca taatcacgcc cacggcagag
    35581 gcgcggatat ctgtcgggaa gagttcatta ggatagagcc actgcaaatt acccggcccg
    35641 ccagagaaaa aggcatacac cgcaaaggcc atcactacca gccagatccc catatccggg
    35701 atcagcccca aaaccgccag ctccagcgtc atcatggcaa agctgccaat caacaatgga
    35761 cgccgtccgg cagtgtttaa ccacagcatc ggcggaatac agccgagcat aaagaacagg
    35821 ctaatcacca cgttccccag cgccgcgttt ttgccaacac ccaatcccaa caaaccaacg
    35881 atttgcgggc caaaggtgta aatggcgaac attgggatca cctggcaggt ccagatggcg
    35941 gcaacaaaca gaacaaaagg aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc
    36001 tgcggctgct cttcatcgaa agccaccggt tcgccaaaca gttttatcat catctcttcg
    36061 cactctttta ctcgcccttt gcgtaataac cagcggggag attcaggcag ttcgaatcga
    36121 ccaatcaaaa tcaacaaaca ggggatcgcc gcgctaccca gcatccagcg ccagccgcct
    36181 tccacatcat aaagccagta gccgaccaga tcggcgcagg tcgcgccgac ataccacatg
    36241 gcggcgataa agctgatgga aaatgcccgc tgacgggtac tggagaactc ggtgatcatt
    36301 gaggtggcga tgggataatc ggcaccgatg acaatgccga taagtacccg catcaccaac
    36361 agttcgacgg gggatgaaac aaacatcgtc gccaccgata tcacgccgat ggcgatgata
    36421 tcaatgagga acattttgcg ccgtccgact ttatcggaaa tatagccaaa cagcgatgtg
    36481 ccaacgaaca gcccggcgag cgttcccgcg cccaacaagc caatccagtc agcgtccagt
    36541 tttagcgccg gagtca
//
LOCUS       DAJTYD010000020        36530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTYD010000020 DAJTYD010000000
VERSION     DAJTYD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.020"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..896
                     /locus_tag="OO585_000907"
     CDS             <1..896
                     /locus_tag="OO585_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6628580.1"
                     /translation="DIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQ
                     IGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTY
                     PTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENL
                     TISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINP
                     TIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIR
                     NHRYTATMLQHIAEDRDGTNH"
     gene            complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO585_000908"
                     /pseudo
     CDS             complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO585_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO585_000909"
     CDS             complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO585_000909"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6628581.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO585_000910"
     CDS             complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO585_000910"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6628582.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKRTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO585_000911"
                     /pseudo
     CDS             complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO585_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO585_000912"
     CDS             complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO585_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6628583.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7802..8461
                     /gene="rpiA"
                     /locus_tag="OO585_000913"
     CDS             7802..8461
                     /gene="rpiA"
                     /locus_tag="OO585_000913"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6628584.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8716..9948
                     /gene="serA"
                     /locus_tag="OO585_000914"
     CDS             8716..9948
                     /gene="serA"
                     /locus_tag="OO585_000914"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6628585.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10177..10236
                     /gene="ibsC"
                     /locus_tag="OO585_000915"
     CDS             10177..10236
                     /gene="ibsC"
                     /locus_tag="OO585_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6628586.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10569..10628
                     /locus_tag="OO585_000916"
     CDS             10569..10628
                     /locus_tag="OO585_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6628587.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10729..11331)
                     /locus_tag="OO585_000917"
     CDS             complement(10729..11331)
                     /locus_tag="OO585_000917"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6628588.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11352..11535)
                     /gene="ssrS"
                     /locus_tag="OO585_000918"
     ncRNA           complement(11352..11535)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO585_000918"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO585_000919"
     CDS             complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO585_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6628589.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12074..12652
                     /gene="ygfB"
                     /locus_tag="OO585_000920"
     CDS             12074..12652
                     /gene="ygfB"
                     /locus_tag="OO585_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6628590.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12678..14003
                     /gene="pepP"
                     /locus_tag="OO585_000921"
     CDS             12678..14003
                     /gene="pepP"
                     /locus_tag="OO585_000921"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6628591.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14000..15178
                     /gene="ubiH"
                     /locus_tag="OO585_000922"
                     /gene_synonym="visB"
     CDS             14000..15178
                     /gene="ubiH"
                     /locus_tag="OO585_000922"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6628592.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15201..16403
                     /gene="ubiI"
                     /locus_tag="OO585_000923"
                     /gene_synonym="visC"
     CDS             15201..16403
                     /gene="ubiI"
                     /locus_tag="OO585_000923"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6628593.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16477..16560)
                     /locus_tag="OO585_000924"
     CDS             complement(16477..16560)
                     /locus_tag="OO585_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628594.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16850..17944
                     /gene="gcvT"
                     /locus_tag="OO585_000925"
     CDS             16850..17944
                     /gene="gcvT"
                     /locus_tag="OO585_000925"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6628595.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17968..18357
                     /gene="gcvH"
                     /locus_tag="OO585_000926"
     CDS             17968..18357
                     /gene="gcvH"
                     /locus_tag="OO585_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6628596.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18476..21349
                     /gene="gcvP"
                     /locus_tag="OO585_000927"
     CDS             18476..21349
                     /gene="gcvP"
                     /locus_tag="OO585_000927"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6628597.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYL"
     gene            21615..22358
                     /locus_tag="OO585_000928"
                     /pseudo
     CDS             21615..22358
                     /locus_tag="OO585_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO585_000929"
     CDS             complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO585_000929"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6628598.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23893..24204
                     /locus_tag="OO585_000930"
     CDS             23893..24204
                     /locus_tag="OO585_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6628599.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24368..25027
                     /locus_tag="OO585_000931"
     CDS             24368..25027
                     /locus_tag="OO585_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6628600.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO585_000932"
     CDS             complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO585_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6628601.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26446..26712
                     /gene="sdhE"
                     /locus_tag="OO585_000933"
     CDS             26446..26712
                     /gene="sdhE"
                     /locus_tag="OO585_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6628602.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26693..27100
                     /locus_tag="OO585_000934"
     CDS             26693..27100
                     /locus_tag="OO585_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6628603.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO585_000935"
     CDS             complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO585_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6628604.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27773..28669
                     /gene="xerD"
                     /locus_tag="OO585_000936"
     CDS             27773..28669
                     /gene="xerD"
                     /locus_tag="OO585_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6628605.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28694..29404
                     /gene="dsbC"
                     /locus_tag="OO585_000937"
     CDS             28694..29404
                     /gene="dsbC"
                     /locus_tag="OO585_000937"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6628606.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29410..31143
                     /gene="recJ"
                     /locus_tag="OO585_000938"
     CDS             29410..31143
                     /gene="recJ"
                     /locus_tag="OO585_000938"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6628607.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31234..32332
                     /gene="prfB"
                     /locus_tag="OO585_000939"
     CDS             join(31234..31308,31310..32332)
                     /gene="prfB"
                     /locus_tag="OO585_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6628608.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32342..33859
                     /gene="lysS"
                     /locus_tag="OO585_000940"
     CDS             32342..33859
                     /gene="lysS"
                     /locus_tag="OO585_000940"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6628609.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO585_000941"
     CDS             complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO585_000941"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6628610.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34505..34576
                     /locus_tag="OO585_000942"
     CDS             34505..34576
                     /locus_tag="OO585_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628611.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34573..34698)
                     /locus_tag="OO585_000943"
     CDS             complement(34573..34698)
                     /locus_tag="OO585_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628612.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO585_000944"
     CDS             complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO585_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6628613.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8796 a         9197 c         9768 g         8769 t
ORIGIN      
        1 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
       61 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
      121 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
      181 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
      241 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
      301 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
      361 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
      421 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
      481 tgactatcag tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag
      541 agcgtcaaga gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc
      601 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
      661 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
      721 tgacggtgat cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg
      781 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
      841 ataccgccac gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
      901 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
      961 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
     1021 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
     1081 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
     1141 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
     1201 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
     1261 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
     1321 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
     1381 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
     1441 ttgtcaggct ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccaccg
     1501 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
     1561 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
     1621 gaatacgcca tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg
     1681 gcacatggga ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac
     1741 ggcgacaatc tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg
     1801 gaagatcgac acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc
     1861 tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag
     1921 ccaggtcggc gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc
     1981 cgatgcttca atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc
     2041 cacttcgctc aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa
     2101 accggacgat gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc
     2161 gtcagcggcg gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc
     2221 ctcatgctgt tcgttagctc tgcgggcaat cgcggtgggt agggctttcg gcgaacccgc
     2281 cggggtaaag ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc
     2341 ggcggtcatc cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga
     2401 ccaacgaaat taggtttacg tttttcgagg aaagcgttca ttccttcctg gtaatcttcg
     2461 ctgtcataaa ccgcgcgtcg catcccctga atacgttcaa attcatcgga gttcatggtg
     2521 tgcgcttcgc ccagtacacg cagctcttct ttgataacag caatggctaa cggcgctttc
     2581 tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca
     2641 tggttgagga tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc
     2701 agctctttga caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta
     2761 tacgggacgc cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc
     2821 agatcggaac tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa
     2881 atgatcggtt tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag
     2941 gagagcggat cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact
     3001 ttggatccac tcggcgcgcg caaaatgata cagcgaattt ccggccggtt gagatcgctt
     3061 aacgcctgca taagatcatc aataaagact ttacttaagg cattaagttt tcggccatag
     3121 ttaaactcaa tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata
     3181 aaaattcctt taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt
     3241 gcgcggtgag agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa
     3301 atcttcattc gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc
     3361 caccgattgt tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa
     3421 ctcgatgata gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag
     3481 aacccgtggc tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat
     3541 atgccgggca atggcgacat tggtatggtt atcgccatcg tctttgttga taacgatcag
     3601 atcagccact tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat
     3661 ttgcaacgag ataaaacagt ccaccatgcg ggcgacttct gtttccgact gcccgacgcc
     3721 aaccgtttcg acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg
     3781 cgctcgctga ctggcaccgc ccagatgacc ggaggatggt accgggcgaa taaacgccgc
     3841 ttcggcacgc gccaggtcat tcatgcggat tttatccccg agaatgctac cgccagtgac
     3901 cgggctgctg ggatcgaccg caataaccgc gacctttaat ccctctcgaa tcaacaacat
     3961 gccaaaggcc tcaagaaagg ttcttttccc cgcgctgggg gtgccggtaa cgcccagtcg
     4021 cagggtgtta ccgcagtacg gcataatggc atcaagcagc tgcgtactta gtgcctgatg
     4081 acgcgggtga cggctttcca ccagcgtcat ggcctgggcg agtgtggcac gctcaccctg
     4141 acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca
     4201 gattcagtac gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca
     4261 cgccgcgctc ttgcaggaag gcgtaatcct gcggtggaat gacgccaccg gcgactacgc
     4321 agatatcttc gcgtccccat tttttcagcg cctcgaccag ttccgggatc agcgttttat
     4381 gaccggcagc cagtgaggat gcgcccacta cgtgaacgtc attttctact gccaggcggg
     4441 cgatctcttc aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat
     4501 aggcgctggc gatcactttc gagccacgat cgtgcccatc ctggcccatt ttggcgatca
     4561 gaatgcgcag gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat
     4621 cgaactcgga ggccgatttc tcagattgat gatagctttg cgcaatcacg ccggtaacac
     4681 actggcttgg caccagataa cggtcgaaag cggcttccag cgcatcggaa atttcaccca
     4741 gggtggcgcg aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg
     4801 cggcgtgagt cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa
     4861 tgcgttccag cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat
     4921 cggtttcgtc ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgtttgccct
     4981 ggtcgataag cgactgttcg cgcgctgagg cctattcgat cattcgtttt ggcagacctg
     5041 cttcgatcgc tttcgccata ccaccggctt cgtcgatctg ttggataata gctctggctt
     5101 gtttgacgat ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg
     5161 tgcggcagag ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg
     5221 agaaatcggt aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac
     5281 cgcccagcgt cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct
     5341 gttcagtcag gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcgggt
     5401 cctgtgcgcc aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg
     5461 cgacgttcat aaacagatcc atgccgatgc cgaagaagaa cgacaggcga ggagcgaagt
     5521 catcaatttt cagtccggca gagattgctg ctttgatgta ctcaatccca tcagcgagcg
     5581 taaatgctac ctgctgcacg cagttggcac ccgcttcacc catgtggtaa ccgctgatac
     5641 tgatggtatt aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc
     5701 gcattgacgg ttttggcggg taaatatagg tgttgcggca gaggtactct ttgaggatat
     5761 cgttctgaat ggtgccggtc agtttatcag gtgtaacacc ttgctcttcc gcggcgacga
     5821 tataaaacgc cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat
     5881 ccagcgggat ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg
     5941 ctttgccgac gtcgcccgcc acgcgcgggt tatcggagtc gtagcctcgg tgggtggcaa
     6001 ggtcaaacgc aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt
     6061 tggactcttt tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat
     6121 acatagtggc acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct
     6181 ccagattatc gagatcggct tcggtataca gcggcttgat ggtgatccct tccgcggttt
     6241 gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt
     6301 gccactcctg cacgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt
     6361 gcgcctacgg tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt
     6421 cgttttttgc cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa
     6481 gtgaggacaa cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag
     6541 gactttgtga ccataatcga gcaacgcatc agtgacttta cgcatcatgc ggctttcagg
     6601 agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc cctggcgtta agtcaatcag
     6661 ttcaccgctg gctagttctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc
     6721 ctggcgagca agttgcacga acgcttctga agaattaacg atatggcagg gcacgctgcc
     6781 tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa
     6841 ggcgaccaca ggtgctttca ataatgccga acgcgttacg ccgttaggga aatatttttc
     6901 ggcaaacggt tttgagctga cgaacagata gtcaagcgca ccgagcttat cgacaaggca
     6961 actcggcagc gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg
     7021 ttcctgagta cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa
     7081 tacaggagcc agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga
     7141 aagcagcagc ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc
     7201 cacctggcgc agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt
     7261 acgcaccaac agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc
     7321 tgattgtgta atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc
     7381 atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta
     7441 aatgcttttt cctgcactat gacacaattt tgtgtcaggt tgcatatacc gcagccgtcg
     7501 gcgggtaaca tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta
     7561 ttgcatttcg cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct
     7621 ctgttcgcac cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag
     7681 gggatgcccg ccattgcgcg atattttatg gatgagttaa ccacccggct tgccaacggg
     7741 gtctgaatcg ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat
     7801 catgacgcag gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc
     7861 cggcaccatt gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg
     7921 cacgatgaaa ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact
     7981 gaaaagcctc ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt
     8041 tgatggcgca gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct
     8101 gacccgtgaa aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc
     8161 caagcaggtt gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg
     8221 tagtgcagtg gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt
     8281 ggtgaccgat aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat
     8341 agcgatggaa aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa
     8401 ccgtggcgcg gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg
     8461 atctgacggg ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg
     8521 tcacgttttt acctggctat tgtcgattgc tctaaataaa tcctctaaac cagcatattc
     8581 aaccaagaaa tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc
     8641 cgcaatatta ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa
     8701 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
     8761 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
     8821 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
     8881 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaag
     8941 ctggtcgcta ttggctgttt ctgtatagga acaaaccagg ttgatctgga tgcggcggca
     9001 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
     9061 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
     9121 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
     9181 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
     9241 tatgtttact tttatgatat tgaaaataaa ctgccgctgg gcaacgccac tcaggtacag
     9301 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
     9361 tccaccaaaa atatgatggg cgcgaaagaa atttcactaa tgaagcccgg ctcgctgctg
     9421 attaatgctt cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
     9481 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
     9541 ccatttacct ctccactgtg tgaattcgac aacgtacttc tgacgccaca cattggcggt
     9601 tcgactcagg aagcgcagga gaatatcggc ctggaagttg cgggtaaatt gatcaagtat
     9661 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
     9721 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
     9781 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
     9841 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
     9901 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
     9961 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    10021 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    10081 cgttgtgttt atttaatggg gatttacgat ggcgggacag tatggggctg taacggtaaa
    10141 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    10201 attgtactgt tactgataag tttcggagct tattaacagt cagtctctgg ggaggagtca
    10261 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    10321 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    10381 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgaacac
    10441 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    10501 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    10561 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    10621 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    10681 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    10741 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    10801 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    10861 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt actcatcaaa
    10921 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    10981 cagttttggc tcatggattt tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    11041 caggaacagc aaattaccag cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    11101 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    11161 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    11221 ttgacccatt tcctgctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    11281 gcggatttct tgtcgggata atgtcagtgg gagttctggt agttgcgtca tggctggtgc
    11341 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    11401 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat gctcaccaac
    11461 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    11521 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    11581 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    11641 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtcttcgct
    11701 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    11761 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    11821 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    11881 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    11941 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    12001 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    12061 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    12121 tctgaaccaa caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    12181 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    12241 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    12301 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    12361 cagcgttttc gatcgggctg atgcattggc aggttgggtc aatcacttcc tgcttggtct
    12421 tggcgttacg caaccgaagc tggataaagt gaccggcgaa accggtgaag ctatcgacga
    12481 tctgcgtaac attgcgcaac tgggttacga cgaagacgaa gatcaggaag agcttgaaat
    12541 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    12601 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    12661 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    12721 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    12781 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    12841 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    12901 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    12961 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    13021 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    13081 atgcatatgc tgatgaaatc atgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    13141 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    13201 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    13261 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    13321 aaattcacca cgaatttaac cgccacggtg cgcgctatcc gtcctataac accattgtcg
    13381 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    13441 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    13501 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    13561 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    13621 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatt ctgaaaggtg
    13681 atgttgatga actgatcgct cagaatgccc atcgtccttt ctttatgcat ggccttagcc
    13741 actggttagg gctagatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    13801 tggaaccggg catggtactg accgtagagc cagggctgta tattgcgccg gatgcagaag
    13861 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    13921 ccggtaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    13981 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    14041 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    14101 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    14161 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggcgg attgcgcaac
    14221 tgccatcacc accgtgcatg tcagcgatcg tggtcacgct ggatttgtca ccctcgccgc
    14281 agaagattac caactggcgg cgctgggaca ggttgtcgaa ttgcacaatg tcgggcaacg
    14341 gctgtttgca ttgctgcgta aagcacctgg cgtaacgctg cattgccctg atcgcgtggc
    14401 taacgttgcc cgtactcaga gtcacgttga agtgacgctg gagagtggcg agacgctgac
    14461 gggccgcgtg ctggtagcag ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    14521 tgactggcag caggagcctt acgaacaact ggccgtgatt gccaacgttg cgacttccgt
    14581 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    14641 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    14701 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg gctggcgact
    14761 tggaaaaatt acccacgccg gtaaacgcag tgcttatccg ctggcgttaa cccgcgccgc
    14821 cagagctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    14881 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    14941 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    15001 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    15061 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    15121 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    15181 gcggttttaa ggaataaaca atgcaatgtg ttgatgtagc cattgttggt ggcggcatgg
    15241 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    15301 agcgcgtacc ggaacctctg gcggcggatg caccaccaca actgcgcgtt tcggctatca
    15361 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctctctcgta
    15421 gggccagttg ttatcacggt atggaagtgt gggacaaaga cagttttggt cacatttcgt
    15481 ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac tcagtgattc
    15541 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat cactctgttg gcccccgcag
    15601 aattacagca ggtcgcctgg ggagaaaatg aaactttcct gacgctgaaa gatggcagca
    15661 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    15721 cagatatttc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    15781 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    15841 ttttaccgct tagcgatccg catctttgct caattgtctg gtcactgtcg ccagaggaag
    15901 cgcagcggat gcagcaggca agtgaagatg aatttaatcg cgcgttaaat atcgcttttg
    15961 ataatcgcct gggcttatgc aaggttgaga gcgcgcgtca ggtgttccca ctgacggggc
    16021 gttatgcgcg ccagtttgcc gcgcaccgtc tggtgctagt gggcgacgcc gcgcatacca
    16081 ttcacccgct ggcggggcag ggagtaaatc tcggctttat ggatgctgca gagctgattg
    16141 ctgaactgaa acggttgcat cgtcagggga aagacatcgg gcagtacatt tacctgcgcc
    16201 ggtatgagcg tagccgcaag cacagtgcgg cgctgatgct ggctggtatg cagggattcc
    16261 gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    16321 tggccgatac gcttcctggc gttaaaccac aacttatccg ccaggcaatg ggattaaacg
    16381 atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat acccatcaca
    16441 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    16501 ctccgttttc gcattatatt ttctaatgtc attatttttt gatttagtgt tttttgacat
    16561 ttttttagct cttaatattg ccttattcaa attagatttc taatggcatc atctttatat
    16621 agaaactggt gtgttttttg gttttttatt ctgtcgcgat tttggcgttt tttaaccata
    16681 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    16741 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt ttcgtggtga
    16801 taatttcacc atgaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    16861 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    16921 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    16981 cggaatgttt gatgtgtcac atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    17041 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    17101 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gacctcatcg tctactactt
    17161 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    17221 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    17281 gattgccgtg caagggccga atgcgcaggc aaaagctgcc acactgttta atgacgccca
    17341 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat
    17401 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    17461 ggccgatttc tggcgtgcgc tggtggaagc gggtgttaag ccatgtggct tgggcgcgcg
    17521 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    17581 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccggcag atcgtgactt
    17641 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    17701 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    17761 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    17821 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    17881 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    17941 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    18001 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    18061 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    18121 gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa aagcggcgtc agacatttat
    18181 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc tccggaactg
    18241 gtgaacagcg aaccgtatgc aggtggctgg atctttaaaa tcaaagccag cgatgaaagc
    18301 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga cgagtaacgg
    18361 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    18421 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    18481 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    18541 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggcgca caatcgttaa acgcgctgac
    18601 cggccagatt gtaccgaaag atattcagct tgcgaccccg ccgcaggttg gcgcaccggc
    18661 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    18721 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    18781 gctggaaaat ccgggctggt ataccgcgta cactccgtat caacctgaag tctcccaggg
    18841 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    18901 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    18961 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccgatg tgcatccgca
    19021 aacgctggat gtggtccgta ctcgtgccga aacctttggt tttgaagtga ttgtcgatga
    19081 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    19141 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    19201 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    19261 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    19321 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    19381 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    19441 gcaacatatc cgccgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    19501 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    19561 ccgcattcac cgtttgaccg atatcctggc ggcaggcctg caacaaaaag gtctgaagct
    19621 gcgccatgcg cactatttcg acactctgtg tgtggaagtg gccgacaagg cgggcgtact
    19681 ggcgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg cggttgggat
    19741 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagcg tgctgctggg
    19801 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    19861 tattcagcct gcgatgctgc gcgacgacga aatcctcact catccggtgt ttaatcgcta
    19921 ccacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    19981 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    20041 gatcccaatc acctggccgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    20101 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    20161 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    20221 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    20281 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    20341 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    20401 gggcgataac ctctcctgta tcatggtgac ttatccctct acccacggcg tgtatgaaga
    20461 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    20521 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    20581 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    20641 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    20701 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    20761 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    20821 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    20881 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    20941 gaaagaagaa accagcatca gcgagctgga tattgccaag cgcctgatcg actacggttt
    21001 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    21061 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    21121 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    21181 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    21241 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    21301 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa tacctgtaat tcactgattc
    21361 gactattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    21421 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    21481 gcgcgaatat ttaccctcac cccggccctc tccctgaaag ggcgagggga aaagcgtgcc
    21541 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    21601 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    21661 caactgcatt actgttggcg aaagaagagt atacggtggc ggttaattat caacaaaacc
    21721 tccatgcggc gcaggaagtg gtgaacttaa tcacgcaagc cggtggcaag gcattcgtgc
    21781 tccaggcgga tatcagcgac gaaaatcagg tcatcgcaat gtttacagca atcgatcagc
    21841 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    21901 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    21961 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    22021 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa taggttgatt
    22081 acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg gaagtcgccg
    22141 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    22201 gcggcggcga gcttggacgc gtcgatcgcg ttaagtcgaa catccccatg cagcgtggtg
    22261 gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa gcctcttacg
    22321 tcacgggaag ttttatcaat ttggcgggcg ggaaataaaa cagggaagtt gtctgaccgg
    22381 atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg ttgctggcaa
    22441 tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg ccagtaccgt
    22501 cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg gtagtgaatg
    22561 acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca tcgtaagtca
    22621 ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga
    22681 tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg
    22741 gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact ggatcaatct
    22801 gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca aaaccagaga
    22861 tcgcatcgcc ggtgccgcct tcggccttca ctgcgttgat catgtaatag ctgaaaccaa
    22921 gataatcgca ggtgccttca cgcagtacat ccagatcgcc atcttccatt ttgatgttaa
    22981 atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt agctgcacat
    23041 cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg tccgggttac
    23101 aggagtaagg atagagcggc accatcgcca gcatacagcc gactttcatc tccgggttaa
    23161 tgcgacgcgc ggctttcact gctagggcgc tggcgacaaa ctggtgatgc agcacctggt
    23221 acatcgtctc ttccgggttg tcatgctcgg tatacaccac gccggagcag cagtaaccaa
    23281 acagcggcgc acgccagtta cgctggttgt ttatttcgtt gaaggtcatc cagtatttga
    23341 ctttatgctt gtagcgttcg aataccactt ccgcgaaacg gacaaagaaa tcaacgactt
    23401 tacggttggt ccagctaccg tattgctgca ccagatgcag cggtatttca aagtgggaga
    23461 gggttatcac cggttcgatg ttgtatttca gcagttcatc gaacagagag tcgtaaaact
    23521 tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc gtccaggcga
    23581 tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct tccttgtagt
    23641 gaccataaaa atcaacggct tcatggtttg gatagtattt tcctggcaag acttctttgg
    23701 tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg ctcggtcctt
    23761 tgccgccttt gttccagccg ccttcgacct gatgagcggc aacggcgcca ccccataaga
    23821 aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc aattcttaca
    23881 ggaaatagta ccatgcagcc aaacgacatc actttttctc aacgtttcca ggatgacatt
    23941 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    24001 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    24061 gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac atgcagagca ggaaaatatg
    24121 accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac acagttttat
    24181 gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttctcattt
    24241 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    24301 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctccgga
    24361 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    24421 caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgggctgg tgttgctact
    24481 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagctaca gcctctatgg
    24541 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    24601 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    24661 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    24721 tatctggagc ctggcattgc tggggattct gtttaaactg accatcgcgc accgattcaa
    24781 aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg taatttatga
    24841 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    24901 ttcgctcgga gtgattttct atgtctgcaa acgcattccc tacaatcatg ccatctggca
    24961 cggcttcgtg ctcggcggca gtatgtgcca ctttctggcg atctatttgt atattgggca
    25021 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg
    25081 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    25141 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    25201 atgcctgatg cgatacagag atttactctt cgagtgaata cggcagcggc tcgatatgca
    25261 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    25321 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    25381 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    25441 cgctacctgc cagcaaccag agcgcacgtt tattggcacc acggaatttt gctcgcgcca
    25501 ccatctcttg tccggtataa cagcctttct taaaactgat accgcccagc gcctggagat
    25561 tggtcgcctg tgggataaac tgcccgctgt tcgcggcatc aatcaccggg aaacccgctt
    25621 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    25681 cggtcagcat attggcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    25741 caaaccatag caaagtggtc gcgccttctt taactacctg tttttctctc gaaggtagtt
    25801 cactaaagag attggcgagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    25861 gctcgtcgtc tgccgcgatg gtcactttag agaacaccgc atattttttc agttcagtca
    25921 gctgcggttc acgcacgctg cgccgttcaa tccacgcaaa gccatcgccg tcgcggaaca
    25981 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    26041 tttctgtcat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    26101 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    26161 cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    26221 cttaacggcg tattaataac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    26281 tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagctctt gaattgttgt
    26341 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    26401 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    26461 aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    26521 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    26581 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    26641 gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga acgtggtcct
    26701 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    26761 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    26821 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggcgta ttaatgctcg
    26881 ccagggggaa attcgattgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    26941 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    27001 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagctg aatggcggga
    27061 tttacggcgg attttgttgc aacaagagac gcaaagataa atatgacggc aaagcatatg
    27121 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    27181 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    27241 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    27301 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    27361 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaacaa
    27421 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    27481 tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    27541 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    27601 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    27661 taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga atcgctttag
    27721 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    27781 ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    27841 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    27901 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    27961 agagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    28021 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    28081 gaaattgcca cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    28141 ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    28201 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctacg
    28261 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttaggtga
    28321 agaggccgtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgttgaatgg
    28381 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    28441 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    28501 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    28561 ggtgcagatg ctactgggcc acagcgatct ctccaccacg caaatttata cgcatgtcgc
    28621 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    28681 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    28741 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcattaaaag
    28801 cagcgatatt cagccagcac ccgtggctgg catgaagact gtgctgacta acagcggtgt
    28861 gttgtacatc accgatgacg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    28921 cgcggctccg gtcaatgtca ccaataaaat gctgctaaag cagttgaatg cgcttgaaaa
    28981 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    29041 tacctgtggg tactgccaca aactgcatga gcaaatggca gactacaacg cgctggggat
    29101 caccgtgcgt tatcttgctt tcccgcgcca ggggctggag agccaggctg agcaacaaat
    29161 gaaagccatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggctggtaa
    29221 agctgccact ccggcaagtt gcgatatcga tattgccgat cattacgcac ttggcgtcca
    29281 gcttggcgtt agcggcactc cggcaattgt gctgagcaat ggcacacttg ttccgggtta
    29341 ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    29401 ttaattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    29461 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    29521 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    29581 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    29641 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    29701 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    29761 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    29821 aacggtattt cctcccatgc gggggttgaa cacgctcgct cgttgggcat tccggttatt
    29881 gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat cattaaccct
    29941 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    30001 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    30061 gcaattccta acctggcaga actgctggat ctggtcgcgc tggggacagt ggcggacgtc
    30121 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    30181 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaaccgtga tgcacaaaaa
    30241 ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg
    30301 gacgatatgt ccgtcggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    30361 ctggcaaatg aactcgatgc gctaaatcag acgcgaaaag agatcgaaca aggaatgcag
    30421 attgaagcct tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct acccggcggg
    30481 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgcatc
    30541 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg tacgctgaaa
    30601 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    30661 ctctaccccg gtatgatgct gaagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    30721 gaagaggata aattcgaact ctttcaacaa cggtttggcg agctggttac cgagtggctg
    30781 gacccttcgc tattgcaagg cgaagtggtg tcagacggcc cgttaagccc ggccgaaatg
    30841 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    30901 ccgctgtttg atggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    30961 aaagtcatgg tcgaaccggt cggcggcggt ccgctgctgg atggtattgc ttttaatgtc
    31021 gataccgccc tctggccgga taacggcgtg cgcgaagtgc aactggctta caagctcgat
    31081 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    31141 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    31201 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    31261 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    31321 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    31381 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    31441 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    31501 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    31561 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta
    31621 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    31681 gcgtatgtat ttgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    31741 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    31801 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaagagcc cgtttgactc
    31861 cggcggtcgt cgccacacgt cgttcagctc cgcgtttgtt tatccggaag ttgatgatga
    31921 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    31981 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    32041 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aataaagacc aggccatgaa
    32101 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgctg agaaacaggc
    32161 gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt atgtccttga
    32221 tgactcccgc attaaagatc tgcgcaccgg ggttgaaacc cgcaatacgc aggccgtgct
    32281 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    32341 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    32401 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    32461 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    32521 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    32581 gggtaaagcc tcttttgtta ccctgcagga cgttggcggt cgtattcagc tgtacgttgc
    32641 ccgtgacgat cttccggaag gcgtttacaa cgagcagttc aaaaaatggg atctcggcga
    32701 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    32761 caccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggacaaat tccacggctt
    32821 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    32881 ccgcaacacc tttaaagtgc gttcgcagat cctctctggc attcgccagt tcatggtaaa
    32941 tcgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    33001 gcgtccgttt atcacccatc ataacgcgct ggatcttgac atgtacctgc gtatcgcgcc
    33061 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    33121 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    33181 ctatatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    33241 tctggcacag gatattctcg gtaagacgga agtcacctac ggcgacgtga cgctggactt
    33301 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    33361 cgacatggcg gatctggaca acttcgactc tgcaaaagcg attgctgaat ctatcggcat
    33421 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    33481 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    33541 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    33601 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcagcgctt
    33661 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgacgaaga
    33721 ttacgtcacc gcactggaac acggcttacc gccgacagca ggtctgggaa ttggtatcga
    33781 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    33841 gatgcgtcca gtaaaataag cattacgtta tgctaataac cccggcaaat gtcggggttt
    33901 tttaattgtg ctgcgcgaaa gcagacaaca attttcttgc ttcactattg gctgcctgca
    33961 ttaccatcca cggactgaac gcccacggcg tggcatcaat accgtgtaat acatctgcta
    34021 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctgttggtgc
    34081 gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg gtggcgcgat
    34141 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    34201 aacggcggat cactgcgtct tcgttgcttt ctcccagttg tgggtgccca caaaccgagt
    34261 tagtccacac gccaggccat gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    34321 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    34381 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    34441 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    34501 tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    34561 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    34621 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac tgaatatagc
    34681 gcgcattaaa agatttatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    34741 gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc ttcaccaggt
    34801 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    34861 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    34921 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    34981 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    35041 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    35101 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    35161 tatgaacgcg cgtcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    35221 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg cgaataatat
    35281 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    35341 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    35401 gcataccaaa cgacatcggg gtgacaatcg caaaccacaa ggcatcatgt agcccggata
    35461 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    35521 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    35581 caaaggagat acctgaataa acgggattac catattgcgg attcccttta cctccggcgg
    35641 cccagtcaat acccacctga atgatgctaa gtccgacaga agtaataacc acaccggtaa
    35701 ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat gtggtgataa
    35761 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc aatatccggg ttcatcccaa
    35821 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    35881 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    35941 ccgagctaat gagcatcgca atagcttctt ttgagaggcc cagtcggtca ccaatcataa
    36001 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    36061 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    36121 agagaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa
    36181 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    36241 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg gttaaaccac
    36301 gaaaatgaga agagaacctg acaatggaat tatctgtaat tttctcatat tgatatttca
    36361 ttaatcattc tggcatgtcg tactctcgca tttaatcgtt tttatctgga tagcactctt
    36421 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    36481 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt
//
LOCUS       DAJTYD010000021        36305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTYD010000021 DAJTYD010000000
VERSION     DAJTYD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.021"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..709
                     /locus_tag="OO585_000945"
                     /pseudo
     CDS             1..709
                     /locus_tag="OO585_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            701..1273
                     /locus_tag="OO585_000946"
     CDS             701..1273
                     /locus_tag="OO585_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6628614.1"
                     /translation="MPEDDRIKAINEIRMAIHQVSPFREEPVDCVLWVKNSQLMPNDY
                     NPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRL
                     KGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGM
                     DSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1274..2423)
                     /gene="ybdL"
                     /locus_tag="OO585_000947"
                     /pseudo
     CDS             complement(1274..2423)
                     /gene="ybdL"
                     /locus_tag="OO585_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            2532..3620
                     /gene="hcxA"
                     /locus_tag="OO585_000948"
     CDS             2532..3620
                     /gene="hcxA"
                     /locus_tag="OO585_000948"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6628615.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3630..3827)
                     /locus_tag="OO585_000949"
     CDS             complement(3630..3827)
                     /locus_tag="OO585_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6628616.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4009..6114)
                     /gene="cstA"
                     /locus_tag="OO585_000950"
     CDS             complement(4009..6114)
                     /gene="cstA"
                     /locus_tag="OO585_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6628617.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6295..6708)
                     /gene="entH"
                     /locus_tag="OO585_000951"
     CDS             complement(6295..6708)
                     /gene="entH"
                     /locus_tag="OO585_000951"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6628618.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6711..7457)
                     /gene="entA"
                     /locus_tag="OO585_000952"
     CDS             complement(6711..7457)
                     /gene="entA"
                     /locus_tag="OO585_000952"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6628619.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(7457..8314)
                     /gene="entB"
                     /locus_tag="OO585_000953"
     CDS             complement(7457..8314)
                     /gene="entB"
                     /locus_tag="OO585_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6628620.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8328..9938)
                     /gene="entE"
                     /locus_tag="OO585_000954"
     CDS             complement(8328..9938)
                     /gene="entE"
                     /locus_tag="OO585_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6628621.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATASPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(9948..11123)
                     /gene="entC"
                     /locus_tag="OO585_000955"
     CDS             complement(9948..11123)
                     /gene="entC"
                     /locus_tag="OO585_000955"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6628622.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11498..12454
                     /gene="fepB"
                     /locus_tag="OO585_000956"
     CDS             11498..12454
                     /gene="fepB"
                     /locus_tag="OO585_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6628623.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12458..13708)
                     /gene="entS"
                     /locus_tag="OO585_000957"
     CDS             complement(12458..13708)
                     /gene="entS"
                     /locus_tag="OO585_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6628624.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            13819..14823
                     /gene="fepD"
                     /locus_tag="OO585_000958"
     CDS             13819..14823
                     /gene="fepD"
                     /locus_tag="OO585_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6628625.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            14820..15812
                     /gene="fepG"
                     /locus_tag="OO585_000959"
     CDS             14820..15812
                     /gene="fepG"
                     /locus_tag="OO585_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6628626.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            15809..16624
                     /gene="fepC"
                     /locus_tag="OO585_000960"
     CDS             15809..16624
                     /gene="fepC"
                     /locus_tag="OO585_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6628627.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(16621..17754)
                     /gene="fepE"
                     /locus_tag="OO585_000961"
     CDS             complement(16621..17754)
                     /gene="fepE"
                     /locus_tag="OO585_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6628628.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(17969..21850)
                     /gene="entF"
                     /locus_tag="OO585_000962"
     CDS             complement(17969..21850)
                     /gene="entF"
                     /locus_tag="OO585_000962"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6628629.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(21847..22065)
                     /gene="ybdZ"
                     /locus_tag="OO585_000963"
     CDS             complement(21847..22065)
                     /gene="ybdZ"
                     /locus_tag="OO585_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6628630.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22068..23270)
                     /gene="fes"
                     /locus_tag="OO585_000964"
     CDS             complement(22068..23270)
                     /gene="fes"
                     /locus_tag="OO585_000964"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6628631.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWREPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            23513..25753
                     /gene="fepA"
                     /locus_tag="OO585_000965"
     CDS             23513..25753
                     /gene="fepA"
                     /locus_tag="OO585_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6628632.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            25928..26548
                     /gene="entD"
                     /locus_tag="OO585_000966"
     CDS             25928..26548
                     /gene="entD"
                     /locus_tag="OO585_000966"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6628633.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(26670..26822)
                     /gene="hokE"
                     /locus_tag="OO585_000967"
     CDS             complement(26670..26822)
                     /gene="hokE"
                     /locus_tag="OO585_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6628634.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            27276..28394
                     /locus_tag="OO585_000968"
     CDS             27276..28394
                     /locus_tag="OO585_000968"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6628635.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            28460..28708
                     /locus_tag="OO585_000969"
     CDS             28460..28708
                     /locus_tag="OO585_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6628636.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPTPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            28773..29141
                     /locus_tag="OO585_000970"
     CDS             28773..29141
                     /locus_tag="OO585_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6628637.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            29235..29888
                     /gene="nfsB"
                     /locus_tag="OO585_000971"
     CDS             29235..29888
                     /gene="nfsB"
                     /locus_tag="OO585_000971"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6628638.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            29996..31243
                     /gene="ybdG"
                     /locus_tag="OO585_000972"
     CDS             29996..31243
                     /gene="ybdG"
                     /locus_tag="OO585_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6628639.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(31311..32687)
                     /gene="pheP"
                     /locus_tag="OO585_000973"
     CDS             complement(31311..32687)
                     /gene="pheP"
                     /locus_tag="OO585_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6628640.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(32789..35916)
                     /gene="cusA"
                     /locus_tag="OO585_000974"
                     /pseudo
     CDS             complement(32789..35916)
                     /gene="cusA"
                     /locus_tag="OO585_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(35928..>36305)
                     /gene="cusB"
                     /locus_tag="OO585_000975"
     CDS             complement(35928..>36305)
                     /gene="cusB"
                     /locus_tag="OO585_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6628641.1"
                     /translation="LLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLL
                     IPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFL
                     IDSEANISGALERMRSESATHAH"
BASE COUNT         8681 a        10174 c         9308 g         8142 t
ORIGIN      
        1 gtacaccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc
       61 cctttcacaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca
      121 gcccccgcaa gatgcgataa ccgatcctga ctttttctgc ttttaccagc caggcatgac
      181 cttcgaacaa tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat
      241 gatgatcggc atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa
      301 taaacaacgt tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg
      361 gtacatttac cccatttacg actggaaagt ggctgatatc tggacctggt atgcaaatca
      421 tcaaagtctc tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca
      481 tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt
      541 tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg
      601 catttacgcc ggacatgaca atcatttcta tgggcatcgg aaaatcctca agccagaaca
      661 tttagactgg caagaatatg cattattgct gctcaatagc atgccggaag atgatcgcat
      721 caaagcgatt aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc
      781 agtggattgc gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa
      841 caacgtggca ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac
      901 ccaacccatt gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca
      961 ccgccatgaa attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc
     1021 ggtgacctgt ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg
     1081 cgctcgtgga cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt
     1141 gggatgggac gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg
     1201 cctgaagcaa attaacggcc tgcaagagtt gtttgccgac cgtcaatatt cccgcgcctg
     1261 gacggttaaa tagctaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct
     1321 tcttggcaaa acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca
     1381 gcggaatcgc cgccacgccg tgctcccgcg tcagccactg gcaaaactca acatcatcca
     1441 gggtagaaac ctcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct
     1501 ccagccggct ttcatttaaa gcattcacca gaatatcgcg cttctggcga taaaagtccg
     1561 gtaacgcaag ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg
     1621 gggtattcac cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg
     1681 ctggcgcaac acaataaccc actttccagc cggtcatatg atagatcttg ccaaatgaag
     1741 aaaccgccac tgcccgctca cgcagctgcg gatgcgccag cacactggca tggccctgtt
     1801 gtgaaaagtt gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga
     1861 tcgcctgcca caaagcggcg aaatcagcct gctgccagac agttgcactg gggttatgcg
     1921 gagtgttgag gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt
     1981 caaggtggtt gcagtgccat acgcttcact attcccccag aaagcgcgat ggcgggggcg
     2041 taactgtcat agctgggatc aaaacaaatc acttcatcgc cattgcgcac cagtgcggta
     2101 atcgccgcgt ataacgcttc cgtcgcccct gccgttacgg tgatatcgct atcggcatct
     2161 ggttgatagc catacaaacg ttccgttttc tgagcaatcg cctcgcgcaa ggcctgcacg
     2221 ccggtcatgg gcgcgtattg gtttgccccc tgggcaacgt ggtacgccag ccgctcctgt
     2281 aaatagcgcg gaccatcaaa atcaggaaag ccttgcgaca ggttaatcgc ctggtgttgc
     2341 tgcgccagcg cgctcatctg ggtgaaaata gtggtgccaa gttgtggaag tttgctttgt
     2401 ggaatcagag ggttatttgt catttttatt gtacctgacg aagttgcgtg ttgaaggcac
     2461 tataacacga tgctagtatt tggcaatcaa gacgtttaga tgtctaaata aaacaataag
     2521 gacaacacaa catgcctcac aatcctatcc gcgtggtcgt cggcccggct aactactttt
     2581 cacatccaag aagtttcaat cacctgaacg attttttcac tgatgaacaa ctttctcgcg
     2641 cggtgtggat ctacggcaaa cgcgccattg ctgcggcgca aaccaaactt ccgccagcgt
     2701 ttggacttcc aggggcaaag catattttgt ttcgtggtca ttgcagcgaa agcgatgtac
     2761 agcaactggc ggctgagtcc ggtgacgatc gcagcgtggt gattggcgtc ggcggcggcg
     2821 cactgctcga caccgcgaaa gccctcgccc gccgtctcgg tctgccattt gttgccgttc
     2881 cgacgatcgc cgctacctgc gccgcctgga caccgctctc cgtctggtat aacgatgccg
     2941 gacaggcgct gcattatgag attttcgacg acgccaattt tatggtgctg gtggaaccgg
     3001 agattatcct caatgcaccg caacaatatc tgctggcggg gatcggtgac acgctggcga
     3061 aatggtatga agcggtggtg ctggctccgc aaccagaaac gttgccgcta accgtgcggc
     3121 tggggatcaa taatgcgcag gccattcgcg acgtcttgtt aaacagtagc gaacaggcac
     3181 ttgccgatca gcaaaatcag cagttaacgc aatcattttg cgatgtggtg gatgctatta
     3241 ttgctggtgg tgggatggtc ggtggtctgg gcgatcgttt tacgcgtgtg gcggcggctc
     3301 atgccgtgca taacggtctg actgtcctgc cgcaaaccga gaagtttctt cacggcacca
     3361 aagtcgctta cggaattctg gtgcaaagcg ccttgctggg tcaggatgat gtgctggcgc
     3421 aattaaccgg ggcgtatcag cgttttcatc tgccgactac gctggcggag ctggaagtgg
     3481 atatcaataa tcaggcggag atcgacaaag tgattgccca caccttgcgc ccggtggagt
     3541 ccattcatta cctgccagtc acgctgacac cagatacgtt gcgtgcagcg ttcgaaaaag
     3601 tggaatcgtt taaagcctga cgtgcaaatt tagcagcagc gcgcgccgcc ttttccaccg
     3661 tagcgcgcgt cctgccgctc acggaaaaac tcctcgtagg tcatcggcgt ttgatcggga
     3721 tggttaaccc gcatatgttc gacatagttg tcgtaatcag gcataccaat catcagcttc
     3781 gccgcctgac ctaaatattt tccggctttt gccagtgaat caaacatctt ggcctccgct
     3841 aaccatgttg ctgaaagagt gccggatgcg gcgtgaacgc tttatccggc ctacaaatgc
     3901 tatcaacgca ctcggtcagt cccctcgccc catcggggag agggttaggg tgagggggaa
     3961 ccctcggcac tatgccaaca ttcgccaaca tcccccctca ctctgacttt agtgcgcgcc
     4021 tttcgcctgc gctacgatct cctcgacatt ttccggcatc ggctcatacg gcgtttcttt
     4081 cgccgttggc ttcggatctt tcaatgccgc aagtgccgtc ttaatcgaga acagtgccag
     4141 aaccaccacg accaccataa agaagatggt taacccggca tccagacggt tgttgaacac
     4201 cagttgcgcc agctgtgact cagtatactg cgacggaata ttgccgctgt cgatcattgc
     4261 ctggaacttg ttagcaatgg ccaggaagcc gactttcgca tccgggctaa acgctttctg
     4321 ccagccagcg gtcagggtac aaatcagcag ccaggccgtt ggtaccagcg ccacccaggc
     4381 gtaacgttga cgtttcatct tgaacaacac cacggcacag agcatcagcg ccatccctgc
     4441 cagcatctgg ttggcaatac caaacagcgg ccacagagtg ttaataccgc ccagcggatc
     4501 gaccacgccc tgatggagga agtagcccca cgccagtacg cacagcgccg ttgccagcag
     4561 gttagcaggc agtgaatcgg tccgcttcag gccaggagac accacgccca gcagatcctg
     4621 caacataaag cgcgcagcac gcgtacccgc atctaccgcc gtcagaataa acagagcttc
     4681 aaatagaatg gcgaagtgat accagaacgc cacatccatc ataccaccca gcgcaccgtg
     4741 caggatgtaa gccatcccca ccgccaacgt cggcgcaccg cccgcacggg aaatgatcga
     4801 ctgttcaccc acttcgctgg caatctggtt gagcgtatct ggtgtaatgc taaagcccca
     4861 gctactcacc acctgcgcgg cagaagcgac cacatccgcc gtccctgctg gagccagcac
     4921 cgccattggg ctgttcatgg caaagtaaac gcccggatcg atgatacagg cggaaaccag
     4981 cgccataatc gccacgaagg attccattaa cattccaccg tagccgataa aacaagcctg
     5041 cccttcgttc gccagcatct tcggcgtggt gccggaagag atcagcgcat ggaagccaga
     5101 caccgcgcca caggcgatgg tgataaacag gaacgggaac aggttaccgg tccataccgg
     5161 gccagtgcca tcgacaaatt tggtcagcgc aggcatggtc agcgtcgggc gcataatcaa
     5221 aatgcctacc gccagaccga cgatcgtccc gattttcagg aaggtagaga ggtagtcacg
     5281 cggggccagc agcaaccaca ccggcagcac cgccgccaca aaaccgtaac ccaccagcat
     5341 ccaggtcagc tgcacgccgg taaagtcaaa gtacggtgcc caggtcggac tttctgccac
     5401 ccagccgcca gagataatgg cgaaaatcag gaataccaga ccgatgaccg acacttcacc
     5461 aatacgcccc ggacgcagat agcgcaggta gatccccata aacagcgcca gcggaatggt
     5521 gaacgcaacg gtatacgttc cccacgggct atgagtcagg gctttcacca cgatcatcgc
     5581 cagtactgca aggataatga ccatgatcat aaagcaggcc accagcgcta tcaccccggc
     5641 ggttggcccc atctcttctt tgaccagctc acccagcgat cgaccgtcac ggcgcgtaga
     5701 aacaaacagc accatgaaat cctgcaccgc accggcgaga accaccccgg cgagtagcca
     5761 gatcatcccc ggcaggtagc ccatttgcgc tgccagcacc ggcccaacca acggacctgc
     5821 tccggcgatg gccgcaaaat ggtgaccgaa cagcactttc ttgtccgtcg gcacatagtc
     5881 cagcccgtcg ttatggcgca ccgctggcgt catacgcttc gggtcaaccg ccagcacatt
     5941 tttggcgata tacaacccat aaaaacggta agcgatcaga tagatacaga ccgacgccac
     6001 cacaatccac agcgcgttga tctgttcccc acgatttaaa gcaatgtatc ccagagcaaa
     6061 tgctcccatt acagagagca ctgtccagac gaggtatttc cctgatttgt tcatagttgt
     6121 tatccgtgtg cgtgtccata gagatgttac attttgtttc tataacatct ttgtacgatt
     6181 taacaacacg gtaactatgt aaatcggagt tgaaaccaat atttaactta acaatgttaa
     6241 ctcgatcact ccgttaaccg agttttcctg caatctcatt taattctgtc gggctcatcc
     6301 caaaactgcc gtacccagcc gacaagtgca gcaacgccgc ccctgttcat cgaaaacgac
     6361 gatttcccag ctctgatttt gccgcccaag atgcagcggc tggcagacgc cgcgtacctt
     6421 gccttcagac accgggcgat gatgggtggc gttaagttcc gtgcccacca cacactgccc
     6481 gtcacgggtc atcataaatc cggccatcga tcccagcgtt tcagccagcg ccgccgacgc
     6541 gccgccatgc agcaggccaa acggctgatg agtacgggta tcaaccggca tttcggcttc
     6601 cagcacatca tctcccagac gggtatacac aattcccaga tgcgccacca ttgtgttatc
     6661 gctggtggcg ttcagttcgt cgagcgttaa atggcgtttc cagatcatgc ttatgccccc
     6721 agcgttgagc cgccatcgac cacaatatcc tgtagggtga tatggctggc gaggtcagag
     6781 gcgaggaaca aaatcgtgtt ggcgatctct tgtggacggg cgattttccc cagcggaatg
     6841 ccgagtttaa actgctcgcc aaagccgcga atacgctgtt cttcggcgtc atcgctcacc
     6901 cacagcgtgc gttgcatatc ggtgtcggtg gagccaggcg aaaccacatt acagcgcacg
     6961 ccgctacccg ccagttccag cccgacgctc aacgccaggc ttttcagcgc cgctttcgat
     7021 gcgccataag cactcatacc aatacgcggc gtgtgtgcgg cgtcggacgc cacagtgaca
     7081 atcgccccgc cccgctgacg gcgaaactgg ttcatggttt gctggaacag gttaaacgca
     7141 ccgccgacgt taaccgcaaa agtctgctgc cagtcctctt tactgagctg atcggtcgcg
     7201 cccatgcgca aaattcccgc cgcattgatc agcacgtcca gtcgctccgt ttcagctaac
     7261 agtcgctgac atacctgcgc cacctgcccg gcgtcggcaa catccattac ttcggtcgca
     7321 aagggatatt gctcctgagt gaacgcttga tcaaaacctg taactttcgc tcccgcctca
     7381 acaaacgcca gcgccgtggc gtagccgata cctttacctg cgccggttac ccagacattt
     7441 ttaccgctga aatccattat ttaacctcgc gggagagtag cttccaccag gcgtcgatgg
     7501 tcgggttttt cgccagcatg acaaagtcga tatcaccatg cactttgcgc cagcgcgccg
     7561 ccagcgccat catgcgcacc gaatccagac cgtagtcgat caggttgtcg tcatcgaacg
     7621 gttcatcgga ctcgtccagc aacggcagga taacctcacg cagcgccgct ttgctggcgg
     7681 ggacaggtgc tggcagtaat tcttcagtca tcaccacccg gccagaccgc ccggccacat
     7741 atttcagcga catcaaatgc tcgtcacggc tgaaatcggc cagcgcatcc gccaccataa
     7801 acggtttaat atcgcgcata aatgcgtcgg ttgcggtggt catacagcca atgtgggcat
     7861 ataccccggt aataatcagc tggttacgtc cactctcttt gagcatttgc tccagcggag
     7921 aacgatgaaa cgcgctgtag cgccacttca ccagcaccgt gtcgtcggca tctggcgtca
     7981 ggcgatccac caccttttgc tgttccggcg aacgggtcag gcccggcccc cacatatcat
     8041 tcaacagcgc ccgatcttca tcgctctgct ctttcggctg ggcggtgtaa taaactggga
     8101 tattgtgctg tttgcagtag tcgcgcagcg cagcaatatt cgcgatcacc tgctccatca
     8161 tcgggcagtt ctcgccccag aagctgacaa aatagtcctg catatcatgg attaacaacg
     8221 cggcacgttg cagttcaaag gcccagtcaa ctttattctg cggaatatcg tgagactccg
     8281 gcagtgcgta agcctgtaat tttggaatag ccatcgtgtt ctctccttca ggctgatgcg
     8341 cgtgacgcca gccactgact taattgtttt ttatcgactt tcccgaccgc cgtcagcgga
     8401 agtgaatcta cacactccac gcgatccggt aatttaaatt cggcaatacc ctgttcacgc
     8461 aggaaacgac gcacctgcac cgcgcgcagc ggctctttta ccaccagata agcacagctt
     8521 ttttcgccca tcagctcatc ttccatgctc accagtgcgg catagatcac cgccggatgg
     8581 cgcagcagca ggttttcgat ctcttcggca gcgatcttct cgccgccacg gttgatctga
     8641 tctttctcgc gcccctgcac ggtgatgtaa ccctctggat caatagagat cagatcgccg
     8701 gaacagtaaa aaccgttggc atcaaaggcg ctggcattgt gctgcggact tttgtaataa
     8761 ccgcggaagg tgtacggccc gcgcgtcatc aggcgtccga cttccccttg cggcagtgga
     8821 tttccttcgg catcggcaac ccacacttca tcgtccggac acattgggta accctgggta
     8881 tggataattt tctccgcgct atcatcaaga cgggtgtagt tcaccagccc ttccgccatg
     8941 ccaaacacct gctgcaactg gcagccaatc tcagcgggaa tacgcgccgc aagggtggca
     9001 gaaagacgtg cgccgccgac ctgtaacagt ttcagcgagg caagctgcgc ccggctttcg
     9061 ccttcagcca gcgcctgcaa ccacaggctg actgccggcg gcaccagcgc ggtgacgttc
     9121 acctgatgtt tttcaatcaa tgggaagcaa agcgtagcgc tgggatcggc cgccagaaca
     9181 accgttcctc cggcaagaaa gacgcccagc gatcctggcg aactcatggc gtagttatga
     9241 gccgccggga tcgcgcacag gtagcgcgtc tgttgtgtga actgacaaat ctcgacgcta
     9301 cgacgcacgc tgtagtagta gtcgttatga gtgcgcggga tcagcttcgg tgtaccggtg
     9361 gtgccgccgg aaagctggaa ataggccact tcatcagcag gtgatgccgt ggcagtaaaa
     9421 ccgtctgccg gatggttaat cgcatcctgc aagttatgct caccgctgtc gttgtgcagt
     9481 tgcaccacgc gaatggagga atgttctgtg acgaaagtat tgaggaaatc atccccgcta
     9541 aacagcgcat gttggcgatc ggcaatcagc aatgcgggtt caatctggct ggcataggcg
     9601 ttcagttcac tacgctgatg gctgaacaac gccagcaccg gcgcaacgcc cagtttcagc
     9661 agcgcgaaaa aggtaatata caattcagcg acgttaccca gttgtaccag cgcggtttca
     9721 ccaggtttaa tgccctgacg gcgtaaagta cacgcgaggt tatccgccgc ctgattcagc
     9781 tcccggtaac tcaactgtag ctcgccgtcg ataaccgcga tgctgtcact cgcagcgtgg
     9841 cgagtcagaa tgtcggtcag cggcaaatcc tgccagtagc ctttttcccg atagcgacgg
     9901 gcaaactctt ccggccagcg ggtgaatgga atgctcatcc tcgctcctta atgcaatcca
     9961 aaaacattca acatggtaga aagtttgacg cctgtttcgc gccactcacc caacggtgac
    10021 gacgcaagca caatccccgc tccggcaaac agacgcacct gattttcccg cagcttcgcg
    10081 cagcggatgg tcaccaccca ttcgccgtta ccttcgctgt cacaccaacc cacaatgccg
    10141 ccaaacagtt cgcggtcgaa cggctccagt tcagcaataa cctgagtcgc ggcctgatgc
    10201 gggaaaccgc tcagcgcggg ggtcggatgc agcagacagg ccagagtcag tgcgttttct
    10261 tgcgaattcg ctttaccttc aaagggagtt gcgagatgcc acagcgtcgg cgtggtaatc
    10321 aattgtggag aggagggaac gtgtaactca ctactgcgtt cgcgcagtac ctctttcatc
    10381 gcctgagtca ccagttcatg ttcatggcga tctttttctg acgccagcag acgattaccc
    10441 gcttcgcgat cgagcacttc atccggctga cgacgcgcgg aaccggctaa cggaatggag
    10501 ctaaaacgct cgccgtcttt acgtagcagc agttccgggc tggcccccag caggacgcca
    10561 ccatcagcca gcgggacatg gaagttgtaa ctaaccgggt tttgcgcaat caaccgttcc
    10621 agcaatacgc cactatcaat ggcggcgtca gtggtgatat caatcaaccg tgacaacacc
    10681 actttgtcga cctgcggcgt ggcggtaagt gcggcagcgc gggcaaccat ctgttcaaac
    10741 gtggtttgtt ccggaattgc ctggcgttcc accacattca gcgactggct gcgggtgaaa
    10801 cggcgggcgg aagcttgttt ttcctgacgg gagaacgact gccaggattc aggaatatac
    10861 aacgacgaag gctgacgtgg atcgaaggga atcgccccga ccatcaccgg atttttgatg
    10921 ccctgcactt tggcatcggc aaacagcgcg gcgagttttt gctggaaggg actgtcgggc
    10981 gaatccccgt tcacagccgg ttcatcgaag cgggcgaaac atcctgacgt cgtaaaactg
    11041 cggtacggcg acataaagaa aaagcgattg ggcgcaagtg ttgccatggt ctgctgtact
    11101 tcctcagcca gtgacgtatc catatcatcc tccacaaaat gataaaggct ttaataatga
    11161 ttatcattta tattttcggt cgctaaccta aaagcaaacg cgcactatgt caactcttga
    11221 ggtaacgcaa tctgcaactc ggggttgcat ctggttgtgg ttacaatgaa aatgagaagc
    11281 attattgatg gattcgcata agcgcaatgt gatgtcctgc gccgttcttc cccctctccc
    11341 ttccagggag agggctgggg tgagggtcaa tattcgcacc agtgctggct gctcccatca
    11401 ccctaaccct ctccccaaag gggcgagggt acggatagtg cgctttgtcg aatttgtcat
    11461 tacgccctta accttattaa taacaggaag ctgatttgtg agactcgccc cgctctaccg
    11521 caacgccctt ctattaacag gacttttgct ttccggaata gccgcagttc aggccgccga
    11581 ctggccgcgt cagattactg acagccgggg cactcatacc ctggaaagcc agccgcagcg
    11641 tattgtttcc accagcgtca ccctgaccgg ctcactgctg gcgattgatg ctccggtgat
    11701 tgccagcggc gcgaccacgc caaataaccg cgtcgcggat gaccagggct ttttacgcca
    11761 gtggagcaag gtggcgaaag aacgcaaact acaacggctc tatatcggcg aaccgagcgc
    11821 cgaagccgtt gccgcgcaaa tgccggatct gattttaatt agcgcaaccg gcggggattc
    11881 ggcgctggcg ctgtatgatc agctttccac catcgccccg acattaatca tcaattacga
    11941 cgacaaaagc tggcagtcgc tgttaacgca gcttggcgaa attaccgggc atgagaaaca
    12001 agcggcagag cggattgcac agtttgataa gcaactggca gcggcgaaag agcaaatcaa
    12061 attaccgccg cagccggtca ctgctattgt ctataccgcc gctgcacaca gtgccaatct
    12121 ctggacgccg gaatcagcac aagggcagat gctggaacaa ctcggcttta cgctggcgaa
    12181 attgcccaca gggttaaacg ccagccaaag ccagggcaaa cgccatgaca tcattcagct
    12241 tagtggagaa aatctggctg cagggttaaa tggcgagtca ctattcctgt tcgccggtga
    12301 tcagaaagac gccgatgcga tttatgccaa tccactgctc gcgcacctgc ccgcagtaca
    12361 aaacaagcag gtttatgcgc tgggaaccga gacgttccgc ctggattact acagcgccat
    12421 gcaagtgctg gaacgactta aggcgctgtt ttaagcatta actgtcggac gctgtcacct
    12481 gcggcggcgt ctggcgaaaa cgtcgcaact ccaccagcac cagcaataac aacacgccga
    12541 taatcaacaa accaaaaccg ctcgcgcttg cggaagcaac cggtgtcatc atcgctccca
    12601 gaccgcctaa cagcgccgcg cctatggcat cgcccgtcac gttttgtgcc gtccacaaac
    12661 cgttgattcg ccctaacatt gcttccggcg tttgcgtttg cagcattgta tattgcagca
    12721 acgaactgac cgcactcagc cagccgaaca gcgccagaca aaccacgcct aaaatccaca
    12781 tcggcatcag gccaaataaa ccaatggcga ggaacgatcc caacgtggag agcaacatca
    12841 acaatcctgg tcgcgcgcta tgcgccagtt tcccactggt taatgcgcca atagccgcac
    12901 cgagcgggat cgccgcatag agaaaaccaa tctctgccgc cgacatctgc cagttgtcag
    12961 ccagcgccgg atacagtacc cgcaccgcgc tcgccatcgt caataaacca cccagcaacg
    13021 caatcccgcc caccagcggg ctggcgagca gaaaacgaaa tcctgccagt aatgatttca
    13081 acggatgctc gcgcggctgc ggtggcggtg gcaacgccgg aaggcttaac aacggtagca
    13141 aggtaataaa cgtgcccgcc gccgccagcc cgtagttcca ggctacgcca ccggtcgcca
    13201 gcaataaacc gccaatcatg ggcgaaatca ccgaccccag acgcacggtc aacatggtga
    13261 tcgccccggc ctgcattaag ttttcacgcc ctaccagtgc tggtgtcgcc gccaatagcg
    13321 ccgtaacgcc aagcgatgcg aaaaaaccat cccataaacc aagtaaatag attgccagca
    13381 atgacggctc cggcagcagt gcattaaggc acagtccaat gaagccaatg ccacaggtgc
    13441 cgcgcgccag caaaatcact tttttgcgct catagcgatc cgccagcaca ccgccaacca
    13501 tcaggccaac aaacatcgcg ccgccggtca gcgtcaccga aagccccacc agccaggtag
    13561 aatgcgtcat catctggatc tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa
    13621 ttgagataaa acgagcgagg aatactgcgc gaaacgccgg gtgcgttttc aacaggctga
    13681 ggttaagcag ccaggattgt ttattcatta caatgccttg ccatcaaata ttttaaatac
    13741 ctattcttag gctgcacatg ctaacatatc caaataagat cgataacgat aattaatttc
    13801 attatcatgg aagttcgtat gtctggttct gttgccgtga cacgcgccat tgccgtgccc
    13861 ggattgctgt tattactgat tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca
    13921 ctccccgctt ccgtagtgct ggaggccttc tccggcacct gccagagcgc cgactgcacc
    13981 atcgtgctcg acgcgcgact gccgcgcacc cttgccggtt tactggcagg cggcgcgctt
    14041 ggccttgccg gggcattgat gcaaaccctc acccgaaatc cacttgccga ccccggcttg
    14101 cttggcgtga acgccggagc cagctttgcc attgtgctgg gtgcggcgct gtttggttac
    14161 tcatccgcgc aggaacaact ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt
    14221 gttgccttta ccggcagtca gggcggcggg cagttaagtc cggtgcgttt aaccctggcg
    14281 ggcgtggcgc tggcggcagt gctggaaggg ctgaccagcg gcatcgccct gcttaatcct
    14341 gacgtctacg atcaactgcg tttctggcaa gccggttcgc tggatattcg caatctacat
    14401 accttaaaag tggtgctgat cccggtgcta atcgccggag caaccgcgct attactgagc
    14461 cgcgcgctga acagtttaag cctcggcagc gacaccgcga cggcgctggg cagtcgcgtg
    14521 gcgcgcacac agttaattgg tctgctggcg attaccgtgc tttgtggtag tgcgacagcg
    14581 gtagttggcc cgattgcctt tattggcctg atgatgccgc atatggcgcg ttggctggtg
    14641 ggtgccgatc atcgctggtc gctgcccgta acgctgcttg ctacccctgc cctgctgctg
    14701 tttgccgata tcatcgggcg ggtgattgtg cccggtgaac tgcgcgtttc tgtggtcagt
    14761 gcctttattg gtgcaccggt gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca
    14821 tgatttacgt ctctcgccga ttactcatca cctgtttgct gcttatttcc gcctgtgtgg
    14881 ttgcaggtat ctggggatta cgcagcggtg ccgtcacgct ggaaacctcg caggtattcg
    14941 ccgcgctgat gggcgatgcg ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac
    15001 cacgcgtgct gatggcgctg ttgattggcg cggcactggg cgtcagcggt gcgatttttc
    15061 agtcgctgat gcgtaacccg ctcggcagcc ctgacgtaat gggctttaac accggggcgt
    15121 ggagcggcgt gctggtggcg atggtgctgt ttggtcagga cctgacggct atcgcgctgg
    15181 cagcaatggt gggcggcatt gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg
    15241 gtatcgacac ctttcggttg attattatcg gcatcggcgt tcgcgccatg ctggtggcct
    15301 ttaatacttg gctgttgctg aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga
    15361 atgccggatc gctcaatggc ctgacgtggg caaaaacttc gccttccgca cccatcatta
    15421 tattgatgct cattgccgcc gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg
    15481 atgataccgc ctgcgcgctg ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg
    15541 ttgcagtggt gcttaccgct gcggcaacgg cgcttgccgg gccgatttcc tttattgctt
    15601 tagtcgcgcc gcacattgcc cgacgcatta gcggcaccgc tcgctggggg ctgacccagg
    15661 cggcgctgtg cggagcgctg ttactgctgg cggccgatct ctgcgcccaa caactgttta
    15721 tgccgtatca acttccggtt ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg
    15781 tcttgttaat tcaggagtct cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga
    15841 acagttaacc ctgggatatg gcaaatatac cgttgcggaa aatctgactg tagaaattcc
    15901 tgatggtcac ttcacggcaa ttatcggacc aaatggctgt ggtaaatcca cgttactgcg
    15961 taccttaagc cgcctgatga cgcctgctca cgggcatgtc tggctggatg gcgagcacat
    16021 taaacattac gccagtaaag aagttgcacg ccggattggt ctgttggcgc aaaatgctac
    16081 cacgccgggc gatatcaccg tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc
    16141 gctgtttacc cactggcgca aagaggatga agaagcggta acgaaagcga tgcaggccac
    16201 gggaataact catctggcag atcaaagcgt ggataccctt tctggcggac aacgccagcg
    16261 agcgtggatc gcgatggtgc tggcccagga aacggcaatt atgctgctcg acgaacccac
    16321 gacctggctg gatatcagtc atcagattga tctactggaa ctgttaagcg aactgaaccg
    16381 cgagaaaggc tataccctgg cggcggtgct gcacgatctt aatcaggcct gtcgttacgc
    16441 cagccatttg attgcattgc gggaagggaa aattgttgct cagggagcgc cgaaggagat
    16501 tgtcactgct gaactgattg agcgcattta tggtctgcga tgcatgatca ttgacgatcc
    16561 ggtagccgga acgccgcttg tggtgccgct cgaacgaacg gcaccttcaa ccgcaaatag
    16621 ttaaactaag tggtctgcca tcatggcatc ctgttttctg gatgccatcg catagcgcaa
    16681 taacacgcta ccacaagcca ccatcccgcc gatcagcgcg gaaaggatca caataatcgc
    16741 cttacccgga ccgtcttttt tcactggcaa tgacgggctt aactgatatt taaacggcgt
    16801 aaaattcaca tcgttgacat ttgcttttgt taattgctcg acaagatact gccgattacg
    16861 taattcaccg ttcagttccg caacgtcagt aaccgctttt tcaatttcca gtttgcgttc
    16921 aataccgtct gcgccgagag aaatagagaa atcggggtca tctttaacgg cctgaccatt
    16981 actgtatacg ggctttttaa ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg
    17041 ctgaatgttt gcatcaagtt gatttttcgt tttaatgcga tcctgagcca gtttttcttt
    17101 ttcaaactgg gttttgatct ccagtttatt acggacgttt tctatcgact ctttcaccac
    17161 caacgcagag atataatcga tatacccgct caaaacggtc tgcgcctctt tactggttgg
    17221 cgcggtaaaa cttagcgtcc aggaggtata cagtgacggt tcatcttttt tcttactggc
    17281 attgtcatca accgctttca ttttttcgct caatgcaaca atagcgcgat gcaaatccag
    17341 ttcatcgatt ttcgcctctt ttaattggtc catcacataa ggtgatgaac gcaggtactc
    17401 ttccagcaag ctaacagact gaaacttctt gataaacagg ttaaatgcac cgccacggtc
    17461 gatattaaca tcgagatcaa gaacgcggag tttggtaaag gttttctcca ggtcctgcca
    17521 ctgaatggct tcggcgggag taataacggc tgagctggtc catttttgcg gcaggatgaa
    17581 agagatcagt aaacctgcgc aggcaaatgc aaaaacgacc gccatgaccg tttttttggc
    17641 ccgccataaa acttcgatta gattaagtaa atcaatttca ttactactgg gcgacgccag
    17701 aggatactcg gaaaaatgag cgtcacttcc ctgtttaata ttcagtgatg acatgggtat
    17761 aacctgaaaa tgagtccaat agggggaaat ctccacggcg ctcactttac cagttggtga
    17821 gaattatccg aagctgaagt ttgtaaattc cttccactga atgcaacatt cactctattt
    17881 aagagaaata ttcatcaaac ctataaatca aacaaataaa aaacccataa ttattttctg
    17941 taattatggg tttatagata atattaattt acctgtttag cgttgcgcga ataatcggcc
    18001 caatttttac aaacgcccct ggagagataa tatccacatg cgcacaatcc tgacgataga
    18061 tatccagctc ggcaatccag ggcgaccaag cgcgttcggg gctcatacct tcctgaagcg
    18121 tgcgttcagc aacaaacagc gtcgctttac catcaaacgg tacgctatga gcagtcgtca
    18181 gcagacgcac agcatcagcg tagttgcctt caatggtggt aaacaactcc gttgaagtac
    18241 ttccctgctg tgccgccagg aaggcctcgc gttcgcggtt aatctccgcc agcacttccg
    18301 ggtccagacc attagcctct ttttcctgcc agttttgcgt ttctggcggc caggtatcca
    18361 gcaaaccaag aaatgccacc tgttcgccac gggcacgcag tcgcgccgca atgccctgcg
    18421 ccagcgtacc gccaagggaa taccccagca ggtaataagg gccgtgcggt tgttgttcaa
    18481 gtaacgttgc cagatgcgct tcacagactt catccagatt tgccgcggta tgcatggggc
    18541 cattggggcg cggcgactga atgccgataa tcgaccattg tggatcgata taacgcgaga
    18601 gcacgctaaa ctgccaggca aaaccggatg ccggatggaa acaaaacagc gtcgggccat
    18661 taccttcacg caacggcaga atggtttcga atcccatacg ccgggtgctg tcttcttcag
    18721 catcaataat cgttgccagt ttagcgacgg ttgacgcgac catcacttgc cccggcgtca
    18781 cctggcgggc aacctgccga cttaactgcg ctgccagttt cattgccagt agcgaatgac
    18841 caccaagcgc aaagaaatca gcatcggcat cctgcacgtc acaacccagc aacgacgcga
    18901 atgccgcggc aataatcgtt tcactgcccg cttttggcgc acgccccggc gcttgtgcct
    18961 tcagttcagg caacggtaag gctttgcgat ccagcttgcc gttggcgcta agaggtaaca
    19021 gtggaagttg cagcagaacc acgggcacca tatgtggtgg caatgtttcg cgaagctgcg
    19081 cctgtaatgc gctggtatcc aacggcagac ctgattgcga caccagatag cccaccaact
    19141 gacgcgcatc accaccggtg gctgccgcct ggttaatcac acaggcgtgg gtaacggctt
    19201 gttcgacatc cggcagcgcc tgcatcacgc gatcgatttc gcccagttca atacgctgcc
    19261 cgcgaatttt tagctgatcg tcactgcgcc cgaggtactc caccgcgccg ttatccagcc
    19321 agcgggcaac gtctccggta cggtacatcc gttcaccagg gacaaaagga tcggcaataa
    19381 agcggctggc ggtcagatcg ggtcgtccaa gatacccctg cgccagttga atgccggtga
    19441 gatagagatc acccgccaca cccggcggca ccggatgcat catcgcatca agaatacgca
    19501 gacccgtatt ccataccgga taaccaatcg gcacactgct gccgcgcacc tgtgccagtt
    19561 cctcgccaaa agccggatac cagctgacat ctaccgccgc ttccgtcggg ccatatagat
    19621 tatgcaacgg cacgctggtt aactgttgcc attcgcggca taaatcagcc ggtaaggcct
    19681 caccactaca gaaaacctgt ttcaacgtcg cgcaactctg gcgagcggtt tgcggcgtca
    19741 gcgatgcaac aaatgctgcc agcatcgacg gcacaaagtg cgtggtcgtt acgccatatt
    19801 cggcaaagaa ttgctgcata gcgagcgggt cgcggtgcgc ttccggttca gccatcacca
    19861 gtttagcccc ggcaataaac ggccagaaaa actcccacac cgagacatca aaactgcacg
    19921 gcgttttttg ggcaacgaca tcttcgcctg taagcagata atgattttgc atccacaaca
    19981 ggcggttaac gatagccgtc tgcccgacca ttaccccttt cggcctgccg gtggagccag
    20041 aggtaaagat gatataagcc gtgtgatgcg gttgtgaaag ttgcagcggc gcactgccct
    20101 gcggtttaag cggggcgtta tagcaaaggt tggttaaatc tggaacatcg gcaaagcgcg
    20161 gcagttgatc gtcggtggtg attaacagcg acggacgcgc atcttccagc atcattttca
    20221 ggcgatcgtc cggatagccg gtatccagcg gtagccaggc cgcgccagct tcaactatcg
    20281 catgtagcgc cagggtcaaa aagaccgagc gcggtagcgc caccgccacg ctgtcgcccg
    20341 gtttaacgcc acgctcacgc agcagattcg ccagcgccac cacctgctcg cgcatttccc
    20401 gatagctgaa ctggtaacgc gcatctgcca gcgccggagc atccggtgtt tttgccgctt
    20461 gttctgccac cagcgcgctc agcgtggttt ctggaatctc aacctgagtg gcgttgatct
    20521 gcgccagctg cgcatactca cctggcagca taatatcgac atcgccgcac aacagcgccg
    20581 gatccgcggc gaactgggca atcagcattt tcaggcgttc agcatgctgg attaacgttg
    20641 gctcatcgta acgctgttta ttggcgagga tctcaatact caaatcaccg tgttcatccg
    20701 ggaacagggc cagttcaagg tcattaaccg gaccggttgc caaggtatgg gtttgcgcct
    20761 gaacaccagg aatatccagt tggtaatcaa ataccttgat attgagtacc ggaccaaaca
    20821 gcggttcatc acctgccgct cgcccgctgt cacggacaat ttgttcggca tcgtaacgtt
    20881 gatgacgacg catttttttc agttgtgctg ccagtcgggt tgccagttcc ggcagcgttt
    20941 cttgcgccgc aatgtgaata cccaacggca aaacgttgag cacgggtccg gtagccgtca
    21001 gcgccgccga gcccagtcga cgcataaaga taaatccagc ggcgtagtcc atgcgattgc
    21061 acaatcgccc cagccacaaa gccgtcagcg caagggctaa atcggtacgc tgcacacctg
    21121 aaagttgcgt agccaactgg cggaattccc cgtcggtaaa ttccattttc agacgtaaaa
    21181 tatctgccga agcgctgcgc cccggtaaag gtgccggaga aagtgacgcg ggcggcggca
    21241 gttgacgacg ctgttctgcc cagaatgccg catcacgctg ccaggcttcg ctttcgcggt
    21301 attgctggta ctcttccact acatcagcga aaggcgtaaa cggcgaagca ggcgttggtt
    21361 caccacgcag ccatgtgcag taaatattgg cgatctggcg ggtaatggcc gggaaactga
    21421 agccatcgac cagcaaatgg tgataacgct gataccagta ccagcggtta tccgccacct
    21481 gtatcagctg atgaaagacc agtggtttac cgctatcgac gcgcagatct tgttgcaaat
    21541 ccgcctgcat taatgcctgc gcagtaccgt gcggatcaat gttggttcgc aggtcgataa
    21601 tttctggcag ttcgaacgtc agcgcatcat cgacccactg ccagacttcg ccgttatctt
    21661 ccgtaaaacg catccgcagc gtatctgctt gcgctagtcc gtcaaccacc gcgcgggcca
    21721 gtaatggcgc atcaacctct ccggttaact caacgtaatg cgctacgctc caggcggagg
    21781 gtaaatccga cagtttttct gccatccaga tgccgggctg tgcggcgacc aaaggtaaat
    21841 gctggctcat tgcgcctcct gcaactgggt gaaattcgcc ggtgtcagag tacgccagtg
    21901 ggcttcaagc cactgctggc aggactcttg tgactgcggc tgacacacaa tgtcccagcc
    21961 tgccggtaag gcgcattgtt gcggccacag actgaattgc ccctgcgcat tgcgcaaaat
    22021 gtaaaacgct ccctgtggat catcgaaggg attactgaat gccatattca actcctgtca
    22081 tggaaaagtg gttgccagag gtcgattagc ccctgcatca agccaccgcg ccaacaaagc
    22141 gcatcatgtc cgccgtcaac ctgacgccag aaaatggatt cttttatggg gtgtaattgc
    22201 gcatacagcg cctgattggc ccgcataatc atcggctcgc gaatacccgc ttccagcaca
    22261 atgcgcagac cttcggcgct aacttcacca gctttaagct tttcaagtaa cacgccctct
    22321 tgctgcccgc cccgatgcgg ccaccagtac gatcctgact ggcttaatac acaaccaaaa
    22381 cgttcaggcc agtgcagtcc ggcatacagc gcggaaagtc caccaaaact ctgcccggca
    22441 accacggtgc gatcggcgcg atcgctaaaa ggggcaatag ctttcaccag gggtaataac
    22501 tcttgctgta ctgcgagcca gaaatccgca ttacacggca gttcgtgagc gcggtgcgtg
    22561 gtgtcgatag cgtcgatcaa cacatacacg gcgggaggaa gttgctgacg atgggtcagc
    22621 gaagtcagca ctggccagac gggcatactt tgcgcccaaa attcgccatc gagcaaaact
    22681 gccagcgggc gttcttctgc tgttgcatcg ccggtggtaa aaatccatac acgacgtgaa
    22741 tttttcaacc gttcactttt ccagataatt tctttggctg gtatttctgg cgcttgcgga
    22801 caatcccatc ccggttgcag aggcgcttgc ggcatttcga gtgcagaaac agcgtgccct
    22861 cgcccgcctt tccagctttg tggattcagc ggatcggcta ttgcctgggg taatagtttt
    22921 cgccagcctt cacgtaattc gaggcgatcg ggggatggtg cagaaaaaat gtcatcgcgt
    22981 tcggtgggaa taaagcagta gctgccgcgc cagttggcat tgagttgtgt cgtccactgc
    23041 cagacatcag tacctgcaat gcgctgcatc gactggggct ggctgttctg atgatgatcg
    23101 gtcacaccag tgatgtagac ccatacgcgc tttatcggcg agtattcttc agaaccttgg
    23161 ggctcacgcc accagaaagt gacctcaaac atttcgtcat tcagacgctg ccattccggg
    23221 ccatgtttcg actgccacca gctctcactt cctactttta acgccgtcac accataaccc
    23281 catgtttact gtgcaatttt tcattgattg cagaaatata ttgataatat tattgataac
    23341 tatttgcatt tgcaatagcg taatggcgcg ccgtgggaag cgcggacatt aattaaccaa
    23401 ctgcactgcg tgtctttcag gatcaaaggt tttcgcggta gcgggatgcg tcgtgttgag
    23461 gacgaccatg cccgacagtt gcaattcgtg gcaaaaatgc aggaataaaa caatgaacaa
    23521 gaagattcat tccctggcct tgttggtcaa tctggggatt tatggggtag cgcaggcaca
    23581 agagccgacc gatactcctg tttcacatga cgataccatt gtcgttaccg ccgccgagca
    23641 gaacttgcag gcacctggcg tttcgaccat caccgcagat gaaatccgca aaaacccggt
    23701 tgcccgcgat gtgtcggaga tcatccgtac catgccaggc gttaacctga ccggtaactc
    23761 caccagtggt cagcgtggga ataaccgaca gattgatatt cgcggtatgg gtccggaaaa
    23821 cacgctgatt ttaattgacg gcaaaccggt cagcagccgt aactcggtgc gtcagggctg
    23881 gcgtggcgag cgcgataccc gtggtgatac ttcctgggtg ccgcctgaaa tgattgaacg
    23941 tattgaagtt ctgcgtggtc cggctgcagc gcgttatggc aacggcgcgg cgggtggcgt
    24001 agttaacatc attaccaaaa aaggcagcgg tgagtggcac ggttcctggg acgcttattt
    24061 caatgcgcca gaacataaag aggaaggtgc cactaaacgc accaacttca gtctgaccgg
    24121 cccgctgggc gacgaattca gcttccgctt gtatggcaac ctcgacaaaa cccaggctga
    24181 cgcgtgggat atcaaccagg gccatcagtc cgcgcgtgcc ggaacgtatg ccacgacgtt
    24241 accagccggg cgcgaagggg tgatcaacaa agatattaat ggcgtggtgc gctgggactt
    24301 cgcgcctctg caatcactcg aactggaagc gggttacagc cgccagggta acctgtatgc
    24361 gggcgatacc cagaacacca actctgacgc ttacactcgc tcgaaatatg gcgatgaaac
    24421 caaccgcctg tatcgccaga actactcgct gacctggaac ggtggctggg ataacggagt
    24481 gaccaccagc aactgggtgc agtatgaaca cacccgtaac tcgcgtattc cggaaggtct
    24541 ggcgggcggt accgaaggga aatttaacga aaaagcgaca caggatttcg tcgatatcga
    24601 tcttgatgac gtgatgctgc acagcgaagt taacctgccg attgatttcc tcgttaacca
    24661 aacgctgacg ctgggtacgg agtggaatca gcaacggatg aaggacttga gttccaatac
    24721 ccaggcgctg accggggcga acaccggcgg cgctattgat ggtgtgagtg ccaccgaccg
    24781 tagcccgtat tcaaaagcag aaattttctc gctatttgcc gaaaacaaca tggagctgac
    24841 tgacagcacc atcgtaacgc cggggctgcg tttcgatcat cacagtattg tcggcaataa
    24901 ctggagcccg gcgctgaaca tatcgcaagg tttaggcgat gacttcacgc tgaaaatggg
    24961 cattgcccgc gcctataaag cgccgagcct gtaccagact aacccgaact acattctcta
    25021 cagtaaaggt cagggctgct atgccagcgc gggcggctgc tatctgcaag gtaacgatga
    25081 cctgaaagca gaaaccagca tcaacaaaga gattggtctg gagttcaaac gcgacggctg
    25141 gctggcgggc gtcacctggt tccgtaacga ttatcgcaat aagattgaag cgggctatgt
    25201 ggctgtaggg caaaacgcag tcggcaccga tctctatcag tgggataacg tgccgaaagc
    25261 agtggttgaa ggtctggaag gatcgttaaa cgtaccagtt agcgaaacgg tgatgtggac
    25321 caataacatc acttatatgc tgaagagtga aaacaaaacc acgggcgacc gtttgtcgat
    25381 catcccggag tacacgttga actcaacgct gagctggcag gcgcgggaag atttgtcgat
    25441 gcaaacgacc ttcacctggt acggtaagca gcagccgaaa aaatacaact acaaaggtca
    25501 gccagcggtt ggcccggaaa ccaaagaaat cagtccttac agcattgtgg gtctgagcgc
    25561 gacctgggat gtgacgaaga atgtcagtct gaccggcggc gtggacaacc tgttcgacaa
    25621 acgtttgtgg cgtgcgggta atgcccagac cacgggcgat ctggcagggg ccaactatat
    25681 cgcgggtgcc ggggcgtata cctataacga gccgggacgt acgtggtaca tgagcgtaaa
    25741 cactcacttc tgatgctaac gtcagattgt tgataaagtg cgcgtcgttc atgccggatg
    25801 tggcgtgaac gccttatccg gcctacaaaa atcttgccaa ttcaatatat tgcaggacca
    25861 tgtaggcctg ataagcgtag cgcatcaggc agtttagcgt ttgtcaccag tctctaatat
    25921 ggtcgacatg aaaactacgc atacctccct cccctttgcc ggacatacgc tgcattttgt
    25981 tgagttcgat ccggcgaatt tttgtgagca ggatttactc tggctgccgc attacgcgca
    26041 actacaacac gctggacgga agcgcaaagc cgaacattta gccgggcgga tcgctgcaat
    26101 ttatgcgctg cgggaatatg gctataaatg tgtgcccgca atcggcgagc tacgccaacc
    26161 ggtgtggcct gcggaggtat acggcagtat tagccactgc ggggctacgg cattggccgt
    26221 ggtttcccgt caaccgattg gcgttgatat cgaagaaatt ttttctgcgc aaacagcgac
    26281 cgaattgaca gacaacatta ttacaccagc agaacacgag cgactcgcag actgcggttt
    26341 agccttttct ctggcgctga cactggcatt ttccgccaaa gagagcgcat ttaaggcaag
    26401 tgagatccaa actgatgcag gttttctgga ctatcagata attagctgga ataaacagca
    26461 ggtcatcatt catcgtgaga atgagatgtt tgctgtgcac tggcagataa aagaaaaaat
    26521 agtcataact ctgtgccaac acgattaatt gacaacatct ggtacgattc gcccgcagcc
    26581 atcactgacc gcgggcgaaa gtgtaaagca ggtgccttac catcctgacc tgacaaccgg
    26641 atatgcgggg attgctcccc gcacaacggc tacttcttcg gttcgtaagc gagaacagcc
    26701 ttaaactcaa tattacgttc ctttacggtg aactcacaca gcgaatcccc gaccagaagc
    26761 gtaaatccca gcaccgttaa acacagcact atgactgccg caagggcata tttcgtcagc
    26821 atgtttatat tgcctccatg cgaagaaggg actaccatcc aaattgttca ggtttgggta
    26881 ggtagcccca gggttgatag aaatatcgtc ctggggcttc atctttccag acctcagagt
    26941 aaacctgaaa ccggaaagct tcaggcaccc gcagttatct tacccttgct tagcaaaata
    27001 gctgtttatt tatacagtaa gtcctgtttc ggacttctca ctccccattt gatcaactga
    27061 agctgaaccc gtttttttct gcatgcctca atcgtcaaaa ttcttcgcca ccagtcagca
    27121 ctgccataac ttgtcacatt gcaaaagtag ccaaattgct tctgtcataa tcagttcacc
    27181 atcaccaaaa aaaagaaaca ttccagaact tccgcatctc gcccagccgc tcccacttct
    27241 atactgaata gaaaacgcca acataagaga aacctatgcc attacccgat tttcatgttt
    27301 ctgaaccttt tacactcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg
    27361 acttaagcca ggactcttca atactgattg acgcggttaa aaataagatc acggccggag
    27421 aggtaaagca cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata
    27481 tcaaccaggc tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag
    27541 accatcatct ggaaatttgc ggcggtggca cacacccgtt tcagaaatgg cagcgacagg
    27601 aggtatgcga taacgagcgc tatcaacgaa cgctggaaaa ctttggttat ctcattcaac
    27661 aggtgaccgt ttttggtcag catgttcatg ttggctgcgc cagtggcgat gacgccattt
    27721 atttgctgca cggcctgtca cggtttgtgc cgcacttcat cgccctttcc gccgcgtcgc
    27781 catatatgca aggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct
    27841 ttcctgataa tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc
    27901 gctgtctgag ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc
    27961 ccagtcctca ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc
    28021 acgcggtaaa tatggcggga ttaattcagg ctaccgccca ctggttactg acagaacgcc
    28081 cgttcaaaca tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc
    28141 gctatgggct ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg
    28201 aagacacctt gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga
    28261 gcgcgattga ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc
    28321 gcgatttcgt cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct
    28381 gggccggtga ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta
    28441 acttacattt aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat
    28501 tttgctgatg gctttcctct cttgcctgct gctgcccacc cccgcactgg ggctgacgct
    28561 ggcacaaaaa ctggtgacca tgttccatct gatggatctt agccagcttt acactttatt
    28621 gttttgtctg tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg
    28681 gcgacgctgg ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact
    28741 ttgcttattc tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg
    28801 cgaaacgcct ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt
    28861 tcgatgtttt taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc
    28921 cctttatcaa tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga
    28981 gcattaagcc agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg
    29041 aaatctccga agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg
    29101 gtctggctaa acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca
    29161 tttttctcgc ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt
    29221 cacatggagt ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat
    29281 ttgatgccag taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat
    29341 acagcccatc cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag
    29401 gtaaagcgcg tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac
    29461 ttgatgcctc gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga
    29521 agctggttgt tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg
    29581 cgaacgataa aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg
    29641 cagagtggat ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg
    29701 ctctgggtct ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat
    29761 ttggtctgaa agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg
    29821 ttgaagattt taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg
    29881 aagtgtaatt ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt
    29941 tgcataaccc tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca
    30001 ggatttaata tcccaggttg gggatttagc gggtattgag atcgatcaca ccacctcgat
    30061 ggtgatgatt ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg
    30121 ggtggtactg cggaccttcg aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat
    30181 cattacccag aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa
    30241 tattcaggcg gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg
    30301 cgcgcagttg tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat
    30361 tttgaatctg gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca
    30421 ggggattaaa ctgatcggcg caatcctggt cggcattttg atgatctcgc tgctgattgg
    30481 tcagtcgcca gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt
    30541 atttaaagat ccgattcttg gtctggtggc aggtattcag ctttccgcga acgatatgct
    30601 gaaactgggc gactggctgg agatgccgaa atacggcgcg gatggcgcgg tgatcgatat
    30661 tgggttaacc accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg
    30721 gtctctggtt tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg
    30781 tattaagcgc agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat
    30841 gcaacgtctg aataaagcgc acttgttaaa accttattta accagccgcc atcaggaaat
    30901 taatgagtgg aatcgccagc aaggttctac ggagtcgata ttaaacctgc gccgaatgac
    30961 caatattgga acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg
    31021 taaagatatg accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga
    31081 gatctacgcg tttaccaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat
    31141 attcgatcac atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccagc
    31201 cggcaatgat attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc
    31261 atcgaatgga tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga
    31321 cgcagcgttt taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac
    31381 aagcgcatat catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcaa
    31441 agatagttgc cgaacggata aagcagcgcc ttaaactgtg tttcacgtcc ctgacgtcgc
    31501 atcgccgcgc gaaaacgcag atgcgccagg cagatcataa tccagttcaa cagcagcgtt
    31561 gctaccacca gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac
    31621 accaccagcg aagtgatcgc tccggaaagc atcagcgagt taatcggcac gccgcgatgg
    31681 ctgacgcgag tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg
    31741 ctgttagagt aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc
    31801 agcgcagaag ctacctcgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg
    31861 ttggatttca cttccaccca cggatagagc gccagtaaaa ccaccagtga acagatgtaa
    31921 aacagcagga tgcgatacac cacctgattt accgcttttg gaatgctttt ttccggatcg
    31981 cgcgcttcag cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc
    32041 gccagcgaca aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg
    32101 ttgtcgatac tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg
    32161 atcataccga tgattgccag cactttaatc aacgcaaacc agaactcggt ttcgccatat
    32221 aagcgcacgt tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc
    32281 cacgttggaa catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc
    32341 attcccacca gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt
    32401 ccccagtatt tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc
    32461 tcgccgagct ggcgcataat caggaaagcg atgatcccgg cgacgccgta gcccagcaat
    32521 acagccggac ccgccatctg aatcgccggg ccaatgccaa gaaacagacc agtaccaatt
    32581 gcgccaccca gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc
    32641 tcttgatttg acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt
    32701 gtgtcttttt tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat
    32761 ccacacggtt aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca
    32821 tcagcttata cgccgccggg ataataaaca gcgacagcaa aggcgcggtg atcatgccgc
    32881 caatcatcgg tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa
    32941 tcggcagcag accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta
    33001 ccgcgccgtg atacaacgcc tcatccagct tctgctcact gaatgtttgc ggattattca
    33061 acgacggctc ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg
    33121 cggcgccccc ggcaagggca ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca
    33181 tccaccacag caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca
    33241 acgcttcgcc cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca
    33301 tcggcaccat gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga
    33361 atgccacgct ggtgcccggt tttaactgca ctttttcaac tatcgctttt tgcaaatcgt
    33421 gaaccaccga caccatgtca cgatcgcggg catcgatata aatccagctc gtcgggcgcg
    33481 cattctcggt tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca
    33541 gggtgatttg ctgcttcatc ggcgtcagga tcggtagctg gcgcagtgcc tgctttgcgg
    33601 ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa ccatcgcccc
    33661 gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt aacgcgcggc
    33721 tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct cggcaagcgc
    33781 agaagccacg cctggcaccg ttcgcgctac ttcttcaatt tgctcagcca tcgcgtcgat
    33841 atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc cggttgagag
    33901 catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg gcagccgcac
    33961 ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc actgatcctg
    34021 cggcttaagc tggatggtcg tttcaaccat ctcgagcgga gcagaatcgg tggcggtttc
    34081 cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa ttagcttgtc
    34141 gtttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg tcgatggcat
    34201 atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt tattgagcgg
    34261 ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg gccagtgcag
    34321 tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg ggttactgct
    34381 ttccggcgga attttgccac ggatccagta acccatcagg atcgggatca ctacgatcgc
    34441 cagcagcgcc gcacccgcca tcgcatacgt tttggtgaac gccagcgggc caaacagacg
    34501 cccttcttgc ccttccaggg tgaagattgg gataaacgac agcgtgataa tcagcagact
    34561 gataaacagc gccggcccca cttcaacaga tgcatcggta atcacctgcc agcgcgtttt
    34621 attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt tatgcgcatt
    34681 ctcgatcatg acgatagcag catcgaccat cgccccgacg gcaatcgcaa tgccacccaa
    34741 cgacataata ttggcattca gtccctggaa gggcatgaca ataaaagcaa tacacaaccc
    34801 cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa acagcgcaca
    34861 gactaccgcc accacaataa actcttccag caacttgccg ctgaggttgt cgatggcgcg
    34921 gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg gcagactact
    34981 tttcagcgtt tccagtttgt ccttcacggc ggcgatcact tctcgggcgt ttttaccgga
    35041 gcgcaggatc accaccccgc ccgccacttc gccttcgccg ttcagttcgg caatgccccg
    35101 gcgcatctcc gggccaacct ggaccttcgc aacatcgcgc agataaacgg gcacgccatt
    35161 ttcactggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat agccgctggc
    35221 gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt cctggtttga
    35281 agcatccagc gcgcttttta cttcggcgag actgatgcca tactgcgcca ggcgctgggg
    35341 atcgataacc acctgatact ctttcaccac accgcccacc gacgccactt ccgcaacgtc
    35401 agggatggtt ttcagctcat atttgagaaa ccagtcctgt aatgagcgta aatcggccag
    35461 atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa cacccgtggc
    35521 atctggcccc agctcggcgc tgactcccgc aggcagctta ccctgtacct ggttgaggta
    35581 ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa tgacatacac
    35641 ataagagtcg ccaaactgcg agaaaccacg cacagtcttc gcgccaggca ccgacaacat
    35701 ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct gcccgggata
    35761 gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca ctggcgtatt
    35821 aatgatggtc caggtgcccc agatgctaag aaacaacgcg cccatcagca ccaaaaaacg
    35881 gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca atgcgcatgg
    35941 gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc ggaatcaatc
    36001 aggaaaaggc cgctggaaac caccttttca ccttccgcca gaccagagcg taatgcggtg
    36061 acaccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgccc gtcggcatca
    36121 atggtaatca cccgctgttc gctaccggta tcaatcagcc cttgtgacgg aatgagcagc
    36181 atcggttcgc tggcggtatt gagttgcaac caggcgttca tgcccggttt tagcgcctcg
    36241 tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc cacgccgggt
    36301 agcag
//
LOCUS       DAJTYD010000022        35011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTYD010000022 DAJTYD010000000
VERSION     DAJTYD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.022"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="OO585_000976"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="OO585_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO585_000977"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO585_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6628642.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO585_000978"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO585_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6628643.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="OO585_000979"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="OO585_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6628644.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGASAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="OO585_000980"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="OO585_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6628645.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO585_000981"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO585_000981"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6628646.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO585_000982"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO585_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6628647.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO585_000983"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO585_000983"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6628648.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO585_000984"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO585_000984"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6628649.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="OO585_000985"
     CDS             complement(14507..15139)
                     /locus_tag="OO585_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6628650.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="OO585_000986"
     CDS             15435..15710
                     /locus_tag="OO585_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628651.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="OO585_000987"
     CDS             complement(15787..16029)
                     /locus_tag="OO585_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6628652.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="OO585_000988"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="OO585_000988"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6628653.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="OO585_000989"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="OO585_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6628654.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO585_000990"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO585_000990"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6628655.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21105
                     /gene="ypdA"
                     /locus_tag="OO585_000991"
                     /pseudo
     CDS             19409..21105
                     /gene="ypdA"
                     /locus_tag="OO585_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
     gene            21120..21854
                     /gene="ypdB"
                     /locus_tag="OO585_000992"
     CDS             21120..21854
                     /gene="ypdB"
                     /locus_tag="OO585_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6628656.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21867..22724
                     /locus_tag="OO585_000993"
     CDS             21867..22724
                     /locus_tag="OO585_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6628657.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO585_000994"
     CDS             complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO585_000994"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6628658.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO585_000995"
     CDS             complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO585_000995"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6628659.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO585_000996"
     CDS             complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO585_000996"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6628660.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27384..28631)
                     /locus_tag="OO585_000997"
     CDS             complement(27384..28631)
                     /locus_tag="OO585_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6628661.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28653..28979)
                     /locus_tag="OO585_000998"
     CDS             complement(28653..28979)
                     /locus_tag="OO585_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6628662.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO585_000999"
     CDS             complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO585_000999"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6628663.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30367..31623
                     /locus_tag="OO585_001000"
     CDS             30367..31623
                     /locus_tag="OO585_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6628664.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31738..32064
                     /locus_tag="OO585_001001"
     CDS             31738..32064
                     /locus_tag="OO585_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6628665.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERSYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32205..33443)
                     /locus_tag="OO585_001002"
     CDS             complement(32205..33443)
                     /locus_tag="OO585_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6628666.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKTLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVLPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33779..34981
                     /gene="nupC"
                     /locus_tag="OO585_001003"
     CDS             33779..34981
                     /gene="nupC"
                     /locus_tag="OO585_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6628667.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9233 a         8693 c         8082 g         9003 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagtcg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaaggggcaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttaaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaat
    20941 gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa gttattgtat
    21001 ggcgaggggc tgcatatccg ccgcctggag ccggggacgg aaattgcgtt ttacattcct
    21061 aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg agtgatattg
    21121 tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta
    21181 aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt
    21241 ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg
    21301 gcgtgttgct ggcgcaaaac atcagccagt tcgcccataa accgtttatt gtgttcatca
    21361 ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac tacattctca
    21421 aaccgtatca ggagtcacgt attaccggga tgctgcaaaa actggaagcg gcctggcaac
    21481 aacagcagac cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc
    21541 tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac gccgaagcgc
    21601 acgagaaaat gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta
    21661 ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc tcattttgtg
    21721 tcaatctgaa caaaatacgc gaaatcgaac cgtggtttaa taacacctac attctgcgac
    21781 tgaaagatct ggattttgaa gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt
    21841 taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat
    21901 ttttcgccga tctgttcagc ggcctggtgc ttaactcgca actactgggg cgcgtctggt
    21961 ttgccagcca gcctgcctcg ttaccggtgg gcagtttatg tattgatttt ccccgtctgg
    22021 atatcgtgct gcgcggcgaa tacggcaacc tgctggaagc aaagcagcag cgaatggtgg
    22081 aaggagaaat gctgtttatt ccggcacgcg cggctaattt accgatcaac aacaaaccgg
    22141 tgatgctgtt aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc
    22201 gcaccacgtc gttgttgcat cctgctcgcc agatccagct tcccagcctg caacgcggtg
    22261 aaggtgaagc gatgcttacc gccctcactc atcttagccg ttcgccgctg gagcagaata
    22321 tcattcagcc actggtgtta agtttgctgc atctttgccg caacgtggtg aatatgccgc
    22381 cgggtaattc gcaaccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg
    22441 ataattatac ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccca
    22501 atcatctgtc aaaactgttt gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt
    22561 gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatctgtca attcatgaag
    22621 tggcacagcg ttgcggtttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt
    22681 ttggtctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt
    22741 ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac gactggcgga
    22801 agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt
    22861 cgttagcacc cagcgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt
    22921 caatcggttt cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg
    22981 tgtgcgggat cgctacgcca aaaccaacgc cggtggtaac aatctcttcc cgctgccaga
    23041 catcttcttc cagctcaaac ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca
    23101 ggaactggat cgcctgctct ttattgctaa aatcctgatc aacaaagata ttctccagcg
    23161 ccagcagtgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct tcaatttcct
    23221 gcgcactgcg gcattcacat gcctgacgcg ccagttcccg acacgcctcg ctatccagtt
    23281 gacgaagctg gcttttcacc gccggaatac gcgggctact catactcagc tcatccaggc
    23341 ccagcccaag cagtagcggc agataacggc tttcaccgcc cagttcaccg caaatgccta
    23401 cccatttgcc ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc aggaacgatg
    23461 gcgtaatcgg gttatatagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat
    23521 actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtggtcga
    23581 tgatgtagca caccgacggc acttccacca tgatcccaag cgtaatcgtc tctgcatgac
    23641 gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga
    23701 tctgatcgag actgtggacc atcgggatca tcaactgggc gttgccaaaa ctggcggcgc
    23761 gcaaaatcgc ccgtagttga gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg
    23821 cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga atgcttttat
    23881 cgccaccgat gtccattgtg cggaagataa tcggcttgtc gcccgctgcc agtagcacct
    23941 gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca
    24001 tttcagtacg gaacagtcca acgccttccg cgccgttggc aaacgcgcct ggcgcttcca
    24061 gagcggtgcc gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct
    24121 gcgcggcggc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgt gcgacctgat
    24181 aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca
    24241 ccgccggttg cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga
    24301 tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccagttttc tccagaatca
    24361 tgcctgcaag attttttaaa tcgaggctca aaaactggct tggggttaaa tcctcggcta
    24421 ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga
    24481 tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc agataatcac
    24541 tggcagaagc agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca
    24601 gcccttgatg ctgttctgtc atcagacgac ggatattgcc cgcgaattca tcatcctgaa
    24661 tcagcgacaa atgggcgcta aggatagttt tgctttcgcc gtcactctca cgcaattgct
    24721 ggttcagttg ctcggcaagc gttgcaaggc tgtgctccag ccgggtggaa tcttgcgcac
    24781 tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca
    24841 cgccgacgct gcttgccagc acattgccgt acagtaaatc cgggttcagg cggcttaatg
    24901 aacgcggcag cggatgcgcc gtcagttctg cctgcgttgg ctggatgctg tcgctgtcga
    24961 taaagcgcac ctggatgtac tcttccaaca cgcgctgcgc ctgctcttca tcgctgccgc
    25021 taatgttcag gctgcaactg tcattaaata aagtaccggt gccaatcagc gccagcgagc
    25081 ttttcgcatc ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact
    25141 ggctgcactg ttctttaagt tcccacgccg gacgagcatg aagaccgttt ggcaaaggac
    25201 agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg
    25261 tcagttgaac aaccgtctca cgcgtaagac gtttaataag ggcagataaa agctgctgca
    25321 tctgcaaaat gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg cgggttgctg
    25381 gccccatcac cactgtaggc acgccggtgc cggttaagtg caccgctccg ccgtccgtgc
    25441 cgccgttgct gaacatatct gcctgtaacg gcacgccaat ttctgccgcc acggtttcga
    25501 tccacgcggt aagttttggc ggcgcaatca gcgacttgtc gcttaacacc agcatcggcc
    25561 cgttacaaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg
    25621 tatcaagcac aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta
    25681 cccccacctc ttcgctggaa ctggccacca gccacacttc cgcaggcagt tcagcgctgt
    25741 gtagttcgcg cagtaacgtc accagcaggt agcaaccgag gcggtcatca aaggctttcc
    25801 ccatcacccg ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg cgatcgcctg
    25861 gacgaattcc cgcctgcatc acttcgtcat acgagcgcgt accaatgtcc acgcgcatgg
    25921 cgctgacgtc attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac
    25981 gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg ccaaccggca
    26041 gcacatcaat tgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat
    26101 gggcgcagat catcaccttc ggccctgtcg attcattgag gcggatcagc accgatccca
    26161 gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgca
    26221 cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat agcgataaat
    26281 ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc
    26341 ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat
    26401 ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa
    26461 acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac cgaaatagtc
    26521 gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac
    26581 gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc
    26641 aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg tgcgcgtcat
    26701 atcagagcag tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc
    26761 aacaatcttg tcgctggctt tgccgtgcgg cagcaccccg cgccagccgc tggcgacaat
    26821 ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc
    26881 tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc
    26941 ggcaatccca caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag
    27001 cacatccggc gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt
    27061 ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat
    27121 ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg
    27181 cgcacgggct tccacctcaa cgaaatagct cgaatctacc agaatgtgcg cactttcacg
    27241 gctaataacc acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg
    27301 tgaggagaga agcactgcat ccagttgttg ctccttaagc cagtcgcgca gcgaagcgag
    27361 taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc tttacggaac
    27421 atcatcagac gcaggaacac caccatcagc gcagttatga ctgcccccag cgcgatcccc
    27481 gccatataga cgccaaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac
    27541 cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatac
    27601 gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc
    27661 ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt
    27721 ttgccggtta aacgacggtc aataatggtc gccagcccca gaccaatcgg aggaataacg
    27781 atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg
    27841 aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc
    27901 attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacggtacg gataacgccg
    27961 ttgatccagc cgccaaaggg ggtaatgacg tagtacatcg ccagcatgac aaaaatggcg
    28021 gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt
    28081 ttctggttca tccacttcac aaggtaaccc gcgacgatag aaataatcag cgcaccaatg
    28141 aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc
    28201 agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccgggaa cgccagtttg
    28261 ccaccgatag agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacacg
    28321 ccgccgaagg actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa accggagaat
    28381 ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa
    28441 gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt
    28501 gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc
    28561 ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt
    28621 ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct
    28681 tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa
    28741 acgtcgcgtg attcgaaacg ctcattatct tccggggtaa ctgccacgga gtggatgatg
    28801 atggttgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg
    28861 gtttcaattt tcacttcata accggcttct accgccgctt cttccaacgc ctgagcggcc
    28921 ataaaggtgt gagccaggcc cattgggcag gcacataagg caatcagttt cttactcatc
    28981 gttaattcct cattcagtga tagttgacaa gtgaaattat gaacgtgcaa aacaaatcgc
    29041 cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg
    29101 caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaacgat
    29161 tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtgc cctaaggtct
    29221 ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg
    29281 gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcagca cggaaaccag
    29341 aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa
    29401 atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg
    29461 acaacgcgcg gcggcaatcg gtacagctgt cagccagcgc gcgttcggta atatcttttg
    29521 gcttgagatt ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca
    29581 gcccagggcc agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt
    29641 cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg
    29701 gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg
    29761 caccgtaaac cgcaataggt ttaccttcta ccggttctgc gccaccaaac tgaatcagat
    29821 gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca
    29881 gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg
    29941 tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct
    30001 cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc
    30061 ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg
    30121 cgttggtgcc gcccacatca ccgactaatg catactttat cattcttcaa ctgctccgct
    30181 aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga
    30241 aaggcaggct ccctgtaaat atcgatctgg gtcacacatt tactttatcg tttcagcacc
    30301 aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa
    30361 tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca
    30421 attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag
    30481 aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg
    30541 atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag
    30601 ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct
    30661 gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg
    30721 ccggaacatc cgatcatgac catcaatatc gcccttgccg tcgccattgg cgctcgtctg
    30781 ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg
    30841 ctgtttggca cacctgttgc agcggcgttg atattttcgc aaaccttaaa tggcagtagt
    30901 gaagttccgc tatgggatcg tctctttgcg ccgttaatgg cggcagcagc tggtgcacta
    30961 actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg
    31021 gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg
    31081 gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg
    31141 ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg tggaccagtt
    31201 tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc
    31261 agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt
    31321 ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg
    31381 ctgcatgagc acgtacccgc cgtaccagcg gcaataaccg tttcttgcgc tattctcggc
    31441 atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc ggtcgttgta
    31501 cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta
    31561 gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt
    31621 tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct
    31681 ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg
    31741 ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaacgca
    31801 ggtgaaatca ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt
    31861 aattactggg acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgagtgg
    31921 cgcaaaaacc gttggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa
    31981 gcgtatgagc gtagctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc
    32041 cgcggacatg gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac
    32101 gctagcgcgt cttatcaggc ctacaaaccg gatctttaac gcgtagggcg gataaggcgt
    32161 ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    32221 gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    32281 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    32341 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    32401 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta
    32461 cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc
    32521 agtgtcccca ctaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    32581 gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca
    32641 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca
    32701 aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    32761 cgcgaaccgc catgtaaatg ctgagtgagc gaggagtgca aataaatcac atgcggcata
    32821 atcgtcgccc ctaacacgcc tgctgccagg aagaccgctt ccgaagtagg taaactcggg
    32881 agcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    32941 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgt tttttgcccg
    33001 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    33061 aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    33121 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    33181 tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg
    33241 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    33301 aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    33361 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    33421 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    33481 gctatgataa acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctgtga
    33541 tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    33601 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat
    33661 cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    33721 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    33781 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt
    33841 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    33901 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    33961 cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag
    34021 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    34081 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatca tccgcgcaat
    34141 tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag
    34201 ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat
    34261 ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat
    34321 cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtattgaa
    34381 catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga
    34441 agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    34501 atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat
    34561 cgccctgatc gctgcgctga acgcactgtt tgccaccgtg actggctggt ttggctacag
    34621 catctccttc cagggcattc tgggctacat cttctatccg attgcatggg tgatgggtgt
    34681 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    34741 gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg
    34801 catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg
    34861 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact
    34921 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    34981 agaccataca taaaaaagcc ggggataatt c
//
LOCUS       DAJTYD010000023        34944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTYD010000023 DAJTYD010000000
VERSION     DAJTYD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.023"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..4363)
                     /locus_tag="OO585_001004"
                     /pseudo
     CDS             complement(1..4363)
                     /locus_tag="OO585_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4456..5103)
                     /gene="rnt"
                     /locus_tag="OO585_001005"
     CDS             complement(4456..5103)
                     /gene="rnt"
                     /locus_tag="OO585_001005"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6628668.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRSAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5206..5613)
                     /gene="gloA"
                     /locus_tag="OO585_001006"
     CDS             complement(5206..5613)
                     /gene="gloA"
                     /locus_tag="OO585_001006"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6628669.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(5694..6791)
                     /gene="nemA"
                     /locus_tag="OO585_001007"
                     /pseudo
     CDS             complement(5694..6791)
                     /gene="nemA"
                     /locus_tag="OO585_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(6828..7427)
                     /gene="nemR"
                     /locus_tag="OO585_001008"
     CDS             complement(6828..7427)
                     /gene="nemR"
                     /locus_tag="OO585_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6628670.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7530..7769
                     /locus_tag="OO585_001009"
     CDS             7530..7769
                     /locus_tag="OO585_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6628671.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7818..8714
                     /locus_tag="OO585_001010"
     CDS             7818..8714
                     /locus_tag="OO585_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6628672.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            8795..9316
                     /gene="sodC"
                     /locus_tag="OO585_001011"
     CDS             8795..9316
                     /gene="sodC"
                     /locus_tag="OO585_001011"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6628673.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9317..11328)
                     /locus_tag="OO585_001012"
                     /pseudo
     CDS             complement(9317..11328)
                     /locus_tag="OO585_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11328..12185)
                     /locus_tag="OO585_001013"
     CDS             complement(11328..12185)
                     /locus_tag="OO585_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6628674.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12251..12424)
                     /locus_tag="OO585_001014"
     CDS             complement(12251..12424)
                     /locus_tag="OO585_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6628675.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            12625..13059
                     /gene="slyA"
                     /locus_tag="OO585_001015"
     CDS             12625..13059
                     /gene="slyA"
                     /locus_tag="OO585_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6628676.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13106..13573)
                     /gene="slyB"
                     /locus_tag="OO585_001016"
     CDS             complement(13106..13573)
                     /gene="slyB"
                     /locus_tag="OO585_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6628677.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            13847..14956
                     /gene="anmK"
                     /locus_tag="OO585_001017"
     CDS             13847..14956
                     /gene="anmK"
                     /locus_tag="OO585_001017"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6628678.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VIRPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15054..15383
                     /gene="mliC"
                     /locus_tag="OO585_001018"
     CDS             15054..15383
                     /gene="mliC"
                     /locus_tag="OO585_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6628679.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15442..16098
                     /gene="pdxH"
                     /locus_tag="OO585_001019"
     CDS             15442..16098
                     /gene="pdxH"
                     /locus_tag="OO585_001019"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6628680.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16227..17501
                     /gene="tyrS"
                     /locus_tag="OO585_001020"
     CDS             16227..17501
                     /gene="tyrS"
                     /locus_tag="OO585_001020"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6628681.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            17560..18423
                     /gene="pdxY"
                     /locus_tag="OO585_001021"
     CDS             17560..18423
                     /gene="pdxY"
                     /locus_tag="OO585_001021"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6628682.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(18467..19072)
                     /gene="gstA"
                     /locus_tag="OO585_001022"
     CDS             complement(18467..19072)
                     /gene="gstA"
                     /locus_tag="OO585_001022"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6628683.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19178..20680)
                     /gene="dtpA"
                     /locus_tag="OO585_001023"
     CDS             complement(19178..20680)
                     /gene="dtpA"
                     /locus_tag="OO585_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6628684.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21291..21926)
                     /gene="nth"
                     /locus_tag="OO585_001024"
     CDS             complement(21291..21926)
                     /gene="nth"
                     /locus_tag="OO585_001024"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6628685.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(21926..22621)
                     /gene="rsxE"
                     /locus_tag="OO585_001025"
     CDS             complement(21926..22621)
                     /gene="rsxE"
                     /locus_tag="OO585_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6628686.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(22625..23245)
                     /gene="rsxG"
                     /locus_tag="OO585_001026"
     CDS             complement(22625..23245)
                     /gene="rsxG"
                     /locus_tag="OO585_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6628687.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23249..24307)
                     /gene="rsxD"
                     /locus_tag="OO585_001027"
     CDS             complement(23249..24307)
                     /gene="rsxD"
                     /locus_tag="OO585_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6628688.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24308..26530)
                     /gene="rsxC"
                     /locus_tag="OO585_001028"
     CDS             complement(24308..26530)
                     /gene="rsxC"
                     /locus_tag="OO585_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6628689.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(26523..27101)
                     /gene="rsxB"
                     /locus_tag="OO585_001029"
     CDS             complement(26523..27101)
                     /gene="rsxB"
                     /locus_tag="OO585_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6628690.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVELHA"
     gene            complement(27101..27682)
                     /gene="rsxA"
                     /locus_tag="OO585_001030"
     CDS             complement(27101..27682)
                     /gene="rsxA"
                     /locus_tag="OO585_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6628691.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(27759..28199)
                     /locus_tag="OO585_001031"
     CDS             complement(27759..28199)
                     /locus_tag="OO585_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6628692.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(28285..28500)
                     /gene="ydgT"
                     /locus_tag="OO585_001032"
     CDS             complement(28285..28500)
                     /gene="ydgT"
                     /locus_tag="OO585_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6628693.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(28773..28898)
                     /gene="blr"
                     /locus_tag="OO585_001033"
     CDS             complement(28773..28898)
                     /gene="blr"
                     /locus_tag="OO585_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6628694.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29111..30151
                     /locus_tag="OO585_001034"
     CDS             29111..30151
                     /locus_tag="OO585_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6628695.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30186..31187)
                     /gene="add"
                     /locus_tag="OO585_001035"
     CDS             complement(30186..31187)
                     /gene="add"
                     /locus_tag="OO585_001035"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6628696.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31290..32462)
                     /gene="malY"
                     /locus_tag="OO585_001036"
     CDS             complement(31290..32462)
                     /gene="malY"
                     /locus_tag="OO585_001036"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6628697.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(32472..34064)
                     /gene="malX"
                     /locus_tag="OO585_001037"
     CDS             complement(32472..34064)
                     /gene="malX"
                     /locus_tag="OO585_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6628698.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34239..>34944
                     /gene="malI"
                     /locus_tag="OO585_001038"
     CDS             34239..>34944
                     /gene="malI"
                     /locus_tag="OO585_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6628699.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAIT"
BASE COUNT         8744 a         8985 c         9106 g         8109 t
ORIGIN      
        1 acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt ctgttagcgt
       61 aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa cctctggggc
      121 ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa caatttgccg
      181 ctagaaacga ccaccagcgc acccgcccga cgcgttggaa ccagcgttgc cggatgtgca
      241 ggccagggca gaagatttcc ccacacatta gccggatcgt tggcagagag cgccactggt
      301 gtatagtggc gcgtttgcgt ggcttgtgtc gccagatcgc gccatcggtc aatagtcaga
      361 cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat aattcgcccg
      421 gagtcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg gatattttcg
      481 gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc cgccagcgcc
      541 agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg tccagccaga
      601 tttggtgtgt tgtaagatac cagcggcgag acgggttgcg catagacagg acgtccacga
      661 cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg ggtgagggcg
      721 cgtaacggtg cccaaatgtc gctggtgatg acgccttgcc agaccagcgc ccataacgcc
      781 tcttgcaggg cagagagatc aaccgattcg ccgattttgt cgcgtatccg ttggctgatt
      841 tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg ttgcagcgcc
      901 gaacgattcg cctgatccgc ttcggcagga gtgaacgatt ctgcagcata ttcctgtaga
      961 tgcagtgcca ccaggccgtc atcttcaccc agtttttttt gccccgacca gataaccgca
     1021 ccggttgcca gtaattcatc gagcatttct gacgaatagt cgcgtacgcg ggcaggcagg
     1081 atctggcttt cccagagtga ggcgggtaaa ccgactccgg caagctgttc gatcacccgc
     1141 atcacgccat ctacgccctc ataaacgcct ggcgatgttg aggcaaagag cgccgggcta
     1201 ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata ggtcgtggct
     1261 gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag acgacgaaat
     1321 acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg ttcacgcagt
     1381 tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc cagttgttca
     1441 gccgtgacca gagcatgagc ccggagatag cgcagaaaga ggtcgcgaag cgggtaactg
     1501 actctatgta aataaatctc tggcaatgac tctggtagtc gtacgccgag ggcatcacgc
     1561 agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat cgctgggaag
     1621 attcgtttta ctgcaagaag attttccaga tacgacgcaa cctcttcact gctgcctgta
     1681 tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag gtcgaacaat
     1741 ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc ttccacctgg
     1801 cgaatgacct gcgggtcgag taattccccc ggatcgacct gtccgagtag attgcgcagt
     1861 aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc gtcgctctgg
     1921 tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga gggcgtagtg
     1981 gtcgttacat cggatatttg aatttcgcca ccgttcaggc gacgcatcaa acgttccagt
     2041 gcgggaagat cataaacatc ttgcagacat tcgcgtagga tttcgagaat gaccggaaaa
     2101 tctggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg ttgccaaagc
     2161 ggggtgcgat ggcccggagt gcgccccggc attaataatg cccgcgcggc gcattcgcga
     2221 aaacgggcgg cgaaaagtgc cgagctgcct accgcctcgc ggacaatttg cagcaacttt
     2281 tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc aggaatgcgc
     2341 gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag cgcccagggt tcatgcaccc
     2401 gtcttccata gggagagtgc aaaataatac gccagtcgcc aatttcatca cggcaccgtt
     2461 cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgttgc tcgtcaatca
     2521 acccctgaat attggcgttc gtattttctt ctgccagcca cgggggaata gtcccggaaa
     2581 agaacgcgcc atccgccagt aaatgaagaa aatcgccgat catctcgcct aattcagccg
     2641 gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc ggagcaggag
     2701 tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg ccgagagtga
     2761 taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagtttaccc acccgccgcg
     2821 aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga tccggaatgg
     2881 tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg gacggtcaat atcccggtct
     2941 ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga tagcgcccgg
     3001 aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac ggtgcggcac
     3061 ggcgtacgcg ggagtaccat tcgtctacct gtaatgcctc catcgccgcg gcggcaacgg
     3121 tttgctgcgc aaggacgtcg agtggattat gcggcggtgt caggttttcc agcctgcctg
     3181 cgaacataca ctctacaatg actgcggaat cgactaagtc acgccgggta cgagggaaaa
     3241 acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt tgtaacccac
     3301 tggcaacaga aagcggcgtt gccacctgaa tcaccagatc cactgcgccc atatcaatcc
     3361 ccagttcgag actggaggtt gcgaccacgc agcgtaactc acccgatttc agcgcctgct
     3421 cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca ataaaaacgt
     3481 cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg gccgcatcaa
     3541 cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat cgtgccgtca
     3601 gtttttccgc cagcccacgt gaattagtaa agacaatggt cgagcgatgg cgcaacactt
     3661 catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg gcatggctgt
     3721 cttcgccggt gctgctggcg accgatgaga catcatccat attggcgacc ggtacgacaa
     3781 ttcgtatctg cggatggcgc attgcgggcg ggttgactac cgtaaccggg cgatcgccac
     3841 caagaaaggc tgccacatcg ctggctgagc gcacagtggc agaaaggcca attcgctgtg
     3901 ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc gcaccacgtt
     3961 tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgccgcgc cgccgccgtt
     4021 catcggcaat acccttcaac gggagctgca agttgcgctg aacgtcggtg cccagagctt
     4081 ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg cgggtatctt
     4141 cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc gttttcccgg
     4201 agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc caggtttgcg
     4261 actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc gccggtgaaa
     4321 acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct attccccgtg
     4381 aatatcatgc aggatgcgtg gaatagcctc actatagatg cctgaatcat tgcatgtcat
     4441 caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc ccagacgttt
     4501 ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt acagcgcgga
     4561 gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg acaatacggt
     4621 ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag ggtggaacgg
     4681 gttacgtttc agtgaggcgc gttctgcggc ggctatcata aagctgtgat caaaattggc
     4741 attgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt tacgtacaac
     4801 tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ctacgatcgg gatcgttcgg
     4861 gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa atggttcgac
     4921 gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt tcagggtgat
     4981 ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt caacatcgat
     5041 cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag cgttatcgga
     5101 catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata ttacttgtcg
     5161 atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt tgcccagacc
     5221 gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat cttccataaa
     5281 cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt tacccccgtt
     5341 ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga tgtgaccata
     5401 agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa tcaccgcttc
     5461 ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg ggttttcgct
     5521 ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc gttgcaaatc
     5581 gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat ggctaacaga
     5641 ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt ggattacaac
     5701 gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc acgctgtggg
     5761 ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat ccagtcacga
     5821 ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt ttctactgtg
     5881 tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc gcggaacgca
     5941 tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata agcaatgccg
     6001 cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg gccgttatcc
     6061 gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc accccattct
     6121 tcaatcccgg catcgaccac ttccagtacc agacgtgcgc gattttccac gctgccgccg
     6181 tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa ataaccgtga
     6241 gcagagagga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat ggcctgacgg
     6301 aaatcattga cgatacctgg aatctcttct agttcaagcg cacgcggcat ggatgtttca
     6361 acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc gctaagtgct
     6421 gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat gcgtccggtg
     6481 tgccacagct acacggccat atgaccattt tcagcatgaa cgccagcggt gatttttttc
     6541 catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc ttttacctgg
     6601 gcagaaattt gcgtggcttc actaataatc aaaccggcac tggcacgttg gcgatagtat
     6661 tccgccatca acggggtagg aatgtcaccc ggttcaatac tgcgcagacg cgtcagcggt
     6721 gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga atacagtttt
     6781 tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta aacggcaggc
     6841 gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa actgcgcgaa
     6901 attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc ctgttgtagc
     6961 ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc ctgagagagc
     7021 agggcgatca cgccgcgagc acctttatcc atcgcgctgc gcatatcttc tgacagatcg
     7081 cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg ttggcaaaac
     7141 tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc ttcgccggat
     7201 tgcagcaact cagtcagtcg ctggtgatat gcggtgtaat gacgctcaag catggcaacg
     7261 ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac ttcagcggtt
     7321 tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag ttgctcgccc
     7381 gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg ttggagtgta
     7441 gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca gcttcaataa
     7501 tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc tttcccgtcc
     7561 agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc ggctgttttc
     7621 gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa caagaggtgc
     7681 tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca gcgtcatcag
     7741 acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc tctcatatct
     7801 gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc ccggagtttt
     7861 cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc cgccagctgg
     7921 tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat gctgatattt
     7981 atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca cctcacctgc
     8041 gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt gaagaaaacg
     8101 tcattggtca ttacatcact gaccgcgatc acatcattaa gagcgccgaa cagtcgctaa
     8161 ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac ccgttaatgg
     8221 atgccgatga agtggcggac gcgttcaaac atctgcatca gagcggcaaa gtgcgtcatt
     8281 ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt ctgccgttta
     8341 cccttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg ctggatggca
     8401 cgctcgacca actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc cttggtggtg
     8461 gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct gtggtggcag
     8521 aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta cgtttaccat
     8581 cgcagccgct gccaattatc ggttcaggta aaattgagcg cgtacgggca gctgtcgaag
     8641 cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat tcgtaaagcg gcactggggt
     8701 acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc actggtttac
     8761 acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc tggctattct
     8821 ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga tgaacctcgt
     8881 cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc attactgaaa ccgataaagg
     8941 tctggagttt tcgcccgatc tgaaagcatt accccccggt gaacatggct tccatattca
     9001 tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg cggaatccgc
     9061 aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag gtgccgggca
     9121 tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg atgccgtcat
     9181 cacgcctcgt ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg tccacgttgg
     9241 cggcgataat atgtccgatc aacctaaacc gctgggcggt ggcggtgaac gctatgcctg
     9301 tggtgtaatt aagtaattaa gaggcctgcg taccttgcgg tggtgcttgc tcaagttgcg
     9361 aaagcgagca gtacagccgc cagacaattg ccgccagctc acgggcggca ggttgatgat
     9421 gacgggcaag gctgtcgcaa atccgctgta attcttcaag tgtggccgcc agtgattttt
     9481 gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta tcccgtactc
     9541 gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca tgagaacagt
     9601 tcagcagcac tacaccccag cgtaataacc aacggcgagc cagcgcatcc tggctgttac
     9661 tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc agtgttggat
     9721 ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg cggcttttac
     9781 gagcatccga tcctggacgc agaatggcga acgctagcca agccagcgca acgccaacga
     9841 tttttgccag attatcgtta agaaagtcag caaaatcata caccggggga ttagtgacag
     9901 cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat ttgggcatct
     9961 gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa aattgccaca
    10021 gatcgctaat ctggaccatc aggccaaatt tgaccacaaa gctgaatagc gaaagtaaca
    10081 ccagcgtgcg catcagcagt gacaacgact taaacggtgc tgcgacggcg gagtagagta
    10141 cgcagctaat tgctgccagc gttaatgcat tggcaccggc atcctattgc gatgcaatac
    10201 tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac atagcttcgg
    10261 cgttgtcggt atgacgagcc agagcggatg tccgtgcgag tctggcatga tcatctacac
    10321 cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa aaataacgta
    10381 gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt agcggggcga
    10441 taatacgtgc gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc agcaattgtt
    10501 cgagaatttc tcgcgtggca cctggcggtg agggccagtt gagcaacata cgtcgcaggc
    10561 tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaagg cgcgcgtttt
    10621 gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc atggtcagta
    10681 tctgcccaat ccccccctca tgtgctgcac gaatggcatc gtttgtttca ggctgccaga
    10741 gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt aaaagggctg
    10801 tagcatcgga actgctaggc aggatcatca tcatcatgcc gccgcacaaa ataccgacta
    10861 tcacctcgca aacgcgcgcc tgagcgatat cctacagctg gctggcctca gtaatattaa
    10921 ccatcggaaa ggcgatgatg gcagccgtgt agccgccagt tgaaatgcat acgcgacgtt
    10981 attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca tgctcaatag
    11041 aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa tggctcccag
    11101 taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg tgggaaagct
    11161 aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat aataggcaac
    11221 cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt gcgccagcgt
    11281 ggccttgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta ttgacctacg
    11341 gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt atccagcgca
    11401 aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt cacatccgga
    11461 ataaggcttg agtcactttc aacgctttga tcataaatcg cccgaccgat actggaaacg
    11521 tgaccccgta acgttttgtt atcactatag agggtaattt gtgctggtgc accttcgcgg
    11581 atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg gctgtcgaca
    11641 agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt gaggttagtc
    11701 acccaaccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg tgtctgtttc
    11761 agcgtggcct gtgcggcatt aacactggcc tgcatcgctt taacattgag gttagcggta
    11821 tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt cgcttcgtta
    11881 ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt cagctcggcg
    11941 atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac cagttggttg
    12001 tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga aaccttttct
    12061 gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca cagccaccat
    12121 ccggcaagaa ccgcaactat cgctacaatg attattgaga aatacttaat tgttttaatt
    12181 gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc gcaaacagcg
    12241 ataaatccat ttacaaggga tgccagatgt caccggcgta gatccagcca cgaagcaggc
    12301 ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac ggaggaaaat
    12361 agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg agcataaact
    12421 tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa tattctttaa
    12481 gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca aatgcagcaa
    12541 tacatttgtt ttagcaataa aattgctgca cactattcta aaagccgcat aatatcttag
    12601 caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct ggcacggttg
    12661 gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt aacacaaacc
    12721 cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca aattcaactg
    12781 gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca actggaagaa
    12841 aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg tattaaactg
    12901 acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa aacccgcgcg
    12961 gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct catcgcaaaa
    13021 cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca
    13081 gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga ctgccattgc
    13141 tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc tgtaccacca
    13201 tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt ttgttcattg
    13261 cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca gtagccagag
    13321 aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca gcaccgccaa
    13381 ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt acgttaacga
    13441 tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa acatcccctg
    13501 acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa acaaccaata
    13561 cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag catccctcat
    13621 ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat atgagcaaat
    13681 cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt attgatttga
    13741 agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt
    13801 ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga aatcgggccg
    13861 ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat
    13921 agatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg tgtctctgaa
    13981 acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt ttggacagct
    14041 tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta aggaacaaaa
    14101 cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc
    14161 gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg tggcacgcac
    14221 cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag gacaaggcgc
    14281 accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc gacgaatggt
    14341 gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc cggttggcgg
    14401 ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc aggccggtaa
    14461 accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattcgcc cactgctgca
    14521 aaatatgctc agcgacccat atttctcgca acctgcaccg aaaagcaccg gacgcgaata
    14581 ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg atccccgtga
    14641 tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag ttttgttgag
    14701 cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc tactcatggc
    14761 gcgtctggcg gcattactgc caggcacaga agtcaccacc accgatgccg ttggcattag
    14821 tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc tggcgggatt
    14881 accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg gggctatttt
    14941 ccctgctaac ccgtgatatc agagttaact gaatttttct taccgcgtct gccgttacac
    15001 ttggaacgtt agggagggcg tattgccctc cagaccagga aagtcttcgg gaaatgtcta
    15061 tgaaaaaact gttactgatc tgtttgccgg tgcttctcac cggctgtagc gcgttcaatc
    15121 aactggttga gcacatgcaa accgctacgc tggaatacca gtgtgatgaa aaaccgttga
    15181 cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat caactactgc
    15241 atctcaaaca gggcatttca gcgtctggtg cacgttacac tgacggaatc tatgttttct
    15301 ggtcgaaagg cgatgaagcg actgtctata aacgcgaccg catcgtattg aataactgtc
    15361 agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag cgtcacccac
    15421 tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat cgcgcatctg
    15481 cgccgtgaat acaccaaagg cgggttacgc cgtcgcgatc ttcccgccga tccattaacc
    15541 ctttttgaac gttggctctc tcaggcgtgt gaagccaaac tggcagaccc taccgcgatg
    15601 gtggtcgcta ccgtggatga acatgctcag ccttatcaac gcatcgtttt actcaaacat
    15661 tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc acatcaaatt
    15721 gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg ccaggtgatg
    15781 gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt tcatagccgc
    15841 ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc
    15901 ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg cgaagtgcca
    15961 ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt ctggcagggc
    16021 ggtgagcatc gcctgcatga ccgctttttg taccagcgag aaaatgatgc gtggaagatt
    16081 gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt actcctgatt
    16141 ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg
    16201 cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa caattgcaag
    16261 agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga ctggcgcaag
    16321 gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat ttggggcatc
    16381 ttgttccatt gttatgcctg aaacgcttcc aacaggcggg ccacaagccg gttgcgctgg
    16441 taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag cgtaagctga
    16501 acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt gccccgttcc
    16561 tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac tggttcggca
    16621 atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt aaccagatga
    16681 tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt tcgttcactg
    16741 agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa cagtacggtg
    16801 tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt atcgacctga
    16861 cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc actaaagcag
    16921 atggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg aagaaaacca
    16981 gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt taccgcttcc
    17041 tgaagttctt cacctttatg agcattgaag agatcaacgc cctggaagaa gaagataaaa
    17101 acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact cgtctggttc
    17161 acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc agcggttctt
    17221 tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta ccgatggttg
    17281 agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg caaccttccc
    17341 gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt gaaaaacagt
    17401 ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt accttactgc
    17461 gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag tggaaagggg
    17521 gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa tattctcgct
    17581 atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt tcctatgcgc
    17641 cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca cacccagtac
    17701 ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt gcaaggcatt
    17761 gccgccattg ataaattaca cacctgtgat gccgtgttaa gtggctatct gggatcggcg
    17821 gagcagggtg aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa tccgcaggcg
    17881 aaatattttt gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt tgcaccgggt
    17941 gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg atatcattgc gccgaatctg
    18001 gttgagctgg aaatactctg tgagcatgcg gtaaataacg tcgaagaagc ggttctggca
    18061 gcgcgcgaac tcattgcgca agggccacaa attgtgttgg ttaaacacct ggcgcgagct
    18121 ggctacagcc gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc ctggcatatc
    18181 agccgtccgc tggtggattt tggtatgcgc cagccggtag gtgttggtga tgtgacgagc
    18241 ggtttactgc tggtgaaact gcttcagggg gcaacgctgc aggaggcgct ggaacatgtg
    18301 accgctgcag tctacgaaat catggtgacc accaaagcaa tgcaggaata tgagctgcaa
    18361 gtggtggctg ctcaggatcg tattgccaaa ccagaacatt acttcagcgc aacaaagctc
    18421 tgaaatatct catttcggcc cacaacgacc tgtgggccga tggctattac tttaagcctt
    18481 ccgctgacag tgcgtcttgt acttccggac gttcagccat acgttgcata aatgctgcaa
    18541 tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga accgtaaaca
    18601 gataggcatc agcaattgta aatctttgcc cgcagatcca gtgctcatcc ttcagtgtct
    18661 cgttcacata ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg tactcttccg
    18721 gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca
    18781 gccattcgat ggttttatag cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg
    18841 ggacgctgtc ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa gtaccgtcat
    18901 ccagcagcaa tgcaggcacc tgccccttag ggttaacggc aaagtaatcg tcaccgtttt
    18961 cgagacgttt tttcattaaa tccacactga cgagggtaaa atcctttccg ctctcacgca
    19021 gggtgatatg ggaagcgaga gagcaggcac ccggtttgta gaacaatttc atcggtaact
    19081 ccttttgtct gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa agccgctaac
    19141 gaaaagttag cggcttgtaa aagagtttcc ctaaatttta cgctacagca gctttcgccg
    19201 ctttgtctgc agcgtcatcc tgcgtcatac ggtgcagttt aggcgcggtc agcagcatca
    19261 gtactgcaat aatggcagta gcgacaccaa tctgcaagaa tacgcgacca taaacttcca
    19321 gtgacatcag cgggtcggta acgttatccg gcacagccat catacccgca acataaccac
    19381 caatcaggtt tgcaccggca gtggtcagga accagctacc cataatgaag cccatcagac
    19441 gctgcggaac gagttgtgca accattgcca gacccagacc agagatcatc agttccccga
    19501 tgctctgcag gccatagctt gcgaccagcc agcttacaga cacgatacca gcgtcagacg
    19561 cgaatttcgc acccagcggc agaatcagga acgcaccaga acacatcacc atgccgattg
    19621 caaacttggt tggcatcggc agggtatcgc ccatcttgtt atagatagcg gccagaatcg
    19681 gactaccgat gatgatccag aacgggttca gtgcctgata ctgctcaggt tctacggcca
    19741 gacccagaat ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac gttggcatct
    19801 ggctgtacag cacgaagaag ataatggctt cgagcatcag gatgaaggca acgatcattt
    19861 tacgacgcgc agcacctttc atcgcgaagg cttctttacc gaagataacc acgataccga
    19921 aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc caggtggcga
    19981 tagcgatcag tgccacaaca ccaataatgg tcagtagcag gttacggtag ttgataggct
    20041 cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg aagttaacga
    20101 tagtgatcag caggcctaca acgctcaacg caaacgcaac actccagccg tatttcgcgg
    20161 ccagccacgg cgtagcaatc atagagaaga aagagccgat gttgacggac atgtagtaca
    20221 tggtgaatgc accgtccaga cgcgggtcgt ttttctcata gcatgtagaa agcagagaag
    20281 acgggttagc tttaaacagg ccgttaccga ccgcaatagc cgccataccc atataaacga
    20341 taccggcgtc gtgaccagac caggcaacca gagcataacc aatcgccagc acaatagcgc
    20401 cgagcataat tacgcgttta gtacccagta ccttgtcacc taaccagccg ccgatagcga
    20461 ccagaccata aaccagggca ctaaaggaag agaaaagggt gattgaatcc gcttcagaca
    20521 tacccagttg tttaaccagg taaacagcca taattccttg taggccgtaa taaccaaaac
    20581 gttcccataa ctcaatcgag aagatgagat agaacgcctt cggttgtttg aaagcgttca
    20641 aactgacgct ttcagttggt ttttggtttg cagtggacac ttttacctct ttttttacat
    20701 cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg caattgttat
    20761 agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca agtcctttgt
    20821 aataatttgt tacatataac tgaggcgggg aattcaacca ttccgggaaa tgttatccag
    20881 cgttaaattt ttcaaaaaac gtaacggcag attttttcac tgcactgaga ggggggtgat
    20941 ggcttaacgg cgatatgttc tgctatttag cgtttttact agtgagatag tccagtttct
    21001 gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg
    21061 attttaacct gattcttgca atgtttttac attgcgacag catcgtgatc ttgatcacga
    21121 atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt tatccgcaaa
    21181 cggattacct gatgataacg ctaagcaatg gcatcattca cgttaaaaag atgttttaaa
    21241 agaggataaa gaaaggttat caatggggta atcggtgtta ccccttttct tcagatgtca
    21301 actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg gggcttgcgg
    21361 gcaatgcagg tataacgccc gtgcaggatc aaccaatggt ggcagtcgac tttaaactct
    21421 gccggaacca ctttcagtag cttttcttct acctgttcaa cgtttttccc cggcgcaaat
    21481 tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat
    21541 gcagtgttta atacgacgtt ggctgtttta cgacctacgc cgggcagggc ttcaagcgca
    21601 gcacgatctt ccggaacctc gccattatgc tgctccagta agatacggca ggttttgatg
    21661 atattttctg ctttgctgtt ataaagccca atcgttttga tataggtttt aaccccttca
    21721 acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc
    21781 ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag caattcaaaa
    21841 ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag gcgagtgagg
    21901 atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt ggcaatgcac
    21961 gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg tattttcctg
    22021 ccagcatcag tcccaggcca ataaatgcac ctggtggcag catcgccagc aggaaagggg
    22081 agtcggtgtg gaaaatctcc acgcgtaata cttttgccca gctacctaac agcgcatctg
    22141 caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag tgaacccagc acgaacatgg
    22201 cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt
    22261 tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc agcggaataa
    22321 aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc
    22381 tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt
    22441 gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc gcaagtccta
    22501 aacccagagc attagtggca gtggacgtga ccgccaacag aggacaaagg ccgagcaact
    22561 ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta atttcgctca
    22621 cgctttattc tccacaggca ggaagttgag aaagttgtgc cggtaacgtc tgagcgtaca
    22681 atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact
    22741 ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatccgca ccactgattt
    22801 ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta tcgccaagcc
    22861 ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc
    22921 ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgcttcc agaacggcgg
    22981 ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta cctaactctg
    23041 gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg gctggcagca
    23101 cctgatcaaa taatgccttt tgttgcagac ttgcctgttc agcaatcgtc gttttggtca
    23161 tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagcgct aacgtaatgc
    23221 cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag acgcgcggac
    23281 gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca aaagccacgc
    23341 cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgca agcgcgccga
    23401 aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg
    23461 cgccgagcat ggtcgcacca gacagcagat gaatttgcgg tgctgccagt gtttctggtg
    23521 agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg aggggaatat
    23581 gccagcgaat cgctttctgc catagcaacc acaggccgcc agccagccag gcgagattta
    23641 cccattgcca accagcgccc gccagaatac cgctgtagat cggatattgc ataatctgtt
    23701 caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc gcctgactaa
    23761 tgccatcaat acctaagtgt agtgtgttca tatcagcacc actggcggta tgcccgctga
    23821 aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca tgtggcggta
    23881 accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca atcattgccg
    23941 gattaaacgg gttttgcccc agaccgccat acaactgttt agcgatgatc accgcaaaca
    24001 ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc gccagcaata
    24061 agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc ttgcgtagtt
    24121 tgagtacgag agcttcggct aacagagtac taaccgatgc caacaggatc tgaacgagag
    24181 taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg agcaacacca
    24241 acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta gctattctga
    24301 ataccattta gtcctcgttt acaacctttt gctgggcggc ttttttggcc tgagcgcggg
    24361 caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt tctgcgttag
    24421 tctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg acggcggctt
    24481 tgcgcggatc aacctgttct tcaggtaccg cattagcctg ttgctgttcc agcttgcgtg
    24541 ctttggcgcg tgcaatagcc gcttcgacgg cagctttgcg cggatcaacc tgttcttctg
    24601 gctccgcatt agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca atagcggctt
    24661 cgatggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc tgttgctgtt
    24721 ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcga
    24781 cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcgcgct ttggcgcggg
    24841 cgatagctgc ttcaacggca gttttacgtg gatcagcaac ggttgctgcg tcgttagttt
    24901 gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt gctgcaataa
    24961 ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta gcctgggcct
    25021 gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg gctgcaggtt
    25081 gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct
    25141 ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga cgaatagccg
    25201 caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc gggcaaaccc
    25261 acgcgcaagc cccacattca atgcaatcag caatgttatg cgtggtagct ttatcgtgtt
    25321 gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg caggcgtcag
    25381 cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagt tcattggcag
    25441 agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat ggcaaggtaa
    25501 agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag aatcgggcat
    25561 cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc gggcgagcga
    25621 ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca ataacggcac
    25681 gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg gatgaacgcc
    25741 cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag
    25801 gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc accgcgcgca
    25861 gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg cgtggctgca
    25921 gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc tgcatcaaac
    25981 ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac gtttcaatct
    26041 tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc agcccggcaa
    26101 caccaaactg atgtatgcgc tcgattaact cttcgcgact acgagtgcga taatcggccc
    26161 agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg cttaattcag
    26221 ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg
    26281 gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg cgcaatactt
    26341 tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc agtggaataa
    26401 caaaacgctg cgccagtggt acctggcgca ggggtgtacc gttggactgg gttttcatct
    26461 ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat gcagagaata
    26521 acttaagcat ggagttccac gggaatgata cgcacgggaa tggtgttcag atcccatttc
    26581 caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg gcacggatca
    26641 acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc acgggtagcg
    26701 ccaacgatgg cgtctaccgg acaagcctga atacatttag tgcagccaat acagttattt
    26761 tcatcaataa ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc atccagcggc
    26821 tgcggctcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc acctggggcg
    26881 caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg acagccggga
    26941 taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc aacgaccgga
    27001 tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa cgccaggccc
    27061 agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc accaaaccac
    27121 taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg gcattaccgc
    27181 gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggtg aagagcacca
    27241 tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc aagaaattgt
    27301 gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg gtgataagcg
    27361 gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc accatctcgg
    27421 tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg taaatcagat
    27481 taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc agcgtcatca
    27541 caaacgttgt tgccagcccc atgcccatcg cggtttccag ttttttggaa acccccataa
    27601 acggacagag accgagaaac ttgaccagca caaagttatt gaccagtaca gttccgacaa
    27661 acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct attatccgtt
    27721 aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac aaacgtcgct
    27781 tttacccgcg acgagcgttt gaaataggag acgatcaatg cggttgccaa caaagtaaac
    27841 agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaatgc ttttactgcc
    27901 agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg ccgacgttta
    27961 aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt aataaaagaa
    28021 acgccccata agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa tgtttgcgga
    28081 gaagataacg cagcggtata cagcaacaac gccagcgtcg tactcaacag agcgactaaa
    28141 agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt ggtggtcata
    28201 ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt attataaggc
    28261 ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact tcggtacctg
    28321 gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat cggcagcacg
    28381 atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt cgtagagttt
    28441 ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct gaacagtcat
    28501 aaaaggtcca taaaataatt aataccaaag gggtaggtat tattagtgat gataaaattt
    28561 caccaaaagt gaacgcttgt gttattacaa taataagaat gcgctgatgg caaatattca
    28621 gggatgaaaa actgctctga aaagagtaac gcgctttact atccctgaca atcaccaaca
    28681 acatcgaaca agataataaa ttcctggttt aatatccgac aagtgaaaac atgcaaccgg
    28741 acgggcagca tggcgctcta caagcgcaga gcttacttgt gttgtaccga agcactctgt
    28801 tcaggtggct gatagttgtc aatgtgactc gccacgccaa gaagaatgac tgaaacgaca
    28861 agaacgatcc aacctgttaa ttcaataaga cgattcatta cagcccacat ctctcttgat
    28921 tgatccatta acttcagggg gtaaatgtta cttagcaata atagctccag caagattttt
    28981 actgaggttt ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt ttttgactta
    29041 atattaaact gttgagcgtg ggtaaaggat agtgtcaaat agcaatcata cttcaatgag
    29101 aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt atgcgagcaa
    29161 aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg taatctccag
    29221 cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct ctgagccgaa
    29281 gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca acgataccca
    29341 tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg ataaaccctt
    29401 taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc tggggcgtgt
    29461 gctgtctgta ttccataacc gtcgttggga tagcgatttc ttgacgctaa aaggtttgct
    29521 cgcggaaggc gtgctgggtg aagttgctta ctttgagtct cattttgacc gcttccgtcc
    29581 gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct ggtacgattt
    29641 agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca gcatgacggt
    29701 agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg ccatcttgtc
    29761 ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg agtcagcacg
    29821 gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc cacaggaaga
    29881 acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata tgcgtgatgg
    29941 cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga cggtacctgg
    30001 gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg gtgaaaatcc
    30061 ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca tcgaatccgc
    30121 caaacatcgc gcgactttgt gccttgcatg aaaaatggat ggtgcaattt gcaccatcca
    30181 ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt tttcctcagc gctgaggaaa
    30241 gccatttcca gaccattaat ctgtgcctgg cggatttgct cgcgggataa cccagcagct
    30301 ggcgcggcaa cggtatattc gtgaatgata tccactccct gtacgccggg atcgtcagtg
    30361 ttaatgccgg cacgaatgcc atgctcaagg aacgttttca gcggatgtgc agccagctct
    30421 gctacggtgc tggtctgaat attggaggtc agacaggatt caataccaat ttgttgctcg
    30481 gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc aatacgctcc
    30541 gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc gcctgcatgg
    30601 acggtaatat gccagcccgc atcacgcgca aggttgaagt gagaaaggaa cagacttccc
    30661 gggaaaccaa gttcatcacc ggctaaatca agggcggtaa tctggtcacg gtgggctaaa
    30721 aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat aatgccgata
    30781 agcttcgcct gcacaccaaa agtgcggcaa ccttcacgta cgccatcgat caccgcttcg
    30841 acaacacccg ctacaggcag cttatgtgcc attgccatgt agcctggtga aaaacgcagc
    30901 tcgacatagt gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc cacgcggcgg
    30961 caggcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag aaagctcacc
    31021 agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttccag ggattgtgca
    31081 ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg aatgttgcca
    31141 tcaaggtggc gatggatatc agttaatggc agggtggtat caatcatggt cgcactcttt
    31201 ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat ccacgattgc
    31261 gcaacgattt ttacgttgcg caattggggt taacgaacag cgcggatggc gttaattaat
    31321 ccagccacac ctttttccag tttcgaacgt gggcagccgg cattgagacg gacaaaacca
    31381 cgaccttctt caccgtaggt ataccccggc atgatcgcga ctttttcttg ttcgataagt
    31441 gctttttgca acgcgttgtc gtcaatattc aacggacgta aatcaagcca tgccagataa
    31501 gtggattgtg ggatctgcca gttgagttca ggaaacgcgg cgttcatttt atctgcgata
    31561 tacgtcaggt tatctttcag atagatgcgt aaggcatcca gccacggcgc gccttgctga
    31621 taggcggcga tatgggcagt taacgccagt accgaagggg aagaaagccc atcacggcct
    31681 ttcagtgccg ataaataggc atcgcggcta ctgctatttt ctataatccc gtaagcaccg
    31741 gtcagggcgg gaatattgaa acttttcgag cccgacgtta gcaacgccca gtctccgcga
    31801 gccacattac tccagggaat atgcggctgc tcgccccaaa ccatatccat atggatttca
    31861 tcggaaataa cccgcacacc atgacgctcg cacaggtcag ccatgatctc cagctcatcg
    31921 cacgtccaca ctttcccggt aggattctgt gggctacaca ggagcataat tttacattct
    31981 ggtttcgcca acacggcttc caacttgccc atatcgcaaa accaaccatc agcctgcttc
    32041 tctaaagcaa cgggcattac tgtgcgctgg ttaccttcaa tggccttgta aaatgcgtca
    32101 taggcgggtg tgtggatcac cacgccttca cctgtttcag accactgacg aatcagttct
    32161 gaaaccatat agatgacaga agggccatac accaccgtct gagaatcgat ggcggtgtaa
    32221 tgctgggtgg aaaaccagtg ggcaatagca gcgagaaact catcgttttt ccagcggctg
    32281 tagccaaata cgccgtgcat caggcgctga ttcagcgcct ctataatgca gggggcagtg
    32341 gcaaaatcca tgtctgaaat cgtgaacggt aacaggtcag cagtgccgaa acggtcagcg
    32401 acataatccc actgtgtaca ccatgtgcca tgacgatcca cgacctttga aaaatcgaac
    32461 atatcttatc cttatgcctg gacagtatgc atcagaccgg ccatttcatc ttttactgac
    32521 tgaacttgtg gcccgataac aacctgcagg ttatgttgat taagttgtac tacgccaatt
    32581 gcccgattgt ccttcagtgc ctgcacatta acaagcgaca tatctttcac agacaaacgc
    32641 agacgggtaa tgcagttatc gaggctgaca atattgtcgg caccgcctaa tgcttcgagg
    32701 attgcaggaa cgttgtaacc tgatttaccc ggcgcagcgg caacggcttt ttcgattgag
    32761 ctggcaactt cgctatcgcg ccccggggtt ttgagattga agcgggtgat agcgaaacgg
    32821 aagatggcgt agtaaacgac aaaccagatt gccgccacca ctggcaccat gtaccacttg
    32881 gttgacagac catgcaaaat accgaacacc acgaagtcga tgatattgcc gtcggtatta
    32941 ccgatggtga cgccgagcac agacatgacg gtgaagccga ggccggttaa cagcgcgtgg
    33001 atgacataca gaactggcgc tacgaacagg aacaggaatt ccagcggttc ggtagtgccg
    33061 ccaacgacgc aggcgatcag gccagaaatc agcagacctt taattttatg gcgattttcc
    33121 gggcgcgcgc agtgatacat agctaacgct gcagctggca gaccgccgag aaacgcaggc
    33181 attttacctt gcgaaaggaa acgcgtggcg ctttcagaaa aaccgtgagt ggtcgggcaa
    33241 ctcaattgcg tctggaagat ggtcagtgcg ccgctgacgg tttgaccgca gacttcctgc
    33301 gtgccgcctg cgtcggtaaa gcgaattaat gccaccagaa tgtgatgcag accaaacggc
    33361 aacagcagac gttcaccggt accaaacagc atcggtccga aatcacccgc gctgtttatc
    33421 atatggccca agccgctaat acccatggcg aaaatcggcc agactaatgg aatcaccagg
    33481 ccgacaaggc ccatcaccag cgaggagata attggtacga agcgcgtacc gccgaagaat
    33541 gccagcgcat ccggcaggcg gatattatgg aaacgctcat gcagcatcca gacgataata
    33601 ccggcgatca ccgcaccgag gatcccggta tcgatcgact ggatcccaag aatgctctgg
    33661 atgttattgg ctttcagaac cgcggcatcc gtggttggca gaatgccttt attggtcaac
    33721 cagaagttta ccgcgaggtt cattaccgca taaccgacga agccagcgaa tgccgctacg
    33781 cctttatttt cgcgtgccag gcccagcggg atggcgatac agaacatgac aggcaggaaa
    33841 ctaaaagcaa acgagccaat cttactcatc caggtaaaga tagcctgcaa cacggggttg
    33901 cccaggaccg ggatcagggt tatgacatca tggctgctaa gagaactacc aatgccgagc
    33961 ataatgccgc agaacgacaa taatgccacg ggtaacatga aggttttgcc taactgctgg
    34021 aagaactccc acagcgtgac tttcggtgct gttttcgccg tcataaaacg actcctcgta
    34081 gagaacaatc tggattcagt aaattgcgcg agaagataaa acgttttatc aaattttagt
    34141 gaggcataaa tcacattacg caacgataat agcgggtata agataaataa aaggtaaaac
    34201 gttttatctg tcacataatc agggagtagg tcatctgcat ggctaccgcc aaaaaaataa
    34261 ccattcatga tgttgcgctg gctgcgggcg tgtcggtaag taccgtttcg ctggtgctta
    34321 gtggcaaagg gcgaatctct accgccacag gagaacgcgt taacgccgcc attgaagagc
    34381 tgggatttgt gcgcaatcgc caggcgtcgg cgctgcgcgg cgggcaaagc ggcgtcattg
    34441 gtttgatcgt ccgtgattta tctgcgccgt tttacgccga attgacggcc ggattgacgg
    34501 aagctctgga agcgcaggga cggatggttt ttttgcttca cggcggtaaa gacggcgagc
    34561 agctggcaca gcggttttca ctgttactga atcagggggt cgatggtgtg gtaattgccg
    34621 gggctgcagg aagcagcgat gacctgcgac ggatggcaga agaaaaagct atcccggtga
    34681 ttttcgcttc ccgtgccagt tatcttgatg atgttgatac ggttcgcccg gacaacatgc
    34741 aggctgcaca gttgttgacg gagcatctca ttcgcaatgg gcatcagcgg atcgcctggc
    34801 tgggagggca aagttcctca ttaacccggg cagaacgggt gggaggctat tgtgcaactc
    34861 tactaaaatt tggtctgccg tttcacagcg attgggtgct ggagtgcaca tccagccaga
    34921 agcaagctgc ggaagctatc acgg
//
LOCUS       DAJTYD010000024        34799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTYD010000024 DAJTYD010000000
VERSION     DAJTYD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.024"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..334)
                     /locus_tag="OO585_001039"
     CDS             complement(<1..334)
                     /locus_tag="OO585_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6628700.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OO585_001040"
     CDS             complement(415..1842)
                     /locus_tag="OO585_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6628701.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OO585_001041"
     CDS             complement(1839..2546)
                     /locus_tag="OO585_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6628702.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="OO585_001042"
     CDS             2709..3686
                     /locus_tag="OO585_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6628703.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OO585_001043"
     CDS             complement(3756..4238)
                     /locus_tag="OO585_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6628704.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OO585_001044"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OO585_001044"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6628705.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OO585_001045"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OO585_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6628706.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OO585_001046"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OO585_001046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6628707.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OO585_001047"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OO585_001047"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6628708.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9957
                     /locus_tag="OO585_001048"
     CDS             9349..9957
                     /locus_tag="OO585_001048"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6628709.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPPLTARGIEQAQNLHTLLHGV
                     SFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENY
                     SAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLI
                     GMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10217..10534
                     /gene="rsfS"
                     /locus_tag="OO585_001049"
                     /gene_synonym="rsfA"
     CDS             10217..10534
                     /gene="rsfS"
                     /locus_tag="OO585_001049"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6628710.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10538..11005
                     /gene="rlmH"
                     /locus_tag="OO585_001050"
     CDS             10538..11005
                     /gene="rlmH"
                     /locus_tag="OO585_001050"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6628711.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11036..12937
                     /gene="mrdA"
                     /locus_tag="OO585_001051"
     CDS             11036..12937
                     /gene="mrdA"
                     /locus_tag="OO585_001051"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6628712.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12940..14052
                     /gene="mrdB"
                     /locus_tag="OO585_001052"
                     /gene_synonym="rodA"
     CDS             12940..14052
                     /gene="mrdB"
                     /locus_tag="OO585_001052"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6628713.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14063..15151
                     /gene="rlpA"
                     /locus_tag="OO585_001053"
     CDS             14063..15151
                     /gene="rlpA"
                     /locus_tag="OO585_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6628714.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15291..16502
                     /gene="dacA"
                     /locus_tag="OO585_001054"
     CDS             15291..16502
                     /gene="dacA"
                     /locus_tag="OO585_001054"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6628715.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16613..16876
                     /locus_tag="OO585_001055"
     CDS             16613..16876
                     /locus_tag="OO585_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6628716.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16977..17618
                     /gene="lipB"
                     /locus_tag="OO585_001056"
     CDS             16977..17618
                     /gene="lipB"
                     /locus_tag="OO585_001056"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6628717.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17877..18830
                     /locus_tag="OO585_001057"
     CDS             17877..18830
                     /locus_tag="OO585_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6628718.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19039..20004
                     /gene="lipA"
                     /locus_tag="OO585_001058"
     CDS             19039..20004
                     /gene="lipA"
                     /locus_tag="OO585_001058"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6628719.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO585_001059"
     CDS             complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO585_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6628720.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20437..21225)
                     /locus_tag="OO585_001060"
     CDS             complement(20437..21225)
                     /locus_tag="OO585_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6628721.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENAVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21318..21701
                     /gene="crcB"
                     /locus_tag="OO585_001061"
     CDS             21318..21701
                     /gene="crcB"
                     /locus_tag="OO585_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6628722.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO585_001062"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO585_001062"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6628723.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO585_001063"
     CDS             complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO585_001063"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6628724.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23289..24674
                     /gene="dcuC"
                     /locus_tag="OO585_001064"
     CDS             23289..24674
                     /gene="dcuC"
                     /locus_tag="OO585_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6628725.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO585_001065"
                     /gene_synonym="citB"
     CDS             complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO585_001065"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6628726.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO585_001066"
                     /pseudo
     CDS             complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO585_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27370..28428
                     /gene="citC"
                     /locus_tag="OO585_001067"
     CDS             27370..28428
                     /gene="citC"
                     /locus_tag="OO585_001067"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6628727.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28443..28739
                     /gene="citD"
                     /locus_tag="OO585_001068"
     CDS             28443..28739
                     /gene="citD"
                     /locus_tag="OO585_001068"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6628728.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28736..29644
                     /gene="citE"
                     /locus_tag="OO585_001069"
     CDS             28736..29644
                     /gene="citE"
                     /locus_tag="OO585_001069"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6628729.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29655..31187
                     /gene="citF"
                     /locus_tag="OO585_001070"
     CDS             29655..31187
                     /gene="citF"
                     /locus_tag="OO585_001070"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6628730.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPTR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31191..31741
                     /gene="citX"
                     /locus_tag="OO585_001071"
                     /pseudo
     CDS             31191..31741
                     /gene="citX"
                     /locus_tag="OO585_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31716..32593
                     /gene="citG"
                     /locus_tag="OO585_001072"
                     /pseudo
     CDS             31716..32593
                     /gene="citG"
                     /locus_tag="OO585_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32644..34107
                     /gene="citT"
                     /locus_tag="OO585_001073"
     CDS             32644..34107
                     /gene="citT"
                     /locus_tag="OO585_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6628731.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34218..>34799
                     /locus_tag="OO585_001074"
     CDS             34218..>34799
                     /locus_tag="OO585_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6628732.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKT"
BASE COUNT         8653 a         8873 c         8612 g         8661 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcagctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgctttc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt
     9481 tcctttgatc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
     9541 ctcagtgacc gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc
     9601 gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg
     9661 tggtgcaatg actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg
     9721 caacgtgtgg aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta
     9781 gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc
     9841 gaggctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
     9901 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgataa tgcatgactt
     9961 ttctgttttt ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc
    10021 tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc
    10081 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
    10141 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
    10201 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
    10261 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga
    10321 ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt
    10381 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
    10441 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
    10501 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
    10561 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
    10621 gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga
    10681 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
    10741 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
    10801 ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt
    10861 gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc
    10921 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
    10981 caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa
    11041 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
    11101 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
    11161 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
    11221 gcctatcgcg cccagccgcg gcattattta cgaccgtaat ggtatccctc tggccctcaa
    11281 ccgcactatc taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct
    11341 ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga
    11401 gcgcgcacgt tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca
    11461 agtagctcgc tttgccgtca atcagtaccg ttttccgggg gttgaagtta aaggctataa
    11521 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
    11581 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
    11641 gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
    11701 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
    11761 accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
    11821 tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg
    11881 ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ttgtttgttg acggtatctc
    11941 cagcaaagat tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac
    12001 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag
    12061 cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg
    12121 ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
    12181 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
    12241 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
    12301 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
    12361 gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc
    12421 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
    12481 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
    12541 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
    12601 tgctaaccgc cctaacggta cgacgcataa atactttgcc agcgcaccgt acaaaattgc
    12661 ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
    12721 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    12781 tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac
    12841 gctgatgcgt cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    12901 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    12961 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg
    13021 ctggtttaca gcgccctagt tatctggagc gccagcggtc aggatattgg catgatggag
    13081 cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    13141 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    13201 gcggtagatg ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt
    13261 gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt
    13321 atcaaccgcg acgtttgccc accgtcgttg aagaacacag gcatcgcgct ggtgctgata
    13381 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    13441 ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    13501 gtagtgctgg tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag
    13561 cgccagcgcg taatgatgct cctagacccg gaatcagacc cactcggcgc gggctatcac
    13621 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat
    13681 ggcactcagt cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta
    13741 ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg
    13801 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    13861 ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    13921 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt
    13981 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    14041 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    14101 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    14161 tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact
    14221 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    14281 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa
    14341 cctgacggcc tctggcgaag ctttcgatcc gacacagctg acggcggccc atccaacgct
    14401 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    14461 cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc
    14521 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    14581 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    14641 cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca
    14701 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    14761 ggtaaccagt agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg
    14821 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac
    14881 ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    14941 gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    15001 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    15061 ccccttcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    15121 attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    15181 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    15241 tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15301 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15361 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15421 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15481 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15541 gccaagcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15601 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15661 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15721 gtgtcgccat ggctgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15781 gctacgttaa cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg
    15841 ctgatggtca gtacagctcc gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg
    15901 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    15961 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16021 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16081 tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16141 tgctgacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16201 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16261 acatgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16321 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16381 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16441 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16501 aaaaattaaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16561 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16621 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16681 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16741 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16801 cgccactcat atcgagcagg ttgaaatact gtatgaagaa ctgggcaaaa tcgatattgt
    16861 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
    16921 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    16981 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg
    17041 ctatgcatga attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg
    17101 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17161 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17221 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17281 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17341 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17401 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17461 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17521 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17581 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17641 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17701 attttcttta tcacttgcag aaatgaggtg ccacgccttg ctccgtaact caccccattg
    17761 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17821 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    17881 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    17941 tatttacaac cttacgaaat attgctctta accttctgac tatttttgaa gctgtatatg
    18001 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gcccccctcg gcaatcagtc
    18061 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccaag
    18121 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18181 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18241 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18301 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18361 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18421 atgttctgtt caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt
    18481 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc
    18541 aaaatttcag cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt
    18601 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18661 ttattcccag ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt
    18721 ttccgtcctt aaataaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18781 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    18841 ttacagccag atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt
    18901 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    18961 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    19021 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    19081 gatgccgata agatggccct tatcccggtt aaaaacgtgg caatagagcg cgaagccctg
    19141 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    19201 atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    19261 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    19321 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    19381 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    19441 tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact
    19501 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt
    19561 cgtatggatc gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac
    19621 ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg
    19681 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtcta
    19741 atggtgggac tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    19801 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    19861 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    19921 ggctttaccc atgctgcatg cggtccgttt gtccgttctt cttaccacgc cgatttgcag
    19981 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    20041 cccgcttttt gaagcggttt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    20101 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    20161 ccgcagcatc atcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    20221 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacta
    20281 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    20341 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    20401 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    20461 gcgacggttg tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    20521 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc
    20581 tataatccgg ctttgcccga tatttttgtt cccgcactcg cccgccgcca ccatataaca
    20641 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    20701 gcgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc
    20761 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc
    20821 cagtaacgga gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc
    20881 atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag
    20941 catattccat gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat
    21001 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    21061 tttaaccgat agatctgcat catgatcatc gcgtgccagc aatgcttccg gcaggacaaa
    21121 cagcgatacg gcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    21181 ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca
    21241 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    21301 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    21361 gaagcgtggc gagatggctg ttaagtatgc gatttaaccc actgcatcag gcgattccgt
    21421 tggggacgct gacagcaaac ctgattgggg cattcatcat aggaatggga ttcgcatggt
    21481 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    21541 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggtc
    21601 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgacag
    21661 cactggcttt ctggctgttt tcggcctcaa ccgcacacta aagacccaaa aaaaacccgc
    21721 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21781 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21841 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    21901 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    21961 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgcgtcggt taccagtaca
    22021 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22081 ttttgatgtt acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct
    22141 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22201 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22261 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22321 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22381 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22441 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22501 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22561 tgttcaggct gttgccaggt tggtgcaata ttttctctaa acgttgtcat ccactcatct
    22621 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22681 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22741 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22801 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22861 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    22921 atgcaatcaa ggaaaaatta ttttattttt taacgagata caacaatcac cttaacaaag
    22981 tatataatat atatcgtcaa cctatgctca tattagatta ttttttgagc gcacagccgc
    23041 agctaacacc attgcaatta acaaatttgc atcaatccac catcaatttg cacacattat
    23101 tatgtgataa ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagtaac
    23161 ttcaatgtct taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg
    23221 gcatattttt tccttaagct ttaggaattt tttattattt actttggggc ctggagacag
    23281 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23341 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23401 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23461 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23521 acctcggcat gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg
    23581 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23641 tgctgatgat tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa
    23701 ccggtctggg tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca
    23761 gtcgtggcgc agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23821 caggggatgt ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca
    23881 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    23941 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24001 tcaccaccac tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24061 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24121 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24181 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24241 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24301 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24361 caatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24421 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24481 tgcaggcgtc aaaccttggc cgtacccttt cgcccgtttc tggcgtagtc gttgcggttg
    24541 ccgggatggc gaagatctcg ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg
    24601 ttggtttggt gattgttatc gttgctacag agctgatggt gccaggaaca gcagcagcgg
    24661 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24721 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24781 atggcggctg gcgcaatatt caagataacg tctggcagac gtgcggctga tggtcagtgc
    24841 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    24901 gtttagcgtc aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata
    24961 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25021 tttacgctgg cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat
    25081 gagataatca aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc
    25141 agtggtaaac accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat
    25201 ccctctgccg tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat
    25261 catcattcgg gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg
    25321 acgaatatat tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt
    25381 taatggagct gtcattaggt ttcactttcg gaatatagat tgaaaataag gtaccgcagg
    25441 gatcattatc ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt
    25501 acaacccaat gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata
    25561 ttttgtctcg tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat
    25621 catcgccttc atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc
    25681 tggcttcgaa ggcgttatca agtaaattgc ccgcaatggc tgcaaactcg gtgctatcca
    25741 gtcctggcgg cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt
    25801 cccgggcgcg ctgcacttta ccaaaaagca gccccgccac ctagcgatcg gcaaacgcct
    25861 cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca
    25921 cgcgatcata ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct
    25981 cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat ttgcgtcagt tgcgcattga
    26041 gggtggaaat ttcgtcttta ctgcgaaagc tgataatggc ccccagcaaa tcatcacctg
    26101 aacgaatagc ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accatatcct
    26161 gacgtttttc atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg
    26221 gtttacccaa ccattgccgt ccgggggaac tcagccccag catctttctt gcgttacgat
    26281 tgatggcggt aatgtaaccg tgcggatcca ccgcaatcag cccttcataa accgaactaa
    26341 acagcgcctc ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcatct
    26401 gccgacggat atgcgcggcc aggaaccacg acagcaacat cagaatccct aacagcacga
    26461 caaacacacc tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca
    26521 ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg
    26581 cgcgcatcgc catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca
    26641 gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa
    26701 ggcggatcga gtgccggtcg ccatcaccac ataatcaaaa tcggtatctc tttgtaattt
    26761 gttagcgatg gtcgccagcc gtttgtagtc acgcgttttc accgcagaga tgacactgtc
    26821 attggaggca ataattttcg cctgattcat tgccatgtcg cgtacatgaa gcgttaaata
    26881 gtcctcaaaa ctggccgtaa aatattgcgc caaggctgca atgacaaata ttgagaacac
    26941 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
    27001 attctcgtta agctgcaaca ttgttttcct tgcgggttat cccgagcctc tttgacagat
    27061 gtaattaaaa tgttgaatca aaatacatag atattaatta tgatcgtggc aaattttatt
    27121 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
    27181 acttaaacca ttaaataaat aaaaatttca cgccgaaata ctccttagga tgtatagcga
    27241 aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat aaaaggttat
    27301 cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac cttcaggata
    27361 ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga aaataaaaaa
    27421 atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa
    27481 gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat
    27541 attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta
    27601 gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc
    27661 aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac cagcgtaccc
    27721 ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg
    27781 aaaaaatttc gtcatccagg gaacaagatt ggctgcattg tgatgaacgc caatcccttt
    27841 acgaatggtc accgttatct gattcaacaa gctgcggcac agtgcgactg gttgcatctg
    27901 tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga tctggtgtta
    27961 aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc
    28021 cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc
    28081 gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cactgggtgt aactcaccgc
    28141 tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac
    28201 tggctgaaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat tgagcggctg
    28261 cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat
    28321 ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt
    28381 gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa
    28441 aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac
    28501 gcatcgcccc actcgatacg caggatatcg acctgcaaat caacagcagc gttgagaaac
    28561 agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg
    28621 gcgtacagct gaatgtcgat gacaaaggca cactggactg cattttacgt gcacgactgg
    28681 aagccctgct ggcacgcgcc agcggtatcc cgactctgcc atgggaggat tgccaatgat
    28741 ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg
    28801 tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga
    28861 cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc
    28921 gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg cgctggattc
    28981 cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct
    29041 gccgaaaacc gataccgctc aggatgttct ggatattgaa aaagagatcc tgcgtatcga
    29101 aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct
    29161 gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct
    29221 cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact
    29281 gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga
    29341 taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa
    29401 acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa
    29461 cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga
    29521 agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc
    29581 ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga
    29641 ataaggcaat caaaatgacg cagaaaattg aacaatctca acggcaagaa cgggtagcgg
    29701 cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca aagcaaacct
    29761 accaggctga aaaagcgcgc gatcgcaaac tgtgtgccaa cctggaagaa gcgattcatc
    29821 gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc
    29881 tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgactctgg
    29941 cctccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg
    30001 ttacccgcat ttatacctct ggcctgcgcg gtccactggc ggaagaaatc tcccgtggtc
    30061 tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg gtacagagcg
    30121 gcgaactgaa tatcgacgtg gctttcctcg gcgtcccatc ctgtgatgaa ttcggtaatg
    30181 ccaacggtta caccggtaaa gcctgctgtg gctccctcgg ctatgcaatg gttgatgccg
    30241 acaacgcaaa acaggtcgtg atgcttaccg aagaactgct gccttatccg cataatccgg
    30301 caagcattga gcaagagcag gttgatttga tcgtcaaagt tgatcgcgtt ggcgatgctg
    30361 caaaaatcgg cgctggtgcg acccgtatga ccactaaccc gcgcgaactg cttattgccc
    30421 gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa agaaggtttc tccatgcaaa
    30481 ccggcaccgg cggcgcttcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc
    30541 gcgatattcg cgccgacttc gccctcggcg gtattactgc aacgatggtt gatctgcacg
    30601 aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt tgacagccat gctgcgcaat
    30661 cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt
    30721 cgaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca
    30781 cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg
    30841 gtcactgcga taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg
    30901 gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg
    30961 atattctggt cacagaccac ggtatcgcag ttaacccgac acgtccggag ctggcagaac
    31021 gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgtgaa cgtgcgcgtc
    31081 tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt
    31141 accgcgatgg ctcggtgatc gatgttgtac atcaggtgaa ggaataagcc atgcacctgc
    31201 ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg
    31261 aaaggcaagc acggcaacac gtctggctca agcgccatcc tgttccactg gtctccttta
    31321 ccgtggttgc gcctaggccg attaaagaca gtgaggtcac acgccgaatt tttaatcatg
    31381 gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg
    31441 cactggtttc cgccagcggg ccggagggta tgttgagcat tgccgccccg gctcgcgacc
    31501 tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg
    31561 atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc
    31621 gctgcctgtt gtgcgagcaa agcgcagccg tctgcgcgcg tggaaaaccc atcaactgac
    31681 cgatttactc aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta
    31741 aaaccacaaa gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcacgcca
    31801 tgctgactga agtcaatctg tcaccgaaac caggtctcgt ggatcgcatt aactgcggtg
    31861 cgcataaaga tatggcgctg gaagatttcc accgcagcgc gctggcgatt cagggctggc
    31921 taccccgttt cattgaattt ggtgcctgta gtgcggaaat ggcaccagaa gcggtactcc
    31981 acggattacg cccaattggt atggcttgcg aaggtgatat gttccgcgcc accgcgggcg
    32041 taaacacgca taagggcagc attttttctt tagggctact gtgtgcggca attggccgtt
    32101 tgcttcagct caaccagtcg ctaacgccaa caaccgtttg ttctacggcg gcaagtttct
    32161 gccgtggcct gaccgatcgc gaactgcgta ccaataattc acaactgacg gcaggtcaac
    32221 ggttgtacca gcagcttgcc cttaccggcg cacgcggtga agccgaagcg ggttatccac
    32281 tggtgatcaa ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg
    32341 aactggcatt gctcgatacc ttgctcctac tgatggcgat caacggcgat accaacgttg
    32401 cttcgcgcgg tggcgagggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca
    32461 aaaagggggc attcgaaccc ccgccgatct cgattatctc cggcagttcg acagggagtg
    32521 tatcgaacga aatctcagtc taggcggcag tgccgacctg ctgatcctta cctggttttt
    32581 agcacagatt taattattta agcacttgat aaatttggaa atattaattt tcggagaacc
    32641 cgtatgtctt tagcaaaaga taatatatgg aaactattgg ccccactggt ggtgatgggt
    32701 gtcatgtttc ttatccctgt ccccaacggt atgccaccac aggcatggca ttacttcgct
    32761 gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac agcgatcagt
    32821 tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa
    32881 ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct ggctggtttt
    32941 tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt
    33001 tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg
    33061 acgttgggtt atgcgattgt cattatcgac attctgctgg cgccgtttac accgtccaac
    33121 accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa
    33181 tcattcccca acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc
    33241 attagtacca gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt
    33301 ctggagttcg tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc
    33361 ttcctgccgg ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac
    33421 aaaccggaaa tcacacacag tgaagaagtg gcaacctggg cgggtgatga actgaaaacc
    33481 atgggtgcgc tgacacgcag agagtggaca ctgattggcc ttgtattgct cagcttaggt
    33541 ttgtgggttt ttggcagtga agtcattaat gctactgcag ttggtctgct ggcagtttcg
    33601 ctaatgctgg ccctgcacgt tgtgccttgg aaagatatta cccgctataa cagcgcatgg
    33661 aacacgctgg tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggc
    33721 tttattgact ggttcgccgg taccatgagt acgcacctgg aaggattctc accaaacgca
    33781 acggtgattg tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct
    33841 gcgcacaccg caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta
    33901 ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg
    33961 ccctatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat
    34021 tactggcgtc ttggcgcaat cttcggggtg atttatatct ctatgttgct gttggttggc
    34081 tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc
    34141 ccgccaggta agcccacctg gcgggcgtga tgatttaata gatagtattc agtgtatgta
    34201 tgagttccac acccattatg aaagcattct ggcgtaacgc cgcgttgctc gcggtttctc
    34261 tgcttccctt ctcttctgcc aacgccgtag cgttgcaggc aaaacagtat ggcgattttg
    34321 atcgctatgt cctggccctc tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa
    34381 atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac gaccaacaaa gctgattttc
    34441 tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg
    34501 aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat cccgaatcta ccagaagcgc
    34561 acgccagccg gatgtgttca tcgccggaaa ccgggttatc actggaaacg gccgctaaac
    34621 taagtgaagt catgccagga gctggcggac gttcctgcct ggaacgctac gaatatgcca
    34681 aacacggtgc ctgctttggt tttgatccgg acgcatactt cggtacgatg gtacgcctga
    34741 atcaagaaat taaagagagc gaagccggaa aattccttgc ggataattac ggtaaaaca
//
LOCUS       DAJTYD010000025        34569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTYD010000025 DAJTYD010000000
VERSION     DAJTYD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.025"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..940)
                     /gene="istA"
                     /locus_tag="OO585_001075"
                     /pseudo
     CDS             complement(1..940)
                     /gene="istA"
                     /locus_tag="OO585_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1556..1693
                     /locus_tag="OO585_001076"
     CDS             1556..1693
                     /locus_tag="OO585_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628733.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            2293..3072
                     /gene="ipaJ"
                     /locus_tag="OO585_001077"
     CDS             2293..3072
                     /gene="ipaJ"
                     /locus_tag="OO585_001077"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6628734.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(4016..4945)
                     /gene="virB"
                     /locus_tag="OO585_001078"
     CDS             complement(4016..4945)
                     /gene="virB"
                     /locus_tag="OO585_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6628735.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(5162..5398)
                     /locus_tag="OO585_001079"
     CDS             complement(5162..5398)
                     /locus_tag="OO585_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6628736.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(5402..7303)
                     /gene="ipaA"
                     /locus_tag="OO585_001080"
     CDS             complement(5402..7303)
                     /gene="ipaA"
                     /locus_tag="OO585_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6628737.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDAVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(7312..8310)
                     /gene="ipaD"
                     /locus_tag="OO585_001081"
     CDS             complement(7312..8310)
                     /gene="ipaD"
                     /locus_tag="OO585_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6628738.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(8361..9452)
                     /gene="ipaC"
                     /locus_tag="OO585_001082"
     CDS             complement(8361..9452)
                     /gene="ipaC"
                     /locus_tag="OO585_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6628739.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(9472..11214)
                     /gene="ipaB"
                     /locus_tag="OO585_001083"
     CDS             complement(9472..11214)
                     /gene="ipaB"
                     /locus_tag="OO585_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6628740.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(11220..11687)
                     /gene="ipgC"
                     /locus_tag="OO585_001084"
     CDS             complement(11220..11687)
                     /gene="ipgC"
                     /locus_tag="OO585_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6628741.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11745..12371)
                     /gene="ipgB1"
                     /locus_tag="OO585_001085"
     CDS             complement(11745..12371)
                     /gene="ipgB1"
                     /locus_tag="OO585_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6628742.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(12387..12776)
                     /gene="ipgA"
                     /locus_tag="OO585_001086"
     CDS             complement(12387..12776)
                     /gene="ipgA"
                     /locus_tag="OO585_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6628743.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12789..14273)
                     /gene="icsB"
                     /locus_tag="OO585_001087"
     CDS             complement(12789..14273)
                     /gene="icsB"
                     /locus_tag="OO585_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6628744.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            14587..16203
                     /locus_tag="OO585_001088"
     CDS             14587..16203
                     /locus_tag="OO585_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6628745.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            16213..16575
                     /gene="ipgE"
                     /locus_tag="OO585_001089"
     CDS             16213..16575
                     /gene="ipgE"
                     /locus_tag="OO585_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6628746.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            16575..17033
                     /gene="ipgF"
                     /locus_tag="OO585_001090"
     CDS             16575..17033
                     /gene="ipgF"
                     /locus_tag="OO585_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6628747.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            17042..18157
                     /gene="mxiG"
                     /locus_tag="OO585_001091"
     CDS             17042..18157
                     /gene="mxiG"
                     /locus_tag="OO585_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6628748.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            18165..18416
                     /locus_tag="OO585_001092"
     CDS             18165..18416
                     /locus_tag="OO585_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCR6628749.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            18429..18722
                     /gene="mxiI"
                     /locus_tag="OO585_001093"
     CDS             18429..18722
                     /gene="mxiI"
                     /locus_tag="OO585_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6628750.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18728..19453
                     /gene="mxiJ"
                     /locus_tag="OO585_001094"
     CDS             18728..19453
                     /gene="mxiJ"
                     /locus_tag="OO585_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6628751.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            19450..19977
                     /locus_tag="OO585_001095"
     CDS             19450..19977
                     /locus_tag="OO585_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6628752.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19919..20614
                     /gene="mxiN"
                     /locus_tag="OO585_001096"
     CDS             19919..20614
                     /gene="mxiN"
                     /locus_tag="OO585_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6628753.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20607..21014
                     /gene="mxiM"
                     /locus_tag="OO585_001097"
     CDS             20607..21014
                     /gene="mxiM"
                     /locus_tag="OO585_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6628754.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20998..21426
                     /gene="mxiM"
                     /locus_tag="OO585_001098"
     CDS             20998..21426
                     /gene="mxiM"
                     /locus_tag="OO585_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6628755.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            21556..22188
                     /gene="mxiE"
                     /locus_tag="OO585_001099"
     CDS             21556..22188
                     /gene="mxiE"
                     /locus_tag="OO585_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6628756.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            22175..23875
                     /gene="mxiD"
                     /locus_tag="OO585_001100"
     CDS             22175..23875
                     /gene="mxiD"
                     /locus_tag="OO585_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6628757.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23894..24961
                     /gene="mxiC"
                     /locus_tag="OO585_001101"
     CDS             23894..24961
                     /gene="mxiC"
                     /locus_tag="OO585_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6628758.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24974..27034
                     /gene="mxiA"
                     /locus_tag="OO585_001102"
                     /gene_synonym="virH"
     CDS             24974..27034
                     /gene="mxiA"
                     /locus_tag="OO585_001102"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6628759.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            27047..27448
                     /gene="spa15"
                     /locus_tag="OO585_001103"
                     /gene_synonym="spaK"
     CDS             27047..27448
                     /gene="spa15"
                     /locus_tag="OO585_001103"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6628760.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            27452..28744
                     /gene="spaL"
                     /locus_tag="OO585_001104"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             27452..28744
                     /gene="spaL"
                     /locus_tag="OO585_001104"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6628761.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            28837..29175
                     /gene="spa13"
                     /locus_tag="OO585_001105"
                     /gene_synonym="spaM"
     CDS             28837..29175
                     /gene="spa13"
                     /locus_tag="OO585_001105"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6628762.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            29162..30040
                     /gene="spa32"
                     /locus_tag="OO585_001106"
     CDS             29162..30040
                     /gene="spa32"
                     /locus_tag="OO585_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6628763.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            30034..30915
                     /gene="spa33"
                     /locus_tag="OO585_001107"
                     /gene_synonym="spaO"
     CDS             30034..30915
                     /gene="spa33"
                     /locus_tag="OO585_001107"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6628764.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30920..31570
                     /gene="spa24"
                     /locus_tag="OO585_001108"
                     /gene_synonym="spaP"
     CDS             30920..31570
                     /gene="spa24"
                     /locus_tag="OO585_001108"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6628765.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            31585..31845
                     /gene="spaQ"
                     /locus_tag="OO585_001109"
     CDS             31585..31845
                     /gene="spaQ"
                     /locus_tag="OO585_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6628766.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31845..32615
                     /gene="spa29"
                     /locus_tag="OO585_001110"
                     /gene_synonym="spaR"
     CDS             31845..32615
                     /gene="spa29"
                     /locus_tag="OO585_001110"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6628767.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32622..33650
                     /gene="spa40"
                     /locus_tag="OO585_001111"
                     /gene_synonym="spaS"
     CDS             32622..33650
                     /gene="spa40"
                     /locus_tag="OO585_001111"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6628768.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33663..33905
                     /locus_tag="OO585_001112"
     CDS             33663..33905
                     /locus_tag="OO585_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628769.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10720 a         5140 c         6967 g        11742 t
ORIGIN      
        1 caccagattt tcaccaggat gcacgtcata ctcttttttc tccggaggca gtgccagcat
       61 ggactgctgc tcttcgagcc agcgatcaca gggacgggcc tggattgttt catgctttcg
      121 ttggttagcg acatcgtgca gccagcgcag accgtggtgg ttggctgttt caacatcgac
      181 agtgctcccc atcgggcgca ggcgagtcat tagtgggatg taaaaactgt tccatccgtt
      241 ccaccttacc tttagtctgt gccctgaagg ggcgacacag tcggggagag aagcccatct
      301 ccttgccgaa ctgccacagc gaaggatgga accggtgctg accggtctga tatgcgtcac
      361 gttgcagaac cacagttttc atattgtcat acaacacttc gcgcggcaca ccaccaaaga
      421 agcggaacgc attacgatgg caggtctcca gcgtgtcata acgcatattg tcagtgaatt
      481 cgatgtacaa cattcggctg tatccgggaa cagcaacgaa cacgtgaagc ggtgagcgac
      541 cattacgcat agtgccccag tcaacctgca tctgtcgtcc gggttcagtt tcgaaccgaa
      601 cggcaggctc ctgctcctga ggaaccgaga gagaacgaat gaatgccctg agaatggtca
      661 ttccgccacg atatccctgg tctctgatct cgcgagcgat taccgttgcc gggattttgt
      721 aaggatgagc atcggcgatg cgttgacgaa tataatcccg gtattcatcc aggagtgaag
      781 caacagcagg tcgcggcgta tattttggcg gctcagattt tgcctgcaaa taacgtttaa
      841 cggtattgcg ggagagcccc agttctctgg caatcgcccg gctactcatt ccctgcttgt
      901 gcaggatttt aatttccata actgtctcaa aagtgaccat aagctctcct gaatcaggag
      961 agcagattac cccctggatc tgatttcagg cgttgggtgt ggatcactat tgcaccgttc
     1021 gtgacacttg ttgactgcct gtgcggcatc tggtatcaag gtggcgtgaa gccggttcag
     1081 gttctcatgc ggaacgttgg aacctatatc cccccgatac caaaggcgaa atgtaagtga
     1141 caaaatgcaa tgaacacgtt ctccgttaac tgaggagcat ggtgataaat aaaggtaaat
     1201 gcagaaggtg tagagtctcg cagatttctg acatcataga gcctaacatt agatgcataa
     1261 cgctacagat tgtagaatct ctacaggtga aatatacact ggttggcgtt tatgtcgcaa
     1321 aacatgtcat tcaggttcat ctcaccaata aggatatgag tgaagtggag gataagtgag
     1381 tctgctgtca gagttttctg gtgtatgtca gtaactgcga accatgtctg cttgatatgg
     1441 gagcttatgg aggctctctg tacaaggtta gggagctaac aaagcaggga taaaaagtcc
     1501 gcctgatgca ggcacggttc gttaaatctt ttgccattgg taacaaaatg gtgcgatgaa
     1561 tgttcaggct atctttatct tgatggtggt tcagctgttt ggtaaagaaa tcgctgtaac
     1621 aatagaagaa ctgcaggcag tactggtccc acatcgtttg ctcagtcaac tggtgaagtt
     1681 ccgtagcgcg taaattaatg ctttgcatga aacgttactg gagtggatga aagaatacct
     1741 gcatatatca ttactgcgtt ggattaccag tacaacagat tgaatgaact ggacatgttg
     1801 atagacggca ttgaaaagca gcttataagc atagtgcggc agagttaatt ggagagcact
     1861 tcgccttctg agtattaagt aatcatggtg gtacctgcgg atactatcta ttcactgtgc
     1921 tcggtcggca gccttactga ccaaggagtt gggggagttg ataacaaagt tgaaaaaaca
     1981 gctctcaacc aaggtggtta ttgtaacaat agcaaataag ctggtgtgac ctgtatcaca
     2041 ataaccgatt atggacgtaa atacgacaaa aatatgtcag tatcaggcgg tattaattgc
     2101 tgatgctgat ggacattaac tcttaataaa agggtggatg ctgaaaggtt gttatggtga
     2161 cggacgtgat gacgacggaa aagagtatga ggttttggat ttaaagttcg ttcggggaat
     2221 aaggacccgg tcagctaatt ctcatagtag ttcgcattgc tggatgcaat actgtgctgc
     2281 aattactcgt cgatgtcgga acaacggaag ccttgcaaac ggggatgtat tcatacaggt
     2341 gttatgttat atggggtttt attgcagggg gctataccaa gagaatatat gatatctcat
     2401 cagactgatg tgagggtaaa tgagaataga gtaaacgaac aaggttgttt tttggcgagg
     2461 aagcagatgt atgacaattc ttgtggagct gccagtttac tctgtgctgc aaaggaatta
     2521 ggggttgata aaataccaca atacaaaggg tctatgtccg aaatgacaag gaagagttct
     2581 cttgatttgg ataatcgttg tgagcgggat ttatacttaa taactagtgg taattataat
     2641 ccgagaatac acaaagataa tatcgcggat gccggttatt ctatgcctga taagatagtc
     2701 atggctacga ggcttcttgg attgaatgct tatgtcgttg aagaaagtaa cattttttcg
     2761 caagtaatta gttttattta tcccgatgca agggacttat taattggcat gggatgtaat
     2821 atagtacatc aacgagatgt gttatcttct aaccaaagag ttttggaagc tgttgctgta
     2881 tcctttattg gagttcctgt cggcctccac tgggtgttgt gtagacctga tggaagctat
     2941 atggatccag ctgtaggaga aaattattct tgtttttcaa ctatggaatt aggtgcaaga
     3001 agaagtaatt ctaactttat aggatatact aaaataggta tttccatagt tataactaat
     3061 gaggctttgt aaattgtcgt tttttatctg tgaaggagct tgaatatttt ggggcggggg
     3121 cataacaaat gatattactg agtacaaatt tagctatatt gggcggatta gttgtgtggg
     3181 aagacgaaat gtttctcttg aaataagtga actccctcaa ggaatgattg tgtgcttaaa
     3241 tatttaagtg ctggtaagtt tgagaaggct gacatatttt gcagagcaaa cagtgattga
     3301 cttggatgat atttttattt tagtcactcg ttgtaattgg agtatgcttt attttatttg
     3361 aaaatagatg tattttatac ccgcaattta aactgtaagc cctatgaggg gtatggatat
     3421 tataagttac tctttgacta agcataactt ttggggttag aaaaataaat ctcttgttaa
     3481 taattaatat gattttaaaa tcaaacttgt ttataattat aaaatcatgt aagtcggtca
     3541 ttttatggga agattaggag aataaaacag taattatatt tgagaaatct gctaatatat
     3601 tcttaaagct tttttgatac tgtgaacgaa gtctgttaat ggtggatttt ttgtttatca
     3661 ccatgtaaaa aattgagtag taaaagcgtt gactgctaat cctcgctgtt tttattgaaa
     3721 cgattaaaaa tacgctgaat attttcggcc agtcactcat cttcacataa aattttgacc
     3781 atcatgccgc atcccttagt tgcacaattc attttcaatg atatgaatat tactaatttc
     3841 accagataat gttctcgtgg tcagcagggt atgagttttc accgtaattt taaggaaaca
     3901 cagtgcgtgg ggattgaata acagatagcc gacggaatta ccccaccggc tgaaagtcaa
     3961 gttcaacgaa gatttttatt atctgaattg ggcagtttac atcagtgttc gatgtttatg
     4021 aagacgatag atggcgagaa attatatctc gaatagcttc atctatttct ctctgaactg
     4081 ttttgtttat tttagataat gtaaatatta gagttctgtt tgttttattc tcttttcttt
     4141 ttatgtaagt ccgtttgtct ttagaaataa ataacgagtc aacttttagc gaagggtttt
     4201 gctttctgtt tttgcttttc tttatgatgt ttaaaatttc ttttttatat atgtccgggg
     4261 gcaaatttgt atcaaggtct tttatttctt cctttaatat tggtagtgta gaactaagag
     4321 attcattagc cttttcaagt cctttataat aattgaataa tatcttgtag tcattaaagt
     4381 taagttctga cgcgattgga aatagagata ttatttcctg tggaacgctt gctgcctgaa
     4441 aggcacgagt gactttcgcg cgagacagat tctctttttt ggctatgtct ttataggaca
     4501 tccctgatac tttcagaaaa ttaagaccaa taccaagttc tcggatgcta tgctcttttg
     4561 ctgtttgtat atcgtttgct agttttctgg catcaagagt agatatatat tcttttgaat
     4621 atagaacttc aagatctgct cctgcatata ttgcagatgc tcttctacga gtgccatcca
     4681 gaatttcaat tctaccatca atctcccttc ctattacagg gaagaattgt tgtagcttta
     4741 tagtttttat gatatcggct aatgattctt ctgttaggga ttcttgatcg cgtacattcg
     4801 ttttttggtt gacgaaggtt aaatcttgga tccttttatg aggaatcttg gctttgataa
     4861 aggaaacttt attaccagaa tggagtgtaa actctttctt ttggccttcc tttatactta
     4921 acaagtcgtt gcacaaatcc accatatcac accctgttta ttcatattga tttatattga
     4981 caaaagttaa atgcagtgtg agcaagattg tatgtaactg ttgatgatag tcaacggtag
     5041 tcttaaaaat tactatttta atagctggat tgatgcaatt tatctttatt ggataaaaga
     5101 ataatattct tgtggagctc tgatgtgaga gctccataga tgaaaaaatg cttatcaatt
     5161 actatattga ttttgtacta tttttgacta cagaaataag atctccaata gtaataatgt
     5221 gttctaatgc agattcatct atacgtagat tgaactcaaa actaatcata tccagcattt
     5281 caatcagaga aagagaatcg gcagaaagat cttcaataaa tttagtttct tcagatagtt
     5341 tgctgtacgc tataccatag caaaaggcca ctattgataa tattttttct tttatcatat
     5401 tttaatcctt attgatattc tttaatactt ttgataggga agtggttaca tcttttgctg
     5461 ctttatatat attattgttt tcagctgtaa tatcattgtt attttttaaa tcagatatat
     5521 catctgtaag taattctgca gaggtatcgt caatttttga caataccttg ctgagggcgc
     5581 tagatacttc ttttgccttt tcatagatcg catgattttt atcaattgta tctgcgttgg
     5641 tttctgttac gtagtttatt gtatttgcat ctgtaacttt taatttgttg tttattctgt
     5701 gttctgttgt ggattttgta atgtcctttg atttttcctg taactctcgt tcttgtgttc
     5761 ctgatttagt tccaatgaga gatataagat tacttaggga gtttgttact ttttttgaag
     5821 cttcaaatat cgtttcccgt gttaaatatg tgtctccatt tcttgataaa aaatcagtct
     5881 gaagtacttt tgaatcagtg tttgagttga atttattgat ttgcgaagct actctatgag
     5941 aaagagaagc gctactttta gttgtatctg atgagttatc attactgctt gtcactcctt
     6001 ccgggtggag agggggggtg gttgaagagt tctgtatgtt cagaggcctg tgtccccgag
     6061 aaagagagtt atttgtagat ctttcttgac tactcgtagt taacaggtct gaacctattc
     6121 catccgaatt atctggagta tggttgaaaa ccttttctaa agctgaatta tttgcaaatg
     6181 tctggacagc atctgatata gagtgtggta gaacggaagt tactttttga actagtgctc
     6241 tttgtggatt tagtaatgag tttccgttac gggataatag atcgtttgtc tgagaagtag
     6301 gagactgtgc atcattttct gggttctcgt gatagctatt gtcatatact ctgttgtcaa
     6361 aaactctatt atcgtaagtt ctattatcat tatttatatg atagtgaatt acaggttggg
     6421 atgttttctc attggctccg ggggttggtc cgccatctgg catatcttcg ggaggtgtag
     6481 ggaggccatc ttggatagga tgcgaatctt ttatatcttc taaaatatca ttaaggactt
     6541 ttctcaacag ctcaggaaac acggctactg tatttaagtc tcttgacagt ttacctatac
     6601 caaatccttt actatcaata gtatttttac gttgattgcg caaactatca aaggcaagtt
     6661 ttgcctgtgc gtcaactaaa tcatctagtt tgagagatga aacagtttca ttacttaact
     6721 ttaattcagc aagaatatta ttcttgataa atgatgatac ttcagtattg aggagttttt
     6781 gtcccaatga taaagcttca ccagatgtgt gttgttgtgc taatagtgca agtctgtgct
     6841 caatgttttg tcgtaattga tggagtttct tattctttgc cgaccatggt ccaaaggcag
     6901 aatcttggga taatttatct tttataatta gttccgaata ctgtgcgaca attttttgaa
     6961 gtcccttttc gttttcaaaa ttaagatcat tttgatgtac taaggagtac aaaacatttg
     7021 aaaattcgag aatctcgctt ttggcatacg cattcattga gttggaataa atgcttctta
     7081 acacatcttc tttccctgag gaagaaaata aaaaatcaag acatttctga ttaaaagacg
     7141 ttgcaaagtt ttctttttca gacactgatg ctggtgtttt tagagaagtt tgcgaactat
     7201 ggatatcgga tattttgctt tttaactcgc tgtattctgt tgatgatggt gaagttgcct
     7261 tatataagaa tgttggcgct tgagtattat ttacattatg catggcgcac ctcagaaatg
     7321 gagaaaaagt ttatctgtat ctgtacatga gcttattgta ctactcaaaa cctttactaa
     7381 attatcaaaa atactattgg cattactgta tttttgaact aaagtttgaa gattattttt
     7441 cattgtttca tcttcggcag agaatccggc attccatgcc tgatattttg cattatctag
     7501 cacaacctcg ccatttccac ctagattatc taagcttttt aacatattgt ctattggggt
     7561 catgtttata ttgacaacat atcccccgtt tttttcagat accttgccga ttgttccacc
     7621 taattctgta agccatttat ttgcttgttc cttactaaca gtattatttg ctggatatag
     7681 cggtttatct ttatattttt ccttgagctt tgtcaattcg tctttaagcg atttgacttg
     7741 taatttcacg gagtttccgt cgttacctcc gggagagatc cagccggcaa gactggaaag
     7801 aacagcgcta aaatcttgat acatttgagt atatgaacta acggcatgtt catatacttt
     7861 cagatactgt tcattaatat tattgattga ctttgcaatt ttatcccaca gttctctgtg
     7921 agatatcatt tcatatccat caagctcggc ttctttcggg gctgaatgta ataattctct
     7981 tgcgtcttta ttaattggat attctttctt ggaaagaata tccaatagtt gagcggattt
     8041 gtttacatcc atgctaatct gagatgaatg taatgctatt tcttctagcg atgttttagt
     8101 caacgttttt tgtgagagat ctttctttaa tgcctgattt gttgttctga tattatgaag
     8161 ggtgtcgttg agcatagtaa gggaacttac aggatgagaa ctggtcgttg tttttatatc
     8221 agaattaact gtttcggttg atgaaccgtt ggtattgttt gggctgaatg atgaggtgga
     8281 aatactatta gtcagagttg ttatattcat ggttatttcc ttataggaag agccatatat
     8341 tattatggct cttggatata ttaagctcga atgttaccag caatctgact ggctgtagaa
     8401 ttccttgatt ggttgatgtt gtcaattata ttcaataatt tttgtattaa ttgatttgtc
     8461 gcttgggatg cttctttaga tacttgggat gcttcctctg cttgtttact gctggcctga
     8521 ctgattagtt gttcttcttc ttcaagagca gatgtataac gccctcctga tgtggatata
     8581 ttaccagcaa cggctgatgt ctccattgtt gcacggccaa tgttttgttt ttgctgagca
     8641 gaaagggtat tgagctcgta agctcttctc tgggtgtcaa ttttatcctg caaagaaata
     8701 tcgggagaac taagagaagt tttatgttcc gttgacaggg atatattatc tggtgccagt
     8761 ttatttttac ctaaaatttt tgtgctagag ttagtttgtg gtgaggtgat atttgtatct
     8821 atttgtttat ttaaccctaa tttagaacct gcaagctctt tttcaagaga ttgagcagtg
     8881 gcaaggttct ttcttaaggc tcctttttgt tcgctaatcc ctgaatgcgt ttttttggca
     8941 ccgatacccg ttatacctac ttgtgtgacc gctccagtaa tgcttgatga tagggctgct
     9001 aggccttgcc gaacaatgtt ctctgcagct gattttgtag catcgaacgc aatcaatgac
     9061 aattgagagc ccaatctagt ttctgcagtg cggagagcag aaagtagaac ggctacacta
     9121 ataattaaag aaacagcatt agaagaaaga ctggaaatat ccagggtgtt ctctggggta
     9181 attgtgtgac tcatttctga aagatctttc atctttttgt cagtaaggcg agcaatgatt
     9241 tctgaatcat gctgaacctg ctctgataac tttaaaagtt gatcatcatt taatgtagtt
     9301 gttaatacgg gatttttacc gacattaagt ggaatatgtg ctgctaactg ctgataattt
     9361 tgtgattttt gtgtttcgga agaactttga gtttgttttg tagatatatc tgtatataaa
     9421 atcggggctg attttgtgtt ttgaatttcc ataacattct ccttatttgt atcaagcagt
     9481 agtttgttgc aaaattgctt ttgcaacatc agttctatta gcctgagagt tggacattga
     9541 ggctaacagc tctgcaatta cttcctgcaa ttggccgaat ttttctattg cgtcaccgat
     9601 gtattttgac agttgttcaa cttgatattt cgataatgtc aggtctgcta gatttttcga
     9661 cgcgttgttt tggaaaacag cagaagcgac atttcctccc gcttgtgtgg cagagttaac
     9721 actttctcct aataaaactg cttggtttaa atgggtggaa ataatttgtt tggatattac
     9781 ttcatcaccc gctgcaccaa gaaatttatt taatctggca acagcattag tgattaaatc
     9841 gtccagttga gaagaaaaat tcttgagaaa ctttggtata aggtctgtga gggttttacc
     9901 tattattttg ccaatattct ctgcaagttt tgctgctgcc tgtttaccaa cagtggctac
     9961 gagaacgact gctgcaacca agacaagagc gcctgcgatt gcccccagaa tagagccaat
    10021 cattttggct tttttcgagt cgacgcccaa gccttcgagc atttttgtaa atgcatctga
    10081 aaggagtttg attaagggtt caatgactgc tttcatgatc ggattcaggg cttgttccat
    10141 gaaggaattg ccggtcgctg cttgtactat agcatccgta accataagag ctaaaccaac
    10201 agctgccagt gctagagatg ctcctccaga aaaagctgct gcaacaacac taacgatagt
    10261 taaaagtgcc ccaagtattt tcccaacaca acccattact ctgttgagtt cttctgcttt
    10321 acgtacttca gcagcatact catcagattt tctctccatt tcagtttttc ttgattcttg
    10381 gagagactgg aatagagcca gatcattttt taaagattct tcattatttt ttccaactag
    10441 ttgaataaag gttgccatca attgagtaac actggcaagt ccggttaatg atttctgctg
    10501 ggttgatagc tgttcagcag atgctgtgtt tgaaaatgca gaaaaagagt ctatttcttt
    10561 ttcgagttgc attgatttat ctgtaagttt gctatgaatt gacagagttt tctgctcaat
    10621 caatgtcctg tctttaactg ctgcgtcttt tttgatagtg agttgtattt cttcccgtct
    10681 taatttttta ttttctggtg aatctggggc gagttcggat aatcttgttt gaatttggtt
    10741 aattttattt tctaggtctt ttatttttga atctgcgttt tttagtttat taatttgttt
    10801 ttcatagtct ctggttagtc cttcagtttc agatagaaga gtattaattt tatcggagaa
    10861 ttctaggttt ttttgctgtc ttgcctgttg ctgggacttc caggcagtaa ttttatttgt
    10921 taatgcagtt aaagattttt caccaagtat ttgaataagg tttccaatta aaagcgttag
    10981 ttgggaactt gcatttaatg actttggtgc tttaagttca gggattaata tattcgaagt
    11041 tgagtgtgca ttagttgtat taatattttt gttagcagca agatcagcaa ttgtaagaga
    11101 aaataatttg ttagctgcat catttgcagc ctggatagta ttgtctccaa gctcagtgga
    11161 agccaatatt ttggcaagag gcaaaccagt ggttgtggtg cttacattat gcataataat
    11221 tactccttga tatcctgaat tgcgtctaag tatgattgtg cttttatttt taatttttca
    11281 tcattgctgt gttgaattac gagttcgaag cactctttgg ctttcaaggg ggctttcaac
    11341 cgaagctggc attgtccagt atggaatacc ggtgtatagt cattttttcc taatgcaaaa
    11401 gcgacagcat aaaggtctgc tgcttgttgg aactgttctt ttatctgata aatagctgcg
    11461 agtcccataa tgtagtctac attgtaaaag tcgtatatac ataaaaatct gaagaaaact
    11521 tcagcttcct ctattcttcc tttgttgtaa aagtcataag catatgaata aatgtcatcc
    11581 atcatatcat caggaattgc attaatatct ttcagtgtag cgccagagtt aattgcatca
    11641 attactgcag tagagatgct ttcattttca gtgatattta aagacataag gtctcctttt
    11701 tgtctgcatt actttaataa gtataagatt taatataaac agatttaatt tgtattgctt
    11761 tgacggtata cagcctttag aacctctgcg cttatttcta tttgtaaaac ttttgttgat
    11821 tcattaaata ttttatcgta gacggaactg gatactagat ttgtaagatc cttcatttgt
    11881 gtgttaaaca aatccatgtc actgctataa cgttttaaaa tctttgctct gaggccagat
    11941 gaggcaatct cgtttagtgc tgtatgactg atagatgttt gtgctgattt ttcatcaagt
    12001 tttatatccg ggaatttttt ttcaatagct gcaaatatgt tttttttgtt atctgaggtc
    12061 aaaacaactc cattgtgtat ggccatttca tctatacttc tgtttatcat tgaagagaca
    12121 taagaggttc gctcttggct catccaacaa aatagttgat tccccgaatt actatctttt
    12181 attttgtcta tgacgctgtc cctgatttta tttatattca ccgggcatcc aaaattcttc
    12241 tgaggaaagc gttgttttaa gttaaaaaca gataatattt tctttacaag cttattgtga
    12301 cttaacgaat caaatgatgg gcgaggaatc gcgaattcaa cctgtggaag tattttgttt
    12361 agaatttgca tgataccccc tatatgttag ttcacttctg aagtgatgtt tgcaatggtt
    12421 agtttaatac aaaataaaaa ttcgtacaaa gattcaatga gttcatcagg tgaaagatat
    12481 tttttgtagt gtttcttggt caaaacacat tgatgagtat cagggagaag acagatagaa
    12541 gaaccataat ctctactcaa agagtagtta aggatgagta tgtgttctgg attaataata
    12601 ttttctgtaa aaagttcatg ttcattaaga agtccggtca aaaagatgta ttcatcatta
    12661 tctgttatac aaacaggtat ttgttcttcg agcaatataa aggcttgatt tttatcgtta
    12721 aaatcaagct ttgcacctgt tatttcataa agtttatcat atagtttgcg acacatttct
    12781 atggccttct atatattaga atgagagtta ttcaataaaa gcttattgac ctgtaataag
    12841 tcgtgaatgt aaagtttggt tccaagatct ggcgatttaa gagaattgta ataatcaata
    12901 gtttttattg acttttcgat aattacttct tttttatctg ttgctccggt ttttaattcg
    12961 tttaatacac gagacatatt gctgggtgct gattttacta agtatgagtg gcaagcgttg
    13021 atgaccgcat caaagtcttc tgaagataca ttttcagtag gatgtttgta aagtgaaatt
    13081 gataaaggct tgaatgtttt tggggtgtgg ataaggttaa ttttattgaa acttttaaga
    13141 tagctacgtc gtaattgagt caatctggaa agatcaaaat cagattcaat atacttcatt
    13201 atctcgttat aagactgatt gagactttca atgcgttgcc taactttatt tgcatacgcg
    13261 tatacattgt taggtgtagc aaccaattta acgtcaggga gtggaaagta tatttcagaa
    13321 tttcctgctt taagaacatt aagtgccata ccagcacagt tttcagattt actgattaat
    13381 ttataatttg ccaattgtct gaaagcatct gctttctttt ggcatataaa ttttgacatg
    13441 tttgtttcat caaggccaaa aaggttatag ctgattttac catcctttgt tttattatca
    13501 cctgataatg gaatgtatac tttatcagca ctgacccccc aataaccata ggggtctttt
    13561 ttttgattag ctctgggaaa aaatattttt ttgttgttat tttgatcttg ttttgaattt
    13621 aataagtttc taacctcttg gcctgcatca agtctttcgg ctgtttggtc tgaaatcata
    13681 tttagtttgt cttgtttata ggtatcgaca ctgtaacctg agcgtttttc aaaaaggtag
    13741 tttatttctg tatcttttgt aacattcccg ccgggatacc agctgagata ggtatgagtt
    13801 ggttcctgta gtaaattgtt ttttatactg atactggtat gccctaaatg ggttatgcct
    13861 ctatttcccc agcaacgaaa cactaaatgt ctttcaagat ttttggctgt accgatgcca
    13921 tgaaaactct ctgcggtatt caacattaat ttgatgtctt tacctgtaaa agaatgttga
    13981 attgaatttt tgactgcttc ataggcggca aacgcacttt ctgcagattt ttcgctagct
    14041 aatgtcttga ggaaaagcaa taaggatttt tgattcgcta gtcgataatt ttttagttgt
    14101 tcatttaata tctgtgaatt tatctttgca ttaatagtgc ttaatgttct gaaaaagaga
    14161 tcctttaggt tgaatttctg tgctaccaat ataggtccat gctctgtatc ttcaacgcgt
    14221 ataggccctt ttgtattgct tgcgtcaatg aaattgctaa ttttgagaat catactttat
    14281 taactctcca ttacgtggtg attgtgggga tgtaaaatgt atttgatata taatatattc
    14341 aattaataaa tttagaaact tgagcctgtt aacataatca aattttcttt tgctgtacat
    14401 aatatgtacc tcgtgagcat atgtagtgct ggtttcatca tgaaatccca caagttaaag
    14461 tgtctgatat atcaggctcg gagtgttata gaaaaagaga gaaccctgtt gaataagtat
    14521 cttttttgtt gtaaaacgag gtacaaaact gcaatcaatg ccccatttat gactgaggat
    14581 aattaaatgc acataactaa tttgggattg catcaggttt catttcaaag cggagattcc
    14641 tataaaggcg cagaagaaac cggaaaccaa aaaggtgtaa gcgtgatttc atatcaaaga
    14701 gttaaaaatg gagagagaaa taagggggtc gaagcgctta atcgattata tttacagaac
    14761 caaacaagct taacagggaa aggccttttg tttgcgcgtg acagagctgc agttttttac
    14821 gaggcaataa agttggcagg tggtgatacg tcaaaaatta aagccatgat ggaacaatta
    14881 gatacctata aacttggtga agttgataaa agaaatatta atgagcttaa taaagtaata
    14941 agtgaagaga taagagcaca gctaggcatt aaaaataaaa aggaattgca atcggaaata
    15001 aaacaaatat ttactaatta tttaaataat aagaattggg aaccgatcaa caaaaatatt
    15061 aattataatg ggaaaaatta tggttttcaa ttaacacccg cctctcatat gaagataggg
    15121 aataaaaata tatttgtcaa agagtataat ggaaaaggaa tttgttgtgc ttctacgaga
    15181 gagagtaagc atattgcgaa tatgtggctt tcaaaagtgg tggatgacga ggggaaggaa
    15241 attttttcgg gtatcagaca tggagttata tcagcctatg gattgaaaaa aaactcatct
    15301 gaaagagctg ttgcagctcg taataaagct gaggagttag caagcgcagc attatatagc
    15361 agacaagaat tattatcaca ggctttgtct ggtaaaacag tagatttaaa gattgtttcg
    15421 acttctcttc tgacgccgac cagtttaact gggggagagg agagtatgtt aaaagatcag
    15481 gtaaaggcat taaaagagtt aaactctaaa aggggggaac caacgaaatt attaattcgg
    15541 aatagtgatg gtcttctgca agaagtaaag gttaatctaa aagtggtgac atttaacttt
    15601 ggtgtaaatg aattagcgct aaaaatgggc ttaggctgga ggaatgttga caagcttaat
    15661 ggtgaatcaa tatgttcttt gttgggggat aatttcctca aaaatggtgt gattggaggt
    15721 tgggctgcag aagctataga aaaaaatcca ccatgtaaga atgatgtgat atatctggct
    15781 aaccagataa aagagattct aaacaagaag ttacaaaaaa atgataatgg agagccgtat
    15841 aaattgtcac aaagaatggc tctattggct tatactatag gtgctgtacc ttgctggaat
    15901 tgtaagagtg ggaaggacag aacaggtatg caggatgctg aaattaaaag agaaataata
    15961 agaaaacatg aaacagggca gttttctcaa ttaaatagta aattatcctc agaagagaaa
    16021 agattatttt ctactattct aatgaatagt ggtaatatgg aaatccaaga gatgaatact
    16081 ggtgtgcctg gaaataaagt tatgaaaaaa ttaccgctat cttcgttaga gctgtcttat
    16141 gctgaaagaa taggggaccc caaaatatgg aatatggtga aagggtattc gtcatttgta
    16201 taagaggaat atatggaaga tttagcagat gttatttgcc gagccttggg tatcccttca
    16261 attgataccg atgatcaagc aatcatgctt gatgatgatg tgttaatata tattgaaaaa
    16321 gaaggagatt caataaattt attatgtcct ttttgtgcat tgcctgaaaa tataaatgat
    16381 cttatatatg cgttgagcct aaattactca gaaaagatat gtttagctac tgatgatgaa
    16441 ggtggaagtc taatcgcgcg attagatctt accgggataa atgaattcga ggatatatat
    16501 gttaatacag aatattatat ttcgcgtgtg cgttggctga aggatgaatt tgcgcgaaga
    16561 atgaaggggt attaatgtcc cgtttcgtat ttatttttct gtgttttatc ccatatttag
    16621 ggagagctga ttgttgggat aaggctggtg aaaggtacaa cattccatcg agtttgttaa
    16681 aagcgattgc ggaaaaagaa tccggattta ataaatttgc agtcaatgtt aataacaatg
    16741 gaagtaaaga ttatggtata atgcaaataa atgactttca ttctaaaaga cttagagaaa
    16801 tgggatatac tgaggaaatg ctaattagtc atccatgtct ttctgtacat tatgcagcaa
    16861 agttattaaa tgagtttatg ctgatgtatg gaagagggtg ggaggctgtt ggtgcctata
    16921 atgcaggaac atctccgaaa aagaaaaaag aaagattaaa gtacgcagag gatatctata
    16981 aacgctattt gcggatagca gcagagagca agcagaataa tcgaaggata taagaggatt
    17041 tatgtctgag gcaaagaact caaatcttgc accattcagg ttattagtga aactcacaaa
    17101 cggtgtcggg gatgagtttc cgttatatta tgggaataac ttaattgttc tcggacggac
    17161 gatagaaaca cttgagtttg gaaacgataa tttcccagaa aatattattc cagtaacaga
    17221 tagtaaatcg gatggtataa tttatttggc cataagtaaa gataatatct gtcaatttag
    17281 tgatgaaaaa ggtgaacaaa ttgatatcaa ttcacaattt aactcatttg aatatgacgg
    17341 agtttctttt caacttaaaa atatgatgga ggataaatcg agagttcata tactaaacgg
    17401 aatgtataat aaaccctcgg tgtttttttt ctttgctgtt attgttgtgt taattataat
    17461 tttttccctc tctttaaaga aagatgaagt aaaagaaatt gctgaaatta tagatgataa
    17521 gagatatgga atagtcaaca caggacagtg caattatatt cttgccgaaa cacaaaatga
    17581 tgcggtatgg gcatctgtag ccttaaataa aagcggattt accaaatgca gatatattct
    17641 ggtgtccaac aaagaaataa atagaattca acaatatatc aatcaacgat tcccatatat
    17701 caatttgtat gtgttgaatt tagtgagtga taaagcggaa ttactggtat ttttaagtaa
    17761 agaaagaaat agttcaaaag ataccgaact tgagaaattg aaaaatgctc tgatagttga
    17821 atttccgtat atcaaaaata ttaaattcaa ctatcttagc gatcataatg caagggagga
    17881 tgctaaaggt attttcacaa aggtaaatgt ccagtataaa gagatttgtg aaactaataa
    17941 agttacatat tctgtcagag aagaactgac agatgaaaag cttgagctca ttaatagact
    18001 gatttctgat cataaaaatg tatatggaga tcagtatatt gagttttctg tacttttgat
    18061 agatgatgat tttaaaggta aatcatatct taacagcaaa gacagttatg taatgttgaa
    18121 tgataaacac tggttttttt tagataaaaa taagtgagga taaaatgagt gttacagtac
    18181 cggataaaga ttggactctg agttcattat ctgaaacttt tgatgatgga actcaaacat
    18241 tacaagggca actaacatcg gcactaaatg cattagctga aaatccttcg aatccacagt
    18301 tgctggctga ataccaaagt aaattatctg aatatacatt atataggaac gcgcaatcta
    18361 atactgtgaa agtgattaag gatgttgatg ctgcaattat tcaaaacttc agataatagg
    18421 gagcattcat gaattacatt tatccagtca atcaggttga tattatcaaa gccagtgatt
    18481 ttcaatctca agagatatca agcctggaag acgtcgtgtc ggctaaatat agtgatatta
    18541 agatggatac agatattcaa gtatcacaaa taatggagat ggtaagccat ccagaatcat
    18601 taaacccaga atctttggcc aagttacaga cgacgctctc aaattattca ataggagtat
    18661 cattagctgg cacgttagca agaaaaacag tttcggctgt tgaaacttta ttaaagtctt
    18721 aatttatatg attaggtata aatgttttat tttattcttg ttgctgatgt tgattggatg
    18781 tgagcaacgt gaagagttaa tttctaattt atctcaaaga caggcaaatg aaataatatc
    18841 tgtgctagaa cgccataata ttactgctag aaaagttgat ggaggtaaac aggggatctc
    18901 ggtacaagtc gaaaagggga catttgcatc ggcagttgat ttgatgcgca tgtacgattt
    18961 gccaaatccg gagagagttg atatctcaca aatgtttcct acagattcat tagtgtcttc
    19021 tccaagagct gaaaaggccc gtttatatag tgctattgag caacggctgg aacagtcttt
    19081 agtttctatt ggtggtgtta tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa
    19141 aaatatatct tcaaaaccga tgcatatatc agtaatcgct atatatgact caccgaaaga
    19201 gtctgaacta ttagttagta atattaagcg atttttgaaa aacacctttt ctgatgttaa
    19261 gtatgaaaat atatctgtca tattaactcc gaaagaagaa tatgtttata caaatgtaca
    19321 acctgttaag gaaattaaat cggaattttt aacaaatgaa gtaatatatg tatttctcgg
    19381 gatggctgta ctagttgtca ttcttttggt atgggcattc aaaacagggt ggttcaagag
    19441 aaacaaaata tgataagaat ggatggaatt tataaaaaat atctttcaat aatttttgat
    19501 ccagcgttct atataaatag aaatcggtta aatttgcctt ctgaactgtt agaaaatggc
    19561 gtaatcagaa gtgagattaa taatctcata attaacaaat atgatttaaa ttgcgatatt
    19621 gaacctttaa gcggggtaac cgctatgttt gttgccaact ggaatttact tccagctgtt
    19681 gcttatttta tcggtagtca agagtctcgg ttaattaatc atagtgagat ggttattagt
    19741 tattatgggg gcaaaatcag taagcaaggg gaggccgcta ttcgaagtgg tttttggcat
    19801 cttattgagt ggaaggaaaa tatatctgtt ggtatatatg aaagaataaa cttattattt
    19861 aatccaattg ctttggaggg aaattatacg cctgtagaaa gaaatttgtc ccgattaaat
    19921 gaaggtatgc aatatgcaaa aaggcacttt accggtatcc agacatcatg cctatgatgg
    19981 tgttgttata aagagaattg aaaaggaatt atgtaaaacc ataaaagata gagacactga
    20041 gagtaagaag aaagcaattt gtgttataaa ggatgctacg aaaaaagctg aatcactgcg
    20101 tatagatgct gtatgcgatg gttatcaaat tggtatacag actgcttttg agcatattat
    20161 tgactatatt tgtgaatgga agttaaagca aaatgaaaac agaagaaata ttgaggacta
    20221 tataactagt ttgctttcag aaaatttaca tgatgagagg atcattagca ctttattgga
    20281 gcagtggctt agttcgttgc gtaatacagt aacagaatta aaagtggttt tgcccaagtg
    20341 taatttagca ctgaggaaaa agttagaact tgatctccat aaatatcgct cagatgtcaa
    20401 gattatttta aaatattctg aagggaataa ttatatattc tgtagtggta atcaagtagt
    20461 ggagtttagt cctcaggatg taatatcagg tgttaaaata gaacttgcgg aaaaattaac
    20521 aaaaaatgat aaaaaatatt tcaaagagtt agcacataag aaactgagac aaattgcaga
    20581 agacttgtta aaagagaatc ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc
    20641 agcaaagatt aaatagtgaa gaatttgtta atttaaatga gagattatca tcttcgcaat
    20701 catttgatga gaatataatc tatgaaataa tgcaatattt ctcacagagt gaattaaatt
    20761 ccatagataa tgatgaactg cacaataaga ttgaacaatt attcaattca cgttttcctt
    20821 atcttacagc tgcacaaaaa agttcacttc ttaataaact catagatgca aatcaatatg
    20881 tcgatttgca tgagggattt tatgcatcat tgtccatata taacaatatt gatttctata
    20941 ttaagactac aaccttcgat tcattaatta gtgtctttga agcagggaga gaggcagatg
    21001 attcgacatg gtagtaataa gttgaaaata tttattttaa gtatattgct attaacactg
    21061 agtgggtgtg ctttaaagtc atcatctaat tctgaaaaag aatggcatat tgttcctgta
    21121 agtaaggatt atttttctat tccaaatgat ttattatggt cgtttaatac aaccaataaa
    21181 agtataaatg tttactctaa atgtattagt ggtaaggcgg tttatagttt taatgcaggt
    21241 aaattcatgg gcaactttaa tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag
    21301 atagctatag ataaactatt ttctatgctg aaagacgggg ttgttttaaa aggtaataag
    21361 ataaatgata ccatccttat agagaaggat ggggaagtta aattaaaatt aattcgaggg
    21421 atataattgt attgtgagta aatataaagg tctaaataca agtaatatgt tttacattta
    21481 ctctagtgga catgaaccag ttaacgttga gcttgtaaaa gataaagaac gtaacataat
    21541 tgagctggct ccagcatgga agggtttttt tttgtccgaa atcaaaatat aaaattcagt
    21601 gataatgtga actatcatta tcgctttaac attaatagtt gtgcaaaatt tcttgctttt
    21661 tgggactatt tcagtggtgc tctagtagag catagccatg ctgaaaaatg tattcatttc
    21721 tatcatgaga atgatcttcg ggatagttgt aataccgaat cgatgcttga taagttaatg
    21781 ttaagattca tcttttcatc tgaccagaat gtaagtaatg cactggctat gatacgaatg
    21841 actgagtcat atcatttggt tctttatctt ctgagaacta ttgagaaaga aaaggaggta
    21901 agaataaaga gtctgacaga gcattatggt gtgtcggagg cttattttag atctttatgt
    21961 agaaaagctt taggggcaaa agtcaaagaa caactgaaca cttggcggtt agtaaatggt
    22021 ttattagatg tatttttgca caatcaaaca atcacttctg ctgccatgaa taatgggtat
    22081 gcatcaacat cccatttttc aaatgagata aaaacacgac taggcttttc tgcaagagaa
    22141 ttatcgaata ttactttttt agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt
    22201 gactctcttg attgtattgt taccattgat agttaatgct aataatattg actctcattt
    22261 attagaacaa aatgacatag cgaaatatgt tgctcaaagt gatacggtag gatctttttt
    22321 tgaacgtttt tctgcgttat taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa
    22381 gagaatatct ggggagtttg atttatctaa tccagaagaa atgcttgaaa aaatgacatt
    22441 acttgtggga ctcatatggt ataaagatgg aaatgcatta tacatatatg actctggaga
    22501 attaataagc aaagttattt tgctggagaa tatatcactt aattatctta ttcagtatct
    22561 gaaggatgct aacctttatg atcacagata tcctataaga ggaaacatta gtgataaaac
    22621 attctatata tctggtcctc cggcattggt tgaactggta gctaatacag caactttgct
    22681 tgataagcag gtttcttcta ttgggacaga taaagttaat tttggggtta taaaattaaa
    22741 aaatacattt gttagtgaca gaacttataa tatgagaggg gaggatattg tcatcccagg
    22801 ggtggctact gttgtagaac gtttattaaa taatggtaag gctctgagta acagacaagc
    22861 caaaaatgat ccgatgccac cctttaatat tacacagaaa gtatctgagg atagtaatga
    22921 cttttcattt agttcagtta ccaatagttc tatattagag gatgtatcac ttattgcata
    22981 tccagaaacc aattctatat tagtcaaagg taatgatcag caaatacaga ttattcgcga
    23041 tattatcact cagttagatg tagccaaacg acatatagaa ttatcattat ggataatcga
    23101 tattgataaa tctgaattaa ataaccttgg cgttaattgg caaggaacgg cttcatttgg
    23161 tgattcgttt ggtgcatcat ttaatatgag ctcatcagca tcgataagca ctttggacgg
    23221 caataagttt attgcttcag ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc
    23281 aaggccggtg attttgacac aagagaatat tccggctata tttgataata acaggacttt
    23341 ttatgtgtca ttagttggtg agcgcaattc ttcgttggag catgttacgt atggaacatt
    23401 gataaatgtt atacctcgct tttcatctca gggacaaatt gagatgtctt taacgataga
    23461 ggatggaaca ggaaatagtc agtcaaatta caattataat aatgaaaata catctgtcct
    23521 tccagaagtt ggaaggacca aaatcagtac aatagcgaga gttccacaag gcaaaagttt
    23581 gttgataggt ggttatacgc atgaaacgaa ctctaacgaa atagtatcta ttccttttct
    23641 ctcctctatt cctgtaatcg gtaatgtgtt caagtataaa acaaggaata tcagtaatat
    23701 tgtaagggtt tttctgattc aaccccgtga gataaaggag tcaagttatt ataatactgc
    23761 agaatataag agcctcatat ctgaaagaga aatacaaaag actacacaaa taatcccatc
    23821 tgaaactact ttgctggaag acgaaaaatc attggtttca tacttaaatt actaactata
    23881 aagtaggtga tgtatgcttg atgttaaaaa tacaggagtt tttagctctg cattcattga
    23941 taagttgaat gcaatgacaa attcagatga tggagatgag actgctgatg cagagcttga
    24001 ttctggcttg gctaatagca agtatattga ctcatctgat gagatggctt ccgctctttc
    24061 gtcatttata aacagaagag accttgagaa actgaaagga acaaatagtg atagtcagga
    24121 acgtatttta gatggggaag aagatgaaat taatcacaag atttttgatt taaagagaac
    24181 tttaaaagat aaccttcctc tagatcagga ttttatagac agactaaaga gatattttaa
    24241 agatccaagt gatcaagtct tagcattaag ggaacttttg aatgaaaaag atcttactgc
    24301 tgaacaagtc gaatcattaa ctaaaattat taatgagata atatcaggta gtgaaaaaag
    24361 tgttaatgct ggaataaatt cagctataca ggctaaatta tttggcaaca aaatgaaact
    24421 tgaaccacag cttttgcgtg catgttatcg tggttttatc atggggaaca tatcaacaac
    24481 agatcagtat atagaatggc ttggtaattt tggttttaat cacagacata caattgtgaa
    24541 ttttgtagag cagtcactga ttgtagacat ggattctgag aaaccgagct gtaatgctta
    24601 tgagtttggt tttgtgttat ctaaattaat tgcaattaag atgattagaa cttcagatgt
    24661 aatttttatg aagaaactgg aatcctcaag cttgctaaaa gatggcagtt taagtgcaga
    24721 gcagctattg ctaactttat tatatatttt tcaatatcca agtgaaagtg agcaaattct
    24781 tacttctgtt atagacgtat cacgagccag tcatgaggat tctgtagtgt atcaaacata
    24841 tctatcttct gttaatgaaa gtcctcatga tatatttaaa agtgaaagtg aaagagaaat
    24901 tgcgatcaat attctacgag agcttgtcac aagtgcatac aagaaagagc tttctagata
    24961 acaggagatg aaagtgatcc agtcttttct taagcaagta agtactaagc ctgaattaat
    25021 tatattagtg cttatggtta tgatcattgc tatgttgatc ataccattac ctacatacct
    25081 tgttgatttt ttaatagggc ttaatattgt acttgctatt ctcgttttta tgggatcatt
    25141 ttatattgaa agaatattga gtttttctac attcccttcg gttttgttga taacgacatt
    25201 atttcgtctc gctttatcaa taagtactag tcgactaatt ttagttgatg cggatgcggg
    25261 taaaattatt actacatttg gccagtttgt cattggtgat agtctggctg taggttttgt
    25321 tattttttca atagtaactg ttgtgcagtt cattgttata acaaaaggat ctgaaagggt
    25381 tgcggaggta gctgctcgct tttctcttga cggtatgccg gggaaacaaa tgagcattga
    25441 tgcggattta aaggctggaa ttattgatgc cgcaggagct aaagaaagac gcagtattct
    25501 ggaacgtgag agtcaattgt atggttcatt tgatggagca atgaagttca ttaaaggtga
    25561 tgcaattgct ggcatcatta ttatttttgt caatttaata ggaggtattt ctgttggtat
    25621 gagtcaacat ggaatgtctc tatctggtgc attatctact tataccatac taactattgg
    25681 tgacggattg gtctctcaga ttcctgctct gttaatttct ataagtgcag gatttatcgt
    25741 tacaagagtt aatggtgata gcgataatat gggacgtaac ataatgtccc agatatttgg
    25801 gaatcctttt gttctcattg ttacatcagc tcttgccttg gcgataggga tgttgccagg
    25861 ttttccgttt tttgtttttt tcctgatagc agttactttg acggctttat tctattataa
    25921 aaaggtcgta gaaaaagaaa aaagtctgtc tgagtctgat tctagtggct atactggtac
    25981 atttgatatt gataatagcc atgactcatc tttggcaatg atagaaaatc tggatgctat
    26041 tagttcagaa accgttcctt taattttatt atttgccgaa aataagataa gtgcaaatga
    26101 tatggaaggt cttattgaaa ggataagaag tcagttcttt attgattatg gtgtcaggct
    26161 tccaactatt ttatatagaa caagcaatga gcttaaggtc gatgatattg ttttgttaat
    26221 aaatgaggtg cgtgccgata gttttaatat atactttgac aaagtgtgta ttacagatga
    26281 aaatggagat atagatgctc taggtattcc tgttgtttca acttcatata atgaacgtgt
    26341 tatttcttgg gtagacgttt catatacaga aaatctaact aatattgatg ctaaaattaa
    26401 aagtgctcaa gatgaatttt atcaccagtt gtcacaagct ttattaaaca acataaatga
    26461 gatttttggt atacaagaaa caaaaaatat gttagatcag tttgaaaatc ggtatcctga
    26521 tctattaaag gaagtcttcc gacatgtgac tatacagaga atttctgagg tattacaaag
    26581 attgcttgga gaaaatattt ctgttcgcaa tttaaaactt attatggagt ctttggcgct
    26641 ttgggctccc agagaaaaag atgtcataac attagttgaa catgtccgtg catcactttc
    26701 taggtatatt tgtagtaaaa tagctgtttc tggtgagatt aaagttgtga tgctttccgg
    26761 atatattgag gatgcaataa gaaaagggat aaggcaaacc tctggtggct ctttcttgaa
    26821 tatggatata gaggtttcgg atgaggtaat ggaaacttta gcacatgctt tgagagaatt
    26881 gagaaatgca aaaaaaaatt tcgttctttt ggtatcagta gatatacgta ggtttgttaa
    26941 aagacttata gataacagat ttaagagtat actcgttata tcgtatgctg agattgatga
    27001 agcatatacc attaatgtat taaagactat ttagtgaggt ttaaatatga gtaacattaa
    27061 tttagttcaa ttagttagag atagtctttt cacgattggt tgtcctccat caattattac
    27121 agatttagac agccattcag cgattaccat ttctttggat agtatgcctg ctattaatat
    27181 agctcttgtc aatgagcagg ttatgttatg ggctaatttc gatgcaccaa gtgatgtaaa
    27241 acttcagtca tctgcttata acatattaaa tttaatgtta atgaacttta gttatagcat
    27301 taatgagctg gtagagcttc atcgttctga tgagtactta cagttaagag ttgttattaa
    27361 agatgactac gttcacgatg ggattgtttt tgcagagatt ctgcatgagt tttatcagag
    27421 gatggaaatc ttaaatgagg tcttataatc aatgagctat ataaaattgc tcactcaatt
    27481 atcttttcct aatagaatct cggggccaat cttggaaaca agtcttagcg atgtttcgat
    27541 tggtgagatt tgtaacattc aggctggaat tgaaagtaat gaaattattg caagagctca
    27601 ggttgtagga tttcatgatg aaaaaacaat attaagcttg attggaaatt ctcgtggact
    27661 ttcaaggcaa acgttgatta agcccactgc ccagtttctt catacgcaag tcggccgtgg
    27721 attattggga gcagtagtca atcctttagg ggaggttact gataagtttg ctgttacgga
    27781 taacagtgaa attctttatc gacctgtaga taatgctcct ccgctatata gtgaaagggc
    27841 tgcaattgag aagccttttt taacaggtgt taaggttatt gattctttac tcacgtgtgg
    27901 tgaaggacag cgaatgggga tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa
    27961 tatgctcatt gaacatagtg gtgctgatat atatgttatt gggttaattg gtgagcgagg
    28021 tcgagaggtt acagaaacgg ttgattattt gaagaactct gagaaaaaaa gcaggtgtgt
    28081 tttagtatat gcaacttcgg attactcttc ggttgatcgt tgtaatgctg catatatagc
    28141 cactgctata gccgaatttt ttaggactga aggacataaa gtagcgcttt ttattgattc
    28201 attaacaagg tatgccagag cattacgtga tgtggcctta gccgctggag aatcacctgc
    28261 cagaagaggc tatccggttt cggtttttga tagcttaccc agacttcttg aaaggccagg
    28321 aaagttaaag gcaggtggct ctattactgc attttacact gttcttttgg aggatgatga
    28381 ttttgctgat ccattagctg aagaggtaag atccatttta gatggacata tatatttgag
    28441 cagaaatcta gcccaaaaag gacaatttcc tgcaattgat tccttaaaaa gtataagcag
    28501 ggtatttaca caggttgttg atgaaaaaca tcgtattatg gccactgcat ttcgggagct
    28561 actttctgaa atagaagagc taaggacaat tattgacttt ggtgaataca aaccggggga
    28621 gaatgcctct caggataaaa tctacaacaa aatatctgtt gttgagagtt ttctgaagca
    28681 agattatcga ctcggtttta cttatgagca gacaatggag cttcttggtg aaacaattag
    28741 ataaggtttt aaaaatcaaa gataaatatc aacggtctgt taaattaatt gaagcacaca
    28801 tattaacact aaaaaaaaaa ctctttgtat cgagatgtgg aggcattaga taaaaggatt
    28861 atatacttcc tgcaactgga gaatgacttg gaacctgtag gcgctcagag tgttagtcag
    28921 cttttcaata caagacgtaa gatcgctata gtaaaaaagc atattatcca atatcagtct
    28981 gaacgaattc ttctaaaggg aagaattgaa gaaattcaaa aagatattga tgaggcaaat
    29041 gcttctaaaa gaaaactgct tcataaagag agtaaaatat gtaaacggat tggcttgatt
    29101 aaaagaaata actttgccaa acagttgata cttgatgagt tatcacaaga ggatatgaag
    29161 tatggcatta gataatataa acctaaattt tagtagtgac aaacagatag aaaaatgtga
    29221 gaaactatct tcaatagata atattgatag tctcgttttg aagaagaaga ggaaggttga
    29281 aattcctgag tactctttaa ttgcatctaa ctattttact attgataagc actttgaaca
    29341 taagcatgat aaaggagaaa tttatagtgg cattaaaaat gcgttcgaac ttagaaacga
    29401 acgagcgaca tattctgata ttccagaatc aatggccatt aaagaaaata ttttgatacc
    29461 agatcaagat atcaaagcaa gggaaaaaat aaatatcggc gatatgaggg ggatcttttc
    29521 atataataag agtggaaatg cagacaagaa cttcgaaaga agtcatactt cttctgtaaa
    29581 ccctgataat ctgctagaat ctgataatag aaatggtcaa attggtttaa aaaatcatag
    29641 cttgtctatt gataagaata ttgctgacat catttcttta ctaaatggaa gtgttgctaa
    29701 atcatttgag ctgcctgtaa tgaataaaaa tactgcagac ataaccccat ccatgtcatt
    29761 gcaagaaaaa tcaatagttg aaaatgataa aaatgttttt caaaaaaata gtgaaatgac
    29821 ttaccacttt aaacagtggg gggctggaca ttctgttagt atttcagtgg agtctggttc
    29881 ttttgttcta aaaccgtcag atcaatttgt aggaaataaa cttgacttaa ttttgaaaca
    29941 agatgctgag ggtaattaca gatttgatag cagtcaacat aataagggga ataaaaataa
    30001 tagtacagga tataatgaac agagtgaaga agaatgctaa gaattaaaca ttttgacgct
    30061 aacgaaaaac tacagatttt atatgcaaag caactctgcg agcgtttttc gattcagaca
    30121 ttcaaaaata aatttacagg cagtgaaagt ttagtcactc ttacttccgt gtgtggggat
    30181 tgggtaattc gtattgatac attatctttt ttgaaaaaaa aatacgaggt attttcagga
    30241 ttttctacac aagaatcttt actgcattta tcaaaatgtg tctttataga gtcgtcatct
    30301 gtattttcga ttccagaact gtctgataag attactttcc ggatcacgaa tgaaatccag
    30361 tatgcaacta ctgggagtca tttatgctgt ttttcctctt ctttaggtat tatttatttt
    30421 gacaagatgc cggtattacg taatcaagtt tctcttgact tattgcatca tcttttagag
    30481 ttttgcttag gttcatctaa tgtaaggctg gctactttaa aaagaattcg tactggtgat
    30541 ataatcatag ttcagaaact ttataattta ttattgtgta atcaagttat tattggggat
    30601 tatattgtga atgataataa tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca
    30661 gatcacacag aagtttcttt ggcattattc aattatgatg atatcaatgt aaaagtggac
    30721 tttatccttt tagaaaaaaa gatgacaatc aatgaactaa aaatgtatgt agaaaacgaa
    30781 ttattcaagt ttcccgatga catagttaaa catgtaaata ttaaagtaaa tggttctttg
    30841 gttgggcatg gggaacttgt ttctattgag gatggttatg gtatcgagat tagttcttgg
    30901 atggtaaagg agtaatctca tgctgagtga tatgtccctc atcgctacac tttctttttt
    30961 tacgctatta ccatttttag ttgctgctgg tacttgttat ataaagtttt ctattgtttt
    31021 tgtaatggta cgaaatgcac ttggtttgca gcaggttcca tcaaatatga ctctcaatgg
    31081 tattgcattg attatggctt tgttcgtgat gaaacccatt attgaagctg gttatgaaaa
    31141 ttatctaaat ggcccgcaaa aatttgatac aatatcagat attgtccgtt tttctgattc
    31201 tggcttgatt gaatacaagc aatacttgaa aaagcataca gatcttgagt tggcacgttt
    31261 tttccaaagg tctgaagaag aaaatgctga tttaaagagt gcagagaata atgattattc
    31321 attgttttcc ctcttgcctg catatgcgct aagtgaaatt aaagatgcgt tcaaaatagg
    31381 tttttattta tatttgccat ttattgttgt tgatcttgtt atttcaagca ttttacttgc
    31441 acttggtatg atgatgatga gtccaatcac aatatcagta ccaataaaac ttgtattatt
    31501 tgttgcgctt gatgggtggg ggatcctgtc taaggccttg attgaacaat atatcaatgt
    31561 tcctgcttag atgatggagg taatgtgtct gatatagttt atatgggtaa taaggctctt
    31621 tatttaatcc ttatcttttc cttatggcca gtaggtatag ctacggttat tggattaact
    31681 attggtttat tacagacagt gactcaactt caagagcaga cacttccttt tggtataaag
    31741 cttataggtg tctcaatatc tttgctactt ctttctggat ggtatggtga ggttttattg
    31801 tctttttgtc atgaaataat gtttttaatt aagagtgggg tttgatggac atttcaagct
    31861 ggttcgaaag tattcatgtg tttttaatac tcctgaacgg cgtttttttt agattggctc
    31921 cattattttt ctttcttcca tttttaaata acggtataat ttctccatct attagaatac
    31981 ctgtgatttt tcttgttgca tcaggattaa ttacttctgg taaggtagac ataggttctt
    32041 ctgtttttga acatgtttat ttccttatgt tcaaggaaat aattgttggc ctccttctct
    32101 ctttttgctt gtctcttccc ttttggatat ttcatgctgt tggtagtatt attgacaacc
    32161 agcgtggggc aacgcttagt agttcaattg atcctgccaa tggtgttgat acgtctgaat
    32221 tggcaaaatt tttcaatctt ttttctgcag ttgtatttct atacagtggt ggaatggtct
    32281 ttattttaga atccatacaa ttgtcttata atatatgccc gttattttct caatgttctt
    32341 tccgtgtctc aaatatctta acatttctga ctttattggc aagtcaggct gttattttag
    32401 ccagtcctgt tatgatagta ttgttactat cagaagtatt acttggtgta ttatcgagat
    32461 ttgctccgca gatgaatgct ttttccgtat cattaactat taaaagttta cttgcaatat
    32521 ttattatttt catctgttct tctactattt acttttctaa agttcaattt ttcctcggtg
    32581 aacataaatt tttcacaaat ctatttgtta gataaaatat tatggcaaat aaaacagaaa
    32641 agccgacacc taaaaaacta aaggatgccg caaaaaaagg acagtcattt aaatttaagg
    32701 atttaacgac agttgttatt attctggtag ggacatttac tataatatca ttcttttcct
    32761 taagtgatgt aatgctctta tacagatatg taataattaa tgacttcgaa attaatgagg
    32821 gtaaatactt ttttgcagtg gttattgtct tttttaagat aattggcttc ccactttttt
    32881 tctgtgttct ttcggctgtg ttgccaacat tggttcaaac aaagtttgtt cttgcgacta
    32941 aagctatcaa gattgatttt tcagtattaa accctgttaa agggttaaaa aaaatattta
    33001 gtataaagac aataaaagaa tttttcaaaa gcattctgct tcttattatt ctagcattaa
    33061 caacctattt cttttggatt aatgaccgaa aaataatttt ttctcaggtg ttttctagtg
    33121 ttgatggctt atatcttatt tggggggggc tatttaagga tataatatta tttttcttag
    33181 cattttctat ttttgttatt attcttgact ttgtgattga gttcatttta tacatgaaag
    33241 atatgatgat ggataaacag gagataaaaa gagaatatat agagcaagag ggacactttg
    33301 agacaaagtc gagaaggcgt gagttgcata tcgagattct ttcagagcag actaaatctg
    33361 atatacgtaa ttcaaaatta gtggtaatga acccgactca tattgcaatt ggtatttatt
    33421 ttaatccaga aatagcgcct gcacctttta tttctctcat tgaaactaac cagtgtgcct
    33481 tggctgtcag aaaatatgca aatgaagttg gtataccaac tgtgcgtgat gtgaaattag
    33541 ctagaaaact atataaaaca catacaaaat atagttttgt tgattttgaa cacttggatg
    33601 aagtcctacg tcttattgtt tggcttgagc aggttgaaaa cactcattaa taagtaaagg
    33661 agatgtgtta tatgggagtt aatttctgta ataaaatagg tattgatcag tctgaatttg
    33721 aaatagaatc ttccatcatt aactccattg ctaatgaggt attgaaccca atatctttcc
    33781 ttagcaacaa ggatataata aatgttttac tcagaaagat ttcatctgaa tgtgacctgg
    33841 ttagaaaaga catttatcgt tgtgctcttg agttggtcgt tgaaaaaaca cctgatgatt
    33901 tataatcatt cataataact cttttgttga tataaatatt tttgttgaag atatttatgt
    33961 agtcacagga tcaattgtag aaaattgtta tgtttttgtt aggaactgaa ctaaataata
    34021 tatgataaga cagcaaaaac gactgaccat aatccttctt ttattaggag ttgataagag
    34081 agactattca tcctgtaatg ttaaaactct gttatatagt attcgcgatt atgcaaagtc
    34141 tgtaaatgat catgagatac taaccgagag taatcgttta ttatctcatt gcatttcaga
    34201 tagtaacgga gctttcttta aaagtagtaa atacgttccc cttaagtact tacgtaaaag
    34261 gagaatagca cgaaagatac caaatgatta gcactttgct agtaagcttg ctggtaaact
    34321 atattgaccg ttgggtttgt tttgagactg taatttaaat tgtgtaattg cctgtttttg
    34381 atatgtttac tccaacaacg gagacaggta aattatggac gaaaagaagc ttaaagccct
    34441 tgcggctgaa ctggctaaag gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat
    34501 gctgacgaaa tatcccggct cttaaaacga tagtggctca ggctttcatt tttcagcgtt
    34561 ccccagaag
//
LOCUS       DAJTYD010000026        34001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTYD010000026 DAJTYD010000000
VERSION     DAJTYD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.026"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(152..364)
                     /gene="rpmE"
                     /locus_tag="OO585_001113"
     CDS             complement(152..364)
                     /gene="rpmE"
                     /locus_tag="OO585_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6628770.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            567..2765
                     /gene="priA"
                     /locus_tag="OO585_001114"
     CDS             567..2765
                     /gene="priA"
                     /locus_tag="OO585_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6628771.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2921..3946
                     /gene="cytR"
                     /locus_tag="OO585_001115"
     CDS             2921..3946
                     /gene="cytR"
                     /locus_tag="OO585_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6628772.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4038..4997
                     /gene="ftsN"
                     /locus_tag="OO585_001116"
     CDS             4038..4997
                     /gene="ftsN"
                     /locus_tag="OO585_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6628773.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHNKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5090..5620
                     /gene="hslV"
                     /locus_tag="OO585_001117"
     CDS             5090..5620
                     /gene="hslV"
                     /locus_tag="OO585_001117"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6628774.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5630..6961
                     /gene="hslU"
                     /locus_tag="OO585_001118"
     CDS             5630..6961
                     /gene="hslU"
                     /locus_tag="OO585_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6628775.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7028..7954
                     /gene="menA"
                     /locus_tag="OO585_001119"
     CDS             7028..7954
                     /gene="menA"
                     /locus_tag="OO585_001119"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6628776.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8047..8532
                     /gene="rraA"
                     /locus_tag="OO585_001120"
     CDS             8047..8532
                     /gene="rraA"
                     /locus_tag="OO585_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6628777.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8617..8862)
                     /gene="zapB"
                     /locus_tag="OO585_001121"
     CDS             complement(8617..8862)
                     /gene="zapB"
                     /locus_tag="OO585_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6628778.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9288..10133
                     /gene="glpF"
                     /locus_tag="OO585_001122"
     CDS             9288..10133
                     /gene="glpF"
                     /locus_tag="OO585_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6628779.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10156..11664
                     /gene="glpK"
                     /locus_tag="OO585_001123"
     CDS             10156..11664
                     /gene="glpK"
                     /locus_tag="OO585_001123"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6628780.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRSE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11799..12809
                     /gene="glpX"
                     /locus_tag="OO585_001124"
     CDS             11799..12809
                     /gene="glpX"
                     /locus_tag="OO585_001124"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6628781.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12906..13652
                     /gene="fpr"
                     /locus_tag="OO585_001125"
     CDS             12906..13652
                     /gene="fpr"
                     /locus_tag="OO585_001125"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6628782.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13657..14085)
                     /gene="uspD"
                     /locus_tag="OO585_001126"
     CDS             complement(13657..14085)
                     /gene="uspD"
                     /locus_tag="OO585_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6628783.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14112..14411)
                     /locus_tag="OO585_001127"
     CDS             complement(14112..14411)
                     /locus_tag="OO585_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6628784.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSVDEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14623..15063)
                     /locus_tag="OO585_001128"
     CDS             complement(14623..15063)
                     /locus_tag="OO585_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6628785.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15164..15763
                     /locus_tag="OO585_001129"
     CDS             15164..15763
                     /locus_tag="OO585_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6628786.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15871..16638
                     /gene="tpiA"
                     /locus_tag="OO585_001130"
     CDS             15871..16638
                     /gene="tpiA"
                     /locus_tag="OO585_001130"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6628787.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16693..17448)
                     /gene="cdh"
                     /locus_tag="OO585_001131"
     CDS             complement(16693..17448)
                     /gene="cdh"
                     /locus_tag="OO585_001131"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6628788.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17555..18544)
                     /gene="sbp"
                     /locus_tag="OO585_001132"
     CDS             complement(17555..18544)
                     /gene="sbp"
                     /locus_tag="OO585_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6628789.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18864..19826)
                     /gene="pfkA"
                     /locus_tag="OO585_001133"
     CDS             complement(18864..19826)
                     /gene="pfkA"
                     /locus_tag="OO585_001133"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6628790.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20007..20909)
                     /gene="fieF"
                     /locus_tag="OO585_001134"
     CDS             complement(20007..20909)
                     /gene="fieF"
                     /locus_tag="OO585_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6628791.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21057..21569)
                     /gene="cpxP"
                     /locus_tag="OO585_001135"
     CDS             complement(21057..21569)
                     /gene="cpxP"
                     /locus_tag="OO585_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6628792.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21719..22417
                     /gene="cpxR"
                     /locus_tag="OO585_001136"
     CDS             21719..22417
                     /gene="cpxR"
                     /locus_tag="OO585_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6628793.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22414..23787
                     /gene="cpxA"
                     /locus_tag="OO585_001137"
     CDS             22414..23787
                     /gene="cpxA"
                     /locus_tag="OO585_001137"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6628794.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23893..24567)
                     /gene="yiiM"
                     /locus_tag="OO585_001138"
     CDS             complement(23893..24567)
                     /gene="yiiM"
                     /locus_tag="OO585_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6628795.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24716..25681)
                     /gene="kdgT"
                     /locus_tag="OO585_001139"
     CDS             complement(24716..25681)
                     /gene="kdgT"
                     /locus_tag="OO585_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6628796.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25960..26580)
                     /gene="sodA"
                     /locus_tag="OO585_001140"
     CDS             complement(25960..26580)
                     /gene="sodA"
                     /locus_tag="OO585_001140"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6628797.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26864..27898
                     /gene="rhaT"
                     /locus_tag="OO585_001141"
     CDS             26864..27898
                     /gene="rhaT"
                     /locus_tag="OO585_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6628798.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27895..28761)
                     /gene="rhaR"
                     /locus_tag="OO585_001142"
     CDS             complement(27895..28761)
                     /gene="rhaR"
                     /locus_tag="OO585_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6628799.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28817..29653)
                     /gene="rhaS"
                     /locus_tag="OO585_001143"
     CDS             complement(28817..29653)
                     /gene="rhaS"
                     /locus_tag="OO585_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6628800.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29941..31353
                     /gene="rhaB"
                     /locus_tag="OO585_001144"
     CDS             29941..31353
                     /gene="rhaB"
                     /locus_tag="OO585_001144"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6628801.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGLDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31350..32609
                     /gene="rhaA"
                     /locus_tag="OO585_001145"
     CDS             31350..32609
                     /gene="rhaA"
                     /locus_tag="OO585_001145"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6628802.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDQVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32876..33700
                     /gene="rhaD"
                     /locus_tag="OO585_001146"
     CDS             32876..33700
                     /gene="rhaD"
                     /locus_tag="OO585_001146"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6628803.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33710..>34001
                     /gene="rhaM"
                     /locus_tag="OO585_001147"
     CDS             33710..>34001
                     /gene="rhaM"
                     /locus_tag="OO585_001147"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6628804.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASADVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8338 a         8857 c         8822 g         7984 t
ORIGIN      
        1 ccttttgaaa taaataagaa ccagaagcct agcgccagtc aacaacagga aatgataatg
       61 agttaacacc ccggaatttt tcagaattta taaaaggcaa aattcaggca caaaaaaagc
      121 gccgtgcggc gcttttttcg gaaatccggt cttatttgct gcccgggatg ttgaaacgct
      181 tgttgaagcg gtcaacacgg ccaccggtag caacatcacg ctgtttgcca gtgaagaacg
      241 ggtggcactt gctgcacacg tcgaggttca ggtcatgacc aacagtggag cggattttca
      301 ttacgttacc gcaagagcag ctagcagtaa tttcttcgta tttcgggtga atatcttttt
      361 tcatgggaaa acctcggttt aaggccgcgt cgctcttcca gccctaacgc cagacaccac
      421 gcgatgttaa aagtatagct tcaatacgat catttcgtac gaaggcgcga aatcatacag
      481 aaattaacca acgtatgcaa actgatccgc actcttctac ggcaatgtgt atactaaccc
      541 accgaatttc aagtcaggat gatgctatgc ccgttgccca cgttgccttg cccgttccgc
      601 ttcctcgtac ctttgactat ctgctgccag aaggcatgac ggttaaagcc gggtgtcgcg
      661 tgcgcgtgcc gtttggcaaa cagcaggagc gcatcggggt tgtggtgtca gttagcgatg
      721 tcagtgagct gccgcttaac gagctgaaag cggtagtgga agtgctggac gttgagccgg
      781 tatttactca ctccgtctgg cggttactgc tctgggcggc agattactat catcatccga
      841 ttggcgatgt gctgtttcat gccttgccga ttttactgcg ccaggggcgg cctgcggcga
      901 acgcgccgat gtggtactgg tttgccactg aacaaggcca ggcggtggat ctgaacagcc
      961 tgaaacgctc ccccaagcaa caacaggcgc tggcggcgtt acggcaaggc aaaatctggc
     1021 gcgaccaggt cgccacgctc gaatttaatg atgccgcgtt gcaggcgcta cgcaaaaaag
     1081 gtctgtgtga tttagcaagt gaaacaccag agtttagcga ctggcgaacg aactatgccg
     1141 tttctggtga gcggttgcga ttgaataccg aacaggccac cgccgttggc gcaattcata
     1201 gcgcggcaga tactttttct gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg
     1261 aggtttatct cagtgtactg gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg
     1321 tgccggaaat cggcctgaca ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc
     1381 ccgtggaagt tctgcattcc ggcctgaacg acagcgagcg gctttctgcg tggttgaaag
     1441 cgaaaaatgg cgaggcggcg attgtgatcg gcacccgctc cgcgctgttt acgccgttta
     1501 aaaatctcgg cgtgattgtc attgatgaag agcacgacag ctcctacaag cagcaggaag
     1561 gctggcgcta tcatgcccgc gacctggcgg tgtatcgtgc gcacagcgag caaatcccga
     1621 ttattcttgg ctccgcaacg cccgcgctgg aaacgttatg caacgtccag cagaaaaaat
     1681 accgcctgct gcgcctgacc cgtcgggcag ggaatgctcg tccggcaatt caacatgtgc
     1741 tggatttaaa aggtcagaag gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc
     1801 gccagcattt acaggccaac aatcaggtta tcctctttct taatcgtcgg ggttttgcgc
     1861 ctgcgctgct gtgccacgac tgcggctgga tagccgaatg cccacgttgc gatcactact
     1921 acacgctgca tcaggcgcaa cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg
     1981 tgccgcgcca gtgcccttcc tgcggttcca cgcacctggt ccccgtgggg ctgggcaccg
     2041 aacagcttga acagacgctt gcgccgatgt tccccggcgt gcccatttca cgtatcgacc
     2101 gcgataccac cagccgcaaa ggggcactgg aacagcaact ggcggaagtg catcgcggcg
     2161 gcgcgaggat tttgatcggc acacaaatgc tggcgaaagg tcaccacttt ccggatgtga
     2221 cgctggtggc gttactggat gtggacggcg cgctgttttc tgccgatttt cgctcggcag
     2281 aacgtttcgc ccagctttac acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg
     2341 gcgaagtggt gctgcaaacg caccatccgg aacatcctct gttgcaaacg ttgctctata
     2401 aaggctacga cgcctttgcc gaacaggcgc tggctgagcg acgcatgatg cagctgccgc
     2461 cgtggaccag ccatgtgatt gtgcgtgcgg aagatcataa caatcagcac gcgccgctgt
     2521 tcctgcaaca actgcgtaat ctgatccttt ccagcccgct ggcagacgac aaactgtgga
     2581 ttctcggccc ggttccggct ctggcaccta agcgtggtgg ccgctggcgc tggcagatct
     2641 tgttgcagca cccttcccgc gtgcgcttgc aacacatcat tagtggtacg ctggcgctta
     2701 ttaatacaat accggattcc cgcaaggtga aatgggtgtt ggatgtcgat cccatcgaag
     2761 gctaatgcgc aaacgttgcg aggcggatcg aaaaattcaa tattcatcac acttttcatg
     2821 aaaattctgt aaccgttttc acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac
     2881 atggatgtag tacgcctgac gtaccaggcg aggagtgagt gtgaaagcga agaagcagga
     2941 aactgccgcg accatgaaag acgttgccct caaggcaaaa gtctctacag cgactgtctc
     3001 ccgagcatta atgaatcccg ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc
     3061 ggcccgggaa gtgggttatt taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc
     3121 ccgcaccatt ctggtgattg tcccggatat ctgcgatccc ttctttagcg aaattattcg
     3181 cggtatcgaa gttacggcgg caaatcacgg atatctggtg ctgattggcg actgtgcgca
     3241 tcaaaatcag caggaaaaaa cctttatcga tttgatcatc accaagcaaa ttgatggcat
     3301 gttgctgctg ggttcaaggc tgccgtttga tgccagcatt gaggaacagc gtaatctgcc
     3361 gccgatggtg atggcgaacg aatttgcacc ggaactggag ctgcctaccg ttcatatcga
     3421 caatctgacc gccgcatttg atgcagtaaa ttatttatat gagcaagggc ataaacggat
     3481 tggctgtata gccggtcccg aagagatgcc actgtgtcac taccgcctgc aaggctatgt
     3541 tcaggcgctg cgtcgctgcg gaattatggt tgatccacaa tacatcgccc gtggcgactt
     3601 caccttcgaa gccggaagca aagcaatgca acagctgctt gatcttccac aaccgcctac
     3661 tgcagtcttc tgccatagcg atgtgatggc gctcggcgca ctttctcagg caaaacgcca
     3721 ggggctgaaa gtcccggaag acctttccat aatcggtttt gataacatcg acctgacgca
     3781 attttgtgat ccgccgctga caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc
     3841 tatgctgtta ttgcttgatc aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat
     3901 ggactgcgaa cttatcatcc ggggatcaac acgcgcgtta ccttaaagta aacctttaag
     3961 actgccgtga ctggtcaaag gcccgccgct taagtaacat ggcgggctga cgaacgaata
     4021 aatacagcga aacgatagtg gcacaacgag attatgtacg ccgcagccaa ccggcacctt
     4081 cgcggcgaaa aaagagcacc tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg
     4141 ctatggtcgc tattgctgcc gccgttcttg tgacctttat cggtggtctg tacttcatta
     4201 cgcatcacaa taaagaagag tccgagacgc tgcaaagcca gaaagtgacc ggaaacggac
     4261 taccaccaaa accagaagaa cgctggcgct acattaaaga gctggaaagt cgccagccgg
     4321 gtgtgcgtgc gcccacagaa ccttctgccg gtggtgaagt gaaaacgccg gagcaactga
     4381 caccagaaca acgtcagctt cttgaacaaa tgcaggctga tatgcgccag cagccaacgc
     4441 agctggttga ggttccgtgg aatgaacaga cgccggaaca gcgtcagcaa acgctacaac
     4501 gccagcgtca ggcgcagcaa ttagcggagc agcaacgcct ggctcagcag tcgcgtacga
     4561 ctgaacaaag ctggcagcag cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac
     4621 cgcgccagtc taaaccagct tctactcagc agccgtacca ggatctgctg caaacccctg
     4681 cgcacacgac cgcgcagtcg aagccgcagc aagctgcacc ggttgctcgt gccgctgacg
     4741 cgccaaaacc gacggcggag aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt
     4801 tcagaggcgc ggaacaggca gagacggtac gtgctcagct ggcgttcgaa ggatttgact
     4861 cgaaaatcac caccaacaat ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag
     4921 agaacgcaga cagcaccctc aatcggttga agatggcggg tcatacaaac tgcattcggc
     4981 tcgccgccgg gggttgaaac cctcaaaatc ccccccatct ataattgcat tatgcctcgt
     5041 acttttgtac ggggtttgta ctctgtattc gtaaccaagg ggtcagctcg tgacaactat
     5101 agtaagcgta cgccgtaacg gccatgtggt catcgctggt gatggtcagg ccacgttggg
     5161 caataccgta atgaaaggca acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat
     5221 cgcgggcttt gcgggcggta ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa
     5281 actggaaatg catcagggcc atctggttaa agccgccgtt gagctggcaa aagactggcg
     5341 taccgatcgc atgctgcgca aacttgaagc actgctggca gtcgcggatg aaactgcatc
     5401 gcttatcatc accggtaacg gtgacgtggt gcagccagaa aacgatctta ttgctatcgg
     5461 ctccggcggc ccttacgccc aggctgcggc gcgcgcgctg ttagaaaaca ctgaacttag
     5521 cgcccgtgaa attgctgaaa aggcgttgga tattgcaggc gacatttgca tctataccaa
     5581 ccatttccac accatcgaag aattaagcta caaagcgtaa ggatctccca tgtctgaaat
     5641 gaccccacgc gaaatcgtca gcgaactgga taagcacatc atcggccagg acaacgccaa
     5701 gcgttctgtg gcgattgctc tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct
     5761 gcgccatgaa gtgaccccga aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac
     5821 tgaaatcgcc cgtcgtctgg ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac
     5881 caaattcacc gaagtgggct atgtcggtaa ggaagtggat tctattattc gcgatctgac
     5941 cgatgccgcc gtgaaaatgg tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga
     6001 agaactggca gaagaacgta ttctcgacgt gctgatccca cctgctaaaa acaactgggg
     6061 acagaccgaa cagcagcagg aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg
     6121 tgaaggccag cttgatgaca aagaaatcga gatcgatctt gccgcagcac cgatgggcgt
     6181 tgaaattatg gctcctccag gcatggaaga gatgaccagc cagctgcagt ccatgttcca
     6241 gaacctgggc ggccagaagc aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct
     6301 gctgattgaa gaagaagcgg cgaaactggt gaacccggaa gagctgaagc aagacgctat
     6361 cgacgctgtt gagcagcacg ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg
     6421 cggcgagtct tccggtccgg atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct
     6481 ggtagaaggt tgcaccgttt ccaccaaaca cgggatggtc aaaactgacc acattctgtt
     6541 tatcgcttct ggcgcgttcc agattgcgaa accgtctgac ctaatcccgg aactgcaagg
     6601 tcgtctgcca atccgcgttg aactgcaggc actgaccacc agcgacttcg agcgtattct
     6661 gaccgagccg aatgcctcta tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt
     6721 aaatatcgag tttaccgact ccggtattaa acgcatcgcg gaagcggcat ggcaggtgaa
     6781 cgaatctacc gaaaacatcg gtgctcgtcg tttacacact gttctggagc gtttaatgga
     6841 agagatttcc tacgacgcca gcgatttaag cggtcaaaat atcactattg acgcagatta
     6901 tgtgagcaaa catctggatg cgttggtggc agatgaagat ctgagccgtt ttatcctata
     6961 atcgcgttca atcattttca tcattgtttg atggggctga aaggccccat ttttattggc
     7021 gcgtattatg actgaacaac aaattagccg aactcaggcg tggctggaaa gtttacgacc
     7081 taaaaccctc cccctcgcct ttgctgcaat tatcgtcggg acggcgctgg catggtggca
     7141 aggtcatttt gatccgctgg tcgccctgct ggcactgatt accgccgggc tattacagat
     7201 cctttctaac ctcgccaatg attacggcga tgcggtaaaa ggcagcgata gacctgaccg
     7261 cattgggccg ctacgcggca tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc
     7321 gctcattatt accgtcgtgc tcatctgtct ctccgggctg gcactggttg cagtggcgtg
     7381 ccatacgctg gctgattttg tcggtttcct gattcttggc gggttgtcga tcattgccgc
     7441 tatcacctac accgtgggca atcgtcctta tggttatatc ggtctggggg atatttccgt
     7501 actggttttc tttggctggt taagcgtcat ggggagctgg tatttacagg ctcatacatt
     7561 gattccggca ctgatccttc cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa
     7621 cattaataac ctgcgtgata tcaatagcga ccgagaaaac ggcaaaaaca cgctggtggt
     7681 gcgcttaggt gaagtgaacg cgcgtcgtta tcatgcctgc ctgctgatgg gctctctggt
     7741 atgtctggcg ctgtttaatc tcttttcgct gcatagcctg tggggctggc tgttcctgct
     7801 ggcggcaccg ttgctggtga agcaagcccg ttatgtgatg cgggaaatgg acccggtggc
     7861 gatgcgaccg atgctggaac gcactgtcaa gggggcgtta ctgactaacc tgctgtttat
     7921 tttagggata ttcctaagcc agtgggcagc ataactgaca aatatcaatt aacaattgat
     7981 gattttgcca acagcccaca tagcgcgata tactgaaaat tctcgcagca actgaatgtt
     8041 aagcctatga aatacgatac ttccgagctt tgtgacatct atcaagaaga tgttaacgtc
     8101 gtggaaccgc tgttctccaa ctttggcgga cgggcgtcgt ttggcggaca aataatcacg
     8161 gtaaaatgtt tcgaggacaa cgggttgctg tacgatctgc tcgaacagaa tggccgtggt
     8221 cgtgttcttg tcgtcgatgg cggtggttct gttcgtcgcg cactggtcga tgctgaactg
     8281 gcgcgtctgg cagtacaaaa tgaatgggaa ggtctggtta tttacggcgc ggtgcgtcag
     8341 gtagatgacc tggaagagtt ggatatcggc atccaggcga tggcggcaat tccggttggt
     8401 gccgctggcg aaggcattgg cgaaagcgat gtccgcgtca attttggcgg tgtaaccttc
     8461 ttctccggcg accatcttta tgccgacaat accgggatta ttctttcaga agatccgctg
     8521 gatattgaat gataagaaag gcaccgcaag gtgccttttt tctgcgttac ctgttggcct
     8581 acacagtaaa gaaattacgc gaaagatgaa gcgtaatcag acctcttcca tgcgacccag
     8641 cagggcctgc agacgttcct gccagccgtt ctgctgttct ttcagatggt tgttctcacg
     8701 ctccagctct tcgcgctgat gctgggcatt ttgaacttcc tgcgacagtg agttgttttt
     8761 ttctttcagc tcttcgattt ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac
     8821 ttttgcttcc agtttctcaa acacttctaa tgacattgtc atacctctcc tgaattgcaa
     8881 ggcgttgatg gataaaaaat cctcgtcccg attaccggtg acgccttaat aaatacgagc
     8941 gcactttagt tagctccgat tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg
     9001 cagattacgg tttgccacac ttttcatcct tctcctggtg acataatcca catcaatcga
     9061 aaatgttaat aaatttgttg cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat
     9121 ggaataaatg gcgcgataac gctcatttta tgacgaagca cacacatttt aagttcgata
     9181 tttctcgttt ttgctcgtta acgataagtt tacagcatgc ctacaagcat cgtggaggtc
     9241 cgtgactttc acgcatacaa caaacattaa ctcttcagga tccgattatg agtcaaacat
     9301 caaccttgaa aggccagtgc attgctgaat tcctcggtac cgggttgttg atttttttcg
     9361 gtgtgggttg cgttgcagca ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca
     9421 gtgtcatttg gggactgggg gtggcaatgg ctatctacct gaccgcaggg gtttccggcg
     9481 cgcatcttaa tcccgctgtt accattgcat tgtggctgtt tgcctgtttc gacaagcgca
     9541 aagttattcc ttttatcgtt tcacaagttg ccggcgcttt ctgtgctgcg gctttagttt
     9601 acgggcttta ctacaattta tttttcgact tcgagcagac tcatcacatt gttcgcggca
     9661 gcgttgaaag tgttgatctg gcaggcactt tctctactta ccctaatcct catatcaatt
     9721 ttgtgcaggc tttcgcagtt gagatggtga ttaccgctat tctgatgggg ctgatcctgg
     9781 cgttaacgga cgatggcaac ggtgtaccac gcggcccttt ggctcccttg ctgattggtc
     9841 tactgattgc ggtcattggc gcatctatgg gcccattgac gggttttgcc atgaacccag
     9901 cgcgtgactt cggtccgaaa gtctttgcct ggctggcggg ctggggcaat gtcgccttta
     9961 ccggcggcag agacattcct tacttcctgg tgccgctttt tggccctatc gttggcgcga
    10021 ttgtaggtgc atttgcctac cgcaaactga ttggtcgcca tttgccttgc gatatctgtg
    10081 ttgtgaaaga aaaggaaacc acaactcctt cagaacaaaa agcttcgctg taatatgact
    10141 acgggacaat taaacatgac tgaaaaaaaa tatatcgttg cgctcgacca gggcaccacc
    10201 agctcccgcg cggtcgtaat ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa
    10261 tttgagcaaa tctacccaaa accaggttgg gtagaacacg acccaatgga aatctgggcc
    10321 acccaaagct ccacgctggt agaagtgctg gcgaaagccg atatcagttc cgatcaaatt
    10381 gcagctatcg gtattacgaa ccagcgtgaa accactattg tctgggaaaa agaaaccggc
    10441 aagcctatct ataacgccat tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat
    10501 ttaaaacgtg acggtttaga agattatatc cgcagcaata ccggtctggt gattgacccg
    10561 tacttttctg gcaccaaagt gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt
    10621 gcacgtcgta gtgaattgct gtttggtacg gttgatacgt ggcttatctg gaaaatgact
    10681 cagggccgtg tccatgtgac cgattacacc aacgcctctc gtaccatgtt gttcaacatc
    10741 cataccctgg actgggacga caaaatgctg gaagtgctgg atattccgcg cgagatgctg
    10801 ccagaagtgc gtcgttcttc cgaagtatac ggtcagacta acattggcgg caaaggcggc
    10861 acgcgtattc caatctccgg gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg
    10921 tgcgtgaaag aagggatggc gaagaacacc tatggcactg gctgctttat gctgatgaac
    10981 actggcgaga aagcggtgaa atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg
    11041 actggcgaag tgaactatgc gttggaaggt gcggtgttta tggcaggcgc atccattcag
    11101 tggctgcgcg atgagatgaa gttgattaac gacgcctacg attccgaata tttcgccacc
    11161 aaagtgcaaa acaccaatgg tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg
    11221 tactgggacc cgtatgcgcg cggggcgatt ttcggtctga ctcgtggggt gaacgctaac
    11281 cacattatac gcgcgacgct ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg
    11341 atgcaggccg actctggtat ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca
    11401 aacaatttcc tgatgcagtt ccagtccgat attctcggca cccgtgttga gcgcccggaa
    11461 gtgcgcgaag tcaccgcatt gggtgcggcc tatctcgcag gcctggcggt tggcttctgg
    11521 cagaacctcg acgagctgca agagaaagcg gtgattgagc gcgagttccg tccaggcatc
    11581 gaaaccactg agcgtaatta ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg
    11641 gcgtgggaag aacacgacga gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg
    11701 tagtcctgaa acgtgcgggg gcaaccccgc acacatcaat aatcccaccc ttcccctgtg
    11761 ctacacttcg cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg
    11821 aattttcccg cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg
    11881 ataaaaacac cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca
    11941 acattgacgg caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca
    12001 ttggtgaaaa agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg
    12061 aaggcacgcg catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg
    12121 ataaaggctg cttcctcaat gcgccggata tgtatatgga gaagctgatt gtcgggccgg
    12181 gagccaaagg caccattgat ctgaacctgc cgctggcgga taacctgcgc aatgtagcgg
    12241 cggcgctcgg caaaccgttg agcgaactga cggtaacgat tctagctaaa ccacgccacg
    12301 atgccgttat cgctgaaatg cagcaactcg gcgtacgcgt atttgctatt ccggatggcg
    12361 acgttgcggc ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta
    12421 ttggtggcgc gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca
    12481 tgaacggtcg tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca
    12541 ttggcgagca ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc
    12601 gcctgggcga tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag
    12661 gcgatctgct ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga
    12721 tccgcggcaa gtcacgcacc attcgccgca ttcagtccat ccactatctg gatcgcaaag
    12781 acccggaaat gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt
    12841 cgggactgga atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga
    12901 aaaacatggc tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc
    12961 tgtttagtct caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc
    13021 ttggccttga aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg
    13081 ataatcccga tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgat
    13141 tggcggcact gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg
    13201 ttctggatga agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg
    13261 gcccttattt atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc
    13321 tggttcacgc cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg
    13381 aaaaacgcta cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg
    13441 ggtcgctcac cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg
    13501 gcctgccgat gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc
    13561 gcgatacaca acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac
    13621 cgggccatat gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat
    13681 gagcaaatcc gcactcatct tattgatcat cccgcgatat gctggcatca aacggttgat
    13741 aaatgagtga tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag
    13801 cagtgtttcc ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt
    13861 tttcgtcagt ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc
    13921 tgttgcaggg aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa
    13981 cgtcaggtga gcgtcattat gtctggcgag ctccagagct ttattcacca gtaaggcatc
    14041 ttcttcattc ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta
    14101 aagcaactcc atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg
    14161 catgagaaag tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttgtag
    14221 gccacgctgg ccccttcatc tacagaggat gcagcggcgg caagctcttt cagtttggtc
    14281 ataaaagact ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg
    14341 tcgttatcaa tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg
    14401 acatctttca tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa
    14461 tgcgccgtta agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata
    14521 ttgcttaagg tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat
    14581 aacaatcccg gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt
    14641 gtcggcttta tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc
    14701 aacaaacgca ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacaccc
    14761 caccgcccat tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc
    14821 cacaatcatc agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt
    14881 tactgccgct gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag
    14941 taaattttta cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca
    15001 aatccagaaa tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt
    15061 catgtaagtt ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag
    15121 atatcgtttt aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac
    15181 gctgtttttt ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc
    15241 agatacggca acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat
    15301 cagccagttt cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg
    15361 tgccatcgac agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga
    15421 gaacttgtat gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat
    15481 gacctggcta cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata
    15541 catggcagca gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa
    15601 actgcagtcg ctgctaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc
    15661 actgcgttat gttgtcgcgg acaacggcga aaaggggctg accttcgctg ttgaaccgat
    15721 taagctggcg ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt
    15781 gtgccgatga atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc
    15841 tttattcgct tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg
    15901 aaactgaacg gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg
    15961 gcaggtgttg ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg
    16021 aagcgcgaag ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg
    16081 tccggcgcat tcaccggtga aacctctgct gctatgctga aagacatcgg cgcacagtac
    16141 atcatcatcg gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg
    16201 aaaaaattcg cggtgctgaa agagcagggt ctgactccgg ttctgtgcat cggtgaaacc
    16261 gaagctgaaa acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta
    16321 ctgaaaactc agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg
    16381 gcaatcggta ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc
    16441 cgtgaccaca tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc
    16501 ggctctgtaa acgcgtctaa cgctgcagaa ctgtttgctc agccagacat cgacggcgcg
    16561 ctggttggcg gtgcgtctct gaaagctgac gctttcgcag taatcgttaa agctgcagaa
    16621 gcggctaaac aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct
    16681 taactcttcg ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc
    16741 acggttaagc gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg
    16801 ccgtaccatc gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc
    16861 cagcatcata aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta
    16921 ttcatgcccg cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt
    16981 gttatccagt tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt
    17041 ttgcgtacgc ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg
    17101 gccgtacttt ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt
    17161 tgaaggatcg gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag
    17221 atattgcagt gggccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc
    17281 cgcacatggc gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat
    17341 cttacgtaat gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac
    17401 aacggcgata actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct
    17461 gtacctcaaa gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga
    17521 gaaaccaccg ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg
    17581 taccgccgtt agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa
    17641 tggtgaataa cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc
    17701 gcggacggta gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca
    17761 ggtaggcttc cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca
    17821 cggttggctc tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca
    17881 gttcattcgc tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc
    17941 gctcgacaaa ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat
    18001 acagtgcccg aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt
    18061 agccccaggc agccaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga
    18121 tcaccgaaac acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct
    18181 tacgcaccag gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga
    18241 tccactcttt atcaatccgc ccgcgttccg caattgcgtc cacgtcatag gccagagcca
    18301 gcgtgacaac atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac
    18361 cgtgtgactg acgcatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg
    18421 ccttgttgta ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa
    18481 tatcctttgc cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt
    18541 tcatcgcccg actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca
    18601 aacattaaag aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa
    18661 aggcggaaga aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg
    18721 cgcgaacgcc ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt
    18781 aggcctgata agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag
    18841 gaatctgcct ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct
    18901 ttgaacggac gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg
    18961 ttctggatac ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg
    19021 cccatacggg aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg
    19081 cccagcacag ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg
    19141 tcaacatcac acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata
    19201 cccgctttga tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg
    19261 aattcacagc caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc
    19321 acttccacca cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct
    19381 tctacaacgg tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg
    19441 ttgtcgatag tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca
    19501 cccatgtagg aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg
    19561 ttttcgatag ccacggcgcg gatgttctcg tcgcggaatt ccgggaaacg cgcagaaccg
    19621 aggaacgtac cgccacggtt gatcatgtca gacacgctgt aacggtctag ctgtaccata
    19681 cggtcttcat acagacccag atagccgtca taaataccca ttacttccag accttctgtc
    19741 agcgcagaac gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt
    19801 gtcaacacac cgattttctt aatcatgact acctctgaac tttggaatgc aaaatgaaat
    19861 ctgttgccgg aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac
    19921 aatcacttcc tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat
    19981 gcgggtctgg ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt
    20041 acgacggaac aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata
    20101 gcctgctcta cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt
    20161 tccaaatgaa tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg
    20221 ccgctaacac ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc
    20281 aatgcgcgat ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa
    20341 atatagatgc cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac
    20401 aaccccagcg ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata
    20461 tcagcccgca ccgcctggct ttgcgtccgg cgcaccaccc aacgctgaaa cgagacaagg
    20521 ataatcgtac aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctatcatc
    20581 ggtgttggag atatcagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag
    20641 ataaacatac tttgcgccag cgccgcgagg gactctgctt taccgtgacc aaacgagtga
    20701 ttatcgtcgg caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg
    20761 atatccacca gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat
    20821 gcaaaaattt taatcagcaa tagcaacgaa gccatcgccg tcgcagcaat cgccgcccga
    20881 ctgaccagcc gtccataaga ttgattcata aatactcccg ctatcaactg acgctagtat
    20941 aacggaagca aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaacc
    21001 ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac
    21061 tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc
    21121 acgtcacgca actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc
    21181 ggcgttaaca ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt
    21241 tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg
    21301 gtgacaaggc gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg
    21361 gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg
    21421 aacatatggc tctgcgtact gcgctgcgta ctgcgctgcg taagttcttc acccggatgc
    21481 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgaa
    21541 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    21601 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    21661 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    21721 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    21781 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    21841 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    21901 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    21961 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    22021 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    22081 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    22141 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga
    22201 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    22261 aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat
    22321 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    22381 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    22441 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    22501 ctcgattcac gccagatgac cgagcttctg gatagcgaac agcgtcaggg gctgatgatt
    22561 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    22621 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    22681 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    22741 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    22801 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    22861 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    22921 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    22981 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    23041 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    23101 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    23161 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcat tactgcgccg tcgtagcggt
    23221 gaaagcaagg aactggagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    23281 gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    23341 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    23401 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    23461 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    23521 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    23581 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    23641 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    23701 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    23761 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    23821 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    23881 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    23941 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    24001 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    24061 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    24121 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    24181 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    24241 tgcctctccc cagcggaaaa tatcgcccat ataaacatta ctttccgtca ggccgtcggt
    24301 tgagaggttt tcaccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    24361 cgcccagtag agataatgct cacgaggata atgacacagc gctctgtctg gcccaccatg
    24421 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    24481 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    24541 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    24601 accatattta ttttatgtca ggcgtgctgt tttcgcggta agtatccagg caggaactca
    24661 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    24721 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    24781 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    24841 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcata ggtgtcgcta
    24901 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    24961 atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga
    25021 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    25081 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    25141 ggttccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    25201 caaacgaggc aatccctgca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    25261 acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc atgatggaag
    25321 cgtaaagtcc gccattggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    25381 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    25441 cccacgcgac ggcaattttt gttaccacca gcgtaccgga tttacgcaat accgttcccg
    25501 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atggacaccg
    25561 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    25621 tgtggcacag tgcgccaagg aatagcggga cgagcatcat ccccccccgg gattttctca
    25681 atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg ctgatgcgta
    25741 attcctcaat ggagcatcag tgttgatgga aacgaagata gacattcatt taatcaataa
    25801 aagtgatcaa aaccacatca attgaaacgc tgttttattt ttatcggatc attgttttta
    25861 ttttgaaatt gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt ttaagctgat
    25921 atgcggcgta aacgcctcat tgcagcaggc ggcaaatgat tattttttcg ccgcgaaacg
    25981 tgccgctgct tcatcccagt tcaccacgtt ccagaactct ttgatgtagt ccggacggcg
    26041 gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa tcgggaagcc
    26101 ggaagcgcca gaaatagctt cacccatcag cggggaatcc tggttagcag tagaaaccac
    26161 cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc gggaagctgc
    26221 cgctttctca aattctgctt tgaagttatc aacggagccg aagtcacgtt cgatagccgc
    26281 tttcaggtca ccctgtaagg tggtgccttt tttcagacct ttccagaaca ggctgtggtt
    26341 agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg tctgctggca gctggtccag
    26401 tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc
    26461 gttggcgttg ttgacgtagg tctgatggtg tttggtgtgg tggatttcca tggtctgctt
    26521 atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg tatagctcat
    26581 attcatctcc agtattgtcg ggcggccgat tgttaatgcg cgtaagcagt tggttcatta
    26641 tagttaatta aatgatattg aaaatgatta tcaatgccgt acttttcgta agggtatggt
    26701 tttgcaggaa aattcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    26761 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    26821 tcgtcggaga tgtgacgcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    26881 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    26941 caaaaaagta aaaaaatggt catgggaaac catgtggtca gttggtggga ttgtttcgtg
    27001 gattattctg ccgtgggcca tcagcgccct gttactgccg aatttctggg cgtattacag
    27061 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    27121 taatatcaac tacggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc
    27181 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    27241 tgttctgctt aataccgaag gcggacgcat gacgttgctc ggcgttctgg tggcgctgat
    27301 tggtgtaggg attgtgactc gcgccgggca gttgaaagag cgcaaaatgg gcattaaagc
    27361 cgaagagttc aacctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    27421 cggtatgtct tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcactggg
    27481 cgtcgatcca ctgtatgtcg ctctgccaag ctatgtcatc atcatgggcg gcggcgcgat
    27541 catcaacctc ggtttctgct tcattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    27601 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcag cactgggcgg
    27661 gttgatgtgg tatctgcaat tctttttcta tgcctggggc cacgcccgca ttccggcgca
    27721 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    27781 cgggctggtg ctgaaagagt ggaacaatgc aggtcgccgt ccggtaacgg tgttgagcct
    27841 cggttgtgtg gtgattattg tcgccgccaa catcgtcggc atcggcatgg cgaattaatc
    27901 tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac
    27961 caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag
    28021 gcggctatgc tggagaagat attgcgcatg acacactctg acctgccgca gatattgatt
    28081 gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc
    28141 ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctagccgcca gccgggtaat
    28201 cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg
    28261 gcgattcagc aacatcacca actgcccgaa cagcaactca gccatttcgt tagcaaacga
    28321 cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct gcgccatccc
    28381 cacgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc
    28441 ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct gcaaaaccag
    28501 atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga gatcgccacg
    28561 ggtaatgcga taagggcgat cgttgagtac atgcagacca ttaccgcgcc agacaatcac
    28621 cagctcacaa aaatcatgtg tatgttcggc aaaaacatct tgcggataac ggtcagccac
    28681 agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcgc
    28741 caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc gtacaaatac
    28801 gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc ctggcgaata tcacgcggtg
    28861 accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccgcagc
    28921 gataggcgat gtcagtaacg ctggcctcgc tgtgacgtag cagatgtcgg gccttcatca
    28981 gtcgcaggcg gttcaggtaa cgctgaggcg tcagtcccgt ttgctgctta agctgccgat
    29041 gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc atcccaattc acctcatcgg
    29101 caaaatggtc ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt
    29161 tctcctgcaa actgctttta cgcagcaaga gcagtaattg cataaacaag atctcgcgac
    29221 tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc
    29281 gcacctgctg caatacgctg tggttaacgc gccagtgaga cggatactgc ccatccagtt
    29341 cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc gagcgataca
    29401 gcacattggt cagacacaga ttatcggtat gttcatacag atgccgatca tgatcgcgta
    29461 cgaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca tgaatacccg
    29521 tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct
    29581 gcgggagccg gggttctatc gccacggacg cgttaccaga cggaaaaaaa tccacactat
    29641 gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt
    29701 caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat
    29761 acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaatgaa gcaaattgtg
    29821 aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag
    29881 aaggtcacga attcaggcgc tttttagact ggtcgtaatg aaattcagca ggatcacatt
    29941 atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg
    30001 gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat
    30061 ggcctgcata gccagaacgg ttatgtcacc tggaatgtgg atagcctgga aagtgccatt
    30121 cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat tgggcttgat
    30181 acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg cctgcccgtt
    30241 gcttatcgcg atagccgctc caatggccta atggcgcagg cacagcaaca actcggcaaa
    30301 cgcgatattt atcaacgtag cggcatccag tttctgccct tcaatacgct ttatcaactg
    30361 cgtgcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc tctgctgatg
    30421 ccggattact tcagctatcg cctgaccggc aagatgaact gggaatatac caatgccacc
    30481 accacacaac tggtcaatat caatagcgac gactgggacg agtcgctact ggcgtggagc
    30541 ggggccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg
    30601 atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga taccgccagc
    30661 gcggttatcg cctcgccgtt aaacggttca cgcgctgctt atctctcttc tggcacctgg
    30721 tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct ggcagccaac
    30781 ataaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta
    30841 tggctgcttc agcgagtgct tcaggagcgg caaatcaacg atctcccggc gcttatcgcc
    30901 gcgacacagg cacttccggc ctgccgcttc atcatcaatc ccaatgacga tcgctttatt
    30961 aatcctgagg cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc
    31021 ccggaaagtg atgctgaact ggcgcagcta cgcggtgaag atttctcgca actgcatatt
    31081 gtcggcggcg gctgccagaa cacgctgctc aaccagctat gtgccgatgc ctgcggtatt
    31141 cgggtgatcg ccgggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg
    31201 acgctggatg aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat
    31261 ctgaccacct ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagcttcac
    31321 tctacacgac agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc
    31381 tggcgaaaca gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg
    31441 atcgtttacc cgtttcaatg cactgctggc agggcgatga tgtttccggt tttgaaaacc
    31501 cggaaggttc gctgaccggg gggattcagg ccacaggcaa ttatccgggc aaagcgcgta
    31561 atgccagtga gctacgtgcc gatctggaac aggctatgcg gctgattccg gggccgaaac
    31621 ggcttaattt acatgccatc tatctggaat cagatacgcc agtctcgcgc gaccagatca
    31681 aaccagagca cttcaaaaac tggggtgaat gggcgaaagc caatcagctc ggtctggatt
    31741 ttaacccctc ctgcttttcg catccgctaa gcgccgatgg ctttacgctc tcgcatcccg
    31801 acgacagcat tcgccagttc tggattgatc actgcaaagc cagccgtcgc gtttcggcct
    31861 attttggcga gcaactcggc acaccatcgg tgatgaacat ctggatcccg gatggtatga
    31921 aagatatcac cgttgaccgt ctcgccccgc gtcagcgtct gctggcagca ctggatgagg
    31981 tgatcagcaa gaagctaaac cctgcgcacc atatcgacgc cgttgagagc aaattgtttg
    32041 gcattggcgc agagagctac acggttggct ccaatgagtt ttacatgggg tatgccacca
    32101 gccgccagac tgcgctgtgc ctagacgccg ggcacttcca cccgactgaa gtgatttccg
    32161 acaagatttc cgccgccatg ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg
    32221 ttcgctggga ctgcgatcac gtagtgctgc tggatgatga aacccaggca attgccagtg
    32281 agattgtgcg tcacgatctg tttgaccagg tgcatatcgg ccttgacttc ttcgatgcct
    32341 ctatcaaccg cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc
    32401 gtgcgttgct ggaacctacc gctgagctgc gcaagctgga agcggcgggc gattacactg
    32461 cgcgtctggc actgctggaa gagcagaaat cgttgccgtg gcaggcggtc tgggaaatgt
    32521 attgccaacg tcacgatacg ccagcaggta gcgaatggct ggagagcgtg cgggcttatg
    32581 aaaaagaaat tttgagccag cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc
    32641 cggcctacgg gtcggcaaca gttgtaggcc tgataagacg cgacagcgtc gcatcaggca
    32701 ttgattgccg gatgcggcac gagtgcctta tccggcctac aggtcggcaa tagttgtagg
    32761 cctgataaga cgcgacagcg tcgcatcagg cattgattgc cggatgcggc acgagcgcct
    32821 tatccggcct acgggtcgtg catctgacaa caccgaattt acaggaacac agaacatgca
    32881 aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg atgcctggct
    32941 gaaaggctgg gacgaacgca acggcggcaa cctgacgctg cgcctggatg acgccgatat
    33001 cgcgccttat aaagacaacg tccatgcaca accgcgctat atcccgctca gccagcccat
    33061 gcctttactg gcaaatacac cgtttattgt caccggctcc ggcaaattct tccgtaacgt
    33121 ccagctcgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgatg gcgcgggcta
    33181 ccatattctc tgggggttaa ccaacgaagc cgtcccgaca tccgaactcc cggcgcactt
    33241 tctctcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg
    33301 ccacgccacc aacctgatcg ccctcaccta tgtactggaa aacgacaccg cggtctttac
    33361 tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg gcgttggcat
    33421 tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac aagagatgca
    33481 aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga
    33541 tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag tgaaggttta
    33601 ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg
    33661 ctttggcgtt acaccactag ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa
    33721 agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc ggcataaccc
    33781 catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta
    33841 cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagaacggtg
    33901 gaatgcggta gccagtgctg atgtttgcca acgttggtgg aaatatatga ccgatgttat
    33961 gcccgctaac ccggataaca gcccggtgag tagcgagctg c
//
LOCUS       DAJTYD010000027        32898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTYD010000027 DAJTYD010000000
VERSION     DAJTYD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.027"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..209
                     /locus_tag="OO585_001148"
                     /pseudo
     CDS             1..209
                     /locus_tag="OO585_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1877 family protein"
     gene            complement(276..1733)
                     /gene="nuoN"
                     /locus_tag="OO585_001149"
     CDS             complement(276..1733)
                     /gene="nuoN"
                     /locus_tag="OO585_001149"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6628805.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(1740..3269)
                     /gene="nuoM"
                     /locus_tag="OO585_001150"
     CDS             complement(1740..3269)
                     /gene="nuoM"
                     /locus_tag="OO585_001150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6628806.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3433..5274)
                     /gene="nuoL"
                     /locus_tag="OO585_001151"
     CDS             complement(3433..5274)
                     /gene="nuoL"
                     /locus_tag="OO585_001151"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6628807.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5271..5573)
                     /gene="nuoK"
                     /locus_tag="OO585_001152"
     CDS             complement(5271..5573)
                     /gene="nuoK"
                     /locus_tag="OO585_001152"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6628808.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5570..6124)
                     /gene="nuoJ"
                     /locus_tag="OO585_001153"
     CDS             complement(5570..6124)
                     /gene="nuoJ"
                     /locus_tag="OO585_001153"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6628809.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6136..6678)
                     /gene="nuoI"
                     /locus_tag="OO585_001154"
     CDS             complement(6136..6678)
                     /gene="nuoI"
                     /locus_tag="OO585_001154"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6628810.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6693..7670)
                     /gene="nuoH"
                     /locus_tag="OO585_001155"
     CDS             complement(6693..7670)
                     /gene="nuoH"
                     /locus_tag="OO585_001155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6628811.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7667..10393)
                     /gene="nuoG"
                     /locus_tag="OO585_001156"
     CDS             complement(7667..10393)
                     /gene="nuoG"
                     /locus_tag="OO585_001156"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6628812.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10446..11783)
                     /gene="nuoF"
                     /locus_tag="OO585_001157"
     CDS             complement(10446..11783)
                     /gene="nuoF"
                     /locus_tag="OO585_001157"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6628813.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(11780..12280)
                     /gene="nuoE"
                     /locus_tag="OO585_001158"
     CDS             complement(11780..12280)
                     /gene="nuoE"
                     /locus_tag="OO585_001158"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6628814.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12283..14085)
                     /gene="nuoC"
                     /locus_tag="OO585_001159"
     CDS             complement(12283..14085)
                     /gene="nuoC"
                     /locus_tag="OO585_001159"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6628815.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14179..14841)
                     /gene="nuoB"
                     /locus_tag="OO585_001160"
     CDS             complement(14179..14841)
                     /gene="nuoB"
                     /locus_tag="OO585_001160"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6628816.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(14857..15300)
                     /gene="nuoA"
                     /locus_tag="OO585_001161"
     CDS             complement(14857..15300)
                     /gene="nuoA"
                     /locus_tag="OO585_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6628817.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(15931..16869)
                     /gene="lrhA"
                     /locus_tag="OO585_001162"
     CDS             complement(15931..16869)
                     /gene="lrhA"
                     /locus_tag="OO585_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6628818.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            17788..19005
                     /gene="alaA"
                     /locus_tag="OO585_001163"
     CDS             17788..19005
                     /gene="alaA"
                     /locus_tag="OO585_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6628819.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19089..19688
                     /gene="yfbR"
                     /locus_tag="OO585_001164"
     CDS             19089..19688
                     /gene="yfbR"
                     /locus_tag="OO585_001164"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6628820.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(19747..21579)
                     /locus_tag="OO585_001165"
     CDS             complement(19747..21579)
                     /locus_tag="OO585_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6628821.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21666..22316)
                     /gene="hxpA"
                     /locus_tag="OO585_001166"
     CDS             complement(21666..22316)
                     /gene="hxpA"
                     /locus_tag="OO585_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6628822.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPKVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(22327..22821)
                     /locus_tag="OO585_001167"
     CDS             complement(22327..22821)
                     /locus_tag="OO585_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6628823.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(22904..23359)
                     /locus_tag="OO585_001168"
     CDS             complement(22904..23359)
                     /locus_tag="OO585_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6628824.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23697..24899
                     /gene="ackA"
                     /locus_tag="OO585_001169"
     CDS             23697..24899
                     /gene="ackA"
                     /locus_tag="OO585_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6628825.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            24974..27118
                     /gene="pta"
                     /locus_tag="OO585_001170"
     CDS             24974..27118
                     /gene="pta"
                     /locus_tag="OO585_001170"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6628826.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27308..28828
                     /gene="yfcC"
                     /locus_tag="OO585_001171"
     CDS             27308..28828
                     /gene="yfcC"
                     /locus_tag="OO585_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6628827.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVSRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(28861..29403)
                     /gene="yfcD"
                     /locus_tag="OO585_001172"
     CDS             complement(28861..29403)
                     /gene="yfcD"
                     /locus_tag="OO585_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6628828.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29461..30012)
                     /gene="yfcE"
                     /locus_tag="OO585_001173"
     CDS             complement(29461..30012)
                     /gene="yfcE"
                     /locus_tag="OO585_001173"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6628829.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30068..30700)
                     /gene="yfcF"
                     /locus_tag="OO585_001174"
     CDS             complement(30068..30700)
                     /gene="yfcF"
                     /locus_tag="OO585_001174"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6628830.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            30836..31483
                     /gene="yfcG"
                     /locus_tag="OO585_001175"
     CDS             30836..31483
                     /gene="yfcG"
                     /locus_tag="OO585_001175"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6628831.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31540..31902
                     /gene="folX"
                     /locus_tag="OO585_001176"
     CDS             31540..31902
                     /gene="folX"
                     /locus_tag="OO585_001176"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6628832.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            31923..32816
                     /locus_tag="OO585_001177"
     CDS             31923..32816
                     /locus_tag="OO585_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6628833.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
BASE COUNT         8196 a         9020 c         8311 g         7371 t
ORIGIN      
        1 ccgactggaa tcactggatc gaagtaagct gcgtaaacag ttttccatta agcgactatg
       61 aaatggagat ttatccggga gtgacctttt cagaagagtt agaggggcaa ctctttgcct
      121 cgatcatgct tgatatggaa aagctgatta gcgcgtatcg acgtatgctg cgacaaggga
      181 atcatgcgct gacggttatt gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa
      241 catgtcggcg gttttctgac ttacaaacta acagattaca tcagcggcat tgctaaccgc
      301 acaatactaa tcagcgattg tggccataca cccagcacca gtaccaacag tgcagaaatc
      361 agcaccacga taccgcccgc gctgtactgc cagtttgacg gtgcatcgcg acccggttgt
      421 tccggtgcgt gcaggtacag gctcaccgcc acgcgcaggt agtagtagag gccgattgcc
      481 gaaccgacaa ccacggcacc caccagccac cacaagtgtg cctggacacc gaccgccagc
      541 acgtagaact taccgataaa gcccagcgtc atcgggatac cggccagaga cagcatcatc
      601 accgtcatca ctgccgcgag gatcggacga tgccagaaca gaccgcggta agagaacagg
      661 gaatcagcat ccgggccacg atacgggctg gacatcaggc tgaccacgcc gaacgcgccg
      721 aggctgctga acagataacc ggccaggtaa acccctaccg cttccatcga catctcgccg
      781 gtttgcagcg caatcagcgc caccagcaga tagccgaggt gagagataga tgagtaaccg
      841 agcagacgtt tgatgttggt ctggctcaac gccatcaggt taccgaagat gatggaggca
      901 aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt cacccaccgg tgcatagagg
      961 aacagacgca tcaccacacc gaagatagcg attttgctcg ccgtcgccag gaaagtggaa
     1021 accggcgcag gcgcgccttg gtatacgtct ggcgtccaca ggtggaacgg caccagagag
     1081 agtttgaagc cgaggccaac aatcatcatg ccgaaacctg ccagcaacag cggctcgttg
     1141 agcataccgt caccgaggtt tttacccagc gcgacaaacg acaggtcgcc agactgcgca
     1201 tacaccagcg ccataccaaa cagcaggaaa gaagacgctg cggcagaaag gatggtgtat
     1261 ttgatactgg cttccagcga acgtttctgg cggaaagcgt aaccgaccag gccaaacagc
     1321 ggcaaagaga tcagttcaat accgaggaac agagacgcca gatggtttgc attcgccagc
     1381 aggatcccgc ccagcgcggc aattaacacc aacaggtaga actcatcctt gttgtcgtta
     1441 tagccttcaa gccacgggta ggcgaaagta caggtggcga ggctcgccaa caataccagc
     1501 ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg gcgtaacgtc catagcgccc
     1561 gcctggccaa caaaccagag cgaaaccagc gccgcgttaa gcccaataac cgagagcgta
     1621 gcgttgagga aatgattgcg tcgccacgca atggagagca tcacaaccac caccgttaag
     1681 ccgacgatca gcaacggtag cagtgcgatc aggttttgtg gagttattgt catggcgatt
     1741 tacggccttg tagtagtaac ggaattaaca aaccactgct ggatattgcc aatcgcggag
     1801 tgcgaggtat ccagaatcgg ctgcggatag aagcccagca gtaccagcag caccaccagc
     1861 aacaggatca taaacagctc acgcagcgac atccctggca gttcctggct ggcaatctgg
     1921 cttttcgctt taccgaagta agcgcgatgc aacatcgcca gcgaataaac agatgcaaag
     1981 accagcccaa aggtagagat aacggtaatc accgggacaa cctggaagct gccgaacaga
     2041 atcataaatt cgccgacgaa gttaccggtg ccaggcatcc caagcgttgc caccgcaaag
     2101 aacagcgaca atgctggcag ccatttcatc ttgctccaca gaccgcccat catgcgcatg
     2161 tcgcgggtat ggatacgttc ataaagctga ccacaaagaa taaacagacc cgccgccgac
     2221 aaaccgtgcg caatcatctg gattaccgcg ccctggtagg ccaactggct gccggtgtag
     2281 atagcaatca gcacgaagcc catgtgggaa accgaggtgt aggcgatcag acgtttgata
     2341 tcggtctggg cgaaggccat ccacgcaccg tagaagatgc cgataacacc cagccacata
     2401 gcaattggcg cgaactctgc cgacgcgttc gggaacagcg gcagggagaa acgcagcaaa
     2461 ccgtaagcgg cagttttcag caagatcccc gcgagatcaa cggaaccggc ggtcggagcc
     2521 tgggagtgcg catccggcag ccagccatgc agcggaacca ccggcatttt gactgcgaag
     2581 gcgatgaaga agcccagcat taacaggtat tccacaccac tggacattgg cgtattcagc
     2641 agctcttcat agttgaaggt ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc
     2701 aggatggcaa tcaacatcac caggccactc gcctgggtgt aaatgaagaa cttggttgcc
     2761 gccgtgatac gcgttttacc gtcagaggct ttatgccccc acagtgcgat caggaagtac
     2821 atcggcacca gcatcatttc ccagaagaag aagaacagga acatgtcgat ggcaaggaac
     2881 acgccgataa cgccgcccag gatccacatc aggttgaggt ggaagaagcc ctgatatttt
     2941 tcgatctctt tccacgaaca gagtaccgcc agcacaccga gcagaccggt cagcacgacc
     3001 atcagcagcg acagaccgtc aatggcgaga tgaatagaga taccaaaacg cgggatccac
     3061 ggcatgtcga attcagactg ccactgcgga attccggcgg attgcgtcag tgaataaccg
     3121 ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc
     3181 cagcgtggca ccttgacgtc aaagtgttcg gtctgccagc acaggaagcc gccaataaag
     3241 ggaattaata ttagccaggg tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc
     3301 aggaccggat tttcaacgaa ttctcacgac agaaaattct cttcggactg ggggtagtgt
     3361 cggatgcgcc gcgaaggcag cttatccgac ctacgttctt atatgcccag gagcaatccc
     3421 acaatcctta actcaacgca gtaccatcaa cagtgccagc accacgaccg caccgatgct
     3481 catggatgcc acataccagc gcagataacc gttctcgctt aacagcaggc ctttacctgc
     3541 aaagcgggaa agaaccgccg gaatgttcat cattgagttc agcggatcgc gtttcagcaa
     3601 ccaggcaata cccagaaacg gcttgacgaa cactttgtcg tacagccagt caaagcccca
     3661 ggcgttgtac caccaggtgc ccagcagacg gcccggcgca ctgttggcaa tggaggtcac
     3721 cagagtacgt ttaccctgcc acagccaggc tgccagcaga atgccgacca ccgcgaccac
     3781 gccagaggta atttccaggg tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag
     3841 cacgctctgc agcggcggta caatcagtgc gccaacgaag gtggaaagga tcagcagcac
     3901 aatcagcggc aggctgtgag ttaccccttt cacggcgtga gcgtgaattt gttcttttcc
     3961 gtggaagacg atgaaaatca tacggaaggt gtagagcgag gtcataaacg caccgaccag
     4021 acctgccacc atcagattga tatgaccatt cgccatcgca cccgcgagga tctcatcctt
     4081 actgaagaag cccgcagtga ccagcggtag tgccgacagt gctgcgccgc ccaccaggaa
     4141 gcagaaataa accagcggaa tagatttacg cagaccgccc atcttgaaga tgttctgttc
     4201 gtgatggcag gccagaatga cggaaccgga tgccaggaac agcagcgctt taaagaacgc
     4261 gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat
     4321 gtagccaatc tggctcatgg tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag
     4381 cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca atgcccacca gatgcagaat
     4441 ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc aggtagacac ccgcggttac
     4501 catggttgcg gcgtggatca gcgcggagac aggcgtcggg cccgccatcg cgtcggcaag
     4561 ccatgtctgc aacggcaact gcgcagattt accgaccgca ccgcccagca gcatcagcgt
     4621 cgcccacatc agcatgttat tgccgtcagc aaagtgcgct ggtgccagtt ccaccatttc
     4681 gcggaagttc agggtgccca gttcgttgta aagaatgaac agcgcgaaag cgaggaacac
     4741 gtcacccaca cgggtcacga cgaacgcttt cattgccgct gcgccattct tcggatcggt
     4801 gtaatagaac ccgatcagca gataggagca caggcccacg ccttcccagc cgaggtacat
     4861 cagcagcagg ttatcggcga gaaccagaac taccatgctg gcgatgaaca ggttggtgta
     4921 ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac caggaggcgt acatgtgaat
     4981 aaggaaaccc acaccagtga ccaccgagag catggtcagc gacaggccgt ccagcaccag
     5041 gttaaaaccg atgttaaagt cgcctaccga catccacgtc cacagcggct ggctgtatgc
     5101 ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc
     5161 cacagagcca acgccgacga tcgccgaaac gttttcagac cagcgcccac gggagaacgc
     5221 cagcaggacg aagccaatca atggcaaaat aatggttaag gcaagcatgt tcatccgcgc
     5281 atctcactta ctgaatcgat gttcaggttc tggcgacgac ggtgaagttg cagcagcagc
     5341 gcaaggccga tactcgcttc tgccgccgcg aggctgatgg cgagaatgta catcacctga
     5401 ccgtcggtct gaccccagta gcttccggcg accacaaagg ccagcgcgga ggcgttaatc
     5461 atgatttcca gaccaatcag cataaacagc agattgcgac ggataaccag accggttaag
     5521 ccaagaacga ataagattgc cgagaggatc agtccatgtt gtaaggggat catgcgtgct
     5581 cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct cagcacttca cccgcacgct
     5641 cttcacgacc gacgtggaag gccacaacca gacccgcgag cagcagcata gaagccagtt
     5701 ccaccgccag tacgtaaggc ccgaacagcg taatacccac tgctttagca ctgattggcg
     5761 taccgtcgat accctgatca ttaacaccaa ggatggcgta aacaatcacc accagcatga
     5821 tggccgacaa aattgccgga ccaatccaca cctgcggttt cagccactgg cgttcctgtt
     5881 cgatttctga accgcccagg ttgagcatca tcaccacgaa cacgaacagc accataatgg
     5941 cacccgcgta gacgataatt tccagcgcac cggcgaagta agcgcccagt gagaagaaca
     6001 ccccggaaat cgccagcagc gaaataatca ggtacagcag tgcgtgtacc ggattggtat
     6061 gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc acagatataa aaagcgaact
     6121 ccattgcccc tctccttacg gtaacaggct cttgacgtcg ataggcttgg cttcgttctc
     6181 tgcttcgccc ttatctttgc cgtcgattgc catacctgcc atccggtaga agttatattc
     6241 cgggtatttg cccggaccgg agatcagcag atcctctttt tcgtaaacca gatcctggcg
     6301 cttgtattcc cccatttcga aatccggggt taactgaatc gccgtggtcg gacaggcttc
     6361 ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg cggaaaaatt ccgggtacca
     6421 gcgaccgtct ttggtttctg ctttttgcag cgagatacag ccgaccgggc aggctaccgc
     6481 gcagaggtta caggctacgc aacgctcttc gccgtccggg tcgcgggtca gaacgatacg
     6541 accacgataa cggggcggca gatagaccgg ctcttccggg tacattcgcg tttcgcgttt
     6601 ggcgaacgcg tgcaggccga tcatccagat actacgaacc tgggtgccga aacctactaa
     6661 caattctttt aaggtcatgg tcttattgcc ccttattgcg cctgccagag aatgacagcc
     6721 gccgttacca gcaagttgat cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt
     6781 acctggtcat aacgcggacg cggtaacgac gcacgaatca aaatgaacat catcataaag
     6841 aacgcggttt tcaacgcgaa ccagatgaat ggcggtaaca acgggccttg ccagccaccg
     6901 aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc cgatgtactc acccacgaag
     6961 aacagaccga acttcatacc ggaatattca atgtggtaac catccgccag ttcctgctcg
     7021 gcttccggct ggtcaaacgg gtgacggtga cataccgcca cgcccgcgat ggcaaaggta
     7081 ataaaaccaa agaattgcgg gataacgttc cacacatgcg cctggctgtt gacgatgtcg
     7141 gtcatgttga atgaaccggc ctgcgccacc acgcccatca gggaaagccc gaggaacact
     7201 tcgtagctca gggtctgcgc agaagcacgc atcgcaccca gcaacgagta tttgttgtta
     7261 cttgaccagc ccgcaaacag caccgcgtaa accgccagac ctgccatcat caggaagaac
     7321 aaaatcccga tgttcaggtc ggcaaccacc caacccggac tgactggcac aatcgcaaag
     7381 gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg taaagatgac gcgatccgag
     7441 aatttcggga tccagtcttc tttaaagaac attttgatca tgtccgcaac cagctggagc
     7501 gaaccgcccc agccaacacg gttaggtcca taacggttct ggaacagacc cagcaggcga
     7561 cgttcgccaa agctcatgaa tgccccacag gtgacaacca ccagcaggat caccaccgct
     7621 ttgaggatgg tcagcaggat ctcaatcagt tccggtgata tccaactcat tgttgtgcct
     7681 ccttgagatc ctcaagatgc gcgccagcca gcaccggagc aatgccggac atacccatcg
     7741 gcaagcccac ctgccctgcc gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt
     7801 tgccatcgta actaaaggag acgcgtgtac ctgcgttcac acccaacttc gcggcatccg
     7861 ctgggttgag tttgatgtac ggctgcggca tacggctctg gaagaccgga gcacgctgtg
     7921 acaattcatc gctgccaaac aggtggtaat acggcgcaat acgccatttc ccgtcctgcg
     7981 gctggaagcg tgccggtacg ctggtgaagt aatccagacc attctcgctg gtttcaaaca
     8041 gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc
     8101 acgcctgcgg ggagttccag cccggtgccc aggcaaacgg cacttgcgaa cggtgcgcgg
     8161 tcggctggtt gttaccttcc atcgagaagg tgaacatggt gtcaatatcc tgcggctgac
     8221 gcggctcatg aacgctgata ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt
     8281 cacgggccag tttctgccca cgaatacgga atgtcgcatc cggcgcagca tctttgatac
     8341 ctgccagttc cgggattttc gccacaacag cgtcaatcac atggtcgagc tgcgtccagt
     8401 ccacttcacg gctcagcagg gtgctgtgca gcgagtgcag ccagcgccag ctttccagca
     8461 tgacagtttt gctgtcgtaa taagccggat cgtaaacctg gaagaaacgt tgggcgcgac
     8521 cttcattatt aattaccgta ccgtcgcttt cagcaaagct ggcggcggaa agtaccagat
     8581 gggcgttttc catgatcgct gtacgctgat ggtcaaccac catcaccagc ggtgctttag
     8641 ccagcgcagc attcacgcgg gtagcagaag cgtgacgatg cagatcgttt tccaacacca
     8701 ccaccgcgtc ggcgcgtccg gtttccagtt cggttaacgc ttcttcaagc gaaccgccac
     8761 ccataatgcc cagccccatg ctgttgacgg aacgggcaat catggtgata ccgacgtcag
     8821 cgccgcgacc tttcagggct ttcgcgacgt tagccgccgc ctgaatcacc tctaagctac
     8881 cggcgttcgt cccggagata atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga
     8941 cgtcgatttt gctttgcagc tcaggttcga taccgtcaac cgctggtgca gagttatcca
     9001 gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc
     9061 acgccgcgat atcatccaga cgggtgtcat caacgttggt aacaaacagc ggatgcttcg
     9121 cacgttgacc gatgttaagg attgccgcaa tctgccagtc agccactttc tgtgctgccg
     9181 ccatttcgcg cgctttacct ttcaccgcct gacgcactgc cagcgcgacg cgcgcgccgg
     9241 tctgggtaac gtcttcaccc agcaccagta ccgcatcgta agattcgatt tcgcgcagag
     9301 ccggagtata aatgccgcct tcacgcagca ctttcagcgc cagttgcaga cgttcctgct
     9361 caccgtgagc gataccggtg tagaagtttt cttcgcccac cagttcacgc agtgcaaagt
     9421 tgctttccac gctggcacgc ggagaaccaa taccgatcac tttcttcgac tgacgcagaa
     9481 tatctgccgc gccctgcatt gcctgttcgg cgttgagggt aatgaaatca tcgccacgac
     9541 gctgtactgg ctgacgcgga cgatccttca ggttgacgta accgtaaccg aaacgaccac
     9601 ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg gttttcgata cgacgcagtt
     9661 cgccgtaacg ttcaccgggg ctgatgttac agccgatgga acattgctgg cagatgctcg
     9721 gcgcaaactg catatcccat ttacggttgt aacgctcgga gtgcgttttg tcggtaaata
     9781 cgccggtcgg gcaaatttcg accaggttac cggaaaattc gctttccagc gtgccgtctt
     9841 ccgggcgacc gaagtagacg ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag
     9901 cgtaatcttt gtagtaacgc acacagcggt agcaggcgat gcagcggttc atttcgtgag
     9961 atatgaatgg ccccaaatcc tgattacggt gggtacgttt ggtgaaacgg tagcgacgga
    10021 agctgtgtcc ggtcatcacg gtcatatcct gaagatggca gttaccgcct tcttcacata
    10081 ccggacagtc gtgcgggtgg ttggtcatta accactcgac cacactttca cggaactgtt
    10141 tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga agccggtgtc atacaggaca
    10201 tcaccaggcg accacgcgtg tcttccgcgt tttggtattg cttcaccgca cactggcggc
    10261 aagcaccgac gcttcccagc gccggatgcc agcaaaagta aggaatatca aggcccagag
    10321 acagacaagc ttccagcagg ttgtccgctc cgttgacctc gtattctttg ccgtctacat
    10381 gaattgtagc cattagcatg cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa
    10441 ttcggttacc agcgctcttt cagcaggttc ggctgaatcc cattaatcaa atgggtattg
    10501 ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt
    10561 aacggctcca ctgcaccagg tgcgtgggca cagaaagttt tacccgggcc taagaatcga
    10621 cacagttgct caagtgtttc aatatcgccc ggctggcctt cgccacgctc cagcgcacgc
    10681 agaattttca cgctccacgg cagaccgtcg cggcacggcg tacaccagcc gcaggactca
    10741 cgggcgaaaa actcttccag gttacgcacc agcgacacca tgttgatctc atggtcaacc
    10801 gccatcgcca gcgccgtacc cagacggctg cccgctttac cgatactttc gaattccatc
    10861 ggcagatcaa ggtgcgcttc ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct
    10921 ttaaatttca gaccatcacg cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg
    10981 gtgccgaacg gcagttccca cagtcccgga tttttcaccc gaccggagaa gcccatcagc
    11041 ttggtgccag catctttact tttcgagatg ttctgatacc actccacgcc gttagcgagg
    11101 atcgccggaa cgttacacag ggtttcgacg ttgttgacac aggtcggttt gccccatacg
    11161 ccggaggttg ccgggaaggg gggtttcgag cgtgggttag cacgacgccc ttctagggag
    11221 ttgattaacg ctgtttcttc cccgcagatg tagcgccctg ccccggtatg gacgaacagt
    11281 tcgaagtcga aacctgttcc cataatgttt ttgccaagca agcccgcttc agtggcttcg
    11341 gcaatggcgc ggcgcagatt tactgccgct tcgatatatt cgccacgcag gaagatgtag
    11401 ccacggtaag ctttcagcgc aaacgcggag atgagcatac cttccaccag caggtgcggc
    11461 agttgctcca tcaacaggcg gtctttatag gtgcccggct ccatttcatc ggcgttacac
    11521 agcaggtaac ggatgttcat ggattcgtct ttcggcatca ggctccactt caggccagta
    11581 gaaaagcccg ctccgccgcg ccctttcaga ccagcgtctt ttacctgatt aacgatttcg
    11641 tccggagaca gcccggtcag cgccttacgc gccccttcgt aaccgttttt gctgcggtat
    11701 tcgtccagcc acactggctg tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg
    11761 ggagtacgga taatgttttt catttatacc gctccagcag ttcagggatc gcttccgggg
    11821 tcagatgcgc gtgagtgtcc tcatcgatca tcatgtttgg ccctttatca cagttcccca
    11881 ggcagcaagt tggcagcagc gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt
    11941 tcagcttttt ctcgagcgcc gcctgaatac cctgataccc gttgatatga cagaccacgc
    12001 tgtcgcaata acggatcaca tggcgaccaa ccggctggcg gaagatctga ctgtagaacg
    12061 ttgccacacc ttcgacgtcg cttgccggaa tacccagcac atcggcgatc gcgtggatcg
    12121 caccatccgg cacccagcca cgctgcttct gaacgatttt cagcgcttca atggacgccg
    12181 cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc ttcacgctct gccgcactca
    12241 gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat aattagcggt ccacatctga
    12301 cataacaaaa tcgatactgc ccagataaac aatcaggtca gacaccaggc tgccgcggat
    12361 cgccgccgga atttgctgca aatgcgcata actcggcgtg cggatacgag tgcgataact
    12421 catggtgcta ccgtcgctgg tcaggtagta actgttgatc cccttggtcg cctcaatcat
    12481 ctggaaagat tcattggcag gcatcaccgg cccccacgac acttgcagga agtgggtgat
    12541 cagggtttcg atatgttgca gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc
    12601 cgctttgaac gggccttccg gcatgttgtt gaggcactgc tcaagaatgc gcagactctg
    12661 gcgcagctct tccactttca gcattacgcg ggtgtagcag tcagaaacac caccaccaac
    12721 cgggatttca aagtcgaagt tttcatagcc agaataagga cgcgccttac gcacgtcgaa
    12781 gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc cactccagcg cctctttcgc
    12841 gccataggcg gcaacgccct gggaacgacc tttcagaatg gtattttgca gcgccgcttt
    12901 ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac tcacgcagca ggcgatccca
    12961 gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac cacgccgggt gcatacggaa
    13021 acccgtgatc gcttccacca gatcgtaaat tttctgacga tcggtaaagg cgaagaacac
    13081 gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg tacagcaggt ggctgttgat
    13141 acggaacagt tcggagagca taacgcgaat gacgttaacg cgatccggca cggtgatccc
    13201 ggccagtttc tctaccgcca gcacgtaagg catttcgtta acgcagccgc cgaggtattc
    13261 aatacggtcg gtatacggaa tgtagctgtg ccaggactgg cgctcgccca ttttctcagc
    13321 accacggtgg tggtagccga tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg
    13381 taaaacgata cggaaagccc cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat
    13441 gaagtcctcg ttttcggtgc cgcgcttcat cccccactct tccggtttga aggtcagggc
    13501 ttccatctcc agatcctgtt tggctttggt cagctcaaac ggcgagaatt cggtagcgcg
    13561 cgccgggtaa tctttacgca gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg
    13621 caggttcggg tgaccgtcga aagtaatgcc aaacagatcc caggtttcac gctcatacca
    13681 gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc
    13741 caccttcagc atgatgtcgc ggttacgatc gatagaaatc agatggtaga aaacggaaaa
    13801 atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc
    13861 aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa tcgccaactt ccagtaattg
    13921 ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc
    13981 cggcccaaaa cggttgcgca gttcgccaat taccggatca tcaagatgat cgcgggtctg
    14041 ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc accattgcaa atggtccgtg
    14101 gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc
    14161 tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg
    14221 ttcaccgcgc ttgcgttcgc gctctgattg catattggcg cgataaacgc cctgatcgcc
    14281 aaccacccag gagagcggac gacgttcttt gccgatagat tcctgcaaca gcatcagtgc
    14341 ctgcatgtac gcttcaggac gcggcgggca gcccgggata tacacatcaa ccgggatgaa
    14401 tttatcgacg ccctgcacaa cggaataaat atcgtacata ccaccagagt tggcacaggc
    14461 acccattgag ataacccatt ttggttccag catctggtca tacagacgct gaataaccgg
    14521 tgccattttg gtaaagcagg ttcctgcaac caccatcagg tcagcctgac gcggcgaagc
    14581 acgcaatact tctgcgccaa aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat
    14641 ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa attgagtttt tacgacccca
    14701 gttaaccatg tcattgagct tgcccataaa cacgtttttg ttaacttctt gctccagagg
    14761 gtcggttacg atctcctgct tttgcagggg gtaacggtcg ttctcaccgt tgggatctat
    14821 gcgggtgagc gtataatcca tcttaatgcc tcgcggttag cgttgacgat tagcgatact
    14881 gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc
    14941 aatacgcacc agataaacca gacctgccag taacacaaaa ataaaaattg cagcttccac
    15001 aaagcctacc cagccgcttt cgcggataga ggttgaccat gcgaacagat acagcgcttc
    15061 aacgtcgaag ataacgaaga acatggccac cagataaaac ttggcggaca ggcgtaagcg
    15121 ggcggagccg accgagtcga taccggattc aaacggcacg tttttcgacc tcgcgcgtgc
    15181 gcgaccgcct aaaaaccaac cacctaccag cattaggcaa cacaggccaa tggcaacgat
    15241 aagaaagata gcgaatgccc agtgatgagc gatgacttca gtggatgttg acatactcat
    15301 tgcttactca tcaaaagtag cgccagattc actgctcttc acggcagatg gacgccacat
    15361 cgattcatgg ggaggaataa aaaaaacctt acaatcactg tagaaattct tttatacagc
    15421 taattgatgt ggtcttttac tcctttctat aaccttttgt caactttaac aaaagtttct
    15481 tcacattagt ttacataata tcaacaccat tagcatttaa tgccctttca ccccagctcc
    15541 ttgacgactc caggataatt agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa
    15601 aatacgggtc tattttgaca ggaatttgtg tcgattcctc cccccaaaag agagtatttt
    15661 cttgatctgt gacacgcttt tgtcattcca taacaaaaac gcagcaacaa atttacgtat
    15721 tttttaacat cattgtagca ggtgattttt ttcaggcgat tatttgtgcg ttcgggacgt
    15781 gaatctctgg tgattaaaaa atgaacagtt ttgtacgttc tgcactatgc gaggaaggat
    15841 ttttactaaa aaaaagccgc tggggtttaa aacaccccca gcggctcgtt ttttacacta
    15901 ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa tcaacaatga
    15961 atcatcccct tccggagcag acattgggct gtattgccac ggattatggt agctctccat
    16021 cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata
    16081 ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca ggtccgggct
    16141 catcatctca accggccttg ccgtcacgcc aagaccggct ttcactgccg cacgaacggc
    16201 cggaagcgtc gaggcgacat aagccagtcg ccatggaata tctgctttat taagcgttgc
    16261 cagcaccata tcgcgaaacg gacttggatc atccagcaat acaagaggga tcggctcgcc
    16321 tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa
    16381 tgccttaaac gctgaaggac gatgggtggt taccatcaaa tccacttcct gggattccag
    16441 catttctgcc atataggcat tacgtttaac gcggacatcc agcgctaatt tcggataaac
    16501 cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc
    16561 gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa
    16621 acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt
    16681 gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg
    16741 acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc
    16801 ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc
    16861 acttatcata tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac
    16921 acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg
    16981 aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc acattgttcc gttataatgc
    17041 ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt actcactctt
    17101 ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc
    17161 gtgcagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg cggcatcaag
    17221 agcagggatc cgtcatttat gcgagcattt taccccaaaa accttttatt tataagggcc
    17281 attgcgaatc atctgatgca aagttatgtt atgttaggca aagtaatctt ctattattaa
    17341 taagcacatc aaaacctttt tgaatattaa ataataatta attagcatca tcctcattca
    17401 ttaattccgc ttaacaatag tttcacacct tgccccatta cgacacctcg gaaatcatca
    17461 cgctgttaaa ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta
    17521 aatgaggcat tttcacacaa ttatcttaca gataaaaaaa ccagacttac aattaagaat
    17581 cagaacaatt cactatataa cattgcatgt aaagcatata cacctcatta ttttgtcatc
    17641 attaagttat taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc
    17701 ttcaaaacac cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg
    17761 cgataacagc aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag
    17821 aatgtctgtt atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa
    17881 ggtaacaagg tactgaaact gaacatcggc aacccagccc cgttcggttt tgacgcgcca
    17941 gatgaaatcc tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc
    18001 aaaggtcttt actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt
    18061 gatgttaccg tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca
    18121 atgcaggcat tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccacta
    18181 tggaccgcgg cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc
    18241 tctgactggt tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg
    18301 atcgttatta tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg
    18361 gagattgtgg agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac
    18421 aaaattcttt acgacgacgc tgagcatcac tcaattgcgc cgctggcacc tgacctgctg
    18481 accattacct ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg
    18541 atggtgttga acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg
    18601 gcttcaatgc gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt
    18661 ggttatcaga gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac
    18721 cgcgcgtggg aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg
    18781 ctgtatatgt tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg
    18841 gtgctggatt tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg
    18901 ccgtggccgg atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct
    18961 ttgagcaagt tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc
    19021 cggagattgc atccggcagc gttatcccgc cacaatgacc tgatgatgtc atcatacgta
    19081 aggtcactat gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc
    19141 gctggccgct aatgcgcaac gtgctgacgg aaaatgtgtc cgaacacagt ttgcaggtag
    19201 cgatggtcgc ccatgcgctg gcagctatca aaaatcgaaa atttggcggt aatgtcaacg
    19261 ccgaacgtat cgctttactg gcgatgtacc acgatgcctc agaagtgctc accggcgatc
    19321 tccctactcc ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa
    19381 aaatcgctca gcaaaaactg gtcgatatgg ttccggaaga gctgcgggat atctttgcgc
    19441 cgttaattga cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg
    19501 cactgtgtgc atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc
    19561 tggcaaaaac gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact
    19621 tcatggaagt atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac
    19681 cgctgtaagc agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg
    19741 ctcttgtcaa aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat
    19801 ggtgaacggt acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac
    19861 cagtgtgtta acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac
    19921 gaccatcgcg aatggataag gcgacacccc catcgttttg gcagcagcca gcgcaatcgg
    19981 agccatcaac accgccgtcg cggtattaga gataaatagc ccaataaccg ccgacaagac
    20041 aaacaaacac cccagcatca tatgtggccc gtaaccaccg ccaatgtcca tcagcccttt
    20101 caccgccagc gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac
    20161 gatcaaaata atgctcggcc agtgaatgga tttataggcg ctttcagcat ctatacagcg
    20221 gaatttcccc atcagcaggc aggcgatgat agcggcaaca ggattaggaa tttcatctgt
    20281 cagcattaac gccaccatta gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc
    20341 gggcgatgct tcactcacct cttccggtaa gttcagcgct acgaagtcgc ggccctgttt
    20401 ggccagcata ccgatcagtt tccagttacc cacaaccagg atgatatcgc ccagcagcag
    20461 aggctcatcc gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt
    20521 cagtccgtag cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc
    20581 aggaattaat gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc
    20641 gcgcagtacc atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc
    20701 cgcagacata tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt
    20761 cacgataacg cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg
    20821 ctcacgtaat ttgagatcat ccagccgttg accaatcatt ggcgatccgg ggcgaatagc
    20881 cagacgtcgc gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg
    20941 cgtccagcct tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa
    21001 catatacaaa atacccagca ccagcacgac caggccaaca ggtgttacgc taaagaaact
    21061 gaagccgtga tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc
    21121 caccagcgtc atcatgccgc taatcagccc ggcaaaactt aacggcatca tcagacgtga
    21181 cggcgaagtt tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac
    21241 gccggttgaa ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat
    21301 tttgatttca ctgttgcccg ccactttgac cagccatgtt cccattacgg tggcaacacc
    21361 ggtacggacc aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc
    21421 agaaaagccg gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat
    21481 aacaaacaaa gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac
    21541 ggccagtaat gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag
    21601 ggggatgact tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag
    21661 gtgtttcact gaataataac atcgccattt agctgtttgg tcacagtaat ttgctccaga
    21721 ctgtggagga ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca
    21781 atgacatggc aacccgccgc caggccagaa agcacgccag cgggagcatc ttccgccacc
    21841 acacactcct gcggcgcaag ccccagcagc tgcgcaccta acagatacgc atcaggttct
    21901 ggttttccgc gcttcactcg ctcagcggtt acaaacacct ttggtgcggg aagcccagct
    21961 attttatggc gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata
    22021 cctgctttat tcaaatgatt gagtaaggcg attgcccccg gaagcgcggt aataccttcg
    22081 gtttccgtgg cctcgatgtg ctccagacac gtaaactcgg cggcaatatc agcctcggat
    22141 ttgcccgcca taaaatggcg caaagaggtg atcgcctgtt taccgtgaat gaaagccagc
    22201 acctcttccg gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca
    22261 ggcagggaat ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg
    22321 gcctcctcag gcgttgataa tttgattaat ttcgttggcg ctcaaatggt actgacgcag
    22381 gcaggcatgc cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt
    22441 aaaaccatga gtcccagcgt caaaatgggt atagcgccct tccacattaa ccataaagcg
    22501 gacataaccg aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg
    22561 ttcatcgatg gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat
    22621 ctccataatg tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc
    22681 caattcacgc atctgtaatc cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg
    22741 ttcagcattt gccggatcga gcatagtcat catcttgtac tgattagaca aaataagacg
    22801 ttgcgcgttg gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaag
    22861 aaggctgagt atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga
    22921 aagttttatc cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag
    22981 gcttgccttc aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat
    23041 aaaaccagtt gaggatagca gggggtaatg gcgttacaga acgcttgccc agccaccata
    23101 atccctgcat gggtaaactt aaggcgaaca gcgcagtggc aactgccggc ccaagctgac
    23161 cgcccagggc aatctgccag cagagagtaa atacggcgat cggcggcata aaacggatcg
    23221 cataacgcgt catcttgata acgcgatttt cgacaaagac cggggcaagg cgtttttcca
    23281 gcggccacgt ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa ttaacagaac
    23341 gattatccgg cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg
    23401 caaattcaca actcagcggg acaacattca aaacattttg tcttccatac ccactatcag
    23461 gtatccttta gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata
    23521 tacattatgc cattggctga aaattacgca aaatggcata gactcaagat atttcttcca
    23581 tcatgcaaaa aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc
    23641 tacgctctat ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt
    23701 cgagtaagtt agtactggtt ctgaactgcg gtagttcttc actgaaattt gccatcatcg
    23761 atgcagtaaa tggtgaagag tacctttctg gtttagccga atgtttccac ctgcccgaag
    23821 cacgtatcaa atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg
    23881 ctcacagcga agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt
    23941 ctgcgcagct gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag tataccagct
    24001 ccgtagtgat cgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc
    24061 tacacaaccc ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga
    24121 aagacaaaaa cgttgctgta tttgacaccg cgttccacca gactatgccg gaagagtctt
    24181 acctctacgc cctgccttac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc
    24241 acggcaccag ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag
    24301 aagaactgaa catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca
    24361 acggtaaatg cgttgacacc tctatgggcc tgaccccgct ggaaggtctg gtcatgggta
    24421 cccgttctgg tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga
    24481 gcgttgacgc aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag
    24541 tgaccagcga ctgccgctat gttgaagaca actacgcgac gaaagaagac gcgaagcgcg
    24601 caatggacgt ttactgccac cgcctggcga aatacatcgg tgcctacact gcgctgatgg
    24661 atggtcgtct ggacgctgtt gtattcactg gtggtatcgg tgaaaatgcc gcgatggttc
    24721 gtgaactgtc tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaacc
    24781 tggctgcacg tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg
    24841 ttatcccaac caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat
    24901 ttcacaccgc cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga
    24961 aagaggataa accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct
    25021 gaccagcgtc agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt
    25081 tttcaaacct atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat
    25141 cgtgcgtgcg aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga
    25201 aggtctgctt tccagcaatc agaaagatgt gctgatggaa gagatcgtcg caaactacca
    25261 cgctaacacc aaagacgctg aagtcgttct ggttgaaggt ctggtcccga cacgtaagca
    25321 ccagtttgcc cagtctctga actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt
    25381 cgttatgtct cagggcactg acaccccgga acagctgaaa gagcgtatcg aactgacccg
    25441 caacagcttc ggcggtgcca aaaacaccaa catcaccggc gttatcgtta acaaactgaa
    25501 cgcaccggtt gatgaacagg gtcgtactcg cccggatctg tccgagattt tcgacgactc
    25561 ttccaaagct aaagtaaaca atgttgatcc ggcgaagctg caagaatcca gcccgctgcc
    25621 ggttctcggc gctgtgccgt ggagctttga cctgatcgcg actcgtgcga tcgatatggc
    25681 tcgccacctg aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc
    25741 cgtcactttc tgcgcacgca gcattccgca catgctggag cacttccgtg ccggttctct
    25801 gctggtgact tccgcagacc gtcctgacgt gctggtcgcc gcttgcctgg cagccatgaa
    25861 cggcgtagaa atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc
    25921 taaactgtgc gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac
    25981 ctggcagacc tctctgagcc tgcagagctt caaccaggaa gttccggttg acgatcacga
    26041 acgtatcgag aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgaatc
    26101 tctgactgcc acttctgagc gcagccgtcg tctgtctccg cctgcgttcc gttatcagct
    26161 gactgaactt gcgcgcaaag cgggcaaacg tatcgtactg ccggaaggtg acgaaccgcg
    26221 taccgttaaa gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg
    26281 taatccggca gagatcaacc gtgttgcagc gtctcagggt gtagaactgg gtgcagggat
    26341 tgaaatcgtt gatccagaag tggttcgcga aagctatgtt ggtcgtctgg tcgaactgcg
    26401 taagaacaaa ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct
    26461 cggtacgctg atgctggaac aggatgaagt tgatggtctg gtttccggtg ctgttcacac
    26521 taccgcaaac accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct
    26581 ggtatcttcc gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc
    26641 gatcaacccg gatccgaccg ctgaacagct ggcagaaatc gcgattcagt ccgctgattc
    26701 cgctgcggcc ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca ccggtacttc
    26761 tggtgcaggt agcgacgtag aaaaggttcg cgaagcaact cgtctggcgc aggaaaaacg
    26821 tcctgacctg atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc
    26881 gaaatccaaa gcgccgaact ctccggttgc aggtcgcgct accgtgttta tcttcccgga
    26941 tctgaacacc ggtaacacca cctacaaagc ggtacagcgt tctgccgacc tgatctccat
    27001 cgggccgatg ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt
    27061 tgacgatatc gtctacacca tcgcgctgac tgcgattcag tctgcacagc agcagtaatc
    27121 tcgtcatcat ccgcagcttt gcgctgcgga tatctgaacc ggaaataatc actatttccg
    27181 gttttttatt ctcttaatct gcattaatcc tttctgatta tcttgcttaa ctgcgctgca
    27241 tcaatgaatt gcgccatttc actttgcata cttaccactt tgttttgtgc aagggaatat
    27301 ttgcgctatg tccgcaatca ctgaatccaa accaacaaga agatgggcaa tgcccgatac
    27361 gttggtgatt atcttttttg ttgctatttt aaccagcctt gccacctggg tagttccggt
    27421 ggggatgttt gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa
    27481 agtcgtagat ccacactcat ttcgcattct gactaacgaa gcaggcgaac ctgagtatca
    27541 ccgcgtacag ctgttcacga cgggcgatga acgcccgggc ctgatgaact tcccgtttga
    27601 aggattaacc tcaggatcga aatacgggac agctgttggc atcatcatgt ttatgctggt
    27661 gattggcggc gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc
    27721 gcttattcgc catacccgcg ggaatgaaat tctctttatt cctgcgctgt ttattctgtt
    27781 ttcacttggc ggcgcggtat ttggtatggg agaagaggcc gtcgcctttg ccattatcat
    27841 cgcaccgcta atggtccggc tgggctatga cagtattacc accgtcctgg tgacctatat
    27901 tgccacgcaa atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg tcgttgctca
    27961 ggggattgcc ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat
    28021 cgccactatg attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc
    28081 tcttctgtca cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg cggatgttga
    28141 acaacgtccg tttacctttg gtgactggct ggtattgatt gtcctgaccg ccgtaatggt
    28201 ctgggtgatt tggggcgtga tcgttaatgc ctggtttatt ccagaaattg ccagccagtt
    28261 cttcaccatg ggtctggtga ttggcatcat cggcgttgtt tcccgcctta acggcatgac
    28321 ggttaatacc atggcttcat cctttaccga aggggcgcga atgatgatcg cccctgccct
    28381 gctggtgggt ttcgccaaag ggattttgct gctggtcggt aatggtgaag cgggtgatgc
    28441 cagcgtgtta aataccatcc tcaacagcat tgccaatgcc attagcggtc tggacaacgc
    28501 ggtcgcggcc tggtttatgt tgctcttcca ggcggtattt aatttcttcg tgacgtccgg
    28561 ttctggtcag gcggcgttaa ccatgccgtt actggcaccg cttggcgatc tggtcggtgt
    28621 taaccgtcag gttaccgtgc tggcattcca gtttggtgat ggcttcagtc acatcattta
    28681 cccaacctca gcttcgttaa tggcaactct cggtgtgtgc agggtggact tccgtaactg
    28741 gctgaaggtg ggcgcgaccc tacttggact gctgtttatt atgtccagcg tcgtagtgat
    28801 cggcgctcag ttgatgggct accactaaaa atgttaagag ccgcaattgc ggctcttttt
    28861 tcattctgcc gtttcagtct ctacggcttc atttttggca ttgcgcttca tccacaacgc
    28921 cagcgctttc agcgagtctg gagtgaactc atcgcagcgt gcggtgattt cttccggcgt
    28981 cagccagcaa acttcactga cttcatcttc ctgtagggcg aagggaccgt gagagacgca
    29041 gctgaacaac gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc
    29101 ggcaaagggg acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg cggattccag
    29161 cagttgctca tcggcctgaa ccacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa
    29221 gtcttttgtc tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat
    29281 gtaagttgca cgatgacgca gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat
    29341 gacttcgttc tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc
    29401 cattatcagg aaaccttctt tttctggcgc tcttacggcg cattttgagt tgtgggtaaa
    29461 ttacggatta atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag
    29521 tacgtcatta tccagcatgc cataactcgc cggattaccg cctttcggaa tactcaccga
    29581 gccggggttg aagtggaaaa tctccccccg ctgttctgcc actggcagat gggtatgacc
    29641 gtacaccagc acatcgttct ggtttaaagc aggtagattt tccgggccaa aaagatgacc
    29701 atgcgtcaaa aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg
    29761 gaaatgcagc agcatttgat ccacttcgct gtcgcagttg ccgcgcacag cgataacctt
    29821 atgtgccact tcattaagcc gttcagcgac tttggctggc gcgtaaccct ccggtaaagc
    29881 attacgcggg ccgtgattca acacgtcgcc aaggatcacc agccactggg caccgctttg
    29941 ggcaaacaac tccagaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa
    30001 catcagtttc atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc
    30061 gttgctatca gccagattgc ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct
    30121 gccactggaa tgtcgcataa tccaccaggc gttctggcac ctcatcgcca tgtagtacca
    30181 ggcggttaat cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg
    30241 gctgacccag tgctaacaaa tgttctgcca tcgcgacact ggcttttccc tcggccgtta
    30301 gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg cggatgggca
    30361 tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg cgcacgattt tctaaatcaa
    30421 gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg
    30481 cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac
    30541 cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc gaggtcgatg gtcttgatat
    30601 gaaacgacag gcctttttcc tgcaacgcca cccaagcgga taacacataa ggggagaaaa
    30661 agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg
    30721 aggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgtaa
    30781 gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag ctgcaatgat
    30841 cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc tcgaagaagc
    30901 agagcttgat tatcgcttga ttaaggtaga cctggggaaa ggcggtcagt ttcgtccgga
    30961 atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt ctcctgccga
    31021 tggcggcgaa ccgctaagcc tctttgaatc tggtgccatt ttgttgtatc tggctgagaa
    31081 aacggggctc tttttgagtc atgaaacgcg tgaacgcgcc gccacattac agtggttatt
    31141 ctggcaggta ggcgaactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc
    31201 cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctttacca
    31261 tgtactaaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact acagcattgc
    31321 ggatattgcc tgctggccgt gggttaatgc ctggactcgc cagcgaattg acctcgcaat
    31381 gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc
    31441 actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt
    31501 gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt
    31561 attcgtataa agaaccttcg tttacgtacg tttatcggaa ttaaggaaga agaaattaac
    31621 aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact
    31681 agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat
    31741 gtagagaata accgtttctc tttgttggaa aaattaactc aggatgtgct cgatatcgca
    31801 cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc
    31861 tacgccgatt cggtatccat gaccttaagc tggcagcgtt aaccgccata tcgggaggct
    31921 gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg attccgcgtt
    31981 tgctggagct gggccatcaa atcacggtag tgacgcgtaa cccacagaaa gccagttccg
    32041 ttctcggccc tcgggtgacg ctatggcaag ggctagccga tcaaagcaac ctcaacggcg
    32101 ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg actcacgagc
    32161 aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta
    32221 atgccagcga cacgccgccg tcggtactca tttccggctc ggcaacgggc tattatggcg
    32281 acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt actcataaac
    32341 tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg cgagtgtgcc
    32401 tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa atgctgccac
    32461 cgtttcgtct tggcctgggc gggccgattg actctggtcg gcagtatctg gcctggattc
    32521 atattgatga tatggtcaac ggtattctct ggctgctgga taacgagctg cgcgggccat
    32581 ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg ctcggtcacg
    32641 cgctgcatcg cccggccatt ttgcgcgtcc ccgcgaccgc cattcgactg ttaatgggcg
    32701 aatcttcagt actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg
    32761 gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg
    32821 gtttacagca aacatccgcc agttaactcc cggtgttaca ggattagtgg ctttgcgcga
    32881 taagatcgtc tggtgaaa
//
LOCUS       DAJTYD010000028        32630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTYD010000028 DAJTYD010000000
VERSION     DAJTYD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.028"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..258
                     /locus_tag="OO585_001178"
     CDS             <1..258
                     /locus_tag="OO585_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6628834.1"
                     /translation="GLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSV
                     VMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            268..1642
                     /gene="gspE"
                     /locus_tag="OO585_001179"
                     /pseudo
     CDS             268..1642
                     /gene="gspE"
                     /locus_tag="OO585_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1632..2834
                     /gene="hofC"
                     /locus_tag="OO585_001180"
     CDS             1632..2834
                     /gene="hofC"
                     /locus_tag="OO585_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6628835.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2869..3912)
                     /gene="guaC"
                     /locus_tag="OO585_001181"
     CDS             complement(2869..3912)
                     /gene="guaC"
                     /locus_tag="OO585_001181"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6628836.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4068..4112)
                     /locus_tag="OO585_001182"
     CDS             complement(4068..4112)
                     /locus_tag="OO585_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628837.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4137..4757
                     /gene="coaE"
                     /locus_tag="OO585_001183"
     CDS             4137..4757
                     /gene="coaE"
                     /locus_tag="OO585_001183"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6628838.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4757..5500
                     /gene="zapD"
                     /locus_tag="OO585_001184"
     CDS             4757..5500
                     /gene="zapD"
                     /locus_tag="OO585_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6628839.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5510..5707
                     /gene="yacG"
                     /locus_tag="OO585_001185"
     CDS             5510..5707
                     /gene="yacG"
                     /locus_tag="OO585_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6628840.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5814..6203)
                     /gene="mutT"
                     /locus_tag="OO585_001186"
     CDS             complement(5814..6203)
                     /gene="mutT"
                     /locus_tag="OO585_001186"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6628841.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6263..8968)
                     /gene="secA"
                     /locus_tag="OO585_001187"
     CDS             complement(6263..8968)
                     /gene="secA"
                     /locus_tag="OO585_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6628842.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9030..9542)
                     /gene="secM"
                     /locus_tag="OO585_001188"
     CDS             complement(9030..9542)
                     /gene="secM"
                     /locus_tag="OO585_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6628843.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9773..10690)
                     /gene="lpxC"
                     /locus_tag="OO585_001189"
     CDS             complement(9773..10690)
                     /gene="lpxC"
                     /locus_tag="OO585_001189"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6628844.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10791..11942)
                     /gene="ftsZ"
                     /locus_tag="OO585_001190"
     CDS             complement(10791..11942)
                     /gene="ftsZ"
                     /locus_tag="OO585_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6628845.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12003..13265)
                     /gene="ftsA"
                     /locus_tag="OO585_001191"
     CDS             complement(12003..13265)
                     /gene="ftsA"
                     /locus_tag="OO585_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6628846.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13262..14092)
                     /gene="ftsQ"
                     /locus_tag="OO585_001192"
     CDS             complement(13262..14092)
                     /gene="ftsQ"
                     /locus_tag="OO585_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6628847.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14094..15014)
                     /gene="ddlB"
                     /locus_tag="OO585_001193"
     CDS             complement(14094..15014)
                     /gene="ddlB"
                     /locus_tag="OO585_001193"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6628848.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15007..16482)
                     /gene="murC"
                     /locus_tag="OO585_001194"
     CDS             complement(15007..16482)
                     /gene="murC"
                     /locus_tag="OO585_001194"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6628849.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16536..17603)
                     /gene="murG"
                     /locus_tag="OO585_001195"
     CDS             complement(16536..17603)
                     /gene="murG"
                     /locus_tag="OO585_001195"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6628850.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17600..18844)
                     /gene="ftsW"
                     /locus_tag="OO585_001196"
     CDS             complement(17600..18844)
                     /gene="ftsW"
                     /locus_tag="OO585_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6628851.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18844..20160)
                     /gene="murD"
                     /locus_tag="OO585_001197"
     CDS             complement(18844..20160)
                     /gene="murD"
                     /locus_tag="OO585_001197"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6628852.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20163..21245)
                     /gene="mraY"
                     /locus_tag="OO585_001198"
     CDS             complement(20163..21245)
                     /gene="mraY"
                     /locus_tag="OO585_001198"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6628853.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21239..22597)
                     /gene="murF"
                     /locus_tag="OO585_001199"
     CDS             complement(21239..22597)
                     /gene="murF"
                     /locus_tag="OO585_001199"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6628854.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22594..24081)
                     /gene="murE"
                     /locus_tag="OO585_001200"
     CDS             complement(22594..24081)
                     /gene="murE"
                     /locus_tag="OO585_001200"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6628855.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24068..25834)
                     /gene="ftsI"
                     /locus_tag="OO585_001201"
     CDS             complement(24068..25834)
                     /gene="ftsI"
                     /locus_tag="OO585_001201"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6628856.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25850..26215)
                     /gene="ftsL"
                     /locus_tag="OO585_001202"
     CDS             complement(25850..26215)
                     /gene="ftsL"
                     /locus_tag="OO585_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6628857.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26212..27153)
                     /gene="rsmH"
                     /locus_tag="OO585_001203"
     CDS             complement(26212..27153)
                     /gene="rsmH"
                     /locus_tag="OO585_001203"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6628858.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27155..27613)
                     /gene="mraZ"
                     /locus_tag="OO585_001204"
     CDS             complement(27155..27613)
                     /gene="mraZ"
                     /locus_tag="OO585_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6628859.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28215..29219)
                     /gene="cra"
                     /locus_tag="OO585_001205"
     CDS             complement(28215..29219)
                     /gene="cra"
                     /locus_tag="OO585_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6628860.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29301..29387)
                     /locus_tag="OO585_001206"
     CDS             complement(29301..29387)
                     /locus_tag="OO585_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6628861.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29399..29890)
                     /gene="ilvN"
                     /locus_tag="OO585_001207"
     CDS             complement(29399..29890)
                     /gene="ilvN"
                     /locus_tag="OO585_001207"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6628862.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29893..31617)
                     /gene="ilvI"
                     /locus_tag="OO585_001208"
     CDS             complement(29893..31617)
                     /gene="ilvI"
                     /locus_tag="OO585_001208"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6628863.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPISISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31736..31780
                     /locus_tag="OO585_001209"
     CDS             31736..31780
                     /locus_tag="OO585_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628864.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31935..>32630)
                     /gene="leuO"
                     /locus_tag="OO585_001210"
     CDS             complement(31935..>32630)
                     /gene="leuO"
                     /locus_tag="OO585_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6628865.1"
                     /translation="QLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCSPLDSILT
                     SQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHRPEFTSVPLFKDE
                     MVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQGMAM
                     MSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKQNSRTCYLSWHEAAGRDKGH
                     QWMEEQLVSICKR"
BASE COUNT         7820 a         9172 c         7950 g         7688 t
ORIGIN      
        1 ggattagata cctgcgacgg tggcagcaat ggcattccct cgcctaccac cacccgctat
       61 gtttcagcca tgagtgtggc aaaaggcgtg gtgtcgctga ccgggcaaga aagtctcaat
      121 ggactaagcg tcgtcatgac gccaggttgg gataacgcaa acggcgtcac cggctgggcg
      181 cgcaactgca atattcaaag tgacagcgca ttgcagcaag cctgcgaaga tgtcttccgc
      241 tttgatgacg ccaactaagg agcggcaatg aatattccac agctcaccgc cctgtgtctg
      301 cgttatcagg gagtcttgct ggatgccagc gaagaggtgg ttcatgttgc ggtagtcgat
      361 gcaccttcgc atgagttgct ggacgcattg catttcgcta ccaccaaacg tattgagatc
      421 acctgctgga cgcgccaaca aatggaaggc cacgccagtc gcacacaaca gacattgccc
      481 gtagctgttc aggagaagca tcagtcgaaa gcagagttgc tgactcgaac gttacaatct
      541 gcgctggaac aacgtgcgtc tgatattcat atcgaaccag cggacaatgc ctaccgcatc
      601 cgcttgcgta tcgacggcgt attgcatcct ttaccggatg tttcaccgga tgccggagtc
      661 gcattaaccg ccagattaaa agtgctggga aacctggata ttgcggaaca tcgcctgccg
      721 caggacgggc aattcactgt cgaactggca ggaaacgccg tctcatttcg tattgcgacc
      781 ttaccatgtc ggggtggtga aaaggtggta ttaaggttgt tacagcaggt gaaccaggca
      841 ctggatgtta acacgctggg aatgcagccg ttacaactgg cggactttgc tcatgccttg
      901 caacaaccac agggactggt gctggtaact ggccctacag gcagcggcaa aacggtcacg
      961 ctttatagtg ccctgcaaaa gctgaatacc gctgacatta atatttgtag cgtcgaagat
     1021 ccggttgaga tccccatagc cggactaaac cagacgcaaa tccatccgcg tgccgggctc
     1081 acctttcagg gcgttttgcg tgcgttattg cgccaggatc ctgacgtcat catgatccga
     1141 tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc ggtcacctgg tgttgtctac
     1201 cctacacact aattccacct gcgaaacgct ggtacgttta cagcaaatgg gagtcgcccg
     1261 ctggatgctc tcatcagcgc ttacgctggt aatagcccag cgtctggtac gtaaattttg
     1321 cccacattgt cgccagcagc aaggggagcc catccatatt ccagacaatg tatggccgtc
     1381 gccgctgccc cactggcagg cacccggttg tgtacattgc taccacggtt tttatggtcg
     1441 taccgccttg tttgaagtcc tgcccattac accagccata cgccagctta tttccgctaa
     1501 taccgacgtt gaatcgctgg aaacgcacgc acgacaggcg ggtatgcgta cgctttttga
     1561 aaacggctgc ctagccgtgg agcaaggctt aaccaccttt gaagagttaa tccgcgtact
     1621 ggggatgccg catggcgagt aagcaactct ggcgctggca tggcataacc ggcgacggca
     1681 atgcgcaaga tgggatgcta tgggcagaga gccgtgcttt gctgctcatg gcactacagc
     1741 aacagatggt taccccactt agcctgaagc gaatcgccat caattctgcg cagtggcgag
     1801 gagataaaag cgcggaagtc attcatcaac tggcgacgct actcaaagcc gggttaacgc
     1861 tttctgaagg gctggcactg ctggcggaac agcatcccag taagcaatgg caagcgttgc
     1921 tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt ttccaatgcc ttattaccct
     1981 ggtcagaggt atttccgcca ctctatcagg cgatgatccg cacgggtgaa ctgaccggta
     2041 agctggatga atgctgcttt gaactggcgc gtcagcaaaa agcccagcgt cagttgaccg
     2101 acaaagtgaa atcagcgtta cgttatccca tcatcatttt agcgatggca atcatggtgg
     2161 ttgtggcaat gctgcatttt gttctgccgg agtttgccgc tatctataag accttcaaca
     2221 ccccactacc ggcactaacg caggggatca tgacgctggc agactttagt ggcgaatgga
     2281 gctggctgct ggtgttgttc ggctttctgc tggcgatagc caataagttg ctgatgcgcc
     2341 gaccgacctg gcttatagtg cggcagaaat tgctgttacg catcccgatt atgggttcac
     2401 tgatgcgggg acaaaaactc acgcagatct ttacgattct ggcgctgaca caaagtgcag
     2461 gcattacttt tttgcagggc gtagagagcg tcagagaaac aatgcgctgc ccgtactggg
     2521 tgcaacttct gacacaaatc cagcacgata tcagtaacgg tcatcccatc tggctggcgc
     2581 taaaaaatac cggggagttt agcccgctct gtttgcaatt agtgagaaca ggagaggcat
     2641 ccggctcgct ggacctcatg ttagacaacc tcgcccatca tcatcgggat aacacaatgg
     2701 cgctggcgga taacctcgca gccttactgg aaccggcgtt gctgatcata acgggaggaa
     2761 ttatcggtac gctggtggtg gcgatgtatc tgccaatttt ccatttaggc gatgcgatga
     2821 gtgggatggg ataacgctgg cgtgttgctc cacaccagcg ttggaagatt acaggttgtt
     2881 gaagatgcgg ttttcttgtt cctgcacacg gataaatgtg gtgcgcttgg tcagttcttt
     2941 caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg ccgcccaaaa tatcacgcgc
     3001 ggtattttca accgggcctc gcagcggcag cttaacggtt ttaccttctg ctgcgcgata
     3061 ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg gagctcatgc cgtagaacag
     3121 cataaatttc tcgccgttct cctcaacgat gcgaccgccg ctctcttcgt ggcccgccag
     3181 catgccgcca agcatgacga aatcggcacc gccgccaaag gcttttgcga catcgcccgg
     3241 cgtggtgcag ccaccgtcgc tgacgatcat tccgcccaga ccgtgcgccg catcggcaca
     3301 ttcgattacc gcagaaagct gcggataacc gacgcctgtt ttgacgcgag ttgtacaaac
     3361 agaacctggg ccaatgccaa ctttaacgat atcagcaccg gagaggataa gctcctcaca
     3421 catttcacca gtcactacgt tgccagcaca aatggttttg gtcggccacg cttcacgcgc
     3481 tttcgcaacg aactgcacga agtgttcgga ataaccattc gccacatcaa tacaaacaaa
     3541 gtttaatgcc gggttcaggt cgagaatctg tttagttttt tcgaaatccg catcagacgt
     3601 accggtagaa accatcacat gtttcagcac atcagcggaa gaattgttga taaacgcttg
     3661 ccactcttcg acagaatagt gtttatgcac agcagtcaaa atatcaaaag aagccagcgc
     3721 agaggccata gaaaatgtac ctacggtgtc catatttgcg gcgataatcg gcacgccgga
     3781 ccagctctga tctgaatgtt tgaaggtgaa ttgacgttcc agttcaacat cggaacggct
     3841 tttaagagtg gagcgtttag ggcggatgag aacgtcttta aaacctaact tcagatcttc
     3901 ttcaatacgc atgtgcggat tcctggggtt aatggctata agggtaagag cgcaactcca
     3961 gtgacgctat catacgcact aatcaatgcg gcgcaagact gcgaaattgt ctttttttac
     4021 gctacaatcc cataaattta cctggtaaat agtgagcgaa cgtttcatca atttcgcttt
     4081 cgtgcactca tttttaacgt tttgataatc atgatgaatt cccgggaata taaattatga
     4141 ggtatatagt tgccttaacg ggaggcattg gcagtggcaa gagtaccgtt gccaatgcgt
     4201 ttgctgatct cggaattaac gtcattgatg ccgatattat tgcgcgtcag gtggttgaac
     4261 caggtgcacc tgcgctacat gccattgctg atcactttgg cgctaacatg attgctgctg
     4321 atggaacatt gcagcgccgg gccttgcgcg agcggatctt cgccaacccg gaagagaaaa
     4381 actggcttaa cgccctgctg catccgctga ttcagcaaga gacgcaacac cagatccagc
     4441 aagctacttc cccctatgta ctgtgggttg tgccattgct ggtagaaaac tcactgtata
     4501 aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga aacgcaactt aagcgcacca
     4561 tgcagcgcga tgatgtaact cgcgagcatg tcgaacaaat ccttgctgct caggcaacgc
     4621 gcgaagcccg ccttgccgtg gcagatgacg tcattgataa taacggcgca ccggatgcta
     4681 tcgcatcgga tgttgcccgc ctgcacgcac actatttgca gcttgcgtcg cagtttgtct
     4741 cacaggaaaa accgtaatgc agacccaggt cctttttgaa catccactaa atgaaaaaat
     4801 gcgtacatgg ctgcgcattg agtttttgat tcagcaactc accgttaatt tacccatcgt
     4861 tgaccacgct ggcgcgctgc atttcttccg taatgtcagt gaattactgg atgttttcga
     4921 gcgcggcgaa gtccgcactg agctgttgaa agaacttgac cggcagcaac gtaaactcca
     4981 gacttggatt ggcgtgcctg gcgtggacca gagccgtatt gaagcattaa ttcagcagtt
     5041 aaaagcggcg gggagcgtat taatttccgc gccgcgtatc gggcaatttc tgcgtgaaga
     5101 tcgtttgatt gctctggtgc gtcagcgact gagcatccca ggcggctgtt gcagctttga
     5161 tttacctaca ttgcacattt ggctgcatct accccaggcg cagcgcgaca gccaggtaga
     5221 aacctggatt gccagcctga acccgctcac ccaggcactt accatggtgc tggatttaat
     5281 tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat ggtttttatc aggataacgg
     5341 tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat tcacagcttt atccgcaaat
     5401 ttccggacat aagagccgtt ttgccattcg ttttatgccg ctggacagtg aaaacggaca
     5461 ggtaccggaa cgtctagatt tcgaactggc ctgttgctaa ggagtgaaaa tgtcagaaac
     5521 tattacggtg aattgcccaa cctgcgggaa aacggtggta tggggtgaaa tcagcccgtt
     5581 tcggccattt tgctccaaac gttgtcagct gatcgacctc ggagaatggg ctgctgaaga
     5641 aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat gactggagcg aagagccaaa
     5701 gcagtgacat tggtctgatg ccgcacatag gcctgataag acgcggacag cgtcgcatca
     5761 ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta tccgacctac agactacaga
     5821 cgcttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc attaagaccg
     5881 accagcgaca tccactcacc gggttgccct tctttacccc acggcacccc ttcccaactt
     5941 tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatatgc cagtttttca
     6001 aatagcgaaa aatgttgtgg ggtaatcccg acctcttcct gaagttcacg caccaccgcc
     6061 tgttccggcg tttcgcccat ttcaatttta ccgccgggaa actccagttt attcgccatg
     6121 tgcgcatctg ctgcgcgacg cgttataaag atttcattgt tctcgttgcg aataatacct
     6181 accgcaattt gcagcttttt cattgtttta aacctataaa aaaggcgcag aatcctgcgc
     6241 cttttgcttc aacagttagc ttttattgca ggcggccatg acactgcttg tattttttac
     6301 cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ctcaccggtt tgcgccgcca
     6361 gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt tgcgctaaac
     6421 gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc atacgtacct
     6481 gaactttgct cagcgtactg ataacttcat atttcaacga ctccagcatc gctgcaaaca
     6541 tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag ccacgcaggt
     6601 ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg gagtcaagcg
     6661 tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc acttcttctt
     6721 tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc tcttcatgca
     6781 gttctggttc tttatccagc cactcggcaa ttggtaaatc gaggtcgaaa tcgttcttca
     6841 gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc ggaatgtagg
     6901 catcaatggt ggctttgaac acatcttcac gaatgctgtt gatggtttcg ctcacatcgc
     6961 tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg ttagccacgt
     7021 catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact ttacgctggg
     7081 cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc ttcataccca
     7141 gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc gcatcttcca
     7201 tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga ccgcgcaact
     7261 ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg ccacctgctt
     7321 ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt tgctctgcgg
     7381 tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca atatctgtac
     7441 cacgacccgc catattggtc gcgatagtca ccgcagccgg ataacctgct tgagcaacaa
     7501 tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt gtgcttaata ccggctttgg
     7561 tcagttcgtt tgacaccagc tccgattttt cgatggagat agtacccacc agcaccggct
     7621 ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt tccgcttcag
     7681 tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc ggaacaacga
     7741 cggtatccag cttgtagatg gagctaaatt cgaaagcttc ggtatcagca gtaccggtca
     7801 tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa gccagcgttt
     7861 ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc agaccatcgg
     7921 accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata acttcaccat
     7981 ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg agcgcgcagc gccgccgtta
     8041 cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca tccatgatgc
     8101 cctctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg ttcacctggc
     8161 gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag tcttcttttt
     8221 cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc gagctgtctt
     8281 ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg gagtccactt
     8341 cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg aacgccatat
     8401 tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg tcagctgcgt
     8461 aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt gataccgaca gtcaggccaa
     8521 ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag tcgttgacgg
     8581 taactacgtg cacgccttta ccggttagtg cgttcaggta agcaggcagc gttgcggtca
     8641 gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga accataccgc
     8701 cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc tcacgtacca
     8761 cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga cgtgcacgaa
     8821 actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc ggttccatgg
     8881 cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta cgactaccga
     8941 aaactttagt taacaatttg attagcataa taaaatctca aacgccccgc gttgcggagt
     9001 taataaaatg aagtaaaggt ttattgttgt taggtgaggc gttgagggcc agcacggatg
     9061 ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt aaaatgcgca
     9121 taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcagcag cgcgctgagc
     9181 gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc aacgggcagt
     9241 gtttgcggtg ccattgcgaa agaaagatga cggattaccg tgcgaatggc atattgatgc
     9301 cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt tcgcttccag caaggccaat
     9361 tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt tgcgggcacg
     9421 tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcgac catccctaat
     9481 aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag tattccactc
     9541 acgttattgc catcccgatc gaattcaaac gtatttagcg cacaaatctt atcattaaag
     9601 cctgaagtcc acagcatgaa tgaagataag ttgtgcgtaa aagtgcttaa aaaaacagcg
     9661 ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa aacgactggt tcacctggcc
     9721 ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt cgttatgcca
     9781 gtacagctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg aaggtcacat
     9841 attcccaagc ttcctgtttc gccaggacag cctgcagcag tttgttattc agtgcatgac
     9901 cggatttata agcgataaat gcaccaataa tattgtgacc acacatgaac aagtcaccga
     9961 tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg tcttcgttca
    10021 gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg cacaaaccac
    10081 gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg ctgatctggc
    10141 gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa tcaatagccg
    10201 gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta aattcagccc
    10261 acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttt ttggcgcagt
    10321 tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg ctgccgtcca
    10381 tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc aggcccgcga
    10441 gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg accagacacg
    10501 tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc ggtggattca
    10561 agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac gtcagagtga
    10621 ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta agtgtccttt
    10681 gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac tatacattcg
    10741 gcgggccagt ttagcacaaa gagcctcgaa accaaaattc cagtcaattc ttaatcagct
    10801 tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt ttgcggcgca
    10861 ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc catcccatgc
    10921 tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag agtgatttca
    10981 ggacgtttgt ccatgccgat acctgtcgca acaacggtta cgcgcagctc gtcattcata
    11041 tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa tgcacggatg
    11101 gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgccgt gatgttaacc
    11161 agcacgccgc gcgcgccaga caggtcgata tcttccagca gcggagaaga gatagccatt
    11221 tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat cattgcgtag
    11281 cccatctcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa acccggacga
    11341 gtaatcagtt cagcgatacc ttgcacagcg cctttcagta catcgttcgc tgcgccaaac
    11401 gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt cgggatagtg
    11461 atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa tgccatacgc
    11521 ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat acccaaatct
    11581 tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc catacccgca
    11641 gcaataaaga ccatgtctgc accttccagc gccgcacgca atgcatcgcg atcctcatca
    11701 gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt gataccgcta
    11761 ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc ggtatttacc
    11821 gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc attaccgccg
    11881 ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat tggttcaaac
    11941 atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg gcctcataaa
    12001 aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc aactgatgct
    12061 gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc atagtgaagc
    12121 aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc ggtaatgttc
    12181 agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc tgcaagacct
    12241 tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg tttaacccct
    12301 tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt gagcagctcg
    12361 gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg tggcggacga
    12421 ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc cagcgcacaa
    12481 ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc gtaagcgata
    12541 tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa tgccccaccg
    12601 gtataaacgg cgatatccat tgtaccacct ccgatatcga cgacgcagac acccagttca
    12661 cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat cagttggtca
    12721 actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc gttgtgacat
    12781 gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg attcttgatc
    12841 ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg atgttcatcg
    12901 cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg cgtcacttct
    12961 tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt accagaaagc
    13021 gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc aatggcgcgt
    13081 tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat accacgtgac
    13141 gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc ccctactaaa
    13201 gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc cgtcgccttg
    13261 atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct ggcggcaagg
    13321 gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta atccgtttgc
    13381 catcggtttg cgccagctgc tgtaaaaccg gataaagttc tacaaagcga gccaaacgtt
    13441 tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc aactgccagg
    13501 aacgccgcgc ggtcatcgcc gtttccttca gagtaaatct gtcctttgcc agcatctgcc
    13561 ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca tacagcattg
    13621 gaagcacctg cttgctggtg cgatctggcg gcacgctgaa ggtgtttcct tccgcgtcta
    13681 ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga atcttcaatt
    13741 catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt tgttctattt
    13801 gcgtctggat gatgttgaca tcctgggtca taaaggtacc cggctcaccc aatgccagga
    13861 tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac accagctttg
    13921 agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg ctcaccaaca
    13981 ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca ttattgcggc
    14041 gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac atattagtcc
    14101 gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg acgtgccgcc
    14161 atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag cagataaaac
    14221 tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc taacgtcgtc
    14281 catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcatccag acctgcgggg
    14341 cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa ggttccggac
    14401 ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt gaactccggc
    14461 ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa tcttaatgca
    14521 tcttgtagag cattttctgc tactactttt gacattccca cactggaacc ttcgcggctc
    14581 ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt atcgctcagg
    14641 cctttttcaa actctgcgcg ggttaacgct acccacggcg cgaccggtaa accggcacct
    14701 tgccacagca gtttgctgcg tagtttatcc attgaaagcg cagatgccat cactccgctg
    14761 ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtaccatc ttcaccgccg
    14821 cgaccgtgta gcgcgataaa cactttctga aaacccatcg acttcagttg cgtcacgtcg
    14881 acttctttcg ggtcgacagg atacgcatca ataccacctt cacgcagccc ggctaacacc
    14941 gctgcgccag aattcagaga aacttcccgc tcagcggaag tcccgcccaa caggaccgcg
    15001 attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga tttcagctaa
    15061 agaacgggca atttttccga tattaccagc cccctgaacg agaatcaggt cgttaccggt
    15121 taataccggt gccagcatct cggctacctg cgcagggtcc ggcaccagaa tgggatcaat
    15181 tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg gaattggcgc
    15241 ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca gcacattggc
    15301 gaaatcatca tacaagtcgc gcgtacgggt aaaacggtgc ggctgaaaca gcattaccag
    15361 gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg gcgtcaactt ccgtcgggtg
    15421 gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg gctccagcgg
    15481 gaattcaccg aggaaatcaa aacggcgacc agtccccagg aagctctcca gcgcacgcaa
    15541 aatagcctcg tcgtcaattc cctcttccgt agcaaccgca accgcagctg cggcgttcag
    15601 cgcgttatga cggcctggcg cattcagggt gacgcgcatc ggctctttgt cctggcgcag
    15661 cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca cgtcggcatc
    15721 ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc ggtaacaatt cgcggatcac
    15781 cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca gaaaattaat
    15841 aaaagtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt cggcttcgat
    15901 attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac tctcatctgc
    15961 ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg cttttaccag
    16021 cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg aaaccatcgc
    16081 ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg ccatgacgaa aacgcattaa
    16141 ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag ctgcgacaat
    16201 ttccgggtta tcggcagaaa tggcgctgga aacaacgacc acgctggcat cacgtacgtt
    16261 ttccgggcga tggttgaaat aaatcgtcgc tcccagattc attaactgct gcgtgaccgg
    16321 atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa cttcggcaat
    16381 accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc gacgcatttc
    16441 gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg ccattaactt
    16501 cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc ccggctcact
    16561 tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg ttctgccatg
    16621 gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc cacgctaagc
    16681 tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc attccagtat
    16741 tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc cgcggcgatt
    16801 tcactcaccg ttaaagcacc ggagcggcaa acgacgacat ccgcccacgc atacgccgcc
    16861 gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc ggcatacgcc
    16921 tgttcaacgg attgttgcga acctttaccg ctctgatgcc agatagtgac tgaatcaccc
    16981 agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg ggaaccaccc
    17041 actaccagca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg caacggcagc
    17101 gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa agcgcctgga
    17161 aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa gcccgcaata
    17221 ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag accacctgga
    17281 cctgatacat agccgcccat accgagtacc acgtcaggtt tgtacgcttt cataatcgcc
    17341 cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag ctatcagtgc ttttatacct
    17401 tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt tggcactaag
    17461 tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg agccattaga
    17521 tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc catcaccatt
    17581 aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct ttctccagac
    17641 gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc agtaagctcg
    17701 aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc cccgccgccg
    17761 cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatagaacag gcgagaaaac
    17821 cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat cgacatcgcg cgaaaagcga
    17881 cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct tcgccgataa
    17941 tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt accgagttac
    18001 ctaaaccttg tccccaaagt tcgccgcgac caaacgccat cagcgattgc gttaactgat
    18061 agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg cggatacggt
    18121 acggttcggc gagtatcagc aacacaaccg ctgaaatgcc cataccgata atggcaatga
    18181 actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca aacaccacca
    18241 ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga atcacgccca
    18301 tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc accagatagt
    18361 tggcgatata acaaaacagc gacagttttg tcagctccgc aggctggata cgcagcaaac
    18421 cgagatcgat ccaacgcgat gcccctttaa ccgagctccc cactaccagc acgatcatca
    18481 gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac tccatcggca
    18541 gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca tcacgcttcg
    18601 cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc gaggtcacca
    18661 tgataaagcc aatcgccgca aggccgaagg tcagccacag taaggtgcga tcgtacatga
    18721 tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccttttagc gccgtggaga
    18781 tccagaccag gatactgaat cctggcaggc gcggcatttt caggcgaggg agagataaac
    18841 gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt caaagttctt
    18901 gaactgatca aggctggcac aggccgggga gagcagaacc atatcgcccg gctgaacacg
    18961 cggagccagc aagcgcatcg cctgttccat agtttcggtt tgttctgcca cttccgggcg
    19021 tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac aaacgcacgt tgtcgccatt
    19081 cagatagcgc gccagtggac tgaagtccgc cgatttacca tcgccgccca gcaacaaatg
    19141 cagcgtgccg tctacctgca agccattcaa cgccgcttcc gtactgccga cgttagtggc
    19201 tttcgaatcg ttaatccagc gtacgccatt gtgctccagc accacttcaa agcgatgcgg
    19261 cagaccggtg aaggtggtta acgctttcag gctgctggcg cgcggcaaac cagctgcgtc
    19321 tgccagtgcc agtgctgcca gcgcattggt gtagttatgc tgcccggaaa gtttcatctc
    19381 tttcacgttc agcactttct cgcctttcac tcgcagccag gtttctccct gctgatgatt
    19441 caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatcag caccgcgaat
    19501 cggcattgtt aaggcatcat cagcattgac gacacaaact ttcgcatttt cgtaaatacg
    19561 caattttgcc gcacggtatt gctgcaaacc aaacggatag cggtccatat gatcttcagt
    19621 cacgttcaga atggtcgccg ccaccgcctg taagctggaa gtggtttcca gctggaagct
    19681 cgacagttcc agcacgtaca gttcgcactc agcatcaagc agcatcaacg ctggcaggcc
    19741 aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac ccactagcgt
    19801 ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca atcggtgctt gtgcttcgcg
    19861 gcagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag cgcttaagga
    19921 tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca tcagccattc
    19981 atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt aatttatcca ggccaggcgg
    20041 tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa agtccacgca
    20101 ggaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctgat aatcagccat
    20161 gattaacgta ccttcagcgt tgccagacca atcagaacca gcatcagcga aataatccag
    20221 aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg atgaatcggt
    20281 gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag gatgacagaa
    20341 agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg acgtagcagt
    20401 acggcgataa tgcctaacgc accacctaac gccagcgaac ctacatcgcc cataaagacc
    20461 tgcgccggat aggtgttaaa ccacaggaag cccagtcctg ccccgactat cgcggtacag
    20521 acaataacca gttccccggc gtgtcgcaga tacggtatgt gtaaatagct ggcaaagttc
    20581 atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac ggtcggcata
    20641 attgccaggc cgtcgagacc atcggtcagg tttaccgcgt tgccagtacc cacaatgacg
    20701 aagtaagcca gcagaatgta gaacagcccc aactgcggca tcacatcttt aaagaatggg
    20761 accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagggcgaa ggcgacaccc
    20821 agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt ggtgtcttta
    20881 cgcaccactt tgcgataatc atcaacaaag ccaataacac cgtaacctac cagcaccacc
    20941 aacacgcacc agacgtacgg attggacggg taagcccaca gcagtacgga gatcacaatc
    21001 gccgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa gtgtgattca
    21061 ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc aatcatacgc
    21121 gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc gcgaaacgtc
    21181 agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc cagccaaact
    21241 aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca ctacgtgaac
    21301 ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga cgcgtaatta
    21361 acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc ggtgctgata gcatggcttt
    21421 gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcctcg cccacctgta
    21481 catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc agcacgcggt
    21541 agcccggcat ttcagccagt acctgaacag cggcggtcat tgaaccgaca ttggcgttgt
    21601 aggagtcgtc gagcagcaac tggttttctg ccagttggat ggggaacaga cggcctggaa
    21661 cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc acggacatgg
    21721 agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac ggcagcagaa
    21781 catcgacgct accggttggg gtttgtaggg taaattccgt accgtgcgag gtcacatgga
    21841 tattggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac actttgcgtg
    21901 agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata atggcgatac
    21961 cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc gcaagcgagc
    22021 caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgcagcttcc gggcgagtca
    22081 gactcacagt ccaggctatt tcgccctgat ggttcgcgcc aagttcaata actgcgtaat
    22141 cgtattccgg cgttaagcgc aacaacgtca tcggtacacc gatgtcgttg ttgagattgc
    22201 ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc tctttaacgg
    22261 aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc cggaacttgc tggcgaaccc
    22321 atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc ggcaggtcga
    22381 tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc agctttcgcc tggtcggcaa
    22441 aatcgtgggc atcaaaacgt tcgcctttca gggcaacaaa caggcagccc ggcgtcagtt
    22501 ttcgggtatc agtggttaca gcatcaaggg tgatatctgc accttgcagt tcaccgttga
    22561 gaatgtcggt aagttggcta agggttacgc taatcatgca atcaccccca gcagacgcgc
    22621 caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt aatcttcatg
    22681 gcctttgccc gcgaccagca ccacatcgtt ctctttagcc tgcataacgg cgcaagtcac
    22741 cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc ccgccagaat
    22801 atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca ccaccgccac
    22861 gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc ttacctttat cgcgatcgcc
    22921 accacagcca aagacacacc acagcttgcc cgcacagtgc agacgcgccg cctgtaaggc
    22981 tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt tgcctggcac
    23041 agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt tcaacagatc
    23101 agccaatgga tagccgagtg ccaacagtgt cgccagcgcg agcagcaggt tgctgacgtt
    23161 aaaagcgcct atcagatggc tttcaatttc gccatcgccc caacttgagc taaagcgaat
    23221 cgtcacaccg ctgtcgtgat agttcacgtc gatcactttt aaccagcgtc cgtgacagtt
    23281 cggattaata tgatcttcca ttgataccgc aaccgcgtcc ggcagctttg ccagccaacg
    23341 gcggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgtt cagaataaag
    23401 cagccatttc gcagcttcgt agtgttccat atcaccatga taatcaaggt gatcgcggct
    23461 taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct gtaccagccc
    23521 gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc tccaccagcc ccgccagctc
    23581 atgctgaaca tcgactgccg aaccggtggt attttctgtc gggatcactt tccccagcag
    23641 accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc tccactgcgc
    23701 cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt acgcctacga gacgtaaatt
    23761 gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt tgagctggct
    23821 gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg cctcatcttt
    23881 cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat atcgacgccc
    23941 gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca cacggctgtc
    24001 gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag caagaaggtc
    24061 gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat tcatttttat
    24121 cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg cccatgatgg
    24181 caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc ggatcgttga
    24241 taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg gtataagcaa
    24301 tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg gttttaatgg
    24361 cgatacgata gcctttaatc gccgccttca cgccgccgcc gcccggtagc gccacgcttt
    24421 ccatcatatg caccacggtg cggacaatgg attccgggaa gacacgttca ccgggaaccg
    24481 gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg atagttgcgt
    24541 agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag aaggtggccc
    24601 tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct ccgaccaacc
    24661 ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac gctgaggacg
    24721 gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat accccggtca
    24781 gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt cgataaggaa
    24841 tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc atcaccacca
    24901 tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta cgcatcgcct
    24961 ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc atcgccagca
    25021 cttcacctgt gttgacatcc accagcacgg cgctaccaga ttcagccttg ttaaaggcca
    25081 ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata ctcagcgcca
    25141 ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga ccatagcggt
    25201 ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa ctcttctcaa
    25261 cgccctcaat cccttgacta tcgacgttag taaagccaat gaggtgagca gtcacttcgc
    25321 cggatggata ataacggcga gattcttcac gcagatgaat acccggcagt ttcagttttt
    25381 tgatgtagtc cgccatgtca gggtttacct gacgcgccag ataaataaag cgccctttcg
    25441 ggttggcgtt aatgcgggca gaaagctgat ccagcggaat attgagcgcg ttagccagcg
    25501 ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc gggtcagccc
    25561 aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca gtaatcatgc
    25621 cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct ttcaccagca
    25681 tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc agcgccagga
    25741 gaatacagcc gcataacaac gcaaaacgcc aactaataaa gttggcatgt tcttcctgac
    25801 gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct tatttttgca
    25861 ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc gtggcgatcc
    25921 tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg cgccattcaa
    25981 tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt aaacgggtat
    26041 ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac aggcagagtg
    26101 gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca tggcgctcgt
    26161 ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg atcatgcgtt
    26221 cgtcctctct gcgatacgca gaactgaact acgggcacga ggattctcag ccacctcttc
    26281 ttcgcccggc attaacttgc ctaatgctcg cagctgacgg ccacccagtt ttttgagctg
    26341 ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt cacgcataaa
    26401 acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc gcccacccgg
    26461 ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt cactgtttac
    26521 ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat ctttcaccgg
    26581 cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct gttcgcggtt
    26641 acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat aggttttcaa
    26701 tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg actgcccacg
    26761 ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc cacgttcagc
    26821 atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga tcttgccgat
    26881 aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga tgatggagaa
    26941 gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat cgatcgccag
    27001 caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac cgcgaccaaa
    27061 agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg cttcatccag
    27121 cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc tgcagtcgct
    27181 ccgataagtc tccagtagcc aactgctctg cgtcgatatc ttccttgacc tgttgatgcc
    27241 aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc acttcttttg
    27301 tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg ccatccatct
    27361 gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaact gggttcatgc
    27421 tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg taaagcagca
    27481 ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc tcaagcagct
    27541 gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg actaacgttg
    27601 ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat ttcccactga
    27661 aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat accgaggtaa
    27721 aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac gcgcgaagca
    27781 aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcactg ttttaaaatg
    27841 actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg gcattttagg
    27901 acatatcttc cccggttaac agtccttgtt acgtctgtgt ggcgcttgat gccagcgggt
    27961 ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatcgccgc atgtaacaaa
    28021 ataatgcaaa aacgcgctgt cgatatcact taattattgt gaatttcagc tggtgagtta
    28081 attaacgggc gggtttaaaa ccatttatca ataatagtca ggaatagtct tatttacttt
    28141 aagcatattg atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta
    28201 tttttgttcg cggcttagct acggctgagc acaccgcggc gatagagatt acgtttaatg
    28261 cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc
    28321 acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccgggca ctgtaagaag
    28381 tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttaccatcg
    28441 cgacgtagcg tgacatccat caccccttgc aacaacgcaa acgacgttgt gaacagcgcc
    28501 tgcggcatca gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca
    28561 tagctgttgg catacaggaa atgcacttcg cgcggatcat ctttccaggc agtacggaaa
    28621 ccttgttcac gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc
    28681 tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca
    28741 accacgctgg tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg
    28801 gcgttagccc agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg
    28861 gcatcaacct gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct
    28921 tctgagcagg caatcagcag ttgataaccc cgttgccgcg cctggcgctc aagatagtta
    28981 gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg
    29041 cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca
    29101 gccatgactt tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata
    29161 acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca
    29221 attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat
    29281 taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat
    29341 ttcgcaaaaa aagcccagcg tgacatgttg ggctgcaaat tgcacactga gatcattctc
    29401 aacgcattat tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa
    29461 ttttcgccac ctcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta
    29521 attgaacggt ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat
    29581 tacgtttcac ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc
    29641 gctcaacatg cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta
    29701 attgcttttc gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg
    29761 ataatgtcgg atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg
    29821 aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata
    29881 tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc
    29941 gcgaatctgc atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag
    30001 gcgattattg cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg
    30061 agagatctgg atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga
    30121 ttgcatataa gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat
    30181 ccccagatag cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt
    30241 agacagttcc tgaatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc
    30301 ttctggcaac gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag
    30361 gccaccggaa ttgatccagc gacgcggttt gtcgaatgga taataaagtg cagcaaacat
    30421 ctggtgctgc ccgacatcgg acgtcacgta agcgtctccc ttcgtcaacc gccaaagagt
    30481 ctcgatcacc gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgacg
    30541 agcgcgccac tgttcaattt gctgccacca gtcgcggatc tcatccagtg gttgatgggc
    30601 ggattcttgc gacaagagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat
    30661 cgggatatcc gcagtcacgg ttttagaaat ggaaatagga tcaatatcga tatgcagaac
    30721 agtggcattt gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc
    30781 gacggcgaaa atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat
    30841 tcccagcatg cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga
    30901 ggaaacaacg ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc
    30961 catgattgcc ccaccgccta cgtagacaac cggttttttt gccactacca gcgtttgcag
    31021 agcacgctta atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac
    31081 cgactccggc cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc
    31141 aacgactact ggtccaggac gaccacttgc cgccagccag aaagcctttt tcagcacctg
    31201 cggaatgtct tccgtttgct taaccagaaa actgtgttta accactggtc gcgaaatccc
    31261 caccatgtcg cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga
    31321 aaggacaact aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt
    31381 cgcccctgga cccgacgtta ccagcacgac gccgacttcc ccggtcgcgc gcgccagacc
    31441 atcggccata tgcactgccg cctgctcatg acgaactaaa acatgatcga taccacccac
    31501 ggtatgcaat gcatcgtaaa tatcaaggac tgcgcctcca ggataaccga atacttgttt
    31561 aacgccctga tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc
    31621 ctgcctcact gtttgatgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa
    31681 gcaattggca gccagagaca taaacgcagg gtaataagaa taaacaggag gaaagatgaa
    31741 aagaaacgat aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc
    31801 acaacttaca gcaatttctc tattttttta attaatccta aaatagagag cccacacatt
    31861 cagaataaaa ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctgacc
    31921 tattctgcat tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg
    31981 cctttatcgc gcccggcagc ttcatgccat gagagataac aggttctgct gttttgtttt
    32041 aacggcagcg gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc
    32101 ggcgcaatag cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg
    32161 ccctgatacg cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac
    32221 gcgaaacgat cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac
    32281 gggcctttaa ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat
    32341 ggcacgctgg taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc
    32401 tgataacgca gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata
    32461 tttggcgcaa tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg
    32521 ctgcaaacac aaagatgaaa tacacgttca ctactcgcgg gttcaaaacc tgaaccaggc
    32581 aattcatttt gtactagttg caatgcctgg cgaactgaac caaaaagttg
//
LOCUS       DAJTYD010000029        31309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTYD010000029 DAJTYD010000000
VERSION     DAJTYD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.029"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..674
                     /gene="panC"
                     /locus_tag="OO585_001211"
     CDS             <1..674
                     /gene="panC"
                     /locus_tag="OO585_001211"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6628866.1"
                     /translation="QFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNGTE
                     THTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIRKM
                     VADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAGER
                     DLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLIDNK
                     IVELA"
     gene            complement(966..1862)
                     /gene="rpnC"
                     /locus_tag="OO585_001212"
     CDS             complement(966..1862)
                     /gene="rpnC"
                     /locus_tag="OO585_001212"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6628867.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            2136..2516
                     /gene="panD"
                     /locus_tag="OO585_001213"
     CDS             2136..2516
                     /gene="panD"
                     /locus_tag="OO585_001213"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6628868.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(2520..3720)
                     /locus_tag="OO585_001214"
                     /pseudo
     CDS             complement(2520..3720)
                     /locus_tag="OO585_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(3815..4255)
                     /locus_tag="OO585_001215"
     CDS             complement(3815..4255)
                     /locus_tag="OO585_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6628869.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(4360..5130)
                     /locus_tag="OO585_001216"
     CDS             complement(4360..5130)
                     /locus_tag="OO585_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6628870.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(5127..6053)
                     /locus_tag="OO585_001217"
     CDS             complement(5127..6053)
                     /locus_tag="OO585_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6628871.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            6162..6824
                     /gene="can"
                     /locus_tag="OO585_001218"
     CDS             6162..6824
                     /gene="can"
                     /locus_tag="OO585_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6628872.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(6865..7401)
                     /gene="hpt"
                     /locus_tag="OO585_001219"
     CDS             complement(6865..7401)
                     /gene="hpt"
                     /locus_tag="OO585_001219"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6628873.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            7607..9997
                     /gene="gcd"
                     /locus_tag="OO585_001220"
     CDS             7607..9997
                     /gene="gcd"
                     /locus_tag="OO585_001220"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6628874.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(10044..11594)
                     /gene="cueO"
                     /locus_tag="OO585_001221"
     CDS             complement(10044..11594)
                     /gene="cueO"
                     /locus_tag="OO585_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6628875.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            11760..12107
                     /locus_tag="OO585_001222"
     CDS             11760..12107
                     /locus_tag="OO585_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6628876.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            12213..13079
                     /gene="speE"
                     /locus_tag="OO585_001223"
     CDS             12213..13079
                     /gene="speE"
                     /locus_tag="OO585_001223"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6628877.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            13095..13889
                     /gene="speD"
                     /locus_tag="OO585_001224"
     CDS             13095..13889
                     /gene="speD"
                     /locus_tag="OO585_001224"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6628878.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(13926..14288)
                     /locus_tag="OO585_001225"
     CDS             complement(13926..14288)
                     /locus_tag="OO585_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6628879.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(14463..17060)
                     /gene="acnB"
                     /locus_tag="OO585_001226"
     CDS             complement(14463..17060)
                     /gene="acnB"
                     /locus_tag="OO585_001226"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6628880.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGLLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFMNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            17415..19268
                     /locus_tag="OO585_001227"
     CDS             17415..19268
                     /locus_tag="OO585_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6628881.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(19339..20763)
                     /gene="lpdA"
                     /locus_tag="OO585_001228"
     CDS             complement(19339..20763)
                     /gene="lpdA"
                     /locus_tag="OO585_001228"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6628882.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(21088..22980)
                     /gene="aceF"
                     /locus_tag="OO585_001229"
     CDS             complement(21088..22980)
                     /gene="aceF"
                     /locus_tag="OO585_001229"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6628883.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(22995..25658)
                     /gene="aceE"
                     /locus_tag="OO585_001230"
     CDS             complement(22995..25658)
                     /gene="aceE"
                     /locus_tag="OO585_001230"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6628884.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(25819..26583)
                     /gene="pdhR"
                     /locus_tag="OO585_001231"
     CDS             complement(25819..26583)
                     /gene="pdhR"
                     /locus_tag="OO585_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6628885.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            27124..28497
                     /gene="aroP"
                     /locus_tag="OO585_001232"
     CDS             27124..28497
                     /gene="aroP"
                     /locus_tag="OO585_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6628886.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(28540..29394)
                     /gene="ampE"
                     /locus_tag="OO585_001233"
     CDS             complement(28540..29394)
                     /gene="ampE"
                     /locus_tag="OO585_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6628887.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(29391..29942)
                     /gene="ampD"
                     /locus_tag="OO585_001234"
     CDS             complement(29391..29942)
                     /gene="ampD"
                     /locus_tag="OO585_001234"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6628888.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            30030..30923
                     /gene="nadC"
                     /locus_tag="OO585_001235"
     CDS             30030..30923
                     /gene="nadC"
                     /locus_tag="OO585_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6628889.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            31126..>31309
                     /locus_tag="OO585_001236"
     CDS             31126..>31309
                     /locus_tag="OO585_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6628890.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHG"
BASE COUNT         7549 a         8517 c         7687 g         7556 t
ORIGIN      
        1 tgcagttcga ccgcccggaa gatctggcac gttatccacg gaccttgcag gaggactgcg
       61 agaagctaaa caaacgtaaa gtggatttag ttttcgcccc ttcggtaaaa gagatctacc
      121 cgaacggtac tgaaacccat acttacgttg acgttcccgg gctttcgacc atgctggaag
      181 gcgcgagccg tccggggcat tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc
      241 tggtccagcc ggacatcgcc tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc
      301 gcaaaatggt tgccgatatg ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg
      361 ccaaagatgg ccttgcgctc agttcccgta atggttatct gacggcggaa caacgcaaaa
      421 ttgcgcccgg tctgtacaaa gttttaagtt caattgccga caaattgcag gctggcgagc
      481 gggatcttga tgaaattatt gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg
      541 ccgatgatat tcagattcgc gatgccgaca cgttgctgga agtctctgaa accagcaaac
      601 gagctgtaat tctggtagcc gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg
      661 tcgaactggc gtaatgcttt tactctaaat aattcaagtt gcaggaaggc ggcaagcgag
      721 tgacaaattc gtcaggaacg aatttgccca gccgcaggct ggccttcggt gagagacatg
      781 gatgtctctc attaatcccc aggagcttac ataagtaagt gactggggtt agcgaacgca
      841 gacacagcac atgcaattta aagtatgacg agtataactg gcgctacggc tgatggcgcc
      901 aattattaat ttgccccacg tcatggcgat ggcgtctgta agcaattatt cataacgcag
      961 acaacttagt taatcagcct atcaatctca gcaagagata aatttgtcat ctctgcaacg
     1021 tcttcccgag acatcccttt actcagaaaa cgctgggcga attcttgcct ttcctcctgt
     1081 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     1141 atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     1201 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     1261 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     1321 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     1381 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc cagctccggc
     1441 gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     1501 aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg ccggtgcatg
     1561 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gccttggtgt
     1621 tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata gagcacgtcc
     1681 gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     1741 aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     1801 tccgcgtgca ttaaaaattg tttaaacact gcatcatgcg gtgtggtact cggtgcatcc
     1861 atcttccctg ccttccgtgt tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc
     1921 gtcagccacc tctttagtgt ttaccgagca gcgttcagag aaatttcaat tggttacagc
     1981 cgttacatgg cacgcttcgg cacggtacgc aaactgctga tgtaaatgcc gtcagatgag
     2041 tagacactaa acaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc
     2101 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     2161 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     2221 accaggattt tcttgacgca gccggtattc tcgaaaacga agccattgat atctggaatg
     2281 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     2341 tttctgttaa cggtgcggcg gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca
     2401 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaacgtc gcctattttg
     2461 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     2521 tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc cgttcttaag
     2581 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     2641 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaatccg
     2701 ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa
     2761 cgtgcaaaat caaacagaat attgtgttca ctacggctcg gtaatatggg gcggcgataa
     2821 ccatccaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     2881 ttaagttcag aaacgctcat aaattgcagc gattttgggt cccacttctg cgggtgacgt
     2941 ttgatgcgcg aggtaacaat aaacgccgtc gccttcatgc catattgttt caacacggga
     3001 tacgcatagc ggctcaccga tttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     3061 gggagattga tcttattctt cacgtaacct tccagctgca ccatgctcag tgtcgcgtat
     3121 cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa
     3181 gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt tagcaccggc
     3241 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     3301 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     3361 ttgtccgcca gtaccccata atggcgcact gcccgcactc ggcgcgttat agaccggcgt
     3421 atctttccag gtaattaagt tctgattact cagcggcttg ttgaggtcac ccaaaccgtc
     3481 ttcaactttt tgtcgcccct gaactggctc gagataccct ttatcgataa accctttacc
     3541 aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc tccacggcaa tgatttgccc
     3601 ggcccgaata ttccccacgg tcaccatctt gtcgccaatt tgcgcccaga tcgcagcatt
     3661 ttcgatggtt tgcatataac gggcaggtaa cgcggcacta acgcttgcgg taaacagcat
     3721 cagcaggatg aataacagct tgtttataca tgaaaaaata accgagggtg aaacaacgct
     3781 tgtattgtag caaaagcgcc atcagaatcg cgggttaata ctcatacaaa ttatgtttga
     3841 ggacaaaagg cggatttttt tgttgttggt gccaaagact actcacctcc ggcgcaccca
     3901 gttcgacaat acgctcgcga aactctgaac tggtcatggt ttcacggcaa gccatcatct
     3961 gttcaattaa cggtaacgtg acaccagaaa tcacttcaca acgggagtgt ttatggctta
     4021 ataatgaagc cacgcggtac ggtggcgctc cggctatatc ggttaagaag atgacaccct
     4081 caccagagtc cgcttcatgc agagcatcgc acatcatgcg gctgagcata ttagagctta
     4141 atccgcgcca gaaattcacg gcccggcact gaagaagtgc cccatgtttt ttctccagcg
     4201 catccagtat ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct aacatcgcct
     4261 tcctccttag ccgaaaatca gtctatcctg accattgttg ataaagggtg atagccgtca
     4321 aaactcctct cccccgaatc cagaggagag gaaatagcct tagctacgca aaccacgtcc
     4381 acgttggatc agcgaccaac agatcaaata aaacaccaca ataaagacca ccagtacgcc
     4441 aaaggtagtg gccagcggca catcatttat accgaggaag ccgtagcgga aaccactgat
     4501 catataaacg attgggttca ggtgcgacag cccttgccag aacggcggca acaaagtcag
     4561 ggagtaaaac accccgccca aatacgtgag tggcgttaac acaaaggttg gcaccaggct
     4621 gatgtcatcg aacgttttgg caaacacgcc gttcagcaaa cccgcaaggg agaatagcac
     4681 cgccgtgagc accagggtta aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa
     4741 aaacagtgaa attgccgtca ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc
     4801 gacatatccg gcaataatga cgtgagtcgg aaccggcgct accagcagct cttcaatatt
     4861 acgctggaac ttggcaccaa aaaatgatga cgcgacgtta gcgtaggcat tggtgatcac
     4921 cgacatcatg atcagccccg gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc
     4981 aatacgcgaa cctatcaggt taccgaagat aataaagtaa agggtcatgg tgatgactgg
     5041 cggcaccagc gtctgcaccc agatacgcat aaagcgatgg atctctttcg cccagatgct
     5101 ttttagcgcc acccagtaaa gatgcatcat gcgcgatctc cttgtttttc attaaccagt
     5161 gaaacaaaca gctcttccag acggttagct ttgttacgca tacttaatac ctgaatgccc
     5221 tgctcactta actgcgtaaa tacgctgttg atcccctgct cacgcagcac ttcaacttcc
     5281 agcgtcgcgg tatcgaccag tcgatactga tagccatcga gcttcggtaa cgggcttttc
     5341 ggtgcgagat cgagaataaa ggtttccaat ttcagcttcg ccagcagcgc cttcatcgag
     5401 gtattttcca ccagctcacc gtgttgaata atgccgatat tgcggcacag catttctgct
     5461 tcttccaggt agtgtgtagt gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa
     5521 aagccccaca ttgagcggcg aagttcaata tccacgcctg cggtcggttc gtcgagaatc
     5581 agtagtttag gttcatgcat taacgcacgg gcaatcatta aacggcgctt catcccgcca
     5641 gataacatac gcgcacgttc gttgcgtttt ccccataaat cgagttgttt gagatatttt
     5701 tcgctgcgga tgtacgcttc tttgcgctcc acgccgtagt accctgcctg attcaccaca
     5761 atttgctgca cggtttcaaa cgggttgaag ttaaattcct gcggcaccag tcccaactga
     5821 cgtttagcgt tcacgacatc cttctcgaga tcgtaaccaa atacgctgac ccgcccggag
     5881 gttttattta ccagagagct gataataccg atagtggtcg atttcccggc cccgttcggc
     5941 ccgagaagcg cataaaaatc acccgcttcg acctgcaaat ctattccacg aagcgcctga
     6001 acgccgcctg gataggtttt tttaagctgt tgaagttcca gtgcaatggt cataaatttt
     6061 tacttacctt acgttcttac actttatctg tggtttaaat cgtcccggag ttgccctata
     6121 ttagccaaac gtaattattt ggttacaggt cgttaacctc catgaaagac atagatacac
     6181 tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga agaggatccc gggttttttg
     6241 agaaactggc acaagcgcaa aaaccgcact ttctatggat tggatgttcc gacagtcgcg
     6301 ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg
     6361 ctaacctggt cattcacacc gacctgaact gcctttccgt ggttcagtat gcagtggatg
     6421 tactagaagt tgaacacatt attatctgtg gccactacgg ttgcggcggc gtacaagccg
     6481 cagttgaaaa cccggaactg gggcttatca acaactggct gctgcatatc cgcgatatct
     6541 ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt
     6601 gtgaactgaa cgtcatggaa caggtgtata acctaggcca ctccaccatt atgcaatcag
     6661 cgtggaaacg cgggcagaaa gttaccattc acggctgggc ctatggcatt cacgacggct
     6721 tgctgcgtga tctggatgtt actgccacca accgcgaaac ccttgagcaa cgttaccgtc
     6781 acgggatttc caacctcaag ctgaaacacg ccaaccacaa ataaaaatgc catgccggat
     6841 gcaacacatc cggcaacttc acacttactc gtccagcaga atcactttgc cgatatacgg
     6901 cagatgacgg taacgctgtg cgtaatcaat gccgtaaccc accacaaact catccgggat
     6961 cgagaaaccg ataaattcta ccgggacgtt cacttcacga cgggacggtt tatccagcag
     7021 cgtacaaatc gccagcgact tcggttcgcg caggcttaag atctcacgca ctttcgacag
     7081 tgtattcccc gagtcgatga tatcttcaac aatcagcacg tccttgccac ggatatcttc
     7141 atccagatct ttgaggattt tcacatcacg ggtggtggac atgccgctac cgtagctgga
     7201 ggcggtcata aagtcgactt catgagatac ctgaacttca cggcacaggt ccgccataaa
     7261 cataaatgag ccacgcagca gacccaccag caccatatcg ctgccgctgt ctttgtaacg
     7321 ctcagtaatc tgacgaccca gttcggcgat acgcgcttta atctccgctt cggggatcat
     7381 tacttctaca gtatgtttca tatctctaac catatgattt aaaaacaaat cacttcgcac
     7441 gggcaggttt tggcaccgca acaaagcgca agacacgcag tataccagta aaactattca
     7501 ttcgcggaat ctgttaataa agctcgaatt gtgatgacga tcacacatgt taaaacctat
     7561 aattaattgc tactaaaata ttaatgaatt gaaatggtgt ctctttatgg caattaacaa
     7621 tacaggctcg cgacgattac tcgtcacgct aacagtcctt tttgcagcgc tttgcgggct
     7681 gtatctactc attggcggag gctggctggt cgcgattggc ggctcctggt actaccctat
     7741 cgctggcctt gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct
     7801 ttggctatac gccgccctgc tgctcggcac catgatttgg ggcgtctggg aagttggttt
     7861 cgacttctgg gcgctgactc cgcgcagcga tattctggtc ttcttcggca tctggctgat
     7921 cctgccgttt gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg ccgcactggt
     7981 ggtggcactg ctgattagcg gtagtatcct gacctgggcc ggatttaacg atccgcagga
     8041 aatcaacggc accttaagcg ccgatgccac acctacggaa gctatctcac ccgttgccga
     8101 tcaggactgg cctgcctatg gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca
     8161 aattaacgcc gataacgtcc ataatctgaa agaagcctgg gtgttccgta ctggcgatgt
     8221 gaagcagccg aacgatccgg gcgaaatcac caatgaagtg acgccgatta aagtgggcga
     8281 caccctttac ctgtgtaccg ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa
     8341 agagaaatgg cattacgatc ctaagctgaa aactaacgag tctttccagc acgtaacctg
     8401 ccgtggcgtc tcttatcatg aagccaaagc agaaacggct tcgccggaag tgatggcgga
     8461 ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcga ctgattgcga ttaacgctga
     8521 aaacggcaaa ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat
     8581 gccagacacc aaaccgggtc tgtatgaacc gacttcgcca ccgattatca ccgataaaac
     8641 catcgtgatg gcaggttcag ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat
     8701 ccgtggtttt gatgtcaaca ccggggagct gctgtgggct tttgatcccg gcgcgaaaga
     8761 tccgaacgca atcccgtctg acgaacacac ctttaccttt aactcgccaa actcttgggc
     8821 accagcggcc tatgacgcga agctggatct ggtctatctg ccgatgggcg tgaccacgcc
     8881 ggatatctgg ggcggtaacc gcacaccgga acaggaacgt tatgccagct cgattctggc
     8941 gctgaatgcc actaccggga aactggcgtg gagctaccag accgttcacc acgacctgtg
     9001 ggacatggat cttccggcac agccgacgct ggcggacatc accgttaatg gtcagaaagt
     9061 gccagttatt tacgctccgg cgaaaaccgg caacattttt gtgctcgatc gtcgcaatgg
     9121 cgaactggtg gttccggccc cggaaaaacc ggtgccgcaa ggtgctgcga aaggcgatta
     9181 cgttactccg acccagccgt tctctgaact gagcttccgt ccgaagaaag atttgagcgg
     9241 tgcggatatg tggggagcca ccatgtttga ccaactggtg tgccgcgtga tgttccacca
     9301 gatgcgctat gaaggcattt tcacgccgcc atctgaacag ggcacgctgg tcttcccggg
     9361 taacctggga atgtttgaat ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat
     9421 tgccaaccca atggcactgc cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc
     9481 gatggagcag ccgaaagatg ccaaaggcac gggtacggaa tccggcattc agccacagta
     9541 cggtgtaccg tatggtgtca cgctcaaccc gttcctctca ccgtttggtc tgccgtgtaa
     9601 acagccagca tggggttata tctcggcgct ggatctgaaa accaatgaag tggtgtggaa
     9661 gaaacgtatt ggtacgccgc aggacagtat gccgttcccg atgcctgtac cggtgccgtt
     9721 caatatgggt atgccgatgc tgggcgggcc aatctccacg gcgggtaacg tgctgtttat
     9781 cgccgctacg gcagataact acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg
     9841 gcagggtcgt ttaccagcgg gtggtcaggc gacgccaatg acctatgaag tgaatggtaa
     9901 gcagtatgtg gtgatctccg caggcggtca cggttcattt ggtacgaaga tgggcgacta
     9961 tattgtggct tatgccctgc cggatgatgt gaaataacca taatgcccgg agagatccgg
    10021 gcatatttcc gaatacggtc tttttatacc gtaaacccta acatcatccc cgtatcttca
    10081 tgctccagca gatggcagtg cgccatataa gcatgttctt tcggtgcgtc gtgattaaac
    10141 ttcaccagca cttcactgac attgccttct accttaacgg tatctttcca gcccgcgcga
    10201 tgagccgctg gcggtttgcc attttctgac aagatacgga actgcgtgcc gtgaatatgg
    10261 aacggatgca gcatcatgtc gcccacgcca gagataaccc aacgttcgta ctgccctttc
    10321 gccgccgcaa acatcggctt attcatatca aacgcctgac cgttgatttt attggcatgg
    10381 tggaaatcga acttcccgcc gtggttcata tgattcatat taccgtgccc catatggccc
    10441 atcatctggc tgtgatccat tccggccatc gcctgatcgc catatttctc cattagcatc
    10501 tgcataccca tcatatcgag catcgggtcc atagaaagtt gcagcttgcg taccgtcagc
    10561 ccttccagcg aaggtaacgc aggcaggcta cttaatgtgt ctggcaaagc accggaggca
    10621 ctaatagcaa tcggctgaat ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc
    10681 atccccatct ggctgaccgg tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc
    10741 accagcactt caaaacgctc gcccatcagc accggcagct cgctcacctt caccggttca
    10801 ggtagcagac caccgtcgct ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa
    10861 ttgagcgagc gggcattaca gccattgagc aaacgcaggc gcagccaacc acgcggggca
    10921 gcgtgttgtg ggtagattgc accgttggtc agcagcgtat cgccaaacca gcccacggcg
    10981 gcggtcatca catccagttg ataatcaatc tgcccatcag cgttaaattt tttatcctga
    11041 acaatcaccg gaacatcatc gataccccac tgttttggca gcattaattt caggatctcg
    11101 tcatcttcaa ttaccaccag cccagccaac cccatcgcca cctgtcgccc agtcttgcca
    11161 tgttgatgtg gatggaacca gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag
    11221 cgcttgccac ctggcggaat aattccctgc gggccgccgt cgacttcacc cggtacttcc
    11281 agcccgtgcc agtgcaacgt cgtctcttcc gtcagttggt tatagatatc aaccgttacc
    11341 gctttgccgc gctgtaattt caccgccggc cccagcagat tgccgttata gccccaggta
    11401 gttgcagttt tctcgccaaa ggtagactga cctgcgccaa tagttaactg aatgcgatta
    11461 cgggcatcgg tcgtgagcaa atcagggatc ggtaacgttg ggcgttccgc cgcaaagact
    11521 gcgcggctcc acagcggcaa ggctgaagcc acacccagcg cgacggaata ttttaagaaa
    11581 tcacgacgtt gcatagttat ttccttattc ttaagcaggc gaaacaaaat caaacgttga
    11641 gcatagtcct tccccttacg ggaaggtcaa gccgcagaca caatttaatc gacaataatg
    11701 gtcgtcgcct cgctcgcagt gtgctaacgt ttatcttctt taagcccctg gtagaagcaa
    11761 tgaagacgtt tttcagaaca gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt
    11821 acgcgctcag cgagtctgaa gccgaagata tggccgattt aacggcagtt tttgtctttc
    11881 tgaagaacga ttgtggttac cagaacttac ctaacgggca aattcgtcgc gcactggtct
    11941 ttttcgccca gcaaaaccag tgggatctca gtaattacga caccttcgac atgaaagccc
    12001 tcggtgaaga cagctaccgc gatctcagcg gcattggcat tcccgtcgct aaaaagtgca
    12061 aagccttggc ccgcgattcc ttaagcctgc ttgcctacgt caaataatcc ctgatacctt
    12121 tttgtagaaa tattgaccgt gcatccgcgg tcaatgttag ctattatgtt gcgccctttt
    12181 tttacgggtg ttaacaaagg aggtatcaac ccatggccga aaaaaaacag tggcatgaaa
    12241 cgctacacga ccagtttggg cagtactttg cggtagataa cgttctgtat catgaaaaga
    12301 ccgatcacca agatctgatc atttttgaga acgctgcatt tggtcgcgta atggcgctgg
    12361 atggcgtagt acaaaccacc gagcgcgacg agtttatcta tcatgagatg atgacccatg
    12421 ttccgctact ggcccacggt cacgcgaaac atgtgctgat tatcggcggc ggcgacggtg
    12481 ccatgctgcg tgaagtaacc cgacataaaa acgttgagtc aatcacgatg gtggaaatcg
    12541 atgcgggtgt cgtgtcgttc tgccgtcagt atctgcccaa ccataacgcc ggtagctacg
    12601 acgatccgcg ctttaagctg gtgatcgacg atggcgtcaa tttcgttaat caaaccagcc
    12661 agacctttga tgtcattatc tccgactgca ccgatcctat cggtcccggc gaaagtctgt
    12721 tcacctcagc attttatgaa ggctgcaaac gttgcctgaa tcctagcggt atcttcgtcg
    12781 cacaaaacgg cgtctgcttt ttacagcagg aagaagccat cgacagccat cgcaaactca
    12841 gccattactt cagcgacgtt ggcttttatc aggcggcgat cccgacctat tacggcggta
    12901 tcatgacttt tgcatgggcg acagataacg acgccttacg ccatctctca accgaaatta
    12961 ttcaggcgcg ttttctcgct tctggcctga aatgccgtta ttacaatccg gcaatccata
    13021 cggcagcttt tgccttacct cagtatctgc aagatgcact ggcttcacag ccgtcctaag
    13081 gagaagataa gaaattgaaa aaactgaaac tgcatggctt taataatctg accaaaagtc
    13141 tgagtttttg tatttacgat atctgctacg ccaaaactgc cgaagagcgc gacggttata
    13201 ttgcttatat cgatgaactc tataatgcca accgtctgac cgaaatcctg tcagaaacct
    13261 gttccattat cggggccaat attcttaaca tcgcccgcca ggattacgaa ccacagggtg
    13321 ccagcgtcac tattctggtg agtgaagaac cggttgaccc gaaactcatc gacaaaacag
    13381 aacaccccgg cccactgcca gaaacggtcg ttgcccatct cgataaaagt catatttgcg
    13441 tacataccta cccggaaagc catcctgaag gtggtttatg taccttccgc gccgatattg
    13501 aagtctctac ctgcggcgtg atttctccgc tgaaggcgct gaattacctg atccaccagc
    13561 ttgagtccga tatcgtaacc attgattatc gcgtgcgcgg ttttacccgc gacattaacg
    13621 gtatgaagca ctttatcgac catgagatta attcgattca gaactttatg tctgacgata
    13681 tgaaggcgct gtatgacatg gtggatgtaa acgtctatca ggaaaatatc ttccatacca
    13741 agatgttgct taaagagttc gaccttaagc actacatgtt ccacaccaaa ccggaagatt
    13801 taaccgacag cgagcgccag gaaattaccg ctgcgctgtg gaaagaaatg cgcgagattt
    13861 attacgggcg caatatgcca gctgtttaac ggctctggcg gagctccagg ctccgccaga
    13921 tttatttact tctgctgcac gaaattgcgg taagccgcca cgacctgcag aaaatcctca
    13981 acgccgcata gcgacaggct ttcttcgtcg tagtagttca tcccctcttc catttcatcg
    14041 ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt caccgtccat ccacagggtg
    14101 tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt tcagtgcgtg cgctgcttgt
    14161 tccacttcat caagcaaggc caggttttct ttcacctctt cattaaacca gtgcccgacc
    14221 acttcatgcc ccatggacat acgcaccttt accactccgg taatatcgcg cagaaattcg
    14281 taatccatag tgtgttcctc tgctcccggc aatgcgccga aatcgcattg cgccattgat
    14341 gtaattatcg cagccttacc ggagaaaaac agcggaacgg cgatggttta gaaataaaaa
    14401 atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact
    14461 tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg agaaagttgg
    14521 ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc aacgtaggtc
    14581 tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc cagttctgca
    14641 gaagccagga agacattcgc gccagtcccc agacggttcg ggaagttacg ggtagaggtg
    14701 gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag agaacagccc
    14761 gggatctcga tacgagcccc gctcttaccg aagacgctgt agtagccttc ttcagtcaac
    14821 tgtgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcaa ctggccttta
    14881 tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttagtcat gcaagaaccg
    14941 ataaatactt cgtcgatctt ctcgccctgt accgcagaca gcggacgcgc gtcatccggg
    15001 tcgttcggag cacacaggat tggctcttta atatccgcca gatcgatgtc aatcactgcc
    15061 gcgtattccg catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg
    15121 ccctgaatac gacgttccag ggtacgacga tcgccgtaac cttccgcgat catccacttc
    15181 agcaggacga tattagagtt aaggtattcg atgattggtt ctttgttcag cttgatagta
    15241 caaccagcgg cagaacgctc agcggacgca tcggtaagct caaaggcctg ctcaactttc
    15301 agatccggca gaccttcaat ttccagaatg cggccagaga agatgttttt cttgcctttc
    15361 ttctcaacgg tcagcagacc ttgtttgatg gcatacagcg gaatggcgtg taccagatcg
    15421 cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga ttccggcata
    15481 tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc cgccgggaaa
    15541 gagataccga tcgggaaacg ggtatgggag tcacctccgg taccgacggt atccggcagc
    15601 agcatacggt tcagccagga gtggattacg ccgtcacccg gacgcagcga cacaccgcca
    15661 cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac
    15721 gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg
    15781 tctttcagtt catcacgggt catcgggccg gtagtgtcct gtgaacctac agaagtcatt
    15841 ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc cacaggcacg gcctaccatt
    15901 ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca
    15961 tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg cccgataatc
    16021 agcgggatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag
    16081 gtcgccagca gttcgcctgt ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca
    16141 atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc acccgcgtct
    16201 tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt
    16261 ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt cgtggcggat
    16321 ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa acctttctgt
    16381 tgcagagctt caatctgttt gatcgggcca acaacacctg gctggtctgg ctcaatacct
    16441 tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg cgaccacgca
    16501 tccggtgccg gagaaaggtc atcggtgttg gtttcgccag tgactttgaa gacggtaacg
    16561 gtcagttttt cagccagcgc cgggcgattc ataaaccatt cggcatccgc ccaggactgc
    16621 attacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc atagaagtta
    16681 tcgaacatca gcagtgtgtg agaaagtgct ttggcagcga tcggtgccag tttggcatca
    16741 tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc cagcagttcg
    16801 atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttcgcgat agcagccagt
    16861 aagcctgctt tgacataggc ggcttcatcg acgccagggg gaacacggtt ggttaacaga
    16921 tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac aagtgcggcc
    16981 atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc
    17041 ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg
    17101 attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg
    17161 gcagaatagc aggattttga cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag
    17221 tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact tcgttaattc
    17281 gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactaccgc
    17341 ctctttatac tcggattaac agcacctgcg ggtgacagtt cgccgaccat tgcgatttcc
    17401 ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcactaattt
    17461 gcagtgccgg gctttgtgcc gcctctgccg ggctatatat aaaaagccgc acagtggaag
    17521 cggctgtgga aacgcaatcg acacaactgg ctgtgtctga cgctgccgcg gttacgcttc
    17581 ctgcaacggt ttccgcgcct cccgtaacac ccgccgtcgt taaatccgca ttcagcactg
    17641 cacaaataga tcaatgggtc gcgcccgtcg cgctgtatcc cgacgcccta ctttcgcagg
    17701 tgctgatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata
    17761 atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg tgggacgcca
    17821 gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa aacccgcaat
    17881 gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg gactcggtac
    17941 agcgattgcg gcaactggcg caacaaaccg gttcgctgaa gtcatcaaac gaacaaaaag
    18001 ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggca cccgtcatac
    18061 catccaatac cgttttaact gccagccccg tcattacaga gcctgcaaca accgtcattt
    18121 ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca accgtggttt
    18181 acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca gccggagaac
    18241 cgtttattga cagctttgtg cgcggattcg gctatagcat gggtgttgct accacgtacg
    18301 cactattcag cagcatcgac tgggatgacg acgatcatga ccatcatcat catgacgatg
    18361 atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa cacaacggcg
    18421 acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat cttactgata
    18481 agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat catgatcagg
    18541 atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc acgcagctac
    18601 ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag caacgaacac
    18661 acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag cggtttaatg
    18721 aagctgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc caacccctga
    18781 cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc agcttctcct gagcagcgcc
    18841 aggcagttcg cgagagaatg cagactaacc cgcagatcca gcagcgaaga gaggcagcgc
    18901 gtgagcgcat tcagcccgcc tcgcctgagc agcgccaggc agtccgcgag aaaatgcaga
    18961 ctaacccaca gatccagcag cgaagagacg cagcgcgtga gcgtattcag tcagcctcgc
    19021 ctgagcagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac caacagcaac
    19081 gtgataacgc ccgccagcgt gttcaatcag catcacctga acaacgtcag gtttttcggg
    19141 agagagttca ggagagccgc ccacaacgtc taaacgacag taaccgtact gccagattga
    19201 ataacgatca acggtcagca gtacgcgaac gtctctctga gcgcggagca aggcgactgg
    19261 aaaggtaaat tacagacgta aaaaaagcgg cgtggttagc cgctttttta attgccggat
    19321 gttccggcaa acgaaaaatt acttcttctt cgctttcggg ttcggcaggt cggtaatgct
    19381 accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt gcgcatggat
    19441 ggtcagcgcg atgtcttcag cgtcacaacc catttcgatt gccaggccga tttcacccag
    19501 cagctcgccg ccgttggtac cgacaatcgc accaccgatc acacggtgag attctttgtc
    19561 gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac cagaagcagc
    19621 ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt tctcagtcag
    19681 acctacccat gcaacttctg gttcggtata ggcgatagac gggataactt tcggatcgaa
    19741 gtagtgtttt ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg
    19801 tgccagcatc ggctgaccaa cgatatcgcc gatagcaaag atgtgcggta cgttggtacg
    19861 cagctgtttg tcaacgcgga tgaaaccacg gtcgtccact tccacgccag ctttgcctgc
    19921 gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt cgtaacgctg
    19981 cggttcagcg ggtgcttttt tgccttccat cgtcacataa ataccgtctt ctttcgcttc
    20041 aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac gcttggtgaa
    20101 gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt caaccacgtc
    20161 aatctgtgaa cccagcgcgt ggtatacggt gcccatttcc agaccgatga taccgccacc
    20221 cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg agtcccagat
    20281 acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg ctgcaatgat
    20341 cgcgttgtcg aagttgatca cggttttacc gttctcacct tcaacttcca gggtgttagc
    20401 cccggtaaat ttacccagac cgttgaccac tttgactttg cggcctttcg ccataccagc
    20461 cagaccaccg gtcagctgat tgatcacttt ctctttccag gtacgaatct tgtcaatatc
    20521 ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt cttcgataac
    20581 ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc aaacaccgcc
    20641 aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc aacggaaggc
    20701 agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga tttcagtact
    20761 catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc aggccgcagc
    20821 ggcgttagct tcacgccctc accccagtca cttacttatg taagcccctg gggattctgg
    20881 cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata ccctaaccac
    20941 caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt aaacaacaaa
    21001 cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt accagaaaaa
    21061 agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt cagacagcgt
    21121 gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa
    21181 ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc acaccggctc
    21241 catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa
    21301 gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct gcatttcgcc
    21361 cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc gagacagctc
    21421 gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt tcggggtatc
    21481 caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt cttccgacag
    21541 cgaactattg aagcgaggca tctgctcaag agctgcagca acggctttca tgatgaagac
    21601 aaccggggtg atcttcacat ccagcttacg tttcgccgct tcttcgttct gctgtttacg
    21661 gaacgcttcc agctcggtga tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac
    21721 ccagttacgg ctcaggttag caccagaaat tttctggata cggcccagtt ccacttcttc
    21781 gatttcacca aacttgctga agtccacctt cggccacggc agcatgcctg ggataccgcc
    21841 gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt aagcctgaac
    21901 gtcttcgcgc aggatacgac ctttacggcc agtgcccttc actttcgcca ggttaacacc
    21961 aaactcgcgt gccagacggc ggatcagcgg agtcgcgtga acataagcgt cgttttcagc
    22021 aaattcagat ttgccttccg ctttcgcagc tggtgctgct gccggggctt cagcttttgc
    22081 tgccggtgcc ggcgctgccg cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc
    22141 aacttcgaag atcataatca gcgagccagt tttcacttta tcgccaacgt tgactttcag
    22201 ttccttcacg acgcctgcaa acggcgccgg aacttccata gaagctttgt cgccttctac
    22261 ggtgatcagt gactgttcag cggcaacttt gtcgcccact ttcaccatca cttcagtcac
    22321 ttcaacttcg tcaccgccga tatccggaac gttaacttct ttcacgccag ctgctggtgc
    22381 aggggccgct gccggagccg cttcctgttt agccgccgga gctgccgcgc ctgcttcacc
    22441 cgcgacttcg aagaccataa tcagcgagcc ggtagacact ttgtcaccca cgttcacttt
    22501 gatctctttc acggtgccag caaacggagc cggaacttcc atagaagctt tatcgccttc
    22561 tacggtgatc agcgactgtt cagcttcaac tttatcgcca actttcacca ggatttcggt
    22621 cacttcaact tcgtcgctgc cgatatccgg aacgttaacg tcttttgccg ccgcagccgc
    22681 gggtgctgct gccggagctg cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc
    22741 tgcaccgtcg gcggaatcga aaatcataat cagtgcgccg gtctgggttt tatcgccaac
    22801 agagactttg atctctttaa cgatacccgc ctgcggagac ggaacttcca tagaggcttt
    22861 gtcgccttct acggtgatca gcgactgttc ggcttcaact ttgtcgccca ctttgaccag
    22921 gatctcggtg atttcaactt catcagcccc gatgtccggt actttgattt cgatagccat
    22981 tattctttta cctcttacgc cagacgcggg ttaactttat ctgcatcgat gttgaatttg
    23041 gcgattgcgt cagcaaccac tttcttatcg atttcgccac gtttagccag ttcgcccagc
    23101 gccgcaacca cgacataaga agcatcaact tcgaagtggt gacgcaggtt ctcacggctg
    23161 tcggaacgac cgaagccatc agtacccagt acgcggtagt cgtcagccgg tacgtaagta
    23221 cggacctgct cagcgaacag tttcatatag tcggtagatg ccactgccgg agcgtcgttc
    23281 atcacctgag cgatatacgg aacgcgcgga gtttccagcg gatgaagcat gttccagcgt
    23341 tcacaatcct gaccatcacg cgccagctcg gtgaaggagg tcacgctata aacgtcagaa
    23401 cctacgccgt aatctttcgc caggatctct gccgcttcac ggacgtgacg caggatagaa
    23461 ccggagccca gcagctgaac tttacctttg ctaccttcaa tggtttcgag tttgtagata
    23521 cctttacgga taccttcctc agcaccttcc ggcattgccg gcatgtggta gttttcgttc
    23581 agcgtggtga tgtagtagta aacgttctct tgtttttcac cgtacatacg ctccagaccg
    23641 tcatgcataa tgacagcaac ttcgtaagcg taagccgggt cgtaagagat acagttcgga
    23701 atagtcagcg actgaatgtg gctgtgacca tcttcatgct gcagaccttc tccgttcagg
    23761 gtggtacgac cggaagtacc gccgatcagg aagccacgcg cttgctggtc gccagccgcc
    23821 cagcacagat cgccaatacg ctggaagccg aacatcgagt aatagatgta gaacgggatc
    23881 atcggcagat tgttggtgct gtaagaggtc gccgctgcca gccaggaaca acctgcgccc
    23941 agctcgttga tcccttcctg cagaatctga cctttctcgt cttctttata gtaagcaacc
    24001 tgctcgcggt cctgcggggt gtactgctga ccgttcgggc tgtaaatacc aatctgacgg
    24061 aacagacctt ccataccgaa agtacgcgct tcgtcggcga tgatcggtac cagacgatct
    24121 ttgatcgact tgttcttcag cattacgttc agggcacgaa cgaaagcgat agtggtagag
    24181 atctctttgc tctgctcttc cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc
    24241 ttctcggtga agttcggctg acggcttggc agataaccgt gcagtttctg acgctgtgcg
    24301 tgcagatagg tatgctcttc agaaccttcc gggaaggtga tgtacggcag tttttcgata
    24361 tctgcatcag acaccggcac attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc
    24421 attttcttaa cctggtgcgc gatgttttta ccttcagccg cgtcgcccat gccgtaacct
    24481 ttaatggaat gagcaaggat tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat
    24541 gcagcgtaga ttttcttcgg atcgtgacca ccacggttca gtgcccagat ctgctcgtca
    24601 gtccagtctg caaccagtgc tgcggtttca ggatatttac cgaaaaagtg ttcacgaacg
    24661 tacgcaccat ctttcgattt gaaggtctgg tagtcgccgt caacggtttc gttcatcagc
    24721 tggatcagtt taccgctggt atctttacgc agcagttcat cccaacggct accccacatc
    24781 actttgatca cgttccagcc agcaccttcg aagatgcctt ccagttcgtt gatgatcttg
    24841 ccgttaccgg tgaccgggcc gtcaagacgc tgcaggttac agttgataac gaagaccagg
    24901 ttatccagtt tttcacgggt agcgatggtg atcgcacctt tggattccgg ttcgtccatt
    24961 tcaccgtcgc cgaggaacgc gtaaacggtc tgtttagagg tatctttcag gccacggtgt
    25021 tccagatatt tcaggaattt agcctggtaa atagcaccaa tcggccccag ccccatagat
    25081 acggtcggga actgccagaa ttccggcatc agtttcgggt gcggatagga agagaggcca
    25141 ttgccgtgaa cttcctgacg gaagttatcc agctgctcct gagtcagacg accttccagg
    25201 aaagcacgag cgtacacgcc cggggagatg tggccctgga agtaaaccag gtcgccgcca
    25261 tcctgctcgt tgcgtgcacg gaagaagtgg ttaaagcaca catcataaat ggttgcggaa
    25321 gactggaagg acgccatatg gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc
    25381 gtcatgatgg cgttccagcg gatagctgaa cgaatacggc gttccagttc cagattaccc
    25441 ggatactccg gttgttcttc aacggggatg gtgttgatgt agttgctgat acctgtgcct
    25501 gcggctacgt ttacaccgcc tttgcgggct tcagcaagca gttggtcgat cagatactga
    25561 gcacgctcaa caccttcttc acggatgacc gattcgatcg cctggagcca gtcgcgagtt
    25621 tcgatcggat ccacgtcatt tgggaaacgt tctgacatgg gttattcctt atctatctaa
    25681 taacgttgag ttttctggaa cctgtcccat tgaactctcg ccggaaagct caataagaca
    25741 ggttctacgt ttagttgccg cgcttttata tgcgcttgat ttacaacatc ttctggataa
    25801 tttttaccag taaaatcact aattctttcg ttgctccaga cgacgcagag aacgctcacg
    25861 gcggctctct tcacgacttc tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg
    25921 cgatgcttcg cgcgcttctt ccggcttacc ggccataatc gcttcaaata tgcgggtgcg
    25981 gtgactactc accagcggca gcatctcgcg acgcgaatag agcaattcga agttctggcg
    26041 gacattctgg gccaacatcg gctccataca ccttagcaga tgaagcagaa ccacattgtg
    26101 ggccgcttca gtgacagcaa tctgatactg gagtacggcg tttgattccg cgtccagatc
    26161 gccagactgc tgcgccagtt ctatggcgtg atggagttca cggatgcgtt ccttgtcttc
    26221 atcggtacta cgcagcgcgg cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc
    26281 gagcaagtca tactgtgact caggatggtc ggagagcagc tccaccagcg gatcgctgaa
    26341 gctttgccat aggctgctct ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc
    26401 cttcgcttcg agacgttgaa tcgcctcacg caaggaggga cgggagacgt caaactgttt
    26461 tgccagttcg cgttccggtg ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa
    26521 aaactccagt tgctgctcaa tcacatcgga gagttttggt tggcggattt tgctgtaggc
    26581 catgagttcc tgtcttaagc cacttgccga agtcaattgg tcttaccaat ttcatgtctg
    26641 tgacgctaaa gtaacaaagt attcacctta tgtccataca ggttttgatt gaaatcatga
    26701 aactgtgcac attttaacaa cttgacatat ataacgtttc aaagttgtaa ctatgcacaa
    26761 atgtagactt tacgtaggaa agggattttt taacgattat taaactataa aaatccgaat
    26821 tgaaccgatt cacttaccaa ttttgtgatt tttaattcaa ttaaaacgaa tttaaattca
    26881 ttctacatat tgagaggggt tgaggctgag ctttacaaac ggtttctttt taagcaactc
    26941 atcttcaacc atgcataaag cgggtgcatt cgctgccgca taccattatt cttgatctga
    27001 cggaagtctt tttgtaacaa ttcaaacttc tttgatgtaa acaaattaat acaacaaacg
    27061 gaattgcaaa cttacacacg catcactgcg tagatcaaaa aaacaaccac cgcacgaggt
    27121 ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc gcggccttaa aaaccgccat
    27181 attcagctta tcgcgctggg tggcgcgata gggaccgggt tattcctggg tagcgcctcc
    27241 gtaatacagt ccgcagggcc agggattatc ctgggttacg ccattgctgg ttttatcgcc
    27301 tttctgatca tgcgtcagct gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt
    27361 agccactttg cttataaata ctggggcagt tttgccggtt tcgcctctgg ctggaactac
    27421 tgggtactgt acgttttagt tgccatggct gagctgactg ccgtgggtaa atacattcag
    27481 ttctggtatc cggaaatccc cacctgggtt tctgccgccg tattctttgt ggtgattaac
    27541 gccatcaacc tgaccaacgt taaagtgttt ggcgagatgg agttctggtt tgccattatc
    27601 aaagttatcg ctgtggtagc gatgatcatc ttcggcggct ggctgctatt cagtggcaac
    27661 agcggcccgc aggcgaccgt tagcaacctg tgggatcagg gtggtttcct gccgcacggc
    27721 ttcaccgggc tggtgatgat gatggcgatt atcatgttct cgttcggtgg tctggaactg
    27781 gtggggatca ccgcagcaga agctgataac ccggagcaaa gtataccgaa agcaactaac
    27841 caggttatct accgcatcct gattttctat attggttcgt tagccgttct gctctcactg
    27901 atgccgtgga cccgcgttac cgccgatacc agtccgtttg tgctgatctt ccacgagtta
    27961 ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtac tgactgcggc gctctccgtg
    28021 tacaacagct gcgtatattg caacagccgt atgctgtttg gtctggcaca acagggtaat
    28081 gcgccaaaag cgctggcgtc tgtcgataaa cgtggtgtac cagtaaatac cattctggtg
    28141 tctgcactgg taacggcgtt gtgcgtactg attaactacc ttgccccaga gtccgctttc
    28201 ggactgttaa tggcgctggt ggtatctgca ctggtaatca actgggcgat gattagcctg
    28261 gcgcatatga aattccgtcg cgccaagcag gaacaaggcg tggtaactcg cttccctgct
    28321 ctgctttatc cgctgggtaa ctggatctgc ctgctgttta tggcggcggt actggtgatt
    28381 atgctgatga ccccaggaat ggcgatttcg gtatacctga tcccggtatg gctgatcgtg
    28441 ttaggtatcg gctatctgtt taaagagaaa accgccaaag ccgtaaaagc gcattaatct
    28501 ctctacgccc tcacccgtac agggtgaggg caataatctt tacaccaacg ccccgtaaat
    28561 cgtcagtagt gcaatcacca ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc
    28621 tgccttcggc gtctccacct tatcgacatg cggttcacgc gccagagaga actgcgccag
    28681 acgcgttaac acctgatact gcgaagtatg gaagtcaccc agcgaagcaa accaggccgg
    28741 taacgctttc tccccatgac cgatcaaggc atataccaca cccgcaagac gaaccggcac
    28801 ccaatccagt acatgaagca cggcatcaat gccggactgt aaacgatgat gcggcgtctg
    28861 gtatcgcgcc agccagtatt gccatgcacg caaaaacgca taccccatca gcgtaacggg
    28921 tccccagatt ccccccacaa tcaaccagaa cagcggagca agataaaaac gaaagttaat
    28981 ccacagcaat gcattttgca gctcacgcaa atactcacgt tcatcgcagc ctgccgggac
    29041 gccgtgaatc atggtgagtt cgccagccat cgtgacacgg gcatggctat cattacgtga
    29101 agcagctgtc agataagcat gataatgaag acgaacttta cctgcgccaa tacacagcaa
    29161 accaatcagc agccacacca gcagcgtggg aacgttgaac aatactccct gcaatgcgcg
    29221 taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga
    29281 aaaatgtttc acccgccgaa agaacgcttc aagacgatga tcaagctgcc agtgctcgcc
    29341 caacttaaac aggcgctcga aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc
    29401 tccttgctga ccagcgcacg aaaccgcgcc caatcaaatg cagggccggg atcggttttc
    29461 cgatccggcg caatatcaca atggcccgtc atgttattag cgatatctgg atagcgatca
    29521 atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg catcggtata cgccagcgta
    29581 tcggtgcctt caagctcaat cccaatagaa aaatcattgc agcgttcgcg cccctgatac
    29641 tgagagactc ccgcatgcca tgcacgttta tcgaaaggaa catactggac tatttcacca
    29701 tcacggcgaa tcaaacagtg agcggagacg cgcaaatggg cgatctcagc aaagaaagga
    29761 tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga tccacggacc gccaaactcg
    29821 cctggcggca ggctaatatt gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg
    29881 caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca gccacccctg ttctaacaac
    29941 atgcaggatc tccttatatg tggtgctaat acccggttca gagtagcatg tttctacctt
    30001 atgattcgtt agctatctgg agttttaaca tgccgcctcg ccgctataac cctgacaccc
    30061 gacgtgacga gctgctggaa cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc
    30121 tgcgggaaga tttaggcgga acagtcgatg ccaacaatga cattacggca aaacttttac
    30181 cggaaaactc tcgctctcat gccacggtga tcacccgcga gaatggcgta ttttgcggca
    30241 aacgctgggt tgaagaggtg tttattcaac tggcaggcga cgatgtcacc ataatctggc
    30301 atgtggatga cggcgatgtc atcaacgcca atcaaccctt gttcgaactt gaaggcccat
    30361 cccgcgtgct gttaacgggc gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag
    30421 ttgccagtaa ggtacgccac tatgtcgaat tactggaagg caccaacacg cagttgttgg
    30481 atacgcgcaa aaccttacct ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg
    30541 gcggagcgaa tcaccgtctg gggctttctg atgccttcct gatcaaagaa aatcatatta
    30601 ttgcctccgg ctcagtgcgc caggccgtcg aaaaagcgtc ctggctgcac ccggatgcgc
    30661 cagtagaagt cgaagtagag aatctggaag aacttgatga agccctgaaa gcaggagccg
    30721 atatcatcat gctggataac ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca
    30781 acggcaaggc gctactggaa gtgtctggca acgtcactga caaaacactg cgcgaatttg
    30841 ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac taaacacgta caagcactcg
    30901 acctttcaat gcgttttcgc taattgctga cagatttatc cgctccatcg cgggcggata
    30961 atctcccctt ccccaacttt cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac
    31021 gaaataaaaa acctcggaag ctgctttccc gtttcgtttt ttgcccctcg cttgtgctga
    31081 tggcgtttgc caaagtagcg ccaaccaaat caaggagcga aacagatgga caagcaacgc
    31141 ggttttacac ttatcgaact gatggtggtt attggcatca ttgccatttt aagcgccatt
    31201 ggtattcccg cttatcaaaa ctacctgcgc aaagctgcac tcaccgacat gctacaaacc
    31261 tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg aacatggtg
//
LOCUS       DAJTYD010000030        30900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTYD010000030 DAJTYD010000000
VERSION     DAJTYD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.030"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO585_001237"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO585_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6628891.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO585_001238"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO585_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6628892.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="OO585_001239"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="OO585_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6628893.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="OO585_001240"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="OO585_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6628894.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="OO585_001241"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="OO585_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6628895.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="OO585_001242"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="OO585_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6628896.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="OO585_001243"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="OO585_001243"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6628897.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="OO585_001244"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="OO585_001244"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6628898.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="OO585_001245"
     CDS             12677..13216
                     /locus_tag="OO585_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCR6628899.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO585_001246"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO585_001246"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6628900.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO585_001247"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO585_001247"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6628901.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO585_001248"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO585_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6628902.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO585_001249"
                     /pseudo
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO585_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO585_001250"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO585_001250"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6628903.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO585_001251"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO585_001251"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6628904.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO585_001252"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO585_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6628905.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="OO585_001253"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="OO585_001253"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6628906.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="OO585_001254"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="OO585_001254"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6628907.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="OO585_001255"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="OO585_001255"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6628908.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="OO585_001256"
     CDS             22396..22986
                     /locus_tag="OO585_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6628909.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="OO585_001257"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="OO585_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6628910.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="OO585_001258"
     CDS             23458..24153
                     /locus_tag="OO585_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6628911.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO585_001259"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO585_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6628912.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="OO585_001260"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="OO585_001260"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6628913.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="OO585_001261"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="OO585_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO585_001262"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO585_001262"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6628914.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO585_001263"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO585_001263"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6628915.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7325 a         7979 c         8420 g         7176 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 ttacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgtg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gtgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagctc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgaacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc aactgctctt taacaattta tcagacaatc
//
LOCUS       DAJTYD010000031        29903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTYD010000031 DAJTYD010000000
VERSION     DAJTYD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.031"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1974)
                     /locus_tag="OO585_001264"
     CDS             complement(<1..1974)
                     /locus_tag="OO585_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6628916.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLVIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRG"
     gene            2092..3285
                     /locus_tag="OO585_001265"
     CDS             2092..3285
                     /locus_tag="OO585_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6628917.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(3349..4602)
                     /gene="glyA"
                     /locus_tag="OO585_001266"
     CDS             complement(3349..4602)
                     /gene="glyA"
                     /locus_tag="OO585_001266"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6628918.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            4930..6120
                     /gene="hmpA"
                     /locus_tag="OO585_001267"
     CDS             4930..6120
                     /gene="hmpA"
                     /locus_tag="OO585_001267"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6628919.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(6165..6503)
                     /gene="glnB"
                     /locus_tag="OO585_001268"
     CDS             complement(6165..6503)
                     /gene="glnB"
                     /locus_tag="OO585_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6628920.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(6564..7898)
                     /gene="glrR"
                     /locus_tag="OO585_001269"
     CDS             complement(6564..7898)
                     /gene="glrR"
                     /locus_tag="OO585_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6628921.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(7888..8601)
                     /gene="qseG"
                     /locus_tag="OO585_001270"
     CDS             complement(7888..8601)
                     /gene="qseG"
                     /locus_tag="OO585_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6628922.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(8766..10193)
                     /gene="qseE"
                     /locus_tag="OO585_001271"
     CDS             complement(8766..10193)
                     /gene="qseE"
                     /locus_tag="OO585_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6628923.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(10751..14638)
                     /gene="purL"
                     /locus_tag="OO585_001272"
                     /gene_synonym="purI"
     CDS             complement(10751..14638)
                     /gene="purL"
                     /locus_tag="OO585_001272"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6628924.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            14899..16455
                     /gene="mltF"
                     /locus_tag="OO585_001273"
     CDS             14899..16455
                     /gene="mltF"
                     /locus_tag="OO585_001273"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6628925.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(16452..16988)
                     /gene="tadA"
                     /locus_tag="OO585_001274"
     CDS             complement(16452..16988)
                     /gene="tadA"
                     /locus_tag="OO585_001274"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6628926.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(17013..17648)
                     /gene="yfhb"
                     /locus_tag="OO585_001275"
     CDS             complement(17013..17648)
                     /gene="yfhb"
                     /locus_tag="OO585_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6628927.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            17857..18705
                     /locus_tag="OO585_001276"
     CDS             17857..18705
                     /locus_tag="OO585_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6628928.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            18761..19021
                     /gene="yfhL"
                     /locus_tag="OO585_001277"
     CDS             18761..19021
                     /gene="yfhL"
                     /locus_tag="OO585_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6628929.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(19215..19295)
                     /gene="shoB"
                     /locus_tag="OO585_001278"
     CDS             complement(19215..19295)
                     /gene="shoB"
                     /locus_tag="OO585_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6628930.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(19715..20095)
                     /locus_tag="OO585_001279"
     CDS             complement(19715..20095)
                     /locus_tag="OO585_001279"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6628931.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(20095..20826)
                     /gene="pdxJ"
                     /locus_tag="OO585_001280"
     CDS             complement(20095..20826)
                     /gene="pdxJ"
                     /locus_tag="OO585_001280"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6628932.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(20838..21566)
                     /gene="recO"
                     /locus_tag="OO585_001281"
     CDS             complement(20838..21566)
                     /gene="recO"
                     /locus_tag="OO585_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6628933.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(21578..22483)
                     /gene="era"
                     /locus_tag="OO585_001282"
     CDS             complement(21578..22483)
                     /gene="era"
                     /locus_tag="OO585_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6628934.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(22480..23160)
                     /gene="rnc"
                     /locus_tag="OO585_001283"
     CDS             complement(22480..23160)
                     /gene="rnc"
                     /locus_tag="OO585_001283"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6628935.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(23433..24407)
                     /gene="lepB"
                     /locus_tag="OO585_001284"
     CDS             complement(23433..24407)
                     /gene="lepB"
                     /locus_tag="OO585_001284"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6628936.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(24423..26222)
                     /gene="lepA"
                     /locus_tag="OO585_001285"
     CDS             complement(24423..26222)
                     /gene="lepA"
                     /locus_tag="OO585_001285"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6628937.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(26420..26899)
                     /gene="rseC"
                     /locus_tag="OO585_001286"
     CDS             complement(26420..26899)
                     /gene="rseC"
                     /locus_tag="OO585_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6628938.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(26896..27852)
                     /gene="rseB"
                     /locus_tag="OO585_001287"
     CDS             complement(26896..27852)
                     /gene="rseB"
                     /locus_tag="OO585_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6628939.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(27852..28502)
                     /gene="rseA"
                     /locus_tag="OO585_001288"
     CDS             complement(27852..28502)
                     /gene="rseA"
                     /locus_tag="OO585_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6628940.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(28535..29110)
                     /gene="rpoE"
                     /locus_tag="OO585_001289"
     CDS             complement(28535..29110)
                     /gene="rpoE"
                     /locus_tag="OO585_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6628941.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(29107..29262)
                     /gene="rseD"
                     /locus_tag="OO585_001290"
     CDS             complement(29107..29262)
                     /gene="rseD"
                     /locus_tag="OO585_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6628942.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            29518..>29903
                     /locus_tag="OO585_001291"
     CDS             29518..>29903
                     /locus_tag="OO585_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6628943.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRR"
BASE COUNT         7171 a         7986 c         7543 g         7203 t
ORIGIN      
        1 accgcgccac tgcgcgagag ctgattcgct acgaccatcg aaccagttca gccaccacag
       61 agtggcgttc agcggataat cgcgcagcag tttctcgcgc tgcacacgcg cgttgtcctg
      121 acgaccactt aacaccagca gcagattatg caggcaaagc acttcctgat tggttgggca
      181 ggcgcgaaga ctttgttggc aaaaatccag accagcgtcg aagttaccat tacgcgccgc
      241 cagtcgtgcc aggccataat agccaccggc tttgctgttg ccgctccaga ccgcacgcca
      301 gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg cgcgaatcaa
      361 actcgcctgt ccgcactgcg gatttttgtt cagcgcatgt gcgcgtttca gagcctgagt
      421 ggcatacgcc accgcttgcg ggaaatcggc gcggttatat tccagcatcg ccagcgccag
      481 gttacagcgg taatccagcg gatccagcgc cacgccgcgc aggtagtaat cgaacggtga
      541 acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt catctgtact
      601 ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc agcaacggca actcttgcgg
      661 ctgatgttcc tgataactca gtacaatatt gccgtcggca tcggagagct caatggtcag
      721 cctttcagga ttgataccgt gcaacacgcc ctggatggcg gtcgcaggca tcagtgccac
      781 ggcatcatca agtaacgcgt tgcatttgcc gatttcgcgg atcaccaggc ggtatccgtt
      841 caacggagag atggcataca gcccccactc aatcccccgc ttactacgct ggagttttat
      901 caccgcatcg cgggaggcat tttgcaccat gcccaaagaa tgataaggca ggaaatactg
      961 ctcaaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcgggctggt tatcggcaaa
     1021 aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct tatcccacgc
     1081 ctggccaaat tcactgtgtc cccaactcca ctgtttttta cctggcgcaa tatggtggtt
     1141 ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac gcgccgacaa aatcgtactg
     1201 cgatttttcg gccatatatg aggttgtaac aggcacattt ttatagcaag aaatgtccac
     1261 tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag cggagacggc
     1321 ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg aaaacgctct gatgcccttc
     1381 cccccctttc actgccgggt tggcccacca caagaaatga cgcggcgtgg cgttaccgtt
     1441 atagacgcgg ctggcgattt ccagcgccgc ccggtcaggg cgcagggtga aacctgtcat
     1501 cacctgtaaa ccatgcatcg gctccgtttc gcctacccac accgtctgtg caccgtcttc
     1561 atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatgat gttgcggcca
     1621 gttaaactca atcccaccgg aaatccacgg ccccagcagc cccaccagcg caggtttaat
     1681 gacttcattg tgataaacaa aatcgcgctg tttcacttta tcccacgcgc gatgcacccg
     1741 accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc acaccgcctg
     1801 ccaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg gataaaccgc
     1861 gccggacgat ccctgataaa cgcgattttc caggaacatg ggatgtatat cctgcggccc
     1921 ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag tcatggtgcc
     1981 ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg aacaatctgg
     2041 caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa gatgagagcc
     2101 tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga actgctgtac
     2161 cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc gattccggca
     2221 gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat tgacgatgaa
     2281 agccagacgc gcgggcatag tagcggtaca tggctgattg cgccggaagg tgactggacg
     2341 ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc ttgtttaagt
     2401 ccgaaaggtg aatttgagta tttacagatt gatgcaccga cgccgcaggc gctgctgtcc
     2461 gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac catcaatctg
     2521 gcgctggcaa tccacggtca ggttgatccg gtgaccggcg tgtcgcaaac catgccgcaa
     2581 gcgccgtgga caacgccggt tgaggtgaag tatctgctgg aagagaagct gggcattcgg
     2641 gtgatggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca aaataattcg
     2701 caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc gttcgtgatt
     2761 aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac aaattggtca caccatcgtt
     2821 aatccggatg gcgtcgtctg cgactgtgga cgttacggct gcctggaaac cgtcgcctcg
     2881 ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt taatactcag
     2941 cttgatcctg aaaaactgac aaccgcgcag ttaatcgctg cctggcagag tggagaaccg
     3001 tggatcacca gctgggttga tcgctctgcc aatgccattg gtttgagtct gtataacttc
     3061 ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc ctttggtgag
     3121 aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg cgacgaagga
     3181 ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca acaggtgctg
     3241 ggaattggct atttgtatgt tgaggcgcag ttacgacaga tttgatggcg cgataacgta
     3301 gaaaggcttc ccgaaggaag ccttttttca acagattaac cgtttcgctt atgcgtaaac
     3361 cgggtaacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa cggcttcatc
     3421 attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt ctgcttcttt
     3481 gaagccgcga cgcgtaattg ccggagtacc cacgcgaata ccggaggtca caaacgggct
     3541 cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc ccagagcggc
     3601 gtctgcttct ttaccggtca ggtttttatc aaccagatca accaggaaca ggtggttatc
     3661 agtgccgccg gaaaccactt tgtagccgcg ctcgaggaac acttctacca tcgctttagc
     3721 gtttttcgcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt ctttcagagc
     3781 aaccgcttta ccggcgatta cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc
     3841 agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca ggccgccgcg
     3901 cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag gaaccgggtt
     3961 cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga acaggtaagc
     4021 accgatgctg tcagcgattt cacgcatttt cgcccagtcc accacgccgg aatatgcaga
     4081 gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca gatcggcgta
     4141 gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt taccggagaa
     4201 gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac ccagaacggt
     4261 atcacctggt tccagcagcg cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg
     4321 ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg ccagttgttc
     4381 aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac cttcagcata
     4441 tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt agttttcgga
     4501 ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag cctgccacag
     4561 ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct cctgactcag
     4621 ctaacaataa aatttttggc ctttataggc ggtcctgttg gacaacggcg aacagtataa
     4681 ccgaatcatt gtgcgataac aggtcttgac aaaggaattt acgcaaacga ttaccttcag
     4741 gctacgcaag gctttggaga ataaagagct tgcaaccgga aacggatttc ttttcaggtt
     4801 tgtgatgcaa atttttcact tcatcacatt ctttctgaaa aacaccaaag aaccatttac
     4861 attgcagggc tattttttat aagatgcatt tgaaatacat caattaagat gcaaaaaaag
     4921 gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg
     4981 gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca
     5041 gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg
     5101 tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg
     5161 gtagaaaaaa tcgcgcagaa gcacaccagc ttccagatca aaccggaaca gtacaacatc
     5221 gtcggtgaac acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg
     5281 gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa
     5341 atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt
     5401 gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt
     5461 ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa gccggaaggt
     5521 ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat
     5581 cgtattgcgg tgaaacgcga agagggtggg caggtatcca actggttgca caatcacgcc
     5641 aatgttggcg atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca
     5701 gatgacacac cagtgacgtt aatctcttcc ggtgttggtc aaacgccaat gctggcaatg
     5761 ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa
     5821 aatggcgatg ttcacgcctt tgccgatgaa gttaaggaac tggggcagtc actgccgcgc
     5881 tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt
     5941 gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg
     6001 cagttctatc tctgcggccc ggttggcttc atgcagttta ccgcgaaaca gttagtggat
     6061 ctgggcgtga agcaggaaaa catttattac gaatgctttg gcccgcataa ggtgctgtaa
     6121 tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg
     6181 tcctcttcac cggtacggat gcgaatgacc cgtgccacgt caaagacgaa gattttaccg
     6241 tcaccgattt tgccggtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg
     6301 tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg
     6361 ccgcggtaca gctcggtatg gcctttctgg cgaccaaagc ctttcacttc ggtcaccgtc
     6421 atgccggtaa taccgacttc ggccagtgct tcgcggacat cgtccagctt gaagggtttt
     6481 ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct tttgatctgc
     6541 taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg
     6601 gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg
     6661 ttgcctttgg taatttgcag cagcttacgc aaatagttga gttcaaactg attacgtgcc
     6721 tcaacaaagg ttggcagcgc cgtgttttca ccctccagcg cctgctctac taatgcatcg
     6781 ctaataaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgc
     6841 acattacccg gccagctagc ggtcatcagg cgtttcatcg catcagtgga gaacgcgcgg
     6901 acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag aagcggaatg
     6961 tcttctgtgc gctctgccag agccggaatt ttcaggctga caacgttgag gcggtaatag
     7021 agatcttcgc ggaattcccc gcgcgccatc gcttttggca gatcacggtg agtggcagaa
     7081 ataatccgca cattgatatc aatatcgcgg ttactgccca gcgggcgcac tttacgctcc
     7141 tgcaacacgc gcagcagttt gacctgtaac ggcgcaggca tatcgccaat ttcatcgaga
     7201 aatagcgttc cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta
     7261 aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg caatgcgcca
     7321 cagttaatag caataaatgg tttgctgttg cgcgggctgg cgttgtggat agcctgggcg
     7381 aaaatctctt tcccggtgcc gctctgaccg ttaatcaaaa cgctgacgtc tgattgcgcc
     7441 accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac aattgcctcg
     7501 cgccagcgtt catcggtggc tggcgcggat tgctccagcg catcgtcaat tgcctgatat
     7561 agcgcgtctt tgtcgacagg cttggtgagg aaactaaaaa cgccctgctg tgttgcagca
     7621 acggcatcgg ggatagaacc atgcgcagta agaataatta ctggcattcc cggctgcact
     7681 ttctggattt cagcaaatag ctgcataccg tccatttcat ccatccgcag gtcgctgata
     7741 actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact ttccgccgtg
     7801 accacactgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg
     7861 tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga
     7921 tggcgcgggc ttttcactct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg
     7981 ggtcgagagc tggcgttcaa tatcggtcag gttttccagc ttgcgggtgg tgagttccag
     8041 ttgctgctgt agaacgtgat gttgctggcg caatgtatcc agctcgctgt cgctggactg
     8101 ctggagttta ctgtaacgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc
     8161 gcgccagagt tgatacagtg ggcgaacctg tgccgggatc tcggtactta acgcttcaat
     8221 acgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc
     8281 ttgtttaaag gtattttgcc agcagccgtc gtcatattgg cgagcttgct gacgcgactg
     8341 cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc
     8401 ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag
     8461 ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgattctg
     8521 cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa
     8581 tcgttgaaaa atgtgtcgca tattcaccag acttaaagcc tatcccagtg ggcgtaattg
     8641 ttgcagacag tctggacatg gacagcgcgg agaaaccgga gcgtacatat cgtacgtgaa
     8701 gatttcgagc actgcccgga gccgaactga caaataaaat agcctgatag gataggctgt
     8761 tagcattatt tcgtgttttt cgacgacggt aattcaatgc ggaaacaaac gtcttgcccg
     8821 ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct ggcaatgctt
     8881 aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccctg aaaaaagggt
     8941 tcgaagatca tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat gacatcaata
     9001 taaacccgcg caccatgtga actgctgcga aggcaaatgt taccggattc agccccgtag
     9061 tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg ctccgccagg
     9121 caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc gggcaggcta
     9181 tgagcagaaa ccactgtctc caccagcggt gctaattcaa cattctccag ttccaccgca
     9241 ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg caaattgcgg
     9301 ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg cccgacaacc
     9361 tggtcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt taattcatga
     9421 gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg ctcacttaac
     9481 caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cactgaacga aacgctattg
     9541 cccagagaac gcccttcccc cagacggttg atcatgcgct cgatattttt caccggcccg
     9601 ataatcatcc gcgtgaaaag tagcaccatc accagactca ccagaaatag caccagcgat
     9661 tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg cccacgagag
     9721 aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa ggcttcaaga
     9781 cgcgcggcgg cggcagcatc gggaccactg ttgttacact gaagttgggc cagattgttc
     9841 aaatcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc gtgggcatcg
     9901 agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt agggtcgtcc
     9961 agcacgcaat actgacggta gctacgctcc atctccagcg ccgcgttggt catcgcttca
    10021 ctacgccggg catcaataag cgtagtgcgg ttaaccagcg ccgcctgatc gctaagcgca
    10081 ttcaggcttt gccaggcttg ccatgccagc accaacaggg gtagtagaat tagcaaaaat
    10141 gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt caaggtgtta
    10201 ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata caacaaagcc
    10261 gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt tcgcccgatg
    10321 gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg tgcgtcattc
    10381 gaattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag ccactgctta
    10441 ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg attatacgtt
    10501 gctttgaggg atttatggct tcttcgctat ttgaatgtaa atcagccact gtgtcgctaa
    10561 aaagagacag cttaagataa acttattaga ttacaataaa atcaatcact taagtgtcgc
    10621 cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa aagctcccgg
    10681 agttgggagc ttatgatagt ggttggtgct taatgccgca tccgggctgc aaaaccaatg
    10741 aactgatgaa ttaccccaac tgcttacgcg cattgcggaa aatgcgcatc catgggccat
    10801 cctcgcccca gttttccgga tgccaggagt tgctgacagt acggaaaaca cgttccgggt
    10861 gcggcatcat aatggtgact cgaccacttt cagtcgtgac tgccgtaata ccgttcggtg
    10921 aaccgttcgg gttggccggg taggtttcag tgactttgcc gaagttatcg acatagcgca
    10981 gtgccaccag ccctttgctt tccagtgccg ccagatgcgc cgcatcacgc acttccacgc
    11041 gcccttcacc atgagagaca gcaatcggca tttgcgagcc caccatcccc tgcaacaaca
    11101 gagacgggct ttgggttact tcaaccaggc tgaaacgcgc ttcaaagcga tcggaggtat
    11161 tgcgcacaaa acgtggccac aactcactac ctgggatcag ttcgcgcagg ttagacatca
    11221 tctggcaacc gttacatacc cccagcgcca gcgtttgcgg acggtggaag aaggttgcaa
    11281 actcatcgcg tacacggtca ttgaacagga ttgacttcgc ccaaccttca ccggcaccca
    11341 gcacatcacc gtaggagaaa ccaccgcacg cgaccagggc gtggaaatct tccaggcccg
    11401 tgcgtccggt cagcaggtca ctcatatgca cgtcgatagc atcaaagcct gcacggtgga
    11461 aagctgccgc catttcaaca tgcgagttca cgccctgctc acgcagtacg gcaactttcg
    11521 gacgtgcgcc agtggcaata tacggtgctg ccacatcttc gttgatatcg aacgacagtt
    11581 ttacattcag gcccggatcg gcgtcgttag atttcgcctg atgctcctga tcggcacact
    11641 ccgggttgtc acgcaggcgc tgcatctgcc aggtagtttc tgcccaccag acacgcaacg
    11701 tggtgcggct ttcgctgaat acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg
    11761 aaaccgcctg ccctacataa tggacacaat cagcaagccc atgctgtgcc agtacggact
    11821 cgaccgcttc acggtcagcg gcacgaacct gaatcaccgc acccagttct tcgttaaaca
    11881 acgccgccag gcgatcgtcg cccagagagg caatatccgc gttaatgccg caatgaccag
    11941 caaaggccat ttccgccagc gttaccagca ggccgccatc agagcggtcg tggtatgcga
    12001 gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc agttgcgcga
    12061 cgtcgcgtac atctgccggt ttgtcgccaa gctgacgata aacctgcgcc agcgccgttg
    12121 cgcccagcgc gttattgcct ttgcccaaat caatcagcaa tagtgcgtta tcttcggtag
    12181 aaagctgtgg cgtgatggtg tgacgcacat cttccacgcg ggcaaatgca gaaatcacca
    12241 gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg
    12301 acatagagtc tttacccacc gggatcgtca ggcccagcgc cggacaaagc tcttcgccca
    12361 cggctttaac ggcttcatac aggcccgcat cttcaccagg gtggcctgcc gccgccatcc
    12421 agttggcgga aagtttaatg cgtttgatat cgccaatctg tgttgcggca atattggtta
    12481 acgcttcacc gaccgccaga cgggcagagg cggcgaaatc cagcagcgca accggcgcac
    12541 gctcgccaat cgccatcgct tcaccgtagt agctgtcgag gctggcggta gtgaccgcgc
    12601 agttagcgac cggcacctgc cacggaccca ccatctgatc gcgcgctacc atgccagtta
    12661 cgctgcggtc accgatggtc accaggaaag ttttctccgc cacggtcggc agatgcagta
    12721 tgcgtttcac cgcgtctgca atggtgatcc cttcacggac cagcgcatcg cccttcgctt
    12781 tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc aagcaggacg tccagcggca
    12841 gatcgatcgg ctgattatca aaatgacgat cgtgtagaga aagatgcagt tcttcggtcg
    12901 cttcaccaat caccgcgtag ggtgcgcgct cacgcttaca cagttcgtca aacagcggta
    12961 attgatcggc agcaaccgcc agcacgtagc gttcctggga ttcgttacac cagatttcca
    13021 gcgggctcat gcctggctcg tcgctcagaa tgtcgcgcag ttcaaattta ccgccacgcc
    13081 cgccgtcgct caccagttcc ggcatagcgt tagaaagacc gcctgcgcca acgtcgtgga
    13141 taaacagtat tgggttggca tcaccaagct gccagcaacg gtcaatcact tcctggcagc
    13201 gacgctccat ctccgggttg tcgcgctgta cggaagcaaa gtcgaggtcg gcatcagact
    13261 gaccagacgc catggaagac gccgcgccgc cgccaagacc gatgttcatt gccgggccgc
    13321 cgagaacgac cagcttcgcg ccaacgttga tctcgccttt ttgtacgtga tcggcgcgaa
    13381 tgttgccgat cccgcccgcc agcatgatcg gtttgtgata accgcgcagc tcttcgccgt
    13441 tgtggctgtt cactttttct tcataagtac ggaagtagcc gttcagtgcc ggacgaccaa
    13501 attcgttgtt aaacgccgca ccgcccagcg ggccttcggt catgatgtcc agcgcggtga
    13561 caatgcgctc aggcttaccg aaatcttctt cccacggctg ttcgaagcca agaattcgca
    13621 ggttggaaac ggagaaacca accaaacccg ctttcggctt cgcgccgcgc ccggtggcac
    13681 cttcatcgcg gatttcaccg ccggaaccgg tcgccgcgcc cggccacgga gaaatcgccg
    13741 tcgggtggtt gtgagtttcg actttcatca gaatatgcgc cggttcctga tggaaatcat
    13801 agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc agaaccttcc attacggcgg
    13861 cgttatcttt ataagcagag agaacgtgat ctggcgtggt ttcgaaagta tttttgatca
    13921 tcctgaacag cgatttcggc tgttgttcac cgtcgataat ccagtcggcg ttaaaaattt
    13981 tgtggcggca gtgctcagag ttcgcctgag cgaacatata cagctcgatg tcgttcgggt
    14041 tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat ttcatcttcc gccagagcca
    14101 ggccaagacg caggttagcg tcgatcagcg cctgacggcc ctgccccagc aaatcaacgc
    14161 ttgtgaccgg agtcggttga tggtgggcaa acaactgctc tgcatcatct aaatcaaaaa
    14221 agacagtttc catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg
    14281 tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc aaggcggttt acctgttgta
    14341 gcccgcagtt atgggcaata tcggtcgctt tcgaagacca gggagagatg gtgccaggac
    14401 gcggggtcac cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc ggaccatatt
    14461 tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa
    14521 aatggacata ctcggcgtaa atattgtgaa ccgggagcct ggcagcctga aaacgtgcca
    14581 gcagtttgtt gattcggaat gccgacagtg caggcgaacc acgcagaatt tccatcataa
    14641 gtctctcgtc ttctaagctt tcggtgtacc caaggggggg gggaaacggg cgtcattata
    14701 aagaatctga tgcgctgacg aaaccgtttg cgtggaaata aaatcaccat cgtgaattag
    14761 caacgcgtgc cgccaatggc tgtaataagt tgccatctgg cgcaggttta cgcaaaatgc
    14821 cgctcattta tgagtaaacc tttcactatt attacgtttt ttcaagctgg gacgcgcacg
    14881 acacagagaa ttaactaatt gaaaaaatta aagattaatt atctgttcat cggcattctg
    14941 gcactgctgc tcgcggtcgc tctctggcca tccattccct ggtttggtaa agccgacaac
    15001 cgtatcgccg ccattcaagc gcggggagag ttgcgtgtga gcaccattca tactcccctg
    15061 acttataacg aaatcaacgg gaaacctttt ggcctggatt acgaactggc gaaacagttt
    15121 gccgattacc tcggcgtaaa actgaaagtg accgtgcggc agaatatcag ccagctgttt
    15181 gacgaccttg ataatggtaa cgccgacctg ctggcggcag gactggtcta taacagtgag
    15241 cgggtaaaaa attatcagcc tggccctacc tattattccg tgtcacaaca actggtttat
    15301 aaagtgggtc agtatcgccc acgtacgctg tgcaacctga cggcggagca actcaccgtt
    15361 gcaccgggtc atgtggtggt taacgatctc cagaccctga aagaaacaaa attcccggaa
    15421 ttaagctgga aggtagacga caaaaaaggc tctgcggaat taatggaaga tgtcatcgaa
    15481 ggaaaactcg attacaccat tgctgattct gtcgccatca gcctgtttca gcgcgttcac
    15541 ccggagctcg ccgtagcgct cgatatcacc gatgaacaac cggtgacttg gtttagcccg
    15601 ttagatggcg ataataccct ttccgccgcc ctgctcgact tcttcaacga aatgaatgaa
    15661 gacggtacgc tggcacgcat tgaagagaaa tacctggggc atggcgatga ttttgattac
    15721 gtcgatacgc gcacattttt acgcgccgtc gatgcggtac tgccgcagtt aaagcccctg
    15781 tttgagaaat acgccgaaga aattgactgg cgtttgctgg ccgctattgc ttatcaggaa
    15841 tcgcactggg atgcacaggc cacttcaccg acgggtgtgc gcggcatgat gatgttaacc
    15901 aaaaataccg cgcaaagcct cggcattacg gatcgtaccg atgccgaaca gagcatcagc
    15961 ggtggcgtgc gttatttgca ggatatgatg agtaaagtgc cggaaagtgt gccggagaac
    16021 gagcggatct ggtttgccct cgctgcgtac aatatgggct atgcgcatat gctggatgcc
    16081 cgcgccctga cggcaaaaac caaagggaat cctgacagtt gggctgacgt aaaacagcgt
    16141 ctgcctttac ttagccagaa accctattac agcaagctga cttacggcta tgctcgtgga
    16201 catgaagcct acgcttatgt cgaaaatatt cgtaagtatc aaattagcct ggtgggttat
    16261 ctgcaagaga aagagaagca ggctacagaa gcggcgatgc aactggcgca ggattatccg
    16321 gcggtatcgc ctacggagtt gggcaaagag aaatttcctt ttctctcgtt tctttcccag
    16381 tcgtcatcaa actatttgac ccattctccc tctctgctgt tttccaggaa agggagtgaa
    16441 gagaaacaaa attaatccgt cgaggattgc gcttttttct gcgctttaat ttcctggcgg
    16501 cgcatgcgaa agaagtcact gagcaacgcc gcgcactcat ccgccagtat tccttccgta
    16561 atttccactc ggtgattcat acccggatga tgcagcacat ccattaaaga tcccgcagcg
    16621 ccagttttcg cgtcacgcgc accaaagacc acgcgaccaa tgcgactgtg gatcatcgct
    16681 ccggcacaca ttacacatgg ttcaagcgtg acatacaacg tggcgtcgat cagacgataa
    16741 ttttgcatca ccagaccacc ctgccgcagg gccatgattt ctgcatgtgc ggtgggatca
    16801 tggcgaccaa tcgggcggtt ccaaccttcg ccgattaccc gattgttatg cactaatacc
    16861 gcgccgaccg gcacttcccg ctcatcccag gcacgtttcg ccagcgtcag cgcgtgacgc
    16921 atccagtatt cgtggctaaa ttcgacttca gacaaaaaga aaactccggt tataaaagcg
    16981 cggcgcatta tacacggacg ctatgcttta ctctattcca gttgctggag ttcaccgcgc
    17041 ggggttactc gccaacgatg ctgacagaaa taaagcagcg gattgtcctg attactgtcg
    17101 ctatagccac tgtacagccg cagcggagtg ccgattttgc gctccagttg tgcgaccttt
    17161 tcatgtccca gacaacgcat cgtcaatacc caaccaccat agccacgctg aatttggctg
    17221 gcgataagat taacccgcgg cagccagggc ttatcgaaat aaaccgcttc aaccagcggc
    17281 tgcggagagc cggtaatcaa ccagatatca gcatcggaac ttaacaggta ggtggttaat
    17341 cgctcctgaa ccagcggaaa ggcggtaaca ttgtcgcgaa accagcgcac gaaatcggcc
    17401 tgcaacgtct gtaaacgtgc ttcgctgtga ccaaaagtgc acccccacag aagcagactc
    17461 atcggccagc gtgccgcacg accttttatc aataacgcaa tggctataat cggtaacaac
    17521 ggcaggacaa gtaacgcatt cagcggttgg cgacgtagta aatagcgcag aaaactgccg
    17581 aacatatcct gctgatgcaa tgttccatct aagtcaaaaa acaccacacg acgctcgtga
    17641 gttgccaaac catactcctc tggactgaaa catcctgatt aattttcgct actacatagc
    17701 ctaacagata gatcatcact tttccggcaa caatcgaccg catcatggct aactggaatt
    17761 tttaattcat ggcaattagc ggcaatggaa tataaaattc actcgcgtgt gtctcatatt
    17821 gcctgttgtc gccactattt catcgcgagg aaagatatga acggcttact tcgtatccgt
    17881 cagcgttacc aggggcttgc ccagagtgat aaaaaactgg cggattatct gctgctacaa
    17941 cctgatacgg cgcgccattt aagctctcag caactggcca acgaagccgg agtcagtcag
    18001 tccagcgtcg tgaagttcgc gcaaaaactc ggctataaag gttttccggc gcttaagttg
    18061 gcgttgagtg aagcgctggc aagccagccg gaatcgccct ccgtgcccat tcataaccaa
    18121 atccgcggtg atgatccgtt acggctggtc ggcgaaaaac tgattaaaga aaatactgcc
    18181 gctatgtacg caacgctaaa cgttaatagt gaagagaaac tgcatgaatg cgtaacaatg
    18241 ttgcgctctg cgcggcggat aattctgacc ggtattggcg cttcgggtct ggtggcgcaa
    18301 aactttgcct ggaagctgat gaagattggc ttcaatgctg ccgcagtgcg cgatatgcat
    18361 gcgctactcg caacagtaca ggcgtcgtcc cctgacgatc tgttattagc catttcctac
    18421 accggtgtac gacgcgagtt aaacctggcg gcagatgaga tgctgcgagt gggcggaaaa
    18481 gtgctggcga ttaccggctt tactccgaat gccctgcaac agcgtgcttc tcattgcctg
    18541 tataccattg ccgaagaaca agcgacaaac agtgcttcaa tctctgcttg tcacgctcag
    18601 ggaatgttaa cggatttgct gttcattgcg ctgattcagc aggatctgga actggcacca
    18661 gaacgtattc gtcatagtga agcgctggtg aaaaaactgg tctgagtaaa gaatgcgcgt
    18721 ataatgcccg cccggtttgt gttgttttga gagtttcctt atggcgttgt taatcactaa
    18781 aaaatgcatc aattgtgata tgtgtgaacc cgaatgcccg aatgaggcga tttcaatggg
    18841 agatcatatc tacgagatta acagcgataa gtgtaccgaa tgcgtagggc actacgagac
    18901 accaacctgc cagaaggtgt gcccgatccc caatactatt gtgaaagatc cggcgcatgt
    18961 cgagacagaa gaacagttgt gggataaatt tgtgctgatg caccacgcgg ataaaattta
    19021 atattctact ctggaagtag agtattaatt atattactgg gaagccttaa cgccattata
    19081 tttatttaat tgatgacatt agcataatca ttcactaagt taatttatat agtatctgcc
    19141 aagacactta tttatagtta ttaaaggcgc gtccgattgg ttcaccggac gcaccttaag
    19201 tacgtttcct tgtgttataa gaacagaagg atcagctgta aaacagcaat gatgattttg
    19261 atgacccgtt taatcaggta tcggcaatca gtcattcgtt tttccttaaa caaggaatgc
    19321 agccgttcag attagccctt acatctcccc aaaactgaac gtgcgagtta ttgagggtgc
    19381 atgctgcact ccacaccaga gctttgacga caccactcgt ttcaatgggg gaattctgtg
    19441 gcatggtgta aagcacagca aaatcttcaa taacgaagcc aattttaatg tacttacgaa
    19501 ttggcggtca cgttaatctt tccatcaata ttgctttctt cgtaaaggct cgagttttta
    19561 tgctaaagat tgcaagttgc ttgtaaaaga taagtacact gatccataat cgctgttgtt
    19621 gagggtgcat gctgcacaaa attaaagtta aaaagtaaaa cccccgttcc ttaccagttc
    19681 gggggtttta ctttttaaag agaacggtat tattttaact ttcaataatt accgtggcac
    19741 aagcatagtg gcgttcatct gccagcgtta catgcatatt tacaacgccg agcttttccg
    19801 ccagttttaa tgcctcaccc catagccgta gccgtggttt gccgagctca tcattgaata
    19861 cttcaaattg attaaacgcc agaccattgc ggatcccggt gccaaacgct tttgctgcgg
    19921 cttctttcac agcaaaacgc ttcgccagaa aacgcaccgg ctggtggtgc gttttccaga
    19981 ttgcccattc gttatcgctt aatacgcggc gtgctaggcg ctcaccggat cgggcgatca
    20041 ctgcttctat gcgagcgatc tctacgatat ccgtgcctaa acctaatatt gccattagcc
    20101 acgcgcttcc agcatcagac gcttcatttc tgccaccgca tctttcagtc cggtcatcac
    20161 tgcacggcca ataatggcat gaccgatatt cagttcatgc atctcaggga tggcggcaat
    20221 ggctttcacg ttgtgatagg tcagaccgtg tccggcgtta actttcagac cgagactagc
    20281 ggcaaaggtc gcggctttgg cgatacgcac cagctcttgc gcctgttcgg cgtcagtttt
    20341 ggcatcagca tagcaaccgg tgtggatctc gataaacggt gcgccaacct ctgccgcagc
    20401 tttgatctgc tcttcatcgg cgtcgataaa gagagaaacc tgaatcccgg catctgtcag
    20461 acgtttgcag gcatcgcgca ttttttcacg ctgccctgca acgtccaggc cgccttcggt
    20521 tgttacttcc tgacgctttt ccggtaccag gcagcaaaaa tgtggcttcg tctcaacggc
    20581 gatcgccagc atctcttcgg tcactgccat ctccagattc attcgggtat ccagcgtctg
    20641 ccgcaggatg cgcacatcgc ggtcagtaat gtggcggcga tcttcacgta aatgcacggt
    20701 aatgccgtcc gctcccgcct gctcggcaat aaacgccgcc tgcaccggat ccgggtaagc
    20761 agtaccgcgt gcgttacgca gcgttgcaat atggtcaatg ttgacgccta acagtaattc
    20821 agccatgaca atcctcatca ttcataatgt gttttcaccg ttcgcttagg cataaactgc
    20881 cggaacagtt ccctgctctt taatggttta ccgccaagat acggcttaag cgccatgcgg
    20941 gtaaagcgtt tcgcggcgcg tagtgtgtct gcgtcaggaa attcccgcgc gtttaacgct
    21001 tttaactgcc ttccggtgaa cgttttattg tcgataacga cgcttgcgat aaaccctttt
    21061 tcttcgcgat aacgatacgt catggtgtca tctaccggct cgccgctacc cgcgcaatag
    21121 gtaaaattga cgccatagcc cagatgcccg agcaatgcca gttcaaagcg gcgcagcgcg
    21181 ggttctggcg taccagtggc acctgcaaga gactgaatgc agtgcaaata atcgaaaaag
    21241 agttcagaga agcgcgtctc gtattccagt acgcgggaga gaagttcgtt gatgtacaga
    21301 ccgctgtaaa gcgtgatacc gcttaatggc agcgccagcg agacagcttc agcactgcgc
    21361 agcgttttga cttcgccacg cccgccgaaa cgtagcaaga gaggggtgaa aggctgtaat
    21421 gcacctttca gagtagagcg tttagagcgt gcgcctttgg caaccagacg cacgcgaccc
    21481 gattcctccg tgaagacgtc cagcatcagg ctggtttcgc tccacgggcg actatgcagg
    21541 acaaatgcgc gctgccagcc ttccatcgga gtgactctta aagatcgtca acgtaaccga
    21601 gactgcgcag tgcgcgttcg tcgtcggccc aaccggattt cacttttacc cacagttcaa
    21661 ggtggacagg cgcttcgaac atttcctgca tgtctttacg cgcttcaatc ccgatggttt
    21721 tgatcttggc ccctttgttg ccaataacca tcttcttctg cccttcacgc tcaacgagaa
    21781 tcaaaccatt gatgtcataa ccaccgcgtt cgttagagac gaaacgttcg atctccacgg
    21841 tcacggagta cggcagttca gcgccgagga aacgcatcag tttttcgcgg atgatttcag
    21901 acgccataaa acgctgtgag cgatcggtga tgtaatcttc cgggaagtga tgggtcgctt
    21961 caggcagatg cttacgcacg attgctgcga tagtgtcgac attcagcccg gtttcagcag
    22021 agatcggcac gatatcgagg aagttcatct gacttgccag gaactgtaag tgcggcagca
    22081 gatcggcttt ttcctgcacg ttatccactt tgttcaccgc gaggatcacc ggcgctttgc
    22141 catcgcgcag tttgttgagc accatttcgt cgtccggcgt ccagcgggtg ccttcaacga
    22201 caaaaatcac cagctcaacg tcgccaatag agctgctcgc cgctttgttc atcaggcggt
    22261 taatagcgcg tttttcttcc atatgcaggc caggggtatc aacgtagatc gcctgatacg
    22321 cgccttcagt atggatcccc acaatgcggt gacgagttgt ttgcgctttg cgggaggtga
    22381 tggagatctt ctgccccagc agtttgttca acaatgtgga tttgccaacg ttcggacgtc
    22441 cgacgatggc aataaatccg cagtaacttt tatcgatgct cattccagct ccagtttttt
    22501 caacgcctgt tcggcggcag cctgctcagc cttacgacgg cttgaacctg tgccaaccac
    22561 cggttcactc aggccgctga cctggcagtg gatagtaaat tcctgatcgt gcgcttcgcc
    22621 acgtacctgg actaccagat aagtcggcag cggcagatgg cgaccctgca aatattcttg
    22681 caagcgcgtt ttcggatctt tttgtttatc gcctgggcta atttcgtcca aacgagtttg
    22741 ataccagttg aggattaatt tctcgacggt ttgaatatca ctgtcgagga atacgccacc
    22801 aattaatgct tcgacggtat cggcgagaat tgactcacga cgaaatccac cgcttttaag
    22861 ttcaccaggc cctaaacgta agcactcgcc taactcaaat tcgcgcgcca gttccgccag
    22921 cgtattgcca cggaccagcg tggcgcgcat ccggctcata tcgccttcat ccacacgagg
    22981 gaaacggtga taaagcgcat tggcgataac gtagctcaga atagagtcgc ctaaaaattc
    23041 taaacgctcg ttatgtttgc tgctggcact acgatgagtt aatgcctgct gcaacagttc
    23101 ctgatgatta aaagtgtagc ccagcttccg ttgaagccga ttaattacga tggggttcat
    23161 gcgataccaa taaataaatg cgtcaacaat tcagcacacg aaacagacct gatatacatg
    23221 gctctgctaa ctgcttcgct gcagtttcag tttaccggta tatggggacc aacgctgttt
    23281 cgtgtgccgt ggcaacctgg aggtgccaac cttaaacttc gggggaatat tctatacaca
    23341 acgacggggg atgtcgttag ccacgggaga tttatctcat aaataattca cgttgtcgcc
    23401 ataacggcga caacgtgaac gaagatgggc tattaatgga tgccgccaat gcgacttaag
    23461 cgcacacctg tcggccattc gccttcttgc ttatcgaaac tcatccagat agccgttgcc
    23521 cgaccgacca gattcgcttc aggcacaaaa ccccagtaac ggctgtccgc gctgttgtcg
    23581 cggttgtcgc ccatcatgaa gtattgtccc ggcggaacaa tccaggttgc cagttgttgc
    23641 cctggctgct ggtaatacat ccccacctgg tcctgcgcaa tcggcactgt cagaattcgg
    23701 tgcgtcacat cacccagtgt ctctttacgc tcggaaagac gaattccatt ttctttggtt
    23761 tcgtttttcg gcacttcaaa gaatccgctg gtcgcttccc caccattacg gcgtgagaag
    23821 gtctgaacga aatcgctcgg ttccacgttg gagtaggtga ccggtagcgc gttttcacac
    23881 gcctggccgg aactgcatcc cggttgaatc gtcagctctt ttgagaccgg atcgtaagtg
    23941 actttatcgc ccggtaaacc caccgcgcgc ttgatgtaat caagctttgg atcttccgga
    24001 tatttaaaga ccacgatatc gccgcgtttc ggatgaccgg tttcgatcag cgttttctgg
    24061 tagataggat ctttaatgcc ataagcaaac ttctctacca gaataaaatc accgattaac
    24121 agagtcggca tcatcgaacc tgacgggatc tggaacggtt cataaataaa cgaacgtaca
    24181 atcaatacga tagccagcac cggaaaaaca gaagcaccgg tttccagcca gccaggcttc
    24241 ggcgcaacct ttttcagggt tgctttatcc agtgagtcac cggcagccgc ctgcgccgct
    24301 gcctgacgtt cccgccgttt aggtgcgaaa aagaatttat ccacgcacca taaaatgccc
    24361 gtcaccagtg tggcaatcac cagaatcagg gcaaacatat tcgccatgcc aactcctaag
    24421 ggttatttgt tgtctttgcc gacgtgcaga atggcgagga acgcttcctg cggcagctcg
    24481 acgttaccga tctgcttcat gcgtttctta ccttctttct gcttctgcag cagctttttc
    24541 ttacggctga tatcgccgcc ataacattta gccagtacgt ttttacgcag ctgtttcacg
    24601 gtggatcgcg caataatgtg cgtaccaatc gctgcctgaa tggcgatatc aaactgctgg
    24661 cgtgggatca gatctttcat cttctccacc aactcgcgac cgcggttttg cgaattatca
    24721 cggtgggtga tcagcgccaa cgcatcaaca cgttcaccgt tgattaatac gtctacacgt
    24781 accatgtcgg acgcctggaa gcgtttgaag ttgtaatcca gagacgcata accacgcgag
    24841 gtagatttca ggcgatcgaa gaagtcgagc accacttccg ccatcgggat ctcgtacgtc
    24901 agcgccacct gattaccgtg gtaaaccata ttggtctgca cgccgcgttt ttctacgcac
    24961 agcgtaataa cgttgccgag atatgcctgc ggcagcagca tgtgacactc tgcaatcggc
    25021 tcgcgcagtt cgtaaatgtt atttaccgca ggcagcttgg atgggctgtc gacgtagata
    25081 acttcccttg acgtggtttc aacttcatac actacggtcg gcgcagtggt gatcagatcc
    25141 agatcgtatt cacgttccag acgttcctgg atgatctcca tgtgcagcag gccaaggaag
    25201 ccgcagcgga aaccaaagcc cagcgcgctg gagctttccg gctcatagaa cagtgaggca
    25261 tcattcaggc tgagtttacc cagcgcgtca cggaaggctt catagtcgtc ggaacttacc
    25321 gggaacagac cggcgtatac ctgcggtttg actttcttaa agccaggcag cgccttttct
    25381 gccggattac gcgccagcgt taaggtatcg ccgactggag cgccgtggat atctttaatc
    25441 gcacatacca gccagcctac ttcgccacat ttcagttcag tgcggtcaac ctgtttcggc
    25501 gtgaagatgc ccagacggtc ggcgttatag gtctgcccgg tactcatgac tttcactttg
    25561 tcgcccttac gcagggtgcc gtttttaata cggataagtg aaacaacgcc caggtagttg
    25621 tcgaaccatg agtcgataat tagtgcctgc aacgggcctt ccggatcgcc ttccggcggc
    25681 ggaatgtcgc gcaccagacg ttcgagaacg tcctgcacgc caacgccggt tttcgctgaa
    25741 cagcgcaccg cgtcagtggc gtcgatgccg acgatatctt caatttcttc cgccacgcgt
    25801 tcaggatcgg ctgccggcag gtcaatcttg ttcagtactg gcaccacttc gagatccatt
    25861 tccatggcgg tgtagcagtt tgccagggtt tgcgcttcca cgccctgccc ggcgtcgacc
    25921 accagcaatg caccttcaca ggcagccagc gaacgggaaa cttcatagga gaagtctacg
    25981 tggcccgggg tgtcgataaa gttaagctga taggtttcgc cgtcagacgc tttgtagtcc
    26041 agcgtcacgc tttgcgcttt gatggtaatg ccacgctcac gctcaagatc catggaatcg
    26101 agaacctgcg cctccatttc acggtcagac aggccaccgc agatctggat aatacggtca
    26161 gacagcgtcg atttaccgtg gtcaatgtga gctatgatcg aaaagttacg tatattcttc
    26221 ataaagtatg attattgtgc cttaatgccc ggttaaccag gcttttagaa gtcgctgttc
    26281 tgagcttaac gtctgtatta atagaaacgc cgcattctac actacaacat tgaggcgagg
    26341 aaatgttcat accgtatgga ttgtggtatc tggaaacgtc ctcgcatttg ttatgcaaaa
    26401 tgcaacaaag ccagtgaaat cactggctcg cgtcttccga agatgtttca aatcgcacaa
    26461 ggccaggcgg caatgccacg cttaagatga tcggttgcca ttctgcccgg gcagcaaact
    26521 tacgcgagta gccgcgcgca atcaggaacc cgccaatacc gccgaggacc gcaccacata
    26581 atgctgcgac gtcagaagca aagagtagct gaaatagcga agcgatgagg aataatccca
    26641 ccagcggcga catataaacc agtaatgcgg agctaagcag gctgccttcg gcgatcccca
    26701 attccacttt ttgccccggc actaacggtt catcacaggg tacgacaatg gtatgcgtgg
    26761 tttgcgggcc aagtttattc aacacgcggc taccgcaacc ggcgcgtgaa gcgcagctgc
    26821 tgcatgaagc tttaacatca caactgacca gcgcctgccc gttttgccag gagacgacgg
    26881 tagcccactc tttgatcatt gcgctgcccc gaacttaata ttctcggcaa tgcgtttcgc
    26941 cgtttgcggc ggcagttcac cgacaatggt gatttcggcg ttatcacgta cgcttgtact
    27001 gacggttctg cgtccggtgc gcaacatctg atcggtgctc gatggcgtag cgcggttaac
    27061 gtttaccgag aagctgaata atccgtcaga atagagacgt gattcgatag gcatgttgtc
    27121 catcgtcggt agcggacgtc gactactgga aacttcgcta aaaccctgtg gcaaccaggt
    27181 tggcgtccag ctgaatttag ctttttcacc tacaggaaca gaaagcaacg gcggcaaatt
    27241 tgccttcgcc agcgtctgca tactgctgct gatatcctga ttgacgttaa aagcaatcac
    27301 gcgaaattgt tccagcgttt caccatcgcg atcaaggaga tcaacccgca tcggtaattt
    27361 cgattcggtg tccatccaca cgatgtagct gtagcgtgta ccatctcggg caaccacgcg
    27421 aatgacttcg caaagacgat cagcaatacg cgtgcgcccg acggagataa agtcgtagta
    27481 aggagaaagg cgtttgaaat cggtatagat gagcgatggc agagaatcaa caatgtaatc
    27541 gccattaagc gtgaacggtt caagtcccgg ttcaaaatag ctgatttcat cgccgcgctg
    27601 taccacttcc cggcgcgggc catccatttg caacaattgt gcaagaggac ggttatcgag
    27661 gcgtgcatgt cgataacgca gagactcaac accctgttta ttgatgctga tgaatgacag
    27721 ctcgtaattc agtgactgac tggccaggtt catctgctgt aataacgccc cggacgcggg
    27781 agtggccgag gcgttagcag agaataacag gctacctgtc actaatgaca tggcaaacca
    27841 aagttgcttc attactgcga ttgcgttcct aaagtttgaa ttcctggcac ctgtacagcg
    27901 gcttgctggg tttgcgcctg ctcaaactga agctgttcag agtggagtcg gcgttgcagt
    27961 tcgtaatcct gcaacattgc attaatgcga cgacgctgct cctgtacctg ctgctgttga
    28021 ccattgtttg cggtcgcttc agaaggtact cccaggctta ccgggctggc tttacccatc
    28081 atcggcagtg tattaaatac cggcgtttcg ggctgctggg acgtttcaga ttgtccatta
    28141 tagtgctgga cgccaacgat aactgcaagc gatacgcatg ctgctacgcc catttgggta
    28201 agctgtgccg cccacggacg tactttctgc cagaatggca ttttctgcca ttgatgcggc
    28261 gcaggctggg cttccgggat caatgtcgcc ggttgacgta ctggctcttc ttcaatggcg
    28321 gccatcacgc gtgaagagat atcgaaatgg agcacctcgg gagtatcacc ccgcattgag
    28381 tcacggatta agtgatagct ttcccaggtt ttctgcattt ctgggttatg agccagttcg
    28441 ttaagcagct cactatccag cgtttcgcca tccattaaag cggaaagttg ttctttctgc
    28501 atgcctaata cccttatcca gtatcccgct atcgtcaacg cctgataagc ggttgaactt
    28561 tgttatcaat agcttccctc gctcggaaga tacgtgaacg caccgtacct accggacaat
    28621 ccatgatagc ggctatctct tcatagctca ggccatccag ctcccgcaag gttattgcca
    28681 tgcgcaaatc ttccgggagg gactcaatag ttcggaaaac tatctgtctc agttcttctg
    28741 acaacattaa gttctcaggg ttcgaaattt ctttcaacgc gccgccactt tcgaagtttt
    28801 cagcttcaat ggcatccaca tcactggaag gtggacgacg cccctgagca accaggtaat
    28861 ttttcgctgt atttacagca atccgataca gccatgtata aaaagcgcta tctccccgga
    28921 acgaatccag cgcacgatag gctttaataa aagcttcttg taccacatcg ggaacatcac
    28981 ccgacggcac atagcgggaa accagactcg ccactttatg ctgatagcgc actaccagta
    29041 agttaaaggc tttctgatct cccttctgga cccgttcaac caggacctgg tccgttaact
    29101 gctcgctcat ccgaggtaaa gtctccccaa accaaatttc cacgcgctat cgaaacgcca
    29161 ctccattagc tgcaatttga gcaagcaaag ggttagagtg tctcgttttt gtaaagttcc
    29221 gtaacgcatc tgtttttgtt tgtcatgctg tagacggatc attatctatc attataagtc
    29281 tacagaatct gaacatcgca ttatctgtgt agaaatgccc atttaactgc ctgaagagta
    29341 acccaacggc ctttttattt caccacctaa tcctccacca gccagtaact tctctttttc
    29401 tcgccgccct gcatcagcgt gtttagcaac tgtaacaaat attaaaatag caggtgttta
    29461 ttcgcacaac atgatgctat actgaccaaa ccgtgtttag taaattaaac aaagaaaatg
    29521 aatactctcc ctgaacattc atgtgacgtg ttgattattg gtagcggcgc agccggactt
    29581 tcactggcgc tacgcctggc tgaccagcat caggtcatcg ttctaagtaa aggcccggta
    29641 acggaaggtt caacatttta tgcccagggc ggtattgccg ccgtgtttga tgaaactgac
    29701 agcattgact cgcatgtgga agacacattg attgccgggg ctggtatttg cgatcgccat
    29761 gcagttgaat ttgtcgccag caatgctcgt tcctgtgtgc aatggttaat tgaccagggg
    29821 gttttgtttg atacccacgt tcaaccgaat ggcgaagaaa gctaccatct gacccgtgaa
    29881 ggaggacata gtcaccgtcg tat
//
LOCUS       DAJTYD010000032        29716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTYD010000032 DAJTYD010000000
VERSION     DAJTYD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.032"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..426)
                     /locus_tag="OO585_001292"
                     /pseudo
     CDS             complement(1..426)
                     /locus_tag="OO585_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(401..1201)
                     /gene="otsB"
                     /locus_tag="OO585_001293"
                     /pseudo
     CDS             complement(401..1201)
                     /gene="otsB"
                     /locus_tag="OO585_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1368..2354)
                     /gene="araH"
                     /locus_tag="OO585_001294"
     CDS             complement(1368..2354)
                     /gene="araH"
                     /locus_tag="OO585_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6628944.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2369..3883)
                     /gene="araG"
                     /locus_tag="OO585_001295"
     CDS             complement(2369..3883)
                     /gene="araG"
                     /locus_tag="OO585_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6628945.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGAIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3953..4941)
                     /gene="araF"
                     /locus_tag="OO585_001296"
                     /pseudo
     CDS             complement(3953..4941)
                     /gene="araF"
                     /locus_tag="OO585_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5655..6158
                     /locus_tag="OO585_001297"
     CDS             5655..6158
                     /locus_tag="OO585_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6628946.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6237..6488)
                     /locus_tag="OO585_001298"
     CDS             complement(6237..6488)
                     /locus_tag="OO585_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6628947.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6603..6689)
                     /gene="azuC"
                     /locus_tag="OO585_001299"
     CDS             complement(6603..6689)
                     /gene="azuC"
                     /locus_tag="OO585_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6628948.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            6952..7275
                     /locus_tag="OO585_001300"
     CDS             6952..7275
                     /locus_tag="OO585_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6628949.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7446..7943
                     /gene="ftnA"
                     /locus_tag="OO585_001301"
     CDS             7446..7943
                     /gene="ftnA"
                     /locus_tag="OO585_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6628950.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(7981..8220)
                     /locus_tag="OO585_001302"
     CDS             complement(7981..8220)
                     /locus_tag="OO585_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6628951.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8411..9622
                     /gene="tyrP"
                     /locus_tag="OO585_001303"
     CDS             8411..9622
                     /gene="tyrP"
                     /locus_tag="OO585_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6628952.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(9684..10349)
                     /gene="yecA"
                     /locus_tag="OO585_001304"
     CDS             complement(9684..10349)
                     /gene="yecA"
                     /locus_tag="OO585_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6628953.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10545..10631)
                     /locus_tag="OO585_001305"
     tRNA            complement(10545..10631)
                     /locus_tag="OO585_001305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10595..10597),aa:Leu,seq:taa)
     gene            complement(10644..10717)
                     /locus_tag="OO585_001306"
     tRNA            complement(10644..10717)
                     /locus_tag="OO585_001306"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10683..10685),aa:Cys,seq:gca)
     gene            complement(10772..10847)
                     /locus_tag="OO585_001307"
     tRNA            complement(10772..10847)
                     /locus_tag="OO585_001307"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10812..10814),aa:Gly,seq:gcc)
     gene            complement(10999..11547)
                     /gene="pgsA"
                     /locus_tag="OO585_001308"
     CDS             complement(10999..11547)
                     /gene="pgsA"
                     /locus_tag="OO585_001308"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6628954.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11604..13436)
                     /gene="uvrC"
                     /locus_tag="OO585_001309"
     CDS             complement(11604..13436)
                     /gene="uvrC"
                     /locus_tag="OO585_001309"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6628955.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13433..14089)
                     /gene="uvrY"
                     /locus_tag="OO585_001310"
     CDS             complement(13433..14089)
                     /gene="uvrY"
                     /locus_tag="OO585_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6628956.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14384..14560
                     /locus_tag="OO585_001311"
     CDS             14384..14560
                     /locus_tag="OO585_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628957.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            14547..14771
                     /locus_tag="OO585_001312"
     CDS             14547..14771
                     /locus_tag="OO585_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6628958.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14838..15560)
                     /gene="sdiA"
                     /locus_tag="OO585_001313"
     CDS             complement(14838..15560)
                     /gene="sdiA"
                     /locus_tag="OO585_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6628959.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(15790..16542)
                     /gene="yecC"
                     /locus_tag="OO585_001314"
     CDS             complement(15790..16542)
                     /gene="yecC"
                     /locus_tag="OO585_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6628960.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(16539..17207)
                     /gene="tcyL"
                     /locus_tag="OO585_001315"
     CDS             complement(16539..17207)
                     /gene="tcyL"
                     /locus_tag="OO585_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6628961.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17222..18208)
                     /gene="dcyD"
                     /locus_tag="OO585_001316"
     CDS             complement(17222..18208)
                     /gene="dcyD"
                     /locus_tag="OO585_001316"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6628962.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18313..19113)
                     /gene="tcyJ"
                     /locus_tag="OO585_001317"
     CDS             complement(18313..19113)
                     /gene="tcyJ"
                     /locus_tag="OO585_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6628963.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19201..19752)
                     /gene="fliZ"
                     /locus_tag="OO585_001318"
     CDS             complement(19201..19752)
                     /gene="fliZ"
                     /locus_tag="OO585_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6628964.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19798..20517)
                     /gene="fliA"
                     /locus_tag="OO585_001319"
     CDS             complement(19798..20517)
                     /gene="fliA"
                     /locus_tag="OO585_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6628965.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(20837..22543)
                     /locus_tag="OO585_001320"
     CDS             complement(20837..22543)
                     /locus_tag="OO585_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6628966.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            22809..24215
                     /gene="fliD"
                     /locus_tag="OO585_001321"
     CDS             22809..24215
                     /gene="fliD"
                     /locus_tag="OO585_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6628967.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24240..24650
                     /gene="fliS"
                     /locus_tag="OO585_001322"
     CDS             24240..24650
                     /gene="fliS"
                     /locus_tag="OO585_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6628968.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            24650..25016
                     /gene="fliT"
                     /locus_tag="OO585_001323"
                     /pseudo
     CDS             24650..25016
                     /gene="fliT"
                     /locus_tag="OO585_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            25094..26581
                     /gene="amyA"
                     /locus_tag="OO585_001324"
     CDS             25094..26581
                     /gene="amyA"
                     /locus_tag="OO585_001324"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6628969.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26615..27028)
                     /locus_tag="OO585_001325"
     CDS             complement(26615..27028)
                     /locus_tag="OO585_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6628970.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27215..28420
                     /gene="yedE"
                     /locus_tag="OO585_001326"
     CDS             27215..28420
                     /gene="yedE"
                     /locus_tag="OO585_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6628971.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            28417..28650
                     /gene="yedF"
                     /locus_tag="OO585_001327"
     CDS             28417..28650
                     /gene="yedF"
                     /locus_tag="OO585_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6628972.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            28757..29428
                     /locus_tag="OO585_001328"
     CDS             28757..29428
                     /locus_tag="OO585_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6628973.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(29468..>29716)
                     /locus_tag="OO585_001329"
     CDS             complement(29468..>29716)
                     /locus_tag="OO585_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6628974.1"
                     /translation="QVFAVPPACTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADV
                     NGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         7378 a         7661 c         7115 g         7562 t
ORIGIN      
        1 ccccacgttt gcgtaattca tgcgcaaagg gcaacaggtg ataatcgtgg atccagataa
       61 tgtcatcgtc ttgcaacagc ggcagtaatt tatctgccag caacgcattt acgcgtagat
      121 agccgtccca ggcaggacgc tgaaattgca tcagatcgag ccgataatga aaagcgggcc
      181 agagaacggc attggagaat tggttgtagt attcgtcaag gtcctgttcg ctgaggttaa
      241 aagaggccca cgtaatgtta ccttttttag cggctgatcc tcattccctg tttcaccact
      301 ccagccaaac cacagtccgc ctgtggcttt cagtgccccc agtatgccaa cggcaaggcc
      361 accggcactg gcggcgtgct cgtctggtgg tgcaatccgg ttagatacta cgactaaacg
      421 actcatagtc atcactcctg ttattttctc ttttttgttg taatgcggtg gttatcattt
      481 caagcctgct ccagacatcc ggcacacccg ccagtcgcca tgatgcctga gttgcacctg
      541 tgccaatttt tactgacatt ccgcccagtc ggttaacgac tgcgaagcca gattcatcgg
      601 ttaaatcatc gcccagaaat acgggcgttc gcccgataaa gggagcttcc tgcataaaag
      661 ctgcaattgc ctcaccttta ctggtacctc tcggtttgat ctcgacaaca cactttccct
      721 gctgtaacgc catttgtggc cagatctgag taatacgtta cgctaatgtc attaatgcgt
      781 cttcatgctg cggagcctga cgataatgca gcgcaaaagc catccctttc gcctccagct
      841 ccgcgccggg atactgagcg ataactgtat gcagttgcac gctaatatca cgcgcaatcg
      901 catccggcag atgaacgata tgcgttttac cattgatgtc acggcgctcc gccccatgca
      961 cgcccgctaa cgggaagcga taaggttttg ccagtgcgtc aagctccacc attgagcgcc
     1021 ctgatatcaa tgccaatgca ccatcacttg cggttgccag tagctgtagt ccttgcagaa
     1081 tattgtcagg cacgacgacc tgatcgggat gcggtttgat ttccgccagc gttccatcga
     1141 gatcaaaaaa ccaggcatat ttcgcggata gttcaggggt ttcggttaac ggttctgtca
     1201 cccggttctc ctacttctct tttcttattg ttttgcgcag aacgcgcttt acagacatgt
     1261 aagtatagac agtgtgacgg gggtcgccat ttgcaacgaa gaacagccaa ccgttatggc
     1321 ggttggctgt ggtgggaaaa aacgctaaat tgttgcagaa aaaagcatca gacagtgcgt
     1381 ttcgcttttt gcttgtaacg gtcgaagatc accgctgcca gcaggattaa gccgcgaacc
     1441 acgtactgcg cgaaaggaga aatattaagc aggttcatgg cgttttccac ggtgcctaaa
     1501 attaagatac ccgccaccac atatgagatt tttccgatgc ctcctttcag agaaacgcca
     1561 cctaaaacac aggcggagat aacaatcagc tcgtaaccaa tcgacgtcat tggctgccca
     1621 ctggtcatac gtgaagccag aataattccg gctatcgcag ataccaggcc agagagaaca
     1681 aagataataa ttttggtgcg aacaaccggt acacccgcca gacgcgcagc ctcttcgttc
     1741 ccgccaatcg ccagggtgtt acgaccaaag gtggttttat tcagcaacaa accaaagata
     1801 atcagacacg cgacggtgag ccagattggc gcaggcagac cgaaccagtt agcgtaacca
     1861 agggcaaaga agctttcatc ttcgataccg accgctttac cgtctgaaat gatgtacgcc
     1921 agacctcgaa caatctgcat cgttgccagt gttgtgatca gagcatttat tttcagtttg
     1981 gcgataacaa agccattgac caggccacag agaatgccaa gcagcaaccc cgctgccacg
     2041 ccaatccaca ggctttcagt caggttgata accaccgccg tggtgacacc cgcacaggca
     2101 attacggagg cgacagaaag gtcaaagtca ccggaagcaa ggcagaacaa catgccacac
     2161 gccaccatcc ccgacatgga aattgccagg cccaaccctt tcatattaat gaaggtggca
     2221 aaatttggga caaaaatggc acaggcgata aagagcaccg caaacaccac cagcatgccg
     2281 tactgatccc agatacgccc gaagctgaat gacgacttag gtgcgccaga acccgatgta
     2341 gaaacagaag acatcatact ctccttactc aggcaactgc ctggctgact ttaggcatcg
     2401 caaggctcag tgcctgacgc tcatctgcct gctcgtgtaa caattcaccg gcgattgcac
     2461 cttcccgcat caccacaatc cggtcggcaa cgccgaggac ttcaggtaag tcgctggagg
     2521 caaacagcac cgccacaccc tgcgccgcca gcgcataaat tacgttatat atttcgtgct
     2581 tagcaccaac atcaatgccg cgcgtaggtt catccagcaa aatgaccttc atctcttccg
     2641 ataaccagcg gcccagaatg gctttttgct gatttccgcc tgagagattc atgatcagtt
     2701 gctcagcgcc cggcgttttg atgttgagcg aacgaatgtg ctgatcggca ttgttttctt
     2761 cccaaccgtt gttgattaca caaccgccga gcacatgttt gcgtctggca ctgatgttga
     2821 tattgtcgcg aacggagtgc acgggaataa tgccttccgc tttgcgatct tccgggcaga
     2881 gcatcatgcc tgcggcaatg gcgtggctcg gtttacgaat atcgatcggc tgttggtcga
     2941 tataaacctg accggcggtg atttgcgtcc cgccaaacaa gcctttcatt aattcgctac
     3001 gccccgcccc taccagacca aacagcccaa caatttcacc actgcgaacc gccagactta
     3061 ttggcgtacg cacgcctggt gctttcacag catcaagacg caggcgctcc tcgccataac
     3121 tacgaggttg ccagccgtag atatcgccaa tgtcgcgccc gaccatcgcc tgcaccagcg
     3181 cgtcgtggtc aacctgctgc atatcggtaa aggttttgac ataacgtcca tctttaaaga
     3241 cagtgatggc atcgctgagg gcaaatattt cttccatacg gtgagaaacg tataagatca
     3301 cccgcccctc ttttcgcagt tcacgaataa cgcggaaaag attgtcgatt tcgcgggcag
     3361 aaagggagct ggttggctca tcaaaggcga taattttagc gttacgcgcc agcgccttgg
     3421 cgatttcaac catctgccac tgaccaatgg agagatattt cagcggcgtg tccgggtcaa
     3481 tatccatacc aagatgttta agttgtaaac ccgcctcata attcagcaat gagcgattca
     3541 caatgccgcc tttatgcggc agctggccga gatagatgtt ttccgcgacg gtcatttccg
     3601 gcacgagatg cagttcctgg taaataatcg ccacacccgc gttaagtgct gcggtcgtgt
     3661 cggaaaagga catttcctgc ccattaatca ctacagaacc cgtggttggc gcatagttgc
     3721 cgctgaggat ttttaagaga gttgattttc ctgcgccatt ttcacccatc aacgcatgaa
     3781 cctgaccggc atagcagtca aaactaatat ccgtcagcgc cttaacgccg ggaaacgttt
     3841 taccgatgcc gcgaaatgag agatacgggg tagactgttg cataacgtct ccgtgaatca
     3901 ctggtcgttc acaccccctc tgcatcatgc agaggggaat ttttccggca aattacttac
     3961 cgcctaaacc ttttttctcc agttcttctt taaagttgtc acgcgtgatc agcaccacgt
     4021 cggtaacttc ggtaaatttt ggcggttcaa cgtcttttgc tacccagttg taaagcattt
     4081 cgctggattt atagccatgt acgtccgggc ttggcagcag ggaaccgtag aagccggttg
     4141 cctgtacttt agacagttcg ctcaccgcat ccacaccgtt aatgccaatg ccgataatat
     4201 cggcagcttt aaagccctga ccttccgtcg cgcgtacgcc gcccagcacg gtgctgtcgt
     4261 tcataccgac gatcagccaa tgtttaactt caggatgttg aaccagcatt gagttggcag
     4321 cgtcaaatgc ccccgggatg tcgttagatt tggtaggtac ctgataaatt tgtttttccg
     4381 ggaatccggc cgctttcagc gcatccatag atcccgtagt acggcggcgg gcagtatcca
     4441 gttcgttggc ggtaatcacc atcaccgcgc tttctttgac atcccagcca cgtttctgca
     4501 tctctttata cagttcctga ccctgacgtt cgccaatttt agtcgcgcca tcatcaccag
     4561 cggaacggta tccattggct tacctttggc gttaacaaac tgatcatcca cggcaatgac
     4621 tttcatatcg tagccacgcg ctttcgcgac gatggcagag ccgagtttgg ggtccggagt
     4681 acaaataacg aaaccttttg cgccactggc agccaggctg tcgatcgcgt tcaatgtttt
     4741 ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct aaatccttcc cggctttatc
     4801 ggcaaacttc cattcggtct ggaaccacgg ctcttccggt tgcttcacca gaaaaccgag
     4861 cttcaggttc tccgccatag cggattgtga cataacggct gccagaccaa tggctgccag
     4921 ggctttagta aatttgtgca tggttctctc cagctttagt gtcgttttgt gtagggcaaa
     4981 aacgaatgac attcgttaaa ttaatcggaa aacaaagcat taccttttaa ctaaaagata
     5041 agtgaccgtg ttgacatagt tttagcgaga aattaattct ccataggaga gcaatatcac
     5101 atcgcagaat tacagtgaga acgtgcataa atttagcggg aaaagacata agggaaagcc
     5161 aatttgtcag acaaattgtc gaatgcacag cagattaatc cataagatta gcttggaaat
     5221 ccttgttgtc tttggtaccc atgcgggatg tcttcttttt aaccagtcaa taggccgcat
     5281 tacctggcgt tgagtttttg aaatggtgta ataaccgcaa ctcaaagatg tggaaaatgc
     5341 acgtcattca tttcgccatt aattatcact gtgctcatta attaacagaa cacgtataat
     5401 gagagccatc tcgcaaaaat gaaaaaacgt tttataaaat catcatttca tcaatgaaat
     5461 gtaaaaatat ataaacttga tgatttaagc attttcttat acccgttcag acgttattct
     5521 tatttcagat catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat
     5581 tattctgcaa acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata
     5641 taaggatatt aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc
     5701 gcgagtttta cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc
     5761 tgaacggcac cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc
     5821 gcatgtttaa ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc
     5881 ccggtgaaaa actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc
     5941 aacgttctag tacgttggca cagttagccg atgaagcgaa agaactgaat gatgattcaa
     6001 ccgtcaattt cctgcgcgat ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa
     6061 ccattcttga tgaagtgcgc agtgcgaaac ttgctggtat gtgcccagtg cagaccgacc
     6121 aacatgttct gaatgtcgtg tcgcatcagc tgcattgatc atacatcggc gctaatgcat
     6181 tgcgccgatg aagtttttga gaaaccgctg cctcatcttt ttgaagcagc ggttttttaa
     6241 tgggattcgc cttgtggggt aaacttgagt tcaatcagcg cgatggcttt ttggattgcc
     6301 cgcatggtga ccgggtctgc ggcggcggga tggttactaa agtcgatatt cttcagctga
     6361 ctggacattt tttcacgaac ttcaacgggc gcgattacat cgagaacatc cagaatttgt
     6421 ttgataacca actggcaagc aaccacatca gaaaccagtt cctgatcggc attcagcggc
     6481 tgggacatcg taaactcctg atagcatttt gaaaaccgtt atagtagcgg cttcacatct
     6541 tcagcgatag tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt
     6601 tgttatcgga acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac
     6661 atacttttca ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacatgtg
     6721 ttattttctt tgtgcttata atatgacaac catcacaaaa atcaatcttt atgtgatgca
     6781 aatcacacaa ataccccctt aatgttataa aaatgataat caaaaaacag cccccccatt
     6841 tctgacacct acagatggca agaaatagcg cctgccaggc gtcttttccg gtcattgtcg
     6901 cagcgctgta acgcgtaaaa tagtgctttc ttttactctt ctggctggac catgagactt
     6961 ctgattctga ctctttcatt aataacgctg gcggggtgta cggttactcg ccaggcccat
     7021 gtgagcgaag ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg
     7081 cagcatgccc ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag
     7141 gaaggctata cccacgcaat cccctttggt cagccagtag gcaactgtag cctttttgcg
     7201 ggttccttat gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc
     7261 cctgtctttc tctaaatatt ttaatcagcg aggggatctt cgctgattaa aaaaatagcg
     7321 gtaatgcgtt ttattcccat tcgtattttt aataattgaa gtttatattt taccttttgc
     7381 aaataataaa ataacaaatt atagtgacgc cacagcaaca aatataacct ttgtggagca
     7441 ctatcatgct gaaaccagaa atgattgaaa aacttaatga gcagatgaac ctggaactgt
     7501 actcttcact gctttatcag caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg
     7561 ctgccgcgtt cctgcgccgt cacgcccaag aagagatgac gcatatgcag catctgtttg
     7621 attacctgac tgataccggc aatttaccgc gtattaatac cgttgaatct ccgtttgctg
     7681 aatattcctc acttgatgaa ttattccagg aaacctataa acacgaacaa ttaatcaccc
     7741 agaaaattaa cgaactggct catgctgcaa tgaccaatca ggactaccca acatttaatt
     7801 tcctgcaatg gtatgtttct gagcagcatg aagaagagaa actgttcaaa tcgattattg
     7861 ataaattaag cctggcaggc aaaagcggcg aaggtctgta ttttatcgac aaagaactct
     7921 ctaccctcga cacacaaaac taatactctc cggcggcagg tttactgccg ccgtttcaaa
     7981 tcagtgacgg caaatcttac tctgatcggt cgaaaaaccg tctttcgaag gtataaattt
     8041 gccttttgct gccagaaacg ctaccagctc tcccgctgtc atcccttctg ccgagcaggt
     8101 gtgaaaacgt gcctgttcac caaaacgcgc tttaattgct gcttccagac tggcatgcgt
     8161 atattgctcg cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat
     8221 cgttcctcct caaaaaaagc ctagcgtagc gattgccgct tatgaagact ttgcgccaga
     8281 gcagaactga atgcttttta ttgtacattt atatttacac catatgtaac gtcggtttga
     8341 cgaagcagcc gttatgcctt aacctgcgcc gcagatatca ctcataaaga tcgtcaggac
     8401 agaagaaagc gtgaaaaaca gaactctggg aagtgttttt atcgtggcgg gaaccacaat
     8461 tggcgcaggc atgctggcaa tgccgctggc tgcggcaggt gttggtttta gcgtcacgtt
     8521 aatcttgttg attgggcttt gggcgttgat gtgctacacg gcgctattac tgctggaggt
     8581 gtaccagcat gttccggcag ataccggtct gggcacgctg gcaaaacgct atctgggacg
     8641 ctacggtcaa tggctgacgg gcttcagtat gatgttctta atgtatgctc tgactgcggc
     8701 atacatcagc ggtgccggtg aattgttggc ctccagcatc agcgactgga caggtatttc
     8761 aatgtcggca accgctggcg tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt
     8821 tggaacatcg ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa ttatttttct
     8881 ggtggtgatg ctggtattgc tgctaccgca tattcacaaa gtgaatcttt taaccctgcc
     8941 gttgcaacag gggctggctc tgtcagcaat cccggtgatt tttacctcat ttggttttca
     9001 cggtagcgtg ccgagtattg tcagctatat ggatggcaac gttcgtaagc tacgcagggt
     9061 gtttataacc ggtagtgcga tccccctggt ggcatatatt ttctggcagg tggcaacgct
     9121 tggcagcatt gattcaacaa cctttatggg attgctggct aatcatgctg gattaaacgg
     9181 gctgttacag gcgttacgcg aaatggtggc ctctccgcat gttgagctgg cagtgcattt
     9241 atttgctgat ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct tatttgatta
     9301 tctggctgat ttatttcagc gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat
     9361 tacctttctg ccgccgttgg cgtttgcact gttttatcca cgaggatttg tgatggcgct
     9421 gggttacgcc ggtgtggcgc tggcggtact ggcattgatt atcccttcgc tgttgacctg
     9481 gcaaagcaga aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct
     9541 ggtggtggtg tttctctgtg gtattgctgt gattggcgtg caatttttga ttgcggcagg
     9601 gttgttacca gaagtgaggt gatcagatag cctcaaattc cttattgggt gccagaatta
     9661 acgctgacac ccaatttggc ctcttaatgc aggcagcact gcttaaattt cttaccacta
     9721 ccgcacgggc aaggatcgtt acgccccggt ttctcttctg ctttgatcgg ttgctgaaca
     9781 gctttttcct gcggatgcgc catccagtac gcatgtagat caagcgccgc cagtcgaatg
     9841 gcatctacgc tctcttcaaa cgcttctggc gacatctttt ctacccgctc gaagttttcc
     9901 tcagtaccgt gcagcgcaat cgcctccagc gctggtttta acgaatcggg caacgttgac
     9961 cagtcagaaa gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc
    10021 tcgctgccat caacttctcg caagccgaat aacggctcaa actgctccgg gaattcgttc
    10081 agacgctctg cggtatcggc catatgttga aaagccaggt tcataaagcg cgtcatctct
    10141 ttctctgacg cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt
    10201 tccggttcaa tctcttgcgg agaactcaac accgccgtca ataaatcgtc cagctccgcc
    10261 acatcaagga tggcgtggtt agtgttgtat ttggtcagaa tatcgtccag ccattccaac
    10321 tcactttcgt ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag
    10381 cacctatctt acatgccggt ccgtatcaga gatacttttt gagtggcttt gccggtgatt
    10441 aaaaattaag gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt
    10501 tcacgacact gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg
    10561 acttgaaccc gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc
    10621 accatccggg ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga
    10681 tttgcaatcc gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca
    10741 gacatccgct tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg
    10801 accccgacct tggcaaggtc gtgctctacc aactgagcta ttcccgcatt cattaagcaa
    10861 tcagttaatc acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc
    10921 attctactta cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg
    10981 aaaattacgc cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg
    11041 caacattgac cacagagtca gcaccgcagc cacaaagaaa agtgcaatac cggcgtactc
    11101 aacccaaatg ttcggacgcc acagcagcca ggccaacgcc accatctggg cagtggtttt
    11161 cactttccca atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc
    11221 gcgtagcgca gaaataataa tttcacgggc gatcatcgtt gccgccggta gggtcaccca
    11281 ccagctgtga taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc
    11341 tacagggtca aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc
    11401 atcgaaccag tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca
    11461 ggtgacaggc agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt
    11521 aagcaacgta gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt
    11581 acccctatgt tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga
    11641 ccttgcgaaa tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc
    11701 aaaccgccca tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt
    11761 tccagggaac tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga
    11821 tcatgtgatt catcgcgaat atgctggata acatgcagcg cgggtgaatc tggcggcaaa
    11881 ctaaatccct catcttccgg ctcaaagaac agcgtttcca gtccagcctt acgatctgct
    11941 cctttggcaa cgccaagtag cagcggatga tttttatccc atgagacatc cagttcggcg
    12001 aagacatttt tcgcctgcgc aagctggcct ttgccgccgt cgataaggat cacatccggg
    12061 atcttactgt cgtcaatggc tttaccataa cgccgacgca gcacctgatt catcgccgca
    12121 taatcatcgc ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc acgcagcggg
    12181 ccgttagcat caaacaccac acaggaagcg acggtttgtt cgcccatggt atggctgatg
    12241 tcaaagcact ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt
    12301 cgctggtgaa cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg
    12361 cgcgcgagtt tcagataacg cgccctatcg ccgcgaggtt tggtttgaac attaatcttg
    12421 cgtcccgcca gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg
    12481 agcaggatct cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctgacctacg
    12541 aaggtttcta ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg
    12601 ctgccgagca ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg
    12661 aacgccacac caataacgtc aaggtcgtcg ccggtattgg aaacaaactg tttttcggtg
    12721 acgcgtcgca ccgcctgaat ttggtcgcga atacgtgccg cttcttcaaa ctccagattc
    12781 tgactggcag tttccatacg gctaatgagt tgcgtaagca cctgatcatc tttgccagac
    12841 aaaaacaggc gcacatactc gacctgctga gcgtattctt cttcactcac cagtccttca
    12901 acgcacggtc ccagacagcg ccctatctga tattgcagac acggacgcga gcgattgcga
    12961 taaacactat tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc cagagtttca
    13021 cgtacggcat agccattcgg gaacgggccg aaatattcac ctttggcatg cttcgcacca
    13081 cgatgcatcg ccagacgcgg gtgggtatcg ccactcagga agataaaagg ataagattta
    13141 tcatcgcgta gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc
    13201 aacagcgctt cggtttctgt atgagtaacc gttacatcaa tttgctgaat ctgggcgacc
    13261 agcgcttcgg ttttgcgcga agcgaggttg ctacggaaat agctggaaag ccgttttttc
    13321 aggtctttcg ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg
    13381 cctggctggc tggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac
    13441 ttgataatgt ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat
    13501 cgccatgaat gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac
    13561 tgagattgag ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa
    13621 tctgcaattc acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct
    13681 ggcttaacgc catttgttga gcgatgtcag aagcaatgta acgctgccct gaatagacag
    13741 aacgaatcgc actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac
    13801 cggcctgcat gactttcgct ggtaaagggt tttctgtatg aacggtaagc atgatgattt
    13861 tgacatcagc tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca
    13921 tactcatgtc cattagcacc acgtcaacgg catttgtccg gcaccactta acggcgtctt
    13981 caccgcacga tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta
    14041 tccctgcgcg caccagttcg tggtcatcaa caagtagaac gttgatcaaa gaaatatctc
    14101 cagaaatagg gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca
    14161 actttgaaac gttaaaaatg gtgggtattc gatttttctc tcgtttttgt caattcgatt
    14221 gtccacagtt atggtcacgc ccgtcaccaa cggcttaccg gatcgtttca cagtaagccc
    14281 tacgatgaaa tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca gcccgctgaa
    14341 tatgtaacat taattaaccc gaacaatacg ggcaaaaaca ttcatgataa aaatatttat
    14401 tggtcattat attaacgtat tttatagcac tgccgatatc acgctcaaaa aacaaccact
    14461 gctattttta gcaaagctta tggtatactc cgccgcctta acatttttca ccgcaaattt
    14521 tcattgcaac atgacgagga aaataaatga gtacgcctga tttttctact gccgagaata
    14581 atcaagaact ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg atgctgcagg
    14641 cgatgggaca agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga
    14701 tcgaagacga aacccaggct gcagtatttt ccaaaacggt taagcaaatt aaacaagcct
    14761 accgtcagta atataaaacc ggctgagagc gtgcctttca gccggttttt gcatctggca
    14821 cgcagaaaga aaagcgatca aattaagcca gtagcggccg cgtaacaggc cacctgggtc
    14881 ttatttggtg cattgatttt tttctgcatg tttttctgat ggaaattgac cgtattctca
    14941 gagattgaca aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccatttcaga
    15001 atttcttttt cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa
    15061 cgcatcagag ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca
    15121 ctaagaatgg gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga
    15181 tttggcagca ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc
    15241 cataatggct gtgcttcgct gaataagtca tcattccaca ttaaatggcc ctgactaaag
    15301 ttttcagggt tcagcacagg atcaattgcg agaaaatttt ttgcctgata ataactaacc
    15361 cacgactcag ggtaattggt gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg
    15421 cgaacacata acgaatagta atcgtactcc agctgctgag cctgaagctc tatttcatgg
    15481 tagacctctt ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg acgccaactg
    15541 aaaaaatccg tatcctgcat agtaaaccgc aacgcccctg agagtgagta tcatttataa
    15601 tgataattca aactatcaca taaataagat ttatatataa tttatattat tcaggcaatg
    15661 aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc tgatttgtta
    15721 agcagtttta tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat
    15781 tttttcttat tattgcagca gaaacttctc gaggaactgt cgggtgcgag gctgctgggg
    15841 atcggcaaat aacgcttttg ctgccccctg ctcgactatc cgccccaggt ccataaaaat
    15901 cgcccggtcc gcaacatccc gggcaaagct catttcgtgc gtcacaatca ccatcgtgcg
    15961 cttttcctgc gccagctggc ggattgtgtt caggacttcg cccaccagct ctggatccag
    16021 cgctgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagcg cacgcgcaat
    16081 cgctacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt ctttaccggc
    16141 cagcccaact ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc
    16201 tttgacgatc accggccctt caataatgtt ttccagcacc gtacgatgcg gaaacaaatt
    16261 aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg atttttgctg
    16321 acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc ccgcttccgg
    16381 ttgttccagc agatttatgc tgcgtagcaa cgtggttttg ccggaaccac tcggaccgat
    16441 aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca ccgtctgacc
    16501 ttggaatttt ttcaccaggt tcttaacttc aatggcactc atttgggttc tctctcctgg
    16561 cgattaagtt gattctcaaa atggttctgc aacgtcgata acacagtcgc catgatccag
    16621 tagatgagcg aagccgccag gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac
    16681 tgcgcctgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt atcttttacc
    16741 agactgataa agctgttcga cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc
    16801 gcgcgacgca tggtctgcca cggcgtcata ccaatactgg cagcggcttc ccactgacct
    16861 ttatcaatag aagaaatggc ggcgcgcagc gtttcggcgg cataggcggc agtgttcagc
    16921 gacagaccaa tcattgctga cagaatggga tctaattcga taccaaactg cggcaggccg
    16981 taatagatca taaacagttg tgcgatgagc ggcgtaccac gaaaaataga gatataaaaa
    17041 cgcgccagcc agcgcacggg ccagattggc gacaggcgca tcagcgcgag aataaacccg
    17101 agtagtaagc caaaaaacat gccgccgata ctgagttgca gggtataccc agcccctttc
    17161 aacaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag cgttcccgga
    17221 gctaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga ataaacagaa
    17281 tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagcccc gccatcgctt
    17341 ttccggtata cacaggatca agcagaatgc cttccagacg cgccagcaat ttcactgctt
    17401 ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc cagagtaaaa
    17461 tttccgctga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt
    17521 tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca
    17581 tcaggtgttc cagcccaaca gccaatccgg cgtgagttcc ggcactgccc gatgcgacca
    17641 ccaccgacga aatattaacc gccccttcac actgttgcgc gatttccagc gcactctcca
    17701 cataacccag cgcgcccaga gcattagaac cgccaaccgg aatgacatac gggcgaaagc
    17761 cttgtgcttc gactcgcgtc gccagctctt ccagttgggc attgggatcg gtcagtgcgt
    17821 cgcacatttc aatctgggta ttgaacagat tcagcaacaa acgattgccg ttggttaaat
    17881 agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc aggccgagtt
    17941 tcgccgcaac tgcggcagtc tgccgcacat ggttagactg gatcgcgccg gcagtaatca
    18001 gcgtatcggc accttcgcgc agggcatctg ccgcgagaaa ttccagctta cgtaatttat
    18061 tgccgcccat tgccatgggt gtgacgtcat cccgtttgat gaaaatttcc cgtccaagat
    18121 aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac tccagccgtg
    18181 gaaaacgggt taaattatgc agtggcataa tagcctccga tgtgtgttgt tgtgattttc
    18241 ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg cccttttttg
    18301 tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa gggcttgcag
    18361 agtgccatct ttttgcattt ccgcaattgc atcgttcact gctttcagca ggtcttcatt
    18421 tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg ttactgccag
    18481 cgtatcgttg gttttcttca ccagatccag cgccgccaga cggtcaacga ggatcgcatc
    18541 gatacggcct acgcgcagat cctgatattt ggtcgggtca tcatcatagg tacgcacatc
    18601 gacgccctga acattctgcc gcagccactc ttcatagttg gtgcccagac cgacacccac
    18661 ttttttgcct ttcagatcag cagccgtttt aatggtgcct tcgttacctt ttttcaccag
    18721 cgcctgaata ccagaaatgg tgtacggggt agagaaatcg tattttttct tgcgctcatc
    18781 agaaatggtg acctgattaa tcaccacatc aatacgttta gagtccagcg acgctagcat
    18841 accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg ccagctgttg
    18901 ggcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac tgaacggcgg
    18961 ataagttcct tccagcccta ccagtagcgt accgcgctct ttgactttat taagcagacc
    19021 ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc gccacggcca tcacacccat
    19081 caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg ttatgtctgt
    19141 ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc ttattcttat
    19201 ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg tacacgtttg
    19261 acgctgatag tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc tggtggtggg
    19321 cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga gggaaatatt
    19381 ttgctcatgt agatgattcc ccaaacggcg caaacgaacg acatattcac gcaccgtacc
    19441 tgggctcatt tcagtttgct caaaaagaaa ttgcttaaag ccgataatat cgaaaaaatc
    19501 actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc atgactgttg
    19561 ttccacttgc cagccatttt catcaagcag cgtgtccagg cgggaaatct cgattttatt
    19621 cactattttg ccgtcgcgaa ctaaggtaat gcgatcgagt aatttacggc aatgcgcgca
    19681 atgggcctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc ttttcaggtg
    19741 ctgcaccatc attaagaact cctggtagtc aaagttaaag tgcggcattt actgacgtta
    19801 taacttaccc agtttagtgc ttaaccgttt aatggcctgg ctgtgtaact gactgacccg
    19861 cgattccccg acctccagca ccgcgccaat ctctttgaga ttcagctctt cctggtaata
    19921 gagggttaat accagttttt cgcgctccgg caacgtttcg atagcttcca tcacccgctg
    19981 gcgcagatta ctgtccagca gttgttgtag cgggttttct cgctgatgat catcagtaac
    20041 cagttcgatg ctatcgccgt gctcttcgcg ccactcatcg taggagaaaa gctggctgtt
    20101 attggtgtcg agcaacattt ggcgataatc ggcaatatcg atccctaaac gttccgctac
    20161 ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg cctgtgccac
    20221 ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa gttcatccag
    20281 catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt gtagggcgtc
    20341 atagcgttcg acggcattaa gtaacccaat gccgcccgcc tgtagcagat cgtcaagttc
    20401 cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacgcacca gcgggacata
    20461 acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg aattcacgat
    20521 aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag ggtttttaat
    20581 cggacgatta gtgggtgaaa tgaggggtta tttgggggtt accggtaaat tccatgcaga
    20641 aaaaaccccg ccggtggcgg ggaagcacgt tgctgacaaa ttgcgcttta tgttgccgga
    20701 tgcggcgtaa acgccttatc cggcctacaa aaatgtgcaa attcaataaa ttgcaattca
    20761 ccttgtaggc ctgataagtg cagcgcatca ggcaatttgg cgttgccgtc agtctcagtt
    20821 aatcaggtta cgacgattaa ccctgcagca gagacagaac ctgctgcggt acctggttgg
    20881 cttttgccag cacggagtta ccggcctgct ggatgatctg cgctttcgac atgttggaca
    20941 cttcggtcgc atagtcggcg tcctgaatac gggactgcgc ttcagacagg ttggtagtgg
    21001 tgttgttcag gttggtgact gcagaatcca gacggttctg aatagcaccc agggatgaac
    21061 ggaatttgtc gatggagctg atagcttcat ccagggcagc cagaggatct ttggttgcag
    21121 tgcctgcact ggtggtttca gtggtcagtt tgcctgtaga gttaacataa gcctgtgccc
    21181 caacagtagc tgcaaattta ccagttgcac ctgcgccatt tgaagctaca gttactgtac
    21241 cggtatcctt atcgacgttg tagttgatag tgtaagatgc ggttgtggtg agctctttgt
    21301 ctgcatcagt ataggtgtca ttcgaattac cagcaccatt agtattcgca ccaccgaagg
    21361 acgccgttgc agactgaata cctgtattat acttaattgt cgcaccagta actgctgtgt
    21421 tagccgttgc agcactctgc actgtgctca gtacagtatc agcgcttaca gttttggtgt
    21481 aggttacgcc agaagttgcc gctgcaccaa agttataagt tgtgccagac aactggatag
    21541 aaccagttgt cgctaaggta ctaccgctag caaacagatc ggacaatttc gctgaagatg
    21601 caatgccggc gttgtttgta gataggttac cagtggcgtc caggtacgcg gctttaccac
    21661 cgatggtcac atcgccatta gcatctacat caaacttcac atcaccagta ctacgagtat
    21721 aaacacctga tgcagtagtg ccagccgctg gtttcagagt attcgcaaag ttaacaacat
    21781 cgccatctgc aactgttgct tgagtggtga agttcccttt agccgcatca taagtataga
    21841 tcgcagcact cgagccagta gtagtaactg tatctccggt tttcaggcga gacagtgcat
    21901 cgctagcgct gagtgctgta ttgtttgtgg tcacagcata aggacctgtt cccgttgcac
    21961 cagcagcggt cagatcgctg actgtagcag ctttgttcgc aatagtgcct ttgccattca
    22021 cgttaaaacc attcagcccc agcgtatctg agtcaatttt cttcagatca atcgtgatag
    22081 tctggccgtc attcgcacca acctgaattt tcattgaacc gtctttcgcc agtacgttca
    22141 cgccgttgaa ctgggtctgg ccagatacgc ggtcaatttc gtccagacgg gatttgattt
    22201 cgtcctgaat ggagtccaga tccgaatcgg agttagtccc ggtagaagcc tgaaccgtca
    22261 gttcaagaat acgctgtaag ttgttgttga tttcggacag cgcgccttca gtggtctgcg
    22321 caacggagat accgtcgttg gcgttacggg ccgcctgagt caggccttta atattagaag
    22381 taaaacggtt agcaatcgcc tgacctgcgg cgtcatcctt cgcgctgtta atacgcaagc
    22441 cagaagacag acgctcgata gaactcgaca gcgcagactg gttcttgttg atattatttt
    22501 gagtgatcag cgagaggctg ttggtattaa tgacttgtgc catgattcgt tatcctatat
    22561 tgcaagtcgt tgattacgta ttgggtttcc acccgtcggc tcaatcgccg tcaaccctgt
    22621 tatcgtctgt cgtaaaacaa cctttagaat ttttttcaca aacagccatt ttttgttagt
    22681 cgccgaaata ctcttttctc tgccccttat tcccgctatt aaaaaaaaca attaaacgta
    22741 aactttgcgc aattcaggcc aataaccccg gtattcgttt tacgtgtcga aagataaaag
    22801 gaaatcgcat ggcaagtatt tcatcgctgg gagtcgggtc aggtctggat ttaagttcaa
    22861 tccttgatag cctcaccgcc gcgcaaaaag cgacgctaac ccctatttca aatcagcaat
    22921 cgtcgtttac cgctaaactt agcgcctacg gtacgctgaa aagcgcgctg acgactttcc
    22981 agaccgccaa tactgcattg tctaaagccg atcttttttc cgccaccagc actaccagca
    23041 gcaccaccgc gttcagtgcc accactgcgg gtaacgccat cgccgggaaa tacaccatca
    23101 gcgtcaccca tctggcgcag gcgcaaaccc tgaccacgcg caccaccaga gatgatacga
    23161 aaacggcgat cgccaccagc gacagcaaac tcaccattca acaaggcggc gacaaagatc
    23221 cgattaccat tgatatcagc gcggctaact catcgttaag cgggatccgt gatgctatca
    23281 acaacgcaaa agcgggcgta agtgcgagca tcattaacgt gggtaacggt gaatatcgtc
    23341 tgtcagtcac atcaaatgac accggccttg ataatgcgat gacactctcg gtcagcggtg
    23401 atgatgcgct acaaagtttt atgggctatg acgccagtgc cagcagcaac ggtatggagg
    23461 tctcggtcgc cgccaagaat gcgcagctga cggtcaacaa cgtcgccatt gagaacagca
    23521 gcaacaccat cagcgacgcg ctggaaaaca tcaccctgaa cctgaacgat gtcaccacgg
    23581 gcaaccagac gctaaccatc actcaggaca cctccaaagc gcaaacggcg attaaagact
    23641 gggtgaatgc ctataactcg ttaatagata ccttcagtag cctgaccaaa tacaccgccg
    23701 tagatgcggg agctgatagc cagagttcta gcaatggcgc actgctcggc gactccacgc
    23761 tgcggacgat tcagacgcag ttgaagtcga tgctgagtaa taccgtcagt tcttccagct
    23821 ataaaacgct ggcgcagatt ggtatcacga ccgatcccag cgatggcaaa ctggaactgg
    23881 atgccgacaa actcaccgct gcactgaaaa aagatgccag cggcgtaggt gcattgattg
    23941 ttggcgatgg taaaaaaacc ggcatcacga ccaccatcgg cagcaacctg accagttggc
    24001 tttcgacaac gggcattatt aaagccgcta ccgatggcgt tagtaagacc ctgaataagt
    24061 taactaaaga ctacaacgcc gccagcgatc gcattgatgc gcaggtcgcg cgctacaaaa
    24121 aacaatttac ccaactggac gttttaatga cctcgttaaa cagcaccagc agctacttaa
    24181 cgcagcagtt cgaaaacaac agtaattcca agtaatcatt attcatcggg agacaggtca
    24241 tgtacgcggc aaaaggcacc caggcctatg cacaaattgg cgtcgaaagc gccgtaatga
    24301 gcgccagcca gcagcagctg gtcaccatgc tatttgatgg agtgctgagc gcactggtta
    24361 gagcgcgcct gtttatgcag gacaacaatc agcaaggcaa aggcgtctct ttgtcaaaag
    24421 cgatcaacat cattgagaac ggactgcggg tgagtcttga tgaagagagc aaagacgaac
    24481 taacccaaaa cttgattgct ctttatagct atatggtcag gcgcttgctg caagccaatt
    24541 tacgcaacga tgtctccgca gtcgaagaag tggaagcatt aatgcgcaat attgccgatg
    24601 cctggaaaga gtcgttactc tccccttctt tgattcagga cccagtctga tgaaccatgc
    24661 accgcatttg tatttcgcct ggcaacaact cgtcgaaaaa agccagctca tgttacgcct
    24721 ggcaacggaa gaacaatggg acgaactcat cgccagcgaa atggcgtatg tgaatgcggt
    24781 gcaggagtat agcacacctg actgaagagg ttgccccgtc caccacgatg caggagcagc
    24841 tccgcccgat gctgcgcctg attctcgaca acgaaagcaa ggtaaagcag ttattacaga
    24901 ttcgtatgga tgaactggcg aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa
    24961 gtgcctatgg cgatcagggc ggctttgtgc tggctccgca agataacttc tcgtgaatct
    25021 taacgagcta atggttcgcg aataatccga ttacggctac gcttctaatg ttccccttga
    25081 atggagtcga agaatgcgta atcccacgct gttacaatgt tttcactggt attacccgga
    25141 aggcggtaag ctctggcctg aactggccga gcgcgccgac ggttttaatg atattggtat
    25201 caatatggtc tggttgccgc ccgcctataa aggcgcatcg ggcgggtatt cggtcggcta
    25261 cgactcctat aatttatttg atttaggcga gtttgatcag aaaggcagca tccctactaa
    25321 atatggcgat aaagtacaat tgctggccgc cattgacgcc ctgaaacgta atgacattgc
    25381 ggtgctgttg gacgtggtag tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg
    25441 cgtgcagcgt gtaaatgctg acgaccgtac gcaaattgat gaagaaatca ttgagtgcga
    25501 aggctggacg cgttacacct tccccgcccg tgccgggcaa tactcacagt ttatctggga
    25561 tttcaaatgt tttagcggta tcgaccatat cgaaaaccct gatgaagatg gcatatttaa
    25621 aattgttaac gactacaccg gcgaaggctg gaacgatcag gttgatgatg aattaggtaa
    25681 tttcgattat ctgatgggcg agaatatcga ttttcgcaat catgccgtga cggaagagat
    25741 taaatactgg gcgcgctggg tgatggaaca aacgcaatgc gacggttttc gtcttgatgc
    25801 ggtcaaacat attccggcgt ggttttataa agagtggatc gaacacgtac aggaagttgc
    25861 gccaaagccg ctgtttattg tggcggagta ctggtcgcat gaagtcgata aactgcaaac
    25921 gtatattgat caggtggaag gcaaaaccat gctgttcgat gcgccgctgc agatgaaatt
    25981 ccatgaagca tcgcgcatgg ggcgcgacta cgacatgacg cagattttca cgggtacatt
    26041 agtggaagcc gatcctttcc acgccgtgac gctcgttgcc aatcacgaca cccaaccgtt
    26101 gcaagccctc gaagcgccgg tcgaaccgtg gtttaaaccg ctggcgtatg ccttaatttt
    26161 gttgcgggaa aatggcgttc cttcggtatt ctatccggac ctctacggtg cgcattacga
    26221 agatgtcggt ggtgacgggc aaacctatcc gatagatatg ccaataatcg aacagcttga
    26281 tgagttaatt ctcgcccgtc agcgtttcgc ccacggtgta cagacgttat ttttcgacca
    26341 tccgaactgc attgccttta gccgcagtgg caccgacgaa tatcccggct gcgtggtggt
    26401 catgtcgaac ggggatgatg gcgaaaaaac cattcatctg ggagagaatt acggcaataa
    26461 aacctggcgt gattttctgg ggaaccggca agagagcgta ataaccgacg aacacggcga
    26521 agcaaccttc ttttgcaacg gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta
    26581 aattcatccc cggcggcaag ccggggagat ttcattacgg cagtggcgtc ggtaacggcg
    26641 ctttatccag cgcggcggca cactctgccg tcgggcgatc cacacgctcc atcgtcatac
    26701 catcgtactc aatggtgtta ccgtcacgct caacctcgta cagctcacgt tttacggtaa
    26761 cgttagtgag atcatcagac atcaatgcca acttacccgg cacagcaatc acccgctgcc
    26821 actgacggca atccagagta tcaccctctt tagtgacaat caggctgcct atcgcttccg
    26881 gactgaccag cgcacgttgc ggccctttgg tctgccagta gcccgccagc cagtccggcg
    26941 cgggggtttt cacgacattg ttatagtttt cgacttcggc acagcccgcc agcaacatga
    27001 gcgcgcctgc aattgcgagt tttttcatca ttcgtcctgc atgagaagaa aagaggattg
    27061 tggcattaaa gccctgatgt cgccagcctt tccaggttca gagagaaatt tgatctaact
    27121 attatttttg tgaattatta ccccgaaaat aaatttgcat gatgtatgat tcgcgactct
    27181 tgtttcaaac cttctgagtt cagaggctac attcatgtca tggcagcaat tcaaacacgc
    27241 ctggttgatt aaattctggg cacccatccc tgcggtcatc gcggcgggta ttctctccac
    27301 ttactatttt ggcattactg gcaccttttg ggctgtcacg ggtgaattta cccgttgggg
    27361 cggccagctc ctgcagctgt ttggcgtcca tgctgaagag tggggttatt ttaaaattat
    27421 ccatctggaa ggatcgccat taacccgcat cgacgggatg atgatcctcg gcatgtttgg
    27481 cggctgcttt gccgcagcgc tgtgggccaa caatgtcaaa ctgcgcatgc cacgcagccg
    27541 tatccgcatt atgcaggcca ttattggcgg tattatcgct ggttttggcg cgcgtctggc
    27601 aatgggctgt aacctggcgg cgttctttac cggtattcct cagttctcgc tgcatgcctg
    27661 gttctttgcc atcgccactg ccattggttc atggtttggc gcacgcttta ccctgctgcc
    27721 catcttccgt attcccgtga aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa
    27781 accggatcag gcgcggcgtc gttttcgtct cgggatgctg gtctttttcg gtatgctggg
    27841 ctgggcgctg ctcacggcga tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt
    27901 cggctttggt ttactgattg aacgtgcgca aatctgcttt acttcggcgt tccgcgatat
    27961 gtggatcacc ggacgtaccc atatggcgaa agcaatcatt atcggtatgg cggtgagtgc
    28021 catcgggatc ttcagttacg tacagttagg cgttgaaccc aaaatcatgt gggcgggacc
    28081 aaacgcggta attggtggtt tactgtttgg ttttggcatc gtgctggcag gcggctgcga
    28141 aaccggctgg atgtaccgcg cggtagaagg ccaggtgcac tactggtggg tcggtctggg
    28201 caatgtgatc ggctcgacga ttctggcgta ttactgggat gatttcgctc cggcgctggc
    28261 caccgactgg gacaaaatca acctgctgaa aacctttggc ccgatgggtg gcctgctggt
    28321 gacatatttg ctgttgttta ctgcgctgat gttgattatc ggctgggaaa aacgcttctt
    28381 ccgccgtgcg gcaccgcaga ctgctaagga gatcgcatga aaaatatcgt tcctgattac
    28441 cgccttgata tggtgggtga accctgccct tatccggcag tcgcaaccct tgaggcgatg
    28501 ccgcagttaa aaaaagggga aatcctggaa gtggtgagcg actgtccaca gtcgatcaat
    28561 aatattccac tggatgcgcg taatcacggc tatacggtgc gggatattca gcaagacggg
    28621 ccgaccattc gttatttaat tcagaagtaa tccctcattc ccgctgtggc ccacagcggg
    28681 aacatcgttg cctctgacac atagatttta cccgccccct ctcctattct tttcagtatg
    28741 atctcaggag gtttctatgt gtggacgctt tgcccaatcc caaacgcgtg aagattacct
    28801 tgcgcttctc gcggaagata ttgaacgcga tattccctac gatcccgaac ccattggcag
    28861 atacaacgtc gcgccgggaa ccaaagttct tctgctcagt gaacgtgatg aacaccttca
    28921 tctggatccg gttttctggg gatatgctcc cggatggtgg gataaaccgc cgctgattaa
    28981 cgcccgcgta gaaactgccg caaccagtcg catgtttaaa ccgctctggc aacatggtcg
    29041 ggcaatctgt tttgccgatg gctggtttga gtggaaaaaa gaaggcgaca aaaaacagcc
    29101 tttttttatc tatcgtgctg atggacaacc tatttttatg gccgcgatag gtagcacacc
    29161 atttgagcat ggtgacgaag ccgaaggatt tttaattgtc actgctgcag cagaccaggg
    29221 tctggtagac attcatgacc gccgcccgct ggttctgtcg ccagaagctg cacaggaatg
    29281 gatgcggcaa gagattggcg gtaaagaagc atcagaaatt gctaccaacg gctgtgtgcc
    29341 agcaaaccag ttcacctggc acccggtatc gcgcgcggta ggtaatgtca aaaaccaggg
    29401 cgcggagtta attcaacctg tttgttgaca tcctccacgc cctgaaggac gaggctccct
    29461 gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gggcggcctg
    29521 actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcgc
    29581 catttacatc ggcgttcgct gtatatccac atacctggca tctgaattta ctttgtgaca
    29641 ggcggttttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg cacgctggcg
    29701 gaacagcaaa cacctg
//
LOCUS       DAJTYD010000033        28589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTYD010000033 DAJTYD010000000
VERSION     DAJTYD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.033"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1176
                     /gene="potE"
                     /locus_tag="OO585_001330"
     CDS             <1..1176
                     /gene="potE"
                     /locus_tag="OO585_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6628975.1"
                     /translation="AVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTY
                     GVSLLIANVAIAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQIS
                     SITVWGVIIPVVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLES
                     ACANAEVVENPERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFA
                     QMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQ
                     GMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPP
                     SKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPCFELKNKHG"
     gene            1252..1401
                     /locus_tag="OO585_001331"
                     /pseudo
     CDS             1252..1401
                     /locus_tag="OO585_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1525..2175
                     /locus_tag="OO585_001332"
     CDS             1525..2175
                     /locus_tag="OO585_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCR6628976.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(2216..2710)
                     /locus_tag="OO585_001333"
     CDS             complement(2216..2710)
                     /locus_tag="OO585_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628977.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(2925..4565)
                     /gene="pgm"
                     /locus_tag="OO585_001334"
     CDS             complement(2925..4565)
                     /gene="pgm"
                     /locus_tag="OO585_001334"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6628978.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMEAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4591..5136)
                     /gene="seqA"
                     /locus_tag="OO585_001335"
     CDS             complement(4591..5136)
                     /gene="seqA"
                     /locus_tag="OO585_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6628979.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5321..6085
                     /gene="ybfF"
                     /locus_tag="OO585_001336"
     CDS             5321..6085
                     /gene="ybfF"
                     /locus_tag="OO585_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6628980.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6225..6518
                     /gene="ybfE"
                     /locus_tag="OO585_001337"
     CDS             6225..6518
                     /gene="ybfE"
                     /locus_tag="OO585_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6628981.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6658..7188
                     /gene="fldA"
                     /locus_tag="OO585_001338"
     CDS             6658..7188
                     /gene="fldA"
                     /locus_tag="OO585_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6628982.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7398..7484
                     /gene="uof"
                     /locus_tag="OO585_001339"
     CDS             7398..7484
                     /gene="uof"
                     /locus_tag="OO585_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6628983.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7477..7923
                     /gene="fur"
                     /locus_tag="OO585_001340"
     CDS             7477..7923
                     /gene="fur"
                     /locus_tag="OO585_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6628984.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8008..8334)
                     /locus_tag="OO585_001341"
     CDS             complement(8008..8334)
                     /locus_tag="OO585_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6628985.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFPDQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8384..9790)
                     /gene="chiP"
                     /locus_tag="OO585_001342"
     CDS             complement(8384..9790)
                     /gene="chiP"
                     /locus_tag="OO585_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6628986.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10237..11901)
                     /gene="glnS"
                     /locus_tag="OO585_001343"
     CDS             complement(10237..11901)
                     /gene="glnS"
                     /locus_tag="OO585_001343"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6628987.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12105..14051)
                     /gene="nagE"
                     /locus_tag="OO585_001344"
     CDS             complement(12105..14051)
                     /gene="nagE"
                     /locus_tag="OO585_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6628988.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14384..15184
                     /gene="nagB"
                     /locus_tag="OO585_001345"
     CDS             14384..15184
                     /gene="nagB"
                     /locus_tag="OO585_001345"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6628989.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15244..16392
                     /gene="nagA"
                     /locus_tag="OO585_001346"
     CDS             15244..16392
                     /gene="nagA"
                     /locus_tag="OO585_001346"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6628990.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16401..17621
                     /gene="nagC"
                     /locus_tag="OO585_001347"
                     /gene_synonym="nagR"
     CDS             16401..17621
                     /gene="nagC"
                     /locus_tag="OO585_001347"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6628991.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYIGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            17669..18421
                     /gene="nagD"
                     /locus_tag="OO585_001348"
     CDS             17669..18421
                     /gene="nagD"
                     /locus_tag="OO585_001348"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6628992.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            18818..20482
                     /gene="asnB"
                     /locus_tag="OO585_001349"
     CDS             18818..20482
                     /gene="asnB"
                     /locus_tag="OO585_001349"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6628993.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            20863..20939
                     /locus_tag="OO585_001350"
     tRNA            20863..20939
                     /locus_tag="OO585_001350"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20897..20899,aa:Met,seq:cat)
     gene            20948..21032
                     /locus_tag="OO585_001351"
     tRNA            20948..21032
                     /locus_tag="OO585_001351"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20982..20984,aa:Leu,seq:tag)
     gene            21056..21130
                     /locus_tag="OO585_001352"
     tRNA            21056..21130
                     /locus_tag="OO585_001352"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21088..21090,aa:Gln,seq:ttg)
     gene            21165..21239
                     /locus_tag="OO585_001353"
     tRNA            21165..21239
                     /locus_tag="OO585_001353"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21197..21199,aa:Gln,seq:ttg)
     gene            21255..21331
                     /locus_tag="OO585_001354"
     tRNA            21255..21331
                     /locus_tag="OO585_001354"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21289..21291,aa:Met,seq:cat)
     gene            21380..21454
                     /locus_tag="OO585_001355"
     tRNA            21380..21454
                     /locus_tag="OO585_001355"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21412..21414,aa:Gln,seq:ctg)
     gene            21492..21566
                     /locus_tag="OO585_001356"
     tRNA            21492..21566
                     /locus_tag="OO585_001356"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21524..21526,aa:Gln,seq:ctg)
     gene            complement(21720..22895)
                     /gene="ubiF"
                     /locus_tag="OO585_001357"
     CDS             complement(21720..22895)
                     /gene="ubiF"
                     /locus_tag="OO585_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6628994.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23041..24465
                     /gene="miaB"
                     /locus_tag="OO585_001358"
     CDS             23041..24465
                     /gene="miaB"
                     /locus_tag="OO585_001358"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6628995.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPSETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            24618..25658
                     /locus_tag="OO585_001359"
     CDS             24618..25658
                     /locus_tag="OO585_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6628996.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            25655..26122
                     /gene="ybeY"
                     /locus_tag="OO585_001360"
     CDS             25655..26122
                     /gene="ybeY"
                     /locus_tag="OO585_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6628997.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26212..27090
                     /gene="corC"
                     /locus_tag="OO585_001361"
     CDS             26212..27090
                     /gene="corC"
                     /locus_tag="OO585_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6628998.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27115..>28589
                     /gene="lnt"
                     /locus_tag="OO585_001362"
     CDS             27115..>28589
                     /gene="lnt"
                     /locus_tag="OO585_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6628999.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTHEVLTTNVTPTTGLTPYARTGNWPL"
BASE COUNT         6981 a         7340 c         7285 g         6983 t
ORIGIN      
        1 gccgtaggct caatggcact ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa
       61 tcaggcggta tgggcggtta tgcggagtat gctttcggta agtccggcaa ttttatggcg
      121 aactacacct acggtgtctc gctgctgatt gctaacgtcg cgattgctat ttcggcggtt
      181 ggttacggca ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc
      241 atcggagtgc tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa
      301 atcagtagca ttactgtgtg gggagtcatt attccggtcg tcggtctgtg cattattggc
      361 tggttctggt ttagtccgac gctgtacgct aactcatgga acccgcacca tgtgccgttc
      421 tttagcgctg taggttcttc catcgccatg acgttgtggg ctttcctcgg tctggagtca
      481 gcctgtgcca acgccgaagt agtggaaaac ccggaacgta acgtgccgat tgcagttctt
      541 ggcggtactt taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgccgggatt
      601 gtgcctaata tggagctggc aaattcaacg gcaccatttg gcctggcttt cgcacagatg
      661 ttcacaccag aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttca
      721 ctgcttggct ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac
      781 ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc
      841 attgtgatta ttcagagtgg attggcactg atgaccatta gcccgtcact gaacagccag
      901 ttcaacgtgc tggttaacct ggccgtggtg accaatatca ttccgtatat tctgtcaatg
      961 gcggcattag tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagtagca
     1021 aactttgttg cctttgttgg tgcgatgtac agtttctatg cgctgtactc atccggggaa
     1081 gaagccatgc tgtacggttc tatcgtgacc ttccttggtt ggacactgta tggtctggta
     1141 tcaccatgct ttgaactgaa aaataaacac ggttaataaa acaaagtaaa aaagggcgat
     1201 tgaatgatcg ccctttctgt ttgttggcaa atagttcgtt atttatgccg catccggcag
     1261 atgtagggtc taaacgcgat cgaacacagc aactatttgc gtaattatat ggggcatctg
     1321 cgacaaaaac tggaacagga tccggcccgg cctcgccatt ttattactga aaccggtatt
     1381 gggtatcggt ttatgctttg aaaaaagtgg caatgtatgg ataaagtcat tttttattca
     1441 aatataagga acttaatatt taaaaaatgt tccatacaat tcctcatcat aaatgacacc
     1501 gtagcacaca aggagcaagt agcgatgcgt gaaatgaatc agttagtaaa tactgaagat
     1561 tcagcctggc caataatcca gaactggtta aaagacgcca caaaccatac cgaactctta
     1621 ccggttaata aaaatttagc cgaaaccgca ctttatcaat tacaggtgac aacaaaatca
     1681 ccgatgggtg cgcttgtcta tggctccgga ggtctgctga ttgataatgg ttggttacgc
     1741 atagcaggat ctggtcaccc ccggttgcca cgagatccag taagttggac acaacgccct
     1801 gaattcgcag gcgtgcgggc tttacccatc gctgacgatg tcgcaggggg aatttttgct
     1861 cttaatggtg gcgatcttgg ggaagatact ggctgcgttt attattttgc gccagatacg
     1921 ctgaattggg aaagtctgga agttggatat tctgagtttc ttcagtgggc attatccggt
     1981 gatttagata ccttctatga aaatgtacga tggcagcaat ggcgagaaga cgtaataaaa
     2041 ttatcggcaa cggaagcctt cacgttctac cctttcttat gggtacaaag tgaagaagca
     2101 agaacaagaa aagttatttc gcttactgaa ctctgggaaa tgcagtatca aatgaaagaa
     2161 acattcacgc aataagacat gttcaggtcc gggtttcatt cccggactca ctattttaat
     2221 gtgtatttat cgctttcaat tcttgctggc aattttctga ctggcagcgt cgtaactcaa
     2281 taatcccgac atgcatcatc gagccaaaaa atccgctttc gctgacataa tcctcattga
     2341 ctatcggtcg agtgccgggc ttgccctggc aaacccgcat aaaaaatgag gtgtagcatt
     2401 gccaaccggg atttaaaccg accttttctt gtgctgacat attatgaaga taagtaagtt
     2461 ctttttcgtt cccgcctgac aactgccagc ggcgggtatt atcaggtaag ttatcaccac
     2521 ggcaatttgt cttacaccgg gaaggtgcaa tatttaccgc aatgctaccc gttaatcttc
     2581 tggttttatc tttaggggtc agtaaccagt cgccagctaa tgatgattgt tcaggttggg
     2641 cgcatgcagt cagcagtaat gtggcggtaa ttagtgacac tatgacgacc agactcctgt
     2701 tggtcttcat tgggatttcc ttgagctact cagtttgttg acaaagtgtt cgttgttttt
     2761 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc tcgtaaattc aatatattgc
     2821 accatcctcg taggcctgat aagcgtagcg catcaggcaa ttctgtgttt gtcatacgta
     2881 aaaaagggcg atcttccgac cgcccttttt cttatttaat gtgtttacgc gtttttcaga
     2941 acttcgctaa caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg
     3001 aagctttcgc agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag
     3061 ccgttgtcag tcatcacttt cagaccgccg atagaagcac cattgcccgg tgccgccgtc
     3121 aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc tcaccatttc cggagacagc
     3181 ttagacagcg ccgctttttg tgcggaagtc gcagctgcct gcaaacggtt gtagctcggc
     3241 gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgacagcg
     3301 gtgatttccg ccgccagcag acacatgatg atgccgtctt tgtcggtgga ccacggcgtg
     3361 ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc cgccaaagcc gaagctgccg
     3421 tcgaacagac catcaacaaa ccatttgaaa cctaccggga cttctaccag cttgcggccc
     3481 aagtcattga ccacacggtc gatcatcgca gaagaaacca gcgttttacc gacagcaaca
     3541 tctttgcccc actgcggacg atgctggaac aggtagttga tcgccaccgc caggtagtgg
     3601 ttcggattca tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta
     3661 gcaaacgcca gatcgaactt atcacgcagt gccagcaggc ccgccatcgc acactcggag
     3721 gagcagtcca tacggatcgc gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg
     3781 acctgatcgt taacgatggt caggttgagg ttgtaatact cgccaatacg cttccagtat
     3841 tcgataccgg aaccgcccag cggatcaacg cccagcgtca ggcccgcttt ctgaatcgcc
     3901 tccatatcaa cgatatcggc cagaccttcc acaaacggct gcaccagatc ctgctctttc
     3961 acatgaccgg atgccatcgc ttcgtcgagg gagatacgct tcacgccttt caggccatcg
     4021 gccagcagtg cgttggccct gtcttccacc actttggtga cgttggtatc agccgggcca
     4081 ccatttggcg gattgtactt gataccacca tcttccggcg ggttatggga cggtgtaatc
     4141 acgataccgt ctgccagcgg accacctttt ttattgtgaa ccaggatggc attggaaacg
     4201 gcaggcgttg gagtgaagcc attgttttcc tgcacaatga catcaacgcc gttcgctgcc
     4261 agcacttcca gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc tttacccaca
     4321 tagcaagggc cagtgatgcc gtttttcgca cgttcttcag caattgcctg agcgatcgcc
     4381 agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg
     4441 aatttcaccg cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc
     4501 agttgggcga cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt
     4561 gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt ccgcaaacct tctcaatcaa
     4621 ttccgccggg aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt
     4681 gttggtgttg gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt
     4741 accatttttc agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa
     4801 cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag
     4861 cataaagcga ttgaccgctc gcttttgctc tgcgtattcg tcagaaagca gaagttcacg
     4921 cattgcgcga accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga
     4981 cgcaacgcga acctctttcg tcaccggagc agcaggctgt gatgcggcgg aaaatttcaa
     5041 catacgccgt aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata
     5101 gctgtagagt tcatcatcaa cttcaatcgt tttcatctta atccagtgca gtgtcttagc
     5161 tgaatacaag tcgccaagag tatatgaata aatcccggca gcggatagcg tcaagcctgg
     5221 cttgcagaaa aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca
     5281 taatacccta acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc
     5341 gcaaactgca caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg
     5401 tagcctcgac aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca
     5461 ggttgatatg cgtaaccacg gtctttcacc gagagatccg gtaatgaatt acccggcgat
     5521 ggcgcaggat cttgttgata ctctggatgc acagcagatc gacaaagcaa catttatcgg
     5581 tcactccatg ggcggtaaag cggtaatggc acttacggca ctggcccgcg atcgcatcga
     5641 taaactggtg gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat
     5701 tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc
     5761 aataatgcgc cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt
     5821 tgacggggag tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg
     5881 ttgggagaaa atcccggcat gggatcaccc tgccctgttt atccctagcg gcaattctcc
     5941 gtatgttagc gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca
     6001 tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc
     6061 tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt
     6121 tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc
     6181 gggcagaggc ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa
     6241 cggaccgtac gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta
     6301 atccgctttc gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca
     6361 aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga
     6421 acgagctggc ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc
     6481 agcaactggc ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca
     6541 gtgtgcagtc ctgctcgttt gctgattccg aaagactgcc tgttctgcta tgattgcctt
     6601 tatccgtggg caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg
     6661 gctatcactg gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg
     6721 attcaaaaac agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa
     6781 gaagatctgg aagcttatga cattctgctg ctgggcatcc caacctggta ttatggcgaa
     6841 gcgcagtgtg actgggatga cttcttcccg actctcgaag agattgattt caacggcaaa
     6901 ctggttgcgc tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca
     6961 ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa cgatcgttgg tcactggcca
     7021 actgcgggct atcatttcga agcatcaaaa ggtctggctg atgacgacca ctttgtcggt
     7081 ctggctatcg acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt
     7141 aaacagattt ctgaagagtt gcatctcgac gaaattctca atgcctgatg tgatgcggcg
     7201 tagactcatg tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt
     7261 gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa
     7321 atgtaagctg tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta
     7381 ggcgtggcaa ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta
     7441 atgaagtgaa ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta
     7501 aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa tcctggaagt tcttcaggag
     7561 ccggacaacc atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa
     7621 gaaattggtc tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc
     7681 acccgccaca attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac
     7741 gatcacctga tctgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa
     7801 gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat
     7861 ctttacggtc actgtgccga aggcgattgc cgcgaagatg aacatgcgca cgaaggcaaa
     7921 taagccagcc tgaatgagaa aaagccaacc tgcgggttgg cttttttatg caagggaaat
     7981 aagaaaagcg agagttacag ctctcactta tttgttatta ctgcgaattt cctgccaaac
     8041 tttatcacaa tcggctttca ccgcctgatc atttccggtt tgcgttgcac gcagacactg
     8101 ctgataatcc agtacgcgga cactttcctg gtcgggaaaa tgctgatgtt gtttctcttt
     8161 cttcagcacg tttaacacgc tctggcaggc ttcaattttt tctggcgaac cttgtgcggt
     8221 gttgatacag gcgctgtatg cctctttcag acgactgtct tcctgtggcg cggttgattg
     8281 cgcacaagct accagccccg atgccattat ggcgatgaga atcagttttt tcatagcaaa
     8341 ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg
     8401 gtgcgattac cataaatttc acgtcacgct catcctggaa gatgttgccg taaccaccgc
     8461 cccagcttgg gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc
     8521 ctttggcgcg accgtcctga atggtgtaga ccgcatccag gctgtatgca gactcttcaa
     8581 tagtccggtt tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat
     8641 cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt
     8701 catacatcgc accgaagaag accgcttttt cgccgttagc gttgaagtcg gaacggttat
     8761 cccaccagat atccaggcgg ccgttggagg aagcgtaggc tggagtcata cgttgcagga
     8821 agtatccctg ctgaccgtca gccttaaccc aggtgccttc gaggcgcaaa tccactacgt
     8881 cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc ggtgccgtca taaaggtcgt
     8941 tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa ctggtagctg gtggttaacg
     9001 ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt
     9061 ccgcctgacc aaatgccgct tccagtacga agttattttt gaagtcgtat ttcgccccaa
     9121 gggagtgcag ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca
     9181 gatgccacgg cgctttgtat tcgttggtcc acatgtagga gaaactcaac gcaccagcat
     9241 cgccgtaatc aaaattcgcc ccggcttccg ccccctgata agtacctggc ataaagctcc
     9301 agtgcggcgc taacagcgtt tgaccagttg gctgaatgta acctgccctc gcccaaaccg
     9361 gaccgtattt aaatttggcc gcagctttat acaggcttat accgcttttg tcgccagacc
     9421 agtcttcgtc ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgggagctgt
     9481 cgccgttttc cgccatttca atcgccgtaa acacagcaat atcaaggccg aacatatcag
     9541 cagcataacc ggactgaaaa tcgaggttgg cattccaggt ggagtgagaa aggttggttt
     9601 tgtatttgtc gccgtcggta acatctttac ggtcgcgttc acgctgccag taatatatac
     9661 cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc attgccataa
     9721 agcccgacat tgctgtaaca ccggcgatag ccagcgccag cgtactacgt ttgccactaa
     9781 acgtacgcat gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga
     9841 aaaaaataaa aattaaaaaa gtagcattga gaaaagtctc gctgactaca ggaatagact
     9901 atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga acatgagcaa
     9961 ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa aggcttgcag
    10021 aagaataaaa gcgtaaattt ccagcaagat gaaagcggtt acattttatt taattatggt
    10081 ttgtgatttt aaatttattt tatggataaa ttgcgaagcg gaattaattc gcgaagaaaa
    10141 gcagaaaaaa cgccgcagag tgcggcgttt gggattgttt gctatttctt gccggatgcg
    10201 acgtaaatgc ctcacccggc ctggtagaac ttaaaattac tcgcctactt tcgcccaggt
    10261 atcacgcagc ccaacggtgc ggttaaatac cggtttttcc gctgtggaat ggcggctgtc
    10321 gaggcagaag taaccttcac gctcaaactg gaatgcttta cccgcaaccg catctttcag
    10381 cgacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa
    10441 atcatccgca gcacctgggt taggcacgct gaacagacgg tcgtacagac ggatttcaac
    10501 cggcagcgca tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc
    10561 cggatctttg cttaaggtgt cggcgtcata agtacagaag atggtggtga tattgccttc
    10621 ggcatctttc tccacgcgtt ctgccttaat gacgtaagca ttacgcagac gcacttcttt
    10681 acccagcacc agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcgat
    10741 ccaaatctca ctgctaaacg gcacctggcg gctacccatt tccggtttgt tcggatggtt
    10801 cggcatggta accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc
    10861 gataaccgcc attgcgcgcg gggcgttttc gttgagatct tcacggatgc aggattccag
    10921 cgacgccatc tcaatggtgt tgtcctgcct ggtcacgccg atgcgtttgc agaactcacg
    10981 aatagaagca gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc
    11041 atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct tggacatcac
    11101 agtgtattcc agattcaggc gcgagaattc gtactggcgc gggtgaacag gaatggtgat
    11161 gttgtccagc acccagtcgt acaggcgacg gttgtcctgg aactcaagcg tacacagaga
    11221 gtgcgtaata ccttccaggg catcgctgat gcagtgggtg aagtcgtaca tcgggtagat
    11281 gcaccacttg ttgccagtct ggtggtgttc agcaaattta atgcggtaca gcaccggatc
    11341 gcgcatcacg ataaacggtg aagccatgtc gattttcgca cgcaggcagg ctttaccttc
    11401 ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa cgctgcggtc
    11461 gcggtacggg ctgtttttac ccggttgcgt cagggtgccg cggtattcgc ggatctgttc
    11521 cggcgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg
    11581 gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca
    11641 ctctacgtcg tttttgatcg actcaacata ctcgatatct tcttttaccg ggttagtgtc
    11701 gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat
    11761 agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac
    11821 tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg
    11881 gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta
    11941 tgatcttata agcggaacag gctgacaact gttagttttt taaaattgat aatctaacaa
    12001 ggattatggg gatgattgat gaaaaaaaaa agcggcagag aagatctcca ccgcttgtca
    12061 ttgttggatg cgacgctcaa gcgtcgcatc aggcataaag cagattactt tttgatttca
    12121 tacagcggtg tttgacccgc cacaacatgg ccctgagctt taatgatcaa gccactgaaa
    12181 tcgtcgatat tgctgcaaac caccgggcta atcatcgagc gggcgttagc gttcaggtaa
    12241 tccagatcca tttccagaat cggttgccct gcgcttacct gcgcaccctc ttccaccaga
    12301 cgtttaaagc ctttaccttc cagcgctacg gtgtcgatac ccatatggac gacgatctcc
    12361 gcgccttttt cggtttccag gcagaacgcg tggttggtgt tgaagatttt cacgattgtc
    12421 cctgcggctg gtgatacgac gattttatct gtcggtttca ccgccacacc gtcacccacc
    12481 gctttgctgg cgaatgcttc gtcaggaacc tgatccagtg ccacgacatc accggtaatc
    12541 ggcgatacca gctccgcgat agataccgcg tttggtacag cctgcggttt tgctacaggc
    12601 gcggcagttg acggagttgc ttcagctgac gcagcggcta ccggaccacg ggcaacgact
    12661 ttcttcatcg catcgccgat ggattctgct ttcgcgccaa caatcacctg aatagtctgt
    12721 ttgttcagtt tcactacacc agaagcaccc agacgtttac acatcgtatc gttgacgcgg
    12781 gcagagtcag ccacggtcag gcgcagacgg gtgatacagg cgtcaattgc tttcaggttg
    12841 tcagtgccgc caaccgcagc aatatagttg gtcgccagtt gattgaggcc ttcttcagtg
    12901 ttgctgttgg cttcttcagt aacgatctca tcttctttat cttcacgacc cggcgttttc
    12961 aggttgaaca tgcggataac caaactgaac accacgaagt agatagcgaa gaagacaaca
    13021 cccatcacca gcagcatcca gacgttctgg ctggcggccg gcaggttata catcaacgcg
    13081 tagtcgatag cccccgcaga gaaagagaag ccagcatgga tacccagcag tgttgccaca
    13141 aacaggctga taccggtcag cagtgcgtgc aggaggtaca gcagcggtgc aaggaacatg
    13201 aacaggaatt ccagcggctc agtcacaccg gtcaggaacg cagtaacagc aacagaaagc
    13261 agcatcccgc caaccatcgg acgacgctct ttcggtgctg cgaagtacat cgccagcgcc
    13321 gcacccggca gaccgaacat cataatcggg aagaagccgg acatgaacat ccccgcggtg
    13381 ccgtcaccgg catagaagcg gttgatatca ccgtggaaaa ccgtacccgc cgcgttggtg
    13441 aattcaccaa tctggaacca ggcgatggtg ttcagcacct gatgcagacc ggttgggatc
    13501 agcagacggt tgatgaaacc aaagataccg gaacccagcg cgcccgcaga aacgatccac
    13561 tcgcctcctg catggatagc gtgctgtacc ggcggccaga cgtaaccaaa aatggccgcc
    13621 agcaccaggc agaagaagcc ggtggcaatc ggcacaaagc gtttgccgcc gaagaagctc
    13681 aggaagtccg gcagtttaat atcggaccaa cggttatagg ctgcgccacc aaccagaccg
    13741 gtaatgatac ccgccagtac acccatgttt atttctgggt tgatggtcac catcgctttg
    13801 gttaacacaa agtaacctac cgcacccgcc agcgccgccg cacctgcgct gtctttcgac
    13861 cagctggatg ccacaccgat ggcgaagatt aatgcgaggt tatcaaaaat cgcaccgccc
    13921 gcctgggcaa taaacgcaac gttaagtaaa tctggctgac cgaatcgcag caacagtgcc
    13981 gccaccggca gcaccgcgat agggagctgt aacgccctac cgagtcgctg gaaaaaacct
    14041 aaaatattca tcttattccc cctacgagaa ccctatttgg ctcgtttaaa gccgtatttt
    14101 tattttgctg caaattgtac tgccgatgtt ctgtaatcag attgttagat catctgctac
    14161 agagtgtgtg aaaatttaat tcgtatcgca aattaaacgc gtgtcttttg tgagttttgt
    14221 caccaaatat cgttatcatc actccctttt actggctaaa ccagaaaact tattttatca
    14281 ttcaaaaaat caggtcggat tgacgcctgt ctgcgcaaat ccaagttacg cttaaagatg
    14341 cctaatccgc caacggctta cattttactt attgaggtga ataatgagac tgatccccct
    14401 gactaccgct gaacaggtcg gcaaatgggc tgctcgccat atcgtcaatc gtatcaatgc
    14461 gttcaaaccg actgccgatc gtccgtttgt tctgggcctg ccaactggcg gcacgccgat
    14521 gaccacctat aaagcgttag tcgaaatgca taaagcaggc caggtcagct ttaagcacgt
    14581 tgtcaccttc aacatggacg aatatgtcgg tctgccgaaa gagcatccgg aaagctacta
    14641 cagctttatg caccgtaatt tcttcgatca cgttgatatt ccagcagaaa acatcaacct
    14701 tctcaacggc aacgccccgg atatcgacgc cgaatgccgc cagtatgaag aaaaaatccg
    14761 ttcttacgga aaaattcatc tgtttatggg cggtgtaggt aacgacggcc atattgcatt
    14821 taacgaaccg gcgtcttctc tggcttctcg tactcgtatc aaaaccctga ctcatgacac
    14881 tcgcgtcgcg aactctcgtt tctttgataa cgatgtcaat caggtgccaa aatatgccct
    14941 gactgtcggt gttggtacat tgctggatgc cgaagaagtg atgattctgg tgctgggtag
    15001 ccagaaagca ctggcactgc aggctgcagt tgaaggttgc gtgaaccaca tgtggaccat
    15061 cagctgtctg caactgcatc caaaagcgat catggtgtgc gatgaacctt ccaccatgga
    15121 gctgaaagtt aagactttaa gatatttcaa tgaattagaa gcagaaaata tcaaaggtct
    15181 gtaattgtta tccctgccct ggctccttgc tcagggcaat attttttaaa atcgggggtc
    15241 agaatgtatg cattaaccca gggccggatc tttaccggcc acgaatttct tgatgaccac
    15301 gcggttgtta tcgctgatgg cctgattaaa agcgtctgtc cggtagcgga actgccgcca
    15361 gagatcgaac aacgttcact gaacggggcc attctctccc ccggttttat cgatgtgcag
    15421 ttaaacggct gcggcggcgt acagtttaac gacaccgctg aagcggtcag cgtggaaacg
    15481 ctggaaatca tgcagaaagc caatgagaaa tcaggctgta ctaactatct gccgacgctt
    15541 atcaccacca gcgatgagct gatgaaacag ggcgtgcgcg ttatgcgcga gtacctggca
    15601 agacatccga atcaggcgtt aggtctgcat ctggaaggtc cgtggctgaa tctggtaaaa
    15661 aaaggcaccc ataatccgaa ttttgtgcgt aagcctgatg ccgcgctggt cgatttcctg
    15721 tgtgaaaacg ccgacgtcat taccaaagtg accctggcac cggaaatggt tcctgcggaa
    15781 gtcatcagca aactggcaaa tgccgggatt gtggtttctg ccggtcactc caacgcgacg
    15841 ttgaaagaag cgaaagccgg tttccgcgcg gggattacct ttgccaccca tctgtacaac
    15901 gcgatgccgt atattaccgg tcgtgaaccg ggcctggcgg gcgcgatcct cgacgaagct
    15961 gacatttatt gcggtattat cgctgatggc ctgcatgttg attacgccaa cattcgtaac
    16021 gctaaacgcc ttaaaggcga caaactgtgt ctggttaccg acgccaccgc gccagcaggt
    16081 gccaacattg aacagttcat ttttgcgggt aaaacaatat actaccgtaa cggactttgt
    16141 gtggatgaga acggtacgtt aagcggttca tccttaacca tgattgaagg cgtgcgtaat
    16201 ctggtcgaac attgcggtat cgcactggat gaagtgctgc gtatggcgac gctctatccg
    16261 gcgcgtgcga ttggcgttga gaaacgtctc ggcacactcg ccgcaggtaa agtagccaac
    16321 ctgaccgcat tcacacctga ttttaaaatc accaagacca tcgttaacgg taacgaggtc
    16381 gtaactcaat aagagaaagt atgacaccag gcggacaagc tcagataggt aatgttgatc
    16441 tcgtaaaaca gcttaacagc gcggcggttt atcgcctgat tgaccagtac gggccaatct
    16501 cgcggattca gattgccgag caaagccagc ttgcccccgc cagcgtaacc aaaattacgc
    16561 gtcagcttat cgaacgcggg ctgatcaaag aagttgatca gcaggcctcc accgggggcc
    16621 gccgcgctat ctccatcgtc accgaaaccc gcaatttcca cgcaatcggc gtacggcttg
    16681 gtcgtcacga cgccaccatc accctgtttg atctcagcag caaagtgctg gcagaagaac
    16741 attacccgct gccggaacgt acccagcaaa cgctggaaca tgccctgttg aatgccattg
    16801 ctcagtttat tgatagctac cagcgcaaac tgcgcgagct gatcgcgatt tcggtgatcc
    16861 tgccagggct tgttgacccg gacagcggca aaattcatta catgccgcat attcaggtag
    16921 aaaactgggg gctggtagaa gctctggaag aacgttttaa agtgacctgt ttcgttggtc
    16981 acgatatccg tagtctggcg ctggcggagc actacttcgg tgcaagtcag gattgcgaag
    17041 actccattct ggtgcgtgtc catcgcggaa ccggggccgg gattatctct aacgggcgca
    17101 tttttattgg ccgcaacggt aacgtcggtg aaattggcca tattcaggtc gaaccgctgg
    17161 gtgaacgctg ccactgcggc aactttggct gcctggaaac tatcgccgcc aacgctgcca
    17221 ttgaacaacg ggtgttgaat ctgttaaagc agggctacca gagccgcgtg ccgctggacg
    17281 actgcaccat caaaactatc tgcaaagccg cgaacaaagg cgatagtctg gcgtcggaag
    17341 taattgagta tatcggtcgt catctgggta aaaccatcgc cattgctatc aacttattta
    17401 atccgcaaaa aattgttatt gccggtgaaa tcaccgaagc cgataaagtg ctgctccctg
    17461 ctattgaaag ctgcattaat acccaggcgc tgaaggcgtt tcgcactaat ctgccggtgg
    17521 tacgttctga gctggatcac cgctcggcaa tcggcgcttt tgcgctggta aaacgcgcca
    17581 tgctcaacgg tattttgctc cagcatttgc tggaaaatta atgtgctttt atagtggcgc
    17641 ttattgttgt caatattctg ggtagtccat gaccattaaa aatgtaattt gcgatatcga
    17701 cggcgtgctg atgcacgata acgtcgccgt accgggtgca gcggaatttt tgcacgggat
    17761 tatggataaa ggcctgccgc tggtgttgct gaccaactat ccttcgcaga ctgggcaaga
    17821 tctggcgaac cgctttgcca ccgcaggtgt cgatgtacct gacagcgtgt tttatacctc
    17881 tgcgatggcg actgccgatt tcctgcgtcg ccaggaaggc aagaaagcgt atgtggtggg
    17941 cgaaggcgca ctgattcatg aactgtacaa agccggtttc actattaccg atgtgaaccc
    18001 tgatttcgtg attgttggcg aaacgagttc ctacaactgg gacatgatgc ataaagcagc
    18061 ctatttcgtc gctaacggtg cacgttttat cgccaccaat ccggacaccc acgggcgcgg
    18121 tttttatccc gcgtgtggcg cgttgtgtgc aggcatcgaa aaaatctccg ggcgtaaacc
    18181 gttctatgtt ggtaaaccga gtccgtggat catccgcgcg gcattaaaca aaatgcaggc
    18241 gcattcggaa gaaacggtga ttgtcggcga taacctgcgt accgatattc tggcgggctt
    18301 ccaggcaggt ctggaaacga ttctggtgct ttctggtgtt tcatcgctcg acgatatcga
    18361 cagtatgccg ttccgcccaa gctggattta cccgtcggtc gctgaaatcg acgttatctg
    18421 aatagcaacc acgtctgcgg acgtggttgg ctctgatgac aaacgccaaa ctgcctgatg
    18481 cgctacgctt atcaggccta cgcagctcct gcaatatatt gaattttcat gcttttgtag
    18541 gccggataag gcgttcacgc cgcatccggc atgaacaaat tgcactttgt cagcaatttg
    18601 gcccccgtaa aatacaaaca ttcaataaaa cccgctgaaa attatcgatt catcaataaa
    18661 tatatcctca caccagacct tttttcacca atcgacaatc accattacgt tttttatttt
    18721 ttcaccgcca aatcgcttgc gcacagtgct gatttgcggc atttttttaa acaagcaaac
    18781 acaacaagca acaaatacca gattaacgga gaaggttatg tgttcaattt ttggcgtatt
    18841 cgatatcaaa acagacgcag ttgagctgcg taagaaagca ctcgagctgt cacgcctgat
    18901 gcgtcatcgt ggcccggact ggtccggtat ttatgccagc gataacgcca ttctcgctca
    18961 cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa
    19021 aacccacgta ctggcggtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata
    19081 tggcgatcgt tatcagttcc agacagggtc tgactgtgaa gtgatcctcg cgctgtatca
    19141 ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga
    19201 cagcgaaaaa gatgcctacc tgattggtcg cgaccatctg gggatcatcc cactgtatat
    19261 gggctatgac gaacacggtc agctgtatgt ggcctcagaa atgaaatcgc tggtgccagt
    19321 ttgccgcacg attaaagagt tcccggcggg gagctatttg tggagccagg acggcgaaat
    19381 ccgttcttat tatcatcgcg actggttcga ctttgatgca gtgaaagata acgtgaccga
    19441 caaaaacgag ctgcgtcagg ctctggaaga ttccgttaaa agccatctga tgtctgatgt
    19501 gccttacggt gtgctgcttt ctggtggtct ggattcctca attatttccg ctatcaccaa
    19561 gaaatacgca gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccgcagtt
    19621 acactccttt gctgtaggtc tgccgggttc accggatctt aaagcagccc aggaagtggc
    19681 aaaccatctg ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc
    19741 catccgcgac gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac
    19801 accgatgtat ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg
    19861 tgaaggttct gatgaagtgt ttggcggtta tctttacttc cataaagcgc ccaacgctaa
    19921 agaactgcat gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg
    19981 cgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa
    20041 attccttgat gtggcgatgc gcattaaccc gcaggataaa atgtgcggta acggcaaaat
    20101 ggaaaaacac atcctgcgtg aatgttttga gtcatacctg cccgcaagcg tggcctggcg
    20161 gcagaaagag cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt
    20221 ggcggcgcag caggtttcta atcagcaact ggaaactgcc cgcttccgct tcccgtacaa
    20281 cacgccaacc tcaaaagaag cgtatctgta ccgggagatc tttgaagaac tgttcccgct
    20341 tccgagcgcc gctgagtgcg tgcctggcgg tccttccgtc gcgtgttctt ccgctaaagc
    20401 gatcgaatgg gatgaagcgt tcaagaaaat ggacgatcca tctggtcgtg cggttggtgt
    20461 tcaccagtcg gcatataagt aagaaatgcg aaacagcccc ggtgaaatcc ggggctgttt
    20521 cagttattga ggtgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac
    20581 atcactgttt gacaactcat cactgtaggc cagtatttca tagattaatt tcccaccact
    20641 attgttgaat tccgaatttc atcctgtacg acaaaagaat ggctgtaggg tggggaaaaa
    20701 acacaaagta aacaataatt gacgaatata gcgccacgct gttcgcaacc taaccaaata
    20761 gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata
    20821 atgcgccccg caacgccgat aaggtatcgc gaaaaaaaag atggctacgt agctcagttg
    20881 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat
    20941 ctttttggcg ggagtggcga aattggtaga cgcaccagat ttaggttctg gcgccgcaag
    21001 gtgtgcgagt tcaagtctcg cctcccgcac cattcaccag aaagtgttgt acggatgggg
    21061 tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt
    21121 accccagcca tcttcttcga gtaagcggtt caccgcccgg ttattggggt atcgccaagc
    21181 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
    21241 cgaagaaaca atctggctac gtagctcagt tggttagagc acatcactca taatgatggg
    21301 gtcacaggtt cgaatcccgt cgtagccacc aaattctgaa tgtgtcgaat acgttcggca
    21361 aattcaaaaa ccaatttgtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg
    21421 gcattccgag gttcgaaccc tcgtacccca gccaatttat tcaagacgct tacatcgtaa
    21481 gtgcacccag ttggggtatc gccaagcggt aaggcaccgg attctgattc cggcattccg
    21541 aggttcgaat cctcgtaccc cagccacatt aaaaaagctc gcttcggcga gctttttgct
    21601 tttctgcgtt cattcaatgt cgaatgcgat gttgacacgt cttatccttc aatgtcggat
    21661 gcgacgctgc cgcgtcttat ccgacctacg gttggcacgc atccggcaat gttgtaaggc
    21721 tacaacccta acgcatattt cagcgcctga cgtttcaaca cgccagcacg ctctgccgcc
    21781 attaacccga gattacgcac aaaacgcagc ggcggcagat tattactgaa tccggcataa
    21841 aacagatcca taccactttg cataatgaag ttatccgcca tgcgccgcat ctggtaacgc
    21901 ttgaggacag gataactggc ccacgcttca ccgtaactgc gggcattcac cagaacatca
    21961 atcagggcat cgacatcacg ataaccaaga ttcaccccct gccccgccag cggatggatg
    22021 gtatgcgcgg catcgcccac cagcgcaagc cccggctgca catactgcaa cgcatgtcgg
    22081 cgcgtcagcg gaaacgcacc agcggcaagc ggtgtaacgt aacccagacg cgacgggaaa
    22141 tgcttcgcga tttccgcctg gagttgcgcc atattcatat tctgcaactg gcgaatacgc
    22201 gccggagagt cataccacac cagcgatgcc cagttatcaa acaacggcag aaacgcacgc
    22261 ggtccgtccg gggtaaattg ctgccaggtg ctgtcgccgg gatcgttctc acactgtacg
    22321 ctaatcaaca tgcacgactg cgcatactgc caggcatgaa cgccaattcc cgccatctgc
    22381 cgcacctgag aatttgcgcc gtcggcacca atcaccagtt tcgcgcggat cgtttcaccg
    22441 cccttcagtt ccagctcctg aagatcatta tctcgatgta atgcaatcag cgagcctggc
    22501 acacgtaacg ttactttcgg atgcgcttcc agcgcctgcc acagcgcctg ttgcaggacg
    22561 gtgttttcca ccatatagcc aagcaacggt agcttaagtt cagcggcgtc aaacaccaca
    22621 tgcgccgttt cccactccca cgtttccagt ctgcggtaag gatggcaacg catagcctgt
    22681 actgcatccc agacccctaa ccctttaagc aatgataccg atgcggcgct gatcgccgag
    22741 atccgcacgt ccggttggct atcagcgaca aacggtgcag gttctgcgtg ctcgatcacc
    22801 gttaccgaaa atccgtgctg tgccagcccc agcgccagcg cgccgccgac cattcctccg
    22861 ccgacaatgg caatttccgt tggttgattt gtcatggtca atcatcctgt tagcaatatg
    22921 ctttaagttt acaggatttt tacccccaag ggctgataac gctcatactg gccacaacgg
    22981 cagcaaagca ttacactatg cgccctgcat tcctggctac tatttcgcaa gagcaagtcg
    23041 atgaccaaaa aactccatat taaaacctgg ggctgtcaga tgaacgagta cgattcatcg
    23101 aagatggccg atctgctgga tgccacccac ggctatcaac tgaccgacgt ggcggaagaa
    23161 gcggatgtgc tgctgctgaa cacctgctca atccgcgaga aggctcagga aaaagtcttc
    23221 catcagttgg gtcgctggaa actgttaaaa gagaagaatc cagacctgat tatcggcgtc
    23281 ggtggctgcg tggcatcgca agaaggcgag cacattcgcc agcgcgccca ctatgtcgat
    23341 attatttttg ggccgcaaac gctgcaccgt ctgccggaga tgatcaactc cgtgcgcggc
    23401 gaccgcagcc cggttgtaga tatcagcttc ccggaaatcg agaagtttga ccgtctaccg
    23461 gaaccgcgcg ccgaagggcc gaccgcgttt gtctccatca tggaaggctg caataaatat
    23521 tgcacctact gcgtggtgcc ttacacccgt ggtgaagagg taagccgtcc gtctgacgat
    23581 attctgtttg agattgccca gcttgcggct cagggcgtgc gtgaagtcaa cctgctcggt
    23641 cagaacgtga acgcctggcg tggtgaaaac tacgacggct ccaccggatc gtttgccgat
    23701 ctgctgcgtc tggttgctgc gatcgacggg atcgatcgta ttcgctttac caccagccat
    23761 ccgatcgaat tcaccgacga tatcatcgaa gtgtatcgcg acacgccgga gctggtgagc
    23821 ttcctgcatc tgccggtaca gagcggttcc gatcgcattc tgaacctgat gggccgtact
    23881 catacggcac tggagtacaa agcgatcatc cgtaaactgc gtgcggcgcg tccagatatt
    23941 cagatcagtt ctgatttcat cgttggcttc cctagcgaaa ccaccgaaga cttcgagaaa
    24001 acgatgaagc tgattgccga cgtcaatttc gacatgagct acagctttat cttctctgca
    24061 cgtccgggta caccagccgc cgatatggtt gatgatgttc cggaagaaga gaagaagcag
    24121 cgtctgtata ttctgcaaga acgtatcaat cagcaagcga tggcatggag ccgccggatg
    24181 ctcggcacca cccagcgtat tctggtagaa gggacttcgc gtaagagcat catggagctt
    24241 tccgggcgta ccgaaaataa ccgcgtggtc aacttcgagg gcacaccgga tatgatcggt
    24301 aaattcgtcg atgtagaaat taccgacgtc tacccgaact ctctgcgcgg taaagtggtg
    24361 cgtactgaag atgaaatggg cctgcgcgtg gcagaaacac cggaatcggt gattgcccgt
    24421 acccgcaaag aaaacgacct tggcgtgggt tattatcagc cgtaattctc aggcccgccg
    24481 ttccggtggg ccttgttttt tctctcttta tccccatctt tatcgcaatg cttgcgcctt
    24541 tttcctgtgg cgtgaataat ttccttatag gccggtgaat gttcagcttc cggcggcata
    24601 cagataagag gaacggtttg aacatagaca ctcgcgaaat caccctggag ccagcagaca
    24661 acgcgcgtct gttgagcctg tgcggcccgt ttgatgacaa catcaagcag ctcgaacgcc
    24721 gtctcggcat cgagatcaat cgccgcgaca accactttaa actgaccggc cgtccgattt
    24781 gcgtcaccgc tgcggcagac attctgcgta gcctgtatgt cgatacagcc ccgatgcgcg
    24841 gtcagattca ggatatcgaa ccggaacaga tccaccttac gattaaagaa gcgcgagtac
    24901 tggagcaaag tgcggagagc gtgccggagt acggcaaagc ggtcaatatc aaaaccaaac
    24961 gcggcgtaat taagccgcgc acgccaaacc aggcgcagta catcgccaat attctcgacc
    25021 atgacattac cttcggcgtt ggcccggcgg gtacgggtaa aacctacctg gcagtggctg
    25081 cggcagttga tgccctggag cgtcaggaga ttcgccgtat tctgctgact cgtccggcgg
    25141 tcgaagccgg tgagaaactg ggcttcctgc ctggcgattt aagccagaaa gtagacccgt
    25201 atctgcgccc actgtacgac gcgctgtttg aaatgctggg ctttgagaaa gttgagaaac
    25261 tgattgagcg caacgttatt gaagtcgcgc cgctggccta tatgcgtggt cgtacgctga
    25321 acgacgcgtt tatcattctc gatgagagcc agaacaccac catcgaacag atgaagatgt
    25381 tcctgacccg tatcggtttt aactcaaaag cggttatcac cggcgacgtc acacagatcg
    25441 acctgccgcg taatactaaa tcaggcttac gtcacgccat cgaagtactg gccgatgtcg
    25501 aagagatcag ctttaacttc ttccacagcg aagacgtggt tcgtcaccct gtggtggcgc
    25561 gtatcgttaa cgcctatgaa gcctgggaag aagccgaaca aaaacgaaaa gcggcgctgg
    25621 cggcagaacg caagcgcgaa gaacaggaac aaaaatgagt caggtgatcc tcgatttaca
    25681 actggcatgt gaagataatt ccgggttacc ggaagagagc cagtttcaaa catggctgaa
    25741 tgcggtgatc ccgcagtttc aggaagaatc ggaagtgact attcgcgtgg tcgataccgc
    25801 cgaaagccac agtctgaatc tgacctatcg cggtaaggat aagccgacca acgtgctctc
    25861 cttcccgttt gaagtgccgc ctggcatgga aatgtcgcta ctgggcgatc tggttatctg
    25921 ccgtcaggtg gttgagaagg aagctcagga gcaaggcaaa ccactggagg cgcactgggc
    25981 gcatatggtg gtgcacggca gtctgcattt gttaggttac gatcacatcg aagatgacga
    26041 agcagaagaa atggaagccc tcgaaacaga gattatgctt gctctgggct atgaggatcc
    26101 gtacattgcc gagaaagaat aatttgccag gcatttgact ggcagacgtc cccgccgttg
    26161 gtgattaacg acggcgtttt gattaactaa catgagaacc catacgacgc catgagcgac
    26221 gacaattcac acagtagtga cactataagc aacaagaagg gatttttctc cctgttactc
    26281 agccaacttt tccacggtga accgaaaaac cgtgacgaac tgctggcgct gatccgtgat
    26341 tccgggcaga acgaccttat cgacgaagat acgcgcgata tgctcgaagg ggtgatggac
    26401 atcgcagacc aacgcgttcg cgacatcatg atcccccgct cccagatgat taccctgaaa
    26461 cgcaaccaga cgctggacga atgccttgat gtcatcatcg agtccgccca ctcacgtttc
    26521 ccggtgatta gcgaagacaa agatcacatt gaagggattc tgatggcgaa agacttgctg
    26581 ccgtttatgc gcagcgatgc tgaagccttc agcatggaca aagtgttacg tcaggcggtt
    26641 gtcgttcctg aaagtaagcg cgtagaccgg atgctgaaag agtttcgctc tcagcgttac
    26701 cacatggcga tcgtcattga cgaattcggt ggggtttccg gtctggtgac cattgaagac
    26761 atcctggaac tgattgttgg tgagattgaa gacgaatatg acgaagaaga tgatatcgac
    26821 ttccgtcagc tgagtcgtca tacctggacc gtgcgcgcac tggcttccat tgaagacttc
    26881 aacgaagcgt tcggcaccca ctttagcgat gaagaagtcg acactatcgg tggtctggtg
    26941 atgcaggcgt ttgggcatct tccggcgcgt ggcgaaacta tcgacatcga cggttaccag
    27001 ttcaaagtgg cgatggccga cagtcgacgt attattcagg ttcatgtcaa aatcccggat
    27061 gactcacccc agccgaagct ggatgaataa aaccgaaact ggatagataa ctacatggct
    27121 tttgcctcat taattgaacg ccagcgcatt cgcctgctgc tggcgttatt attcggtgcc
    27181 tgcggaacgc tggccttctc tccttacgac gtctggcctg cggcgattat ttcgttgatg
    27241 gggcttcagg cgttgacctt taaccgccgt ccactccagt ctgccgctat tggcttttgc
    27301 tggggatttg gcctctttgg cagcggtatt aactgggtct atgtcagcat cgcgaccttt
    27361 ggcggaatgc ctggcccggt taacatcttc ctggtggtgc tgctggcggc gtatttgtcg
    27421 ctgtataccg gactgtttgc tggcgtgctg tcgcgtctgt ggccgaaaac cacctggctg
    27481 cgcgtggcga ttgccgcccc tgctctctgg caagtgaccg agtttctgcg cggttgggta
    27541 ctgacaggct tcccgtggtt acagttcggc tatagccaga ttgatggccc gttaaaaggg
    27601 ctggcaccga taatgggcgt ggaagccatt aacttcctgc tgatgatggt tagtggcctg
    27661 ctggcactgg cgttggtcaa acgcaactgg cgtccgctgg tggtggccgt cgtgctgttt
    27721 gccctaccct tcccgctgcg ttacatccaa tggtttaccc cgcaaccgga gaaaaccatt
    27781 caggtttcga tggttcaggg cgatattccg caatcactga aatgggatgg agatcagctt
    27841 cttaatacgc tgaagattta ctacaacgca acggctccgc tgatgggcaa atcatcgttg
    27901 attatctggc cggagtcggc gataaccgat ctggaaatta atcagcaacc gttcctcaaa
    27961 gcactggacg gtgagttgcg cgataaaggt agctcgctgg taaccgggat tgtcgacgcg
    28021 cgtctcaata agcagaaccg ctacgatacc tacaacacca tcatcacact gggtaaaggt
    28081 gcaccgtaca gctacgaatc agccgatcgc tataacaaaa accatctggt gccgtttggc
    28141 gagtttgtcc cgctggagtc gattctgcgt ccgttagcac cgttctttga tctgccgatg
    28201 tcgtcgttca gccgtgggcc atatatccag ccgccgctgt cagtaaatgg tattgagctt
    28261 actgcggcta tttgctacga gatcattctc ggcgagcaag tgcgcgataa cttccgcccg
    28321 gataccgact atctgctgac tatctccaac gatgcgtggt ttggtaaatc tattggtcca
    28381 tggcaacact tccagatggc gcgaatgcgt gcgctggagc tggcgcgccc actgttgcgc
    28441 agcaccaaca acggcattac ggcggtgatt ggcccgcagg gtgagattca ggcgatgatc
    28501 ccgcagttca cccacgaggt gttaaccact aatgtgacac cgaccaccgg actcacaccg
    28561 tatgcacgta ccggcaactg gccactgtg
//
LOCUS       DAJTYD010000034        28512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTYD010000034 DAJTYD010000000
VERSION     DAJTYD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.034"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            130..2979
                     /gene="rcsC"
                     /locus_tag="OO585_001363"
     CDS             130..2979
                     /gene="rcsC"
                     /locus_tag="OO585_001363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6629000.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3179..3829)
                     /gene="rcsB"
                     /locus_tag="OO585_001364"
     CDS             complement(3179..3829)
                     /gene="rcsB"
                     /locus_tag="OO585_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6629001.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(3846..6518)
                     /gene="rcsD"
                     /locus_tag="OO585_001365"
     CDS             complement(3846..6518)
                     /gene="rcsD"
                     /locus_tag="OO585_001365"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6629002.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7257..8387
                     /gene="ompC"
                     /locus_tag="OO585_001366"
     CDS             7257..8387
                     /gene="ompC"
                     /locus_tag="OO585_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6629003.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8499..9554
                     /gene="apbE"
                     /locus_tag="OO585_001367"
     CDS             8499..9554
                     /gene="apbE"
                     /locus_tag="OO585_001367"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6629004.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9628..10680
                     /gene="ada"
                     /locus_tag="OO585_001368"
     CDS             9628..10680
                     /gene="ada"
                     /locus_tag="OO585_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6629005.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCPVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10680..11330
                     /gene="alkB"
                     /locus_tag="OO585_001369"
     CDS             10680..11330
                     /gene="alkB"
                     /locus_tag="OO585_001369"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6629006.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11406..13049
                     /gene="yojI"
                     /locus_tag="OO585_001370"
     CDS             11406..13049
                     /gene="yojI"
                     /locus_tag="OO585_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6629007.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13267..14913
                     /gene="mqo"
                     /locus_tag="OO585_001371"
     CDS             13267..14913
                     /gene="mqo"
                     /locus_tag="OO585_001371"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6629008.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15063..15551)
                     /gene="eco"
                     /locus_tag="OO585_001372"
     CDS             complement(15063..15551)
                     /gene="eco"
                     /locus_tag="OO585_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6629009.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15686..15850)
                     /locus_tag="OO585_001373"
     CDS             complement(15686..15850)
                     /locus_tag="OO585_001373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629010.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            15959..16453
                     /gene="napF"
                     /locus_tag="OO585_001374"
     CDS             15959..16453
                     /gene="napF"
                     /locus_tag="OO585_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6629011.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16443..16706
                     /gene="napD"
                     /locus_tag="OO585_001375"
     CDS             16443..16706
                     /gene="napD"
                     /locus_tag="OO585_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6629012.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            16703..19189
                     /gene="napA"
                     /locus_tag="OO585_001376"
     CDS             16703..19189
                     /gene="napA"
                     /locus_tag="OO585_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6629013.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19196..19891
                     /gene="napG"
                     /locus_tag="OO585_001377"
     CDS             19196..19891
                     /gene="napG"
                     /locus_tag="OO585_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6629014.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            19878..20741
                     /gene="napH"
                     /locus_tag="OO585_001378"
     CDS             19878..20741
                     /gene="napH"
                     /locus_tag="OO585_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6629015.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            20738..21187
                     /gene="napB"
                     /locus_tag="OO585_001379"
     CDS             20738..21187
                     /gene="napB"
                     /locus_tag="OO585_001379"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6629016.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21197..21799
                     /gene="napC"
                     /locus_tag="OO585_001380"
     CDS             21197..21799
                     /gene="napC"
                     /locus_tag="OO585_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6629017.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            21818..22435
                     /gene="ccmA"
                     /locus_tag="OO585_001381"
     CDS             21818..22435
                     /gene="ccmA"
                     /locus_tag="OO585_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6629018.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22432..23094
                     /gene="ccmB"
                     /locus_tag="OO585_001382"
     CDS             22432..23094
                     /gene="ccmB"
                     /locus_tag="OO585_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6629019.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23136..23873
                     /gene="ccmC"
                     /locus_tag="OO585_001383"
     CDS             23136..23873
                     /gene="ccmC"
                     /locus_tag="OO585_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6629020.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            23870..24079
                     /gene="ccmD"
                     /locus_tag="OO585_001384"
     CDS             23870..24079
                     /gene="ccmD"
                     /locus_tag="OO585_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6629021.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24076..24555
                     /gene="ccmE"
                     /locus_tag="OO585_001385"
     CDS             24076..24555
                     /gene="ccmE"
                     /locus_tag="OO585_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6629022.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24552..26501
                     /gene="ccmF"
                     /locus_tag="OO585_001386"
     CDS             24552..26501
                     /gene="ccmF"
                     /locus_tag="OO585_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6629023.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     MLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIAIASLLSGRLDSTYARFTRPWTLAAW
                     IFLTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKA
                     WTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAA
                     RGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPF
                     FNTMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKV
                     VAMTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQN
                     YSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEK
                     RYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMAL
                     GGLLCLFDPRYRKRVSPQKTAPEAV"
     gene            26498..27055
                     /gene="dsbE"
                     /locus_tag="OO585_001387"
     CDS             26498..27055
                     /gene="dsbE"
                     /locus_tag="OO585_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6629024.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27052..28104
                     /gene="ccmH"
                     /locus_tag="OO585_001388"
     CDS             27052..28104
                     /gene="ccmH"
                     /locus_tag="OO585_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6629025.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28139..>28512)
                     /locus_tag="OO585_001389"
     CDS             complement(28139..>28512)
                     /locus_tag="OO585_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6629026.1"
                     /translation="SDVFALIDAGADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQ
                     YLREREMFGAEEDPFSVLTERELDVLHELAQGLSNKQIASVLNISEQTVKVHIRNLLR
                     KLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6491 a         7035 c         7934 g         7052 t
ORIGIN      
        1 tttcctggtt aaggcaatgg tttctaagcg atacgcatta ttgcaataat gacagaacat
       61 tgcggtaaca cgcttttacc gctaccttaa ccacactcca tcggtcacct gaggcggagc
      121 ttcgcccctt tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg
      181 ttcagagcat tggcgttagt gctctggctg ttgattgctt tttcatccgt tttttacatc
      241 gttaatgcgt tacatcagcg agaatcggaa attcgtcagg aatttaatct gagttccgat
      301 caggctcagc gctttattca acgcacctct gatgtgatga aagagctgaa gtacatcgcc
      361 gaaaatcgct tatcggcaga aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg
      421 gatgtgcctg cgtttgaacc gctgtttgcc gactccgatt gttccgcaat gagtaacacc
      481 tggcgaggtt ctctggagtc attggcgtgg tttatgcgct actggcgcga taatttttct
      541 gcggcttacg atctaaaccg ggtattttta atcggcagcg ataacctctg catggccaat
      601 ttcggtctgc gtgatatgcc agtggaacgc gataccgcgt tgaaagcttt gcatgaacgc
      661 atcaataaat atcgaaatgc accacaagat gatagcggca gtaacctcta ctggatcagc
      721 gaaggtccgc gccctggcgt cgggtatttt tacgcgttga cgccagttta tctggcgaac
      781 cggttgcagg cgcttttggg gggcgagcag accatccgga tggagaactt tttcttaccg
      841 ggtacgttgc cgatgggggt taccattctt gatgaaaatg gtcataccct gatttcgctt
      901 accggaccag aaagtaaaat taagggcgag cctcgctgga tgcaggaacg ctcctggttt
      961 ggctatacgg aagggttccg ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta
     1021 agcatcgtgt attcggtgcc ggttgataag gtgctggaac gcattcgcat gttgatcctt
     1081 aacgcaattt tgctgaatgt gcttgccgga gctgcattgt ttactctcgc acggatgtac
     1141 gagcgacgta ttttcattcc ggcggaaagc gacgccctgc gactggaaga acatgagcag
     1201 ttcaatcgca agattgtcgc ctccgcgcca gtgggtatct gcattttgcg taccgctgat
     1261 ggcgtcaata ttttaagtaa cgaactggcg catacctatc tcaatatgct tacgcatgag
     1321 gaccgccaac gactgacgca aattatctgt gggcagcagg tcaattttgt tgatgtcctg
     1381 accagcaaca ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac
     1441 gtggccattt gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag
     1501 gagatggcac aagcagcgga acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc
     1561 agtcatgagc tgcgaacgcc gctgtatggc attatcggta acctggatct gttgcaaacc
     1621 aaagagttac cgaaaggcgt cgatcggctg gtgacggcaa tgaacaactc ttccagcctg
     1681 ttgttgaaaa ttatcagcga tattctcgat ttctcgaaga ttgaatcgga acagttgaag
     1741 atcgaaccgc gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc caactattta
     1801 ccgctggtgg tacgcaagca gttaggcttg tactgcttta ttgaaccgga tgtgccagtg
     1861 gccttaaatg gcgacccgat gcgtttacag caggtcatct ccaacctgtt gagtaacgcc
     1921 ataaaattca ccgataccgg ctgtatagtt ttgcatgtcc gcgctgatgg cgattatctc
     1981 tctatccgtg ttcgcgatac cggcgtgggg attccggcga aagaagtggt gcgcttgttt
     2041 gatcccttct tccaggtcgg aacgggcgta cagcgaaact tccaggggac cggtctgggt
     2101 ctggcgattt gtgaaaaact gatcagcatg atggacggcg atatctcggt agattcagaa
     2161 ccgggaatgg gcagccagtt taccgtgcgt attccgttgt acggcgctca gtacccgcag
     2221 aaaaaaggcg tggaagggtt gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg
     2281 ctctgtcagt tcctagaaac tagtttgcag cgcagcggca tcgtcgttac aacatacgaa
     2341 gggcaggaac cgactcccga agatgtgtta attactgacg aggtagtgag taaaaaatgg
     2401 cagggtagag cggtagtgac cttctgtcgt cgccatattg gtattccgct ggagaaagcg
     2461 ccaggggagt gggtacacag tgtggctgcc ccgcatgagc taccggcatt gttggcgcgt
     2521 atttatttga tcgagatgga gagcgacgat cctgctaacg ctctgccgtc gacggacaaa
     2581 gcggtcagcg ataatgacga tatgatgatt ctggtcgtgg atgatcatcc gattaaccga
     2641 cgtctgctgg cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc gaatgatggt
     2701 gtcgatgcgc ttaatgtact tagcaagaat catattgata tcgtgcttag cgacgtcaac
     2761 atgccaaata tggatggtta ccgcttgacg caacgcattc gtcagttggg actgacgttg
     2821 ccggtaatcg gagtaactgc taatgcgttg gctgaagaga agcagcggtg tctggagtcc
     2881 ggtatggaca gctgcctgtc gaagccggta acgctggatg tgataaaaca gacgctgacg
     2941 gtatatgccg agagggtcag gaaatcgcgg gaatcgtagg acggataagg tgtttatgtc
     3001 gcatctggca ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc tggcctactt
     3061 tgatgtaatc tgttgaaata atgggaatcg taggacgcat aaggcgttta cgccgcatcc
     3121 ggcaatcggt gcaaatgcta gataagacac taacgcgtct tatctggcct acaggtgatt
     3181 agtctttatc tgccggactt aacgtcactg aagagagata attcagcagg gcgatatcgt
     3241 tctcgacacc cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc
     3301 ggttcagctt tttagcgatc tcggtcacca agaagccttc cgcaaacagg cgcagaactt
     3361 cactctcttt tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca
     3421 ggcgagaaac gctttccggg gtaaatttct tccctttctg cagcgcggcg agagctttcg
     3481 gcagatcggt cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg
     3541 cactaagaat cgccgggttg ttgttcatag tcagaacaat gatcgacagg cttgggaaat
     3601 ggcgcttgat gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga
     3661 gatcggtaat caacacatgc gcatccagtt tcggcaggtt gttgatcagt gctgtagagt
     3721 cttcaaattc gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga
     3781 acaagactat cggatggtca tcggcaataa ttacgttcat attgttcatg taataggcta
     3841 ccttgctaca gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct
     3901 ggaacatcct tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc
     3961 agatttagca tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag
     4021 tcactggttg ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag
     4081 agcgcataat agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct
     4141 gtcacctctt cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg
     4201 ttgctcatat tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca
     4261 tcatcgctta aaagcaagcc agaggcagta agattagacg gattatccgt taaaaagata
     4321 tcataatctt gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct
     4381 aactggcgag tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca
     4441 tcatccagta aacgctcttc ttcctcttca acttccgggt cagcggcgag cattttgata
     4501 tgcacagagt agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt
     4561 ttacgcgcca gttgatcgct cagccagaat gccagcgggt ccgccttgcc atagcgatcg
     4621 ttttggggct ggttgataaa cgggaagtgc aaattatcca tttcatgaat acttacgcct
     4681 tctcccgtgt ccagaatgcg gaatgtcagg cggtcttcag aggactcatc ctgatcaacc
     4741 tcaagggtga tttttcccaa ttgcgttgag gtcacggcat attgcatcag tagcagcaaa
     4801 atacgtcgta aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag atgattgtta
     4861 atcagcagtt gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca
     4921 attaaatcct gcacggagaa cagcaccgtc tcacttttcc agctatcgtc cgcaagcatg
     4981 ttcgctaact gtatttcatc gaccagccgc accagcacat ctgcctgatt cgccagttgt
     5041 ttgctttccg gggcgttgag tttagccgcg ctctccgcca gggactgtgc gggttctttc
     5101 agcgcatcgc caatgttttt cataaaggtc atccgcccct gctggttttt ctcatacaga
     5161 cgctgcgcct gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg
     5221 aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc ggatctcata cagctcgtta
     5281 ttgatcgtcg cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga
     5341 ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc
     5401 tgatcgtgaa ccagcaggcc gagcggcagc agtgagacta tctcttcatt aattgcccgt
     5461 aaaatgcgca attcgttatt gaccgccgtg ctgggtagac tttctgtact gcggctggag
     5521 aaatggcgga atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc
     5581 agtggcagca gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag
     5641 accaggcgca tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag
     5701 tggatactga cgctatccgt cccttctttc tcattatcat tgtctcccgt tgccgtcgcg
     5761 tctggctcaa ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggt
     5821 agatcaaaag ctacgaccgt tgccagatgt cccggctggt tgaacgtggt acgcaaggta
     5881 aagtaatgac cgttctgcca ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg
     5941 agggcgttgg cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgatggtc
     6001 gattctttaa atccggaggt gagatctttg aggggcaggg ttgagatcag caccagactg
     6061 ttatcctgac cattcaggta atacatcgac cacggtacat tttctgcgcc ccacaatgtg
     6121 tccagatagg tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag
     6181 attaacgctt ccgttttgcg gcgcggtttc tccagatagt agacatcctg tttcaggcgc
     6241 gtctcttgta aaccttcgcc ggaggagggg gaggttgtcg cggcaatgtt gtcgtagatc
     6301 tgccaggtca cgtaacgcca ggtattgacg cgcttttgga tagcgtgggt aatgtcgaca
     6361 atctggtaac ttttatcttt cagccatgcg ttaacggcgc tttgtaccat tacacccatc
     6421 gtcaccagta acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt
     6481 gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa
     6541 cttcgcgaag tgatagggca gtataaaggg tacagtgtga aattccagac tcttacctga
     6601 ttgatgattc tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac
     6661 aagcgccatt ttgctgtaca aataacctac aaaaagccca acgaaattaa ttgcaaataa
     6721 acgatgattg ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagcgtta
     6781 tagtttttct gtggtagcac agaataatga aaagtgtgta aagaagggta aaaaaaaccg
     6841 gatgcgaggc atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat
     6901 aaataaagtt aatgatgata gcgggagtta ttctagttgc gagtgaaggt tttgttttga
     6961 cattcagtgc tgtcaaatac ttaagaataa gttattgatt ttaaccttga attattattg
     7021 cttgatgtta ggtgcttatt tcgccattcc acaataatct taaaaagttc ccttgtattt
     7081 acattttgaa acatctatag cgataaatga aacatcttaa aagttttagt atcatattcg
     7141 tgttggatta ttctgcattt ttggggagaa tggacttgcc gactgattaa tgagggttaa
     7201 tcagtatgca gtggcataaa aaagcaaata aaggcatata acagagggtt aataacatga
     7261 aagttaaagt actgtccctc ctggtcccag ctctgctggt agcaggcgca gcaaacgctg
     7321 ctgaagttta caacaaagac ggcaacaaat tagatctgta cggtaaagta gacggcttgc
     7381 actatttctc tgacaacaag tcagaagacg gcgaccagac ctatgtacgt cttggtttca
     7441 aaggcgaaac tcaggttact gaccagctga ccggttacgg ccagtgggaa tatcagatcc
     7501 agggcaatac ctctgaagac aacaaagaaa actcctggac ccgtgtggca ttcgcaggtc
     7561 tgaaattcca ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg
     7621 taacttcctg gaccgacgta ctgccagaat tcggtggcga cacctacggt tctgacaact
     7681 tcatgcagca gcgtggtaac ggcttcgcga cctaccgtaa caccgacttc ttcggtctgg
     7741 ttgacggtct gaactttgct gttcagtacc agggcaaaaa cggtagcgta agcggcgaag
     7801 gcatgaccaa caacggtcgt ggcgctctgc gtcagaacgg cgacggtgtc ggcggttcta
     7861 tcacttatga ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg
     7921 atgatcaaaa tggtagctac atcagcaatg gtgtagttcg taactacatc ggtactggcg
     7981 accgtgctga aacctacact ggtggtctga aatacgacgc taacaacatc tacctggctg
     8041 ctcagtacac ccagacctac aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag
     8101 cacagaactt cgaagctgtt gctcagtacc agttcgactt cggtctgcgt ccgtctgtag
     8161 catacctgca gtctaaaggt aaaaacctgg gtgtcatcaa tagtcgtaac tacgacgacg
     8221 aagatatcct gaaatatgtt gatgttggcg cgacctacta cttcaacaaa aacatgtcca
     8281 cctatgttga ctacaaaatc aacctgctgg acgacaacca gttcactcgt gacgctggca
     8341 tcaacactga taacatcgta gctctgggtc tggtttacca gttctaatct cgattgatat
     8401 cgaacaaagg gcctgcgggc ccttttttca ttgttttcag cgtacaaact cagttttttg
     8461 gtgtactctt gcgaccgttc gcatgaggat aatcacgtat ggaaataagc tttacccgcg
     8521 tggcactgct ggctgccgcg ctcttctttg ttggttgcga tcaaaaacca caacccgcca
     8581 aaacccacgc tactgaagtt accgttcttg aaggcaaaac catgggtacc ttctggcgtg
     8641 ccagcatccc gggcattgat gccaaacgca gtgccgaact taaagaaaag attcagaccc
     8701 agctggacgc cgacgatcag ctgctttcga cctataaaaa agattccgcg ctgatgcgct
     8761 ttaacgactc gcaaagtttg tcgccgtggc cagtaagtga agcgatggcc gaaatcgtca
     8821 ctacctcgct gcgcattggc gcgaagaccg atggtgcgat ggatattacc gtcgggccgc
     8881 tggtaaatct gtggggcttt ggcccggaac aacagccggt tcaaattccg agccaggaac
     8941 agatcgacga gatgaaagcc aaaactggtt tacagcacct tacggtcatt aatcagtcgc
     9001 atcagcaata tctgcaaaaa gacctgccgg atttatatgt cgatctctct accgtcggcg
     9061 aaggttatgc ggcggatcac ttggcacgct tgatggagca ggaagggatt tcccgctatc
     9121 tggtgtcggt gggcggcgcg ctgaacagcc gtggtatcaa cggtgaaggc ctgccgtggc
     9181 gggtagcgat tcaaaaacca accgataaag aaaacgcagt tcaggctgtg gtggatatca
     9241 acggtcatgg gattagcacc tctggcagct accgtaacta ttacgaactg gacggcaaac
     9301 gtctttccca tgttatcgat ccgcaaaccg ggcgtcccat cgaacacaat ctggtatccg
     9361 tgacggtgat tgctccgacg gcgctggaag ccgatgcctg ggatactggc ttgatggtac
     9421 tcgggccgga gaaagccaaa gaggttgttc gccgggaagg gctggcggtt tatatgatca
     9481 ccaaagaagg cgatagcttt aaaacctgga tgtcaccaca gtttaaaagc ttccttgtca
     9541 gcgaaaaaaa ttaaagcgca agattgttgg tttttgcgtg atggtgaccg ggcagactaa
     9601 aggctatcct taaccaggga gctgattatg aaaaacgcca catgcttaac tgacgatcaa
     9661 cgctggcaat ctgtcttagc ccgcgacccg aatgccgacg gcgaattcgt tttcgccgtg
     9721 cgcaccacag gcatcttttg ccgtccgtct tgccgcgcca gacatgcctt gcgggaaaat
     9781 gtctccttct acgcaaatgc cagcgaggca ctcgccgccg gatttcgccc ctgcaaacgc
     9841 tgccaacctg ataaagccaa tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt
     9901 cgactgctgg aacaggaaac gcctgtaacg ttggaagcct tagccgacca ggtggcgatg
     9961 agtccatttc atctacatcg gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg
    10021 caacaggcct ggcgcgctcg ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg
    10081 acgtctattc ttaacgccgg attccccgac agcagcagtt actaccgcaa agccgatgaa
    10141 acgctgggca tgacggctaa acaattccgt cacggtggcg aaaatctggc ggtgcgttac
    10201 gcgctggctg attgtgcgct gggtcgttgc ccggtggcag aaagcgagcg ggggatttgc
    10261 gcgatattgc tgggcgatga tgacgcgaca ttaatcagcg agttgcagca gatgtttccc
    10321 gctgccgaca acgcgcctgc cgatctgacg tttcagcaac atgtgcgtga agtgatcgcc
    10381 agcctcaatc aacgcgatac gccgctgacg ttaccgctgg atattcgcgg cactgctttt
    10441 cagcaacaag tctggcaggc actgcgcacg ataccttgcg gtgaaaccgt actggctaac
    10501 gccatcggca aaccgaaagc ggtacgggcc gttgccagcg cctgtgccgc caacaagctg
    10561 gctatcgtaa taccctgtca tcgagtggtc ggtggtgatg gcacactttc cggttaccgc
    10621 tggggcgtgt ctcgtaaagc gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa
    10681 tgttggatct gtttgccgat gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa
    10741 ttttacggcg ttttgctttt aacgctgcgg agcaactgat ccgcgatatt aatgacgttg
    10801 ccagccagtc gccgtttcgc cagatggtca cccccggggg atataccatg tcggtggcga
    10861 tgaccaactg tgggcatctg ggctggacga tccatcggca aggttatctc tattcgccca
    10921 ttgatccgca aacaaataaa ccgtggcccg ccatgccaca aagctttcat gatttatgtc
    10981 aacgtgcggc tacggcggcg ggctatccag atttccagcc agatgcttgt cttatcaacc
    11041 gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg
    11101 cgccaattgt ttctgtttct ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac
    11161 gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga tgtggtggta tggggcggtg
    11221 aatcgcggct gttttatcac ggtattcaac cgttgaaagc ggggttttat ccactcacca
    11281 ccgactgccg ctacaacctg acattccgtc aggcaggtaa aaaagaataa aaataagaat
    11341 tattattgct gtgcgcgcga agatgtttaa actgcgggct gtaattcatt gtccgggttt
    11401 tctgcatgga acttcttgta cttgtctggc ggcagtatcg ctggccattt atcagtgtga
    11461 tggcgctaag cctcgccagt gcggcattag gcattggctt aattgctttt atcaatcagc
    11521 gccttatcga aatggcggat accagtctgc tggtattgcc ggagtttctg ggattattgc
    11581 tgctgttgat ggcagtcact ctcggatcgc aactggcgct caccactttg gggcatcact
    11641 tcgtttaccg actgcgtagt gaatttatca agcggattct ggatacccac gtcgagcgca
    11701 ttgaacaact cggtagcgcc tcgttgttgg cgggattaac cagcgatgtg cgcaatatca
    11761 ccattgcttt tgtgcgtctt ccggaactgg tgcaggggat cattctcact atcggttccg
    11821 cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt aacggctatc tggatggcga
    11881 tcaccatctg gggtggtttt gtactggtgg cgcgggtgta caaacatatg gcgaccctac
    11941 gtgaaaccga agacaagctg tacacggatt ttcaaactgt acttgaaggg cgcaaagagc
    12001 tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa cctctacatc cccgatgcgc
    12061 aagagtatcg ccatcatatt attcgcgcag acaccttcca tcttagtgcc gtgaactggt
    12121 caaacatcat gatgctgggc gctatcggcc tggtgttctg gatggcgaac agtctcggtt
    12181 gggctgatac caacgttgct gcgacctatt cgttgacgct tttattcctg cgtacgccgc
    12241 tgctttcggc ggttggcgca ttgccgacgc tgctgatggc gcaggtggcg tttaacaagc
    12301 tgaacaaatt cgcgctcgcg cctttcaaag cagagtttcc gcgcccgcag gcgtttccca
    12361 actggcaaac gctggagctg cgtaatgtga cgtttagcta tcaggataac gcgttttccg
    12421 ttggtccgat taatctcacc atcaaacgtg gcgagctact gtttctgatt ggtggtaacg
    12481 gtagtggaaa atcgacgctg gcgatgttgt tgacgggctt gtatcagcca caaagcggcg
    12541 aaatcttgct ggatggcaaa cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt
    12601 tttcggcagt gtttaccgat gtctggcttt ttgatcaact gctggggccg gagggtaaac
    12661 ccgctaaccc gcaactggtt gagaagtggc tggcgcagct gaaaatggct cataagcttg
    12721 agttaagcaa cgggcgtatt gttaacctga agttatcaaa agggcagaaa aaacgcgtgg
    12781 cgctgttgct ggcgctggca gaagaacgcg atattatcct gctggatgaa tgggcggcgg
    12841 atcaggatcc acacttccgt cgtgagtttt atcaggtgtt gctgccgctg atgcaggaga
    12901 tgggtaaaac tattttcgct atcagtcatg atgatcatta ctttatccac gccgaccgcc
    12961 tgctggaaat gcgcaatggg caacttagcg agctgacggg cgaagagcgc gatgccgctt
    13021 cgcgtgatgc cgttgcccgg acggcataat attacgacgc tgacctggcg gactgctgcc
    13081 gtcaggtcaa tatgctaaaa atcccttcat ttctaataac cctataatta atcaacgaaa
    13141 ttataatgtt tctaaaatta gaatataatt tataaacatt atttaaatgt tgttacttaa
    13201 gtgttacctt tgatgccgcg caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt
    13261 aacaagatga aaaaagtgac tgccatgctc ttctcgatgg ccgtggggct taatgccgtt
    13321 tcgatggcgg caaaagcgaa agcgtccgag gagcaggaaa ctgatgtact gttgattggc
    13381 ggcggcatta tgagcgccac gttggggacc tatttacgcg agctggagcc tgaatggtcg
    13441 atgaccatgc tggagcgcct ggagggtgtc gcgcaggaga gttcgaacgg ctggaataac
    13501 gccggaaccg ggcattctgc actgatggaa ctgaactaca ccccgcaaaa cgccgatggc
    13561 agtatcagta ttgaaaaagc agtcgccatt aacgaagcat ttcagatttc ccgccagttc
    13621 tgggcgcacc aggtcgagcg cggcgtgctg cgtactccgc gttcatttat caataccgtt
    13681 ccgcatatga gctttgtctg gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc
    13741 gcgttgcaac aaagctcgct gtttcgcggt atgcgttact ctgaagatca cgcgcagatc
    13801 aaagagtggg caccgttagt gatggaaggg cgcgatccgc aacagaaagt ggcagccacg
    13861 cgtacggaaa ttggtaccga tgtgaactac ggcgagatca cccgccagtt aattgcttcc
    13921 ttgcagaaga aatctaactt ctcgctgcaa ctcagcagcg aagtccgcgc cctaaagcgt
    13981 aatgacgata acacctggac cgttaccgtt gccgatctga aaaatggcac tgcacagaac
    14041 attcgtgcca aatttgtctt tatcggcgcg ggcggtgcgg cgctgaagct gttacaggaa
    14101 tcggggattc cggaagcgaa agactacgcc ggtttcccgg tgggcggaca gttccttgtt
    14161 tcggaaaacc cggacgtggt taatcaccat ctggcgaagg tttacggtaa agcatccgtt
    14221 ggcgcaccac cgatgtcggt tccgcatatc gatacccgcg ttctggacgg taaacgcgta
    14281 gtgctgtttg ggccatttgc caccttctca accaaattcc tcaaaaacgg ttcattgtgg
    14341 gatctgatga gttccaccac cacctctaac gtgatgccga tgatgcacgt cgggctggat
    14401 aatttcgatc tggtgaaata tctggtgagt caggtgatgt tgagtgaaga ggatcgtttt
    14461 gaagcgttga aagagtacta tccgcaggcg aaaaaagagg actggcgttt gtggcaagcg
    14521 gggcagcgtg tgcagattat caagcgtgat gccgataaag gtggtgtact gcgtctgggt
    14581 actgaagtgg tcagtgacca gcaagggacc attgccgcgc tcctgggagc atcgccaggg
    14641 gcgtcaaccg ccgcgccgat tatgttggat ctgctggaaa aagtatttgg cgatcgcgtt
    14701 tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc cgtcttatgg acgcaagctg
    14761 aacggtgatg tagcggcaac agaacgcgag ttgcagtaca ccagcgaagt gctggggctg
    14821 aaatacgaca agccgcaggc agcagatagt acgccaaaac cgcagttgaa accgcaaccc
    14881 gttcaaaaag aagtggcgga tattgcgttg taatgatgtg ccacatccgg catggtatgc
    14941 cggatgtggc gtatactgat aagacgcgcc agcgtcgcat caggcaaccg gctcgggcgt
    15001 tagatgttgg atgcggcgta accgccttat ccaaccaacg gggtgccgta cttcacaaca
    15061 gtttagcgaa cttctgcgtt gtcaattttc tcttccgcct tccagacgcg gtatttcaca
    15121 tctacattgt ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca
    15181 gcatcgccca gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc
    15241 atcgtggaaa ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc
    15301 agcgttttgc tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc
    15361 tgaccgatta acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc
    15421 acctgacgct tcatcccttt ttcagcttgt ggataaggcg cgattttttc cagtggctgg
    15481 acgctttctg ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac tgcaggtaga
    15541 atggtcttca tttttttgct ttccttcttc atttataggt tttcaccgat gatgtcgctg
    15601 aaggttaaca tattcataag atttattaat ttgctttatg aataacttaa agaatattca
    15661 gacaatttta cctgctcttt tagtgttaaa ttctgatgag agaacaaacc agcagaaata
    15721 tctctggtga attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag
    15781 cccgtttttg ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg
    15841 gggtatgcat ctttgacaca tcctttaata tcttagcggt tataaaaatg gcttattaat
    15901 tatgcggttt atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt
    15961 gaagattgat gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg
    16021 tatccgtccg ccctggtccg gtgatgaatc tcattttctg acccattgca cccgttgcga
    16081 cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag
    16141 cgttaatttc aaaaataacg agtgcagctt ctgttacgct tgcgcgcagg cttgccccga
    16201 atcgctattt tctccgcgcc acaccagggc ctgggatttg cagttcacca ttggggacgc
    16261 gtgcctggcg tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc
    16321 aattatcttt cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg
    16381 caacggatgt ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct
    16441 ccatgcacac taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt
    16501 cagacatcag cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc
    16561 cgagcggtca gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca
    16621 ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg
    16681 aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc
    16741 cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt
    16801 tggtcagcag gaagccatca aatgggataa agcgccgtgc cgtttctgcg gtactggttg
    16861 cggcgttctg gtcggaacgc agcagggacg tgtggtggcc tgtcagggcg acccagacgc
    16921 accggttaac cgtggcctga actgcattaa gggctatttc ctgcccaaga tcatgtacgg
    16981 taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg
    17041 cgaatttacc ctaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac
    17101 cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat
    17161 ctgggaaggt tatgccgcgt ccaagctgtt taaagcgggc ttccgttcga acaacatcga
    17221 cccgaacgcg cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat
    17281 ggatgagccg atgggctgct atgacgacat cgagcaggct gacgcgtttg tgctgtgggg
    17341 cgcaaacatg gcggagatgc acccgatcct ctggtcgcgc atcactaacc gtcgtctctc
    17401 taatcagaac gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc
    17461 ggataacggc atcatcttta cgccacagtc tgacctggtg atcctgaact acatcgccaa
    17521 ctatatcatt caaaacaatg cgataaatca ggacttcttc agcaaacacg ttaacctgcg
    17581 caaaggggcg acggacattg gctacggttt acgtccgacc catccgctgg aaaaagcagc
    17641 gaagaatcca ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt
    17701 tgccgagtat acgctggaaa aaactgccga aatgaccggc gtgccgaaag accagttaga
    17761 acaactggcg cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg
    17821 cttcaaccag catactcgtg gcgtgtgggc taacaacctg gtctacaacc tgcacctgct
    17881 gaccggcaaa atttcccagc cgggttgcgg tccgttctcc ctgaccgggc aaccttctgc
    17941 gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt
    18001 gaccaacgag aaacaccgtg atatctgcga gaagaagtgg aatatcccga gcggcaccat
    18061 tccggcgaaa atcggtctgc acgcggtggc acaagaccgt gcgctgaaag acggcaaact
    18121 gaatgtttac tggaccatgt gtaccaacaa catgcaggcc gggccgaaca tcaatgaaga
    18181 gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac
    18241 agtcagtgcg ctggccgccg acttgatcct gccgaccgca atgtgggtag agaaagaggg
    18301 cgcttacggt aacgccgaac gccgtactca gttctggcgt caacaggtac aggcaccggg
    18361 cgaagcgaaa tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga
    18421 agtatggccg gaagatctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga
    18481 agtgctgtat gccacaccgg aagtgagcaa attcccggta tccgaactgg cggaagatca
    18541 gctgaacgat gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta
    18601 cgcatggttt ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc
    18661 gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg
    18721 taacgacccg tacgtgaaag cgggcgaagg ctataagttc tacggcaaac cggatggcaa
    18781 agcggtgatc ttcgcactgc cattcgaacc ggcggcggaa gcaccggatg aagagtacga
    18841 cctgtggctc tctaccggac gcgttctgga gcactggcac accggcagta tgactcgccg
    18901 tgtaccagaa ctgcaccgcg ccttcccgga agcggtcctg tttattcacc cgctggatgc
    18961 gaaagcgcgc gatctgcgtc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt
    19021 gatctcgatt gtcgaaacgc gcggtcgtaa ccgtccgccg cagggcctgg tgtacatgcc
    19081 gttcttcgac gccgcacagc tggttaacaa actaacgcag gacgcgaccg atccgctctc
    19141 gaaagagacg gacttcaaga agtgcgcagt caaactggag aaggtgtaag ccattatgtc
    19201 ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg cgcgatgttg ttcgcacagc
    19261 aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc
    19321 tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt
    19381 gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc
    19441 ctctggtctg tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg
    19501 tgaggacatt ccgtgcgcca aagtgtgccc gagcggtgcg ctggatcgtg agatcgaatc
    19561 gatcgacgac gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt
    19621 tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac
    19681 cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca
    19741 cagagacgcc tgtaccggtt gcggtaagtg cgaaaaagtg tgcgtgctgg aacaaccggc
    19801 aatcaaggtg ttaccgctgt cactggctaa aggggagtta ggtcaccatt accgcttcgg
    19861 ctggctggag gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga
    19921 aaaaaggctg gtggcgcagt caccgttggc tggtgttacg tcgcctttgt cagttcttcg
    19981 tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg gatcttacac ggtaactaca
    20041 gcagtagcct gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc
    20101 ttgccagcgg tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc
    20161 tctacgccct ggcaggaaag cgattatttt gcagctgggt ctgcccgctg aatccgatta
    20221 ccgacttagc gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc
    20281 gccacattcg ctacgtgctg ttggtggtca ttctgctggg atcggcctta actggcacgc
    20341 tcatctggga atggatcaac cccgtatctc tgatggggcg cagcctggta atgggcttcg
    20401 gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg
    20461 gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg
    20521 tgattaccgt tgcggccacc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg
    20581 tttgcccgga accgcgtgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg
    20641 tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat
    20701 ttacaataac tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa
    20761 agcgctgtgt caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc
    20821 taatggcgtc gattttagcc agtcgccgga agtatccggg acacaggaag gggccattcg
    20881 gatgccaaaa gagcaggaac ggatgccgct gaactatgtg aatcagccgc caatgatccc
    20941 gcatagcgtt gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg
    21001 tgtcgaaagt tatcgcacca ctggcgcgcc gcgcattagt cctactcact ttatggacag
    21061 cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt
    21121 accgcaggcc gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg
    21181 gaaataagag gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg
    21241 gaaatggtgg cgtaccccca gccgtctggc gctggggacg ctgctgttga tcggttttgt
    21301 tggcggcatc gtcttctggg gtggctttaa caccgggatg gaaaaagcca ataccgaaga
    21361 gttctgcatt agctgccacg aaatgcgcaa cacggtgtat caggaataca tggattccgt
    21421 gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga
    21481 gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt
    21541 tggcgttatt gacacgccgc agaaatttga agctcatcgt ctgacgatgg cacagaatga
    21601 gtggcggcgc atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta
    21661 tatggataca accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga
    21721 tgggcaaacc tgtattgatt gccataaagg gatagcgcac aagctgcccg atatgcgtga
    21781 agtcgagcca ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact
    21841 ttgtgagcgg gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga
    21901 gtgggtacaa atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac
    21961 ggggttgtct cgccctgacg caggcgaggt tctctggcaa gggcagccct tgcatcaggt
    22021 acgcgacagc taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg
    22081 gctgacggcg ttagaaaatc tgcactttta tcatcgcgat ggcgataccg cacaatgtct
    22141 ggaagccctg gcgcaggccg ggcttgccgg attcgaagat attcctgtaa atcagctctc
    22201 ggccgggcaa caacgccgcg tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg
    22261 gatcctcgac gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg
    22321 tatggcgcag catacggagc agggggggat tgtgattctg actacccacc agccgctcaa
    22381 cgttgctgaa agtaaaattc gccgcatttc actgacgcaa acgagggccg catgatgttc
    22441 tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc atagcgccga aatcgccaac
    22501 ccgctgtggt tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg
    22561 caactgctgg cgcgtattgc accgggcatt atctgggttg ctgcgctgct ttcatccttg
    22621 ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg
    22681 ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta
    22741 accggtctgc cgttactcat cctttcgcca ctggtagcaa tgctactggg aatggatgtt
    22801 tatggctggc aagtgatggc gctgacgctg ctgctgggaa cgcctacgct tggctttctc
    22861 ggtgcaccgg gtgtggcgtt gacagtggga cttaagcgcg gtggtgtgct gctcagcata
    22921 ctggtgttac cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct
    22981 tccatgcatt tgcccgttga cgggtatctg gcaattttag gcgcgttgct ggcaggcacc
    23041 gcgacattaa gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg
    23101 attactgatt tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg
    23161 cgatcccacc acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca
    23221 gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc
    23281 agcagggaaa tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg
    23341 gtatttatgc atcaatggcg gtggcggcgt ttattggcct tgtctggcag atgaaaatgg
    23401 ccaacctggc ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg
    23461 ttaccggctc tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc
    23521 tgacttctga actggtgctg ctgtttttgt atgtgggtgt gattgccctg tggcacgcct
    23581 tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga
    23641 atctgccgat tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc
    23701 ggatgcagca aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct
    23761 tcctgctcct gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg
    23821 aaaaacgccg tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccttgc
    23881 atttgcttcc tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc
    23941 ggtggtgatg accgttattc cgctggtggt tttggtcgtg cactcggtga tgcagcatcg
    24001 cgcaattctg tgtggtgtgg agcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca
    24061 acagcaggag gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgctgtg
    24121 ttggcaggtt tggcgctgac catcggtctg gtgctgtatg cgctgcgctc gaatatcgat
    24181 ctcttttata cgccggggga aattctctac ggcaagcgtg aaactcagca aatgccggaa
    24241 gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg gtagtgtgca gcgcgatccc
    24301 aattcgctga aagtgacctt caccatttac gacgctgaag gctcagtgga tgtctcttac
    24361 gaaggcattt tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgagctg
    24421 gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga aacacgacga aaactacacg
    24481 ccgccagaag ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag
    24541 gacccagcat catgatgccg gaaattggaa acgggctgct gtgcctggcg ctgggaattg
    24601 cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg
    24661 cgtcttcccg cttgtttgcc tggatgctgt ttatgtctgt ggctggcgca tttctggtgc
    24721 tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata
    24781 cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg cgcgcatgaa ggttcgcttc
    24841 tgctgtgggt gctgctgatg agcggctgga cctttgcggt ggcgattttt agtcagcgta
    24901 ttccgctgga tattgtggcc cgtgtactgg cgataatggg gatggtcagc gtcggctttt
    24961 tgctgttcat tctctttacc tctaacccgt tctcacgcac gctgccgaac ttcccaattg
    25021 aagggcgcga tcttaacccg ctgttacagg atccggggct gatcttccat ccgcctctgc
    25081 tttatatggg gtacgtgggt ttctcggtgg cgtttgcttt tgccattgcc attgcttctt
    25141 tgctgagcgg gcgtctggac agcacttatg cgcgttttac tcgcccgtgg acgctggcgg
    25201 cgtggatttt cctgacgctc ggcattgtgc tcggttccgc atgggcctat tacgaactcg
    25261 gctggggggg ctggtggttc tgggatccgg tagaaaacgc ctcgtttatg ccgtggctgg
    25321 tggggactgc gctgatgcac tcactggcgg tcactgaaca acgcgccagc ttcaaagcgt
    25381 ggacattact gctggcaatc agtgccttct cgttgtgtct gctggggact ttcctggtgc
    25441 gttccggcgt gctggtatcg gtacacgcgt ttgcctctga tccggcacgc ggtatgttta
    25501 tcctcgcctt tatggtactg gtgattggtg gttcgctgct gctgtttgcc gcgcgtggac
    25561 acaaagttcg ctcacgcgta aacaatgcgc tgtggtcgcg ggaatctctg ctgttagcga
    25621 acaacgtttt gttggtcgcc gcgatgctgg tggtattact ggggacgctg ctgccgctgg
    25681 tgcacaagca actgggactg ggcagtattt cgattggcga accgttcttc aacaccatgt
    25741 ttacctggct gatggtgccg tttgcgctgc tgcttggtgt cggtcctctg gtgcgctggg
    25801 ggcgcgatcg cccacgtaaa atccgcaatt tattgattat cgccttcatc tcaacgctgg
    25861 tgctgtcgct gctgctgccg tggctgttcg aaagcaaagt tgtggcgatg acggtgctcg
    25921 gcctggcaat ggcctgctgg attgctgtgc tggcaattgc ggaagctgcg ctgcgtattt
    25981 cacgtggcac gaaaaccacc ttcagttatt ggggaatggt ggcggctcac ctggggctgg
    26041 cagtgacaat tgttggtatt gcctttagcc agaactatag cgttgagcgt gatgtgcgca
    26101 tgaagtccgg cgatagcgtc gatattcatg aatatcgctt caccttccgt gatgtcaaag
    26161 aggtgactgg cccgaactgg cgcggcggtg tggcgactat tggcgtaacg cgagagggta
    26221 aaccggaaac ggtgctgtat gcggaaaaac gttattacaa cactgccggg tcgatgatga
    26281 ccgaagcggc gattgacggc ggcatcacgc gtgacctgta cgccgcgctc ggtgaagagc
    26341 tggaaaacgg cgcgtgggcc gtgcgtcttt actacaaacc gtttgttcgc tggatttggg
    26401 cgggcgggct gatgatggcg ttgggcggac tgctgtgtct gtttgatcct cgctatcgta
    26461 agcgcgtgag tccgcaaaaa actgcgccgg aggctgtatg aagcgcaaag tattgttaat
    26521 tccgttgatt atcttcctgg cgattgccgc ggcgctgctg tggcagctgg cgcgtaatgc
    26581 cgaaggggat gatccgacca atctggaatc ggcgctcatt ggcaagccag tgccgaagtt
    26641 tcgcctcgaa tcactggaca atccggggca gttttatcag gcggatgtgc tgactcaggg
    26701 caaacctgtg ctgcttaacg tttgggctac ctggtgtccg acctgccgtg cggaacatca
    26761 atatctgaat cagctttctg cgcagggcat tcgcgtggtc ggcatgaact ataaagacga
    26821 tcgccagaag gcaataagct ggctgaaaga gctgggcaat ccttacgcgc taagcctgtt
    26881 tgatggcgat ggcatgttag ggctggatct cggtgtctat ggcgcgccag aaacgttcct
    26941 catcgacggc aacggcatca ttcgctatcg ccatgcgggc gatctcaatc ctcgcgtctg
    27001 ggaagaagag atcaagccgc tgtgggagaa atacagtaag gaggccgcac aatgaggttt
    27061 ttattgggcg tgctgatgct gatgatctcc ggctcagcgc tggcgaccat cgacgtgttg
    27121 cagtttaagg atgaagcgca ggagcagcag ttccgccaac tcactgaaga actacgctgc
    27181 ccgaaatgcc agaacaacag cattgccgat tccaactcga tgattgccac cgacctgcgc
    27241 cagaaagtgt atgaactgat gcaggaaggt aaaagtaaaa aagagattgt cgattatatg
    27301 gtggcgcgtt acggcaactt cgtcacttac gatccgccgt taacgccgct gaccgtgctg
    27361 ctgtgggtgc ttccggtagt ggctattggc attggcggtt gggtcattta cgcccgttcg
    27421 cggcgtcggg tacgcgtggt gccggacgcg tttcctgaac aaagcgtgcc ggaaggtaag
    27481 cgtgccggat atattgttta tctgccgggt attgtggtgg cgttaattgt ggctggcgtc
    27541 agctactacc agactggcaa ttatcagcag gtgaaaatct ggcagcaggc cacggcacag
    27601 gctccggcgt tgctggacag ggcgctggat ccgaaagccg atccgctcaa cgaagaagag
    27661 atgtcgcgtc ttgcgctggg gatgcgtact caactgcaaa aaaatccggg agatatagaa
    27721 ggctggatta tgttgggccg cgttggcatg gcgctgggta acgccagtat cgccaccgat
    27781 gcatacgcta ctgcgtatcg cctcgatccg aaaaacagtg atgccgcact gggttatgct
    27841 gaagcgttga cacgttcatc tgatcccaac gacaaccgcc tcggtggtga actgctgcgc
    27901 cagttggtga gaacggacca tagcaatatc cgtgtgttaa gcatgtatgc gtttaatgcc
    27961 tttgagcagc agcgatttgg cgaagccgtt gccgcgtggg agatgatgtt gaaactctta
    28021 tctgccaacg atactcgccg tgcggtgatt gaacgtagta tcgcgcaggc gatgcaacat
    28081 ttgtcgccgc aggagagtaa ataaaaagag gcccgatggt gatgccatcg ggctattttt
    28141 attgtgcccc gcgttgttgc aggaacagaa tggtggctgc cacgcgtgag cggacattga
    28201 gtttgcgcag cagattgcga atatgtactt ttactgtctg ctctgaaata ttcaacaccg
    28261 aggcaatctg tttatttgac agcccctgtg ccagctcgtg cagaacatcc agctcgcgct
    28321 ccgtcagcac gctgaaggga tcttcttccg cgccaaacat ttcacgttca cgtaagtact
    28381 gattgacgcg ttcgctaaag actttgctgc ctttcgctcc agcacgaatt gcttccagca
    28441 atacttccgg gtcgctgtct ttcaacagat aaccgtctgc gcctgcgtct atcagcgcaa
    28501 agacatcgct gg
//
LOCUS       DAJTYD010000035        27306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTYD010000035 DAJTYD010000000
VERSION     DAJTYD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.035"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..520
                     /locus_tag="OO585_001390"
     CDS             <1..520
                     /locus_tag="OO585_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6629027.1"
                     /translation="KIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEIIN
                     KKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGN
                     DGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGL
                     MPLIRRRTMLQL"
     gene            886..1953
                     /locus_tag="OO585_001391"
     CDS             886..1953
                     /locus_tag="OO585_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6629028.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1950..4685
                     /gene="rbbA"
                     /locus_tag="OO585_001392"
     CDS             1950..4685
                     /gene="rbbA"
                     /locus_tag="OO585_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6629029.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4685..5809
                     /locus_tag="OO585_001393"
     CDS             4685..5809
                     /locus_tag="OO585_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6629030.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5882..6156
                     /locus_tag="OO585_001394"
                     /pseudo
     CDS             5882..6156
                     /locus_tag="OO585_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6153..6512
                     /locus_tag="OO585_001395"
     CDS             6153..6512
                     /locus_tag="OO585_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6629031.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6632..7033)
                     /gene="nikR"
                     /locus_tag="OO585_001396"
     CDS             complement(6632..7033)
                     /gene="nikR"
                     /locus_tag="OO585_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6629032.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7039..7845)
                     /gene="nikE"
                     /locus_tag="OO585_001397"
     CDS             complement(7039..7845)
                     /gene="nikE"
                     /locus_tag="OO585_001397"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6629033.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7842..8606)
                     /gene="nikD"
                     /locus_tag="OO585_001398"
     CDS             complement(7842..8606)
                     /gene="nikD"
                     /locus_tag="OO585_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6629034.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8606..9439)
                     /gene="nikC"
                     /locus_tag="OO585_001399"
     CDS             complement(8606..9439)
                     /gene="nikC"
                     /locus_tag="OO585_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6629035.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9436..10380)
                     /gene="nikB"
                     /locus_tag="OO585_001400"
     CDS             complement(9436..10380)
                     /gene="nikB"
                     /locus_tag="OO585_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6629036.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQFFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10380..11954)
                     /gene="nikA"
                     /locus_tag="OO585_001401"
     CDS             complement(10380..11954)
                     /gene="nikA"
                     /locus_tag="OO585_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6629037.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12065..12652)
                     /gene="acpT"
                     /locus_tag="OO585_001402"
     CDS             complement(12065..12652)
                     /gene="acpT"
                     /locus_tag="OO585_001402"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6629038.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12707..13756)
                     /locus_tag="OO585_001403"
     CDS             complement(12707..13756)
                     /locus_tag="OO585_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6629039.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVTITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMIGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYACILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13888..15105
                     /locus_tag="OO585_001404"
     CDS             13888..15105
                     /locus_tag="OO585_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6629040.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15109..15666)
                     /gene="dcrB"
                     /locus_tag="OO585_001405"
     CDS             complement(15109..15666)
                     /gene="dcrB"
                     /locus_tag="OO585_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6629041.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15739..16404)
                     /locus_tag="OO585_001406"
     CDS             complement(15739..16404)
                     /locus_tag="OO585_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6629042.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16625..16870
                     /gene="tusA"
                     /locus_tag="OO585_001407"
     CDS             16625..16870
                     /gene="tusA"
                     /locus_tag="OO585_001407"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6629043.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16972..19170)
                     /gene="zntA"
                     /locus_tag="OO585_001408"
     CDS             complement(16972..19170)
                     /gene="zntA"
                     /locus_tag="OO585_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6629044.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19244..19870)
                     /locus_tag="OO585_001409"
     CDS             complement(19244..19870)
                     /locus_tag="OO585_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6629045.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20011..20370
                     /locus_tag="OO585_001410"
     CDS             20011..20370
                     /locus_tag="OO585_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6629046.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20373..20642)
                     /locus_tag="OO585_001411"
     CDS             complement(20373..20642)
                     /locus_tag="OO585_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6629047.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20632..21228)
                     /gene="rsmD"
                     /locus_tag="OO585_001412"
     CDS             complement(20632..21228)
                     /gene="rsmD"
                     /locus_tag="OO585_001412"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6629048.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21378..22871
                     /gene="ftsY"
                     /locus_tag="OO585_001413"
     CDS             21378..22871
                     /gene="ftsY"
                     /locus_tag="OO585_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6629049.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22874..23542
                     /gene="ftsE"
                     /locus_tag="OO585_001414"
     CDS             22874..23542
                     /gene="ftsE"
                     /locus_tag="OO585_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6629050.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23535..24593
                     /gene="ftsX"
                     /locus_tag="OO585_001415"
     CDS             23535..24593
                     /gene="ftsX"
                     /locus_tag="OO585_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6629051.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24838..25692
                     /gene="rpoH"
                     /locus_tag="OO585_001416"
     CDS             24838..25692
                     /gene="rpoH"
                     /locus_tag="OO585_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6629052.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25963..27066
                     /gene="livJ"
                     /locus_tag="OO585_001417"
     CDS             25963..27066
                     /gene="livJ"
                     /locus_tag="OO585_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6629053.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
BASE COUNT         6446 a         7088 c         7620 g         6152 t
ORIGIN      
        1 caaaataatg gcaaatcatc atgggataat ttttgatgag cagcgaaaac gttacattat
       61 aggatcgata acagtctcta ttgatgaaat gaccaatgct ctcgatccgg tcgaattagc
      121 ggaaattatt aataaaaagc accatgctgt aattaatggc aaaaagttta aaaatagccg
      181 ggctatctct tgtcgatcct ttaatcgtta tttttctgtt tcgacagatt acagacctaa
      241 acttgaagca ctattagcat gctcacaaat gttaggtata aatcaagttg ttgcacataa
      301 cggtaatgat ggaagagaat gtataggtga aacggggaca gttttaggtc ttaacgcccg
      361 tgacagtaaa catgctggaa gaatgttcag catgcataat tgccaaatta accctggtgc
      421 agggcctgag ataaccactc cctggaagtc ttaccagcat gaaaaaaaca gaaatggact
      481 tatgcctttg attagaaggc gcacaatgtt acagctctga accatagaca acatgaatat
      541 taatgttact atctggttga gactgttgat aaaacgtaaa aaggaatgct ctgttcctgc
      601 aactgaacag gcgtgaaacg cgatccaaca ggatggatac cgttttgtca gccagtttta
      661 tagatcacac ttatgagtat gcgtgtttca tttacggcca ggaatgctga tgatgacaac
      721 attactaccc gttttcacta agccttcccc gttggcgctc aatgctctgc gcgctggtcg
      781 tatttgtcgt ttccttctta tcccggatgg aagaatccgc tgaacgtcgt tcggcggcag
      841 ttgtaacacg cgtcgtttgc ttacagggat tatggcgggc cgattatgga taagagtaag
      901 cgccatctgg cgtggtgggt tgtcggggca ctggcggtgg ctgccgtcgt ggcgtggtgg
      961 ctgttgcgcc cggcgggtgt gccggaaggc tttgctgtca gcaatgggcg cattgaagcg
     1021 acggaagtgg atatcgccag caaaattgcc gggcgtatcg acaccattct ggtgaaagaa
     1081 ggccagtttg ttcgcgaagg tgaagtgctg gcgaagatgg atactcgtgt gttgcaggaa
     1141 cagcgactgg aagccatcgc gcaaatcaaa gaggcacaaa gcgccgttgc cgccgcgcag
     1201 gctttgctgg agcaacgaca aagcgaaact cgcgccgcgc agtcgctggt taatcaacgc
     1261 caggcagaac tggactccgt agcaaaacgt catatgcgtt cccgttcact ggcccaacga
     1321 ggggctattt ctgcgcaaca gctggatgac gaccgcgccg ccgctgagag cgcccgagct
     1381 gcgctggaat cggcaaaagc tcaggtttct gcttctaaag ccgctataga ggcggcacgt
     1441 accaatatca ttcaggcgca aacccgcgtc gaagcggcac aagccactga acggcgcatt
     1501 gccgcagata tcgatgacag cgaactgaaa gccccgcgtg acggacgcgt gcagtatcgg
     1561 gttgccgagc caggggaagt gctggcggca ggcggtcggg tgctgaatat ggtcgatctc
     1621 agcgacgtct atatgacttt cttcctgcca accgaacagg cgggcacgct aaaactgggc
     1681 ggtgaagcac ggctgatcct cgatgccgcg ccagatctgc gtattccggc gaccatcagt
     1741 tttgtcgcca gcgtcgccca gttcacgcca aaaaccgtcg aaaccagcga tgaacggctg
     1801 aaactgatgt tccgcgtcaa agcgcgtatc ccaccggaat tactccagca gcatctggaa
     1861 tatgtcaaaa cgggcttgcc gggcgtagcg tgggtgcggg tgaatgaaga acttccgtgg
     1921 cctgacgacc tggtggtgag gttgccgcaa tgacgcatct ggaactggtt cccgtcccgc
     1981 ctgtcgcgca actggcgggc gtgagccagc attatggaaa aaccgttgcg ctgaacaata
     2041 tcactctcga tattccggcc cgctgtatgg tcgggctgat tggcccggac ggcgtcggga
     2101 agtcgagttt gttgtcgttg atttccggtg ctcgtgtcat tgagcagggc aatgtgatgg
     2161 tgctgggcgg cgatatgcgc gacccgaagc atcgccgcga cgtctgcccg cgcatcgcct
     2221 ggatgccgca ggggctgggc aaaaacctct accacacctt gtcggtgtat gaaaacgtcg
     2281 attttttcgc ccgcctgttc ggtcacgaca aagcggagcg ggaagtgcga attaatgagc
     2341 tgctgaccag cacggggtta gcaccgtttc gcgatcgtcc ggcagggaaa ctctccggcg
     2401 ggatgaagca aaaacttggg ctgtgctgcg cgttaatcca cgacccggaa ctgttgatcc
     2461 ttgatgagcc aacaacgggg gttgacccgc tctcccgcgc ccagttctgg gatctgatcg
     2521 acagtattcg ccagcggcag agcaatatga gcgtgctggt cgccaccgcc tatatggaag
     2581 aggccgaacg cttcgactgg ctggtagcga tgaatgccgg agaagtgctg gcaaccggca
     2641 gcgccgaaga gctgcggcag caaacgcaaa gcgccacgct ggaagaagca tttattaatc
     2701 tgttaccgca agcgcaacgc caggcgcatc aggcggtagt gatcccaccg tatcaacctg
     2761 aaaacgcaga gattgccatc gaagcgcgcg atctgaccat gcgttttggt tccttcgttg
     2821 ccgttgatca cgtcaatttc cgcattccac gcggggagat ttttggattc cttggttcga
     2881 acggctgcgg taaatccacc accatgaaaa tgcttaccgg actgctgccc gccagcgaag
     2941 gtgaggcgtg gctatttggg caaccggttg atccaaaaga tatcgatacc cgccgtcgtg
     3001 tgggctatat gtcgcaggcg ttttcgctct ataacgaact caccgtgcgg caaaacctgg
     3061 agttacatgc ccgtttgttt cacatcccgg aagcggaaat tcccgcgcga gtggctgaaa
     3121 tgagcgagcg ttttaagctc aacgacgttg aagatgttct gccggagtca ttgccgctcg
     3181 gcattcgcca gcggctttcg ctggcggtgg cggtgattca tcgcccggag atgttaatcc
     3241 tcgatgagcc tacttctggt gtcgatccgg tggcgaggga tatgttctgg cagttgatgg
     3301 tcgatctctc gcgccaggac aaagtgacta tcttcatctc cacccacttt atgaacgaag
     3361 cggaacgttg cgaccgcatc tcactgatgc acgccggaaa agtgcttgcc agcggtacac
     3421 cgcaggaact ggttgagaaa cgcggagccg ccagtctgga agaggcattt atcgcctatt
     3481 tgcaggaagc ggcagggcag agcaacgaag ccgaagcgcc gcccgtggta cacgacacca
     3541 cccacgcgcc gcgtcaggga tttagcctgc gccgtctgtt tagctacagc cgccgcgaag
     3601 cgctggaact gcgacgcgat ccagtacgtt cgacgctggc gctgatggga acggtgatcc
     3661 tgatgctgat aatgggttac ggcatcagta tggatgtgga aaacctgcgc tttgcggtgc
     3721 tcgaccgcga ccagaccgtc agtagccagg cgtggacact caacctctcc ggttcccgtt
     3781 actttatcga acagccgccg ctcaccagtt atgacgagct tgatcgtcgg atgcgtgcgg
     3841 gcgatatcac ggtggcgatt gagatcccgc ccaatttcgg gcgcgatatc gcgcgtggta
     3901 cgcctgtgga actcggcgtc tggatcgacg gagcgatgcc cagtcgtgcc gaaacggtaa
     3961 aaggttacgt gcaggcaatg caccagagtt ggcttcagga tgtggcgagc cgacaatcga
     4021 cacccgccag ccaaagcggg ctgatgaaca ttgagacgcg ttatcgctat aacccggacg
     4081 taaaaagcct gccagcaatt gttccggcgg tgatcccgct tctgctgatg atgatcccgt
     4141 caatgctaag cacccttagc gtggtgcggg aaaaagagct tgggtcgatt atcaaccttt
     4201 acgtgacccc caccacgcgt agtgaatttt tgcttggtaa acagttgcca tacatcgcgc
     4261 tggggatgct gaactttttc ctgctctgcg gcctgtcggt gtttgtgttt ggcgtaccgc
     4321 ataaaggcag tttcctgacg ctcaccctgg cggcgctgct gtatatcatc attgccaccg
     4381 gaatggggct gctgatctcc acctttatga aaagccagat tgccgccatt ttcggaacgg
     4441 cgattatcac gttgatcccg gcgacacagt tttccgggat gatcgatccg gtagcttcgc
     4501 tggaagggcc tggacgttgg atcggcgagg tttacccgac cagtcatttt ctgactatcg
     4561 cccgcgggac gttctcgaaa gcgctggatc tgactgatat gtggcaactt tttatcccgt
     4621 tgctgatagc catcccgctg gtgatgggct taagtatcct gctgctgaaa aaacaggagg
     4681 gatgatgcgc catttacgca atatttttaa tctgggtatc aaagagttgc gcagtctgct
     4741 cggtgataaa gcgatgctga cgctgattgt cttctcgttt acggtgtcgg tgtattcgtc
     4801 agcgaccgtt acgccaggat cgttgaacct cgcgccgatc gccattgccg atatggatca
     4861 atcgcagtta tcgaaccgga tcgttaacag cttctatcgt ccgtggtttt tgccaccgga
     4921 gatgatcacc gccgatgaga tggatgccgg actggacgcc ggacgctata ccttcgcgat
     4981 aaatattccg cctaattttc agcgtgatgt cctcgccgga cgccagccgg atattcaggt
     5041 gaacgtcgat gccacgcgca tgagccaggc atttaccggc aatgggtata tccagaatat
     5101 tatcaacggt gaagtgaaca gctttgtcgc gcgctaccgt gataacagcg aaccgttggt
     5161 atcgctggaa acccggatgc gctttaaccc gaaccttgat cccgcgtggt ttggcggggt
     5221 gatggcgatc atcaacaaca ttaccatgct ggcgattgta ttgaccggat cggcgctgat
     5281 ccgcgagcgt gaacacggca cggtggaaca cttactggtg atgccgataa cgccgtttga
     5341 gatcatgatg gcgaagatct ggtcgatggg gctggtggtg ctggtggtat cgggattatc
     5401 gctggtgctg atggtgaaag gtgtactggg cgtaccgatt gaaggctcga tcccgctgtt
     5461 tatgctgggc gtggcgctca gtctgtttgc caccacgtca atcggcattt ttatggggac
     5521 gatagcgcgt tcaatgccgc aactggggct gctggtgatt ctggtgctgc tgccgctgca
     5581 aatgctttcc ggtggatcca cgccgcgcga aagtatgccg cagatggtgc aggacattat
     5641 gctgaccatg ccgacgacac actttgttag cctcgcgcag gccattctct accggggtgc
     5701 cggattcgaa atcgtctggc cgcagttcct gacgctgatg gcaattggcg gcgcgttttt
     5761 caccattgcg ctgctgcgat tcaggaagac gattgggaca atggcgtaag gaagggaaac
     5821 ttgacttcat actgtgatgt catactattg atgtcataaa gtgaagtcat aaggacatgc
     5881 catgagaacc aaggtacagg ctttacggaa gaaacaaaaa atacattgga tcaaatattt
     5941 aaaactcctg ttcctcaagg gatcaagtgg tctgatatag agtcactggt taaagcatta
     6001 ggcggagaaa ttaaggaagg aagaggttcg cgttgtaagt tcatactaaa tatgagcgtt
     6061 gcgtgtttcc atcggcctca tccgtcgcca gataccgata aaggcgctgt agaaagcgtg
     6121 cgtgactggt tactaagtat aggggtaaaa ccatgatcaa actgaagacg ccaaactcta
     6181 tggagattgc cgggcaacca gctgttatta cctacgtgcc agaactgaat gcatttcgtg
     6241 gcaaattcct gggcttgtca ggttattgtg attttgtctc tgacagcatt caggggctgc
     6301 aaaaagaagg ggaactttcc ctgctagagt atcttgagga ttgtaaagct gcgggtattg
     6361 agccgtatgc gcgtacagaa aagattaaga cctttacgtt acgctatcca gaatcgctga
     6421 gtgaacgcct taataatgcg gctgcgcagc aacaagtctc tgttaatacc tatattattg
     6481 aaacactgaa tgagcgtttg aatcatttat aaattttagc ggcgtacatt acgcagaata
     6541 tctgccttta caggatctat ctatgcaact gaacttaatt ttataaatta agttcagtta
     6601 atatcttttc tttcctcgtt tcagaaataa ctcaatcttc cttcggcaag cactgcaaat
     6661 gcccatgccg cacgccgcgc tgggcgataa cgtcatcggc aaaatgctgc acgtcgccca
     6721 tatcaccttt cagcacggcg atttccagac agtcgtcgtg attgatatgc acatgcagcg
     6781 tggcgacgga gagatcgtgg tgatggtgct gggtggagac aatgcggctg gctaagtcgc
     6841 gtttttcgtg ttcatacaca taggagagca ccgcgaaacc ttgcgtgccg tgctgctgag
     6901 tggcctcctg ggccagggcg ctacgcagaa tatcgcggat agcttcggaa cggttgttat
     6961 aaccacgacg ctggctcagg ctgtccagcg tctccagtaa atcgtcatca agcgtgatgg
     7021 tgactcgttg catttgcgtt aaaccttttc tgtggcgcga cggcgcacgg ggaatgcggg
     7081 taataccgcg ttttgtagca cacgtccggc gtcagaggaa aaggttaatt tatctcccac
     7141 cgcctgggtt tcgacgattt gtccgttgtc catcaccatc acccgctggc aaaagcgttc
     7201 tacaagacgt aagtcgtggg tgatgaacag gcaggcggtg ccaaactgtt gttgcagctt
     7261 tttcagcagg cgaatgacgc ccgcctgtaa cacgagatca aggttagaaa cggtttcatc
     7321 cagaatcagc aatttcggtt cgaccgccag cgcgcgtgcc aggcagacgc gctggagctg
     7381 gccgccgctt aactgcggtg ggcgtttgtc gagaacgctg tcatcgagat cgaccgcctt
     7441 cagcatttcg ctggcgcgcg ccagttgttc ggattttttc agtgatagca ggtggcgcat
     7501 cggttcacgc aggatctcgc gcacggtttt gcgcggattt acggcactga tggagtcctg
     7561 aaacaccatc tgaatatcac ggcggaaggc tttacgttgg gcgcgattaa gtttcgccag
     7621 cgattcgccg cgccagctaa tattcccttg tgagggagac tctaagccta ccagcagccg
     7681 cgcgagggtg ctttttccgc agccgctgcg tcccagcagg gcgacggttt cgccgctttt
     7741 cagggtcagg gaaacgttat tcagcaccgc ctgatgttga tgttttccgc taaatccacc
     7801 gtgggcatag tgatgggaaa ggccgctgac gttaagtaag gtcatgatgc cagctccata
     7861 ccgtagaggg cgagatgagc ggaaaccagg ctgcgcgtca ccgtatgttt gggggcgtta
     7921 aacagcgttt ctacatcgcc ctgttcgaca attttacctt gtgacatcac cgccacgtca
     7981 tccgccagac gcgccacaac gcccatatca tgggtgacca gcagcattcc cggcgcttgt
     8041 ttttgcataa tgctttccag cagatcgagg atgcgcgcct gtgctaccac gtcgaggtcg
     8101 gtggtcggtt catcggcgat gataaacggt gattcacaca gcaccgccat cgcaatcatc
     8161 atgcgctgca acatgccgcc gctcatctcg aacgggtaca gcttcagcac gcgcgcggcg
     8221 ttttccagcc ccaccgcttc tatggcagcg gtaagcgtag cgtcatcggc gggtttccct
     8281 aacgccaggc aggtttcccg cgcgtgggtg tgcatggtgt gtagcggatt aaaggcgctg
     8341 cgcgggttct gcatgatggt ggcaattttg atgccgcgca gggcgcaggg ataaaccgct
     8401 ttgccatcgg ctaaaatttc ccccgccgtc tggcgaacgc cagcgggcaa aatgcccagc
     8461 gtcgcggcgc aggttaatga tttcccgctg ccgctaccgc cgactaacgc cagcacgcgc
     8521 ccgcgttgca gggttaacga tacaccgtgt accagcggct gcgcggcctg tagcgcgata
     8581 ttacgtagtt caatctgttg cggcattagt gtgcgtgctc cgtcaccaga tgagggtcca
     8641 gatgatcgcg cagtgcgtca cccaccaggt taaaggccat cacgctgata aacagcgcca
     8701 gccccggcca gaacatttgc agcggctggg tccagatata ctggcgcgcg tcgttaatca
     8761 tcacgcccca ttcggcggtc ggcgcggtca caccgaggcc aaggaaagac atccccgaga
     8821 cgtgcagcat catatggccg atatccagcg ttgccagcac cagcagcgaa gggatcaccg
     8881 cgcctgccag atgatcgaca aacactcgca catggcccgc accggaaagc cgtgacgcca
     8941 gcacaaactc gcgttggcgt agtgaaatca ccaggctgcg caccatgcgt gcataccacg
     9001 cccagtgcga cagggcgatg gcgataatta cgttggtcag cccggtgccg agcacgccaa
     9061 ccataaagaa cgacagaatc gaggtcggga aggtcataaa catatcggcg acgcgcatgg
     9121 tggcctgatc aacgcgcccg ccaatcaacc cggcgctgcc gccaataacc agccctaatg
     9181 tcagcaccag cagcaggcag gccattaccg aaccgagcga cacgcgggtc gctgccatca
     9241 gccgcgagaa aatatcgcga cctaagtgat cggtgcccag ccagtgctgc gcatccggcg
     9301 aaagcaggtg cgacggcaaa tcaatcgcct gggggtcata cggcagccac cactggctgg
     9361 tgagcgcaat cagcgccagc agggcgataa tgatcagcgc caggcgtacc gaccagcggg
     9421 aagagaggaa aaagttcacg cgtgcgctcc ttcatgacga cgaatgcgcg ggtccagcgc
     9481 ggcgttgagc aaatcgacaa tcaaattaca gaccacaaaa accaccacca tcatcagcgt
     9541 aaagaactga atcaccggat agtcacggtt aaaaatcgcc gacaccgcat agcgcccgac
     9601 gccaggccag gcaaagatgt tttcgataat catcgtcccg ccaatcaatt cgccaatgtg
     9661 catccccacg gcggtgatca tcggcagcga ggcattgcgc agaatatggc ggcgttcggt
     9721 ctgtttgtca ttcaggccgc gtagccgcgc ccaggtgacg tgacgctgac cggcgacgtc
     9781 cagcatactg gcgcgcagca gtcgcgcgtt aatcgccagc gacataaagg cgatggaaac
     9841 cgcaggcaaa atgatgtgct gccagccgcc gtagcccatc gcgggtagcc attgcagata
     9901 caccgaaaac gccatcacta gcaaaaatgc cagccagaag ttgggcatcg acacgccaag
     9961 aaacgcgata aagcgcacgg cgaaatccgg caaacgatcg cggtggcgcg ccgcccagat
    10021 accgagcggt acggaagtga gcagaattaa taccagcgcc gcacctgcaa gttccagcgt
    10081 ggcgggcagg aagttcagca tatcgtccag caccgggcgt tggctggcga atgagatacc
    10141 aaagtcaaga tgcagcgcct tccacaacca ggtgccgtac tggacgtaca gcggctgatc
    10201 cagtccgagc atggtgcggg tagaggccag catctccggc gtcggcggca ggttagacag
    10261 acgcaaataa tcgagcgccg ggtcgccggt accgaggcgc agcatcagaa aaatgatcac
    10321 cgaggcggcg agcaccatcg ggatcagcag cagaaagcgg cgtaatacgt aacgcaacat
    10381 taaggtttca ccggtttaat ctgttcgaac ggaatttcgg tggcgatcgg cgcgtagggg
    10441 atgttaccca gctccggttt tgataccacc atcattgaga tgtaactgat aggcagataa
    10501 accgcctcgt catgcagacg ggtcagaatg tcgcgataca gcgcctgacg ttgcgtttcg
    10561 tcatgggtcg ccagcacttc gccgatctct ttatcaatca gcggtttgtc ggctaatcct
    10621 tgctgtgcct ggaagtcagc gtgcgacggt acgcgcattg aactcaggaa ggcgtgtgga
    10681 tcgtatggcg cgccccaggt gcggtggaaa atcatgccaa aacgaccgtc gcgctgacga
    10741 gcatagatac tgctctcttc ttcgccaatc agcgagacat ctgcgccaat ctggcgcata
    10801 tcagcctgaa tgatttccgc catcgatttg cttaacgcat cggtgccgat gaacgacagt
    10861 tcaatgcgca gcggctgacc atttttctcg cggatgtctt tgcccgcagg cagcgtccaa
    10921 ccggcttttt ccagcaacgc tttcgctttc tgcggatcgt actggcgcgg tttcaggccg
    10981 aggttggcgt agggcacaga aggggcaaac agggtgtcgg cgacctgctg ggtgccatac
    11041 aacgcgttat caatcagcga ttttttgttt accgcgtaat taagagcttc acgtactgcc
    11101 agctcattgg tgggggtttt ggcggtatta agcgccagca tcacggtttc gatcggctgt
    11161 gacagttggg tatggtaagc cgggttctgg ctaaagcggg cgaaggtatc gagcggtaat
    11221 aacccttcgt ttccgtacag caggtcgata tcgccagttt caaacgccac cgcgcgggta
    11281 gtcgggtccg ggatgacgtt aaaggtgatc tttttaatcg ctggcttttc gccccagtag
    11341 ttttcgttac ggacgaagac atcgtactga ttcagtttcg attcctgcaa aatccacggt
    11401 ccggtgccaa tcggcgcttt aattccgttc atggtttcat ggtttttaaa ctgcgaggga
    11461 gcgataaaac ggaaaggacg gggcagggcc agttcttgca ggaaaggata gtaggcgctt
    11521 ttcagggtaa tttgcagctc tgttttactg agtgctttaa catcaacaat ctggtttgcc
    11581 agctccagcc aggcgtgacg ttgacggtta tcgagcactg cgcggaagtt ttctgccgcc
    11641 gcctcggcat cgaacggttc accgttggat aatttcacgt catcacgcag ggtgaaggtc
    11701 caggttttac catcttctga atgagtccag ctttttgcca gccacgggat caccgaaccg
    11761 tctgcctgat atttcaccaa tggttcataa accatgctct gggcgaacat ctggttaggc
    11821 gtgtaaaggt gcgggtttag tggcccgaca ttcaccggcc aggcggtagt gatttcatct
    11881 ggtgcagcgg catggacgat aaaagacgca caagccagca gcgcaaatag agtgcggcgg
    11941 agtgtggaga gcatgataac cccaatggat taaaatagat ggcggaaata agtatgacga
    12001 ttttaagtat tcgtcatact gattacctgt tctggatcaa tagatgggca gtcgggtggg
    12061 cggatcagtt aactgaatcg atccattgca cactgtcggc ggtgagcgta aagggggtgg
    12121 gggtgcagat cgcaaggctt aaattttcta gctgacaatg gctgactgac agcgaggagt
    12181 gataggtgct atctacgctg acgatttgcc aggcgctgcc gccacgctgt ttaacgatgg
    12241 cttctttgcg cgtccagatg cgccagaaca tttccagttg ctgatcagga tgcacggcgt
    12301 ccatttcagc gtgttccccg aggctgaaca cggcgttcgc cagccagcgc cagttggcgc
    12361 gcgggcgaat cacttcgata tcgcagccga cttcgccttc atcgctcaac agcagagcga
    12421 tatcgtcacc gctatggctt aagttgaacc atagcggcgt ttccggcgcg aatgcaggtt
    12481 tgccttgttc gccatagatg atctccggta gcggggaaag cgtgtgcgaa agcaatgcac
    12541 gccccgccag ccagcgttcg cgtcgtggac cttgcggtgc ttgctcgcgt aaacccggtg
    12601 gcagtggagc tgcgcttaag gtcgaaactt tccccagaac tatccgatac atatcagggc
    12661 caacgtttaa tggaaaatga aagtgcgtat cgtatcactt gtcgcctcat cccggtaacc
    12721 gacttttcgg tctgcctggc cccagtaaaa tcgccagttt gctaccgcct ttggtggttt
    12781 ccatccagat tttacacacg ctggttaatg gcaccgaaag tagcatccct accgggccga
    12841 gcagccatcc ccaaattaac aatgaaagaa ataccaccat ggtggacatc cccaggcgat
    12901 ggcccatcat ccgtggttct aaaatattgc cgatgaccat atggaccact aaaaacaatg
    12961 cgccgaccag aatacatgcg taaacaccat taaacagcag cacctgaatc attggcggta
    13021 cggcggaaat taccgcgccg atattgggca cgtagttgag caaaaacgcc agtactgccc
    13081 acatcagcgc aaactgtacg cccattagcg ccagccccag ccagacgatg acacctgtcc
    13141 ataaactgag tagcgtcttt aacgccagat aatgcgaaac gcctttaagt gcgcggtgta
    13201 atcctgcgat gtgaatctgt gggttattca gcgcaaaacg cattttgtaa gggacgtggc
    13261 gcacttcaaa cagcataaaa actacggtca tcaccagcaa aagcacgctc gccattgccc
    13321 cggaaagccc gatcattagc gctgtggtga aggtaaccac tttttccgag tccatccgct
    13381 gcagcattcg ctccggcgac atatgcaaat taagaaaagg caacatctcc tgcaatttaa
    13441 aaagtttgcg cgtcagctcc ttattaaact tcggcagcat agagataaat tcgttaaacg
    13501 atgccgccag tacgccgact agcgcggtta gtgcgatcag catcaccacc actacaatcg
    13561 taatggtaac ggggcgttgt actccccgac gaataaacca ggtaacgagc gggttgagga
    13621 cgatggcaaa aaacagcgcc agtaacagct gcacaatgat atctgccgct gcgtgaatgc
    13681 ccgcgaggat cactaccagc gaggccaact tgagcagaat gtgcatgccc gttttatcgg
    13741 gttgaggggt ttccattggg gcttccttgt gactttttgt gttaagtgta gcgggagtca
    13801 cgtcagcaat attctgattc ttactgctga ttttcacgtc agcacgtggt aaaaatgaaa
    13861 cactgttgta aaaatgtggt gatcctcatg cccgaacccg tagccgaacc cgcgctaaac
    13921 ggattgcgcc tgaatttgcg cattgtctcc attgtcatgt ttaacttcgc cagctacctc
    13981 accatcgggt tgccgctcgc tgtattaccg ggctatgtcc atgatgtgat gggctttagc
    14041 gctttctggg caggattggt tatcagcctg caatatttcg ccaccttgct gagccgtcct
    14101 catgccggac gttacgccga tttgctgggc cccaaaaaga ttgtcgtctt cggtttatgc
    14161 ggctgctttt tgagcggtct gggatatctg acggcaggat taaccgccag tctgcccgtc
    14221 atcagcctgt tattactttg cctgggacgc gtgatccttg ggattgggca aagttttgcc
    14281 ggaacgggat cgaccctgtg gggcgttggc gtggttggct cgctgcatat cgggcgagtg
    14341 atttcgtgga acggcattgt cacttacggg gcgatggcga tgggtgcgcc gttaggcgtc
    14401 gtgttttatc actggggcgg cttgcaggcg ttagcgttaa tcattatggg cgtggcgctg
    14461 gtggccattt tgttggcgat cccgcgtccg acggtaaaag ccagtaaagg caaaccgctg
    14521 ccgtttcgcg cggtgcttgg gcgcgtctgg ctgtacggta tggcactggc actggcttct
    14581 gccggatttg gcgtcatcgc cacctttatc acgctgtttt atgacgctaa aagttgggac
    14641 ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg tcggtacgcg tttgttattc
    14701 cctaacggca ttaaccgtat cggcggctta aacgtagcga tgatttgctt tagcgttgag
    14761 ataatcggcc tgctactggt tggcgtggcg actatgccgt ggatggcgaa aatcggcgtc
    14821 ttactggcgg gggcagggtt ttcgctggtg ttcccggcat taggtgtagt ggcggtaaaa
    14881 gcggttccgc agcaaaatca gggggcggcg ctggcaactt acaccgtatt tatggattta
    14941 tcgcttggcg tgaccggacc actggctggg ctggtgatga gctgggcggg cgtaccggtg
    15001 atttatctgg cggcggcggg actggtcgca atcgcgttat tactgacgtg gcgattaaaa
    15061 aaacggcctc cggaacacgt ccctgaggcc gcctcatcat cttaaaactt actgaataac
    15121 cagcgtatta atgatgtttt ctgcggtggt ctgcgctttt tgctgatcgt cagcgggcag
    15181 cgtaatttgc atggtcagca gttgattacc cacgttaccc agaataacgg aagagtacgc
    15241 cgtctggcct ttcgcggaga taatactgtc taactgctgc attttgtgac ctttcagctc
    15301 aatggcttta ttggttacca cttgcagctg cggatcgcgg ctacgttgct gatcttccag
    15361 acgcttcgcc agcaccgcca gatcttcttt cggatcatcg cccatgatga cgatgactgc
    15421 tttctgcccg gtggcgtcgg accagacatg catgttattg gcctgcgttc ccagcttacc
    15481 gctctggtcg gtcatatccg ctggcagcga gaaacttaac ttgccatcaa gcaggttgac
    15541 gggattcccg gtagcgttat tttccgcgac cgaaccctgc gccgtagcgt tagtgtcttt
    15601 atcatcacag gccgcaagcc ccataaccag caggccaatt ccgacatatt taaccagatt
    15661 gcgcattgac ttcttccttt cgataaacgg ccataacggc tcattcatcc atcttatcac
    15721 aactctgata acgaaccttt aactcgcctg caaagcgttg atttcggatt tatctgccag
    15781 tcttttcaac agcatattga gtaatacgcc atacattggc aggaagaaaa cgatactgat
    15841 taacactttg aaacagtaat cgaccagcgc gatttccatc cagtgttcag ccataaaggc
    15901 atccgggcta cgccagaagg caatgaagaa aaaggccagc gtgtcgctga cgttaccgaa
    15961 cagtgtggac gctgtcggtg ccagccacca gcggcgactc tgacgcaggc ggttaaaaac
    16021 gtgcacgtcg aggatctgtc ccagcgcgta ggccatgaaa ctggcggtgg cgatacgggc
    16081 gacaaacagg ttgaagtggg cgagtgcgcc gaatccctgc caggaaccca tatagaatag
    16141 cgacgagatg acgtaggaga tcagcaacgc cgggatcatc accgcaaaga tgatacgacg
    16201 ggccagcggt gcgccaaaaa tacgcacggt caggtcggta gcaaggaaaa taaacgggaa
    16261 gctaaacgcg ccccaggtgg tatggaaacc caaaatggag acgggaagct gaaccagata
    16321 gttactggag gtgatcacca gcagatgaaa taacgataac cagaacaacg ccttatagcg
    16381 ttgagtttgc gagaaaacgt tcatattgta cctttttgat taaccattgg ggtgagggaa
    16441 cccaatacgt acgacacgtc tgttacctta acataataac gtgcttaccc ttttttcgag
    16501 ccgccgcatg atactgcttt acgttgacaa tgcaatggtt aattttcacg caatcgttaa
    16561 cctggtttgc ttacggactc acagggcgta aactagcgcc gtttttttat gtgatgagaa
    16621 gaaaatgacc gatctctttt ccagccctga ccacacactc gacgcacttg gcctgcgctg
    16681 cccggaaccc gtaatgatgg tgcgcaaaac tgtgcgcaat atgcagcccg gcgaaacgtt
    16741 gctgattatc gccgacgatc cggccactac ccgcgatatt cctgggtttt gtacctttat
    16801 ggaacacgaa ctggttgcga aagagacgga tggactgcct tatcgttatt tgattcgtaa
    16861 aggcggttga tgggggctga ttggcttcga tgccgccttt tcccctcacc ctaaccctct
    16921 ccccagaggg gcgaggggac cgatcgcgct caatgttgcg atcggtttgc cttatctcct
    16981 gcgcaacaat cttaacgcat tcgctgtcac cagcaccgtc gcacccgtat ctgccagcac
    17041 cgccagccat agtccggtca tccctaaaag cgtggtgacg aggaagatcc ctttcagtcc
    17101 cagcgcaatg gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca gttcaatcat
    17161 ttgcaccagg ccgcgcaggt ggttatgggt taatgctgca tcagcggttt ccagcgccac
    17221 gtctgtgcca ctgcccattg caatcccaat ggcggcagct ttcatcgctg gcgcgtcgtt
    17281 aataccgtca ccgaccatcg ccagcggcgc atgttgattc agctcggtca ccgctttgac
    17341 tttatattcc ggcaacaggc ccgctttaaa ttccagcccc agctccccgg caattgccgc
    17401 cgctgcgcgt ggattatcgc cggtgaggat cacccctttg acgcccagcg cgttcagttc
    17461 actgatggca gttgcagcat cggcgcgcag ggtatcctgc aatgcaatga tacccagcac
    17521 gtcatcatta cgtactacca gcacaaccgt ttgcccggcg ctttccagtt cattaatcaa
    17581 accagcaaat gcatcagcgg gatgtttccc ggcagcgcat atcaacacgc gctcaccgtt
    17641 aacctgcgct tcaatgccag acccgaccag cgcccgctgt gattcggcgg cgggaatggc
    17701 gagtgcagca acctgtgctt cgcgcacgat ggcctgcgcc agtggatgcg tcgcgctttg
    17761 ctcgaccgcc gccgccagtg tcagcagttc agattcacta atacccgttg ccggatgaat
    17821 cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt ttatcaaacg ccacctgagt
    17881 aacacgacct agttgttcca gcgccgctcc gcctttaatc aacgccccac gacgcgctgc
    17941 tgccgccagc ccggaggtaa tcgccgcggg cgtggagata actaacgcac acgggcagcc
    18001 aatcagcagc agcgtcaggc ctttataaat ccactcctgc cagctggcgg caaacagcag
    18061 cggtggcacc agcgtcacca gcagggcgac ggccataatc gctggcgtat aaatacggct
    18121 gaaacggtcg ataaaccgct caatgggggc gcggcgctct tcggcttctt cgatcagttt
    18181 cagaatccgg tcaatggcgc tggcacccgg ttctgacagc acttccagcg ttaccagacg
    18241 gtctacgctg gtggctcctg caggaacttt atcgcccgtc gcgcgctcca ccggaatgga
    18301 ttctccggtc agggcgcttt catcaaaact ggcaaacggt gagagcagtt taccgtcggc
    18361 aggcaaacgc ccacctgcgg cgacttcaat cacatcgcca ggccgcaggc tgttaatcgc
    18421 cacctcttcc cgctcaccgt tacgcaggcg cgtggcggtt tctggtttca gcgccattaa
    18481 cgcgctaacg ccctgacgcg cgcggctggc ggcccagcct tccagtcgtt caccaatcaa
    18541 aaacagcagc aacaccatcg cagcttcagc cgttgcgcca ataaacagtg caccaatagc
    18601 ggctacgctc attaaggttt caatggcgaa gtagctgccg gatttgatca gccgtaatgc
    18661 ctgatgagca atcgggtaca gcccaaccag cgtggtcgcg ataaacgcca gttgcccgaa
    18721 cggatgattg aactgctcca gaccccagct gattgccatc atcacgatta gtgtaatcag
    18781 cggcagattc tctttcaggc gcgatgcttg tggctcgtcg gcggcctgct catcgcgcag
    18841 ggaatagccc gctttttgca ccgcagattc aacttgtgca cgaatgtcat tgtcggcatc
    18901 aaccaccagt ttttcggtgg cgaacaacac ctgcacctga ttcacacctg caagctggcg
    18961 cacggcattt tctaccttgc gcgcacaggc ggcgcagtcc atgccactga ctttccagct
    19021 atagcgggtg ccggagacgt tttcagagag cgttggcgag ctggagcatg cgccgtcgca
    19081 gcaacagtcg ttggcgttct gtaccgtggt tagcggtttg aacgcagcaa attgaggggc
    19141 tttcttgccg tgattgtcag gtgtcgacat ggcatactcc ggttaagttt tttctcatta
    19201 accgaaggat acactctgga gtcgactcca gagtcaagtt ttatcagaaa tacagtgagc
    19261 ggacgatgag gaagtgaccg gcaaagtagc aggccgcagc aatcgcgtta tccgcacgga
    19321 agcgtcggcg atagtggctc cccagccaga caaagttact gataaacagc aacgaagcgc
    19381 caacaaacgc agagagcgcc ggagcggtcg gacggaagaa ccacagttca cctgccagcc
    19441 acaccatcac cagcgtcatg ccgataaacg tgcagatagg ccaacgatac tcttccagcc
    19501 gcgtccagat aatcgccagt aacagcgcac ccagaaccag caacaccagt ggcagaggcc
    19561 agaagaaaga gagcgtcatc tgactggcga aatagatggt gtacagcagg tgcgagagga
    19621 aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa cagggttagc gcatcgccca
    19681 gcagtgaggc gcacagccct gccagcacca gatagctaat ggcgtcgaac atcggcgctt
    19741 gccaggccag taacagcagg agaagaaggg ttaacggttt aaacacccag cgttgccagg
    19801 ttggcccacg atacgatgca tccacagata gccatgcgga aagacagaca gcgataaacg
    19861 accaaagcat cttagttcct tgaattcgtt attttttcgt aagcggaatg cctacggtct
    19921 tgcttccagt ttagtggcga gggtggacgg atgacaacac cataactagc agggtatgct
    19981 tatttccgca ttttccgatg agggataaag atgagcaaac cacctctttt ctttattgtt
    20041 atcattggct taattgtcgt cgccgcatcg tttcgtttta tgcaacagcg acgggaaaaa
    20101 gctgataatg atatggctcc gctccagcaa aagctggtgg tggtgagcaa caagcgggaa
    20161 aaaccgatta acgatcgccg ttcgcgccag caggaagtga ctccggcagg caccagtatg
    20221 cgctatgagg caagcttcaa accgcaaagc ggaggaatgg agcagacgtt tcgcctcgac
    20281 gcccagcagt accatgccct gacagtgggc gataaaggta cgctgagcta taaaggaacg
    20341 cgctttgtta gctttgttgg cgaacaataa ggttattttt taactttaaa tttcttctgc
    20401 cagaccagca gttcaaacac gccgaaaagg aaaatccgca ccttttcggc ggtggtcatc
    20461 tgcgggccat ctttcggtaa agtggatttc aatagcgcca gctgcatacc atgcatcagc
    20521 acggtaaaaa tcagcgccac gttaacgaaa atattcagcg ggcgcgggaa gggatgcacc
    20581 agattgagga ttaaaaatcc ccaaacgcag agcattaaca aacgaccaat attaatcagc
    20641 atcactttct ccttgtgctt cgcgttgata cagccgataa gccacctgac ccgccacttt
    20701 ttcccgatgt aatgaccagt ttgccggaac agtgggcaga ccgttttcga cttcgctttc
    20761 gacataaatc agggcttcgt cagccagcca gccgttatct tccagtaaat ttatcgtctc
    20821 ttctaacaag ccacggcgga acggtggatc gacaaacacg atattatgcg gtgtaccttt
    20881 ttgcgccagg aatgacatcg cgttgctgtt caccacgcgt gcattgcctg cttttagtgt
    20941 cgccagattc ttaattaact gctgagaaac cgcgcgatcc atctcaatca acgtggcccc
    21001 cgcagcgtag cgcgataacg cttccagtcc cagcgcgccg ctcccggcga agcaatccag
    21061 acattgggcg tcaacaatga ccggagccag ccagttaaac aatgtttcgc gtacgcggtc
    21121 ggtggtgggg cgcagacctg ggctatcagg aaccgggagt ttacggcctc gccactgccc
    21181 gccaataatg cggatttggc cgctgccgga atgattcggt tttttcatga taaattgctc
    21241 aatccgccag ataacacata cttgcaggcg gtgatgtgaa ttaagttaag tgatagacta
    21301 tttcatcatt tttttagctg ctatgtacat agcgttaacg ctgtgccatg aagcaacagc
    21361 gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg ctttttttcc tggctgggct
    21421 ttggtcaaaa agagcagacc ccggaaaaag agacagaagt tcagaatgaa caaccggttg
    21481 tagaagaaat cgttcaggcg caagagcctg cgaaggcttc tgaacaagcc gtcgaagagc
    21541 aaccgcaggc gcatactgaa gccgaggcgg aaacttttgc tgccgacgtt gtggaagtca
    21601 ctgaacaggt tgctgaaagt gaaaaagcgc agcctgaagc ggaagtcgtt gcacagccgg
    21661 aaccggtcgt agaagaaacg ccggagccag tggctatcga acgtgaagag ctgccgttgc
    21721 cggaagacgt caacgccgaa gaggtttcgc cagaagagtg gcaggctgaa gcggaaaccg
    21781 tagagattgt cgaagcggcg gaagaagaag cggctaaaga agaaatcacc gacgaagagc
    21841 tggaagcaca ggcgctggct gccgaagcgg cagaagaggc ggtgatggtg gttcctccgg
    21901 tagaagagca gccggtggaa gaaatcgctc aggagcagga aaaaccgacc aaagaaggtt
    21961 tcttcgcgcg cctgaaacgc agcctgttaa aaaccaaaga aaatctcggt tccggattta
    22021 tcagcctgtt ccgcggtaaa aaaatcgacg atgatctgtt tgaggagctg gaagaacagc
    22081 tgttgatcgc cgatgtgggc gtggaaacca cacgtaaaat tatcaccaat ctgacggaag
    22141 gcgcatcccg caagcagctt cgtgatgccg aggcgctcta tggcctgctg aaagaagaga
    22201 tgggcgagat tctggcgaaa gtcgatgagc cgctgaatgt tgaaggtaaa acgccgttcg
    22261 tgatcctgat ggtgggcgtc aacggtgtgg gtaaaactac gacgattggt aagctggcac
    22321 gtcagtttga gctgcagggt aaatcggtga tgctggcggc gggcgatact ttccgtgcag
    22381 cagcggttga acagcttcag gtctggggtc agcgcaacaa tattccggtg attgcccagc
    22441 ataccggtgc ggattccgcc tctgttatct tcgacgccat tcaggcagct aaagcgcgta
    22501 atatcgacgt cctgattgcc gataccgccg gacgcctgca gaacaaatcg cacctgatgg
    22561 aagagttgaa gaaaatcgtc cgcgtgatga agaaactcga cgttgaagcg ccgcatgaag
    22621 ttatgctgac tattgatgcc agtaccgggc agaatgcggt aagccaggcc aaactgttcc
    22681 acgaagccgt tggcttaacc ggcatcacgc taacgaaact ggacggcacg gcgaaaggcg
    22741 gggtaatttt ctcggtggct gaccagtttg gtatccctat ccgctacatt ggtgtcggcg
    22801 aacgtattga ggatttgcgt ccgtttaagg cggacgactt tatagaggca ctttttgccc
    22861 gagaggatta acaatgattc gctttgaaca tgtcagcaag gcttatctcg gtgggagaca
    22921 ggcgctgcag ggcgttacgt tccatatgca gccgggtgag atggcgtttc tgaccggtca
    22981 ttccggcgca gggaaaagta ccctcctgaa gctgatctgt gggattgagc ggcccagcgc
    23041 cgggaaaatc tggtttagcg gccatgacat cacgcgtctg aaaaaccgtg aagttccgtt
    23101 tctgcgccgc cagattggca tgattttcca ggatcaccat ctactgatgg accgtactgt
    23161 ctacgataac gtggcgatcc cgctgattat cgctggtgcc agcggtgacg atattcgtcg
    23221 ccgggtgtcg gcggcactgg ataaagtcgg gctactggac aaagcgaaga acttccctat
    23281 tcagctttcc ggcggtgaac aacagcgtgt tggcattgcc cgcgcggtgg tgaataagcc
    23341 cgcggtactg ctggcggacg aaccgaccgg taacctggac gacgcgttgt cggaagggat
    23401 tctacgtctg tttgaagagt ttaaccgcgt tggggtaacc gtattgatgg caacgcacga
    23461 catcaacctg atttcacggc gttcctatcg catgctcacc ctgagcgatg gtcacttgca
    23521 tggaggcgtg ggccatgaat aagcgcgatg caatcaatca tattcggcag tttggtgggc
    23581 gtctggatcg cttccgtaaa tcggtcggcg gctcaggcga cggcggtcgt aacgcaccga
    23641 aacgcgcgaa atcctcgcca aaaccggtaa atcgcaaaac caacgttttc aacgaacagg
    23701 tgcgctatgc cttccacggc gcattgcagg atctgaaaag caaaccgttc gccacgtttt
    23761 taacggtgat ggttatcgcc atctccctga cgctgcccag cgtctgttat atggtgtaca
    23821 aaaacgttaa tcaggcggcg acgcagtatt atccgtcacc gcaaatcact gtttacttgc
    23881 aaaaaacgct ggacgatgac gccgctgcgg gcgtggtggc acagttgcag gccgagcaag
    23941 gcgtggagaa agtgaactat ctttctcgtg aagacgcact gggcgagttc cgtaactggt
    24001 ctggttttgg tggtgcgctg gatatgctgg aagaaaaccc gcttccggca gtggcggtgg
    24061 tgatcccgaa actcgatttt caggggacgg aatcactcaa tacgctgcgt gatcgtatca
    24121 cgcagattaa cggcattgac gaagtgcgga tggatgacag ctggtttgcc cgtctggcgg
    24181 cgttgaccgg gctggtcggg cgcgtttcgg cgatgatcgg cgtgttgatg gtggcggcag
    24241 tgttcctcgt catcggcaac agcgtacgtc tgagcatctt tgctcgccgt gactccatca
    24301 acgtgcagaa actgattggt gcgacggatg gtttcatcct tcgcccgttc ctgtatggtg
    24361 gcgcactgct gggattttct ggcgcattgt tgtcattaat tttgtcagaa attctggtgc
    24421 tgcgattgtc atcggcggtt gcggaagtgg cacaggtttt cggaacgaat tttgatatca
    24481 atggtttatc gttcgatgaa tgcctgctat tgctgctggt atgctcgatg attggctggg
    24541 tggcagcgtg gcttgccacg gtacaacatt tacgccactt tacgcctgaa taataaaagc
    24601 gtgttatact ctttccctgc aatgggttcc gtagcaggga aagagatccc gttgtctctt
    24661 cccggtattt catctctatg tcacattttg tgcgtaattt attcacaagc ttgcattgaa
    24721 cttgtggata aaatcacggt ctgataaaac agtgaatgat aacctcgttg ctcttaagct
    24781 ctggcacagt tgttgctacc actgaagcgc cagaagatat cgattgagag gatttgaatg
    24841 actgacaaaa tgcaaagttt agctttagcc ccagttggca acctggattc ctacatccgg
    24901 gcagctaacg cgtggccgat gttgtcggct gacgaggagc gggcgctggc tgaaaagctg
    24961 cattaccatg gcgatctgga agcagctaaa acgctgatcc tgtctcacct gcggtttgtt
    25021 gttcatattg ctcgtaatta tgcgggctat ggcctgccac aggcggattt gattcaggaa
    25081 ggtaacatcg gcctgatgaa agctgtgcgc cgtttcaacc cggaagtggg tgtgcgcctg
    25141 gtctccttcg ccgttcactg gatcaaagca gagatccacg aatacgttct gcgtaactgg
    25201 cgtatcgtca aagttgcgac caccaaagcg cagcgcaaac tgttcttcaa cctgcgtaaa
    25261 actaagcagc gtctgggctg gtttaaccag gatgaagtcg aaatggtggc ccgtgaactg
    25321 ggcgtaacca gcaaagacgt tcgtgagatg gaatcacgta tggcggcaca ggacatgacc
    25381 tttgatctgt cttccgacga cgattccgac agccagccga tggcaccggt gctctatctg
    25441 caggataaat catctaactt tgccgacggc atcgaagatg ataactggga agagcaggcg
    25501 gcaaaccgtc tgaccgacgc gatgcaaggt ctggacgaac gcagccagga catcatccgc
    25561 gcgcgctggc tggacgaaga caacaagtcc acgttgcagg aactggctga ccgttacggt
    25621 gtttccgctg aacgtgtacg ccagctggaa aagaacgcga tgaaaaaact gcgcgctgct
    25681 atagaagcgt aatttccgct attaagcaga gaaccctgga tgagagtccg gggtttttgt
    25741 tttttgggcc tctacaataa tcaattcccc ctccggcaaa acgtcaatcc ccacgcagat
    25801 tgttaataaa ctgtcaaaat agctattcca atatcataaa aatggggtat gttttagcag
    25861 agtatgctgc taaagcacgg gtagtcatgc ataaaacgaa ataaagtgct aaatcacaac
    25921 accacaacac acgtaataac cagaagaatg gggattctca ggatgaatat aaagggtaaa
    25981 gcgttactgg caggatgtat cgcgctggca ttcagcaata tggctctggc agaagatatt
    26041 aaagtcgcgg tcgtgggcgc aatgtccggt ccggttgcgc agtacggtga ccaggagttt
    26101 accggcgcag agcaggcggt tgcggatatc aacgctaaag gcggcattaa aggcaacaaa
    26161 ctgcaaatcg taaaatatga cgatgcctgt gacccgaaac aggcggttgc ggtggcgaac
    26221 aaagtcgtta acgacggcat taaatatgtg attggtcacc tctgttcttc atcaacgcag
    26281 cctgcgtctg atatctacga agacgaaggc attttaatga tcaccccagc ggcaaccgcg
    26341 ccggagctga ccgcccgtgg ctatcagctg atcctgcgca ccaccggcct ggactccgac
    26401 caggggccga cggcggcgaa atatattctt gagaaagtga aaccgcagcg tattgctatc
    26461 gttcacgaca aacagcaata cggcgaaggt ctggcgcgag cggtgcagga cggcctgaag
    26521 aaaggcaatg ctaacgtggt gttctttgat ggtatcaccg ccggggaaaa agatttctca
    26581 acgctggtgg cgcgtctgaa aaaagagaat atcgacttcg tttactacgg cggttatcac
    26641 ccggaaatgg ggcaaatcct gcgtcaggca cgcgcggcag ggctgaaaac tcagtttatg
    26701 gggccggaag gtgtggctaa cgtttcgctg tctaacattg cgggcgaatc agcggaaggg
    26761 ctgctggtga ccaagccgaa gaactacgat caggttccgg cgaacaaacc cattgttgac
    26821 gccatcaaag cgaaaaaaca ggacccaagc ggcgcattcg tttggaccac ctacgccgcg
    26881 ctgcaatctt tgcaggcggg cctgaatcag tctgacgatc cggctgagat cgccaaatac
    26941 ctgaaagcga actccgtgga taccgtgatg ggtccgctga cgtgggatga gaaaggtgat
    27001 ctgaaaggct ttgagttcgg cgtatttgac tggcacgcca acggcacggc gaccgatgcg
    27061 aagtaatcat taatcggcaa ctttgggttg ccgccaaatt gctaatatcg agtacgttgc
    27121 ttcatgccgg atgcggcgta aacgccttat ccggcctaca agatccaaag aaatcagtaa
    27181 attgcaacac acattgtagg cctgataagc gtagcgcatc aggcaatttc tcgttggtca
    27241 tcacttttga aatcaacttg acgattaacg cttctcccag ccgccctgtt gcgccgtaaa
    27301 tcccag
//
LOCUS       DAJTYD010000036        27196 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTYD010000036 DAJTYD010000000
VERSION     DAJTYD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.036"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(40..1338)
                     /gene="eno"
                     /locus_tag="OO585_001418"
     CDS             complement(40..1338)
                     /gene="eno"
                     /locus_tag="OO585_001418"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6629054.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OO585_001419"
     CDS             complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OO585_001419"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6629055.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OO585_001420"
     CDS             complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OO585_001420"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6629056.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEESYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OO585_001421"
     CDS             complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OO585_001421"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6629057.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OO585_001422"
     CDS             complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OO585_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6629058.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4814..7048)
                     /locus_tag="OO585_001423"
     CDS             complement(4814..7048)
                     /locus_tag="OO585_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6629059.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OO585_001424"
     CDS             complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OO585_001424"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6629060.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8454..11210
                     /gene="barA"
                     /locus_tag="OO585_001425"
     CDS             8454..11210
                     /gene="barA"
                     /locus_tag="OO585_001425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6629061.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OO585_001426"
     CDS             complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OO585_001426"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6629062.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12803..14143)
                     /locus_tag="OO585_001427"
     CDS             complement(12803..14143)
                     /locus_tag="OO585_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6629063.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OO585_001428"
     CDS             complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OO585_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6629064.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15932..16381)
                     /locus_tag="OO585_001429"
     CDS             complement(15932..16381)
                     /locus_tag="OO585_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6629065.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OO585_001430"
     CDS             complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OO585_001430"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6629066.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17181..17510)
                     /locus_tag="OO585_001431"
     CDS             complement(17181..17510)
                     /locus_tag="OO585_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6629067.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OO585_001432"
     CDS             complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OO585_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6629068.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18745..19593
                     /gene="queF"
                     /locus_tag="OO585_001433"
     CDS             18745..19593
                     /gene="queF"
                     /locus_tag="OO585_001433"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6629069.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPKVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19705..21069
                     /gene="ppnN"
                     /locus_tag="OO585_001434"
                     /gene_synonym="ygdH"
     CDS             19705..21069
                     /gene="ppnN"
                     /locus_tag="OO585_001434"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6629070.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKAVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21626..22915
                     /gene="sdaC"
                     /locus_tag="OO585_001435"
     CDS             21626..22915
                     /gene="sdaC"
                     /locus_tag="OO585_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6629071.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22973..24340
                     /gene="sdaB"
                     /locus_tag="OO585_001436"
     CDS             22973..24340
                     /gene="sdaB"
                     /locus_tag="OO585_001436"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6629072.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24452..25207
                     /gene="xni"
                     /locus_tag="OO585_001437"
     CDS             24452..25207
                     /gene="xni"
                     /locus_tag="OO585_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6629073.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OO585_001438"
                     /pseudo
     CDS             complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OO585_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OO585_001439"
     CDS             complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OO585_001439"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6629074.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6575 a         7293 c         6554 g         6774 t
ORIGIN      
        1 tcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc ctttgatctc
       61 tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa tcagctggtt
      121 gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc ctgcagcagt
      181 accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt gagagataac
      241 tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg tcagagaacc
      301 gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac cttctttcag
      361 gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt cgcccagaac
      421 tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt cgatagaaac
      481 gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag aggtgaacgc
      541 tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag aagctgcgca
      601 gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa cagcttcagc
      661 gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac cttcgtcacc
      721 aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga aaacttcaga
      781 acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca tgaattcctg
      841 gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca tcggaaccgg
      901 catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca gcggcatacc
      961 tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg cgttcgcgcc
     1021 gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg
     1081 atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc
     1141 tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc
     1201 acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac caccctccag
     1261 atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac gaccgatgat
     1321 ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa actccagaca
     1381 aacaacacgt acccagggta cgcgttgccg ttctaacttt tttacttact tcgcctgacg
     1441 tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg
     1501 agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat tcggaacttc
     1561 gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac ctgcatcttc
     1621 aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct caacaattgt
     1681 cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact gctgtgcgcc
     1741 gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt tgccgttttc
     1801 atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca caaattccgt
     1861 agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat
     1921 acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat
     1981 catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt ccagaccttt
     2041 aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga tgttgacgct
     2101 gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat aagcatccgg
     2161 cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt tcgcttcttc
     2221 gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc tgaatcgttt
     2281 acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat agatggaatc
     2341 gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg caatcttcgc
     2401 acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag gctggatacc
     2461 gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt caccagacgc
     2521 tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc caatttcaac
     2581 agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta ctgtaccgcc
     2641 gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct ctttgattgc
     2701 gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt
     2761 acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc tcattttggt
     2821 acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt cttcagtaac
     2881 gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga tgtacggatc
     2941 cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga gggaggctgc
     3001 ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat agttcgttgt
     3061 catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac gggaaagcag
     3121 tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg ccagcgcact
     3181 attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac gcaaatcaag
     3241 cgcttgtcat ttaaaaaatg acacaaatgg cacttgaccg cgtaattccc ttagagatca
     3301 atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc aacacctgtc
     3361 atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt
     3421 tcgttcgctt tttgcaatgc gatttctgct ttcgtcccta aatggcgagc cagattaacc
     3481 gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac aacagcctgc
     3541 cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcaaccac cgggccaagc
     3601 gtcgtccagt cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc ccgcattaaa
     3661 gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg cgcgcgctct
     3721 tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc agaactatca
     3781 gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca aatatcatta
     3841 aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga
     3901 tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg
     3961 tagctttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt atcccacggg
     4021 cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacgatc gatttgattc
     4081 attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca gaatagaagt
     4141 gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt gtaattcctc
     4201 tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgattc tttttacctg
     4261 atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga acggatatcc
     4321 ttttgattgc gttgtacaag gaacacacag acacatacct gttttgttgt tgtacatgaa
     4381 aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg tcgggtcaaa
     4441 atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta ccagacttcc
     4501 ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt gatgtcgttg
     4561 accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat taatttgcca
     4621 tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg cattaacgta
     4681 gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg gatcatcacc
     4741 ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat tgtagatacg
     4801 agcaaatttc tgcctaactc ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt
     4861 tgagtttacc cagcacgcgg cccagcacct gcaggttgta aatctcaatg gtcatgtcga
     4921 tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagtacg ttcaccttct
     4981 cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta gccaccacgc
     5041 ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca acaatgcgtt
     5101 ctggcgcatg agagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac
     5161 cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg cagcagcgcg
     5221 cgatgtggtg catcaggttg ccaacacctt cgactaccac gcgaccgtta tctttactgc
     5281 ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg tcctgctctt
     5341 cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg agacggatat
     5401 ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa cgcggcagca
     5461 gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg tcaaggattt
     5521 gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg
     5581 aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca cggctggggt
     5641 tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg taggtgaacg
     5701 gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca ctgtggatgt
     5761 ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca cctttcggcg
     5821 taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc atttcgccgg
     5881 aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc cggtcttcat
     5941 gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc gcagcaacac
     6001 ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg gttttgccac
     6061 ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg ttagcgacat
     6121 aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc agtgcggcat
     6181 aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac agctcatcga
     6241 aggcgaggtt ctttttctgc attttgcgcc agatgctgta gatgtgtttc ggacggccat
     6301 acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatgaccg acaaactctt
     6361 cgatatagtg ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg cgtttgtatt
     6421 cggtcgggtg cagataacgg aagcagtaat cttccagctc ccatttcagt tgcccaatcc
     6481 caagacggtt cgccaacggc gcgtagatat tggtgcactc ttttgccgcc agtacgcgtt
     6541 catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc agtttgatga
     6601 ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta tcgacctgtt
     6661 cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca
     6721 cgccgtgaat aaggttaacg acagacttac cgacgctctc acgcagcaca tcttcgctga
     6781 ctacgttggc atcggccaga gggaacagca gcgccgcccg cagcgtgtca atgtccatac
     6841 ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga ctggcatccg
     6901 gatgcccctg cgtctgctgc agacaatacg cccaggtttc ggctaagcac tcacacgact
     6961 tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca ccagccttat
     7021 tgatatgtgc acttcttacc gcaaccattg tcctctcctt tagggaccag acctgccgaa
     7081 atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca gatgtcccgt
     7141 gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt ttaataacgc
     7201 ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac gaataggttc
     7261 cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt ccagcaacac
     7321 tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa gattttcgtg
     7381 ataaaacgtc acattctgta agccattaag acgcgcattc tgctggcctt tttccaccag
     7441 cgccggaaca ccttctacac cgaccacact ggcagcttgt gtcgccaatg gcagtgtaaa
     7501 gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat ccagccattc
     7561 caacgcactc gctaccattt tttggttcac acccgcattg acctgaataa aatcgcgcgg
     7621 gctaaaagtt aagcgcaacc cgttagagtc ataccagggc atctcaccag agacggtttc
     7681 gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg aaaagcgttc
     7741 cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc
     7801 gctggttgcc tgtaccagtt caacatgacc aagatggcgc attgcttgta agctgcccag
     7861 gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg gacattgttt
     7921 aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt
     7981 cggtaagtag ttcagactta aacgcgcgcg acggcgatag ccccagggaa catcggcgat
     8041 cacttcagag acttcgtgtt tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg
     8101 taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat gaggacagcg
     8161 tggcgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc gggcatactg
     8221 ttttttatct tcagtaacag taacttccgc gttttcctgc ggcaataatc cggggataaa
     8281 tagcgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga
     8341 aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg
     8401 cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac tccatgacca
     8461 actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc cttattggtt
     8521 tattgctgag tatatttttc gtcgtgcatc gctataacga cttgcagcgt caactggaag
     8581 atgcaggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc atgagcctgc
     8641 aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat tccgatattg
     8701 ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc aattttcatc
     8761 ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag ctcactgtca
     8821 ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt tactcccccg
     8881 acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat attgcgcttg
     8941 agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt tccagcgtga
     9001 tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg cgcgatgtaa
     9061 ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca
     9121 gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt atcaactcga
     9181 tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac caggcgacgt
     9241 ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagttg gatctggcga
     9301 aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat atgtcacacg
     9361 agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg aaaacagaat
     9421 taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat aatttgctgg
     9481 caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg attctggaaa
     9541 gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg gcacattctt
     9601 ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct gataacgtga
     9661 tcggcgaccc actgcgatta cagcaaatca tcactaacct ggtggggaat gcaattaaat
     9721 tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt aataccaaag
     9781 tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc gatcaatcgc
     9841 gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat ggtggcaccg
     9901 ggctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat atttcgttcc
     9961 atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat ctgaacccga
    10021 acattattat cgaagggcca tccacccagt gcctcgcagg taagcgcctg gcctatgtcg
    10081 aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg ccgctggaag
    10141 tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg atgttgttag
    10201 gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg
    10261 tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc aatgctgaaa
    10321 aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct acacgcctgt
    10381 tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg
    10441 aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac ctgaaactta
    10501 tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc gggcatcagg
    10561 cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt caaatgccgg
    10621 acatggatgg cattcgggcc tgcgaactca tccaccagct cccccatcag caacaaacgc
    10681 cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg cttggcgcag
    10741 ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat ttgttgttgc
    10801 gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc aacgaaattg
    10861 tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca gcaggaaaaa
    10921 ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca
    10981 aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg attcataaac
    11041 tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg
    11101 aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg ctggaactgt
    11161 tggacgagat ggataatgtc gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt
    11221 gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt
    11281 ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa attcaatgaa
    11341 ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt tagcatcagt
    11401 ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac catgcacggg
    11461 cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc attgtcacgc
    11521 gcgcaaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc catatcgatt
    11581 tctacaccca gccccggttt ttccggcacc tgaaccagac cgcctttgat ctcaaacggt
    11641 tctttggtca agcgctgatt gccttcctgc caaatccagt gcgtatcaat cgcggtgatt
    11701 ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg
    11761 tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac acgtaccgaa
    11821 ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg cagggagagc
    11881 gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg caggccagtt
    11941 gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga
    12001 tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc ttcgttcagc
    12061 gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc
    12121 acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg
    12181 aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct
    12241 tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcagc
    12301 ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac ttcgctacgc
    12361 tgttgaccat cgcccagcag ggatgccacg tttaccccca gatgctgccc cagcagatcc
    12421 agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc
    12481 aaaccgcgcc caccagcatc acgatcggca aaagtattac gcaccagcgt cagaacgttt
    12541 ttgtattcac ccagcgtttt acctaccacc agcggaatcg catcttccag cgttttacgg
    12601 attttctcgc cgccgggaat ttcccctacg ccagtgtgac cagaattatc tttgataatc
    12661 ataatattac gcgtaaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca
    12721 tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc
    12781 atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta cggtcaaagg
    12841 tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg ccaggcagac
    12901 gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg
    12961 gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg gtcaggcgac
    13021 aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg
    13081 cgcccacatg ggtaaacatc gccagagaga tatcgaaatg gttattagaa tggcagcccc
    13141 aggtcagccc ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa agcgtccaga
    13201 agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga cccatttcgc
    13261 gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc cgaaactccg
    13321 ccataacttc acgaccggag aaaccctgtt ctgcgccgca tggatcttcg gcataggtaa
    13381 gaacatcatt cagccctttg catagagaaa tggcttcatc aagtagccag gcaccgttgg
    13441 gatcaacggt aatccgcgca tcagggaagc gtttcttcaa tgcacgaaca gtgtcgattt
    13501 cttgctcgcc aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct
    13561 gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga tggcgcaact
    13621 gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt ttggtccgat
    13681 caccgatata aaacagataa ccgaggacgg taatagtctc gcgttgcttg cctggcccca
    13741 acagttcgca gaccggaaca ttcagcgcct tacctagcag gtcaagcaag gcggcttcca
    13801 gcgccgccac ggcattaacg tgcaattcaa aagtccaggc acctttgccg aaggtatcaa
    13861 aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg cgcgcaactt
    13921 cctggcccag aaccatcgga atagcatcga ccagtgtttg ataaatcacc tctccgcccg
    13981 gcgcttcacc aatgccggta tgcccggcgt tatcggtgag taccacaata ttgcgggtga
    14041 aatatgcgtt atgtgcccca ccaatattaa gcaacatgct atcgtgcccg gcgaccggaa
    14101 tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca
    14161 ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt
    14221 aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt gccaacgata
    14281 taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca
    14341 ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc
    14401 ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg
    14461 acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta
    14521 cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat cccacccagc
    14581 acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc tttcagaatt
    14641 gacatcccac gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg
    14701 gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagttgt
    14761 ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg ctgatccata
    14821 ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac ccccggatgt
    14881 tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc cagaccgccc
    14941 ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg
    15001 aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc
    15061 tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca
    15121 agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc actaaagata
    15181 tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg
    15241 cgttttgaac caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc
    15301 catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc
    15361 atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata
    15421 aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc
    15481 tgacttaaag aactcgttgt tgcactcctg aaaattcgcg ttagccacgc taactctgga
    15541 ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt tttataggtc
    15601 gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag
    15661 cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag
    15721 ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa
    15781 aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa
    15841 aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga ttcagcaccg
    15901 ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc cactgttcga
    15961 cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc aacagcattt
    16021 cgccaactcg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg ccgccattgc
    16081 agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga ttcggctgga
    16141 agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga aggtcgccct
    16201 gcccggtcgt ggacgtaacc accagaacat acttatcctg ataaggcagc cagtcgctta
    16261 attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt
    16321 cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca atttccgcca
    16381 taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg gcgcactgaa
    16441 ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata acgcctgcat
    16501 ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa
    16561 tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag caccgctgcg
    16621 attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca aatgggcaag
    16681 atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca ggccgtagcg
    16741 cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac cgcgataatg
    16801 cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat ttatcagcga ttttgtccag
    16861 ttcttccacc agtggataat ccagcaccgc ttcttccatc aaccagccgc gcacaatcgc
    16921 atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc gtccggcctc
    16981 gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat gagcagtaaa
    17041 aacatgctgt cctatctggt tacgcacggt ttgcatgacc actactttct cgtcgcgatc
    17101 cagccagcta cggtgaacca gccagccgga gggtttattt accgaaacca gccattcatc
    17161 ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc cagtttttcc
    17221 aactcagcca gaataagcgc gtgttgcgga tggtccgtcg ccagcgccat ttcataatag
    17281 ggtgcaacag caaaagcgcc cggtaacggc tggttgttat ttaacaaatc gtgcattcgc
    17341 gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa gaacggttgg
    17401 gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt
    17461 aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat aaagcaacct
    17521 caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac tgtattcaca
    17581 gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc tcatccttga
    17641 caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt cctgctcaca
    17701 ccaccccgat gtgatacttc atcctgaagt gtccttggcc ttcctgacca gccgaatcat
    17761 cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg cgtcatcctc
    17821 ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc
    17881 atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga atcgtctttt
    17941 tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca tatgaaagtt
    18001 aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat ctttttaagt
    18061 attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca caaccagatg
    18121 gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag atttgaggct
    18181 aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga gagattacac
    18241 accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg cggcagtttt
    18301 agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg gaagtcgtct
    18361 tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg catatccccg
    18421 gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt
    18481 tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta tacggcatct
    18541 tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact taacggccag
    18601 cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt
    18661 gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt gtcgtttagt
    18721 ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc acttgcgggc
    18781 ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccagcct actgcaaggc
    18841 gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct gccttttcac
    18901 ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg tttgccgaag
    18961 gtcgctgtcg gtcatgttga acttgattac accagcgtaa atctgattga gtcgaagagt
    19021 tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga tgaggtgcgc
    19081 cagacgctgg agcgcgactt aagcacttgc gctcagggta agattagcgt ggcgttatat
    19141 cgtcttgatg aactggaagg ccagccgata ggtcatttta atggcacttg cattgatgac
    19201 caggatatca ctatcgataa ctatgaattc actactgact atctggagaa tgccacctgt
    19261 ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa ctgcctgatc
    19321 acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca aattgacaga
    19381 gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca cgaacagtgc
    19441 gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt gagcgtttac
    19501 gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa tagcgatttt
    19561 gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg cgtgctggat
    19621 tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag ggaatgacga
    19681 tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg ctccatggat
    19741 atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga cctctatcaa
    19801 ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga taacagcaaa
    19861 gaattgctgt ctcgttttga aaatttcgat attaacgtct tgcgccgtga acgcggcgta
    19921 aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat tattcgcgct
    19981 ttgcaggcca acttgttcgc ggtcctgcgt gacattctct tcgtttacgg gcaaatccat
    20041 aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac taacctggtc
    20101 ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc
    20161 tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg cgtcggaaac
    20221 cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc gatggaagcg
    20281 ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag tcgttttatt
    20341 ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt caacgaattg
    20401 atcatcatgc cagatatcga aaaacgtctg gaagcgtttg tccgtatcgc tcacggtatc
    20461 attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct gggaatttta
    20521 atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc gaaagagagc
    20581 gccgactact tccgcgtact ggacgagttt gtcgtgcata cgctgggtga aaacgcgcgc
    20641 cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg
    20701 atgccgctgg tgaaagaaaa tcgccgcgac acaggcgatg cctacagctt taactggtca
    20761 atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa tatggctaat
    20821 ctgaagcttt acccggatca acccgttgaa gtgctagctg ccgacctgcg ccgtgcgttc
    20881 tccggtattg tggcgggtaa cgtaaaagca gtcggtattc gcgccattga agagtttggt
    20941 ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct gctacagggt
    21001 tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg ctacgaaatc
    21061 tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac
    21121 ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc acgcgacaaa
    21181 tatcatcaca gttaatatgt catacaattt atgttgcaac gcaaacgttt ctctattttc
    21241 ataaaccgta ttttatcgct atgaaaaaga aattatcgcc atgattaact aaaagtattg
    21301 attttttcag ttcaacctac atatattgcg cgccctggaa gaagtcagat gtcgtttaat
    21361 gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa gcgttgggat
    21421 aatctatctt ccaagtagat tattgtattt gagatcaaga tcactgatag atacataact
    21481 tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc atcgccgata
    21541 gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa aagctgaatt
    21601 atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga ttgcgtcgaa
    21661 agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc tttacggcac
    21721 ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc
    21781 gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc gcggcctgac
    21841 tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg tagaagaaca
    21901 ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta tctacccgat
    21961 cctgctggtt tatagcgtgg caatcaccaa tacagttgaa agcttcatgt ctcaccagct
    22021 gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac
    22081 catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg tgttcccgtt
    22141 tgttggcgta ttgatgctgc tggctctgta cctgatcccg cagtggaacg gcgcagcact
    22201 ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg
    22261 gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct cttctttcgc
    22321 cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga agatcctggc
    22381 attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct gtgtactgag
    22441 cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc tgtcttacct
    22501 ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg cgattatcgc
    22561 tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca acggtatggt
    22621 gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga accgtatcac
    22681 tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga gcatcctggg
    22741 tatgattgaa accctgggcg gtccaatcat cgcgatgatc ctgttcctga tgccgatgta
    22801 cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca acgtattcgt
    22861 tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca gctaagtcct
    22921 ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc ctatgattag
    22981 cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg ttggaccaat
    23041 gaaagcgggt aaacaattta ccgacgatct gattgcccgt aacctgctta aagacgtgac
    23101 ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc accacactga
    23161 tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata tcgattccat
    23221 ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa acggtcagca
    23281 tgaagtggag ttcccggttg atcagtgcat gaacttccac gccgacaacc tttctctgca
    23341 tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca gccagactta
    23401 ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc aggatagcgc
    23461 accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac attgtcagga
    23521 aaccgggctg tcactctctg gcctgatgat gaaaaacgag ctagcgctgc acagcaaaga
    23581 agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta ttgagcgcgg
    23641 tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact
    23701 acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg ttgttgactg
    23761 gatcaacatg tttgcactgg cagtgaacga agagaacgct gctggcggac gcgtggtgac
    23821 tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact acgacaagtt
    23881 tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca gtgccattgg
    23941 ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc agggtgaagt
    24001 tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg caagcccggc
    24061 gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga cgtgtgaccc
    24121 ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg cagtaaaagc
    24181 ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct gcctcgataa
    24241 agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc gcgaaacctc
    24301 tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca gaggcctcgt
    24361 tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca tcgaatgtta
    24421 cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta ttgtcgatgc
    24481 actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg aaacctgcca
    24541 gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg ccgtttttga
    24601 tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca aagcgggtcg
    24661 accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg cctttgagca
    24721 acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag ccgccacgct
    24781 ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag ataaaggcta
    24841 ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac gttggctgga
    24901 tgcaccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg attactgggg
    24961 acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc
    25021 cacgcagctg ctggtcgagt ttcagagtct ggaagggata tatgagaatc tggatgcggt
    25081 tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc tgtgccgcga
    25141 tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat tgcggttggt
    25201 acggtaacgg cgagccggat gcaaacgatt atcaacacat tgaatttaat gcacttatat
    25261 accggataag acgcggctag cgtcgtatcc ggcattatca catcagcgca tttaccaggc
    25321 ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc caccggtaca
    25381 aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta caccaacatc
    25441 acgcaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt caacagcggc
    25501 attacgcgcc tcttccagcc actttcacgc ccataacgcg cgcgatatcg cggtacttct
    25561 caccggtaaa gtcagcgtta tagcgcatga catgcggtaa caggatggcg ttcgcaacac
    25621 cgtgtggagt gttgtaaaac gcgcccagtg gatgcgccat accatgcacc aaccctaacc
    25681 caacattcga gaagcccata cccgcaacat actgcccgag cgccatttct tctccggcat
    25741 ccttatcacc agcaaccgat cctcgcagcg ccccagcaat gatttcaatc gctttaatgt
    25801 gcagtgcatc ggttagcgcc cacgcgccac gggtaatata cccctcaata gcatgagtga
    25861 gcgcatcgac acccgtcgca gctttcagcg ctggaggcat accatccatc atgtcagcgt
    25921 caataaacgc cacctgcggg atatcatgcg gatcaacgca aacaaacttg cgccgtttct
    25981 cttcgtcagt gatcacgtag ttaatggtca cttctgccgc agtacctgct gtggtaggaa
    26041 ttgccagaat cggtacactg ggtttattgg tcggggaaag cccttccagg ctacgcacat
    26101 cggcaaactc cgggttgttg ctgataatgc caatcgcttt acaagtatcc tgtggagaac
    26161 caccaccaat agcgatcagg taatccgcgc cgctattctg gaatacaccg agcccttctt
    26221 tgacgacagt aattgttggg ttgggcacta cgccgtcgta aatcgcccat gccagccctg
    26281 cagcatccat cttatcggtc actttcgcca ccacgccgca ttgcaccagc gttttatcgg
    26341 tgacgatcag cgccttctga taaccacggc gtttcacctc atcggttaaa gccccaacag
    26401 caccccgacc aaaccatgcc gtttcgttca gaatcattct gttagccatc atccttctcc
    26461 ttgttgcttt acgaaattac tcttcaatcc gtaacccata ggttttgaat ttctccagca
    26521 ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc cgtaatcgcc agggtcgtca
    26581 ggtaaagttg cgccagcact tcaacttcat gcgccagcca taacgctttt tccagattca
    26641 cctcacaagc gataagccca tgatgttgta acaaagttgc cttacgattt ttgagagcca
    26701 gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc ataaggcgcg caaggaatag
    26761 aattaccacc tgccgccgca atcatgtagt gaatagcggg gatcggtcgg ttaagaatgg
    26821 aaactgctgt gcaatgaacg gcatgattgt gaacaaccgc gttggcatcc ggtctgcttt
    26881 gataggccgc catatggaaa cgccattcgc ttgaggggag ctttccttcc tcatgtttac
    26941 cgttaccatc aataaaggca atatgcgact ccgtcagttt ttcatatgga atgcctgtag
    27001 gcgtaatcag catcccatcc tgataacgta cactgacgtt ccccgctgtc ccctggttca
    27061 gtcccaggcg ggtcatttcc aggcaagtgt caataatctg acgagcaagt ttatttcgtt
    27121 ccattagcta cctctctctg attcaaaaca gggcaataat gttgttcctt tcacactatt
    27181 gaattagccg tttaat
//
LOCUS       DAJTYD010000037        27174 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTYD010000037 DAJTYD010000000
VERSION     DAJTYD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.037"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..499)
                     /gene="ppa"
                     /locus_tag="OO585_001440"
     CDS             complement(<1..499)
                     /gene="ppa"
                     /locus_tag="OO585_001440"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6629075.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEI"
     gene            809..1765
                     /gene="ytfQ"
                     /locus_tag="OO585_001441"
     CDS             809..1765
                     /gene="ytfQ"
                     /locus_tag="OO585_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6629076.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            1905..3407
                     /locus_tag="OO585_001442"
                     /pseudo
     CDS             1905..3407
                     /locus_tag="OO585_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3421..4443
                     /locus_tag="OO585_001443"
     CDS             3421..4443
                     /locus_tag="OO585_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6629077.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4430..5425
                     /gene="yjfF"
                     /locus_tag="OO585_001444"
     CDS             4430..5425
                     /gene="yjfF"
                     /locus_tag="OO585_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6629078.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5458..6456)
                     /gene="fbp"
                     /locus_tag="OO585_001445"
     CDS             complement(5458..6456)
                     /gene="fbp"
                     /locus_tag="OO585_001445"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6629079.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6632..8005
                     /gene="mpl"
                     /locus_tag="OO585_001446"
     CDS             6632..8005
                     /gene="mpl"
                     /locus_tag="OO585_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6629080.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8088..8639)
                     /locus_tag="OO585_001447"
     CDS             complement(8088..8639)
                     /locus_tag="OO585_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6629081.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8733..10085
                     /gene="pmbA"
                     /locus_tag="OO585_001448"
     CDS             8733..10085
                     /gene="pmbA"
                     /locus_tag="OO585_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6629082.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10269..10655
                     /gene="cybC"
                     /locus_tag="OO585_001449"
     CDS             10269..10655
                     /gene="cybC"
                     /locus_tag="OO585_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6629083.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10700..11164)
                     /gene="nrdG"
                     /locus_tag="OO585_001450"
     CDS             complement(10700..11164)
                     /gene="nrdG"
                     /locus_tag="OO585_001450"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6629084.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11263..13401)
                     /gene="nrdD"
                     /locus_tag="OO585_001451"
     CDS             complement(11263..13401)
                     /gene="nrdD"
                     /locus_tag="OO585_001451"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6629085.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(13795..15450)
                     /gene="treC"
                     /locus_tag="OO585_001452"
     CDS             complement(13795..15450)
                     /gene="treC"
                     /locus_tag="OO585_001452"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6629086.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15500..16921)
                     /gene="treB"
                     /locus_tag="OO585_001453"
     CDS             complement(15500..16921)
                     /gene="treB"
                     /locus_tag="OO585_001453"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6629087.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17022..17987)
                     /gene="treR"
                     /locus_tag="OO585_001454"
     CDS             complement(17022..17987)
                     /gene="treR"
                     /locus_tag="OO585_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6629088.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18172..18225
                     /gene="mgtL"
                     /locus_tag="OO585_001455"
     CDS             18172..18225
                     /gene="mgtL"
                     /locus_tag="OO585_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6629089.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18366..21062
                     /gene="mgtA"
                     /locus_tag="OO585_001456"
     CDS             18366..21062
                     /gene="mgtA"
                     /locus_tag="OO585_001456"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6629090.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21268..21654)
                     /gene="ridA"
                     /locus_tag="OO585_001457"
     CDS             complement(21268..21654)
                     /gene="ridA"
                     /locus_tag="OO585_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6629091.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYETFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21727..22188)
                     /gene="pyrI"
                     /locus_tag="OO585_001458"
     CDS             complement(21727..22188)
                     /gene="pyrI"
                     /locus_tag="OO585_001458"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6629092.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22201..23136)
                     /gene="pyrB"
                     /locus_tag="OO585_001459"
     CDS             complement(22201..23136)
                     /gene="pyrB"
                     /locus_tag="OO585_001459"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6629093.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23140..23274)
                     /gene="pyrL"
                     /locus_tag="OO585_001460"
     CDS             complement(23140..23274)
                     /gene="pyrL"
                     /locus_tag="OO585_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6629094.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23554..23949)
                     /locus_tag="OO585_001461"
     CDS             complement(23554..23949)
                     /locus_tag="OO585_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6629095.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24080..24793)
                     /gene="bdcA"
                     /locus_tag="OO585_001462"
     CDS             complement(24080..24793)
                     /gene="bdcA"
                     /locus_tag="OO585_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6629096.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            24864..25457
                     /locus_tag="OO585_001463"
     CDS             24864..25457
                     /locus_tag="OO585_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6629097.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25602..26054
                     /gene="tabA"
                     /locus_tag="OO585_001464"
     CDS             25602..26054
                     /gene="tabA"
                     /locus_tag="OO585_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6629098.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26177..27124
                     /locus_tag="OO585_001465"
     CDS             26177..27124
                     /locus_tag="OO585_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005056533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6629099.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIIKF
                     VMNNPEGERADLSKVEIEITLSTFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNISQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILIDSLDDAKVNLTPVIDSGDFFKIPIY"
BASE COUNT         6491 a         6750 c         7203 g         6730 t
ORIGIN      
        1 cgatttcagc tttagcggct tctgcgtttt cccaaccttc aactttcacc cacttgcctt
       61 tttcgaggtc tttgtagtgc tcgaagaagt gagcgatttg cgctttcagc agttcaggca
      121 gatcgtttac gtctttaatg tgatcgtatt ctttgctcag cttgctgtgc ggaaccgcaa
      181 ccagtttcgc atcttcaccg gcttcgtcgg tcattttcag aacgccaacc ggacggcaac
      241 ggatcacaga accaggctgc agcgggtacg gagtcgggac cagtacgtca accgggtcac
      301 cgtccagaga cagggtgtgg ttgatgtaac cgtagttgca cggatagaac atcgcggtgg
      361 acatgaagcg gtcaacgaac agtgcgccgc tctctttgtc gatttcgtat ttgatcggat
      421 ctgcgttagc cgggatctca ataacaacgt agatgtcttc cggcagatct ttacccgcag
      481 ggacgttgag taagctcatg tctgtttcct ttaaaaatat gtggcaaaca agtgccgagt
      541 attatagcca actcgcgccg aatgtctttg cttgttttcg tcttcatttt cctttttcgc
      601 cagttttaag acggtataca gagcaggaaa atccataacc gtagccgcat ttttcatagt
      661 gagatgaaag cgattacaaa cttgtgatta acgttttatt tacttttttg aagtgtgatg
      721 taacgcaatc cgttacataa cgaattgtct atagtttttt cgcgaacatc ttttcaccaa
      781 taataattac cccgactagg acaaccctat gtggaaacgc ttacttgtag tctctgcagt
      841 ctcggcagcc atgtcgtcta tggcgttggc cgctccatta accgtaggat tttcgcaggt
      901 cggatcggaa tccggctggc gcgccgcaga aaccaatgtg gcgaaaagtg aggccgaaaa
      961 acgcggaatt acgttgaaaa ttgccgatgg tcagcaaaag caggaaaacc ggattaaagc
     1021 ggtacgttcc ttcgttgcac aaggggtgga tgcgatcttt atcgctccag tggttgcgac
     1081 gggttgggaa ccggtattaa aagaggcgaa agatgccgaa atcccggtct tcttgctcga
     1141 ccgttccatc gatgtgaaag acaaatctct ctatatgacc accgttactg ccgacaacat
     1201 cctcgaaggt aagttgatcg gtgactggct ggtaaaagaa gtgaatggca aaccatgcaa
     1261 cgtggtggag ctgcagggca ccgttggggc cagcgtcgcc attgaccgta agaaaggctt
     1321 tgccgaagcc attaagaatg cgccaaatat caaaattatc cgctcgcagt caggtgactt
     1381 cacccgcagt aaaggcaaag aagtcatgga gagctttatc aaagcggaaa acaacggcaa
     1441 aaacatctgc atggtttacg cccataacga cgacatggtg attggtgcaa ttcaggcaat
     1501 taaagaagcg ggcctgaaac cgggcaaaga tattctgaca ggttctatcg atggcgtacc
     1561 ggatatctac aaagcgatga ttgatggcga agcgaacgcc agtgttgaac tgacgccgaa
     1621 tatggcggga cccgcttttg acgcgctgga gaaatacaaa aaagacggca ccatgcctga
     1681 aaagctgacg ttaaccaaat ccacccttta cctgcctgat accgcaaaag aagagttaga
     1741 gaagaagaaa aatatggggt attgagggtt gctatgcctg atgccgattc gtaggccgga
     1801 taaggcgctc gcgccgcatc cggcgatggt gcactgaagc ctgatgcgac gcttaccgcg
     1861 tcttatcatg cctactggga gcacgcttta caccggggga aaccatgacg accgaccaac
     1921 accaggaaat cctccgcacc gaaggattaa gtaaattttt ccccggcgtc aaagcgttag
     1981 acaacgttga tttcagcctg cgccgtggcg aaatcatggc gctgctcggt gaaaacgggg
     2041 cgggaaaatc aacgctaatc aaagcattaa ctggtgtata ccacgccgat cgcggcacca
     2101 tctggctgga aggccaggtt atctcaccga aaaataccgc ccacgcgcaa caactcggca
     2161 ttggcaccgt ctatcaggaa gtcaacctgc tacccaatat gtcggtcgct gataatctat
     2221 ttataggccg cgaacccaaa cgcttcggcc ttctacgccg caaagagatg gaaaagcgcg
     2281 ccaccgaact gatggcatct tacggtttct ccctcgacgt gcgcgaaccg ctcaaccgct
     2341 tttcagtcgc gatgcagcaa atcgtcgcca tttgccgggc tatcgatctc tccgccaaag
     2401 tgctgatcct cgatgaaccc accgccagcc tcgacaccta ggaagtggag ctattatttg
     2461 gcctgatgcg tcagttgcgc gatcgcggcg tcagcctgat ctttgtcact cactttctcg
     2521 atcagagcta tcaggtcagc gatcggatca ccgttttacg caacggcagt ttcgtaggct
     2581 gtcgggaaac gcgcgagcta ccgcagatcg aactggtaaa aatgatgctg gggcgcgagc
     2641 tggataccca cgcgctacag cgtgccgggc gaacattgtt gagcgacaaa cccgttgccg
     2701 cgttcaaaaa ttacggcaaa aaaggaacga tagcaccgtt tgatctcgaa gtgcgccccg
     2761 gcgagatcgt cggcctggca ggcttgctgg gatccggacg taccgaaacc gccgaagtga
     2821 tcttcggtat caaacctgct gacagcggca cggcgttgat caaaggcaaa ccgcaaaacc
     2881 tgcgatcgcc acatcaggct tcggtactgg gcatcggctt ctgcccggaa gacaggaaaa
     2941 ccgatggcat catcgctgcc gcctcggtgc gggaaaatat catcctcgct ctccaggccc
     3001 agcgcggctg gctacgaccc atttcccgca aagagcagca agagattgcc gaacgcttta
     3061 tccgccagct tggcattcgc acaccttcaa ctgaacaacc gattgaattt ctctccggcg
     3121 gcaatcagca aaaagtgttg ctttcacgtt ggctactgac ccgaccgcaa tttctgatcc
     3181 tcgatgagcc aacccgtggc attgatgttg gtgcccacgc cgagatcatc cgcctgattg
     3241 aaacgctatg cgccgatggt ctggcgctgc tggtgatctc ctccgaactg gaagaactgg
     3301 tgggctatgc cgaccgggtg atcatcatgc gcgatcgcaa acaggtggcg gagatccggc
     3361 tggcagagct ttccgttccg gctatcatga acgccattgc ggcgtaagga gaacagtgtg
     3421 atgcctcaat ctcttccgga caccactacg ccaaaaaggc gctttcgctg gccgacgggg
     3481 atgccgcagc tggtagcact attgctggtg ctgctggtcg acagcctggt agccccgcat
     3541 ttctggcagg tagtgcttca ggatgggcgt ttgttcggta gccccataga cattcttaac
     3601 cgtgcagccc ccgttgcgtt actggcgatt ggtatgacgc tggtgatcgc caccggtggg
     3661 attgatctct ccgtaggggc ggtgatggct atcgccggag ccacaacggc tgcgatgacg
     3721 gtcgcggaag tcagcctgcc gattgttttg ttaagcgccc taggcaccgg catcctggcg
     3781 ggattgtgga acggcatact ggtagcgatc ctcaaaattc agccatttgt cgccaccctg
     3841 atcctgatgg tcgccgggcg cggcgtggcg caactgatca cctccggaca gatcgtcacg
     3901 tttaactcgc cggatctctc atggtttggc agtggatcgc tgttgttcct gccaacgcca
     3961 gtcattattg cggtactgac gcttctcctg ttctggctgt tgacccgcaa aacggcactg
     4021 gggatgttta tcgaagccgt tggtatcaac attcgggcgg caaaaaatgc cggggtaaac
     4081 acgcggatca tcgtcatgct cacttatgtg ttgagcgggc tgtgtgcggc gattgcgggc
     4141 attatcgtgg cggcggatat tcgcggtgcc gatgccaaca acgccggatt atggctggag
     4201 ctggacgcca ttctcgcggt ggtgattggc ggcggatcgc tgatgggcgg gcgctttaac
     4261 ctgctgctct cggtagtggg ggcgctgatt attcagggga tgaacaccgg aattttgctt
     4321 tcgggctttc caccggagat gaaccaggtg gtgaaagcgg tggtggtgct ttgcgtgctg
     4381 attgtccagt cgcaacgctt tatcagtctg attaaaggag tacgtagccg tgataaaacg
     4441 taatttgcca ctgatgatca ccatcggcgt ctttgtgctg ggctatttct actgcctgac
     4501 gcagtttccc ggttttgctt ccacaagagt gatctgcaat atcctgaccg ataacgcttt
     4561 tctggggatt attgccgttg gtatgacctt tgtgatcctc tctggcggta tcgatctctc
     4621 cgtcggttcg gtgatcgcct ttactggcgt gtttctggca aaagtggttg gcgatttcgg
     4681 cctctcgccg ctgctggcgt ttccgctagt gctggtgatg ggctgtgcct tcggcgcatt
     4741 tatggggctt ctgatcgacg ccctgaagat cccggcattt atcattacgc ttgcggggat
     4801 gttctttttg cgcggcgtca gctatctcgt ttcggaagag tcgattccga taaaccatcc
     4861 catttatgac acgctctcaa gccttgcgtg gaaaatcccc ggcggcggtc gccttagtgc
     4921 gatggggctg ttgatgctgg cggtggtggt tatcggtatt ttccttgcgc atcgtacccg
     4981 ttttggcaat caggtgtacg ctattggcgg caacgcgacg tcggcgaacc tgatggggat
     5041 ttctactcgc agcactacta ttcgcattta tatgctctcc accggactgg caacactggc
     5101 ggggattgtc ttctcgattt atacccaggc cggatatgca ctggcgggcg taggtgtgga
     5161 actggacgcc atcgcgtcgg tggtgattgg cggtacgttg ttaagtggtg gcgtcgggac
     5221 ggtgcttggg acgctttttg gcgtggcaat tcagggactg atccagactt acatcaactt
     5281 tgacggcacg ctgagttcct ggtggacgaa aatcgccatc ggtattttgt tgtttatttt
     5341 tatagcatta cagcgtgggt taacggtgct gtgggagaat cgtcagagtt cgccagtgac
     5401 aagagtcaac attgcgcagc gataaaaatg cctctccgtg tggagaggcg caggagatta
     5461 cgcgtccggg aactcacgga taaagcgttc gacatcttca accatatggt cgttgccgac
     5521 aaagaacgaa cggcgctggt gcagagtttc cgggatgata tccagaatac gctctttgcc
     5581 atcgctcgct ttaccgcccg cttgttccgc caggaatgcc atcgggttgc actcataaag
     5641 caaacgcagt ttgccgtccg ggtggctggc ggtgcttggg tagagataaa taccgccttt
     5701 cagcaggtta cggtggaaat ccgcaaccag tgaaccgata taacgtgagg tatacgggcg
     5761 gttggtggat ttatcttctt cctggcagaa tttaatgtac ttcttcaccc cgttcggaaa
     5821 cttaatgtag tttccttcgt tgatggagta ggttttgcct ttctccggga agcgcatccg
     5881 ttcctggcac aggcagaaaa cgcccagcga aggatcgtaa gtaaaggcgt gaacaccgca
     5941 tccggtggtg taaaccagca tggtagagga gccgtatacc acgtaacctg ccgcaacctg
     6001 tttgttacca ggctggagga aatcttcttc cgttaccggc gtgccgacgg gcgtaactcg
     6061 gcggtagatg gagaaaatgg taccgacaga gacgttaaca tcgatgttgg acgagccatc
     6121 cagtgggtcc atcagcacca cgtattttgc atgttcacag ccttcaaaga caacaatctc
     6181 atcttcttct tcagaggcaa tgcctgcaac gatatcgcgt gctttcagtg cggctttcag
     6241 tttttcatta gcgaacaagt cgagtttttg ctgaacctcg ccctgcacgt tctcagcacc
     6301 gctggcaccc aggatatcaa ccagtcctgc tttgttgata tcgcgatgga taatcttggc
     6361 gcccagtttt attgccgaca gcaaagcagt gagctcaccg gtagcatgag aaaactcgtg
     6421 ctgcttttcg acaataaatt cacctaacgt tttcataaaa ctttccctgc aatgtttatg
     6481 gagtaaagcg accgcaacaa tcttaacaaa taatctcaat gttgcgctca ggtgaatcgc
     6541 gccagcaaat tacggattat cctgaaatgc gtttctcact tgcccgacat atgcgtaaaa
     6601 tgagcggcag attaaaaaag gatagtgacg tatgcgcatt catattttag gaatttgtgg
     6661 cacgtttatg ggcggtctgg cgatgctggc gcgccagtta ggccatgaag taacgggttc
     6721 ggacgccaat gtgtatccgc cgatgagcac cttacttgag aagcaaggca ttgaactgat
     6781 tcagggttac gatgccagcc agctcgatcc gcagccggat ctggtgatta ttggcaacgc
     6841 catgacccgt ggaaatccgt gtgtggaagc ggtactggaa aaaaacatcc cttatatgtc
     6901 aggtccacag tggctgcacg attttgtgct gcgcgaccgc tgggtgctgg ccgttgccgg
     6961 tacacacggc aaaaccacca ccgcgggaat ggcgacctgg attctggaac agtgtggtta
     7021 caaaccggga tttgtgatcg gcggtgtgcc ggggaacttt gaggtttcgg cgcgtctggg
     7081 cgaaagcgac ttctttgtta tcgaagcgga tgagtacgac tgcgccttct tcgacaaacg
     7141 ctctaaattt gtccattact gcccgcgtac gctgatcctc aacaaccttg agttcgatca
     7201 cgccgatatc tttgacgacc tgaaagcgat ccagaaacag ttccaccatc tggtgcgtat
     7261 cgttccgggg cagggccgta ttatctggcc ggaaaatgac atcaacctga aacagaccat
     7321 ggcgatgggc tgctggagcg agcaggagct ggtgggtgag caggggcact ggcaggcgaa
     7381 aaagctgacc actgatgctt ccgaatggga agtcttgctg gatggcgaaa aagtgggcga
     7441 agtgaaatgg tcgctggtag gcgaacataa tatgcataat ggcctgatgg cgattgcggc
     7501 ggcccgccat gttggcgtag cgccggcaga taccgctaac gcgctgggtt cgtttattaa
     7561 tgctcgtcgc cgtctggagt tgcgtggtga agcgaatggc gtcacggtat atgacgattt
     7621 tgcccatcac ccgacggcga ttctggcaac gctggcggcg ctgcgtggca aagttggtgg
     7681 tacagcgcgc attattgctg tgctggaacc gcgctcgaat accatgaaaa tggggatctg
     7741 caaagacgat ttggcacctt cattaggtcg tgccgatgaa gtcttcctgc tgcaaccggc
     7801 gcatattccg tggcaggtgg cagaagtggc agaagcctgc gttcagcctg cacactggag
     7861 tggcgatgtg gatacgctgg cagatatggt ggtgaaaacc gctcagcctg gcgaccatat
     7921 tctggtgatg agcaacggcg gttttggtgg gatccatcag aaactgctgg atggtctggc
     7981 gaagaaggcg gaagctgcgc agtaattcgg cctcagcctg agatagcatt gccggataag
     8041 gcgtttacgc cgcatccggc aaaccatact cacatcaaca acgaaaatta ctcttcattc
     8101 tccgccagtt cgcgcagata ctggaaaatc tggcgtgcgg atttcggcgg cttattccct
     8161 tctttctctt tcttcgcgtt gcggatcaga gtacgcagtt gctgacgatc ggcatcgggc
     8221 cacagattta atacctcagc gatggcgtca tcaccttgat cgatcagacg atcgcgcagg
     8281 ttttccagtt tatggaacag caccacctgc tggttgtgac ggtttttcag cttatccagc
     8341 gcctgacgga tcggttctac atcgcgctgg cgcagcatct taccgatgag ctgcaactgg
     8401 cggcggcgac cttccatctt aatacgctgg gcgagctcaa tagccgcgcg cagatccgca
     8461 tctaacggga tcttatccag cgcgtttttc cccagatcaa caatttccgc gccaaggcgt
     8521 tttagctcct cggcatcacg tttaatttca cttttactga cccagataat ttcatcgtct
     8581 tcgtcttcga tgtcatcacc gggaacgtcg tcgagccagt cttcgggctg cttagtcatc
     8641 tcaggctcct taaaaaaaga ggctaatgtt accagttaag atgcgcactg aaaaacggtt
     8701 ctctgttaga cttcagagaa actctctaca ttatggcact tgcaatgaaa gtaatctctc
     8761 aagttgaagc gcagcgcaag attctggaag aagcagtttc cactgcgctg gagttggcct
     8821 caggcaaatc ggacggtgcg gaagttgccg tcagcaagac caccggcatt agcgtaagca
     8881 cgcgttatgg tgaagtggag aatgttgaat tcaatagcga tggcgcgctg gggatcactg
     8941 tttatcacca gaaccgcaaa ggtagcgcat catccaccga tttaagcccg caggccattg
     9001 cccgtactgt acaggcggcg ctggatattg cccgttatac ctcgccagat ccctgtgccg
     9061 gcgtggcaga caaagagctg ctggcctttg acgcaccaga tctcgacttg ttccaccctg
     9121 cggaagtttc cccggatgaa gccattgaac tggcggcccg cgcagaacag gcggcattgc
     9181 aggcggacaa acgcatcacc aataccgaag gtggcagctt taacagccac tacggtgtca
     9241 aagtttttgg caacagccac ggcatgttgc agggttactg ctcaacgcgt cattcgctct
     9301 ccagctgtgt aattgccgaa gaaaatggcg atatggagcg tgattacgcc tacaccattg
     9361 gtcgtgcgat gagcgatctg caaacgccag agtgggttgg ggtcgactgt gctcgccgta
     9421 ctttatcgcg cctgtcaccg cgtaaactct ccaccatgaa agcgccggtc atttttgcca
     9481 atgaagtggc aaccgggctt tttggccatc tggtgggggc gatagcgggt ggatcggttt
     9541 accgtaaatc taccttcctg ctggattcgc tgggtaaaca aattctgccg gactggctga
     9601 ccattgaaga gcatccgcat ctgctgaaag ggctggcgtc gacgccattc gacagcgaag
     9661 gtgtgcgcac cgagcgtcgc gatattatta aagatggcat cctgactcag tggctgctga
     9721 ccagctattc ggcgcgaaaa ctggggctga aaagcaccgg acatgcgggc ggtattcaca
     9781 actggcggat tgccggacaa ggtctaagct tcgagcagat gctcaaagag atgggcaccg
     9841 ggctggtggt gacggaattg atgggccagg gcgtgagtgc aattaccggt gattattccc
     9901 gtggtgcggc gggcttctgg gtagaaaacg gcgaaattca gtatccggtt agcgaaatca
     9961 ccatcgcagg taatttaaaa gatatgtggc gcaatattgt caccgtcggt aacgatattg
    10021 aaacacgcag taatatacag tgtggttctg tgctgttgcc ggagatgaaa atcgccggac
    10081 agtaaaaaaa ctggcgcgac ctgtcgcgct ataaactaaa aaattacaca aaatcattcg
    10141 cactgcatcg agacggaaag tgaatgaggg ccggggagcg tactcgcagt acgtgacccg
    10201 gtcgaatgag cgtagtcgtc gatgaggcag tgtgaatgat gaagtgtaaa taaaaaagga
    10261 agtgagcaat gcgaaaaagc ctgttagcta ttcttgcagt ctcctcgttg gtattcagtt
    10321 cggcgtcgtt tgccgctgat cttgaagaca atatggaaac cctcaacgac aatttaaaag
    10381 tggtcgaaaa agccgataac gcggcgcaag tcaaagacgc gttaacgaag atgcgcgccg
    10441 cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga aggcaaatca ccggacagcc
    10501 cggagatgaa agacttccgt cacggtttcg atattctggt cggtcagatt gacgacgcgc
    10561 tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc tgccgcagag caactgaaaa
    10621 cgacccgcaa cgcctatcac cagaagtatc gttaattcct catttccctg ttgcctgcac
    10681 tcaggtaaca gggaacttat catcgcaaat gatgcaccac ctgattgctg ctgccgcgcc
    10741 agatcaggga agggtctttt aaatcctgca caaatttgcc gtcgaccagc acgttaatca
    10801 aatcaacaac ctgcatttgc gctgcgttga gttcgtcgag tttatagcct gtccacaccc
    10861 agatgtcttt acccggacac tcggcgcgga tgcgttgtac cagtttcaga atatccggca
    10921 cgttttgcgg atgcagcgga tcgccgccgg agagggaaat accctggcgt ttgatacgag
    10981 tgtcattcag atcgttaatg atctggtctt ccattgcttt agtaaatggc tgaccggaat
    11041 ttacccgcca tgtgcttttg ttatagcaac cggggcattc atgaacacac ccggagacaa
    11101 acagagtgca acgagtgccg gggccgttga cgatgtcgac aggatagtac tgatgataat
    11161 tcattatttc gcctggctta tcagtccgcg tgtagactgc atccggtaat caatgcctga
    11221 tgcgacgctt tcacatcaca tccggcattt gcttagtgca gatcaaccta tctgcccatt
    11281 tcccaaatgt ttaacgcggc gcttaacttc ttcctgcttg ccggcgttaa acggacgtgc
    11341 atccgggcta cctaaatatc cgcacacgcg gcgagttacc gacacacggg aggcgtcatg
    11401 gttaccacat ttcgggcagg tgaagccttt gctggtgcac tcgaactcac cggtaaagcc
    11461 acactcgtag cactcatcaa tcggtgtatt ggtgccgtaa tacggtacat gctgatagct
    11521 gtaatcccag acatcttcca gcgccttcag gttgtgctga atgtttgaat actcgccgta
    11581 gcaaatgaaa ccaccgttcg ccagcggcgg gtaaggcgct tcaaagtcga tcttgtcgta
    11641 cgggttcacc ttcttctcca catcgaggtg gaaactgttg gtgtagtaac ctttatcggt
    11701 cacgcccggc accacgccaa actcagcagt atcgagacgg cagaagcggt cgcacaggtt
    11761 ttcactcggc gtgctgtaga gactgaaacc ataacccgtt tcttccttcc actgatccac
    11821 tgcctgacgc agacgttcaa caatggcgat acctttcgcg cgaagctgct cgttgtcgta
    11881 aacatgctcg ccgccgaaca gcgcgttaat ggtttcgtgg atgccgatgt aacccagcga
    11941 aatagacgca cgaccgtttt tgaagatttc agaaacatca tcgtcagcat tgagacgcac
    12001 gccacaagca ccttccatat agaggatcgg ggccacgcgc gctttcacgc cttcgagacg
    12061 agcgatacgg gtcatcagcg ccttacgtgc cagcaccaga cgttcatcca gcagcttcca
    12121 gaaggtggct tcatcgcctt ttgcttccag agcaatacgc ggcaggttca ggctgatcac
    12181 gccgaggttg ttacgaccat cgtggatctg ctcgccgttt tcattttccc acacgccgag
    12241 gaagctgcgg cagcccatcg gagttttaaa cgaaccggtg actttcacta cctgatcgta
    12301 gttcaggata tccggataca tgcgcttgct tgcgcactcc agcgccagct gtttgatgtc
    12361 gtagttcgga tcgccttttt tatggttcag gccatcgcga atcgcaaaca ccagtttcgg
    12421 gaacaccgca gttttacggt ttttaccgag gcctgcgata cggttacgca ggatagattc
    12481 ctgaatcagg cgcgactccc agctggtgcc cagaccaaaa ccaaaggtta caaacggcgt
    12541 ctgaccgttg gcggtgtgca gggtgtttac ttcgtactcc agcgactgga aggcatcgta
    12601 gcactcttta atggtacgag agttagcgta gccttcggcg tccgggatgt tccactcttc
    12661 tgcggtcttg cgatgtttgt tgtagctggc ggtgacaaac ggtgccagca cttcatcgat
    12721 acggttaatg gtggtgccgc cataaatatg gctggcgacc tgagcaataa tctgcgcagt
    12781 tacagcggtt gccgtagaga tcgacttcgg cggttcaatc tcggcgttcc ccatcttaaa
    12841 gccctgggtc agcatgcctt tcaggtcgat caacatgcag ttgaacatcg ggaagaacgg
    12901 cgagtaatcg agatcgtgat agtgaatatc gccacgctca tgtgcctgca ccacgtcacg
    12961 cggcagcagg tgctgacgtg catagtgttt agccacgatc ccggccagca ggtcgcgctg
    13021 ggttggaatc accttgctgt ctttgttggc gttttcgttg agtaacgagg cgttggtctg
    13081 ctcgaccaga ccacggatct cctgattcag gcgaccgcgt ttttcacgtt caatgtcgcg
    13141 atcgtgacgg tactcgatgt aagcacgagc cagttgtttg tatggacctg acatcagctg
    13201 gttttcaaca gcggtctgga tctcattgat atccacctgg ttgcggccct gcatctgctc
    13261 gctgacaact gcagcaacag tggcgcaata atcggcatca tcgacttccg ctgctttagc
    13321 tgcacgcaga atcgcttctt tgatgcgctc tgatttaaac ggcactttgc agccgtctcg
    13381 tttcatcaca tgcggtgtca tgatcgctcc atatttttaa gaacaggtta tccacagaaa
    13441 ttgggaaagg cgttttccgg ttttttcgtt tcgttttccg gtgctttccg caatcgccat
    13501 ccgcttttcc acaacgccgc acctttttcg tggggagctg tagtagcaat tatagtcgat
    13561 taatacaaca tattgggttg ggacgcattt taaagtctat atatagtgct ttgcatcaag
    13621 gatgtttgaa ctttttttga tgtagctcaa agtaaaaagc agagcgtacg gatgacgggc
    13681 gctacagcgt tatgtaaatt ttttaatgaa tttgctggtt gaaaaataaa caaaaacaac
    13741 atgctgacag acaaaacccc ggaatgaccg gcattaccgg ggcttaggga agatttactt
    13801 ctgtaaccac cagacagcct caaaaggccg taaattcatg gcacagggtt gtggtgaggc
    13861 ttcttcgtag ttatgcatca caagctgcca gttgccgcgc atttgccctg gctgccaggg
    13921 ttggatctca cggctaaggt tggcaatgac cagcaaggtt tgccccttcc attcacggcg
    13981 atagcaccac aatacagggc tgtttggcag cagatcctgg taattgcccc atgtcaggat
    14041 ggcttcctgc ttacgcagtg cgattaactt ttggtaggtg taaaacaccg aggaatcatc
    14101 ggccagcgcg gcttctacgt tgatttgttg atagttatca cccaggccaa tccacggttc
    14161 gccagccgta aatccggcat tatcgccgtt gctccattgc atgggcgtgc ggctgttgtc
    14221 acgggattta ctggcgagga ttgccaataa ctcgtcggca tcacgaccat cgttgcgcag
    14281 ctcggcaaac atattgaggc tctctacgtc gcgatagtca gtaatgcgcg tgaaatgcgg
    14341 gttggtcatg ccaatctctt cgccctggta gatatacggc gttccctgca tgccatgcag
    14401 caccatcgcc agcatttttg ccgcaggcac gcggtattca ccttcatcac caaagcgaga
    14461 aacaatgcgc ggctgatcgt ggttacacca gaacaaggca ttccatgcta cgttgtgcat
    14521 tccttgttgc cagtggcgga acaatgtttt caacgccaca aagtcaggtt tagccagcgt
    14581 ccatttttca ccaccgggat aatcgacctt caggtgatga aaattaaagg tcatcgacaa
    14641 ttcactgcct gtcagtgccg cgtatcgctg gcaatgctca aggctggtgg aggacatttc
    14701 acctacggtc attaacccgc gtggcgtaaa cacatcgcgg ttcatctcgt gcaaaaactc
    14761 gtgtgctcgt ggcccgtcgg tgtagaagcg acgcccgtcg ccatccaggt catcagggaa
    14821 acgcgggtct ttggagatca gattcaccac atccaggcgc aacccgtcga ccccacgatc
    14881 ggcccagaac tcacagactt ttttcagcac tgcgcgtacc gctggattct cccagttgag
    14941 atccgcctgt tctggtgcaa agagatgcaa atagtactgt tcgctttccg catgccagcg
    15001 ccacgcacta ccgccaaatt ttgaacgcca gttgttcggt ggcgtttctg gttctccatc
    15061 gcgccagata taaaactggc ggtaagggct ttctttgttc agcgcctcgc gaaaccaggc
    15121 atgttgggta gaggtatggt taaacaccat atcgagaatg atacgaatcc cgcgcgattt
    15181 tgcctgcgtc accagttcgt caaaatcgtc cagcgtgccg taggtgggat caatcgccgt
    15241 atagttcgct acgtcgtaac cgttatcgac ctggggagag acataaaagg gggttagcca
    15301 gatggcatca acgcccagtt tatgcagata gtccaggcgt tggataacgc cacgtaaatc
    15361 gccggtaccg ctacccgtgg tgtcctgaaa actctttgga taaatctggt agataacgcc
    15421 gttttgccac cagtggggag gattagtcat tacgttattc ctgcaaatgc gagggggcgc
    15481 aattgcgccc cgaagaaaat taaacaatgt ccagcgtgcc caggcggtat ttccgctgat
    15541 agataaacga ggtgagtaca atcgggatga tgatggcgat agccattgcc agcgcaaaca
    15601 cctgccagta gttcggttga atcgagagaa ttcccggcag gccgcctacg ccgatgccat
    15661 tcgccataac gccgttcagg ccgcatagca atcctgccag accagaacca atcatcgcgc
    15721 acagcatcgg gaagcgatat ttcaggttga tgccgtacat tgcaggctca gtgaccccaa
    15781 gccaggcgga gatcgccgca ggcacggaga tctcgcgttc attgtgcttg cggctggaaa
    15841 tgataatgcc gatcacggcg gagccctgag cgatattcga cagcgcaatc agtggccaca
    15901 ctggcgtacc acccatgctt tgaatcatct gcaaatcaat agcaagcgtg gtctggtgta
    15961 ccccggtgat caccagcggg gcgtacagga agccaaacaa tgccgcgcca atcggagcaa
    16021 agctgccggt catcaggtga cgtaccgcaa aggcaacgcc atcgccaatc atgcgaccaa
    16081 acggaccaat cagcgcatgg gcgaggaaca ccgcgaggat cagcgaacag acgggcacca
    16141 ccaccagata gaggtaatct ggcacgatgc gtttaaggcg agtttcaata acgcccagcg
    16201 ccagcccggc taacagtgcc gggatcacct gcgcctgata gcccactttg gcgatgctga
    16261 acatgccaaa gtcccacact tccggcagct gctgcccgag cagataagcg ttcatcagtt
    16321 ggggggaaac cagtgtcacg ccaagcacga taccaaggat cggtgtgccg cccatttttt
    16381 tcaccgctga ccagcaaatc ccgaccggca ggtagaagaa gatcgcttca ccgatcaacc
    16441 acagaaaatc gtagatcgtt tgcagggaag ggtacatttg cgccagcgtt tgaccgttgc
    16501 tcatgggcaa atcgccgatc acattgcgaa aaccgaggat caaaccgccg ctaatcaacg
    16561 cgggcagcaa cgggaagaag gtctctgcga aatgagagat caactgctca tgccatttca
    16621 tattctgccg ggcggctttt tttacctgct ctttatcaac ctgcgcctgt ccggttgacg
    16681 ctatcagtgc ttgatagtag tcacccacgt tggtgccaat caccacctga aattgcctgg
    16741 cattggtgaa acagcctttc accatgggga gttgctcaat ttctttcggt ctggcattgg
    16801 ccggttggtt gaggacaaag cgtaggcgag taatacagtg gctcaccgtc gcaatattgc
    16861 cgcgcccgcc gaccagttca atcaaccgat cgatatccgt ttggtttatt ttgctcatca
    16921 taaagcccca tggcagatga catttttggt tggctgcaga atatattgcg cggaaaaatt
    16981 taaaaacggg aacgttccct aaacgcagcg aagatcacaa tttatcgttc aggaaacgat
    17041 ctggacaggg tggcggggat gatgatttgt tgcggttcgc tgcgcccggt tacctgtgcg
    17101 atcaactggc aggccgcctg gcgtccagct tcggcgtaac cgggatctac ggtgacgatc
    17161 tccggatgga ggaatttcat taacggcgta ttaccgacgc tcgccagttg caaggtgtcg
    17221 atgcgttgct cttgcaggta tttacttgcg ccaagtgcca gcgtgtcggt tgcgcacagt
    17281 aaggcggtag tttcaggcgt aatcactttt gctacgttct catagccttg cttcatagca
    17341 aggcctggca gagcggcaac gggatgcagt ttatgcgctt tgcagaacgc caggtaggct
    17401 tcgtgacgtc gcttaccggt cgtcacgtca ctgtgcggca cgccgagata actgatatta
    17461 cgatgcccct ggtcatacag ccgttgcatc aggatcttga ttgccccttc gtcgtcataa
    17521 cagaccgaag caaagccttt tgcgtcacgc gccagcagaa ccagcgatga ctgccagtgg
    17581 gctaacattt cttctgttat gccggtaaaa ccgaacagca ctacgccgtc gatattacgc
    17641 cgtttcagca cccccaaatg ttcggcaact aattgcgggg aaaactgact ttccatcatg
    17701 attgggtcgt aaccttgttc atagaactct ggcagcatgg tttgaacggc gagattttct
    17761 gacaacgaat ccaggcgggt aacaatgatg gcgaccactt tatcgctttg cccacgcata
    17821 gcgcgcgcag agcgggaagg ggaaaatcca tgctgattca tcactgcttc aacacgctcg
    17881 cgggtgcgct ggctcacgcc gctttcgtta ttcagcaccc gggaaactgt agatttcccc
    17941 acgccgctta agcgcgcgat gtctttgatg gtcagccgat tttgcatcct gttgtcctgt
    18001 aacgtgttgt ttaattattt gagcctaact ttacccgtgc attcagcaat gggtaaagtc
    18061 tggtttatcg ttggtttagt tgccagcagg tattatatcg ccatagatgc tacgaatatt
    18121 attggattct ccttattatt tgcggcgctt ttttcactta ccggaggtta tatggaacct
    18181 gatcccacgc ctctccctcg acggagatta aaacttttcc ggtaagcccg tcttttcacg
    18241 gcgttaccgg atgcgtaagg ccgtgacgtt ttaacgtccc tgctcagctt tattaccttc
    18301 aggtaaggct tcgccacgcc tgaagacatt tctgtactgt ttcagacagt gcggagggac
    18361 tccttatgtt taaagaaatt tttacccggc tcattcgcca tttaccttcc cgtctggttc
    18421 atcgtgatcc attgcctggc gcgcagcaga cagtgaatac ggtggtcccg ccgtccttaa
    18481 gtgcgcattg cctgaaaatg gcggtgatgc ccgaagaaga attgtggaaa acgttcgaca
    18541 cccatccgga agggttaaat caggcggaag tggaatctgc ccgcgaacaa catggtgaaa
    18601 ataaattacc cgcacaacaa ctgtcgccgt ggtgggtaca tttatgggtc tgctatcgca
    18661 acccctttaa tattttactc accattctcg gcgctatttc ttacgccacg gaagatttat
    18721 ttgctgcggg cgttatcgcg ctaatggtcg ctatttctac gttgctgaac tttattcagg
    18781 aagcacgttc cactaaagcg gcagatgccc tgaaagcgat ggtcagcaat accgcgacgg
    18841 tgctgcgcgt aattaacgac aaaggcgaaa atggctggct ggagatcccg atcgaccagc
    18901 tggtgcccgg cgatattata aaactggcgg cgggggatat gatcccggca gatttacgta
    18961 tcttgcaagc gcgggatctg ttcgtcgctc aggcgtcgtt aaccggtgag tctctgcccg
    19021 tggaaaaagc cgctaccact cgccagccgg agcacagcaa tccgctggag tgcgacacgc
    19081 tgtgttttat gggcaccacc gtggtgagcg gcacggcaca agcaatggtg attgctacag
    19141 gtgccaatac ctggtttggt caactggcgg ggcgtgttag tgagcaggaa agcgagccga
    19201 atgcctttca gcaagggatc agccgcgtca gtatgctgct gattcgcttt atgctggtga
    19261 tggcaccggt ggtgctgtta atcaatggct acaccaaagg cgactggtgg gaagcggcgc
    19321 tgtttgcgct ttcggtcgcg gtaggcctaa cgccggaaat gttgccgatg attgttacct
    19381 cgacgctggc gcgcggcgca gtaaagctgt cgaaacagaa agtgatcgtc aaacatctgg
    19441 atgctattca gaactttggc gcaatggata ttctgtgcac tgataaaacc ggcaccctga
    19501 cgcaggataa aattgtgctg gagaatcata ccgatatctc cggtaaaacc agcgaacgcg
    19561 tgctgcatag cgcgtggttg aacagtcatt atcagaccgg acttaaaaac ctgctcgata
    19621 cagcggtgct cgaaggtacg gatgaagagt cagcgcgctc gctggccagt cgttggcaaa
    19681 aaatcgatga gattccgttt gatttcgagc gtcgccggat gtcggtggta gtggcagaaa
    19741 ataccgagca ccatcagctg gtttgcaaag gtgcattgca ggaaatcctc aatgtgtgtt
    19801 cgcaggtgcg tcacaatggc gagattgtgc cgctcgatga catcatgctg cgtaagatta
    19861 agcgggttac tgatacgctg aatcgtcagg ggctgcgcgt ggttgcggtg gcgacgaaat
    19921 acctgccagc gcgtgaagga gattaccagc gggcggatga atccgacctg atcctcgaag
    19981 gatatattgc ttttcttgat ccgccaaaag agacaaccgc tccggcactg aaggcattaa
    20041 aagcgagtgg aattaccgta aaaatcctta ctggcgacag tgagttagtt gcagcgaaag
    20101 tgtgccatga agtggggctg gacgcgggag aggtggtcat tggtagtgat attgaaacgc
    20161 tatctgacga cgaactggca aatcttgcac agcgcaccac gctgtttgcc cgcctgacgc
    20221 cgatgcataa agaacgcatt gtgaccttac tgaagcgcga agggcatgtg gttggcttta
    20281 tgggcgatgg tattaatgat gcgcccgcct tacgcgcggc ggatatcggc atttctgtgg
    20341 acggcgcggt agatattgcc cgtgaagcgg ctgatatcat cctgctggaa aaaagcctga
    20401 tggtgctgga agagggggtt attgagggac gtcgcacttt cgccaacatg ctgaaataca
    20461 tcaaaatgac ggcgagttct aactttggta atgtgttcag cgtgctggtg gcgagtgctt
    20521 tcttgccctt cctgccgatg ttgccgttac acttgcttat tcagaacctg ctgtacgatg
    20581 tgtcacaggt ggcgatcccg tttgataacg tcgacgacga gcaaattcaa aagccgcagc
    20641 gttggaatcc ggcggatctg gggcgcttta tgatcttctt cggaccgatc agctcgatct
    20701 tcgatattct gacgttttgc ctgatgtggt gggtattcca tgccaacacg ccggaaacgc
    20761 aaacgctgtt ccagtcggga tggtttgtgg tgggcttact gtcgcaaacg ctgattgtgc
    20821 atatgatccg cacccgccgt gtgccgttta ttcagagctg tgcatcgtgg ccgttaatga
    20881 tcatgaccgt gatcgtgatg attgtcggga tcgcattgcc gttttcaccg ctggccagtt
    20941 atctgcaatt acaggcgttg ccgttaagct atttcccgtg gctggttgcg attctggcag
    21001 ggtatatgac gttaacccag ttggtgaaag gattttatag ccgtcgttac ggctggcagt
    21061 aaagaataaa ctgggcatgg tggcccggat tcagattgca gacaaagtga gttttgttca
    21121 tgccggatgc ggcgtaaacg cctaatccgg cctacaaatt caatcaattg caggaatcac
    21181 gtaggcctga taagcgtagc gcatcaggcg attttgcgtt tgtcatcaga cttaatccgg
    21241 gcatgatagc ccggatttcc atcaagatta gcgacgaata gcgatcgctt cgatctcaat
    21301 ctttacgtct ttcggcagac gggcaacttc aacgcaagaa cgtgccggga aggtggcgtt
    21361 gtgttcggtg aagaaggttt cgtaagtggc gtttacggtt gcgaagtcgt tcagatcttt
    21421 tacaaacacg gtagttttaa cgatgtcgcc cactttcaga ccagcggctt cgacgatcgc
    21481 ttttacgtta tccagcgact gacgtgcctg tgcagcgacg tctgccggta cttcgcccgt
    21541 tttcggattt accgggatct gaccggaggt gatgatcata tttcccagat caacgccctg
    21601 tacgtaagga ccgatagctg ctggtgcatt ttccgtcgcg atagttttgc tcatgatttc
    21661 tcctttatta cagcggtaaa aagtctggct cattataggg agccagactt ttattaccaa
    21721 ccgcaattaa ttggccagca ccacattatg ggaaaactct ttttcacagt atttgcattt
    21781 gagcgcgatg tcattggcgc gttttcgcac ggcaaagctg gatgaaaccg gttcggcatg
    21841 gctgatacag ttgctgttcg ggcagaccaa cacattgtcg atgcgctctg gcagactggg
    21901 gcgcgattta cccaccactt catagttgtc gatacggtta accgtggctt gcggcgcata
    21961 caatgccagt tgatctacct gatcttcact caaaaaggta ttttcgattt tgatcagatc
    22021 tttgcggccc atctcgccag aaggcaggtt cagaccaatg gtgatgcgct gatccgtttc
    22081 ggtcagcttg aacagactca acagcttaaa accgatctgg gcggggatat ggtcaattac
    22141 cgtgccgcgt ttaatagctt caacctgcaa tttattatcg tgtgtcatct ctatttcccc
    22201 ttacagtacc agatcgcgat tcagaaccag tgccagtaac gcctggcgag cgaaaatccc
    22261 gttgcctgcc tgctggaagt accaggcgtg tggcgtttta tcaacatccg tcgcaatttc
    22321 atcaacacgc ggcagcggat gcagtacttt catattggct ttggcgttgt ggagatcgct
    22381 ggcgcgaaga acaaactgcg ctttcacgtt ggcgtattcg gatgggtcca ggcgctcttt
    22441 ttgcacgcgg gtcatataca ggatgtctac ttccgccatc acttcttcaa tagagctgtg
    22501 cagactccat gcgatccctt tttcatcgag catatccaga atgtattgcg gcattgccag
    22561 cgcgtccggc gcgatgaagt aaaaacggtt gccgtcgaac ttcgctaacg cctgagtcag
    22621 ggagtgaacg gtgcggccat atttcaggtc accaaccatt gcgacgtgga gattgtccag
    22681 acgcccctgg gtttcctgaa tagtgaataa gtccagcaag gtttgcgtcg gatgttggtt
    22741 ggagccatca ccggcattca gtaccggtac attgccggaa aactcggtgg ccaggcgcgc
    22801 cgcaccttcc tgcggatgac gcatcactat cgcatcgacg taagtgctga taaccgaaat
    22861 ggtatcggcc agcgtttcgc cctttttacc cagtgatgta ttggcgctgt cggagaagcc
    22921 caccacgctg gcacccaggc ggtgcatgga agtttcgaaa gagaggcggg tacgggtaga
    22981 ggcttcgaag aagcagctgg caatgacttt gtgcttcaac agctctggtt gcgggtttgc
    23041 tttcagtttt gccgctgttg ccagcaccag attaaggtca tcgcgactaa ggtcgtttat
    23101 ggaaatgata tgtttctgat atagcggatt agccatcttt tatctcctga cgcctgggca
    23161 aaaaaaagcc cctcgattga ggggctggga atgggtgatc aacgggaaga aaaacggcag
    23221 gccagcgtct tttttcagac gcggtaagac aaaatgtcga acacactgaa ccatacatcc
    23281 tcccggcaaa ttgtccggca ttatactcat cgtcagaagc ggtgcaagca attgatgcaa
    23341 tattttgtca gcgcaaacgg tttatttgaa tttaaagtta aaatatatgc atttttattt
    23401 actgtgattc tggggggaac tgtcgttcgg tatcaataat tgcagacatt tcctgctttt
    23461 ttctttttca ctttcacgca atcagatatg cattttattc cattcatccg acttataggg
    23521 cggagtgtga aaacgaacgg ctagcactat tgcttagagc tcagggatgc gcgctatcac
    23581 tttaatttca aaatcaaagc ctgccagcca tgtaacgccc accgccgtcc aggttggata
    23641 agatggcgcg ctaaatattt catttttcac cgtcatgatg tcttcaaatt gtttttctgg
    23701 atcggtatga aagctcgtaa catcaatgat atcgtcaaaa gtgcatcccg cagctgccag
    23761 ggtcgcatgc aaattatcaa aggccagtct gacttgttgc tgaaaatcgg gttctggtgt
    23821 tccgtcctct cgacttccta cttgcccgga aacaaacagc aaatcgccgg aacgaatagc
    23881 cgcagaataa cgatgctcag catacagtga atgtcgtcca gcagggaaaa cagcggttct
    23941 ttctaccatt tggttatcct caagatttac gacatgaaca gaagatttat atttaccagg
    24001 agccgctttt agcggacgac gtgagtaaac aaaacccaga catcatggga aatggctggg
    24061 cttaattgag cgtagtcggt tatgcgccaa acgcgccatc aatggtatgc atcgcgccgg
    24121 taacaaaact ggcttctggc cctgctaacc atgcgaccat accagcgacc tcttccggtt
    24181 gcccatgtct tttgatggcc ataaaactat gcgacatatc gcgcattggc ccgttggcgg
    24241 gattagcgtc ggtatcaatt ggccctggct ggacgacgtt gatggtgatc ccacgcggtc
    24301 cgaaatcacg ggccagcccg cgcgccatgc cttgcagggc agatttactg gcggcataag
    24361 cagccatgcc tgcaacaggc atacgatcgc cattcacgga gccgatgatt aagatgcgtc
    24421 cgccttcggg catctgccgg gcggcttcaa cagaggcatg gtaaggagca tgaatattga
    24481 ttttgaaaag gcgatcaata tcgtcggcat ttaattccag ggcatcgcca aagacgccaa
    24541 tacctgcatt taccaccagg atatccaatg cgccgctctt acgaacgaca tcaataacag
    24601 cgtctttgtc agcactatct gtgaatactg ctgtcgctcc agtctcttgt gccaggcgtt
    24661 cagcggcatc tttcgacccc gcataggtga atcgtacatt ggccccatcg gtgacgaaac
    24721 gacgtacgat agcggcaccg attccacggc taccaccgag gatgagaact gtcttacctg
    24781 taaaagcgcc cataaggact ccttgattta ttatgtaaca tgcattacaa aactgtttta
    24841 actttctgtc aacaagtttt gtaatggtca ctaaaaaaca atctcgcgtt ccaggtcgtc
    24901 ccagacgttt cgctcctgag caggcagtct ctgcggcaaa agtgcttttt caccaaaaag
    24961 gtttcgatgc tgtcagtgtt gctgaagtta ctgattatct tggtattaac cccccgagcc
    25021 tctacgcggc ttttggcaat aaggctgggt tatttagccg tgtactcaat gaatatgtcg
    25081 gtacggaagc tattccgctt gccgatattc ttcgtgatga tcgtccggta ggggagtgcc
    25141 tggctgaggt attaaaagaa gcggcgcgcc gatatagcca aaacggcggc tgcgctggct
    25201 gtatggttct tgaaggcatt catagtcatg atccacaagc gcgtgatatt gccgttcaat
    25261 attatcacgc cgcagaaacg accatttatg actatatcgc caggcggcat ccacaaagcg
    25321 cacaatgtgt gactgatttt atgagtaccg tgatgtcagg gctttctgcg aaggcacggg
    25381 aggggcactc catcgaacaa ctctgtgcaa cagctgcact ggcgggggaa gcgataaaaa
    25441 ctattctcaa ggagtgatgc gggccttgac ccgaaaggcg ggaacgcgcc tgccgataag
    25501 ttgtgataag acaataattc acgcgttaag gctagcggaa ttgatcatct tttcgtataa
    25561 cgatggaaat gaaacgttgt tttaattaag gagtggaaaa gatgatcatc ggaaatattc
    25621 ataatcttca gccgtggcta ccccaggagt tacgccaggc gattgagcat atcaaagcac
    25681 acgttacggc agaaacgcca aagggcaagc acgatatcga aggcaatcgt ctgttttatc
    25741 ttatctcgga agatatgacc gagccgcacg aagctcgccg tgcggagtac catgcccgct
    25801 atctcgacat tcagattgtg ttaaaaggtc aggaaggcat gaccttcagc acgcaacctg
    25861 caggcacccc ggataccgac tggttagctg ataaagacat cgcatttttg ccggaaggcg
    25921 ttgatgagaa aacagttatc cttaatgaag gtgattttgt tgtgttttat ccgggggaag
    25981 tgcataaacc gctgtgcgca gtgggcgcgc cagcccgggt tcgcaaagca gtagtgaaga
    26041 tgctgatggc gtgatgactt ttcgccgtaa ataactccag gtttacggcg agtttgtgaa
    26101 aagagcgttt tttgatattt ttttgtgagt aaaatttgta atgcttagac gttcttatta
    26161 actcaaggag ttcgtcatga gcaaaatatc agatttgaat tattctcaac acattacatt
    26221 agccgacaat tttaaacaaa aaagtgaagt tttaaatacc tggcgtgttg gaatgaataa
    26281 ttttgcccgt aatgccgagg ggcaggataa cacaagaaat atccttgatc ctaagacatt
    26341 tttggaattt ttggtaaaaa tatttactct aggttatgtg gattttagca aacgctccaa
    26401 cgaagcggga agaaatatga tggctcatat tgagtcctca tcttatatca aaaataatga
    26461 tggcagtgag ataataaagt ttgttatgaa taatcctgaa ggggaacgag cggatttatc
    26521 taaggtggaa atagaaatca cactttccac tttcactact atggggactc gtcaaggaca
    26581 tacggctatt atatttcaac aacctgatgg ttcgactaat cgttatgaag gaaagtcctt
    26641 tgaaagaaaa gatgagagtt cattacacct gattactaac aagattctgg catgttacca
    26701 acgcgaagct aacaaggaaa tagctcgtct attaaatatt tcacaggagt taaataattc
    26761 tcaggacttg aataactcgc aggtgagttg taaagattca gttgattcta cgattacgga
    26821 tttattagaa aaaccattga ataatgcatt attagcaata aggaaagaac atctgctatt
    26881 aatgccttat gtatgtaatg aatcgatttc atacttgctt ggtgaaaaag ggatactgaa
    26941 agaaatagat gaccttaacg cagtaaataa ttatcttctt aataataaga aagctactga
    27001 caatgaaatt aatgacatca aagttaacct gtctcatatt cttattgatt ccttagatga
    27061 tgcaaaagtt aaccttacac cggtcatcga ttctggagac ttttttaaaa tccccatata
    27121 ttaatgatgt aagaatactg gattggtgtt ttaataaaag aatgcaatat tttg
//
LOCUS       DAJTYD010000038        26307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTYD010000038 DAJTYD010000000
VERSION     DAJTYD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.038"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            559..1971
                     /gene="pykF"
                     /locus_tag="OO585_001466"
     CDS             559..1971
                     /gene="pykF"
                     /locus_tag="OO585_001466"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6629100.1"
                     /translation="MKKTKIVCTIGPKTESEEILAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2282..2518
                     /gene="lpp"
                     /locus_tag="OO585_001467"
     CDS             2282..2518
                     /gene="lpp"
                     /locus_tag="OO585_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6629101.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(2582..3586)
                     /gene="ldtE"
                     /locus_tag="OO585_001468"
     CDS             complement(2582..3586)
                     /gene="ldtE"
                     /locus_tag="OO585_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6629102.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(3735..4151)
                     /gene="sufE"
                     /locus_tag="OO585_001469"
     CDS             complement(3735..4151)
                     /gene="sufE"
                     /locus_tag="OO585_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6629103.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4164..5384)
                     /gene="sufS"
                     /locus_tag="OO585_001470"
     CDS             complement(4164..5384)
                     /gene="sufS"
                     /locus_tag="OO585_001470"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6629104.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(5381..6652)
                     /gene="sufD"
                     /locus_tag="OO585_001471"
     CDS             complement(5381..6652)
                     /gene="sufD"
                     /locus_tag="OO585_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6629105.1"
                     /translation="MAGLPNSSNALQQWHHLFDAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(6627..7373)
                     /gene="sufC"
                     /locus_tag="OO585_001472"
     CDS             complement(6627..7373)
                     /gene="sufC"
                     /locus_tag="OO585_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6629106.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7383..8870)
                     /gene="sufB"
                     /locus_tag="OO585_001473"
     CDS             complement(7383..8870)
                     /gene="sufB"
                     /locus_tag="OO585_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6629107.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(8879..9247)
                     /gene="sufA"
                     /locus_tag="OO585_001474"
     CDS             complement(8879..9247)
                     /gene="sufA"
                     /locus_tag="OO585_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6629108.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(9794..9982)
                     /locus_tag="OO585_001475"
     CDS             complement(9794..9982)
                     /locus_tag="OO585_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6629109.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10082..10492)
                     /gene="menI"
                     /locus_tag="OO585_001476"
     CDS             complement(10082..10492)
                     /gene="menI"
                     /locus_tag="OO585_001476"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6629110.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10489..13545)
                     /locus_tag="OO585_001477"
     CDS             complement(10489..13545)
                     /locus_tag="OO585_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6629111.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13934..15046
                     /gene="ydiK"
                     /locus_tag="OO585_001478"
     CDS             13934..15046
                     /gene="ydiK"
                     /locus_tag="OO585_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6629112.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15232..15339
                     /gene="rprA"
                     /locus_tag="OO585_001479"
     ncRNA           15232..15339
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO585_001479"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15475..15854
                     /locus_tag="OO585_001480"
                     /pseudo
     CDS             15475..15854
                     /locus_tag="OO585_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            15799..16521
                     /locus_tag="OO585_001481"
                     /pseudo
     CDS             15799..16521
                     /locus_tag="OO585_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16533..17399
                     /gene="ydiB"
                     /locus_tag="OO585_001482"
                     /pseudo
     CDS             16533..17399
                     /gene="ydiB"
                     /locus_tag="OO585_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17431..18189
                     /gene="aroD"
                     /locus_tag="OO585_001483"
     CDS             17431..18189
                     /gene="aroD"
                     /locus_tag="OO585_001483"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6629113.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18335..19930
                     /locus_tag="OO585_001484"
     CDS             18335..19930
                     /locus_tag="OO585_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCR6629114.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            19944..21095
                     /locus_tag="OO585_001485"
     CDS             19944..21095
                     /locus_tag="OO585_001485"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR6629115.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21138..22049)
                     /locus_tag="OO585_001486"
     CDS             complement(21138..22049)
                     /locus_tag="OO585_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6629116.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22363..23134
                     /locus_tag="OO585_001487"
                     /pseudo
     CDS             22363..23134
                     /locus_tag="OO585_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23154..24092
                     /locus_tag="OO585_001488"
     CDS             23154..24092
                     /locus_tag="OO585_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6629117.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLVATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24148..25437
                     /locus_tag="OO585_001489"
     CDS             24148..25437
                     /locus_tag="OO585_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6629118.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25434..25727
                     /locus_tag="OO585_001490"
     CDS             25434..25727
                     /locus_tag="OO585_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6629119.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            25784..>26307
                     /locus_tag="OO585_001491"
     CDS             25784..>26307
                     /locus_tag="OO585_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HCR6629120.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIA"
BASE COUNT         6564 a         6679 c         6478 g         6586 t
ORIGIN      
        1 attctttata cctatttatc atgccaacta tcagcatata tcaatctaac caattaaaca
       61 aaaatcaaac aaaatcagac aaataacgcg ataaattatt ttaactgtag caattgagcg
      121 atgatatatt tatacaccgg atgaactttc acttatcctc acactgacaa ctttggcacc
      181 agacgttgcg caaacagcga agtttttgcg taaccttttc cctggaacgt taaatctttg
      241 ataacaattt attgtctaac aagttgtata ttttttgaaa cgctgttttt gttttccttt
      301 tggattaatt tcagcgtata atgcgcgcca attgactctt gaatggtttc agcactttgg
      361 actgtagaac tcaacgactc aaaaacaggc actcacgttg ggctgagaca caagcacaca
      421 ttcctctgca cgctttttcg atgtcaccta tccttagagc gaggcaccac cactttcgta
      481 ataccggatt cgctttccgg cagtgcgccc agaaagcaag tttctcccat ccttctcaac
      541 ttaaagacta agactgtcat gaaaaagacc aaaattgttt gcaccatcgg accgaaaacc
      601 gaatctgaag agatattagc taaaatgctg gacgctggca tgaacgttat gcgtctgaac
      661 ttctctcatg gtgactatgc agaacacggt cagcgcattc agaatctgcg caacgtgatg
      721 agcaaaactg gtaaaaccgc cgctatcctg cttgatacca aaggtccgga aatccgcacc
      781 atgaaactgg aaggcggtaa cgacgtttct ctgaaagctg gtcagacctt tactttcacc
      841 actgataaat ctgttatcgg caacagcgaa atggttgcgg taacgtatga aggtttcact
      901 actgacctgt cagttggcaa caccgtactg gttgacgatg gtctgatcgg tatggaagtt
      961 accgccattg aaggtaacaa agttatctgt aaagtgctga acaacggtga cctgggcgaa
     1021 aacaaaggtg tgaacctgcc tggcgtttcc attgctctgc cagcactggc tgaaaaagac
     1081 aaacaggacc tgatctttgg ttgcgaacaa ggcgtagact ttgttgctgc ttcctttatt
     1141 cgtaagcgtt ctgacgttat cgaaatccgt gagcacctga aagcgcacgg cggcgaaaac
     1201 atccacatca tctccaaaat cgaaaaccag gaaggcctca acaacttcga cgaaatcctc
     1261 gaagcctctg acggcatcat ggttgcgcgt ggcgacctgg gtgtagaaat cccggtagaa
     1321 gaagttatct tcgcccagaa gatgatgatc gaaaaatgta tccgtgcacg taaagtcgtt
     1381 atcactgcga cccagatgct ggattccatg atcaaaaacc cacgcccgac tcgcgcagaa
     1441 gccggtgacg ttgcaaacgc catcctcgac ggtactgatg cagtgatgct gtctggtgaa
     1501 tccgcaaaag gtaaataccc gctggaagcg gtttctatca tggcgaccat ctgcgaacgt
     1561 accgaccgcg tgatgaacag ccgtctcgag ttcaacaatg acaaccgtaa actgcgcatt
     1621 accgaagcgg tatgccgtgg tgccgttgaa actgctgaaa aactggatgc tccgctgatc
     1681 gtggttgcta ctcagggcgg taaatctgct cgcgcagtac gtaaatactt cccggatgcc
     1741 accatcctgg cactgaccac caacgaaaaa acggctcatc agttggtact gagcaaaggc
     1801 gttgtgccgc agctggttaa agagatcact tctactgatg atttctaccg tctgggtaaa
     1861 gaactggctc tgcagagcgg tctggcacac aaaggtgacg ttgtagttat ggtttctggt
     1921 gcactggtac cgagcggcac tactaacacc gcatctgttc acgtcctgta atattgcttt
     1981 tgtgaattaa tttgtatatc gaagcgccct gatgggcgct ttttttattt aatcgataac
     2041 cagaagcaat aaaaaatcaa atcggatttc actatataat ctcactttat ctaagatgaa
     2101 tccgatggaa gcatcctgtt ttctctcaat ttttttatct aaaacccagc gttcgatgct
     2161 tctttgagcg aacgatcaaa aataagtgcc ttcccatcaa aaaaatattc tcaacataaa
     2221 aaactttgtg taatacttgt aacgctacat ggagattaac tcaatctaga gggtattaat
     2281 aatgaaagct actaaactgg tactgggcgc ggtaatcctg ggttctactc tgctggcagg
     2341 ttgctccagc aacgctaaaa tcgatcagct gtcttctgac gttcagactc tgaacgctaa
     2401 agttgaccag ctgagcaacg acgtgaacgc aatgcgttcc gacgttcagg ctgctaaaga
     2461 tgacgcagct cgtgctaacc agcgtctgga caacatggct actaaatacc gcaagtaata
     2521 gtacctgtga agtgaaaaat ggcgcacatt gtgcgccatt ttttttgtct gctatttacc
     2581 actactgcct cgcgcgtaac atattccctt gctctggttc accattctgc gctgactcta
     2641 ctgaaggcgc attgctggct gcgggagttg ctccactgct caccgaaacc ggataccctg
     2701 cccgacgata caacgcttta tcgactaact tctgatctac agccttattg tctttaaatt
     2761 gcgtaaagcc tgctggcagt gtgtatggca ttgtctgaac gttctgctgt tcttctgccg
     2821 atagtggtcg atgtacttca acataacgca tcccgttagg ctccacggaa tatttcaccg
     2881 gttcgttgat cactttcacc ggcgttcccg tccgcacgct ggagaataag gctttaatat
     2941 ccggtgcatt catgcgaata cagcccgaac tgacgcgtaa accgacgctg tccggcgcac
     3001 tggtaccatg aatgaggtat tcgccattac catgcgcgaa gcgcagtgcg taacgtccta
     3061 gcgggttatt tggtccggca ggaacgactg gcagtaattt aatgccacgc tccagcgaac
     3121 gttgacgaat gcctgccgta ggcgtccagg ttggattagg gattttctgc cccacacgcg
     3181 tttccatcac cggcgtttcc agcccctgca agccaatacc tatcggataa acctgcacga
     3241 tattttctcc cggcggataa taataaaggc gcagctctgc aaggttaacg ataatcccct
     3301 gacgcggtgc atcaggtaat aacagttgtg aaggaatagt tatcgtcgta ccaggttttg
     3361 gcaccggggc gatagtgtta ttggcttcaa ggatcaacat tgccgcagta tcaaaacgtc
     3421 gggcaatagc ctgaaggttt ttatcccctt cttgcaccgt atacgtttga ttttgcccaa
     3481 ccagtcggct tccggttggt ggtagcggat aatcaaccgc ccaggcagcc tggatggcgc
     3541 taaaagcgcc gataagcgtg agtgtaagca aagacgcgcg tttcattgta aacctcctgt
     3601 atttgccgga gactcacgct gaaacgtcgg atggcgctta tgttcacctg aaaccaaaac
     3661 actcctgtgc aggtcagtgt aaacattgac catccggcaa tgtgagccaa ccggatgaaa
     3721 gctgtccctt tagtttagct aagtgcagcg gctttggcgc gaattgcgcg aatcatcgct
     3781 tccagacctt gtgaacgaga tggggtgaga tgttgggtga gcgccatttt ttcaaaccac
     3841 ggacgcacat cgaaattgac aatatcctgc ggcgtcatct gatcgtagag aataaatacg
     3901 accgcaataa gccctttcac aatcgccgca tcgctgtcgc cctgcaattc aataattccc
     3961 tgcgcattct ggcgcatgac aatccacacc tgattctggc agccctgaat gctattttgt
     4021 gggcttcggt cttcgtcgcg taattctggc agacgctggc ccagctcaat aatgtagaga
     4081 tatttctctt cccagttggc gcagcgtaaa aaattacgca gcaatttttc tttatccggc
     4141 aataaagcca tagtgcctcc ctgttatccc agcagacggt gaatacgttg caggccggtc
     4201 accagacgat ccacttcttc atgagtgtta tacatggcca gcgacgcccg acacatcgca
     4261 gggacgttgt aataggccat taatggcata gcgcagtgat gtccggtacg cacagcaatg
     4321 ccgtaattat cgagaaaact gccaacatca taggcgtggt gtttaccgag attaaaagca
     4381 ataacgccaa gcctgttttg tgggccatag agagtgagat tcggtacaga ttccagctgt
     4441 gatagcgcgt aatgcatcag attcagttca tactcggcta tgttattaag ccccagcacc
     4501 gaaacatatt ccagcgccgc gccaagacca atgatgcccc cggtattggg tgtaccggct
     4561 tcaaaccgcc atggtgcttt ggtccaggta gtgccttcac tcaggctgac ggtggcgatc
     4621 atagaaccgc ccccttccca cggcggcatc tcctgcaaca aggcttcttt gacataaaga
     4681 atgccaatcc cggtggggcc atacagttta tgcccggaaa acacgtaaaa atcgcaatcc
     4741 agcgcctgaa catccaccgg atgatgcatc accgcctgag cgccatccac cagcactttt
     4801 gcgccatgct ggtgcgcaag cgtgatcatt cccgccagtg gattttctgt gccaagcacg
     4861 ttggagacat gagtaattgc cagcaggcga gttttctcat caaacagcgt atgcagcgtc
     4921 tccagttgca gcgtaccgtc ggggttgaga gggatcacac gcagctctgc gccaacgcgt
     4981 gcgcaaagca tctgccaggg gacaatgtta gcgtggtgct ccatctgact gatgatgatg
     5041 ttatcgcccg cccgcacatt gctgttgccc cagctattgg cgaccagatt gatcccttcc
     5101 gtcgtgccgc ggacgaacac cagctcttcc gccgaacggg cattaataaa cagcgatgcc
     5161 cgcttgcgca cgttctccat tttctcggtc gcttgggcgc ttaaggtatg aataccgcga
     5221 tgcaccgccg cgtagccatg acgataaaac tcggcctcgg cgtcaatcac ctggctcggt
     5281 ttctgtgcac tggcggcgct gtcgagataa gccagcggca aaccgtttac ctcacgcgaa
     5341 agcaccggaa agtcggcccg cactttgtcg acggaaaaag tcatcttgca cctcctggca
     5401 gccgttgacc gattcgggcc agcacctgct gtttaagccc ctcatcacgc agtgcttccg
     5461 tcagttcggc agcaaaggcg taaatgatca tctgctgggc atcctgctga ttgatcccgc
     5521 gcgagcgcag atagaacatc tgttcatcat caatacgccc caccgtcgcg ccgtggctgc
     5581 atttcacatc atctgcatag atttccagct gcggtttcgt atccacttcc gccagtttgc
     5641 ccatcagcag attattgttg gtcatctgac catccgtttt gatggcgtgc tgcgcgacgt
     5701 tgatcaaacc gttaaatacc gcgcggcctt tgtcgctgac gatagttttg tgcaactgtc
     5761 ggctgttaca aaaaccttta ttgtgttcca gccaggtacg ggtatcacac acctcgtttt
     5821 tcaccggcat cgccaggcta ttgatccgca gcgtgctgtt ttcgccattg agttgcgtac
     5881 tggtgttgtg tcgtaacact gcgccaccca gcaggaaact atggctaaat gcggtggcat
     5941 cctcagccag caacaaatcg ttatgagcaa agtggtgact gagcgggttt tcaaacgcca
     6001 gcttgatatg ctgcaagtgg gcgttcgctg cgacattgat agtgaaccgt gccccggtaa
     6061 aatgacgagc atcattaagg ctgacaaaat gttcgatcac cgttgcttcg gcaccttccg
     6121 ccagatccag atgatgccgg taatgggcag tgttcacctc ttcacctgcc acgccctggg
     6181 tgatatacat taacagcaat ggctttgccg gccgttgacc gcgcttcacg gcgatatgcg
     6241 tcacgctttg tgccaggctt tccgtcaaat gcagaaacac ttccgcctga atagcgtcgg
     6301 gtaacccctg acggtcgtcg ttaatgctca cttcatatcc gctgccttca attgcatcgc
     6361 tcagcgcggg cacgtaacgt ccatcgacaa acaccagccg cacggcgtct aacgttaacg
     6421 ctaaggcatc acgctgctgt ggggatatct cccccgcaat gctgacaaac tggctattgg
     6481 tcagcccttc cagcgacgta tatttccagt tttcatgttt acgtgtcggc agtccggtac
     6541 gcagcaattg ttgtaaatgc tgctgtgctt gcggggagcg ttttgtccct tcagcgtcaa
     6601 acaagtgatg ccactgttgc agcgcgttac tgctgttcgg taagccagcc ataaccctgc
     6661 tcctccagtt gtttgaccaa cgtgaaatcg ccggatttca caattcgtcc ctgatatagc
     6721 acatggacgt aatcaggctt gatgtagtcg agaatgcgtt ggtagtgcgt aacaatgatg
     6781 aatgagcgct tgccatcacg cagggagttc acgccatcgg cgaccacttt taatgcgtca
     6841 atatccagcc cggagtccga ctcatcaaga atgcataact ccggttccag caccgccatt
     6901 tgcaaaatat cgttgcgctt tttctcgccg ccggaaaaac caacgtttac cgaacgggtt
     6961 aataaatctt ccggcatctt caggagagcg attttctctt ccatcaaatc ctgaaaatca
     7021 aagcggtcga gcgtttcctg gccgcgatag ctgcgcactg cattaagtgc cgtttgcagg
     7081 aaaaactggt tactgacgcc tggaatctcc accggatact ggaaggccat aaagatgcct
     7141 tcgcctgcac gatcttccgg cgacagtgca agcaaatctt tgcctttgaa ctcaaccgtg
     7201 ccgcccgtca cttcataatc ttcgcgcccg gcaagcgttg ccgataaggt acttttgccc
     7261 gaaccgtttg gccccataat ggcgtgaact tcgccgggac gaacggcgag gcttaatcca
     7321 cgcaggatag ctttatcttc cacgctgacg tgtaaatctt taatacttaa catgtttatt
     7381 ccttatccga cgctgtgttc aagactgatc gcgaggagtt tttgtgcttc aacggcaaat
     7441 tccagcggca gttctgagaa cacgtctttg cagaaaccgt taacaatcat cgagatggcg
     7501 tcttcttcgc tgatcccgcg ttgcaggcag taaaacagtt gatcttcacc aatacgtgat
     7561 gtcgttgcct cgtgctccag ttgtgcgctg ttattgcgac attcgacata cgggaaggta
     7621 tgcgccccac aattagcgcc aatcagcatt gagtcgcact gagtgaaatt gcgcgcattg
     7681 gttgccgtcg gcatgatttt cactaagccg cgataactgt tctgactatg tccggcagag
     7741 atccctttcg agataatggt cgatttggtg tttttaccga tgtggatcat cttggtgccg
     7801 gtatccgctt gctgatgacc gctggtcagc gccactgagt aaaactcacc aatggagtta
     7861 tcgccgcgca aaatgcagct gggatatttc cacgtaatcg cggacccggt ttctgattgc
     7921 gtccatgaca ttttgctgtt ttcgccttcg cacaaagcac gcttggtgac gaagttgaga
     7981 ataccgccgg tgttgttatc gccagggaac cagttttgta ccgtggaata tttcacctcg
     8041 gcgtttttat ggatgatgac ttccaccact gccgcgtgta actgatagct gtcacgcacc
     8101 ggagcggaac agccttcaat gtagctgacg tagctgtctt cgtcggccac cagaatggtg
     8161 cgctcaaact gcccggtttt ttccgcgtta atgcgaaaat aggtggaaag ttccatcggg
     8221 cagcgcacgc ctttaggcac ataaataaac gtaccgtcag aggctaccgc cgcattaagc
     8281 gcggcaaaga agttgtcatt ccccggcacc acggtgccga gatatttacg caccagttcc
     8341 gggtgatcgt ggatcgcctc accaaaggaa cagaaaataa ttccctgctc cgccagtttt
     8401 tcgcgataag tagtggcaac cgaaacagag tcgaaaatgg catccaccgc cacctctttg
     8461 ccttcccgca cgggaacgcc caactgctca aacgccgcct ccacctcttt acttaaaaag
     8521 gcgttcgcgc cagtttgctg caccgcgcca ggttcagacg cgcaagtgtc gtcacaatta
     8581 ccgcacgatg gtgctgagta gtagctgtaa tcctgataat tcagcttgtc gtagtgcgct
     8641 ttcaaccagt gcggttcttc catctccagc catgcgcgat aggcgtttag acgaaactcc
     8701 agcatccact ccggctcatt acgcttcgcc gaaattgcgc gcaccacctc ttcgtttatc
     8761 ccctttgcca gctcatcggt ggctaactgg gtgaagaatc cttctttata attcagcggg
     8821 ccgccggtcc aggttttgac atcgtcagtt gcttcagtat tacgagacat agtaccgcct
     8881 ataccccaaa gctttcgcca cagccacatt cattctgggc tttagggttg tgaaatttga
     8941 atatctgatt aagtccttca cgaacgaaat cgacttccgt gccatcaata aacggcatcg
     9001 cttgcagcgg gacaaacagc ttcgcgccgt cgtgttcaaa cagcagatcg tctttgtccg
     9061 gctcgctaac actgtcgagc acatagccaa agcccgcgca gcccgtttgc ttcacgccta
     9121 aacgcacgcc gaccataccc ggctgctttg ccaccagctc acggatgtgt atcgccgctg
     9181 cgggtgtcag cgttaagcct tgccaggcga aatcttgtgg gttaaaggtt cctgaatgca
     9241 tgtccatcga tttacctcac ttcatcgctt tcagcgtata acagcatgtt ggtgataatg
     9301 attatcagtt caacccagca aacgcagggg ctttaccgta aaacatgctt tttgcctgct
     9361 tttaataagc atagaccctg atgtgtgggt taacaggcac gctaagaatt aggtatctca
     9421 ttgttagata atggttatta tctaaggagc attaaggctg tgatgaaaaa gaaaaagttg
     9481 tattaaaatg actatttaag agataggtaa aaaagtgcag cgttcagaaa taagaaaacc
     9541 cttaagtctg tgcgacacag gcttaagggt ttctacccca tccggcgctt atctccggca
     9601 ctctcagtgg cttagctctt gaaggggcga taagaataat ctcataaagc taacccgccg
     9661 ttttaacaca aactgcgatt agtattattt ttgaacaata tcaggcggta gataagcagt
     9721 attaagaagg tcatcgaacc tggacggagg ttaatccagg tcgatttggc gaacttgcgg
     9781 cattaagtca ggatcaatgc acgcccagac gccaggcaaa gtagatttct tcttttaatt
     9841 cagcagaaga gagagtaagc aggtcagcaa attcaagttc tagttgtttc agacgtttga
     9901 gatattgggc aggtgaaaga ttgctctggt cacggcgtaa aaattcaatg gccagctggg
     9961 tgggatcaag ttgagtagac atagcatcct cgcttttaga caagacgtgt acagtatacc
    10021 accgtttact gtgcagataa tgaccaaaag caatatgcgt cacacttttc tggtgacaac
    10081 gtcacaaaat ggcggtcgtc aatcgtgacg aacagcacaa acgccctttc tcatcgaaga
    10141 tttcaatctg ccagacctgg tgacgcgaac cgagatgcaa cggtttacat atgccgcgca
    10201 cccgcccttc tcgtgccgag cggacgtgat tagcattgat ttccagacca accacttttt
    10261 gctcaccttc ggtacataaa taaccggcaa cggaaccgat actttcggcc agtaccacgg
    10321 atgctcctcc atgcagcaac ccgaaaggct gctttgtccg cgagtctact ggcattgtcg
    10381 cttcaagggt gtcatcacca atatgttcaa agcgaatatc caggaacccc accatgtttc
    10441 cttcacccat agcattcagt gcttccaggg tgattttccg tttccatatc atttaataat
    10501 ctccagtaaa gcctgcacag gatggcgtac ccccgtgcct tcaacccgtt ttacctggct
    10561 acggcaggaa tatccggtcg ccagacagcg gttacgcggc agtcgctgca tagcctgatg
    10621 ccaggataac tcatagatcc cgagcgaatt ttcatggttt ttcgcttcat gtccgtaagt
    10681 ccctgccatg ccgcagcaac ccacgctgac attttccagt ttcgcgccaa aacgggcaaa
    10741 tatcgcggcc cattgtgctg gcgcacccgg caaggcggta acttcggtac agtgaccaaa
    10801 gaaataccat gattcaccgc tgactgtagc cacaggctgt gactcaagtg cgcttgccag
    10861 ccattcattc gccagtaaga cgttaaactc gccacgctcc tcgcccagcg ccagtttata
    10921 ttcatcgcga taacaaagta ccagcgccgg atcgacgccc accattggca tacccagctt
    10981 cgccatacgg ttgaggaaat ccgccgtctt tttcgccgtc ttcgcaaaac gattaagaaa
    11041 acctttaata tgctgggctt tgccatttgg cgaaaatggc agtaacacag gctggaaacc
    11101 taatttttcg accagacgga caaaatccgc caccacttgc gcatcgtaat agctggtaaa
    11161 ggggtcctgc accaccaaca ctgtgcgcgc tttctgctct gcattgagag attcaagctg
    11221 ttccagcgtc atgtttgccg agcgatgccc caccatttgt tgttgtagcg aggggaccga
    11281 cagcagcggc aaatcaacca tgccgatatg tttttccgag agtttgcgca ccagcggctg
    11341 gttaatgaag aagttaaagg ttttcggcgc gcgtgccatc agcggcgcgt agctctcgac
    11401 cgtagcgacg aggtggtcgc gcagcgggcg taaataacgg gtgtgataga gctgcagaaa
    11461 acgagagcga aactccggca catcaatttt gatggggcac agggtcgaac acgctttaca
    11521 ggccagacag cccgacatcg cctctttgac ttcgtgtgag aagtcatatt cgcctttatt
    11581 cgcatgccag ctattgcgcg tgcgggcaat taacgtccgc aaactgacgc cggattcagg
    11641 cagttcttgt tccagtttga gtggatcaac gccgcgatcc gccaacaaac gcagccattc
    11701 acgcaccagc gttgcgcgcc ctttcggtga atgaatccgg ttctgggtga tcttcatcga
    11761 cggacacatc ggactacggg catcaaagtt gaagcataaa ccgttgccgt tacactccat
    11821 cgcaccgcgc cactgctggc gtaccgcaat ggggatctgc cgatcgaatg taccgcgctt
    11881 caccgcgtcc actttcatca tcggcgcatc gagaccttct ggcgggcaaa tcttccctgg
    11941 gttgagtcgg ttatgcgggt caaatgccgc tttcactttg cgcagttctg caaaaagttc
    12001 ctcaccgaaa aacgccgggc tgtattcagc gcgaaaacct ttgccgtgct cgccccacaa
    12061 caaaccaccg tatttcgcag tcagcgccac cacgtcatca gagatttgct tcatcaaaat
    12121 ctcttgttga ggatcgcaca tatccagctc tggacggacg tgcaagacac ctgcgtcgac
    12181 gtgaccgaac ataccgtagc ttaagccgtg gctgtcgagc agcgcgcgaa attcagcaat
    12241 ataatccgcc aggtgttccg gcggtacgca ggtatcctca gcaaacggaa ttggcttagc
    12301 ggcacctttg gcattgccaa gcagaccaac ggcttttttg cgcattgcat agatacgttc
    12361 aacccccgcc agctcacggc acacctgcca gccgatgaca cctgcttgat gactggcgat
    12421 cagctcatcc aaccgcgcac agagtgcatt tacccgctca tcaatcagcg cctcatcatc
    12481 accagcaaat tccacaatgt tcagcccgag catctcttgg tcaggcacat cggtaatcaa
    12541 ctcgctgacg gaatgccaga caatatcttc ccgcgccaga ttcagcactt ttgagtccac
    12601 cgtctctacc gaaagcgccc gcgcctcaac cataaacggc gcgttacgca gcgcagagtc
    12661 aaaagagtca tatttgacgt tcaccagacg gcgcacttta ggcaagcgcg taatatccag
    12721 ccgcgcttcg gtaataaagg ccagcgtccc ttctgaaccc gtcagaatgc gcgtcaggtc
    12781 gaactcggtc atctcatcgt taaagacatg acgcagatcg taaccggtaa gaaagcggtt
    12841 aagtttgggg aagttgtcga taattaactg gcgttgctga cggcaacgtt gataaaccgt
    12901 gttataaatt cgcccgattg tggtattgga tttacccagc gtttccgcca attcgacggg
    12961 taaaggttgc gtatcgagaa catcgccccc caacaacacc gcgcgtacgc caagtacgtg
    13021 atctgacgtt ttgccataga ccagcgatcc ctgaccggat gcatcggtat tgatcatccc
    13081 accgagcgtt gcccggttgc tggtcgaaag ttccggcgca aaaaagtagc cgaacggttt
    13141 caggtactga ttgagttgat cttttatcac cccggcctca acgcgcaccc agccctcttc
    13201 agggttaatt tcgatgatgc ggttcatatg gcgggacata tcaacaataa tcccctggtt
    13261 gagcgcctga ccgttagtgc cggtgccgcc gccgcggggg gtaaagatca gcgatgaata
    13321 gcgttcctgc gcggcaagac gggcgatcag cgccacatct gcggttgaac gcggaaatac
    13381 caccgcatcg gggagaagtt ggtaaatact gttgtcggtc gacattgtca gacgatcggc
    13441 ataacttgtc gccgtatcgc cggtaaaacc ttgttgctcc agctcttgca aaaaattaag
    13501 caccagttga acgacgccgg gtgcctgaga aatctgtgga atcattatat tgcccctttc
    13561 ctgcggtctg tgatgtaggt cgatacacta ttctttcagg cggctgcaat agcgcactga
    13621 aaggtgatgt ttatttactc tatggatttc gagttgcagg aaggcggcaa gcgagtgaac
    13681 tccaggagct tacaatagta agtgactggg gtgaacgaac gtagccgcag cacatgcaac
    13741 ttgaaatacg acgagtaaat cgtttgcgtg ttgcctgagt tgttgtacca catttttttc
    13801 taacacgccc atcagaatta agggcagaat cggcctgtta aaaaccgctg aaattgctca
    13861 tcattatgca ggtgagtttc gcgtgttcac gtcgcgtcga cgatttgacg cacaaaaaag
    13921 gtgaaaagta gttatggtaa atgttcgtca gcccagggat gtcgcacaaa ttctgctttc
    13981 ggtgctgttt ttagccatca tgattgtggc atgtctgtgg attgttcaac cctttattct
    14041 cggctttgca tgggccggta cggtggttat cgccacctgg ccggtattgt tacgtttgca
    14101 aaagatcatg tttggccgcc gctctctcgc cgttctggtg atgacgctgt tattagtgat
    14161 ggtgtttatc atccctattg ctttgctggt taacagtatc gtcgacggca gcggcccgct
    14221 aattaaagcc atttccagcg gcgacatgac gttacccgat ctggcgtggc ttaataccat
    14281 tccggtgatt ggcgcgaagc tgtatgcagg ctggcacaac ttgctggata tgggggggac
    14341 ggcgatcatg gcgaaagtcc gcccttatat tggcaccacc accacctggt tcgttgggca
    14401 ggcggcgcat atcgggcgct ttatggtgca ttgtgcgctg atgcttctct tcagtgccct
    14461 gctgtactgg cgcggtgaac aggtggcaca aggcattcgc cattttgcaa cccgtctggc
    14521 aggcgttcgc ggagatgccg ccgtactgct ggcggcacag gctatccgcg cggtggcgct
    14581 gggtgtggtg gtgacggcgt tagtacaggc agtgcttggc ggtatcggcc tcgccgtatc
    14641 cggcgtacct tatgcaacgt tgctaacggt gttaatgatc ctctcctgcc ttgtccagct
    14701 tggcccgttg ccggtactga ttccggcgat tatctggctc tactggactg gcgataccac
    14761 ctggggaacg gtattgttag tgtggagcgg tgtggttggc acgctggata acgtcatccg
    14821 cccaatgtta attcgcatgg gtgccgattt accgctgatc ctgatcctct ctggtgttat
    14881 tggtggtttg attgctttcg ggatgatcgg tctgtttatt ggtccggttc tgttagccgt
    14941 ttcctggcgt ctgtttgccg cgtgggtgga agaagtcccg ccgccgacgg accaaccgga
    15001 agaaattctc gaagaacttg gcgaaatcga gaaaccgaat aagtaatttc tcatcaggcg
    15061 gctctgccgc ctgattgtta acccccgcta attgatattt gcttgctctt ccccatattt
    15121 tcctgcttac cgccattcag ctggtagtac ctgtcgcaaa ttctttacag tttttaaact
    15181 aatgagacga atctgatcga cgcaaaaagt ccgtatgcct actattagct cacggttata
    15241 aatcaacata ttgatttata agcatggaaa tcccctgagt gaaacaacga attgctgtgt
    15301 gtagtctttg cccatctccc acgatgggct tttttttaac atttttccac ttcgctacct
    15361 cgcccctcac tctttccaat aatgttgcac aagatactgt gactctcttc aagccgtcag
    15421 caggctgata atgttattta tattttattg tttatttcat gcagccatta cacgatgaac
    15481 gcttatgaac tccaggcact ccgacatatt tttgccatga ctattgatga atgcgcacct
    15541 ggattgccca gaccggtgac agtgaaagct ggcgtcagtg ggaaaatggc aagtgtgcca
    15601 ttcctgatcg tgtagtcgag caactgttgg ctatgcgtca gcaaagaaaa aaacatcttc
    15661 atgccatcat agagaaaata aataaccgca tcggcaacaa caccatgcgt tttttccccg
    15721 acttaaccgc atttcaacga gtctaccctg atggcaattt tattgactgg aagatctatc
    15781 aatcggtagc cgctgaaggt aatgctgttg aaaagcaaat tccccagcca gttggtggac
    15841 gccagcgtag ctaatgaatt accgcaaatg aacagcaaac cgttagtctg gctggaaggt
    15901 gtttcatcgg tactgttcgg tgtagccgca ttctcgacct tttatgtgat tgtggtgtgg
    15961 atgcccaaat atgcgatggc ttttgctggt atgtcagaag ctgaggcatt aaaaaccatc
    16021 tcttattaca gtatgggctc gttggtctgt gtctttattt ttgccgcact actgaaaaaa
    16081 atggtccggc ccatctgggc taatgtattt aactctgcac tggcaacaat aacagcagcc
    16141 attatctatc tgtacccttc tccactggtg tgtaatgccg gcgcctttgt tatcggtttc
    16201 tcagcagctg gcggcatttt acagctcggt gtttcggtca tgtcagagtt tttcccaaaa
    16261 agcaaagcca aagtcaccag tatttatatg atgatgggtg gactggctaa ctttgttatt
    16321 ccgctgatta ccggttatct gtcgaacatc ggccttcaat atatcattgt tctcgatttt
    16381 actttcgcgc tgctggccct gattaccgca attattgttt ttatccgcta ttaccgcgtt
    16441 tttattattc ctgagaatga tgtgcggttt ggcgagcgta aatttagcac ccggttaaac
    16501 acaattaagc atagaggtta aaggagttaa ttatggatgt taccgcaaaa tacgaattga
    16561 ttgggctgat ggcctatcct atccgccaca gtttatcgcc tgaaatgcag aataaagcct
    16621 tagaaaaagc gggattgcca tttacctata tggcctttga agtggataac gatagttttc
    16681 ccggagcaat tgaaggatta aaagctctaa aaatgcgcgg aactggtgta tcaatgccga
    16741 acaaacaact ggcttgttaa tatgttgatg aattaacgcc agcagccaaa ctggtgggtg
    16801 ccatcaacac catcgttaat gatgatggct acctgcgcgg ctataacacc gacggcacgg
    16861 gccatattcg cgccattaaa gagagcggtt ttgatatcaa aggcaaaaca atggtgctgt
    16921 taggggccgg aggtgcctca acggctattg gcgcgcaggg ggcaattgaa ggtttaaaag
    16981 aaattaaact ctttaaccgt cgggatgagt tcttcgataa agccctcgcc ttcgcgcagc
    17041 gggttaacga aaacaccgat tgtgtcgtca cggttaccga tctcgccgat cagcaagcct
    17101 ttgctgaagc cctggcttcc gccgacattt taaccaatgg cacaaaagtg ggtatgaaac
    17161 cccttgagaa tgaatcatta gttaatgata tcagtctgtt acatccggga cttctggtca
    17221 ctgaatgcgt gtataacccg catatgacga agttattgca gcaggcgcaa caagctggtt
    17281 gcaaaacgat tgatggatac ggcatgttgt tgtggcaagg ggctgaacag ttcacgttat
    17341 ggactggcaa agatttccct ctggaatatg ttaaacaggt catggggttc ggtgcctgac
    17401 aggctgaccg cgtgcagaaa gggtaaaaaa atgaaaaccg taactgtaaa agatctcgtc
    17461 attggtacgg gcgcacctaa aatcatcgtc tcgctgatgg cgaaagatat cgcccgcgtg
    17521 aaatccgaag ctctcgccta tcgtgaagcg gactttgata ttctggaatg gcgtgtggac
    17581 cactttgccg acctctccaa tgtggagtct gtcatggcgg cggcaaaaat tctccgcgaa
    17641 accatgccag aaaaaccgct gctgtttacc ttccgcagtg ccaaagaagg cggcgagcag
    17701 gcgatttcca ccgaggctta tattgctctc aatcgtgcag ccatcgacag cggcctggtt
    17761 gatatgatag atctggagtt atttaccggc gatgatcagg ttaaagaaac cgtcgcctgc
    17821 gcccacgcgc atgatgtgaa agttgtcatg tccaaccatg acttccataa aacgccggaa
    17881 gccgaagaaa tcattgcccg tctgcgcaaa atgcagtcct tcgacgccga tattcctaag
    17941 attgcgctga tgccgcaaag taccagcgat gtgctgacgt tgcttgccgc gaccctggag
    18001 atgcaggagc agtatgccga tcgtccaatc atcacaatgt caatggcaaa aactggcgta
    18061 atttctcgtc tggctggtga agtatttggc tcggcggcaa cttttggtgc ggtaaaaaaa
    18121 gcctctgcgc cagggcaaat ctcggtaaat gatttacgca cagtattaac tattttacat
    18181 caggcataag caataatatt tcggcgggaa tatcctcccc cgccgaacta aaaaaaatat
    18241 attcaaccgt atttaataaa aatatttcgt gagtctctgt gcgctaaata tccatctggc
    18301 gtagggaaaa tcacacctga atcagaaatt aacaatgaaa cctgtaaaac cacctcgtat
    18361 taatggacga gtgccggtcc tgtcggcaca ggaagcggtg aattatattc ccgacgaagc
    18421 aacactttgt gtgttaggcg ctggcggcgg tattctggaa gccacgacgt taattactgc
    18481 tcttgctgat aaatataaac agactcaaac gccacgtaat ttatcgatta ttagtccaac
    18541 agggcttggc gatcgcgccg accgtggtat tagtccgctg gcgcaagaag gtctggtgaa
    18601 atgggcatta tgtggtcact ggggacaatc gccgcgtatt tctgaactcg cagaacaaaa
    18661 taaaattatt gcttataact atccacaagg tgtacttaca caaaccttac gcgccgccgc
    18721 agcccaccag cctggtatta ttagtgatat tggcatcggg acatttgtcg atccacgcca
    18781 gcaaggcggc aaactgaatg aagtcacgaa agaagacctg attaaactgg tcgagtttga
    18841 taacaaagaa tatctctatt acaaagcgat tgcgccagat atcgccttca ttcgcgctac
    18901 cacctgcgac agtgaaggct acgccacttt tgaagatgag gtgatgtatc tcgacgcatt
    18961 ggttattgcc caggcggtgc acaataacgg cggtattgtg atgatgcagg tgcagaaaat
    19021 ggttaagaaa gccacgctgc atcctaaatc tgtccgtatt ccgggttatc tggtggatat
    19081 tgtggtggtc gatccggatc aaagccaact gtatggcggt gcgccggtta accgctttat
    19141 ttctggtgac ttcacccttg atgacagcac caaacttagc ctgcccctaa accaacgtaa
    19201 attagttgcg aggcgtgcat tattcgaaat gcgtaaaggc gcggtgggga atgtcggcgt
    19261 cggtattgct gacggtattg gcctggtcgc ccgggaagaa ggttgtgctg atgactttat
    19321 tctgacggta gaaacaggtc cgattggcgg aattacttca caggggatcg cctttggcgc
    19381 gaacgtgaat acccgtgcca ttctggatat gacgtcccag tttaattttt atcacggtgg
    19441 tggtctggat gtttgttatt tgagttttgc tgaagtcgac cagcacggta acgtcggcgt
    19501 gcataaattc aatggcaaaa tcatgggtac cggtggattt attgatatca gtgccacctc
    19561 gaagaaaatt attttctgcg gcacgttaac cgcgggcagt ttaaaaacag aaattgccga
    19621 tggcaaatta aatatcgtcc aggaaggacg ggtgaagaaa tttattcagg aactaccgga
    19681 aattactttt agcggaaaaa tcgcgctcga acgggaactg gatgttcgtt acatcaccga
    19741 acgtgctgta ttcacgctga aagaagacgg cctgcattta atcgaaatcg cccctggcgt
    19801 cgatttacaa aaagatattc tcgacaaaat ggatttcacc ccagtgattt cgccagaact
    19861 caaactgatg gacgaaagat tatttatcga tgcggcgatg ggttttgtcc tgcctgaagc
    19921 ggctcattaa caggagtatc acgatggatt tttctttaac tgaagaacaa gaactgctgc
    19981 tggccagtat tcgcgaactg attacgacta actttgcgga agagtatttc cgcacctgcg
    20041 atcaaaacgg gacatatccg cgtgagttta tgcgggcgct ggcggataac ggcatttcta
    20101 tgcttggcgt gccggaagag ttcggtggca tccctgcgga ttacgtcacc cagatgctgg
    20161 cgctgatgga agtgtcaaaa tgcggtgctc cggcattttt gattaccaac ggtcaatgta
    20221 ttcacagtat gcgccgtttc ggttctgcag agcagctacg taaaacggca gagagcaccc
    20281 tggaaacggg tgatcccgcc tatgccctgg cgttgacgga gccaggcgca ggctcagaca
    20341 acaacagtgc cactaccact tacacgcgta aaaacggcaa ggtttacatc aacggacaga
    20401 aaacctttat taccggtgcg aaagagtacc cgtatatgct ggtgttggcg cgcgatccgc
    20461 aaccgaaaga tcccaaaaaa gccttcaccc tgtggtgggt cgactccagt aagcccggca
    20521 ttaagattaa cccgctgcat aaaatcggct ggcatatgct cagcacctgc gaagtctatc
    20581 tcgacaacgt ggaggttgaa gagagcgaca tggtgggcga agaaggaatg ggtttcctca
    20641 atgtgatgta caactttgag atggagcgcc tgatcaacgc cgcgcgcagc accggctttg
    20701 ccgaatgcgc ctttgaagat gccgcccgct atgccaacca acgtatcgct tttggtaagc
    20761 ccattggtca taaccagatg atccaggaaa aactggcgct gatggcgatt aagatcgaca
    20821 acatgcgcaa catggtgctg aaagtggcat ggcaagccga tcagcatcag tcactgcgca
    20881 ccagcgcggc gctggcaaaa ctgtattgcg cacgtaccgc aatggaagtc attgatgatg
    20941 cgattcaaat catgggcggt ctgggctata ccgatgaagc gcgcgtctcc cgcttctggc
    21001 gtgatgtccg ttgtgaacgt atcggcggcg gtacagacga aattatgatt tacgtagcag
    21061 gtcggcagat cctgaaagac tatcagaaca aataatctgc aggcggcgca gcttcttaac
    21121 aaactgcgcc gccagattta tccaacaaga cttaccggtt gaggaaattc agataggatt
    21181 tcttgttccg caaaacagtt tttaaattgt ctacggtaat cgctgggcga acaaccgaca
    21241 tggcgcaaaa acagtttggc aaagtgatcg acattttcat aacccacacg ccaggagatc
    21301 tctgcctgtg ataattcagt attagtgagt gaccattttg cttccgtcat acgccgttga
    21361 ataacatagt taatagggga aatacgatac tctttggtaa attcatggca aatataactg
    21421 acgctggcac gaaatttttt cgataactgt tctaaagtga ttttttcgcg ataattatta
    21481 ctcagataga aaagaatatc ttttatcaga acatcttttt taatataccc ctgctccgaa
    21541 cgataagcat ttttaaaatt ttcgtagtaa agaatggcta acgtataggc aaatgcatcc
    21601 cataacgaag atgtttggct atttttactt tgcggcaaaa tcacacttag ctcattaaat
    21661 aaggttttta tgacttcttt tccctgcccc gcggcaatta ccggacaaga atgcggttgc
    21721 agtagctgat tttcctcagc cccctgaaac tgaaagccgt acagcgcaca ggtacacgtc
    21781 gttgccggat cgttaacgtc agaggccacc gcatgcagcc tgccgcgttc tatcaccaca
    21841 atgtcatccg catgtgcgac atataacgaa gaatcgatgg taaaccttgc aacccctttc
    21901 ttgacgtaaa tcaactcggt ttcattgtca tgaacgtgat gtccggactc ccattttgga
    21961 tcatcgctaa atgcaaaacg tgaaagccgt ggcgttttac cggcgacaaa cagcgtttca
    22021 ctggcgttat caaaacagcg ttgatacatg acaacctccc tattccatga gcaagcaaaa
    22081 acaatatatg ccggataaaa tacggcgtgt attgcggtta tacaaccgcg tttagcgaga
    22141 gctaaacaag attatttaca ctgaatgcaa gattgtacgg tcatggaata actatattgc
    22201 cgcaaattgc acttttgttt tagcgatcgc atttttttgc aagattgttg gcaaggaaaa
    22261 cagcttgctc cgtcgaaaac ccccaccgct atcgcacact attttcaggc catttttacc
    22321 ttccatcgga gatggttccg tatgcgactc acaggagaaa tcatgaaaat aataacctgc
    22381 tttaagctgg tgcctgaaga acaggacatt gttgtcactc cagaatacac cctgaatttc
    22441 gacaatgccg acgccaaaat cagccagttc gatctcaatg ccattgaagc tgcaagccag
    22501 ctcgcaacag atgacgatga gatagccgcg ctgaccgttg gcggctcatt gttgcagaac
    22561 tcgaaagtgc gcagtgcgca aagatgtgct atcccgcggg ccgcacagcc tgtatttggt
    22621 gcaggatgcg caacttgaac atgcactgcc tctcgatacc gcaaaagcgc tggcggcagc
    22681 aattgaaaag atcggcttcg atttactgat ctttggtgaa ggttccggcg acctttatgc
    22741 ccagcaggtt ggcttgctgg tcggagaaat tctgcaactt ccggtgatta atgcagtgag
    22801 tgctattcag cgtcagggta atacactggt gattgaacgc acgcttgaag atgatgttga
    22861 agttattgaa ctctctgttc cagccgtgct ctgcgtcacc tccgatatta acgtgccacg
    22921 cattccttcg atgaaagcca ttctcggcgc gggtaaaaaa ccggtaaatc agtggcaggc
    22981 aagtgatatt gactggagcc agagcgcgcc acttgctgaa ctggtcggca ttcgcgtacc
    23041 gccgcaaaca gaacgtaagc acatcattat cgataacgat tcgccggagg ccattgccga
    23101 gctggcggaa catctgaaga aagccctgaa ctgaagccta cggagaagaa acgatgagtc
    23161 aattaaacag cgtctgggtc tttagcgata atcctgaacg ttatgctgaa ctgtttggcg
    23221 gcgctcagca atggggccaa caggtgtatg ccattgtaca aaataccgac caggcgcagg
    23281 cagttatgcc ttatggtcca aaatgtattt atgttcttga gcaaaacgac gcgctgcaac
    23341 gcactgaaaa ttacgccgaa agcattgctg ccccgctgaa agataaacac cccgctatgt
    23401 tgctgttggt cgcgacgaaa cgtggtaaag ctctggcagc acggttaagt gtgcaactga
    23461 atgcggcgct ggtgaacgat gccacggcgg tggatattgt cgatggtcac atttgcgccg
    23521 aacaccggat gtatggcggg ttagcgttcg ctcaggaaaa gatcaacagc ccgctggcga
    23581 tcattaccct tgcacccggt gttcaggaac cgtgcaccag tgatacctct catcagtgcc
    23641 cgacagaaac ggtaccttat gttgctccgc gtcatgaaat tctctgtcgc gaacgccgtg
    23701 cgaaagccgc aagcagcgtg gacctgagca aagcaaaacg tgtggttggc gtcggtcgtg
    23761 gtctggcggc gcaggatgac ctgaaaatgg tccacgaact ggcggcggtg ctgaatgctg
    23821 aagtcggctg ttcacgtcca attgccgaag gcgagaactg gatggagcgt gaacgttata
    23881 tcggtgtctc cggcgtgttg ctgaaatccg atctctacct gacgctgggg atctccgggc
    23941 agatccagca tatggttggc ggcaacggcg caaaagtgat tgtcgccatc aataaagata
    24001 aaaatgcgcc aatcttcaat tatgccgact acggtctggt gggcgatatc tacaaagtcg
    24061 tccctgccct gattagccag ttgagccgct aattcccctt tcaaccacat ctcccgctgt
    24121 aaacgcagcg ggaagggagc ataacgcatg tcggatgaca aatttgatgc cattgtggtc
    24181 ggtgcggacg ttgctagtag cgttgccgca ctggtcatgg cgcgagccgg actggatgtc
    24241 ctggtgatag aacgcggcga cagtgccgga tgtaaaaaca tgaccggcgg gcgtctttat
    24301 gcccacacac ttgaagcaat cattccaggc tttgcagtat cagcgccggt agaacgcaag
    24361 gtcacacgcg agaaaatctc cttcttaacc gaagaaagcg ccgttaccct cgattttcac
    24421 cgcgagcaac cagatgttcc gcaacacgca tcttataccg tattgcgtaa tcgtctggac
    24481 ccgtggttga tggaacaagc cgagcaggcg ggcgcacagt ttatcccggg cgttcgcgtc
    24541 gatgcgttgg ttcgtgaagg aaacaaggtc actggcgtgc aggccgggga tgatattctc
    24601 caagcgaatg tggtggttct agctgatggc gtaaactcga tgcttggccg ctcgctggga
    24661 atggttcccg cttccgatcc gcatcattac gctgttggtg ttaaagaggt tattggcctc
    24721 acaccagaac agatcaacga tcgctttaat attacgggcg aggaaggtgc cgcctggctg
    24781 tttgccggct ccccttctga cggcctgatg ggcgggggat ttctctatac caacaaggat
    24841 tccatatcct tggggctggt ttgtggattg ggtgatatcg cccatgcgca aaaaagcgtg
    24901 ccgcaaatgc tggaagattt taaacaacac cccgccattc gcccactaat tagcggcggc
    24961 aaactgcttg aatattccgc gcatatggtg ccagaaggcg gtctggcaat ggtgccgcag
    25021 atggttaacg atggcgtgat gatcgttggt gacgccgcag gcttctgcct gaatttgggt
    25081 tttacagttc gtggcatgga tttagccatt gcatcggctc aggctgccgc cacaacggtg
    25141 atcgccgcca aagaacgcga ggatttctcc gccagcagtc tggcgcaata caaacgtgag
    25201 ctggaacaaa gctgcgtcat gcgcgatatg cagcatttcc gcaagatccc ggcgctgatg
    25261 gaaaacccgc gcctgtttag ccaatactca cgaatggtcg ccgacatcat gaacgagatg
    25321 ttcaccattg acggcaaacc aaaccagccg gtacgcaaaa tgatcatggg acacgcgaag
    25381 aaaattgggc tgatcaactt gctgaaagat ggcattaagg gagcaaccgc gctatgagcc
    25441 agaacgctac ggttaacgtc gacatcaaat taggcgtcaa taaattccat gttgatgagg
    25501 gccacccgca tatcattctg gcggcaaatc ccgatatcaa tgaattccgt aaattaatga
    25561 aagcttgccc tgccggactt tataagcagg atgacgccgg caacattcat ttcgattccg
    25621 ccggttgtct ggagtgcggc acctgtcggg tgctgtgcgg caacactatt ctcgaacagt
    25681 ggcaatatcc cgcaggcacc ttcggtattg attttcgcta cggctaatca tgcatcccac
    25741 aggcccgcat ctcgggcctg atgttctgtt tcgagagtcc aaaatgaaag tgacattaac
    25801 gttcaacgaa caacgtcgtg cggcgtatcg tcagcaaggg ttatggggcg atgcttcact
    25861 ggccgattac tggcagcaga ccgctcgtgc gatgccagac aaaattgccg tggtcgacaa
    25921 tcatggtgca acgtacacct atagcgcgct cgatcacgcc gcgagctgtc tggcaaactg
    25981 gatgttagcg aagggtattg aatcaggcga tcgcatcgca tttcaactgc ctggctggtg
    26041 tgaatttacc gttatctatc ttgcctgcct gaaaatcggt gcggtttccg tgccgctgtt
    26101 gccttcctgg cgggaagcag aactggtgtc ggtgctcaat aagtgtcagg caaaaatgtt
    26161 ctttgcaccg acgttgttta aacaaacacg tccggtagat ttaatcctgc cgctgcaaaa
    26221 tcagcttcca caactacaac aaattgtcgg cgtggacaaa ctggctcccg ccacctcttc
    26281 cctctcatta agtccgatta tcgccga
//
LOCUS       DAJTYD010000039        26163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTYD010000039 DAJTYD010000000
VERSION     DAJTYD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.039"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(348..1259)
                     /gene="gsiD"
                     /locus_tag="OO585_001492"
     CDS             complement(348..1259)
                     /gene="gsiD"
                     /locus_tag="OO585_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6629121.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1262..2182)
                     /gene="gsiC"
                     /locus_tag="OO585_001493"
     CDS             complement(1262..2182)
                     /gene="gsiC"
                     /locus_tag="OO585_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6629122.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2200..3738)
                     /gene="gsiB"
                     /locus_tag="OO585_001494"
     CDS             complement(2200..3738)
                     /gene="gsiB"
                     /locus_tag="OO585_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6629123.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3758..5629)
                     /gene="gsiA"
                     /locus_tag="OO585_001495"
     CDS             complement(3758..5629)
                     /gene="gsiA"
                     /locus_tag="OO585_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6629124.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5616..6581)
                     /gene="iaaA"
                     /locus_tag="OO585_001496"
     CDS             complement(5616..6581)
                     /gene="iaaA"
                     /locus_tag="OO585_001496"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6629125.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6785..8020
                     /gene="moeA"
                     /locus_tag="OO585_001497"
     CDS             6785..8020
                     /gene="moeA"
                     /locus_tag="OO585_001497"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6629126.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8020..8769
                     /gene="moeB"
                     /locus_tag="OO585_001498"
     CDS             8020..8769
                     /gene="moeB"
                     /locus_tag="OO585_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6629127.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8845..9507)
                     /gene="fsa"
                     /locus_tag="OO585_001499"
     CDS             complement(8845..9507)
                     /gene="fsa"
                     /locus_tag="OO585_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6629128.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            9638..10537
                     /locus_tag="OO585_001500"
     CDS             9638..10537
                     /locus_tag="OO585_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6629129.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            10543..12975
                     /locus_tag="OO585_001501"
     CDS             10543..12975
                     /locus_tag="OO585_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6629130.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13121..13936
                     /gene="ybiV"
                     /locus_tag="OO585_001502"
     CDS             13121..13936
                     /gene="ybiV"
                     /locus_tag="OO585_001502"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6629131.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14088..15353
                     /locus_tag="OO585_001503"
     CDS             14088..15353
                     /locus_tag="OO585_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6629132.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFSREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15594..17186)
                     /locus_tag="OO585_001504"
     CDS             complement(15594..17186)
                     /locus_tag="OO585_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6629133.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17405..18325
                     /gene="ldtB"
                     /locus_tag="OO585_001505"
     CDS             17405..18325
                     /gene="ldtB"
                     /locus_tag="OO585_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6629134.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18384..19502)
                     /locus_tag="OO585_001506"
     CDS             complement(18384..19502)
                     /locus_tag="OO585_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6629135.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19499..19966)
                     /gene="mntR"
                     /locus_tag="OO585_001507"
     CDS             complement(19499..19966)
                     /gene="mntR"
                     /locus_tag="OO585_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6629136.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20152..20280
                     /gene="mntS"
                     /locus_tag="OO585_001508"
     CDS             20152..20280
                     /gene="mntS"
                     /locus_tag="OO585_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6629137.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            20552..22135
                     /gene="opgE"
                     /locus_tag="OO585_001509"
     CDS             20552..22135
                     /gene="opgE"
                     /locus_tag="OO585_001509"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6629138.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22184..22699)
                     /gene="ompX"
                     /locus_tag="OO585_001510"
     CDS             complement(22184..22699)
                     /gene="ompX"
                     /locus_tag="OO585_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6629139.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(22752..22817)
                     /locus_tag="OO585_001511"
     CDS             complement(22752..22817)
                     /locus_tag="OO585_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629140.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23052..23939
                     /gene="rhtA"
                     /locus_tag="OO585_001512"
     CDS             23052..23939
                     /gene="rhtA"
                     /locus_tag="OO585_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6629141.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24238..24741
                     /gene="dps"
                     /locus_tag="OO585_001513"
                     /gene_synonym="pexB"
     CDS             24238..24741
                     /gene="dps"
                     /locus_tag="OO585_001513"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6629142.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25145..25891
                     /gene="glnH"
                     /locus_tag="OO585_001514"
     CDS             25145..25891
                     /gene="glnH"
                     /locus_tag="OO585_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6629143.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26030..>26163
                     /gene="glnP"
                     /locus_tag="OO585_001515"
     CDS             26030..>26163
                     /gene="glnP"
                     /locus_tag="OO585_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="similar to permease component of ABC transporters;
                     mutations impair ability of Escherichia coli to transport
                     and utilize glutamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6629144.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     T"
BASE COUNT         6467 a         7033 c         6476 g         6187 t
ORIGIN      
        1 aaatagccaa cgtttttctg aataagttca aagcggctat cggcacgttt taccgcgcgt
       61 tccgatacca gttgatagtt aatgatgaaa aaaagaccaa taaataacag tgctgccaat
      121 aaaaataaaa ttatcagcct tatctttatc ttttcgtata aactcagcat gttaatacat
      181 ttaacaaaat aactatctga acgctgtgcc gctgcaaaaa tagtcccttg ctgcggcttt
      241 ttaaccaaat tacccgattc tacaaaatca gcagaaagtt acaacacttg cttagggcaa
      301 taatttcctg tgaggtgatt accctttcaa gcaatattca aacgtaacta tcctttaatt
      361 ttcggatcca gcgcatcgcg taaaccatcg cccaacaaat tgaacgccag tacggtcaga
      421 aaaatagcca gggccggaaa aacagcgaca tgcggcgcga taaccatatc cgctcgtgcc
      481 tcattgagca ttgctcccca ctctggtgtc ggcggctgcg caccgaggcc gagaaatgac
      541 aggctggcgg cagagataat cgaggtgcca atgcgcatgg tgaaaaacac cacgatagac
      601 gagacggtcc caggcaagat atgacgcaat aaaatggtca tatcgctggc accaatactg
      661 cgtgctgact caataaaggt ttgctgtttc aacaccagcg tgttgccgcg caccagtcgg
      721 gcaaacgcgg ggatggaaaa aatggcgact gcgataatca cgttagcaat gccgcttccc
      781 aacaccgcaa caacagcgat cgccagtaaa atacccggga aggcaaacag cacatcgcaa
      841 atgcgcatga tcagccgatc ccaccagcct tcataatatc cagccaacaa gcccagcaac
      901 gtcccgatcg ccgcaccgat aaacacggca aacacgcccg ccgccagcga gatttgcgca
      961 ccaaccagga cacggctgaa aatgtcgcgc cccagtgaat cgacgccaaa ccagtgctgc
     1021 aaagaaggtc cgttattcag attgtcataa tcaaaataat tttcggcgtc atagggagcg
     1081 atccagcgtg caaaaatggc caccacaatc aataaaataa cgaataatgc ggcggtcatc
     1141 gccatatgct gacggcgaaa tcgtcgccag aattcatgcc acggtgtacg cacctggtca
     1201 ggtttgacca gtggcatggc gtttaacacc gcctgacgtc gccagttaaa tagtcgcatc
     1261 cttacttgta cctgatagcc gggttaatgg cggcgtaaag cacatccacc actaagttga
     1321 taagaataaa ttccagcgag aaaagcagaa tttccgcctg aatcaccggg taatcgcgca
     1381 tttctacgga gtcaacgagt aagcgtccaa gtccgggcca gttgaaaact ttctccacca
     1441 cgatagaacc accgagcaaa aagccaaact gtaagcccat catggtcact accgggatca
     1501 tcgcgttacg tagcccgtgt ttgaggacaa cccaggtttc gctcacccct ttcgccctcg
     1561 cggtacgcat ataatcttcg cttaaaacat cgacaaacga cgcgcgggta aagcgcgcca
     1621 tcacggctgc cactgccgcg ccgagcgtca gggaggataa aatgtagtgc tgccaactgt
     1681 ctgctcccac ggtaggcagc cagcccagtt caacggagaa tacctgaatt aaaagcatcc
     1741 ccagcgcaaa tgccggaaac gagatccccg acaccgcaat ggtcatactc aatcgatccg
     1801 gccaacgatt acgccagacg gcggcgataa ttcccgccgc cataccaaat atcaccgccc
     1861 agaccatact ggttatggtc agccacagcg ttggcataaa gcggctggca atctcatcgg
     1921 caaccggacg acgcgacacc atcgacaggc caaaatcccc ctgcacagca ttgctgatat
     1981 agtgccagaa ctggtgatac agcggctgat ccaaccccag ctgctgacgc accagttcta
     2041 taacctgcgc atcagcttcg ggcccggcaa tcaatcgcgc cggatcgccg ggcagcatat
     2101 gcacaaataa aaacaccagc accgagacga taaacagcgt cggaatcaac cccagtaagc
     2161 gtttgataac gtaattaagc attccactcc ctgcgttgct tattgcaaat ccgcgtcttc
     2221 aaagctgaag ccggtgtctg gcatgatcca aaaaccggtc aggtttttac tgtgtgccga
     2281 caccagtttt tctaccacca gcgggatcca cggcgactct tgccagatga tatcctgcgc
     2341 cgccttatac aagcgggtct tttccgccgg atcattagtt ttcagtgcct gagccaggaa
     2401 gtcgtccacc tgtttattgc tgtaaaatgc ggtattaaac agcgtcggtg gccagttttg
     2461 cgaggcgaac agtggcgata gcgcccagtc cgcttcgccg gttgaagccg accagccagt
     2521 gtagaacatc cgcacgccgc tctctttttg ccctttacct tcaacttctg ccgcccgctg
     2581 tccggcatcc atcgcggtca cctgggcttt aatcccgacc tgcgctaact gctgctgggt
     2641 aaattgcagc actttctgcg cggtgctgtg gttatgtgac gaccacagcg tggtactgaa
     2701 accgttggga tatcccgcct ctttcagtaa ttcgcgcgct ttcactggat cgtaaggcca
     2761 cggtttataa ctttgcgcgt aggcgatact tggcggtacc acaccagtag ctggcgttgc
     2821 atagcccgca aaggcaactt tcaccagcgc cggacggtta atggcgtaat tcagcgcctc
     2881 acggaccttc gggttatcga acggcttttg cgtcacgttc atactgatat aacgctgcat
     2941 aattgacgga ctggccatca actcgatatt tttgtttttc tccagcagtg cggcctgctc
     3001 gtagggaatg gggaaagcaa actgcgcttc accggtttgc agcattgccg cgcgggtgtt
     3061 gttatccgcc accggacgcc aggttatgct gtccagtttg ggcaatcctg gctgccagta
     3121 acccgcgaat tttttcacct tcacaaaatc ggtctgattc caggtatcca gttcatacgg
     3181 tccggttccc accggatgaa aaccaatctc cttgccatat ttttccagcg ctgccggtga
     3241 aatcatcgcg gtcgccggat ggacaagaat attaataaac gctgagaacg gctgtttgag
     3301 ggtaatcttt accgttgtcg gatcgatcgc ctccgttttg gcaatattct tatacaggtt
     3361 atagcgttta agatgattcg ccggatcgct ggcccggtcc agattcgctt tcaccgccgc
     3421 ggcgttgaaa tcggtgccat cctggaattt aattccttcc cgcaatttca cggtgtaagt
     3481 aatgccgtca tcggaaacgg tataactctc cgccagcacg tttttcagtt tcatctcttt
     3541 atccagaccg aacagcccct ggtaaaacga tttcgctacg gcctgagata acgtgtcatt
     3601 cgcgtcatac ggatcgagcg tggtgaaatt cgatcctacc gccaccacca catctttggc
     3661 agcgaatgca caagatgcca tcaacgctgt cgcaatgccc agcgccacta acccactacg
     3721 gtgtacagct cttgccattt tattctccgc ctgaatgtta tctacgcatg aatgcgtatt
     3781 ctgattgtgg ttgtgcgacg taatgccccg gcccgacgca ttgcaacgag acggctgcca
     3841 cctcttcgcc acgcagatga atattgctgg gaagatcgtc cgacagcagt acacgctgcg
     3901 gtcgttgtcg ggacggttca gcgaccggaa ctgccgccag taatttacgc gtataaggat
     3961 gctgcgggtt ttcgaagacc gcgcgccgtg gaccaatttc aacaatttgc ccgagataca
     4021 tcaccgccac acgatgacta atccgctcta ccacggccat atcgtgggag ataaacagat
     4081 acgcaatgcc gaaatcacgc tggagatcga gcaacaagtt gataatctgc ccgcgaatag
     4141 aaacatccag cgccgaaacg gcttcgtcgg caatgatcac ttttggattc aatgccaacg
     4201 cgcgagcaat gcagatgcgc tggcgctgac cgccggaaaa ctcatgcggg taacgccagg
     4261 catgttcagg taacaggccc acgcgctcca gcaaccacgc aacgcgtgca gccgcgtctt
     4321 tacctggcaa taaaccgtgt acacacagcg gttcgataat cgaatcaccg atggtctgac
     4381 gtgggtccag cgaagcgtaa gggtcctgaa aaataaactg aatatcccgg cgcaatgcct
     4441 gaagtttgcc aggtgacaag gtatcgattc gctgaccgtt aaagataatt tcgccgccct
     4501 gcgattcgac caggcgcagc aacgcccgcc cggtagtgga tttaccgctg ccagactcgc
     4561 ccaccagcga tagcgtttcg ccaggccaga gatcaaaact gactttctca acggcatgca
     4621 cttcccgcgt tacgcgattc aacaaaccgc tgcgcaaagg gaaacgggtg acaagattac
     4681 gcactcgtaa aacaggttcg ccatccacca ccgttttctg ctcgatgggg ggttcctgtt
     4741 tcgctggatg ttcaagcgat atcaatggga aacgtcgggg ataatctaac cctttcatcg
     4801 caccaagttg cggaacagca gctaacagcg cacgggtgta aggatgttgc ggtgcatgaa
     4861 aaatctgttc gacggtaccc gtttccaccg cctcgccctg atacatcacc agtacccgat
     4921 cggctatctc tgccaccacg cccatatcgt gcgtgataaa gataacgccc atcgacatct
     4981 ctttttgcaa tactttgatt aattgcagga tctgcgcctg aatagtgaca tccagcgcgg
     5041 tggttggctc atcggcaatc agcaccgccg ggcggcatga cagcgccatc gcaatcatca
     5101 ctcgctggcg catcccgcca gagagttgat gcggataacg tgaaagaatg gtttgtgcct
     5161 caggaatgcg tacctgatcc agcatccgct tcgcctcgac catcgcttct tcacgactgg
     5221 cgttctgatg cagacgaatt gattcggcaa tctgttcacc cacagtaaat accgggttca
     5281 gcgatgtcat cggctcctga aatatcatcg ccatatccgc accgcgaaca tggcgcattt
     5341 gtgcagcgct ctgctcgcta agttcaatca cttcgcgact gcgccgctgc aacagcattt
     5401 tatcgcactg tactaaaccg cccgcctgtt ccaacaggcg catcaatgcc aacgcagtca
     5461 ctgacttacc ggagccggat tcgccaacaa ttgccagcgt ctcaccgcgt tgcagactaa
     5521 aagagagatt gcggaccgca gctattttct gctggtcctg cataaaggca atattcagat
     5581 tttcaaccgc cagcacatta ccggcatcaa gttcatcact gtgtggcaac ggtgtcccct
     5641 ttttcacggt agataccggt ggttggcgta tcgcctgcgt agccccaggc gcgatacatt
     5701 ccttcggtgt taaacggtag cgcgacattc ccttcatggc cgatagcgat taagccaccg
     5761 ctaccgccaa gcgcagggag tttttccatt actacccgct cgcaggcttc cgcgagactt
     5821 aatccgccgt aatccattaa cgcggcgatg tcatatgccg ccagcgcgcg gatgaagact
     5881 tcgcccgtgc cggtacaaga aaccgccaca ctggcgttat tggcgtagca tccggcaccc
     5941 actaagggac tatcgccaac tcgtccgggt aatttattgg tcattccgcc tgtggacgtg
     6001 gctgccgcca aattgccgtc taaatccaac gccacggccc ccacggtgcc cattttctgt
     6061 ttttcatcca gtggcgcacc gctatggtcg aggacggttg ccccttcctt gcgcgctgcc
     6121 agtagttgtt cataacgcaa aggcgtggag aaaatcgccg gcgagacgcg ctccatgcca
     6181 cgagcaaacg caaaattttc tgccccttcg ccaatcatca tcacatgcgg gctttgctcc
     6241 atcaccagcc gggcggcaag aaccggatta cgcagatgac taacgcccgc caccgcaccg
     6301 gctttcaggg tgttaccatc catcacacag gcgtccagtt catgggtttc atcacgcgta
     6361 aagacagcgc caattccggc gttaaacagt ggacactctt ccagcagacg caccgcttcc
     6421 gtcaccacat ccagcgcact ttcgcccgct tccagcattt tctgcccggt ttcaacaatg
     6481 gcagacaacg cctcaatgta gcgtaattcc tgttgcagac tcatctgcgc gcggctaatt
     6541 gcacctgcgc caccatgaat tgcaatgact gctttgccca tagtattcgt ccattaaggg
     6601 tggctcgcca cttttgctat aaatatcaga atttttatga attactcatt caatttttga
     6661 atatagaaag atggatatgg catgtaaagg caggagccaa cgctacgccc gaggtggcat
     6721 acagtctgtg gcgtttttct gacataatag gcaaattcga ttttgcctcc gcaggagtgt
     6781 tttcatggaa tttaccaccg gattgatgtc gctcgacacc gcgcttaatg agatgctttc
     6841 tcgcgtcacc ccactgaccg cccaggaaac gctgccactg gtacagtgtt ttggtcgtat
     6901 tctggcgagc gatgtcgttt cgccgcttga tgttccgggg tttgataact ccgcaatgga
     6961 cggctacgcg gtgcgtttag ccgatattgc ctccgggcaa ccgctgcccg ttgccggtaa
     7021 atcctttgcc ggtcagccgt atcatggtga atggcctgcg ggcacctgca ttcgtattat
     7081 gaccggtgcg ccggtgccgg aaggctgcga agcggtggtg atgcaggagc agactgaaca
     7141 aacggacaat ggcgtgcgtt ttactgctga agtgcgtagc gggcaaaata ttcgccgtcg
     7201 cggtgaagat atctctgcag gtgcggttgt tttcccggcg ggaactcgcc tgactaccgc
     7261 agagctgcca gtaattgctt cactggggat tgccgaagtt ccggtgattc gtaaagtgcg
     7321 tgtagcgctt ttttctaccg gtgatgaact ccagttgccc ggtcagccgc tgggtgacgg
     7381 ccaaatctac gacaccaacc gtctcgccgt acacctgatg ttagaacagc tgggttgcga
     7441 ggtaatcaac ttagggatta tccgcgacga tccccatgcc ctgcgcgccg catttattga
     7501 agccgacagc caggcggatg tagtgatcag ttccggcggt gtttcagtgg gtgaagcgga
     7561 ttacaccaaa acgattcttg aagagctggg ggagatcgcc ttctggaagc tggcgattaa
     7621 accaggtaaa ccgttcgcgt tcggtaaact cagcaatagc tggttctgcg gcctgccggg
     7681 caacccggtt tcagcgacgc tgaccttcta tcaactggta cagcctttgc tggcaaaact
     7741 aagcggcaac accgccagcg gcctgcccgc gcgccagcgc gtacgcacag cgtcccgcct
     7801 gaaaaaaacg ccaggacgtc ttgatttcca gcgcggcgtg ctgcaacgca acgccgacgg
     7861 tgaacttgaa gtgacgacca ccggacatca gggttcacat atatttagct cctttagtct
     7921 cggcaactgc tttatcgtgc tggaacgcga tcgcggcaat gtggacgtgg gcgaatgggt
     7981 ggaagtagaa ccgtttaacg cgttgttcgg aggcctgtaa tggcggaact cagcgatcag
     8041 gagatgctgc gctacaaccg gcaaatcatt ctgcgcggtt ttgattttga cggtcaggag
     8101 gcgctaaaag attctcgtgt gctgatcgtc ggcctcggtg gactcggctg cgcagcttcg
     8161 cagtatctgg caagcgccgg tgtcggcaac ctgacgctgc tcgacttcga cacggtttcg
     8221 ctctcgaatc tgcaacgcca gacactgcac aatgatgcca cggtcgggca accgaaggtg
     8281 gaatccgccc gtgacgccct gacgcggatc aacccacata tcgcgattac gccagtcaat
     8341 gcactgctag atgacgcaga acttgcagca ttgattgctg aatacgatct ggtgctcgac
     8401 tgtacggata acgttgcggt acgtaatcaa ctgaacgcag gctgttttgc cgcgaaggtg
     8461 ccgctggttt ccggcgcggc aattcgtatg gaagggcaaa tcacggtctt cacttatcag
     8521 gacggcgaac cgtgctatcg ctgccttagc cgtttgtttg gcgagaacgc gttaacctgc
     8581 gtagaagcag gcgtgatggc accgttgatc ggcgtaattg gttcgttgca ggcaatggaa
     8641 gcgatcaaac tgctggcagg ttatggcaaa ccggccagcg ggaaaatcgt catgtacgat
     8701 gcgatgacct gtcagtttcg cgaaatgaaa ctgatgcgta atccggggtg tgaggtgtgc
     8761 gggcagtaat tgccacctcc aggccagata agacgcggca ctccgccacg tcatcagcaa
     8821 tttatgagga tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc ccagccagtc
     8881 ctgttcaaac ttcgccacag cggcatcaac cgccggatag ctaatcatct gttgtgccac
     8941 atccagtggc agcgtaattg attcacatcc tgccagtaag cagtccagcg cctgacgcgg
     9001 gtttttgaaa ctcgctgcca gcactttcgc ctgcggcgca tgcattttca ataactggtg
     9061 taagtcggtc acagtctgaa tgccgctacc gccctgagca tcaatacgat taacgtaagg
     9121 cgcaacatat tccgcacctg ccagcgccga cagcagccct tgtgctgcgc catataccgc
     9181 ggttcccagc gttggaatcc cttccgcttt taacatctta atagctgcca gcccctcggc
     9241 ggtcaccgga actttcacca cgatatccgc aataatagaa cgcaatttac gtgcgtcatt
     9301 caccatccct tcagcagtgg tagccattac ctgggcaaac agacgcccct gaccgcccat
     9361 cgcttcatga agttgcggaa gcacaacatc cagcggtttt ttacccgcgg cgataatgct
     9421 tgggttagtg gtcacacccg ccagcggaaa aatacgtgac agcgccttca ctgcaacaac
     9481 gtctgaagta tccagataca gttccataac catcctcaaa atgtactttt tgtctgaatg
     9541 tagactatca cgggaaaact ctctgaacag ttgacgcaca tcaatccaac tttcattcga
     9601 aagtatttta atgtttgaac gaaacagaga ggcaactatg attttcaata ttcagcgcta
     9661 ctcgacccat gatggccccg gtatccgcac ggtcgtattt cttaaaggct gttcgctggg
     9721 ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg caggatctgc tgtatgacgc
     9781 acgactgtgt ctggaaggct gcgagctgtg cgctaaggca gcgccggaag tgattgagcg
     9841 cgcattgaat ggtttgctta ttcatcggga aaagttaacc ccggagcatc tgacggcgct
     9901 aaccgactgc tgcccgacac aggcgttaac cgtgtgtggg gaagtgaaaa gcgttgagga
     9961 gatcatggcg accgttctgc gcgataaacc gttttacgat cgcagcggcg gcggcttaac
    10021 gctttcgggt ggtgaaccct ttatgcagcc ggaaatagcg acggcgctac tgcaagcctg
    10081 ccacgaggca ggcattcata ctgcggtaga aacctgtctg catgtgccgt ggaaatatat
    10141 caccccttct ctgccctata tcgatctgtt tcttgccgat ttaaaacaca ttgccgacgc
    10201 gccgtttaaa cagtggaccg acggtaacgc cgccaaggtg ctggataacc tgaaaaaact
    10261 cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg attcagggct ttaatgccga
    10321 cgaaacctct gtaaaagcca ttaccgattt tgccgccgac gagctgcacg ttggcgaaat
    10381 tcattttctg ccctaccaca cgctgggcat caacaaatat cacttactta atctgcccta
    10441 tgacgccccg gaaaaaccgc ttgatgcgcc agacctgctc gactttgccc agcagtatgc
    10501 ctgccagaaa gggttaaccg cgaccttacg aggataacaa ccatgaccac actgaaactg
    10561 gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat tgtgaaaccg
    10621 ccggtctgta ccgagcgcgc gcagcactat accgagatgt atcaacaaca tctcgataag
    10681 ccgatcccgg tacgtcgcgc gctggctctg gcgcatcacc tggcgaatcg caccatctgg
    10741 atcaagcacg atgagttgat catcggcaac caggcaagcg aggttcgcgc tgcgccgatc
    10801 ttcccggaat ataccgtctc gtggatcgaa aaagagattg atgatctggc agatcgtccc
    10861 ggtgctggtt ttgcggtgag cgaagagaac aaacgcgttc tgcatgaagt gtgcccgtgg
    10921 tggcgcggtc agacggtaca ggatcgctgc tacggcatgt ttaccgatga gcaaaaaggt
    10981 ctgctggcga ccggaatcat taaagcggaa ggcaatatga cctccggcga tgcgcacctg
    11041 gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg gtctgcgcga gaaagtagcg
    11101 gaacgtcgct cgcgcatcaa tctgacggta ctggaagatt tgcacggcga acagttcctg
    11161 aaagcgattg atatcgtgct ggtggcagtc agcgaacaca ttgaacgttt tgcagccctg
    11221 gcgcgtgaaa tggccgcgac cgaaacccgc gaaagccgtc gcgatgaact gctggcgata
    11281 gcagaaaact gcgatcttat cgcccaccag ccgccgcaga ctttctggca ggcgctgcaa
    11341 ctgtgttact tcatccagtt gatattgcag atcgaatcta acggtcactc ggtatcgttt
    11401 ggtcgtatgg accagtatct ctacccgtac tatcgccgag acgttgaact caaccagaca
    11461 ctggatcgcg aacacgccat cgaattgctg catagctgct ggctgaaact gctggaagtg
    11521 aacaagatcc gctccggctc acactcaaaa gcctctgcgg gaagtcctct gtatcagaac
    11581 gtcaccattg gcggacaaac tctggttgat ggtcaaccaa tggacgcggt gaatccactc
    11641 tcttacgcga tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa cctcagcgtg
    11701 cgttaccatg caggaatgag taatgatttc ctcgacgcct gcgtacaggt gatccgttgc
    11761 ggcttcggga tgccggcgtt caacaacgac gaaatcgtga tcccggaatt tattaaactc
    11821 ggtattgaac cgcaggacgc ttacgactac gcagcgattg gttgtatcga aaccgccgtc
    11881 ggtggcaaat ggggctatcg ctgtaccggc atgagcttta tcaacttcgc ccgcgtgatg
    11941 ctggcggcgc tggaaggcgg gcgtgatgcc acaagcggca acgtgttcct gccacaagaa
    12001 aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag taatggacgc gtgggatacg
    12061 caaatccgtt attacacgcg taaatcaatc gaaatcgaat atgtcgtcga caccatgctg
    12121 gaagagaacg tgcacgatat tctctgctcg gcgctggtgg atgactgtat tgagcgagcg
    12181 aaaagtatca agcaaggcgg cgcgaaatat gactgggttt ctggcctgca ggttggcatt
    12241 gccaacctcg gcaacagcct ggcggcagtg aagaaactgg tatttgaaca gggcgcgatt
    12301 ggtcagcaac agctggctgc cgcactggcg gatgacttcg atggcctgac tcatgagcaa
    12361 ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca acgacgatga tactgtcgat
    12421 acgctgctgg ctcgcgctta tcagacctat atcgacgaac tgaaacagta ccataatccg
    12481 cgctacggtc gtggtccggt tggcggcaac tattacgcgg gtacgtcatc tatctccgct
    12541 aacgtaccgt ttggtgcgca aactatggca acgccggacg ggcgtaaagc ccacaccccg
    12601 ctggcagaag gcgctagtcc ggcctctggt actgaccatc tcggccctac tgcggtcatt
    12661 ggctcggtgg gtaaactgcc tacggcagcg attctcggcg gcgtgttgct caaccagaaa
    12721 ctgaatccgg caacgctgga gaacgaatct gacaagcaga agctgatgat cctgctgcgc
    12781 accttcttcg aggtgcataa aggctggcat attcagtaca acatcgtttc ccgcgaaacg
    12841 ctgctggagg cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg tgtcgcgggt
    12901 tattccgcct tcttcaccgc gctctctcca gacgctcagg acgatatcat cgctcgtact
    12961 gaacatatgc tgtaatctct tcggcccggc tgcggcgtcg ccgggctaaa tcattcacat
    13021 catcaatttc atccttactt tcattcgaaa tataatttgt gctctgcgtc acattgttat
    13081 tacactgtta cggtttcatt ttcgagccag gagcacacct atgagcgtaa aagttatcgt
    13141 cacagacatg gacggtactt ttcttaacga cgccaaaacg tacaaccaac cacgttttat
    13201 ggcgcaatat caggaactga aaaagcgcgg cattgagttc gttgtcgcca gcggtaatca
    13261 gtattaccag cttatttcgt tctttcctga gctaaaggat gagatctctt ttgtcgcgga
    13321 aaacggcgca ctggtttacg aacatggcaa gcaactgttc cacggcgaac tgacccgaca
    13381 tgaatcgcgg attgttattg gcgagttgct aaaagataag caactcaatt ttgtcgcctg
    13441 cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa gcatttgtcg cactgatggc
    13501 aaaacactac catcgcctga aagctgtaaa agattatcag gagattgacg acgtactgtt
    13561 caagttttcg ctcaacctgc cagatgaaca aatcccgtta gtgatcgaca aactgcacat
    13621 agcgttcgat ggcattatga aacccgtcac cagtggtttt ggctttatcg acctgattat
    13681 tcccggtcta cataaagcaa acggtatttc gcggttactg aaacgctggg atctgtcacc
    13741 gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg gagatgctga aaatggcgcg
    13801 ttattccttt gcgatgggca atgctgcgga aaacattaaa caaatcgccc gttacgctac
    13861 cgatgataat aatcatgaag gcgcgctgaa tgtgattcag gcggtgctgg ataacacatc
    13921 cccttttaac agctgacctc tctaccggag ccgtgcgctc cggttttccc ttctttttac
    13981 atcctgtaca tcacatcaaa tcattaaact tgcattaact tatttataac ttatattgtc
    14041 acatgcaaat cataatggtt tcgaatgaaa gttaatgaga ggtggttatg gcttctactt
    14101 ttaccagcga cacattgcca gccgatcaca aagcagctat ccgtcagatg aagcacgcgc
    14161 tgcgggcgca gcttggcgac gtccagcaga tctttaatca gctaagcgat gacattgcca
    14221 cgcgagtggc tgaaatcaac gcactcaaag cacagggcga tgccgtctgg ccggtgctgt
    14281 cttatgccga tatcaaagca ggtcatgtta ctgcagagca gcgcgaacag attaaacgtc
    14341 gcggttgtgc ggtgataaaa ggccatttct cccgcgaaca agcgctaggc tgggatcagt
    14401 cgatgctgga ctatctggac cgcaaccgct ttgacgaggt ctacaaaggc cccggcgata
    14461 atttcttcgg gacgctcagc acttcacgtc ccgagattta ccccatctac tggtcgcaga
    14521 cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc gcagtcgttt ctcaatcgtc
    14581 tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc gtcatctacc
    14641 ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa aggtcttgga gcgcataccg
    14701 actccggggc gctggaacgc tggctgcttc cagcgtatca gcgcgttttc gccaacgtct
    14761 ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc acatcgtacg gaagttgaag
    14821 agtacacggt ggacaacacc accaaatgtt ccgtgtttcg gacattccag ggctggacag
    14881 cgctctctga tatgctgcct ggtcaggggt tgctgcacgt tgtgcccatt cctgaagcca
    14941 tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa ctgtgcggcg
    15001 tagcgcccgg aagagtgttg ccggtatcag agcaatggca tccactgtta attgaggcgt
    15061 taaccagcat tccaaaactc gaggccggag actccgtctg gtggcactgc gacgtcatcc
    15121 attccgttgc ccccgttgaa aatcaacagg gttggggcaa cgtgatgtac attcctgcgg
    15181 caccgatgtg cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca ctggaaaaag
    15241 gcgcatcgcc gggcgacttc ccgcacgagg actatgaaac aaactgggaa ggacgcttta
    15301 cgcttgccga cctcaacatt cacggtaagc gagcgttggg catggatgtt tgagagttaa
    15361 tggaagtgaa ttgatgtggg attaagaaaa tcgattcgac gttactcaga ttgctgacaa
    15421 gtgcgctttc ttaatgcctg atgcggcgtt aacgccttat ctggcctaca aagtcctgca
    15481 gattcaatat attacaggta ctgcgtaggc ctgataagcg tagcgcatca ggctattttg
    15541 catttgtcat cagccgccgt caccccgaca gtatcggggt gacggataat cacttactcg
    15601 atccctttac tacgcaggta atcttcgtaa ttaccgctaa agtcgatcac gcgttccggg
    15661 gtgatttcca gaatgcgggt cgccagggag cttacgaact cacggtcgtg tgaaacaaag
    15721 atcagcgtgc cctgatacag ttccagcgcc atgttcagtg actcgatgga ttccatatcc
    15781 aggtggttgg tcggttcgtc catgatcaga atgttcggct tctgcatcat taacttacca
    15841 aacagcatcc gccctttttc cccaccggaa agcactttag ctggcttttt gatgtcgtcc
    15901 tggctgaaca gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc gccttcctgc
    15961 ttccactgac tcatccattc gaacacggtc agatcatttt caaactcata ttcgtggtcc
    16021 tgggcgtagt aaccaatgcg cgcgttttca gaccatttta cggtgccgct gtccggttgc
    16081 agatcgccca ccagcgtttt catcagcgtt gatttaccga cgccgttggt acccagtacc
    16141 gcaagttttt caccgacctc cagcagcagg ttgagatttt taaacagtgg accgttatca
    16201 aaccctttgg tcagaccttc cacttccagc gcgttacgga acagtttctt atcctgttca
    16261 aaacggatga acgggttctg acggctggag gctttcacct cttccagttt gattttatca
    16321 atctggcgcg cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct aaagcggcta
    16381 acgaaagatt gcaactcagc aatctgcgct ttcttcttgg cgttatcggc cagcagacgt
    16441 tcacgcgcct gggtcgccgc cgtcatgtac tcatcgtagt tacccggata aacgcgcagc
    16501 tcgccgtaat ccagatccgc catgtgggta cagaccatgt taaggaagtg acggtcgtgc
    16561 gagatgatga tcatggtgct gtcacgctcg ttcagcacct gttccagcca gcgaatggtg
    16621 tcgatgtcga ggttgttggt cggttcgtcg agcaggagaa tatccggatc agcaaacagc
    16681 gcctgcgcca gaagcacacg cagcttccag ccaggagcaa cttcactcat cgggccgtag
    16741 tgctgttcca ctggaattcc cacgccaagc aacaattcac cggcgcgagc ttccgcagag
    16801 taaccgtcca tttcgccgta tttaacttcc agatcggcca ctttatagcc gtcttcctca
    16861 ctcatttccg gcaaagcata gatgcggtca cgctcctgct tcacttccca caactcttta
    16921 tgccccatga tcaccgtatc cagcacagtg aactcttcaa aggcaaactg atcctgacgc
    16981 agcttaccaa tgcgctcgtt gggatcgagg gaaacgttac ccaaagtcgg ctctaagtcg
    17041 ccgccgagga tcttcataaa ggtggattta ccactaccgt tcgcgccaat caggccgtaa
    17101 cggttgccgc cgccaaattt gacggaaatg ttttcaaaca acggcttact gccgaactgc
    17161 atggtgacgt tactggaaac taacacaggc gtatcctgaa aagagatatg acaaaccgcg
    17221 cattatgcca taagtctgaa agaagatcct gctcctcgct taaggtctac actcaagcca
    17281 aaggcaaaaa atgtgatttc gtacacatct gatttcactg tgagctggaa tgaacttata
    17341 atgcgcttcc aatactctaa tattctcaac ccaatggcct gccaggcaca aaatctcgct
    17401 taacatgaat atgaaattga aaacattatt cgcagcggcc ttcgctgttg tcggcttttg
    17461 cagtaccgcc tctgcggtaa cttatcctct gccaaccgac gggagtcgcc tggttggtca
    17521 gaatcaggtg atcaccattc ctgaaggtaa cactcagccg ctggagtatt ttgccgcgga
    17581 gtaccagatg gggctttcca atatgatgga agcgaacccg ggtgtggata ccttcctgcc
    17641 gaaaggcggt actgtactga acattccgca gcagctgatc ctgccggata ccgttcatga
    17701 aggcatcgtc attaacagtg cagagatgcg tctgtattac tatccgaaag ggaccaacac
    17761 cgttatcgtg ctgccgatcg gcattggtca gttaggcaaa gatacgccta tcaactggac
    17821 caccaaagtt gagcgtaaga aagcaggccc gacctggacg ccgaccgcaa aaatgcacgc
    17881 agagtaccgc gctgcgggcg aaccgcttcc ggctgtcgtt ccggcaggtc cggataaccc
    17941 gatgggtctg tatgcactct acatcggtcg cctgtatgct atccatggca ccaacgccaa
    18001 cttcggtatc ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg aagacatcaa
    18061 attcctgttc gagaaagtac cggtcggtac ccgcgtacag tttattgatg agccggtaaa
    18121 agcgaccacc gagccagacg gcagccgtta tattgaagtc cataacccgc tgtctaccac
    18181 cgaagcccag tttgaaggtc aggaaattgt gccaattacc ctgacgaaga gcgtgcagac
    18241 agtgaccggt cagccagatg ttgaccaggt tgttcttgat gaagcgatta aaaaccgctc
    18301 cgggatgccg gttcgtctga attaatcttc aaaacttaaa gcaaaaggcg gactcataat
    18361 ccgccttttt tatttgccag accctagttg gccgggagta taactaacaa aacatatccc
    18421 accaacgccg cccacaacag catcggtatt gaatagagat ggaaacgcca ccagatgcgg
    18481 cgatcgttcg ccatacgtag cgcaatcaaa cttgccagcg atccgggtaa taacccaaag
    18541 ccacctacgt ttaccgccca tgccagtaat aaagacggcg gcacatagtt cagcaacaat
    18601 atggtactcg gcacgttact gatcacctgc gataaaccga ttgccgttaa ccataacccg
    18661 ggttcagata gatgactcac gttacccaac acgccttgca acgctggaag ctgggtcagt
    18721 aaatggacgt cgataaacat cgccataaac accagcagca gcgtccagtc cacactgagc
    18781 accacgcgac gtgccagcag cgcaaagcct gccgccacaa tcaccagtcc ccacagctct
    18841 tgtttgaact ccagcgccgt tagaaagacg atatacagcc ccagacaact ccacaccagc
    18901 cgcggtttcc actccggtgt ttgcaccccc gtatggtatt gcagcacctt tccagggaaa
    18961 caacaccagc acaggagcag gagcgtcagc atcattgcgc cagccagcgg tgccatttgg
    19021 gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt ttgcgggttg
    19081 ccaattggcg tcagtagcga accagcattg actgccagcg cctcaaaaat aatcagacga
    19141 ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac aataaacagc
    19201 gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat aaacatcgcc
    19261 agccgacgct ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc aaaataaccg
    19321 cttaactcca cacctttggt cagcagcatc aggccgctta aggtgatgat ggtgtgccag
    19381 tcgatagcag caggccagga tttcggtgca aagggcacga aaaagcttaa tccgatacca
    19441 acaagaatta ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa aggcaggctc
    19501 atttggcacc gtgtttctgg gtaaacaaac gaaaagcgtc gagcgtctct tcactaacat
    19561 ggtgctccat gccttccgcg tcgcgacggg cgatttccgg actgacgccc aacaccaaca
    19621 agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc tctccttctg
    19681 ccgttaaaaa cacgccacgc caggggatca tttcaatcag ccccatggtt gccagccgct
    19741 taagcatttt agccaccgtc ggttgcgaaa ctcccagacg agcagccatg tccacctgac
    19801 gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca tcaataagct
    19861 cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc acgttcacta
    19921 actgcgtcac tttttttgct gttggcgtac ctgcgcgacg actcattgtg cttcctcatt
    19981 atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag agttgcgaag
    20041 gtacaaaaaa ttaacgtttt agcaatagct atataatata gcctgtgcta tatctgtatg
    20101 taatgcaatc atccctcaag gatcgacggg attagcaagt caggaggtct tatgaatgag
    20161 ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt aatactgctc
    20221 tctatgttgt gcgatatggt caacaacaaa ccgcagcaag ataaaccttc cgataaatag
    20281 cggcgtcgcg gtacgccgct tcactcctgc tttcatgcag gcataacgcg ttttggcctg
    20341 aaaaacccca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt tcgtcagtag
    20401 ttgacctgaa cggcggctcg ctctatcttc ttgcagccct gcgtatattg cggctcgcgg
    20461 atgcggaccc ctttccactc ttcacgcact cttgcaggta ttgacccttg acgccagggt
    20521 aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag aatcgcttgt
    20581 tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt cgctattaat
    20641 taacctcggc ttaggttacc ccttcagttt gctctacacc gctgcgttta cggctatttt
    20701 gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg tcagttcgct
    20761 ggtggcggct tgttatttcc cttttgctca ggcctacggc gcgcctaatt tcaatacatt
    20821 gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga tttttccgtg
    20881 gtacagctac ctggtcggct tatttatttt tgcgctcggc gtaatagcaa tcaggcgaaa
    20941 aaaagagaat gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg tattcagtgt
    21001 ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat ttaaactgaa
    21061 agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata ataacgaggt
    21121 gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatacct ggacggtcac
    21181 tgccgttaag ccgaagtatc agacctatgt ggtggtgatc ggtgaaagcg cgcgtcgcga
    21241 tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca gcgttaacgg
    21301 tttgatattt gctgactaca ttgccgccag tggctccacg cagaaatcgc ttggcttaac
    21361 gctcaatcgc gtagtcgatg gcaaaccaca gtttcaggat aactttgtca ccctggcaaa
    21421 tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg aatacgatac
    21481 tgctatcgcc agcatcgcca aacgtgcaga tgaagtgtac ttcctgaaag aaggtaattt
    21541 tgaagcagat aaaaacacga aagacgaagc gttactggat atgaccgctc aagtgctggc
    21601 gcaagagcac tcgcaaccgc agctgattgt tctgcatctg atgggctcgc atccgcaggc
    21661 ctgcgacagg acacaaggca aatacgaaac ctttgtgcaa tcgaaagaaa cgtcgtgcta
    21721 tctctatacc atgacgcaaa cggacgattt actgcgcaag ctgtacgatc agttacgcaa
    21781 cagcggcagc agcttctctc tggtttactt ttctgaccac ggtctggcct ttaaagagcg
    21841 cggcaaagac gtgcaatacc ttgcccatga tgataaatat cagcaaaatt tccaggtgcc
    21901 ttttatggtc atttccagcg acgataaagc gcatcgggtg attaaagccc gccgctcagc
    21961 caatgacttc ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg aaataaatat
    22021 caaatacccg tttatatctg agaagaaagc cgggccgata tacatcacca acttccagtt
    22081 acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac cttaaaaaca
    22141 aaaatccgcc ccgagaggcg gattttttat atcaccaaag tgattagaag cggtaaccaa
    22201 caccggcaat ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg taagagaagt
    22261 ccagagcaac gttttccatc gggttgaact gcagaccagc accgtaggag aaaccgtagt
    22321 cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg gaatttacca taacccacac
    22381 ccactacacc gtagatgctt gcccagtcgt taatgcggta agccggacca gcagtgatgc
    22441 cgtagtactg gtttttgttg tagtcaccag agcttgcagt acgacttttc tcggtgtaag
    22501 tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc atagcggtat ttcaggttga
    22561 aaccgcccat tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg ccagttacgg
    22621 tagaagtcgc agctacggaa gtacctgcgg tgaaagccag aactgcggcc agtgctgaaa
    22681 gacatgcaat ttttttcata accacctcaa atgtgattca aataagtcct aagttttaaa
    22741 tatatcaaaa attaatggga aactcttcgc gatttgtgat gtctaacggg ccatttcatg
    22801 taacagaacg tttccataca ccgctatcca tctaaattta aatcactttt tcagagaact
    22861 gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga ttattcctaa tcaccagact
    22921 aatgattcca tcaatcctgg cgcattttag tcaaaacggg ggaaaatttt ttcaacaaat
    22981 gctcaaccag cattgggtat atccagtaca ctccacgctt tacttaagtc tagatatttg
    23041 tgggagaaag gatgcctggt tcattacgta aaatgccggt ctggttacca atagtcatat
    23101 tgctcgttgc catggcgtct attcagggtg gagcctcgtt agctaagtca ctttttcctc
    23161 tggtgggcgc accgggtgtc actgcgctgc gtctggcatt aggaacgctg atcctcatcg
    23221 cgttctttaa gccatggcga ctgcgctttg ccaaagagca acggttaccg ctgttgtttt
    23281 acggcgtttc gctgggtggg atgaattatc ttttttatct ttctattcag acagtaccgc
    23341 tgggtattgc ggtggcgctg gagttcaccg gaccactggc ggtggcgctg ttctcttctc
    23401 gtcgcccggt agatttcgtc tgggttgtgc tggcggttct tggtctgtgg ttcctgctac
    23461 cgctggggca agacgtttcc catgtcgatt taaccggctg tgcgctggca ctgggggccg
    23521 gggcttgttg ggctatttac attttaagtg ggcaacgcgc aggagcggaa catggccctg
    23581 cgacggtggc aattggttcg ttgattgcag cgttaatttt cgtgccaatt ggagcgcttc
    23641 aggctggtga agcactctgg cactggtcgg ttattccatt gggtctggct gtcgctattc
    23701 tctcgaccgc tctgccttat tcgctggaaa tgattgccct cacccgtttg ccaacacgga
    23761 catttggtac gctgatgagc atggaaccgg cgctggctgc cgtttccggg atgattttcc
    23821 tcggagaaac actgacaccc atacagctgc tggcgctcgg cgctatcatc gccgcttcaa
    23881 tggggtctac gctgacagta cgcaaagaga gcaaaataaa agaattagac attaattaaa
    23941 tttacatttc tgcatggtta tgcataacca tgcagaattt ctcgctactt ttcctctaca
    24001 ccgtctttat atatcgaatt atgcaaaagc atatttattc cgaaaattcc tggcgagcag
    24061 ataaataaga attgttctta tcaatatatc taactcattg aatctttatt agttttgttt
    24121 ttcacgcttg ttaccactat tagtgtgata ggaacagcca gaatagcgga acacatagcc
    24181 ggtgctatac ttagtctcgt taattactgg gacataacat caagaggata tgaaattatg
    24241 agtaccgcta aattagttaa atcaaaagcg accaatctgc tttatacccg caacgatgtc
    24301 tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaggttat ccagtttatt
    24361 gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa cttcattgcc
    24421 gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga taccatggca
    24481 gaacgtgcag tgcagctggg cggtgtagct ctggggacca ctcaagttat caacagcaaa
    24541 accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct gaaagaactg
    24601 gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga agcgaaagat
    24661 gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt cctgtggttt
    24721 atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt acccgataaa
    24781 agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca cttatttagt
    24841 gcatccatct gctatctcca gctgattaag taaatttttt gtatccacat catcacacaa
    24901 tcgttacata aagattgttt tttcatcagg ttttacgcta aataatcact gtgttgagtg
    24961 cacaatttta gcgcaccaga ttggtgcccc agaatggtgc atcttcaggg tattgcccta
    25021 taaatcgtgc atcacgtttt tgccgcatct ctaaaaatca atgagttgca aaactggcac
    25081 gattttttca tatatgtgaa tgtcacgcag gggatcgtcc cgtggataga aaaaaggaaa
    25141 tgctatgaag tctgtattaa aagtttcact ggctgcactg accctggttt ttgcggtttc
    25201 ttctcatgcc gcggataaaa aattagttgt cgcgacggat accgccttcg ttccgtttga
    25261 atttaaacag ggcgataaat atgtgggctt tgacgttgat ctgtgggctg ccatcgctaa
    25321 agagctgaag ctggattacg aactgaagcc gatggatttc agtgggatca ttccggcact
    25381 gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc atcaccgacg agcgtaaaaa
    25441 agcgatcgat ttctctgacg gctactacaa aagcggcctg ttagtgatgg tgaaagctaa
    25501 caataacgat gtgaaaagcg tgaaagatct cgacgggaaa gtggttgctg tgaagagcgg
    25561 tactggctcc gttgattacg cgaaagcaaa catcaaaact aaagatctgc gtcagttccc
    25621 gaacatcgat aacgcctata tggaactggg caccaaccgc gcagacgccg ttctgcacga
    25681 tacgccaaac attctgtact tcatcaaaac cgccggtaac ggtcagttca aagcggtagg
    25741 tgactctctg gaagcgcagc aatacggtat tgcgttcccg aaaggtagcg acgagctgcg
    25801 tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac ggaacttaca acgaaatcta
    25861 caaaaaatgg ttcggtactg aaccgaaata ataacgctac acctgtaaag cgcactggca
    25921 gttccctctc ccctctgggg agaggattag ggtgaggggc gcaaacccgc tccggggcca
    25981 ttaattaccc tgaatttgat tatttacacc acggtaacag gaacaacata tgcagtttga
    26041 ctggagtgcc atctggcctg ccattccgct tctgattgaa ggtgccaaaa tgaccctgtg
    26101 gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga ttgctggcag gttttgcacg
    26161 cac
//
LOCUS       DAJTYD010000040        26127 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTYD010000040 DAJTYD010000000
VERSION     DAJTYD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26127
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.040"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(240..1013)
                     /gene="hisP"
                     /locus_tag="OO585_001516"
     CDS             complement(240..1013)
                     /gene="hisP"
                     /locus_tag="OO585_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6629145.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1021..1737)
                     /locus_tag="OO585_001517"
     CDS             complement(1021..1737)
                     /locus_tag="OO585_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6629146.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(1734..2420)
                     /gene="hisQ"
                     /locus_tag="OO585_001518"
     CDS             complement(1734..2420)
                     /gene="hisQ"
                     /locus_tag="OO585_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6629147.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2510..3292)
                     /gene="hisJ"
                     /locus_tag="OO585_001519"
     CDS             complement(2510..3292)
                     /gene="hisJ"
                     /locus_tag="OO585_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6629148.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3513..4295)
                     /gene="argT"
                     /locus_tag="OO585_001520"
     CDS             complement(3513..4295)
                     /gene="argT"
                     /locus_tag="OO585_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6629149.1"
                     /translation="MKKSILALSLLVGLSVAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4561..5130)
                     /gene="ubiX"
                     /locus_tag="OO585_001521"
     CDS             complement(4561..5130)
                     /gene="ubiX"
                     /locus_tag="OO585_001521"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6629150.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAISLPEDLFARWQGA"
     gene            complement(5225..6742)
                     /gene="purF"
                     /locus_tag="OO585_001522"
     CDS             complement(5225..6742)
                     /gene="purF"
                     /locus_tag="OO585_001522"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6629151.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(6779..7267)
                     /gene="cvpA"
                     /locus_tag="OO585_001523"
     CDS             complement(6779..7267)
                     /gene="cvpA"
                     /locus_tag="OO585_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6629152.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7526..8188)
                     /gene="dedD"
                     /locus_tag="OO585_001524"
     CDS             complement(7526..8188)
                     /gene="dedD"
                     /locus_tag="OO585_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6629153.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8178..9446)
                     /gene="folC"
                     /locus_tag="OO585_001525"
     CDS             complement(8178..9446)
                     /gene="folC"
                     /locus_tag="OO585_001525"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6629154.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9516..10430)
                     /gene="accD"
                     /locus_tag="OO585_001526"
     CDS             complement(9516..10430)
                     /gene="accD"
                     /locus_tag="OO585_001526"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6629155.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGSIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10586..11245)
                     /locus_tag="OO585_001527"
     CDS             complement(10586..11245)
                     /locus_tag="OO585_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6629156.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11328..12140)
                     /gene="truA"
                     /locus_tag="OO585_001528"
     CDS             complement(11328..12140)
                     /gene="truA"
                     /locus_tag="OO585_001528"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6629157.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12140..13153)
                     /locus_tag="OO585_001529"
     CDS             complement(12140..13153)
                     /locus_tag="OO585_001529"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6629158.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13219..14355)
                     /gene="pdxB"
                     /locus_tag="OO585_001530"
     CDS             complement(13219..14355)
                     /gene="pdxB"
                     /locus_tag="OO585_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6629159.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14454..15449
                     /gene="flk"
                     /locus_tag="OO585_001531"
     CDS             14454..15449
                     /gene="flk"
                     /locus_tag="OO585_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6629160.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15446..16624)
                     /locus_tag="OO585_001532"
     CDS             complement(15446..16624)
                     /locus_tag="OO585_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6629161.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16944..18164)
                     /gene="fabB"
                     /locus_tag="OO585_001533"
     CDS             complement(16944..18164)
                     /gene="fabB"
                     /locus_tag="OO585_001533"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6629162.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18323..20329
                     /gene="mnmC"
                     /locus_tag="OO585_001534"
     CDS             18323..20329
                     /gene="mnmC"
                     /locus_tag="OO585_001534"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6629163.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQSFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20450..20728)
                     /locus_tag="OO585_001535"
     CDS             complement(20450..20728)
                     /locus_tag="OO585_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6629164.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(20762..21310)
                     /gene="epmC"
                     /locus_tag="OO585_001536"
     CDS             complement(20762..21310)
                     /gene="epmC"
                     /locus_tag="OO585_001536"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6629165.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21310..22119)
                     /locus_tag="OO585_001537"
     CDS             complement(21310..22119)
                     /locus_tag="OO585_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6629166.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22119..22943)
                     /gene="mepA"
                     /locus_tag="OO585_001538"
     CDS             complement(22119..22943)
                     /gene="mepA"
                     /locus_tag="OO585_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6629167.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(22947..24032)
                     /gene="aroC"
                     /locus_tag="OO585_001539"
     CDS             complement(22947..24032)
                     /gene="aroC"
                     /locus_tag="OO585_001539"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6629168.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24067..24999)
                     /gene="prmB"
                     /locus_tag="OO585_001540"
     CDS             complement(24067..24999)
                     /gene="prmB"
                     /locus_tag="OO585_001540"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6629169.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25165..25716
                     /gene="smrB"
                     /locus_tag="OO585_001541"
     CDS             25165..25716
                     /gene="smrB"
                     /locus_tag="OO585_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6629170.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(25789..>26127)
                     /locus_tag="OO585_001542"
     CDS             complement(25789..>26127)
                     /locus_tag="OO585_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6629171.1"
                     /translation="DVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNF
                     DERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDST
                     CRFTYILNMY"
BASE COUNT         6407 a         7169 c         6620 g         5931 t
ORIGIN      
        1 taaataccgc atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct
       61 caaatttaag atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta
      121 tctttggaca gcgaattcag aagtattttt agcggactgc aacttttaca tttcagcgga
      181 agctgactcg caaattcccc atgaaaatac tccggcagcg ttagccaccg gagaggaaat
      241 tatttcagcg atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac
      301 tgctccggcg cgccctcttc ttctattttc ccctgatgga ggaaaatgac atgagtagaa
      361 acatggcgag caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc
      421 agttgctgca taatacgcaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt
      481 tcatcaaaca gtaaaacttc cggttccatc gccagcgccc gcgcgataga aacacgctgt
      541 tgctgaccgc cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact
      601 tttgccagat acttgaccgc ccgctcgtgc gcttcctgct tgctcaggcc caacacctga
      661 atcggcgctt ccatgacgtt ttccagcacc gtcatatggc tccagagatt gaagtgctgg
      721 aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat ttttatcggc gactttgagt
      781 tgaccgtctt tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgacccttca
      841 ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat
      901 ccgatgatgc ttattacatc tccggcattc gcttgcagtg agaccccttt cagcacttca
      961 tgttcgccgt agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc
     1021 tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag
     1081 gctgatcagg acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa
     1141 aggttgatac gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc
     1201 cgtaaacgcc agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa
     1261 cgcaatacgc agcgcagaag gcaaaataat gcaacgatac attttaaaag tcgagaagcc
     1321 ataggctctg gcggcttcaa tttccccatg cagaaccgaa cggattgccc cagcaaaaat
     1381 ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc acggtacagt tcaggccact
     1441 gcggaagaaa gcgttaagga attcggttcc cttaacaatc tcaagcgtgt atatgccgga
     1501 atagaacacc agcaactgaa catacagcgg cgtaccgcga aaaatatagg taaataacca
     1561 gattggaaac tggatgtatt tattactgga gacacgacca atcgccagaa acagcgccag
     1621 gactccgcct atcactaccg acaaaataag cagccacaga gtgatcgcca caccagtaaa
     1681 gcgataaccg tcggtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg
     1741 tcagccctct tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa
     1801 acagtggtga aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag
     1861 gtacttttgc ctgccagttg ggtggctttg accacatctt ccaggccgag taacgaaacc
     1921 agtgcggtag atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc
     1981 atcatcgccg gaaacatgat ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc
     2041 gccgcctcta tatgtccttt cggcactgcc ataaaagcac cacggaacgt ttcggtaaaa
     2101 taagcaccgt agataaaacc gagagtgata ataccagcga ccatcggatc gatatcaatc
     2161 tgcccgacgc ccatcgcctc cgtcaccgtg tttagcgcaa tctgcaaacc gaagaaaatc
     2221 agcagcatca acactaagtc cggcacgcca cgaatcagcg tggtgtaccc ttcgaaaatc
     2281 agccccgaaa gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca
     2341 gcgagcacta cagagctgat agccagctcc agcgtgacga gcgcaccctg taaaataaca
     2401 cctgaaaacc catataacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg
     2461 tctccctccg gttacccgaa gggagcgggc tttctttcac cgatggcgat tagccaccat
     2521 aaacatcaaa atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt
     2581 cggcaaaggc tttgttcagt gcttcgcgca gttcgttatc ttctttacgc aggcccatgc
     2641 cggtccctac gccaaacagt ttttcatctt taacagacgg gccaccgaat ttgtaatctt
     2701 taccgacagg ttgtttgagg aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat
     2761 caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt
     2821 caatgccttt tggtgcccaa tgttcattac cgaacgtctc ctgggtggtg ccctgcaata
     2881 cgcctacccg tttgcctttc agcgactcga ctgtcggctg aatgtcagaa tttttcgcca
     2941 ccaccaaacg agaatcggca gcgtacagtt tgtcggtgaa ggctatttct tgctgacgtt
     3001 tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga
     3061 ttaacgcatc cagcggattt tcgacaaacg tacattgcgt attgatgcgt ttgcataatt
     3121 cctttgccag atcgatatcg aagccaacca gttcgccttg tgaatttttt gattcaaatg
     3181 gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg aatcgcagca aatgccgcag
     3241 ttgcgctgga gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct
     3301 caactgacag tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa
     3361 attgaccatc aaggcaaaat atcccgcccc gttagatgta ttatttttca ttaaaaatgc
     3421 ttaagtatgc atttcaatgc accacgatag tgcaacaaac ctagcgtgca ccatgatgat
     3481 cattccatca ggtacagctt cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa
     3541 tactttttcg ccatcttgtc gtaggtgccg tcctgacgca gctcgccaag cgccttattg
     3601 aaggcagccg tcagttcagc atcatcttta cgtagcccta caccggtgcc atcaccgaag
     3661 tattttttgt cttttactga tgagccagca aaggcgaaat ctttaccagc aggttgcttg
     3721 aggaatcctt cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc
     3781 agatcggaat agaccaaatc ctggttggca taggccacca catccacgcc tttgctacgc
     3841 caggtctcgt tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct
     3901 ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg cagcaatcaa acgagaatct
     3961 gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac gtttatcggt aatggaaagc
     4021 gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca
     4081 ctggcaaccc aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata
     4141 tcaaagccaa caaaatcacc tttagcatct ttcgacgaga acggcgcata ggtggtatcg
     4201 gttccgatac gtaccgtctc cggtagcgcc gcatagctgg aagccgctac ggagagaccg
     4261 actaacaaag acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct
     4321 tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat
     4381 cttgacgaca acagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtcc
     4441 tgaagttgaa gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa
     4501 caaaatgtcg aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag
     4561 ttatgcgccc tgccagcggg caaagagatc ttcaggaagg cttattgcaa actggtcaag
     4621 aacacgatta accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc
     4681 cggaacagga ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg
     4741 gcctaagtgc aatggtgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac
     4801 cacatctgcc gcacgggtca gtaagccatc agtatagcta tggacaatgc cggaaagggt
     4861 tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg aaagaaccgg aagagatgct
     4921 ggcggaaata tcgcgcgcat cgtgcgtgac atcagctaat gcctgcactt cgcgcaggga
     4981 aaaatccgtt tcgagggata aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc
     5041 gatatctgtg acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc
     5101 accgctgatg cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag
     5161 actttgcagt atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctcagcgaa
     5221 ggcatcatcc ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcactgctt
     5281 tggcgtcatc attacgtaac gtatcgagga aatcgaggta gtcctgatca acatctttgg
     5341 tgacgtagac gccgttgaac accgagcatt caaactgctg gatatccgga ttttcagcgc
     5401 gaacggcgtc gatcagatcg ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct
     5461 ggcgaatttc atcaacttcg cgaccgtgag cgatcagttc cgtggcgctc ggcatatcaa
     5521 taccataaac gttcgggaag cgaatttccg gggccgcaga agcgaggtac actttcttcg
     5581 ctccggcttc gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt
     5641 cgtcgaccag caggacgttt ttatcgcgga actcggcgcg gttggcgttc agtttacggc
     5701 gcacggactt acgacgcagc tgctggcccg gcatgatgaa ggtgcggcca acatagcggt
     5761 ttttaacgaa gccctggcgg tacggcttgc ccagaatacg agcaatttcc agcgcgatat
     5821 cacacgaggt ttccgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg
     5881 caattttctc acccagtttc gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt
     5941 cgataaacga gtccgggcgg gcaaagtata catactcaaa caggcacgga ttgctgaccg
     6001 gattgtcagc acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc
     6061 ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag
     6121 cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa
     6181 tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat aatcatcgcc acacaggcat
     6241 atgcgccgcg gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca
     6301 gcgggtagtg gcggaagttg tccagctcgc tagcgaagat attaagcaga atttccgagt
     6361 cggaagtggt gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag
     6421 cgttggtcag attgccgttg tgggcaagcg taatgccata cggggagtta acgtaaaacg
     6481 gctgcgcttc agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca
     6541 tattgccctg caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg
     6601 ctttacgcaa acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac
     6661 cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg
     6721 cgataccgac aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt
     6781 acgctctggg caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa
     6841 aactgaactg cgggatcagc tgtgatttgc tccagtcttc gcttttcgac accccggtaa
     6901 aggagtcgag aaagaagaga atggcggcaa caatcaacac accgcgcaac gcaccgaaac
     6961 agacgcccag cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca
     7021 cgaagttcac gatagcacca acgatcaggg tagcgataaa cagtaccgcg atggcaattc
     7081 catttcgaac cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat
     7141 gactggcaac aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc
     7201 cgcggatcag gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc
     7261 agaccatatg tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag
     7321 aaaaagaaaa cgtttgcgta gggatttcct tcccacgcat caataaaaat ggcgctgaaa
     7381 aaatattcaa cgccatcgac tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg
     7441 cggcgtaaac gccttatccg acctacggtt ctaacactgc ccaagcctga taagacgcgc
     7501 cagcgtcgca tcaggcaaga ctgtattaat tcggcgtata gcccattacc acgccactta
     7561 agccagaaag ttgcttcaac tcacccagcg aacctttcag cttatctttc gaggcatccg
     7621 gcccaaccag aatacgggta attttaccct gcactggcgt ggatggcgac gtataaaccc
     7681 gataaccggc accgcgcagc ttaccgacaa tctcattcac tttatcggca tttttcagcg
     7741 cacccagttg cacaacatag gctttacccg ttggcgctgc tttttcttcc acgaccggct
     7801 tcggttctgg cttcggcgca ggtggtgctt caaccttcgg tttaggcggc tccaccggtt
     7861 tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa
     7921 tagtggccgg atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc
     7981 cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg catcatatca ggctcatcgc
     8041 gatcgcccgc ttttggaacc agcgggatag ccgcaaactc atcctgataa tgttttttct
     8101 gcccgtccag cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca
     8161 ctaaccgatt ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca
     8221 tgtgcgaccg tgtggaaaga accacacacc agcacggtgt cttccgcttt agcgtccgcc
     8281 attgcggcat cccatgcctg cgcaacgcta tcaaatgatt tgccgttacc caaatgctca
     8341 agcagttgtt ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca
     8401 accacgcttt tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata
     8461 accgccagca cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc
     8521 gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa
     8581 cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacttccagc
     8641 ccgctggcac gcagtgccgc cagcgctgtt gcggcattcg gttgcgggac aagcggcaac
     8701 ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa
     8761 tagttccact caacgccccg acgttgtaac agtgcacctt tttcctgcgc cacatcagca
     8821 atggtagaag gcatttccgg ctcaccgaca attgccggtt ttgcgctgcg aaagatgcct
     8881 gctttctcgc gaccaatact ttcgcgatct ggccccagcc agtcggtatg atccagcgca
     8941 atactggtta ctaccgcgac atcggcatcg acaatattgg ttgcgtccag acgaccgccc
     9001 agccctactt ccagaatcac cacgtcaagt tgtgcctgtt tgaacagcca caacgccgac
     9061 agcgtaccgt actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca
     9121 aaagaggcgg tgtgggccga ttccggcaat tcctgaccct gcacacgtac gcgctcggta
     9181 taacgcacca gatgcggcga actgtagacg cccactttgt accctgccgc catcagaatc
     9241 gactccagcg tacggcaggt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat
     9301 ggcgctggtt tcagaacgcc aagacgagcc gcgacctggc tcacgcgctc aaggccgaga
     9361 tcgatagttt tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg
     9421 gcttgaggag tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa
     9481 aagggcagag ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatccggt
     9541 accgggggta ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc
     9601 aacttcgcca gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg
     9661 atcgagcctt tctcgatcag gaattcactg cgctggaatc ctagcggcag tttttcgcga
     9721 acggtctgtt cgataacacg cggaccggca aagccaatta acgctttcgg ttcagcgatg
     9781 ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc
     9841 acggagatgt acggcaagcc gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc
     9901 atctgcatca gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag
     9961 atcagcgggc agttatcttc cagcgcctgc tcaacggcac gcacgaaacg tgcacccaca
    10021 acagacccca ttgaaccgcc cataaaggcg aactcgaatg ccgcagcgac aaccggcatt
    10081 ccatacagag tgcctttcat caccaccagc gcatcttttt cgccggtttc tttctgcgca
    10141 gaagccagac ggtctttata cttcttggag tcacgaaact tcagcacatc tttcggctca
    10201 agctcgctac ccatctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc
    10261 gctgtcatac gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca
    10321 gcgcggtata aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc
    10381 gccttgcggg tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt
    10441 tctgtctgaa cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc
    10501 ccccaacaga ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa
    10561 agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac
    10621 gaataaattc gaaaacccca ggcaatattg aaacaaaaat aatgccaaca atcatcagct
    10681 taaggttgct ctgaatgaag gggagcatgc cgaagaaata acccgcgtag gtaaaaatca
    10741 gtacccacaa cagtgcgccg atcacgttat aagcggcgaa atgacggtac gacatgtggc
    10801 ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg tacaaaacgg gcgagaataa
    10861 tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt atcaagataa ctgcaacgaa
    10921 agattttcga atccgggtta ctgaacagtt tgtcaccaaa taaccgtcca atggtatagt
    10981 tcactgcatc accgacaata gcggcaatca acataagcac caccatcata tggacattga
    11041 gatcgttagt ttccagcgac gccaatgcac cagcaacaaa cagcagcgaa tcaccaggca
    11101 ggaatggcgt taccaccagc cccgtttcgc agaacagaat caaaaacaga atggcataaa
    11161 cccagacgcc gtactccgcg accagctctg ccagatgcac gtcaatgtgc aggataaaat
    11221 cgatgaggaa ataaatcagg tccatatttg cctatgcctt gtactcgtca tctttcaggc
    11281 tgtaactacg ttggctacag cctgaaatgt tccgagcatt attctcgtta gtccgccaga
    11341 aatagcgggc ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc
    11401 agatacagcc cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc
    11461 aactctgcta tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca
    11521 atattcctga ccatatgatg tacaaaggca ttcgctttga tatctaccac cacataagga
    11581 ccgtgacgcg tgacattaat atgcataacg ttgcgccacg gggttcggga ctggcactgc
    11641 accgcacgga acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc
    11701 cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc
    11761 agccgatgat tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga
    11821 aaatcatcag gtacagcttt aacccaacgc acggcgatgt catcaggtaa attcgcattt
    11881 acgcccaagg tccacgccgc gtctttgcgc tgcgcggttg tttcgaaatg caccacctgc
    11941 ccggtaccgt gtacccctgc gtcagtacgc ccggcgcaga agacggtgat gggttcgttc
    12001 gccacctggg agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt
    12061 tgccagccgt aatacttact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc
    12121 agttgttgct ggtcggacat tagtacagat actcctgcac cagtttctcg gcgattttta
    12181 ctgccatcag cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tggacttgct
    12241 ccggcatacc gtagtcatta cgcacgcagc caacagaaag atgcggagta cccgaagcat
    12301 cccctacctg agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa
    12361 acgcatcacg cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg
    12421 catgaccgta gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct
    12481 gcaggatttt gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat
    12541 ccggcaacaa cggcagcata ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa
    12601 tcggaatgcc gttgagcaat ttcgcactct gccccgctaa cgcatcgacc gcttttttgc
    12661 cctgggcgga ggctgaaatc aggctggtaa cactgatacg cgacaaaccg ccctgatcga
    12721 ttaacggttt cagtgccgcc agcagctggc tggtcagact gtctggtacg gcgatgacat
    12781 tccggttgcg gtaatcggtc agtacaaacg ggtttacttc cggcaccacc agcggtacgt
    12841 cgggttcgag agcaaacagt ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt
    12901 cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg
    12961 tccagtcgaa ttcagcggca tcctgcacgg tgattgtttt accaccaaag cgcagttgtt
    13021 cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag
    13081 ccagcgtttc aagcagggct tcgcccacag cgccagttgc gcccaggacg gcaatgttcc
    13141 agccttcaga catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca
    13201 gagagcatga agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca
    13261 cagcaatgat gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg
    13321 ctcaagatag tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag
    13381 cggtgcgtca tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg
    13441 atcgagcggg ccatgcagcg taatgcgacc aaactctggc gcgggcagta atgtatccag
    13501 cgcaacgtgc tgttcatgcc caataaactt gctataagct tcaaacactt gcgtagtacc
    13561 gcgtgcttta ccttccaggg tataacctgc gatatgcggc gtgccgatat ccacttttgt
    13621 cagcaactcc acgttaagtt ccggttcgcc ttcccagaca tccagcacta cgcttaactt
    13681 ctggccttca ttcaggcagg tcagcaacgc agtattatcg acgactgcgc cacggcaggc
    13741 gttaatcaga atcgctcccg gcttcaggct acggatcagt ttttcatccg ccagatgcag
    13801 cgttttgtac ggcccatctt tgaagagtgg cgtatggaaa gtcagaatat ccgcatgctg
    13861 aactaattca tccagcgagc ggaaatcacc ctcatcccca cggtcagcgc gaggcggatc
    13921 gcaaagcaat gttttaatcc ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt
    13981 accaacgccc acgatcccca ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag
    14041 catcagtagt gaggagaaaa catattccac caccgcaatc gcattacagc caggtgcagc
    14101 ggaaaaacca attcccgcct gcttcaacca tgcttcatcg acatggtcgg tccccgcagt
    14161 ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac
    14221 cgaacgcacc atcagcgcat ccgcgtctgc cagttgagcg acggcgattg gacgcccggg
    14281 aaccgcggtc acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc
    14341 aacaaggatt ttcacgtttg tgttacctgt atgagacgag agttaaccgg acaagtgtgc
    14401 cataatctcg cggccaggca tacttgcgaa gatttcaggt ataaggatac gtaatgatac
    14461 aacctatttc cggccctcct cctgggcaac caccaggtca gggagataac ctgccgtctg
    14521 gcgcgggcaa tcagccttta tccagtcagc aacgtacttc gctggaaagc ttaatgacga
    14581 aagtgacctc actgacgcaa cagcaaagag cagaactgtg ggcgggtatc aggcacgata
    14641 ttggtctgtc gggagattca ccgctgcttt cgcgtcactt ccctgccgct gagcataatc
    14701 tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat
    14761 taggggagta tttacgtctg gggaataatc gtcaggcggt cacggattat atccgtcata
    14821 actttggtca gacgccgctg aatcagctct caccggagca attaaaaacc attctcaccc
    14881 tgttgcagga agggaagatg gttattccgc aaccgcagca gcgcgaggcg accgaccgtc
    14941 ctttattacc ggcggagcac aatgcgctaa aacagctggt gaccaaactt gcggcggcaa
    15001 cgggggaacc cagcaaacag atctggcaat cgatgctgga actttccggg gtgaaagatg
    15061 gcgagttaat tccagcgaaa ctgtttaacc atctggtgac ctggctacag gcgcgtcaga
    15121 cgctaagcca gcaaaatacg ccgacgctgg aatcactaca gatggcgcta aaacaacctt
    15181 tagatgccag tgaactggcg gcgttatcgg catatatcca gcaaaaatat ggcctttctg
    15241 cgcaatcatc gctttcttct gcccaggccg aggatattct taatcagctt tatcaacggc
    15301 gggttaaagg gattgatccg cgtgatatgc aaccgctgct taatcctttt ccaccgatga
    15361 tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta
    15421 tcctgatgct ggtctggctg gttcgttaac cccgacgaaa tgacagtatc gtgacaataa
    15481 tacccagcac cgcagagatc gccccggcaa gaaataccga agagtaacca aacgtggtcg
    15541 ccagcattcc cgccagcggc ccggagacgc caagggcgat atcctgaaac gcggcgtaac
    15601 cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt aaccacctcc acgcccagcg
    15661 caggaaagat aagcgaacat ccggctccgg ttaacgccgc gcccgctaat gcgacccatg
    15721 cacctggggc ttgccagagc agcaacaagc ccaccgtttc tacaagcaga gagacaatcg
    15781 ccactttcac gccgccaaaa cggtccggca tccagccaaa catgacgcgc atcacgacaa
    15841 atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt
    15901 agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc
    15961 ctggtttcca gataagcccg acaacgctcc acagcgatgg acgttctccc gccagggccg
    16021 gtactttgcg cactgtgccg ttacaggccc acgccagtaa gggtaatacc attgtggtga
    16081 tcgccagtgc ggcaaaaccg taatggctat gaatcaacag gccaagcggc gcaccaacag
    16141 cgagggcacc gtaaatcgcc attccgttcc atgacatcac tttgccagag tgttttgccc
    16201 ctacgatgcc taatccccag gtcagagcgc ctgtcagtaa ctggctttca ccaaagccaa
    16261 gaatcaaacg cccgacgacc aacagggcaa atttcaacgg tgcggagaca ggcaaaatcg
    16321 ccgccagcag caacgcgccg ccagccagac cacaagctaa catcccctga agcgccgaac
    16381 gttttgcacc atattgatcg gccagtcgcc cggcatagcc acgcgtcagc accgtagcca
    16441 gaaactgaat cccgacggca atcccgacca tggtattgcc atagcccaga tcatgatgaa
    16501 caaacagcgg gataaccggc aacggcagcc ctacggtcat gtaggtgaga aaaaccgcaa
    16561 aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt ttcggtttgg cttacagcag
    16621 tcatgcatta ctccagaatg cagcgcaagg cgaggagtat ccccgtctca tctctctggt
    16681 ttcagggtta cagtgcgttg gcaggattta acgcgtacgt cttttcagaa ggaaatcgac
    16741 aaagcgggaa gtttgcctgg aactggcggc gattgtcaat gatgtgaaaa agggaaccat
    16801 caggttccct tttgcgttag tgccggaggc cgcattggcg cgtaacgtcg gatgcgacgc
    16861 tggcgcgtct tatccgacct actccgacta ctccgactac tccgactact ccgactactc
    16921 cgactactcc gacctactgc gaattaatct ttcagcttgc gcattaccag cgtggcgttg
    16981 gtgccgccga agccgaagct gttagacata acggtggtca gttcgcgatc ggtcgtttcg
    17041 gtcacgatgt tcagacctgc cgcctgctcg tccagctctt caatgttgat gctcggggcg
    17101 ataaagccgt gttccagcat cagcagagag tagatagctt cctgtacgcc agcagcgccc
    17161 agagagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc gccgaacact
    17221 tcacggatag ctgccagttc tttcacgtcg ccaaccggag tcgaagtacc atgagagttc
    17281 aggtaatcga ttggggtatc aacgccgtgc atcgccatct tcatgcagcg tactgcgcct
    17341 tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc aacgatttca
    17401 gcatagatgt gagcaccacg cgccagcgcg tgttccagct cttcaaccac taccataccg
    17461 ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt aagtacggga ggctttttcc
    17521 ggggtgtcgt tgtatttagt agacagcgca cccatcgcgt cgaattcgca ggccatttcc
    17581 cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag ttggatctgc
    17641 tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat ggagtagtta
    17701 acgccatgaa ttttaaacgg cgtggcgagg caggcagaaa cgccggatgc catcgctttg
    17761 gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc gtcagcgccg
    17821 aatacctgga aacgcgggga accgccgcca gaacctgcaa tcaggccaac gcgcgggtta
    17881 ttctggtaag cttccggaga aaggcccgca tctgcgattg cctgctccat agaaaggaat
    17941 gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag gccagtggta
    18001 tccagtttta cgttgcccca gacgtggcta cgcatgccgg aatccttaag ctcctgagag
    18061 aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg gttattaccg
    18121 atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata cctctgtaag
    18181 tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg aacaagtccg
    18241 atcagccatt taatagagaa atttgcgcag ccttacacac atcgctaaga tcgagccacc
    18301 gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca acctcgaatt
    18361 taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt ccaacgataa
    18421 cgggctggaa gagacgcgtt atgtttttct gggaggcaac caattagagg tacgctttcc
    18481 tgagcatcca catcctctgt ttgtggtagc agagagcggc ttcggcaccg gattaaactt
    18541 cctgacgcta tggcagtcat ttgatcagtt tcgcgaagcg catccgcaag cgcaattaca
    18601 acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt tagccttagc
    18661 gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc agtggccaat
    18721 gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc tggatttatg
    18781 gtttggcgat attaacgaac tgaccagcca acaggacgat tcgctaaatc aaaaagtaga
    18841 tgcctggttt ctggacggct ttgcgccagc gaaaaacccg gatatgtgga cgcaaaatct
    18901 gtttaacgcc atggcaaggt tggcgcgtcc gggcggcacg ctggcgacat ttacgtctgc
    18961 cggttttgtc cgccgcggtt tgcaggacgc cggattcacg atgcaaaaac gtaagggctt
    19021 tgggcgcaaa cgggaaatgc tttgcggggt gatggaacag acattaccgc tcccctgctc
    19081 cgcgccgtgg tttaaccgca cgggcagcag caaacgggaa gcggcgatta tcggcggtgg
    19141 tattgcctgc gcgttgttgt cgctggcgct attacggcgc ggctggcagg taacgcttta
    19201 ttgcgcggat gaggcccccg cactgggtgc ttccggcaat cgccaggggg cgctgtatcc
    19261 gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt ttacttttgc
    19321 tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg gcgtcacgca
    19381 gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa tggatttacc
    19441 cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg gcgttgcgac
    19501 aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag aactgacccg
    19561 taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat atcagttaca
    19621 gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc agcaagcaac
    19681 acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgattcagcc aaacgtcgac
    19741 tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc cggaattggc
    19801 agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc cggcgaatca
    19861 acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt acagtgagga
    19921 cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac agtgggcaaa
    19981 agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca cccgcgatca
    20041 tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat atgcgtcgtt
    20101 ggcggagcag aaagatgagg cggtaagcgc gccggttttt gacgatctct ttatgtttgc
    20161 ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc tggcggcgca
    20221 gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa acccgaatcg
    20281 gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat ctgctctcgc
    20341 agtgtttgcc ggatgcggcg tgaacgcctt atccgaccta cgtgtgacgt gtaggcctga
    20401 taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tactgctgtg
    20461 acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca
    20521 attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag
    20581 cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat
    20641 acccactggc aaacaactca tcatcactgg catggtcaat catgccgtcg attaatgcca
    20701 gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcaccggc
    20761 ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat
    20821 aattttgtag tgctttaatg aagcgtgccg gacgctcggg aatgccattt gtcagataat
    20881 ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg aagtcgcctt
    20941 ccagattgtc gcaactgacg ttaaacggat atcccgccgc cacacagaac aaccagtcga
    21001 gtgcctgcgg cttcacttca acatcttcaa actggctttg cgtttgggca tcacgcccat
    21061 ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc
    21121 agtgcgaaat ctcatgaatt gcgctggcat aaaagccgtg ggcaaagaca attcggttat
    21181 acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg
    21241 tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctca tagtggtgtg
    21301 tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag
    21361 taatttggca ctcatcaccg ccgagacgat aacgatcatc gggcggatca gtttttgtcc
    21421 tttgctcagc accagtcttg aacccatgcg cgcgccgagg aactgcccca ccagcatgac
    21481 aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt
    21541 tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag
    21601 cgtaacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa
    21661 accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc
    21721 ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatta ccagaatggg
    21781 caaaatctgc cgtaagacat cagcctgaac gtattgcacc agcagtgcgc cgctcattga
    21841 gccggcaaag gtcatggcga tattgagttt ctgatcgctt aaactaacca ctttgcggcg
    21901 aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag
    21961 cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta acccaccgcc
    22021 accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa
    22081 cagcggggaa accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt
    22141 agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttcct
    22201 ggttttggag gtgcaaacca gctttgcagt tctgccccgc aaccatcgcc tggtggcggt
    22261 aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac atgcatatgc
    22321 gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca
    22381 aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc tttgtcctgg
    22441 gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac aacgtgttta
    22501 ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga
    22561 gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg
    22621 ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc
    22681 agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa
    22741 tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg caacgtgtca
    22801 gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt
    22861 tgggttattt tttgccacgg cgtcgctgcc aagctagcgc tactggcaag cagagccagc
    22921 agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca
    22981 ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg
    23041 atcggcactg cacggatccc gacacaggga tcgtgacgac ctttggtgat catctcaact
    23101 tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc
    23161 gccatatgag caatgatttg ctgcccgctg ctgataccgc cgagaatgcc gcccgcatgg
    23221 ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact
    23281 acgtcaaagc cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg
    23341 gcgatgtcgg catccaggcg gtcaaatacc ggctcgccaa gtccggcggg gacgccactg
    23401 gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt
    23461 tcatctaacg cgtcgatttt gtccggatcc gggcagaaga acggattttg ttcaaccagc
    23521 gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaagcagcc gcggatttca
    23581 ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg
    23641 gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg
    23701 taggtgtaat cagcatggcc tgggcggaaa acgtctttaa ttgcgctgta atcctgagag
    23761 cgctggtcag tgttttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa
    23821 acaccagaga gaattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc
    23881 ccagggcgac gacggtcgag gtcatgttgc aggtccgctt ccgtcagcag aatgcctggc
    23941 ggaacaccat cgacgatgca gccgagcgcc agcccatgcg attcgccgaa ggtggttacg
    24001 cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt
    24061 gtttacttaa tctttataaa tcgcgaaata ttcacgggcc gccagcagct gctctttggt
    24121 gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatccgg
    24181 atattgttcc ataagatgta ccatgctgtt gccgacttca caaatcaaca cgccatcatc
    24241 agcaaggtaa tctgccgcgt ttccaagaat gcgacgcgtc agtttaaggc cgtcggtgcc
    24301 agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc
    24361 cgcgtcgaca tacggcgggt tagtgacaat cagatcgtac tgcactttcg gcaagtcgcg
    24421 gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg
    24481 ttcagcaacc gccagcgcgt cgggagagat atccaccgcg tcgacttctg catccgggaa
    24541 ggcataagca caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg
    24601 cggttgtttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg
    24661 cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt
    24721 caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacgatg
    24781 tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca gcggcaggta
    24841 gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta
    24901 ccagatattt gccgcgctga agcggctcac tgaccagcgc aacatgtcct gaatggtttg
    24961 cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc
    25021 gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg
    25081 tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg
    25141 aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag
    25201 gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac
    25261 cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct
    25321 gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg
    25381 aaatatgttc gcccggatgt cagccatttc gaggcgaaga aactgcgccg cggcgattat
    25441 tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg
    25501 ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg
    25561 cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg
    25621 atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt
    25681 gaagtggaag agtggttgcc gccggagttg ccctgaggag ttgagcgtgt tgtgccagga
    25741 gcgcggaatg gcaggggcga atgaccctgc caggtgagaa aaggtgcgct aatacatatt
    25801 cagtatgtaa gtgaatcggc aggtagaatc tatgggctgt cgagatgtcg tgtccacgcg
    25861 tagcttcata tcatgcagtt gcctggacgt aatcggttgc ggctcaggcg tcaccgtcac
    25921 tttggcatta tcacagccgg aagtcgagaa ggtcatctgc tgtgcgataa aacggcagta
    25981 gacaccacgc tcatcaaaat tatttccgtt acacagtttg ccattttcca tcaggaaagt
    26041 gggcagtgtt tgctcaataa caccacctgt tgactgcaac ctgacgactt tgttatgtac
    26101 aacataattt tcattccagg aaacatc
//
LOCUS       DAJTYD010000041        25322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTYD010000041 DAJTYD010000000
VERSION     DAJTYD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.041"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..173
                     /locus_tag="OO585_001543"
     CDS             <1..173
                     /locus_tag="OO585_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR6629172.1"
                     /translation="ITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQ
                     LSPDAWDDVSQE"
     gene            243..1019
                     /gene="yaaA"
                     /locus_tag="OO585_001544"
     CDS             243..1019
                     /gene="yaaA"
                     /locus_tag="OO585_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6629173.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1172..1468)
                     /locus_tag="OO585_001545"
     CDS             complement(1172..1468)
                     /locus_tag="OO585_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6629174.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1682..2968)
                     /gene="thrC"
                     /locus_tag="OO585_001546"
     CDS             complement(1682..2968)
                     /gene="thrC"
                     /locus_tag="OO585_001546"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6629175.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLCLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2969..3901)
                     /gene="thrB"
                     /locus_tag="OO585_001547"
     CDS             complement(2969..3901)
                     /gene="thrB"
                     /locus_tag="OO585_001547"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6629176.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3903..6365)
                     /gene="thrA"
                     /locus_tag="OO585_001548"
     CDS             complement(3903..6365)
                     /gene="thrA"
                     /locus_tag="OO585_001548"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6629177.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFVQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6390..6509)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6445..6510)
                     /gene="thrL"
                     /locus_tag="OO585_001549"
     CDS             complement(6445..6510)
                     /gene="thrL"
                     /locus_tag="OO585_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6629178.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6724..7410)
                     /locus_tag="OO585_001550"
     CDS             complement(6724..7410)
                     /locus_tag="OO585_001550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6629179.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7810..7950)
                     /locus_tag="OO585_001551"
     CDS             complement(7810..7950)
                     /locus_tag="OO585_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629180.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8046..8762
                     /gene="arcA"
                     /locus_tag="OO585_001552"
     CDS             8046..8762
                     /gene="arcA"
                     /locus_tag="OO585_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6629181.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8822..10174)
                     /gene="creD"
                     /locus_tag="OO585_001553"
     CDS             complement(8822..10174)
                     /gene="creD"
                     /locus_tag="OO585_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6629182.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10232..11656)
                     /gene="creC"
                     /locus_tag="OO585_001554"
     CDS             complement(10232..11656)
                     /gene="creC"
                     /locus_tag="OO585_001554"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6629183.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11656..12345)
                     /gene="creB"
                     /locus_tag="OO585_001555"
     CDS             complement(11656..12345)
                     /gene="creB"
                     /locus_tag="OO585_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6629184.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12358..12831)
                     /gene="creA"
                     /locus_tag="OO585_001556"
     CDS             complement(12358..12831)
                     /gene="creA"
                     /locus_tag="OO585_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6629185.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13042..13911
                     /gene="robA"
                     /locus_tag="OO585_001557"
     CDS             13042..13911
                     /gene="robA"
                     /locus_tag="OO585_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6629186.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13908..14555)
                     /gene="gpmB"
                     /locus_tag="OO585_001558"
     CDS             complement(13908..14555)
                     /gene="gpmB"
                     /locus_tag="OO585_001558"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6629187.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14607..15128
                     /gene="yjjX"
                     /locus_tag="OO585_001559"
     CDS             14607..15128
                     /gene="yjjX"
                     /locus_tag="OO585_001559"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6629188.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15213..15539)
                     /gene="trpR"
                     /locus_tag="OO585_001560"
     CDS             complement(15213..15539)
                     /gene="trpR"
                     /locus_tag="OO585_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6629189.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15629..17566)
                     /gene="sltY"
                     /locus_tag="OO585_001561"
     CDS             complement(15629..17566)
                     /gene="sltY"
                     /locus_tag="OO585_001561"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6629190.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17777..19444
                     /gene="ettA"
                     /locus_tag="OO585_001562"
     CDS             17777..19444
                     /gene="ettA"
                     /locus_tag="OO585_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6629191.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19751..20983)
                     /gene="nadR"
                     /locus_tag="OO585_001563"
     CDS             complement(19751..20983)
                     /gene="nadR"
                     /locus_tag="OO585_001563"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6629192.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21004..22386)
                     /gene="radA"
                     /locus_tag="OO585_001564"
     CDS             complement(21004..22386)
                     /gene="radA"
                     /locus_tag="OO585_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6629193.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22435..23403)
                     /gene="serB"
                     /locus_tag="OO585_001565"
     CDS             complement(22435..23403)
                     /gene="serB"
                     /locus_tag="OO585_001565"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6629194.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23509..24153
                     /locus_tag="OO585_001566"
     CDS             23509..24153
                     /locus_tag="OO585_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6629195.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24181..25197
                     /gene="lplA"
                     /locus_tag="OO585_001567"
     CDS             24181..25197
                     /gene="lplA"
                     /locus_tag="OO585_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6629196.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
BASE COUNT         6071 a         6997 c         6292 g         5962 t
ORIGIN      
        1 ctattaccaa tttgaccgcc attttactgc tctcgcctgt ggttcatacc attgccagtg
       61 attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg
      121 atatcggtcg ccagctttct ccggacgcgt gggatgatgt ttcgcaggag taatcacaac
      181 tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg caaggactgg
      241 atatgctgat tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca
      301 cgcgctatac gctgccggag ctgttagaca attcccagca gttgatccat gaagcgcgga
      361 aactgacgcc tccgcagatt agcacgctga tgcgcatcag cgacaaactg gcgggtatca
      421 acgccgctcg ctttcatgac tggcagccag atttcacgcc ggcgaatgcc cgccaggcga
      481 ttctggcgtt taaaggtgat gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg
      541 atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc gtactgcgcc
      601 cgctcgattt aatgcagcct tatcgtctgg aaatggggat ccgtcttgag aatgcccgag
      661 ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac gaggcgctcg
      721 cagcacaagg cgataatgtg gtgattaatc tggcgtcaga tgagtatttt aaatcggtga
      781 agccgaagaa attgaatgcc gagattatca agccggtgtt cctcgatgag aagaacggca
      841 aatttaagat catcagcttc tacgctaaga aagcacgcgg tctgatgagt cgtttcatta
      901 ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa ggttacttct
      961 ttgatgaagc ttcctccagc aatggcgaac tggtgtttaa acgctacgag cagcgttaag
     1021 tttgcagtca atgccggatg cggcgtgaac gccttatccg gcctacaaaa gcatgcagat
     1081 tcaatatatt gcagaattca cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct
     1141 gaatgccgga ttgttacccg gcatttgtca tttagcggtg atgtttgcct ggaccatgac
     1201 cgccgtgatg atcatgagga gctttcttat ggtggcgcgg cggtggcggc ggtccgtgtg
     1261 ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg tcgcgccagt
     1321 gacctccatc ccagtaatag ccacgattat cacgattgcc tatctgtaat tttactgacg
     1381 ggactaacgt aatttccgca gcctgtgctg ccatgggagc gaccagaacc agggaaagtg
     1441 cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc ccgttatggg
     1501 tcgatatccg gagattacgc gatgagaacg acggttgtta ttctccgcaa tcctaatctc
     1561 taagttaccg catttattga gaatatctcc tttttccctg tcgtttttct ccataatttc
     1621 ttcataaaaa agccgggctg cataaaagca aacccggcct gattgagata atgaatagat
     1681 tttactgatg attcatcatc aatttacgca acgcagcaaa atcggcgggc agattatgtg
     1741 aaagcaaggg taaatcagca cgttctgcca gctcttttgg cagatccaac gtttcaccga
     1801 gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat
     1861 attcgcctgg attcaactgg tcacgcagcg cacgatacgc tacggcagcg tgcggctccg
     1921 aggtgtagcc cagttctttt aactcacgca ttgtctgttg cgtggtttca tcatccacgg
     1981 ctgcataacc cagctctttc agttgccaga ttttgcggcg gaacaactct tccacacgcg
     2041 gccagttgtt cggctgactc acgtccatcg cgttggataa cgtcgcctga gtcgctttgg
     2101 gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa
     2161 taaaacgttt caccggcaga cagagtgact tcgccagcag acccgccgtc aaatcgccga
     2221 agtttccgct tggcaccgag acaaccagct ggttgcgcgc ttcctgcggc agctgcgcaa
     2281 ctgcttcaaa gtagtagcaa atctgcgcca gcaaacggct gatgttaatc gagttagctg
     2341 agtttaaccc cagcgccact ttcagctctt catcatcaaa cgcctgcttc accagcgcct
     2401 gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat attgccgccc aatgtacaga
     2461 acagtttttc ttgcagtgga ctgattttgc ctcgtggata gaggataacc actttcacat
     2521 tcggtaaacc gtagaaagca tgagccactg ccgctccggt atcaccggag gtcgcggtca
     2581 gaatggtcac tggcttatcg cccgcaatat gggtcagcat ttgtgccata aagcgaccgc
     2641 cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg acatcgcttt
     2701 caacattggc gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct tccaggattt
     2761 cctgcgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg gtgacaaaat
     2821 ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa
     2881 acagcccctg atttttgccc aacccctggg ttacggcttg cgcaaagctg acctgctcat
     2941 tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg cccgccgtat
     3001 ccagccggca aatatgaaca aaaccttcct gattttgcag atagttctta cccaaccagt
     3061 cggcaacgcg ctgggcggta tccggcttgt cacacagagc gaacaaggtc gggccggagc
     3121 cggagatacc gctcgctacc gcgccgattt ccgcaaccgc ctgccgcgcc tgccggaagc
     3181 caggcagtaa ccgttcacgg tagggttcag cgataacatc tttcatcagc ttcgcggcaa
     3241 gctcaggctg acgggaatag caggcgtgaa tgaagccagc cagatgtcgc ccgtgcgcaa
     3301 tgcaatcctg gcggcgatac tgcgccggta aaatagccct ggcttctgcc gttgagactt
     3361 taatccccgg atacgccagc acccacagcc actcatcaaa ccctggcact tgctggctga
     3421 tgatgtcgtt ttcttcgatc atcaactgca taccaccaag aaaacacggt gccacgttgt
     3481 cgtaatgaat gctgccggag attcgtcctt ccagctcgcc catcaaagcc agcaaacgag
     3541 tgtcattaag cggcttgcca cagtgttcat tcatcgccat cagcgccgcg accaccgaac
     3601 aggcgctgga gcctaagccc gaaccgattg gcatattctt ttccagagtc atcgccactg
     3661 gaatttgctt gccaagctcc tggcaaaaac gctcccagca ctgataaacg atattttccc
     3721 gtggttctga cggcagctta tcggcaaagc gtccgaggtt gttgagactg aatgtctctg
     3781 ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacaggtgtc accgccgccc
     3841 cgagcacatc aaacccgacg ctcatattgg cactggaagc cggggcataa actttaacca
     3901 tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac accggcagct
     3961 gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg gcagcggctg ataatagtgg
     4021 ctataaaagg ccagggcgtt ttcgccattt ttcactttga acagcggatc attaccatcc
     4081 acttcggcaa tcttcacgcg gcaggcacca tcttcatcaa tattgccaac atagcgcaaa
     4141 acttttcctt catcacgggc cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac
     4201 agattcgcca taaaagcggc aacatcaccc tcagcgttaa actctgcggg cagcacaggt
     4261 tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag aatcaatagc
     4321 ttacgcgcta catccatacc agaaagatca tctcgcggat ccggttcggt ataacccatt
     4381 tcccgcgcca gagtagtcgc ctcggagaaa ctcatgcctt cgtctaactt gccgaagata
     4441 taagaaagcg aacctgaaag aatgccggag aacttcatca attcatcacc agcattgagc
     4501 agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc atagaggaat
     4561 ttacgccgcg atttttccgc cgcatgacgc aacagatggt agtaatccat cgacgaggtg
     4621 ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc gcaagaagtc ggcatattga
     4681 tccgccactg cctggctgga ggtgcagtca acaatgaccg ggttcagcag atgatattct
     4741 ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgcgc cagttcttcc
     4801 tgccagtttt ccagatttag gccatgcaca ttggtgagca gtgccttcga gttggcaaca
     4861 ccgcagacac gtaagtcgat atgtttattc ttcagccagc tttgttgacg cttcagttgc
     4921 tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat aacctgatcg
     4981 gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc gttatttacc
     5041 acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt gatattggcg
     5101 cgggccagcg cggcaaagaa tttcgccgag atcccacgca aggtgcgcat accatcacct
     5161 accaccgaga taatggccag ccgttccgtc accgccagcg gctccagtaa gccttctttc
     5221 agttccaggt agaactcttc ctgcattgcc cgttcagctc gcacacagtc gctttgtgga
     5281 acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg
     5341 gcgcgtgaca ttgcagcaaa gacgcgcgcc gccatgccga ccattccttt catccccgga
     5401 ccagaaacgc tgaacattgc catattattc agattggaaa tgcctttgac cggtaattcg
     5461 tcttcatcac ggctggcacc aatgagcgta cctggtgctt gaggatttcc ggtattttta
     5521 atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta
     5581 gcgccgaagt aggaaagctc catcgcttcc tggtaggaca tcgacttcaa caacctcgca
     5641 tcgggcacct gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa
     5701 caatcggcgc gtaaacaggc agccagcacc gccgcggagt agtcggaacc gttacgtcca
     5761 agtaccacca gttcgccttt ttcattaccg gaggtgaaac ctgccatcaa caccatgtga
     5821 tcagccggaa tgcggcttgc cgcaatacgg cgggtggact cagcaatatc gacggtagat
     5881 tcgaggtaat gccccactgc cagcagtttt tcgaccggat cgataacggt aacgttgtga
     5941 ccacgagctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc acggcaaatc
     6001 agcgcagcgt tgatgctatc cgggcactgc cccaacaaac taatgccatg caggacatgt
     6061 tttatttgga caaattcctg atcgacgaaa gttttcaatt gcgccagcgg gaaccccggc
     6121 tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac gttcggcatc gctgatattg
     6181 ggtaaagcat cctggccgct aatggttttt tcaatcatcg ccaccaggtg gttggtgatt
     6241 ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct ttccagaata
     6301 tcggcaacac gcagaaaacg ttctgcattt gccactgatg taccgccgaa cttcaacact
     6361 cgcatggttg ttacctcgtt acctttggtc gaaaaaaagc ccgcactgtc aggtgcgggc
     6421 ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt
     6481 ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa tttttatctg
     6541 tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat aaaattttaa
     6601 tttactcacg gcaggtaacc agttcagaaa ctgctatcag acactctttt tttaatccac
     6661 acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt
     6721 tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc
     6781 tcgttgctct aaaagcccca ggcgttgttg taaccagtcg accagtttta tgtcatctgc
     6841 cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca gttgatgttg
     6901 gtctgccgtt gtgtcacttt tcgtcggttg ttgtattaat gttgctaatt gatagcaata
     6961 gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg gcacaccagt
     7021 aagaacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc gaccaaacac
     7081 cagcgcggca tggctcatcc atgaagattt ttcctctaac agcagcacca gttctactgg
     7141 cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg gtggcgacag tgaaatcgac
     7201 atcgtgtaac gattcaacca gtgtcgggaa aactttaata ttatcaataa tatcaccaga
     7261 tccatgtgcg acccagcgag tggctggctc cagatgtgcc tgactatcga caatccgcag
     7321 ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt ctgctctggc
     7381 gggtgcgacc agaataatcg ttatacgcat attgccactc ttcttgacca aatgaccgcg
     7441 aacagggtga tcactgtcaa cttattacac ggtgcgaatt tacaaatcct taacgtaagt
     7501 cgcagaaaaa gccctttact tatcttaaaa aaggctaaac tatttcctga ctgtactaac
     7561 ggttgaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat tttataaaaa
     7621 ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac tagttggatt
     7681 attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa actgttaacg
     7741 tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg tccggcccct
     7801 cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg acactgtcgg
     7861 gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga tggaaagtgc
     7921 atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat ttgcagcatg
     7981 catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca atttaggtag
     8041 caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca cgcaacacgt
     8101 tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat ggcgcggaaa
     8161 tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc aatctgccgg
     8221 gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt
     8281 tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg
     8341 actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt
     8401 cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa agctacaagt
     8461 tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat ggcgagcagt
     8521 acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa
     8581 ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa ccacacgacc
     8641 gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg ccggatacgc
     8701 cggaaatcat cgccaccatc cacggtgaag gttatcgctt ctgtggtgat ctggaagatt
     8761 aatcggcttt accaccgtca aaaaaaacgg cgctttttag cgccgttttt atttttcaac
     8821 cttatttcca gatacgtaat tgatcgtcca ttgtaacttc tttactggct ttcattttcg
     8881 gcagtgaaaa cgcataccag tcgatattac gagtcacaaa catcatgccg gccagcgcca
     8941 ccaccagcac actggttccc aacaacagcg cgctatcggc agagttgagc agtccccaca
     9001 tcacaccatc cagcaacaac agcgcgaggg taaacaacat gctgttgcgc cagcctttca
     9061 ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg attatccatg
     9121 ccacggtaaa accgatatgt tcagaaagcg ccagcaagag caaataaaac atcaccaatg
     9181 aaagccccac cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa
     9241 caaagaacgc cataaaagtc agtgcaatca gcagaatggc gtacttagtc gcccagtccg
     9301 ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc
     9361 agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac cagctgcttt
     9421 gccattgcgc ctgaaaacct gactcgctaa cttcccgttt ggctggtaga aaatcaccta
     9481 aaaaactggg atgcggccag ttgctggtta agggcatttc gctattacgc ccggcaggca
     9541 ccacagaaag atcgccggta ccgcttaaat tcagtgccat attcagctgc aggttctgct
     9601 tccgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc tctaacccgg
     9661 taccgggttc aatggtcagt gccgttccgt taacttcagg cgctttcacc acaccaatac
     9721 cacgcgcatc cccgacgcta atcacaataa agggcttgcc taagatgata tttggcgcgt
     9781 tgagctcgct aagacgcgaa acatcgaaat cggcttttag cgttaaatca ctgtgccaga
     9841 cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca tcaaccatca
     9901 atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt ttatcctctt
     9961 cctgcaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaacg agtttttgcg
    10021 gcccgctggt actttggcga atcgcatctt ccacatcgct acggtaatcg gcacgttcga
    10081 caatcacttg ccgaatcagc attatcggaa tcaacaacag caatactgca ccaaacaagg
    10141 tggtcatttt ccagaacagg ggggatttca acatagtctt ctcctttgca atggcagcag
    10201 gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg
    10261 aggccagcac gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg
    10321 cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt gcacgcggca
    10381 aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca ctaccggtat
    10441 ccagcacctt aagggcgacg tgttcctgat ccacttcggc gcttagcgtt atgcgaccgc
    10501 tctcgggggt aaaatcgatg gcgttatcca gtaaattccc cagcgcctgc tccagtaacg
    10561 ccggttcagc agcaacatta acctcagtgg gcataacatg tagagtgatg tttttttctg
    10621 ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa tgccgccaca tcaacaacag
    10681 tcagaacgac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt tctaccaatg
    10741 cctgcatacg cgcattttgc gtcagaatgt tgtcggtaaa acgagccacc acttccggtg
    10801 gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg ctttttagct
    10861 catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc ttcacgcgca
    10921 tactttccag cgcctgcgca agcttacgca actcgctact gccgagttca ggaagaggaa
    10981 cgggcttatt gtcagtgacg gaatcggcat agcgagtgag ccgggcaatg gagcggttga
    11041 tccaccaaac catgcctgcg ccaatcacca gtgcaatccc cagcaaaatg gcgctggccc
    11101 ataaaattcg ccgctcgcta cgcttaatga ccggagccat cgccgcgttc ggtttgccta
    11161 cgctcaaaac gccaataagc cgcgagccgt ccataatcgg cgcggcaaca tacatcaccg
    11221 aactttcggg atcggcagga ttttgcaacg tgctgcgcgc accatactga ccacgcaacg
    11281 ttagccagac gtcattccag cgcgaataat cctgcccaac ggctttattt gccgaatcga
    11341 acaatacttt accctgcgaa tcggtcatat agacatgata ttcattgcgc actttgttaa
    11401 tgccaccgat attggcgcga aacggacgat gttgtagctg attaaacgcc tgcgccagtt
    11461 gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgcc agcaacgttg
    11521 cggtgtcgat caacgtcccc tccgttgctc ttcgcacgcc cggtttaact tctttgacaa
    11581 aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc agcagcaacc
    11641 gcatgccgat acgcattaca ggcccctcag gctatatccc atgccgcgat gagtattaat
    11701 cggtgaaaga tcggggttga tggcacgcag cttggcatgc aacgttttaa tgtgggtatc
    11761 aacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatca gttgctggcg
    11821 ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact cataccgcgt
    11881 cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca attcaaaatg
    11941 accaatacgg atgacgggag acggcgacga gaacttcttc acccgacgca gtaaggtgcg
    12001 caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat
    12061 ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa
    12121 cgccggatgg agcgccagta actggctgca caattcaaag ccgctgatat caggcaaacc
    12181 aacgtcgaga atcatgacgt cgggaaccag ctggcgagct ttatccagca ccggcaagcc
    12241 tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc
    12301 tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgtataaatc gcctctgtta
    12361 ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac
    12421 tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac
    12481 ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc cctgagcttt
    12541 gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag aaatagccgc
    12601 atcggaggta tcttccgcca gacccaatcc ccctttaata ccacctgttt tcgcccggct
    12661 cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa caattttgtg
    12721 atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg catgagccaa
    12781 tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca tattgttacc
    12841 atttttatct attacacgag caattagttc gtcacggtaa ataacatatt gattaagaac
    12901 aaaatctcaa ttcttttatt tccgtcaagc cctacaacat actctactaa gaaaaaaaca
    12961 ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag gtgctcgttg
    13021 ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct tttaatctgg
    13081 ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat
    13141 tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat tggcgcgtat
    13201 attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt
    13261 ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg cgcattcaag
    13321 aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag cgcctttggt
    13381 attcgcccgc cgctacgtct gggtgaattc acaatgccag agcacaaatt tgtcaccctg
    13441 gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga gcaaatctct
    13501 gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc
    13561 attccgccag tgctctacgg cctgaatgaa acgcgtccga gtcaggataa agacgacgaa
    13621 caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta tgtactgacg
    13681 gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga aggtctggga
    13741 accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat gctcaacctg
    13801 acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc caaagcggga
    13861 gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta acgctgcagc
    13921 tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac gacccagcca
    13981 gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt
    14041 aagcggcgcg ctgcccatgc tggtaatccg agaatcgtac tcaccagaca tcccagtgca
    14101 ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca agattccagt
    14161 gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat acgcccgtca
    14221 acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag agaatcgata
    14281 tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa gatgatgtca
    14341 cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa atcgctactg
    14401 ataatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc ctgttgctca
    14461 cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc gttccactgc
    14521 gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat actgctgtaa
    14581 cgttaatttt ctgagtaatg ctgattatgc accaagttgt ctgtgcgacc accaatcccg
    14641 ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga tcctgccata
    14701 ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc ctttggcagt gaggaaacgc
    14761 gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag gctgattttt
    14821 gggtggcgat tgaagctggc atcgatggtg acagcacttt cagttgggtg gtgattgaaa
    14881 acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc gttacctgcg gtgattctgc
    14941 agaaagtgcg tgaaggtgag gcgcttggtc ccgtgatgtc gcgttatacc ggtattgatg
    15001 agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc actcgcgcca
    15061 gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc gtgtatcagg
    15121 cattgtagga cggataaggc gttcacgccg catccggcat tcggtgcacg atgcctgatg
    15181 cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa cacctcttcc
    15241 agccactggc gcagctccac gggcgcggct ttcaggctgt tagatccacg cgtaatcgtc
    15301 gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc acgcaacagc
    15361 tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt cagcatcagg
    15421 tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc gacaaaacgt
    15481 aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg ttgggccata
    15541 atatgtcgcc attgttagcg ggggaagcaa aatgcctccc ccggttgtac tcgctaaaga
    15601 gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc gtggcgctca
    15661 tcaacgtcgg tttatccccc atgaaataac ggtagtaagc gtcataagcc agcacgttct
    15721 tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca gcgtcgatac
    15781 gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta taagctgctg
    15841 aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa ctggtgccaa
    15901 tgttgatatt cgtttccgga tccagcaatt gcccagggct gctataacca ggaatagaga
    15961 acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg ctggccccta
    16021 ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc atcgcatagc
    16081 tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa gccagcggga
    16141 atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg ctaagatccc
    16201 accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt gacttgctct
    16261 tcaccagatt ggcccactcg ctacgcgcgg tgttatcgag attccagtac atcaactcgc
    16321 gcacgcgcgc catctccggc ccctgagtca gggcgctgtc aacattctgc ggcgctttat
    16381 caatcttcag ctcatactct tctccgatgc gttgtgctgc aaccatcggg tagaaaccac
    16441 gctgttgcat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg cgttccagca
    16501 ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc ggcagacgcg
    16561 ccagccaggt attcaggccg cgacgatcgc cggtgccaag tgccattcgc acacggcgtt
    16621 caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc gcctgctcgt
    16681 cggtgacatc gttgcccatc aaacgccagg cgacgatatc gcgcagctcc tgaatctgat
    16741 cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca ttttcagcat
    16801 cctgccgcgc cacactggca aacgccaccg ctgccatttg acgggtaaaa tcggtcgctc
    16861 cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa atgattgccg
    16921 aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc aggccagtgt
    16981 tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct tgtttacctg
    17041 acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg ccggttagcc
    17101 acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat ttcgcatagt
    17161 agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct aacaagccac
    17221 gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg cgagcgggag
    17281 gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga ttcatcagat
    17341 catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc agtccaggca
    17401 tcatttgttc gaccacatcc atttgtcgat tatcccaggc ctgcttgatt tgcgcgtaac
    17461 ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc agcagacaga
    17521 caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc gcatcctcta
    17581 agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa ccagtcattg
    17641 ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat cgaccaactg
    17701 ggattaggcg gggaaaaagg ctacacttcg cctttgaaaa ctcatcatca cgataaacat
    17761 agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg gcaaagttgt
    17821 tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg gggcaaaaat
    17881 tggtgtcctg ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta tggcaggcat
    17941 tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg gttacctgcc
    18001 gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag aagcggtttc
    18061 tgaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg ccgatccgga
    18121 tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca ttcaggctca
    18181 cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctac gtctgccgga
    18241 ctgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtag cgttgtgccg
    18301 cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc acctggatgc
    18361 cgaatccgtg gcctggctgg aacgcttcct gcacgacttc gagggcaccg tggtggcgat
    18421 tacccacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac ttgaccgcgg
    18481 tgaaggtatt ccgtgggaag gcaactactc ctcctggctg gagcagaaag atcagcgcct
    18541 ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag agctggagtg
    18601 ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg cacgctttga
    18661 agagctgaac agcaccgaat atcagaaacg taacgaaacc aacgaactgt ttattccacc
    18721 tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat cctacggtga
    18781 tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcaatcg tcgggatcat
    18841 cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctctggtc aggaacagcc
    18901 ggacagcggc accatcactt taggtgaaac ggtgaaactg gcatcggttg atcagttccg
    18961 tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc tggatattat
    19021 gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta actttaaagg
    19081 ggttgatcag ggtaaacgcg ttggtgaact ttccggtggt gagcgcggtc gtctgcatct
    19141 ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa ccaacgacct
    19201 ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg gctgtgcgat
    19261 ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc tggactacca
    19321 ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag agtacaagaa
    19381 acgcacgctg ggcgcagacg cactggagcc gaagcgtatc aagtacaagc gtattgcgaa
    19441 gtaatgcgta aaatgccgga tgcggcgtga acgccttatc cggcctacaa accaagaaat
    19501 ttcaatacgt tgtgaatctc caggcctgat aagacgcggt aagcgtcgca tcaggcattg
    19561 agcaccgaat gccggatacg gcgtgaacgc cttttccggc ctacaatagc cttagtatcc
    19621 gcaatttcgt agcgggtcac ccgtgctgat tcttttaaac cttgtttaat ctcgacagcc
    19681 tcttccagag aggttttcaa ctcatcaaaa aacttatttt atacctcgcc tttgagccgt
    19741 ttcatcgcgg ttatctctgc tcccccatca tctcccgcac cagctccacg cagcgcagga
    19801 aacggctgtc ataatcgtcc tcttcaaccc gcacgaattc gatattgttc tcttccagca
    19861 tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg ctgcgtaaac
    19921 catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa cggtattcat
    19981 caatcaacgc ctgtacgaac ggatgctcac gcccttcgta ctttttgcag aacgcctggg
    20041 tagtgacaaa atcagtgtca ataaacgcca ctttattggc atatttcact gcaaaatcaa
    20101 tgtattgtgc gtggcccagc gcgattttat cgtaatcgga atactgcaat gcgatctcat
    20161 caccgccgag gtgtgaaaag acataatcgc gaccatattc ccacgcactg gtggtattga
    20221 agatattggc aagtttgttt accagggtgg atttaccgct cgactcgcca ccaaggatcg
    20281 ccacggtacg tacaaagaac ggcttcactt ccgtaggaat atattcccag tagcggaacg
    20341 ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga tcgaccagca
    20401 ccgtatcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc gaggtgtaga
    20461 tcaggtccgg ctgaatcccc ttttcagcca taaacttttt gatgccgttg ctccacacat
    20521 cccagccgtg cggatacggc tccatgccct cctcgttgaa agcatgaatg cgaatatttt
    20581 tctgatattt aaaagtttgt aataaccagc gcagacgatc cggcaccgtg ggctgctgcg
    20641 acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat
    20701 gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg gtatgcagcg
    20761 ggtagaactt accgaatacg acaccaatgg ttttcttctg tcggggaaat tcaagcccca
    20821 aaaaacggtg caacgcctcc agcttttgcg cgctggggct tttaattttg gcattcagta
    20881 actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt agcgtgcagc
    20941 cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc tccgtatctc
    21001 tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc caaaaatctg
    21061 catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc
    21121 gtgtttcgcc gcttctgaaa ttcgttcctg accgctgggc accgggcgga tctcccctgc
    21181 cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga
    21241 aaccatcgcc agcagtaacg ctaaatcggc actggtttcg gttaccttca cgccgccgac
    21301 cacgttcaca aatacatcct gatcggccat ttgcagacca ccgtgacggt gcaagacagc
    21361 cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg gattcgccat
    21421 catcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc cttcccacac
    21481 caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa ttgccgaagg
    21541 gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct cattcaccgc
    21601 gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat caccatccag
    21661 aagcaccgaa cagtcgatac agtgttccag cactttaggg ccagccagcg agccatcttt
    21721 ggttacgtgc ccaaccatga caatcgccac accgcgcgtt ttggcgaagc gcgtcagata
    21781 agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat ccgccatatg
    21841 catcacctgg atcgagtcaa ttaccatcag tttcggttgc tcttcttcgg caatcaggca
    21901 gatctgttcg atgctggttt ccgacaacat attgagattg tcagtcggca ggccaaggcg
    21961 atgagcgcgc attgccacct gttgcagcga ctcttcgcca gtgacataca gcgttttcat
    22021 ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg gatttccccg cacctgggtt
    22081 accgccaatc agaatggcgc ttcccggcac cacgccgccg cctagtacgc ggtcgaactc
    22141 tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga gtttctggac
    22201 ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg
    22261 cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac tgcactgccc
    22321 ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt ttggagcttt
    22381 tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tggcgggcga gcaattactt
    22441 ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt gacggatggt
    22501 gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca gccctgctgc
    22561 tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg ccagcgggat
    22621 ttcatactcc tgcgcgaggc gagtcaaagt tttcgctttg tactgcgcgt ctacgatgtc
    22681 gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg ctaccacggc
    22741 ggtcaggcgc agcttgtcgc gcagatattc agcaaagaaa gtaaagccgc cggaggcaat
    22801 cgccactttc cagcccagcg tttccagctt gagcaccagt tgcgttaagc ctggcatcag
    22861 cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca gcgtcgccac
    22921 acggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt cggttacttc
    22981 cgccaccatc tcgcccgttc cggccagttt ggcaatttca tcaatacatt caatctggat
    23041 ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc
    23101 cagcggggcg acatccagtt gcgcttcatg ggccaggcgc gtagcccgtg cggtgagtga
    23161 acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa tcaccatcgc
    23221 cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac gaccatacag
    23281 cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac cacttaatga
    23341 aagaggcaga ccaggccata aagagacatc ttcaggcagg tcgcaccagg taatgttagg
    23401 cattaaggat cctgtaaaat cgttcgaagc agggaaaata acgcatgagg ctaccttgta
    23461 tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat ggctcgcaca
    23521 aaactgaaat tccggctgca tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg
    23581 gcgctgatgc agggagcgtc atggtttagt caaaaccacc agcgacaacg taatccacag
    23641 ctggaagaac tggcccgcac cctggcgcgt caggtaacgc tgaacgttgc accgctgatg
    23701 cgtaccgact caccggatga aaaacgcatt caggcgatcc tcgatcagtt aacggatgaa
    23761 agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc acgttctggc
    23821 gaaagcgtcg aagtgcgcga ccggctggcg ctcgacggta aaaaagcggg cggctatttt
    23881 aaccagcaga ttgtcgaacc aattgccggg aaaaacggac cgctcggcta tctgcgcctg
    23941 acgctcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac cactaacatt
    24001 ttacgcctga tgttgctgct ttcactggca atcggcgtag tgctgacccg cacgctgcta
    24061 cagggtaaac gtacccgctg gcagcaatcg cccttcctgt taaccgccag caaaccggtg
    24121 ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt
    24181 atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa cctggcggtg
    24241 gaggagtgta tttttcgcca gatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat
    24301 gccgacacgg tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg
    24361 atggaagaag ataacgtccg cctggcacgg cgcagtagcg gtggcggcgc ggtgttccac
    24421 gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga taaaaccatc
    24481 tccacgtcga ttgtgctcaa tgcgctgaac gcgcttggcg tcagcgccga agcgtccggg
    24541 cgtaacgatc tggtggtgaa aaccgccgaa ggcgaccgca aagtctcagg atcggcctat
    24601 cgcgaaacca aagatcgtgg cttccaccac ggcaccttgc tgctcaatgc cgaccttagc
    24661 cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg cattacctca
    24721 gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca tgagcaggtt
    24781 tgcgaggcca taaccaaggc ctttttcgcc cattatggcg agcgtgtaga agcggaaatc
    24841 atctccccgg acaaaacgcc agacttgcca aacttcgccg aaatctttgc ccgtcagagt
    24901 agctgggaat ggaacttcgg tcaggctccg gcattctcgc atctgctgga tgaacgcttt
    24961 agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac ccgcgcccag
    25021 gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg actgcaaggc
    25081 tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt tgacttcccg
    25141 gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt aaggtaactc
    25201 gctgattaaa ataccaaagg aagggaatat gatcgatctg gaaaaccagg agcgggagat
    25261 tatcaattta atgttaagcc agcgtatttc ctggctagcc gccgtgcgta tcaggcataa
    25321 at
//
LOCUS       DAJTYD010000042        25012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTYD010000042 DAJTYD010000000
VERSION     DAJTYD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.042"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..808
                     /locus_tag="OO585_001568"
     CDS             <1..808
                     /locus_tag="OO585_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR6629197.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO585_001569"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO585_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6629198.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSVAILLLVITLWLCNSKRLKRE"
     gene            1520..1798
                     /locus_tag="OO585_001570"
     CDS             1520..1798
                     /locus_tag="OO585_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629199.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="OO585_001571"
     CDS             complement(1845..3941)
                     /locus_tag="OO585_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6629200.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIGGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="OO585_001572"
                     /pseudo
     CDS             complement(3941..4678)
                     /locus_tag="OO585_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            complement(4675..5313)
                     /locus_tag="OO585_001573"
     CDS             complement(4675..5313)
                     /locus_tag="OO585_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6629201.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /locus_tag="OO585_001574"
     CDS             complement(5427..5669)
                     /locus_tag="OO585_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629202.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO585_001575"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO585_001575"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6629203.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="OO585_001576"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="OO585_001576"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6629204.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9906..10178
                     /locus_tag="OO585_001577"
     CDS             9906..10178
                     /locus_tag="OO585_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6629205.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO585_001578"
     CDS             complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO585_001578"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6629206.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11531..12478
                     /locus_tag="OO585_001579"
                     /pseudo
     CDS             11531..12478
                     /locus_tag="OO585_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            12548..13354
                     /locus_tag="OO585_001580"
     CDS             12548..13354
                     /locus_tag="OO585_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6629207.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTHG"
     gene            13364..13771
                     /locus_tag="OO585_001581"
     CDS             13364..13771
                     /locus_tag="OO585_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6629208.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13771..14265
                     /locus_tag="OO585_001582"
     CDS             13771..14265
                     /locus_tag="OO585_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6629209.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14331..15128
                     /locus_tag="OO585_001583"
     CDS             14331..15128
                     /locus_tag="OO585_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6629210.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15139..15981
                     /locus_tag="OO585_001584"
     CDS             15139..15981
                     /locus_tag="OO585_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6629211.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16015..17244
                     /gene="sorE"
                     /locus_tag="OO585_001585"
     CDS             16015..17244
                     /gene="sorE"
                     /locus_tag="OO585_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6629212.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17627..18316
                     /gene="pepE"
                     /locus_tag="OO585_001586"
     CDS             17627..18316
                     /gene="pepE"
                     /locus_tag="OO585_001586"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6629213.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEDNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18407..20038)
                     /locus_tag="OO585_001587"
     CDS             complement(18407..20038)
                     /locus_tag="OO585_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6629214.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20258..23946)
                     /gene="metH"
                     /locus_tag="OO585_001588"
                     /pseudo
     CDS             complement(20258..23946)
                     /gene="metH"
                     /locus_tag="OO585_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            24146..24970
                     /gene="iclR"
                     /locus_tag="OO585_001589"
     CDS             24146..24970
                     /gene="iclR"
                     /locus_tag="OO585_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6629215.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5883 a         6617 c         6532 g         5980 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgc
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctctgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcaaccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccgaca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgctccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgccga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaggccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagttgttt gccagccgcg gttagcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac caaggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aagtcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa acttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttctttg tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tttgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
     9961 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
    10021 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
    10081 aaaaagcccg ccaattgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta
    10141 gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt
    10201 ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga
    10261 ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc
    10321 ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt
    10381 tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc
    10441 ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac
    10501 tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata
    10561 atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc
    10621 tgacggttca gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact
    10681 ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat
    10741 tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca
    10801 cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct
    10861 ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta
    10921 tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt
    10981 accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg
    11041 ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg
    11101 atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa
    11161 cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct
    11221 tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt
    11281 ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat aaggataatc
    11341 aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga aacaaatgtg
    11401 caagagaaag cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca
    11461 tgatggggtt atatcagatc aagcggtgag aaagcatatt tgcacaaatg tgcagaacca
    11521 ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta
    11581 ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac
    11641 catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta
    11701 tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga
    11761 cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt
    11821 acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg
    11881 gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt
    11941 aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa
    12001 cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc
    12061 ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac
    12121 catctctgcc tactgagata atctggatgt cgccctggtg ggaattggct caccggccat
    12181 tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc
    12241 ccgccaggtt gctgacgata tttgctcgcg cttttttgat attcacggcg caatggttga
    12301 aacgaatatg agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata
    12361 ttccattggc attgccatga gtgaagaaaa atacagcgga attgttggtg cactgcgtgg
    12421 aaaatatatt aattgtctga taacgaatag cagcacagct gaactattac tgaaataaaa
    12481 atcttcccga tgtttagcgt aataaaaatt acggcggggc cctattatct aaaatcagga
    12541 gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca ccggcggcgc
    12601 atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat
    12661 ggtagatatt cacggtggcg atggtcaata tgaaggtcat aaaggctatc agttttggcc
    12721 gaccgatatt tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt
    12781 tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga
    12841 tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa
    12901 tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca
    12961 acatgatggc gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca
    13021 aagctgttac gccgctacca aagccgcgct taatagcttc acgcgatcct ggtcgaaaga
    13081 attgggtaaa cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaacagg
    13141 actgcgcacg ccggaatatg aagaagcgct ggtgtggacg cgcaatatca ccgtcgagca
    13201 gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga
    13261 agtcgctgat tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac
    13321 cactaacatt gcgggcggca aaacgcacgg ctaaggaggc tttatggtca atgccatttt
    13381 ttgcgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg tgtacggcga
    13441 cgccagggtc gaggcagttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa
    13501 gctggaaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca
    13561 gtgcggcagc ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt
    13621 aatcagcggc ctttctctgc cgctggcgct cgaactggtt gataaccagg acagcatgaa
    13681 tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa
    13741 acaactggca acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac
    13801 cgcctgatcc acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt
    13861 attatctgca atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct
    13921 gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcac
    13981 aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta
    14041 gcgatggttc gccaaggggt aaaaatagac accctaaata ttggcggcat ggcctggcgg
    14101 cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt
    14161 catgagttaa ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca
    14221 atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcttgtg
    14281 tacatatgct ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa
    14341 gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc
    14401 tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg
    14461 gtgatttaaa aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga
    14521 tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc
    14581 tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg
    14641 cggcaggcca ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg
    14701 cggataaagc agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgt
    14761 ttggcgtgca ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg
    14821 ccgatatggt gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa
    14881 ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga
    14941 aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc
    15001 tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac
    15061 agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt gaccaacttg
    15121 acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg
    15181 tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt
    15241 ttttgctacg acatgatccc cgccatcaaa cgactttacc cattaaaaga ggatcaggtt
    15301 gcggcgctca ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc
    15361 atcggcgtca ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt
    15421 accattaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg cgatccactg
    15481 gtctggggaa cgctgcgccc gattaccacc gcgctcggcg catctctggc actttcgggc
    15541 aacattctcg gcccgctgct gttcttcttt attttcaatg cggtgcgtct ggcgatgaag
    15601 tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg
    15661 aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg
    15721 ctggtcacca aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc
    15781 gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg
    15841 ctggcgctcg gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta
    15901 tggctgattt tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc
    15961 agattcttcg ccccggcatg actgccgggg ccatcgctca acatgaggtg gtttatgaaa
    16021 acaacagctc tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct
    16081 gaaatgcagg aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc
    16141 tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac
    16201 cccatcatca tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag
    16261 cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc
    16321 ccggactgcc ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtggttatt
    16381 cccaacgagg tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa
    16441 ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt
    16501 caggaaggta gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc
    16561 ggcggcaccg gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac
    16621 ccgtcgcagc tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac
    16681 tatccatcag aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat
    16741 acgctaatgg cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat
    16801 gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc
    16861 gccggaccgc aggataaaca tttcagcgcg ccaattaatt tctacgatgt gcattatgca
    16921 tttacccact acgtgggcac gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa
    16981 ctgattgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat
    17041 gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat
    17101 accgggaaat acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg
    17161 attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat
    17221 gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg
    17281 ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag
    17341 aaggataaaa aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
    17401 tttatgccgg atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata
    17461 ttgcagttat ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
    17521 agtctcagct cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
    17581 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
    17641 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
    17701 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
    17761 ctgggatgat tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
    17821 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt
    17881 cggcggcggg aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc
    17941 aattactgac gtggttaaac gtggtgcgct gtatattggc tggagcgcag gcgctaacct
    18001 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
    18061 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
    18121 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
    18181 gacgattatt ggtctaccgg aagataactg gatcacagtg agtaaaggtc acgctacgct
    18241 gggtggcccg aacaccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc
    18301 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
    18361 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
    18421 gtccttcatc ttcatccgac tgttccagca cactgtaagc caccgaacaa aacagcgagt
    18481 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
    18541 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
    18601 agcgatgctt actacggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
    18661 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
    18721 catcaagcga aaatgcccgc cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc
    18781 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc
    18841 ggcgcgactc ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg
    18901 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
    18961 caccgtgcat cactttattc aacccttcca tcatctgttc catggcgtcg ccaatgcgca
    19021 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca
    19081 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
    19141 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
    19201 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
    19261 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
    19321 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
    19381 aaccgaggtt agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg
    19441 cggtcagagc cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
    19501 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
    19561 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
    19621 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
    19681 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
    19741 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
    19801 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
    19861 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
    19921 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
    19981 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
    20041 gttcaggttc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
    20101 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
    20161 ccagcctgat tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
    20221 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
    20281 tcggtgccag ccagcgctca acatctgaga cgctcatacc tttacggcgg gcatagtctt
    20341 caacctgatc gcgctgaatt tgcgccacgg cgtagtactt gctgtccggg tggctgaaat
    20401 accaacccga aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg
    20461 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
    20521 ccgggtagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
    20581 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tggagatact
    20641 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    20701 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    20761 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt
    20821 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgaca ttgatcacat
    20881 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    20941 ggcccaccac gccacgccac gcggattcag cgttttctcg gcgcttaatt tatccagcat
    21001 gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat cttccagaat
    21061 gcgcggatac ttcccggcca gcgaccaggt cataaagaac ggtgtccagt cgatgcaatt
    21121 acgcagagtt tcgatgctgg cttcaacttc ctgcacgccg agacggtgcg ccaccggcgg
    21181 cgtgtaagcc tgccagtcaa aagcgaagtc gttatcgcgc gccgcttcca gcgtgaccgg
    21241 tggtgtgcgc ggtttcttgc gcccgtgctg aatacgtacg gtttcgtact ccttgcgggt
    21301 acgagcgaca aaatcatcgc gctgagtatc ggaaagcagc gccgccacca caccaacggt
    21361 gcgcgaggca ttctgcacat acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc
    21421 cgtgtgcgct tttgaggtgg tcgcgccacc gatcagtaac ggaatggtga agccctgacg
    21481 ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgataa gccccgaaag
    21541 gccaatcaaa tcagcattca cttctttagc agtacggaga attttttccg caggcaccat
    21601 cacgccgaga tcgacaattt cgtagttgtt acattgcagc accacgccaa cgatattttt
    21661 accgatatcg tggacgtcgc ccttcacggt ggcgatcacc atcttgccgt tggttttgcc
    21721 ctgctctttg ctggcttcaa taaacggttc gagatacgcc accgcctgtt tcatgacgcg
    21781 cgccgatttg accacctgtg gcaggaacat tttgccttcg ccaaacagat cgccgaccac
    21841 gttcatgccg tccatcaacg ggccttcaat cacttcaatc gggcgcgtag cctgctggcg
    21901 ggcttcttcg gtatcctgct cgataaactc ggtaatgcct ttgaccagcg agtattccag
    21961 acgtttattc acttcccacg agcgccactc cgcctgctgg gcgttatcgg tgtcgtcggt
    22021 tttgctgccg cgatatttct cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg
    22081 attaagaatc acatcttcca ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc
    22141 cagttgcccc gcgttgacga tccccatatc catgccattg cgaatagcgt agtagaggaa
    22201 cactgcgtga atggcttcgc gcaccggatc gttgccacgg aatgagaaag aaacgttaga
    22261 tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat
    22321 aaagtcctgc gcgtagttgt tgtgctcttc aataccggtg gcgaccgcga agatgttcgg
    22381 gtcgaagatg atatcttctg gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg
    22441 acggcaaatc tcgattttcc gtgcgcgagt atcggcctgt ccctgttcat caaaggccat
    22501 caccaccact gccgcaccgt agcgacgcaa caatttcgcg tgatggataa aggcgtcgac
    22561 gccctctttc atcgagattg agttaacaat gcctttgccc tgaatacact tcagcccttt
    22621 ttcgataacg tcccattttg aggagtcgat cataatcggc acgcgagcga tatccggttc
    22681 accggcaatc agattgagaa aacgcaccat cgccgcttcg gcatcgagca tcccttcatc
    22741 catgttgata tcgataatct gcgcgccgtt ttccacctgc tgacgcgcca catcctgcgc
    22801 ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcagagccgg tgacgttggt
    22861 gcgttcaccc acgttcacaa acaggctatc gtcgccaatg ttcagcggct ccaggccaga
    22921 caaacggcag gctacgggaa tttccggcag tttgcgcggc gctaatcctt ctactgcacg
    22981 actcatcgct gcaatatgtt gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa
    23041 gcccgcttgc gcccattcac gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc
    23101 accaaaggcg ttaggcagcc cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg
    23161 tgatagttcc tgcacgtact ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa
    23221 ggttagcgct tcggcgtggc gcaatgagtt gtaaaatgct tcggtggtct gcccggagag
    23281 cgtgcgcccg gaggcgtcgg tgatggtgcc ggagatcata atcggcagct caacgcccag
    23341 cgcttcaaac tccgttttca ccgcaaaaac cgccgctttg gcgttaaggg tgtcgaaaac
    23401 ggtttcaatc aggatcagat ccgcaccacc ttccaccagc gctttggtgg attcgcgata
    23461 ggccgccacc agctggtcaa aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga
    23521 aatagacgcc gtgcggttgg ttgggccgag aacaccggca acgtagcgcg gtttctccgg
    23581 cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt ttcgccgccg caaagttgat
    23641 ttccgccgac agggattcca tctggtaatc cgccatcgca atggtcgtgg agttgaaggt
    23701 gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac
    23761 ttccggtttg ctgagcacca gcaggtcgtt gttgcctttg agatcgcatg gccagtcggc
    23821 aaagcgttca ccacgaaaat cggcttcgtt cagtcgataa ctctggatca tggtgcccat
    23881 accgccgtcc agcaccagaa tacgttcatt taactgcgca cgcagttgtt ccactttgct
    23941 gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac aaaccatact
    24001 ggcataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg ttcaacatta
    24061 actcatcgga tcagttcagc aactattgca ttagctaaca ataaaaatga aaatgattcc
    24121 cacgatacag aaaaaggaga ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa
    24181 acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc gtggcctgaa
    24241 attactggag tggattgccg aatccaatgg cagtgtggca ctcacggaac tggcgcaaca
    24301 agccgggtta cccaattcca cgacccaccg cctgctaacc acaatgcaac agcagggttt
    24361 cgtgcgtcag gttggcgaac tgggacactg ggcaatcggc gcacatgcct ttatggtcgg
    24421 cagcagcttt ctccagagtc gtaatttgtt agcaattgtt caccctatcc tgcgcaatct
    24481 aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc
    24541 gattattatc gaccaggtac agtgtacgca tctgatgcga atgtccgcgc ctatcggcgg
    24601 taaattgccg atgcacgctt ccggtgcagg taaagccttt ttagcccaac tgagcgaaga
    24661 acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg caacgctggt
    24721 gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggtt attcatttga
    24781 cgatgaggaa catgcgctgg ggctacgttg ccttgcagcg tgtattttcg atgagcaccg
    24841 tgaaccgttt gccgcaattt ctatttccgg accgatttca cgtattaccg atgaccgcgt
    24901 gaccgagttt ggcgcaatgg tgattaaagc ggcgaaggaa gtgacactgg cgtacggtgg
    24961 aatgcgctga ctttttctgg cgggcagagg caatattctg cccatcatac ct
//
LOCUS       DAJTYD010000043        24919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTYD010000043 DAJTYD010000000
VERSION     DAJTYD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.043"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..80)
                     /locus_tag="OO585_001590"
     CDS             complement(<1..80)
                     /locus_tag="OO585_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6629216.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHG"
     gene            459..770
                     /gene="rpsJ"
                     /locus_tag="OO585_001591"
     CDS             459..770
                     /gene="rpsJ"
                     /locus_tag="OO585_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6629217.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            803..1432
                     /gene="rplC"
                     /locus_tag="OO585_001592"
     CDS             803..1432
                     /gene="rplC"
                     /locus_tag="OO585_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6629218.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1443..2048
                     /gene="rplD"
                     /locus_tag="OO585_001593"
     CDS             1443..2048
                     /gene="rplD"
                     /locus_tag="OO585_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6629219.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2045..2347
                     /gene="rplW"
                     /locus_tag="OO585_001594"
     CDS             2045..2347
                     /gene="rplW"
                     /locus_tag="OO585_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6629220.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2365..3186
                     /gene="rplB"
                     /locus_tag="OO585_001595"
     CDS             2365..3186
                     /gene="rplB"
                     /locus_tag="OO585_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6629221.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3203..3481
                     /gene="rpsS"
                     /locus_tag="OO585_001596"
     CDS             3203..3481
                     /gene="rpsS"
                     /locus_tag="OO585_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6629222.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3496..3828
                     /gene="rplV"
                     /locus_tag="OO585_001597"
     CDS             3496..3828
                     /gene="rplV"
                     /locus_tag="OO585_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6629223.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3846..4547
                     /gene="rpsC"
                     /locus_tag="OO585_001598"
     CDS             3846..4547
                     /gene="rpsC"
                     /locus_tag="OO585_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6629224.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4560..4970
                     /gene="rplP"
                     /locus_tag="OO585_001599"
     CDS             4560..4970
                     /gene="rplP"
                     /locus_tag="OO585_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6629225.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            4970..5161
                     /gene="rpmC"
                     /locus_tag="OO585_001600"
     CDS             4970..5161
                     /gene="rpmC"
                     /locus_tag="OO585_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6629226.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5161..5415
                     /gene="rpsQ"
                     /locus_tag="OO585_001601"
     CDS             5161..5415
                     /gene="rpsQ"
                     /locus_tag="OO585_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6629227.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5580..5951
                     /gene="rplN"
                     /locus_tag="OO585_001602"
     CDS             5580..5951
                     /gene="rplN"
                     /locus_tag="OO585_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6629228.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            5962..6276
                     /gene="rplX"
                     /locus_tag="OO585_001603"
     CDS             5962..6276
                     /gene="rplX"
                     /locus_tag="OO585_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6629229.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6291..6830
                     /gene="rplE"
                     /locus_tag="OO585_001604"
     CDS             6291..6830
                     /gene="rplE"
                     /locus_tag="OO585_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6629230.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6845..7150
                     /gene="rpsN"
                     /locus_tag="OO585_001605"
     CDS             6845..7150
                     /gene="rpsN"
                     /locus_tag="OO585_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6629231.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7184..7576
                     /gene="rpsH"
                     /locus_tag="OO585_001606"
     CDS             7184..7576
                     /gene="rpsH"
                     /locus_tag="OO585_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6629232.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7589..8122
                     /gene="rplF"
                     /locus_tag="OO585_001607"
     CDS             7589..8122
                     /gene="rplF"
                     /locus_tag="OO585_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6629233.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8132..8485
                     /gene="rplR"
                     /locus_tag="OO585_001608"
     CDS             8132..8485
                     /gene="rplR"
                     /locus_tag="OO585_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6629234.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8500..9003
                     /gene="rpsE"
                     /locus_tag="OO585_001609"
     CDS             8500..9003
                     /gene="rpsE"
                     /locus_tag="OO585_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6629235.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9007..9186
                     /gene="rpmD"
                     /locus_tag="OO585_001610"
     CDS             9007..9186
                     /gene="rpmD"
                     /locus_tag="OO585_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6629236.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9190..9624
                     /gene="rplO"
                     /locus_tag="OO585_001611"
     CDS             9190..9624
                     /gene="rplO"
                     /locus_tag="OO585_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6629237.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9632..10963
                     /gene="secY"
                     /locus_tag="OO585_001612"
     CDS             9632..10963
                     /gene="secY"
                     /locus_tag="OO585_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6629238.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            10995..11111
                     /gene="rpmJ"
                     /locus_tag="OO585_001613"
     CDS             10995..11111
                     /gene="rpmJ"
                     /locus_tag="OO585_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6629239.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11258..11614
                     /gene="rpsM"
                     /locus_tag="OO585_001614"
     CDS             11258..11614
                     /gene="rpsM"
                     /locus_tag="OO585_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6629240.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11631..12020
                     /gene="rpsK"
                     /locus_tag="OO585_001615"
     CDS             11631..12020
                     /gene="rpsK"
                     /locus_tag="OO585_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6629241.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12054..12674
                     /gene="rpsD"
                     /locus_tag="OO585_001616"
     CDS             12054..12674
                     /gene="rpsD"
                     /locus_tag="OO585_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6629242.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12700..13689
                     /gene="rpoA"
                     /locus_tag="OO585_001617"
     CDS             12700..13689
                     /gene="rpoA"
                     /locus_tag="OO585_001617"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6629243.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13730..14113
                     /gene="rplQ"
                     /locus_tag="OO585_001618"
     CDS             13730..14113
                     /gene="rplQ"
                     /locus_tag="OO585_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6629244.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14220..14588
                     /locus_tag="OO585_001619"
     CDS             14220..14588
                     /locus_tag="OO585_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6629245.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14599..15024
                     /gene="zntR"
                     /locus_tag="OO585_001620"
     CDS             14599..15024
                     /gene="zntR"
                     /locus_tag="OO585_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6629246.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15080..15298
                     /gene="arfA"
                     /locus_tag="OO585_001621"
     CDS             15080..15298
                     /gene="arfA"
                     /locus_tag="OO585_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6629247.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15295..15705)
                     /gene="mscL"
                     /locus_tag="OO585_001622"
     CDS             complement(15295..15705)
                     /gene="mscL"
                     /locus_tag="OO585_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6629248.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15835..17211)
                     /gene="trkA"
                     /locus_tag="OO585_001623"
     CDS             complement(15835..17211)
                     /gene="trkA"
                     /locus_tag="OO585_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6629249.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17233..18522)
                     /gene="rsmB"
                     /locus_tag="OO585_001624"
     CDS             complement(17233..18522)
                     /gene="rsmB"
                     /locus_tag="OO585_001624"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6629250.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRHH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18568..19515)
                     /gene="fmt"
                     /locus_tag="OO585_001625"
     CDS             complement(18568..19515)
                     /gene="fmt"
                     /locus_tag="OO585_001625"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6629251.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19530..20039)
                     /gene="def"
                     /locus_tag="OO585_001626"
     CDS             complement(19530..20039)
                     /gene="def"
                     /locus_tag="OO585_001626"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6629252.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20169..21293
                     /gene="dprA"
                     /locus_tag="OO585_001627"
     CDS             20169..21293
                     /gene="dprA"
                     /locus_tag="OO585_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6629253.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21265..21738
                     /locus_tag="OO585_001628"
     CDS             21265..21738
                     /locus_tag="OO585_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6629254.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21767..22309
                     /locus_tag="OO585_001629"
     CDS             21767..22309
                     /locus_tag="OO585_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6629255.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22314..22886
                     /gene="tsaC"
                     /locus_tag="OO585_001630"
     CDS             22314..22886
                     /gene="tsaC"
                     /locus_tag="OO585_001630"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6629256.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            22891..23709
                     /gene="aroE"
                     /locus_tag="OO585_001631"
     CDS             22891..23709
                     /gene="aroE"
                     /locus_tag="OO585_001631"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6629257.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            23706..23963
                     /locus_tag="OO585_001632"
     CDS             23706..23963
                     /locus_tag="OO585_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6629258.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23939..24493)
                     /locus_tag="OO585_001633"
     CDS             complement(23939..24493)
                     /locus_tag="OO585_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6629259.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6354 a         5913 c         6496 g         6156 t
ORIGIN      
        1 aagccatgct ttgcatccca gaaaaaaagt agggctgata aacaagcgta caaaatcaga
       61 aagggaaggg tagtgtccat aaaacatcct ctgtaattgg agtatcacca tcgaataact
      121 ccggatgctc agcaacagag agagcgctga tttgttaacc tgattccggt gaaaaacgct
      181 atcttcacaa ccttacaaat ttcgtggaat gcagctcgca atctgcgcgt tgcgattaat
      241 ttctacccga tgcttgcgtc aggctcagat ttgcgtataa tgcgcgggct tgtcgtagtt
      301 gacagcaggt tcaatctgaa cctcagttag ccgaatttgg ctacctaaca atgctcccaa
      361 tcggggagct acgtaagaac ggttacactc tcccatcaat cgtaatgggt ctgaggagta
      421 atcattttcg tttataaaat aattggagct ctggtctcat gcagaaccaa agaatccgta
      481 tccgcctgaa agcgtttgat catcgtctga tcgatcaagc aaccgcggaa atcgtcgaga
      541 ctgccaagcg cactggtgcg caggtccgtg gtccgatccc gctgccgaca cgcaaagagc
      601 gcttcactgt tctgatctcc ccgcacgtca acaaagacgc gcgcgatcag tacgaaatcc
      661 gtactcactt gcgtctggtt gacatcgttg agccaaccga gaaaaccgtt gatgctctga
      721 tgcgtctgga tctggctgcc ggtgtagacg tgcagatcag cctgggttaa tcaggtcatt
      781 gagcgattga gaggttgaaa caatgattgg tttagtcggt aaaaaagtgg gtatgacccg
      841 tatcttcaca gaagacggcg tttctatccc agtaaccgta atcgaagttg aagcaaaccg
      901 cgttactcag gttaaagacc tggctaacga tggctaccgt gctattcagg tgaccaccgg
      961 tgctaaaaaa gctaaccgtg tgaccaagcc tgaagctggc cacttcgcta aagctggcgt
     1021 agaagctggc cgtggtctgt gggaattccg cctggctgaa ggcgaagagt tcactgtagg
     1081 tcagagcatt agcgttgaac tgtttgctga cgttaaaaaa gttgacgtaa ctggcacctc
     1141 taaaggtaaa ggtttcgcag gtaccgttaa gcgctggaac ttccgtaccc aggacgctac
     1201 tcacggtaac tccttgtctc accgcgttcc gggttctatc ggtcagaacc agactccggg
     1261 caaagtgttc aaaggcaaga aaatggcagg tcagatgggt aacgaacgtg taaccgttca
     1321 gagccttgac gtagtacgcg ttgacgctga gcgcaacctg ctgctggtta aaggtgctgt
     1381 cccgggtgca accggtagcg acctgatcgt taaaccagct gtgaaggcgt aaggagatag
     1441 caatggaatt agtattgaaa gacgcgcaga gcgcgctgac tgtttccgaa actaccttcg
     1501 gtcgtgattt caacgaagcg ctggttcacc aggttgttgt tgcttatgca gctggtgctc
     1561 gtcagggtac tcgtgctcag aagactcgtg ctgaagtaac tggttccggt aaaaaaccgt
     1621 ggcgccagaa aggcaccggc cgtgcgcgtt ctggttctat caagagcccg atctggcgtt
     1681 ctggtggcgt gacctttgct gctcgtccgc aggaccacag tcaaaaagtt aacaagaaga
     1741 tgtaccgcgg cgcgctgaaa agcatcctgt ccgaactggt acgtcaggat cgtctgatcg
     1801 ttgtcgagaa gttctctgta gaagcgccga aaactaagct gctggcacag aaactgaaag
     1861 acatggctct ggaagatgtg ctgatcatca ccggtgagct ggacgaaaac ctgttcctgg
     1921 ctgcgcgcaa cctgcacaag gttgacgtac gcgatgcaac tggtatcgac ccggttagcc
     1981 tgatcgcctt cgacaaagtc gtaatgactg ctgatgctgt taagcaagtt gaggagatgc
     2041 tggcatgatt cgtgaagaac gtctgctgaa ggtgctgcgt gcaccgcacg tttctgaaaa
     2101 agcgtctact gcgatggaaa aatccaacac catcgtactc aaagttgcta aagacgcgac
     2161 caaagcagaa atcaaagctg ctgtgcagaa actgtttgaa gtcgaagtcg aagtcgttaa
     2221 caccctggta gttaaaggga aagttaaacg tcacggacag cgtatcggtc gtcgtagcga
     2281 ctggaaaaaa gcttacgtca ccctgaaaga aggccagaat ctggacttcg ttggcggcgc
     2341 tgagtaagtc ggaggagtaa tacaatggca gttgttaaat gtaaaccgac atctccgggt
     2401 cgtcgccacg tagttaaagt ggttaaccct gagctgcaca agggcaaacc ttttgctccg
     2461 ttgctggaaa aaaacagcaa atccggtggt cgtaacaaca atggccgtat caccactcgt
     2521 catatcggtg gtggccacaa gcaggcttac cgtattgttg acttcaaacg caacaaagac
     2581 ggtatcccgg cagttgttga acgtcttgag tacgatccga accgttccgc gaacatcgcg
     2641 ctggttctgt acaaagacgg tgaacgccgt tacatcctgg cccctaaagg cctgaaagct
     2701 ggcgaccaga ttcagtctgg cgttgatgct gcaatcaaac caggtaacac cctgccgatg
     2761 cgcaacatcc cggttggttc tactgttcat aacgtagaaa tgaaaccagg taaaggcggt
     2821 cagctggcac gttccgctgg tacttacgtt cagatcgttg ctcgtgatgg tgcttatgtc
     2881 accctgcgtc tgcgttctgg tgaaatgcgt aaagtagaag cagactgccg tgcaactctg
     2941 ggcgaagttg gcaatgctga gcatatgctg cgcgttctgg gtaaagcagg tgctgcacgc
     3001 tggcgtggtg ttcgtccgac cgttcgcggt accgcgatga acccggtaga ccacccacat
     3061 ggtggtggtg aaggtcgtaa ctttggtaag cacccggtaa ctccgtgggg cgttcagacc
     3121 aaaggtaaga agacccgcag caacaagcgt actgataaat tcatcgtacg tcgccgtagc
     3181 aaataatttt agaggataag ccatgccacg ttctctcaag aaaggtcctt ttattgacct
     3241 gcacttgctg aagaaggtag agaaagcggt ggaaagcgga gacaagaagc ccctgcgcac
     3301 ttggtcccgt cgttcaacga tctttcctaa catgatcggt ttgaccatcg ctgtccataa
     3361 tggtcgtcag cacgttccgg tatttgtaac cgacgaaatg gtcggtcaca aactgggtga
     3421 attcgcaccg actcgtactt atcgcggcca cgctgctgat aaaaaagcga agaagaaata
     3481 aggtaggagg aagagatgga aactatcgct aaacatcgcc atgctcgttc ttctgctcag
     3541 aaggttcgcc ttgttgctga cctgattcgc ggtaagaaag tgtcgcaggc tctggatatt
     3601 ttgacctaca ccaacaagaa agcggctgta ctggtcaaga aagttctgga atctgccatt
     3661 gctaacgctg aacacaacga tggcgctgac attgacgatc tgaaagttac gaaaattttc
     3721 gtagacgaag gcccgagcat gaagcgcatt atgccgcgtg caaaaggtcg tgcagatcgc
     3781 atcctgaagc gcaccagcca catcactgtg gttgtgtccg atcgctgaga ctctggagac
     3841 tagcaatggg tcagaaagta catcctaatg gtattcgcct gggtattgta aaaccatgga
     3901 actctacctg gtttgcgaac accaaagaat tcgctgacaa cctggacagc gattttaaag
     3961 tacgtcagta cctgactaag gaactggcta aagcgtccgt atctcgtatc gttatcgagc
     4021 gtccggctaa gagcatccgt gtaaccattc acactgctcg cccgggtatc gttatcggta
     4081 aaaaaggtga agacgtagaa aaactgcgta aggtcgtagc ggacatcgct ggcgttcctg
     4141 cacagatcaa catcgccgaa gttcgtaagc ctgaactgga cgcaaaactg gttgctgaca
     4201 gcatcacttc tcagctggaa cgtcgcgtta tgttccgtcg tgctatgaag cgtgctgtac
     4261 agaacgcaat gcgtctgggc gctaaaggta ttaaagttga agttagcggc cgtctgggcg
     4321 gcgcggaaat cgcacgtacc gaatggtacc gcgaaggtcg cgtaccgctg cacactctgc
     4381 gtgctgacat cgactacaac acctctgaag cgcacaccac ttacggtgta atcggcgtta
     4441 aagtgtggat cttcaaaggc gagatcctgg gtggtatggc tgctgttgaa caaccggaaa
     4501 aaccggctgc tcagcctaaa aagcagcagc gtaaaggccg taaataagga gcgtcgctga
     4561 tgttacaacc aaagcgtaca aaattccgta aaatgcacaa aggccgtaac cgcggtctgg
     4621 cgcagggtac ggatgttagc ttcggcagct tcggtctgaa agctgttggc cgtggtcgtc
     4681 tgactgcccg tcagatcgaa gcagcacgtc gtgctatgac ccgtgcagtt aagcgtcaag
     4741 gtaagatctg gatccgtgtg ttcccggaca aaccgatcac tgaaaagccg ctggcagtgc
     4801 gtatgggtaa aggtaaaggt aacgtggagt attgggttgc cttgattcag ccgggtaaag
     4861 tcctgtatga aatggacggt gttccggaag agctggcccg tgaagcattc aagctggcag
     4921 cagcgaaact gccgattaaa accacctttg taactaagac ggtgatgtaa tgaaagcaaa
     4981 agagctgcgt gagaagagcg ttgaagagct gaacaccgag ctgctgaacc tgctgcgtga
     5041 gcagttcaac ctgcgtatgc aggctgcaag tggccagctg caacagtctc acctgttgaa
     5101 gcaagtgcgt cgcgatgtcg cacgcgttaa gactttactg aacgagaagg ctggtgcgta
     5161 atgaccgata aaatccgtac tctgcaaggt cgcgttgtta gcgacaaaat ggagaaatcc
     5221 attgttgttg ctatcgaacg ttttgtgaaa cacccgatct acggtaaatt catcaagcgt
     5281 acgaccaaac tgcacgtaca tgacgagaac aacgaatgcg gtatcggtga cgtggttgaa
     5341 atccgcgaat gccgtccgct gtccaagact aaatcctgga cgctggttcg cgttgtagag
     5401 aaagcggttc tgtaatacag tacactctct cgatacgaat aaacggctca gaaatgagcc
     5461 gtttattttt tctacccata tccttgaagc ggtgttataa tgccgcgccc tcgatatggg
     5521 gatttttaac gacctgattt tcgggtctca gtagtagttg acattagcgg agcactaaaa
     5581 tgatccaaga acagactatg ctgaacgtcg ccgacaactc cggtgcacgt cgcgtaatgt
     5641 gtatcaaggt tctgggtggc tcgcaccgtc gctacgcagg cgtaggcgac atcatcaaga
     5701 tcaccatcaa agaagcaatt ccgcgtggta aggtcaaaaa aggtgatgtg ctgaaggcgg
     5761 tagtggtgcg caccaagaag ggtgttcgtc gcccggacgg ttctgtcatt cgcttcgatg
     5821 gtaatgcttg tgttcttctg aacaacaaca gcgagcagcc tatcggtacg cgtatttttg
     5881 ggccggtaac tcgtgagctt cgtagtgaga agttcatgaa aattatctct ctggcaccag
     5941 aagtactcta aggagcgaat catggcagcg aaaatccgtc gtgatgacga agttatcgtg
     6001 ttaaccggta aagataaagg taaacgcggt aaagttaaga atgtcctgtc ttccggcaag
     6061 gtcattgttg aaggtatcaa cctggttaag aaacatcaga agccggttcc ggccctgaac
     6121 caaccgggtg gcatcgttga aaaagaagcc gctattcagg tttccaacgt agcaatcttc
     6181 aatgcggcaa ccggcaaggc tgaccgtgta ggctttagat tcgaagacgg caaaaaagtc
     6241 cgtttcttca agtctaacag cgaaactatc aagtaatttg gagtagtacg atggcgaaac
     6301 tgcatgatta ctacaaagac gaagtagtta aaaaactcat gactgagttt aactacaatt
     6361 ctgtcatgca agtccctcgg gtcgagaaga tcaccctgaa catgggtgtt ggtgaagcga
     6421 tcgctgacaa aaaactgctg gataacgcag cagcagacct ggcagcaatc tccggtcaaa
     6481 aaccgctgat caccaaagca cgcaaatctg ttgcaggctt caaaatccgt cagggctatc
     6541 cgatcggctg taaagtaact ctgcgtggcg aacgcatgtg ggagttcttt gagcgcctga
     6601 tcactattgc tgtacctcgt atccgtgact tccgtggcct gtccgctaag tctttcgacg
     6661 gtcgtggtaa ctacagcatg ggtgtccgtg agcagatcat cttcccagaa atcgactacg
     6721 ataaagtcga ccgcgttcgt ggtttggata ttaccattac cactactgcg aaatctgacg
     6781 aagaaggccg cgctctgctg gctgcctttg acttcccgtt ccgcaagtaa ggtagggtta
     6841 ctaaatggct aagcaatcaa tgaaagcacg cgaagtaaaa cgcgtagctt tagctgataa
     6901 atacttcgcg aaacgcgctg aactgaaagc gatcatctct gatgtgaacg cttccgacga
     6961 agatcgttgg aacgctgttc tcaagctgca gactctgccg cgtgattcca gcccgtctcg
     7021 tcagcgtaac cgctgccgtc aaacaggtcg tccgcatggt ttcctgcgga agttcgggtt
     7081 gagccgtatt aaggtccgtg aagccgctat gcgcggtgaa atcccgggtc tgaaaaaggc
     7141 tagctggtaa ttgtcaccaa ttgaatcacg ggagtaaaga cagatgagca tgcaagatcc
     7201 gatcgcggat atgctgaccc gtatccgtaa cggtcaggcc gcgaacaaag ctgcggtcac
     7261 catgccttcc tccaagctga aagtggcaat cgccaacgtg ctgaaggaag aaggttttat
     7321 tgaagatttt aaagttgaag gcgacaccaa gcctgaactg gaacttactc tgaagtattt
     7381 ccagggcaaa gctgttgtag aaagcattca gcgtgtcagc cgcccaggtc tgcgcatcta
     7441 taaacgtaaa gatgagctgc cgaaagttat ggcgggtctg ggtatcgcag ttgtttctac
     7501 ctctaaaggt gttatgactg atcgtgcagc gcgccaggct ggtcttggtg gcgaaattat
     7561 ctgctacgta gcctaatcgg aggaaaaaat gtctcgtgtt gctaaagcac cggtcgttgt
     7621 tcctgccggc gttgacgtaa aaatcaacgg tcaggttatt acgatcaaag gtaaaaacgg
     7681 cgagctgact cgtactctca acgatgctgt tgaagttaaa catgcagata ataccctgac
     7741 cttcggtccg cgtgatggtt acgcagacgg ttgggcacag gctggtaccg cgcgtgccct
     7801 gctgaactca atggttatcg gtgttaccga aggcttcact aagaagctgc agctggttgg
     7861 tgtaggttac cgtgcagcgg ttaaaggtaa tgtgattaac ctgtctctgg gtttctctca
     7921 tcctgttgac catcagctgc ctgcgggtat cactgctgaa tgtccgactc agactgaaat
     7981 cgtgctgaaa ggcgctgata agcaggtgat cggccaggtt gcagcggatc tgcgcgccta
     8041 ccgtcgtcct gagccttata aaggcaaggg tgttcgttac gccgacgaag tcgtgcgtat
     8101 caaagaggct aagaagaagt aaggtaacac tatggataag aaatctgctc gtatccgtcg
     8161 tgcgacccgc gcacgccgca agctccagga gctgggcgca actcgcctgg tggtacatcg
     8221 taccccgcgt cacatttacg cacaggtaat tgcaccgaac ggttctgaag ttctggtagc
     8281 tgcttctact gtagaaaaag ctatcgctga acaactgaag tacaccggta acaaagacgc
     8341 ggctgcagct gtgggtaaag ctgtcgctga acgcgctctg gaaaaaggca tcaaagatgt
     8401 atcctttgac cgttccgggt tccaatatca tggtcgtgtc caggcactgg cagatgctgc
     8461 ccgtgaagct ggccttcagt tctaaggtag aggtgtaaga tggctcacat cgaaaaacaa
     8521 gctggcgaac tgcaggaaaa gctgatcgcg gtaaaccgcg tatctaaaac cgttaaaggt
     8581 ggtcgtattt tctccttcac agctctgact gtagttggcg atggtaacgg tcgcgttggt
     8641 tttggttacg gtaaagcgcg tgaagttcca gcagcgatcc agaaagcgat ggaaaaagcc
     8701 cgtcgcaata tgattaacgt cgcgctgaat aacggcactc tgcaacaccc tgttaaaggt
     8761 gttcacacgg gttctcgcgt attcatgcag ccggcttccg aaggtaccgg tatcatcgcc
     8821 ggtggtgcaa tgcgcgccgt tctggaagtc gctggggttc ataacgttct ggctaaagca
     8881 tatggttcca ccaacccgat caacgtggtt cgtgcaacta ttgatggcct ggaaaatatg
     8941 aactctccag aaatggtcgc tgccaagcgt ggtaaatccg ttgaagaaat tctggggaaa
     9001 taaaccatgg caaagactat taaaattact caaacccgca gtgcaatcgg tcgtctgccg
     9061 aaacacaagg caacgctgct tggcctgggt ctgcgtcgta ttggtcacac cgtagagcgc
     9121 gaggatactc ctgctattcg cggtatgatc aacgcggttt ccttcatggt taaagttgag
     9181 gagtaagaga tgcgtttaaa tactctgtct ccggccgaag gctccaaaaa ggcgggtaaa
     9241 cgcttgggtc gtggtatcgg ttctggcctc ggtaaaaccg gtggtcgtgg tcacaaaggt
     9301 cagaagtctc gttctggcgg tggcgtacgt cgcggtttcg agggtggtca gatgcctctg
     9361 taccgtcgtc tgccgaaatt cggcttcact tctcgtaaag cagcgattac agccgaagtt
     9421 cgtctgtctg acctggctaa agtagaaggc ggtgttgtag acctgaacac gctgaaagcg
     9481 gctaacatta tcggtatcca gatcgagttc gcgaaagtga tcctggctgg cgaagtaacg
     9541 actccggtaa ctgttcgtgg cctgcgtgtt actaaaggcg ctcgtgctgc tatcgaagct
     9601 gctggcggta aaatcgagga ataagtagca gatggctaaa caaccgggat tagattttca
     9661 aagtgccaaa ggtggcttag gcgagctgaa acgcagactg ctgtttgtta tcggtgcgct
     9721 gattgtgttc cgtattggct cttttattcc gatccctggt attgatgccg ctgtacttgc
     9781 caaactgctt gagcaacagc gaggcaccat cattgagatg tttaacatgt tctctggtgg
     9841 tgctctcagc cgtgcttcta tctttgctct ggggatcatg ccgtatattt cggcgtcgat
     9901 cattatccag ctgctgacgg tggttcaccc aacgttggcg gaaattaaga aagaagggga
     9961 gtctggtcgt cgtaagatca gccagtacac ccgctacggt actctggtgc tggcaatatt
    10021 ccagtcgatc ggtattgcta ccggtctgcc gaatatgcct ggtatgcaag gcctggtgat
    10081 taacccgggc tttgcattct acttcaccgc tgttgtaagt ctggtcacag gaacgatgtt
    10141 cctgatgtgg ttgggcgaac agatcactga acgaggtatc ggcaacggta tttcaatcat
    10201 tatcttcgcc ggtattgtcg cgggactccc gccagccatt gcccatacta tcgagcaagc
    10261 gcgtcaaggc gacctgcact tcctcgtgtt gctgttggtt gcagtattag tatttgcagt
    10321 gacgttcttt gttgtatttg ttgagcgtgg tcaacgccgc attgtggtaa actacgcgaa
    10381 acgtcagcaa ggtcgtcgtg tctatgctgc acagagcaca catttaccgc tgaaagtgaa
    10441 tatggcgggg gtaatcccgg caatcttcgc ttccagtatt attctgttcc cggcgaccat
    10501 cgcgtcatgg ttcgggggcg gtactggttg gaactggctg acaacaattt cgctgtattt
    10561 gcagcctggg caaccgcttt atgtgttact ctatgcgtct gcaatcatct tcttctgttt
    10621 cttctacacg gcgttggttt tcaacccgcg tgaaacagca gataacctga agaagtccgg
    10681 tgcatttgta ccaggaattc gtccgggaga gcaaacggcg aagtatatcg ataaagtaat
    10741 gacccgcctg accctggttg gtgcgctgta tattaccttt atctgcctga tcccggagtt
    10801 catgcgtgat gcaatgaaag taccgttcta cttcggtggg acctcactgc ttatcgttgt
    10861 tgtcgtgatt atggacttta tggctcaagt gcaaactctg atgatgtcca gtcagtatga
    10921 gtctgcattg aagaaggcga acctgaaagg ctacggccga taattggtcg cccgagaagt
    10981 tacggagagt aaaaatgaaa gttcgtgctt ccgtcaagaa attatgccgt aactgcaaaa
    11041 tcgttaagcg tgatggtgtc atccgtgtga tttgcagtgc cgagccgaag cataaacagc
    11101 gccaaggctg attttttcgc atatttttct tgcaaagttg ggttgagctg gctagattag
    11161 ccagccaatc ttttgtatgt ctgtgcgttt ccatttgagt atcctgaaaa cgggcttttc
    11221 agcatggaac gtacatatta aatagtagga gtgcatagtg gcccgtatag caggcattaa
    11281 cattcctgat cataagcatg ccgtaatcgc attaacttcg atttatggcg tcggcaagac
    11341 ccgttctaaa gccatcctgg ctgcagcggg tatcgctgaa gatgttaaga tcagtgagct
    11401 gtctgaagga caaatcgaca cgctgcgtga cgaagttgcc aaatttgtcg ttgaaggtga
    11461 tctgcgccgt gaaatcagca tgagcatcaa gcgcctgatg gatcttggtt gctatcgcgg
    11521 cttgcgtcat cgtcgtggtc tcccggttcg cggtcagcgt accaagacca acgcacgtac
    11581 ccgtaagggt ccgcgcaaac cgatcaagaa ataatcgggg tgattgaata atggcaaagg
    11641 caccaattcg tgcacgtaaa cgtgtaagaa aacaagtctc tgacggcgtg gctcatatcc
    11701 atgcttcttt caacaacacc atcgtgacta tcactgatcg tcagggtaac gcgttgggtt
    11761 gggcaacagc cggtggttcc ggtttccgtg gttctcgcaa atccactccg tttgcagctc
    11821 aggttgcagc agagcgttgc gctgacgccg tgaaagaata cggcatcaag aatctggaag
    11881 ttatggttaa aggtccgggt ccaggccgcg aatctactat tcgtgctctg aacgccgcag
    11941 gtttccgcat cactaacatt actgatgtga ctccgatccc tcataacggt tgtcgtccgc
    12001 cgaaaaaacg tcgcgtataa cgcctcgttt tccaggtttg ttggagaaag aaaatggcaa
    12061 gatatttggg tcctaagctc aagctgagcc gtcgtgaggg caccgactta ttccttaagt
    12121 ctggcgttcg cgcgatcgat accaagtgta aaattgaaca agctcctggc cagcacggtg
    12181 cgcgtaaacc gcgtctgtct gactatggtg tgcagttgcg tgaaaagcaa aaagttcgcc
    12241 gtatctatgg tgtgctggag cgtcagttcc gtaactacta caaagaagca gcacgtctga
    12301 aaggcaacac cggtgaaaac ctgttggctc tgctggaagg tcgtctggac aacgttgtat
    12361 accgtatggg cttcggtgcc actcgtgcag aagcacgtca gctggttagc cataaagcaa
    12421 ttatggtaaa cggtcgtgtt gttaatatcg cttcttatca ggttagtccg aatgacgttg
    12481 taagcattcg tgagaaagcg aagaagcagt ctcgcgtgaa agccgctctg gagctggctg
    12541 agcagcgtga aaagccaact tggctggaag ttgatgctgg caagatggaa ggtacgttta
    12601 agcgtaagcc ggagcgttct gatctgtctg cggacattaa cgaacacctg atcgtcgagc
    12661 tttactccaa gtaaagctta gtaccaaaga gaggacacaa tgcagggttc tgtgacagag
    12721 tttctaaaac cgcgcctggt tgatatcgag caagtgagtt cgacgcacgc caaggtgacc
    12781 cttgagcctt tagagcgtgg ctttggccat actctgggta acgcactgcg ccgtattctg
    12841 ctctcatcga tgccgggttg cgcggtgacc gaggttgaga ttgatggtgt actacatgag
    12901 tacagcacca aagaaggcgt tcaggaagat atcctggaaa tcctgctcaa cctgaaaggg
    12961 ctggcggtga gagttcaggg caaagatgaa gttattctta ccttgaataa atctggcatt
    13021 ggccctgtga ctgcagccga tatcacccac gacggtgatg tcgaaatcgt caagccgcag
    13081 cacgtgatct gccacctgac cgatgagaac gcgtctatta gcatgcgtat caaagttcag
    13141 cgcggtcgtg gttatgtgcc ggcttctacc cgaattcatt cggaagaaga tgagcgccca
    13201 atcggccgtc tgctggtcga cgcatgctac agccctgtgg agcgtattgc ctacaatgtt
    13261 gaagcagcgc gtgtagaaca gcgtaccgac ctggacaagc tggtcatcga aatggaaacc
    13321 aacggcacaa tcgatcctga agaggcgatt cgtcgtgcgg caaccattct ggctgaacaa
    13381 ctggaagctt tcgttgactt acgtgatgta cgtcagcctg aagtgaaaga agagaaacca
    13441 gagttcgatc cgatcctgct gcgccctgtt gacgatctgg aattgactgt ccgctctgct
    13501 aactgcctta aagcagaagc tatccactat atcggtgatc tggtacaacg taccgaggtt
    13561 gagctcctta aaacgcctaa ccttggtaaa aaatctctta ctgagattaa agacgtgctg
    13621 gcttcccgtg gactgtctct gggcatgcgc ctggaaaact ggccaccggc aagcatcgct
    13681 gacgagtaac cggatcacag gttaaggttt tactgagaag gataaggtca tgcgccatcg
    13741 taagagtggt cgtcaactga accgcaacag cagccatcgc caggctatgt tccgcaatat
    13801 ggcaggttca ctggttcgtc atgaaatcat caagacgact ctgcctaaag cgaaagagct
    13861 gcgccgcgta gttgagccgc tgattactct tgccaagact gatagcgttg ctaatcgtcg
    13921 tctggcattc gcccgtactc gtgataacga gatcgtggca aaactgttta acgaactggg
    13981 cccgcgtttc gcgagccgtg ccggtggtta cactcgtatt ctgaagtgtg gcttccgtgc
    14041 aggcgacaac gcgccgatgg cttacatcga gctggttgat cgttcagaga aagcagaagc
    14101 tgctgcagag taatctgaag caacgtaaaa aaacccgccc cggcgggttt ttttataccc
    14161 gtagcatccc cacttatcta caatagctgt actctttttg ttcatcccct ggagtattta
    14221 tgtggttact tgaccagtgg gcagagcgtc atatagcaga agcgcaagcg aaaggtgagt
    14281 ttgataatct ggcaggtagc ggcgaaccat tgatactgga tgatgattct catgtgccac
    14341 cggaattacg tgcggggtat cgcttgctga agaatgctgg ttgcttaccg ccagaacttg
    14401 agcagcggag ggaagcaatt cagcttctgg atatcctgag aggtatccgt cacgatgatc
    14461 cgcaatatca agaggttagc cgtcgattgt cattactgga attgaagctg cgacaagctg
    14521 gatttagtac cgatttttta cgcggcgatt atgctgacaa gttgttggac aaaatcaacg
    14581 ataactagtg gagtatgtat gtatcgcatt ggtgagctgg caaaaatggc ggaagtaaca
    14641 cctgacacga ttcgttatta cgaaaaacag cagatgatgg agcatgaagt gcgtactgaa
    14701 ggcgggtttc gcctgtatac cgaaagcgat ctccagcgat tgaaatttat ccgccatgcc
    14761 agacaactag gtttcagtct ggagtcgatc cgcgagttgc tgtcgatccg catcgatcct
    14821 gaacaccata cctgtcagga gtcaaaaggc attgtgcagg aaagattgca ggaagtcgaa
    14881 gcacggatag ccgagttgca gagtatgcag cgttccttgc aacgccttaa cgatgcctgt
    14941 tgtgggaccg cccatagcag tgtttattgt tcgattcttg aagctcttga acaaggggcg
    15001 agtggcgtta agagtggctg ttgatttttt gcactggcag gattacactc gcgccgtaaa
    15061 ataaccaatt ggagttttta tgagccgata tcagcatact aaagggcaga taagggataa
    15121 tgcgatagaa gcattactac atgatccctt attccgacag cgcgtagaga aaaataagaa
    15181 ggggaaaggc agttacatgc gaaaaggaaa acatggcaat cgggggaact gggaggccag
    15241 tggcaagaaa gtaaatcact tttttaccac tggtcttctg ctttcaggcg cttgttaaga
    15301 gcggttattc tgctctttca gcaaatcacg aatttctgcc agtaatactt cttctttagt
    15361 tggtgcaggt gcggcagctg gttcttcttt tttccgattc agtttgttga ttagcttaat
    15421 cgccataaag atggcaaagg ccacaatcag aaaatcaaag acgttttgaa tgaagacacc
    15481 gtaatgcatc acaacagcag ggatatcccc ctgcgcatcg cgtagcgtga cagcaaactg
    15541 tttaaagtcg atcccgccaa ttaataagcc cagaggtggc atgatgatat cggcaaccag
    15601 tgaagataca atcttcccga atgccgcacc gataatgaca cccaccgcca aatccaccac
    15661 gttcccgcgc atcgcaaatt cgcgaaattc tttaataatg ctcatgttat tctccctatg
    15721 aagccgacaa ccataagtct aacaaatgtt aagccatttt cctgccagga atgaatttaa
    15781 ttaatccttg ataatcaaag ggaaataata aggcgtcatt agacgcctta ttaattacaa
    15841 gaagaaaggg cttggctgga agagtctttc gacgtcggta ataaactttt tatctgtgag
    15901 gaacataatt acgtgatcgc cttgctcaat gcgcagattg tcattggcaa tcatcacgtc
    15961 gtttccacgt accaccgctc caataatcgt tcctggcggt agcttgattt cgtcaatgac
    16021 tctgccgaca acgcgtgagg tgctttcatc accgtgagca acggcttcaa tagcttctgc
    16081 tacgccgcgg cgcaatgagg aaacaccaac aatatctgct tttcgcacat ggctaagcaa
    16141 cgcagaaata gttgcttgtt gtggtgaaat tgcaatatcg atcacgctcc cctgaaccag
    16201 atccacataa gcgcgacgct ggatcaatac catcaccttt ttcgcaccca tacgtttggc
    16261 aagcatggcg gacatgatat tggcctcgtc atcgttggtg acggcaataa acagatcaac
    16321 ttgatcgata tgttcttcgg ccagcagttc ttgatccgac gcatcaccaa aaaagacgat
    16381 cgtattctgc aacttttccg ccagttcggc agcgcgctgc tgattacgtt cgatgagttt
    16441 aacgctgtaa tctttttcca gacgacgcgc cagccctgca ccgatattac cgccaccaac
    16501 cagcatgatc cgcttatacg gtttttccag tcgctgtaat tcactcatca ccgcgcggat
    16561 atgctgtgaa gcggcaataa agaacacttc atcaccagct tcaacaatgg tcgaaccttg
    16621 tggacgaatg gggcgatcgt ggcggaaaat tgctgccaca cgagtatcga tatgtggcat
    16681 atgttcgcgc atggtcgaaa gtgcattacc aatcagcggg ccgccataat aggctttaac
    16741 tacagcgagg ctgactttac cctcagcgaa gttcaccacc tgcaatgcgc ctggatactc
    16801 aatcagtcgg taaatattat cgataaccaa ctgctctggt gcgatcagat gatcaatcgg
    16861 tacagcatct gaatgaaata gcttatcggc atcgcgtacg tagtctggtg agcggatgcg
    16921 agcgatgcga ttaggggtgt tgaaaagtga gtaggctacc tggcaggcaa ccatattggt
    16981 ttcatctgaa ctggttacag caaccagcat atcggcgtcg tcggcacctg cctcccgcaa
    17041 tacgcgtgga tgagagccat gcccctgcac gactcgcagg tcaaatttat cctgcaaggt
    17101 ccgcagacgc tcaccgttgg tatcgacaac agtaatatcg ttgttctcgc caaccaggtt
    17161 ttccgccagt gtgccgccaa cctggccggc acccagaatg ataattttca tcagtctcga
    17221 cccgttatct catcactttt tgattagctt agcgtaaaag aagccgtcgc cctcttcggc
    17281 accaggcaga ttttgtttac ccggttgctc tggtgttcct gtttcgcaaa gttcggcatc
    17341 agcggtacgt tgcaaaaaag ctttaatctg caggctattc tcttccggta acaccgaaca
    17401 ggtggcatag accagggttc caccggattt taaatgcggc caaatggcgt cgagaatttc
    17461 agactgcaat tgcgcgagtt ccgggatatc gcgatcgcga cgtaaccatt taatatccgg
    17521 atggtgacga atcacccccg ttgctgaaca aggcgcatct aataaaatgc gatcaaactg
    17581 ttgctcgcca caccattggg aagggtaacg gccatcacct tgtttcacgg ttgccttcat
    17641 accaaggcgt tttaaattgt cgtaaacgcg agagaggcgc tgttcgtcaa tatcaaccgc
    17701 aacaacctgc gcttctggtg ccacctcaag gatatgcgtt gttttaccgc cgggggcggc
    17761 acaaagatcc agaatgtgtt caccgttttg tggcgcaagc caggtcatgc aaccttgtgc
    17821 tgatgcatcc tgaacggtaa cccatccgtc ttcaaaacca ggtagcgcat gaacaggtgc
    17881 aggtgtttcc agacgtacag catcagggta atccgcatgc gggaaacctt tcattcctgc
    17941 ttcatccagc aatgcaagcc agctgtcgcg ggaatgatgc gtacgattaa cgcgcagcca
    18001 cattggcgga cgctggttat tggcttcgac gatggattgc cactgctctg gatacgcttt
    18061 ttgcagacgc ttcagcaacc aggaaggatg cagataacgt gcatcactgg cattaaactt
    18121 ggctaataac tcttcttgct gacgctggaa ctggcgtaat acaccgttaa tcaacccttt
    18181 aagttgcgga cgcttaattg cgatagcgcc ttcaaccgtt tcagccagcg cagcatgagg
    18241 tggaatgcgg gtataaagca gttgatacaa accaaccata atcaggtaat gcacagtgcg
    18301 ctgtttgccg gtcatcggac gggccattaa cttattaatc agccagtcta actgcgaaag
    18361 cgtacgcagt acgccaaagc acaactcttg aagaagtgct ttgtctttat cagaaacttt
    18421 ttgctggagc ggtggcagaa tgttgcttaa tgattgccct tgctcgacga cttgttcaac
    18481 ggcctgggcc gccatgctac gtaaattacg ttgttttttc ataagtataa aaatgcccgg
    18541 taagaccggg cttagaagag tggactatca ggccagacgg ttgcccggaa caaaccattc
    18601 ccgacgagaa ttcaggaggt cttgcgcgct catcgctttc ttacccgcag gttgtaacga
    18661 gagcagattc aggattccat caccagttgc aacctgaatg ccttgtttgt tggcttcaag
    18721 gatcgttcct ggtgcagcgt tggttgccgt atcaatgacc gacgctttcc agactttaac
    18781 cggctgtctt tcaatttcca gccagctcat tggccatgga ttgaaagcgc gaatgtagcg
    18841 ttcaagctgt gctgccgaaa gtgaccagtc aatacgcgct tcttctttac tcaacttttc
    18901 ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc gccgtgccgt ctgccagttg
    18961 tttcaacgtg gtgataagcc cttgtgggcc aagctctgcc agcttgtcgt acagcgtacc
    19021 actggtatct tctgcagtaa tcgggcagga gagcttatag agcatgtcac cggtgtctaa
    19081 accgacatcc atttgcataa tggtcacacc agtttctgca tcacccgccc atagtgagcg
    19141 ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa ccatgaacgt tgatacagcc
    19201 aagacgcggc atctccagca ctgctttagg cagaattaag ccataggcga cgacgaccat
    19261 aacatcagcc tgcagatcgg cgaccagttg ctggttttct tgtggacgca gggaaacagg
    19321 ttgaaaaacg ggcagacctt tttcctcagc cagaacttta accgggctgg gcatcagttt
    19381 tttaccgcgt cctgccggtc ggtctggctg ggtgaacacg ccaacgacgt tatgaccaga
    19441 agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt gtacccgcaa aaataatacg
    19501 tagtgattct gacacgttag ttcttatcct taagcccggg ctttcagacg atccagtttt
    19561 tcaactttct gacgaatacg ttgttgtttc aacggtgaca gataatccat aaacagtttg
    19621 ccgaccaggt gatccatctc atgctgaata cagatggcta acagaccgtc tgcttccaat
    19681 tcaaatggtt taccgtcgcg gtccagggcg cgaattttaa ctttctctgc gcgtggcact
    19741 aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa tgcctgtttc gccgcttttt
    19801 tctaaaagct ccggattgat taacaccagc cgttcgtcac ggttttccga aacatcaata
    19861 acaatgatac gttgatggat atcaacctgg gttgccgcca ggccaatacc ttcttctgcg
    19921 tacatcgtct cgaacatatc atcgacgata cgctgaattt ctgcattcac ttcttctacc
    19981 ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta acacttgcaa aactgacata
    20041 aatctccaga gatgtgttca ggagttagaa agattatttc ttctattcta gacaaatccc
    20101 cctctgattg acagcatcac tgaccaatcg caaagattgc taaggctgct tatggcaggg
    20161 agataaggat ggtcgataca gatatttggc tgcgtttaat gagcatcagc agcttgtacg
    20221 gcgatgatat ggtccgtata gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
    20281 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccgcgaa
    20341 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
    20401 acagcgaatt ttatcctcct caacttctgg cgacgacaga ttaccccggc gcactgtttg
    20461 ttgaaggaga actgtacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
    20521 attcatggta tggcgagcga tggggacgat tattttgcga aactctggcc acgcgtggag
    20581 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggcgcataaa gcggccttac
    20641 aggtaaatgg cgtcagcatt gctgtattgg ggaacggact taataccatt catccgcgcc
    20701 gccatgcccg actggctacc agtttgcttg aacatggtgg ggcacttgtc tcggaatttc
    20761 ccctcgatgt ttcacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
    20821 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgttg gtgacagcac
    20881 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggaccaata gggaatccgg
    20941 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
    21001 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
    21061 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
    21121 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
    21181 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
    21241 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
    21301 attcacacag aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
    21361 gcaggatttg atcgagaaga tatctacaat gccctgcttt ggctggaaaa gcttgcggat
    21421 tatcaggaag gactgacaga accgatgcaa ctggcctctg atccactctc catgcgtatt
    21481 tatacccctg aggagtgtga aagactggat gccagctgtc gtggttttct gcttttcctt
    21541 gagcaaattc aggtgcttaa ccttgaaatc cgtgaaatgg tgatagagcg agtgctcgcg
    21601 ctggataccg cagagttcga cctggaagac ctgaaatggg taatcctgat ggtgttgttc
    21661 aatattccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
    21721 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg cgaaatcagc
    21781 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
    21841 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
    21901 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
    21961 tgcatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
    22021 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
    22081 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
    22141 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac ccatagctgg
    22201 agaatgccct gagtgtcatt atccactact catcgaaaag aaaaccgcgc agggtgtaaa
    22261 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
    22321 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa gaacgtgtca
    22381 tcgcctatcc aacggaagcc gttttcggtg ttgggtgcga tcctgatagc gaaacagcag
    22441 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
    22501 caaattacga gcagcttaaa ccctatattg atgacaccat gctgactgat gcgcagcgtg
    22561 aaaccatttt ttcccgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
    22621 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
    22681 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
    22741 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
    22801 cggttgtgct tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
    22861 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
    22921 atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcagcaact gaatattgaa
    22981 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
    23041 tttagtgctg gtggtaaagg tgcgaatgtg acggtacctt ttaaagaaga ggcttttgcc
    23101 agagcggatg agcttactga acgggcagcg ttggctggtg ctgttaatac cctcaagcgg
    23161 ttagaagatg gacgcttgca gggtgacaat accgatggta taggtttgtt aagcgatctg
    23221 gaacgtctgt cttttatccg tccgggttta cgtattctgc ttatcggcgc tggtggagca
    23281 tctcgcggcg tactactacc actcctttcc ctggactgtg cggtgacaat cactaatcgg
    23341 acggtatccc gtgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
    23401 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
    23461 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatctagg catttattgc
    23521 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
    23581 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
    23641 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
    23701 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
    23761 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
    23821 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
    23881 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
    23941 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
    24001 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
    24061 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
    24121 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
    24181 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
    24241 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
    24301 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
    24361 tcacaacgag cggccagatc cccacatcat cagccagacg aacgccacca atcacgacac
    24421 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
    24481 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
    24541 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
    24601 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
    24661 ggatccctat aatgcgcctc cgttaagacg acaacgtgaa acacttcaca ggatggtcgg
    24721 aacaacgaag agaaaaaatc ctgaaattca gggttgactc tgaaagagga aagcgtaata
    24781 tacgccacct cgcgacagtg agctgaaagc cgcgtcgcaa ctgctcttta acaatttatc
    24841 agacaatctg tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa
    24901 taccaagtct caagagtga
//
LOCUS       DAJTYD010000044        24408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTYD010000044 DAJTYD010000000
VERSION     DAJTYD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.044"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..537)
                     /gene="maa"
                     /locus_tag="OO585_001634"
     CDS             complement(<1..537)
                     /gene="maa"
                     /locus_tag="OO585_001634"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6629260.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARI"
     gene            complement(709..927)
                     /gene="hha"
                     /locus_tag="OO585_001635"
     CDS             complement(709..927)
                     /gene="hha"
                     /locus_tag="OO585_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6629261.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(953..1327)
                     /gene="tomB"
                     /locus_tag="OO585_001636"
     CDS             complement(953..1327)
                     /gene="tomB"
                     /locus_tag="OO585_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6629262.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(1873..5022)
                     /locus_tag="OO585_001637"
     CDS             complement(1873..5022)
                     /locus_tag="OO585_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6629263.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5045..6238)
                     /gene="acrA"
                     /locus_tag="OO585_001638"
     CDS             complement(5045..6238)
                     /gene="acrA"
                     /locus_tag="OO585_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6629264.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6380..7027
                     /gene="acrR"
                     /locus_tag="OO585_001639"
     CDS             6380..7027
                     /gene="acrR"
                     /locus_tag="OO585_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6629265.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7155..10517
                     /gene="mscK"
                     /locus_tag="OO585_001640"
     CDS             7155..10517
                     /gene="mscK"
                     /locus_tag="OO585_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6629266.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(10729..10890)
                     /gene="rsmS"
                     /locus_tag="OO585_001641"
     CDS             complement(10729..10890)
                     /gene="rsmS"
                     /locus_tag="OO585_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6629267.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(10904..11431)
                     /gene="priC"
                     /locus_tag="OO585_001642"
     CDS             complement(10904..11431)
                     /gene="priC"
                     /locus_tag="OO585_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6629268.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11501..11878
                     /locus_tag="OO585_001643"
     CDS             11501..11878
                     /locus_tag="OO585_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6629269.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12031..12582
                     /gene="apt"
                     /locus_tag="OO585_001644"
     CDS             12031..12582
                     /gene="apt"
                     /locus_tag="OO585_001644"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6629270.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            12711..14582
                     /gene="dnaX"
                     /locus_tag="OO585_001645"
     CDS             12711..14582
                     /gene="dnaX"
                     /locus_tag="OO585_001645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6629271.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    13975..14039
                     /gene="dnaX"
                     /locus_tag="OO585_001645"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            14635..14964
                     /locus_tag="OO585_001646"
     CDS             14635..14964
                     /locus_tag="OO585_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6629272.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            14964..15569
                     /gene="recR"
                     /locus_tag="OO585_001647"
     CDS             14964..15569
                     /gene="recR"
                     /locus_tag="OO585_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6629273.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            15679..17553
                     /gene="htpG"
                     /locus_tag="OO585_001648"
     CDS             15679..17553
                     /gene="htpG"
                     /locus_tag="OO585_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6629274.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            17734..18378
                     /gene="adk"
                     /locus_tag="OO585_001649"
     CDS             17734..18378
                     /gene="adk"
                     /locus_tag="OO585_001649"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6629275.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            18510..19472
                     /gene="hemH"
                     /locus_tag="OO585_001650"
     CDS             18510..19472
                     /gene="hemH"
                     /locus_tag="OO585_001650"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6629276.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19469..20428)
                     /gene="aes"
                     /locus_tag="OO585_001651"
     CDS             complement(19469..20428)
                     /gene="aes"
                     /locus_tag="OO585_001651"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6629277.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            20580..21884
                     /gene="gsk"
                     /locus_tag="OO585_001652"
     CDS             20580..21884
                     /gene="gsk"
                     /locus_tag="OO585_001652"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6629278.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22017..23693)
                     /locus_tag="OO585_001653"
     CDS             complement(22017..23693)
                     /locus_tag="OO585_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6629279.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(23931..>24408)
                     /gene="fsr"
                     /locus_tag="OO585_001654"
     CDS             complement(23931..>24408)
                     /gene="fsr"
                     /locus_tag="OO585_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6629280.1"
                     /translation="YYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGR
                     KYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGM
                     VSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6074 a         6382 c         6277 g         5675 t
ORIGIN      
        1 tattctggct ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc
       61 acctgaagct accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc
      121 gccaatccag acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg
      181 tgctacaggg tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca
      241 gttatcaccg atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa
      301 attattgccg agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc
      361 ctctgtcacc tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc
      421 cagggaatga ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt
      481 ctcatctgcc gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac
      541 tgacctccgg ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaacagaacg
      601 ttcccattac atctgtgtga taaagatcac atagggtatg tcttcatggt gaaaaagtat
      661 aaaattctta ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa
      721 tttccatact gaggaaggga tcttgtcgta tagtttattc atggtcaatt cggcgaggcg
      781 gtgatctgcg gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt
      841 ctcgataaca cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc
      901 ggttttcgtt aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag
      961 ataaactcgc aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg
     1021 acttccgcca tttctgaaga tcctgcatat taataccata actactgaac aacataaagg
     1081 tgtcatccag atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt
     1141 tgtaattaag tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg
     1201 ccgaggttgg gtcgttaacc cagccataat tgctttcttc aaggtttgca aggcagtcat
     1261 gatacagggt ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt
     1321 catccataac gcgtcccctt cttagcggtt gaactaacgg acacctttcg ggatggaaaa
     1381 aacttactga cctggacttg ccctctcgtt tgttagctta actataagcc actctttgca
     1441 ggtattcatc gcattattac gaaaaattac aattaggaag gaatcgggac aggactcact
     1501 ttttcatact agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca
     1561 tttcaggatt atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga
     1621 cgtaataacc gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg
     1681 attatggatt attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt
     1741 tttatgaatt ttatccgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc
     1801 aattactcct taatgttcgt aggttatgca taaaaaaggc cgcttgcgcg gccttagtga
     1861 ttacacgttg tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg
     1921 gctaaagcgg cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt
     1981 tgcggtcacc atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc
     2041 accagtactg ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat
     2101 cggacgtaaa cgcatccgca ctgcatcaag cgtcgcttca atcagacctt taccttcttt
     2161 atccatcaag tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat
     2221 ggttgtgagc aggcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc
     2281 cagcaacgca ccgataaccc ccagcggaac gaccagcata acggagaacg gaatcgacca
     2341 gctctcgtac agcgccgcta gacacaggaa tacgacaatc aacgaaatcg cgtacagtga
     2401 aggtgcctgg ttgccggaga ggcgttcctg ataggacatc cccgtccagt catagccaac
     2461 accggtaggc agtttgctcg ccagttgttc cattagctcc attgcttcac cggtactttt
     2521 acccggtgcc gcctggccta agatttccat ggatggcagg ccattgtaac gttccagacg
     2581 cggcgaaccg tactcccaac gagaagagga gaatgccgag aacggcacca tctgaccatc
     2641 agcagcacga acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga
     2701 catgacgtaa actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc
     2761 ccatgcagcg cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt
     2821 ttcctggtcg atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct
     2881 ggtcaacata tcaggatgct ttgctgcttc tgcaagcagc tggttacgcg cctgagtcag
     2941 tttttcgtga ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc
     3001 cagttccacg attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa
     3061 agcgcgtgtt gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc
     3121 ccagtccttc aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa
     3181 gccgttaacg gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt
     3241 tacctcattg agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat
     3301 ggtcataaat acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag
     3361 ataggccatg ccgaccacga tgatcagata cagcaccagg taacgccccg tactgcgcag
     3421 aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca
     3481 gccgaagaag ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt
     3541 ggcacaaaga gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac
     3601 aatggtaata gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg
     3661 tacgaatacc gctgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat
     3721 cgacttacgg gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt
     3781 ttctaccaca acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat
     3841 tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac
     3901 caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat
     3961 aaccaggaac acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat
     4021 tttcacgaac ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg
     4081 ttccatcttc gccagttcag cacggattgc cgcagcggta tccagcgcat ttgcaccggt
     4141 cgccagcttg atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta
     4201 gttctcacca cccagctcaa tcttcgccac atcacgcagc aggacgcggg aaccatcctg
     4261 attcactttc agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat
     4321 aatagaggcg ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc
     4381 aacctgggcg ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt
     4441 gttcagctca ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac
     4501 atcacccacg cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga
     4561 gatatcctcc tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct
     4621 gctggatgat ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat
     4681 cgccagctgc agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc
     4741 aaaggtcagg gtgatctgca cggtccccgt ggagtcactg ttagaggaca tgtacatcag
     4801 gttatcgata ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt
     4861 cgcatcagcg ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata
     4921 ttgcgccacc ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat
     4981 cacccacgca aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt
     5041 taagttaaga cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag
     5101 cggtaacttc ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta
     5161 ctacgcgatc gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc
     5221 ttgcaacgat cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat
     5281 caccacgcgg cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc
     5341 cttcttccag acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga
     5401 agatagcgcg tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca
     5461 gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc
     5521 cgttctcttg tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc
     5581 tggactgggt cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg
     5641 cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa
     5701 tcggagcggt gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg
     5761 ccgcagttac cgcagcattc gcctgttgcg catcagccag agcctgatcg tactcttgct
     5821 tactgatgta ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat
     5881 tggctgcagc ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg
     5941 tcgcaggatc aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat
     6001 tacgcttcag gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg
     6061 tgcgacccgg aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa
     6121 cggcgggcat ctgctggcca ccttgttggg cctgtttgtt gtcacatcct gttagggcta
     6181 agctgcctga gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat
     6241 gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc
     6301 tgcgtttata ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg
     6361 cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca
     6421 acacatcctc gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct
     6481 gggtgagatt gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga
     6541 caagtcggat ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga
     6601 gcttgagtat caggcaaaat tccctggcga tccactctca gtattaagag agatattaat
     6661 tcatgttctt gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt
     6721 ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg
     6781 tctggaaagt tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt
     6841 gcctgcggat ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct
     6901 gatggaaaac tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta
     6961 cgtcgccatc ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa
     7021 cgaataaccc tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg
     7081 gttcactgcg tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga
     7141 aaccttcatt catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt
     7201 cagcatttat tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt
     7261 catcgaatgg tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata
     7321 aacaaaaaga tctttctgct caggacaaac tggagctgca ggatctgaca gatacattag
     7381 ccaccctcga taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg
     7441 ctgaagcgcc ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg
     7501 ataacgacga agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc
     7561 gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca
     7621 gccagctggt ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt
     7681 cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct
     7741 tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg
     7801 accagcagcg taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac
     7861 gtgattacgt gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag
     7921 cggtaaacag caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg
     7981 atgaagccgc gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc
     8041 agttaagtca gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca
     8101 ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga
     8161 ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc
     8221 tgccctcggc ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac
     8281 agtttgaagt taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac
     8341 tggaagaagg tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg
     8401 ttgatatgcg tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga
     8461 tggccattaa cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat
     8521 ccatcctgac tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga
     8581 tcaaagcgtt cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact
     8641 gggaaaaagc ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga
     8701 ttgtcgggct gatccactgg cgtctgggct ggctgaaagc gtatcaacaa aaactggctt
     8761 ccgctgtggg ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg
     8821 acctgatccg cgcgctgccg gtgtgcctga ttattctcgc ggttggcttg attctgttga
     8881 ccatgcagct caacatcagc gaactgctat ggtcgttcag caaaaaactg gcgattttct
     8941 ggctggtgtt tggcctgtgc tggaaggtac tggagaagaa cggcgttgcc gtgcgtcact
     9001 tcggcatgcc ggaacagcag accagccact ggcgtcggca aattgtccgc atcagtctcg
     9061 cattgctgcc tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg
     9121 atgatgtgct ggggcaagcg atgattttct tcaacctgct gctgattgcc ttcctggtat
     9181 ggccaatgtg ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctggtcacca
     9241 ttaccgtgct gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct
     9301 acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga
     9361 acttgctcta ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc
     9421 gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc
     9481 cggaagaacc caccattgca ctggaacagg ttaaccagca gacgctgcgt attaccatgt
     9541 tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg
     9601 tgttcagcta tatcgacagc attacgctct ggcattacaa cggtactgaa gctggcgctg
     9661 cggtggtgaa aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg
     9721 cctgggcgtt gattcgcaac ctgcctggtt tgctggaagt gctggtgctc tcgcgactga
     9781 atatgcgcca gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg
     9841 ttggtgcgat gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg
     9901 cggcagcatt atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct
     9961 ccggtttgat cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta
    10021 gcttctcggg gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc
    10081 gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt
    10141 tgactgacac tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg
    10201 aaaaagtgcg taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac
    10261 caatgccgga agtcttcttt acggcgtttg gtgccagcac gttggatcat gagctgcgtc
    10321 tgtatgtgcg tgaactgcgt gaccgtagtc gtactgtcga tgagctgaac cgtactatcg
    10381 atcagctgtg ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc
    10441 acaacgagaa gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gatgacccga
    10501 cgccagcggt agggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca
    10561 aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca
    10621 agaattcaat gtattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt
    10681 tgcgtttgcc cacaatctta ggggacattt agcggcccca tttatttctc acttttccgc
    10741 ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt acaatatcca gcgcgcgcag
    10801 cacggtgcgg gctgggatct gattttcttc cagcagcaca atcaaatcga cagccagttt
    10861 gacatcgtca ggggcatttt ccagtgacat actctctcca ttgctagcgg gttaagcgcg
    10921 ctagcctgtt ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg
    10981 cttccacttc acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg
    11041 ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt
    11101 tacgctgcca gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg
    11161 aggcttcacg cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt
    11221 gcggcagctg ttgctgctca actgcatgac gaagcgcagc cagattatcg ccagcctcat
    11281 cgagacaagc ctgcagtgtt gtcgtacggg tttgaaaaag gtgtctgtca aaacgggcac
    11341 ttagcgtggc aaattgcgct accggggcac aacgctgacg cagcgtagcg agctgtcctt
    11401 ccagtttttc cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt
    11461 tatgctaaag tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat
    11521 catcattggc tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc
    11581 gacgacgccg tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca
    11641 cgcctggttg ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca
    11701 tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat
    11761 ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg
    11821 tttgcttttc tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag
    11881 cacaacaatc gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac
    11941 aggcgcaggc ggtcaaaggt taaacaactg ttacttttga tacgtttaaa acgcgccgtg
    12001 agtaccaccg taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat
    12061 ctcaaaaata gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat
    12121 gtcaccagct tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag
    12181 cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt
    12241 ggcgctccgg tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa
    12301 ctgccgcgtg aaaccatcag tgaaacttac gacctggaat acggcaccga tcagctggag
    12361 atccacgttg atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca
    12421 accggcggca ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct
    12481 gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag
    12541 ggcattacca gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc
    12601 tgcccacgct acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg
    12661 ttagcattac cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc
    12721 aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg
    12781 tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt
    12841 ccggtacccg tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact
    12901 gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc
    12961 aggggcgctt tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata
    13021 cccgcgacct gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc
    13081 tgatcgacga agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg
    13141 aagagccgcc ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg
    13201 tgacgatttt gtcgcgctgt ttgcaatttc atctcaaggc gctggatgtc gagcaaattc
    13261 gccatcaact tgagcacatc ctcaacgaag aacatatcgc tcacgagccg cgggcgttgc
    13321 aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg
    13381 cgattgccag cggtgacggc caggtttcaa cccaggcagt cagtgcgatg ctgggtacgc
    13441 ttgatgacga tcaggcgctg tcgctggttg aagcgatggt cgaggccaac ggcgagcgcg
    13501 taatggcgct gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg
    13561 aaatgctcgg cctgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca
    13621 acgacatggc cgccattgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg
    13681 atattcagct ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg
    13741 accgccgcat gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccacgtatgc
    13801 ctctgcctga gccagaagtg ccgcgccagt cctttgcacc cgtcgcgcca acggcagtaa
    13861 tgacgccaac ccaggtgccg ccgcaaccgc aatcagcgcc gcagcaggca ccgactgtac
    13921 cgctcccgga aaccaccagc caggtgctgg cggcgcgcca gcagttgcag cgcgtgcagg
    13981 gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac ccgcgcgcgt ccggtgccat
    14041 cggcgctgga aaaagcgcca gccaaaaaag aagcgtatcg ctggaaggcg accactccgg
    14101 tgatgcagca aaaagaagtg gtcgccacgc cgaaggcgct gaaaaaagcg ctggaacatg
    14161 aaaaaacgcc ggaactggcg gcgaagctag cggcagaagc cattgagcgc gacccgtggg
    14221 cggcacgggt gagtcaactt tcgctaccaa aactggtcga acaggtggcc ttaaatgcct
    14281 ggaaagagga gagcgacaac gcagtatgtc tgcatttgcg ctcctctcag cggcatttga
    14341 acaaccgcgg tgcacagcaa aaactggctg aagcgttgag cacgttaaaa ggttcaacgg
    14401 ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg tacgccgctg gaatggcgtc
    14461 aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc cattattgcg gataataata
    14521 ttcagaccct gcgtcggttc ttcgatgcgg agctggatga agaaagtatc cgccccattt
    14581 gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat tgagagagaa acctatgttt
    14641 ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc agatgcaaga aaaaatgcag
    14701 aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg aatctggcgc aggtctggta
    14761 aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag agatcgaccc gagcctgctg
    14821 gaagacgaca aagagatgct ggaagacctg gtggctgcag cattcaacga cgcagcacgt
    14881 cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat cctccggaat gcagctgccg
    14941 cctggcttta agatgccgtt ctgatgcaaa ccagcccgct gttaacacag cttatggaag
    15001 cactgcgctg tctgtcgggc gttggcccga agtcggcgca gcgtatggcg ttcacgctgc
    15061 ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc gctcacccgg gcgatgtcgg
    15121 aaatcggcca ctgcgccgat tgccgcactt tcaccgaaca ggaagtctgt aacatctgtt
    15181 cgaatccgcg tcgtcaggaa aacggtcaaa tctgtgtggt ggagagtccg gcggacatct
    15241 acgccattga gcagacgggg cagttttctg gtcgttattt tgtgttgatg gggcatctgt
    15301 caccgctgga cggcatcggt ccggatgata tcgggcttga tcgtctggaa cagcgtctgg
    15361 cagaggaaaa aatcactgaa gtgatcctcg ccaccaatcc cacggttgaa ggtgaagcta
    15421 ccgctaacta cattgccgag ctttgcgcgc aatatgacgt ggaagctagc cgaatcgctc
    15481 atggcgtacc ggttggcggc gaactggaaa tggtcgatgg caccacgctg tcacactccc
    15541 ttgccgggcg tcataagatt cgtttttaag caaacgagag caggatcacc tgctctcgct
    15601 tgaaattatt ctcccttgtc cccatctctc cctcatcctg tttttaacct taaaatggca
    15661 ttattgaggt agacctacat gaaaggacaa gaaactcgtg gttttcagtc agaagtgaaa
    15721 cagcttctgc acctgatgat ccattctctc tattccaata aagaaatctt cctgcgtgag
    15781 cttatctcta acgcctccga tgcggcggac aagctgcgtt tccgtgcgct atctaacccg
    15841 gacctgtacg aaggtgatgg cgaactgcgc gttcgtgtct ctttcgataa agacaagcgc
    15901 acgctgacca tctccgataa cggcgtgggg atgacccgcg acgaagtgat tgaccatctg
    15961 gggactatcg ctaaatccgg taccaaatca ttcctcgaat ccctgggttc tgaccaggcg
    16021 aaagacagcc agctgatcgg tcagtttggt gttggtttct actctgcgtt tatcgtggcc
    16081 gacaaagtga ctgtgcgtac tcgcgcggca ggcgaaaaac cagaaaacgg cgtcttctgg
    16141 gaatcggctg gcgaaggtga atataccgtt gccgacatca ccaaagaaga tcgtggtact
    16201 gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc tcgatgactg gcgcgtgcgt
    16261 tccatcatca gcaaatactc cgaccatatc gcgctgccgg tagagatcga aaaacgcgaa
    16321 gagaaagacg gcgaaaccgt tatctcctgg gagaaaatca acaaagcgca ggcgctgtgg
    16381 actcgtaaca agtcggaaat caccgatgaa gagtacaaag agttctacaa acacatcgcc
    16441 cacgacttta atgatccgct gacctggagc cacaaccgcg ttgaaggtaa gcaggagtac
    16501 accagcctgc tgtacatccc gtcccaggct ccgtgggata tgtggaaccg tgatcataaa
    16561 cacggcctga aactgtacgt ccagcgtgtg ttcatcatgg acgacgcaga acagttcatg
    16621 ccgaactatc tgcgcttcgt gcgtggtctg attgactcca gcgatctgcc gctgaacgtt
    16681 tcccgtgaaa tcctccagga cagcacggta acgcgtaacc tgcgcaatgc gctgaccaag
    16741 cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg cggaaaaata ccagaccttc
    16801 tggcaacagt ttggcctggt actgaaagaa ggtccggcgg aagatttcgc taaccaggaa
    16861 gcgatcgcca aactgctgcg ttttgcttct acccataccg attcttctgc gcagaccgta
    16921 tctctggaag actacgtttc ccgcatgaaa gaagggcagg agaaaatcta ctacatcacc
    16981 gcagacagct atgcggcagc gaagagcagc ccgcacctgg aactgctgcg taagaaaggc
    17041 atcgaagttc tgctgctttc cgaccgcatc gatgagtgga tgatgaacta tctgactgag
    17101 ttcgacggta aaccgttcca gtcggtgtct aaagttgacg agtcgctgga aaaactggct
    17161 gacgaagttg atgagagcgc gaaagaagcg gagaaagcac tgactccgtt catcgaccgt
    17221 gtgaaagcct tgctcggcga gcgcgtgaaa gatgtccgtc tgactcaccg tctgaccgat
    17281 acgccagcga tcgtttcgac cgacgcggac gaaatgagca ctcagatggc gaaactgttc
    17341 gctgcggcgg gccagaaagt gccagaagtg aaatacatct tcgaactgaa cccggatcac
    17401 gtactggtga aacgtgcggc agatactgaa gatgaagcga agttcagcga gtgggtagaa
    17461 ctgctgctgg atcaggcgct gctggcagaa cgcggcacgc tggaagatcc gaacctgttt
    17521 attcgtcgta tgaaccagct gctggtttcc tgatgtcatg ccggatgacc tttgtgtcat
    17581 ccggcatttt tcttttcatc atctgcactt tccgcaaatt atctcgccat taaccgtttc
    17641 agcctcaggt gcctttcttg aggcaatcgc ctgttggtgg tatcgtttat cgctttttca
    17701 aaaaattcga cacattttaa ggggattttc gcaatgcgta tcattctgct tggcgctccg
    17761 ggcgcgggga aagggactca ggctcagttc atcatggaga aatatggtat tccgcaaatc
    17821 tccactggcg atatgctgcg tgctgcggtc aaatctggct ccgagctggg taaacaagca
    17881 aaagacatta tggatgctgg caaactggtc accgacgaac tggtgatcgc gctggttaaa
    17941 gagcgcattg ctcaggaaga ctgccgtaat ggtttcctgt tggacggctt cccgcgtacc
    18001 attccgcagg cagacgcgat gaaagaagcg ggcatcaatg ttgattacgt tctggaattc
    18061 gacgtaccgg acgaactgat tgttgatcgt atcgtaggcc gccgcgttca tgcgccgtct
    18121 ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag aaggcaaaga cgacgttacc
    18181 ggtgaagaac tgactacccg taaagacgat caggaagaaa ccgtacgtaa acgtctggtt
    18241 gaataccatc agatgactgc accgctgatc ggctactact ccaaagaagc ggaagcgggt
    18301 aacaccaaat acgcgaaagt tgacggcacc aagccggttg ctgaagttcg cgctgatctg
    18361 gaaaaaatcc tcggctaatc aagccgcagc agatctgctt tcgggcaggt ctgctcatcc
    18421 acctcaggca atacctctca cagcaattag ttcttcttcc tcacttttcc gctacaatta
    18481 tcaacaagtt gaatcgataa gaggcggtaa tgcgtcagac taaaaccggt atcctgctgg
    18541 caaacctggg tacgcccgat gcccccacac ctgaagcggt aaaacgctat ctgaaacaat
    18601 ttttaagcga cagacgcgtg gttgatacct cacggttgtt atggtggcca ttgctgcgcg
    18661 gcgttatttt gccgctgcgc tcgccgcgtg tggcgaagct gtatgcctct gtctggatgg
    18721 aaggtggctc gccgctgatg gtttacagcc gccagcaaca gcaggcgctg gcacaacgtt
    18781 taccggagac gcccgtagcg ctgggaatga gctacggctc gccatcactg gaaagcgccg
    18841 tcgatgaact cctggcagag catgtagatc atattgtggt gctgccgctt tatccgcaat
    18901 tctcctgttc tacggtcggt gcggtatggg atgaactggc acgcattctg gcgcgcaaac
    18961 gtagcattcc ggggatatcg tttattcgtg attacgccga taaccacgat tacattaatg
    19021 cactggcgaa cagcgtacgc gcttcttttg ccaaacatgg cgaaccggat ctgctgctgc
    19081 tctcttatca tggcattccc cagcgttatg cagatgaagg cgatgattac ccgcaacgtt
    19141 gccgcacaac gactcgcgaa ctggcttccg cactggggat ggcaccggaa aaagtgatga
    19201 tgacctttca gtcgcgcttt ggccgggaac cctggctgat gccttatacc gacgaaacgc
    19261 tgaaaatgct cggagaaaaa ggcgtaggtc atatacaggt gatgtgcccg ggctttgctg
    19321 cggattgtct ggagacgctg gaagagattg ccgagcaaaa ccgtgaggtc ttcctcggtg
    19381 ccggcgggaa aaaatatgaa tatattccag cgcttaatgc cacgccagaa tatatcgaaa
    19441 tgatggctaa tcttgttgcc gcgtatcgct aaagctgagc ggtaaagaac tgagcgccgt
    19501 cgcgaagagc ctcgtcggcg gttttcatca ttcgtgaata atgcaaaaag gcatgcagcg
    19561 tacctgggta gagtttgaac tcacagggct gctgatgcgc cgctaacgtc tggtaaagca
    19621 gacggctgtc atccagcagc ggatcgaact ccgccccggc aataaaacag ggcggaactt
    19681 cgcgagtgag atcattatta aacagacagt aatacggtga ctcgcggtcc gcgtcgttgc
    19741 ttaaatacgc ctcttcatac atctgcaaat cctgttgcgt taagccatcc cagacaccgc
    19801 ccaacagacg acgagtcacg gaatcccgta atccgtaaag cccataccac agcaaaacac
    19861 ccgcaatttt accgcaatcg atctgtttat cacgcaacca caacgcactg gcgagcgcca
    19921 gcatggcacc tgcggaatca ccggcaaagc caatgcggga catattgatt tgataatcct
    19981 ccgcctgctg gtggaaataa caacaagcag ccacaatttc ctctatcgct tgcggaaaac
    20041 gcgcttcagg tgaaaggggg taatcaatac caatcaccgt acattggctg tagcttgcca
    20101 gcaggcgcat gatgcgatcg tgggtatcga gattgccgag aataaaaccg cctccatgca
    20161 aataaaatag cgtcgccggg ctatctggct gcggacaaaa gagacgtgtt tccacctgcc
    20221 catattttgt tggaaccatg taagctctgg ttgccatttc tggagcgccc gcattccaga
    20281 atcggcgctc aagcgtgtaa tactgtcgtt gctcagcaat cgttcccgtt gcgggccagg
    20341 acggtaaatc cggctgaaga gtattcacaa cggtcttcat ttcagcagaa ataaggtcca
    20401 gaacaggtag tttgttttcc ggcttcatac aaaaactcct ttcaaattac gtcattgtaa
    20461 ggaaccactg ccatgaaaat gcgatcccgc ctgctgatat tgaaactggc tgcgtctcgc
    20521 gcgctcccgt cagattgtgt taacattcgc cgctcagtta accacccgta aaaacaacca
    20581 tgaaatttcc cggtaaacgt aaatccaaac attacttccc tgtaaatgca cgcgatccgc
    20641 tgcttcagca attccagcca gaaaacgaaa ccagcgccgc ctgggtcgta ggtattgacc
    20701 agacgctggt cgatattgaa gcgaaagtgg atgatgaatt cattgagcgt tatggattaa
    20761 gcgccgggca ttcactggtg attgaggatg acgtagccga agcgctttat caggagctaa
    20821 aacagaaaaa cctgattacc catcagtttg cgggtggcac cattggtaac accatgcaca
    20881 actactcggt gctcgcggac gaccgttcgg tgctgctggg cgtcatgtgc agcaatattg
    20941 aaattggcag ttatgcctat cgttacctgt gtaacacttc cagccgtacc gatcttaact
    21001 atctacaagg cgtggatggc ccgattggtc gttgctttac gctgattggc gagtccgggg
    21061 aacgtacctt tgctatcagt ccaggccaca tgaaccagct gcgggctgaa agcattccgg
    21121 aagatgtgat tgccggagcc tcggcactgg ttctcacctc ttatctggtg cgttgcaaac
    21181 cgggtgaacc catgccggaa gcaaccatga aagccattga gtacgcgaag aaatataacg
    21241 taccggtggt gctgacgctg ggcaccaagt ttgtcattgc cgagaatccg cagtggtggc
    21301 agcaattcct caaagatcac gtctctatcc ttgcgatgaa cgaagatgaa gccgaagcgt
    21361 tgaccggaga aagcgatccg ttgttggcat ctgacaaggc gctagactgg gtagatctgg
    21421 tgctgtgcac cgccgggcca atcggcttgt atatggcggg ctttaccgaa gacgaagcga
    21481 aacgtaaaac ccagcatccg ctgctgccgg gcgctatagc ggaattcaac cagtatgagt
    21541 ttagccgcgc catgcgccac aaggattgcc agaatccgct gcgtgtatat tcgcacattg
    21601 cgccgtacat gggcgggccg gaaaaaatca tgaacactaa tggagcgggg gatggcgcat
    21661 tggcagcgtt gctgcatgac attaccgcca acagctacca tcgtagcaac gtaccaaact
    21721 ccagcaaaca taaattcacc tggttaactt attcatcgtt agcgcaggtg tgtaaatatg
    21781 ctaaccgtgt gagctatcag gtactgaacc agcattcacc tcgtttaacg cgcggcttgc
    21841 cggagcgtga agacagcctg gaagagtctt actgggatcg ttaagttatc gtcggttcgt
    21901 aggccagata aggcgttcac gccgcatctg gcatttggct ctcgatgcct gatgcgacgc
    21961 tggcgcgtct tatcatgcct acatattttt catattttac atccggcaac caccgtttac
    22021 cccgtcacca cctcacccgc cggtggcgtt tccagcagtt ccagcatggt acgggcgatt
    22081 tcacgctcgc ccatcactac ctgattcgca ccacgttcgg tgatatacgc cacttcatcg
    22141 tcataatggg cgcgggcaat aatctcaata tccggatttt tcgcgcgggc agatgccaca
    22201 atctcacccg cttcataacc gttgggaatc gtcaggatca gccattttgc acattccaga
    22261 tgcgccagtt gcataatttc ttcgttcgcc gcattgccca atactgcgcg gaccccgcgc
    22321 tctcgcagct catcaacacg ggttcgtgac gtctcaatca ccaccagcgg aatatcagag
    22381 gcgagcaatt tctcccccag caggctgcct acacgaccgt aacccaccag tagcgcatgg
    22441 ttgcaaatat ccactgggat ctgcttctct tcttcgattg cctcttccag cgtctgctct
    22501 tccagcgttt cggtcttcgc cagatatttc tccagtagtg cgaacagtac cgggttgagc
    22561 ataatcgaca ggatcgcccc tgccagtacc aggttttgtc cggcctgcgg cagtaaattc
    22621 aatgccattc ccagtcccgc caggataaac gcgaactcac caatctgcgc caggctggcg
    22681 gcgatggtta atgccgtacg ttgggagtga ccaaacagtc gcaccaggaa aaatgcggct
    22741 aacgacttac caaacagaat aatcgccagc gtcgccagca ctgccagcgg ttgctgaatc
    22801 agaattaacg gatcaaacaa catcccgacg gagacaaaaa acagcaccgc aaacgcgtcg
    22861 cgcaatggca gcgtatcgtg ggcggcacgg tgactcagtt cagactcgtt cagtaccatc
    22921 ccggcaaaga acgcaccgag tgcaaaggag acatcaaaca gctctaccgc accaaaggca
    22981 atccctaacg ccagcgccag caccgacagg gtaaacagct cgcgagaacc ggttgccgcg
    23041 ctgcgtgcca taatccacgg caccagacgg cgacctacca gcatcataat ggcgataaat
    23101 gcgatcactt tgccgatggt gatccccata tcgactgcaa gagtggcaaa gcccacatcg
    23161 ccctgttcca tcattcctgc aactgcgggc agcaacacca gcgtcagaac cattaccagg
    23221 tcttccacaa tcaaccaacc gatggcgatt tgcccacgct gactgtcaat taattgccgt
    23281 tcttcaagtg cgcgcagtaa caccacggta ctggcggtgg aaagacataa accgaacacg
    23341 ataccggtca ttaacgacca gcccagcacg gcagagagcg ccatacccag cagcgtcgcc
    23401 acggctatct gggcgatcgc accgggaatg gcgatggcct ttaccgccat caaatccttc
    23461 agcgaaaagt gcaaaccgac gccaaacatc aacagaatga cgcccagttc agccagttcc
    23521 ggcgcaagct tggtatcggc aacaaagccc ggagtgaatg gtcctgccag cacacccgct
    23581 aacagatatc ccaccagagg agaaatacgt agtttattgg ccagcatgcc gaggataaag
    23641 gcgagcacaa ggccgccaac aatggtggtg ataagcgggg tggcgtgatg cattccgtct
    23701 ccttttcctg gtggttattg tccatttttg gccgggaaaa ccaaaattac aggtaatagt
    23761 ttatgacaat ttcattgatg atgttcatga ataattattg aattttgcag aaaaatggaa
    23821 ttagctgcaa aaaaagcacg gatcggaaaa cagaaggcgg tttaacagag gaaaggctta
    23881 tggcgactgt aaaacgatgc agccaaagtt tgcctttggc tgcaatgaaa tcagtcttta
    23941 tgccggttat caggcaggaa tatggtcaac atccccaata gtggcaggaa agcacagatt
    24001 ttatagacta actcgatgct ggtgtgatcg gcgataagcc ccagaactgc cgctcccaga
    24061 cctcccatgc caaaagcaaa accgaaaaag agtccagaaa ccataccgat acgtcccgga
    24121 agtagctcct gagcgtagac cagaatggca gagaatgccg aagcgaggat aaatccaata
    24181 atcaccgtta aaacccccgt ccagtgcagg ctggcgtagg gtaaaatcag cgtaaacggc
    24241 gcaacgccga ggatagagcc ccaaatcaca tatttccgtc caattttatc ccctacaggc
    24301 ccgccgatca ccgtacctgc cgcaacggca aacaggaagg caaacagatg aagctgggca
    24361 ttctggatag ataatccgaa tttttgcatc agataaaagg tgtaatag
//
LOCUS       DAJTYD010000045        24180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTYD010000045 DAJTYD010000000
VERSION     DAJTYD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.045"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..2104)
                     /locus_tag="OO585_001655"
     CDS             complement(<1..2104)
                     /locus_tag="OO585_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6629281.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKH"
     gene            complement(2215..3627)
                     /gene="ftsP"
                     /locus_tag="OO585_001656"
     CDS             complement(2215..3627)
                     /gene="ftsP"
                     /locus_tag="OO585_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6629282.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3702..4439)
                     /gene="plsC"
                     /locus_tag="OO585_001657"
                     /gene_synonym="parF"
     CDS             complement(3702..4439)
                     /gene="plsC"
                     /locus_tag="OO585_001657"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6629283.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4672..6930)
                     /gene="parC"
                     /locus_tag="OO585_001658"
     CDS             complement(4672..6930)
                     /gene="parC"
                     /locus_tag="OO585_001658"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6629284.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSLMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7476..7958)
                     /locus_tag="OO585_001659"
     CDS             complement(7476..7958)
                     /locus_tag="OO585_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6629285.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8011..8403)
                     /gene="ygiW"
                     /locus_tag="OO585_001660"
     CDS             complement(8011..8403)
                     /gene="ygiW"
                     /locus_tag="OO585_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6629286.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8555..9214
                     /gene="qseB"
                     /locus_tag="OO585_001661"
     CDS             8555..9214
                     /gene="qseB"
                     /locus_tag="OO585_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6629287.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9211..10560
                     /gene="qseC"
                     /locus_tag="OO585_001662"
     CDS             9211..10560
                     /gene="qseC"
                     /locus_tag="OO585_001662"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6629288.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10670..11251
                     /gene="mdaB"
                     /locus_tag="OO585_001663"
     CDS             10670..11251
                     /gene="mdaB"
                     /locus_tag="OO585_001663"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6629289.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11282..11596
                     /locus_tag="OO585_001664"
     CDS             11282..11596
                     /locus_tag="OO585_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6629290.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11644..13536)
                     /gene="parE"
                     /locus_tag="OO585_001665"
     CDS             complement(11644..13536)
                     /gene="parE"
                     /locus_tag="OO585_001665"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6629291.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13565..14146)
                     /gene="yqiA"
                     /locus_tag="OO585_001666"
     CDS             complement(13565..14146)
                     /gene="yqiA"
                     /locus_tag="OO585_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6629292.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14146..14973)
                     /gene="cpdA"
                     /locus_tag="OO585_001667"
     CDS             complement(14146..14973)
                     /gene="cpdA"
                     /locus_tag="OO585_001667"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6629293.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(14998..15420)
                     /locus_tag="OO585_001668"
     CDS             complement(14998..15420)
                     /locus_tag="OO585_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6629294.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15421..16050)
                     /gene="nudF"
                     /locus_tag="OO585_001669"
     CDS             complement(15421..16050)
                     /gene="nudF"
                     /locus_tag="OO585_001669"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6629295.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16255..17736
                     /gene="tolC"
                     /locus_tag="OO585_001670"
     CDS             16255..17736
                     /gene="tolC"
                     /locus_tag="OO585_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6629296.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            17884..18555
                     /locus_tag="OO585_001671"
     CDS             17884..18555
                     /locus_tag="OO585_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6629297.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18561..19721
                     /locus_tag="OO585_001672"
     CDS             18561..19721
                     /locus_tag="OO585_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6629298.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19759..20547)
                     /gene="ygiD"
                     /locus_tag="OO585_001673"
     CDS             complement(19759..20547)
                     /gene="ygiD"
                     /locus_tag="OO585_001673"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6629299.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20690..21451
                     /gene="zupT"
                     /locus_tag="OO585_001674"
     CDS             20690..21451
                     /gene="zupT"
                     /locus_tag="OO585_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6629300.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21509..21679)
                     /locus_tag="OO585_001675"
     CDS             complement(21509..21679)
                     /locus_tag="OO585_001675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629301.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(21939..22592)
                     /gene="ribB"
                     /locus_tag="OO585_001676"
     CDS             complement(21939..22592)
                     /gene="ribB"
                     /locus_tag="OO585_001676"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6629302.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22696..22844)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            22966..23256
                     /gene="ubiK"
                     /locus_tag="OO585_001677"
     CDS             22966..23256
                     /gene="ubiK"
                     /locus_tag="OO585_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6629303.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23540..24091
                     /locus_tag="OO585_001678"
     CDS             23540..24091
                     /locus_tag="OO585_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6629304.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
BASE COUNT         5990 a         6444 c         6042 g         5704 t
ORIGIN      
        1 tatgtttcgt caacgccgga cgggtattgc ggttctggcg acgagcttca cgcatctctt
       61 caatggttgg cgcaggcact aagcaatcgc gacggctacc aatcagatgc tttttaccca
      121 tcgcttccag cgcctggcgg attaacggcc agtttgccgg atcgtggtaa cgcaacaacg
      181 ctttatgcaa acgacgctgc ttgtcgccct tcggtacgaa gacgtcttca ctcttataac
      241 caatcttcgc cagcggtttt ttcccggtgt aatacatggt ggttgagttc gccagcggcg
      301 atgggtagaa gttctgtacc tggtcgagac ggaaacgatg ctttttcagc cacagcgcca
      361 gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga gatgaaatag gggatcagat
      421 actgctcttt acctgcctgt tttgagtaag tatcgaacag ctctttaaag cggtcatagc
      481 tgcccatgcc cggcttcatc atcttcgata acggcccttc ttcggtatgt tccggggcaa
      541 tcttcagata accgccgacg tgatgggtcg ccagttcttt gatatagcgc ggatcttcca
      601 cggcgatgtc ataacgcaca ccagaggcaa tcaggatctt tttaatgcct ttcagatcac
      661 gcgcacggcg atagaggttg atcgttggtt cgtggttcgt gtccatgtgc ggacaaatat
      721 ccggataaac gcacgacaaa cggcggcagg tttgctcagc gcgtggcgat ttgcagcgca
      781 acatatacat gttggcagtt ggcccaccga gatcggaaat tacgcccgta aaacctggaa
      841 cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc ttcggaacgg ctctgaataa
      901 tgcgcccttc gtgctcggtg atagaacaga aagagcagcc gccaaagcag ccacgcataa
      961 tgttgaccga aaaacggatc atttcgtaag ccggaatacg gacattgcca taggccggat
     1021 gtagcacgcg cttgtatggc agcgcaaaaa cgctgtccat ctcttcggta gaaagcggaa
     1081 tagcaggcgg gttgatccac acatagcggt cgccgtgttt ttgcatcaat gcgcgggcac
     1141 agcctgggtt ggtttcgtgg tgcagaatac gcgaagcatg ggcgtacagc actttatcgc
     1201 ccttcacttt ctcgaaagaa ggcagcaaca cgtaggtttt ttcccacggt ttcgggcgcg
     1261 gtggctgcac ggttacggct ttggcttcct gctttttcgg tgccaccggt ttgttatccg
     1321 cgcacggcaa atcttcacca tacggatgcg ggattgggtc gatttttcca ggggtatcaa
     1381 gacgggtgga atccacgccg ctccagccag gcagggcctc tttcacgata atcgcggtat
     1441 tacgcacatc gcggatttca ctaatcggct cgcccatcgc cagacggtgc gccacctcca
     1501 ccagcggacg ctcaccgtta ccaaacatca gcatgtcggc tttcgaatcc accagcacgg
     1561 aacggcgcac ggtatcggac cagtaatcat aatgtgcggt acgacgcaga ctagcctcaa
     1621 taccgccgag gatcaccggt acatctttcc acgcctcttt acaacgctgg gtataaacca
     1681 gtgtggcgcg atccgggcgc ttacccgcga cgttatccgg cgtgtaggca tcgtcatgac
     1741 gtaaacggcg atcggcggta taacggttga tcatcgaatc catgttgcca gcagtaacac
     1801 cgaaaaacag attcggttta cccagacgca taaagtcgtc tttgctgctc cagtccggct
     1861 gggcgatgat cccgacgcga aagccctgcg cttccagcat acgaccgcaa atcgccatcc
     1921 cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa aatgatgtcg cagctatccc
     1981 agccaagttg atccatctct tcactagaca tcggcaaaaa cggtgccggt ccaaaacagg
     2041 cggcccagta ctgcggccag gagaacaggt cgcgatccgg ttggatcagg gagatagagc
     2101 tcattttgct tccagaaatg ataaaaaaat aatcaaaggc cggggattat aagccggaac
     2161 gaaagagaaa tcgaaaagta ttccatactc gccctcctcg ggcgagtatg aagattacgg
     2221 taccggattg actaacagtt gcccaatcga gccacggtcc gccatttcca gcgtctgact
     2281 gttgaagtag aacgggaagt gcgcccagga aggctgaccg aaataaacaa gcagctccac
     2341 ttgtccgtca acccaaacgg tatctttcca gcctctgtct tccgggaacg gcatcgcacc
     2401 attcacgtta cggatctgga acatcacgcc ttcaatatgg aacgcttgcg gctcgtccgc
     2461 gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg acatcaatac ggttgacgtc
     2521 ccacagttgt ccattaatac ccgggtcatc acccagactg atatcgcgac tgcgaatcgg
     2581 cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga agactgtctg tgaccagcgg
     2641 cagaaggccg gttgggcgca gcgttagcac cagggtagaa accagaatgc tggatggctc
     2701 aaagaagcca cgaatacgat caacaatgct cgccgcttcg ccacaggtga tcgacacttc
     2761 atcaccgttg ctcatatcca ccagaatctc gcggcgctcg cccggtgcca acgaaagttg
     2821 cttcaccgat acaggagcag gcaggaatcc ctgatcgcca gaaatcacat gtaacgggcg
     2881 actatcgctc atctgtagtt gatagcgacg agagttcgac gcgttcagca gtcgcaagcg
     2941 cacccagcca cgcgagactt caacgtacgg gctttgtaca ccgttaacca gcagcgtatc
     3001 accaacaaag ccgccgcttc ccggttcgtt gtattctggc gtaccaaagt tatccagccg
     3061 tttatcctgg ataatgaccg gaaaatcatc cacaccataa tggttgggga taggcagcga
     3121 cttgctgact tcatcttcca ccagccacat tccggcaagg ccgttataga cctgctgagc
     3181 cgtgcggttg ggagtattgg cgtgatacca cagagtagct gcgttctggc gaatgggcag
     3241 tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc ggaccgccca tcagcgggcc
     3301 tggtacctgt agcccggcca ccgtcattga gacattttct gtcaggcggt tgctgtaaat
     3361 aagcttaaca tcgtcgccct tccagacgcg gatagtcggc cccaggtaac gaccattgat
     3421 tccccagacc gacgcgcgtg tccctggcgt aaatgaccag tgcgcacgtt gtacagtcat
     3481 aaacagcggt tgcccacggc gagattcaag tagcggcgga acgggtagcg gttgctgttg
     3541 cccggctgcg ctggccttca ggggaacagc gccggcacaa agtgcaatcc ccgatgcctg
     3601 aatgaactga cgccgactga gtgacatatt tgctccatgt aaaactggct aaagtaatac
     3661 cgtgcaggaa agtgttcccc gcaaaaatac agattgccga cttaaacttt tccggcggct
     3721 tcgcgttctg cgacttcttt atcgagctcg gcgatttttt gttccattat cgaacgacaa
     3781 tgggcagcca gctcacgaac ctgatctttg ccatactgac tgacgtcaat tggcggcagc
     3841 atttcgacaa tcaccagacc gttgtgcagt cgattaagat taatcttatt cgaagttgta
     3901 gagacgcaca cgggaataat cgggacgccc gccgcaattg ccgcgtgaaa tgctccagtc
     3961 ttgaacggta gcaggccgcg accacggctg cgggttcctt ccgggaacat ccagatggaa
     4021 atacggcgtt ttttgaaatg attcacgact tccgcaatgg tgccgtgagc tttagtgcga
     4081 ttgtttctgt cgatcaataa gttgccggtt aaccagtaca actgcccgaa gaaggggatc
     4141 cacagcaagc tctttttacc taccgtcacc gtcggcggtt gcacaatgtt cgatgctgtc
     4201 accatgtcat agttgttctg gtggttagcg atatagatag cattgccgta gctttcagca
     4261 tctgcaggtt tgcggcactc aactttcagg ccgaacagcg gcgcaagacg gccaaacata
     4321 tgcccaaagg tggccacatg tttcgggtta cgcgggctga aaaggcagta aatggagccg
     4381 aatacacaga ctaagatgct gtaaatcacg gtaataataa gacgaaagat atatagcata
     4441 acaacctctg aaggtctgag agactggcat tgtacgtcac ctgaaatcag gttagggcat
     4501 tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc aacgcatgaa taaacaacgg
     4561 tttcaaggga atttttatcg tttccggggg ttgaatacgc tgccggatga cgacttaacg
     4621 tttcatccgg cgttccttgc aagcgggagg aaacaggccc tccccggcat attactcttc
     4681 gctatcaccg ctgctggcac ggcgaggaga gtcgatctca acacgatcga tacgctgcaa
     4741 accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg actttctgta actcttccgg
     4801 gcgcagttta attttgcgtt tcccaacatg aatggtcagc gtgctttgcg gcggcagaac
     4861 gtacaattgc gccagaccat cttctccacg cgcggcttct gccgatggaa tgttgataat
     4921 cttgttgcct ttgcccttcg acagctgcgg cagatcgctt accgggaaca tcaacatacg
     4981 gcctgcctga gtgattgcca gcagcatatc ggaagcatct tcaatcacca ccggcggcat
     5041 aacatgggca ttttccggta aggtgatcaa agccttacct gcacggttac gcgccaccag
     5101 atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa gccatcagca gtttctgatc
     5161 gtcgctttcc atcagcatat ggtcaacggt cgccccaggc ggcaacgtta atttgccggt
     5221 gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc gggtcgatgg catagctacg
     5281 accggtggaa tcaacaaaca ctaccggttg gttgctctta cctttcaccg ccgctttgaa
     5341 gctatcaccc gctttataat tcaggcccgg cgcgtcgata tcatggcctt tagcgctgcg
     5401 tacccagccc atcagcgaca gcacaatggt gacaggttca gacggcagca tgtcgtgctc
     5461 gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa cgacgatcgt caccgtaggc
     5521 ttgcgcgtct gcctgcagtt ctttcttcag caggttattc attttacgct cggaaaccaa
     5581 aatgccctgc aactggtcgc gctctttttc cagctcactc tgctcaccgc gaatcttcat
     5641 ctcttccagt ttggcaagat gacgcagttt cagttcgagg atcgcttccg cctgggtttc
     5701 cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca tcttcgttac gaatgatctc
     5761 aatcacttcg tcgatgttga gaaacgccac cagcaaacct tccaggatat gcaggcgctt
     5821 gaggactttc tccagacgat agttcagtcg gcggcgcacg gtatcgcgac ggaacaccag
     5881 ccattcggag aggatttcca gcaggttttt caccgccgga cgaccatcca gaccgatcat
     5941 attgaggtta atgcgatagc tcttttccag atcggtggta gcgaagaggt ggttcatcac
     6001 ctgatccata tccacgcggt tggaacgcgg cacaatcacc agacgggtcg ggttctcgtg
     6061 gtcagattca tcgcgcagat cgtcaaccat cggcagcttt ttgttgcgca tttgcgcagc
     6121 aatttgctcc agtacgcgcg cacctgaaac ctgatgcggc aatgcgctga taaccaccgc
     6181 gccatcttct ttcttccaca ccgcgcgcat acgcactgaa ccacgtccgt tctcgtagat
     6241 tttacggatc tcggcgcgcg aagtgataat ttccgcttca gtcggataat ccggcccctg
     6301 cacgatatcc agcagctgat cgagcgtggt tttcggctgg tcgattaatg cgattgccgc
     6361 ctgagctact tcacgcaggt tatgcggtgg aatatcggtc gccatgccga cggcaatacc
     6421 ggtggtgccg ttaagcaaaa tgtttggcag acgggcaggc agcattttcg gctcctgcaa
     6481 agtgccgtcg aagtttggca cccagtcagc cgtcccctgc cccagctcgc tcaatagcag
     6541 ttcggaatat ttcgacaacc gggattcggt gtaacgcatt gccgcgaacg atttcggatc
     6601 gtccggcgcg ccccagttcc cctgaccatc aaccagcgga taacggtaag agaacggctg
     6661 cgccatcagg accatcgctt cataacaggc actatcgccg tgcggatggt atttacccag
     6721 tacgtcaccg acggtacggg ccgatttttt aaatttggcg ctggcattca ggcccagttc
     6781 agacatcgca tacacaatgc ggcgctgaac aggtttaaga ccatcgccaa taaacggcaa
     6841 cgcacggtcc atgatcacgt acatggagta gtttaagtag gcgttttccg taaattcatg
     6901 tagcgcaagg cgctctgcca tatcgctcat taattctgat tcctcaactt attcgccagc
     6961 cttcaaaggg gcaatattgc cggagatact acattatctg ccgcgtcgag tcacaggaga
     7021 tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat aaaggcatca atgtgccttg
     7081 atatacaact ctttgctgta gacttgatcg gcatagaggg cagcacaagg ttgcgcccat
     7141 gccacaccac tcggcatggg ggtccgctcc gggcgattcg agaagttgag gttatgcgag
     7201 tccagggaag cctaaagcat caaagtagtc gactgtaagt atacggttca gcccagttta
     7261 gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt gcggcttgct catggatacc
     7321 cccgagacgg cttacggcgc accggataat aaatttcgcg tatacatggt attccttacc
     7381 ggagaaatta taagcccaac aacaatacgc ctgtggcatt gttcgctcaa acttatcgcg
     7441 agtgatttgg ctcacactcc ggtaaagatt ccccatcacg ctaacggcac ataaacatcc
     7501 gtttccagtc gctgttctgt cataccttcg gcaagattgg tgtagtgaaa cagaatcggt
     7561 gctttacgca ttttctcgcc gcttgcaggc agccagtggc gaatgatgcc ccataccgtg
     7621 tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg cataacgtcc accggtaagt
     7681 tcaccattgc tcacaccata acgattatcg ggaatcggtt cgctaacgct gccgcagata
     7741 tcaaagcgaa acgctttcgg tgcggtggtg gcagggtcgt cccaggcgac gccaaaagtc
     7801 tggctttggt taacaggaga aagccccgtc tctttacgcc acatgataaa tttcgccaca
     7861 ctgtagttga gcaactcagg gctacagcgg tgcggcaaca tcgccacagg gattgatggg
     7921 aaatcgataa tgtttacatc cagtgtcagg tttgtcattt tgcctccctg cgaaattatt
     7981 cccgggaacg cgtcccggga gcggtaacaa ttacggattt actttgcgga tctgtttgac
     8041 gtcaatttca acagaattcc agtctttatc gacttccccc tgaatctcaa ccgtatcttt
     8101 cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata tcaacattga tagtaccgct
     8161 ggcatcttta aacacgtaga gatcgtcaga gatgcgttca acgatattgc cgcgcagggt
     8221 tacccaggtg tcgtcacgca gggattttgc gctttctaca gtcgttacgc tgccgttcgg
     8281 cccctggaat cctccggcct gactttgcgt tgccgatggg ccagaaaaac cgccctgctc
     8341 tgctgccatc accggtgcac tgcacagggc cattactgcg attactgctg cgaatttttt
     8401 catgtttatt actcccttta atgtctgttt ccgagcattt aacaagatag tccttaacaa
     8461 cttcttaagg gaaaaaaata aaatttaatg ctgtacagag cgcgttacaa cacggtttac
     8521 tggcagcaaa tacggttatc gcagggatga aaaaatgcga attttactga tagaagatga
     8581 catgctgatt ggcgacggca tcaaaactgg ccttagtaaa atgggtttta gtgtcgactg
     8641 gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc gcaccttatg atgcggtgat
     8701 cctggattta accttaccag gcatggatgg tcgcgatatt ttgcgcgaat ggcgagagaa
     8761 aggtcagcgt gagccggtac tgatcctgac cgcgcgtgat gcgttagcgg aacgtgtaga
     8821 agggctgcgt ctgggagctg acgattatct gtgtaaacct tttgcgttga tagaagtcgc
     8881 cgccaggctg gaggctctga tgcgccgaac caacggccag gccagcaacg agctgcgcca
     8941 cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg ctggctagcg aacccttaac
     9001 actgaaacca aaagaatttg ccctgctgga attactgatg cgtaacgctg gtcgggtact
     9061 gccgcgcaaa ctgattgaag agaaactgta tacctgggac gaagaggtca ccagtaatgc
     9121 cgttgaagtg catgtgcatc atctgcgacg caaactcggt agtgatttta ttcgtaccgt
     9181 gcatggtatt ggctacacat taggtgagaa atgaaattta cccaacgtct tagtctgcgc
     9241 gtcaggctga cgctaatctt tttaattctg gcctcggtga cctggctgct ttccagcttt
     9301 gtcgcctgga aacaaacaac ggataacgtc gatgaattgt tcgacaccca actgatgctg
     9361 tttgccaagc ggttaagtac gctcgatctc aacgaaatca acgcggcgga tcgcatggca
     9421 cagacgccaa ataaattaaa acacggtcat gttgatgacg atgcgctgac ctttgccatc
     9481 tttacccacg acggcagaat ggtccttaat gatggcgata acggagaaga tattccctat
     9541 agctatcaac gggaaggttt tgctgacggg caactggtcg gtgaagacga tccttggcgt
     9601 tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg ttgttggcca ggaatgggaa
     9661 taccgtgaag acatggcgct ggcgattgtt gccgggcaat tgatcccgtg gctggtcgca
     9721 ctgccgatta tgttaatcat catgatggta ctactgggtc gtgaactcgc gccgctgaac
     9781 aaactggcgc tggcactacg tatgcgtgac cctgactcgg aaaaaccact aaacgcgact
     9841 ggcgtaccca gcgaagtgcg accactggtt gagtcgctaa atcaactgtt cgcccgcaca
     9901 catgcgatga tggttcgaga acgacgcttt acctccgatg cagctcacga actacgtagc
     9961 ccgttaacag cgctgaaagt gcaaaccgaa gttgcgcagc tctctgacga tgatccgcag
    10021 gcacggaaaa aagcactgct ccaattacat tccgggatcg atcgcgctac tcgtctggtt
    10081 gatcaactgc tcacgctatc gcggctggac tcactggata accttcagga cgtcgcggag
    10141 atcccgcttg aagatctcct gcaatcgtcg gtgatggata tttaccacac ggcgcagcag
    10201 gcgaaaattg acgtgcgact gacactcaat gtccagggca tcaaacgcac cggacaaccg
    10261 ctattgctaa gtttgttggt gcgaaatttg ctggataacg ccgtgcgtta cagtccacag
    10321 ggcagcgtgg tggacgtcac gctgaatgcc gataatttca tcgtgaggga taacggcccc
    10381 ggtgtgacac cagaggcact ggcgcgaatt ggcgaacgct tctatcgccc ctccggacaa
    10441 accgctaccg gcagtggact tgggctatcg attgtccagc gaatcgccaa gctgcatggc
    10501 atgaatgttg agtttgggaa tgcggaacaa ggtggatttg aggcgaaagt aagctggtaa
    10561 gggtaagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc
    10621 gctctgtgac gtactatagt cggcaaacgt ctcaccttga ggttaaaaaa tgagcaacat
    10681 tctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga atgacaccct
    10741 gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgttcgca tcgttcgcgc
    10801 cgacagcgac tacgatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat
    10861 ctggcagatg ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat acattgatga
    10921 tgtattcacc gaaggtcacg ggacgctgta tgccagcgat ggccgtaccc gcaaagatcc
    10981 gtcaaaaaaa tacggttccg gcggcctggt acagggcaaa aaatatatgc tttctctgac
    11041 ctggaacgca ccaatggaag ccttcaccga aaaagatcaa ttcttccacg gcgttggcgt
    11101 cgacggtgtg tatctgccgt tccataaagc aaaccaattc cttggcatgg aaccgctgcc
    11161 gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata
    11221 tcgcaagcat cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac
    11281 catgcttacc gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt
    11341 attggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta
    11401 tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat
    11461 cgtgatgatt gagcagtggg aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca
    11521 catgaaggcg tacagcgaag ccgtaaaagg tgacgtgctg gagatgaata ttcgtattct
    11581 gcagccaggg atttaatcct gccttgtttg cccggccatc ctgaccgggc aatgttcttt
    11641 cctttaaacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc
    11701 cgagcgtttc ttcgccagta gcatgtccat catcgcgtca gtacgctgat cgtcttcatc
    11761 atcgatagtc aactgcacca gacggcgagt gttcggatca agcgtggttt cgcgcaattg
    11821 catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg
    11881 ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt
    11941 cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa
    12001 cgcgcggaag tgttttacaa acaaagcgca gagcagcgtg gcaatgtgca gaccatcgga
    12061 gtcagcatcc gccaggatac agatcttgcc gtaacgaagc tggctcaaat cgtcgctgtc
    12121 aggatcgata ccgatcgcta ccgaaatatc gtgcacttcc tgcgaagcca gcacttcgtc
    12181 ggaagagact tcccaggtgt taaggatctt acctttcagt ggcatgatcg cctgatattc
    12241 gcgatcgcgc gcctgcttgg cagatccgcc tgcggagtca ccttccacaa gaaacagttc
    12301 ggtgcggtta aggtcctgcg cggtacagtc agccagtttg ccaggcagcg ccggaccgct
    12361 ggtcagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat
    12421 cgccatctct gccagcaact ccgccgcctg aacgttctgg ttcagccaca ggataaaggc
    12481 atctttcacc acgccagaaa cgaatgccgc gcactgacgc gaagagagac gctctttggt
    12541 ctgcccggca aactgcggat cctgcatttt tactgacagc acataggcgc agcgatccca
    12601 gatatcttcc gccgacagct ttacaccgcg cggcagaatg ttgcggtatt cacagaactc
    12661 acgcatcgcg tccagcaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt
    12721 tgggatcagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag
    12781 cgcccagtcc accgcttcag tatcgccagc gaaattaccg ataaacggtt tttctggcag
    12841 cgtcggtaaa ccgtttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca
    12901 gcgctgttcg gtgttgttga tctcatcttt aaaggtaatc tcaacgccgg ggcacagtac
    12961 tgctttggct ttcagcacat gcgtcaggcg tgaaacagaa aaacgcgggc tatcgaagaa
    13021 ggtttcatcc ggccagaaat ggacgctggt tccggtattg cgtttaccgc aagtgccgac
    13081 aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa tctgaccatc
    13141 gcggcgcacg ttaacttcta cgcgcttcga cagggcgtta accaccgaaa tccccacgcc
    13201 atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcctg catgcagacg
    13261 gcaaagaatc agttcaaccg ccggtacacc ctcttccggg tgaatatcca ccggcatccc
    13321 gcgcccatcg tcaataactt ctaacgactg gtcagcatgt aaaataacgt ccacgcgttt
    13381 tgcgtgaccc gccagtgctt catccacact gttatcaatg acttcttgcc ccaaatggtt
    13441 agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag
    13501 tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagta attcgagttg
    13561 atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc
    13621 ccgtgaatgc gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca
    13681 ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg
    13741 gcgcttccag cgggtcaatc tgcatgactt taagatcgta aatatggcgt gactctagca
    13801 catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcagttcaa
    13861 acgggcgcac cgccgggttt accaccactg cgggcagcat aaaacattgt gacaaccagg
    13921 tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca
    13981 ggacaatgga ttccagcagc tctgccgcgt cggaaggata cggcggcaac tgcggaatga
    14041 tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag
    14101 agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga
    14161 agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc
    14221 atcagcatgt aactcgagag tacgccagcc gggcgcgatg gtatccagcg taaagttgga
    14281 acagtgcggc ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca
    14341 gtcgagatcc agctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt
    14401 cgccagcacg gtatccagtt cgcccgcgtt acgcagactg tgttgatcga gccaactaca
    14461 acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc
    14521 cagtttacgt tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa
    14581 cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacacgc gcttcgccgg
    14641 ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgcccgg
    14701 cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc
    14761 agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctgatg
    14821 tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc
    14881 ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatcctga ctctggcctc
    14941 accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta
    15001 gtaaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa
    15061 ctggttaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg
    15121 cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata
    15181 cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat
    15241 tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc
    15301 cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg
    15361 tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat
    15421 ttatgcccac tcatttttta acgcttggtg gtgcagctgc agccattgca aagcgatgac
    15481 cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac
    15541 cacatgaacg cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt
    15601 ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag
    15661 gaaacttaac accggtttgg tccgtttgac tatcagcccc gcctcttcaa tcgcttcgcg
    15721 acgggcaaca tcttccacac tttcaccctc ttcaatcatc ccggcaacca tctccagtag
    15781 ccagggggtt tcgctagtgt cgtacgcggc aattcgaatc tgctcaatca gcacaacttc
    15841 atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttcccg
    15901 ccgcacctca tgactcattt gcccgttgaa tagactatga cgaaatctat aaagatctaa
    15961 tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa
    16021 tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta
    16081 aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac
    16141 attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg
    16201 caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag
    16261 aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc
    16321 gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct
    16381 gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca
    16441 cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc
    16501 aactcgaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa
    16561 tggcgtgcgt taacgctgca ggaaaaagca gcagggattc aggacgtcac atatcagacc
    16621 gatcagcaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt
    16681 gacgttcttt cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc
    16741 acccaacgtt ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag
    16801 tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag
    16861 cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac
    16921 tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac
    16981 ctgtcgctgt tacaggcacg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg
    17041 caggatggtc acttaccgac gctggattta acggcttcta ccgggatttc tgacacctct
    17101 tatagcggtt cgaaaactcg tggtgccgct ggtacccagt atgacgacag caatatgggc
    17161 cagaacaaag tgggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg
    17221 caggtgaaac aggcccagta caactttgtt ggtgccagcg agcaactgga aagcgcgcat
    17281 cgtagcatcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcatc tatcagtagc
    17341 attaacgcct acaaacaagc cgtagtttcc gctcaaagct cattagacgc gatggaagcg
    17401 ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcaaccac cacgctgtac
    17461 aacgctaagc aagagctggc aaatgcgcgt tataactacc tgattaatca gctgaatatt
    17521 aagtcagccc tgggtacgtt gaacgagcag gatctgctgg cactgaacaa tgcgctgagc
    17581 aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt
    17641 gctgatggtt atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact
    17701 accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc
    17761 gtctgaacgt aagacaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc
    17821 ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc ccggaagaca
    17881 aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc
    17941 catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctag ctgtgaaaag
    18001 agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc
    18061 aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg
    18121 ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgccagcag
    18181 gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggcccagca atccagcggg
    18241 agtttctgga tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg
    18301 ggatttgcac agcagccgct gttctcctcg aaaaacccag ccagcccggc ttacggtaaa
    18361 tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta
    18421 ccgaagacag ccatggcacc aaaaccggcg accactacta ccgttacccg tggcggtttt
    18481 ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caaccggcac ctcttctcgt
    18541 tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg
    18601 aaaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg
    18661 aagatgctta ctacaagttg cccctcgccc aggttgaaaa gctggaagaa gtcaccgccg
    18721 aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga
    18781 ccaaattccg cattccaaaa cacacctgga gttttgtgcg tcagtcatgg ctgactcacc
    18841 agccatcgct ttattcgcgt cttgatctgg catgggatgg cactggcgaa cctaaactgc
    18901 tggaaaataa cgccgataca ccaacgtcac tatacgaggc ggcgttcttt cagtggatct
    18961 ggctggaaga tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc
    19021 tgcaagaaaa gctaatcgat cgctttgtcg agttgcgtga acagtatggc ttccagttgc
    19081 tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg
    19141 actgcgcgac ggaagctgaa atcgctaccg agttcctcta cattgacgat atcgggttag
    19201 gtgaaaaagg tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt
    19261 atccatggga atttatgttg cgtgagatgt tctccaccaa gctggaggat gcaggcgtac
    19321 gctggctgga accggcgtgg aagagcatta tctccaacaa agcgcttcta ccgctactgt
    19381 gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc
    19441 cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga
    19501 tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga
    19561 ttgttcagca attccatccg ttaccgaaat tcggcgacag ctatatgctg attggtagct
    19621 ggctggtgaa cgatcaaccc gccggaattg gcattcgtga agaccgtgca ttgatcaccc
    19681 aggatatgtc tcggttttat ccacatattt ttgttgagta atcaatgatg ccggatggca
    19741 ttcgccatcc ggtaattgtt aacctatctg caccgacagc atactcaggc tgcccatttc
    19801 tataccatcg accggaatgg taattggctc ctgcccatcc cacgcaccta agacatacaa
    19861 caacggcaga tagtgctccg gcgttgggtt cgataacgcg ccaccttcat ggtcgaggta
    19921 attcaccaga ggatgctgct ctaccgcccc ttgccacgtc agattcgctt tcacatactc
    19981 attaaacgac atcgcccaag gatatggtga actatcaccg tgccacttca ctgtacgcag
    20041 gttatgcacc acgttgccac tggcgaccaa cattattcct tcatctcgca gcgctgccag
    20101 tttgcgcccc atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac
    20161 catcgggata tcggcgtcag gatacatctt aatcagcacg ccccaggaac cgtggtcaaa
    20221 gcccctggct tctttatcca gcgtcaccgg gaccggcgct aacagctcaa ccagatgctg
    20281 tgccagcaca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc
    20341 accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa
    20401 ccagtgagcc gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg
    20461 ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa
    20521 caatgctggc atacgtgttg aagacatgat gatatcctta actaagggtg tcattttgat
    20581 atcctcacaa tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc
    20641 agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc
    20701 tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct
    20761 cggacaaaaa ccctcgaacc gcttactggc gttttcgcta ggttttgcgg cggggatcat
    20821 gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatatcgcc
    20881 tgtgttgggt tatggaatgt ttatcttcgg tctgcttggc tactttggcc tggaccgcat
    20941 gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc
    21001 gatcaagcgc acagccattc tgctcactct cggcatcagt ctgcataact tcccggaagg
    21061 gattgccacc tttgtcacgg cgagcagcaa cctggagctg ggatttggca tcgcactggc
    21121 cgtcgcgttg cacaatatcc ctgaaggtct ggcagtggca ggcccggttt atgcggcaac
    21181 ggggtctaaa cgcaccgcga ttctgtgggc ggggatttct ggactagcag aaattcttgg
    21241 tggtgtgctg gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcggt
    21301 tgcaggaatt atggtggcgc tctcggttga tgaattaatg ccgctcgcca aagagattga
    21361 ccctaataat aaccccagct atggcgtact atgtggaatg tcagtgatgg gattcagttt
    21421 agtgctgcta caaacggcgg gaattggtta aaaacaacat cagaagttgt ccagcacatg
    21481 gacaacttct gccagcttca cgaaagcctt attctacctt ttcggctgtc tcttctctcg
    21541 tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg cgaaacgccc
    21601 gacaataacg tttcaaatgc aggaaataac ccaccacaac cagagcaatc aatacaatcc
    21661 agtaccaggc gataaacatc ctttaccctt aatatttaac aatactaaac atttagcgca
    21721 taaatttcac atatcctttt tcggatatat tcatatggtc gcataataac ggaacaatta
    21781 tatgattttg atcataatga aaaagaatgc ctgatattaa tgaaattttc tcatagaaag
    21841 agaagcggaa ttaacatctt gcatagcagg gcaaaataaa aaccgaagcc agctacaact
    21901 tcggtttctt gattaaggca gtaattaagc agcggttttc agctggcttt acgctcatgt
    21961 gcctgacggt atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca
    22021 aactcaatgc actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagc
    22081 acaccagccg gtttaaagcc tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg
    22141 ccacgcgtca gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc
    22201 agatcagaag gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg
    22261 gcagaaacac cggtagtcac accttcagcc gcttcaatgg tcacggtaaa accggtgcca
    22321 taggcgctgg tgttattttc taccatcatt ggcagatcaa gttgtttacg gcgatcttca
    22381 gtgatgcaca ggcaaacaat accgctaccg tggcgaatgg tcagcgccat ctgctcaacg
    22441 gtcatggttt ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc atcatcaagc
    22501 accattacac cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc
    22561 gtaccaaaag aggaaagtag cgtctgattc atggtaaaaa aaactcacta aaattatggt
    22621 taccagaatc agggcagtct taggagtggc ggcatatagc caaaataacg tgagcgggtc
    22681 catgcccgac agaatcgtta ctctctccca tccggactct aaccgtcggc cccggaatta
    22741 caccggatct gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt caactgagtt
    22801 gatttaccgc cggtggggaa tttcgccccg ccctgagaat aagcggattc actataacgc
    22861 taatgattag cggcagcaac gcatagcttc acataattct ggtttatgac ttacccttat
    22921 cgcactacaa tggtactcaa cacctatcag tacagggaaa ctacaatgat tgacccgaaa
    22981 aaaattgagc aaatcgctcg ccaagttcac gaatcaatgc ctaaaggaat cagggagttc
    23041 ggggaagatg tggagaaaaa aatccgccaa accctacaag ctcagctgac gcgcctcgac
    23101 cttgtaagcc gtgaagagtt cgacgtccaa acgcaagtcc tgttacggac tcgtgagaaa
    23161 ctggcgctgc ttgaacaaag aatgagcgaa ctggaaaatc gcagtactga gattaaaaag
    23221 cagccagatc cagagactct ccccccaacg ctgtaaacat ttgtctttaa tgcactgtaa
    23281 attatgaatt aatcttctaa tttacagtgc tttctattac cttatcgctt aattccattc
    23341 agaatatcac cacaaaaatg aatgagtgca attgaaaata taattgttat gtattttttt
    23401 aagttttact taatccatat ttagataaac taatttcata aaacccgaaa agtaaaatat
    23461 aatatttgaa tgattagcaa tccttaaata acagttacta atcattcatt ttctacttat
    23521 atttatataa aggaatatta tgtctgcttt taaaaagtca cttcttgttg ctggcgtagc
    23581 aatgattctt tccaataacg tatttgctga cgaaggtcac ggcattgtta aatttaaagg
    23641 tgaagttatc tctgctccat gttcaattaa accaggtgac gaagacttaa ctgtaaatct
    23701 gggtgaagta gcagataccg ttctgaaaag cgaccagaaa tctttggctg agcctttcac
    23761 tattcacctg caagactgca tgttgagcca gggtggaacc acttattcaa aagcgaaggt
    23821 tactttcacc acagcgaata ctatgacggg tcagactgac ctgctgaaaa acaccaaaga
    23881 aactgaaatc ggtggtgcca caggcgttgg cgtacgtatt ctgaatagcc agagcggcga
    23941 agtgacttta ggtactccgg tagtaattac attcaacaat actaacagct atcaggaact
    24001 gaacttcaaa gttcgtatgg aatccccggg caaagatgct accccgggca acgtttacgc
    24061 tcaggctgac tataagattg cttacgagta atcacattag gccgggttcg cccggccaca
    24121 tcattaacgg attaatgata agtggaacag atgtataaaa aattaaaatt aaccacaata
//
LOCUS       DAJTYD010000046        24101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTYD010000046 DAJTYD010000000
VERSION     DAJTYD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.046"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..2623)
                     /gene="valS"
                     /locus_tag="OO585_001679"
     CDS             complement(<1..2623)
                     /gene="valS"
                     /locus_tag="OO585_001679"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6629305.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVSL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAE"
     gene            complement(2623..3066)
                     /gene="holC"
                     /locus_tag="OO585_001680"
     CDS             complement(2623..3066)
                     /gene="holC"
                     /locus_tag="OO585_001680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6629306.1"
                     /translation="MKNATFYLLDNDTTVDGLSTVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3226..4737)
                     /gene="pepA"
                     /locus_tag="OO585_001681"
     CDS             complement(3226..4737)
                     /gene="pepA"
                     /locus_tag="OO585_001681"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6629307.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5004..6104
                     /gene="lptF"
                     /locus_tag="OO585_001682"
     CDS             5004..6104
                     /gene="lptF"
                     /locus_tag="OO585_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6629308.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6104..7186
                     /gene="lptG"
                     /locus_tag="OO585_001683"
     CDS             6104..7186
                     /gene="lptG"
                     /locus_tag="OO585_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6629309.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7347..8849)
                     /locus_tag="OO585_001684"
     CDS             complement(7347..8849)
                     /locus_tag="OO585_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6629310.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(8979..9998)
                     /gene="ahr"
                     /locus_tag="OO585_001685"
     CDS             complement(8979..9998)
                     /gene="ahr"
                     /locus_tag="OO585_001685"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6629311.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            10194..10278
                     /locus_tag="OO585_001686"
     tRNA            10194..10278
                     /locus_tag="OO585_001686"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10228..10230,aa:Leu,seq:caa)
     gene            10442..11707
                     /locus_tag="OO585_001687"
     CDS             10442..11707
                     /locus_tag="OO585_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6629312.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(11762..12772)
                     /locus_tag="OO585_001688"
     CDS             complement(11762..12772)
                     /locus_tag="OO585_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629313.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(12723..13259)
                     /locus_tag="OO585_001689"
     CDS             complement(12723..13259)
                     /locus_tag="OO585_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629314.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            complement(13260..14339)
                     /locus_tag="OO585_001690"
     CDS             complement(13260..14339)
                     /locus_tag="OO585_001690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629315.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            complement(14737..15309)
                     /locus_tag="OO585_001691"
     CDS             complement(14737..15309)
                     /locus_tag="OO585_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HCR6629316.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(15383..15883)
                     /locus_tag="OO585_001692"
     CDS             complement(15383..15883)
                     /locus_tag="OO585_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR6629317.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(15880..16614)
                     /locus_tag="OO585_001693"
     CDS             complement(15880..16614)
                     /locus_tag="OO585_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HCR6629318.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            17166..17432
                     /locus_tag="OO585_001694"
     CDS             17166..17432
                     /locus_tag="OO585_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6629319.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            17429..18028
                     /locus_tag="OO585_001695"
     CDS             17429..18028
                     /locus_tag="OO585_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6629320.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            18021..18308
                     /locus_tag="OO585_001696"
     CDS             18021..18308
                     /locus_tag="OO585_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HCR6629321.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            18301..18756
                     /locus_tag="OO585_001697"
     CDS             18301..18756
                     /locus_tag="OO585_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629322.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYQDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            18861..19214
                     /locus_tag="OO585_001698"
     CDS             18861..19214
                     /locus_tag="OO585_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HCR6629323.1"
                     /translation="MQSSVTGRKANENTLLPPVLRAALYRRAVACAWLTVCERQHRYP
                     HLTLESLEAAIAAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVV
                     MDELCARHIKAPVLH"
     gene            19229..21562
                     /locus_tag="OO585_001699"
     CDS             19229..21562
                     /locus_tag="OO585_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6629324.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            22140..23408
                     /locus_tag="OO585_001700"
     CDS             22140..23408
                     /locus_tag="OO585_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6629325.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            23799..>24101
                     /locus_tag="OO585_001701"
     CDS             23799..>24101
                     /locus_tag="OO585_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629326.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQS"
BASE COUNT         5718 a         6251 c         6009 g         6123 t
ORIGIN      
        1 gctctgcgcc gtcgatgatc ttcgtaacgg aaaccggacc tttgtcatcg gcaggcagca
       61 cggtgatgct ttccagacgc gccagggttt gcaggaagcc acggttttca tttacgcgac
      121 gttctgcatc cgcgctgcaa ccacgcagca gcagctccag cggtttgccc ggcgcgatgt
      181 tcatttctgc acggatgtta cgtaccgcaa cgatcgcctg tttcagccac tcggtgtcag
      241 ccagtgcggc ttcatcaacc tgagatgcat cgtactgcgg gaacggctgc agcatgatgg
      301 tgtcggcagt gataccgcaa agtactttca cacgctgcca gatggtttcg gtgatgaacg
      361 gaatgatcgg atgcgcgagg cgcagcagac cttccagtac agtcaccagc gtatggcgag
      421 taccgcgcag ttcggcttcg gttccaccgt tcattaccgg cttagtcagc tccagatacc
      481 agtcgcagaa ctggttccag gtgaactcat acagaatgcc tgcagcgata tcgaagcgga
      541 agctgtccag cgcttcgcgg tacgctttga tggtctggtt gaactctgcc agaatccagc
      601 ggtccgccag cgacagcgtc atttcaccac cgttgaagcc gcaatcctga ccttccgtgt
      661 tcatcagcac aaagcggctg gcgttccaca gcttgttaca gaagttacgg tagccttcca
      721 ggcgtttcat atcccagttg atgtcacgac cggtagaagc cagcgccgcc agggtgaagc
      781 gcagggcatc ggtgccgtgc ggctcaatgc cgttcgggaa ctgtttctcg gtgcgcttac
      841 ggattttgtc cgccagctgc ggctgcatca tattgccggt acgtttctcc agcagttccg
      901 gcagcgaaat accgtcaacc atatccagcg ggtcaataac gttacccttg gatttggaca
      961 tcttctggcc ttcgtcgtca cgaatcaggc cggtcatgta aacggtgtgg aacggcacct
     1021 gcggtttgcc attttcatct ttgatgaagt gcatggtcat catgatcatg cgggcaatcc
     1081 agaagaaaat gatgtcgaaa ccagatacca tcacgctggt tgggtggaac tggcgcagtg
     1141 cgtcggtgtt ttccggccag cctagggtag agaaggtcca cagcgcagaa gagaaccagg
     1201 tgtcgagaac gtcttcgtcc tgacgcaggg caacgtcggc accgaggtta ttttccttgc
     1261 gcacttcttc ttcgttgcgg ccaacataaa cgttacctgc ttcgtcgtac catgcaggga
     1321 tacggtgacc ccaccacagc tgacgagaga tacaccagtc ctgaatatcg cgcatccagg
     1381 agaagtacat gttttcgtac tgcttcggta cgaactgaat gtcgccgttc tcaaccgcct
     1441 caaccgccgg tttcgccagt acatcggcac gcacgtacca ctggtcggtg agcatcggtt
     1501 cgataactac gccgccacgg tcgccgtaag gcacggtcag gtcgtgcggt ttaatttctt
     1561 ccagcaggcc gagcgcgtca accgcagcaa cgactgcttt acgtgcagca aaacgctcca
     1621 gtttctggaa ctctgccggg atttcgctgg aataaacgtc agattcgtta cctttggtat
     1681 cgaacacctg ggcgctttca cggatatcgc cgtcaaaggt caggatgttg atcatcggca
     1741 gcgcgtgacg tttacccact tcatagtcgt taaagtcgtg cgccggagtg attttcacgc
     1801 agccggtgcc tttttccatg tcggcgtgtt cgtcgccaac gatcggaata cgacggttaa
     1861 ccagcggtag aatgacatat ttgccaatca ggtctttgta acgcggatct tccgggttaa
     1921 cggccacgcc ggtatcgccc aacagggttt ccggacgggt agtcgcaacc accagataat
     1981 ctttaccgtc tgcggttttc gcgccgtcag ccagcggata gcggatgtgc cacatcgaac
     2041 ctttcgattc gcggttttcc acttccaggt cagagatagc ggtgcgcagt ttcggatccc
     2101 agtttaccag gcgtttgcca cggtaaatca ggtcttcttt atacagacta acgaaaactt
     2161 ctttcaccgc attggacagg ccttcgtcca tcgtgaagcg ttcacgctcc cagtcgacgg
     2221 agttgccgag acggcgcatc tgacgggtaa tcgtgccgcc ggattccgct ttccattccc
     2281 aaattttgtc gatgaaagct tcgcggccgt agtcgtgacg ggttttacct tcttctgcgg
     2341 caatcttgcg ctcaacgacc atctgggtag cgatcccggc gtggtcagta ccgacctgcc
     2401 acagggtgtt tttgccctgc atgcgctgat agcggatcat ggtatccatg atggtttgct
     2461 ggaaggcgtg acccatatgc aaactgccgg tgacgttcgg cggcgggatc atgatgcaga
     2521 aactttcctg gctttcatcg ccattaggct taaagtagcc ctgcttttcc cagtgctcgt
     2581 aaagcggctg ttcgatatct tgtgggttat atgtcttttc cattatttcc aggttgccgt
     2641 attcaggttg aaaccagcca cgcggtaggc tttatagcgt tcgcgcgcca gttgtttcag
     2701 agaatcttca taaggaacga agtctaccac ttctgtgaaa gcggtggcaa aatctgcaaa
     2761 gcttgttcgc agactaatca atatatcgcg cgggctgctg ctgcgttttt gcggccaggc
     2821 gatctccacc ggcgcaccgc cgcgcggtcc ttctcccgct aaattatgcg gaacaaagct
     2881 ttctgccgga cgcgcccaca gggcttcatc cagccggtaa gcctgctttt catcttcaca
     2941 ggcgatgagc acgcgcttac cgctgcgcca acgttctgcg gcaatttcac acaccagttg
     3001 ctcaacggtg cttaagccat cgacggtggt gtcattgtcc agaaggtaga acgtcgcgtt
     3061 tttcatatat ggggcttctt gtggtggatt taaatacgca tcaggtatac accgcagcgc
     3121 ctgatgcgac gctgttgtgt cttatcaggc ctacgagttc agtgctgtgt aggtcggata
     3181 aggcgttcac gccgcatccg gcaataacag ccttgcctga cgcaattact cttcgccgtt
     3241 aaacccagcg cggtttaaca ggaactgtgc cagcaacgcc accggacgac cggtggcgcc
     3301 ttttgcttta ccagaacgcc aggcggttcc ggcgatatcc aggtgcgccc agttgtactt
     3361 acgggtaaag cgcgacagga agcaacctgc ggtaatcgcc ccaccaggac gaccgccaat
     3421 gttcgccata tcggcaaaat tggactccag ctgttcctga tactcgtcac ccagcggtaa
     3481 gcgccatgcg cggtcaccgg attgttcaga cgcggcaatc agttcatggg ccagcggatt
     3541 atggttcgcc atcagaccgg taatgtgatg acccagcgcg atcacgcagg caccggtcag
     3601 cgtcgccacg tcaatcaccg cttccggctc aaaacgctca acgtaagtta acacgtcgca
     3661 cagtaccagg cggccttcag cgtcggtatt cagcacttca acagtttgac cggacatggt
     3721 ggttaacaca tcgcccggac gataggctcg tccgccaggc atgttttcgc agcctgccaa
     3781 cacgccgata acgttaatcg gcagttgtag ctccgcgacc atccgcatca cgccgtaaac
     3841 cgctgccgca ccgcacatat cgtacttcat ctcatccatg ccttctgaag gcttgatcga
     3901 gataccgccg gagtcgaagg ttaaaccttt acccaccagc acgattgggc gtgcatcttc
     3961 cgacgcgttg cctttgtact caatcaccga catcagcgat tcgttttgcg aaccctgacc
     4021 gaccgccaga taggaatgca tccccagctc tttcatctgc tgttcgccga taacgcgggt
     4081 gatgacattc ttgctgtagc tgtcagccag ctggcgcgct tgtgaagcga ggtaagcggc
     4141 gttacagata ttcggcggca tattgccgag atcttttgct gctttaatcc cggcggcaat
     4201 cgccagaccg tgcaggatcg cgcgctcacc gctggtcagt tcacggcggg tcggcacgtt
     4261 gaacaccatt ttacgcagcg gacgacgcgg ttcgctcttg ttcgttttca gctgatcgaa
     4321 actgtagagc gtctcttttg ccgtctcgac agcctgacgc actttccagt agttgttacg
     4381 gcctttaacg tgcagttcag tcagaaagca gaccgcttcc attgagccag tatcattcag
     4441 cgtattaatg gttttctgaa taacctgctt gtactgacgc tcatccagct cacgttcttt
     4501 gccgcaacca ataaggagaa ttcgctcgga aagtacattc ggaacatggt gcagcaacaa
     4561 tgtctgcccc ggttttcctt ccagttcgcc ccgacgtagc agggcgctga tgtacccatc
     4621 gctgatttta tcgagctgtt ctgcaatcgg agaaaggcga cgtggttcga agacgcccac
     4681 gacgatgcag gcactccgct gtttctccgg gctaccgctt tttacactaa actccatgca
     4741 ctacgctcct gaatcttaaa gacaacggcg gtggctacag atagaattgc aagctttcgt
     4801 aactcatgtc cgctgttgcg atgacttcgt gttaatctta acgttattac ggcattggca
     4861 cgtcagaaca aaatctgaga ggtgaatccg ttgagtataa ttatcttagc gacgatttcg
     4921 acgactcaag agaataaatg acgtttaagc catgaaacaa gctaaaatcc tgcaaaagac
     4981 gagtttttac gggcgtattt aaagtgataa tcataagata tctggtgcgg gagacgctca
     5041 aaagccagct ggcgatactc ttcatcttgc ttttgatctt cttctgtcaa aagttagtga
     5101 ggatcctcgg cgcagcggtt gacggcgata ttccggcgaa tctggtgctc tcccttctcg
     5161 ggttgggcgt gccggaaatg gcgcagctta tcctgccatt aagcctgttc ctcgggctgc
     5221 tgatgacgct gggcaaactg tataccgaaa gtgaaattac ggtaatgcat gcctgcggcc
     5281 tgagcaaagc ggttctggtg aaagcggcaa tgatccttgc ggtattcact gcaatcgtcg
     5341 cggcggttaa cgtgatgtgg gcgggaccgt ggtcatcgcg tcatcaggat gaagtgttag
     5401 cagaagcgaa agcgaaccct ggcatggcgg cgctggcgca agggcaattc cagcaagcga
     5461 ctaatggcag ctcggtgctg ttcatcgaaa gcgttgacgg cagcgatttc aaagatgtgt
     5521 tcctcgcgca aattcgacca aaaggtaatg cacgtccttc tgtggtggtg gccgattccg
     5581 gacatttaac ccagctgcgc gacggctccc aggtcgtcac tctcaaccag ggaacgcgct
     5641 tcgaaggcac tgcactgtta cgtgatttcc gcattacgga cttccaggat tatcaggcga
     5701 tcattggtca ccaggcggta gcgctcgacc cgaacgatac cgaccagatg gacatgcgta
     5761 cattgtggaa cactgacacc gatcgtgccc gcgcagaact gaactggcgt atcacgttgg
     5821 tattcaccgt gtttatgatg gcacttatgg tcgtaccgct gagcgtggtt aacccgcgtc
     5881 agggacgcgt actatcgatg ctgccagcca tgctgctgta tctacttttc ttcctgatcc
     5941 agacctccct gaaatcgaac ggcggtaaag gtaagctgga cccgacgctg tggatgtgga
     6001 ccgttaacct gatttatctg gctttggcga ttgttctcaa cctttgggac accgtgccgg
     6061 tccgccgcct gcgcgccagt ttttcgcgta aaggagcggt gtgatgcaac cttttggcgt
     6121 acttgaccgc tatatcggta aaactatttt caccaccatc atgatgacgc tgttcatgct
     6181 ggtgtcgctg tcgggcatta tcaaatttgt cgatcagctg aaaaaagccg ggcaggggag
     6241 ttacgacgcg ttaggcgcag gaatgtatac cttgctgagc gtgccgaaag atgtgcaaac
     6301 attcttcccg atggcggctc tgcttggggc gttgcttggt cttgggatgc tggcgcagcg
     6361 cagcgaactg gtggtgatgc aggcttctgg tttcacccgt atgcaggttg cgctgtcggt
     6421 gatgaaaacc gccattccgc tggtcttgct gacgatggcg attggtgaat gggtcgcgcc
     6481 gcagggcgag cagatggcgc gtaactaccg tgcgcaggcg atgtacggcg gctcgttgct
     6541 ctctacccag caaggcttat gggcgaaaga tggcaacaac ttcgtttaca ttgagcgggt
     6601 taaaggtgac gaagagttag gtggcatcag tatttatgcc tttaacgaga atcgtcgtct
     6661 gcaatccgta cgctatgccg ctactgcgaa gtttgacccg gaacataaag tctggcgtct
     6721 gtcgcaggtt gatgaatctg atctgaccaa tccgaaacag atcaccggtt cgcagacggt
     6781 gagcggcacc tggaaaacca acctcacgcc ggacaaactg ggcgtggtgg cgctggaccc
     6841 ggatgcactc tcaatcagcg gtttgcacaa ctacgtgaag tatctgaagt cgagcggtca
     6901 ggatgccgga cgttatcagc tcaatatgtg gagcaaaatc ttccagccgc tatccgtggc
     6961 ggtgatgatg ctgatggcgc tgtcgttcat ctttggccca ctgcgtagcg taccgatggg
     7021 cgtgcgtgtg gtcaccggta tcagcttcgg ttttgtcttc tacgtactgg accagatctt
     7081 cggcccgctg acgttggttt atggcatccc gccgatcatc ggcgcactgt tgccaagcgc
     7141 cagcttcttc ttaatcagcc tgtggctgtt aatgagaaaa tcgtaaccga atgaacgaat
     7201 aaagggagcg aaagctccct ttattattgt tagcaaagtg tgcttcgttc atgcctgaaa
     7261 aatcatgaaa atccaatata ttgcagtggt gtaggcatga taagacgcgc cagcgtcgca
     7321 tcaggcaaag acaaatctcg cctgacttac cttcttcttc cccccagcaa actccccaac
     7381 atcccacgta caatctgatt cgtcacctgg cgggcggcgc ttttcgccat tgtttgcacc
     7441 acaccatctt tcttcccgcc gcgtggtccg gtagtgccaa acaaaatatc cttcaatcca
     7501 ccaagaatac cgtcatccac cgccaccgct ttccctttcg cgggaggatt attttgctgc
     7561 ttggtactgg cctgaaagcc tttttgcaac atctcatagg cggattcccg gtccacgtca
     7621 tcctcgtact tgccatacac cggggagtgg ttaatcaagc cattgcgctc atcttccgtc
     7681 accggtccca tccgcgaaca aggcgcaatc accatcgccc gttccaccac agaaggactt
     7741 ccttttgcat cgagaaacga gattaacgcc tcgccggtcc ccagttcctg gattgccttt
     7801 tcggtatcaa acgccggatt ggcccgcatg gtttgcgccg cagcttttac cgctttctga
     7861 tctttgggcg taaaagcccg caaagcgtgt tgaacgcgat taccgagctg cccgagcaca
     7921 ttatccggaa tatcagacgg gttttgcgaa acgaaccaga cgcccacgcc ttttgagcga
     7981 ataagcctta ttacctgctc aatcttatcc agcagtacct gcggtgcatc gttaaacagc
     8041 agatgtgcct cgtcgaagaa aaacaccagt tttggtttct ccagatcgcc cgcttccggc
     8101 aattgttcat acaactctga aagcatccac agcaggctgg cggcgtacag tttcggcatc
     8161 tgataaagct tctcggcgct gaggatattg ataacgcctt taccgttggt atcggtgcgc
     8221 atccagtctt tgatatccag catcggctcg ccaaagaaat gcgccgcacc ttgcttttcc
     8281 agcgacagta aaccgcgctg gatggcacca accgatgcgc tactgatatt accgtactga
     8341 ttctggaaag atttggcgtt atcgccgatg tactgggtaa tcgcccgcag atctttaaag
     8401 tcgagcagca acaatccctg atcgtcagca atacggaaga tgatattcag cacgccagat
     8461 tgcacatcgt tgagattcaa cagccgcgcc agcaacagcg gccccaggtc tgaaaccgtc
     8521 gcccgcaccg gatggccttt ctcgccaaag atatcccaca ccaccaccgg attagcatgc
     8581 ggttgccagt cattgacgcc gatattttta agccttgtga gcagtttttc cgacgccgtt
     8641 cctgcctgcg cgataccggt cagatcgcct ttcacatcag ccataaacac cggcacgccg
     8701 atttccgaca atgactctgc cagtttttgc agcgtaacgg ttttacccgt ccccgttgcg
     8761 ccagtaatca gcccgtgacg gttagccatt cccggcagta aaaaaagttc tgtgtccggc
     8821 gtgcgggcaa ttaatagggg ttcgctcatg agatttcctc cagtttcctg cctggagtat
     8881 aggcaacggc tgcgcactct tcaccgctaa attcagcaga ttgcgagacg ccttccaaag
     8941 agatttaagt gccgggcgac aggcacccgg caaagggatc agaagtcggc tttcagaacc
     9001 acgcggtaac gcgccttacc gtcgcgcaca tgcttgatgg catcgttaat tttcgacatc
     9061 gggaacagtt cggtggtcgg cgcaaccttg ctacgggcgg caaagcgcat cagctttcgc
     9121 agctcataag gcgtgccggt agcagaacca gagatgctgc ggtcgcccgc aattaatgta
     9181 aaggccggaa cagacagcgg cgtgagaacc gcaccgaccg tatggaaatt accgccatag
     9241 gtcagcgcct caaaataagg ctgccagtcg aggctgacgt tcacagtatt gataatgaga
     9301 tcaaactgcc ccgacagggc tttcagtgcc tgcggatcgc ggctattcac cactttatcg
     9361 gcacccatcg ccagtacttc ctgctctttc gccggattag aactaaaggc cgtcacctcg
     9421 catcccattg cgtgcagaag ttttatagcg atatgcccca gcccgccaat accaattacc
     9481 ccaacgcggc tggtagcagt gatatggtgc atcaacagtg gtttaaagac cgtgataccg
     9541 ccgcacaaca gcggcccggc ggactcgata tcaatatttt caggcagtgg aatcacccat
     9601 tgccagtccg cacgcaactt ctcggcaaag ccaccgcgat tcataatcgt cggcaccgca
     9661 ccttgctcgc agttgatctg attaccgcta atacaggtgt cgcagtgacc acagctacgc
     9721 gccgtccagc caatcccgac acgctgaccg acctgcaaac ctttatcctg cgcggcgctc
     9781 ccgagtgcca ccacgcgccc aatcacctca tgcccggcaa ccagcggata ttgtgaaaat
     9841 ccccattcat tgtcgatcat cgatagatcg gaatggcaga tcccgcaata atccacctgc
     9901 acttcaacat cttgtggcct cagctcaccg ggatcgtact cataaacttc cagttcgccg
     9961 cccgcttctt ttgcggcata gctttttatc atcgacattt tttggtcctt ctctggtgtt
    10021 gtttgggaag tgtagagcat ggcagggcgg ggtgcctgga gtgtgacaaa ggttacacat
    10081 cgctgtgtgc aatgctgaaa atttcagcac ttagcgaggt gcgagcaagc tggcgcttgc
    10141 atggtggcgt gcgacaggta taatccacaa cgttttccgc atacctcttc agtgccgaag
    10201 tggcgaaatc ggtagacgca gttgattcaa aatcaaccgt agaaatacgt gccggttcga
    10261 gtccggcctt cggcaccatt cttacttcca agaccatccg agaaagtcca aatatccctt
    10321 taaaatcaat gcttgcagcg attttttcgt cctgcgtctt ccgaggttgt ccgttgaaat
    10381 ccggatatca ttgggggcat aattgggggc atcttaactt cgattagaaa tgtgccccca
    10441 aatgaagctc aacgccagac aggtcgagac cgcaaagcca aaagacaaaa cctacaaaat
    10501 ggccgatggt ggtggtttgt atcttgaggt ttcggccaaa ggttccaaat actggcgcat
    10561 gaaatacaga cgtccctctg acaaaaaaga ggatcgtctc gcgtttggtg tttggcctac
    10621 tgtgacgctt gctcaggcaa gagcaaagcg cgatcaagct aaaaagctgt tagcgcaggg
    10681 cattgatcca aaagccgaac agaaagaagc tcaggccgag aattcggggg catattcttt
    10741 cgaaactatc gctcgtgaat ggcatgctag taataagcgc tggagtgaag atcaccgatc
    10801 acgcgttcta cgttatcttg agctttatat ctttcctcat attggttcat ctgacatccg
    10861 tcagctcaaa actagccacc tattagcccc gattaaaaaa gttgatgcca gtggtaagca
    10921 tgacgttgca cagcgccttc agcagcgtgt cacggctatt atgcgttatg cagtacagaa
    10981 cgattacatc gactcaaatc cagccagtga tatggccggt gcgctatcga caaccaaagc
    11041 gcgacattac cccgctttgc cctccagccg gttccctgag tttcttgctc gtcttgctgc
    11101 atatcgtggc cgtgtaatga cgcggatcgc ggttgagctt tccttactaa cttttgtgcg
    11161 ttccagtgag ttacgtttcg cgcgttggga tgagttcgac ttcgataaat ctctctggcg
    11221 agtacctgca aaacgagaag aaattaaagg tgtgcgttac tcgtaccgtg gcatgaagat
    11281 gaaagaggag catatcgttc agcttagtcg gcaggcgatg attttgctaa accagttaaa
    11341 gcagattagt ggagataaag agctgctttt tccgggtgat catgacgcaa ctaaggttat
    11401 gagcgaaaac acggtaaata gcgcattgcg tgcgatgggc tatgacacga aaaccgaagt
    11461 ttgcgggcac gggtttagaa cgatggcccg tggtgcgttg ggcgagtccg gattatggag
    11521 tgaagatgct atagaacgac agttgagcca ttcagagcgt aataatgtac gtgctgctta
    11581 tattcacacc tctgaacatt tggatgaacg tcgattgatg gttcaatggt gggctgatta
    11641 tctccaagca aataagggca aaacaataac accttatgat tttgctaaaa taaacaaaag
    11701 tcgatagtga aaataaacgc ctataatata taggcgttta tttctgtatt aaacactttg
    11761 atcataatct tccagcagca actttttttc gtacttcatc tttccaattg ccactatgga
    11821 ttattctatc gaaattactt cctgctcttc ctgataataa gtcaattcga gatagcttgc
    11881 tgatatcatc ccagttatca tagaggatat atatagcatt accatattct atgttttcaa
    11941 atacaaccaa atcgtcctcc agcatagctc caatataatt actgaacgtg ctgtttcctg
    12001 tgacgatttt tttaggttca agactcatga agaagtcata tctattttca gcgacatttt
    12061 tcttgtcgga agtgatattt ttacctctaa aaattcttgc taaagtttct tctttactac
    12121 ctggtggaaa tatttcccat gagactataa gtgtatcagc ataagtcgat atgggtttat
    12181 cagcatcctc aattccacat ttaccaagat tttcttgaag aagatttaaa tcaaataata
    12241 attcatcatc aaacttattt gattgtttgt ctaaaattct agttactctg aagttaaata
    12301 tgaaatggct gtccgagggc tgtttgtgat taatttcaat ggctatgagt gttggtggta
    12361 taatgtctct gggatatttc tcgtaaggta atctaacagt atgtgtacca ttagagctat
    12421 caccccagtt aggtgactca atttcagtgt aatgagtttc ttttgggagg tcttttctga
    12481 caatttcttc accaaaaaca tttttaagtg aatatttacc ctgcaatgga tctggtatga
    12541 ccgacgtctc ataagcatta acaccattat caaaatatat tccaaggtgc tctaatgctc
    12601 cactttttaa ttggttttcc gtaaatgcaa tagaagtgcc agcaacaact gtattaccaa
    12661 gccttcttaa cttgctatga atatttttag gtataacacg tatattcttt ttcatttttc
    12721 cctcatactt gatcatcaat gtctctttgc tctcgcttgt tatgtaaatc attgataaac
    12781 ataacatttg caagtattac gctaattact gcctcaatct gcatgtacga aatagcaata
    12841 attaatgcta aagatgtgtt ttttattaca tcttggctct ctaaataaag atgacttttg
    12901 aaaaacacta gtttaactaa gtatacaaca agtaaagtaa tcataacgaa agatgatttt
    12961 agaacacttc caactattgc ttctaatctt cttgtatctt ccagttgttc ggtgaaatca
    13021 acggtttcta tagttttggg gttcacaata cgctgcaaag catttgggta aagaaacgct
    13081 aaccaaattc ccataatagt aaaaaccatt ccagaaactg atacgagaag agtgcaatag
    13141 ttggcaaaat cgttatacac atagttaata tcggatgacg aaataatcat cagcgatata
    13201 atgaatatcg ccatgtatat ccatgtgtat ttggctatgc ctgctatagc tttcaatatt
    13261 caatcctctc cagatgcttt ctttattctt ggttgagatt gtaatgcttt taattctttt
    13321 aatatagggg aaataaattc ttttcttttg tgtgatattt gttcataaat atatgcggcg
    13381 gaatatgttg aatagtcgtc agaagggaca ttgagaatat cacgcattct atatttatcc
    13441 acccaagtta tacccttgtc tgtagcaaat ccaacattgt cccaaagacg tttttctcta
    13501 tcctcagccg aatacttttc aatgatttct tgaacttcat tcaaggatgg ttttgcttct
    13561 gcttttattt caatttgtct tgttttggca tttttctttc cggacacaaa tggaacgaga
    13621 gtgctaaagg tctttaacca atcagctcgt gcatcatttg gatttataat aatagtttcc
    13681 ctgcgaacta tgtgtgttat ttttggtatt agtttaccta actcagtatg agtggtttct
    13741 aagcttctga gcttcatatc aaacctgtac atatatttat ataagtcttc gtcatcggta
    13801 ttagatagcc ttatataacc tttctcggtt ttatttttaa cccttttgct atgtttaact
    13861 ctattagtaa tagatgagtg tacatagtct tgaaatagtt cagaatcaca aacagaatgg
    13921 tcgaatttaa tggatactat agtttcaagt tcaggtataa cccaataaaa gcatggccgc
    13981 ccccagataa cttttttccc tctgtagcta ttagtatatt taaccaccga tgagcttccg
    14041 atttcaccgt cttcactagc tcctaataaa gagcccgtac tatcagtatc agatttccat
    14101 aacatgacta agaaatcacc agtattttca tccttataaa tatccttgca ataacacttt
    14161 gaactgtttg gtttttcttt tggatcccat ggtatcgtga gtgcaagaga tttagttccc
    14221 acccaatcct gtatgaggtc aaatgtttcg ctaagttcta aaccataggt attgtcatca
    14281 tttaccttgt ataaaccgca cttattaacc ttgaagaaat tgagatgccc tacattcatg
    14341 ctctttcctt agcttcttat cttgggatat gtatcatgat caaaaattaa gcataacccc
    14401 attgacttcg tgcgggcaat gttttcaccc tagaatttga tcggtttaac gttaaatatt
    14461 cataagttac atgagcattt ttcacttctt cccaattaca gacgtcatga gctgaatatt
    14521 aataaaataa ttatcctcac ggcatggcgc gcaatgctct ccccgccacg cccgcccgct
    14581 ttgcagggcg gttttaatgc agttgcacag tcacgctcag gctgcgccgg gaatggcgcg
    14641 gtctgcagaa aatgaggcag gaaaacgcat gcaaagccat gcaccttatc gatgcatggc
    14701 ttttttcagt aaaaacgggc ggattttcgg ggaattttac acagactgac gtgatgccag
    14761 ttgcgcactt ttacgcgaaa aaatcatgtt ctgcgcaggg gtgaattttt cacggctgtc
    14821 atccaccgaa gtcgcgtcag gcctgaatcc gatggtcgtt aaaatgtcac tatcctgtgc
    14881 ggaataatta attttttccc ccttcgcaag ccaggacaga agggcttcac gcagggcatc
    14941 tgtggcacgc tgtatggcac agtttcgggc aatggccgtc agctcactgt agcccatcag
    15001 ctccggtgcc agtgccgccg ccagtgctgt gccgtgctgc tgcataaaat cattcagccg
    15061 gtcgcggatg ctgatgtgct gaacggcttc atgcgaacga atataacgac cggcagcctg
    15121 attcacctgc cattttctga cttcgataat attgcgtaat tcgtccaggc gactgacgtt
    15181 tctgccttcc ccggacagaa gccgcagata ttcctgttcg gccgcagcca gctcattttt
    15241 gcgttgcagc catgctgctt tgttattctg acaggtgtca aaggcctgct gtaaggctgt
    15301 gctttccatc gttatctctt tctcatcatg ctgaagaata aaaatacggt gtgcggcgac
    15361 ggccggtgtt aaccggcagc cctcattcca gacgcagcga atatgattgt gtttttaagc
    15421 gtactggcgg cagttcctgt ttttcatgca ggctttctgc cagttcgtcc ggcgtgaccg
    15481 ggcggacaat aaaacggttg atggtctgaa gcgttttaaa caccagacca cagcccggat
    15541 ccgtgcacac ataaaaacgc tcggtgactt cctgagacag acgccgcgat gttcttgaca
    15601 gtgcaaggcc tttacatctc cgacaacaat atccggtaac aagcattctt ttcgggcgtt
    15661 tcatactgcc ggaggctgac gtcagtgaat cgcggtatct ctgtttgcct gaaatgtatt
    15721 ccattcctga atctttacag tcagagaaaa agctttcact cgcttcaaat gtcgcagagc
    15781 aataaatatt ccggcactgt gcaatcatta tcttggtgcc atcgtccatg aaatgtgcgc
    15841 gacgggtgtg agcaacatgt ccacacgacg ggcagtaaat catgacagca gtcctctggc
    15901 cttaagctct gctccctgct ggtctatttt gtcctgccac accttgcgct gtgccggtgt
    15961 gcctgccacc tcataatcca tgtgcgggag tgttgccgct gacagtccgg tcagccggag
    16021 aaccggctcg ccggtgaggc tgatttgcat ctgtttaatt ttctgttcca gcgatgattt
    16081 cacctgctgc atgacagcct tttccggtgc gacgtagccc tgatggccgg tggtgttggc
    16141 gagcggattt tcctgtacca ggatactcag atgcattgcc cggacaagcg cctcacaggt
    16201 ttcattcagg gcgtgttcca gctcatgctc tgcatacaga ctcagcaggt gatgatgtgc
    16261 cttccggtag gcggtggccg tgctgtcaca cgcccctttc aggcgttcac gttcaaaatt
    16321 cagcaccacg gccagattgt catattcctg taccagctcc cggcgtgcca cgcgctcaat
    16381 gtggcgctgt ttcagctcgt cgctcaggac accaccggct gcacgaaagg ccgtgcgcca
    16441 gtcgtcagcg tcatttccgt cggcctgcgc cagcgcattt ttttcctgct ctgcccgttc
    16501 aatggccgtg acggtctcat ccatcaggcg ggcgttctca aggtgggcgg ctctggcctt
    16561 ttccagttgt gccagtgcgg gttgcagata ttcagggatg gtgttgtcag acattttccg
    16621 gctcctcgtc acttcaggtt aagaaaattg tgacgtacac cggacaacaa cacgacgcat
    16681 tgcagatgtg ccagccctga cacaggagac tcatcctcag accggcaagc caggaaaagg
    16741 tcgcaggaaa aaccggctta ctgtttgttt ttttatattt tactgttcac ctctgttcac
    16801 cataagaaaa aagataagta atacagtaag ttaaagggtg aacaatcgca gtaatgactg
    16861 ttcaccgtct gttcaccact gttcacccgc tcatagactt tttgtgctgt ttattacttt
    16921 ttatttttat taatccactg aaataaataa gaaaaaacaa tttgtatttc actataattt
    16981 tttccagctc cttccagagc gttttaaagc catctggcgc tgatgtataa aaaatacaca
    17041 gccattgtaa gactgccgga acaaatcccc cctgttgcat ctgccaaaaa tattcacaaa
    17101 ataaagcgct acccgaagcc ggacggactt atccggtgct gtatggacat taacgaggta
    17161 gcccgatgca agctgttttt tcttccccgt ctcccgcccc tgtgacgcca ctgatgccgc
    17221 tgccggacat cacgcaggag cgttttttac gtctgccgga agtgatgcac ctgtgcggcc
    17281 tgtcacgctc gaccatctat gaactcatcc gtaaggggga atttccgccg caggtgagtc
    17341 ttggcggtaa aaatgtggcc tggctgcact ctgaagtcac cgcatggatg gccggacgca
    17401 ttgccggacg caaacggggg tacgacgcat gatgatgccc gctctgcaaa aactcccttt
    17461 ttctggcttg cctttttccg gcatttgcgg atatagtttt tccgctgccg caaaatcggc
    17521 agccgggcgt gagaacccga gcaattcaca ggcgacatat gacgcgccac gcgtcttttt
    17581 ttgtgtcgca atcaatgcca cagagcgcca gattatggtg tggtgtgtgg tcagtcgtgc
    17641 agatggcatt ccgtgcatcc tgcccgcgtc cgctcattat gccgcagagt caatggtagc
    17701 tcaggcgggg cagcctccgg gctggccggt atcctgtgaa gccggtattc tcacccccgt
    17761 ctgggctatc gccatcgagc gtgagaactc cggcgatagc gttatttgct attcacagga
    17821 ggctgccatc atggctacaa cccttacccc ctcacaccct gaatttgtct ttgtgtttgc
    17881 ggctgtccgt cgcgcagacc gtcatccccg tatctgtatg cttcgcaccg tggccggtga
    17941 tgaacgcagt gcccgccgtt cccttgtccg tgactatgtg ctctcccttg ctgcccgtct
    18001 gccggtggtg gaggtgtccc gtgcgtaata aaaaagcccc tcagaccgtc tcagcacgtc
    18061 atgacgcccg tgaacacctc agcattgagg cttaccataa gctcaaccgc gccagcgccg
    18121 tatcccagtt tgttgggggt gatttgattc accgtgaact ctccggcctg catcagctct
    18181 acattccgca tattttcagc tacctgaatg aagatattga ttttgtgctg aatgagctga
    18241 aagccaaagg cctgtgccgc gattttctcg cccagcagaa agaccgggga gacaggacgc
    18301 atgtttgatt ttccccagcc cggtgagatt taccgttctg ccggttttcc cgatgtggcc
    18361 gtggtcggca ttctggaaga cggtattccg tgggaaatgc cgtaccgctg cccggacatt
    18421 gtctggaacc cgtaccgccg taaattcagt atccttgtgc gtatcctcgc cgacgggcgc
    18481 accacagaca tcccgctggg gcgttttctg cgggaattta cctgtgaccg tcctgacctg
    18541 tttaaacgca gccccgtaaa ccggcatgcg gtactgaaag aaatggccgg agacccggaa
    18601 ttacagaaat ggcgggagaa atatcaggat atttacccgc aggacactgt tccggtcagc
    18661 cgggcagcac cggtagcgcg ggaatggcgg gaaattcccc gcacggagcc tgacccggaa
    18721 atcaccccgg ataacagtta ccgcaattat ctgtaattaa aaaacgacac ccgaaaaatt
    18781 aaatgtgcgt attcgcgcag ggatacgcac gtcttcagga gacgcagata tgccttatca
    18841 gttaatgcaa ccggcacgga atgcagtcat ctgtcacagg gaggaaagca aatgaaaaca
    18901 ccctactacc gcccgtctta cgcgcagccc tttaccgtcg tgctgtcgcc tgtgcctggc
    18961 tgaccgtgtg cgaacgtcag caccgctacc cgcatctcac ccttgagtca ctggaggcgg
    19021 ccatcgccgc tgagctggaa ggcttttatc tgcgccagca cggtgaggaa aaagggcgtc
    19081 agatagcctg tgccctgctg gaagatttaa tggaatccgg ccccctgaag gccgcaccgt
    19141 cgctgtcctt tctcgggctg gttgtgatgg atgaactctg tgcccgtcac ataaaagcgc
    19201 cggtactgca ctgaaggaga acaacaccat gaaaatgaac gtaaccgcca ccgtcagcca
    19261 tgcgctcggc cactggccgc gtattctccc ggcgctgggg attcaggtgc tgaagaacca
    19321 tcatcagccc tgtccggtct gtggcgggag tgaccgcttc cgttttgatg acagggaggg
    19381 gcgcggcacc tggtactgca atcagtgtgg tgccggtgac ggcctgaaac tggttgaaaa
    19441 ggtgtttggt gtctccccgt ccgacgtggc cgcaaaggtg gctgccgtga ccggcagtct
    19501 gcccccggct gacccggcag tgacggccgc cgccgttgct gaaacagacg ctgcccggaa
    19561 gaacgccgcc gcactggcac aaaccctgat ggcaaaaacc cgtcccggaa ccggtaacgc
    19621 ctacctgacc cgcaagggct ttcccggccg ggaatgccgg atgctgaccg gcacacacag
    19681 agccggtggc gtgagctggc gcgccggtga ccttgtggtg ccactgtatg acgacagcgg
    19741 cgaactggtt aaccttcagt taatcagtgc tgacggccgt aagcgcaccc tgaaaggcgg
    19801 acaggtcagg ggcacctgtc acacccttga aggacagaat caggccggaa aacgtctgtg
    19861 gatagcggag ggatacgcga ccgcacttac cgtacatcac ctgaccggtg aaacggtgat
    19921 ggtggcgctt tcttccgtga acctcctttc tctggccagc cttgcccggc agaagcatcc
    19981 ggcctgtcag attgtccttg ccgcagaccg tgacctcagc ggtgacggcc agaaaaaagc
    20041 cgccgcagcc gcagatgcgt gtgaaggtgt tgttgccctg ccgccggtct tcggtgactg
    20101 gaatgatgcc ttcacgcagt acggcgggga ggtcacccgt aaggccattt acgatgccat
    20161 ccggccaccg gctgaaagcc cgttcgacac catgagcgaa gcagagtttt ccgccatgag
    20221 taccagcgaa aaggccatgc gtatctatga gcattacggc gaggcgctcg cggtcgatgc
    20281 caacggccag cttctgtccc gctatgaaaa tggtgtctgg aaggtgctgc cgccactgga
    20341 ctttgcccgg gatgtggccg ggctgtttca gcgtctgcgc gcgccgttct cctccgggaa
    20401 ggtggcctcc gtggtggaca ccctgaagct gattattccg cagcaggaag ccccctcccg
    20461 ccgcctgatt ggctttcgta acggtgtgct cgacacacag aacggcacgt tccacccgca
    20521 cagtccgtca cactggatgc gtaccctgtg cgatgtggat ttcaccccac cggtggaagg
    20581 ggaaacgctg gaaacccacg cccccgcgtt ctggcgctgg cttgaccgtg ccgccggtgg
    20641 ccgtgcggaa aaacgcgacg tgattctggc cgcactgttt atggtgctgg caaaccgcta
    20701 cgactggcag ctctttctgg aggtgaccgg tcccggcggc agcggcaaaa gtatcatggc
    20761 cgaaatcgcc accctgctgg ccggggagga taacgccacg tcggccacca ttgagacgct
    20821 ggaatccccg cgtgaacgtg ccgcgttaac tggcttctca ctgatacgcc tgccggacca
    20881 ggaaaaatgg agcggcgacg gtgccggact caaggccatc accggcggcg atgcggtgtc
    20941 cgtggacccg aaataccggg atgcgtactc cacgcatatc ccggcggtga ttctggccgt
    21001 gaacaataac ccgatgcgct tcaccgaccg cagcggcggc gtgtcacgcc ggcgggtgat
    21061 tattcacttc ccggaacaga tagccccgca ggagcgcgac ccgcagctta aggacaaaat
    21121 cacccgcgag ctggcggtca tcgtgcgtca cctgatgcag aagttcagcg acccgatgct
    21181 cgcccggtca ctgcttcagt cccagcagaa ctcagacgag gcgctgaaca tcaaacggga
    21241 tgccgacccg acgtttgatt ttatcggcta tctggaaacc ctgccgcaga ccagcggcat
    21301 gtatatgggg aacgccagta tcatcccgcg taattaccgt aaatacctct atcacgccta
    21361 tctggcctac atggaggcaa acggctaccg gaatgtactc agtctgaaaa tgttcgggct
    21421 ggggctgccg gtgatgctga aggaatacgg actgaattac gagaagcgcc ataccaaaca
    21481 ggggatacag accaacctga cactgaaaga ggaaagctac ggcgactggc tgccaaaatg
    21541 tgacgaccct gcaacaacct gacccacctg accggcatct gccggtcttt ttttatcccg
    21601 acctcccccg aaggtgaaca atccactgtt cacccttcac cgtatattca cccgttatca
    21661 cactgaaatt aaaagagaaa aatgaaaggt gaacagtgtg aacagttctt tcgaaaaaaa
    21721 attttttatg tggataaaag atgcactggt ttggatccgg gctacggatc caaacttact
    21781 gattaaaaat tatttaactt tccgatttat tgatgggctg aaaatagatt ggtcagaaaa
    21841 acagtggggg cacaaaaggg ggcatatttc attgttgtgt tttattattt gttttttatc
    21901 attaacttat tttgatattt gagtccggcc ttcgcaccaa aagtatgtaa atagacctca
    21961 actgaggtct ttttttatgc ctgaaatccg cgctacacaa ggcttttcca gcttttcccc
    22021 tcaactgaag tcaacctgac tcaacccaca tcaacaggct tgtgagtata ctaatgagta
    22081 tatttgccga ttcgatattg cttgtatact cacgagatac caatggaagg aagaccatta
    22141 tggccctaac ggatactaaa gttcgctctg cgaaacctga ggagaaagag tattcacttg
    22201 ttgacggcga cggcatgtcc ttacttgtaa aacctggcgg ttcaaagtat tggcgatttc
    22261 gtttccgttt tggcggtaaa caacatttga tggcgttcgg cgtttacccg gatgtttcgc
    22321 tggcggatgc gcggaagaaa agggaagagg ccagaaagct ggtagctgct ggtatcgatc
    22381 ctcgtgagca taagcgtgct gtgaaagaag agcaggcgaa agagattatt actttcgaga
    22441 aggttgccag agagtggctc gtaaccaacc aaaaatggtc ggaagagcat gctaatcgtg
    22501 taaaaaagag cctggaggac aatatcttcc cggcaattgg ttcccacaat attgctgaac
    22561 tgggcacccg cgatctgtta attcctatta aagccgtgga gaaatccgga cgtcttgaag
    22621 tggcttcccg ccttcaacag cgcaccacgg ccatcatgcg ttatgcagtg caaagcgggt
    22681 taattgatta caatccggcg caagagatgg cgggggcggt agcttccagt aatcgacagc
    22741 atcgtccggc gctggagtta aagcgtatcc cggaattact ccagaaaatc gatgaataca
    22801 ccggcaggcc actaacccgc tgggcaaccg aacttaccct gcttattttt attcgatcca
    22861 gtgagctgcg ttttgctcgc tggtcagaaa tcgattttga aacgtcaatg tggaccatcc
    22921 cgcctgagcg agaacctatt cccggtgtga aacattctca gcgaggagca aagatgcgta
    22981 cacctcatct ggtgccgctt tcaaagcagg cgctagcgat cctgaagcag ataaaacagt
    23041 tctgtggcga acatgagctg atttttattg gcgatcacga tccgagtaag cccatgagtg
    23101 aaaacacggt gaacagtgca ttgcgggtga tgggctatga caccaaggtt gaggtttgcg
    23161 gccacggctt ccgcactatg gcctgtagct cattgattga gtcagggtta tggtcgaagg
    23221 atgcggtgga aaggcagatg agccatatgg aacgcaattc ggtgagggcg gcgtatatcc
    23281 ataaagcgga acatctggat gagcgcaagc tgatgttgca gtggtgggct gattttctgg
    23341 atgcgaaccg ggagaagggg attacgccgt ttgactatgc gaagatcaat cggggtaatg
    23401 gcgagtgagt tatctaaaag cgcacattcg cctctaaatt gaaagctgct gacttattca
    23461 aacgaggaat tggtcagcag tgccaaatag ccgagttttc actaaggtaa gcctttttat
    23521 tgaacctaat cggcagtaat gctccaccta agcctaaatt taaagtcatg attacttata
    23581 aaaggggagt ggacccttga ttgagcccta catcgtaaca aaggtacagc aattcggcta
    23641 catcaggagt gatagtcaat tagttaatct aataattaat tgaatctaaa taattaaatt
    23701 tcttacgtaa aattgaccaa tttactggtg gtgtaagccg caatatctta atgtaatcag
    23761 tataaagggg cttgatttct tttaaagata gtatccgtat gaattttaag aaaaaactcg
    23821 aagagcattt taagcagttc gaagcatccc cagtgttatt tgtcggctcc ggtgtgtctc
    23881 gtcgttatct tggtgtgcca tgttggcaag accttttgaa gcattttgct gaggcaattg
    23941 gggaaaatca tataaaatta aaaacaaaat ctaatggtga cttacccgaa tatgcacaat
    24001 tattggtgtc tgcatatgct gagaaatggt gggatactga agaaggacaa ttagcattaa
    24061 gtgaaaaaga acaggagaaa actttcatta acgaacagtc t
//
LOCUS       DAJTYD010000047        23938 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTYD010000047 DAJTYD010000000
VERSION     DAJTYD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.047"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..676)
                     /gene="yddG"
                     /locus_tag="OO585_001702"
     CDS             complement(<1..676)
                     /gene="yddG"
                     /locus_tag="OO585_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6629327.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFT"
     gene            908..3955
                     /gene="fdnG"
                     /locus_tag="OO585_001703"
     CDS             908..3955
                     /gene="fdnG"
                     /locus_tag="OO585_001703"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1493..1495,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6629328.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            3968..4852
                     /gene="fdnH"
                     /locus_tag="OO585_001704"
     CDS             3968..4852
                     /gene="fdnH"
                     /locus_tag="OO585_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6629329.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEAPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            4845..5498
                     /gene="fdnI"
                     /locus_tag="OO585_001705"
     CDS             4845..5498
                     /gene="fdnI"
                     /locus_tag="OO585_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6629330.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(5549..5833)
                     /locus_tag="OO585_001706"
     CDS             complement(5549..5833)
                     /locus_tag="OO585_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6629331.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(5979..6989)
                     /gene="adhP"
                     /locus_tag="OO585_001707"
                     /pseudo
     CDS             complement(5979..6989)
                     /gene="adhP"
                     /locus_tag="OO585_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7123..8820)
                     /gene="maeA"
                     /locus_tag="OO585_001708"
     CDS             complement(7123..8820)
                     /gene="maeA"
                     /locus_tag="OO585_001708"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6629332.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(8977..9114)
                     /gene="sra"
                     /locus_tag="OO585_001709"
     CDS             complement(8977..9114)
                     /gene="sra"
                     /locus_tag="OO585_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6629333.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9216..9431)
                     /gene="bdm"
                     /locus_tag="OO585_001710"
     CDS             complement(9216..9431)
                     /gene="bdm"
                     /locus_tag="OO585_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6629334.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            9777..10208
                     /gene="osmC"
                     /locus_tag="OO585_001711"
     CDS             9777..10208
                     /gene="osmC"
                     /locus_tag="OO585_001711"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6629335.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10264..11190)
                     /locus_tag="OO585_001712"
     CDS             complement(10264..11190)
                     /locus_tag="OO585_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6629336.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11183..12169)
                     /locus_tag="OO585_001713"
     CDS             complement(11183..12169)
                     /locus_tag="OO585_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6629337.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12166..13062)
                     /locus_tag="OO585_001714"
     CDS             complement(12166..13062)
                     /locus_tag="OO585_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6629338.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPRAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13059..14081)
                     /locus_tag="OO585_001715"
     CDS             complement(13059..14081)
                     /locus_tag="OO585_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6629339.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14083..15633)
                     /locus_tag="OO585_001716"
     CDS             complement(14083..15633)
                     /locus_tag="OO585_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6629340.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(15647..16228)
                     /gene="ddpX"
                     /locus_tag="OO585_001717"
     CDS             complement(15647..16228)
                     /gene="ddpX"
                     /locus_tag="OO585_001717"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6629341.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(16486..18885)
                     /gene="dosP"
                     /locus_tag="OO585_001718"
                     /gene_synonym="pdeO"
     CDS             complement(16486..18885)
                     /gene="dosP"
                     /locus_tag="OO585_001718"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6629342.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTDLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(18910..20291)
                     /gene="dosC"
                     /locus_tag="OO585_001719"
                     /pseudo
     CDS             complement(18910..20291)
                     /gene="dosC"
                     /locus_tag="OO585_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(20663..21982)
                     /locus_tag="OO585_001720"
     CDS             complement(20663..21982)
                     /locus_tag="OO585_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6629343.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22113..23648)
                     /gene="gadC"
                     /locus_tag="OO585_001721"
     CDS             complement(22113..23648)
                     /gene="gadC"
                     /locus_tag="OO585_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6629344.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         5931 a         6064 c         6065 g         5878 t
ORIGIN      
        1 aggtaaatgc cgcagagatg agtttaatca tgacgggcgt gctaaatacc atttctggtt
       61 gttgcgtaag aaaatagtaa acccacagac ttgctcccgt tagcaggaca aaaacggtaa
      121 ttccgttaaa tccgcgtgcg tatttattcg ttactgtgca ataggctgcc cagataaacg
      181 caccaataaa cgccaggaaa taactcaatg ggctggtggt gatattattg atgatttcat
      241 cataatgtaa cccattctca ccgcctaaca cccagcagac gccgaccagg gctaataata
      301 atcctggtac aatcagccag ttggttttct gaccattaaa cagaatggca aagagaattg
      361 tcaggctggg ccacagatag ttcaccatac ccgcttcaat cgcctgatga cgggtcgccg
      421 cataccctaa ggaaagcgcc agacagattt catagctgac gaataacaga ctcccggcga
      481 gtaaatagcc tttcggaatc tgccgaatac gcggaaatcc aaccgtgaag attaacagca
      541 gcccgcttaa tgaatagata gcagctgcgc cgccgaccgg gccgagcccc tcgctgacac
      601 cgcgaatcaa tcctaccatc gtgctccaca ggacgatcgc tattagccct atgagcgttg
      661 ctttttgtcg tgtcatgctc gctgttttgt ctctcttgcc gttaaaaatt aagctgaatt
      721 ttatagcatt tttttaactg gcctgtcagg cagtggtgcg tttttctacc gctattgagg
      781 taggtcaatt tgcgcaggcg gattattttg tggcaaacac gtgttctttt tgatttcgcg
      841 caaaaagatt cagaatttta ctgttagttt cctcgcgcag taatatccct gaaaaaagag
      901 gaaagcaatg gacgtcagtc gcagacaatt ttttaaaatc tgcgcgggcg gtatggctgg
      961 aacaacggta gcggcattgg gctttgcccc taagcaagca ctggctcagg cgcgaaacta
     1021 caaattatta cgcgctaaag agatccgtaa cacctgcaca tactgttccg taggttgcgg
     1081 gctattgatg tatagcctgg gtgatggcgc gaaaaacgcc agagaagcga tttatcacat
     1141 tgaaggtgac ccggatcatc cggtaagccg tggtgcgctg tgcccaaaag gggccggttt
     1201 gctggattac gtcaacagcg aaaaccgtct gcgctacccg gaatatcgtg cgccaggttc
     1261 tgacaaatgg cagcgcatta gctgggaaga agcattctcc cgtattgcaa agctgatgaa
     1321 agctgaccgt gacgctaact ttattgaaaa gaacgagcag ggcgtaacgg taaaccgttg
     1381 gctttctacc ggtatgctgt gtgcctccgg tgccagcaac gaaaccggga tgctgacaca
     1441 gaaatttgcc cgctccctcg ggatgctggc ggtagacaac caggcgcgcg tctgacacgg
     1501 accaacggta gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt
     1561 ggatatcaaa aacgctaacg tcgtaatggt aatgggcggt aacgctgctg aagcgcatcc
     1621 cgtcggtttc cgctgggcga tggaagcgaa aaacaacaac gatgcaacct tgatcgctgt
     1681 cgatcctcgt tttacgcgta ccgcttctgt ggcggatatt tacgcaccta ttcgttccgg
     1741 tacggacatt acgttcctgt ctggcgtttt gcgctacctg atcgaaaaca acaaaatcaa
     1801 cgccgaatac gttaaacatt acaccaacgc cagcctgctg gtgcgtgatg attttgcttt
     1861 cgaagacggc ctgttcagcg gctacgacgc tgaaaaacgc cagtatgata agtcgtcctg
     1921 gaactatcag ttcgatgaaa acggctatgc aaaacgcgat gaaacactga ctcatccgcg
     1981 ctgtgtgtgg aacctgctga aagcgcacgt ttcccgctac acgccggacg tcgttgaaaa
     2041 catctgcggt acgccaaaag ccgacttcct gaaagtgtgt gaagtgctgg cctccaccag
     2101 cgcaccggat cgcacaacca ccttcctgta cgcgctgggc tggacgcagc acaccgtggg
     2161 tgcgcagaac atccgtacta tggcgatgat ccagttgctg ctcggtaaca tgggtatggc
     2221 cggtggcggc gtgaacgcat tgcgtggtca ctccaacatt caggggctga ctgacttagg
     2281 tctgctctct accagcctgc caggttatct gacgcttccg tcagaaaaac aggttgattt
     2341 gcagtcgtat ctggaagcga acacgccgaa agcgacgctg gctggtcagg tgaactactg
     2401 gagcaactat ccgaagttct tcgttagcct gatgaaatct ttctatggcg atgccgcgca
     2461 gaaagagaac aactggggct atgactggct gccgaagtgg gaccagacct acgacgtcat
     2521 caagtatttc aacatgatgg acgaaggcaa agtcaccggt tatttctgcc agggctttaa
     2581 cccggttgcg tccttcccgg acaaaaacaa agtggtgagc tgcctgagca agctgaagta
     2641 catggtggtt atcgatccgc tggtgactga aacctctacc ttctggcaga accacggcga
     2701 gtcgaacgat gtcgatccgg cgtctattca gactgaagta ttccgtctgc cttcgacctg
     2761 ctttgccgaa gaagatggtt ctatcgctaa ctccggtcgc tggttgcagt ggcactggaa
     2821 aggtcaggac gcgccgggcg aagcgcgtaa cgacggcgaa attctggcgg gtatctacca
     2881 tcatctgcgc gagctgtacc aggccgaagg tggtaaaggc gtagaaccgc tgatgaagat
     2941 gagctggaac tacaagcagc cgcacgaacc gcaatctgac gaagtggcta aagagaacaa
     3001 cggctacgcg ctggaagatc tctatgacgc taatggcgtg cttattgcga agaaaggtca
     3061 gttgctgagt agctttgcgc atctgcgtga tgacggtaca accgcatctt cttgctggat
     3121 ctacaccggt agctggacag agcagggcaa ccagatggct aaccgcgata actccgaccc
     3181 gtccggtctg gggaatacgc tgggatgggc ctgggcgtgg ccgctcaacc gtcgcgtgct
     3241 gtacaaccgt gcttcggcgg atatcaacgg taaaccgtgg gatccgaaac ggatgctgat
     3301 ccagtggaac ggcagcaagt ggacgggtaa cgatattcct gacttcggca atgccgcacc
     3361 gggtacgcca accgggccgt ttatcatgca gccggaaggg atgggacgcc tgtttgccat
     3421 caacaaaatg gcggaaggtc cgttcccgga acactacgag ccgattgaaa cgccgctggg
     3481 cactaacccg ctgcatccga acgtggtgtc taacccggtt gttcgtctgt atgaacaaga
     3541 cgcgctgcgg atgggtaaaa aagagcagtt cccgtatgtg ggtacgacct atcgtctgac
     3601 cgagcacttc cacacctgga ccaagcacgc attgctcaac gcaattgctc agccggaaca
     3661 gtttgtggaa atcagcgaaa cgctggcggc ggcgaaaggc attaataatg gcgagcgtgt
     3721 cactgtctcc agcaagcgtg gctttatccg cgcggtggct gtggtaacgc gtcgtctgaa
     3781 accgctgaat gtaaatggtc agcaggttga aacggtgggt attccaatcc actggggctt
     3841 tgagggtgtc gcgcgtaaag gttatatcgc taacactctg acgccgaatg tcggtgatgc
     3901 aaactcgcaa acgccggaat ataaagcgtt cttagtcaac atcgagaagg cgtaaggggg
     3961 cgaacagatg gctatggaaa cgcaggacat tatcaaaagg tccgcaacta actccatcac
     4021 gccgccttct caggtgcgtg attacaaagc agaagtcgca aaacttatcg acgtttccac
     4081 ctgtatcggc tgtaaagcct gtcaggtggc gtgttcggag tggaacgaca tccgtgatga
     4141 agtggggcac tgcgtcgggg tttacgataa ccccgccgat ctgagcgcca agtcctggac
     4201 ggtgatgcgc tttagcgaaa ccgaacagaa cggcaagctg gagtggctga tccgtaaaga
     4261 cggctgtatg cactgtgaag ctcccggctg cctgaaggcg tgcccgtctg ctggtgcaat
     4321 cattcagtac gctaacggga ttgtcgattt ccagtcggaa aactgcatcg gctgtggtta
     4381 ctgcattgcc gggtgtccgt ttaatattcc gcgcctcaac aaagaggata accgggtata
     4441 taaatgcacg ctctgcgtcg atcgcgtcag cgtcggccag gaaccggctt gtgtgaaaac
     4501 ctgtccgacc ggggctatcc acttcggcac caagaaggag atgctggagc tggcggaaca
     4561 gcgcgtggcg aaactgaaag cgcgtggtta cgaacatgct ggcgtctaca acccggaagg
     4621 ggtcggtggt acgcacgtta tgtacgtgct gcatcacgcc gatcagccgg agctgtatca
     4681 cggtctgccg aaagatccga agatcgacac ctcggtaagc ctgtggaaag gcgcgttgaa
     4741 accgctggca gcggctggct ttattgccac ttttgccggg ttgattttcc actacatcgg
     4801 tattggcccg aataaggaag tggacgatga cgaggaggat catcatgagt aagtcgaaaa
     4861 tgattgtgcg caccaaattt attgatcgcg tctgtcactg gaccgtggtg atttgcttct
     4921 tcctggtggc gctgtccggg atttcgttct tcttcccgac gctgcaatgg ctgacgcaaa
     4981 ccttcggtac gccgcagatg ggacgcattt tgcacccgtt cttcggcatt gcgattttcg
     5041 tcgcgctgat gtttatgttt gtgcgttttg tgcatcacaa catcccggat aagaaagata
     5101 ttccgtggct attgaacatt gtcgaagtat taaaaggcaa tgagcacaaa gtggcggatg
     5161 ttggtaagta caacgccggg caaaagatga tgttctggtc gatcatgagc atgattttcg
     5221 tgctgttggt gaccggggtg attatctggc gtccgtactt tgcgcagtac ttcccgatgc
     5281 aggttgttcg ctacagcctg ctgatccacg cggctgccgg tatcatcctg atccacgcca
     5341 tcctgatcca tatgtatatg gcattctggg tgaaaggatc gattaaaggg atgatcgaag
     5401 ggaaggtaag ccgtcgctgg gcgaagaaac accatccgcg ctggtatcgt gaaatcgaga
     5461 aggcagaagc gaaaaaagag agtgaagaag ggatataatc tcttttgaac tttaagctga
     5521 aaatggcgct ttaagaagcg ccattttttt attgcgtaac cagacggcgc agtcgcgaca
     5581 catccaccgt tttttcagcc tctgccagac tccaggcgtt ttgcagattc agccacattt
     5641 gcggcgaact gccgatcacc acggaaagtt taattgccat ttctggcgtc aaagctgctt
     5701 ttccggtcag caatcggctt gccgttgagg gcgcaatttc cattgctctg gcaaactcgc
     5761 gcaggctgac attaagttcg tccagtgatt cctgaataat gtcccccggg cggggatgat
     5821 tggccatttt cattagtgtc tttactctca gtagcgttcc ttctctggga acactatcat
     5881 tattgttcct tcctacgcaa caataatcca tttagccatt tcatgttgga gagtgtgcga
     5941 tgctgcgacc cgaacatggg agtcgcagca aagacctctt agtgacggaa atcaatcacc
     6001 atacggccac ggattttgcc ttcttccatc tcggtaaaga tggtgttgat gtccgctaac
     6061 ggacgcaggg cgactttcgg caccactttg ccttcggcgg caaactggaa ggcttcggtt
     6121 agatcctggc gcgtgccgac cagcgaaccg accacttcaa tgccatccag cacaagacgt
     6181 gggatatcca ggctcataga ctccggcggc agaccgacag ccacaacacg accgcctgca
     6241 cggacagcat caactgccga gttaaacgca gctttagcta ctgctgttac caccgcagcg
     6301 tgagcgccac cggttttctc ctgcacaatt taggcggcgt cttcggtgcg tgagttaatc
     6361 gctaaatctg cacccatttc ggttgccagt tttaactgct catcattgac atcaatggcg
     6421 atcactttgg cgttaaagac attcttcgcg tattgcaggg cgaggttacc cagaccgcca
     6481 agaccgtaga tagcaatcca ctgaccggga cgaatttttg acagtttaac ggctttgtag
     6541 gtggtgaccc ccgcacaggt aatgctgctg gccgccgccg agtccagacc atctggcact
     6601 tttaccgcgt aatcggcgac cacgatgcac tcttccgcca tcccgccatc aacgctgtat
     6661 ccggcatttt taactgaacg gcagagcgtt tcgttaccac tgttacagta ttcgcaatga
     6721 ccgcatcctt cgtaaaacca cgccacgctg gcacgatcgc ctggttttaa tgaggtgaca
     6781 cctgggccaa cttctgccac cacaccgatg ccttcatggc ccagaattac cccagttttg
     6841 tcaccaaaat cgccattctt aacatgaaga tcggtatgac atacaccaca acactccatt
     6901 ttcagcaggg cttcgccatg tttcagtgag cgcagtgttt tatccgtaac gtcaacatga
     6961 tgatccttcg taacaactgc agccttcata gttcctcctt ttcggatgat gttctgcata
     7021 gcaggtgagg caaatgagat ttattcgcca ctacccagta tggatgagat ctgaaaaagg
     7081 gagagggaaa tagcccggta gccttcacta ccgggcgcag gcttagatgg aggtacggcg
     7141 gtagtcgcgg tattcggctt gccagaaatt atcgtcaatg gcttgttgca aagcttcagc
     7201 agacgttttc accgccacgc cttgctgctg cgccattttg ccaaccgcaa acgcaattgc
     7261 gcgggagact ttctgaatat ctttcagttc cggtagtacc agaccttcgc cgttcagcac
     7321 cagcggcgaa tactgagcaa gcgtttcact tgctgacatc agcatctcat cggtgatacg
     7381 tgacgcgccg gaagcaataa cacccagccc gatgccaggg aaaataaagg cgttgttaca
     7441 ctgggcgata gggtagattt tatctttcca taccactgga ttaaacgggc tgccagtggc
     7501 gaccagcgcg ttaccttcgg tccaggcgat aatgtcctgc ggtgtggctt ccacgcgtga
     7561 cgtcgggtta gacagcggca tcacgatcgg acgcggacag tgtttatgca tctcacggat
     7621 gatctcttcc gtaaacagcc cggtctgtcc tgagacgcca atcagaatat ctggttttac
     7681 attgcgcacc acatccagca gtgacagcac atcgctgtcg gtatcccagt cactgaggtt
     7741 ttcgcgtttc tgcaccagtt tggtctggaa aggcagcagg ttcggcatct tgtccgtcag
     7801 caggccaaag cgatcgacca taaagacttt ctgccgcgcc gcttcctcgc ttaatccttc
     7861 gcgctgggtc tgggcgatga tcatttcggc aatgccgcat cccgctgaac ctgcaccaag
     7921 gaagacgatt tttttctcgc ttaactgacc acctgccgct cggcttgctg cgatcagtgt
     7981 gccgactgtt accgccgcag tgccctgaat gtcatcgtta aaagaacaaa tttcattgcg
     8041 atagcggtta agtaacggca tcgcattttt ttgagcaaag tcttcaaact gcaacagcac
     8101 gtctggccag cgttgtttca cagcctggat aaattcatca acgaattcat agtattcgtc
     8161 gtcaatgata cgcggattac gccagcccat atacagcgga tcgttaagca gctgttggtt
     8221 gttcgttccg acatccagca ccaccggaag ggtatacgcc gggctgatgc cgccacaggc
     8281 ggtatagagc gacagtttac cgatcggaat gcccatcccg ccgatgccct ggtcaccaag
     8341 ccccagaata cgttcaccgt cagtcaccac aatcactttg atattatggt tcggtacgtt
     8401 ttgcagaata tcgtccatat tgtggcggtt ctggtaagag ataaacacgc cgcgtgaacg
     8461 gcggtagatc tcagaaaaac gctcacaggc tgcgccgacg gttggggtat aaataacagg
     8521 catcatctca tcaagatgat tgtttaccag acggtagaag agggtttcgt tggtgtcctg
     8581 gatgttacgc aggtagatgt gtttgtcgat ttcggttttg aatccctgat actggatcca
     8641 tgctcgttcc gcttgttctt cgatggtttc gaccacttcc ggcagtaacc ccagcaggtt
     8701 gaagttacgg cgttcttcca tgctgaaggc actgccttta ttcaacaacg gaaattccag
     8761 cagtacaggg ccagcgtaag ggatataaag cgaacgctgt ttttttgttt ttggttccat
     8821 gtcactcact cttttttgaa tatccatccc tggcacgctt ttatcgtctt tgctttaccg
     8881 ccagggcgtc ggccccaagt ataaagcaga taaaaacaaa aacaccattg cgcaggcaat
     8941 ggtgtttaat cgtcattgag gactgatggt tatgaattac ttttcagcgg ggcgttttct
     9001 gccggttggg ttatttacca cgctggattt gtcaccttcg gtaactattt tgcgctggtt
     9061 agaaatttta tggtccagtc caagaatatg acgtgcctga cggttcgatt tcattttaac
     9121 tcctcaatcc tgttgctagt tttaaggaca acatcgccgt agcgaagaaa cacgtgctaa
     9181 acccctaaat taggttgccg atcaagcata gcaccttaaa gcgtagggtg ctggccactg
     9241 accacataat tgatcgtttg ctggtagata tcactgagga tgtcgttatc agaagcttcc
     9301 acccatttgg tcagctccat gagaatgtca tcttcagtga caacaccgtg ctctgcccga
     9361 aggccttgct caatggcatt aaccagagcg ggttctgctg ttgaattttc tgcctgataa
     9421 taagtaaaca tagtgattct ccgtgtctgt gtatttatgg tgtctgctac ggatcgcaga
     9481 tttataaagc acattcagca tggcaaatat ttgccgcttt gttgttaaga ttagtcctgg
     9541 ttgatgattt ttatatttta acctcatgat attgataggt attattattg tggtgagttg
     9601 attaatatta atgcaatatc tcttgggtta tatattcccg gttatctatt gtggaaattt
     9661 aatttaagtg cagaagtaat atttctgccg gatttttatt cggaatatcc tgcttatcct
     9721 cgtgctgttt ctcacgtagt ctataatttc ctttttaagc ccacatgaga gcaacaatga
     9781 caatccataa gaaaggtcag gcacactggg aaggcgatat caaacgcggg aagggaacag
     9841 tatccaccga gagtggcgtg ctgaaccaac agccgtatgg atttaacacg cgttttgaag
     9901 gcgaaaaagg aactaaccct gaagaactga ttggcgcagc gcatgccgca tgtttctcaa
     9961 tggcgctttc attcatgctg ggggaagcgg gatttacgcc aacatcgatt gataccatcg
    10021 ccgatgtgtc gctggataaa gtggatgccg gttttgcgat tacgaaaatc gcactgaaga
    10081 gtgaagttgc ggtgccgggt attgatgcct ctacctttga cggcatcatc cagaaagcaa
    10141 aagcaggatg cccggtctct caggtactga aagcggaaat tacgctggat taccagttga
    10201 aatcgtaaag cattgccgga tgacgtgtca ggcgcgtgaa tgcctgatgc gttgttagca
    10261 tctctacagc gcccgccagc accggagctc acgtccgtcc tcccttattg ctaatgattg
    10321 ccggacttca catccgctgg ttgcaagggg gcaacgttcg tagaaaaaac agccttgcgg
    10381 caacgtgcgg tttcccggca gatccgtttt acgtaatgcc cattcttcct ccagcggttt
    10441 atcaatggcg gggagggaat ccagcaataa tcgggtgtat ggatgtgccg gagccgttag
    10501 cacctgttgc gcgtcgccca gttctacaat ctgcccgaga tacatcaccg ccacccgatc
    10561 gctcatatga cgtatcaccg agacattgtg tgaaatcagc acataggtca gcccgtgatt
    10621 ttcctgtagc gttaccagta aattgaggat ctgcgcctgc acggagatat ccagcgcaga
    10681 ggttggctca tcaagcacaa tcacgtcagg ctgcgaagag agtgctctgg caatggcgat
    10741 acgttgccgc tgcccgccgg agaacgcatg aggcaggcgg tcgagatact ccggacgaat
    10801 acccacctgc acagccagct cctctaccaa cgctcgccgc tgttgttcac tactacgctt
    10861 agctatccag agcggttctg tgatgatgcg ccacaccggt aagcgcggat tgagcgaaga
    10921 gagcgggtcc tgaaacacca tctgcataat gcgttgtgag ccagaacgga tgtactgccc
    10981 gtggctcggt tgcagcatac ccattaaaag ctgtgcgagg gtgcttttgc cgcagcctga
    11041 ctcgccgacg atccctaagg tttcaccacg gcggatctgt aaatcaatac cattaatcgc
    11101 atgaacatgt tccgtagttt taccaagcca gtttttacgg gccgggaaat tgatatggac
    11161 gccgcgtaac gttaataacg tgtcagacac ttatgacctc ctgctgcgga taccaacagg
    11221 cgcagcgctg gttgttgtca ccacacgctg tcagcgccgg gacgttttca cactgtgcac
    11281 ctgccgcata gcaacgatcg cgaaaagcgc agccgtcagg caaatgggtg aggtttggta
    11341 ccgtccctgg aatggcgggt aatagctggc gtggtactcc atgttctggt gcacattgga
    11401 gcaaaccaat ggtatacgga tgccggggat gatggataac gtctgccctc acgccgcttt
    11461 caattacgct tccggcatac atcacgtaaa cgctatcgca cagttgcgac accacggcca
    11521 tatcatggct gatgaacaga accgcagttc cactggcgcg ggctttatgt ttaagcaaac
    11581 gcagtacctg caactgcacc gtgacgtcca gcgccgtagt cggttcgtcg gcaataatca
    11641 attgcggctc gcaggagaat gccagcgcaa tcattacccg ctggcgcata ccacctgaaa
    11701 gctcaaacgg atagcgcgac ataacttcca cggcatccgg gatttgcatc tcttccagca
    11761 ggacaatcgc tttagctctg gcttcccgac gacttattgg ttgatgatgg cggatcacgt
    11821 ccatcatctg aagacctatt cgacgtgtcg gattgagggc ggtcatcggt tcctgaaaga
    11881 tcatcgccac tcgtgcgccg cgccactgac gaagctgttt ttcccgagcg ttaagaacat
    11941 cgtctcccag cagtgaaatc tgcccccgat gtacgcaata actgcccgtc ggtagcagac
    12001 gcataatcag cattgcggtg actgatttac ctgagccgga ttctcccacc agaccgacaa
    12061 tttcaccgcg gttaatctgc aaggacacat tgttgagcgc gtgaacatca ccgttaaaac
    12121 cggggaaact caaatgcagc tgttgaatgt ccagaacggg ctgggtcatg actgctttcc
    12181 tcctgctttc gggtcaagca gatcgcgaat accatcacca aagagattaa acccaacggc
    12241 ggtaagcaaa atcgctgccc ttggaaatgc gcaataccac cactgatcga gtacatagtt
    12301 gcgaccattc gctaccatcg cgccccattc agcactcggt tgttgagcac ccaggccaat
    12361 aaatcccaac gtggcggcca ttaaaatcgc gctaccgata tccagcgatg cctgcacgat
    12421 cagcggcggc agggagttac gtaaaatatg ccagctgatc agatgccaac gcgacgcacc
    12481 aaaggttttc gccgcctgaa cataggtata ctggcgtaca actaatgctt gcccccgcgc
    12541 caggcgcaca taaaagggaa ttcgcacaat agcaatcgcc agcatggcgt taaataaact
    12601 cggcccgaga gcggcagcca gtgccattgt cagtaccaac gaaggaatcg acagcataat
    12661 gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca agcacaccgg atagacatcc
    12721 gagtagcgaa ccaatcatac ccgcaatggc aaccacgact aatccggcga gaattgattg
    12781 ctgactgccg accagtacgc ggctgaacag atcgcgtccc acttcatcgg tgccaaacca
    12841 gtgcgccgca gaaggcggca aaaggcgggc ggttaaatca atggcgttgg gatcatgcgt
    12901 cgcaagccac ggtgaaaaaa acatcatcaa tagcattaat acaatgatga ctgcgccggt
    12961 cacggtgagc ggactgcctt tcagcatcca taccagtttt gcaccgttaa atcgcgtttg
    13021 tttttgtggg cgtacggcgg acgtttcctc gcttagcatc attcaccacc tccacgtccg
    13081 atacgcggat caatccacaa atagagcaaa tccaccacca ggttgaccag cacataagca
    13141 aatgaaacca cgacggcaaa gcccatcact gccgggaagt cgagcgcctg tattgatgtt
    13201 actacccatg cacccattcc gggccaggca aaaacggttt cggtgagcac tgcgccatac
    13261 aacaaatcgc ccagcgccag acccaatacg gtaatcgatg ggatcaacgc attgggtagc
    13321 gcataacata aaacgatata ccagccgggc aagccgctgg cccgggcggt acgaatgtag
    13381 tcttcactca attgttccag catcgctgag cggatctggc gagcgacaat tcccaggtga
    13441 acgaacgcca gcgttaatgc cggtaagatg agatgttgca acgcattgaa gaagacttca
    13501 ccgttgcctt caagcagcgc atcgagcaga taaaagccgg taacgtgcgt tggtggatcc
    13561 agccagtcat caagccttcc gccgccggga agaatttgca gatgaccata aaacagcaca
    13621 atgacgccca gtccaagcca aaacgcaggt gtggagattc cggtaatggc cattattcgc
    13681 accagatgat ccagccagcg atttcgccag actgcagaga gtatgcccag cgggatgcca
    13741 atcaggagtg ccagcagcag ggcacaaaaa gccagttcca gcgtcgccgg gaaaaatata
    13801 cgcaactctt ccaacaccgg acgcccggta cgaatggatg ttcccaggtc accatgaaac
    13861 aggtcgctga cgtagcggta aaactgtaca tacagtggct ggtccagtcc cagttgctgg
    13921 cgaatatttt ccacgatagc atcgctggcg cggtcacccg ccagtaaccg cgccggatcg
    13981 ccagggatca gatgcgagat aataaacgta atcacgcaga cgcccgccat cacgagcacc
    14041 agcccccagc agcgttggcg taaaatactc cagaaggtca cgttatttac tcatggtatt
    14101 gatattgaag acctgttcca gcatgggatt gaacacaaag cctttcacct ctttgttcat
    14161 cgccagttgg tagtttttct ggaacaggta cacataagca gcgtcatcaa tgacgatttt
    14221 ctgtgcctgc tggtagtccc gggtacgctg cgtctggtcg gtggtcgcaa gcgcattgcg
    14281 cagtaactta tcgacctcac tgttttcata gaacgagcgg ttacccggca gacctttttt
    14341 gtctgactca aaccagtaat tcataaacat atacgggtcg gcaaaatccg gactccagtt
    14401 gccaatcgca atgtcgtaat cacctttacc cactctgtcg cgcatggtgg cgttcgccag
    14461 cttttccagc ttcacattga tgcccagctt gttgagactg gattgtgtcg ccagagcaat
    14521 aggctcccag ttcggatcct tatcggagta gagaaacgtc aggctggtgg gtttgctcgt
    14581 cactttatcc cattcggctt tggctttcgt ttcgtcatgg ttgtattgca ttgccgtcgc
    14641 atcgtagccc cacatgcctt ccggaatcgg gccgcgcatc tgttttccgt taccactcag
    14701 aatgccgtta accatgccct gataatcggt agaccaggaa atggcccgac gcagatccgc
    14761 ctgattaaga ggcgctttgc tgttattcag atacaggtag gtgacgcgca gtgacggata
    14821 ctctgccaca ttgactttgt tttcctgctt cagggcgttg agttgatcca ccggcagcgc
    14881 atcggcaatg tcaatatcac cacgggagag ctgcaggcga cgggaggcac tttcaccaat
    14941 aatttttacc gatactcgct taaagttcgg tttattgcca gggtaatgcg gatttggcac
    15001 cagaactaat tgctgacctt tttgccagct tttcagcata aatggtccgg aaccggcggt
    15061 attttgcgcg aggaagccgc gggcatcatc cgctgcatgt tcctttaaca ccgccggatt
    15121 gataatggat gcaccgtcat tcgccagcgt gtagaggaac ggtgcgaatg gctggctaag
    15181 ggtaaacttc accgtatgtt cgtcgggagc atcaatcttt aaatctttgg gaaatgcttc
    15241 tgctggcccc tggccgattt ttagtagccg ctcaaaagag agttttactg cttcggcagt
    15301 gaccggtgtg ccatcggcaa atttagcatt gtccttcagg gtgaacgtcc actctttttg
    15361 atcgtcagac gctttccagc tacttgccag atcgccttca acgtcggttg agcctttatc
    15421 accgtccgtt ttgtactgaa ccagccgctg ataagacggg taggtcaccg tccagtcgtt
    15481 attatctatt gttaccgccg gggcgagggt ttgtggatcg gcggccttac caatcaccag
    15541 catatctttc ggtacggcgg cgtgcgcaac cgggaaagtt gtggcaagga cgagcgcgag
    15601 caatgtagga cgaaacggta tcgatctctt catgacagtt tcctgattaa ctgacgtgct
    15661 gtgtgccagg ggatataaaa caggtgaatt gatcagcgag cagtgggtaa ctcgctgcct
    15721 ggggtaattc gaagtgccac cattcgctgg agataccgac aaaaccgcca ccagtcataa
    15781 tcgcattcag caacagccga ttgcgctgag cggcgggcgg gacggaaggg tgataggcat
    15841 gggagcgctc gtgcatttca tcgaaccctg cgcccatatc gaggatgttc ccgtgctcat
    15901 cacgaagcgt caggtcgatc gccgtgccac ggctgtgatt agaaccgacc gtcacatcaa
    15961 caacatattg cgggtctggg caggcttgcc acaacatcgc ctgtgcttgt tgtgggcgat
    16021 acgcatcgta aatcaccagt tgtaagcctg acagctgggc gatactgata cttttcgcca
    16081 gcgcggtaat cgcatccttg tgtagcagac aacgcgcttg ctgataaata gctttacctg
    16141 tgatgttatc agcgcaggcg tatttcaatt cgatctccag atcagggaag attacggcta
    16201 aatcaaccag ttcggtggta tccgacataa acggttcctg tataagacaa aaattgctgc
    16261 gctttccgct tatgcagatc tcatgccatg ccgggataag cgccagaatg ctggcttaaa
    16321 gttatatttt ttaacttttg atcaacattt gtgcagcgta gtgcagtttt ggtgcaagag
    16381 gggaagttaa ggaaggaatc tcccggaatc gtagatgaaa tcacagtatt taagtgacgg
    16441 tgtcacgtta aatgaaaacc cgcgagtgcg ggcgagagga atttgtcaga ttttcagcgg
    16501 taacacgctg ctcatccagc ctggaatttc ttcggcgggt agggggcggg aaaagaaata
    16561 tccctgaata acgcgacagt ggatcttgcg tagcatctca aattgctctt tggtttcgac
    16621 gccttccgcc acgacggtta aattgaggct ttgcccaatg ctggtaatgg cttcaagtaa
    16681 ggcaaggatg cgtttttcgg tcagacaacg atcgacaaaa cttttgtcaa ttttgatttc
    16741 cgttaccgga agactgacta agcgggataa tccggaaaag cccgtaccaa aatcatctac
    16801 cgataagccc acgcccatat cacgcaggat ctgaatgcgc ttaaagattt cggtatcgtg
    16861 ttccatcatc atgctttccg tgatttctac cgtcagctgg tggccgtcaa taccccaggc
    16921 gtgcattgca tcagacacct gattaggcag ttgattactg cgaaagtgca gtgccgacaa
    16981 gttaacggaa agcgccggga tatgaatatt ctggctacgc cattctgcta actgacggca
    17041 agcttccgcg atgacccagc gcccaatatt ttcgatttca ccaatctctt ctgcgagagg
    17101 aataaaccgt gaagggggca catgaccatg ctggggatcg tgccagcgcg caagggcttc
    17161 gatgccgtac agttcacccg tttctgcgaa gatttgcggc tggtaaacaa gtttcagttg
    17221 gttattgcta atcgcttctt tcagcgctgc gcctaaaacc aaacgctctt ttaccatttc
    17281 gttcatcgcc gggctgaaga actgccagcc gttaccgcca ttcttgcgaa tataatctat
    17341 tgcattgtga gcagtggaga gtaggtaatc gtggttttta cccacgtcgt agctgatgcc
    17401 aatactcaag gtaagcggga atggtttatc gtcaatcatt atcggcttgc tgaccacatt
    17461 ccgtagctca tcggcgatct gggtaatgtt actgacgtcg ttttccaggc tcacgaggac
    17521 aaactgcgta ccttcgatac gacagagata ctgatccggt ttgagttttc cacgaaagcg
    17581 attgaccact tccagcaatg cctgatcggc ccacgcatag ccaaggctat caatcacatc
    17641 ctgaatatgg tcaacaccga tgagatacac caccggagag acggctttgt cgaccaggtc
    17701 atcgaggtaa ttgtgcaggt tattgcgatt tggcagatcg gtcatcggat caaattggat
    17761 gagttgttca atatgctggc ggcttttttc ctgttccagc gccagcgcgg ccatatgctg
    17821 actgatatct gccacgcgtt cgataaaggc gctggtttct gctcctgacg aggttttaat
    17881 ttgcaggatc cccgcaggcg cgccatcacg ctgacgaatg gtcgctgacc agctttgcgc
    17941 attttgaact tctgcaccgt gcgaagatga cgcccagtgt atcggcatcc cgttgcgcag
    18001 tgcgaacagc gaaacatgcg attcgttgag aacagattcg atgttacgac aaatgatttc
    18061 ccccatttca tgaaatggcg ggctgctgca catggcggcg agaatattgc cttcaagctg
    18121 gcggatttgc cgttcttcgg tgatatccga gaaagtcatt accaggttct gcagatgcgc
    18181 gagcacgtca taaaccgggc tgatagaggc tttaatccag attttttcac cggtgcgcgt
    18241 caacagcaga aattcgtcct gatcgcgggc ggttttccat agcaactgtt gtaaacgaat
    18301 gcggttatcg gcagggaatt caggaatgtt caggagtgta tcgggctgca taccgctggc
    18361 ttcgctaatg cagtaaccaa acatttcggt aaatgcgcga ttgcactgca caatatggcg
    18421 ttccggatcg aggacaatca ccggtcggtc gagatggtca acggcaataa tcaattgtcg
    18481 ggtctgttct ttttgcgcca tttctacgct ggcatcccgt accagcgcca ggtaataaac
    18541 tttcccctcg gcgctcactt tcgatagcgc aaaacgggtc cagattttac tgccgtcttt
    18601 tttctccagc tgcagctccc gactcatccc ctcgacgcgc gctttaccac cttcacggtt
    18661 atgacgaatg tattcaggat gcgcaggacg caaatcccgc ggaatcagca tatcaatgtt
    18721 attgccaatg acttcttcac gtttgtaccc ccagagcttc tctgcggcgg ggttgaaaaa
    18781 catcacttca tcattttcgt taattaacac cgcgcccatc atattttgtt caagggcggg
    18841 gaaaaaaatg ccatcggcgg cattttccgc atcggttagc ttcatgatta cctctgcatc
    18901 ctggcgcatc taaagactgg ctttccagag ttcaacacgg tttctacctc gtcttttggc
    18961 gatatacaga gcttcatcgg ctatttgaat gaggcgctca tagtcaggat gaccattaaa
    19021 catggcggca ccgatggaaa gtgagagggc aatatcctcg ccgtttgcgg ctttcagttt
    19081 ggttttctcc acccgactgc gaatacgttc tgcggtacgt aacgtttcgt tttcagaagc
    19141 ttcagtcaaa acaatgataa attcatcgcc cccgtagcga aaaacataat cactactgcg
    19201 gacgttgtca taaaaggcct gagagacttt acgcagaatt tcatcgccag tgttatggcc
    19261 ccacgtatcg ttgatctctt tgaatttatc aacgtcaata atcagcactg acagcggtgt
    19321 accggtccgg ttggcatggg caatttcgcg tttgaagata gtcggcagga agcggcggtt
    19381 aagtaatttc gtcagtacat ccataccgac ttcgtggcgc gatacttctt caaacaattc
    19441 acgcagcaag gtaataattt gcgatacagt atttcttatc tgtaataaaa atttcacccg
    19501 cagacttctg ttattcaaat tcctggtgtt acgcatggtt tgattgaata taccgtcgaa
    19561 atcctgaatc agacgggaga tatgcctact tcggcaatgc cactaaaata atgtcgacct
    19621 ttatggttaa accacaggcc aaaatcagcc tggcttaaag gcaaactact gcctaaatca
    19681 gaatccagca ggattttata gataatatct atttcccatg aaagtattga ggctatttgc
    19741 cgttcttttt cttcttcggc gttttctagt aacgagaaga tacgatagtt ttcatcttcc
    19801 tttgaggcac tactgtcact aaaggtaaac gcgcgggtca tcacttccat agcgatatca
    19861 atactgttaa tcgagaaatg gtagacctga agtttttctg cggcagaata atccgaagag
    19921 aagattaccg gatagaggat ttttttcagc acccgaaacc ccatctcgac aatttctacc
    19981 ggaattccta tgcgggcatg cacttccgcg acggtatgct ggatttgtat tagcctttcg
    20041 acatcgtcaa cctgggcaga aagcacgtta ataatccagc gttccatcgc acttttcaac
    20101 tgccgctcaa cttgttcatt actcaagaat tcttcggcat gcgggtcgat gcggacaatt
    20161 cgataaaact cgatactcag ataatgagca tgcgcaacgg caatttccgc ggctttagca
    20221 cgaatgagtg gatctgcccg ttcgacaagt ccggtccact catctttcat tcttttaaaa
    20281 tacatctcca taattcacac ccttataagg ctgggaaatc agacggaatc aaaacgcaac
    20341 gtgcgagatc gactaactgc accatattct cctgaaatat gaagatatac tgaaaagaaa
    20401 taagcgactt aggacagttt caatctacgc tactgttctt cagaagagta tagcccatcg
    20461 taattatttt tcggtgacag cgaatatcgt atggtttttc atattcatac atttttatta
    20521 gggatttata gctgtttaac taagtgtggt taattgcgtg gttaatttga cttatgtaag
    20581 catgattact agtgggatat ttcacaaggt gtaacaagcc ggtgggtaaa gcaccggctt
    20641 gttacaaagt aagcatggga gtttaactgc cccaacgact ttgcagatag ctgaccgctt
    20701 gttgagtctg cggtttattc agatagtcct cacggaacaa gatggtgccg ctaatttctg
    20761 gtaccgcatc gttaagatcg agctgctttt tcagttccgg tacgccgccg ttaatcatcc
    20821 agtctggctc tatctttgaa ggttcaccca ctttatagaa ggcgataccg atatacaggc
    20881 gggtcctggt cggtttaacg acatccgccc accattttgc caacacgtca taacgcgcgg
    20941 cactccgtga gaacggccag taaatttggg gagcaatgta atccagcaat ccttgttcca
    21001 cccatcgacg ggtgtcagcg taggattcgt cataggctgc cgcgcctcgg gtatcggaac
    21061 cgagcggatc gtgtgatcgg ttacgccaca cgcctgccgg gctaacgcca aattcgactc
    21121 ccggcttaat gcttttaatg gtgtgcgata cttttgtaat taactgctga gtattgttgc
    21181 gccgccagtc tgcttttgac gcaaatgcgc ctccgtattt acggtacgtt tcgttatcat
    21241 ttagccgtga acccggtgac tccgtataga aatagtcgtc aaactgcacg ccatctaccg
    21301 gatagcggga aaccacttcg gcgactattg atgtgatcca gtcctgaacc tcagggatgc
    21361 ccgggtcgag gacaaagcga tcgccagacg ttctgatcca gtcacggtgt tgcacataga
    21421 cgctcgccgg ttgttgagac agagtgctat tcagttccct gatagtaccg ggcttcgtat
    21481 taaccgatac gcgatagggg ttaaaccagg cgtgtacttt catcccacgc ttgtgggctt
    21541 cgtcgagcat aaattgcagc ggatcgtaac ccggattttc accaatctta ccggtcataa
    21601 gatcggacca cggcaaaatt tttgatggcc acagggcggt accgtccggc ttgacctgga
    21661 aaaagaccgt gtttataccg agacgttgca gatgatccag tttgtcgatc atcgcctgtt
    21721 gttgtacacg ggcccggctg gtggggttac taatgttaac cgaggaaacc ggtggccagt
    21781 cgaggcgaga caccgtggcc agccagatgc cacgcatcgg ttgtgacgat tgttgcgtcg
    21841 tggctggtgg ctttgaacct gctggtggtg tcaccatgga ctctggaggc gtgcttttac
    21901 aactacacag taaaagtgcc agtgcaacca gtatcgctgg tcttctaatc gttaatttct
    21961 tgtttcggga gcagatatcc atatgtgctg gtttccggta aacagatgtg cgctcattct
    22021 cgtacttatc ccggttaagt caatacgaca gcaagcacga aaaagggagc gatgaattat
    22081 cgctcccttg tcttataacc attcagacat ggttagtgtt tcttgtcatt catcacaata
    22141 tagtgtggtg aacgtgcacg cgggtgcagg aagaagtgac cttttggtgc gttctgactg
    22201 ttgattggct ccagagtgac gccggtattc gctttgcctt tacgatcatg aacagcatag
    22261 agaataaagg gcagggcaag taccaccagg aaactaacga ccagtaattc aacatacata
    22321 tcggtagagt caccctggat gttatccggc ggcaggaagg aaacaataaa cgccataatt
    22381 gaagtcagca gaccgacaat tgccacgacc agttttaccc ctttaccacc agggatatta
    22441 aatgtgcgtt ttaagtcagg atgtttaaga actaacacaa tgtagccaat aaacagcatg
    22501 aaataagcac acagataaat caccaccgtc agcgccagtg cgatcaggaa ggacatgttg
    22561 ttaccgccac cggtattggt gaggatgatc aacgcgatag aagtaatcac cagctgcgaa
    22621 atgaccagcg ttaccggtac gccatttttg ttcattttag cgaatgccgc tggcagcagg
    22681 tttttctgcg ctgtgacata catcccgcga gaaggaccaa caatccagga ggcgatttcc
    22741 gccagaacac ccagcaacag cagtgcggag atcacgcgaa ccgtccactc aatttccggt
    22801 gccacatggg acatcagaac ggtaaaggtt tgcattaccc ctgcggagag gttgatttca
    22861 ttacccggaa tgaccatcgc aatagataaa ccgccaacag agcttaagca gattgccgcc
    22921 accatcagca gtaacatagc cagtggatag tcgcgcccgg ggttgctcat ttcattgacg
    22981 tgagttgcgg aagcttctac gcccatataa ctcaaaatga aggcaacaaa tacaaccagt
    23041 gtgcccactt tagagaagtc agggaagaag gtcttcgaat ccatttcgat agcaacgggg
    23101 gcaccggagt gcagataaat agccgctaat gcgatcaaaa taaatgcagg taacaggata
    23161 ccggcgaaga agccaacttt agcaattcgc gccgtgtatt tcgtgccacc aaactgcgtt
    23221 aatgccagcg cccaaagaat gatgagtgct gcaatagttt tggtaatggg gtcttcattc
    23281 agcgctggcc atttcaggat gtaggagagt gcccctaaca cgaaatagag catcggaata
    23341 aaaccaatgg cgatttgcag atagccaaat gagatcgctg caaatcccca tctcggcccc
    23401 agagtatttg atacccaggc gaagacacca ccttcttccc agccgtcgac ggtggccatt
    23461 tccgcagcac aaagtcccac gggaataaac cataaaatcc cgcctaatag caggaagaag
    23521 actaatgaaa agcccgatgt tgcgaaggta gggtattcat aaacagccat taccatcgat
    23581 gccgttatgg caaagaatcc gagtaatgtg agctgcttag ctttacctgt ctgtactgat
    23641 gtagccatat tatcccccta aaacggtatt cctgtcggaa ccgcacctgt tttgttctga
    23701 agcgtattca gaacaatatt ttccgttgct aatgccagtg aacagacttt ggaaattgtc
    23761 ccgaaacggg ttcgtttcgg gacaccgtta ccgttaaacg ttatcaggta tgtttaaagc
    23821 tgttctgctg ggcaataccc tgcagtttcg ggtgatcgct gagatatttc agggaggctt
    23881 tgtagtcttc cagcaacagt tcagcaaagt ccatttcgaa gccgcgacga cacataat
//
LOCUS       DAJTYD010000048        23717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTYD010000048 DAJTYD010000000
VERSION     DAJTYD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.048"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            75..302
                     /gene="feoA"
                     /locus_tag="OO585_001722"
     CDS             75..302
                     /gene="feoA"
                     /locus_tag="OO585_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6629345.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            319..2640
                     /gene="feoB"
                     /locus_tag="OO585_001723"
     CDS             319..2640
                     /gene="feoB"
                     /locus_tag="OO585_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6629346.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2640..2876
                     /gene="feoC"
                     /locus_tag="OO585_001724"
     CDS             2640..2876
                     /gene="feoC"
                     /locus_tag="OO585_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6629347.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3079..3957
                     /gene="rpnA"
                     /locus_tag="OO585_001725"
     CDS             3079..3957
                     /gene="rpnA"
                     /locus_tag="OO585_001725"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6629348.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(3986..4756)
                     /gene="bioH"
                     /locus_tag="OO585_001726"
     CDS             complement(3986..4756)
                     /gene="bioH"
                     /locus_tag="OO585_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6629349.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            4794..5477
                     /gene="gntX"
                     /locus_tag="OO585_001727"
     CDS             4794..5477
                     /gene="gntX"
                     /locus_tag="OO585_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6629350.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5536..6111
                     /gene="nfuA"
                     /locus_tag="OO585_001728"
     CDS             5536..6111
                     /gene="nfuA"
                     /locus_tag="OO585_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6629351.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6472..7788
                     /gene="gntT"
                     /locus_tag="OO585_001729"
     CDS             6472..7788
                     /gene="gntT"
                     /locus_tag="OO585_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6629352.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(7833..9917)
                     /gene="malQ"
                     /locus_tag="OO585_001730"
     CDS             complement(7833..9917)
                     /gene="malQ"
                     /locus_tag="OO585_001730"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6629353.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(9927..12320)
                     /gene="malP"
                     /locus_tag="OO585_001731"
     CDS             complement(9927..12320)
                     /gene="malP"
                     /locus_tag="OO585_001731"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6629354.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEISEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            12932..15662
                     /gene="malT"
                     /locus_tag="OO585_001732"
                     /pseudo
     CDS             12932..15662
                     /gene="malT"
                     /locus_tag="OO585_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(15705..16721)
                     /gene="rtcA"
                     /locus_tag="OO585_001733"
     CDS             complement(15705..16721)
                     /gene="rtcA"
                     /locus_tag="OO585_001733"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6629355.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(16725..17951)
                     /gene="rtcB"
                     /locus_tag="OO585_001734"
     CDS             complement(16725..17951)
                     /gene="rtcB"
                     /locus_tag="OO585_001734"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6629356.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18140..19738
                     /gene="rtcR"
                     /locus_tag="OO585_001735"
     CDS             18140..19738
                     /gene="rtcR"
                     /locus_tag="OO585_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6629357.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19720..20478)
                     /locus_tag="OO585_001736"
     CDS             complement(19720..20478)
                     /locus_tag="OO585_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6629358.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20495..21325)
                     /gene="glpG"
                     /locus_tag="OO585_001737"
     CDS             complement(20495..21325)
                     /gene="glpG"
                     /locus_tag="OO585_001737"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6629359.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21370..21696)
                     /gene="glpE"
                     /locus_tag="OO585_001738"
     CDS             complement(21370..21696)
                     /gene="glpE"
                     /locus_tag="OO585_001738"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6629360.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            21886..23391
                     /gene="glpD"
                     /locus_tag="OO585_001739"
     CDS             21886..23391
                     /gene="glpD"
                     /locus_tag="OO585_001739"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6629361.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23443..>23717)
                     /locus_tag="OO585_001740"
     CDS             complement(23443..>23717)
                     /locus_tag="OO585_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629362.1"
                     /translation="LYISPEKSALLGNIRNFFLTFVWPPLNFSPFWPQPWTDFVGPIS
                     AQLGFALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5539 a         6481 c         6038 g         5659 t
ORIGIN      
        1 atcatctggt ctcatgtcgc tgtcaaacgc cccatgaggt agttatccag ttaatgagaa
       61 acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact ggcttttccc
      121 gtgaaatcag cccggcatat cgccaaaaac tgctttctct tggcatgtta cctggctcct
      181 cttttaatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa acccgtcgtg
      241 tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg gtttcctgtt
      301 aatacggtga taacaacaat gaaaaaatta accataggct taattggtaa tccaaattct
      361 ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg taactgggct
      421 ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca ggtcacgctg
      481 gtggacctac ccggcaccta ttctctgacc accatctcat cgcagacctc gctcgatgag
      541 caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa cgtggtggat
      601 gcgtctaacc ttgagcgtaa cctgtacctg acgctacaac tgctggaact cggcattccc
      661 tgcattgtgg cactgaacat gctcgacatt gccgagaagc aaaatattcg tattgaaatt
      721 gatgctctgt cggcgcgtct gggctgtccg gtgatcccgc tggtttcaac ccgtggtcgc
      781 ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa tgtggaactg
      841 gtgcattacg cacagccgct gctcaacgaa gcagattcac tggcaaaagt gatgccttcc
      901 gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg cgatatctac
      961 agccgcgcct acgccggtga agcgtcgcag catctggatg ccgccctcgc ccgtctgcgt
     1021 aatgagatgg acgatccggc gctgcacatt gccgatgcgc gttaccagtg cattgctgcc
     1081 atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac cactgcggta
     1141 gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt gatgtacctg
     1201 atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttga cgtcggctcc
     1261 gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt cccggactgg
     1321 ctgactatct tcctcgccca gggcctgggt ggcggcatta acaccgtgct gccactggtg
     1381 ccgcagattg gcatgatgta cctgttcctc tccttccttg aggactccgg gtatatggcg
     1441 cgtgcggcgt ttgtgatgga ccgtctgatg caggcgctgg gcttgccggg gaaatccttt
     1501 gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggtgc acgtacgctt
     1561 gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg
     1621 cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg tgcgctggcg
     1681 gtcttctcgc tgtatatgct gggtattgtg atggcggtgc tgactggcct gatgctcaag
     1741 tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agttgccggt ctatcatgta
     1801 ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaaaggctt cgttctgcgt
     1861 gctggtaaag tgatcatcat cgtcagcatt ttcctgagcg ctttcaacag cttctcgctg
     1921 agcgggaaaa tcgtcgataa catcaacgac tcggcgctgg cgtccgtcag ccgggtgatc
     1981 accccggtct tcaagccaat tggcgtgcat gaagataact ggcaggcaac ggttggcctg
     2041 tttacaggtg ccatggcgaa agaagtggta gtgggtacgc tcaacaccct ctacaccgca
     2101 gaaaatattc aggacgaaga gttcaatccg gcagagttta acctcggtga agagctgttc
     2161 agtgcgatag atgaaacctg gcagagcctg aaagacacct tcagccttag cgtactgatg
     2221 aaccccattg aagccagcaa aggcaacggc gaaatgggta ccggggcgat gggcgtgatg
     2281 gatcagaaat tcggtagcgc agcagcagct tacagctacc tgattttcgt cctgctgtat
     2341 gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg ctggatgggc
     2401 ttctccatcc tgtgggggct gaatatcgct tactcgctgg caacattgtt ctatcaggtc
     2461 gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt tatcctgttt
     2521 aacatcgtgg ttatcggtct gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg
     2581 gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga ttgccattaa
     2641 tggcttcact tattcaggtg cgcgatttgc tggcgttacg gggccgtatg gaagcggccc
     2701 agataagcca gacattgaac actccacagc caatgattaa cgccatgctg caacaactgg
     2761 aaagtatggg caaagccgtg cggattcagg aagaacctga cggctgcctc tctggcagtt
     2821 gtaaaagctg cccggaagga aaagcctgtc tgcgcgagtg gtgggcgctt cgttaacctt
     2881 actccatcgc ctgttttgga aagcccggta tgcgtctgca tccgggcttt tttgcgtgcg
     2941 gctttccata aaaatgcaac tcttgcagca cggcgtaagt tcctttgaaa gcatctcgca
     3001 gggatgaaaa ctcgctaata cacaggtgtg gagtggcgcg tagagtcgcg gcattcaaac
     3061 aacaggtgaa ggaacgccat gagcaaaaag cagagttcca ccccacacga tgcgctgttc
     3121 aaactctttt tacgccaacc ggacacggct cgtgattttc ttgcgtttca tttaccggca
     3181 cccattcacg cgctttgtga tatgaaaacc ctcaagctgg agtcgagcag ctttattgat
     3241 gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca
     3301 ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat cgcatttcgc
     3361 atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata caaaacgttg
     3421 ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc ctattcgctg
     3481 tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc agctttatgc ctccgcattt
     3541 ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg acgtatggcg
     3601 ctgctggagt taattcaaaa acatattcgt caacgcgacc tgatggggct agtagagcaa
     3661 atgggctgct tattaagtag tggatacgct aatgacagac aaatcaaagg gctgtttaat
     3721 tacatactgc aaaccggcga cgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa
     3781 cgttcaccga aacacaagga gagtttaatg acaattgcgg aaagattgcg gcaggagggg
     3841 gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt ccctcttaca
     3901 gacatcatgc gctttaccgg ggtgtcagaa gaagagttgg ctgcggcgag ccagtaaatt
     3961 tctgtctcgc catttcaaaa gttgcctaca ccctctgctt caacgccacc agcaggtgac
     4021 aaaactcgac cggatgcgaa ataaacggcg catgggccgc tttggcgaag atatatgatt
     4081 tgctgcgagg ccagagttta tccagcatcg gcaccacttt gcgcggcacc agaccatcga
     4141 gatagccata caatcgcaaa aacggcatgg gcacgttttg cagcggctga cggagatcaa
     4201 ccgttttcag gatttccagc ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca
     4261 gaacggtttt cttcaacgcc cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta
     4321 acgccaggaa ccgctccact gtacgctgaa aatcatcact gagttgctgc tgaaatcccg
     4381 ccagcacgtc cggttttatc cccggccaat cgtcacgagc actaaaacaa ggtgacgacg
     4441 ccacggtgac cagcgcctga acacgctcgg gatgggttaa cgcaatctgg cttgccacca
     4501 gcccgcccag actccagcct aaccaaatgg ctttatcagg tgcctgttgc agcacggctt
     4561 cggccatatc agcaagtgac agcgcaccaa atccctggct gcgcccgaag ccgggcagat
     4621 caacaaggtg cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg
     4681 cattcagtcc ccatccgtgc agcagcacaa gatgaacatt cccctgacct ttggtctgcc
     4741 accagatgtt attcatccgc tattgttctc ttttgactta caaggatgaa catatgctaa
     4801 cagtaccggg attatgctgg ctatgccgaa tgccgctggc gttaggtcat tgggggattt
     4861 gttcggtctg ctcacgcgcc gcccgtacag ataaaacgtt atgcccacaa tgtggattac
     4921 ccgccacaca ctcacatctt ccctgcggtc gctgcctgca aaaaccaccg ccctggcaaa
     4981 gactggtcac ggttgccgac tatgcgccgc cgttaagtcc gcttatccac cagcttaaat
     5041 tttcccggcg cagtgaaatc gccagcgccc tgtcacgtct gttactattg gaagtcttac
     5101 acgctcgtcg caccaccggg ttgcaattgc cggatcgcat catcagcgtt ccgttatggc
     5161 agcggcgtca ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag ccgttatcac
     5221 gctggttgca ctgccgatgg gatagcgaag ccgtcacacg aacacgggcc actgcgaccc
     5281 agcattttct cagtgcccgg ctacgcaagc gcaacctgaa aaatgccttt cgtcttgaat
     5341 tgcccgtgca aggtcgccat atggtgattg tggatgatgt cgttaccacc ggaagtaccg
     5401 tcgcagagat tgcgcagttg cttttacgca atggtgcggc gactgtccag gtctggtgcc
     5461 tttgtcgaac cttgtagagc ctcgatgata ggcgtattat aaccaactaa aatagtcaac
     5521 tattaggcca ttactatgat ccgtatttcc gatgctgcac aagcgcactt tgccaaactg
     5581 ctggcaaacc aggaagaagg gacacaaatc cgcgtatttg tgattaaccc tggcacgcct
     5641 aacgctgaat gtggtgtttc ttattgtccg ccggatgccg tggaagccac cgacactgcc
     5701 ctgaaatttg acctgctgac cgcgtatgtt gatgagttaa gcgctcctta cctggaagat
     5761 gcagagatcg attttgttac cgaccagttg ggttctcagc taacactgaa agcgcctaac
     5821 gccaaaatgc gtaaagtggc agacgatgca ccactgatgg agcgcgtgga gtatatgctg
     5881 cagtcgcaga tcaacccaca gcttgctggt cacggtggcc gcgtttcact gatggaaatc
     5941 accgaagacg gttacgccat tctgcaattt ggcggcggct gtaacggttg ttccatggtc
     6001 gatgtgacgc tgaaagaagg gatcgagaag cagctgctaa acgaattccc ggagctgaaa
     6061 ggtgtgcgcg atctcaccga acaccagcgc ggcgaacact cctactacta agttatcccc
     6121 tcttttgtag attgcccgat gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt
     6181 gccagacgtg ggcgcagcat tgctttggca aatccctccc ttcgtttctc cgcgataata
     6241 tgaccaacct ctcataattt aaatttactc cgctctggtg attctcaaac gccagatgtt
     6301 accggtatca ttcacatggg taccaaacat actcctgaca tctgactaca ataattagtt
     6361 ttagtgggta tcagtcgtgg tgccgcaata tctctgttcc cgattgggat aattagagtt
     6421 tgtcgtcaga aaattgacgt tacccgtaac aaatgaaagg ccaggtaaat catgccatta
     6481 gtcattgttg ctatcggtgt aatcttgttg ttgctcctga tgatccgttt caaaatgaac
     6541 ggcttcatcg ctctcgtcct cgtggcgctt gctgttggat taatgcaagg gatgccgctg
     6601 gataaagtta ttggctccat caaagccggt gtcggcggga cgctcggtag ccttgccctg
     6661 atcatgggtt ttggcgcgat gctgggcaaa atgctggcag actgcggtgg cgcacaacgt
     6721 atcgccacca ccctgattgc caaatttggt aaaaaacaca tccagtgggc ggtggtactg
     6781 accggtttta ccgttggttt tgccctgttc tatgaagtgg gctttgtgct gatgctgccg
     6841 ctggtgttta ccatcgcggc ttctgcgaat attccactgc tgtatgttgg tgtaccaatg
     6901 gcagctgcac tgtctgtgac ccacggcttc ctgccaccgc atccgggtcc gactgcgatt
     6961 gccaccattt tcaatgccga catgggtaaa accctgctgt acggtactat tctggcaatc
     7021 ccgaccgtga ttctcgccgg tccggtttac gctcgcgtgt tgaaaggtat cgataagccg
     7081 atccctgaag gtctgtacag cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc
     7141 gtcagcgtct ggacctctct ggtgccggta gtgctgatgg cgatgcgtgc gattgccgaa
     7201 atgatcctgc cgaaaggtca cgctttcctg ccggtagcgg agttcctcgg tgacccggta
     7261 atggctacgc tgattgccgt gctgattgcg atgttcacct ttggtctgaa ccgtggtcgt
     7321 tcaatggatc agattaacga cacgctggtt tcttccatca aaatcattgc gatgatgctg
     7381 ttgatcatcg gtggtggcgg tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa
     7441 tacattgctt ccatgatgca cgaaaccaac atttctccgc tgctgatggc ctggtcgatt
     7501 gctgccgtac tgcgtatcgc gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt
     7561 atcgcggcac cgctgattgc aacgacgggt gttagcccgg agctgatggt tattgcggtg
     7621 ggttccggta gtgtgatttt ctctcatgtg aacgatccgg gcttctggct gttcaaagag
     7681 tactttaacc tgactatcgg cgagaccatc aaatcctggt cgatgctgga aaccattatc
     7741 tcggtgtgcg gtctggtagg ctgtctgctg ctgaatatgg tgatttgata acactgactg
     7801 ccggatgcat cgcatccggc atcaaccgca ctctactgct tcttcgctgc agctctgcgc
     7861 cgtctgtcca aatccttcag caacttattc acgccatcat cggcaaacat cgtctcaagc
     7921 gttgcggaaa gcttgcgtcg ccagttttta tactggtaac tggtgccagg aatattcacc
     7981 ggttcggcca tatccagcca gtcttccggc tgtagtccta acagagcact gttactgtcg
     8041 gcaatgtagc gctgcaaacc acgattaagc gtcggcgtca tcgacatcaa cgatgcctta
     8101 tgcccggcac gttttggcag acaaccatat ttatgcagtg catccagcag cccttgcttc
     8161 gccagttcgc gatcctgata cagaccgcgc agtaccactt catccggata cagccccagg
     8221 gttttgccca acgttagatc cccgctctcc cagtaaccgc gcagcgtagg caggtcatgt
     8281 gtcgccgcaa ccgccatcga ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg
     8341 tggtcgtttt cgaaatagag cactttgtag gagtacacac cgctgctgcg cagcttaccg
     8401 acaatctcta ccggtacggt accgagatct tcaccaatca ccatacagcg atgacgttta
     8461 ctttcgagtg ccagaatcga gagcagatca tccaccggat agtgaacata cgcgccctga
     8521 tctgccgtct cgccatacgg tatccaccac aaacgcagca tcgacatcac atggtcaatt
     8581 cgtaatgcgc cgcagttttg catattggca cgcaacagct cgataaacgg ttcataggca
     8641 cgcgcggtga tgatatgcgg gtccattggc ggtaatcccc agttctgccc caacgggccg
     8701 aggatatccg gcggcgcgcc aaccgacgct ttcaggcaat atagttcacg gtcacaccag
     8761 gtttccgccc caccttccgc tacgccaacc gccagatcac gatacaagcc aatcggcatt
     8821 tcatagccct ggcttatctc ccagcaggcg gcaaactggc tgtaagccag ccactgcaac
     8881 cagagataaa aatcgacgtc atcacgatgt tcttcgcaga actgacgcac ttctggtgaa
     8941 tccacgttct gatacatctc tggccatgca ggccagcccc agcgcatttc gtcctctttc
     9001 acttgctggg catgtagcgc atcaaaggct gcctgccaga acaggctgtc gccctgctct
     9061 gcaacaaact ggcgaaacgc ggtcatctgc tcatcatcac gttgcgcgaa acctttccac
     9121 gccattcgta atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca
     9181 tcgcgcgcct gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct
     9241 tcgctaagat ggaaatcttc aacggcgtta acgtcgatat aaatcacatt cagccaacgg
     9301 cgagaagacg gactgtatgg gctggcactc tccggatttg ccggatagag cgcatgaatc
     9361 gggttcaggc cgatgaacga cccgccacgt tttgccacat ccaccagcat cgctttgaga
     9421 tcgccaaaat ccccaatacc ccagtttttt tccgatcgca gcgtataaag ctgaacgcag
     9481 gcaccccaca gcttttgttt attcagcaac gcctgcggtt cgtaacagcg tttcggggcg
     9541 acaatcaccc ggcaatgcgc gcgctggtcg tcctgggtga gtgtcagcgt gtgataacct
     9601 tccggcagct tcgtcggtag attgaacgct ttgccccccg ttacatggcc tttgtactgt
     9661 gttccttctt cggtggtcag cagccagcta tattcgccgc tgccctccac caccatcggc
     9721 atttttttgc cgctggtata aaccatgaca ttcgggactg gcgttaccgc cactttcgtg
     9781 gcggtacgtt gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc
     9841 ggtttaccgt gggcattgat gtaattgggg ctaatccccg ccgccagcgc ggcattatcc
     9901 agacgtttgc tttccatcga gcttccttag cgttttgcct gccagatacg agcctgataa
     9961 tcgcgaatag agcgatccga gctaaacata ccgcagcggg cggtattgag gatcgccgcg
    10021 cgagtccagg cctcctggtc gcggtacagc acatccacct gcttttgtgc ctcaacatag
    10081 gctgcgaaat ccgccatcac cagatacgga tcgccgcctt gtttgccgat actgtgcagc
    10141 atctggtcga aggcatgctt atcgccgtcg ctgtatttac cgctttccag ctctttcaat
    10201 actgcgtcca gcaccttatc tttcttccgc catttcaccg ggtcgtagcc tttggccaga
    10261 attgccttca cttgttccac ggtatgacca aaaataaaga tattttcttc accgactttc
    10321 tcggagattt caacgttcgc accatccagc gtaccgacag taagcgcacc attgagcgcc
    10381 agtttcatat tgccggtacc ggaagcttct ttacctgcag tcgaaatttg ttcggagata
    10441 tccgccgccg ggatcagttt ttccgccgcc gaaacgcaat aatcctgcag gaacaccacc
    10501 ttcaacttat cgccaaccag tggatcgttg ttgatcacgt cagccacttt gttgatcgca
    10561 aagataatat tcttcgccag gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg
    10621 cgcggtacgc gatcagcctg cgggttttca cgaatttctt tgtacaacgc cagaatatgc
    10681 agcagattca ggtgctggcg tttgtactca tgcagacgtt taatctgaat atcgaaaatc
    10741 gcctgtggat tgatctcaat accggtacga actttcacaa actccgccag acggacttta
    10801 ttcgcctgct tgatctcgcg atattgctga cggaatttcg catcatcagc gaatttttcc
    10861 agattgatca gctgatcgag atcgttagcc cactcttttt gcagtaattt atccaacaga
    10921 gccgccagtg cagggttgca ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg
    10981 ttatggaatt tgttcggcca tagctggtga tattccggga acagatcttt caccaccaga
    11041 tccgagtgca gcgccgcaac accgttcacc gcgaaaccgc caaccacaca caggttcgcc
    11101 atatgcactt gtttatcgtg caccaccgcc agtttggccc acactttttc atcgcccggc
    11161 caggttttct ccaccagcgt tttaaagcga gtattaattt cgttaataat ctgcatatgg
    11221 cgcggcagta agcctttcac cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg
    11281 gtatggttgg tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc ccagctcatc
    11341 tggtgctcat cgatcagcac gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg
    11401 ttcagctgaa taacttcata atccgccagt tcgtgcagtt tacgccccgc cagatgatgg
    11461 cgacgcaaaa tatccgctac cgaacaggca cactggaagt actgctgcat caagcgcagc
    11521 tttttaccgg cagtatggtt gtcgtttgga tagagaactt tggtcagttt ttccgcattg
    11581 atgccctgct gttcggcacg caggaaatca ccgtcgttaa atttagtcag atcaaacgga
    11641 tgcgcgtgcg tcgcctgcca cagacgcagc ggctgcgcca cgccattacg atagccgaca
    11701 acggggagat cccacgcttg accggtaatg gtaaactccg gctcccagcg tccgtctttc
    11761 gtcactttac cgccaatccc tacctgcaca tccagtgctt cgttgtggcg gaaccacggg
    11821 tagttactac gatgccagtc atccggcgct tcaacctgtt tgccatcgac aaaagactgg
    11881 cggaacaaac catattgata gttcagaccg taacccgtcg cagactgacc gatagttgcc
    11941 attgagtcga ggaagcacgc cgccagacgt cccagaccac cgttacccag cgccgggtcg
    12001 atctcttctt ccagcaggtc cgtcagattg atgtcataag ccttcaacga atcctgtact
    12061 tcctgatacc agccgagatt caacaggttg ttgcccgtca ggcgaccaat caaaaactcc
    12121 attgagatgt agttaacatg tcgctgattc gccaccggct tggcgaatgg ctgagcacgc
    12181 agcatttcgg ccagtgcttc actcactgct agccaccact ggcgaggagt catttcagcc
    12241 gcagaattta agccataacg ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg
    12301 ttaaaaatag gttgtgacat aggagttcca cttttcttag attttcaaca caacgttatc
    12361 gctagtttgc caggctcgat gttgaccttc ctcatcctgc gggggattag gcagggagga
    12421 gttgcgggga tgagcaagga aatgtgatct caaccactta aagctagtgc aaaccacagg
    12481 attagcagca aatcaatgca atacagcgca gaaaatctgt atctaagtgc aaaaaatggc
    12541 cgttgcgtat tttcaaaaag cggaaggtaa ctctataaat taagtaaagg agtgaaacag
    12601 tttcacaagt aaaatatcca gtgtgctcca tctcattctt aatagattta ttaagatcat
    12661 ctttttagat ggcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat
    12721 gaacataagg gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata
    12781 aagatttgga attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca
    12841 ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat
    12901 ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc
    12961 ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa
    13021 caacttccgg ctggcgctga tcacgagtcc tgcgggctac ggaaagacca ccctcatttc
    13081 ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca
    13141 gcaagagcgt ttcgccagct atctcattgc caccgtgcag caggcaacca acggtcactg
    13201 tgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc
    13261 ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta
    13321 tcatctgatc actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc
    13381 agaaaatctc accctggtgg tgttgtcacg caaccttccg caactgggca ttgccaatct
    13441 gcgtgttcgt gaccaactgc tggaaattgg cagtcagcaa ctggcattta cccatcagga
    13501 agcgaagcag ttttttgatt gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg
    13561 gatttgcgat gacgtttccg gttgggcgac ggcactacag ctaatcgccc tctccgcccg
    13621 gcagaatacc cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca
    13681 tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt
    13741 tctgttgaaa agcgccattt tgcgctcaat gaacgatgcg ctcatcaccc gtgtgaccgg
    13801 cgaagaaaac gggcaaatgc gtctcgaaga gattgagcgt caggggctgt ttttacagcg
    13861 gatggatgat accggcgagt ggttctgcta tcacccgctg tttggcaact tcctgcgcca
    13921 gcgctgccag tgggaactgg cggcggagct gccagtggga actggcggcg gagctgccgg
    13981 aaatccaccg tgccgccgca gaaagctgga tggcccaggg atttcccagc gaagcgattc
    14041 atcatgcgct ggcggcaggc gatgcgctga tgctgcgcga tattctgctt aatcacgcct
    14101 ggagtttgtt caaccatagc gaactgtcgc tgctggaaga gtcgcttaag gccctgccgt
    14161 gggacagttt gctggaaaat ccgcagttag tgttattgca ggcgtggcta atgcaaagcc
    14221 aacatcgcta tggcgaagtt aacaccctgc tagcccgtgc tgaacatgaa atcaaggaca
    14281 tcagagaagg caccatgcac gcagaattta acgctctgcg cgcccaggtg gcgattaacg
    14341 atggtaatcc ggatgaagcg gaacggctgg caaaactggc actggaagag ctgccaccgg
    14401 gctggttcta tagccgcatt gtggcaacct cggtgctggg tgaagtgctg cactgcaaag
    14461 gcgaattgac ccgctcactg gcgctaatgc agcaaaccga acagatggca cgccagcacg
    14521 acgtctggca ctacgcctta tggagtttaa tccagcaaag tgaaattctg tttgcccaag
    14581 ggttcctgca aaccgcgtgg gaaacacagg aaaaagcgtt ccagctgatc aacgagcagc
    14641 atctggaaca gctgccgatg catgagtttc tggtgcgcat ccgagcacaa ctgttatggg
    14701 cctgggcacg gctggatgaa gccgaagcgt cggcgcgtag cgggattgaa gtcttgtcgt
    14761 cttatcagcc acagcaacag cttcagtgcc tggcaatgtt gattcaatgc tcgctggccc
    14821 gtggtgattt agataacgcc cgtagccagc tgaaccgtct ggaaaacctg ctggggaatg
    14881 gcaaatatca cagcgactgg atctctaacg ccaacaaagt ccgggtgatt tactggcaaa
    14941 tgaccggcga taaagccgcc gctgccaact ggttgcgtca tacggctaaa ccggagtttg
    15001 cgaacaacca cttcctgcaa ggtcaatggc gcaacattgc ccgcgcacaa atcttgctgg
    15061 gcgagtttga acctgcggaa attgttctcg aagaactcaa tgaaaatgcc cgcagtctgc
    15121 ggttgatgag cgatctcaac cgtaacctgt tgctgcttaa tcaactgtac tggcaggccg
    15181 gacgtaaaag tgacgcccag cgcgtgttgc tggacgcatt aaaactggcg aatcgcaccg
    15241 gatttatcag tcattttgtc atcgaaggcg aagcgatggc gcaacaactg cgccagctga
    15301 ttcaactcaa tacgctgccg gaactggaac agcatcgcgc gcagcgtatt ctgcgagaaa
    15361 tcaatcaaca tcatcggcat aaattcgccc atttcgatga gaatttcgtt gaacgtctgc
    15421 taaatcatcc ggaagtgcca gaactgatcc gcaccagccc gttgacccaa cgtgaatggc
    15481 aggtcctggg gctgatctac tccggttaca gcaatgagca aattgcgggc gagctggaag
    15541 tggcggcaac caccatcaag acgcatatcc gcaatctgta ccaaaaactc ggcgtcgccc
    15601 accgtcaggc cgcggtacaa cacgcgcaga aactgctgaa gatgatggga tatggggtgt
    15661 gagtttagcc ggataatgca ttggatccgg tttacatctt tacatcattc aatactcacc
    15721 cgcgttacgc catctgtttc tatcaaacca aaccgcaccg gcaagaaacg ctccaccacg
    15781 gcgatattgg tcagcagatg gcatgaagga tgggcgaccg taaattctcc cgcgcccgcc
    15841 agcgccatcg gtagcaccag ctggtcggcg agatattccc ccactgccgc cgggcttgcc
    15901 aggtagcgtt tcacctcttt cactaactgc gcggctacca cctcggcact gacgcgcttt
    15961 tcaccgacga cgaaaaagcg ttcagtgata ttttcacttt cgacttccag cgagacggta
    16021 ttacccggcc cctgatcgcg cggcaggtta tgaatattct gttcatgcag ggaaaaactc
    16081 cccgccagtg tagcgatttc acgctcagca acatggcgcg gcacaccagc taatagaact
    16141 tctccacgca tctgcacaat gttcccgcgc tcgccaagtt gcaaggtgtt aaacgatgcc
    16201 acgggcgaga cttccgttgc caccacgccg cctccggcag gataaaaacc gtgacgtaac
    16261 agcgtggttt gctgatgaat tcctattttc gccagcagcg gctccagcac ccggcggata
    16321 aaatccgcag gcggcgccga cgggttatcg gtgcctccgc tcacttcaac acgcgaaggt
    16381 ccatcggcaa accacagcgc gggcagcacc gtttgcagca ccagcgtaca gcttccggca
    16441 ctaccgatag caaagcggta ctcgccgccg cgcacggcgc caggccggaa gaccagacgc
    16501 tgcgacccca gctccgcgcc ttccaccgtt gccctacaaa tttccgcagc cgcttttacc
    16561 gcggtcagat gctggcgcaa cagccccggt ttcgcccgcc cggcacgaat gctggtgatg
    16621 gtaaatggct ggccggttat catcgacagg ctcagcgccg agcgcagaat ctgcccgcca
    16681 ccttcgccct gtgcgccatc cagcgcaatc atccttttca tcatttatcc ttttacgcac
    16741 accacctgac gcagggtata gataacttcc accagatcgc tttgtgccgt catcaccgca
    16801 tcaatatctt tatacgccat cgggatttcg tcgatcactt cggcatcttt acggcattcc
    16861 acatgcgcgg tggcacgaat ttgatcttcc acgctgaaca gttttttcgc tttagttcgg
    16921 ctcattaccc gcccggcacc gtggctgcac gaacagaacg actcttcatt tcccagccca
    16981 cggacgataa agctttttgc tcccatcgaa ccgggaataa ttccatattg accagcacgc
    17041 gcagacaccg cgcctttacg cgtcacgtag atctcttcac caaagtgctg ttctttttgc
    17101 acatagttgt ggtgacagtt gatctcttcc atcgccagcg tttgtggctg tctgaccgtt
    17161 ttctgcgtaa tgctctgcaa tgccgttacc acgttttcca tcatcacatc gcggttaagg
    17221 ctggcaaaaa gctgcgccca ggccacggct ttcaggtaat catcaaagta ttccgtacct
    17281 tccataaagt acgccagatc acgcgacggc aacgtctcaa gcgtttcctg catctctttt
    17341 tgtgccagat cgataaagta agtcccgatg gcgttaccaa ttccgcgtga accggagtgc
    17401 agcataatcc acacctggtc cgactcatca aggcagattt taataaagtg gttaccggtt
    17461 cccagcgttc ccaggtgttt atagttattg gtattcagga aacggggata tttttgcgtt
    17521 aaccactgat aaccggcttc aagctcagcc catttcgcat cgacgttgac aggcgggttt
    17581 tcccatgcgc ctttatcacg tttacaacgc ccggtagtac gcccgtgcgg cacggccgtt
    17641 tcaatcgcct gacgcagctc tgccaggttt tcaggcaggt cttccgccgt taacgcggta
    17701 cgcagcgcgt tcattccaca gccaatatcc acgcccaccg ccgccggaat aatcgcccct
    17761 ttggtcggga tcacgctacc aatggtggaa tctttaccca ggtgtacatc aggcattacc
    17821 gcaatatgtt tgaaaataaa cggcatcttc gccgtattaa taagttgctg acgcgcatcg
    17881 gcttctaccg gcacgccttt ggtccacatt tttaccgggg cattttcagt ggtcagtaat
    17941 tcgtaattca ttttgttttt ctcttttcgt tgtttgctgt cctgataatt gcaaccgtcg
    18001 tgccagaaaa ttaaataagt aaattaattt tttaattcat tgtttttaaa aagattatat
    18061 ctttatgtcc gtaaccggag atttcccgca aagccgattt accgataatg aaatatcgtc
    18121 ttttataagg atatctaaga tgcgtaaaac agtagctttt ggctttgtcg gaacggtgct
    18181 ggattatgcc gggcgcggca gtcagcgctg gtcaaaatgg catccgacac tctgtttatg
    18241 ccagcaagaa tcgttggtca tcgatcgact ggaattattg catgacgccc gctcgtgctc
    18301 gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag aagtggtgag
    18361 cattgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct gcctgcatga
    18421 tttcgcccgt ggttacaagt ttcagccaga aaaagaagac tatttaattc acatcaccac
    18481 cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt atctacccgc
    18541 ccggttgata caaacttcac caccgcgcaa aaaagaacaa ccgcgcggtg ccggtgaagt
    18601 gacgattatc gatctcgatc taagccgtta taacgccatc gccagccgct ttgccgagga
    18661 acgccagcaa acgcttgatt ttcttaagtc cggcattgcc acgcgtaacc cccacttcaa
    18721 ccgcatgatt gagcagattg aaaaagtggc gattaaatcc cgcgcaccga tcctgcttaa
    18781 cggcccaacc ggtgcgggaa agtcatttct ggcgcgacgt atatttgaat taaaacaggc
    18841 gcggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc gcggcgatac
    18901 cgccatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc gggaatctcg
    18961 tgaaggttta ttacgcagcg ccaacggcgg aatgttgttt tttgatgaga ttggcgaact
    19021 gggcgcagac gaacaggcaa tgctgctaaa agccattgaa gagaaaacct tttacccgtt
    19081 tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg tgcgcgattt
    19141 gcgccagctg gttgccgaag gcaaatttcg cgaagatctg tacgcgcgga tcaatctctg
    19201 gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga accttgatta
    19261 tgaagtggag cgccacgcca cactcactgg cgacagcgtg cgttttaaca ccgaagcgcg
    19321 gcgcgcctgg ttggcttttg cgacctctcc ccaggcaaca tggcgcggta actttcgcga
    19381 actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca tcactctgga
    19441 cgtggttgaa gatgagataa accgtctgcg ctataactgg caggagagcc gcccctccgc
    19501 gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc gtatgcaact
    19561 ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgcagccg gacgacagct
    19621 ttttgatgtt tcgcgccagg gaaaagccag cgtcaatgac gcggatcggc tacgcaaata
    19681 cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct ccagttgaat
    19741 atgatggtcc gtcagcacct gcattacgct tactggcggc ggggcgtcgg tgtagacggc
    19801 atctaccatg ctgatgctgc ccatattgac catcgcgtta cggccaaatt tcgagtgatc
    19861 gacaaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtgc gaacttcgtg
    19921 gtaatcgaac tccagcagcg agccgtcgct atcgatgccg cttatcccca gaatgccgaa
    19981 atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc cgccatcgcg
    20041 gctgcgtaat tcgccaccag cgagaataat gcggaaatct tcttttacca tcaacgtgtt
    20101 agcaacattg agattgttgg tgacaatacg caaattgctg tgattaagca gcgcgtgcgc
    20161 caccgcttcc ggcgtggtgc cgatatcgat aaacagtgtc gagccgttgg ggatttgctc
    20221 cgccactttg cgggcaatcc tctctttttc ttcggtctgg gtggctttgc gatcgtgcca
    20281 cggcgtgtta accgaactgg aaggcagcgc cgcaccgcca tggtgacgca ggatcaggtt
    20341 ttgctccgcc agctcattga ggtcgcggcg aatagtctgc gggctgacgg agaaatgctc
    20401 taccagctct tcagtactga cataaccctg ctgtttaacc agttcgataa taccgttgtg
    20461 acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc gcattgagcg
    20521 aatcaacaaa agccatcgct aaacccacgg ctaacccggc gatgtgtgct ccgttcgcca
    20581 tcgacatccc aaacaaatca aaccatccgg cgataatcca gatcagcgca aagataatta
    20641 acccacgttg caggtaaatg ccactttgcg gatcgcgctc accacgtagc cagacgtagc
    20701 ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg aatttttgct
    20761 gcacatagcc gcttaacagg gcactaatga gagtaataac aattagctta ccgctaccga
    20821 ggcgtttttc caccgcaccg ccgagatacc accaccagag caggttaaag aggatatgca
    20881 tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat ttcagtgttg
    20941 gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt tgcatggcga
    21001 taaacaccac cacgcaggcg atcatcacca cccaggttac cggacctgcg cgttcacgca
    21061 aggcagcaaa gaaaggataa cggcgataat gcagaccact gccggtatgg cccgcctgcc
    21121 agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc agctccgccc
    21181 gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta tgttgttgaa
    21241 tcgtaaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc acgcgggggt
    21301 tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa aaggggacag
    21361 tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgacgctgcc aggcttcaaa
    21421 accgccgtca atgctataga ccacatcgta gccctgttgc agcagatact gcgccgcgcc
    21481 tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt cgttatcacg
    21541 cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat gtcccattgc
    21601 gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca acttctggtg
    21661 cgcgtcggca acgttaatac attcgaacag atccatgcgt ctctctttct ttacaaacaa
    21721 gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat aatgttatac
    21781 atatcactct aaaatgtttt ttcaatgtta cctaaagcgc gattctttgc taatatgttc
    21841 gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga aaccaaagat
    21901 ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc cgctggacgc
    21961 ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc ttccgccagt
    22021 tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg cctggtcagc
    22081 gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc cttcccaatg
    22141 cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg gattggtctg
    22201 tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaaccgg tttgcgtttt
    22261 ggcgcaaact cagtgttaaa accggaaatt aagcgcggat tcgaatattc tgactgttgg
    22321 gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa aggcggcgaa
    22381 gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg gattgtggaa
    22441 gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct ggttaacgcc
    22501 accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc gccttatggc
    22561 attcgcctga tcaaaggcag ccatattgtg gtgccgcgtg tgcataccca gaagcaggcc
    22621 tacattctgc aaaacgaaga taaacgtatt gtgttcgtga tcccgtggat ggacgaattt
    22681 tccatcatcg gcaccaccga cgtagagtat aaaggcgatc cgaaagcggt gaagattgaa
    22741 gagagtgaaa tcaactatct gctgaaagtg tataacgcgc actttaaaaa acagcttagc
    22801 cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga tgagtccgac
    22861 tcgccgcagg ctattacccg tgattacacc cttgatatcc atgatgaaaa cggcaaagca
    22921 ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc agaacatgcg
    22981 ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa agagagcgtg
    23041 ctgccgggtg gtgccattga aggcgaccgc gacgattacg cagctcgtct gcgccgtcgc
    23101 tatccattcc tgactgaatc gctggcgcgt cattacgcac gcacttacgg cagcaacagc
    23161 gagctgctgc tcggcaatgc gggggcgata agcgacctcg gggaggattt cggtcatgag
    23221 ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg caccgacgat
    23281 gccctttggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca atctcgtgtg
    23341 agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta aggatgatgc
    23401 ccggttgaag tgcaaccggg ctatttggat tccttagact attcattgta aataattgaa
    23461 taacggcccc tgagctaaaa tcgccaaact taagcacgtt acccaatcgc cccacttctt
    23521 cctctggttt ctattttttc tgcaccaatg gcaataatat cccaatgcaa aaccaagctg
    23581 tgcactaatc gggccgacaa aatcagtcca aggctgcggc caaaaagggg aaaaattcag
    23641 gggaggccag acaaacgtca gaaagaaatt tcggatattt cccagcaacg ctgatttctc
    23701 tggagaaata taaagaa
//
LOCUS       DAJTYD010000049        22830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTYD010000049 DAJTYD010000000
VERSION     DAJTYD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.049"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(150..464)
                     /gene="pspE"
                     /locus_tag="OO585_001741"
     CDS             complement(150..464)
                     /gene="pspE"
                     /locus_tag="OO585_001741"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6629363.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(539..760)
                     /gene="pspD"
                     /locus_tag="OO585_001742"
     CDS             complement(539..760)
                     /gene="pspD"
                     /locus_tag="OO585_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6629364.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(769..1128)
                     /gene="pspC"
                     /locus_tag="OO585_001743"
     CDS             complement(769..1128)
                     /gene="pspC"
                     /locus_tag="OO585_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6629365.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1128..1352)
                     /gene="pspB"
                     /locus_tag="OO585_001744"
     CDS             complement(1128..1352)
                     /gene="pspB"
                     /locus_tag="OO585_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6629366.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1406..2074)
                     /gene="pspA"
                     /locus_tag="OO585_001745"
     CDS             complement(1406..2074)
                     /gene="pspA"
                     /locus_tag="OO585_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6629367.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2241..3218
                     /gene="pspF"
                     /locus_tag="OO585_001746"
     CDS             2241..3218
                     /gene="pspF"
                     /locus_tag="OO585_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6629368.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFATQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3338..4603)
                     /gene="puuE"
                     /locus_tag="OO585_001747"
     CDS             complement(3338..4603)
                     /gene="puuE"
                     /locus_tag="OO585_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6629369.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4641..5910)
                     /gene="puuB"
                     /locus_tag="OO585_001748"
                     /pseudo
     CDS             complement(4641..5910)
                     /gene="puuB"
                     /locus_tag="OO585_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(5912..7399)
                     /gene="puuC"
                     /locus_tag="OO585_001749"
     CDS             complement(5912..7399)
                     /gene="puuC"
                     /locus_tag="OO585_001749"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6629370.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7674..8231)
                     /gene="puuR"
                     /locus_tag="OO585_001750"
     CDS             complement(7674..8231)
                     /gene="puuR"
                     /locus_tag="OO585_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6629371.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8258..9021)
                     /gene="puuD"
                     /locus_tag="OO585_001751"
                     /pseudo
     CDS             complement(8258..9021)
                     /gene="puuD"
                     /locus_tag="OO585_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9233..10651
                     /gene="puuA"
                     /locus_tag="OO585_001752"
     CDS             9233..10651
                     /gene="puuA"
                     /locus_tag="OO585_001752"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6629372.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10800..10965
                     /locus_tag="OO585_001753"
                     /pseudo
     CDS             10800..10965
                     /locus_tag="OO585_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10955..12340
                     /gene="puuP"
                     /locus_tag="OO585_001754"
     CDS             10955..12340
                     /gene="puuP"
                     /locus_tag="OO585_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6629373.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12474..12719
                     /locus_tag="OO585_001755"
     CDS             12474..12719
                     /locus_tag="OO585_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6629374.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13032..14675
                     /gene="sapA"
                     /locus_tag="OO585_001756"
     CDS             13032..14675
                     /gene="sapA"
                     /locus_tag="OO585_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6629375.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLKFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14672..15637
                     /gene="sapB"
                     /locus_tag="OO585_001757"
     CDS             14672..15637
                     /gene="sapB"
                     /locus_tag="OO585_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6629376.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15624..16514
                     /gene="sapC"
                     /locus_tag="OO585_001758"
     CDS             15624..16514
                     /gene="sapC"
                     /locus_tag="OO585_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6629377.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMLAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16514..17506
                     /gene="sapD"
                     /locus_tag="OO585_001759"
     CDS             16514..17506
                     /gene="sapD"
                     /locus_tag="OO585_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6629378.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17508..18314
                     /gene="sapF"
                     /locus_tag="OO585_001760"
     CDS             17508..18314
                     /gene="sapF"
                     /locus_tag="OO585_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6629379.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18382..18735
                     /locus_tag="OO585_001761"
     CDS             18382..18735
                     /locus_tag="OO585_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6629380.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19103..19891
                     /gene="fabI"
                     /locus_tag="OO585_001762"
     CDS             19103..19891
                     /gene="fabI"
                     /locus_tag="OO585_001762"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6629381.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20036..21163
                     /locus_tag="OO585_001763"
     CDS             20036..21163
                     /locus_tag="OO585_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6629382.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21231..>22830
                     /gene="rnb"
                     /locus_tag="OO585_001764"
     CDS             21231..>22830
                     /gene="rnb"
                     /locus_tag="OO585_001764"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6629383.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAE"
BASE COUNT         5142 a         6053 c         5964 g         5671 t
ORIGIN      
        1 aattgagcac aagggtttta gtcacgcagg aaatcatttt ttaccaggga aaaagcgtat
       61 ttgtgagctg acgcaaataa tctatgggga tgtaaataag gtgtgacaga aaaaaaacgg
      121 cgcataagcg ccgctcatgg tgaattcttt taacctttga ccttcggcat tgcgatgtct
      181 ttcaagccac cggcgttctc aacgtgggta tatcccatct cgctaaggat ctcttttgct
      241 tgccctgact ggcgtccggc attgcaatac actttcacgg tgtcgttttt atccggaacg
      301 gcggtggcaa tgcgctcttt cacttccttc aggggaatat tgatggcccc ctgaacgtgc
      361 tcttgctgat actgctctgg aacacgaaca tcgatccagt gttcagcggc gaaaacgggc
      421 agtgaaaaca ccagtgccag agcaagtaag ccttttttaa acatgtaagc gtccttctat
      481 ggatgaaaat tgccatccat tatacgcctg agatttaaca atttttgcga aactcccctc
      541 accttttata acgctgtgcc agtttattag cagcccgact taacagcggt tccagcgcca
      601 cagccagcaa cattttcagc ggtcggcgag caactgattt tatcgcccag cccgctacac
      661 ccgccgggcc atagcgcagt gcggtaagaa gtaccagctt gcctgctaat ttgaaaccag
      721 gctttacctt ttgcccggcc tgttgccagc gagtattcat aactttcctc acagttgacg
      781 gaaacggcta cgtaacgtga aagtatcgga agtgacataa cgttccatct cgcgtaaacg
      841 cgtttcactt gccgccagtt cacggtcgac ttcatccagc aattcgctgc tggagggtag
      901 ctgctcacca aaggccatgt tgtccggcat tggatcaagc gcaaatgaca aaatgatgta
      961 agcaaccagg gtaaacagcg ccagaccgaa gaaaatcgac agcaccacca ggatacgcac
     1021 cagttttacc ggtacatcaa aatagttggc aatcccggcg cagacgccgc ggaccatgcc
     1081 ctgctgtgga atacgccata attttttatt gagattaatg cccgccatta gcgatccctc
     1141 cagttcggat gttctgcatc aagaatagat tccagcgcct gaatacgttc gcgcatccgt
     1201 tttgcttcat cagccagttg cgctaatcgc tgctgctcac tttgcgacaa ttcactgcga
     1261 ccagaacgat tgctgtaatg cagccataac cagatcggta aaacaaacag cacaaaaatg
     1321 gttaacggaa tagccagaaa tagcgcgctc ataagtactc cttagacgat gagcggcggc
     1381 gcgttcggac gccgcctaga tgttattatt gattgtcttg cttcattttg gcttttaatt
     1441 gtgccagttg ttcgctgatt gcatcatcgg ctttcagttc ggcaaactga tcgtccagcg
     1501 atttttgttt accgaagctg tggctttctg cttccgcttc catctggtca atacgacgtt
     1561 cgaaagattc gaaacgagcc attgcttcat ccagtttgcc actgtccagc tgacgacgca
     1621 catcgcgcga cgagtttgcc gcctgatggc gtaacatcaa tgcctgctgg cgagcgcgtg
     1681 tttcgctcaa tttgttttcc agctcaccgc tctctttctt catgcgtgcc agcgtatcgt
     1741 ccaccagcgt cacttcatgt tccagactct taatcagatc ggtcagtttc tgtttttcaa
     1801 ttaacgccgc acgtgccaaa tcctctctct ctttcagcag cgccagttcg gctttttcct
     1861 gccattcaac ttcacgcgcc gacgcttgtt caatacggcg agtcagctgt ttcttttctg
     1921 ccaacgcacg cgccgaagta gaacgtactt caaccagtgt atcttccatc tcctggatca
     1981 tcagacgcac cagtttctgt ggatcttccg ctttctctaa cagagcgttg atgttggcat
     2041 tcacgatgtc ggcaaagcga gaaaaaatac ccataatgtt gtcctcttga tttctgcgtt
     2101 caggattgtc ctgctgaact gttaatacaa tttgcgtgcc aattttttat ctttttgatt
     2161 tataaagatc tgattgaaga atcatcagca acatgccagg atgaattagc gaattacact
     2221 aacaagtggc gaatttcatc atggcagaat acaaagataa tttacttggt gaggcgaaca
     2281 gctttctcga agtgctggaa caggtttcgc atctcgcacc gctggacaaa ccggtgctca
     2341 tcatcggcga acgcggcacc ggtaaagagc tgattgccag ccgcctgcat tatctctcct
     2401 cccgttggca agggccgttt atttccctta actgcgcggc gttaaatgaa aatctgctgg
     2461 attccgaact gtttggtcac gaagcggggg cgtttaccgg tgcgcaaaaa cgtcatccag
     2521 ggagatttga acgtgccgac ggcggtacgc tatttcttga tgaactcgct acggcaccga
     2581 tgatggtgca ggagaaatta ttgcgcgtga ttgagtacgg tgaactggag cgcgttggcg
     2641 gcagccagcc attgcaggtg aatgtgcggt tggtatgcgc gacgaatgcc gatctcccgg
     2701 cgacggtcaa tgaaggcact tttcgcgctg acctgctcga ccgactggct tttgatgttg
     2761 tacaactgcc accactgcgc gagcgcgaaa gcgacataat gttgatggca gaacactttg
     2821 ccacccagat gtgtcgggaa atcaagctgc ctctgttccc ggggtttacg gagcgcgcca
     2881 gagaaacatt gctgaattat cgttggccgg gaaatattcg tgaattgaaa aacgtggtgg
     2941 aacgttcagt gtatcgccac ggcaccagcg attatccgct tgatgacatc attattgatc
     3001 cctttaaacg gcgtccgcct gaagacgcta tcgccgtttc agaaaccacc tcgcttccaa
     3061 cactgccgct ggatttacgt gagtttcaga tgcagcagga aaaagagttg ctgcaactca
     3121 gtttgcaaca agggaaatat aaccagaagc gcgcggctga attactgggg ttaacttatc
     3181 atcagttccg cgcgttgttg aaaaagcacc agatttagcg aagttgagca atacaacttg
     3241 ccggatgcgg cgtgaatgct ttattcggca tacataatca ataagtcgtt tgcaggcctg
     3301 ataagcgcag cgcatcagac attattgcgt tgggctatta atcgctcagc gcatcctgca
     3361 aaattttcat tgccgcatcg aattgcgcat ccggaatggt cagcggatac aggaagcgaa
     3421 tcacgttgcc gtatgcgcca caggtcagca ggagcagccc ctgcgccagc gcgcgttgct
     3481 ggattttctg tgcaatcgcc gctgacggct cgcccgtttg cggatcgtta aactctgccg
     3541 caatcatcga ccccaggccg cgtaccgcag caatggccgg aacgctttct ttggcatcaa
     3601 tcaacgtgtt tttgagacgc tggcccagtt gattcgcgcg ttcgcagagt gattctttgt
     3661 cgataatatt gagcaccgcg tgcgcggcag ccaccgccag cgggttcccg gcgtaggtgc
     3721 cacccaaccc gcccggcgcg ggcgcgtcca taatattcgc gttaccgacc acgcccgaaa
     3781 gcggcatccc gcccgcgagg cttttcgcca tcgtcattaa gtccggctta tcggcgtaat
     3841 gatccatggc aaacagttta ccggtacgcg caaagccgct ttgcacttca tcggcaatca
     3901 tcacaatacc gtgctcgtcg cacaggcggc gaatggcggc aaccagctct tttggcgcaa
     3961 cgttgaaacc gccctcgccc tgcaccggtt cgaaaataat ggccgccacc tgcttcgctt
     4021 caatgtccga tttaaacaag cgttcgatgg cgtcgaggga gtcctgtgtt gaaatgccgt
     4081 gtaaatctga cggataaggt acgtgataca ccgaaccagg gaacgggccg aagccgattt
     4141 tgtacggcgc aacttttccg gtcagcgcca tggtcatata tgtacgaccg tggaagccgc
     4201 cgctaaacgc aatcacgcca ggtcgtccgg tatgggcgcg agcaattttc accgcgtttt
     4261 ccaccgcttc cgcgccggta gtgaagaatg cggttttagc ctgcccgctc actggggcaa
     4321 gggcgttaat tttctccgcc agcgtgacgt agctttcgta cggcacaatc tgatacgccg
     4381 tgtgggtaaa ctgttgcaat tgctgctcca ccgccgcgac cagatcaggg tggcgatgcc
     4441 cggtattcag caccgcaatg cctgcggcga aatcgatgta ttcgttgccc tctacgtcct
     4501 tcagcgtagc gttttctgcc gactgggcga agaagttaca catcacgcca accccgcgtg
     4561 gggtagcaga aagacgacgc tgatggaatt cattgttgct catgtttcac accttttgac
     4621 tcttcactgt taatcggaaa ttaaacgccc agacgatcgc gcaggctgta ataagccgcg
     4681 cccatcgcgg taaatggcac gcgcagcgtg cgcccgccgg ggaacgggta atgcggcaga
     4741 ttggcgaagg catcgaaacg ctcggcgtca ccgcgcagca gttcagcaat caaacgcccg
     4801 gcgaggtggg tacaggtgac gccgtggccg ctgtagccct gcatgtaata gatgttggta
     4861 tcgaggcgac cgaactgcgg catccgtgaa agggtcagca ggaagttgcc cgtccagcga
     4921 tagtcgattt tgacgccctt cagctgcggg aaggttttca gcagtttcgg cacaacaaga
     4981 cgctcaacgt catccgggtc gcgcgcgccg tagaccacgc cgccgccgta cagcaaacgg
     5041 ttgtcggcgg taagacggta gtaatcaagc agataattac agtcttccac acagtagttt
     5101 ttcgggatca gcgaacgggc taaatcttcc gacagccgtt cggtggtgat cacctgcgtg
     5161 ccgcacggca tgctgcgttt cgccagttcc ggctctactt tatcgcccag atacgcattc
     5221 ccggcgacaa tcacatactt cgccgtcacc tgacctttgg cagtgcgcac aacggctggc
     5281 gtggtgtgct ggatctgcgt cacggcagaa agttcataca cgcgcccgcc gttgaggcgg
     5341 atggcgtccg cttcgccaat ggcgaggttt agcgggtgaa tatgcccacc gctgtgatcc
     5401 agcagcgcac cggtatagcg atcgctggct acttcacggc gaatggcgtt ggcgtccagc
     5461 aattccagct gtttattgcc gtagcgttcc cagttctctt tctgctcttc aagtgtggcg
     5521 agctgtttat cattcatcgc cacaaacagg ctgccggggc ggtagtcgca gtcaatctga
     5581 taacgtttga tacgttcgcg gatgatctca ccaccttcga acatcatgct gccgagcata
     5641 ggggctgtgt ccatgccgta gcttttttcg atcacgtcga tgtcgcggtt gtaggagttc
     5701 acaagctgcc cgccattgcg cccgcttgcg ccaaagccga tgcgtgaggc ttcgagaacc
     5761 actacgtcaa agcccgcttc cgccagatgc agcgcggagg agagcccggt atagccgccg
     5821 ccaaccacgc aaacgtcgca ggtgattgac tcattcaggg tgtcgaatgg cacgtattta
     5881 tgcgtaataa ctgctggtat gttcggtcat ttcaggcctc caggcttatc cagatggttt
     5941 tcagttcggt gaatttttca agggcatgca gggatttgtc gcgaccgttg ccgctctgct
     6001 tatagccgcc aaacggcacg gtcatatcgc cgtcgttgta gttattgacg aagacggaac
     6061 cggctttcag gcggcggctc atacggtgcg cgcgggagag gtcgcgcatc cataccgccg
     6121 cgccaaggcc gtactggctg tcgttggcaa gctgtagcgc ctgttcttct gatgtgaaac
     6181 gcgtgaccac cagcaccgga ccgaaaatct cttcgcggct taaggacgca ttcgggtcta
     6241 catccacaaa gatggtcggg ccgatggcgg cagccagccc ggcgttacgg ccatccagca
     6301 gcagttgccc tttgccttcg ccctcacgaa taaagctatg gaccgagtcg gcgtgggcgc
     6361 agtcgattaa agtgcccatg gtggttgcgg ggtcaagcgg atggcccggc tgccagtttt
     6421 gcgcctgctg ttttaacagg gctaagaatt catcggcgat gctctcttcc agcaacaggc
     6481 gtgttccggc gatgcacacc tgaccctggt tgtagaagat gcctgctgcg gtggagctcg
     6541 ccgctttttg cagatccgaa caatcggaga aaacgatgtt ggcgcttttg ccgcccgctt
     6601 ccagccagac gcgtttcatg ttgctgtcgc ccgcatcttt cagcagctgt ttcccggtac
     6661 gggttgaacc ggtaaaggca atggcgtcga tatcgttatg acgcgacagc gcctgccccg
     6721 cttcatgacc aaaacccgtc accacgttca acacaccatc cggcaagcct gcttctttcg
     6781 ccagccccgc gagacgaatc gcactgagcg gtgatttttc agacggtttt agaatcacgc
     6841 tgtttcccgc cgccagcgcc gggccgagtt tccagcaagt cagcaacagc gggaagttcc
     6901 acggcacgat ggcggcaatc acgccgaccg gttcacgcac gatcatcgcc agctcatggc
     6961 tactggtggt cgccacttcg ccatacactt tgtcgatcgc ttcggcgtac cagcgaatgg
     7021 cgcgcgccgc gccgggaata tcatcacgca gactgtgacg aatcggtttg ccggtgtcga
     7081 gagtttccag cagtgccagc tcttcggcgt gggcttccat taaatcggcg agtttattca
     7141 gtaccgcttt acgtttagcc ggagaagaga gtgaccagtc gccgcgttca aatacgccgc
     7201 gtgctgcgct caccgcacgg tcgatatcga cgctcttgcc gcgggcaatt ttcgccagcg
     7261 gtgcctgggt gactggatca acggtttcaa aggtttcatt ttccgccgca gcagtatatt
     7321 caccgttaat aaataagcgg ttttcaatgg cgagacttaa cgctttatcc tgccagtaag
     7381 ccagatgatg aaaattcatt atgactcctg tttcacgtct atcagatata tgcagtttgt
     7441 gaacgtcagg gcgcagagat ccccgggccg tatgcccgtt tatttctggc gacattcttt
     7501 atatacactt catccttcaa gccgcctctg ccttggctgc gttcgctccc ccgaatcact
     7561 tacctgagta agctcatcgg gattcgctca cttgccgcct tgatgcaact tgaatgattt
     7621 tgtgtataga ttgccgggcg cattgcgctt acccggctta cagaacaaaa agattaaaac
     7681 gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt actgaaactg
     7741 tgcgggatgc cggtattaat ggcataactt tgacccgcga cgaggtggta atcctgacca
     7801 ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc ctgatgctta
     7861 attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga
     7921 ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat ctcaattaag
     7981 tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga aaagaattcc
     8041 gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc agggctgact
     8101 ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc ggcggcacga
     8161 cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg cgccagtccc
     8221 tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt ttttcagcga
     8281 tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaaga gcgtactcgc
     8341 tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg atgacgctaa
     8401 ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg ctaacgacct
     8461 tcgccccttg tccatgtaga gagtttaccc aaaagttgct acattcaggt aacaacgcag
     8521 acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc tgttccaccg
     8581 gcagttcgga tcttcccgat gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa
     8641 cgaaccaccg gttgccacca ccagttcttg taaaccccgg cagatggcga aaatggggat
     8701 gcgcctttcg agtgcggcat taattatcgc catgctcaga agatcacgcc cgggatcggc
     8761 gtcaggctca tcgccgtttt caccatatag gtgcggctgc acattgctgg gactaccagg
     8821 aagataaatg ccatcgagtt tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc
     8881 atgtggtagc gcaattggca agccgcctgc gtggatgatg gcattcaggt acttttcttg
     8941 cagagtctgg gtcgcatgac ccttaagcct gttcctgcac attacgacac cgataaccgg
     9001 attgttcatt atatttttca tgctcaatct cacaaagtgg actaaattat cgccattact
     9061 gcgttttaaa tgacctttat gttcaatatt ttttcaatct agcagtgggt tttcattttt
     9121 gcaaactaaa tttaacattt agcaaacatt tagtttgcat acagattcga atggtggtca
     9181 ttatatttta cgctttgata acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac
     9241 caatatcgtt gaagtagaga actttgttca gcagccagaa gagaggcggg gtagcgcctt
     9301 tacgcaggaa gtgaaacgct acctggagcg ctacccgaat acgcaatatg ttgatgtttt
     9361 acttactgat ttgaatggtt gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa
     9421 gttggagaaa gggtgttatt tcccggcctc ggtgtttgca atggatattc tgggcaacgt
     9481 agtagaagaa gcgggtctgg gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt
     9541 tctcggttcc ttaacccctt ctgccgccga tccagagttt atcggtcaga tgctcctgac
     9601 catggtcgat gaagatggcg ctccctttga cgttgagccg cggaacgttc ttaaccgcct
     9661 gtggcagcag ctacgccagc gcggattgtt cccggttgta gcggtagagc tggagttcta
     9721 tttactggat cgtcagcgcg acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac
     9781 cgataaccgc aatacacaaa gccaggttta ctccgttgat aacctcaacc acttcgccga
     9841 cgtgctcaat gatattgatg aactggcgca gttacagtta attcccgccg acggtgcggt
     9901 cgccgaagcc tcgccgggtc agtttgaaat caacctttac cataccgata acgtgctgga
     9961 agcctgcgat gatgcgctgg cgctaaaact cctggtgcgt ctgatggcag aaaagcataa
    10021 gatgcactcc acttttatgg cgaagccgta tgaagagcac gcgggcagcg ggatgcatat
    10081 ccatatcagt atgcaaaata atcgtggcga gaatgtgctt tctgacacag aaggcgaaga
    10141 ttcgccgctg ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc
    10201 gttgctggca ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt atgtgccgac
    10261 gcaggcgtcg tggggccata acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg
    10321 tcataatcac cgcgtggaat atcgcgtggc gggtgccgat gccaacccat atctggtgat
    10381 ggcagcgatt tttgccggta ttttgcatgg ccttgataac gagctgccgt tgcaggaaga
    10441 agtcgaaggc aacgggctgg aacaggaagg cttacccttc ccgattcgcc agagcgatgc
    10501 cctgggtgag tttatcgaga atgatcacct gcgccgctat ttaggcgaac ggttttgcca
    10561 tgtgtatcac gcctgtaaaa atgatgagct gttgcagttt gagcgcctca tcacagaaac
    10621 agaaattgag tggatgttga aaaacgcata gtaccccttg cggggttgtt tcatcgacct
    10681 gagcggagcc aggcctgaca tttgctggta atccagacat ttcccggatg tcgcgttatg
    10741 gagcgcaggc ggcaacgggc ttccgttaac gattaagact gtgacgagga aatgagatga
    10801 tgccgatgat taaatccccg catggcgagg gaggctgcgt atgcgcccct cctgctactg
    10861 actggactcc gtcccctttg cttcccttgc taaacaggtt tgatttcagg tcgacacgac
    10921 cgcaaacctt attacgcagg gggaggcagc aattatggct attaattcac cactgaatat
    10981 tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc aggtggtgat
    11041 gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta ttgtctccgg
    11101 cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg tgctgtttac
    11161 cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg cctataccta
    11221 cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc ggctggtcat cgctgctgga
    11281 ttatctcttt ttgccgatga tcaacgtctt gttggcgaaa atctatctct ccgccctctt
    11341 cccggaagtg ccgccgtggg tgtgggtagt aaccttcgtc gccattttaa ccgccgcgaa
    11401 tctgaagagc atcaacctgg tcgctaactt caataccctg tttgtactgg tgcaaatctc
    11461 catcatggtg gtgtttatct tcctggtagt tcagggactg cataaaggag aaggcgttgg
    11521 caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc cgattattac
    11581 cggggcgacg attgtctgtt tctccttcct cggtttcgat gcggtgacca cgctttcgga
    11641 agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg cggtctacgg
    11701 tggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg atatcagccg
    11761 ctttaaagat ccggatgccg cactgcctga aattgcgctg tacgtcggcg gcaagctctt
    11821 ccagtcgatt ttcctctgca ccacgtttgt gaacacgtta gcctctggcc tggcttcaca
    11881 tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc cggagcgcgt
    11941 gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta tggtcgggat
    12001 tgtcgcgttg tcggcgctgt tcttcgattt agttaccgcc acagcgttaa ttaacttcgg
    12061 tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct ggcggcgtaa
    12121 aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc tggttggcgc
    12181 gctgacggtg ggtgtgctgt gggttaacct cgagtcaacg tcactgacac tcggtctggt
    12241 atgggcttcg ttaggcggcg catatttatg gtatctgatc cgccgctatc gcaaagtgcc
    12301 gttgtatgat ggtgacagaa cgccggtgag tgaaacgtaa tagtcggatg cgcggcgcat
    12361 tatcctgccg gagggtaatg caccgcgcca ttaactataa aaagatgtat gacccggatc
    12421 acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa ataatgaacc
    12481 acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa tgcgcagagc
    12541 ctgtcgcaga cgctgaacgc acaccgctgg cccactactt ccagttactg ctcacccgtt
    12601 tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct gaagcgggga
    12661 ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt aatgagtaat
    12721 tatttaattg cagacgatcg gtcagtatcg ttgtccggat aaagcgttca cgccgcatcc
    12781 ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc gtaccgctct
    12841 gctcctcgct gagttgttct gtgagttata gttatagagg aagcacgggt acgaatgttg
    12901 aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta cgacccctga
    12961 acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt tacataaaat
    13021 cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga cttgtgagtg
    13081 gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac agcggttttg
    13141 tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc agtgggttaa
    13201 ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat ccctatacct
    13261 atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc gcgacctatc
    13321 gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact cccactcgta
    13381 aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc aacaacccgt
    13441 ggcataacgt caacggcagc aacttcccct acttcgacag cctgaaattt gccgataacg
    13501 tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc cagccggatg
    13561 cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca gaatatgccc
    13621 ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc ggcaccggtc
    13681 cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt catgatgact
    13741 tctggcgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc ggcggcaccg
    13801 gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct gctgccagcc
    13861 agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct gggatgaacg
    13921 tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct gtccgccatg
    13981 cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat ggtacggctg
    14041 aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag gctaaaatta
    14101 ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg gaaaacttaa
    14161 cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca ctgaaaactg
    14221 ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt gtgccggtag
    14281 aaggtcgctt tcaggaggca cggttgatgg atatgagcca tgatctgacg ttatccggtt
    14341 gggcgacgga cagtaacgac ccggacagtt tcttccgtcc tttactgagc tgcgcggcaa
    14401 ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc gtgttgcgta
    14461 aggcgctctc ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa gcgcagagta
    14521 ttctggcgca ggaattgccc attttgccgc tggcgtcgtc attgcgtttg caggcctatc
    14581 ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt gctggggtgt
    14641 atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac gccgcatttt
    14701 gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga gctatttcac
    14761 ccctcatgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct ggtttaacgg
    14821 cctgatccac tgggattttg gtgtttccag cattaacggt caaccgattg ccgaacagtt
    14881 aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt ttgcgctgat
    14941 tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc aggataactt
    15001 gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg cgcttctgtt
    15061 gacgctgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt tcgatctgct
    15121 ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt cggactcacc
    15181 atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg tgatcaccct
    15241 gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta tcgaagtgta
    15301 tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta ctattttgcg
    15361 tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct tacagttttc
    15421 caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg gtttagggcg
    15481 ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg gagtgatggt
    15541 gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg ccatggctaa
    15601 ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa aagcgcccgc
    15661 cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg atggtcggcc
    15721 tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt gcgccttacg
    15781 gcatcgacca gcaatttctc ggttatcaat tattgccgcc atcatggtcg cgctatggcg
    15841 aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc cgtttactga
    15901 gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg acgatctgtg
    15961 gcctagtact gggcaccttc gccggggcga cacacggcct ccgctcagcg gtgcttaacc
    16021 atattctgga taccttactg gcaatccctt cgctgctgtt ggcaattatc gttgtagcgt
    16081 ttgccggacc gagtttgtcc cacgctatgc ttgctgtctg gctggcattg ctgccgcgta
    16141 tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac gttatcgccg
    16201 cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg ccaaacatca
    16261 ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc gatatcgccg
    16321 cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga gcgatgctcg
    16381 gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca ggtgcggcaa
    16441 ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt gcgattattg
    16501 cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat ttaaaaccgg
    16561 tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag gtgaaatccg
    16621 cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga tttgtggggt
    16681 gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata tcgatttgct
    16741 gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga tgattttcca
    16801 ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga tgcaaaacat
    16861 cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca aacgccgtgc
    16921 cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc gcagttttcc
    16981 ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac tggcgaatca
    17041 accgcgtctg ctgattgctg acgaaccgac caactcaatg gagccaacaa cccaggcgca
    17101 aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt tgcttatcag
    17161 ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt actgcggtca
    17221 aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc cttataccca
    17281 ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa gtcgcctcaa
    17341 tacgctgccc ggcgctatcc cactgctgga acagttaccg attggttgtc gtctggggcc
    17401 acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg gggcgaaaaa
    17461 tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg atcgaaacgc
    17521 tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggctgg tttcgccgtc
    17581 agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag acactggcga
    17641 ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg ggaatgatag
    17701 agcctaccag cggcgagtta ttgattgacg atcatccact gcattttggc gattattcct
    17761 tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg aatccccgtc
    17821 aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccgacctg gaaccggaac
    17881 agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg gatcacgtca
    17941 gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg gcgcgcgcgc
    18001 tgatattgcg cccaaaagtc attattgccg atgaagcact cgcttcactg gatatgtcga
    18061 tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc atttcgtata
    18121 tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg ctggtgatgc
    18181 atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg ccgctgcatg
    18241 aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg gatgcgtggc
    18301 gtaaagatcg ctaattttct gaatcctcct tccagattat ccacctcatt ctgacaagag
    18361 attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg tgatttaaga
    18421 cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg acgttttggt
    18481 gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt tgcttgctgc
    18541 tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt tgcctatgat
    18601 accaggcgca ctagctggct tgagtcgcag ggctggaccg tgctgcgttt ctggaataac
    18661 gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact gaaccgccga
    18721 tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt gtggtgaaaa
    18781 catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc atcgccataa
    18841 aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg cacaatatgg
    18901 tcccctcgcc cctctgggga gagggttagt gtaaggggaa aagcgcccac cctgccgcag
    18961 cctgctccgg tcggacctag caactatagc tactcacagc caggttgatt ataataacca
    19021 tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg cacaacaagc
    19081 atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc tggtaaccgg
    19141 tgttgccagc aaactatcca tcgcctacgg tatcgctcag gcgatgcacc gcgaaggagc
    19201 tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag aatttgccgc
    19261 tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca gcatcgacac
    19321 catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac actctattgg
    19381 ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc gtgaaggctt
    19441 caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag cttgccgctc
    19501 catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg agcgcgctat
    19561 cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg tgcgctatat
    19621 ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg gtccgatccg
    19681 tactctagcg gcttccggta tcaaagactt ccgcaaaatg ctggctcatt gcgaagccgt
    19741 taccccgatt cgccgtaccg ttactattga agatgtgggt aactccgcgg cattcctgtg
    19801 ctccgatctc tctgccggta tctccggtga agtggtccac gttgacggcg gtttcagcat
    19861 cgctgcaatg aacgaactcg aattgaaata atcattctgt tggtaaatat tgggcggcgt
    19921 tctgccgccc gttatctctg ttataccttt ctgatatttg ttatcgccga tccgtctttc
    19981 tccccttccc gccttgcgtc aggataacga tttcctttac gaccaaggag cgcccatgga
    20041 acaacgccac atcaccggca aaagccactg gtatcatgaa acgcaatcca gtactgcgga
    20101 gtatgacgtt ctgcctctgg tcccggaagc cgcaaaggtc agcgatccct ttctgctcga
    20161 cgtgatcctt gatgaagaaa cgctagctcc cttcctttca tggctggtcc ctgcgcgcgt
    20221 tcttgcagtg gaattgttcc ctgaccagct taccgtgacc cgttcacaga ccttcaccgc
    20281 ttatgaacgc ttgtctacgg ccctgacggt tgctcaggtt tgcggcgtcc agcggttatg
    20341 taactactat tcggcgcgac ttacgccgct ccccgggcct gattcctcta gggaaagcaa
    20401 tcataggttg gcacaaatca cgcaatatgc ccgccaactg gctagctcgc cttctattat
    20461 cgacaaccga tcgcgccagc atctgaatga cgtcggtctt actgccaggg actgtgtaat
    20521 cattaaccaa atcattggtt ttattggctt tcaggcgcgg accattgcga catttcaggc
    20581 ttatctcggg cacccggtac gctggttacc cggtctggaa atacaaaact acgccgacgc
    20641 gtcactgttt gctgatgaat caatacgctg gcgaagcagc tatgaggtgg aaaaactacc
    20701 tgaagagtac acaaaaagtt caacagcaga actttgccaa ctggccgaaa cactctctct
    20761 ccaccctatt tcactttccc ttctcgaaag gttgttaaac agtacacggg ttaatacaca
    20821 gccggataat aagcttgcgg cgttgttatg cgcgcggata aatggcagtc ctgcttgttt
    20881 tgccgcctgt atggattcat caaatgaata taaaaaaatc agcccccttc tgcgcaaggg
    20941 cgaaaatgaa attaaccaat gggctgaccg tcattctgtt gagcacgcta ccgttcaggc
    21001 gatacaatgg ctgacccgag cacccgatcg ctttagcacc gcccagttca gccctttact
    21061 cgaacacgaa caatcatcaa cgcagattat taatctgctg gtatggagcg ggctgtgtgg
    21121 ctggataaat cgcttaaaaa tcgcgttggg tgagacatat taaccttgcc gcgtcagaca
    21181 gattcgcgta aaactgtcag ccgctctaat ggccaccaaa atagacaatt atgtttcagg
    21241 acaacccgct gctagcgcag cttaaacagc aactgcattc ccagacgcca cgcgctgaag
    21301 gggtggtaaa agccacagaa aaaggctttg gcttcctgga agtcgacgcg caaaaaagtt
    21361 atttcattcc gccgccgcag atgaaaaaag tcatgcatgg cgaccgaatt atcgcggtga
    21421 tccacagtga aaaagaacgt gaatccgcag agccagaaga actggttgaa ccgttcctga
    21481 ctcgtttcgt gggtaaagtt cagggcaaaa atgaccgtct ggccatcgtt cctgatcatc
    21541 cactcttaaa agacgccatt ccttgccgcg cagcccgtgg cctgaaccac gagtttaaag
    21601 aaggcgactg ggcggttgcc gaaatgcgcc gtcatccgct gaaaggcgat cgttctttct
    21661 atgctgaatt gacacaatac atcacttttg gtgacgacca ctttgtaccg tggtgggtta
    21721 cacttgcgcg ccataatctg gaaaaagaag caccagacgg cgtcgctacc gaaatgctcg
    21781 atgaaggtct ggttcgtgaa gatctgaccg cgctggattt tgtcaccatc gacagtgcca
    21841 gcacagaaga tatggatgac gcccttttcg ctaaggcgtt gccggatgac aaacttcagc
    21901 tgattgtggc gattgccgat ccaaccgcgt ggattgctga aggcagcaag ctggacaaag
    21961 ccgcgaaaat tcgcgcattc accaactatc tgcctggctt caacatccct atgctgcctc
    22021 gcgagctttc tgacgatctc tgctcactgc gcgccaatga agtccgcccg gtactggcat
    22081 gccgcatgac gctctccgct gatggcgcca ttgaagataa tatcgaattc tttgccgcca
    22141 ccatcgaatc caaagcaaag ctggtgtatg accaggtttc tgactggctg gaaaataccg
    22201 gtgactggca gcctgaaagt gaagcaattg ccgaacaagt ccgtttgcta gcgcaaattt
    22261 gccaacgccg cggcgagtgg cgtcataacc acgcactggt gtttaaagat cgcccggatt
    22321 accgctttat tctcggtgaa aaaggtgaag tgctggatat cgtcgccgag cctcgtcgca
    22381 ttgccaaccg tatcgtcgaa gaagcgatga ttgccgctaa catttgtgcg gcccgcgtac
    22441 tgcgcgataa gctcggtttt ggcatctata acgtgcatat gggctttgat ccggcgaatg
    22501 ccgacgcgct ggcagcgttg ctgaaaacgc acggtctgca tgtcgatgcc gaagaagtgc
    22561 tcacgctgga cggtttctgc aaactgcgtc gtgaactgga cgcgcaacca actggtttcc
    22621 tcgacagccg cattcgtcgc ttccagtcat ttgctgaaat tagcactgaa cccggtcctc
    22681 actttggcct cggtctggaa gcatacgcca cctggacttc gccgatccgt aaatatggcg
    22741 acatgatcaa ccaccgtctg ctgaaagcgg ttatcaaagg cgaaactgcg acgcgcccac
    22801 aggatgaaat cactgtccaa atggccgagc
//
LOCUS       DAJTYD010000050        22707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTYD010000050 DAJTYD010000000
VERSION     DAJTYD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.050"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..625)
                     /gene="fsr"
                     /locus_tag="OO585_001765"
     CDS             complement(<1..625)
                     /gene="fsr"
                     /locus_tag="OO585_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6629384.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMN"
     gene            843..2495
                     /gene="ushA"
                     /locus_tag="OO585_001766"
     CDS             843..2495
                     /gene="ushA"
                     /locus_tag="OO585_001766"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6629385.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2532..3011)
                     /gene="ybaK"
                     /locus_tag="OO585_001767"
     CDS             complement(2532..3011)
                     /gene="ybaK"
                     /locus_tag="OO585_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6629386.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3215..4009)
                     /locus_tag="OO585_001768"
     CDS             complement(3215..4009)
                     /locus_tag="OO585_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6629387.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4147..4488
                     /locus_tag="OO585_001769"
     CDS             4147..4488
                     /locus_tag="OO585_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6629388.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4703..7207)
                     /gene="copA"
                     /locus_tag="OO585_001770"
     CDS             complement(4703..7207)
                     /gene="copA"
                     /locus_tag="OO585_001770"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6629389.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPLW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7469..8401
                     /gene="glsA"
                     /locus_tag="OO585_001771"
     CDS             7469..8401
                     /gene="glsA"
                     /locus_tag="OO585_001771"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6629390.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8404..9696
                     /locus_tag="OO585_001772"
     CDS             8404..9696
                     /locus_tag="OO585_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6629391.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9821..10228
                     /gene="cueR"
                     /locus_tag="OO585_001773"
     CDS             9821..10228
                     /gene="cueR"
                     /locus_tag="OO585_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6629392.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10229..10687)
                     /locus_tag="OO585_001774"
     CDS             complement(10229..10687)
                     /locus_tag="OO585_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6629393.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10684..11601)
                     /locus_tag="OO585_001775"
     CDS             complement(10684..11601)
                     /locus_tag="OO585_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6629394.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11747..12424
                     /gene="fetA"
                     /locus_tag="OO585_001776"
     CDS             11747..12424
                     /gene="fetA"
                     /locus_tag="OO585_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6629395.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12411..13190
                     /gene="fetB"
                     /locus_tag="OO585_001777"
     CDS             12411..13190
                     /gene="fetB"
                     /locus_tag="OO585_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6629396.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13253..14107)
                     /gene="cnoX"
                     /locus_tag="OO585_001778"
     CDS             complement(13253..14107)
                     /gene="cnoX"
                     /locus_tag="OO585_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6629397.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14168..14977)
                     /gene="ybbO"
                     /locus_tag="OO585_001779"
     CDS             complement(14168..14977)
                     /gene="ybbO"
                     /locus_tag="OO585_001779"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6629398.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(14967..15590)
                     /gene="tesA"
                     /locus_tag="OO585_001780"
     CDS             complement(14967..15590)
                     /gene="tesA"
                     /locus_tag="OO585_001780"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6629399.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15561..16247
                     /locus_tag="OO585_001781"
     CDS             15561..16247
                     /locus_tag="OO585_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6629400.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16244..18655
                     /locus_tag="OO585_001782"
                     /pseudo
     CDS             16244..18655
                     /locus_tag="OO585_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19084..22707
                     /locus_tag="OO585_001783"
                     /pseudo
     CDS             19084..22707
                     /locus_tag="OO585_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5227 a         5961 c         6428 g         5091 t
ORIGIN      
        1 tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca
       61 gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg
      121 ccgtcagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg
      181 ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga
      241 ccgatgaact ggtaccgacc agcgccgccg ccagcagaac tgcgccaaca ctgcccgcca
      301 gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca
      361 tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga
      421 aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagag
      481 gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag
      541 cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag
      601 gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga
      661 atgatcttat ttgtgattat tacccgacta acatacctgt aagcgtcgcc tgaaggaagt
      721 ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc ggacgcagac
      781 ttgtctatta tgttgcgtca tactattttt caacacgttg aaatcaggtc agggagagaa
      841 gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt acactggcga
      901 gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt ctgcatacca
      961 atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg gcggcgcaaa
     1021 aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc gtgctgctac
     1081 tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat gccgaacctg
     1141 attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat catgaatttg
     1201 ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg ttgctttccg
     1261 cgaatatcta ccagaaaagt accggcgagc gcctgtttaa accatgggcg ctgtttaagc
     1321 gtcaggatct gaaaattgcc gtcattgggc tgacaaccga tgacacagca aaaattggta
     1381 acccggaata cttcactgat gtcgaatttc gtaagcccgc cgatgaagcg aagctggtga
     1441 ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc catatggggc
     1501 attacgataa tggtgagcac ggctctaacg caccgggtga tgtggagatg gcacgcgcgc
     1561 tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagctccg gtctgcatgg
     1621 cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa ccagatcaac
     1681 aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga cgggctgatt
     1741 ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg gtgaacctga
     1801 agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg
     1861 ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca gaacaaaggc aaagcgcagt
     1921 tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt
     1981 ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc actggtgccg
     2041 actttgcggt gatgagtgga ggaggaattc gtgattctat cgaagcaggc gatatcagct
     2101 ataaaaacgt actgaaagtg cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg
     2161 gtaaagaggt gattgattac ctgaccgccg tcgcacaaat gaagccagat tcaggtgcct
     2221 acccgcaatt tgccaacgtt agctttgtga cgaaagacgg caaactcaac gacctgaaaa
     2281 tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg
     2341 ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg aataccggct
     2401 ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg
     2461 tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga tatttgcatc
     2521 cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag gatcttcgcc
     2581 agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccagaaac ataaatagtg
     2641 gcaaattctt gtgcgggggc gtcgataatc gtcggcagac gttttttctg ccccagtggg
     2701 ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg ccatcggatc ggccatctca
     2761 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
     2821 gtaacggcca ctgccagatg tttcatatca ccgttcactg ccaccagtag cgttttgtag
     2881 acttgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagcc
     2941 ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag taatttaact
     3001 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
     3061 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
     3121 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
     3181 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc ggcaaattcc
     3241 cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc ggcatggcgc
     3301 gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac agcgactgac
     3361 tgaacgtatt gggcagcgtt atatcattat tttgcggagg tgcattcagc caccagctca
     3421 tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg tccagcagcg
     3481 ccagtccttt gtcagggagc tggagcaaca tggtaatctg gttttcagcc ccttccagtt
     3541 caatcacggg tttatgttgt tgcttcgccg cctgcaataa ctggtaatcg ataccgtatt
     3601 ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca
     3661 gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcagat
     3721 tttggagttg ctcctcgtta atgcgctctt ccagcgcctc gcaagtaggc aaattagcaa
     3781 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
     3841 ttttgagcaa acgggtgggc aggggagtca tatcgtggct acccatatga atactgccaa
     3901 tcagatgaaa atggcgattg ccgggaaggg tgatatcgat ggcaggccag gtgtaatgat
     3961 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
     4021 ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg gggacttgtt
     4081 taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa gcaacagtta
     4141 aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa
     4201 tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt
     4261 aatagcgtca gtacactgat caataataat cgtaaactca ccactgagat ggcattccgt
     4321 ctggcgaaag tttttgatac cacagtcgat ttttggctaa acctccaggc ggcagttgat
     4381 ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat tgaaacagtg
     4441 gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc
     4501 gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga gcaccatagc
     4561 ctgatgcgac gcttacccgt cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg
     4621 ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc cgccctaaag
     4681 cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac cggttggcgt
     4741 tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc gggttaagca
     4801 gtgttccagt aaacggccac aaaataccag cggcgaccgg aataccaatg ctgttgtaga
     4861 taaacgcacc gagcaggttc tgcttcatat tgcgcagcgt tgcgcgggaa atcgcgagcg
     4921 catctgcaac acccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa
     4981 catcactgcc gccacccatc gcaataccga catccgcctg agccagcgct ggcgcgtcgt
     5041 taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt
     5101 cggctttacc gtctggcagc accccggcga ttacctcatc aatccctgct tctttggcga
     5161 tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat cccgctttat
     5221 gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca gccagcaggg
     5281 ctactacttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat gcctgagctg
     5341 aaatatcggc ttcaatcgct ttagtatcga cttgctggtc atttaacagc gccggattac
     5401 ccagcaataa cgcatgacct tcagcttcac cgcttacgcc cagcccgcgc aatgtgcgga
     5461 aaccgttgac ctgcggcagc tgcatatcgc ctgctttatc gaggatcgct cgtgccagcg
     5521 gatggctgga accttgctcc agcgccgccg ccagacgcaa tgcttgcgct tcatcaacat
     5581 cagcaaatgt ttttactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat
     5641 cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca
     5701 cgccaaactc agccgcccgc ccgacgccgg aaataatcga cattggcgtc gccagcccca
     5761 gcgcacacgg acaggcaata atcagcaccg tggtggctat caccagggta tagacaatct
     5821 gcggtgccgg accaaagaaa taccagattg ctgcactgat cagcgcaatc accactacta
     5881 ccggcacaaa tacggctgag attttatccg ccagctgacc gatttctggc ttgctgctct
     5941 gggcctggcg caccatgcga ataatccttg acagcgtggt atggctgcca accgcactgg
     6001 cgcgaaatag cacgctaccg tcctgtacca ctgtcccggc atggacgctt tcgccttcgc
     6061 ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat gcttcgccct
     6121 gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagt aacatccctg
     6181 gctgaacttc cgccagcggc acgctttttt caccctctgc ggtgaccact cgcgccgtcg
     6241 gtggggttaa atcgagtaat ttctccagcg ccttagaaga acgctgacgc gcgcgcgctt
     6301 ccagcatatg gccgagatta atcagaccga taatcatcgc actggcttca tagtaaagat
     6361 gccgcgcttc catcgggaac cacagcggcc acaggttgac gctcatcgaa tagagccacg
     6421 ccacacccgt acccagcgcc accagcgtat ccatcgtcgc cgcaccgttc agcaggcttt
     6481 tccacgcgct gcggtaaaaa tgcccgccgg cgaaaaccat taccgccagg gttatcaggc
     6541 cgataactaa ccacaggctg cggttgtcag cggtgaccat catgttatcg ccgatcatcc
     6601 cccagaccat taccggaata cccaccgcca gtgcgacaat cgcctgccag cggaagcgct
     6661 tcatcgtagc gacggcggtt tcctgctggc gctcgcggcg tttcgcgtca tcttcaatcg
     6721 cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc
     6781 ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc
     6841 tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca
     6901 actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca
     6961 gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg
     7021 cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa
     7081 cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt tcgagacttt
     7141 ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc cagggtcagg tcgatagttt
     7201 gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt taaaccttcc
     7261 agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta aagattaaaa
     7321 aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg tagttaagtt
     7381 gctggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg aattacctcc
     7441 cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac agcaggcagt
     7501 ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg attacattcc
     7561 ctttctggcg aatgtaccag gtcaactggc ggtagtggct atcgtgacct gcgatggcaa
     7621 cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct cgaaagtctg
     7681 tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca aaattggcgc
     7741 tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg gcggcaaacc
     7801 gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta acgctgaaaa
     7861 tattgaacaa cgctggcagc gaattttaca tatccaacag caactggctg gtgagcaggt
     7921 agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata accgggccat
     7981 agcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag cctgtgacgt
     8041 gtacacccgt cagtgctcca cgctcatcaa tactgttgaa ctggcaacgc ttggcgcgac
     8101 gctggcggcg ggtggtctga atccgctgac gcataaacgc gttcttcagg ccgacaacgt
     8161 gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg gtgactgggc
     8221 gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc
     8281 tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggta acagtgttcg
     8341 cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt ttaagggctg
     8401 atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg gaatgtcgtt
     8461 tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg gcaggctgcg
     8521 ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt ggcgatgttt
     8581 tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg cattatcgac
     8641 ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cgctctcgtt actgtacctg
     8701 ttgacgctgg cggtgagcat cgccatggtc gcccatgctt ttggcgctta tgccgtgcag
     8761 tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt ggggagcatt
     8821 gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct ggaagtgatc
     8881 ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg
     8941 caaccagcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt
     9001 gggataactt tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg
     9061 aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt taccacgttg
     9121 ctttatatct cgctggcact ggttttgctt agcgatgtat cggcattaga gttagaaaaa
     9181 tatgccgata ccgccgtagc gcaggctgct tctccgctgc tcggacatgt gggttatgtg
     9241 atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa cctgttcgcc
     9301 gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct aatgaataaa
     9361 tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgttgat tatgctgatg
     9421 acggcggcac tgaatttagg ctcactcgcc agcgttgcca gcgccacctt tttgatttgc
     9481 tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc ctcgttgccg
     9541 attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat ctacagtctg
     9601 tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact cagccttatt
     9661 gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg cagtacttcc
     9721 tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt gaatatctgt
     9781 tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc
     9841 aaaaattacc ggcctgacta gcaaagccat tcgcttctat gaagagaagg ggctggtgac
     9901 gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc tcaacgaact
     9961 gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg gcgagctggt
    10021 gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca cgctggagaa
    10081 ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc agctgctggc
    10141 actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg aaaatctctc
    10201 cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata tgtagggtta
    10261 tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg ctggcgctga
    10321 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
    10381 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta tcgctgtgct
    10441 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
    10501 ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag accaccaggc
    10561 cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc agcatctcgg
    10621 ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg accattaact
    10681 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agttcggcaa tcccggcaat
    10741 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
    10801 accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat
    10861 atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg cttcggcgga
    10921 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
    10981 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
    11041 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
    11101 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
    11161 ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
    11221 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
    11281 cgccagctcc agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa
    11341 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
    11401 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
    11461 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
    11521 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
    11581 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
    11641 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
    11701 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
    11761 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa
    11821 catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
    11881 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
    11941 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccaac aagtctctta
    12001 ctgcgcccag acaccgacgc tgtttggcga tacggtatac gataatctga tctttccttg
    12061 gcagatccgt aaccggcagc ctgacccagc catttttctc gattttctcg aacgcttcgc
    12121 cttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg aaaaacaacg
    12181 catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac
    12241 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg
    12301 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
    12361 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
    12421 ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg gcaatcttaa
    12481 ttagccataa agaaaaactg gcgctggaga aagatatttt ctggagcgtc gggcgcgcga
    12541 taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
    12601 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
    12661 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gttggggcgg
    12721 gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga
    12781 tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
    12841 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
    12901 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
    12961 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
    13021 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
    13081 tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt
    13141 tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
    13201 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg aacaacggaa tatcaataca
    13261 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
    13321 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
    13381 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
    13441 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
    13501 gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt
    13561 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
    13621 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
    13681 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
    13741 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
    13801 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
    13861 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
    13921 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg
    13981 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
    14041 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
    14101 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
    14161 cttcaactca cccctgcaat attttgtcca tcacgcgccc aggcagcagg cgcttgagca
    14221 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
    14281 aagcatggcg cactttgtcg accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
    14341 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
    14401 gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca
    14461 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
    14521 tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca
    14581 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
    14641 gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt
    14701 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtctgct gcgcgatcaa
    14761 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct
    14821 caacatcatc cggttttcgg caacctgcca gcacatgaaa accctggcgt tttaattcga
    14881 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
    14941 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
    15001 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
    15061 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
    15121 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
    15181 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
    15241 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg
    15301 ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc
    15361 gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga cgttttactc
    15421 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
    15481 ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa ggttaacagg
    15541 accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
    15601 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
    15661 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
    15721 cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc
    15781 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt
    15841 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggctc
    15901 tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt
    15961 tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac
    16021 gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc gacgaaccca
    16081 ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
    16141 gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg
    16201 accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg cacgttggtt
    16261 ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt
    16321 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccagca
    16381 aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc
    16441 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
    16501 aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta
    16561 cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt
    16621 attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt
    16681 gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa
    16741 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
    16801 cgtgcagccg gggagtcggg tcacctggcg ttataaattc ggcggcaacg agaaccagct
    16861 cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct
    16921 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
    16981 ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtagcga tgaatcatta
    17041 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgaacgca
    17101 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg
    17161 tggggccata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
    17221 tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgcttggca ccatgacggt
    17281 catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg
    17341 cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc tgccgattgt
    17401 cagtatggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc
    17461 ggtgctggcg ggcgcagtag tactggcttt gctgtgcggt gtgctgggct ggatgcagct
    17521 gaatgtactt tgccgcatga cgctgaaatc gctacctctg cgcctggcgg ttagccgcct
    17581 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
    17641 gctggcgctg ttgctggtat tgcgttgcga tctgctcgac cgctggcagc agctaccgcc
    17701 agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac cgctaaaagc
    17761 gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc gggcgcggct
    17821 gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta atcgcgaact
    17881 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    17941 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    18001 cggtggtacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    18061 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    18121 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    18181 gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagatattg gcgcgatttt
    18241 aaaacaggtc ggttaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    18301 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg
    18361 tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac
    18421 attgtggtgt gagttcgcca tgcttgggtt tgtttccggc ctggtggccg caattggtgc
    18481 ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat ttcccgtggg agccagactg
    18541 gcgattgtgg attgttctgc cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg
    18601 gctgggtgcg cgactggtta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaga
    18661 gtaaataagg ggcgttctgg ttgaatggaa cgccttgttt aaattaattc tacaatatgt
    18721 ttcattgatg atgttattga attggtgatt tcctatcttt ctattgctga tattaatctg
    18781 aattggtgga gtttaataat tttaaatata gccctacaaa aataactcag actaataaaa
    18841 tcataaatca tatgcgttga atggatatta tccatatagt gaatttgttg atgatgaatt
    18901 catctgtgct aaaaatttta gtttaataaa atattgaaag tgacctgtaa taacagttat
    18961 tgttgattga gaacaaataa gtttatgtga aaaatatata aatacattag ctggtcatgt
    19021 gtgtcatttt atttttttgt tgctaacaca gggatatgaa caataactaa aagggcactt
    19081 tatatgagcg gaaaaccagc ggcgcgtcag ggagatatga ctcagtatgg cggtcccatt
    19141 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
    19201 ccgggcggga tgacctcggg caacccggtg aacccgctgc tcggtgcaaa agtacagccc
    19261 ggcgagacgg accttgcgct gcccgacccg ctgccgttca ttctctcccg cacctacagc
    19321 agctaccgga cgaagacgcc tgcgccagtg ggaacttttt ggccccggct ggaaagcgcc
    19381 ttctgatatc cgcttacaga tacgcgatga tgcactggta ctcaacgaca acggcgggcg
    19441 gagcattcac tttgagccgc tgctgccggg gaaggctgtg tacagccgca gtgagtcaat
    19501 gtggctggtg cgtggcggta aggcagcgca gccggacggc cacacgctgg cacgactgtg
    19561 ggcgtcgctg ccgccggata tccggttaag cccgcatctt tacctggcga ccaacagcgc
    19621 acaggggccg tggtggatac tggggtgatc tgagcgggtg ccgggtgctg aggacgtact
    19681 gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggaccgct tcgggcggac
    19741 gctgacgtac cggcgtgagg ccgcaggcga cctggccggg gaaatcaccg gcgtgacgga
    19801 cggtgccggg cgggagttcc gtctggtgct gaccacacag gcgcagcagg cggaagaggc
    19861 ccggcagcag gcggtcgtcg ccggagcaaa acgaccggac attcctgatt cactgccgga
    19921 gagcacagag tacggccccg acaggggtat ccgcctttcg gcggtgtggc tgatgcacga
    19981 cccggcatac ccggagagcc tgcccggtgc gccactggcg cggtacacgt atacggaagc
    20041 cggtgaactg ctggcggtat atgaccgcag caatacgcag gtgcgcgctt tcacgtatga
    20101 cgcgcagcac ccgggccgga tggtggcgca ccgttacgcg ggaaggccgg agatgcgcta
    20161 ccgctacgac gatacggggc gggtggtgga gcaactgaac ccggcagggt taagctaccg
    20221 ctatctttat gagcaggacc gcatcaccgt caccgacagc ctgaaccggc gtgaggtgct
    20281 gcatacagaa ggcggggccg ggctgaaacg ggtggtgaaa aaagaactgg cggacggcag
    20341 cgtcacgcgc agcgggtatg acgcggcagg aaggctcacg gcgcagacgg acgcggcggg
    20401 acggcggaca gagtacggtc tgaatgtggt gtccggcgat atcacggaca tcaccacacc
    20461 ggacgggcgg gagacgaaat tttactataa cgacgggaac cagctgacgg cggtggtgta
    20521 cccggacggg ctggagagca gccgggaata tgatgaaccg ggcaggctgg tatcggagac
    20581 atcgcgcagc ggggagacag tacgctaccg ctacgatgac gcgcacagtg agttaccggc
    20641 gacgacaacg gatgcgacgg gcagcacccg gcagatgacg gcggttcacc gcgaggaagg
    20701 tatcagcctt taccgccact atgacaaccg tggccggtta acctcggtga aagacgcaca
    20761 gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac ctgactgccg ttatcacccc
    20821 ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca gcaccacgca
    20881 gggcgggctg acgcgcagta tggagtacga tgctgccgga cgtgtcatca gtctgactaa
    20941 cgagaacggc agtcacagcg acttcagcta cgatgcgctg gaccggctgg tacagcaggg
    21001 cggctttgac gggcggacgc aacgttatca ttatgacctg accggaaaac tcacgcagag
    21061 tgaggatgag gggcttgtca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
    21121 gacggtgaac ggcgacccgg cagagcagtg gcagtatgac ggccacggct ggctgagaga
    21181 aatcagccac ctgagcgaag gccaccgtgt cgctgtccac tacggctacg acgataaggg
    21241 ccgcctgacg ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca
    21301 gcatgagacg aaacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag
    21361 cctgccgccg gtggagtggc tgacctacgg cagcggttac ctggcgggca tgaagctggg
    21421 cgggacgccg ctggtcgagt atacgcggga caggctgcac cgtgagacgg tgcgcagctt
    21481 cggcagcatg gcaggcagta atgccgcata tgaactgacc agcacataca cccccgcagg
    21541 ccagttacag agccagcacc tgaacagcct ggtgtatgac cgtgactacg ggtggaatga
    21601 caacggcgac ctggtgcgca tcagcggtcc gcgacagacg cgggaatacg gctacagcgc
    21661 cacgggcagg ctggagagcg tgcgcaccct cgcaccggac ctggacatcc gcatcccgta
    21721 tgcgaccgac ccggcgggaa accggctgcc ggacccggag ctacacccgg acagcacgct
    21781 cacggcgtgg ccggataacc gcatcgcaga ggatgcgcac tatgtttacc actatgatga
    21841 atacggcaga ctgacggaga agacggaccg catcccggcg ggtgtgatac ggacggacga
    21901 cgagcggacc caccactacc actacgacag ccagcaccgc ctggtgttcc acacgcggat
    21961 acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctga gccgccggac
    22021 ggggaaacgg gtgtggcgtc gcgagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
    22081 accggaggtg acgtggtacg gatgggacgg cgaccggctg acgacggtac agaccgacac
    22141 cacgcgtatc cagacggtat accagccggg gagcttcgcg ccgctcatcc gcatcgaaac
    22201 agacaacggt gaacgggaga aagcgcagtg ccgcagcctg gcggagaagt tacagcagga
    22261 agggagcgaa gacggtcacg gtgtggtgtt cccggctgaa ctggtggggc tgctggacag
    22321 gctggagggg gaaatccggg caaactgcgt gagcagtgaa agccgccagt ggcttgcgca
    22381 gtgcgggctg acggtggagc ggctggcggc acagatagag ccggtttatt tgcctgagcg
    22441 aaaaatacat ctttatcact gtgaccaccg gggcctgccg ctggcgctta tcagtgaaga
    22501 cggcaatacg gcgtggagcg cggaatatga tgaatggggc aaccagctta atgaggagaa
    22561 cccgcatcac ctgcaccagc cgtaccggct gccggggcag cagtatgata aggagtcggg
    22621 gctgtactac aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga
    22681 cccgataggg ctggaggggg gatggag
//
LOCUS       DAJTYD010000051        22669 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTYD010000051 DAJTYD010000000
VERSION     DAJTYD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22669
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.051"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            5..577
                     /locus_tag="OO585_001784"
     CDS             5..577
                     /locus_tag="OO585_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6629401.1"
                     /translation="MGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATVQQASEKV
                     IWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSIRRRINHP
                     DRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGN
                     MTRSSPSASAVKRLMNCYGGSLTLPDLFWG"
     gene            532..660
                     /locus_tag="OO585_001785"
                     /pseudo
     CDS             532..660
                     /locus_tag="OO585_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
     gene            703..1317
                     /gene="dmsD"
                     /locus_tag="OO585_001786"
     CDS             703..1317
                     /gene="dmsD"
                     /locus_tag="OO585_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6629402.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            1476..2768
                     /gene="clcB"
                     /locus_tag="OO585_001787"
     CDS             1476..2768
                     /gene="clcB"
                     /locus_tag="OO585_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6629403.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(2721..3416)
                     /gene="bioD"
                     /locus_tag="OO585_001788"
     CDS             complement(2721..3416)
                     /gene="bioD"
                     /locus_tag="OO585_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6629404.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3541..4761)
                     /gene="mlc"
                     /locus_tag="OO585_001789"
                     /gene_synonym="dgsA"
     CDS             complement(3541..4761)
                     /gene="mlc"
                     /locus_tag="OO585_001789"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6629405.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPIISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            complement(4896..5789)
                     /locus_tag="OO585_001790"
     CDS             complement(4896..5789)
                     /locus_tag="OO585_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6629406.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            5896..7148
                     /locus_tag="OO585_001791"
                     /pseudo
     CDS             5896..7148
                     /locus_tag="OO585_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            7545..7859
                     /locus_tag="OO585_001792"
     CDS             7545..7859
                     /locus_tag="OO585_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6629407.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            7952..8035
                     /locus_tag="OO585_001793"
     CDS             7952..8035
                     /locus_tag="OO585_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629408.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            8135..8956
                     /locus_tag="OO585_001794"
     CDS             8135..8956
                     /locus_tag="OO585_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6629409.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(8995..9324)
                     /gene="mdtI"
                     /locus_tag="OO585_001795"
     CDS             complement(8995..9324)
                     /gene="mdtI"
                     /locus_tag="OO585_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6629410.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9311..9676)
                     /gene="mdtJ"
                     /locus_tag="OO585_001796"
     CDS             complement(9311..9676)
                     /gene="mdtJ"
                     /locus_tag="OO585_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6629411.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            10088..11122
                     /gene="tqsA"
                     /locus_tag="OO585_001797"
     CDS             10088..11122
                     /gene="tqsA"
                     /locus_tag="OO585_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6629412.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(11147..12535)
                     /gene="pntB"
                     /locus_tag="OO585_001798"
     CDS             complement(11147..12535)
                     /gene="pntB"
                     /locus_tag="OO585_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6629413.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(12546..14078)
                     /gene="pntA"
                     /locus_tag="OO585_001799"
     CDS             complement(12546..14078)
                     /gene="pntA"
                     /locus_tag="OO585_001799"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6629414.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            14602..15546
                     /locus_tag="OO585_001800"
     CDS             14602..15546
                     /locus_tag="OO585_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6629415.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            15732..17114
                     /locus_tag="OO585_001801"
     CDS             15732..17114
                     /locus_tag="OO585_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6629416.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWFVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            17151..17873
                     /gene="folM"
                     /locus_tag="OO585_001802"
     CDS             17151..17873
                     /gene="folM"
                     /locus_tag="OO585_001802"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6629417.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17870..18182)
                     /locus_tag="OO585_001803"
                     /pseudo
     CDS             complement(17870..18182)
                     /locus_tag="OO585_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
     gene            18308..19027
                     /gene="rstA"
                     /locus_tag="OO585_001804"
     CDS             18308..19027
                     /gene="rstA"
                     /locus_tag="OO585_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6629418.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            19031..20332
                     /gene="rstB"
                     /locus_tag="OO585_001805"
     CDS             19031..20332
                     /gene="rstB"
                     /locus_tag="OO585_001805"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6629419.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            20408..21337
                     /gene="tus"
                     /locus_tag="OO585_001806"
     CDS             20408..21337
                     /gene="tus"
                     /locus_tag="OO585_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6629420.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHCDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(21334..>22669)
                     /gene="fumC"
                     /locus_tag="OO585_001807"
     CDS             complement(21334..>22669)
                     /gene="fumC"
                     /locus_tag="OO585_001807"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6629421.1"
                     /translation="AQTQRSLEHFRISTEKMPTSLIHALALTKRAAAKVNEDLGLLSE
                     EKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQSNMNMNEVLANRASELLGGVRGME
                     RKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQLIPQLKTLTQTLSEKSRAFADIVK
                     IGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYSLPHVAELALGGTAVGTGLNTHPE
                     YARRVADELAVITCAPFVTAPNKFEALATCDALVQAHGALKGLAASLMKIANDVRWLA
                     SGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALTMLCCQVMGNDVAINMGGASGNFE
                     LNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIEPNRERINQLLNESLMLVTALNTH
                     IGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEFDSWVRPEQMVGSMKAGR"
BASE COUNT         5423 a         5860 c         5617 g         5769 t
ORIGIN      
        1 aatggtgggc atcagccgca gaacactcgt taaatcaacc gcgataggtt ctctggcgct
       61 ggctgcaggc ggtttttctt taccgttcac cctgcgcagt gcagcggcaa cggtacaaca
      121 ggccagcgaa aaagtgatct ggggtgcctg ttccgtcaac tgtggtagcc gctgtgcact
      181 tcgtctacat gttaaagata atgaagtgac ctgggtggaa actgacaata ccggcagcga
      241 tgagtacggc aaccatcagg tacgcgcctg tttgcgcggt cgctccatcc gccggcgtat
      301 taatcatccc gatcgcttga attacccaat gaaacgcgtg ggtaaacgcg gtgaaggcaa
      361 attcgaacgg attagctggg atgaagccct ggatactatc gccagtagcc tgaaaaaaac
      421 cgtcgaacaa tatggcaatg aggctgtata tattcagtac tcttcgggga ttgttggcgg
      481 caatatgacc cgctcttcgc catcagcctc ggcggtcaaa cgcctgatga actgctacgg
      541 cggttccctc acgttgccgg acttattctg gggctaatcc tcatacttgg cggtgagatg
      601 atcgggcgtg tgctctttta tggcctgcac atgacagtag gaatggctat tgcaggttaa
      661 caaattggtg cggggcaacc cgcacatttc aggatgttag gaatgaccca tttttcacag
      721 caagataatt tttctgtcgc ggcgcgcgtg ttgggcgcgc tgttttatta cgctccagag
      781 agtgcggaag ccgcacctct ggttgcggta ctcaccagtg acggctggga aactcagtgg
      841 cctctaccag aagcgtcatt agcgccgctg gtgacggctt ttcagacaca gtgtgaagag
      901 actcacgccc aggcctggca gcgtttgttt gtcggcccgt gggcactgcc gtctccgccg
      961 tgggggtcgg tctggctgga tcgcgaatct gtgctgtttg gcgattcaac attggcactt
     1021 cgtcagtgga tgcgcgagaa aggcattcag tttgaaatga agcaatacga accggaagat
     1081 cattttggtt cactgctgtt gatggctgcg tggttggcgg agaatggtcg ccagacagaa
     1141 tgcgaagaat tgctggcatg gcaccttttt ccgtggtcaa cacgttttct tgatgttttt
     1201 atcgaaaaag cagaacaccc tttctaccgt gcactgggtg aactgtctcg cttaacgctg
     1261 gcgcaatggc aatcacaact gttaattcct gtcgcggtta aaccgctgtt tcgataggat
     1321 caccgtaata ttgccggatg gtgacgatta cgcgcaatcc ggcaataata ggttacagtg
     1381 tcacgttttt ttatctctta aagcacgcac tgcttttgcg gctggcctct tttgccgcaa
     1441 aatagtcgcc cgtgtttcat tgcccatttc tgctcatgca tcatctacac atctatccgg
     1501 atctgcgcac tatgttccgc cgtctgctta tcgcaacagt cgtcggtatt ctcgcggcct
     1561 ttgccgttgc cgggtttcgt catgcgatgc tgctactgga gtggttgttc ctcaataatg
     1621 actccggcag tctggtcaat gcagcgacaa acctttcccc ctggcgacgg ttgctaactc
     1681 cggcgctcgg cggactggcg gcgggtttgt tgctgatggg ctggcagaaa tttacccaac
     1741 aacgccctca tgcgccgacc gattacatgg aagcgttgca aaccgatgga cagttcgatt
     1801 acgcagcaag cctggttaaa tcgcttgcct cgctgctggt agtaaccagc ggcagtgcaa
     1861 ttggtcgcga aggtgcgatg attcttttag ctgcccttgc cgcctcctgt tttgcccaac
     1921 gttttacgcc acgccaggag tggaaattat ggatcgcctg tggcgccgcg gcgggaatgg
     1981 ctgcggccta tcgtgccccg cttgctggca gtttatttat agccgaagtg ctgtttggca
     2041 ctatgatgtt ggcctctctc ggcccggtga ttatttccgc cgtcgtggca ttgctggtta
     2101 gcaatctgat taatcatagc gacgcgttac tctacaacgt acaactctca gtgacggttc
     2161 aggctcgtga ctatgcgctg attatcagta caggtgtgct ggcaggtctg tgcggaccac
     2221 tgttgttaac gttaatgaac gcctgtcatc gtggatttgt gagtctcaaa cttgcgccgc
     2281 cctggcaact ggcactaggc gggttgatcg tgggtctgct ttccctgttc acacctgcag
     2341 tgtggggcaa cggctatagc accgtacaat cctttttaac cgccccacca ctgttaatga
     2401 tcattgccgg gatcttcctt tgtaaactgt gtgccgtgct ggcgagtagc ggttccggcg
     2461 cacccggtgg tgtctttaca ccgacgctat ttatcggtct tgccattggc atgttgtatg
     2521 gtcgtagcct gggattatgg ttccctgatg gcgaagaaat tacgctttta ctcggattga
     2581 ccgagatggc gacactgttg gcggcaacca cgcacgcgcc gattatgtcg acgttgatga
     2641 tatgtgaaat gaccggggag tatcagctac tccccggttt attgattgcc tgcgtcattg
     2701 cgtcggtaat ttcgcggacg ttacaccgtg actctatcta ccgccagcac actgcgcagc
     2761 atagctaagc ggatgtattg ccccagttct cgctgttcgg cgcgcggcag ataaggcagt
     2821 tcaccaatga gcggtgccgg aagtttttta cccagcacat caatgatttc cgcataatgc
     2881 gccagtcctg ggttgattcg gttagccacc cagccaatga gcggcagccc gtcgttggca
     2941 atcgcctgag ctgttagcag tgcatggtta atgcaacctt cctgaatacc gacaaccatc
     3001 aacaccggca actgttcctg caccacccat tcagagagtg gacgcaaatc attcatcaga
     3061 ctgcgccagc cgccagtccc ttctaccacg acatgatcga ctttatcggt caggtttgcc
     3121 aggccgtttg aaatgagggt gtaattgatt gggcaactgt gcgccacgct actttcttct
     3181 tcgcttaacg cgataggatt aactgcttca taaggtagtt cgatggttga aacactctgc
     3241 aacaccaggg catctttatt acgcagccct tcgggtgtct ctttgctccc cttcgctacg
     3301 ggtttatatc ccgcaactgt ttttccctgg gaggctaacg cttgtagcaa tgcgcgggaa
     3361 accaccgttt tccctacaga agtgtctgta ccggtaataa agaaacgctt cagcatcact
     3421 aactccaccg ttatgcttca caaatataaa ctaggaaaat aattaacctt gaaagtctaa
     3481 gttatgcttt cctggcccaa attgagatag cgcaaatttt ggtagaacag ttaaaaaatg
     3541 ttaaccatgc aacagacgaa tcaacaaaga accgttatac atcgcgtctt ttaccagcgc
     3601 agcgcctgcc atcgtgccct ggttagaaaa ctgagtactc tcaacgctaa tgtgctgact
     3661 atacgtagga agggcctgct gacgtatgct gtccgagatg atagggaaga ggatatctgc
     3721 cgctttactt aacggtgagc caatcagtat tttttgtggg ttaaataaat tcaccatgat
     3781 ggcaagaatg cgcccgacat gcgcgcccac cccggtaatg atgtcttttg ccagtagatc
     3841 gccgcgcaat gccgcctgac acaatgagtc cacggttaac ggttgcccat gtaacatcga
     3901 gctcatggat tgattgagac gcagctgtgc cagctcaaga atactgtcca cactggcgat
     3961 ggtttcgagg cagccgaggt tcccgcaata acagcgttta ccatatgggt cgacctgtgt
     4021 gtggcctatt tccacgagac tactgctgcc tgcgtgtagc agatgaccat cggtaatgac
     4081 gcccgccccc acgttgtgat cgataaccac ctgaatcaca tcgcgcgccc cgcgtgaggc
     4141 accaaacaag gcctctgcca tcgtccatgc gctgatatca tgctgaatat aaaccggtac
     4201 gccggtatgc tgctccagcg cctcgccgag cggcatctct tttacatcct cgtagaacgg
     4261 catgcgatgt acaataccat tttccgtatc aataattccc ggcagggtta tggcaatcga
     4321 agttagacgc tcaagttttt tctggtggcg gataaaaaac tgatcgatat gggaaatgat
     4381 acgatccagc aatggcaagt catcttttaa cgccagttcc tgcgactctt ccaccaccag
     4441 tttgctgctc agatcgcgca gagcaaggaa aatctcaccg cgactaatgc gcagagaaag
     4501 atagtgccag gcttcagttt caaccaccag tcccaccgcc ggacggccac ggttccccgc
     4561 ttctttgatt tccagctctt gcaccaagtg tgcttcgagc atctcacgga caattttagt
     4621 gatactggca ggagccagct gcgccagacg ggaaagatcg atacgcgaga ctggaccaag
     4681 ctgatcaatc aggcgataaa ccgcgcccgc gttggtctgc tttatttgat caatgtgccc
     4741 aggctggttt tcagcaacca ccgcatactc cctatatttt cgcgctccga aataatctgt
     4801 aggctatggt gaagcacttc aatacgtgtc gtcaaatttt tacttagaca tgtgattaac
     4861 agcacatttt tcgggctttt tcgctgaaat ttccctcacc tgagagcatt cagcagatga
     4921 atacgaaagt tacgcgaagc tggactttgt tcatgatgtt tcggccagac caaccacatt
     4981 tcagaaaccg catcctcttc agcaatcggc acccagcgca tttcgttgag ctgagcacgt
     5041 ttaaatgaag caggcaaaat tgaaacaccc agaccggcgg aaaccagacc gatgatggtc
     5101 attgcctcgc ccacctcctg agtgatgacg ggcgtcaaat ggtaacgtcg catcagcccg
     5161 agaatatcgt catacagccc tgtcccgacg tgcggatcaa aaaagacgaa gggttctttc
     5221 gccagttcag ccaacgttac attcgggtta tttgccaggg gatgatcgtg cgggatcatc
     5281 gccataagcg gttcatggac gatgactgcg tgttcaagcg tctccggtaa cgctgtgtta
     5341 cgcagcaatc ccatatccag cgttccttca atgagcggag cgatttgctc acgagtgttc
     5401 atttcgcggg tttgtaaatg gacaccggga taatcacggc gaaacagcga taacgtatcg
     5461 gacaccgccc gaataaaagg agcggacgaa gtaaaaccaa tgcgcaactc ccccgcttca
     5521 ccctgatgca gtctttcagc gcgagcggca gcgtcatcta ccatagacag gatttgccga
     5581 ctatctgcaa gaaactgttt tcctgctgcc gtcagcaata cactgcgatt agttcgtgcc
     5641 agcagtcggg caccaatttg ttgctccagt gcctgaatct gctgacttag cggtggttgc
     5701 gaaatattca ggcgggcagc ggcgcgcccg aaatgcagct cttccgcaac agcaacaaag
     5761 taacgcagat gacgaagttc aatattcata tttaaaacat cttatttgag attattaata
     5821 tattagacag aacaattcga ttttcctaac ctatgtataa gcctgatcca caggcatatt
     5881 tagcaaggat ttcaagtgag ccgtactaca actgttgatg gcgctccggc aagcgacact
     5941 gacaagcaaa gcatttctca gccaaatcaa tttattaaac gcggtacgcc gcaatttatg
     6001 cgcgtcaccc tggcgctgtt ctctgccgga ctggcaacct ttgcactcct ctattgtgtg
     6061 cagcctatcc ttccggtgct ttcgcaggag tttggcttaa cccccgcgaa cagtagtatt
     6121 tcactgtcca tttccacggc gatgttggct attggtttgc tgtttactgg ccccctatcc
     6181 gatgccattg gtcgcaaacc agtgatggtc acggcgctac tgttggcctc aatttgtacg
     6241 ttatttcgac aatgatgacc agctggcacg gcattttgat tatgcgcgcc ttgattgggc
     6301 tttcgttaag tggcgtggca gctgttggca tgacttatct tagcgaggaa atccacccca
     6361 gtttcgtggc cttttcgatg gggttgtata tcagcggcaa ctcaattggc ggcatgagcg
     6421 gacgcttaat tagtggtgtc ttcacggact ttttcaactg gcgaattgct ctggcggcaa
     6481 tcggttgttt cgcgctagcc tcggcgttga tgttctggaa aatccttccg gaatcacgcc
     6541 attttcgccc gacttcgctg cgacctaaga cgttgtttat caactttcgt ctgcactggc
     6601 gtgaccgggg attaccgtta ttgttcgcag aaggcttttt gctgatgggg tcgttcgtca
     6661 cgctgtttaa ttacatcggc tatcggttga tgctttcccc ctggcatgtc agtcaggccg
     6721 tggttggctt attatcgctg gcttatttga ccggtacacg gagctcaccc aaagccggaa
     6781 ccatgaccac ccgctacggg cgcggtccgg tgatgttgtt ttcgacgggg gttatgctgt
     6841 ttggtttact gatgacctta ttcagctcgc tgtggctgat ctttgccgga atgttactct
     6901 tctcagcagg attcttcgca gcccactcag ttgccagcag ctggatcggc ccccgcgcaa
     6961 aacgcgctaa aggccaggcc tcctcgctgt atctgttcag ttactatctg ggatcgagta
     7021 ttgccgggac gctgggtgat gttttctggc ataactatgg ctggaacggc gtcggcgcat
     7081 ttattgctct gatgctggtc attgctctgc tggtcgggac gcgtttgcat cgtcgtctgc
     7141 acgcctgaaa aataagcccg gactgcggta actacacgtc cggacttatt gcttgctcaa
     7201 accaacgtta atagccatcc taaaatagac gaagcgccag ccaattcccg cagtgcgtct
     7261 agcgtcatca ggattataag tacccaaata aacggattca ttttgctgtg tgtcatttat
     7321 tactgatgcg cagttattct accgctttgt aagtagtaaa atagttaacc cgatcaagac
     7381 tactattatt ggtagctaaa tttcccttaa gtcacaatac gttattatca atgctgtaat
     7441 ttattcagcg tttgtacata tcgttacacg ctgaaaccaa ccactcacgg aagtctgcca
     7501 ttcccaggga tatagttatt tcaacggccc cgcagtgggg ttaaatgaaa aaacaaattg
     7561 agggtatgac aatgaaaaaa gtattagctc tggttgttgc cgctgctatg ggtctgtctt
     7621 ctgccgcctt tgctgcagag actgcgacga ccaccaaagc agcgccggcg aaaacgacac
     7681 atcataaaaa acagcataaa gcagcacctg cccagaaagc gcaggcagct aaaaagcatc
     7741 ataaaaatac gaaagctgaa cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac
     7801 acgccgggaa acacagtcat cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag
     7861 ttttcaacag taatgctggc gcgccccctc gcgcctgaaa attacggtgc taagcgggta
     7921 acgtttagca ccgcctttag cggagggtag tatggtgggc cgttatcgct ttgagttcat
     7981 tcttatcatc cttattttat gcgcactgat tgccgcccgt ttttatcttt cctgactgta
     8041 gttatctgat tttactccca ctttcatccc gtcccgtcta tagtatttat gagggtttgc
     8101 ttttaataat cataattacc caccagagtg tgatatgcgt acaaccattg ctgtagtgtt
     8161 gggtgcaatt agtttgacgt ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc
     8221 ggcaaacgat gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga
     8281 cacggcccaa tcaccgtggg atgcggttgg gcaactggaa acggccagcg gcaatttatg
     8341 tacggcgacg ctgattgcac ccaatctggc attaacggca ggacactgtt tattgacacc
     8401 tccaaagggt aaagccgata aagcagtggt gctgcgtttt gtgtcaaata aaggtctttg
     8461 gcgctatgag atccacgaca tagaaggccg cgttgatccg acactgggaa agcggttaaa
     8521 agcagatggg gatggttgga ttgtacctcc cgcagccgcg ccgtgggact tcggattgat
     8581 tgtgctacgt aatccccctt ctggcattac accgttgccg ttatttgagg gagataaagc
     8641 cgcgcttact gccgcattaa aatcggctgg ccgtaaagta actcaggcgg gataccctga
     8701 agatcatctc gatacgttgt acagtcatca aaactgtgaa gtgactggct gggcgcaaac
     8761 gtcggtgatg tcacatcagt gcgatacctt gccgggtgac agcggttcgc ctctgatgtt
     8821 gcataccaat gacggctggc aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga
     8881 tcgctggcgc gccgataacc gggccatttc tgttaccggt tttcgcgaca agctggatca
     8941 actgtcgcaa aaataatgtt caaacgctgc ccgactgtgc gggcagcgtg ttcatcaggc
     9001 aagtttcacc atgatcattc cagccaacag caagaccagg ccaatccagc ctttacgatt
     9061 taaccgttga ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac caaacccgcc
     9121 ccacaatgca taagcgacag acaagtcgat ccctttaacg gcctgagaaa gcgcactaaa
     9181 ggcagccagc accgccgcca gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga
     9241 aaatttcaaa aagacgttag caacgatttc cagcacgatt gccaatgcca gccaggcggc
     9301 gtgaacccat tcaaactgcg ccatggttta cctccagttc aggtttacgc gctttacggg
     9361 tacctgattt tatcaacaca atcccggcga ccagggtggt taacccggca attttcatca
     9421 gcgataaact ttcgtcgaat aacaaaacgc taaacaaggt aataaataaa ataccgatac
     9481 cttcccacaa cgcataagct acgcctaagg cgatcttttt aacggcgaaa gagagaaata
     9541 tatacgacag agaaatcatc accagcatta aaataaagcc gccatttccc tcactgacgc
     9601 tcgcccattt cattgacagc gtaccggtaa tttctgtagc aatagccagg cctaataaaa
     9661 tccaataaat atacattgtc cttctcctgc aagagaatta ttttaatttt cgcttaattc
     9721 agcgaaatca aagtaaagtg gtcaagcccc gcgcataggg caagcttagg cagaagaaag
     9781 gactaaagcg cgttgcgcca gtgctgctca cctacgagca agatagtgga tgaggtacaa
     9841 agatgggtaa atgttgaaaa taaggtcctg aacaaattgt ccataatatt acaattatcc
     9901 gcagtgttgc ttctcgtcat cgcggatgat aattgtcctc ggtagttgaa cacgcctgat
     9961 ttgtatcata gcttaagaat taactcaaaa tattttcact tctttacctg agcggtttga
    10021 ttttcgttat gatgacggag cgaaaaagac attattatta gcaaaggaag aaaaaacggg
    10081 gacaagcatg gcaaagccga tcatcacgct caatggccta aaaatcgtca ttatgttggg
    10141 aatgctggtc attattctct gcggtatccg ttttgccgcc gagatcatcg tgccgtttat
    10201 tctcgcatta tttattgctg ttattcttaa cccgctggtg caacacatgg tccgctggcg
    10261 agtgccgcgt gtactggcgg tgtcgatttt gatgaccatc atcgtgatgg cgatggtgtt
    10321 gctattagct tatctgggtt ccgcgctcaa cgagttgacg cggacgttac cgcaatatcg
    10381 caactctatt atgacgccgc tgcaagctct tgagccgttg ttgcaacgcg tagggattga
    10441 cgtctcagtt gaccagctgg cgcattacat tgatccgaac gcagcgatga cgttgctcac
    10501 caacttattg acgcagttat ctaatgccat gtcatcaata tttttattgc tgctgacggt
    10561 gctgtttatg ctgctcgaag tgccacaatt gcccggaaaa tttcagcaaa tgatggcgcg
    10621 tccggttgaa gggatggcgg cgattcaacg tgcaattgac agtgtttctc attatctggt
    10681 gctgaaaaca gccatcagca tcatcaccgg cctggtcgcc tgggcgatgc tcgccgcact
    10741 cgatgttcgc ttcgcttttg tctggggatt gctggccttt gcgcttaatt acatccctaa
    10801 tattggttca gttctcgcgg caatcccccc tatcgctcag gtactggtgt ttaatggctt
    10861 ctacgaagcg ttgctggtgc tggcgggata tctgctgatt aatctggtct tcggcaatat
    10921 tctggagccg cgcatcatgg gacgtgggct ggggctttcc acattggtgg tatttttgtc
    10981 gttgattttt tggggatggt tgttaggacc ggtgggtatg ctgctttccg tgccgttaac
    11041 aattattgtc aaaattgcgc ttgaacaaac agcgggaggt caaagcatcg ccgttctgtt
    11101 aagcgatctc aataaagagt gacggcctcc gcagaggccg tcagggttac agagctttca
    11161 ggattgcatc cacgctggct ttggcgtcac caaacagcat gtgggtgttt tccttgaaga
    11221 acagcgggtt ttgcacacca gcatagccag tgttcatcga acgtttaaag acaatcacgt
    11281 cctgcgcttt ccacacttcc agcacaggca taccagcaat aggactcttc ggatcatcct
    11341 gcgccgccgg gttaaccgta tcgttagcac caatcaccag tacggtatcg gtatcagcaa
    11401 agtcatcgtt gatctcgtcc atttccagca cgatgtcata cggtactttt gcttcagcca
    11461 gcaatacgtt catatgtcca ggcaaacgcc ccgcaaccgg gtggataccg aaacgcacgt
    11521 tgataccacg agcgcgcagt ttctcggtaa tttcagcgac aggatattgc gcctgcgcga
    11581 ctgccatgcc gtaccccgga gtaatgatca ctgaatggga gtttttcagc agttccgctg
    11641 tctcttctgc ggtgatttcg cggtgctcgc ccacttcctg atcatcgcca gtagaagagc
    11701 cgtcggtgcc gaaaccaccc gcaataacgc tgataaagga acggttcatc gccttacaca
    11761 taatgtaaga aaggatagcc cccgaagaac cgaccagcgc accggtcaca atcagcaggt
    11821 cgttgctgag cataaagccc gcagccgcag ccgcccagcc ggagtacgag ttcagcatcg
    11881 acaccaccac tggcatatct gcaccaccga tggaggcgac taaatgccag ccgaatacca
    11941 gcgcaatggc ggtcattatt agcaatgcca gcacttgcag gccgacgctg tccgtgcgaa
    12001 caaatacaat cagcagcagg aaggaaacga ccagagccgc caggttcatt ttgtgacggt
    12061 ttggcagcat caacggttta gacgaaatct tgccacacag tttgccgaac gccaccaccg
    12121 aacccgtgaa cgttaccgcc ccgatgaaga taccgaggaa cacttccgtc aggtgaatat
    12181 tgaccagaat cggtgccatt cccgcgtcat gatgcagata gctgttaaag ccaaccagca
    12241 ctgccgccag acccacgaag ctgtgcagga tcgccaccag ttctggcatt tcggtcattt
    12301 caactttctt cgccagacgg ataccaattg ccccaccaat gaccatcgcc agcaagatcc
    12361 agccaacatt acccgtatcc ggtccaaaaa tggttgcgat taacgcaatc gccatccctg
    12421 cgataccgaa gttgttaccc tggcgagacg tttcatgttt cgaaagaccg gccagactga
    12481 agataaacag gatcgcggca acaatgtatg cagctgtaac taatcctcca gacatatgtt
    12541 accccttaat ttttgcggaa cattttcagc atgcgctgag tcacggtgaa gccaccgaaa
    12601 atattaatgc tggctataag caccgcgata aaactaagga agctaaccca gccgccctgg
    12661 ccaatctgca acagtgctcc gacaacaata atccctgaaa tcgcgttggt gaccgacatc
    12721 aacggtgtat gcagcgcgtg cgatacattc cacaccacgt aataaccgac aacgcaggcc
    12781 agcgcgaaaa cggtgaagtg cccaagaaac tctttcggcg caacgcttgc cagccaaccg
    12841 aaaaggatga ttgccagcgc catcaacgcg tatttacgcc acggtgagca ggcacatttt
    12901 tcctcagttt tcacttccgg tgccgctttt tgtgccgcct gcggctgagc tgatacctga
    12961 atcggcggtg ccggccaggt aatttcgccc gcacggatca cggtcacgcg gcgaatcacc
    13021 acatcatcaa aatcaacagt gatattgccg tctttctctt tgcacaacag tttcagcaga
    13081 ttaacgaggt tagtaccgta aagctgcgag gattgcgtcg gcagacggcc cggaagatcg
    13141 gtataaccaa tcactttgac accattttcc gtagtgaaga tttcacccgg cacggtgtat
    13201 tcacagttgc caccgttttg ggctgccagg tcgacaatca cactgcccgt cttcatggag
    13261 tcaaccattt cacgggtaat tagcttcggc gctggtttgc ctggaataag cgcggtggtg
    13321 acaatgatat cgacctcttt tgcctgggcg gcaaagagtt ccatttctgc tttaatgaac
    13381 gcgtccgaca tcactttggc atagccatcg ccgctgcccg cttcctcttt aaaatccagc
    13441 tcgaggaatt ccgcgcccat actttgaact tgttctttca cttccggacg ggtgtcgaat
    13501 gcacgcacaa tcgcgccgag actgtttgct gcgccaatgg cggccagacc tgcaacaccc
    13561 gcaccaatca ccatcacttt tgccggtggc actttcccgg ccgcagtaat ttgcccggta
    13621 aagaagcgcc caaattcatg tgccgcttca acaatggcgc gataaccggc gatgttcgcc
    13681 atcgagctta gtgcgtccag cgattgtgcg cgtgagatac gcggcacaga atccatcgcc
    13741 atcacggtca cgttacgttc cgcaagtttt tgcattaatt ccggattctg cgcaggccag
    13801 ataaaactca ccagcgttgt cccaggattc agtaacgcaa tttcatcatc taacggcgca
    13861 ttgaccttca gaatgatctc tgactgccag acgctattcc cttctacaat ttcagcgccc
    13921 gcttgcacaa acgctttatc gtcaaaactt gccagttgac ccgcgccgct ctctaccgcg
    13981 acggtaaaac ccagtttcag cagctgttcc actgtttttg gcgttgctgc aacacgggtt
    14041 tcattggtta accgttctct tggtatgcca attcgcatga tattcccttc catcggtttt
    14101 attgatgatg gtttgcctgt gtcaggagcc acacaagccg ctcatgtacg agctaaatgt
    14161 tactccgtta aaataaatta gtaacaaacg ccctataacg tactgaaaat tatgcctgtg
    14221 atctagcgcc aaaaatcagt atttcggcgt gaactcgcaa aatattaacg attcagccgt
    14281 gatagtggga taaacacctt agaacgccgg ataaagactg ataattgtct tcgacggtcg
    14341 ggtaaaacga gacatcgccc cggcacgatt cactacttaa cattaaatta acttatacaa
    14401 ttcagttgct tcagtagtaa tgatgctgat acggctgttt tttaagcata gacggtcatt
    14461 tgagcaggat taaaattggc ttaaggaatg tgatatgaaa aatgacgcag acagttacac
    14521 cgtttaaatg caataatcag ccacgtttct cgttaataac aataccagta cctggtttgc
    14581 gcaaggcgaa ggattatttt tatgaagctt aagaacaccc tcctggcgtc ggcactgctt
    14641 tctgctacgg cattctccgt taacgcagca acagaactga caccggagca agcggcagcg
    14701 gttaaacctt ttgaccgtgt agtggttacc ggtcgtttta atgctattgg cgaagcggtg
    14761 aaagccgttt ctcgtcgcgc agataaagaa ggtgccgcct ctttttatgt tgtcgacact
    14821 tctgattttg gtaacagcgg taactggcgt gtggtcgctg acctctataa agccgatgct
    14881 gaaaaagcag aagaaacaag taatcgcgta attaacggtg ttgtcgaact gccgaaagat
    14941 caggctgttc tgattgaacc gtttgacacg gtcaccgtcc agggcttcta tcgtagccag
    15001 ccagaagtca atgatgccat caccaaagcg gcaaaagcga aaggtgccta ctctttctac
    15061 atcgttcgtc aaatcgatgc caaccagggc ggcaaccagc gtattactgc attcatctat
    15121 aaaaaagatg ctaagaaacg tatcgtccag agcccggatg tgatcccggc agattccgaa
    15181 gcaggacgtg cagctctggc tgccggtggc gaagccgcga agaaagttga gatcccaggt
    15241 gttgcgacta ccgcatcacc aagttctgaa gtcggtcgtt tctttgaaac ccagtcatca
    15301 aaaggcgggc gttacaccgt cacgctcccg gatggcacta aagtcgaaga actgaacaaa
    15361 gcgaccgcag cgatgatggt cccgttcgat agcatcaaat tctctggcaa ctacggcaac
    15421 atgaccgaag tctcctatca ggttgcgaaa cgtgccgcga agaaaggtgc taagtattac
    15481 cacatcaccc gccagtggca ggaacgtggt aataacctga ccgtcagcgc agatctgtat
    15541 aaataactat tcagttactc ctataaggcg gcttgatgcc gcctttcgca tttttagtca
    15601 caaatttccc cgcgaccatt gcatgcagac acatctcttc gtaaaatccc gcgcttcagt
    15661 ggcaaactcc atttttatgc gttttgccct aatatttatt cttcttatca cgttttaatc
    15721 actggatatc gatggaaaag aaactgggac tgagcgcact caccgcgctg gtattaagct
    15781 caatgctggg cgcgggtgtt ttcagtctgc cgcaaaatat ggcggcagtt gccagcccgg
    15841 cagcactgct catcggctgg ggtattactg gcgctggcat tttattgctg gcctttgcca
    15901 tgctgatcct cacgcgcatt cgtcccgaac ttgacggcgg tatctttacc tatgcccgcg
    15961 aagggtttgg cgagctaatc ggcttttgtt ccgcatgggg atactggctg tgcgcagtca
    16021 tcgccaacgt ctcctatctg gtgatcgttt tttccgcgtt aagctttttt acggacacgc
    16081 cggaattgcg cctgttcggc gatggtaata cctggcaatc gatcgtcggt gcatcggctt
    16141 tattgtggtt tgttcacttt ttgatcctgc gcggggtgca aaccgctgcc agcattaacc
    16201 tggtggcgac actggcaaaa ttgttgccgc tgggtctgtt tgttgtgctg gcgatgatga
    16261 tgttcaaact ggataccttc aagctcgact tcaccggact tgtacttggc gtacccgttt
    16321 gggaacaggt gaaaaacacc atgctgatca ccctgtgggt tttcattggt gtggaaggtg
    16381 ccgtggttgt ttctgcgcgt gcgcgtaata aacgtgatgt tggcaaagcg acactgctgg
    16441 cggttctctc cgctctgggc gtttacttgc tggtaacgct gctttcgctg ggtgtggtgg
    16501 cacgtcctga actggctgaa attcgtaacc cgtcaatggc cggtctgatg gtggaaatga
    16561 tgggaccatg gggcgaaatc atcatcgctg ccagtttgat cgtttccgtt tgcggcgcgt
    16621 acttaagctg gaccatcatg gcggcagaag ttccgttcct ggctgccact cataaagcat
    16681 tcccacgcat ttttgcgcgc cagaacgcac aagctgcgcc atccgcctca ctgtggttga
    16741 ccaacatctg cgtgcaaatt tgtctggtac tcatctggct gaccggttcc gattacaaca
    16801 cgttgctgac catcgcctca gaaatgattc tggtgcccta tttcctggtc ggcgcgttct
    16861 tgctgaaaat cgcaacccgt ccactgcata aagcggtagg tgtcggtgcc tgcatttatg
    16921 gcttatggtt attgtacgcc tctggtccaa tgcacttgct gttatccgtt gttctgtatg
    16981 cgccggggct gctggttttc ctgtatgcgc gcaaaacgca tacacatgat aatgtactga
    17041 accgtcagga aatggtgctg attggcatgc tgttaattgc ctcagttccg gcgacctgga
    17101 tgctggtggg ataacgttca tcccatcgtt ggatagcaaa ggagataacg atgggtaaag
    17161 cccagccctt gccaatatta attactggcg gaggtcgtcg catcggcctc gccctcgcct
    17221 ggcatttcat taatcaaaag caaccggtga ttgtcagcta tcggacgcac tatccagcca
    17281 ttgatggact gattaaggca ggtgcgcagt gtattcaggc tgatttttcg accaacgacg
    17341 gtgtgatggc gtttgccgat gaagtactaa aaagcaccca tggtctgcgt gctattttgc
    17401 ataacgccag tgcgtggatg gcggaaaaac tgggtgcgcc actggccgac gtactggctt
    17461 gcatgatgca gatccacgtt aataccccat acctgctcaa ccatgcgctg gaaagattac
    17521 tgcgtgggca cggacacgcc gccagcgata tcattcactt taccgattat gtggtggagc
    17581 gcggtagcga caagcatatt gcgtatgctg caagcaaagc ggcactggat aatatgaccc
    17641 gctcgtttgc ccgcaagctg gcaccggaag tgaaagtgaa ttctattgct ccatcgctga
    17701 tcctgtttaa tgaacatgat gatgccgaat atcgacaaca ggcgctgaat aaatcactga
    17761 tgaaaaccgc gcctggcgag aaagaagtga tcgacctggt cgattactta cttaccagtt
    17821 gctttgtcac cggacgcagt ttcccacttg atggcggtcg tcatctgcgt taatgcagct
    17881 ttatccagca tataatcaac acccatgcaa cacgcaacag aaccaacaaa agcggcgggc
    17941 aagcgcatca ttccggtgaa ataccacagc gacaccaggt aaacaaaata gggaataatc
    18001 gaccacatac taaaaatgat ggttgcgcgt aaggcttcaa tgccgcgttc gctggcaaca
    18061 atataatgcg cgataagcgc aaaggtcggg aaaagtggaa tcagcccggc gatataataa
    18121 tttttcgttt ttgctaaaac accaatcaac agcactacca gcgcaccgag cgcggctttg
    18181 attttacctt aacacttcca taacaagtca tcagtagaat acctgatgaa aacttgttta
    18241 gaaacgattg atagtaagta aaaacagcgc ggtgtattgt gacgttttta tatctaccgt
    18301 gaatgttatg aacactatcg tatttgtgga agatgatgcg gaagtcggtt cactgattgc
    18361 cgcgtacctg gcaaaacatg atatgcaggt taccgtagag ccgcgcggcg accaggccga
    18421 agaaaccatt ttgcgagaaa atccggattt ggtgttactc gacatcatgc taccaggcaa
    18481 ggacggcatt accatttgtc gtgatttacg cgcaaagtgg tctggaccga ttgttcttct
    18541 aacctctctc gatagcgata tgaaccacat cctggcactg gaaatgggtg cctgcgacta
    18601 tattctcaaa acgatgcccc ctgctgtttt gctagcgcgt ttacgtttgc atttgcgtca
    18661 gaatgagcaa gccacactga ccaaaggtct tcaggaaacg tctctgactc cctacaaagc
    18721 cctgcatttc ggcacgttga ccatcgatcc catcaaccgc gtagtcaccc tggctaacac
    18781 tgaaatctcg ctctcgacag ctgatttcga attattgtgg gaattagcta cccatgccgg
    18841 gcaaatcatg gaccgcgatg cattgctgaa aaatttacgc ggcgtcagtt atgacggact
    18901 ggatcgtaac gtggacgtgg ctatttcgcg gttaagaaaa aaactgctcg ataacgccgc
    18961 agaaccttat cgcattaaaa ctgtgcgtaa caaaggctat ctttttgcgc ctcatgcatg
    19021 ggaataagcg atgaaaaaac tgtttatcca gttttacctg ttattgtttg tctgcttcct
    19081 tgtgatgtct ctgctggttg ggttggtgta caaatttacc gccgaacgcg cgggcaaaca
    19141 gtcgctggat gatttgatga acagttcgct gtatctgatg cgcagcgaat tgcgtgagat
    19201 ccccccacac gactggggta agacgctgaa agagatggat ttaaatctct ctttcgatct
    19261 gcgtgtcgag ccactgagta aataccatct tgatgatatt tccatgcacc gactgcgtgg
    19321 cggcgaaatt gtcgccctgg acgatcagta cacgtttttg cagcgtatcc cgcgcagcca
    19381 ctacgtgctg gcagttggtc ctgttcctta tctttattat ctccatcaga tgcgattgct
    19441 ggatatcgcc ctgatcgctt ttattgctat ttccctcgcc tttccggtgt ttatctggat
    19501 gcgtccgcac tggcaggata tgttaaaact ggaagcagcg gcgcaacgat ttggcgatgg
    19561 gcatctctat gagcgtatcc actttgatga gggttcgagc tttgaacgac ttggtatcgc
    19621 atttaaccag atggcggaca atatcaacgc cttgattgcc agcaaaaaac agcttattga
    19681 cggtatcgct cacgaactgc gaacaccgtt agtgcgcctg cgttatcgac tggagatgag
    19741 cgataacctg agcgccgccg agtcccaggc gttgaatcgt gatatcagtc aacttgaagc
    19801 tttaattgaa gagctgctga cttatgcccg actcgatcgc ccacaaaacg agcttcatct
    19861 tagcgaacca gacctgccgt tgtggctgtc aacgcatctg gcagatattc aggcagtaac
    19921 gcccgataaa acggtacgga taaaaacgct cgtgcaaggc cattatgcgg cgttggatat
    19981 gcgcttaatg gagcgcgtgc tggataattt gctcaataac gccctgcggt actgccattc
    20041 aacggttgaa accagcctgc tactgtcggg gaatagagcg acattaattg ttgaggatga
    20101 tggcccaggg attgccccag aaaaccgcga acatatcttt gaaccttttg ttcgcctcga
    20161 tcccagccgg gatcgctcaa ccggcggctg cgggctgggg ctggcaattg tccactctat
    20221 agcactggca atgggcggta cggttaattg tgacaccagc gaactgggtg gtgctcgctt
    20281 ctcgtttagc tggccgttat ggcataacat cccgcaattt acctctgcct gacactacgc
    20341 gcacgatggt caagtcacca ccattgtgct ataaattaag gatgttgtaa ctaaagtggt
    20401 taatattatg gcgcgttacg atctcgtaga ccgactcaac actacctttc gccagatgga
    20461 acaagagctg gctgcatttg ccgctcatct tgagcaacac aagctattgg ttgcccgcgt
    20521 gttctctttg ccggaggtaa aaaaagagga tgagcataat ccgcttaatc gtattgaggt
    20581 gaaacaacat ctcggcaacg acgcgcagtc gctggcgttg cgtcatttcc gccatttatt
    20641 tattcaacaa cagtccgaaa atcgcagcag caaagccgct gtccgtctgc ctggcgtgtt
    20701 gtgttaccag gtcgataacc tttcgcaagc agcgttggtc agtcatattc agcacatcaa
    20761 taaactcaag accacgttcg agcatatcgt cacggttgag tcagaactcc ccaccgcggc
    20821 acgttttgaa tgggtgcatc gtcatttgcc ggggctgatc acccttaatg cttaccgctc
    20881 gctcaccgtt ctgcacgacc ccgccacttt acgttttggc tgggctaata aacatatcat
    20941 taaaaatttg cattgtgatg aagtcctggc acagctggaa aaaagcctga aatcaccacg
    21001 cagtgtcgca ccgtggacgc gcgaggagtg gcaaagaaaa ctggagcgag agtatcagga
    21061 tatcgctgcc ctgccacaga acgcgaagtt aaaaatcaaa cgtccggtga aggtgcagcc
    21121 gattgcccgc gtctggtaca aaggagatca aaaacaagtg caacacgcct gccctacacc
    21181 gctgattgca ctgattaatc gggataatgg tgcgggcgtg ccggacgttg gtgagttgtt
    21241 aaattacgat gccgacaatg tgcagcaccg ttataaacct caggcgcagc cgcttcgttt
    21301 gatcattcca cggctgcacc tgtatgttgc agattaacgc ccggctttca tactgccgac
    21361 catctgttct ggccgtaccc agctgtcaaa ctcggcttcg ctaagatacc ccagcgcaag
    21421 ggccgcagct tttaaggtca gcccttcttt atgcgctttt ttggcgatct ccgcggcttt
    21481 gtcataacca atgtgggtgt taagcgcagt caccagcatc agcgattcat tgagtaattg
    21541 attgattcgc tcacgattcg gttcaatacc cactgcgcag tgtttgttaa aactttccat
    21601 gccatctgcc agcaagcgca ccgattgcag gaaattatgg atcaccatcg gacggaagac
    21661 gttcagttca aagttaccgg aagcgccacc catgttgatc gccacgtcgt tccccattac
    21721 ctgacagcag agcatggtta atgcttcaca ctgggttggg ttcactttcc ccggcatgat
    21781 tgagctgccc ggctcatttt ccgggattga gatttcacca attccgcagc gcgggccaga
    21841 ggccagccag cggacatcat tggcgatttt catcagtgac gcagccaacc ctttcaacgc
    21901 gccgtgcgcc tgaaccaggg catcacaggt cgcaagcgct tcaaatttgt tcggcgcggt
    21961 aacaaacggt gcacaggtaa tgactgccag ttcatctgct acgcgacgcg catactccgg
    22021 atgggtattt agtccagtac ccaccgctgt accgccaaga gccagttccg ctacgtgagg
    22081 caggctgtat tcgatatgtt tgagattatg ctcgagcatc gctacccagc cggaaatctc
    22141 ctgccctagt gttagcggcg tggcgtcctg caagtgggtt cgaccgattt tgacgatatc
    22201 ggcaaatgca cgcgattttt cactcagtgt ctgcgtcagg gttttaagct gcggaatgag
    22261 ttgcttgcgc agcgccagca gcgccgcaac gtgcatcgcc gtcggaaaga catcgttgga
    22321 actttggctt ttgttcacgt cgtcgttagg gtgaacttta cgttccatcc cgcgcacgcc
    22381 gccgagtaat tcactggccc ggttagccag cacttcgttc atgttcatgt tactttgcgt
    22441 gccggagccg gtctgccaga tagccagcgg gaattcgtcg tcatgctgtc ctgccagtac
    22501 ttcatccgcc gcctgccgaa tggcgctcgc tttctcttca gacaacaagc ctaaatcttc
    22561 attaactttt gccgctgcgc gcttggttag cgccagcgca tgaatcagtg aggtgggcat
    22621 tttctccgtc gaaatgcgga aatgctccag cgagcgttga gtttgtgcg
//
LOCUS       DAJTYD010000052        22609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTYD010000052 DAJTYD010000000
VERSION     DAJTYD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.052"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(40..1689)
                     /gene="actP"
                     /locus_tag="OO585_001808"
     CDS             complement(40..1689)
                     /gene="actP"
                     /locus_tag="OO585_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6629422.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1686..2000)
                     /locus_tag="OO585_001809"
     CDS             complement(1686..2000)
                     /locus_tag="OO585_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6629423.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2132..4091)
                     /gene="acs"
                     /locus_tag="OO585_001810"
                     /pseudo
     CDS             complement(2132..4091)
                     /gene="acs"
                     /locus_tag="OO585_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4484..5920
                     /gene="nrfA"
                     /locus_tag="OO585_001811"
     CDS             4484..5920
                     /gene="nrfA"
                     /locus_tag="OO585_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6629424.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5965..6531
                     /gene="nrfB"
                     /locus_tag="OO585_001812"
     CDS             5965..6531
                     /gene="nrfB"
                     /locus_tag="OO585_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6629425.1"
                     /translation="MSVLRSLLTARVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6528..7199
                     /gene="nrfC"
                     /locus_tag="OO585_001813"
     CDS             6528..7199
                     /gene="nrfC"
                     /locus_tag="OO585_001813"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6629426.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7196..8152
                     /gene="nrfD"
                     /locus_tag="OO585_001814"
     CDS             7196..8152
                     /gene="nrfD"
                     /locus_tag="OO585_001814"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6629427.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8172..9890
                     /locus_tag="OO585_001815"
     CDS             8172..9890
                     /locus_tag="OO585_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6629428.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9883..10266
                     /gene="nrfF"
                     /locus_tag="OO585_001816"
     CDS             9883..10266
                     /gene="nrfF"
                     /locus_tag="OO585_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6629429.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10263..10859
                     /gene="nrfG"
                     /locus_tag="OO585_001817"
     CDS             10263..10859
                     /gene="nrfG"
                     /locus_tag="OO585_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6629430.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYVALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11200..12513
                     /gene="gltP"
                     /locus_tag="OO585_001818"
     CDS             11200..12513
                     /gene="gltP"
                     /locus_tag="OO585_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6629431.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12704..13392)
                     /locus_tag="OO585_001819"
                     /pseudo
     CDS             complement(12704..13392)
                     /locus_tag="OO585_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13486..15633)
                     /gene="fdhF"
                     /locus_tag="OO585_001820"
     CDS             complement(13486..15633)
                     /gene="fdhF"
                     /locus_tag="OO585_001820"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15214..15216),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6629432.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15831..17297)
                     /gene="mdtP"
                     /locus_tag="OO585_001821"
     CDS             complement(15831..17297)
                     /gene="mdtP"
                     /locus_tag="OO585_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6629433.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGIAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17294..19345)
                     /gene="mdtO"
                     /locus_tag="OO585_001822"
     CDS             complement(17294..19345)
                     /gene="mdtO"
                     /locus_tag="OO585_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6629434.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19345..20376)
                     /gene="mdtN"
                     /locus_tag="OO585_001823"
     CDS             complement(19345..20376)
                     /gene="mdtN"
                     /locus_tag="OO585_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6629435.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20395..20670)
                     /locus_tag="OO585_001824"
     CDS             complement(20395..20670)
                     /locus_tag="OO585_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629436.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20879..>22609)
                     /gene="yjcS"
                     /locus_tag="OO585_001825"
     CDS             complement(20879..>22609)
                     /gene="yjcS"
                     /locus_tag="OO585_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6629437.1"
                     /translation="RGEDYKFDINAPAPETVNPSLWRQSQLNGISGLFKVTDRMYQVR
                     GQDISNITFIEGDTGIIVIDPLVTPPSAKAALDLYFQNRPQKPIVAVIYTHSHTDHYG
                     GVKGIISEADVKSGKVQVIAPAGFMDEAISENVLAGNIMSHRALYSYGLLLAHNPQGN
                     IGNGLGVTLASGYPSIIAPNKTITKTGEKMIIDGLEFDFLMTPGSEAPAEMHFYIPAL
                     KALCTAENATHTLHNFYTLRGAKTRDTSKWTEYLNETLDMWGNDAEVLFMPHTWPVRG
                     NKHINDYIGKYRDTIKYIHDQTLHLANQGYTMNEIGDMIKLPPALANNWASRGYYGSV
                     SHNARAVYNFYLGYYDGNPANLHPYGQVEMGKRYVQALGGSARVISLAQEANKQGDYR
                     WSAELLKQVIAANPGDQVAKNLQANNFEQLGYQAESATWRGFYLTGAKELREGVHKFS
                     HGTTGSPDTIRGMSVEMLFDFMSVRLDSAKAAGKNISLNFNMSNGDNLNLTLNDSVLN
                     YRKTLQPQADASFYISREDLHAVLTGQAKMADLVKAKKAKIIGNGAKLEEIIACLDNF
                     DLWVNIVTPN"
BASE COUNT         5071 a         5960 c         6190 g         5388 t
ORIGIN      
        1 agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc ggccttgctc
       61 aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac gctcgcgcgc
      121 accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg ccacgctgat
      181 agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga ggatctgtac
      241 ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca gccagccgcc
      301 cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa tgatcgggaa
      361 gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt tctgattctc
      421 aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga ttttcgatac
      481 ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca agtcatgcga
      541 aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag tggcgaaagc
      601 aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca ggtgaacggc
      661 cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg gattcgcacc
      721 aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt agcccataaa
      781 cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg taaagaagcg
      841 catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca gagagagcgc
      901 ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt tcgggtgtac
      961 cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca tcaccataaa
     1021 ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca cccaggtggt
     1081 cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca gcaccaccgc
     1141 aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta ccatctgggc
     1201 gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac gaatcggccc
     1261 ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt tacgcagacg
     1321 ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg agtaaattaa
     1381 gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg cggcggacat
     1441 atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc ctgccgtgta
     1501 gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga gcgtaaacac
     1561 gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct ctacggcccc
     1621 gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg ccgtcagaac
     1681 tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc gaattcgccg
     1741 ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac tccaacacca
     1801 attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag ccagccgggc
     1861 gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat cgacaggatg
     1921 gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc ttctatccgc
     1981 tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt gttttaattc
     2041 ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt aggccggata
     2101 aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata gcctgcttct
     2161 cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg cccaggttgc
     2221 tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg gagcgggttt
     2281 taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca atctctttac
     2341 gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg tgattaagcg
     2401 tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact acggcggctt
     2461 cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc agacggtgac
     2521 cggagacgtt cagcacgtcg tccacatgcc cggtgatcca gtaatagcca tcttcatcgc
     2581 gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag gtctgttcaa
     2641 aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg gtgatcacca
     2701 ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc gccggttgca
     2761 cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca ggcagtggcg
     2821 tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga catttctcgt
     2881 tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg cccacggaac
     2941 cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct tccgccatca
     3001 gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg tccaccacct
     3061 gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc gtggtcgcac
     3121 cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc acatcggcgg
     3181 tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc agcgccgcgt
     3241 acaccagata accgccggta gtatgcagca caccttttgg cttaccggta gaaccggagg
     3301 tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc tgatcgctcg
     3361 cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca attttcccgc
     3421 cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt ttcagcgcgt
     3481 catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc gtcggaagtg
     3541 atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg cgagaagccg
     3601 ccgaaaatca ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc aaccgcggct
     3661 tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc cagctcgagc
     3721 agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat atgtttgctc
     3781 tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc ttgcagatgg
     3841 cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt aatggacaca
     3901 ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca gtcaagaatt
     3961 tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata cattgcctcg
     4021 tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt gtgtttgtga
     4081 atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca taagcgttaa
     4141 atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca aaagtagaat
     4201 tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga ttaattcttt
     4261 gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta actctaaagt
     4321 ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg ttatttgtta
     4381 cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg aaaaacaata
     4441 aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat
     4501 aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt ctgttcacgc
     4561 tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg aaacctttgc
     4621 cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt cagagcgtgt
     4681 tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc ccttctcgcg
     4741 cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg aaaccctgcg
     4801 taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct ggagttgtaa
     4861 aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact tccacggtaa
     4921 gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact gccataacac
     4981 cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc
     5041 tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc
     5101 gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca aagcggttaa
     5161 attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg acaaaattgc
     5221 cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga
     5281 atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta tcgactgcca
     5341 tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa ttggtaatcc
     5401 gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag ctgccttgca
     5461 aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg ttgaagatca
     5521 actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga cggaagctga
     5581 aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc tggcgatcgc
     5641 ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg gtacggcgat
     5701 ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca aaggcatcac
     5761 ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg ccattggcct
     5821 gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga tcccgcagtg
     5881 ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg cctcgcaagg
     5941 ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac tgctagggtg
     6001 ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc ggcgcaggca
     6061 tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg tctggactgt
     6121 cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa cccgaataac
     6181 aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca ccgcgaaggg
     6241 gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca gaacagcgtc
     6301 tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca cgatgtccac
     6361 gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga tacgatgcag
     6421 acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga tcagcgcacc
     6481 aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg acctggtctc
     6541 gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc gcagggcgcg
     6601 tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat gacgagtcgt
     6661 tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag gtgccggaag
     6721 gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct gatgtgaaat
     6781 atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc
     6841 cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat cctgatctct
     6901 gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg
     6961 tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag gccggtaagc
     7021 tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat ctggacgatc
     7081 ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac aagctggcgc
     7141 tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg agccaatgac
     7201 gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg ccatctacct
     7261 gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac gtcgcttcta
     7321 cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg tcgggccggg
     7381 cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt ggaccttctg
     7441 gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga tgctgtttca
     7501 gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc
     7561 ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga gcctgctgac
     7621 gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc tgggggctta
     7681 taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc cgatcctgcc
     7741 ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc
     7801 gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc ggatggaaat
     7861 ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg
     7921 cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct ggacgtggtg
     7981 gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca aatcgtgggt
     8041 caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc tggtcggcgt
     8101 gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt aagccagaaa
     8161 agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg ttgttaagtc
     8221 tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg cgctggcctg
     8281 ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg ctgctcgcct
     8341 ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat gtcgcccaac
     8401 atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc ggtcatgaag
     8461 gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt
     8521 atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct ctcatgctcg
     8581 ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata tttccaccag
     8641 caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc tttcatccac
     8701 cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg ctggcgagtc
     8761 tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg gcgttacctg
     8821 gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac tgcgaactcg
     8881 gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta ccctggcttt
     8941 ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt tttcgccact
     9001 ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc ttaattgtcc
     9061 gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc gccgtgccgt
     9121 tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc tggcgagcgc
     9181 gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta atcctcgcta
     9241 cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt tatccgatga
     9301 tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga
     9361 cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt gtctctggca
     9421 aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt gccgctggga
     9481 tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg ggtcagcagg
     9541 tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc aaaggcaatt
     9601 acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt ggtgaactga
     9661 tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg tcaattcgct
     9721 ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg gatcgttacg
     9781 cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga ttgttgatga
     9841 ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa aggacttctc
     9901 acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac ctggcaattc
     9961 gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg ttgtccgcag
    10021 tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat gcgccatcag
    10081 gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg gatgaccgaa
    10141 cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct ggtgttgtgg
    10201 gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt gagggcgaag
    10261 cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt ttgatggtat
    10321 ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat gcggaatatc
    10381 agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa gcgcagcttc
    10441 aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg gcgttactgg
    10501 gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac cgtcaggcgt
    10561 tgcaactacg tggtgagaac gctgaactgt atgtggcgct ggcgacggtg ctttattacc
    10621 aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc ctcgcgctgg
    10681 actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg caggcgaatt
    10741 acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc cggatcaacc
    10801 ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga tcggattaat
    10861 catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat gaacatatgc
    10921 caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct ttccataaca
    10981 aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg cagcgaaaag
    11041 ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat aagcgttaaa
    11101 aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt tctcgcgttt
    11161 ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat aaaattcagc
    11221 ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag ctacctgcac
    11281 taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc gggtgatatc
    11341 ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct ggtggtgggt
    11401 atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac cattatttac
    11461 ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa cgtcttccag
    11521 cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa atatcagagc
    11581 actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt gtcgctggtg
    11641 ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat cttcttctcg
    11701 gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc gctggtgacc
    11761 gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat gcgttatgca
    11821 ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt ctcttctctg
    11881 tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt cgcgctggta
    11941 gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat tcgtattctg
    12001 aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact gccgcgaatt
    12061 attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt ggtgccgacc
    12121 ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc tattttcatc
    12181 gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt gctgacgctg
    12241 atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt gttgctggca
    12301 acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg tgttgaccgt
    12361 atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc ggtgctggtg
    12421 attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga gcgtgaagtg
    12481 ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt tatccacccg
    12541 gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg ccagacagcg
    12601 caatagcctg atttagcgtg attttgtagg tcggataagg cgtttacgcc gcatccgaca
    12661 tcaacgcctg atgcgacgct taacgcgtct tatcaggcct accttaaccg ttggttaatt
    12721 tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc aaccagtgca
    12781 gcgccttttg tttgttcttc tcgataaaac cttcttcacc gtttaagaac atcattcccg
    12841 cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa tactaggttg
    12901 ccttgtcatc gtcggcctta atacccacgc cgttggcgta aatcaaacca agcagcattt
    12961 gggcatcgac ggcagagtcg ttctccagat cttccgaggc ttttctaaca gcgaaatcgc
    13021 ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca gcgttacttc
    13081 accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt gtggatagtc
    13141 cagactgccc ggattggtga ttttgatctg cgccaacagc gcgcaggcat cagcatcacc
    13201 gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg agctaaacca
    13261 gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct ttaaatactg
    13321 gctgccaggc tcggagtcgt tggcgtgggc aaagaatgtc aaaaacaaca tcaatgcgat
    13381 aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa gagttcgaat
    13441 ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg ccagtgccgc
    13501 ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct cggcggcgcg
    13561 ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg ttttcgtaat
    13621 cgggcttaag ttttcggtaa ccagctcgtt acaggcacca atccaccact ggtaggtcat
    13681 gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa ttttgccttt
    13741 acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg cgtcttcggt
    13801 attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac agttaccggt
    13861 catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca tcgggtactc
    13921 gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg ccagaccgtt
    13981 cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat ctgaagtatc
    14041 gcggcaaggc cactgaatga agccctgttc gcccattttc tcgtaagtcg caccgtagaa
    14101 atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt agtgcatcgg
    14161 ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca gatcccattt
    14221 cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa acacgccttc
    14281 atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg ttttggtcat
    14341 aaagatgtcc tgaacgatga ccagttccag atcttcaaag gctttacgta ctgccgacag
    14401 ctccgcatca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca cttcgccatg
    14461 cgctgcgcgg tgcggcagct cgctgatgcg atagccggta tgagcaggca ggctttccac
    14521 gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact ggtatcccgg
    14581 atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct gaccacgaac
    14641 cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga ggctggtcag
    14701 agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc acaggatggc
    14761 ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg cgctgacgcc
    14821 ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat actcttcaaa
    14881 gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa taatgacatg
    14941 gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt gcatgtcagc
    15001 aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc cgttacgttt
    15061 agcgttaatt acgtgattcg ccatgattgg gtgggaatcc gccgggttgt acccgaaaac
    15121 gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc cattaccgac
    15181 cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc agtcaacgtt
    15241 attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt cgttacccgt
    15301 accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt tgatggcgct
    15361 caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga gtttgccgcc
    15421 acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg tatcgttaat
    15481 gaagtcccag ccataataac ccttcagaca cagggtaccc tggttggttt tcccctgcgc
    15541 tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc aacctgatgc
    15601 gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta atcaaatcac
    15661 gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt ggggtcattc
    15721 cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg tcgtcagttt
    15781 tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt ttattttttc
    15841 tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg aatgctgtgg
    15901 atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagcac tggcaaccgg
    15961 gcttcggtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc ggcagcgcgc
    16021 tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgttcgtc gttgagcgtt
    16081 tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga ctggttgtaa
    16141 cgttcaatca tcatgttgct ggcggcgcga gtgccttcga gattggcatt caaccgtcca
    16201 ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact ggtttttttg
    16261 aataaggtat ccaggtggat ggagtccaga ccgaaaaacg ctttgatatc aaagctcggg
    16321 tagaacagtg cccgcgcgga atccacctga tctaatgacg cctgaacata ccagcgcatg
    16381 gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg aatgccggtc
    16441 tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc cgcgccaatc
    16501 aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc aatttgttta
    16561 tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc cagaccgtgc
    16621 gccactttac tctggtgcgc tttcaccgcg taatcaatca catcgcgagt ttgttctaac
    16681 agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctat gcccgtggtc
    16741 agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc aatggcggcg
    16801 gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa caaacctact
    16861 gtggcttccg tatagtacgg cccgtccata cccagtgagg gcgcatccat cgcataaggg
    16921 cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc tgcgacctgt
    16981 aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc ccgcagtttc
    17041 gcttcggcga gggtgtgtga accacttagc gtccgttgga tcagcgcatc cagctgcggg
    17101 tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag atgaatatcg
    17161 tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc cttacgtacc
    17221 agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag cagacgtgaa
    17281 agttgacgat tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc gttaatgccg
    17341 gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc tgttttaata
    17401 aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga ggagaacagc
    17461 tgagtgcggt tgccagaccg gcagcgtatt tttccagggc gtcggcaaac tgacctgctc
    17521 tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca tcccaggcgt
    17581 gaagaagatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt aaggcgcgtt
    17641 cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca caggcattaa
    17701 acgccgcatg cagacctata cgaatttgca gataagtgcg cagagccgtg acttcctgct
    17761 ggcgtgggat ccgcattact ttacttagca tacccagcgc accggctagt ttttgcggca
    17821 gagtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac accacggtac
    17881 caatgatgat acccagggcg cgatcgcgaa tttccaccag gtcgtacact gggccaaaaa
    17941 cgttttcgag cgtggcgagc gcgaaggtga cgaccatctg tgtgccgata taagaagagc
    18001 gttcagagct ggtggcaatc catgcgccca acaggaaaat cggtgccagc acaaacagca
    18061 attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc gccagaatcg
    18121 cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa ccgacatttg
    18181 gattagcgac gatcacgcag gtcagcatac aggtgtgaat gccttcccag tccacgccgc
    18241 tgtaaaaggt gtaacagatc aaacaggcga gcaatgtttt taccgcgtag cgtatatagt
    18301 ctggattggt aaaagcatcg gcgaccattg atggcggttt ggcggcgggc gttggcggcg
    18361 tattcgggtt catctggccc agttgtaaca acgtctggca gatattctcc aggttacatt
    18421 cccgtgccgc caccgcttca ctttcactga tccgccagtc gctttgccag cactgacctt
    18481 cagcaacggc atgctgcagc ttgttgattt ctgaggctaa tttttgccga aattcaataa
    18541 ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag taaatgtagg
    18601 ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg gcatcgtccg
    18661 cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc cgcgtttcgg
    18721 gtagcggtgc gaggctgtct gtcaggtgac taatggcatc atcaagccga tcattaagcg
    18781 cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt aacgtcatca
    18841 gcaaggttgg atagaggcca acaacgatac accacagcgt taagcgcacg accacttccg
    18901 gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg acagcgaaaa
    18961 agaccagtcc cagccgatgg gtgcgcatca aaaacatgca gcccatcagg atcggcccgg
    19021 cgatgatcaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc
    19081 cgatctccag caccgtggca accacaaaca agatcgccac aaatttggtg taaaacgcgt
    19141 tcgactgaat accgtaaaac agcactgcca gcgataacgc cacaaaaggg atctcaaaag
    19201 tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacgatc tgcggcacgc
    19261 ggccgggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta
    19321 atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc
    19381 gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc
    19441 aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca ccaggccgcc
    19501 gtcattcggt agcacaccgt agccaatcga atccacttta ccctcgaagg ttttgccgct
    19561 gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc
    19621 gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa tagggcgcat
    19681 ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat caaacggcgc
    19741 gcgaacggtc gccatttcca gatgcagttt ggtcagggca atatccgctt cgaccgccgc
    19801 acgctgggca actaatacat ccacgccgct gacggcgctg gcggctgact gcgcctgtaa
    19861 caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac ggtcaacatc
    19921 ttccgctgag acaaaacctt ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc
    19981 ctgtttcgcg gcggcacgtg ctttttctac cgtggcatta accgagtcgg caccaaactg
    20041 ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc
    20101 ttcagctttc gccagattgg cttcgtacgg gcgcgggtca atgcggaaca gcaaatcgcc
    20161 ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcggccgc tgacttccgg
    20221 caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg cactgtctac
    20281 gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca gagcagggaa
    20341 tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa
    20401 aaatgccagc cagaacagca tggcgtagac agcaaaaagg gcggtataaa taattccgac
    20461 aaatgccaga ttgatatttg cccgctgaat aattcgcctc gtgattagcg tcaaaataag
    20521 gctggcaatg gcgcagaaaa accagctggg ataataagcg ccgatcaccg ggattgccgg
    20581 agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt ttttgagttg
    20641 catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtttgc tgcgaacaat
    20701 atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc ttgatgaata
    20761 aaaatagctt tattcagcca ccgcattcat aacttatata ttttatgtgt taattgattg
    20821 tatgtatttt tataataaat gcttttgggg gcttaagcgc ccccaaaagt gagtggcatt
    20881 aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggca ataatttctt
    20941 ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga tccgccattt
    21001 tggcttgtcc tgtcagcacg gcgtgcagat cttcacggct gatgtagaaa gaggcgtcgg
    21061 cttgcggttg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc aggttgaggt
    21121 tatcgccgtt gctcatattg aagttcaggc tgatattttt acccgcagct ttcgcgctat
    21181 cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga atggtgtccg
    21241 gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct ttcgcgccgg
    21301 tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt tcaaagttat
    21361 tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc tgtttcagca
    21421 gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg ctgatgacac
    21481 gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga ccatacggat
    21541 gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc gcgcgggcgt
    21601 tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt gcaggcggca
    21661 gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg tgcagggtct
    21721 ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg atatgcttat
    21781 tgccccggac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta ccccacatat
    21841 ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc gcgccgcgca
    21901 gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc gctttcaggg
    21961 ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc aggaagtcaa
    22021 actccaggcc gtcgataatc atcttctcac cggttttggt gatggttttg ttcggtgcga
    22081 tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg ttaccctgag
    22141 ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gtggctcata atattacccg
    22201 ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata acctgcactt
    22261 taccggattt cacatcagct tcagagataa tgcctttcac gccgccatag tggtcggtat
    22321 ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg aagtaaaggt
    22381 caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata atcccggtat
    22441 caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac attctgtcgg
    22501 tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg ctagggttaa
    22561 cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccgcg
//
LOCUS       DAJTYD010000053        22018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTYD010000053 DAJTYD010000000
VERSION     DAJTYD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.053"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..990
                     /locus_tag="OO585_001826"
     CDS             <1..990
                     /locus_tag="OO585_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6629438.1"
                     /translation="GLGTDKGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGI
                     RLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALIL
                     DEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMED
                     WQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALP
                     LTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGI
                     IPPAGKTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1170..1964
                     /gene="potC"
                     /locus_tag="OO585_001827"
     CDS             1170..1964
                     /gene="potC"
                     /locus_tag="OO585_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6629439.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDETKGNTGDVK"
     gene            1961..3007
                     /gene="potD"
                     /locus_tag="OO585_001828"
     CDS             1961..3007
                     /gene="potD"
                     /locus_tag="OO585_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6629440.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3163..3984)
                     /gene="cobB"
                     /locus_tag="OO585_001829"
     CDS             complement(3163..3984)
                     /gene="cobB"
                     /locus_tag="OO585_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6629441.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQEALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4000..4911)
                     /gene="nagK"
                     /locus_tag="OO585_001830"
     CDS             complement(4000..4911)
                     /gene="nagK"
                     /locus_tag="OO585_001830"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6629442.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(4940..6184)
                     /gene="lolE"
                     /locus_tag="OO585_001831"
     CDS             complement(4940..6184)
                     /gene="lolE"
                     /locus_tag="OO585_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6629443.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6184..6885)
                     /gene="lolD"
                     /locus_tag="OO585_001832"
     CDS             complement(6184..6885)
                     /gene="lolD"
                     /locus_tag="OO585_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6629444.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(6878..8077)
                     /gene="lolC"
                     /locus_tag="OO585_001833"
     CDS             complement(6878..8077)
                     /gene="lolC"
                     /locus_tag="OO585_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6629445.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8339..9412
                     /locus_tag="OO585_001834"
     CDS             8339..9412
                     /locus_tag="OO585_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6629446.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9540..12986
                     /gene="mfd"
                     /locus_tag="OO585_001835"
     CDS             9540..12986
                     /gene="mfd"
                     /locus_tag="OO585_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6629447.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13130..14092
                     /gene="ldtC"
                     /locus_tag="OO585_001836"
     CDS             13130..14092
                     /gene="ldtC"
                     /locus_tag="OO585_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6629448.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14175..14432)
                     /gene="bhsA"
                     /locus_tag="OO585_001837"
     CDS             complement(14175..14432)
                     /gene="bhsA"
                     /locus_tag="OO585_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6629449.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14673..15305
                     /gene="comR"
                     /locus_tag="OO585_001838"
     CDS             14673..15305
                     /gene="comR"
                     /locus_tag="OO585_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6629450.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15367..15906)
                     /locus_tag="OO585_001839"
     CDS             complement(15367..15906)
                     /locus_tag="OO585_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6629451.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16133..17437)
                     /gene="ndh"
                     /locus_tag="OO585_001840"
     CDS             complement(16133..17437)
                     /gene="ndh"
                     /locus_tag="OO585_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6629452.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(17837..18379)
                     /locus_tag="OO585_001841"
     CDS             complement(17837..18379)
                     /locus_tag="OO585_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6629453.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18402..19427)
                     /gene="nagZ"
                     /locus_tag="OO585_001842"
     CDS             complement(18402..19427)
                     /gene="nagZ"
                     /locus_tag="OO585_001842"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6629454.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19438..20262)
                     /gene="thiK"
                     /locus_tag="OO585_001843"
     CDS             complement(19438..20262)
                     /gene="thiK"
                     /locus_tag="OO585_001843"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6629455.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20246..20884)
                     /gene="lpoB"
                     /locus_tag="OO585_001844"
     CDS             complement(20246..20884)
                     /gene="lpoB"
                     /locus_tag="OO585_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6629456.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(20898..21275)
                     /locus_tag="OO585_001845"
     CDS             complement(20898..21275)
                     /locus_tag="OO585_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6629457.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21278..21637)
                     /gene="hinT"
                     /locus_tag="OO585_001846"
     CDS             complement(21278..21637)
                     /gene="hinT"
                     /locus_tag="OO585_001846"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6629458.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5463 a         5794 c         5469 g         5292 t
ORIGIN      
        1 ggactcggaa cagacaaagg taaggcagaa aaaatcgctt ccacagccaa tcagcgaata
       61 gctgcagcag aagctgaata tttcatgcgc aaaattgatg aaagtccgtc agcaacaaaa
      121 cgtcggggta tcagattaaa ggcatgggtt gatcgatatc tgaaaataca ggacacgcga
      181 ctgaaaaatg gagatattgc agctacaact cacaaagaaa aaactcgaat ggctgcatac
      241 ctggtttccc gtctgggaaa ccacccattg aaagaactgg aagtaagaga ctttgcatta
      301 atactggatg agtggctgga taaagacatg gtcagcacag cgagagtaaa tcgtggatta
      361 tgggttgata tttataaaga agcacagcat gcaggggaag ttcctcctgg atggaatcct
      421 ccggaggcta cccgtaaacc gatccctaaa gtaaccagag ccaggctcac catggaagac
      481 tggcaaaaaa tttacaatgc aacgcctgaa aaacacttta tccgtaacgc aatgcttctt
      541 gcgattgtta ctggtcagcg ccgtgatgac atttgccaca tgcgtttttc agatgtgtgg
      601 aacgaacact tgcatatcac ccagggaaaa accggaatgc gtctggcgtt accgcttaca
      661 ctacgctgtg atgccattgg gataacgtta aaagaagtta ttgatgggtg ccgagacaga
      721 atattaagtc catatctaat ccatagtcgg caccagaaac aaccgaagcc gatgagtaaa
      781 gacaacctga gcgactactt tgccaaagca cgggatctgg ctgggataat tccaccagca
      841 ggaaaaactc cgccaacatt tcatgaacaa cgctctctat cagaacggct gtaccgtgca
      901 cagggtatcg atacaaaaac attactagga cataaagtcc aggcaaccac cgatcgctat
      961 aacgatactc gaggtcagga atgggtttaa gctggttatt tgacaaacac catatggaat
     1021 atccgtcacc atctcaggaa aaaagtgttg taaaatgcgt ggcctagttt tggggaaacg
     1081 ttttggagag attttggaga aaggaaaaaa ttgaataaat tcaaaatcct gaaagtgctc
     1141 caccttatga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
     1201 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
     1261 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
     1321 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
     1381 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
     1441 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatatcacca gatatcgtga
     1501 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
     1561 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
     1621 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
     1681 caattctgcg aaaaatcata ctgccactgg caatgccagc ggtggcagcg ggctgggtgt
     1741 taagctttac cctgtcgatg gacgatgtgg tagtttcttc gttcgtcacc ggaccaagtt
     1801 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
     1861 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agtcagctta
     1921 ttgctcgtga tgaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
     1981 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
     2041 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
     2101 caaagaaacc tgtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
     2161 caagctgaaa acgtacaaag acggtgcgta tgacctggtg gttccttcaa cctattacgt
     2221 cgataaaatg cgtaaagaag ggatgatcca gaagatcgac aagtcgaagt taacgaactt
     2281 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
     2341 tccgtatatc tggggggcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
     2401 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
     2461 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctattccg gtaacaccac
     2521 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
     2581 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
     2641 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
     2701 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
     2761 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
     2821 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
     2881 agtggcgaac gataaaacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
     2941 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
     3001 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttatttag caaggatgtg
     3061 gctaatgagt caggacagta gagttttatc cgactctttc gtcggtattg tggtccgctc
     3121 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc ccttcagcaa
     3181 cttttcaaca aactcaggca ccacctggct tgccgggccg taatatttct cggcaaattc
     3241 attaccgacc tgacttggtt cgagattcag ttccacggtg tgcgcgccat gcagtttcgc
     3301 ttcgtgaaca aacccagccg ccggataaac atgcccggaa gtaccaatgg caatgaaaat
     3361 atcagccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
     3421 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
     3481 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
     3541 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
     3601 cgttaccagc aaaaagcgat cgccgagagc ctcttgcagt ttagccagcg caagatgcgc
     3661 ggcgttaggc tgaatttttg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
     3721 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
     3781 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
     3841 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
     3901 aaaaatacgc tgacgcaaac gctcgcgcag gcggcgttta tttttacgaa aacgacttaa
     3961 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
     4021 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg
     4081 agctacaggt aagagatgac gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa
     4141 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
     4201 cagacaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
     4261 ttgattataa agcgcaataa tttcgggagc ctgcaactgt tgatgataat agtgttgata
     4321 cagccacgca aaaccgcgac cagacagata attctcaatg cagccatgct gaccacagcc
     4381 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
     4441 atggccaaac tcgccggtaa tgtagctttt accggtaatc ggtttgccgt tgaaaatcag
     4501 cccaccgcca acgccggtac caagaatcaa ccccatcacc agtggatatt gcgtaaattc
     4561 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
     4621 atcaagacgt gcgctcaggt cggcacgcag cggtttaccg ctggcagcgg ggacattggc
     4681 ggcatacagc gtaccatctt ctgtttccgg cattcccggt ataccaatgc cgacagaacc
     4741 tctacagcca aaacgttgat cagcttcggc taccagctcg cacactgcat ctaaaaatgc
     4801 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
     4861 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
     4921 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
     4981 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
     5041 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaatag atatcgctgg
     5101 agaggaactg atgaccgatc agcttttcaa tccgctcaat aatcggggta agttgcagtg
     5161 aaacaactac gccgataatc agcccacaca ggctgccgaa cagccctgcc agcaatccat
     5221 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
     5281 tatcgccact cttatctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
     5341 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
     5401 acatatagcc gtaagtacca atccagcttt taatataaac atagctgttg gtcatttcac
     5461 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
     5521 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
     5581 tgtgatcgag ttggccactc aactgcaaaa tacccgcaac gtgcaaacgc acacgttttg
     5641 gctgcatcag tttatgctca ggattcgagt tggggatcat gatcgacacc caatcgccct
     5701 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
     5761 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
     5821 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagcc
     5881 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc actttatcca
     5941 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcacca tgcggcacca
     6001 ctgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
     6061 tcaataccgc cacaccgagg gcaatgccaa tggtagaaat gacggagatc agcgacacca
     6121 tgccgccgcg ccgccgtccg cgactaaaac gcaggccaat taataacgat aaaggcatcg
     6181 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttctaa
     6241 ctggcggctc atacgtttcg ccagttgcag gtcgtgagta accaccagga aggcggtgcc
     6301 ctgcaagcga ttcaattccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
     6361 gttaccggta ggctcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
     6421 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
     6481 cagccccacc gcttttaaca tctccagtgc acggctgttg atttcagcgg gctttttctt
     6541 gccaatcagc agcggcatag ccacgttttc cagggcggta aaatccggca gcaggtggtg
     6601 aaactgataa ataaagccca gtttctggtt gcgcagttct gcttttgctg ccgacgacag
     6661 tttgctcatc ggttgaccgt taaagatcac atcgccggag gttggcgtat ccagtccgcc
     6721 cagcaggtgc agcaaggtgc ttttaccgga accagagcta ccgacgatcg ccatcatttc
     6781 accttcgccg acgctgaaac tgacattgtg cagtacatcg gtttgcacac tgccttcctg
     6841 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
     6901 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
     6961 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccatcggc agcgccgcgc
     7021 catcaagcag gacgccgatt attggcatca gattatttaa ctggctggca agcagtgcgc
     7081 caagcgccgc tccgaggatc gcaccgataa tcccggcgct ggccccttgc accataaaga
     7141 tcatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgctact tcgccctgct
     7201 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaacg gcgacaatca
     7261 ggctcagcag taaacccatc atattttttt ccatgcgtac ggcctggaac aactcgcctt
     7321 tacgatcacg ccagtcctgc catttgctgt cttcaggcag tttttgctga cttaatgagt
     7381 cgactttcag cggctcatcc agccacaaac gccagccggt aatattgcct gccggataac
     7441 gcatcagacg cgaggcatcc tcaatattca ccagcatttc attgccatcg acttcactgt
     7501 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
     7561 tgaactggct ggcagatggt accatcatgc ggatttgatc accgcgatta acgcctagct
     7621 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
     7681 tcacattgac cagatacggc gttagtggat ctttttgcgc cggatcgata cccagcatca
     7741 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
     7801 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
     7861 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
     7921 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
     7981 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt
     8041 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
     8101 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
     8161 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatgt tttatcatca
     8221 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
     8281 tcgggtcata acccgcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat
     8341 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc cggtggtgat
     8401 ttatcactcg gtcattacct tttatccgca tatgaccact tttccgcatc cgttatcgga
     8461 agtcctgagc aaatgctgga tctatttcaa tctttacctt gcaccctttc gtatgccggt
     8521 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
     8581 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
     8641 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
     8701 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
     8761 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
     8821 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
     8881 tcccacgccg tggtggggaa tgaacagtgt gatccgcaat ttgccttatt acagccttgg
     8941 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtaccgttgc gccgccatct
     9001 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
     9061 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
     9121 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
     9181 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
     9241 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
     9301 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
     9361 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactcccgct aagccatcgc
     9421 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
     9481 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
     9541 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
     9601 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
     9661 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagtc
     9721 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagct
     9781 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
     9841 tgcagcgtgg cgtactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
     9901 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
     9961 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    10021 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    10081 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    10141 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    10201 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    10261 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgccgggatc gagtactggc
    10321 agccattgtt cttcagcgaa ccactgccgc cgctgttcag ttatttccct gccaatacct
    10381 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    10441 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    10501 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa
    10561 ctaaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    10621 tagccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    10681 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    10741 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    10801 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc
    10861 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    10921 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    10981 agccggtggt ccatctggag cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag
    11041 cgggcggcat tactggcgag tatttgatgc tcacctatgc caacgacgcc aaactgtatg
    11101 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    11161 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    11221 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    11281 tcgcgtttaa acacgatcgt gagcagtatc agttgttctg cgacagcttc ccgtttgaaa
    11341 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    11401 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    11461 ccgctttcct ggcagtagat aaccacaagc aggtggcggt gttggtgcct accacccttc
    11521 tcgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    11581 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    11641 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    11701 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    11761 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    11821 gcacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    11881 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    11941 cgatcctgcg tgaaattttg cgcggggggc aggtttatta tctctacaat gatgtggaaa
    12001 acatccagaa agctgccgaa cggctggcag aactggtgcc agaagcgcgg atcgccatcg
    12061 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    12121 gtttcaacgt gctggtttgt acaaccatta tcgaaaccgg gatcgacatc ccgacagcca
    12181 acaccattat cattgaacgc gcggatcact tcggtctggc gcagctgcac cagttacgcg
    12241 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    12301 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcggtg
    12361 caggctttgc gctggcaacg cacgatctgg agatccgcgg cgcgggtgaa ctgcttggcg
    12421 aagaacaaag tggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    12481 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    12541 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatt cctgacgtga
    12601 atacgcgttt gtcgttctat aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    12661 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc
    12721 tggatgttgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    12781 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    12841 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    12901 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    12961 aactggaaga gaacgcgatc gcttaatggt aaatcggggg cgtttctgcg cccccatcgg
    13021 caacatttac aaattctttg cacttccctg cactatccga cactgtcacc atccataatt
    13081 caggcttatc tgtttattac aataacctta tatttattat ggatttttgg tgatgatcaa
    13141 aacgcgtttt tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct
    13201 accggcaaaa gccaacacct ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa
    13261 caaatttcat gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa
    13321 cgtcggcttt ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc
    13381 gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat
    13441 tgtgatcaac attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac
    13501 cgtgtatcca ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac
    13561 caccgtttca gacaaacgtg caaacccaac ctggacgcca acggcaaaca cccgcgcccg
    13621 ttataaagca cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat
    13681 gggccatcat gcgattcgtc tggcggccta tggcggcgtt tatttgcttc atggtacgaa
    13741 cgccgatttc ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga
    13801 tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc
    13861 gataaaagtc tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc
    13921 agagaagatt gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc
    13981 atttaaagat gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc
    14041 cgggatgccg gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga
    14101 taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta
    14161 atgagggtta atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt
    14221 acagaagtaa tacggaaaga ttttgcaccc atctcatccg ctttttgcgc cagctgctct
    14281 tccagcgatc ccagatttgt ccccgcttta gcactgattg taccgacttt ttgttggcct
    14341 tctggcgttg actgaacttc gacagccgca aagctggcaa atgacatgga gcttaaaatc
    14401 gccgcagcga tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg
    14461 tcgcaagcaa ttacttaacg atcgataact aaatgacaga tgtgatctgg atcacataca
    14521 agatattttt tataacaatc attaattaat ttaaaaatct ttaaatttca gttaaataga
    14581 aagcaattat ttttatggga tctggactgg tgaacggagg tgattatttt tataataatc
    14641 tttcattcac agtcaccagg tacaacggca tcatggcaac tgactcaaca caatgtgtaa
    14701 aaaaaagccg tggccgccca aaagtgttcg acagggatgc cgcgcttgat aaggccatga
    14761 aattgttctg gcaacacggt tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg
    14821 gagccaaagc gcccacgcta tacgcggaat ttaccaacaa agaggggtta tttcgtgccg
    14881 ttctcgaccg ctatatcgat cgttttgccg ctaagcatga agcacagctg ttttgtgaag
    14941 agaaaagcgt ggagtctgcg ctggctgact attttgctgc catcgccaac tgctttacca
    15001 gcaaagacac cccggcaggc tgcttcatga tcaacaactg caccaccctc tccccagatt
    15061 caggagatat cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc actttgcagc
    15121 agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc
    15181 tggcagaatt ccttaattgt attattcagg ggatgtcgat cagcgcacgc gaaggtgcat
    15241 cgctggaaaa actaatgcag attgccggaa cgactttgcg tttatggccc gaactggtga
    15301 aataagtcgc aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt gcagagtaca
    15361 gccttgttat actttgttat tcaaaatcag ttgcccatta ctatctagcg ggatctgcgt
    15421 acccggatcg cggtccatgc ggattttgcc ctgctgatcg ccaatcttat aggtcacatc
    15481 ataaccgagc attttttctg acttgtcata caccgtttta caacgctgtt gcgtagtcgt
    15541 gtaagtatcg ctttcctgga gagagccctg gatctggtta ccggcatatc caccacccag
    15601 cgcccccaca acagtggcga catctttacc gcgaccaccg ccaaactgat gccctatcac
    15661 gccgccagca acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg
    15721 acgatgggtc actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat
    15781 tggggttgca gaaacaacct gagcgtattg cgggccacgt tcaaacacgt tcagactggc
    15841 cactgccgct acgcccagcg cagctgcgac accaatcccg atacccgcca acattgattt
    15901 attcacggga acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc
    15961 agctatcaga cagaagatga aaagcgcggg aatggctgga aatacttaat gattcggagg
    16021 agtctgaagg aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc
    16081 atttgtccag ggcggatgag gcgtttatgc cacatccgcc agtgtacgtc gattaatgca
    16141 acttcaaacg cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt
    16201 taaagtaacc atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg
    16261 caattcgtcc ttcaatcatc attgagccgc gcgtcaagtt acccatcagg ctaccaacgg
    16321 tggagaagtt cgacagcgat accagtgaac catgatcttt atactgataa tttttcagcg
    16381 gcttaccgtt tatctgcgcc agaatattgt tcattgcgca agtcgccatc tggtgtgcag
    16441 cctgagcacg cggtggaaca aagccccctt ccggacgcgt gcatgacgcg cagtcgccaa
    16501 tagcgtaaat gtctggatcg tgggtggttt gcagcgtcgg ttccaccacc agctggttga
    16561 tacggttagt ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg
    16621 cccacaccat cagatcagcc tcaatatatt caccatcttt agtgtgcagg ccgccttcat
    16681 cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt aactcactat
    16741 gggccgcagc agagatgcgt ggcggtaatg caggcaaaat acgttctccc gcttctacta
    16801 gcgttacgtt cagggcttcg ttggtcagac ctttataacc gtagctgtgc agttgcttga
    16861 ccgcgttatg caactcagcg gagagttcta cacccgtcgc gccgccaccg acaatcgcaa
    16921 tgttcacttt gccattcgcg cccaggttgg cggagtattt caggaacaga ttcagcatct
    16981 cctggtggaa gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc
    17041 ctggcgtatt gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga
    17101 ttttacgttc cggaaccagc agttcacctt tctcgtcgcg tagttctgca atagtgattg
    17161 ttttcgcttc acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc
    17221 gcgcatgggc cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt
    17281 cgtgcagcag cggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt
    17341 ttttcttacg tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc
    17401 cgccgacaat cacaatcttt ttcaatggcg tagtcaacgt gacccccttg aatttattaa
    17461 ccaattgtta attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga
    17521 aaatgttctt cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt
    17581 taatcaagtt ttgttgctga aaagataggc gactttaccg ggtgagatca aaaaatagag
    17641 tttattgttt attatttgag cgaatttgtg agagtgatat agagtgaggg gtgtatgcag
    17701 ggcgttcggt aggcctgata agaggcgcat caggcattga tgctcgactg tcggatgcgg
    17761 cgtaaacgcc ttatccgact tacagaattc ggagcgcatc aggtaccggc aaggttacct
    17821 gatgcgacga gggcatttac ccgagggttt tgaacgcttt aatgcgctgc aaatgcgggg
    17881 agatattctt gaatttgtgc gtctgctctt cgtcccagac aatctcgtaa taatgatgca
    17941 actcttcaga tgtccgctgg ctgttaagcg cttcatcatt acgcgacaaa atcaccaggc
    18001 aacgatcgcg attcttctcg cggaagttgg tcacacactt agtggcaata tcggcatact
    18061 cttccgggcg atcaatcttg ccttccatgt tctcataagg gaacaaatta gggttgaaga
    18121 tcacctggcg gatatcgcaa agaaaaccaa tccgttccgc ccagtatccg cccagaccaa
    18181 cgccgcaaat cagcggacgc tcgtcgacgt tcagttgcaa cattttgtcc acttctttaa
    18241 gcagatgctg catatcatgt ttcggatgcc gcgtactgta gcttatcaag cgtacatccg
    18301 ggtcaataaa ctgcaattgt aagactttct cgtggttacc cggactgtta gagtcaaaac
    18361 cgtgtaaata gataatcatc gcttcctcac ataagccagg gttagtgacc tgctttctct
    18421 tcctgccagc gttcatgtaa ctgattcaga cgggtgctgc tcgccttcca gcgagccgag
    18481 tccatcagtt cctgtcgcga aaatgaacct ttatgataca aacgtgtaac acgttctgcc
    18541 ttgatcggtg acagattatc taacacgctg acggcccctt tacgattatt gcagaccagg
    18601 atcatatcgc aacctgcgtc cagtgatgcc tgcccgcgtt cggcataact gcccataatc
    18661 gcggcacctt ccatcgataa atcgtcagag aaaattacac cgtcaaaaca cagttcctga
    18721 cgcaaaacgg ttttcagcca gtaagaagaa ccgctcgccg gacgcggatc aacatcactg
    18781 tagatcacat gcgcaggcat aatggcgtcg agtttatttt cgcggattaa cgtgctgaaa
    18841 accgacatat ctttggcacg aatttccgct tgcgggcgtg ggtcgcacgg cgtctctttg
    18901 tgtgaatctg cagttactgc accgtgtcct gggaagtgtt tcccggtcgt tttcattccg
    18961 gcttcatgca tcccatcaat aaaccgacta gcgattgcca gggctttttc tgggtcggca
    19021 tgataagaac gctcgccaat cgccgcgctg atatgtccta catccagcac tggcgcaaag
    19081 ctgatatcaa tatccatagc gatcatttcg ctggccatca gccaacccgc ctcttgcgcc
    19141 agtttgccgc cctcttccat tcccaacagc gcagcaaagg attgtgctgc cggtaagcgg
    19201 gtaaaacctt cgcgaaaacg ctgcacgcgt ccaccttcct gatctaccgc caccaccaga
    19261 tgattgcgcg atgctgcgcg gatctggcgc accagttcac gtaactgggc aggatcatga
    19321 tagttacgcg taaagagaat cagccctccc accagcggat gcgccagtat ttcacgctct
    19381 tccgcgtcca gttcataacc ttcgacatcc aacattactg gacccacact gctctcctta
    19441 ttgttttatt aatagctgcc gccaggtgtc atcggccagc ctgataaatt gttgatcgcc
    19501 ggtttgtcgc cagcggtact caaaccaccc tgctttgagc atcagcagcc agggaaacca
    19561 tcgcctgacc tgacgccata attgcgccgg ataaatcttc gcgcgagtgg catagtcatt
    19621 gaccaattgc cggtgctgtt cagtattttc cacccacacc gccgccagtt ccagcgcggt
    19681 atcaccatct ccggcatact cccagtcgat gagttttaac cctgatgcgc tatgcactaa
    19741 atttccggcg tggacatcca tatgcaatgg acttaagcgt aaaggtcgtg gttcccgcgc
    19801 tttgcgcaga cgttttaaca ttcgcagcca acccacagtc cgccgcgccg gatcgctttg
    19861 ctgccagtac agttccagta acggcaacag cgttattcgc cagccaaaac gtggttgttg
    19921 atgtagataa tacagcaagc ctgccagttc gttggtatcc ggcaaatacg tttttaccgc
    19981 gccgggcaga tagtcgacta ccatccagtc acggagatat aaatgcggct tcggtgcaat
    20041 gcttgcgggt agttgtgata aagcccgata ctggcgcaag aacgcggact gcggcgcatc
    20101 aggatcgtgc ggctgacgca caacaaaacg ctggccctga tgttcaatga gaaaactacc
    20161 gccactaagc ccactattaa acgtcgtgac gggatgaaac tgcgggaaaa agcgcgacag
    20221 caattcgtcg cgcgtgatgg gattattact gcgaaacggc acctttacct gaccagataa
    20281 tttcgcccgt ctgcaccagc atcagctgca tttgtagggt cggagcgtta acgttgccag
    20341 aggcgctgga gtacagcacg taatgagcgc cgacattgcg ggcaatgcct atggctttac
    20401 tacgggtgcc taaactgtcc tgcggcgaca aacctaactg ttgcttagcc atcgacagtt
    20461 gctgggcgga aaccagggta aatttcccgt tattagccag cgcatttcgc agcgtttcgg
    20521 tcgcttctgc ggcattcagc gaaccgttag tacggttgtt aacgctatca accagcagga
    20581 cgctacccgc agtcaccccg tcagccccaa gcatcttact gactatcggc tgcattgcgc
    20641 cattccagtc ataatggcga atatgcggcg caggcggtgc cgttcgatct tcgtgctcaa
    20701 ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac tgtggggaca ggctgttgtg
    20761 gctcggctgg ttgttccggc gctggtttca cttcttctac cggtgcaggt tcacgttgcc
    20821 ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa ggcgtagcga ctcatttttg
    20881 tcatcaagat tcacccctta caaatataga taaagtctga ctttgtgcgc ccccagaaaa
    20941 ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa tggtaacgct gcgtggcctt
    21001 tccagaggat gcatctccag ccctctggcg tcataccagt aaaaacgata atgaacggta
    21061 acgggttttt gcctttcgtt ataaagcgtt gaggatgctg aaggttgaat atcagacgtc
    21121 gaaaggacgg gcttttctgc actgatgccc gcagccaata aagatgactc catcaccagc
    21181 gattgctcat cattcaccgg aatttccgga tgtgaacgac agcccaccag cagtaacaac
    21241 accagagaca ccagcccaaa gcatcctttt ctcatcgtta cagaccttta tgcgccagca
    21301 ttggtcccag cggacggcca cccaacaagt gcatatggat gtggtaaacc tcttgtccgc
    21361 catggcggtt ggtattcatg atcagacgat agccatcttc ggcaatacct tcttgctcag
    21421 caatttttgc cactacggtg atcatgcgtc ccagcgcctg ctcatgctca gctgagacgt
    21481 cgttcacagt cggaatgagg atattcggaa tgatcagaat atgcgttggc gcttgcggcg
    21541 aaatatcgcg aaacgccgtt accagatcat cctggtagac gatatcggag gggatctcac
    21601 gacgaataat tttgctgaat atagtttctt ctgccacgac attttccttt ttcaaatagc
    21661 catgcgttgc gccatgctac gccgcgccac actgcgagta tagagtatgg tcgagttaaa
    21721 cctgctcttt caaccttaac tcacgatttc ttaagcaaaa aatcgttttg ctgccgtata
    21781 tatcgccatt attcccattt ctgcctgcga atgcgctgaa aagacattct ctgtttggtt
    21841 tacacgacaa atatgaatgc gtatatttct catttgcatt tacaaacaaa attattcgca
    21901 cactaaatat aatcgctcat caccctcctc cggcatgagc ctgacgacaa cataaaccat
    21961 gagatttcaa atgctttcaa cacaatttaa cagggataat caatatcaag ccatcacc
//
LOCUS       DAJTYD010000054        21288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTYD010000054 DAJTYD010000000
VERSION     DAJTYD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.054"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(249..1226)
                     /gene="epmA"
                     /locus_tag="OO585_001847"
     CDS             complement(249..1226)
                     /gene="epmA"
                     /locus_tag="OO585_001847"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6629459.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1551..3359
                     /gene="frdA"
                     /locus_tag="OO585_001848"
     CDS             1551..3359
                     /gene="frdA"
                     /locus_tag="OO585_001848"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6629460.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3352..4086
                     /gene="frdB"
                     /locus_tag="OO585_001849"
     CDS             3352..4086
                     /gene="frdB"
                     /locus_tag="OO585_001849"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6629461.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4097..4492
                     /gene="frdC"
                     /locus_tag="OO585_001850"
     CDS             4097..4492
                     /gene="frdC"
                     /locus_tag="OO585_001850"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6629462.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4503..4862
                     /gene="frdD"
                     /locus_tag="OO585_001851"
     CDS             4503..4862
                     /gene="frdD"
                     /locus_tag="OO585_001851"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6629463.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4925..6058
                     /locus_tag="OO585_001852"
     CDS             4925..6058
                     /locus_tag="OO585_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6629464.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6147..6680
                     /gene="blc"
                     /locus_tag="OO585_001853"
     CDS             6147..6680
                     /gene="blc"
                     /locus_tag="OO585_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6629465.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6677..6994)
                     /gene="sugE"
                     /locus_tag="OO585_001854"
     CDS             complement(6677..6994)
                     /gene="sugE"
                     /locus_tag="OO585_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6629466.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7176..7322)
                     /gene="ecnB"
                     /locus_tag="OO585_001855"
     CDS             complement(7176..7322)
                     /gene="ecnB"
                     /locus_tag="OO585_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6629467.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7433..7558)
                     /gene="ecnA"
                     /locus_tag="OO585_001856"
     CDS             complement(7433..7558)
                     /gene="ecnA"
                     /locus_tag="OO585_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6629468.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7610..8176)
                     /gene="efp"
                     /locus_tag="OO585_001857"
     CDS             complement(7610..8176)
                     /gene="efp"
                     /locus_tag="OO585_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6629469.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8218..9267
                     /gene="epmB"
                     /locus_tag="OO585_001858"
     CDS             8218..9267
                     /gene="epmB"
                     /locus_tag="OO585_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6629470.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            9641..10510
                     /locus_tag="OO585_001859"
     CDS             9641..10510
                     /locus_tag="OO585_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6629471.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10713..11066)
                     /locus_tag="OO585_001860"
     CDS             complement(10713..11066)
                     /locus_tag="OO585_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6629472.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11204..12850)
                     /gene="groL"
                     /locus_tag="OO585_001861"
     CDS             complement(11204..12850)
                     /gene="groL"
                     /locus_tag="OO585_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6629473.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(12894..13187)
                     /locus_tag="OO585_001862"
     CDS             complement(12894..13187)
                     /locus_tag="OO585_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6629474.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13463..14719
                     /gene="yjeH"
                     /locus_tag="OO585_001863"
     CDS             13463..14719
                     /gene="yjeH"
                     /locus_tag="OO585_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6629475.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(14735..15211)
                     /gene="fxsA"
                     /locus_tag="OO585_001864"
     CDS             complement(14735..15211)
                     /gene="fxsA"
                     /locus_tag="OO585_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6629476.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            15548..16984
                     /gene="aspA"
                     /locus_tag="OO585_001865"
     CDS             15548..16984
                     /gene="aspA"
                     /locus_tag="OO585_001865"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6629477.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17102..18403
                     /gene="dcuA"
                     /locus_tag="OO585_001866"
     CDS             17102..18403
                     /gene="dcuA"
                     /locus_tag="OO585_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6629478.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18519..18857
                     /gene="cutA"
                     /locus_tag="OO585_001867"
     CDS             18519..18857
                     /gene="cutA"
                     /locus_tag="OO585_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6629479.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18833..20530
                     /gene="dsbD"
                     /locus_tag="OO585_001868"
     CDS             18833..20530
                     /gene="dsbD"
                     /locus_tag="OO585_001868"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6629480.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20567..21142
                     /locus_tag="OO585_001869"
     CDS             20567..21142
                     /locus_tag="OO585_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6629481.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         5230 a         5493 c         5336 g         5229 t
ORIGIN      
        1 cagactcatc ttttttatcg tgtgaggcat gaaaccatcc ttcatttcag cgcgtgtata
       61 acacgtgccc actgaactgc actgcgcaac attgagcagg cagcgtaaaa accaatacag
      121 aattgatacc cggatgatat caaatatcca gtcttaaatt gtggccttta acggaaaaat
      181 gaaaacatat gctacagaat ggcgcttatc acgccattct tcgctgttaa ttcagtaatt
      241 tttcagaatt atgcccggtc aacgctaaag gcgatgactt cagccagtgt ctccgcgccc
      301 agcgccaaca tcaccagacg atcaacacct aatgccacgc cggaacagtc aggcataccg
      361 actttcaagg cttcaatcag attctggtca atggggtgct gcggcaaacc gcgcgccgcg
      421 cgcttacggt tatcttgttc aaagcgttgt tgctgctcac gggcatccgt taattcatgg
      481 aaaccattcg ccagctcaat acctttataa taaacctcaa agcgttcagc gacccgatga
      541 tcttcggtac tgatttgcgc cagtgatgcc tggctggctg gaaagtggta cacaaaggtc
      601 ggtttttctt tgccaatatt tggctctacg ccaaaggtaa acagcaattg tagcaacgtg
      661 tcgcggtctt cttcggtatc agcaacgtta ctcaaatcca gtttcgctgc gacttcccgc
      721 agttgcgttt tgtcggcaga gagcgggtca atttccagat aacgcaagaa agcttgttga
      781 taagaaaggc tttctgctgc cgggcagtcc agcacctgtt gtaagagatc gtccacctcg
      841 ttcatcaacc ggtacatatc atagtgcggt cgataccact caagcatagt gaactcaggg
      901 ttgtgataac gccccatctc ttcattacgg aagctgcggc acagctggaa taccggccca
      961 caaccggcaa ccagcaggcg tttcatatgg tattccgggc tggtcattaa ccagagattc
     1021 atcccctgcg aatgcccggg gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg
     1081 gttaccgtcg cctggctcat acaaggcgtc tccacctcca gcactccacg atcagcaaag
     1141 aaacgacgga tctccgccat aatcgccgcg cgttttaata agttaggaat ggatgcgctc
     1201 ggctgccagg atgccgtttc gctcatagtt aaatctccag tttttgacaa gggcacgaag
     1261 tctactcgca acgcgacggc gagacaaatt ttacgcagga atcaaacagc ggtgggcagt
     1321 gactaaaaaa agcacgatct gatggtttag taattaaatt aatcatcttc agtgataatt
     1381 tagccctctt gcgcactaaa aaaatcgatc tcgtcaaatt tcagacttat ccatcagact
     1441 atactgttgt acctataaag gagcagtgga atagcgttcg cagaccgtaa ctttcaggca
     1501 cttaccctga agtacggggc tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct
     1561 ttcaagccga tcttgccatt gtaggcgccg gtggcgcggg attacgtgct gcaattgctg
     1621 ccgcgcaggc aaatccaaat gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta
     1681 gccataccgt tgctgcagaa gggggctccg ccgctgtcgc gcaggatcat gacagcttcg
     1741 aatatcactt tcacgataca gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg
     1801 attatttcgt ccaccactgc ccaaccgaaa tgacccaact ggaactgtgg gggtgcccat
     1861 ggagccgtcg cccggatggt agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc
     1921 gcacctggtt cgccgccgat aagaccggct tccatatgct gcacacgctg ttccagacct
     1981 ctctgcaatt cccgcagatc cagcgttttg acgaacattt cgtgctggat attctggttg
     2041 atgatggtca tgttcgcggc ctggtagcaa tgaacatgat ggaaggcacg ctggtgcaga
     2101 tccgtgctaa cgcggtcgtt atggctactg gcggtgcggg tcgcgtttat cgttacaaca
     2161 ccaacggcgg catcgttacc ggtgacggta tgggtatggc gctaagccac ggcgttccgc
     2221 tgcgtgacat ggaattcgtt cagtatcacc caaccggtct gccaggttcc ggtatcctga
     2281 tgaccgaagg ctgccgtggt gaaggcggta ttctggtcaa caaaaatggc taccgttatc
     2341 tgcaagatta cggcatgggc ccggaaactc cgctgggcga gccgaaaaac aaatatatgg
     2401 aactgggtcc acgcgacaaa gtttctcagg ccttctggca cgaatggcgt aaaggcaaca
     2461 ccatctccac gccgcgtggc gatgtggttt atctcgactt gcgtcacctc ggcgagaaaa
     2521 aactgcatga acgtctgccg ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc
     2581 cggttaaaga accgattccg gtacgtccga ccgcacacta caccatgggc ggtatcgaaa
     2641 ccgatcagaa ctgtgaaacc cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg
     2701 ttggtctgca cggtgcaaac cgtctgggct ccaactccct ggcggaactg gtggtcttcg
     2761 gccgtatggc cggtgaacaa gcgacagagc gtgcagcaac tgccggtaat ggcaacgaag
     2821 aggcaattga agcgcaggca gctggcgttg aacaacgtct gaaagatctg gttaaccagg
     2881 atggcggcga aaactgggcg aagatccgcg acgaaatggg cctagcaatg gaagaaggtt
     2941 gcggtatcta ccgtacgccg gaactgatgc agaaaaccat cgacaagctg gcagagctgc
     3001 aggaacgctt caagcgcgtg cgcatcaccg acacctccag cgtgttcaac accgacctgc
     3061 tctacaccat tgaactgggc cacggtctga acgttgctga atgtatggcg cactccgcaa
     3121 tggcacgtaa agagtcccgc ggcgcacacc agcgtctgga cgaaggttgc accgagcgtg
     3181 acgacgtcaa cttcctcaaa cacaccctcg ccttccgcga tgctgatggc acgactcgcc
     3241 tggagtacag cgacgtgaag attactacgc tgccgccagc taaacgcgtt tacggtggcg
     3301 aagcggatgc agccgataag gcggaagcag ccaataagaa ggagaaggcg aatggctgag
     3361 atgaaaaacc tgaaaattga ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat
     3421 agcgcattct atgaagtgcc ttatgacgca actacctcat tactggatgc gctgggctac
     3481 atcaaagaca acctggcacc ggacctgagt taccgctggt cctgccgtat ggcgatttgt
     3541 ggttcctgcg gcatgatggt taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt
     3601 gattacaccg acggtatgaa ggttgaagcg ttagctaact tcccgattga acgcgatctg
     3661 gtggtcgata tgacccactt catcgaaagt ctggaagcga tcaaaccgta catcatcggc
     3721 aactcccgca ccgcggatca gggtactaac atccagaccc cggcgcagat ggcaaagtat
     3781 caccagttct ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc
     3841 ctgaacccag agttcatcgg tccggctgcc attacgctgg cgcatcgtta taacgaagat
     3901 agccgcgacc acggtaagaa ggagcgtatg gcgcagttga acagccagaa cggcgtatgg
     3961 agctgtactt tcgtgggcta ttgctccgaa gtctgcccga aacacgtcga tccggctgcg
     4021 gccattcagc agggcaaagt agaaagttcg aaagactttc ttatcgcgac cctgaaacca
     4081 cgctaaggag tgcaacatga cgactaaacg taaaccgtat gtacggccaa tgacgtccac
     4141 ctggtggaaa aaattgccgt tttatcgctt ttacatgctg cgcgaaggca cggcggttcc
     4201 ggctgtgtgg ttcagcattg aactgatttt cgggctgttt gccctgaaaa atggcccgga
     4261 cgcctgggcg ggattcgtcg actttttaca aaacccggtt atcgtgatca ttaacctgat
     4321 cactctggcg gcagctctgc tgcacaccaa aacctggttt gaactggcac cgaaagcggc
     4381 caatatcatt gtaaaagacg aaaaaatggg accagagcca attatcaaaa gtctctgggc
     4441 ggtaactgtg gttgccacca tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg
     4501 agatgattaa tccaaatcca aagcgttctg acgaaccggt attctggggc ctcttcgggg
     4561 ccggtggtat gtggagcgca attattgcgc cggtgatgat cctgctggtg ggtattctgc
     4621 tgccactggg gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg gcgttcgcgc
     4681 agagcttcat tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt
     4741 tacaccgtat gcaccacgcg atgcacgatc tgaaaattca cgtgcctgcg ggcaaatggg
     4801 ttttctacgg tctggctgct atcctgacag ttgtcacgct gattggtatc gttacaatct
     4861 aacgtatcgc caatgtaaat ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga
     4921 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
     4981 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
     5041 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
     5101 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
     5161 ttcggtcagc aaaacattta cgggcgtgct tggtggcgat gctattgctc gaggggaaat
     5221 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
     5281 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc agatgccgga
     5341 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
     5401 tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
     5461 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
     5521 actcaaccat acgtggatta atgtaccgcc cgcagaagaa aagaattacg cctggggata
     5581 tcgcgaaggc aaggcagttc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
     5641 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc cccttgatat
     5701 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
     5761 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
     5821 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
     5881 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     5941 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     6001 ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     6061 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     6121 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     6181 ctgcatttct ggtcgttgcc tgcagttctc ctacgccgcc gcgtggcgtg acagtggtga
     6241 ataatttcga cgctaaacgc tatctcggta cctggtatga gattgcccgt tttgatcacc
     6301 gttttgaacg tggactggaa aaagtcaccg caacatacag tctgcgtgat ggcggcggcc
     6361 tgaatgtcat caataaaggc tataaccctg acagagaaat gtggcagcag agtgaaggga
     6421 aagcgtactt taccggcgca ccaacacgcg ctgcgctgaa agtgtcattc tttggtcctt
     6481 tctatggcgg ttataacgtc attgcacttg atcgggaata ccgccatgcg ctggtttgcg
     6541 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     6601 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     6661 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     6721 acgccagact cgccaggcgc atcgggttag cggactcacc gagcagcaca atgccggtga
     6781 tagccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct actggtaacg
     6841 atttcatcgc ccaggcaagt agcgccatac tgacaatcat cgccgtcacg gtaataacac
     6901 tcggcgtcaa acgactaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     6961 gcagaccagc aataactaag ataatccagg acatatcagg ctccggagaa ttggggccgt
     7021 ccccggtgaa aagacgcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     7081 tgccaggtgg caggggaatt tcaactatta ccttagtgac tatcgcagaa aaaacaaacg
     7141 gcacgacaca gctgtcgtac cgttattgct tattgctatt gctgcgcttt cgttgctgcg
     7201 ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag
     7261 gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt cttcttcacc
     7321 ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt aataatagac
     7381 aacatcgcca aaaatgacgg aaatctgatg atttttcgga agagaagaaa aattagctgg
     7441 cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt
     7501 tacagcccgt gagcagcgta ctggcaagca aaacaagaac gataaggcgt ttcatcatct
     7561 ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat tacttcacgc
     7621 gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca
     7681 gcggaacctt aaccacagcg ccagtagaca gggtagccgg tttgccgcca gtacctgcgg
     7741 tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag ttcggcggag
     7801 taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga tccagcagcc
     7861 atttagcgtt atcgccaatt gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt
     7921 tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata tcgacaacat
     7981 cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta ccggtcagca
     8041 gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg aattcactcg
     8101 cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga aaatcgttgc
     8161 tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg
     8221 gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca acttgccgat
     8281 gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga cgaaaaactg
     8341 ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc atttatcgat
     8401 cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac ctcgcaagat
     8461 gagtttgtcg tcgcgtccgg attctccacc gacccactgg aagaacagca cagcgtagtg
     8521 cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg
     8581 gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag
     8641 cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga cgagatgatt
     8701 ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt gctcacacaa
     8761 ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc gattgtgatc
     8821 ccggcacgca tcacagatgc gctggttgaa cgcttttcac attctacgct gcaaatcctg
     8881 ctggtgaatc acatcaacca tgccaatgag atagatgaaa cattccgtca ggcgatggct
     8941 aagttgcgcc gggtaggcgt tactttgctg aaccagagcg ttctgttacg tgatgtgaac
     9001 gataacgcac aaacgctggc aaacctgagt aatgcgttgt tcgatgccgg cgtgatgccg
     9061 tattacctgc atgtgctcga taaagtacag ggcgcagcgc attttatggt gagtgatgac
     9121 gaagcacggc agattatgcg tgagttgctg acattggtgt cgggatatct ggtgccgaaa
     9181 ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca gctacgccag
     9241 cagttactga ttaaacaggg aagttaaaca ttaaaattgc ctgatgcgct acgcttatca
     9301 ggcctacatc gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg
     9361 tttacgccgc atccggcaag agcaacaaac gcaagtacag caacctgtca ttaaactgag
     9421 aaatttaatc tttgcccgtt gcataacctt taattattca tatgcattgc tgttgttact
     9481 ttttatatag ctaacaccaa taattaacct ttcttttctg tttatataga gtgagcacgg
     9541 ctatttatgt tattttaaca actattacta ccaagcccat ttatctggct tataaattaa
     9601 aattaattat ttaattgaga aatggttagg gagaacctac atggccataa gcatcaaggg
     9661 tgttaatacc ggtgttattc gcaagagtaa taatttcatt gcactggcgc tgaaaattaa
     9721 agaacctcgc aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct
     9781 gattgcgctg gaaagtcgtc tgcatcaaaa gcataaactg gatgctgctg cacgccttca
     9841 gtatgagcaa gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct
     9901 ggttgatgag cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga
     9961 taatcaaggc gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt
    10021 agtcgtcaat gagttgcaga ttgaaatgct agcgcgagca atcattcatg caattaataa
    10081 tgcggaaatg cgtgaactgg cattacgtat tacctcactg ttagatttcc tgccgttata
    10141 tgacgttgac tgccaggaaa atggcaatct ggaatatgac acctattccc aaccggaatg
    10201 gaaacataac ttgttcgacc attatctggc ggtgctttac cgctttaaag atgaaagtgg
    10261 caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaagt
    10321 agaggcgatc actcgccgaa tgcttgattt cagcccacga ctgaaaaaac tcgcgggtgt
    10381 gccatgtcag gtctatgttc gcaccgtagc ggcgaataac gcccaaccgc taacccagga
    10441 tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca
    10501 ggcgaaataa tctcatcttt gctaatgcga ggctggagcc atccagcctg aaatgactga
    10561 taacgtgatc gttggtcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct
    10621 caagcattca atcagttgca atgcctgctg taggcctgat aagcgaagcg catcaggcaa
    10681 taatacgttt gtcacccagc ctctggatca catcagttcg ggcacttata aacctgaccg
    10741 ataatctggc tatccgtcgg gacaaaactg gacaacattc cctgcgacgg gctgctgatg
    10801 ccataaatca cgttaccgcc cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg
    10861 cgcatagaac cgccctcttc tccgtgttgc ccggaaagcc agttgctttg cttacctgtc
    10921 gcagtaccaa tcagctggca ctctgcgcct ggctgctcgt ccacaatgcg tacactctga
    10981 ccggcagcac tcaattcgtt gctggagcta caacccgcca tcagtagcgc ggcaccgaca
    11041 atccctgcta agtattttac gtgcatgtta ttccccataa tcaatgagct ggacgccact
    11101 cgcgtcgtcc gtgtctgaat cttatactag aaagatgacc aaaagaaaaa cccccagaca
    11161 tttctgcccg ggggtttgtt tatttctgcg aggtgcaggg caattacatc atgccgccca
    11221 tgccacccat gccgcccata ccgccagcag cgcctaagtc agctgcatcg tttttcggca
    11281 ggtcggtaac catgcactcg gtggtgatca tcaggccagc cacagaagcc gcgtactgca
    11341 gagcagaacg ggttactttg gttggatcca agatacccat gtcgatcatg ttgccgtatt
    11401 cttcggttgc tgcgttgtaa ccgtagttgc cgtcgccgcc tttaacagtg ttagcaacaa
    11461 cagacggttc ttcgccgcag ttcaatacga tctgacgcag cggagcttcc attgcacgca
    11521 gtgcaacttt gatacccacg ttctggtctt cgttctgacc acgcaggtca gccagtttag
    11581 acgctacgcg gatcagcgca acaccaccac cagcaaccac gccttcttct accgcagcac
    11641 gggtcgcgtg cagggcatct tcaacgcgtg cttttttctc tttcatttca acttcggtag
    11701 cagcacctac tttgataact gcaacgccgc ctgccagttt cgctacgcgc tcctgcagtt
    11761 tttcacggtc gtagtcagaa gttgcttctt caatctgctg acggatctga gcaacacggc
    11821 cctggattgc agcttcttca cccacgccat caatgatggt ggtggtgtct ttgttgatca
    11881 caacacgttt agcctgaccc aggtcttcca gggttgcttt ttccagctcc ataccgatct
    11941 cttcagagat tacggtaccg ccagtcaggg ttgcgatatc ctgcagcata gctttacggc
    12001 gatcgccgaa gcccggagct ttaaccgcag caactttcac gatgccacgc atggtgttaa
    12061 caaccagagt tgccagcgct tcgccttcaa catcttcagc gatgatcagc agcggtttac
    12121 ctgctttagc aacggcttcc agaaccggca gcatttcgcg gatgttggag attttcttgt
    12181 cagccagcag gatgaacggg ctttccagtt ctactgcgcc agtttccggc ttgttgatga
    12241 agtaaggaga caggtagcca cggtcgaact gcataccttc aaccacgtcc agttcgtcct
    12301 gcagaccggt accgtcttca acggtgataa cgccttcttt accgactttg tccatcgctt
    12361 cagcgatcag tttacctacg gtttcgtcgg agttagcgga gatggtacca acctgagcaa
    12421 tcgctttaga gtcagagcat ggtacggaca gcgctttcag ttcttcaact gcagcggtaa
    12481 cagctttgtc gataccacgt ttcaggtcca tcgggttcat gcccgcagca actgctttca
    12541 gaccctcagt gatgatagcc tgagccagta cggttgcagt ggtggtaccg tcgcctgcag
    12601 cgtcgttcgc tttagaggca acttctttca ccatctgcgc acccatattt tcgaacttgt
    12661 cttccagttc gatttcacga gcaacggaaa caccatcttt ggtgatggtc ggtgcaccga
    12721 aagatttatc cagaactacg ttacggcctt tcggaccgag ggtaactttc actgcatctg
    12781 ccagtacgtt tacgccgcgc agcattttca cacgagcgtc gttaccgaat tttacgtctt
    12841 tagctgccat tatctttatt ccttaaattc gtatgttcag tgtcgtgcga ggattacgct
    12901 tcaacaattg ccagaatgtc gctttcggac atgatcaaca cttcttcatt gtcgatcttc
    12961 tcagatttca caccgtagcc atcgttgaaa ataacgatgt cgccaacttt cacatccagc
    13021 ggcttcactt cgccattttc aaggatacgg ccattgccga cagccagcac ttcgccgcgg
    13081 gtggatttag ccgctgcaga gccggtcaga acgatgccgc cagcagattt agtttcaact
    13141 tctttacgct tgacgatcac gcgatcatgc aatggacgaa tattcattga taactctcct
    13201 ttgagaaagt ccgtatctgt tatgggtgac gccggagctt acgtggtttc ccggctggtg
    13261 accagagaaa tggggatgag gattcgcccc ttcaaggggg aaaaagaaaa aaaattctga
    13321 tcagcacaaa atcgggtgaa aaccctgatt cacctcacat ttcatcgcaa tttcttcatc
    13381 gccgtcataa gcgaatctga ttgtgctacc atcgaaaatc tacgcagttg cccaaaattt
    13441 gggcgcaatc ggatatcaac ccatgagtgg actcaaacaa gaactggggc tggcccaggg
    13501 cattggcctg ctatcgacgt cattattagg caccggcgtg tttgccgttc ctgcgttagc
    13561 tgcacaggta gcaggcaata acagcctgtg ggcgtggccc gttttgatta tcttagtgtt
    13621 cccgattgcg attgtgtttg cgattctggg tcgccactat ccctgcgcag gcggtgtcgc
    13681 gcacttcgtc ggtatggcgt ttggttcgcg gcttgagcga gtcaccggct ggttgttttt
    13741 atcggtcatt cccgtgggtt tgccagccgc gctacaaatt gccgccgggt tcggccaggc
    13801 aatgtttggc tggcatagct ggcaactgtt gttggcagaa ctcggtacgc tggcgctggt
    13861 gtggtatatc ggtactcgca gtgccagttc cagtgctaat ctacaaaccg ttattgccgg
    13921 acttatcgtc gcgctgattg tcgctatctg gtgggcgggc gatatcaaac ctgcgaatat
    13981 ccccttcccg gcacctggta atatcgaact taccgggtta tttgctgcgt tatcagtgat
    14041 gttctggtgt tttgtcggtc tggaagcatt tgcccatctt gcctcggaat ttaaaaatcc
    14101 agagcgtgat tttcctcgcg cattgatgat tggtctgctg ctggcaggat tagtctactg
    14161 gggctgtacg gtagtcgtct tacacttcga cgcctatggt gaaaaaatgg cggcggcagc
    14221 atcgcttcca aaaattgtag tgcaactgtt cggtgtagga gcgttatgga ttgcctgcgt
    14281 gattggctat ctggcctgct ttgccagtct caacatttat atacagagct tcgcccgcct
    14341 ggtctggtcg caggcgcaac ataatcctga ccactacctg gcacgcctct cttctcgcca
    14401 tatcccgaat aatgccctca atgcggtgct cggctgctgt gtggtgagca ctttggtgat
    14461 tcatgcttta gagatcaatc tggacgctct tattatttat gccaatggca tctttattat
    14521 gatttatctg ttatgcatgc tggcaggctg taaattattg caaggacgtt atcgactact
    14581 ggcggtggtt ggcgggctgt tatgcgttct gttactggca atggtcggct ggaaaagtct
    14641 ctatgcgctg atcgtgctgg cggggttatg gctgttgctg ccaaaacgaa aaacgccgga
    14701 aaatggcata atcacataat ccggcgtttc gacattaatc ctggcgatcg tctttatgat
    14761 caaggcggtc gcgctcatca tcctttcgct ggtactcacc atcaaaagta ttaccgccac
    14821 cggtcccggc gctaaaaccg ccgcctggca tacgagaaaa gcgcaaatgc ggcatcaact
    14881 tcactgtcag atgcttttgc accggcggta ataaaagcag aagaccgagg aagtcggtga
    14941 aaaagcccgg taataaaagc agcaaaccgg caatgatcag cgaaacactt ttaatcatct
    15001 ccgccgctgg gttttcaccc gccgccattt tttgctgcat cagcacgaaa ttcttaaagc
    15061 cctggttacg taccagtgac atcccgataa ctgacgtgaa tataaccagc acgagggtca
    15121 gcaatacccc caatacatgg gcaacctgaa taaagattga aatctcaata tagacataaa
    15181 ggaaaatggc aataaaaggt aaccagcgca aaggtttctc ctgtaatagc agccggtaaa
    15241 acccggctac ctgaatgggt tgcgaatcgc gttcagctta tattgtggtc attagcaaaa
    15301 tttcaagatg tttgcgcaac tatttttggt agtaatccca aagcggtgat ctatttcaca
    15361 aattaataat taaggggtaa aaaccgacac ttaaagtgat ccagattacg gtagaaatcc
    15421 tcaagcagca tatgatctcg ggtattcggt cgatgcaggg gataatcgtc ggtcgaaaaa
    15481 cattcgaaac cacatatatt ctgtgtgttt aaagcaaatc attggcagct tgaaaaagaa
    15541 ggttcacatg tcaaacaaca ttcgtatcga agaagatctg ttgggtacca gggaagttcc
    15601 agctgatgcc tactatggtg ttcacactct gagagcgatt gaaaacttct atatcagcaa
    15661 caacaaaatc agtgatattc ctgaatttgt tcgcggtatg gtaatggtta aaaaagccgc
    15721 agctatggca aacaaagagc tgcaaaccat tcctaaaagt gtagcgaatg ccatcattgc
    15781 cgcatgtgat gaagtcctga acaacggaaa atgcatggat cagttcccgg tagacgtcta
    15841 ccagggcggc gcaggtactt ccgtaaacat gaacaccaac gaagtgctgg ccaatatcgg
    15901 tctggaactg atgggtcacc agaaaggtga atatcagtac ctgaacccga acgaccatgt
    15961 taacaaatgt cagtccacta acgacgccta cccgaccggt ttccgtatcg cagtttactc
    16021 ttccctgatt aagctggtag atgcgattaa ccaactgcgt gaaggctttg aacgtaaagc
    16081 tgtcgaattc caggacatcc tgaaaatggg tcgtacccag ctgcaggacg cagtaccgat
    16141 gaccctcggt caggaattcc gcgctttcag catcctgctg aaagaagaag tgaaaaatat
    16201 ccaacgtacc gctgaactgc tgctggaagt taaccttggc gcaacagcaa tcggtactgg
    16261 tctgaacacg ccgaaagagt actctccgct ggcagtgaaa aaactggctg aagtcactgg
    16321 cttcccatgc gtaccggctg aagacctgat cgaagcgacc tctgactgcg gcgcttatgt
    16381 tatggttcac ggcgcgctga aacgcctggc tgtgaagatg tccaaaatct gtaacgacct
    16441 gcgcttgctc tcttctggcc cacgtgccgg cctgaacgag atcaacctgc cggaactgca
    16501 ggcgggctct tccatcatgc cagctaaagt aaacccggtt gttccggaag tggttaacca
    16561 ggtatgcttc aaagtcatcg gtaacgacac cactgttacc atggcagcag aagcaggtca
    16621 gctgcagttg aacgttatgg agccggtcat tggccaggcc atgttcgaat ccgttcacat
    16681 tctgaccaac gcttgctaca acctgctgga aaaatgcatt aacggcatca ctgctaacaa
    16741 agaagtgtgc gaaggttacg tttacaactc tatcggtatc gttacttacc tgaacccgtt
    16801 catcggtcac cacaacggtg acatcgtggg taaaatctgt gccgaaaccg gtaagagtgt
    16861 acgtgaagtc gttctggaac gcggtctgtt gactgaagcg gaacttgacg atattttctc
    16921 cgtacagaat ctgatgcacc cggcttacaa agcaaaacgc tatactgatg aaagcgaaca
    16981 gtaatcgtac agggtagtac aaataaagaa ggcacgtcag atgacgtgcc ttttttcttg
    17041 tgaacagtaa cttaaaaaca acaatctaat atcaacttgt taaaaaacaa ggaaggctaa
    17101 tatgctagtt gtagaactca tcatagtttt gctggcgatc ttcttgggcg ccagattggg
    17161 gggaataggt attggttttg caggcggatt gggggtgctg gttcttgccg ctattggcgt
    17221 taaacccggt aacatcccgt tcgatgtcat ttccattatc atggcggtta tcgccgctat
    17281 ttctgccatg caggttgctg gcggtctgga ctatctggtt catcagacag aaaagctgct
    17341 gcgccgtaac ccgaaataca tcacgatcct cgcacctatc gtgacctatt tcctgacaat
    17401 ctttgctggt actggcaaca tctctctggc gacactgcca gttatcgctg aagttgcgaa
    17461 ggaacaaggc gttaaacctt gccgtccgct ctctactgca gtggtatccg cgcagattgc
    17521 gatcaccgca tcgccaatct cagcggcagt ggtttacatg tcttccgtga tggaaggtca
    17581 tggcatcagc tacctccatc tgctctccgt ggtcatcccg tccaccctgc tggcggttct
    17641 ggtgatgtcc tttctggtca ctatgctgtt caactccaaa ctctctgacg atccgattta
    17701 tcgcaagcgt ctggaagagg gcctggttga actgcgcggt gaaaagcaga ttgaaatcaa
    17761 atccggtgca aaaacgtccg tctggctgtt cctgctgggc gtagttggcg tggttatcta
    17821 tgcaatcatc aacagcccaa gcatgggtct ggttgaaaaa ccgctgatga acaccaccaa
    17881 cgcaatcctg atcatcatgc tcagcgttgc aactctgacc accgttatct gtaaagtcga
    17941 taccgacaac atcctcaact ccagcacctt caaagcaggt atgagcgcct gtatttgtat
    18001 cctgggtgtt gcgtggctgg gcgatacttt cgtttccaac aacatcgact ggatcaaaga
    18061 taccgctggt gaagtgattc agggtcatcc gtggctgctg gccgtcatct tcttctttgc
    18121 ttctgctctg ctgtactctc aggctgcaac cgcaaaagca ctgatgccga tggctctggc
    18181 actgaacgtt tcaccgctga ccgctgttgc ttctttcgct gcggtgtctg gtctgttcat
    18241 tctgccgacc tacccgacgc tggttgctgc ggtacagatg gatgacacgg gtactacccg
    18301 tatcggtaaa ttcgtcttca accatccgtt cttcatcccg ggtactctgg gtgttgccct
    18361 ggccgtttgc ttcggcttcg tgctgggtag cttcatgctg taatgatcaa tcgcggggcg
    18421 ttcacgcccc gctttctttc ccgccgacta acatccttcc cccgtacgtt gtatagtgac
    18481 ctctctcttg cggttccatc tgttcttgcg aggtgtttat gcttgatgaa aaaagttcga
    18541 ataccgcgtc tgtcgtggtg ctatgtacgg caccagatga agcgacagcc caggatttag
    18601 ctgccaaagt gctggcggaa aaactggcgg cctgcgcgac cttgatcccc ggcgctacct
    18661 ctctctatta ctgggaaggt aagctggagc aagaatacga agtgcagatg attttaaaaa
    18721 ctaccgtatc tcaccagcag gcactgctgg aatgcctgaa gtctcatcat ccatatcaaa
    18781 ccccggaact tctggtttta cctgttacac acggagacac agattacctc tcatggctca
    18841 acgcatcttt acgctgatcc tgctactttg cagcacttcc gtttttgccg gattattcga
    18901 cgcgccggga cgttcacaat ttgtccccgc ggatcaagcc tttacttttg attttcagca
    18961 aaaccaacat gaccttaatc tgacctggca gatcaaagac ggttactacc tctaccgtaa
    19021 acagatccgc attacgccgg aacacgcgaa aattgccgac gtgcagctgc cgcaaggcgt
    19081 ctggcatgaa gatgagtttt acggcaaaag cgagatttac cgcgatcggc tgacgcttcc
    19141 cgtaaccatc aaccaggcga gtgcgggagc gacgttaact gtcacctacc agggctgtgc
    19201 tgatgccggt ttctgttatc cgccagaaac caaaaccgtt ccgttaagcg aagtggtcgc
    19261 caacaacgca gcgtcacagc ctgtgtctgt ttcgcagcaa gagcagcaca ccgcgcaatt
    19321 gcccttttcc gcgctctggg cgttgttgat cggtattggt atcgccttta cgccatgcgt
    19381 gctgccaatg tacccactga tttctggcat cgtgctgggt ggtaaacagc ggctctccac
    19441 tgccagagca ttgttgctga cctttattta tgtgcagggg atggcactga cttacacggc
    19501 gctgggtctg gtagttgccg ccgcagggtt acagttccag gcggcgctac agcacccata
    19561 cgtgctcatt ggcctcgcca tcgtctttac cttgctggcg atgtcaatgt ttggcttgtt
    19621 tactctacaa ctcccctctt cgctgcaaac acgtctcacg ttgatgagca atcgccaaca
    19681 gggcggctca cctggcggtg tgtttgttat gggggcgatt gccggactta tctgttcacc
    19741 atgcaccacc gcaccgctta gcgcgattct gctgtatatc gcccaaagcg ggaacatgtg
    19801 gctgggcggc ggcacgcttt atctctatgc gttgggcatg ggcctgccgc tgatgctaat
    19861 taccgtcttt ggtaaccgct tgctgccgaa aagcggcccg tggatggaac aagtcaaaac
    19921 cgcgtttggt tttgtgatcc tcgcactgcc ggtcttcctg ctggagcgag tgattggtga
    19981 tgtatgggga ttacgcttgt ggtcggcgct gggtgtcgca ttctttggct gggcctttat
    20041 caccagccta caggctaaac gcggctggat gcgcgtggtg caaataatcc tgctggcagc
    20101 ggcattggtt agcgtgcgcc cacttcagga ttgggcattt ggtgcgacgc ataccgcgca
    20161 aactcagacg catctcaact ttacacaaat caaaacggta gatgagttaa atcatgcgct
    20221 cgttgaagcc aaaggcaaac cggtgatgtt agatctttat gccgactggt gcgtcgcctg
    20281 taaagagttt gagaaataca ccttcagcga cccgcaggtg caaaaagcgt tagcagacac
    20341 ggtcttactt caggccaaca tcacggccaa cgacgcacaa gatgtggcgc tgttaaagca
    20401 tcttaatgtc cttggcctac cgacaattct cttttttgac ggacaaggcc aggagcatcc
    20461 acaagcacgc gtcacgggct ttatggatgc tgaaaccttc agcgcacatt tgcgcgatcg
    20521 ccaaccgtga acgacacttg cagcgggaaa gacggaggaa aatagcgtgc aacgtgaaga
    20581 tgtactggga gaagccctga aattattaga attacaaggg attgccaaca ccacgctgga
    20641 gatggttgct gaacgtgtgg attatccact ggacgagcta cgccgcttct ggccagacaa
    20701 agaggcaatc ctctacgatg cgctgcgtta tcttagccaa cagatagatg tctggcgtcg
    20761 tcagctgatg ctggacgaaa cgcaaaccgc cgaacaaaag cagctggcac gttatcaggc
    20821 gttatcggag tgcgttaaaa acaaccgcta tccgggctgt ctatttatcg ctgcctgtac
    20881 gttttatccc gatcctggcc accctattca tcaactggcc gatcagcaaa aaagcgcggc
    20941 ctacgatttc acccacgaac tgttaaccac actggaagtt gacgatccgg cgatggtagc
    21001 aaagcagatg gaactggtgc tggaaggctg tttaagccga atgctggtga atcgtagcca
    21061 ggcggatgtc gacactgcac atcggctggc ggaagatatc ctgcgcttcg cccgctgccg
    21121 ccagggtggt gcattgacct gacagaaaca cagaaaagaa gcgatttgcc gcaatcttaa
    21181 gcagttgaat cgcttttact gaaattaggt tgacgagatg tgcagattac ggtttaatgc
    21241 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatc
//
LOCUS       DAJTYD010000055        20795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTYD010000055 DAJTYD010000000
VERSION     DAJTYD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.055"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1323)
                     /gene="guaA"
                     /locus_tag="OO585_001870"
     CDS             complement(<1..1323)
                     /gene="guaA"
                     /locus_tag="OO585_001870"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6629482.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLY"
     gene            complement(1392..2858)
                     /gene="guaB"
                     /locus_tag="OO585_001871"
     CDS             complement(1392..2858)
                     /gene="guaB"
                     /locus_tag="OO585_001871"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6629483.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            3020..4390
                     /gene="xseA"
                     /locus_tag="OO585_001872"
     CDS             3020..4390
                     /gene="xseA"
                     /locus_tag="OO585_001872"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6629484.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(4387..4602)
                     /locus_tag="OO585_001873"
     CDS             complement(4387..4602)
                     /locus_tag="OO585_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6629485.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(4671..6143)
                     /gene="der"
                     /locus_tag="OO585_001874"
     CDS             complement(4671..6143)
                     /gene="der"
                     /locus_tag="OO585_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6629486.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(6261..7439)
                     /gene="bamB"
                     /locus_tag="OO585_001875"
     CDS             complement(6261..7439)
                     /gene="bamB"
                     /locus_tag="OO585_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6629487.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(7450..8070)
                     /gene="yfgM"
                     /locus_tag="OO585_001876"
     CDS             complement(7450..8070)
                     /gene="yfgM"
                     /locus_tag="OO585_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6629488.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(8088..9362)
                     /gene="hisS"
                     /locus_tag="OO585_001877"
     CDS             complement(8088..9362)
                     /gene="hisS"
                     /locus_tag="OO585_001877"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6629489.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(9473..10591)
                     /gene="ispG"
                     /locus_tag="OO585_001878"
                     /gene_synonym="gcpE"
     CDS             complement(9473..10591)
                     /gene="ispG"
                     /locus_tag="OO585_001878"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6629490.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(10618..11631)
                     /gene="rodZ"
                     /locus_tag="OO585_001879"
     CDS             complement(10618..11631)
                     /gene="rodZ"
                     /locus_tag="OO585_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6629491.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(11916..13070)
                     /gene="trmG/rlmN"
                     /locus_tag="OO585_001880"
     CDS             complement(11916..13070)
                     /gene="trmG/rlmN"
                     /locus_tag="OO585_001880"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6629492.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(13220..13651)
                     /gene="ndk"
                     /locus_tag="OO585_001881"
     CDS             complement(13220..13651)
                     /gene="ndk"
                     /locus_tag="OO585_001881"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6629493.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(13800..16112)
                     /gene="pbpC"
                     /locus_tag="OO585_001882"
     CDS             complement(13800..16112)
                     /gene="pbpC"
                     /locus_tag="OO585_001882"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6629494.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(16113..>20795)
                     /gene="yfhM"
                     /locus_tag="OO585_001883"
     CDS             complement(16113..>20795)
                     /gene="yfhM"
                     /locus_tag="OO585_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6629495.1"
                     /translation="DQDFSRVIHVVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIV
                     TIGKEVKALNNATFSKDYEKTITTRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNV
                     DVNFFRVKPESLPAFISQWEYRNSLANWQSDKLLQMADLVYTGRFDLNPARNTREKLL
                     LPLGDIKPLQQAGVYLAVMNQAGRYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLE
                     NGAAQQGIEVSLLNEKGQTLTQATSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLP
                     ALDLAEFNIAGAPGYSKQFFMFGPRDLYRPGETVILNGLLRDADGKALPDQPIKLDVI
                     KPDGQVLRSVVSQPENGLYHFTWPLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPE
                     RMALNLTGEKTPLTPNDEVKFSVVGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFE
                     FGDIAAENLSRTLDEVQLTLDDKGRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVT
                     RRAEQAIWPANALPGLRPQFALKSVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVK
                     KAVSGLQVRLIRERRDYYWNWSEDEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVE
                     WGAYRLEVKAPNEAVSSVRFWAGYSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKL
                     HIAAPTAGKGYAMVESSEGPLWWQEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPS
                     DKSRSATPKRAVGVLHLPLGDENRRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQ
                     VNVLVSAVDSGVLNITDYVTPDPWQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGG
                     DGDELKRGGKPLVNHVNIVAQQALPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADD
                     FGSNESKVIVAAPVIAELNMPRFMASGDTSRLTLDITNLTDKPQKLNVALTASGLLEL
                     VSDSPAAVELAPGVRTTLFIPVRALPGYGDGEIQATISGLALPGETVADQHKQWKIGV
                     RPAFPAQTVNYGTALQPGETWAIPADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELK
                     AYPYGCLEQTASGLFPSLYTNAAQLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGG
                     FALWDKNGDEEYWLTAYVMDFLVRAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPY
                     ADNLKASKFAVQSYAALVLARQQKAPLGALREIWEHRADAASGLPLLQLGIALKTMGD
                     ATRGEEAIVLALKTPRNSDERIWLGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTL
                     SQQAFGERWLSTQESNALFLAARTIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQL
                     ATLQVRNSGDQPLWLRMDASGYPQSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDL
                     VLVWLQVKASNSVPDALVVDLLPAGLELENQNLANGSASLEQSGDEVQNLLNQMQQAS
                     IKHIEFRDDRFVAAVAVDEYQPVTLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAA
                     EDLLIVRP"
BASE COUNT         4870 a         5893 c         5146 g         4886 t
ORIGIN      
        1 gtacaggtcc gctttacgca gttcttcaat gaagatggcg tcagcacggc gcagcaggtc
       61 acagtactct ttcttcactt cacccagcac acgaacgcca aggcctggtc ccgggaacgg
      121 gtgacggtac agcatgtcgt acggcaggcc cagctccaga ccaatcttac gcacttcgtc
      181 tttgaacagc tctttcagcg gttcaaccag gcccatcttc atctctttcg gcaggccgcc
      241 cacgttgtgg tgagatttga tgacgtgtgc tttaccggtt gcagaagccg cagattcgat
      301 aacgtcaggg tagatggtgc cctgcgccag ccacttcacg tcttccagtt tcagcgcttc
      361 ttcatcgaac acttcaacga aaacgcgccc gatgatttta cgttttgctt ccggatcgtt
      421 ttcgccagcc agcgctgaca ggaagcgatc ttctgccggt acgtgaacaa tgttcagacc
      481 aaagtgatcg ccaaacatat ccagaacctg ctctgcttcg ttgagacgca gcaggccgtt
      541 gtcgacgaat acgcaagtca gatttttacc gatagcgcgg tgcagcagca ttgcggttac
      601 ggaggaatcc acaccaccag agaggccgag gatgacttta tcgtcgccta cctgctcgcg
      661 gatgcgagct acagcatcgt cgataatttt cgctggcgtc cacagggctt cacactggca
      721 gatatcacgc acaaaacgct ccagcatgtg cataccctgg cgggtgtgag tcacttccgg
      781 gtggaactgt acgccataga agcgtttttc ttcgttagcc ataatggcaa acgggcagct
      841 ttcggtgctg gctacggtga cgaagtcgga cgggatagcg gtaactttat cgccgtggct
      901 catccagaca tcgagcagcg gtttaccgtc tgcggtcagc gcatcttcga taccgcgaac
      961 cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca aattcacgtt cgttagaggc
     1021 ttcaacgtga ccgcccaact gcattgccat ggtctgcatg ccatagcaaa cgccgaatac
     1081 cggtacgcct gcttcaaaga catactgcgg cgcacgcgga ctgttttctt cagtagtact
     1141 ttccgggccg ccggaaagaa taatgccgct tggattgaag tcacgaattt gtgcttctgt
     1201 cacatcccac gcccacagtt cgcagtaaac acccagctca cgcacgcggc gcgcaaccag
     1261 ttgagtgtac tgagaaccga agtccagaat gaggatgcga tgcttatgaa tgttttccgt
     1321 cattgacgct tattccgagg caagtgaaac agataatata aatcgcccga catgaagtcg
     1381 ggcgaagaga atcaggagcc cagacggtag ttcggggact ctttagtaat ggtcacgtcg
     1441 tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta caaactccgc tttagtacgc
     1501 agttcgtcga tagtaccaca gccggtcagg cccatacagg agcgcaggcc gcccatctgc
     1561 tggtgaatga tctctttcag gcgaccttta taggctacgc gaccttcgat accttccggc
     1621 accagtttgt cggcagcgtt gtcactctgg aagtaacggt cagaagaacc tttggacatc
     1681 gcacccagag agcccatacc acggtaagat ttgtaagaac ggccctggta gagttcaatt
     1741 tcacccggag attcttcagt acccgccagc atggaaccca ccattaccgc gcttgcgcca
     1801 gcggcgatag ctttggcgat gtcgccggag aagcgaatac caccatctgc gataaccgga
     1861 atacctgtgc cttccagcgc ttcaactgcg tcagcaacgg cagtgatctg cggaacacct
     1921 acgccagtaa cgatacgagt cgtacagata gaaccagggc cgataccgac tttaaccgcg
     1981 ctgcaaccag cttctgccaa agcgcgtgca cctgcagctg ttgccacgtt gccgccgatg
     2041 atttgcagat ccggatattt agcacgggtt tcacggatac gctgcaaaac gccttctgag
     2101 tggccgtggg aggagtcgat cagcagaacg tcaacgccag cggcaaccag cgcgtcaaca
     2161 cgctcttcgt tacccgcacc tgcgccaact gcagcaccaa cacgcagacg gccttgctcg
     2221 tctttacagg cgttcggttt acgttccgct ttctggaagt ctttcacggt gatcatgccg
     2281 atcaggtgga attcgtcatc aaccaccagc gctttttcaa cgcgtttttc gtgcattttt
     2341 gccagcacca cttcacgggc ttcaccttca cgcacggtga ccagacgctc tttcggcgtc
     2401 atgtaaacgc taaccggctg gttcaggtcg gtaacaaaac gcacgtcacg accggtgata
     2461 atacccacca gttcgttttc ttcggtaaca accggatagc ccgcaaaacc gttacgctcg
     2521 gtcagttctt tcacttcgcg cagcgtcgtg gttggcagaa cagtctgcgg atcagtcacc
     2581 acaccagatt cgtgtttttt cacacggcga acttcttctg cctggcgttc aatggacatg
     2641 tttttgtgga taaagccgat accgccttcc tgagccagag caatagccag gcgcgcttcc
     2701 gttacggtat ccattgctgc ggaaagcata gggatattca gacgaatagt tttcgtcagc
     2761 tgggtgctga ggtcagcagt attcggcaga acggtagaat gagcaggaac gaggagaacg
     2821 tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg gcaatatctc gaccagagtg
     2881 gttaataaat attgccgcgg cattatacag agcgtaaccg attgcatcta cccctttttg
     2941 caaaaaatgc ttgctatccc cgaagggcgg gttactatcg actgaataac ctgctgattt
     3001 agaatttgat ctcgctcaca tgttaccttc tcaatcccct gcaattttta ccgttagtcg
     3061 cctgaatcaa acggttcgtc tgctgcttga gcatgagatg ggacaggttt ggatcagcgg
     3121 cgaaatctct aatttcacac aaccggcttc cggtcactgg tactttacac tcaaagacga
     3181 caccgcccag gtacgctgcg cgatgttccg caacagcaac cgccgggtga ccttccgtcc
     3241 acagcatgga caacaagttt tagttcgcgc caatattacg ctctacgagc cgcgcggtga
     3301 ctaccagata atcgttgaga gtatgcagcc ggccggtgaa gggctgctgc aacagaagta
     3361 cgaacagctc aaagcgaagt tgcaggctga aggtttgttc gatcagcaat acaaaaaacc
     3421 acttccctcc cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg ctgcgctaca
     3481 tgatattttg catgtgttaa aacgtcgcga tccttctcta ccggtgatca tctaccccac
     3541 cgccgttcag ggcgatgacg caccggggca aattgttcgc gccattgaac tggcgaatca
     3601 gcgcaatgag tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg aagatttatg
     3661 gagttttaac gacgaacgcg tagcgcgggc gatttttgcc agccgcattc cggtcgtcag
     3721 cgccgtcggg catgagacgg atgtgaccat tgccgatttt gttgccgatc tgcgtgcgcc
     3781 aacgccgtct gccgccgctg aagtagtgag ccgtaatcag caagagttac tgcgccaggt
     3841 gcaatcgacc cgtcaacggc tggagatggc gatggattat tatctcgcca accgcacgcg
     3901 tcgctttacg cagatccatc accgattaca gcaacagcat ccgcagctcc ggctggcacg
     3961 ccagcaaacc atgcttgagc gcctgcaaaa gcggatgagc tttgcgctgg aaaatcaact
     4021 taagcgtacc gggcaacagc agcagcggtt aacacagcgg ctgaatcagc aaaatccaca
     4081 gccgaagatt catcgcgcgc aaacgcgcat tcagcaactg gaatatcgtt tagcagaaac
     4141 cctgcgcgta cagcttagcg ccacgcgtga acgtttcggt aatgcagtaa cgcacctcga
     4201 agccgtaagc ccactgtcaa cgctggcgcg tggatacagc gttactactg ctactgacgg
     4261 caatgtactg aaaaaagtga agcaagttaa agcgggtgaa atgctaacca cacgtctgga
     4321 agacggctgg atagaaagtg aagttaaaaa catccagcca gtaaaaaaat cgcgtaaaaa
     4381 ggtgcattaa gccagcacga attcaacgcg ttttttcgat atcaagccat gcccgtgctg
     4441 acagaagtaa tctactgcac cgcaggcttt taacacctgc aacggttgat ggcagtctgg
     4501 acaaagagct ttcatttcaa taatttcccc gcaggatggg caacgcgcat ggccgttatc
     4561 ctgatcaagc acatgctgac attgtggaca atgcagttcc atatgaattc ctccggtagc
     4621 catccctcta cattcataga gggaatggca gataaaatac ttacggataa ttatttattt
     4681 ttcttgatgt gcttcatcag acgcttacgt ttacgcatct gggttggcgt cagggtgtta
     4741 cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg cgatcccatt
     4801 acgtccagcg atttgcggaa gtagttcatc aagtagcgct tgtaggaatc aggcaggtct
     4861 ttcacctgat taccgtgaat caccacaatc ggcgggttat aaccaccagc gtgggcatat
     4921 ttcagcttca cacgacgacc gcgtaccagc ggcggttggt gatcttcaac agccatcgtc
     4981 atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata cgcttcacgt
     5041 actgattcaa acaagttacc aacaccactg ccgtgcaagg cagagataaa gtgcacacga
     5101 gcaaaatcga taaagcccag acggaagtcc agcgtttctt tcacctgctc tttcacttcc
     5161 tggctcaggc catcccactt attcaccaca atgacaagtg agcgcccact attgagaata
     5221 aagcccagca gcgagagatc ctgatcggaa ataccttcgc gcgcatcaat cactaacatc
     5281 accacgttgg catcttcaat ggcctgcaac gttttgatta cggagaattt ctctacagca
     5341 tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata ctcacgtcca
     5401 tcgcgttcca ttggaatgta gatactgtca cgcgtcgtgc caggcatgtc ataaacaaca
     5461 acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt cggacgaccc
     5521 acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc ttcttcttcg
     5581 ccgttctctt ccacttcaaa ttgcgcccag tattcagcgt cttcgtcgac ttcctcttgc
     5641 ggtgcgagat cttccatcca cggcagcagc acatgctcca gcagacttaa tacgccacga
     5701 ccgtgagacg cggcgatcgg gtagatttca cctaagccaa gcgagtagaa atcaaccact
     5761 gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg tttttcacgg
     5821 gagcgcagat gtttggcaat cgcttcatct gccggcatca ggcccgcgcg cgcatccacc
     5881 ataaacagta cgacgtccgc ttcttcaatc gccagcagcg actgttccgc catgcgggtt
     5941 tctacaccgt cttctgtgcc atcaatcccg ccggtatcaa tacagataaa ctcacggcct
     6001 tcaatttccg cacgaccgta cttacggtca cgagtcagac ccgggaaatc cgcaaccagc
     6061 gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt agggcgcccg
     6121 acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat cagacaacgc
     6181 acgctatatt cgcgtcgttg ttaaaaacag gaaaacggcc cctgtccagg agccgttttc
     6241 aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc ttttgcctgg
     6301 atcagcagtt tgccgtcagc ggcaaccggt tcagtctgga aaccggaact atcaactttt
     6361 tgctgggcaa cgaaacgacc atcttcgaca ttaatccagt gcagataacc ttcactgtca
     6421 ccgaccacca ggttgccatc atacagcacc ggagaagtca gcaggcgatg caacagatcg
     6481 ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg gtcattttga
     6541 tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc cagttcgcgt
     6601 ttccacataa tctgaccact gcgcagatca agcgccgtca ggttaccatt ataggccagc
     6661 gcgaaaacaa cgccgttaac aacgacggga gtcgtgtcaa catcgctcag acggtcaatt
     6721 tcggtagaac cggtcgcctg ggaaatacgc tgctgccaaa tcatctggcc ctgttccatc
     6781 agcactgcgc tgacgcgacc attatcgccc cccacgacgg ccgcaccaaa agccgttgcc
     6841 ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt ccatttgaca
     6901 gcgccgtcag cttcgttcag cgcttgtaac tgaccgttac tggtgtggat taacaccaga
     6961 ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg ccatgccaca
     7021 gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cgctgccaat gtagacatgc
     7081 ccaccagaca cggtcacacc gccagaaagt aatgcaggct ctttagagaa ccagccatct
     7141 ttctcggcca ggctgacaga ccagatttct ttgccatcat ccgcattcag cgcttttacc
     7201 aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg aagattggaa
     7261 tagaagttgc caatgccgct accaacggaa gtactccacg ccgtggtcgg cgtaaactgg
     7321 ttttcaacgg ttggcaatgg ggacatcttt accacatctt cttcgctgtt aaacagcgaa
     7381 cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg caattgcatc
     7441 gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc tcagtgccgg
     7501 agtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct tatcaccttt
     7561 gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt cacctttgat
     7621 ggtatcaagg gttttcagcg cggcatcagc ctgcttgagc tgtacctgaa cgcgagcaag
     7681 acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc cctgttgtaa
     7741 ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa gttccaaaga
     7801 agccagcgca ccataagtat ttttattttc agcagcaaat ttttccgccg ccgggatgct
     7861 atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag aagcggagcg
     7921 tgcagaatca acctgatggc tgttccagta gcgccagcca atcagtgcgc caacgcccaa
     7981 aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa ccgcttctac
     8041 ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta acccagtaac
     8101 gtgcgcaaat gcgcggctac gctatcctgc gcaactgccg tttgctcacc agagcgcaaa
     8161 tccttcacta ctgctgtgcc gttagtcact tcagactcac ccagcaccac agcaacgcgg
     8221 gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc gtggttggtc
     8281 atcaatttca cgccctgtaa ttcatcacgc agacgctcag ctaatgccat agccgcagat
     8341 tgtgtatcag cacctgaagc caccaggtat atatcgacaa taggatcggc tttaaattcc
     8401 ggattaacgg cctgtactaa caatacaaga cgttcgaggc ccatcgcaaa accgacagcc
     8461 ggtgttgcac gaccgcccag ttgttccaca agaccgtcat aacgaccgcc tgcacacacg
     8521 gtgccctggg agccgagact gttagtcacc cactcgaaaa cggtacggtt atagtaatcc
     8581 agaccacgca ccagacgctg gtttacggtg taagcgatcc ccgcgctctc aagcagtttg
     8641 cacagaccgg caaaatgctc acgagattcc tcgtccagat aatcacctaa tgccggagcg
     8701 tcgttgagaa gcgcctgcac ttccggattt tttgaatcca gcacgcgcag cgggttagtg
     8761 tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag gaatgccacc
     8821 agcgcatcgc ggtaattggc gcgtgcttcc agcgaaccga tagaattcag ctcaagagtt
     8881 acgtgttcgg aaatacccag cgcgcgccac cagcgggcgg tgagcataat cagttcagcg
     8941 tcgatatccg gaccttgcag accgaaaact tcgcagccca actgatggaa ctgacgataa
     9001 cgccctttct gcggacgctc gtgacggaac atcggcccga tataccacag acgctgttcc
     9061 tgattgtaca gaagaccatg ctcgatgccg gcgcgtacac agcccgccgt cccttcaggg
     9121 cgcagagtca ggctgtcgcc attgagatcc tcaaaggtgt acatctcttt ttcaaccacg
     9181 tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat cggcaagcgg
     9241 atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat gcgctgccag
     9301 atggccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat gttttttgcc
     9361 acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca atgtttgggg
     9421 agccatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt tattattttt
     9481 caacctgctg aacgtcaatt cgacgcgctt catccagctg actggctttc gcacgaatgc
     9541 gtgcttccag ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc acgccatctt
     9601 catagaggcc gcttttcttg ttgccgccgg tgacgccgag tgtagaaacc agcgcctcac
     9661 ctgggccatt caccacgcag ccgataatag aaacgtccat cggagtgatg atatcttcca
     9721 ggcgttgctc cagcgcgtta accgtaccga taacatcaaa ttcctgacgc gaacaggtcg
     9781 ggcaggcgat gaagttgatc cctcgcgaac ggatacgcag cgatttcaaa atatcgaaac
     9841 cgactttgat ctcttcgacc ggatcggccg ccagcgatac gcgcagcgtg tcgccgatgc
     9901 cttcagacag cagcagacct aaaccaatgg cggattttac tgccccgctg cgtgcaccac
     9961 cggcttcggt gatccccaga tgcagcggct gatcgatctg ttttgccagc aaacgataag
    10021 actcaacagc gaggaagacg tcagacgctt tcacgctgac tttgaactga tcgaagttca
    10081 ggcgatcgag atgatcaaca tgacgcatgg cggattccag caacgcctgc ggcgtcggtt
    10141 cgccatactt ttcttgcaga tctttttcca gcgatccggc gttaacgcca atacggatcg
    10201 gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca ttaccgatat
    10261 tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagc gcaatgcgat
    10321 agtcgaagtg gatgtcagcc accagcggca cgttaacccg ctgtttgatg agtttgaacg
    10381 cttctgccgc gtccatcgtc ggtacggaga cacggacgat atcagcgcca acgcgttcca
    10441 gcgccttgat ttgattgacc gttgcttcga catctgtcgt acgcgtattg gtcatggact
    10501 gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata cgtgttgatt
    10561 ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac ccgtctgtta
    10621 ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag ttctgataaa
    10681 acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg gcgcaccaat
    10741 tttcagtttg tacggtgcct ggccggttaa gttcaaatta ccgtctttac gctgcatacc
    10801 gctaaacaat tttttaccgg tagcatcagt gacctccagc cagcaatcgg cagtaaagtt
    10861 catcaccagc gcattcggat cagccaccgg cgtggtcacg ccagcctgat cggttggcaa
    10921 cggcgcagca ccatctggcg ttgttgctgc cgttggtgcc ggggtcgcgg cggtatcaac
    10981 atttgcctgc gaaggcgaaa caaccgcatt ctgttgcgga tcaacagctg gtgctggcgc
    11041 agttacggca ggtgtttgcg tgttggttgc ggtagtatcc acagacgctg gcggcgtgga
    11101 cgttgtagcc gggtctgtag ttgtcgacgt atttaacgga acactctgcc cctgctcgct
    11161 attactgctc agttccgccg aagattgatc ggccatagtg gtgatctctt cctgctgagc
    11221 tttgtggtct tgccaccacc aggcaccgct caggccgata accacaaaca acaccagcca
    11281 ggtgaaggtc atcagccagc cgtcacgttt tttgcggcgt ttaccgaggg aaaaactctg
    11341 catcggcgca actttcgcag cccgtagtgg agcctgcttt tccagcccta gcagcagttc
    11401 ttcttctgga atatgtacca gacgcgcata agagcggata tatccgcgca ggaatgttga
    11461 agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa ccttcaggca
    11521 aagtcgctcg gcaacggcct gctgactaag tcctagttgt tcgcgagcat tacgcaggcg
    11581 agcgccggta gtaagtgctt cattttggtc gtgcgtggct tcagtattca ttcgctgcag
    11641 gtacgtttaa atgagaattg agatgccggt gaaccatcat gctcacccac accgcgaaac
    11701 atccgttaag ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg tgacaaagcc
    11761 cacattcact aaggctaact tactgttgca tcgttacata ctgccttaaa gtcagcaaaa
    11821 acgcaccgtt aatattgacc agacaattgc aacttaatac ttcattcata aaagattacg
    11881 ccacggtaca taaagtaacc gtggcgtaat ggctatcaga ccgctttaat gtcgatggct
    11941 tcaccctgca tccgtttacg cagggtacgt ttcgtacggt cgataacatc gcccgccaac
    12001 tgaccacagg cagcatcgat atcatcacca cgagttttac gcacaatagt ggtgaaaccg
    12061 tagctcatca gcacttttga gaaacggtcg atacggctgt tcgaactgcg tccatacggc
    12121 gcgcccggga acgggttcca cgggatcagg ttgatcttac acggcgtatc tttcagcagc
    12181 tccgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat cacgtattca
    12241 atagtgactc ggccctgatt ggcgttggat ttctccagat aacggcgcac cgcagcaagg
    12301 aacgtttcga tattgtactt tttgttgatc ggcacaattt cgtcacgaat ttcgtcgttc
    12361 ggcgcgtgca gggaaattgc cagtgcaacg tcgatcatat cgcccagttt atccagcgcc
    12421 ggaactacac cggaagtaga aagcgtgacg cgacgtttag acaggccaaa accgaaatca
    12481 tccagcatga tttccatcgc cggaacgacg ttgttcaggt tgagcagcgg ctcgcccatg
    12541 cccatcatca ctacgttagt gattgggcgc tgaccggtga cttttgctgc gccgacgatt
    12601 ttcgccgcac gccacacctg gccgataatt tccgacaccc gcaggttgcg attaaagccc
    12661 tgctgggcgg tggaacagaa tttacactcc agcgcacacc ccacctgcga agagacgcag
    12721 agcgtggcac ggtcgtcttc cgggatatac accgtttcga cgcgctgatc gccaacggcg
    12781 atcgcccatt taatggtgcc gtcggatgaa cgctgttctt caaccacttc cggtgcgcgg
    12841 atttccgcca cctctttcag tttgccgcgc aacactttgt tgatgtcggt catctcatca
    12901 aagttgtcgc agcaatagtg atacatccac ttcatcacct gatcggcgcg gaagggtttt
    12961 tcacctaaat ctttaaaaaa ctcccgcatc tgctgacggt tgagatccag caggttgatt
    13021 tttccatctt tcgtggtgac gttttcaggt gtgactaatt gttcagacat atgctattcc
    13081 ggcctcgtta ttacacgtta tggcccctgg agggttgaaa aaagaaacgc cccggtgagc
    13141 ggctgctcgt ccgggggcgc tgcattgtac aaattctggc gcacggatgc cacgtttgca
    13201 cgcggcattt acgaaattat taacgggtgc gcgggcacac ttcgccttcg ccaaagaaat
    13261 aagcgatttc gcgagcggca gattcgacgg aatcagaacc gtgggtaccg ttttcggtca
    13321 ggctgtcagc gtaatcagcg cgcagagtac cagccagtgc gtttgccgga ttggtcgcgc
    13381 ccagcagatc gcggtgacgc tgaacggcgt tttcaccttc cagcacggaa accacgatcg
    13441 ggccagaggt catgaattca accagaccat caaagaacgg ttttccatcg tgttcagcat
    13501 aaaagccacg tgcctgttca acggtcaggt gcagcatttt ggtgccgaca attttgaacc
    13561 ctgcagcttc aaagcgcgca aagatattac caatgacgtt ttttgctacc gcgttcggtt
    13621 tgatgatgga aaaagtacgt tcaatagcca tttttacctc tgtaaattgt tctgttgttg
    13681 tctgtaccag cgtacgagat ggcgcggatt ataatgagca acagggccgt tgactattga
    13741 tgaaggtaac atttttttaa aataaaatga ggtttagcaa caatcaggga caaaacagac
    13801 tattgcatga caaatttcac tgtcgcgatt tgccccacgt catccatcac cagcaattga
    13861 taatcacctt tatccgtcaa atgcagggtc acgttgcgcc cgcgttcagt taacggttcg
    13921 ccattcagaa accaccagcg ttcacctgcc cctccacttg attgcaacgg caaagtcgct
    13981 tccgctgcgc ccggtaaacg tttaataatc gcgccatcgc gaacgcctgt cagctgcagc
    14041 gggagctggg catagtgacc gtatggcggg caacttgtcg aggccggtgg taagcgcaca
    14101 gcgcgacgtt ctgatgcggg cagccagggt tccagcggta gcggccagac atttatcatt
    14161 tcttgtcgcg cttgcgggca atcagcggca acacgtttgc cattttcatc cagccagatg
    14221 gggaaacgaa tgccattaat gccttcctgc tccggcagta atagagttgg cggctgactc
    14281 ccgtccagca gccaggttgc caggcggcgg cgacagttac cgtcaccttc cggcaaagac
    14341 tgtccgcccg gccagcagat aacgccacga gtgactgagt tcgggcgcgg gtcttccggc
    14401 agattcgcac tgcgcgacag taagatatta ttgacctgat tcaacaatgg tacggcactg
    14461 gcaaagccaa actgaccaac aacgggcgtg ccgtccggtc tgccagtcca gatcccaatg
    14521 acatagcgag cgttaacccc aatcgcccag gcgtcacgat agccatagct ggtgcccgtt
    14581 ttccatgcca gtggggcgac gcgcggcaag gcactatccg gcaagggttg cgcttcatca
    14641 gccataatcc ggcgaatgat ccacgccgcc cccgacgaca ttaaaggcca ttcaagcagc
    14701 ggatcgtcag gctgtaagcg caatttgcct gccttgccgt ggcgagcaaa cgcggtatac
    14761 gctgccgcca tatcttccag ttttgcacca gcgccgccga gaatgagtga aagattcggc
    14821 gcagcaccgt tgggcaaata taacggcaat ccaacattgc gtaactttgc cgcaaaccgt
    14881 ttcggtccat aggcttccag cacctgcaca gcaggtaagt tcagcgagcg caccagcgcc
    14941 tcgctcatgc tgatcgggcc atgaaaaccg ctatcaaagt tacctggtcg ataatcaccg
    15001 gtgcgccggg ggacgtcttg cagcagtgat gccgggtgga tcaagccttc atccagcgcc
    15061 agaccataaa caaacggttt gagcaccgat cctggcgatc ggatcgcatt gaccatatca
    15121 acatgaccaa agcgtgaatc atcgttgaga tcaaccgatc ccacccagcc gcgaacacgc
    15181 atatcggtat gatcaaccac gatcatcgcc agtgagctgc gcggtggcaa ccgccctttc
    15241 cagttttgcg ccagttcttc cagacgtcgt tgaagaccgg catccagcgt agtaacgatt
    15301 ttgtcgcttt tgcttttacc gagcatcatg cgcgaaaaca gcggtgccag ttgcggcatt
    15361 tgtcggggtg ccagccagat gggttcttcc cttgactctt ttacctgctc acgggaccac
    15421 acaccttgca cggccatccg ttcgagcact ttattacgcg cggcttcggc acgctccggc
    15481 caacgatccg ggcgcagtcg gctgggcgct tgcggtaaca ccgccagcat tgccgcctcg
    15541 gaatagctta aattcgcagg cgattttccg agataagccc aacttgccgc accgatcccc
    15601 tgcaacgtac cgccaaacgg agcgcggtta agatacaaga tcagaatttc acgcttagac
    15661 aaatgccact ccagttgcaa cgcgcgccag agctggagaa ttttgccacc aaatgttttg
    15721 gggtgaggat caagcagacg agcaacctgc atagtgagcg tgctgccacc agaaataacc
    15781 cgtcccgaag tgagatcttg ccatgctgcg cgcgccaccg agaatggatt cacccccgga
    15841 tgcttccaga accagcgatc ttcataattg atcagcgctt caaggtaacg tggagacaca
    15901 tcttcgattg ttaccggata acgccagatg ccatcagcat cggcgaagcg ccagagcggc
    15961 gtaccatcct gcgccacgac cactcgtgcg ggattgactt catgcagcgg tagaggccag
    16021 agtttatctg ctccccacgc tgcaagaaaa aggagaaatg gcacagctgc cagcgttatc
    16081 cagcagccgc gtttggttaa caagcgaagc atttacggtc ggacaatcag cagatcttca
    16141 gccgcgccgg tcgcccgcca ttggggaaca tacattgatt ccaccatcgg ttgcggtacc
    16201 tgatacgttc ccggcgtcac cgcccgcgcc agatacacca gcgttaccgg ttggtattca
    16261 tcaacggcaa ccgccgccac aaagcgatcg tcacggaact caatgtgctt aatgctcgcc
    16321 tgctgcatct ggttcagtaa gttttgcact tcgtcaccac tttgctccag gctggcgcta
    16381 ccgttcgcca gattctgatt ttccagttcc agacccgcag gcagcagatc cacgactaac
    16441 gcatccggca cgctgttact ggcttttacc tgcaaccaga ccagcaccag atcgccgcta
    16501 cgtaacgagt ccagcgattt gctcttacca tcagtaccaa gaatatgacg ctcgatttgc
    16561 agcacattgt tcgccggtaa aggtgcggat tgcggataac cgctggcatc catacgcaac
    16621 cataacggct gatcgccact gtttctcacc tgcaaggtgg caagttgatc gctattcaga
    16681 ttgctgttta gcgttttctc acctgtcagc ggctcagctg agaaagaggt ttgcgcctgc
    16741 catttacccg gtaaatcctg aatcgtacgg gcagccagga acaacgcgtt actttcctgc
    16801 gtcgatagcc agcgttcacc aaacgcctgc tgcgaaagtg tgttcagcaa agtgtactgc
    16861 tcatcgggta gcagtttatt ttcttccagc aaggagagca ttaacgcgtt gtcgcgcagt
    16921 ggactaccgt aatcacccag ccatatccgc tcatcactat ttctcggcgt tttcagcgcc
    16981 agcacaatcg cttcttcacc acgcgttgca tcacccatgg ttttcagcgc aatgccaagt
    17041 tgcagcagcg gtaaaccaga agcggcatct gcacgatgct cccagatttc acgcagcgca
    17101 cccagtggtg ctttttgctg acgggccagc accagcgcag cgtaagactg aacggcgaat
    17161 ttactggctt tgagattatc cgcgtacggg atcgacatca tgcccggatc ttgtaaatag
    17221 cgcagcagac gctcattacc ccggttaatg gcgtctgtcg gcacgctgta accctgttcg
    17281 cctgcgcgga ccaggaaatc catcacgtaa gccgtcagcc agtactcttc gtcaccgttt
    17341 ttatcccaca gcgcaaagcc gccgttatca cgttgcattt gcagcaaacg ggaaatgccg
    17401 atatcgaccg atgcacggcg tttctcatca ctgtcgcctt tgatgcccaa cgcctgcagt
    17461 tgggctgcgt tggtataaag tgacgggaac aggccgctgg cggtttgctc aagacagccg
    17521 tacggatacg cttttaactc tttgatataa cgtgcaatgt tcagcggtgg tttgccgctc
    17581 aacaacaatt gcccttccag cgtaacaggc gagaagtttt gcaatccatc cgccggaatc
    17641 gcccatgtct caccaggctg taacgccgta ccgtaattaa ccgtttgtgc cgggaacgcc
    17701 ggacggacgc cgattttcca ctgcttatgc tgatcggcaa cggtttcacc cggtaacgct
    17761 aacccgctaa tggtggcctg aatttctcca tcgccataac ccggcaatgc tcgcaccggg
    17821 ataaacagcg tagtacgcac acctggcgct aattcaacgg ctgcgggtga atcgctgacc
    17881 agttcaagca aaccactggc ggtcagggca acgttcagtt tttgcggttt atcggtaaga
    17941 ttagtgatat ccagcgtcag acgcgaggta tcgccactcg ccataaagcg cggcatgttc
    18001 agttcagcaa tcaccggtgc ggcaactatc actttgcttt cgttgctacc gaaatcatct
    18061 gccgtccagg cttgcgccat gacgcgcaat tcaccgttaa aatcgccaat cggcagtgta
    18121 accgagcctt cgccctgttc gttgagcgtt accggcagcg cctgctgcgc gacaatattg
    18181 acgtgattga ccagcggttt accaccacgt ttcagctcat caccatcgcc accgaaacgc
    18241 agagctgcca gacgcccctg accttcaata acctgaccgt aaatatcgta aatgtctgcg
    18301 ccatagcgtt tctgaccaaa gaacgcctgc cacggatctg gcgtgacgta gtcagtaata
    18361 ttcagcacac cactatcaac ggcagacacc agcacattca cctgtttagg cttctcgcca
    18421 tttttagtgc tggctttaat tttcacggtt aatggttgat tgggacgcat ttttgctggt
    18481 gtttccagcg ccagatcgag gcgacggttt tcatcgccaa gcggcagatg caacacacca
    18541 accgcgcgtt ttggcgtcgc ggagcgagat ttatcgctag gacgtaccac cagcgtactt
    18601 aaatacagat catgacgatt ccaggtttta tcgaccggaa tcgtcagatc cagcccttga
    18661 gccggaacat caatctcttg ccaccacagc ggcccttcac tggactcgac catcgcataa
    18721 cctttacccg ccgttggcgc ggcgatatgc aacttaatgg tgtcgccagg gcgataactg
    18781 gctttatcca gtttcagcgt gacacggtcg ggtcgcactg cgccgctacc gtcgctgttg
    18841 tcctgccaac tatagccagc ccagaaacga acactactga ccgcttcatt cggcgctttg
    18901 acttccagac gataagcgcc ccactctacc ggaaaactga ccttgccggt ttcgtccgct
    18961 ttcagatcca gagtttgttc attttcgatc agatcttttt gatcaaactg tgactgccag
    19021 ccttcatctt ctgaccagtt ccagtagtaa tcgcggcgtt cgcgaatcag gcgcacctgc
    19081 aagcccgaca cggctttttt cacgccttgt gcatcgctat aaacgatgtc aaaagcggcg
    19141 ttactgcctt catcaacaat gggctgtttc accgtgctgt cagtgcgata atcgtaaacc
    19201 gatttcaagg cgaactgcgg acggagcccc ggcaatgcat tggcaggcca gatagcctgc
    19261 tcagcgcggc gcgtcaccgg gcgaccgccc gattccagca aactgccctg gaaaataacc
    19321 tgcaatgggg aatgcgtttc cttccactgg ctttctgtag aaacttcgcc gcgcccttta
    19381 tcatccagcg tcaactgaac ttcatccagc gtgcgggaaa ggttttcggc agctatatcg
    19441 ccgaattcaa aaccaggtaa ggctgacaca gcctcacgca gtgggcgcag gaaaagttgc
    19501 ccttgcaaag tattaccatt agcaggcgca ccgtacaggt agtatcccac cacggagaat
    19561 ttcacttcat cattcggcgt aagcggggtt ttctcaccgg tcagattcag cgccatacgc
    19621 tctggcataa aatcttcgac gtggaaatcc cacatccggt actggttatc gcccgtgttg
    19681 gcgcgaatat gccacatacc ggttgccgca ttgctatcga gtggccaggt aaagtggtag
    19741 aggccattct ccggctgact aacgacgctc ctgagtacct gcccgtcagg tttaatcacg
    19801 tctaacttga tgggttgatc gggcaatgct ttaccgtctg catcgcgcag taaaccattg
    19861 aggattaccg tttcacctgg gcgataaaga tcgcgtgggc caaacatgaa aaactgtttg
    19921 ctatagcctg gcgcgccagc aatgttaaat tctgctaagt ccagcgccgg aagttttaaa
    19981 tcgagtagcg ttgtctgacc gtctttacgt gccagcagta atgccgcatt tttatcattt
    20041 tccagctgca catgcccctg agcgtcactg gttgcctgag tcagagtctg ccctttctca
    20101 tttaataaag agacttcaat tccttgctgg gccgcgccgt tttccagact ttgggtaaag
    20161 atatccagac gattgtgata acggtgagct gaaacgccga tatcacttaa cgtaaacagc
    20221 gtcgcgggat tactgtaatc gtaacgtcca gcctgattca tcacagccag atacacgccc
    20281 gcctgctgaa gcggtttgat atcgcccagc ggcagcaata atttttcacg ggtgttacgc
    20341 gcaggattga gatcaaaccg tccggtgtag accagatccg ccatctgcag cagtttgtct
    20401 gactgccagt tcgccagcga attgcggtat tcccattggc taatgaatgc tggcagagat
    20461 tctggcttaa cgcggaagaa gttaacatcg acattattaa cgttgagcgc cattaccggc
    20521 agcccttcaa cgactttgcc aggcagcagc gaaccacggc tggcaaaacc gacgctgggt
    20581 tggatgtcgc gggtagttat agttttttcg taatctttac tgaaggttgc gttgttgagt
    20641 gctttgacct ccttgccaat agtaacgatc aaatcacgtt tcggttcgag gtggcgtaaa
    20701 cgtagctctt taagattatc tgacagctcc caggcaccat ccactttgcc gcttttttta
    20761 tcgacgacat gaataacgcg tgagaaatcc tgatc
//
LOCUS       DAJTYD010000056        20754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTYD010000056 DAJTYD010000000
VERSION     DAJTYD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.056"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO585_001884"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO585_001884"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6629496.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO585_001885"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO585_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6629497.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OO585_001886"
     CDS             complement(2058..2672)
                     /locus_tag="OO585_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6629498.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO585_001887"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO585_001887"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6629499.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OO585_001888"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OO585_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6629500.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OO585_001889"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OO585_001889"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6629501.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO585_001890"
     CDS             complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO585_001890"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6629502.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO585_001891"
     CDS             complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO585_001891"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6629503.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10154..10642
                     /gene="rfaH"
                     /locus_tag="OO585_001892"
     CDS             10154..10642
                     /gene="rfaH"
                     /locus_tag="OO585_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6629504.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO585_001893"
     CDS             complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO585_001893"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6629505.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO585_001894"
     CDS             complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO585_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6629506.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO585_001895"
     CDS             complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO585_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6629507.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO585_001896"
     CDS             complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO585_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6629508.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO585_001897"
     CDS             complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO585_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6629509.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO585_001898"
     CDS             complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO585_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6629510.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO585_001899"
     CDS             complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO585_001899"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6629511.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO585_001900"
     CDS             complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO585_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6629512.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO585_001901"
     CDS             complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO585_001901"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6629513.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18806..19618
                     /locus_tag="OO585_001902"
     CDS             18806..19618
                     /locus_tag="OO585_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6629514.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20012..20672
                     /gene="hcp"
                     /locus_tag="OO585_001903"
                     /pseudo
     CDS             20012..20672
                     /gene="hcp"
                     /locus_tag="OO585_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5080 a         5604 c         5210 g         4860 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aatcagctta atcataaaac ggtcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg tgaactcatc agaaacttgc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcatagtttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgagg caaaggcaga aaaatcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacaa ccggagatca gcaggaagat agcaatgata gtgttaatag tcggactgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc aaaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttgcggt tcggccacca
     1861 cagcatagag ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaatgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgactata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagcggtaat ttccccgatg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctctttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt attttcgtgg atcaccacgt tgtcttcgat acgaatgccg ctgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcgatgaag tagatacccg gttcgatggt taacaccatg cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatgg ccgataccgt
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc aatttggcga tgcgctgatg gaactggagg tggtaatcca
     3121 catagctgac gccagctttc atggtagcga tcagcgcaag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttacat gtttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg aaccactaaa
     4861 actgagcaag attgaagcca ccatgagctt cactatcgct aaagggatgg tcgcacaaac
     4921 ggcggggaaa cattatccgg cgcccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcat taaatctgga aaacaaaagc tttgtcccgc tggcgcacac
     5041 caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacacc gaaacaggcc gctgtactgg gcgcaggcat
     5161 catgggtggc ggcattgctt accagtctgc gtggaaaggc gtaccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgaacgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccgggt ttgaccgcgt ggatgttgtg gtagaagcgg ttgtcgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc cggataccgt
     5461 actggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcggaatgc acttttttaa cccggtacac cggatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gcctatgggt ccggcatatc tgctggatgt
     5821 cgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tccgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctagcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcatgt ctggttggcg gcgtggagca tatgggccat gtgccaatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaggtgat
     7021 tcgaccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaaa
     7081 cggtacggta acggcgggca catcttctgc actttccgat ggcgcagctg ccatgctggt
     7141 gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtaacc catcgattat gggttacggc ccggttccgg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggct gttccggtgc
     7441 gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggtttttt
     7741 tatatctcag ataaatgcaa acgcatcgcc aaacagacga tcttcccgcg cattacgctc
     7801 actgcaaaac aggtcacgag caattttcgc catctcaaaa cgtccggcaa tatagatatc
     7861 atgctctgcc agcgtaccat gatcctgcaa gaccgccgtt aacacggtgc cagtacgccc
     7921 acgccagccc gcttccggtt gttcaaccac cggcaccaca cgcaggccag gatgcttcag
     7981 cgaaagcgcc tcaagctcgg agagatcata aagatgttgc tcctcacgcc caccccagta
     8041 aatggtgata tcgcgattgg gattacgcgc caacgccgtc aataaaatcg agcgcgcata
     8101 ggagaacccg gtgccgcccg caatcaaaat catcggacgc tcttcatcat cgcgcagcca
     8161 cgcttctccg tagggaatat cgaccacgat ttgataatct ttgaggatgc ggtccatgac
     8221 tgctttcgcg taaaggttga tttcagaagc gccaatatgc agctctataa accctttttc
     8281 atccggcgtc gaagccattg aaaacggacg tttgtcgcgc tcatccatca ctaccatcaa
     8341 atactgacca gcacgaaaag aaaaggccgc gtctggcacg atgcggacac gatatacggt
     8401 atccgtgata gcttctaccg aggtcacttt acagcttaag gttgtcatgc gctttctctg
     8461 tcggatcgat aaatagggca aaacaaacgc gcatcaggcg cttttaccgt tgttaaaaat
     8521 agccagttca tcccagatgg cgtcaatatg cgcgacaaca tctggatctt ttttgatggg
     8581 acgtccccat tcacgctggg tttcccccgg ccatttattc gtggcatcca gccccatttt
     8641 tgaacccagc ccggagacag gcgaggcaaa atccagataa tcaataggcg tattttctac
     8701 cagaacagta tcccgcgccg ggtccatacg ggtggtaatc gcccaaatca catcgttcca
     8761 gtcgcgtgcg ttgacgtcat catcgcaaac gatcacaaat ttagtgtaca taaactggcg
     8821 taagaacgac cagacgccca tcatgacgcg cttcgcgtgt ccggcgtact gttttttgat
     8881 cgtcactacc gccagacgat aagaacagcc ttccggcggc aggtaaaaat cgacaatttc
     8941 cgggaactgt ttttgcagaa tcggtacaaa cacttcgttc aacgccacgc ccagtaccgc
     9001 gggctcatct ggcggacgcc cggtataggt ggaatggtaa atcgcatctt cacgctgggt
     9061 aatatgcgtc acggtaaata ccgggaaact atcgacttcg ttatagtaac cggtgtggtc
     9121 gccatacggc ccttccggcg cagtttcgcc ttgttcgata tacccttcca gcacaatctc
     9181 cgcactggcg ggcacttcaa ggtcattgga gatacacttc accacttcgg tcttggtacc
     9241 gcgcagcaat ccggcaaacg catactctga aagtgtatcc ggaacggggg tgactgcacc
     9301 gagaatcgtg gcgggatcgg cacccagcgc cacagaaacc gggaaacgtt cgcccggatg
     9361 cgccgcacac cactcctgat aatccagcgc gccgccgcga tgcgacagcc agcgcataat
     9421 cagtttgttt ttaccaatca gctgctggcg ataaatgccc agattctgcc gctctttatg
     9481 cgggccgcgc gtcactgtca gcccccaggt aatcagcggt gcggcatctt ccggccagca
     9541 ggtcataatg ggaatgcgat tgagatcgac gtcatcgcca gagacgattt tttgttggca
     9601 gggcacacca cgcagccgct ttgtcggcat gttcaatact tgcttaaact gcggcagttt
     9661 atcaaacagg tcgcggaaac cttttggcgg ctccggctct ttcagaaacg ccaataattt
     9721 accaacttca cgcagcgccg aaacatcttc ctgtcccatg cccatcgcca cgcgctttgg
     9781 cgtaccgaac aggttgcaca gcaccggcat tgagtagcct ttagggtttt cgaacaacag
     9841 cgcaggccca ccggcacgca aagtgcggtc agcaatttca gtgatttcca gatgcggatc
     9901 caccgggagc gtgatacgtt ttagctcacc ctgctgttca agcagcgtca agaagtcgcg
     9961 taaatcgtta tatttcatgg cgtccattgt agcctcttaa tctacgccca ttatacggcg
    10021 ttcatctttg cgatgctgta aatttgttaa attagcgtga actctgacgg tataacgcaa
    10081 accggggaat ataattaact tagcgtaaag cttttgctat ccttgcgccc cgattaaacg
    10141 gataagagtc attatgcaat cctggtattt actgtactgc aagcgcgggc aacttcaacg
    10201 tgcccaggaa cacctcgaaa gacaggctgt gaattgcctg gcaccgatga tcaccctgga
    10261 aaaaatcgtg cgtggaaaac gtactgcagt cagtgagcca ttgtttccca actacctgtt
    10321 tgtcgaattt gatccagaag tgattcatac cacgactatc aacgcgaccc gcggtgtcag
    10381 ccacttcgtg cgctttggcg cgtcgccagc gatagtccca tcggcggtga ttcatcagct
    10441 atcggtatat aaaccgaaag acattgtcga tccggcaaca ccttatccgg gtgataaggt
    10501 gattattacc gaaggcgcgt tcgaaggctt tcaggccatt ttcaccgaac ccgatggtga
    10561 ggctcgttcc atgctattgc ttaatcttat taataaagag attaagcaca gtgtgaagaa
    10621 caccgagttc cgcaaactct aaaacgcaat cccaaacagt gttttgacat tggcatccgt
    10681 ggtggcagcc agccatgcgg catcttctcc acgccagtgc gcaatacgtt gcaaaatatg
    10741 gtgcagatgg gctggctcgt tgcgccggga tgatggcttt ggcgtgaaat cgcgcgggag
    10801 cagatacggc gcatcagttt cgatcagcaa tttctccgcc ggaatcaacg gcaacaattc
    10861 ccgcagctcc agcccgcgtc gttcatcgca aacccaaccg gtaatgccga tataaattcc
    10921 acgcgccacg cacgcctgca tctcttcgcg tgtgccggta aagcaatgaa gaaccgcacc
    10981 aggcagttta tccagccacg gctccagcaa tgtcataaac cgctcgtggg catcgcgaca
    11041 gtgcataaat accggcatgt ttaattctgc ggcaatgcgt agctgagcaa caaaagcgcg
    11101 ttcctgctct tccggcgtcg aaaagttgcg gttaaagtcg agaccacatt caccaatcgc
    11161 caccacttct ggctgcgcgg ccagctcaat aatcgcttct tcagtcgcag cttgccactg
    11221 gctgctgtcg tgaggatgta cgcccgccgt tgaccaacag gacgaatact gacgcgccag
    11281 cttttgcgcc tgctggcttt cacgcaggtt agtgccggtg atgagtagcc cattaactcc
    11341 cgcgtcaaaa gcgcgcgcta caacatcatc acggtctttc gcaaattgcg aactggtcaa
    11401 attaacgccg atatcaaaca tcctgtactc catatgacaa ccgccctgac gggcggttga
    11461 atttattctt cagttttttc gctttctgct tcagcgtcgt tttcctcttc ccgatttcgc
    11521 cctttaccaa cgtaaaagcg tgagaagaag acaccgattt caaacagaca gtacatcggg
    11581 atcgccaata gcgtttgcga gaagacatcc ggcggcgtca gcaacatccc gacaacgaat
    11641 gcaccaacca gcacatacgg gcgttttttg cgtaagtctt ctggcgaggt aatccccatc
    11701 cagcacagca gcacaattgc caccggcact tcaaaggaga caccaaacgc cataaacagc
    11761 gccataacga agcttaaata gctggcgatg tcggtggata cctgcacccc ttccggcgcg
    11821 gtattggcaa ggaagccaaa tgccagcgga aagaccacaa agtaggcgaa tgccatgccg
    11881 atataaaaca gcagagagct ggaaaccagc agcggcacca ccaggcgacg ttcatgctta
    11941 tacagcgctg gggcgataaa tgcccacacc tgatagagaa tcaccggcgc tgacagaatc
    12001 agcgacacca taaaggtcag cttgatcggc gtaaagaacg gcgaggccac gtcggtggcg
    12061 atcatcgttg aaccttgcgg caactgcttg atcaatggcg cggataccag gtgatagatg
    12121 tcattggcga aatagaccag acacaggaat atcacgatca ccgcgataat gcagttcagc
    12181 agacgcttac gcagctcaat tagatgcgtg ataagcggtt gagtatcttc tacagacatg
    12241 tttacggttt atcactcgac gaaggggaag gtgcagcggt tttcggttca gcgtccgcag
    12301 caggttttac caccggctct ggcgtggttt ctggcttctg ttccggcgaa ctggcctgcg
    12361 tttgtgcagc ggcaggcgtt acgccctcat gcgcagcttc attatctttc accaccgggt
    12421 tatggatggt gtgcgcttca tcgctcgcct tttctggatc gtttgcaacg taggaacgct
    12481 tcatcgactc cgcggcctgg cgtagttcat ccatcgacgc tttcagttcg ggcgtcaggt
    12541 tagtgaggct cgccttttca acctttttca gactgtcctg aaactcctgg agttttaact
    12601 cctggatcag ttcgttctgc accgttgtcg ccagtgaacg caacgcgcga atccagcccg
    12661 ctaccgtttt taccgccaca ggcagtcgtt gcggccccag aacgacgagg ccgatgatga
    12721 acaccaatag cagttcgcta aaaccgatat caaacacgga ttacacctgc tctttatcgt
    12781 ggcgcttcgc gtcttctgtt ttagcctgtt cctgattcgt atccgcctgc ttatcggcga
    12841 tagttttcgc agtaaaatca gcatcctgac tggttttatc ctgctttggt tcatcatcgc
    12901 tcattgcttt tttaaagcct ttgatcgacg caccaagatc ggaaccgatg gagccgagct
    12961 ttttggtgcc aaaaagcagt acaacgatga cggcaataat caataactgc caaatactga
    13021 taccacccat acatgttcct ctgtggtaga tgatgattaa acaaagccgc attatacgtt
    13081 acacggcccg ccttgagcga tgaaaaaatc agcgtgtttt gcgccaaccg acaaaccagg
    13141 cgatcagacc acctgccatt aaccagccgg gcatcagccc ccactcaggt cggctgacca
    13201 acaagaatgt gccacttaat actaacgtag cgccaattcc gagaaaataa cgcgattgtc
    13261 cctgacgtac atgatttgac tgaagctcgc gggcaatctt atcaacactg tgctgtaaat
    13321 acttgccctg gcgcaaactg tcgtaaacca attcaggcag ttctggcatt ttttcgaccc
    13381 agaacggcgc tttttcttta aatgctctca ccagcgcagg aataccgacc tgatctttaa
    13441 tccacgactc caggaaaggc ttcgctgttt tccataaatc gagctgcgga taaagctggc
    13501 gtcctacccc ttcgacatag agcagggttt tctggagtaa caccagttgc ggctgcactt
    13561 ccatattgaa gcgacgcgcc gtattaaaca gatttaacag tacatgtcca aacgaaattt
    13621 cggccagcgg tttctcaaag ataggttcac agaccgtacg aatggcaaat tcgaactctt
    13681 caacgttggt atctggtggc acccagcctg aatcgacgtg tagctctgcc actttgcgat
    13741 agtcgcgatt aaagaaggcg ataaagtttt ctgccagata gcgtttatct tctttgttta
    13801 gcgagccaac aatcccgcaa tcaatgccga tatatttcgg gttttccggg tgttcatagc
    13861 ttacgaagat gttgccaggg tgcatatcgg catggaaaaa gctgtcgcga aagacctgag
    13921 tgaagaacac ctgcacgccg cgttccgcca gcaatttcat gttagtgccg tttttctcca
    13981 gcgccgcaac atcagacacc ggaatgccgt aaatgcgctc catcaccatc atcccttcac
    14041 tacaatagtc agggtaaact tccgggatgt agagcatcgg gctgtcttca aaattgcgcc
    14101 gaagctgaat ggcgttggca gattcccgca gcaaattcag ttcatcaatc aaggtctttt
    14161 cgtactcgcg caccacttcg gttgggcgca gacggcgacc atccggcagc aaacgcggca
    14221 cccagcgagc cagacggtag ataagtttca gatccgcttt aatgaccggc aaaatatccg
    14281 ggcggatgac tttaatcacc acctctttac cattcgattt caatcgcgcg gtatgaacct
    14341 gggcgataga agcagaagcc agcggcttga tttcaaaatc gtcaaaccac gcttctaccg
    14401 gcaagccgcc cattgcagct tcaatctgct gcttcgccag cttgccatca aacggagcta
    14461 ctttgtcctg caataacgcc agctgatcgg caatatgcgg tggaaaaaga tcgcggcggg
    14521 ttgataacat ttgcccgaac ttgatccaaa ccggccccag ttcttgcagg gccagtcgta
    14581 gtcgctcacc taaaagtttg tctttatgcc gatttggcat ccagaataat gagtatcgcc
    14641 atagccgtag cggcagggtg atacgcattt tggggatcag ttcatcaagt ccgtagctta
    14701 aaaaagtgcg aatgatgaaa tataggcgcc gtacttcacc tggcgtcatt tagcctccag
    14761 tttttccagc cgtttggtca gggcatcaac agcacgctcg acggcagccg tctcttccgc
    14821 aaaccaggcc acttcaagcg gaccgggtgc catacgccac tcttcagtaa tggcttccgc
    14881 cacataacgt tgctggcgct taatgccgtg atgcaggaac tttgcgcctc cgcgcatggc
    14941 tttgctgatt ccttcagcgg cgatatcacc ggtataaggg gccagcagtt ccgcagggtc
    15001 gaactctgcc agatctgcca gcgcaacgaa gttttgcacc acctgaatat cgccctgcac
    15061 ttccagctca ccactgcgaa tcagtgcggt aagctgctgg cgatcgcgaa gtttcggcaa
    15121 cacactggcg taggcgataa cggtgcagtc agcatcgcct gcccattcgc ccagtacatc
    15181 aacctggcgt tcgctgaaca ccagaatcaa tgacgtcgaa aagcctttta cctccacgcg
    15241 caatacttta cccagcagac gcgagcgggc tgttttcagc gcgggtgagc gatacaggaa
    15301 ggtgttgagc agactttcaa ttcctgccgt cactaaaggt ttaaaaggca tttccggtct
    15361 cctgtcagaa cttataacca cgatgcagcg ccacaacccc tgccgtcaga ttgtagtagt
    15421 cgacactttc gaatccggca tcctgcatca tggctttcag ggtatcctga tcgggatgca
    15481 tacggatgga ttctgccaga taacggtagc tgtcggcgtc gttcgcgacc agtgagccaa
    15541 tacgcggcag cacatggaag gagtatgcat cataggcttt gctcagcggc tcgataattg
    15601 gcttcgagaa ctcaagcacc agcaggcggc cgccgggttt cagcacgcga tacattgaac
    15661 gcagtgcttt atctttgtcg gtgacgttac gcagaccaaa cgaaatggtg atgcaatcaa
    15721 aggtgttatc cgggaacggc agcgcctcag cgttcgcctg aacatactca acgttgccaa
    15781 tcacaccgat attacgcagc ttctcgcgtc ccattttgag catggattca ttgatatcag
    15841 caaggaccac tttgccagtt tctccgacca ggcgggagaa tttcgctgtc aggtcgccgg
    15901 tgccaccagc cagatccagc acggtctgcc cacggcgtac gccgctgcaa tcaatcgtga
    15961 atcgcttcca caaacgatga ataccaaatg acatcaaatc attcatgaca tcgtattttg
    16021 atgccacgga atggaaaacg tgggcgacca tatccgcttt ttgttccttc gcgacggtct
    16081 gaaaaccaaa gtgcgtcgtt tcttgtgact tatccaccat ctcaatgcct gctcatcaaa
    16141 aaattgttcc agaagtgtaa cagattgggc gtcgatgccc tagatttcta cccggcttaa
    16201 ctacccctaa tgggctagcg cgactgctga ttatattcat catcgcgttg ataagcttca
    16261 tcattcggct gctccggaac cgaccgaagt cgatactctt catcctggct caccgcctgt
    16321 tcagccaaat ccggattaat ctcgcgttta atttctactc ctaaaccgcg aaacgcttct
    16381 gcctgcgcca gcacatttcc gcgacctgaa gagagttttt tcattgcctg gcggtagtta
    16441 tcctgcgctt tgtcgagact ttgaccaatc gcggacatgt catcgatgaa caaacgcatc
    16501 ttgtcgtaca gcttgctggc acgatcggcg atttgctggg cgttgcggct ttgatgctca
    16561 taacgccaca ggttggcaat agtacgcaac gccaccagca gcgtagtcgg gctaaccagc
    16621 atgatattgt ttttcaacgc ttcggtgatc agctccggct ggcggtcaag cgccagtaaa
    16681 aaagcgggtt caacgggaat aaacatcagc acgtaatcca gagttcgcag ccccggcagc
    16741 tgttgataat ctttgcgtcc cagcaaacgg atatggttac gcaccgacgc gatatgttcc
    16801 tgtagcgcgc tttcgcgggt gtagtcgtct tcggcgttaa aatagcgttc ataggcgacc
    16861 agcgtcattt tggcgtcgat caccacatct tttccctgcg gcagccgcac gatgacatcc
    16921 ggctgcatcc gcgagcgggc gtcattttcg atgctgacct gggtttcata ttcataccct
    16981 tcacgcagac cggaagcctc cagcacccgc gtcaatacta cctcgcccca gttgccctgg
    17041 gttttattgt cgcctttcag cgcgcgcgtc aggttgatcg cttcctgggc catttgcgcg
    17101 ttgagttgct ggagattgcg aatttcgtgg gtcagggtat ggcgttcttg tgcttcttta
    17161 ccgaagctgt cctgaacctg acggcggaaa ccgtccagtt gttcacgtag cggcgacaac
    17221 aggctgttca gactctgacg gttttgctca tcaacccggc gattgctgtg ctcaaaaata
    17281 cggttggcga ggttttcaaa ctgctcactg aggcgctgct cgctgttaat catctggcga
    17341 attttatcgt cagcatgttg ctgtgcggct tccatccgcg tggttacttc acgcagatcg
    17401 gcctccagag aggtgttaat actttgcagg ctgcgcactt cgttattgag taactcgcac
    17461 tctgcacgcc agtgctcgct ttgggtaatt tgttgttttg ccgcgcttaa ctccgcgacc
    17521 atctcttcac gttcagctaa ttgctcggct ttttgctgcg catgttgata actggcaaac
    17581 agccagccaa ttgccacacc caccaacgca ataactgcgt aaaccatgat tgagaaatcc
    17641 acaacgcctc ctgacgcaac aacttacccg cacaaaatag aattgtgctg tacaaacgtc
    17701 cagttggaaa ggcatttcct gcgaggcgct acgcaaaaat acaaaaggcc gaacgcgtcg
    17761 gccttcagac aggagaagag aattacagca gacgacgcgc cgcttccacc acgattttca
    17821 ccgcttggct ttcagtttgt ttcatcgtct cagcattcgg gatctcttgc tgggtgcggt
    17881 taacgataac acccgctacc ataccggcac gcaggccctg acttgcacac atggtcagca
    17941 gggttgcaga ttccatttca tagttcatta cgcccatcgc ctgccactct tccatagaac
    18001 ctttaaagtg acgaactacg cgaccagagt aagtatcgta acgttcctgg cctgggtaga
    18061 aggtatcaga agaagctgtc acgccaacgt gagttgtcgc gccaatggat ttcgcagctt
    18121 caaccagcgc agtcgtacat tcgaaatcag cgacagccgg gaattccagc ggtgcgaagt
    18181 gcaggctcgc gccatccaga cggacagacg ccgtggtaac caggacatca cccacattaa
    18241 tatgcggctg aatagcgccc gttgtaccga tacgcaggaa ggtgcgaatg cccagctgtg
    18301 ccagctcttc aacagcaata gaggtagacg ggccgccgat accggtagag cagacgataa
    18361 caggtttacc atccagctct gcacgccagg tggtgaattc gcggtgagat gccagcttaa
    18421 ccggcttatc catcagcgcg gcgatctttt ccacacgatc cgggtcgcca gggacgatgg
    18481 caagcgtagc cccttgtaaa tcgtttttag tgaggccgag atgaaaaaca tcagacttgg
    18541 acatatacaa ctcctctgtg aatcggttta gtcagaagaa gcaaaaagac actttaccga
    18601 agggtttaac attttttcgt gatactcatc accatgacgc aaatgcgttg cataaattta
    18661 cccaccaaaa gtgatgcaaa tcacataaat cactcttcat tctgcaggac tgcataaaag
    18721 atgagccgat tcaggcaatg tgatttgttg catgctgact atagttaaca ttgcgcaaca
    18781 tttttataag ggtacggaga ataccatggc aacaacacaa caatctggat ttgcacctgc
    18841 tgcatcgcct ctcgcttcga ccatcgttca gaccccggac gacgcgattg tggcgggctt
    18901 cacctctatc ccttcacaag gggataacat gcctgcttac catgccagac caaagcaaag
    18961 cgatggccca ctgccagtgg tcattgtagt gcaggaaatt tttggcatgc atgaacatat
    19021 ccgcgacatt tgtcgccgtc tggcgctgga ggggtatctg gctatcgcac ctgaacttta
    19081 cttccgcgaa ggcgatccga atgattttgc cgatatcccc acgttgctta gcggtctggt
    19141 agcaaaagtg cctgactcgc aggtgctggc cgatctcgat catgtcgcca gttgggcttc
    19201 ccgcaacggc ggcgatgttc atcgtttaat gatcaccgga ttctgctggg gtggacgtat
    19261 cacctggctg tatgccgcgc ataatccaca gctaaaagcc gcagtggcgt ggtacggcaa
    19321 actgacgggc gacaagtcgc taaattcacc gaaacaacct gttgatatcg caaccgatct
    19381 taacgcgccg gttctcggct tatatggtgg tcaggataac agcattccgc aggagagcgt
    19441 tgaaaccatg cgccaggcgc tgcgggctgc taatgcgaaa gcagagatta tcgtgtaccc
    19501 ggacgccggg catgcattca acgctgatta tcgcccgagc tatcatgccg aatctgcgaa
    19561 agatggctgg cagcgtatgc tggaatggtt taagcagtat ggtgggaagc ggagttagtt
    19621 tcctcttagt ctatatttga gatggtgagt gaacgagtgc gttttgctgc catctctgtc
    19681 ccccaaaaaa taatcagtat gaattgattg gagattacga ctccgctatt tgaccttaag
    19741 aaagtcggca agtcatagtc ataaatgagt ctcttcaatg gaagacacct tagagattta
    19801 ccatctactc ccattcacaa acaaacatta attattatca acacattcac attaattata
    19861 aatcatctca gaccttcctg ataaaaagat attaaagtga aacattttgt ttttcacatt
    19921 ttagttcatc acttttaggc gtaaataaag gtaaaaaaaa caacaatact atttctcttt
    19981 cacaaaagaa ttaacaaaag gttttttaag gatgagtgat attatttact tgaccataac
    20041 cggagaacaa caaggatgta tttcctctct ttgtggaacg agcgcatcaa taggtaatcg
    20101 ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctctctaata gtattacgaa
    20161 tgcaggcaaa gggtcacaat tgcatggcct tagtttctgt aaattcttat taacgctatt
    20221 aacaataacg agcagttgtt catggcgttt gacttctatc ggatcaatag atttggtcga
    20281 tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg
    20341 tttactgaga acaatctgga tacagaagcc atcactgtca gttatgaata tatactctcc
    20401 aggcatctga ttgctaatac tgaatttagt tatttggcat ttcccgataa ctacaaccgt
    20461 ctgtttatac cacggccaaa aactaaagct ggtaatggtt caaaacactt aatagtaaag
    20521 cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa ggattcaggg
    20581 aaacagcaga caagttgggg ggagatgcac ctgctggtta cgatcaattt atggataaca
    20641 aagggttact catcgctggt gcttcaatag ttgctggagc agttctgggg agaatgagac
    20701 tccctgattt ggaatcactt gagcatttcg gagccagagg agcagtatca ggaa
//
LOCUS       DAJTYD010000057        20697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTYD010000057 DAJTYD010000000
VERSION     DAJTYD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.057"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..222)
                     /gene="hyfA"
                     /locus_tag="OO585_001904"
     CDS             complement(<1..222)
                     /gene="hyfA"
                     /locus_tag="OO585_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6629515.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDA"
     gene            complement(475..945)
                     /gene="bcp"
                     /locus_tag="OO585_001905"
     CDS             complement(475..945)
                     /gene="bcp"
                     /locus_tag="OO585_001905"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6629516.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(945..1517)
                     /locus_tag="OO585_001906"
     CDS             complement(945..1517)
                     /locus_tag="OO585_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6629517.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1663..2541
                     /gene="dapA"
                     /locus_tag="OO585_001907"
     CDS             1663..2541
                     /gene="dapA"
                     /locus_tag="OO585_001907"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6629518.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2558..3592
                     /gene="bamC"
                     /locus_tag="OO585_001908"
     CDS             2558..3592
                     /gene="bamC"
                     /locus_tag="OO585_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6629519.1"
                     /translation="MAYSVQKSRQAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3805..4518
                     /gene="purC"
                     /locus_tag="OO585_001909"
     CDS             3805..4518
                     /gene="purC"
                     /locus_tag="OO585_001909"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6629520.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4686..5549
                     /locus_tag="OO585_001910"
     CDS             4686..5549
                     /locus_tag="OO585_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6629521.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5564..7579
                     /gene="tmcA"
                     /locus_tag="OO585_001911"
     CDS             5564..7579
                     /gene="tmcA"
                     /locus_tag="OO585_001911"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6629522.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7653..8351
                     /gene="ypfH"
                     /locus_tag="OO585_001912"
     CDS             7653..8351
                     /gene="ypfH"
                     /locus_tag="OO585_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6629523.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8461..8661)
                     /locus_tag="OO585_001913"
     CDS             complement(8461..8661)
                     /locus_tag="OO585_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6629524.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8689..9816)
                     /gene="dapE"
                     /locus_tag="OO585_001914"
     CDS             complement(8689..9816)
                     /gene="dapE"
                     /locus_tag="OO585_001914"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6629525.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9820..10176)
                     /locus_tag="OO585_001915"
     CDS             complement(9820..10176)
                     /locus_tag="OO585_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6629526.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10557..10616
                     /gene="ypfM"
                     /locus_tag="OO585_001916"
     CDS             10557..10616
                     /gene="ypfM"
                     /locus_tag="OO585_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6629527.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10715..13827)
                     /gene="acrD"
                     /locus_tag="OO585_001917"
                     /pseudo
     CDS             complement(10715..13827)
                     /gene="acrD"
                     /locus_tag="OO585_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(13990..15690)
                     /gene="narQ"
                     /locus_tag="OO585_001918"
     CDS             complement(13990..15690)
                     /gene="narQ"
                     /locus_tag="OO585_001918"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6629528.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15896..17875
                     /gene="aegA"
                     /locus_tag="OO585_001919"
                     /pseudo
     CDS             15896..17875
                     /gene="aegA"
                     /locus_tag="OO585_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            17943..18518
                     /gene="nudK"
                     /locus_tag="OO585_001920"
     CDS             17943..18518
                     /gene="nudK"
                     /locus_tag="OO585_001920"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6629529.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18644..19687
                     /locus_tag="OO585_001921"
     CDS             18644..19687
                     /locus_tag="OO585_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6629530.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19797..20697)
                     /locus_tag="OO585_001922"
                     /pseudo
     CDS             complement(19797..20697)
                     /locus_tag="OO585_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         5001 a         5294 c         5540 g         4862 t
ORIGIN      
        1 cgcatcatcc ctctgggaga tggcattgac cgggcagacc tgcaggcaag gggcttcctc
       61 acagtgatga cacacgacag gggcagtgat tgttgacgtc ttcgccaggg ccaggcgcgg
      121 gtgttgctgt aaaccttgcg ttttatgcac gtccgaacag gcagcgagac aggtattaca
      181 tcctgtacac cacagtggtt cggccaccac aaagcggttc atgtcggctc cttgaaaatg
      241 atatgaaaca ttgcatttca tattgcataa aggatgccag tctgattaat ttaatatatt
      301 caataaatta tgtattaaca gggttctgaa cggtacaaaa atgatggtca ttaacgttca
      361 gcaaaaaatg gattcgacac ccgtgtcgca ggggaaagcg agaatgtgat cttgatcacg
      421 agaagtgagg tgtgctgacc gcaagcgcag ccagcacgga atggagcaaa gtaatcaggc
      481 gtgttctttc agccagttca gcacaacgtc gtggtgattg ctggttttaa aatcgtcaaa
      541 gacatgttcg attttgccat cagcgtcaat caggaagctg atgcgatgaa taccatcgta
      601 ggttttgccc atgaaggact tttcacccca gacgccgaat tgttcgcaca cctggtggtc
      661 ctcatcagac aggagcgtaa agttaagcag ctctttttcc gcaaaacggg agagtttttc
      721 gggtttatcg gtgctgatac ccagcacatc aacgcccgct tttttcaact catccatgtt
      781 atcgcgtaag ccgcaggcct gtacggtaca gccgggggtc atggctttcg ggtagaaata
      841 aaccagaaca cgctgtccct ggaagtcggt caaattaact tgttctccgt cttgatccgg
      901 caagctaaat ttcggtgcga tatcaccggc tttcagtgga ttcattactt aactccatcc
      961 tgttcatcat gttgggaata attgacgacg ttaatactgc cttgtgcatt gagttctgta
     1021 catagggctt tgaacgcttg ctcaatattt gctgcgtccg cagatgcggg gctgtgggcg
     1081 gtgatctgaa tatgcaactg cgcagcccgt tcattttcag caggttgcgt gcgcgacacc
     1141 agctccgcaa tgttcatatg atgcgcgtcg aaaagtgctg tgaagcgttc aattaaatgc
     1201 ggggagtctg caacatcgac ctgaacccag acagatgctg gcattggcgg acgcggacgc
     1261 gccgtcgtgc gcttcatcac gattaaaaga tccagttcgg cacctttcaa cggtaacgtt
     1321 gattcaatca gagtaatggc attccatgaa ccggaaagca gcatgataaa cgtgaactct
     1381 tctcccagca tcgccaggcg actgtcttca atattacagc cgcaactact gacatgacgg
     1441 gtgatggtgt tcacaattcc agggcgatcg gcacccaacg cagtgatcac cagataatgt
     1501 tgcgatgaca gtgtcaaact ggttattcct ttaaggggtg agttgttctt aaggaaagca
     1561 taaaaaaaac atgcatacaa caatcagaac ggttctgtct gcttgctttt aatgccatac
     1621 caaacgtacc attgagacac ttgtttgcac agaggatggc ccatgttcac gggaagtatt
     1681 gtcgcgattg ttactccgat ggatgaaaaa ggtaatgtct gtcgggctag cttgaaaaaa
     1741 ctgattgatt atcatgtcgc cagcggtact tcggcgatcg tttctgttgg caccactggc
     1801 gagtccgcta ccttaaatca tgacgaacat gctgatgtgg tgatgatgac gctggagctg
     1861 gctgacgggc gcattccggt gattgccggg accggtgcta acgctactgc ggaagccatt
     1921 agcctgacgc agcgcttcaa tgacagcggt atcgtcggct gcctgacggt aaccccttac
     1981 tacaatcgtc cgtcgcaaga aggtttgtat cagcatttca aagccatcgc tgaacatact
     2041 gacctgccgc aaattctgta taatgtgccg tcccgtactg gctgcgatct gctcccggaa
     2101 acggtgggcc gtctggcgaa agtaaaaaat attatcggaa tcaaagaggc aacagggaac
     2161 ttaacgcgtg taaaccagat caaagagctg gtttcagatg attttgttct gctgagcggc
     2221 gatgatgcga gcgcgctgga cttcatgcaa ttaggcggtc atggggttat ttccgttacg
     2281 gctaacgtcg cagcgcgtga tatggcccag atgtgcaaac tggcagcaga agggcatttt
     2341 gccgaggcac gcgttattaa tcagcgtctg atgccattac acaacaaact atttgtcgaa
     2401 cccaatccaa tcccggtgaa atgggcatgt aaggaactgg gtcttgtggc gaccgatacg
     2461 ctgcgcctgc caatgacacc aatcaccgac agtggtcgtg agacggtcag agcggcgctt
     2521 aagcatgccg gtttgctgta aagtttaggg agatttgatg gcttactctg ttcaaaagtc
     2581 gcgccaggca aaggttgcgg gtgtttcgct tattttatta ctcgctgcct gtagttctga
     2641 ctcacgctat aagcgtcagg tcagtggtga tgaagcctac ctggaagcgg caccgcttgc
     2701 ggagcttcat gccccggctg gaatgatttt gccggtgacc tccggtgatt atgcaatccc
     2761 ggtgaccaac ggtagtggtg ctgtcggtaa ggcgctggac attcgtccac cagcccagcc
     2821 gctggcactg gtttctggcg tgcgtaccca gttcacgggc gataccgctt cattgctggt
     2881 ggaaaatggt cgtggcaata ctctgtggcc gcaggtggtt agcgtgctgc aggcgaaaaa
     2941 ctacaccatc acccaacgtg atgatgctgg tcagacactg accatcgatt gggtacaatg
     3001 gaaccgtctg gacgaagacg agcagtatcg tggtcgttat caaatctctg ttaagccgca
     3061 gggttatcag caggcggtta cggttaaact gctgaacctg gaacaggcgg gcaaaccggt
     3121 tgcagacgcg gcttccatgc agcgttacag cacggagatg atgaacgtta tttccgccgg
     3181 tctggataaa tctgcctctg acgccgcgaa cgctgcgcaa aatcgtgcct ccaccactat
     3241 ggacgtacaa agtgcagctg atgacaccgg tttaccgatg ctggtcgtac gcgggccgtt
     3301 caatgtggtt tggcaacgtc tgccagcggc gctggaaaaa gtgggcatga aagtgaccga
     3361 cagcacccgt tcgcagggta acatggccgt aacttataag ccgctgtctg acagcgactg
     3421 gcaggaactg ggcgcgagcg atccaggcct ggcatccggt gactataaac tgcaggttgg
     3481 cgatttagat aaccgcagca gcctacagtt catcgatccg aaaggtcata ctctgactca
     3541 gagtcagaac gacgcgctgg tagctgtctt ccaggctgcg tttagcaagt aaaaatacag
     3601 ggctggagtc atccggccct tttttctgat atgatacgca aacgtgtgcg tcggcagaaa
     3661 aatgcaattt tagcggtaat tcgcacgaaa tttgtttgtc ggacgtaggt cggataaggc
     3721 gttcttgccg catccgacaa aacatccggc acaccagaca gcaaaagatt ttaaaacgtt
     3781 aattcacacc caggagtgat aaagatgcaa aagcaagctg agttgtatcg tggtaaagcg
     3841 aaaaccgtat acagcacgga aaacccggac ctgttggtgc tcgaattccg caatgatacg
     3901 tcagcagggg atggcgcgcg cattgagcag tttgatcgca aaggtatggt gaacaacaag
     3961 ttcaactact tcattatgag caaactggct gaagcgggta tcccgactca aatggagcgt
     4021 ctgctctccg ataccgaatg tctggtgaaa aagctggata tggtgccggt tgagtgtgtc
     4081 gtgcgtaacc gtgctgctgg ctctctggtg aaacgtcttg gaatcgaaga aggtattgag
     4141 ctgaacccgc cgctgttcga tctgttcctg aaaaacgatg ccatgcacga tccgatggtc
     4201 aacgaatctt actgcgaaac ctttggctgg gtgagcaaag agaacctggc gcgtatgaaa
     4261 gagctgacct acaaagcgaa cgacgtgctg aaaaaacggt tcgatgatgc tggtctgatt
     4321 ctggtcgact tcaagctgga gtttggtctg tacaaaggtg aagtggtact gggcgatgag
     4381 ttctccccgg acggcagccg cctgtgggac aaagaaacac tggagaaaat ggacaaagac
     4441 cgtttccgcc agagcctcgg tggcctgatc gaagcctatg aagccgtcgc ccgccgcctg
     4501 ggtgtacagc tggactgatt tttctgttca tcatcttgcc gtgcagctgg cacggcaaga
     4561 caaccgctct cggaatatat gcattgaacg ctcattcctc cgccatttca tcccgctttc
     4621 ctgtggtaat cttcctctga accacctacg atcatttcta taatgaatat attgttgagg
     4681 tatctatgcg ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct
     4741 ccggtggtcc gtctatgggc ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggctga
     4801 ttttactgat agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga
     4861 ctggacagcc ggtttcccaa caacaatcaa cgcggtccat tagccctaat gaagacgaag
     4921 ctgcaaaatt cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg
     4981 agaagatggg taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca
     5041 ccggctgcgg ggcgggccag tccattatgg ggccgttcta ttgcccggcg gatggcacag
     5101 tttatatcga tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt
     5161 ttgcccaggg gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca
     5221 tcgagccgaa agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat
     5281 ctgtgcgtat ggaactccag gccgactgtt ttgccggtgt ctgggggcat aacatgcagc
     5341 agcaaggcgt tctggaaacc ggcgatctgg aagaggcgct gaatgcggcg caggccatcg
     5401 gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt ttcacacatg
     5461 gcacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac
     5521 aatgcaatac ttttggtaaa agcatttaat ttccggggca gggatggctg aactgactgc
     5581 gctttacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag
     5641 cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacttggcga
     5701 ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct cggcgctaca
     5761 aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc
     5821 cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact
     5881 ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg
     5941 ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga
     6001 taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg
     6061 tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca
     6121 gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc
     6181 ggcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc
     6241 aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcgcc
     6301 ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg aagccgcagc
     6361 catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaactac
     6421 tacggtgcag ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt
     6481 tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg gatgcccgct
     6541 ggaaaaaatg gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca
     6601 aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc
     6661 gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg
     6721 ccggatgatg gatgcaccag ggcaatattt tttacaggcg gctggcgaaa acgagattgc
     6781 cggagcgctg tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg
     6841 ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag
     6901 caatccactg gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc
     6961 tcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat atacgcaaga
     7021 cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaacg
     7081 ctgcggtttt gtgctggtac gaatgggtaa tcatcgtgaa gccagcagtg gttgctatac
     7141 ggcgatggcg ctgttaccga tgagtgatgc gggtaaacag ctggctgaac gtgagcatta
     7201 ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacac ttcctgttga
     7261 tccactaaac gatatcgtgc tttctgacga cgactggctt gaactggccg gttttgcttt
     7321 tgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac aaaccagtga
     7381 actggcatta ccggcgctgc gtgggcgttt acagaaaaat gccagtgatg cgcagttatg
     7441 taccacactt aaacttgcag gccgcaagat gttactggtc cgtcagcggg aagaggccgc
     7501 gcatgcgctg ttcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca
     7561 atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga gccagcggcg
     7621 taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa
     7681 gcccggataa acctgtgcaa cagttgttac tgcttttcct cggtgtcggg gataactcgg
     7741 tggcaatggg ggaaataggc agttggtttg caccgctgtt tcctgatgcg ctggtggtga
     7801 gtgtcggtgg cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcggtgcagg
     7861 gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg tttattgaaa
     7921 cggtacgcta ctggcagaaa cagagtggag taggggctaa cgccacggcg ttaatcggtt
     7981 tctctcaagg cgcgattatg gcgctggaga gtattaaagc cgaaccgggc cttgcttcac
     8041 gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aaccgcgtct acagcaacga
     8101 caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat gcggttgcgg
     8161 cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag
     8221 gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc
     8281 cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg
     8341 aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg catcaggcat
     8401 cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg agcactcagc
     8461 ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa
     8521 atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt cgacgcgctt
     8581 gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca gaatccacca
     8641 atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc aggcgacgag
     8701 ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag cgttcacaca
     8761 ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct gcgcccccat
     8821 gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg gtttaatttc
     8881 attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa
     8941 tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt caagcagggc
     9001 aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga agttaaactg
     9061 cacaaacagt tcacccggaa taacattgtt actgcccgtt cccgcctgaa tattagcaat
     9121 ctgcatactg gtcgccggga aaaattcatt gccctgatcc cactcaatag ccactaattc
     9181 attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgcgggt aggccacatg
     9241 cccctgaacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac catttttcac
     9301 cacatcacca accacttcga tactcgacgg ttcgccgacc aggcagtaat cgagacgctc
     9361 attacgtgcc atcaacgctt cgacgacttt taccgtaccg ttgtgggcac tggcttcttc
     9421 atcagaggtg atcagaaatg ccagtcgccc cgtatggttg ggatgttgtg cgacaaagcg
     9481 ttctgccgct accaccatcg ccgccagcga gcctttcata tctgccgcac cgcgcccgaa
     9541 taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat cggcgtcgcc
     9601 aggcggcacc acgtcggtat gcccggcaaa ggctaacgtt tcaccttgcc cacgccatgc
     9661 ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa
     9721 acgttcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat
     9781 aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat ggaaaaattg
     9841 ctgataactg gaatcactga aacccagcag cataggctta ccaggcgcgc agagcaatgg
     9901 acgtttgata attgaaggca tttcagtcat taatgccgcc gcagaggccg catcggtgat
     9961 tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt tcagtaacgc
    10021 ttcccagcct aattcgttga taaaaccgtt caataattcg ctgtccagcc catcgacgcg
    10081 gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag cctttttaat
    10141 ggtgtcacaa tttttgatgc cgtaaagtgt aaccataatg aatccttttt cgatctatga
    10201 gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc aggaaatata
    10261 acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt ttaacgtaaa
    10321 cattaagaat attcatcttg ttatctgtct gtattttaac cagaattaaa tcagtattag
    10381 atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaattatc accgcaggac
    10441 catagtattt acataaggtt tgtcccccgg aggtagtcat cgcagcaatc gagtaaatta
    10501 gcttcaccac ggtatattgt ttccgtagaa tacccataat tataagagag gttgttatga
    10561 ttgaacgtga actggggaac tggaaagact ttatcgaagt tatgcttcgt aagtaattcc
    10621 tgagacaaag tacaaacagc aagaacccgc tgagcaggtt cttaatcgga aagttgaaaa
    10681 aggcgacaca ttagcatgtc gcctttttta ttgcttattc cgggcgcggc ttcagcggga
    10741 agcggcggcg caccagtaca aagaacagcg gcacgaagta aatagccaga atagtggccg
    10801 aaatcatccc gcccattacg ccagtaccca ccgcatgctg accaccggaa ccggcacccg
    10861 tgctggttgc cattggcaat acgccgaaga taaatgccag cgaggtcatc agaatcgggc
    10921 gtaaacgctg acggcaggcg tggagcgtcg cttcaaacag gtcgtggcct ttttggttca
    10981 tctcgttagc aaactcgacg atcaggatgg cgtttttcgc cgataaacca atgaccgtta
    11041 acaggcccac ctggaagtaa acgtcgtttt ccagcccgcg catccaggtt gccagcagcg
    11101 cgccgataac ccccagcggc acgaccagca ttaccgagaa cggcaccgac cagctttcat
    11161 acaacgccgc cagacacagg aataccacca gcaaggaaat ggcgtacagc gccggagcct
    11221 gcgcgccgga aagccgctcc tgatacgaca tcgccgtcca ctccagacca aagccgtttg
    11281 gcaactgctt cactaacgat tccattatat ccatcgccgt accggtactg acccccggtg
    11341 cggcttcccc aacaatctca accgcagaat aaccgttata gcgttccaga cgcggcgagc
    11401 cagtctccca gcgtgaggtc gcgaaagcag agaagggcac catgccgcca tctttatttc
    11461 ggacatacca gagattaatg tcatctggca gcatgcgata cggcgcagct gcctgcacat
    11521 agactttctt cacacgaccg cgatccataa agtcattcac atagctcgaa ccccaggcgg
    11581 tttgcagtgt gtcgttaata tcgtcgatag caacgcccag cgcctgagct ttacgctggt
    11641 cgatatcaat ctgcaactgc ggactgtcat cgaggccgtt atggcgcaca cgggttagct
    11701 ccgggttttc cgccgccagc gccagcaact ggttacgtgc tgccatcagc gcatcgtgac
    11761 ctgctccagc gtggtcctgc aactccatat caaaacccgc agaactacca agaccgctaa
    11821 ttgctggcgg gctgctggcg ataacgcgag cttctttaat cttgttaaac gcctttgttg
    11881 cacgctcgat aatggcaaac gaggtgccgg tcttactgtc gcgttcgctc cagtctttca
    11941 ggcggataaa cattcgcgcc acgttttgcc cgttaccccc agggccagaa ccaacggtgg
    12001 caaacaccga catgatgttg tctttttcat gggtgaagta atatttctcg atttgctcaa
    12061 cgactttcag ggtctgttgt tgggttgaac cgctgggcaa ttgtaccgag gtagtaaaca
    12121 tgccacggtc ttccagcggt aagaacgacg tcggcaaacg caggaacagg aacaccatgc
    12181 cgccaagcag caggacataa atcacaatcc agcgcaggct gcggtggaga attttcgcca
    12241 cccctttttc gtagcgttcg gcgttgcggt taaacatctg gttaaaccag gcaaaaaagc
    12301 ctttttgccc gtgatgctca ccttttttca gtggcttaag tagtgtggca cacagagccg
    12361 gagtgaggat catcgctacc agtactgaca gcaccatcgc cgcaacaatg gtaatagaga
    12421 actggcgata gatggcaccg gtggtgccgc cgaagaaggc cattggtaca aataccgccg
    12481 aaagcaccat cgcaatcccg accagtgccc cctggatctg ccccatcgat ttacgtgtgg
    12541 cttcgcgagg agcgagtcct tcctcactca taatacgttc gacgttttcc accaccacga
    12601 ttgcgtcatc caccagcaga ccgatcgcca gtaccatcgc gaacatggtt aaggtgttga
    12661 cgctgtaacc gaaggcgtaa agtacggaga aggttcccat caacaccacc ggcacggcga
    12721 tagtgggtat cagcgtggcg cggaagtttt gcaggaacag atacataacg aggaaaacca
    12781 gggcgatagc ttccagcagc gttttcacca cgtcttcaat ggaggcttta acaaacgagg
    12841 ttgtttcata cgccaccttg tattccagtc cgtgcgggaa gtactgcgcc agctcgtcga
    12901 gacgattgag caccagctcc ctgtcgccat ttcgtttgcg ccggaggcca gttttacccc
    12961 cagcccggag gctggcttac cattgaagcg gctaagataa tcgtatttct ccgcccccat
    13021 ttcgacggtg gcgacatcgc ccagccttac ctctgagccg tcttgattca cccgcaaggt
    13081 gatatcgcgg aactgttctg gtgtttgcag cagtgactgg gcgttaatgg tggcgttgag
    13141 cgcctgctta tcgacggaag gtgtaccacc aagttgccca accgcaatct gcgcgttctg
    13201 tgactcaatg gcatcagtga catctttagc cgtcatctgg aaactgttga gtttcgccgg
    13261 ggccagccag atacgcatgg aatattgcga accataggca tcgatatccc cgacgccatt
    13321 cacgcggctt aacgggtcct gaatattact ggcaacataa tcggcaatat cctgtttatc
    13381 catcgaacca tcggtagaga cgaaagcaat ggtcagaata ttggtatcgc cggttttacg
    13441 caccgtcacg ccctgatttt gcaccgcctg cggtaacttt cgcatggctg attgcagctg
    13501 gttttgtact tgctgcacgg cttcgtccgg atcggtgcct gctttaaaac ttaaagtgac
    13561 agatgcctga ccggtgccac tgctctgaga tgacatatac atgagattat cgaggccggt
    13621 catattttgc tcgataacct ggggcacggt gttttccagc gtctgggccg atgcgccggg
    13681 atagttagcg gtcactcgca cattcggtgg cgcgagatcg gggtattgtt caacgggcaa
    13741 tgaaaaaatc gccagggtcc ctgtcagaca caacaggatt gccagcaccc aggcaaaaat
    13801 ggggcgatca ataaagaaat tcgccattaa aagaggacct cgtgtttctg cgtatcgttg
    13861 taggcttgac ttgcttcact gtagcggcaa ggtacgagcc aatcgtggag aaaaaaggag
    13921 ttaatgtaaa ttatcatgcc cttttatact gaaccttaag tgcaagtatt ctttggtcag
    13981 taggaggcat tacatcaact gactttcctc accctctgca gagcgaaagc taatactcac
    14041 taacgtgccg ccgccggaag gttgtgaaaa agtcagcgtc ccacccagcc gttccgcgcg
    14101 ttcgcgcatg atattcagac cataatgacc ttcgggttct ttcggttcac cgataccaat
    14161 cccgttatca cggatataaa ccgtgtgatt gccgtccggc gcggtgacgc aactgacggc
    14221 gatttcgctg gcgttggcgt gcttcatcgc attcagcacc gcttcgcgaa taatttgcaa
    14281 caaatgcacc tgcatttgcg catccagcgc aagggttggc agacggcagt cgagggtcag
    14341 tttggcgctg gtttgatttt gtaacgtatc cagcatttcc ctcaacgcgg aggggagatc
    14401 cgcctgctgc agcgtcaggc ggaaagtagt caacagctcg cgtaactgcc gataagcatc
    14461 attcaacgcc tgggaaaaat cggccatgat actttgtgcg gtggcgttat cttccggtat
    14521 cgaacgcttc agtaacgtca actggatacg taagtaagaa agtacctgag ccagcgagtc
    14581 gtgcaattcg cgggcgatgg ttgcacgttc ttccatcaac aataaatgct gaaaatgctt
    14641 ctgcgcctga ttaaagtaca aaccgcgtcc cagcatcgac gaaacgctgt taagcagcgg
    14701 ttctgatgat gaaacgtgac tattttgcca gtgcagttcg ccgtaaaccg tctcttgcat
    14761 tgtcaccggt aaaatctgca tcggcaattc cgggtttggt tgcccttcgc taatccgcca
    14821 gttttcaccg acatttaact ccagatattc agccgcttca ttgtcgcgaa caatctgcaa
    14881 aatatggcgg aaacaatgca catcaatctg gcttgtgttc agcgcctgcg aacattgata
    14941 caacacctcc agacgacgct tggcctcgtg gagatcgcgg gtcttttctt ctactgacgc
    15001 ctccagcgaa aggtacaatt tatgcagctc gctcgacatc tggttaaagg tttttgcaag
    15061 cagaccaagc tcattcggca gactggtatc cagcggcggc gagtcgaact gcccgtgttc
    15121 aatacgttga ctggcggtaa ccagctgatt cagcggggca accacctgat ggcgtatacg
    15181 gcgcaaagta aaaaagacca gcgtgaaaat accgataccg ccagccaggg aaatcgccac
    15241 caccagcagc attttgcgtt cagcgtagtg ctgtaaagcc agcacgaaca ggtctatctg
    15301 attaacataa ttattaatat tggcctgata ccacggcaaa tcgcccttgc tgagccgatt
    15361 attcatctcc agccagttgg cattcagatg cgcatagcga gtttttactg cttctggcac
    15421 ataccagacg ttgagattgg ttaataccgg tgaatgcagt gcctgctgaa acaactggcg
    15481 atgtgcattg agttgtggac tgccactttg caagtcgtag cccaggcggt aactctgcat
    15541 acgcagcgat ccggcaatat tgatagcctc agcgtcgcgc aaactgctcg ccagagttag
    15601 cagagcgata cccgtggaaa gaatcgacag cagcacaatg taaaaaaagg cccgggccag
    15661 actggccgag acgggtcgtt taacaatcac acgcgtcttc tccacaaaaa atcattaatg
    15721 ttccagttcg ctgaaacgca atttagtcag tgatgggatt atttgagctg ccacagttta
    15781 tagcgaaata gttacttatt ctgggcacat gaacattgcc gattcccctg taagtcgggt
    15841 aataacaaca ctcatataaa gaataaggtt tttacaacca aaaaagaagg tcgttatgaa
    15901 tcgttttatt atggccaaca gtcagcaatg tctgggttgt catgcttgtg aaatcgcctg
    15961 tgtcatggct tacaatgatg agcaacatgt cctgagccaa caccattttc atccccgaat
    16021 tacggttatc aaacatcagc agcaacgtag tgcagtgacc tgtcaccatt gtgaagatgc
    16081 gccctgcgcc cgtagctgcc ctaatggcgc aatcagccac gttgatgaca gcattcaggt
    16141 caatcagcaa aagtgtattg gctgtaaatc ctgcgtggtg gcctgtccgt ttggtacgat
    16201 gcaaatcgtc ctgacacccg tcgcggcagg aaaagtaaaa gccacggcgc ataaatgcga
    16261 cctttgtgca gggcgcgaaa acggtcctgc ctgtgtcgag aattgcccgg cggacgcgct
    16321 gcaactggtc actgacgtcg cactctccgg catggcgaaa tcccgccgct tgcgcaccgc
    16381 acgtcaggag catcaaccgt ggcatgccag taccgctgca caagaaatgc cggtaatgag
    16441 taaagtcgaa caaatgcagg caacgcccgc gcgtggcgag ccggataaac tggcgattga
    16501 agcgcgcaaa accggttttg atgaaattta tctgccattt cgcgccgacc aggcacaacg
    16561 ggaagcctcg cgctgcctta agtgcggcga gcacagcgtt tgtgaatgga cctgcccgct
    16621 gcataaccat attccgcagt ggattgaact ggtgaaagcc ggaaacatcg acgccgccgt
    16681 cgagctttct caccagacca acaccctgcc ggaaattacc ggacgcgttt gtccgcaaga
    16741 ccgtttgtgt gaaggtgcct gtactattcg cgatgagcac ggcgcggtaa ctatcggcaa
    16801 cattgaacgc tatatttcag atcaggcgtt ggcgaaaggt tggcgtcctg acttaagtca
    16861 tgtcaccaaa gtggacaagc gggtggcgat tatcggggca ggtccggcag ggctggcctg
    16921 tgcggatgtt ctgacccgca atggcgtggc ggtgacggtg tacgatcgcc atccagaaat
    16981 cggtggcttg ctcactttcg gcattccttc tttcaaactg gataaatccc tgctggcacg
    17041 ccgtcgggaa atcttcagcg cgatggggat tcacttcgaa ctcaattgtg aagtgggtaa
    17101 agatgtctct ttggattcgc ttttggaaca atacgacgcg gtcttcgttg gcgtaggcac
    17161 ttaccgttcc atgaaagcgg gtttacccaa tgaagatgcg ccgggcgttt atgacgcgct
    17221 gccgttcctc atcgccaaca ctaaacaggt gatggggctc gaagagctac cggaagagcc
    17281 gtttatcaat accgccggac ttaacgtcgt ggtactgggc ggcggcgaca ccgcgatgga
    17341 ctgtgtgcgt actgcgctgc gccacggcgc gagtaacgtc acctgcgctt atcgtcgtga
    17401 tgaagctaac atgccaggct cgaagaaaga ggtgaagaac gcccgcgaag agggggccaa
    17461 cttcgaattt aacgtccagc cggtggcgct tgagctgaat gaacaaggtc acgtttgcgg
    17521 gattcgtttc ctgcgcacgc gtcttggcga gccggatgcc taggggcgtc ggcgtccagt
    17581 accggtggaa ggcagtgaat ttgtcatgcc agccgacgcg gtgattatgg cgtttggctt
    17641 caatccgcac gggatgccgt ggctggagtc gcacggtgta acggtagaca aatggggccg
    17701 catcatcgcg gatgtggaaa gccagtaccg ttaccagacc accaatccga aaatcttcgc
    17761 cggtggcgac gccgtgcgtg gtgcggatct ggtggttacc gcaatggcag aaggacgtca
    17821 tgcggcacag gggattattg actggctggg ggtaaaatca gtcaaatctc actgatagcc
    17881 tgcgcagaca aacccgactt cacagcgtaa gataattgtt catttcgcgc tgtggagtcg
    17941 gtatgacgca acaaatcacc ctcattaaag acaaaattct ctccgataac tatttcaccc
    18001 tgcacaacat tacctacgat ctcacgcgca aagatggcga agttatccgc cataaacgtg
    18061 aagtttacga tcgcggtaat ggtgcgacga tcctcctgta caacgcgaag aaaaagaccg
    18121 tggttctgat tcgtcagttc cgtgtcgcta cctgggttaa tggcaatgaa agcgggcagc
    18181 tgattgaaac ctgcgccggg ctgctggata acgacgaacc ggaagtgtgc attcgtaaag
    18241 aagcgattga agagacgggc tatgaagtgg gcgaagtgcg caaattattt gaactgtata
    18301 tgtcgccagg cggtgtgact gagctaatcc acttttttat cgccgaatac agtgacaatc
    18361 agcgcgctaa cgccggtggc ggtgtcgaag atgaagatat tgaagtgctc gagctgccgt
    18421 tcagccaggc gctggagatg atcaaaaccg gcgagatacg tgacggtaag acggtgttat
    18481 tgcttaacta tttgcaaacg tcacatttaa tggactgaaa aataacaata atatttcgtt
    18541 gtttattatt ggctcaatcc gacaaatccg attgagccgc gctacctgcg caacgaagat
    18601 acgacttgtg ctgtttgttt gaacttctgg ggtcgtaccg tccatgcgct atcgcatttt
    18661 ccttctcttt tttttcgctt tgttgccgac gtctttggtg tgggcggcac cagcgcaacg
    18721 ggcgttttcc gactggcagg tcacctgtaa taaccaaaac ttctgcgtgg cgcgtaatac
    18781 gggcgatcat aatggactgg tgatgaccct gagccgcagc gccggggcgc ataccgatgc
    18841 cgttttacgt attgagcgcg gcggattgaa gtcgccggag gcgtcagaag gggagatagc
    18901 gccacggctg ctgttagatg gcgagccgtt ggcgttaagt ggtgacaagt ggcggatttc
    18961 accatggtta ttagtaaccg atgatacggc aaccatcacc gcgtttttgc agatgattca
    19021 ggaagggaag gcgatcacct tacgcgacgg caatcagacc atttcgctga gtggcttaaa
    19081 agcggcgttg ttgtttattg atgctcagca aaagcgcgtt ggcagtgaaa ccgcgtggat
    19141 caagaaaggg gacgaaccgc cgctcagcgt accgcccgcg cctgcgctga aagaggtcgc
    19201 ggtggttaac ccaacgccga cgccactctc actcgaagaa cgcaacgatt tgctggatta
    19261 tggaaactgg cggatgaatg gtctgcgctg ctcgcttgat ccattgcgtc gtgaggtgaa
    19321 tgtcactgcg ctgactgatg ataaagcgct gatgatgatt agctgtgagg caggggccta
    19381 taacaccatt gatttggcat ggattgtgtc gcgtaaaaat ccgctggcct cgcgcccggt
    19441 tcggttgcgt ttgccgttca acaacgggca ggagacgaat gaactggaac tgatgaacgc
    19501 aacatttgat gagaaatcgc gtgaactggt gaccttagcg aaagggcgcg gattaagcga
    19561 ttgtggcatt caggcgcgct ggcgctttga cggtcagcgt tttcgcctgg tgcgttacgc
    19621 cgcagaaccc acctgcgata actggcatgg gccagacgcc tggcccacgt tgtggatcac
    19681 ccgttaaaca aaatggcggg gacatgtagg ccggataaga cgctgtaggc cggataagac
    19741 gcgtcagagt cgcatccggc ctacagcgtc cggcaatcag catccggcga tcaccatcag
    19801 gcacctttca ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    19861 aagaacggga acagcttatc cgccggagca gattccccat aacctgtcat cccaacaatc
    19921 gcccctttca aaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    19981 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    20041 atatcagtcg agggcaggga aactacgcgt acattgcgac cttctcccgc taatttctct
    20101 gccgcttgca gggggatttc catctctgaa ccggtggcaa tcaaaataat atctggctta
    20161 ccgccgctgt ctttcagcac ataaccgcca cgggcaatct cttttacctg atccggcgta
    20221 cgttccactt gggccagatt ctgccttgag aggatcagtg ccgtcggtcc gttgtggcgc
    20281 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    20341 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    20401 tcgcccaggc caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    20461 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgtggc ggtatacggc
    20521 acaaagccgc cgtggtgcgc gatgccgttg gctatagcgg tcatgccaaa ttcacgcacc
    20581 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    20641 gtcaggttgc tggagccaga tccgccgaac cgccgagcaa ctcaggcagc atcggcc
//
LOCUS       DAJTYD010000058        20559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTYD010000058 DAJTYD010000000
VERSION     DAJTYD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.058"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..866
                     /locus_tag="OO585_001923"
     CDS             <1..866
                     /locus_tag="OO585_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR6629531.1"
                     /translation="WQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNV
                     YATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQ
                     DEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIING
                     AGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFT
                     LEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQPKLRG
                     NVQTAQREVAP"
     gene            863..1952
                     /locus_tag="OO585_001924"
                     /pseudo
     CDS             863..1952
                     /locus_tag="OO585_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(2040..3338)
                     /gene="exuT"
                     /locus_tag="OO585_001925"
     CDS             complement(2040..3338)
                     /gene="exuT"
                     /locus_tag="OO585_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6629532.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3821..5233
                     /gene="uxaC"
                     /locus_tag="OO585_001926"
     CDS             3821..5233
                     /gene="uxaC"
                     /locus_tag="OO585_001926"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6629533.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5248..6735
                     /gene="uxaA"
                     /locus_tag="OO585_001927"
                     /pseudo
     CDS             5248..6735
                     /gene="uxaA"
                     /locus_tag="OO585_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6818..7369
                     /locus_tag="OO585_001928"
     CDS             6818..7369
                     /locus_tag="OO585_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6629534.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7374..8618)
                     /gene="sstT"
                     /locus_tag="OO585_001929"
     CDS             complement(7374..8618)
                     /gene="sstT"
                     /locus_tag="OO585_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6629535.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9234..10199)
                     /gene="alx"
                     /locus_tag="OO585_001930"
     CDS             complement(9234..10199)
                     /gene="alx"
                     /locus_tag="OO585_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6629536.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10481..11467)
                     /locus_tag="OO585_001931"
     CDS             complement(10481..11467)
                     /locus_tag="OO585_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6629537.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11546..12229)
                     /locus_tag="OO585_001932"
     CDS             complement(11546..12229)
                     /locus_tag="OO585_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6629538.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12306..12809)
                     /locus_tag="OO585_001933"
     CDS             complement(12306..12809)
                     /locus_tag="OO585_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6629539.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12894..14030
                     /gene="rlmG"
                     /locus_tag="OO585_001934"
     CDS             12894..14030
                     /gene="rlmG"
                     /locus_tag="OO585_001934"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6629540.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14315..14629
                     /gene="higB"
                     /locus_tag="OO585_001935"
     CDS             14315..14629
                     /gene="higB"
                     /locus_tag="OO585_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6629541.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14626..15042
                     /gene="higA"
                     /locus_tag="OO585_001936"
     CDS             14626..15042
                     /gene="higA"
                     /locus_tag="OO585_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6629542.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15087..17105)
                     /gene="fadH"
                     /locus_tag="OO585_001937"
     CDS             complement(15087..17105)
                     /gene="fadH"
                     /locus_tag="OO585_001937"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6629543.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17531..19882)
                     /gene="ygjK"
                     /locus_tag="OO585_001938"
                     /pseudo
     CDS             complement(17531..19882)
                     /gene="ygjK"
                     /locus_tag="OO585_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19899..20559)
                     /gene="ygjJ"
                     /locus_tag="OO585_001939"
                     /pseudo
     CDS             complement(19899..20559)
                     /gene="ygjJ"
                     /locus_tag="OO585_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YgjJ"
BASE COUNT         5008 a         5388 c         5408 g         4755 t
ORIGIN      
        1 catggcagtt atcgcatacc ttttataatg attcattcag ccataaagaa cttggcgtaa
       61 ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt tgggatgggc
      121 aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat catgatcatc
      181 tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat ggcgttaatt
      241 tgggagccag tggtacagac gatttgctgg gtgcggtatt ggtggatgtg aaaggcttct
      301 ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca ggcagccgaa
      361 ctttgcagct tggtcaaagc gacagtgtgt tgttccctta tcctggattt cagtctggtt
      421 ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac atcattaacg
      481 gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg
      541 ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc
      601 cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga tttacacttg
      661 aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc cttaagtgtc
      721 cactgagtgt tgaaaagaaa cgcgccagca ttcgttacag cggagatgtt acttgtagtg
      781 tggtgactta ttcacaatta ccggagttga aacagcctaa attacgtgga aacgttcaga
      841 cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat tgggcttgtt
      901 tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta cgtatgattt
      961 ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag gaaataatgg
     1021 atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag ctaatggaaa
     1081 tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt ggccggtaaa
     1141 actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgctag gatgcaaggg
     1201 ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt acagaagatg
     1261 gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag cagacagaga
     1321 tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat tcgaacagcc
     1381 cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac catattgacg
     1441 tgttcttccc ggagttcgcc cacgccacgc cgagggtgca gttagacttg catccaacag
     1501 gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg tgcctgtacg
     1561 atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa gggcgaccag
     1621 ctgcagggcg cagagacggt gacttctcta tctatcgtca gggaggaacc tctaccgacg
     1681 agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg
     1741 atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaaagt gtgcgtccgg
     1801 tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg gctctggcag
     1861 tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa ttgtcagtaa
     1921 tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa cgcttttttc
     1981 tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta cgttaaacgt
     2041 taatgttgcg gtgcgggatc gttatgtgat tcctgcgcta cctcaatcgc cggtttgttc
     2101 tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag tactgcgaac
     2161 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgaaaa cagcgtactt
     2221 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
     2281 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgagcaaa accaccgata
     2341 cacagcagca taattgcgac atacgggttg gtgaacaggc cgatcatacc cgggccaatc
     2401 atcagcactg cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
     2461 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
     2521 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
     2581 aacagcggga tccacgcgtt aaaagtaccc caggctggtt ctgccaggaa acgcggcagc
     2641 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
     2701 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
     2761 tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
     2821 aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc ccataccacc
     2881 agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc taccgcgatg
     2941 gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg gatcatcgct
     3001 gcttccgcgg caccgactgc accacgagca atcgccaggc cacccctgct accagccagc
     3061 gcggttgcac cacagaatac ggcccacagt acagcaaaca ttgcataacc gattttcgta
     3121 cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc agcagaatag
     3181 gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat cagagttggc
     3241 gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag cgtcaccagt
     3301 gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat cgttttgtta
     3361 tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt tacgaccggg
     3421 taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg ctaattcggc
     3481 ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt tgcattccgt
     3541 cgacttatga tttgcgacgg cagaaagatg acttgtcata caactttaaa aggtgagagc
     3601 catcataaat gtgcgaatat ttgtagggac attacctgac gacagcgagg ccagtactgg
     3661 cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg tttattgatc
     3721 taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt cagcatcgca
     3781 ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt ttatgactga
     3841 agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg caaaagacca
     3901 gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag actatcgttt
     3961 taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg ctatgcgtac
     4021 caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa aatttgacgc
     4081 ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga cgcacctcga
     4141 actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg ccgatgaaat
     4201 ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg gcatcatgca
     4261 gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc tggagcatca
     4321 cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct ggcgtccgga
     4381 caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc tgggcgaagt
     4441 ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca aacgtctgga
     4501 tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg tgatgtttgc
     4561 tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg gcgaaaccct
     4621 gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg gtgccgaata
     4681 tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata acaacctgcg
     4741 tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc gtccgatggc
     4801 agaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc cgaaaaccat
     4861 tctgtactgc ctgaacccgc gcgataacga agtgttgggc accatgatcg gtaacttcca
     4921 gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta acgatcagaa
     4981 agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga gccgctttgt
     5041 cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat acttccgccg
     5101 cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa
     5161 cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact acttcgccat
     5221 tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc gctggataac
     5281 gtcgcggtcg ctttagcgga tttggctgaa ggcacagaag tcagtgtcga taaccagact
     5341 gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa
     5401 ggggccaatg tcatcaaata tggcctgccg attggttatg cattggcgga tattgcggcg
     5461 ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga tcagtatcgc
     5521 tatcaacctg attttcagga tctgcctgcg caagcggcag atcctgaagt gcagatctat
     5581 cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc aaccgtgggc
     5641 tgtgtcaacg gcatcgcgcg gcagatccag aaccgttttc tgaaagagac caacaacgct
     5701 gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca gctgggcgac
     5761 gatcacatta atacccgcac catgctgcaa aacatggtgc gccacccgaa cgcaggcgca
     5821 gtgctggtga ttggtctggg ctgtgaaaac aactaggttg ccgcattccg tgaaacgctg
     5881 ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga tgatgagatc
     5941 gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga taaacgcgag
     6001 ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga cggtctttct
     6061 ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc taacggcggt
     6121 actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct gatggaccat
     6181 tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt taaacagtac
     6241 tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa agcgggcggt
     6301 atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc cagtgtcgtg
     6361 gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt gttaagtgcg
     6421 ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg
     6481 ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa aatcgccacc
     6541 aacagtgaac tggcggcgaa gaaaaaacac tggctcgact ttgacgcggg tcagctgatc
     6601 cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt tgagtttgcc
     6661 aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat cttcaaaagc
     6721 ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa
     6781 gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt ggctggccca
     6841 gggcgtgggt ttcatcgcct ttctgattgg tatcacaaca tttttcaatc gtgacgaccg
     6901 tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt tttttcttct
     6961 gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat tgattacctt
     7021 acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg gaattggcct
     7081 cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg tcagtaccag
     7141 ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg
     7201 ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg agggtagttt
     7261 tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa ggggaattga
     7321 tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa caattaatta
     7381 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
     7441 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gatgataaag
     7501 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagc
     7561 gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca cagagaagcc
     7621 accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt attaaccgca
     7681 gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt ggcacccagc
     7741 ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag tgccatattc
     7801 accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct ttcgcgcagg
     7861 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
     7921 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
     7981 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa aataccaatc
     8041 ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacag atcgttgacc
     8101 aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat tgcccacacc
     8161 aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac catgctcatt
     8221 accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc cgcgctactg
     8281 gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag agcagcagag
     8341 aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg cccgtgctgg
     8401 tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac ggctttcagt
     8461 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
     8521 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct gccatgagcc
     8581 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
     8641 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
     8701 aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat tttaaagaga
     8761 ggtcatattt tgcaacttaa ttgttacatt tgtttgacat aaacagaggg aaggactggc
     8821 tactaaggct gattagataa catatgggca aaactatttg gaatatgaga acggttgcgc
     8881 tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag ggggcagcca
     8941 atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct gcgcgattcc
     9001 ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg taagatgcgc
     9061 cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg cgattttgac
     9121 gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccaattaca taattaatag
     9181 ctcacctttg tctgggatga gttatctcta ggaatggctt aatatacagc gaatcattct
     9241 ccccgctgct tatcatgccg ataattcacc ctggcgttga taataaacgt catcaccaga
     9301 atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc gacaatcagc
     9361 atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat cgagaaacgc
     9421 tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc aaacaggttt
     9481 gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat gctatccacg
     9541 gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg tgtggcgtac
     9601 agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt catgcgcaaa
     9661 tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc ttcatgggcg
     9721 agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag gatccagtcg
     9781 aactgcgaaa ttagccagct gccagtgaag atcatgatgg tacgcagaac aatcgccccg
     9841 agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa atagctgaac
     9901 aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag ataacccgtg
     9961 agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac cagataccac
    10021 cagaaagcgg cgttaaacag taacgacagc gtcacccaaa ccagcgacca ggccgcagcc
    10081 tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc gatagccagc
    10141 ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac agtattcata
    10201 gaagttcctt acacataaaa aaaacggcca acgtcggaag acgtcagccg ctgcttttta
    10261 tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaagaata cgtctgcgta
    10321 ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat gacgatcgac
    10381 cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagccattc agccagtcaa
    10441 tggtctgtta catacctttt acactgtttt actttggcaa ttatagtttt acgctatctg
    10501 ccggaaaaat caccccagtc tggcggcgga tctcagtcag cagtttggcg gtgatgcgac
    10561 tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagca aacagctctg
    10621 cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac
    10681 gcggcacgaa gcacactttc tggcattcag acagtttttc aatcaccagc gatcccgctt
    10741 cgccctgaat ctcgctttcc aggacagaat cactgacctt tgagtgcagc aacgtaacgc
    10801 tgaaatcacc gtaatccatc accaccacgc cgtgggcatc tacgccgctt gccaacaaac
    10861 tggcggttgc ctgcacgctt ttcggttcac cgaataatgc caccgccgac gccaggcagt
    10921 aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct
    10981 caccatccag gtaacgttga taccgcgagg aatactggca atagttgaaa aagactttac
    11041 gcaatttgcc gactttcggc agcgcctggc gcagcaaatg aaagttcggc aggctggcgg
    11101 ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg gcggcatcca
    11161 cttccgccag attcgacgcc agtggtttct cgcaaatcac atgaatttta tggctaagga
    11221 aaagttgtgt ctgggaaaag tgcagggcat tcgggctggc aatatacacc gcgtcaatgg
    11281 catcgctttc cgccatcgct tccagcgagg taaacagatg ctcgacagaa aaatcattgg
    11341 cgaagtgctg ggcctgttca aggctgcggg aatatacggc ggttaacttg tatttaccgc
    11401 tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc acagcgaaac
    11461 gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc acgccttttt
    11521 catcttcgac gtgcgcagat acgcattatt cttttgccgg acagccatgc tcactaaacg
    11581 gcccaatcat gaccgatggc ccgagatatt tcggctggct atggaggatt ttggcgtcaa
    11641 tcacggcgct gacacgagcg agtttctctc gcagttgagt ataaaaaccg tgcttcatat
    11701 tgaggtcagg cgcgttaaaa ccgatagcgt cgatgctgta ctgttttgcc agccagatgg
    11761 cgcgttggtt atgaaattct tgcgagataa tggtgatatg gttttcacca aaaacctttt
    11821 tggcacgcac taccgaatcc agcgttgaga atccggcata gtcgcagaag atcactttcg
    11881 ccggtacgcc tttagcgatc aaagccttct gcatacctga tgcttcatcg taatttttac
    11941 gtccgttatc gccacttacc agtagccatt tcacttttcc ggcgtggtac aacgctgctg
    12001 cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc aataagccca
    12061 cgttacgcgc cggaactgcg ttgacatcgc tccaggtcag ctgcttgctg gcgttgacca
    12121 ttactcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag caggcaatct
    12181 ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg gggctgcatg gtcgttatcc
    12241 ttaacgaaag gggggaggtc gattttaggc gctaaagaaa gaaaagataa cgcctgacat
    12301 cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg ggtgtcgaac
    12361 tccagctggt ggtactgcgg ctccatatgg cagcacaact ggtaaaacgc tttgttatgc
    12421 tctttctcct tcaggtgcgc cagttcgtgc accacgatca tacgtaaaaa cggttccggc
    12481 gcgttgcgaa acacagtagc gacgcggatc tctgcttttg ctttcagctt gccgccctgc
    12541 acacgagaaa cagcggtatg taacccgagc gcgtttttta gcacatggat cttattgtca
    12601 tacatcactt tattgatcgg cggggcattg cgcagaaact gattttttag atcctgagta
    12661 tattgccaga gggctttatc ggtggcgtag tcgtgtgttc ccggatagcg ttttgccagc
    12721 acatcgccca gacgctgttc gttaatcagc gttcgcacct gggaaagtag ctgctcggga
    12781 taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa aaagggtata
    12841 ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg ccgatgagcc
    12901 acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg gatgacgtta
    12961 acccgctaca ggcgtgggaa gcggcggatg aatatttgct gcaacagttg gacgacacag
    13021 aaatccgcgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagc tgcgcgctgg
    13081 cggaacataa gccgtacagc attggcgact catacatcag tgaactggcg acgcgcgaga
    13141 atttacgcct caacgggatt gatgagtcga gcgtgaagtt tctcgacagc accgccgact
    13201 acccgcaaca gccgggtgtg gtgctgatca aagtgccgaa aacactggca ttgctggaac
    13261 aacaactgcg tgcgctgcgc aaagtggtca cgtcggatac acgtattatt gccggtgcca
    13321 aagcccgtga cattcacact tccacgctgg aactgttcga aaaagtgctc ggcccgacca
    13381 ccaccacgct ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat gaaccgccgc
    13441 tggccgatgc gccgcagacc gttagctgga agctggaagg tactgactgg actatccata
    13501 accatgcgaa tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc tttatgcaac
    13561 atctgccaga gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat ggcgttattg
    13621 gtctgacgct gcttgataaa aatccgcagg cgaaagtggt gtttgtcgat gaatcgccga
    13681 tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gccagaggcg ttggatcgca
    13741 gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgcttt aatgctgtgc
    13801 tctgcaaccc gccgtttcac caacaacatg cgctgaccga taacgtcgcc tgggagatgt
    13861 tccaccatgc ccgccgctgc ctgaaaatca acggcgagct gtatattgtt gccaaccgtc
    13921 acctggatta cttccataaa ctgaagaaga ttttcggcaa ctgcaccacc atcgccacga
    13981 ataataaatt tgtggtgctg aaagcagtga agctggggcg tcgtcggtaa gattgggagt
    14041 tacctgccgg atgcggcgtt aacgccttat ccggcctacg ttcagcacgt tactcccagg
    14101 ccggataaga cgcggcaagc gtcgcatccg gcaatgtctg cccagttctg gaatcgcgct
    14161 tccataaaac aaaaaatccc tctcaccatc ctctacattc tcttgtttag cgtttttcta
    14221 cgtttattct tccgtcacac agataaattc atacgttgcc aatctgtcaa cggatgttat
    14281 catattgcca atttgtcaac ggacgtgatg aagaatgcac ctgataactc aaaaagcatt
    14341 gaaagatgct gcggaaaaat acccgcaaca taaaacggag ttggtggctc tggggaacac
    14401 gattgctaag ggatatttca aaaaacctga gtcattaaaa gcagtattcc catctctgga
    14461 taacttcaaa tatctggata agcattatgt tttcaatgtt gggggcaatg aattacgtgt
    14521 tgtagcaatg gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta tgacgcataa
    14581 agaatacgat ttctttaccg ctgttcatcg tactaagggg aaaaaatgat tgcgattgcc
    14641 gacatcttgc aagcaggaga aaagctaact gcggtggtac cttttctggc gggtattcag
    14701 aacgaggaac aatacaccca ggcgctggaa ctggtagatc atctgctgct caacgatcct
    14761 gaaaacccct tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga atcagcgccc
    14821 gaatttgcgg aatttaatgc catggctcaa gccatgcctg gcggtatagc cgtgattcgt
    14881 acccttatgg atcaatatgg tttaaccctt gccgatctgc cggaaattgg cagtaaatct
    14941 atggtgtcac gcgttttgag cgggaagagg aaattaacgc tggaacacgc taaaaaattg
    15001 gcaacgcgat tcggcatttc tcccgccttg tttattgatt aacgcgacgt gccggatgcg
    15061 ttgcatccgg cgcttcaaat aacgatttaa atctccagcg ccagccgtgt tccctgggca
    15121 attgcccgtc gtgcgttcag ctccatagcc acatcgcagc cgccaattaa atgcacagtt
    15181 ttcccgctat caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc tgcgcagatc
    15241 accacattgt ccacagctaa tacctgcgtt tcgccgttga tcaccacatg cagcccgtca
    15301 tcgtcaatct tctgataact tacgcctggg atcattttca caccccgcga gagcagggtg
    15361 gtgcgatgga tccagccggt ggttttgcct aacccctgtc ccggtttgct ggctttgcgc
    15421 tggagcatca caatttgccg tgggctacgc gggatctgca ttccctgcgg gcttaagcca
    15481 ccagcctgtt gtaggctgct gtcgatcccc cactcattac agaacctggc gatattctgg
    15541 ctggttgatt cgcccggctg acttaaatac atcgccgtat caaaaccaat cccgccacaa
    15601 ccgatgatgg caaccttgtt gcctaccggc gctttgtcgc gcagtacatc gagataactc
    15661 aataccttcg gatgatcgat cccgtcgata ggcggaacgc gcggcacgat cccactggcg
    15721 aggatcgttt catcgaacgc ctgtaactga tccgccgtca cggtgtgatt gagtttcagc
    15781 gtcacgcccg tcacttcgat catccggtgg taatagcgca gcgtttcgta aaactcctct
    15841 ttgccgggga tctgtttggc gatattaaac tgcccgccgc tctcgctgtg ggcatcaaac
    15901 aatgttacct gatgcccacg cgccgccgcg ttaatggcaa acgccagccc agcaggtccc
    15961 gcaccgacca ccgccagatt ttttttctgc acggcaggaa ggattggcat tttggtttcg
    16021 tggcaggcgc gaggattcac caggcacgag gtgactttgc caacgaagat ctgatcgagg
    16081 caggcctgat tgcagccaat gcaggtgttg atctcatcgg ctcgccccga ttgcgctttt
    16141 gacagcagtt ccgcatcagc aagaaacggt cgtgccatcg ataccatatc ggcatcgccg
    16201 cgcgagagaa tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt taccagcggc
    16261 agcgagacgt ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg cggcacgggc
    16321 gtggcaatgg tcggaatacg tgcttcatgc cagccaatgc cggtgttgat aatggtcgcg
    16381 cccgccgctt caatggcctg cgccagctct accgtttcgg caaaagtccc gccgtcttcg
    16441 accaggtcga gcatcgacag tcggtagata ataatgaagt cgttgccgac gcgttcgcgc
    16501 accgcacgca ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc gccccactgg
    16561 tcactacgct gattggtgtg cagcgtcaga aattcgttga tcaaataccc ttcggacccc
    16621 attacctcta caccgtcgta tcctgcctcc cgtgccagtt gcgcgcagtg ggcgaaatcg
    16681 tcgatcagtt gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg gttgatgggg
    16741 gcctgcaatg cggagggagc gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa
    16801 atttgcaggg ctattttgcc gccttcctga tgtaccgctt cggtaatggt gcgatggtgt
    16861 gggatctggc tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc tgttaaatct
    16921 ggtgcaatgc cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac
    16981 gctgccagcc gctcggcacc gtccgggtat tcctccagcc cggtgtgcat tgagcccatc
    17041 aacacgcggt tttttaacgt ggtaaaacct aaatccagcg gggcgaacag cgacgggtag
    17101 ctcataaaat tgtccagtat gtaaaataat tgttatgtgg tcggatgagt tctaatttag
    17161 ccgtcgccgg agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat
    17221 acgcccggtg gtaacgccgg atgcgacgct tgccgtgttt tatccggcct gggggattgc
    17281 acatgtaggg cggataaggc gttcacgccg catccgccag tggcgcggtg cagatgccgg
    17341 atgcgacgct tgtcgcgtct tatccgacct acgggaacgc acatgtaggg cggataaggc
    17401 gttcacgccg catccgccag tggcgcggtg cagttgccgg atgcaacgct tgccgcgttt
    17461 tatccggcca ggaggattgc acatgtaggg cggataaggc gtttacgccg catccgccag
    17521 cagttgcaaa ttattgctta cggaaaaaat cgttatacaa catatacaaa tgcgcggcac
    17581 tccaggagaa atttggtgcg ccttgctgcg cgccagtcag cgggttgtaa ttctcctgaa
    17641 ttgggccatc ggcggttaat cctttggcgt gccggaagaa cgtatccgcc agcttcaggg
    17701 catcatcacg ataaccgtaa cgctccatcc ctttcagagc aaaccagaac tgatccaccc
    17761 atacgcgccc gcgccagtag atatcagcgc caaaagccgg attggttaac gccgccgttc
    17821 ccagcgggac aaaggtgttg aactctttag gatcgagcat caccttcacc accgcgtcgg
    17881 cattggcctg cgttgccgca ccgttaaaca gcggcgacca gccttccggc cctttaccgc
    17941 gctcaacaat cggtttgccc gcgcagccgt tcgccagtgg tttatcttca atacgtacgt
    18001 catagtagaa ctgggtagtc gggtcgaaca tacaggtgtt gatgtagtcc gcgagctgct
    18061 gtgccaactg gcgatagcgt ttggcctctt ctggcttacc gaggatggtt gccatctccg
    18121 ccagataatg gttatcgctg tacatatagc tggcctgatc caccgactcc tgcaatagcg
    18181 agtagcccag caacgttccg tcctgactgc ggttttcggc gaatttcacc gtccagtcgc
    18241 tacgtttgcc accattagcg acatatttat ccaactgttc tttgtcgata aaaccaaaga
    18301 cggcggcatc atcacgcccc gattcccacg acgcagcgac ctgtgccgga atttccagac
    18361 tgtcgtactg gcctttctcc accacgcggg cgtagttgtt cagcccagac tgcgtctctt
    18421 ctttgttgcc tttcttcacc gcaaacagca tctcgccgct ctcagtgttg tgggctttgt
    18481 cgcgggtcgc gccatattcc ggcacgccgt tgccgttgtg atcgcggtta cgtaaccacc
    18541 agtcgtgata ggcgaccagt ttcgggtaca tctctgccaa ccaggcttta tcctgggtga
    18601 cgttgtacac ttctatcacc gaccaggcgg caaggctggg tttggtatta cgttcgttcc
    18661 agttgccgcc atcgccacca cgctcggggc taaggttcca cgctatcagg tcggggacaa
    18721 agcccacatc ctgcggacgc acgcggtcgc caggctggat ctgccaggag aagaccgcgc
    18781 ggatattctc tttggcgatg tccggattga aatgcgccat cgcaaacgcc tgcttctagg
    18841 tatcccacgg ccaggtctga ttgccggaga accagcgccc ggtcaccgac ggtgtaacgg
    18901 tgttaaattt caccgcaccg cccggtgagc gccagttacc gttgagcgtt tcgatcgctt
    18961 tcaccgcgac gcgcgtctgt tccggcgtcg catccggatt ggttaaccct ttcttcaggt
    19021 attcttccca gcgttgctgc gaggcggtga gataaaacgc cggacgcgcc agaatatcgc
    19081 ggatctgcat ttgctctttg ctaacttcct gagcggtcag cagatgggaa taggtggtgt
    19141 agagcgtggt cgaaccgttg atatgcgcct tactggtaaa gcgattaccg ttgatttcag
    19201 tctgcaccgg cagggattta tgcacctgat attccgattc gccggaggtc agcagatccc
    19261 aggtggcgcg cactttgcca aaggtgactt tcaggccatc acgggtggcg ctgattttgc
    19321 gctgatagtc agggtattcg ccagcaatgg ttttatcgga aagcggtttc ccttctttcg
    19381 cttccagttt ttccagcagt tcgccgtccc acaccagatc cagcggttta ttgctggtga
    19441 ttttggtttc cagtagtgac gtgcgcggcg tggcgaagcg cagagtcatt tcgacttgca
    19501 catcttttgc tgtcagtttt tgcaccagcg caccgggaat actgtatgcc tccagcgtaa
    19561 agtcgacttt cttgccgtcc tgccagacgg tcaggcggtc gaaattgctg gccataaagt
    19621 tgatgtactc ttccgtcagc agcgcaacgc ccggaaagcc gcccatggtg ttagggccgt
    19681 ctggcaacag atgaccatgc caggctccga gatcgaaaaa cggattaaaa cgctggtgat
    19741 cgtcgtaatc gtaatccttc atgtactgcg gcgcgccagt acggttaatc acgtttttat
    19801 aattgtcggc gttagcggca tgggcggaaa aacttatcag cagagcgcag gttactggcg
    19861 ttaaaatagt ttttattttc atgaaattac agtccttatt accagtagaa atattgcatc
    19921 aggaagattt ggtcgctggg atcgtgagag gcatttttca tgtagaagcg gctatcaaaa
    19981 gccgtggcga aacgtccgcc gccgtattcg taccagatac caaactgcat gaaagaggtg
    20041 gtttcatcgt cagcgtggtc agggcgatgt ttggcgtagg tgtaagcggt ggagagataa
    20101 acgcccttta acgcttcata catggcgctc accatcatcc cactttcact tcccggtacg
    20161 tccagcccgt cggcgcgtcc ggtatggtgc caggcgcgag cggcaaagtt aggagttagc
    20221 aggcccgtga gatagagctg tcctgtgccg tcggtgattt ccagaccgtt catccaggtg
    20281 atgccttcgg caaggtcata ctggatgtaa ccgttcatca ttgccggata ggtgtatttg
    20341 tcgtcatagc gatcgtattt gccaaaatgt agccaggctt tgctttcatc atgatgtccg
    20401 gcaggagtcg cggtgacgct ataacgaaag ttgccgctta agttctgtac cttcagcgca
    20461 gccataatgt cgcgggtatt gggaatgacg tagcccagat cgggggtgaa atcgccccac
    20521 cattgcaggt catccagcga ggaatcctaa cgcagactg
//
LOCUS       DAJTYD010000059        20055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTYD010000059 DAJTYD010000000
VERSION     DAJTYD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.059"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..447
                     /locus_tag="OO585_001940"
                     /pseudo
     CDS             1..447
                     /locus_tag="OO585_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            469..1431
                     /locus_tag="OO585_001941"
     CDS             469..1431
                     /locus_tag="OO585_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6629544.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            1428..2475
                     /locus_tag="OO585_001942"
                     /pseudo
     CDS             1428..2475
                     /locus_tag="OO585_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(2567..3916)
                     /gene="accC"
                     /locus_tag="OO585_001943"
     CDS             complement(2567..3916)
                     /gene="accC"
                     /locus_tag="OO585_001943"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6629545.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(3927..4397)
                     /gene="accB"
                     /locus_tag="OO585_001944"
                     /gene_synonym="fabE"
     CDS             complement(3927..4397)
                     /gene="accB"
                     /locus_tag="OO585_001944"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6629546.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5375..6349)
                     /locus_tag="OO585_001945"
     CDS             complement(5375..6349)
                     /locus_tag="OO585_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6629547.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6501..8441
                     /gene="csrD"
                     /locus_tag="OO585_001946"
     CDS             6501..8441
                     /gene="csrD"
                     /locus_tag="OO585_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6629548.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8746..9789
                     /gene="mreB"
                     /locus_tag="OO585_001947"
     CDS             8746..9789
                     /gene="mreB"
                     /locus_tag="OO585_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6629549.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            9855..10940
                     /gene="mreC"
                     /locus_tag="OO585_001948"
     CDS             9855..10940
                     /gene="mreC"
                     /locus_tag="OO585_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6629550.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            10940..11428
                     /gene="mreD"
                     /locus_tag="OO585_001949"
     CDS             10940..11428
                     /gene="mreD"
                     /locus_tag="OO585_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6629551.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11437..12030
                     /gene="yhdE"
                     /locus_tag="OO585_001950"
     CDS             11437..12030
                     /gene="yhdE"
                     /locus_tag="OO585_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6629552.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12020..13489
                     /gene="rng"
                     /locus_tag="OO585_001951"
     CDS             12020..13489
                     /gene="rng"
                     /locus_tag="OO585_001951"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6629553.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13557..17357
                     /gene="yhdP"
                     /locus_tag="OO585_001952"
     CDS             13557..17357
                     /gene="yhdP"
                     /locus_tag="OO585_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6629554.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17513..18958
                     /gene="tldD"
                     /locus_tag="OO585_001953"
     CDS             17513..18958
                     /gene="tldD"
                     /locus_tag="OO585_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6629555.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19086..20015)
                     /gene="aaeR"
                     /locus_tag="OO585_001954"
     CDS             complement(19086..20015)
                     /gene="aaeR"
                     /locus_tag="OO585_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6629556.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
BASE COUNT         4762 a         4906 c         5497 g         4890 t
ORIGIN      
        1 atattaatca tcttattgct gattaaccac ttcattctta ctaaaacaac ctttggacga
       61 aaaatttata tcactggtgg taataaagaa gcggcaattt attcagggat aaatgtcacc
      121 cgcttgaaaa ttaaagtctt tatgattaca gccgtgctgg caggtatcag cgggatgatc
      181 ctcgcctcac gcctctattc cgggtaaccg aattccgcgt tgagctacga actggatgct
      241 attgctgcgg ctgtactggg aggcaccagc ctgaacggcg gctatggaac cgtggtgggg
      301 acggtgatcg gcgcattaac tatcggcgtt attaataatg gcatgaactt aatgaatgtt
      361 ccctattttt accagatggt ggttaaagga ttagtcattc ttgtagccgt ttattttgac
      421 gtaagaaata aaagaaaacg cagctaatca tttcaaggaa ttaatcatat gagtaaggtc
      481 ttcacaattg gagaaattct ggttgaaatt atggcgagta aaatcggcca gccatttgat
      541 caaccaggta tctggaacgg cccttatccc agtggtgcgc ccgctatttt tatcgaccag
      601 gtgacacgtc ttggcgttcc gtgcggcatc attagctgtg tggggaatga tggctttggt
      661 gatattaata tacatcgtct ggctgctgac ggtgtggata tcagggggat ttcagtctta
      721 cctctggaag ctaccgggag tgcctttgtt acttatcata actctggcga tcgcgatttt
      781 atttttaata ttaagaatgc cgcttgcggt aaattatcgg cgcagcatgt tgatgaaaat
      841 attctgaagg actgtaccca ttttcatatt atgggctctt ccctgttttc gtttcatatg
      901 gtagacgcag taaaaaaagc ggtaactatc gttaaagcaa atggtggtgt tatttctttc
      961 gaccctaata ttcgtaaaga gatgctcgat attccagaaa tgcgtgacgc cctccacttt
     1021 gttcttgagt taacggatat ctatatgccc agcgagggcg aagtgttgct cttgtcgcca
     1081 cattcgacgc cggagcgtgc aatcgcaggt tttcttgaag aaggtgttaa agaggtgatt
     1141 gtgaagcgcg gcaatcaagg tgccagttac tactccgcga atgagcagtt tcatgttgag
     1201 agctatccgg tggaagaagt tgacccgacc ggtgcgggag actgctttgg tggcgcgtgg
     1261 attgcctgcc gccagctagg gtttgacgtc catcgagcgc tgcaatatgc caatgcctgc
     1321 ggtgcgctgg cggtaaccag acgtggccca atggagggaa cgtctcgcct tgcggaaatc
     1381 gaagcgttca tccagcgcca tgacatgtcc attcgggaag cagcacaatg aaaaacgaaa
     1441 tttggtcgtg gattggcgat ctctcgcagg ttgccggaat taaacactat gagttacgta
     1501 gtggcagagc aaagggtaca gaagcgtttg atgtccgtac tggtgcggga ctggcattta
     1561 cggtggtaaa agatcgcgcg ctggatattg catgggccag ctacaaggac acggcgcttt
     1621 cttttatcac ccccaacggc attgtcgccc ccgctttttt tgagtctcag ggcaacggat
     1681 ttttacgtag cttttacgcg gggttactga ctacctgcgg acttagctac attggcacgc
     1741 cttgtgagga tgaaggagaa acgctggggt tacacgggcg tttggctgcg actccagcag
     1801 aagaggtggg gtatcgcaca gaacggaccg acgacggcat tgaatttgtc atcaatggca
     1861 aagtacggga aacgcgactg ttcggcgaaa atctgacgct ggaaaggacc attcgctgcc
     1921 gttatggcga aaatgtgctg cgcattgaag acaaggtgac taatcatggt ttcacccgcc
     1981 agccgctgca aattctttat cactttaatt acggctggcc tttattatcg ccacaggcca
     2041 gaaatcttgc tgtcggctaa aagcgtaacg ccacgaactc cgcatgcggc ggaagggtta
     2101 accagtcatc tggagatttg taccccacag cccggttttg acgagcaggt ttactatctc
     2161 acgttgaata gcgacagtca ggggatgagt aaagttgctc tggtgaacgc cgaactgggg
     2221 tggggaattt acgagaaatt cgacaccatg cagctaccca actttatcca gtggaaaaat
     2281 ttaggcgcgg gtgagtatgt tatgggactg gaggtcagta atagttttcc tgacggtcgt
     2341 gataaagaac gagcgcaggg gcgtctgccc tttattgagc caggggaaac aaagaaatac
     2401 tgctttgagc tgggaattgt tgatggcgat gctgaaatga gcgcgttgaa agcggaaatt
     2461 gcaggctacc gttagctaac ggcaataaaa acgccgggca gcgttaacag gcccggcgtt
     2521 gattacgttc tttgctaagc gcgatgaaaa caccgcgcgt cacaaattat ttttcctgaa
     2581 gaccgagttt tttctccaga tagtggatgt tagtgccacc atgctggaag ttctcgtcat
     2641 tcatgatgcg gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc agctcctgca
     2701 gcgcattctt catgcgagca atcgccacgt cacggttttc accgtagcaa atcagcttac
     2761 cgatcattga gtcatagtac ggcggtacgg tgtagcccgc gtagatatga gactcccaac
     2821 gcactccaaa accgccaggt gcgtggaaac gggtgatttt gcctgggctt ggcaggaagg
     2881 tgttcggatc ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg tgaacttctt
     2941 cttgcttgat cgacagcggt tgaccggcag cgatacgcag ctgttctttg atcaggtcaa
     3001 cgccggtgat catttctgta accgggtgtt ctacctgaat acgggtgttc atttcgatga
     3061 aatagaactc gccgttttcg aacaggaact cgaaagtacc tgcaccgcga tagccgatat
     3121 caacacacgc tttagcgcaa cgttcgccga tgtagcgacg tagttccggg gtaatgcccg
     3181 gtgctggtgc ttcttcgacc actttctggt ggcggcgctg catggagcag tcacgttccg
     3241 ccagatagat agcgttgccc tgaccgtcag ccagtacctg aatctcgacg tggcgaggat
     3301 tttccaggta tttctccatg taaaccatat cgttgctgaa agcagctttc gcttccgcac
     3361 gggtcatgga gatggattgt gccagttcag cgtcgccgcg cactacgcgc ataccgcgac
     3421 caccaccgcc gccggaggct ttgataatca ccggataacc aatgcgttta gcaatggcac
     3481 ggtttttatc catatcgtcg cccagcgggc cgtcagaacc cggtacgcaa gggacgcccg
     3541 cttttttcat cgccgcgatt gcggatactt tgtcgcccat caggcgaatg gtttctgctt
     3601 tcgggccaat gaagataaag ccggagcgtt caacctgctc ggcaaagttg gcgttctcgg
     3661 agaggaagcc gtaacccgga tggattgcta ctgcgccggt gatttcagcg gcgctgatga
     3721 ttgccgggat gttcagataa ctttttactg acggagcagg gccaatacag accgtttcat
     3781 ctgccagtaa tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg acagtcttga
     3841 tgcccagttc tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa
     3901 caattttatc cagcatgttc gcctcgttac tcgatgacga ccagcggctc gtcaaattct
     3961 accggttgtc cactttcgac cagaattgct ttcacggtac cggatttgtc cgcttcgatc
     4021 tggttcatca ttttcatggc ttcaacgatg cacagggtat cgcccacgtt gactttctga
     4081 cccacttcga tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt accaaccatc
     4141 ggggagcgta cgatgtgacc actgatttcc gctgctgctg gcgcttccat ggaaggaacg
     4201 gtcgccggag cggctgcgtt agattgagct ggctgctgca tcattggtgc agcgtaagct
     4261 tgttgcatca cagggaaact tgcggcagga gctgcacggc taatgcgtac tgactcttcg
     4321 ccttcagaaa tttccagttc ggagatgcct gattcttcaa ccagctcgat cagtttttta
     4381 atcttacgaa tatccatgag tgggttccgt actctttgtt tagtgtgatt gtgacaggcg
     4441 tttcaccgcc gtctgtaaag cgtatgcccc gacagctgta tgcatagcga taaattccag
     4501 caggccggga cgccggtcta ttttgcccca gagtatcaat gttagacttg acggacattg
     4561 tgcagcgatc tgctgctatc ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt
     4621 caccttttcc gcagcgcaaa aactgcgtca gggaggacga ggtcgcacat tataacgatt
     4681 tcgtagcaat tggcagctaa atactggtct tatcagggaa gataatcaac agctaacatg
     4741 taaataacct tcaacaccgt gtaatttgca acaagccgca caattcacga aattagcgcc
     4801 atcatcgacg gaacttctta taacgtaagg tgaaaagctg taaagccagc cctgcgaaga
     4861 tgaggggctg caatgagata atcttcacag accacaaata atgtagggat gccaggatcg
     4921 cgacaagata gacgacatta cgcagcggtt gctggggatt acccaatttt cactgcatcg
     4981 tctggataga tgtgaatgct aaagcacgca aaatcaaccg actgacaatt ctcagcatta
     5041 aataggttcg agtggttaat ttgcagtcaa ataaaatcag attattcacc ccatggtacc
     5101 agaaatcagt aacaggttaa gcgtagtgtc gcccaggcaa agcaccataa acctaacagg
     5161 cgacggaggc gtatcaataa tggctgttta gaggagtatc aatgcgcatc aatttgtggt
     5221 cagtcgaaat catgactttc cagtttggat gatgttgtac aacttcctga ccaaggtgtt
     5281 cgacgaaagc tgtccggcta accgcttttc ccgttaaccg attgaataat gatgtcaaaa
     5341 tgagttccct ctcttattat tccttgctaa atggttagtt aaccttcacc agcgtgcgac
     5401 cctggatctg gttattaatg atggcctcgg caaagttcgg tgcctctgac agagatatct
     5461 cttttgccgc ctgggtatag aatgattccg gtaaatcggc gaccagtcgc tgccaggctt
     5521 gtgcgcggcg ttctggtggc gtcattactg aatccacccc ttgcaaacgg acattacgca
     5581 gaataaatgg catgaccgtg gttggcagag taaaaccacc cgccagacca caggccgcca
     5641 cgcagccgcc gtaattcatt tgcgccagca cttttgccag cactttgtcg ccaacagtgt
     5701 caattgcccc agcccagact tgtttttcca gaggacggga ttcggcaaac tcatcacgag
     5761 ggagaatacg gctggcacct aaacttttca gatattcatg ggtactttcg cgaccggaaa
     5821 cggcaacgac ctgataaccc aacttatgca gcagcgccac ggcggtactg ccgacgccac
     5881 cactggcacc cgtcacgaca atctccccgt cctgcgggcg aacaccggca tcttccagcg
     5941 ccatcacaca cagcatggcg gtaaaaccgg cagtaccgat aattattgct ttacgcgcgt
     6001 ccagcccttg cggcatggca accagccagt cacctttcac tcgcgcctgc tccgccagcc
     6061 caccccagtg gttttcacca acgccccagc cagtgagtaa cacctcctga ccggcatgaa
     6121 aacgcggatc ttcgctggtg cgtacagttc cggcaaaatc gatcccagga atcatcggaa
     6181 aattacggat gatttttccc ttaccggtaa tcgccagcgc atctttatag ttcaggctcg
     6241 accagtgaac atcgaccatg acatcgccct ccggcaggcg actttcgtcc agagtctgta
     6301 ctgatgcgag agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata agtggtcctc
     6361 atgtgcatga tggattggaa aataatttct gaagattata ctcgctaatg gaaaagcaaa
     6421 gccgatgaag cgcaataaat tgcgagataa atctgatttg ctagtatgcc cgcttcctca
     6481 ctatcggagt taacacaagg atgagattaa cgacgaaatt ttcggccttt gttacgctgc
     6541 tcaccgggtt aacaattttt gtgactttgc tgggctgttc gctaagtttc tacaacgcaa
     6601 ttcagtataa gtttagtcat cgcgttcagg cggtggcgac ggcgattgat acccaccttg
     6661 tgtcgaatga cttcagcaca ttaaggccac aaattaccga attaatgatg tcggcagata
     6721 tcgttcgtgt agacctgctc catggtgata aacaggttta taccctggcc agaaatggta
     6781 gttatcgtcc ggttggctcc agcgatctgt ttcgtgaact gagcgttccg ttgataaagt
     6841 atccggggat gtcgctgcgt ctggtttatc aggatccgat gggcaactat ttccattcgt
     6901 tgatgaccac cgcgccgctc acgggggcga ttagctttat cattgttatg ctcttcttgg
     6961 cggtacgctg gttacaacgg caacttgccg ggcaagaatt gctggaaacc cgggctactc
     7021 gtatcttaaa cggtgagcgt ggctctaatg tgttgggaac catctatgaa tggccgccca
     7081 gaaccagcag tgcgctggat acgctgcttc gtgaaattca gaacgcacgc gaacaacaca
     7141 gccgtcttga tacgctgatc cgctcttatg ccgcccagga cgtgaaaacc ggcctcaata
     7201 accgactctt tttcgataat cagttagcaa cgttactgga agatcaggag aaagtaggta
     7261 cccacgggat cgtgatgatg attcgtctgc cggatttcaa tatgttgagc gatacttggg
     7321 ggcacagcca ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg acatttatga
     7381 tgcgctaccc tggcgcactg ctggcgcgtt accaccgcag tgattttgct gcgctgttac
     7441 cgcaccggac gttaaaagag gcagagagca tcgccggtca gttaatcaaa gccgtcgata
     7501 ccttgccgaa caataaaatg ctcgatcgcg acgatatgat ccacattggt atctgcgcct
     7561 ggcgtagtgg tcaggatacc gagcaggtaa tggaacatgc agagtctgcc acgcgtaatg
     7621 cgggattgca gggcggcaat agctgggcta tttacgatga ctcgttgcct gaaaaaggac
     7681 gcggtaatgt tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc ggcccgcgcc
     7741 tttatcaaaa accggcggtt actcgcgaag gtcaggttca tcatcgcgaa ctcatgtgcc
     7801 gcatcttcga tggtaatgaa gaggttagct cggcggagta tatgccgatg gtcttgcagt
     7861 ttggcttatc ggaagagtat gaccgtctgc aaatcagccg tcttattcca ctattgcgtt
     7921 actggccaga ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt cgcccgcgtt
     7981 ttcagcgttg gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga aaacgcataa
     8041 ttattgaact tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa cctgttattc
     8101 gtttagtgaa tgctttaggg gtacgggtag ccgtcaacca ggctggtttg acgctggtaa
     8161 gcaccagttg gatcaaagaa cttaatgttg agttactcaa gctccatccg gggctggtca
     8221 gaaacattga gaagcgaacg gagaaccagc tgctggttca aagcctggtg gaagcctgct
     8281 ccgggaccag cacccaggtt tacgccaccg gtgtgcgttc gcgaagcgag tggcagaccc
     8341 tgattcagcg cggtgttaca ggcgggcaag gggatttttt cgcgtcctca cagccacttg
     8401 atactaacgt gaaaaaatat tcacaaagat actcggttta acctgccgtt taatccgttt
     8461 tcacgtagaa taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc gccagattgt
     8521 tgcagcacat atgcagatga atgaccttac gcggttgcaa acaggcgagg aatgctgctg
     8581 atgcattaag cctttctgga ctcaggcaga gatttgtaac aaaggaaacg aactgcacta
     8641 attttcaccg tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt tggtaaagta
     8701 agcggatttt cttttccgcc ccagctttca ggattatccc ttagtatgtt gaaaaaattt
     8761 cgtggcatgt tttccaatga cttgtccatt gacctgggta ctgcgaatac cctcatttat
     8821 gtaaaaggac aaggcatcgt attgaatgag ccttccgtgg tggccattcg tcaggatcgt
     8881 gccggttcac cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat gctgggccgt
     8941 acgccgggca atattgctgc cattcgccca atgaaagacg gcgttatcgc cgacttcttc
     9001 gtgactgaaa aaatgctcca gcacttcatc aaacaagtgc acagcaacaa ctttatgcgt
     9061 ccaagcccgc gcgttctggt ttgtgtaccg gttggcgcga cccaggttga acgccgcgca
     9121 attcgtgaat ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga agaaccgatg
     9181 gctgccgcaa ttggtgctgg cctgccggtt tctgaagcga ccggttctat ggtggttgat
     9241 atcggtggtg gtaccactga agttgctgtt atctccttga acggcgtggt ttactcctct
     9301 tccgtgcgca ttggtggtga tcgtttcgac gaagctatca tcaactatgt gcgtcgtaat
     9361 tacggttctc tgatcggtga agccaccgca gaacgtatca agcacgaaat cggttcggct
     9421 tacccgggcg atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc agaaggtgtt
     9481 ccacgcggtt ttaccctgaa ctccaacgaa atcctcgaag cactgcagga accgctgacc
     9541 ggtattgtga gcgcggtaat ggttgcactg gaacagtgcc cgccggaact ggcttccgac
     9601 atctccgagc gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa ccttgaccgt
     9661 ttgttaatgg aagaaaccgg cattccagtc gttgttgctg aagacccgct gacctgtgtg
     9721 gcgcgcggtg gcggcaaagc gctggaaatg atcgacatgc acggcggcga cctgttcagc
     9781 gaagagtaat cggatgcagg caggggaaat gtctgtttac cctgcctagt ctgatacgag
     9841 aatacgcata acttatgaag ccaattttta gccgtggccc gtcgctacag attcgcctta
     9901 ttctggcggt gctggtggcg ctcggcatta ttattgccga cagccgcctg gggacgttca
     9961 gtcaaatccg tacttatatg gataccgccg tcagtccttt ctactttgtt tccaatgctc
    10021 ctcgtgaatt gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa ctagaacttg
    10081 aaaaccgggc gttacgtcag gaactgttgc tgaaaaacag tgaactgctg atgcttggac
    10141 aatacaaaca ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg cgtcaggatg
    10201 agcagaaaat ggtgactcag gttatctcca cggttaacga tccttatagc gatcaagttg
    10261 ttatcgataa aggtagcgtt aatggcgttt atgaaggcca gccggtcatc agcgacaaag
    10321 gtgttgttgg tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg ctgatttgtg
    10381 atgcgaccca cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta attgcagccg
    10441 gtaacggttg tacagatgat ttgcagcttg agcatctgcc agcgaatacg gatattcgtg
    10501 ttggtgatgt gctggtgacc tccggtctgg gcggtcgttt cccggaaggc tatccggtcg
    10561 cggttgtctc ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt caggcgcgtc
    10621 cgactgcagg actgcaacgt ttgcgttatc tgctgctgct gtggggcgcg gatcgtaacg
    10681 gcgctaaccc gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt ctgatgcaga
    10741 tgatgccgca ggtattgcct tcgccagacg cgatggggcc aaagttacct gaaccgacaa
    10801 cggggatcgc tcagccgact ccgcagcaac cgacgacagg aaatgcagct actgcgcctg
    10861 ctgctaatcg ctctccacaa agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc
    10921 gtgcgccggg agggcaatag tggcgagcta tcgtagccag ggacgttggg taatctggct
    10981 ctctttcctc attgcgctgt tgctgcaaat catgccctgg ccggataacc tgattgtttt
    11041 ccggccaaac tgggtgttac ttatcttgtt gtattggatc ctggccttgc cccatcgcgt
    11101 aaatgtgggc acaggttttg tgatgggtgc catactggat ctgatcagcg gctcgacgct
    11161 tggcgtacgc gtattggcga tgagcatcat tgcttacctg gtggcgctga aataccagct
    11221 tttccgcaac ctcgcattat ggcagcaggc gctagtcgtc atgttgcttt cgctggtggt
    11281 ggatattatt gttttctggg cagagttttt agtgattaac gtctctttca gaccggaagt
    11341 gttctggagt agtgtagtca atggggtgct ctggccgtgg attttcttgc tgatgcgtaa
    11401 agtccgtcag cagtttgcag tgcaataaag gtttctatga cttctctgta tttagcttcc
    11461 ggttctccgc gtcgtcagga gttacttgcg caacttggcg tgacctttga acgtattgtt
    11521 acgggcattg aggagcagcg tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg
    11581 gcacacaaga aagcacaggc gggtgtcgcg caaacggcgc aggatctccc ggtgctgggt
    11641 gcggatacta tcgttatcct gaatggagaa gtgctggaga aaccgcgcga cgcagagcat
    11701 gcggcgcaga tgttgcgcaa attatcgggt cagacccatc aggtgatgac ggcagtggtg
    11761 ttggccgaca gccagcacat tctcgattgc ctggtggtca ccgatgtgac tttcagaacg
    11821 ttaacagacg aagacatcgc gggctatgtc gccagcggtg aaccgttaga taaagcaggt
    11881 gcatacggta ttcaggggct gggtggctgt tttgtcagga agataaatgg cagctatcac
    11941 gccgtagtcg gcttaccgct ggttgaaacg tatgaattat taagtaattt taacgcactg
    12001 cgtgagaaaa gggataaaca tgacggctga attgttagta aacgtaacgc cttcggaaac
    12061 gcgagtggcg tatattgatg gcggtattct gcaggaaatt catattgaac gtgaggcgcg
    12121 acgcggaata gtaggcaata tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca
    12181 ggcggctttt gtagatattg ggctggataa agccgcgttt cttcatgcat ccgatatcat
    12241 gccgcacacc gaatgtgtgg cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc
    12301 ggaactggtt cgtcaggggc aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac
    12361 taaaggtgcg cgcctgacca ccgatatcac gctgccttct cgctatctgg tgtttatgcc
    12421 aggggcttct cacgttgggg tttcccaacg tattgaaagc gaatcagaac gtgaacgcct
    12481 gaaaaaagtg gtcgcagagt attgcgacga gcagggcggg tttatcatcc gtaccgcagc
    12541 ggaaggggtt ggcgaggctg aactggcctc cgatgccgct tatctgaaac gcgtctggac
    12601 caaagttatg gagcgtaaaa aacgcccgca gacccgttat cagctgtacg gcgaactggc
    12661 gctggcgcag cgtgttctgc gtgatttcgc cgacgccgaa ctggaccgca ttcgcgttga
    12721 ctcacgcctg acttacgaag cattgctgga gtttacctcg gagtacattc ccgagatgac
    12781 cagcaagctg gagcattaca ccggacgcca gccgattttc gatctctttg atgtcgaaaa
    12841 cgaaatccag cgagcgctgg aacgcaaagt agaactgaaa tccggtggtt atctgattat
    12901 cgaccagacc gaagcgatga ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg
    12961 caatctggac gacaccattt tcaataccaa tattgaagcg acgcaggcta tcgctcgcca
    13021 gttacggttg cgtaatctgg gtgggattat cattattgat ttcatcgata tgaataatga
    13081 agatcaccgc cgccgcgtgc tgcactcgct ggagcaggcg ttgagcaaag accgggtgaa
    13141 aaccagcgtt aatggttttt cggcgctggg gctggtggag atgacgcgta aacgcacccg
    13201 cgaaagcatt gagcacgtac tgtgtaacga atgcccaacc tgccacggtc gcggaacggt
    13261 gaaaaccgtg gaaacggtat gctatgaaat catgcgcgag attgttcgtg tccaccatgc
    13321 ttacgactcc gaccgtttcc tggtctatgc ttctccggca gtagctgaag ccttgaaagg
    13381 cgaagagtca cactcgctgg cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca
    13441 aattgaaccg ctctataacc aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg
    13501 gccgccatcc ggcaaagggt ttttgagtca catttttagc agacaaggag tgacgggtga
    13561 ggcgattgcc ggggatttta ctgcttactg gagccgcgct cgttgtgatc gcagccctgc
    13621 tggttagcgg cctgcgtatt gctttaccgc atcttgacgc ctggcgtccg gaaatcctca
    13681 acaaaataga atccgcgact ggcatgccgg tagaagccag tcagctctca gccagctggc
    13741 agaattttgg cccgacgctt gaagcacacg acatccgtgc agaactaaaa gatggcggcg
    13801 aattttcggt taaacgcgtt actctggcgc tggatgtctg gcagagcttg ttacatatgc
    13861 gctggcagtt tcgcgacctc actttctggc agctgcgctt tcgcaccaac actcctatca
    13921 ccagcggtgg tggtaatgat agcctggaag ccagtcacat cagcgatctg tttcttcgtc
    13981 aatttgacca tttcgatctc cgcgacagtg aagtcagttt tctgacgcca tccggtcagc
    14041 gcgccgagct ggcgatccca caactcacct ggctgaacga tccacgtcga caccgtgcgg
    14101 aaggcctggt aagcctctcc agccttaccg gacagcacgg cgtgatgcag gtgcgcatgg
    14161 atttgcgcga tgatgagggg ttgttaagca atggtcgcgt ctggctacag gcggatgaca
    14221 tcgacctgaa gccgtggctc ggtaaatgga tgcaggacaa tattgctctg gaaacggcac
    14281 agttctccct tgaaggctgg atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct
    14341 ggctgaaaca gggcggtgcc agctggttgg gcgagaagca aacgcatacg ctgtcggtgg
    14401 ataatctgac cgcgcatatt acgcgtgaaa atccgggctg gcagttctct attcccgata
    14461 cacggatcac gatggacggc aaaccctggc cgagcggagc attgacgctg gcctggatac
    14521 cggaacagga cgttggcggc aaagacaata aacgcagtga cgaactccgg attcgcgcca
    14581 gtaatctgga gctggcaggc ctggagggca tacgcccgct ggccgcgaaa ctttcacctg
    14641 cactgggtga tgtttggcgc tccacacaac cgagcggcaa gattaacact ctggcgctgg
    14701 atatcccgct tcaggcggca gacaagaccc gttttcaggc atcgtggagc gatctggcct
    14761 ggaagcaatg gaaattatta ccgggtgcgg aacacttctc cgggacgctt tccggcagcg
    14821 ttgaaaatgg tttgcttacc gcgtcgatga agcaggcaaa gatgccttac gaaacggtat
    14881 tccgtgcgcc actggaaatc gccgacggcc aggcaactat aagctggctg aacaatgaca
    14941 aaggtttcca cctggatggg cgtaatattg acgttaaagc caaagctgtc catgcgcgcg
    15001 gcggttttcg ttacctgcaa cctgctaacg atgaaccctg gctgggtatt ctggctggca
    15061 tcagtaccga tgatggttca caagcctggc gctatttccc ggaaaacttg atgggtaaag
    15121 acctagttga ttacttaagt ggcgcgattc agggcggtga agcggataac gcgacgctgg
    15181 tttatggtgg caatccgcaa ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc
    15241 tggtgccgct gcgtaacgcg aagtttgcct tccagccgga ctggcctgca ttaactaacc
    15301 ttgatattga actggacttt attaacgacg gtttatggat gaaaaccgat gacgttaatc
    15361 tgggcggcgt gcgcgcgagt aatctcaccg cagtgatccc tgactactcg aaagaaaaac
    15421 tgctgattga cgctgacatt aaaggtccgg gtaaagccgt tggcccttac tttgatgaga
    15481 caccgctgaa agattctctg ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga
    15541 atgctcgctt acatcttgat atcccgctga acggcgaact ggtaaccgcg aaaggtgaag
    15601 tgacgctgcg taataacagt ctgtttatca aactactcga cagcaccctg aaaaatttga
    15661 gcggtaaatt cagctttatc aatggcgatc tgcaaagtga accactgaca gcaagctggt
    15721 ttaatcagcc gttgaacgtg gatttttcca ccaaagaagg ggcaaaagcc taccaggttg
    15781 cggtaaacct caacggtaac tggcaaccgg cgaaaaccgg cgttctgcct gcagcggtga
    15841 acgaagcatt gagtggcagc gtggcgtggg atggtaaagt gggcattgat ctgccttatc
    15901 atgctggcgc gacctataac gtagagctga acggcgatct gaataatgtg agcagtcact
    15961 taccttcacc gttagccaaa cctgcgggtg aaccactgcc ggtaaacgtt aaggttgatg
    16021 gcaatctcag cagctttgaa ttaaccggac aggctggtgc ggataaccat ttcaatagcc
    16081 gctggttgct tggtcaaaag ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa
    16141 cgctcccgcc gttgccggaa caaagtggcg ttgaactcaa tatgccgccg atgaatggtg
    16201 ccgagtggct ggccctgttc cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt
    16261 tcccacaaca cataacgtta cgtacgccta tgttgtcact gggaaatcag caatggaata
    16321 acctgagtat tgtttcgcaa ccgacggcaa atggcaccct ggttgaggcg caggggcgtg
    16381 aaatcaatgc cacgctggcg atgcgtaata acgcgccgtg gctggcgaat atcaaatatc
    16441 tttattacaa cccgagcgtg gcgaaaactc gtggtgattc aacaccgtca tcacctttcc
    16501 cgacaacgga gcgcattaac ttccgtggct ggccggacgc acaaatacga tgcacagagt
    16561 gctggttctg ggggcaaaaa ttcggtcgta ttgacagtga tctcaccatt tctggcgata
    16621 cgttaacgct gaccaatgga ctgattgata ctggtttctc gcggcttact gccgatggtg
    16681 aatgggttaa taatccgggg aatgaacgta cctcgctgaa aggaaaactg cgcgggcaga
    16741 aaattgatgc cgccgcagaa ttttttggtg tcacgacgcc catacgccag tcgtcattta
    16801 atgtggatta cgatttacac tggcgcaaag caccctggca accagatgag gcgacgttga
    16861 atggcatcat tcatactcaa ctgggtaaag gcgaaattac cgaaatcaat accggacatg
    16921 ccgggcaatt gctgcgctta ttgagcgttg atgccctgat gcgtaagctg cgttttgatt
    16981 tcagagacac ttttggcgaa gggttctatt ttgactccat tcgcagcacc gcgtggatta
    17041 aagacggcgt tatgcacacc gacgacacgc tggtggatgg cctggaggcg gatatcgcca
    17101 tgaaagggtc ggtaaatctg gtacgtcgcg acctgaatat ggaagcggtt gtcgcaccag
    17161 agatttctgc gacggtgggc gtggctgcgg cttttgcggt taaccccatt gttggcgcgg
    17221 cagtatttgc agccagtaaa gtgctggggc cgctgtggag caaagtctcc attttgcgct
    17281 atcacatttc gggtccgctg gacgatccgc aaatcaacga agtgttgcgc caaccgcgta
    17341 aagaaaaagc gcaatgattt gacgagggcg cgtaattgcc ccaatctcat atgataatcg
    17401 ttgccaaagg ccaacgagcc agaacataac cgtaggtcgg atagggcgtt cacgccgcat
    17461 ccggcagccg taaaaaatcc tctactgcag taactaacga gtagcaaaaa cgatgagtct
    17521 taacctagta agtgaacaat tgctagcggc gaacggcctg aaacatcagg acttgttcgc
    17581 gatcctcggt caactggccg aacgtcgcct tgattatggc gatctctatt ttcagtcgag
    17641 ctatcacgaa tcctgggttt tagaagaccg cattattaaa gatggttctt acaacatcga
    17701 tcagggcgtt ggcgtgcgtg caatcagcgg tgaaaaaacc ggatttgctt acgctgacca
    17761 aatcagcctg ctggcgctgg aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag
    17821 tggtgatggc aaagtacaga cgctgggcgc ggtagggcat agcccgttgt atacctcggt
    17881 agatccgctg caaagcatga gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa
    17941 ggttgctcgc gaagcggaca agcgcgtaca ggaagtgact gccagcctca gtggtgtcta
    18001 tgaattaatt ctggttgcgg ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt
    18061 gcgtctttcc gtgagcgttc tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg
    18121 cggtggcggt cgttttggtt atgagttctt ccttgccgat cttgacggcg aagtccgtgc
    18181 ggatgcatgg gcaaaagaag cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc
    18241 accagcgggc accatgccgg tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga
    18301 agcggtaggt cacggtctgg aaggcgactt caaccgccgc ggcacttcag tatttagtgg
    18361 gcaggtcggg gagctggtgg cttcagaact gtgtaccgtg gttgatgacg gcacgatggt
    18421 cgatcgccgt ggttcggtgg cgattgatga cgaaggtacg ccaggccagt acaacgtgct
    18481 gattgagaac ggcattctga aaggctacat gcaggataaa ctcaacgcgc gtttgatggg
    18541 gatgacgccg actggcaacg gtcgccgtga atcctacgcc catctgccca tgccgcgtat
    18601 gaccaacacc tatatgctgc cgggtaaatc gaccccgcag gaaattattg aatccgttga
    18661 gtacggtatc tatgcaccga actttggtgg cggtcaggtg gatatcacct ccggcaaatt
    18721 cgttttctcc acttcagaag catatctgat tgaaaacggt aaagtaacga agccggtgag
    18781 aggcgcaacg ttgattggtt ccggtatcga aaccatgcag cagatttcga tggttggcaa
    18841 cgacctgaaa ctggataacg gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt
    18901 tggcgtgggc cagccaacgc tgaaagttga taacctgact gttggcggta ctgcgtaata
    18961 atttcattat tttcagaagg ataattaatc tttctacgtg cacgaacggt cccctcgctc
    19021 ctctggggtt agggtgaggg gaacccgttg gcacaggttt gtagaacgta acagtacaat
    19081 atgaattact tctctttccc gcgcccgtgc atctcctgaa acaatttacc gacctcaaca
    19141 aaataatccg tcagcgagtt gatcacgacc tgtaccttca gcggcagctt atctttttcg
    19201 gtatataacg cataaaccgg gcgtggatct gactgataac gcggcagcag gatctccagc
    19261 tccccacgat tgatctcgtt gatcacccac atcagcggca cgtaggcgat cccggcaccc
    19321 gccgttagcc agcgcaccag cgtcatcgga tcattagtca caaatctccc ttgcgggatc
    19381 aggcgagtcg agatcccttc cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg
    19441 tattcaagcc atgaatggct acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg
    19501 agatagcttt tcgcggcgca caccaccatt ggcatcgcgc caagacggcg ggaaaacagg
    19561 ctggaatcct gcaacgcgcc gacgcggatc accacatcca gaccgtcggc aatcaggtcg
    19621 ggggctggaa ttccggtaac cagattgacg ctcaaacctg ggtattcttt cagcattttg
    19681 gctgtcagcc cggcgagaac attttgtgcc atagttgaag aacagccaat gcgtagcgtc
    19741 ccgatggggg tgttattgaa agcatacagt tgctcatgaa catcctgcac ttcatgaagc
    19801 atacgacggc agccctggta gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg
    19861 ctacggttta acagctttac ctgcaactca tcttccagtt ttgacaccgt ctgactgatg
    19921 gacgaaacgc tcatctgtag ctgtctggcg gcggcggtaa aagagccaaa ttcaactact
    19981 ttggcaaaca ccgacatgcg ttttagtcgt tccattattc actctgactt aaaagtgatt
    20041 tagatcacat aatat
//
LOCUS       DAJTYD010000060        19904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTYD010000060 DAJTYD010000000
VERSION     DAJTYD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.060"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..182)
                     /gene="nudI"
                     /locus_tag="OO585_001955"
     CDS             complement(<1..182)
                     /gene="nudI"
                     /locus_tag="OO585_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6629557.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGE"
     gene            461..1003
                     /locus_tag="OO585_001956"
     CDS             461..1003
                     /locus_tag="OO585_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629558.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARSTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1103..2305
                     /locus_tag="OO585_001957"
                     /pseudo
     CDS             1103..2305
                     /locus_tag="OO585_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2525..3307
                     /locus_tag="OO585_001958"
     CDS             2525..3307
                     /locus_tag="OO585_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6629559.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3322..4527
                     /gene="rhmD"
                     /locus_tag="OO585_001959"
     CDS             3322..4527
                     /gene="rhmD"
                     /locus_tag="OO585_001959"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCR6629560.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4584..5873
                     /locus_tag="OO585_001960"
     CDS             4584..5873
                     /locus_tag="OO585_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6629561.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5891..6694
                     /gene="yfaU"
                     /locus_tag="OO585_001961"
     CDS             5891..6694
                     /gene="yfaU"
                     /locus_tag="OO585_001961"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6629562.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6735..7637)
                     /locus_tag="OO585_001962"
     CDS             complement(6735..7637)
                     /locus_tag="OO585_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6629563.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7830..9020)
                     /gene="glpC"
                     /locus_tag="OO585_001963"
     CDS             complement(7830..9020)
                     /gene="glpC"
                     /locus_tag="OO585_001963"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6629564.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9017..10276)
                     /gene="glpB"
                     /locus_tag="OO585_001964"
     CDS             complement(9017..10276)
                     /gene="glpB"
                     /locus_tag="OO585_001964"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6629565.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10266..11894)
                     /gene="glpA"
                     /locus_tag="OO585_001965"
     CDS             complement(10266..11894)
                     /gene="glpA"
                     /locus_tag="OO585_001965"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6629566.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12167..13525
                     /gene="glpT"
                     /locus_tag="OO585_001966"
     CDS             12167..13525
                     /gene="glpT"
                     /locus_tag="OO585_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6629567.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13530..14606
                     /gene="glpQ"
                     /locus_tag="OO585_001967"
     CDS             13530..14606
                     /gene="glpQ"
                     /locus_tag="OO585_001967"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6629568.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14648..14890)
                     /locus_tag="OO585_001968"
                     /pseudo
     CDS             complement(14648..14890)
                     /locus_tag="OO585_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15069..15719
                     /gene="inaA"
                     /locus_tag="OO585_001969"
                     /pseudo
     CDS             15069..15719
                     /gene="inaA"
                     /locus_tag="OO585_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(15773..16027)
                     /gene="yfaE"
                     /locus_tag="OO585_001970"
     CDS             complement(15773..16027)
                     /gene="yfaE"
                     /locus_tag="OO585_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6629569.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16027..17157)
                     /gene="nrdB"
                     /locus_tag="OO585_001971"
     CDS             complement(16027..17157)
                     /gene="nrdB"
                     /locus_tag="OO585_001971"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6629570.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17304..19589)
                     /gene="nrdA"
                     /locus_tag="OO585_001972"
     CDS             complement(17304..19589)
                     /gene="nrdA"
                     /locus_tag="OO585_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6629571.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4624 a         4870 c         5407 g         5003 t
ORIGIN      
        1 tgttctccca gttcttcgcg aatttcgcgg cgtagtgcct cttcaattcg ttcgccatac
       61 tccacgccgc cacccgaaag cgcccattga ccggggaaaa cgccgcgatc gtcggccatt
      121 ttgcacagca gataagcacc atcattttga attaaagggc atacaatagt ccgttgtcgc
      181 acaggggtat tccttataaa taaaaagaaa ccatgagaat acggtaactc agaaatatgt
      241 gaaagtgaca gtaatctctt ttgcgatgat aactgcactt attattattt ttgtgtgtat
      301 cggtaaagtg tgtttatttt ttcggcgttt aattatttaa gaagtattta gttacaataa
      361 gtgactagat cccttttttc gctcattgaa tcctctgtgc caggcagtac aatcccgcag
      421 ctttgggaat tttgattaat tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc
      481 aggtctggcc ggaatgctgt tggtttctgc atcggtcaat gcaatgagca tcagcggtca
      541 ggcgggtaaa gaatacacca atattggtgt cggttttggg actgaatcga cgggcctggc
      601 gttaagcggt aactggacac ataacgacga cgacggtgac gtcgctggcg tggggctggg
      661 gttgaatctg cctctagggc cgttaatggc gaccgttggc ggaaaaggcg tttacaccaa
      721 cccgaattac ggcgatgaag gttatgccgc agcggtagga ggtggtttgc agtggaaaat
      781 tggcaacagc ttccgtttgt ttggcgagta ttactactct ccggattcac tctccagcgg
      841 tattcaaagt tatgaggaag cgaatgctgg cgcgcgttcc accattatgc gtccagtcag
      901 tattgaggcg ggttatcgct acctgaattt gtcgggtaaa gatggtaacc gcgacaacgc
      961 cgtggctgac ggcccgtatg ttggggttaa cgccagtttc tgatcccttc cggcgcggca
     1021 ttctgtcgcg ccattcagtt tcttactacg ccttatctcc ctttgcgtta tagtgattcg
     1081 ataacaaaag caggagataa ccatgttaaa agtggaaatg ttatccaccg gggatgaagt
     1141 gttacacggg caaatcgttg acactaacgc tgcctggctg gccgattttt tctttcatca
     1201 ggggctgcca ttatctcgcc tcaatacggt gggggataac cttgatgact tagtcaccat
     1261 tcttcgcgaa cgtagtcagc acgccgatgt gctgatcgtt aacggcgggc tgggaccgac
     1321 cagcgatgat ttaagcgcac tcgccgctgc gacagcaaaa ggtgaaggcc tggtgctgca
     1381 tgaagcctgg ctcaaagaga tggaacgcta tttccacgaa cgtggacgag taatggcacc
     1441 gagcaaccgt aaacaagcgg agctgcctgc cagtgctgaa tttatcaata acccggtagg
     1501 caccgcctgt ggttttgccg tgcagcttaa tcgttgcctg atgttcttta ctcccggcgt
     1561 accgtcagaa tttaaggtga tggtcgagca cgaaatcctg ccgcgcctgc gcgagcgttt
     1621 ttctttacca cagccgccgg tttgtctgcg tttaactacc tttggtcgtt cggaaagcga
     1681 tctggcacaa agcctggata ctctacaact gccgccgggc gtaacaatgg gctatcgctc
     1741 ttcaatgccg attatcgaac tgaaactcac cggaccggca agcgagcaac aggcgatgga
     1801 aaaactgtgg ctggacgtta aacgtgttgc cggacagagc gtgattttcg aaggcaccga
     1861 aggactgccc gtgcagatca gtcgcgaatt gcaaaaccgc cagttcagcc tgacgttgag
     1921 cgagtaattc accggtggtt tattggcttt gcaactttct cgcgcaggtg ctccattgct
     1981 ggcgtgtgaa gtggttcctt cacaggaaga aaacctggcg caaactgcgc actggattac
     2041 agaacggcgg gccaaccatt ttgccgggct ggcactggct gtttcgggtt tcgagaacga
     2101 acatctcaac tttgcgctag ccacaccaga cggcactttc gctctgcgtg tgcgtttcag
     2161 cactacgcgc tacagcctgg ctatccgtca ggaagtgtgc gcaatgatgg cactgaatat
     2221 gctgcgccgt tggttaaacg gccaggatat cgccagtgag catggctgga ttgaggttat
     2281 tgagtccatg accttatctg tctgattccg ccactctgtg ccggtttact gcaccggcac
     2341 attccccctc cggcaaaact tgtccaacct gttgcactca ataagaaaaa atccgtgcac
     2401 tgattcacaa atttgtgcgt aatttctcca gtccgctgga cgaggagcgg gatttgatgc
     2461 gcttcacaat tttactcccc ctgagcaacg agactgattc cagtatctaa gactggagtc
     2521 cagtatgttg gaatcaagca aagttcccgc cctgacacgg gctattgata ttctcaattt
     2581 gattgcccgc attggcccct gtagtgctgc gacgatcatt gacacattgg gaatccctaa
     2641 aagcacggcc tatttgctgc ttaatgaact caggcgtcag cgttttctta gccttgatca
     2701 ccaggaaaat ttttgtttgt ggaccaggct ggtggagctt tctggtcatg cgttaagcaa
     2761 gatggatctc cgggaactgg ctcgaccgcg tttgacgcaa ctgatggata ccaccgggct
     2821 gttatgtcat ctggggatta ttgataacgg cagcgcttat tacattttaa aagtggagtc
     2881 atcagccact atcagcgtgc gttcccatga aggaaaaagc ctttcgcttt atcgctccgg
     2941 aataggtaaa tgcctgcttt cctggcaacc tgcagcggta cagcaaagta ttatcgaagg
     3001 gctggtctgg gagcaggcga cacccacaac gattactcat ccacagcaac ttcacgaaga
     3061 actggcgcgt attcgccgtc agggctggag ctatgacaac ggcgaagatt accccgatgt
     3121 ccgttgtgtc gctgcgccgg tgtttaacgc caataacgag cttaccgctg cgatctcggt
     3181 ggtcggtacc cgcttacaaa tcaatgaaga ataccgtgat tatctggcgg gcaagtccat
     3241 tgcctgcgcc agggatattt cacgtctgtt gggatggaaa agtccctttg acttacaagc
     3301 ctcataacaa tggagaacat catgacccta ccaaaaatta aacaggttcg cgcctggttt
     3361 actggcggtg cgacagcaga aaaaggcgct ggcggcggtg attatcacga ccagggggcg
     3421 aatcactgga tagacgatca tattgccacc ccgatgagta aataccgcga ttacgagcag
     3481 tcacgccagt catttggcat taacgttctt ggcacgttgg ttgttgaagt cgaagcagaa
     3541 aacggccaga ccggattcgc tgtttcgaca gccggtgaaa tgggctgttt tatcgtcgaa
     3601 aaacacctta accgtttcat tgagggtaaa tgtgtcagtg atatcaaact gatccacgat
     3661 caaatgctca atgccaccct gtattactcc ggctctggtg gcttggtgat gaatacgatt
     3721 tcctgtgtcg atctggctct gtgggatctg ttcggcaaag tggtcgggct tccggtttat
     3781 aaacttttag gcggcgctgt tcgtgatgag attcagttct acgccacagg tgcgcgtccg
     3841 gatctggcaa aagagatggg ctttatcggt ggcaaaatgc cgacgcactg ggggccacat
     3901 gatggcgatg cggggatccg caaagatgcc gctatggtcg cggatatgcg tgaaaaatgc
     3961 ggtgaagatt tctggttaat gctcgactgc tggatgagtc aggacgtgaa ctatgcgacc
     4021 aaactagccc acgcttgcgc gccctataac ctgaaatgga tcgaagagtg cctgccgcca
     4081 cagcagtatg aaggttatcg cgaactgaaa cgcaacgcgc cagtcggaat gatggtcacc
     4141 agcggtgagc accacggcac actgcaatct tttcgtacgc tttcagaaac cggtatcgac
     4201 attatgcagc cggatgttgg ctggtgcggt ggtttaacca cgctggtgga aattgccgca
     4261 atcgccaaat ccagggggca actggtggtg ccgcacggtt cgtctgttta ctctcaccat
     4321 gcggtgatca ccttcaccaa tacgccattc agcgaattcc tgatgaccag cccggattgt
     4381 tcaacgatgc gtccgcagtt tgacccgatt ctgctcaatg agccggttcc ggtgaatggt
     4441 cgtattcata aatcagtgct tgataaaccc ggttttggcg tcgaactcaa tcgtgactgc
     4501 aatttgaaac gcccctacag ccactaatca ctccgggcgt tgctgtgaca acggtaacgc
     4561 ccacatattc taaggagctt tttatgagca ccgctttgct tgacgccgtg gtgaagaaaa
     4621 accgtgtgcg tttaattccg tttatgttgg cgctgtatgt gctggcgttt ctcgaccgtt
     4681 cgaatatcgg ttttgccaaa cagacctacc agattgatac cgggttgagt aatgaagctt
     4741 atgcgctggg agcaggcatt ttctttgtgg tatatgcgtt tctgggtgtt ccggcgaatc
     4801 ttttgatgcg caaactgggg gccagaacct ggattggtac gacaacactg ctgtggggat
     4861 ttctttcggc agccatggca tgggccgata ctgaagcgaa atttctgata gttcgcactc
     4921 tgcttggtgc tgcggaggcc ggatttttcc ctggtatgat ttatctcacc tcgcaatggt
     4981 ttccgcagcg taatcgcgcc agcattatgg ggctgttcta tatgggcgca ccgctggcgt
     5041 taacactggg atcaccgctt tccggcgcgc tgttggagat gcatggattt atggggcatc
     5101 ccggctggtt ctggatgttt gtgattgaag gattgttggc agtcggtgct ggggtattca
     5161 cattcttttg gcttgatgac acaccggagc aggcacgttt tctgagtaaa caagaaaaaa
     5221 cgttgcttat caatcaactg gcaagtgaag aacaacagaa agtgacttct cggctgagcg
     5281 atgcgctgcg taatggccga gtctggcaac tagcgattat ctacctgacc attcaggtgg
     5341 cggtttacgg attaattttc ttcctgccga cccaggttgc ggcattgctg ggaacaaaag
     5401 tgggctttac agcgtcggtg gtcaccgcca ttccgtgggt tgcggccttg tttgggacct
     5461 ggcttattcc gcgctattcc gataaaaccg gcgaacggcg taatgtcgca gcgctgacat
     5521 tactggcggc gggcattggt attggtctgt ccgggctgct ttctccagta atggcgatcg
     5581 tagcgctgtg tgttgcagct atcgggttta ttgccgtgca gccggtgttc tggacgatgc
     5641 cgacacaact gctttccggt acggcgctgg ctgcggggat tggttttgta aacctgtttg
     5701 gtgcagtggg cgggtttatt gccccgatcc tgcgcgtgaa agcagaaacg ttatttgcca
     5761 gcgatgcggc aggattactg acactggcag cggtggcggt catcggttcg ctgattattt
     5821 tcactctgcg tgtaaatcgt actgttgcgc agaccgacgt ggcacatcat taaataggtt
     5881 aaggaacacg atgaacgcat tattaaccaa tccctttaaa gaacgtttac gcaagggcga
     5941 agtgcaaatt ggtctgtggt taagctcgac tacttcttat atggcagaaa ttgccgctac
     6001 ttccggttat gactggttgc tgattgacgg ggagcacgcg ccaaacacca ttcaggatct
     6061 ttatcaccag ctacaggcgg tagcgcccta tgccagccaa cccgtgatcc gtccggtgga
     6121 aggcagtaaa tcgctgatta aacaagttct ggatattggc gcgcaaactc tattgatccc
     6181 gatggtcgat actgccgatc aggctcgtca ggtggcgtct gccacgcgct atcctcccta
     6241 cggtgagcgt ggtgttgggg ccagtgtggc acgggctgcg cgctggggac gcattgagaa
     6301 ttacatggcg caagtcaacg attcgctttg tctgttagtg caggtggaaa gtaaaacggc
     6361 actggataac ctggacgaga tcctcgacgt cgaagggatt gatggcgtgt ttattggacc
     6421 agcggatctt tctgcgtcgt tgggctaccc ggataacgcc gggcacccgg aagtgcagcg
     6481 aattattgaa accagcattc ggcggatccg tgctgcgggt aaagcggctg gttttctggc
     6541 tgtggctcct gatatggcgc agcaatgcct ggcgtgggga gcgaactttg tcgctgttgg
     6601 cgttgacacg atgctctaca gcgatgccct ggatcaacga ctggcgatgt ttaaatcagg
     6661 caaaaatggg ccacgcataa aaggtagtta ttgatatcaa aggcccatgg ggatcggctg
     6721 tgggcctgtg ttaattagtg gttattcgct gccagatcgg cttcgcttag ctgggtggcc
     6781 gcgagcacct ggtcacgatc aatgccctgt tccagcatgc gcaaagcaat tttaatggct
     6841 tgttcatgca taccttcctg ccagccaatt tgatgccctt cctgccgtag ccgttcagca
     6901 atagtcatta atctctcctt ttgtagtggt gaacgttcgg caatctcctc aataaaacgg
     6961 gtgaaacggg aggtatcgcc gcattgcagc agataattaa acagtgtttg tagctggctg
     7021 tcattagtga agcctctaac caaaagcgtg gtgatcctgt cgaccatgcc gattaaatcg
     7081 cggtcgcgaa tatgcttttg aatcagttcc agcagagcta tacgccgatg ttgcatgatc
     7141 tcatcgtcag gtacgatggt gatatccacc aacggaaaag cttcggtgta caactgccgg
     7201 gcaagttgcg gatcgtcaaa ctcatccagc cagttaagtg agtacgggta gggcgatgtt
     7261 tcgccatgat aaaacagcaa tggcaccacc agcggaactc tgtcatagcc tttatccagg
     7321 tgacgctgca tggcggcagt ggcatagcgc attagccgaa aagccatatt cttttctgca
     7381 gagctttgat gttcaatcac gcagtagata tagccgtcac cgtcgctggt ttccacggac
     7441 cacaaaacat ccgagtagta agcgcgtaaa cttttttcaa taaaactagt gggttccagg
     7501 cgtaaggttt gcaggttgca aagcttgcgc agtggttctg gtaaatgtat ttcgagaaaa
     7561 tcccgtgcgg tttcgggtgt gaacataaag gttttaaata ccgcatcatg cggggaggag
     7621 gttgttgatt cggtcatggt tgtccgtcac ctgatattta tgatgacgcg acattacggt
     7681 tatcctaggc ctttgacatc tgccagtttc agcctttgcg agacgttttc agaggaattt
     7741 tcactgcgtt gcaacgcata cattgggcac ggaatcgtgt acgcaaaaat gcccggtaac
     7801 cacatgctta ccgggcatca gaaaggagtt taagccagcg cctgggccag tagcgtaatc
     7861 ggatgttcgc agcgaagact tgtggacatc tcaatctgcc atttacaggt ttcgcagtcg
     7921 gtgaccacca gatctgcgcc gctttcttct atctggcgga acagtggtgc gccgatggct
     7981 tgtgaggtgg ggtagttctc ttttttgaaa ccgtaagtac ccgcaatacc gcagcactgg
     8041 gaatccagca ccgttaactc aagccccggg attttacgca acagctccag ggtgtagagc
     8101 gtccagccca ttttttccat atggcacgga gtgtgataaa ccactttcag cggcagcggt
     8161 ttcagcggta acgttttgcc ttcgtccagc ttgcgccaca gccagcgggt tgccagttcg
     8221 atatgatcgc gcaagccttt gttgtcgaca ttcagcactt ccgggtattc gtcgcgcagg
     8281 gcaaatgtac aggttgagga ggtggcaatc accggaatgc cttttactcc cacagcttcg
     8341 cggatcgact ctacattcgt aattgcctgt ttgcgtgctt tatcggtaaa gccgttggcg
     8401 attagcggta cgccgcagca tttttctttg ctgagcagtt gtacaccggt acccattgcg
     8461 ttgagcactt taattaaatc tttacctaac tgcggatggt tgtagttaac gaagcagccg
     8521 tgaaagaaag cgacctggtc tttatattgt gcttgctgag ccgctatgct gcgataccag
     8581 cgacggaacg tgccgaagga gtatttcggt agcgtgcgac gatgatcgat ttttaacgcc
     8641 gcatcaagca gctgccgcac cggtttcagc gaggtagcgg tgttgacgat tggtgcgaac
     8701 ggcgtggaaa cgctacccat caggtcggta tgactcaaca caaaattacg cagcgacggg
     8761 cgcgtggtgt catattttgc ccgcgcgcgc tggataatat cgccaatctt cacatcggac
     8821 gggcaggcga cttcacaacg tttgcagttg atgcaatatt tcagcgcctc gtcatacagt
     8881 gcgccatctt tcaaacgcag acgctcgcca tccggcccgg cttgttttgg ccctggataa
     8941 ccgggattca cccggctcac cgggcaggcg gtggtgcaga cggtgcactt aatgcagttt
     9001 tcgaagctgg tgtcgttcat tgttggcctc ctgcgcgttg ggcaatctgt tgagcggcat
     9061 gtaaagcact gacggcacaa acaccgccgc cgcatccctg ggcgatggga tcaaatccgc
     9121 ccagcaccga accgatggca aacaggttct caatggtttg ccctgcctgc gacgggcgta
     9181 gcgtctcatc agtggttaca ccgaactgct gccacggttg cggcgcaaaa aaatcgccct
     9241 tataccattc accccgcgtg gcggtttgta gcacatcaag gccgagaatc ggctcgcgaa
     9301 tgccgttacg ttccgctacc agtccgccac taaagaaact gccgctggcg agaaccgcga
     9361 aatgtggacg tagcggaata tcggcgtgat tgcgggtcca gatttcattc actacgccat
     9421 ttttacaggt cacttttttc acttcatcgc ccggcatcca cacgccaccc tggcgcacaa
     9481 actggcgctg taactggttt tgcagacgaa tgcccagcac ggaaggcggc agcgttggca
     9541 aaagcatcag tgaacaaagt agtttttcat tcaaccaacg ccacagtttg tcatcggcca
     9601 ggccgaagca ggcaggcatc aggatcattt cgcaggtatt ggcgacagga ataagcgcat
     9661 caagtaacag cggccagttt tcttcattat caaggaaacg ggcgatattc acagcgcgaa
     9721 attcggtggc gttattgcgc aggacatcca gttccggcag ctctatttct gcggtttcaa
     9781 ccgaaagatc cagttcgtgc aacgaagccg ccgcaagatg cgcctgaaaa tccatcaggc
     9841 cgcttatccc gaccacacat attttcttcg cgggcaacgg ccagacgggg acttctggcg
     9901 aacttagcca ggtagagcgc agagtgccga gcggcgtaat ccgctggtga gccagttcta
     9961 cgctgccctg caattgcgca ccgctttcag cgattaatgc ctgcgcctgg caagcgagat
    10021 cgagcacgcg ttgtggcccg agaagagagt aaggatgtgc aggtgcctgc tgacgcaaag
    10081 attccagtcc actgtgaatg tctgccacgg gttgaccatc tggcagatgg ctcagcaaat
    10141 ccagcgaacc ggatgagaaa tgcaacgcgc tttgaccacg cgtgacaatg gcacagcgca
    10201 ggccgtgttt ttgcagttgc aggccacaga gtaatccggc gaggccgccg cccataatga
    10261 cagtatcaaa gcgcatcttt ctgctccttc tccagaccac acagtccctg ataaacccag
    10321 cgggtaaatt cgctttcgcg cagtgcgtct ccccaggcga tgggttgcac gcctttccag
    10381 cgttcgttaa ggaaggtgga aagttgctcg atagattgcg cggacgtcgt gacgttaaac
    10441 cgttgcagca gtccggcagc gcggcaggcg cagagttcgc cctggcaggt gcccatcccc
    10501 acacgggtac gacggcgtaa atccagcagg ctattaacgt ttaaattttc taccgcgtac
    10561 tgcacttcac ccgcagttac cgcttcgcac tcacatacca ggctacggtg cagacggcct
    10621 tcgctcagcc aggcaggcgt gcgatcgcca tgacgataaa ccgcagaacc gcgcagcggg
    10681 gcaggcaggg agatgacttt acgcaaggta acttcagcgg gttcttgtga accaggcagt
    10741 gccagatcgg cagtcgtaca ggggcgcgtg ttgcccagtt tgcggcatac cgcgtcggta
    10801 gcccattcag ccatcagccg ataggtcatc agtttgccac cggtgatggt gataaatccg
    10861 tccagaccat cgcgttcagc atggtcgagc agcacgatgc cacggctgac gttacgtccg
    10921 ctcggatcgt catcgctggc aaccagcggg cgcacgccag aataggcccg caaaatgcgc
    10981 gttttcgcca tcacgggggc cagtttttcc ccttcacgca gcagaatatc aacctcttct
    11041 gccgtcactc gattatcgtc aatctcgttg taatcaatac gtaaagaggt ggtaccaatc
    11101 agcgaaatgg tatcgccagg caccagaata tcggcgtcgg aaggtttacg gcagcggttg
    11161 atcacatgct ggttaatgcg gtgatccatg atcagcagcg atcctttcgc cgggaacatg
    11221 cgaatgcgca gatcggcata ttcggcaatg tgttgccccc agatcccagc ggcattaacc
    11281 acgacaggtg catgaagggc ctgagtttcg ccggtgagat ggttacgtac acgaacaccg
    11341 cacaccgtcg cgccttcacg aatcagcccc gtgacttcat gagcggtaag gataacggca
    11401 ccgtgttctt tggcatccag catgtttgct gcggtcagac gaaatggatc aacggtgcca
    11461 tccggaactt tcaccgcgcc aatcagtgcc gggttaacgg caggttcgat aatgcgcgct
    11521 tgctgcgggt ctatagcttc tgcgctgatc cctgcttctt cgcaggcgcg aataaaagtg
    11581 gcctggaatg agaggtcatc ttccggcagg gtgataaaca ggccgttggt tggttcaacg
    11641 cagtgacgtg caatgcgttt caggatctgg ttttcactaa tgcattcgcg ggccgattcc
    11701 gcatcggtta ccgcatagcg cgcaccgctg tgcagcaggc cgtggttacg cccggtggca
    11761 ccggttgcga tgtcgtggcg ctcaaccaaa atcacgcgca gcccgcgcag ggcacagtcg
    11821 cgggcaatcc cggctcccgt tgcgccgccg ccaatgataa tcacgtcact tgattgcgag
    11881 tcgcgagttt tcattgttat ccctctgaag ttcgtttttt accatttagc catagtaaaa
    11941 acatgaattg tttgatttcg cgcatgttcg ctcataattc gaaagtgaaa cgtgatttca
    12001 tgcgtcattt tgaacatttt gtaaatctta tttaataatg tgtgcggcaa ttcacattta
    12061 atttatgaat gttttcttaa catcgcggca actcaagaaa cggcaggttc tctcactgaa
    12121 tcaggctgtt aatcataaat aagaccacgg gccacggagg ctatcaatgt tgagtatttt
    12181 taaaccagcg ccacacaaag cgcgcttacc tgctgcggag atcgatccga cttatcgtcg
    12241 attgcgctgg caaattttct tggggatatt ctttggctat gcggcttact atttggttcg
    12301 taagaacttt gcgcttgcta tgccttatct ggtcgagcag ggattctctc gcggtgattt
    12361 aggtttcgcg ctttcgggga tctcgatcgc ttatggattt tcgaaattca taatgggttc
    12421 ggtatcagat cgctcgaatc cgcgcgtttt cctccccgca ggtttgattc tggcggcggc
    12481 agtgatgttg tttatgggct ttgtgccatg ggcgacgtcg agcattgcgg tgatgtttgt
    12541 acttttgttc ctctgcggtt ggttccaggg gatggggtgg ccgccgtgtg gtcgtactat
    12601 ggtgcactgg tggtcgcaga aagaacgtgg cggcattgtg tcagtgtgga actgtgcgca
    12661 caacgttggt ggtggtattc cgccgctgct gttcctgctg gggatggcct ggttcaatga
    12721 ctggcatgcg gcgctctata tgcctgcttt ctgcgccatt ctggtggcat tattcgcctt
    12781 tgcgatgatg cgcgataccc cgcaatcctg tggcttgccg ccgatcgaag agtacaaaaa
    12841 tgattatccg gacgactata acgaaaaagc ggaacaggag ctgacggcga agcaaatctt
    12901 catgcagtac gtactgccga acaaactgct gtggtatatc gccatcgcca acgtgttcgt
    12961 ttatctgctg cgttacggca tcctcgactg gtcaccgact tatctgaaag aggttaagca
    13021 tttcgcgctg gataaatcct cctgggccta cttcctttat gaatatgcag gtattccggg
    13081 cactctgctg tgcggctgga tgtcggataa agtcttccgt ggcaaccgtg gggcaaccgg
    13141 cgttttcttt atgacactgg tgaccatcgc gactatcgtt tactggatga acccggcagg
    13201 taacccaacc gtcgatatga tttgtatgat tgttatcggc ttcctgatct acggtcctgt
    13261 gatgctgatc ggtctgcatg cgctggaact ggcaccgaaa aaagcggcag gtacggcagc
    13321 gggctttacc gggctgtttg gttacctggg cggttcggtg gcggcgagcg cgattgttgg
    13381 ctacaccgtg gacttcttcg gctgggatgg cggctttatg gtaatgattg gcggcagcat
    13441 tctggcggtt atcttgttga ttgttgtgat gattggcgaa aaacgtcgcc atgaacaatt
    13501 actacaaaaa cgcaacggag gctaatggca tgaaattgac gctgaaaaac cttagcatgg
    13561 cgatcatgat gagcactata gtcatgggaa gcagtgcaat ggcggcggac agcaacgaaa
    13621 aaatagtcat cgcccatcgc ggtgccagtg gatatttgcc ggagcatacg ctgccagcaa
    13681 aagcgatggc gtatgcgcag ggagcggatt atctggaaca ggatttggtg atgaccaaag
    13741 acgaccatct ggttgttctg catgaccatt atctcgatcg tgttactgat gttgccgatc
    13801 gtttcccgga tcgggcgcgc aaagacggtc gttactacgc gatagatttc acgctggatg
    13861 aaattaagtc gttgaaattt accgaaggtt tcgatattga aaacggtaaa aaagtgcaga
    13921 cttatccggg gcgtttccca atgggtaagt ccgacttccg ggtgcacacc tttgaagaag
    13981 agattgaatt tgttcagggg ttaaatcact ctaccgggaa aaatatcggt atttatccag
    14041 aaatcaaagc gccgtggttc catcatcagg aagggaagga tattgcggca aaaacgctgg
    14101 aagtgctgaa gaaatatggt tacaccggta aagacgataa agtttatttg caatgttttg
    14161 atgctgatga gctgaagcgt attaagaatg agctggaacc caaaatgggc atggagctca
    14221 atctggtaca gctgattgcc tataccgact ggaatgaaac gcagcagaaa cagccggatg
    14281 gaagctgggt taattacaac tacgactgga tgtttaagcc gggtgccatg aaacaggtgg
    14341 cggaatatgc agatggtatt ggtccggatt accatatgtt gattgaggag acatcgcagc
    14401 cgggtaatat caaactcact ggcatggtgc aagatgctca gcagaataaa ctggtagtgc
    14461 atccttatac cgtgcggtca gataaactgc ctgaatacac tcctgatgtg aatcagttat
    14521 atgatgctct gtataacaaa gcgggtgtaa atgggctgtt tactgatttc cctgataagg
    14581 cagtaaaatt tcttaataaa gagtaatgtt actgcaggat ggcgttcatg cgccatcctg
    14641 tctcatatca aatttttcca atcgccacta attgttccat gcacatttgt agtaaaaaca
    14701 gtggactgcc ttctaccggc ttttctttag ccagcaacga aatctgtcga tagaaagttg
    14761 gttcgagagg aacggaacac aattcacctg caatgctttt cagcgttaac tctggttgta
    14821 aggcgatgcc taatccctgt cgaataaaat tcatcgctgt atccgcctga ttaaattcac
    14881 attttatctg ctgggcgcac tattagtggt agtggctggt ggaatctatg cgctgcaaat
    14941 gaagggtaaa agcggtgtca agctagtagt ggcaaaagaa attcattaat acgacacgtt
    15001 tcattaagat tttcctcagg taaccaccta tagtcattgt cgttaataaa aacgacaggc
    15061 gatagggtat ggcagtttca gcaaagtacg acgaatttaa tcactggtgg gcaacagagg
    15121 gcgactgggt tgaagagcct aactatcgcc gtaatggtat gagcggggtg caatgcgtcg
    15181 agcgcaacgg caaaaagctg tatgtaaagc gcatgacgca tcatctgttt cattctgtac
    15241 gttatccgtt cggccgacca acgattgtcc gtgaggttgc ggtaattaaa taactggaac
    15301 gggccggtgt catcgtgccg aagatcgttt ttggtgaagc ggtgaaaatt gagggtgaat
    15361 ggcgagcgtt gctggtgact gaagatatgg cggggttcat cagcattgct gactggtatg
    15421 cccagcatgc agtatcgcct tattctgacg aagtacggca agccatgttg aaagcagtgg
    15481 cgctggcgtt taagaaaatg catagcatta atcgtcagca tggctgttgt tatgttcgcc
    15541 atatctatgt gaaaacagaa ggtaaggcag aagctggttt tcttgacctg gagaaaagtc
    15601 gtcgccgctt gcgtcgcgat aaagcgatta atcatgactt ccgccagctg gaaaaatatc
    15661 tggagccaat ccccaaagcg gactgggagc aggtgaaagc gtattactac gccatgtaat
    15721 cgttgtaggc cagataagac acataagcgt tgtatctggc actacaacca attcacatct
    15781 cgatttcaat atcgcctttt gcccggcaac aacagggcaa aatttccccc ggctgaataa
    15841 aggctaacgg ttcggcaatc cagtcaactt gacctgcaac caggcgtgtg cgacaggagc
    15901 cgcagtaacc ttcgcgacac tggtactcaa ccgccacatt gtgggattcc agcgctgcca
    15961 gaagggaagg gtgttcatcc tggcacagca gttgtgtgcc agtgatgcgc agggtaacgc
    16021 gggccatcag agctggaagt tactcaaatc gtcggtgtcc acttccgagt caatctgccc
    16081 gaccagataa gaactgactt ccacttcctg cggagcaacc tgcacgttat cagacaccag
    16141 ccaagtgttg atccacggga tcgggttgga gcgcgtctgg aacggcagat ccaaaccgac
    16201 tgcctgcata cggatattgg tgatgtattc aacgtactgg cagagaatgt ctttattcag
    16261 accaatcatc gaaccgtcgc ggaacagata atccgcccag tctttctcct gttgagctgc
    16321 ctgaacaaac aggtcatagc actcctgctt acactcttcg gcaatttccg ccatctcagg
    16381 atcgtccgcg ccgctgcgca gcagattcag catatgctgg gtgccggtca ggtgcagggc
    16441 ttcgtcgcgg gcaatcaggc gaataatttt ggcgttgcct tccatcaatt cgcgttctgc
    16501 aaaggcaaag gaacaagcaa agctgacgta gaagcggatc gcttccagcg cgttaacgct
    16561 catcaggcaa agatacagtt ttttcttcag ctcgcgcagg ctaacggtta cggttttacc
    16621 gttaacggtg tgggtgcctt cgcccagaag gtgccagtag ctggtcattt cgatcagttc
    16681 atcgtaatag ctggagatcc cttccgcacg tttctggatc tgctcgttgg tgacgatatc
    16741 gtcaaacaca acagacggat cgttaacgat attacgaatg atatgagtat aggaacggga
    16801 atgaatcgtt tctgagaacg cccaggtttc gacccaggtt tccagttccg gaatagaaat
    16861 aagcggcaat agcgccacgt tcggactacg accctgaatg gaatccagca gcgtctgata
    16921 tttcaggttg ctgataaaaa tgtgtttttc gtgctccggc agcgcctggt aatcgatacg
    16981 gtcgcgggag acgtcaactt cttccggacg ccagaagaaa gagagctgct tttcgatcag
    17041 cttttcgaag atgtcatatt tttgctgatc gtagcgggcc acgttgaccg gctgaccaaa
    17101 gaacatcggt tctttgagct gatcattttt cgtctgtgaa aaggtggtat atgccatgag
    17161 tgtgtcctgt tgggagttta atgccggata aggcatttta cgccgcatcc tgtgcctgat
    17221 gcgacgctgg cgcgtcttat caggcctaca aatccgagcc gtaggccgga taaggcgttc
    17281 acgctgcatc cggcatctca atatcagatc ttacatgcgc cgctttcgca gccatcgtcc
    17341 tggattgacg gtaccagatc gtcctgcgcg tcttcagcgc cgtcacgggt gttctgataa
    17401 tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt ctttcagcaa ctgctgcatc
    17461 ggcacttttc ctgacgggaa acgtgacgga tcgtagttgg tgttagcaga gatcgactga
    17521 tcgataaatt tctgcatgat acctaccagt tgcaggtagc catcgttacc cggcatttcc
    17581 cacagcagct cataggcgtc gtgcaggtgc tcgtagtccg gcaccacctg gcgcaaaata
    17641 ccgtctttcg acgctttgat gctgacgtaa ccgcgcggcg gttcaatacc gttagtggcg
    17701 ttagagatct gcgaagaggt ctcggacggc atcagggcag aaagcgtgga gttacgcagg
    17761 ccgtgcgttt tgattgattc acgcaatgct tcccagtcgt aatgcagcgg ctcattagcg
    17821 atggcatcca gatctttctt ataggtatcg atcggcagga tccctttcgc gtaagtggtt
    17881 tcgttaaacc acgggcactc gccttgctct ttagccagct cattagacgc tttcagcagg
    17941 taatactgaa tcgcttcgaa ggttttatgc gtcaggttgt tggcgctgcc gtcggagtag
    18001 cgtttaccgt gcttcgccag gtagtaagcg aagttgatca caccaatacc cagcgtacga
    18061 cgacccatcg ctccacgttt ggcggccggg atcgggtaat cctgataatc cagcagcgcg
    18121 tcaagtgcac gaaccgccag aattgccagc tcttccagtt catccaggtt attaattgcg
    18181 cccaggttga aagcagacag cgtacacagc gcgatttcac cgttctcgtc gttgacgtcg
    18241 ttcagcggtt tggtcggcag ggctatctcc aggcacaggt tagactgacg cactggcgcg
    18301 atggccggat caaacgggct atgggtatta cagtggtcaa cgttctgaat atagatacga
    18361 ccggtagacg cacgttcctg catcatcagc gagaacagct caacggcttt cacacgctgc
    18421 ttgcggatgc tgtcgtcttt ctcatatttg gtatacagac gttcaaactc ttcctgatcg
    18481 gcgaagaacg cgtcgtacag ccccggtacg tcggacgggc tgaacagggt gatatcttca
    18541 cctttcagca gacgggtata catcagtttg ttgatttgta ccccgtagtc catatgacgc
    18601 acgcggttgc cttccacacc acggttgttt ttcaacacca gcaggctttc cacttccaga
    18661 tgccacatcg ggtagaacag cgttgccgca ccgccgcgca caccgccctg agagcaggat
    18721 ttcaccgctg tctggaaatg tttgtagaac ggaatgcagc cggtatggaa cgcttcacca
    18781 ccgcgaatcg ggctacccag cgcacgaata cgcccggcgt tgatgccgat cccggcacgc
    18841 tgggaaacgt atttaacaat cgcgctggag gtggcgttga tggaatccag gctgtcaccg
    18901 cactcgatca gtacgcagga gctgaactga cgagtcgggg tacgcacgcc ggacatgatt
    18961 ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt aaaaacgctt cacatattgc
    19021 aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg caaccagaat ataaaggaac
    19081 tgggcgctct catagatttc gccggtcacg cggttctgta ccagatattt gccttccagc
    19141 tgcttaacgg cagcataaga gaaggtcata tcacggtcgt gatcgataaa cgtgtccatc
    19201 tgcttgaact cttcttccgt gtagtcttcc agcagatgat tatcgtattt gcccatctcg
    19261 accattttca ccacgtggtc gtacagcgca ggcggctcaa actggccgta ggctttttta
    19321 cgcaggtgga agatcgccag gcgcgcggcg agatactgat aatccggcgc atcacgggag
    19381 atcaggtctg cggcagcctt gataatggtt tcgtggatgt cagaggtctt gataccgtca
    19441 taaaactgaa tgtgggagcg cagctcgacc tgggaaatcg aaacgttatg cagtccttct
    19501 gccgcccaat ccagaacgcg atggattttg tcgagattga tgcgctctgt gctaccgtcg
    19561 cgctttgtca ccagcagatt ctgattcatg tatgtcgtac ctgtttttgg aattctttcc
    19621 gcaatagttt catgcagact acctgtagta cgggaagctg cttagaacac tatatatagg
    19681 gggtatgttt gggattcact gcaagatagt gtgaaaatgg ccctcttgca agtgcataac
    19741 tttgtggata actcaggaag gaaaaagtgg ctttcgcgca ccttaggtca gacaaggtgt
    19801 ccgggaaagt caatgggaag agaaaaattt gttaaaaata actgttcggc gatttcttga
    19861 acggtagaat aggttggata aattgatata gatggtttat taat
//
LOCUS       DAJTYD010000061        19869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTYD010000061 DAJTYD010000000
VERSION     DAJTYD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.061"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(52..1079)
                     /locus_tag="OO585_001973"
                     /pseudo
     CDS             complement(52..1079)
                     /locus_tag="OO585_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(1089..1901)
                     /gene="hcaB"
                     /locus_tag="OO585_001974"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(1089..1901)
                     /gene="hcaB"
                     /locus_tag="OO585_001974"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6629572.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(1898..2218)
                     /locus_tag="OO585_001975"
     CDS             complement(1898..2218)
                     /locus_tag="OO585_001975"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6629573.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            complement(2218..2736)
                     /locus_tag="OO585_001976"
     CDS             complement(2218..2736)
                     /locus_tag="OO585_001976"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6629574.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRPRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2733..4094)
                     /locus_tag="OO585_001977"
     CDS             complement(2733..4094)
                     /locus_tag="OO585_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6629575.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            4230..5120
                     /gene="hcaR"
                     /locus_tag="OO585_001978"
     CDS             4230..5120
                     /gene="hcaR"
                     /locus_tag="OO585_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6629576.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            5280..6419
                     /locus_tag="OO585_001979"
     CDS             5280..6419
                     /locus_tag="OO585_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6629577.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(6411..7691)
                     /gene="csiE"
                     /locus_tag="OO585_001980"
     CDS             complement(6411..7691)
                     /gene="csiE"
                     /locus_tag="OO585_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6629578.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(7882..8736)
                     /locus_tag="OO585_001981"
     CDS             complement(7882..8736)
                     /locus_tag="OO585_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6629579.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(8881..9684)
                     /gene="suhB"
                     /locus_tag="OO585_001982"
     CDS             complement(8881..9684)
                     /gene="suhB"
                     /locus_tag="OO585_001982"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6629580.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAVEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9803..10543
                     /gene="trmJ"
                     /locus_tag="OO585_001983"
     CDS             9803..10543
                     /gene="trmJ"
                     /locus_tag="OO585_001983"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6629581.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10813..11301
                     /gene="iscR"
                     /locus_tag="OO585_001984"
     CDS             10813..11301
                     /gene="iscR"
                     /locus_tag="OO585_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6629582.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            11413..12627
                     /gene="iscS"
                     /locus_tag="OO585_001985"
     CDS             11413..12627
                     /gene="iscS"
                     /locus_tag="OO585_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6629583.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            12655..13041
                     /gene="iscU"
                     /locus_tag="OO585_001986"
     CDS             12655..13041
                     /gene="iscU"
                     /locus_tag="OO585_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6629584.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            13058..13381
                     /gene="iscA"
                     /locus_tag="OO585_001987"
     CDS             13058..13381
                     /gene="iscA"
                     /locus_tag="OO585_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6629585.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            13477..13992
                     /gene="hscB"
                     /locus_tag="OO585_001988"
     CDS             13477..13992
                     /gene="hscB"
                     /locus_tag="OO585_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6629586.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            14009..15859
                     /gene="hscA"
                     /locus_tag="OO585_001989"
     CDS             14009..15859
                     /gene="hscA"
                     /locus_tag="OO585_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6629587.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            15861..16196
                     /gene="fdx"
                     /locus_tag="OO585_001990"
     CDS             15861..16196
                     /gene="fdx"
                     /locus_tag="OO585_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6629588.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            16208..16408
                     /gene="iscX"
                     /locus_tag="OO585_001991"
     CDS             16208..16408
                     /gene="iscX"
                     /locus_tag="OO585_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6629589.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            16586..17869
                     /gene="pepB"
                     /locus_tag="OO585_001992"
     CDS             16586..17869
                     /gene="pepB"
                     /locus_tag="OO585_001992"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6629590.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            18011..18787
                     /gene="sseB"
                     /locus_tag="OO585_001993"
     CDS             18011..18787
                     /gene="sseB"
                     /locus_tag="OO585_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6629591.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(19280..>19869)
                     /gene="sseA"
                     /locus_tag="OO585_001994"
     CDS             complement(19280..>19869)
                     /gene="sseA"
                     /locus_tag="OO585_001994"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6629592.1"
                     /translation="DKHLIVYDEGNLFSAPRAWWMLRTFGVEKVSILGGGLAGWQRDD
                     LLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHENTAQIIDARPAARFNAEVDEPR
                     PGLRRGHIPGALNVPWTELVREGELKTTDELDAIFFGRGVSYDKPIIVSCGSGVTAAV
                     VLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         4729 a         5067 c         5409 g         4664 t
ORIGIN      
        1 tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc agtcatcgcc
       61 acgcatatcg ccaataaact gtaagttatc actgtactga tcgctccaga accacggcag
      121 tagcggtagc ggtagcccta acattgcggc agcggcaatt tgcgcctggt tattggcgtt
      181 ttcccagctt tcacagcggt gtagtgcacc attatcaaga cgagtgattg ccacatcgcc
      241 accggcaaag atcgcgggat cgcaggtgcg gcaagcctca tcaatgacaa tgccattggc
      301 agtatcaagg ttggcctcgc gagccagttg ctcgttggcg ctgataccaa taccgtaaat
      361 caccacatca gcctgaagcg tttccccact ttgcagcgtc agttctactt tttcaccatc
      421 gaccacatgt tcaatggcat tattgagcag aatgcgcaca ccagcctgct ggtggcgctg
      481 taaaagatag cgttgcacgg gcggtggtgc attacggccc atgacggttg ccgccagttc
      541 aatcactgtc accttacatc tgcgctgcgc tgcgctgcgc tgcgctgcgt ggcgctggca
      601 gccagttcca gaccaatagt tccggcaccg acaatcacga ctgaccgttc gggctgcaga
      661 acttctcgca gtctggcggc atcaccggca tggcgtaggg taaagcagcg ttctcccagt
      721 gcatcaagca acggcagcgg tcgagctgcc gcgccggttg ctataaaaag ctgatcccag
      781 tgccagcttt cgccgttggt taacactaac tctcgtgtgt cgcgacccag cgttttgatg
      841 gttacaccgg aatgcagatg aacattgttt tcctgccacc agttagcggg taacacctgc
      901 tgtaactgcg gggaatcttc cagcaacatg gatttcgaga gtggaggtcg ttcataagga
      961 agatgtcgct catcggaaaa cagatgcagc tcaccggtga acccttgctg gcgtagcgag
     1021 gccgcagcca ttgccgccgc ttgcacgcca ccgacaataa tgatcgtttt ttctttcatc
     1081 gtgcttcctt aaagatccag cccagccgct acgtggcgaa tgccgcgaat cgctaaaccc
     1141 gcatcagcgt tgatcatcac accgcttaat gcgcgattat tgcgccgcga tgccaacatc
     1201 acatacggcc ccgtaaaatc cgccggttgc gggaaaaatt gcagcggtaa aatggcggca
     1261 attttctccg gcgtcagaga ctgcattatc gaggtttcac tttgcccgag cgcctgtggg
     1321 ccgcgcaggt cgctggccat accacacggg ccgacgccat tcacccgcac tttcggtgcc
     1381 agttcataag ccagttggcg aataagtccg gttgcggcat gtttactggc ggtgtacagc
     1441 gggccaccgc cgccaggata ccaggcggca tttgacagtg tgaaaatcat gctgccttca
     1501 ctggcgatta acgccggagc gcaggctttt gcgcccagca ggtaaccgag tacgttgacg
     1561 ttaaacagct cgtggaagcc ggtttcgagc gtctctgcgg gagtattaac cagtgaggca
     1621 ttgtgatccc agatgcctgc attgccgata aaacaatcca gcttgccgga acgagtcagg
     1681 atctgatcga ccgcgcgttg ataatcggca taacaggtca cgttaccttc caccgccaga
     1741 atatgttcgc caaatcgctg acgcagactg gcgacttttg ccgccgacag ttccagcgtg
     1801 gcaacctgcg cgccttcttc gataaatcgc tcgaccagcg ccagccctaa tcccgatccg
     1861 ccgccggtaa taaaaatgga ctcgttatgc agatcgctca tggctgcgcc tccggtaagt
     1921 cgatgaaaat gtcactacct tcaacgtgta ctggataagt ggtgagcgga tcggtggcgg
     1981 gcaggcataa cgctttcccc gttttcaggc aaaaactggc ggcgtgtagc gggcactcca
     2041 ccgtggcgtc atcttccaga tacccttctg acatcgacgc attaccatgg ctgcaacgat
     2101 cgttaatggc ataaaactcg ccgccaacgt tgaacagggc gatgacgggc gaggtatcga
     2161 tccggagagc ctcaccctcc ggcacatccg caacgggaca cgcataaatt cgattcatca
     2221 gaacagtaca ctcaggttat gggaagtgat taccgcttga tccaggacga tatcccgttc
     2281 cagcaagcgc cagttgtcat cttccagacg gcgaactttg tcgaaacgcg ttccaacata
     2341 gaatgtttca tcgcgctctt tttgtgcctg ataaagcaga taatttaccc gcacagcaaa
     2401 tacgtttggg atgtcggttt cgcttatctg gcagttgctg attaagtgac gggggcgtga
     2461 cggcggctct tctgcccagg ccatgcccgt ttccagacgg gcgattcgcc gctccagctg
     2521 gtctttggtg tcattaaaaa tccaggttgt cggtggctgg acgcctttgc ggcggtcgcg
     2581 tgtttgcgcg ttaactgtgg tgcgcatggt gtaacgaatc tcttcgtcga gctgcgccag
     2641 ccagtcacga aatttccagt cgtccagtaa gctggcttcg tgaaagagaa actggctaat
     2701 gcggagatgt aactctagtg aaacttgcgc actcatttca tcacctcctg ctggtaagcg
     2761 gcggttttat cgagcacttc ctgccagctt tcgctactca gaagatcggc ccagcgttgg
     2821 tacattccac gagcggcagt ttctgaaaag atatagttag taatgccagg aatgccgtcg
     2881 tcgcggcgct tttcctgacc aagccccatt tccagacaca gtttgctgtt gcgggcgcgg
     2941 tggcctttaa gcaatttctg gatttcacac cagttctccg agtcatcctg ctcgagaaaa
     3001 ccagcaggac caaaagcacg agtggcgctg ttttcaaaag cggctttaac ttcatcggag
     3061 gcggctttgt cagtaataca gaacgcccac acttcaactt gatcagggcc gcgcggatgc
     3121 cagacgcgga gtgtggcagt gccgtttagc catgaaagcg tggggaatat attgttatga
     3181 cccgccaggc gcagggcgcg aacttcacct aaacgttgtt ctgcttcggc ataggtttcg
     3241 cggtaatagc tggaaaccgc gccatcgacc cagacattag catccggttt ttcagtaaag
     3301 aagaaaccgc taccgtgacc gtcctgacca aattgcagcg catctttggc ggtttcccac
     3361 accgggcggg cggtttgtcc atcaccgaga cgcttatcgc tgccatcatc tttcgcccct
     3421 aatacctgaa cggcagaagc atggctgaac agagcatgat attggtcact ggcgaactgc
     3481 tctgccgggg atttccagtt acagttgatc acccactttt gtacgccgcc gacaatttcg
     3541 gtgccgcctt cgcgacgatc cagcatgcca tccagatacc aggcaatgtc accaaggtaa
     3601 tcacgcaggc ccggtgcgct ggtatcccag ttgccaaaaa tcagcccttt ataactctcc
     3661 acacaaggaa cttcgtttag cccccagtgg gatttacaca acccttgtgg gtaggcgcga
     3721 ggttccagcg gtacatcgat caactcgccg ttaatgccat aagaccagcc gtgatacggg
     3781 caggtaaagg cgcgagtgtt gccgcaatct gcataactca cacgcatggc ccggtggcgg
     3841 cattggttga gaaacgcctt gatgctgccg tctttctgac gcactacgac aaccgcatct
     3901 tctcccatgt aggtgttaaa gaaatcaccg ggttttggga tctggctttc gtgggcgaga
     3961 aatagccagc aacgaccgaa aatacgctca agctccagtt ggtaaatatc cgggtcggta
     4021 taaatacgcg gagtgacccg accattttgg gtgtcaatca gttggtaaat gttcaaatct
     4081 gagggtgtgg tcatattttt taatcctgaa agataagatg tattgacctt ctatttttgt
     4141 gccataagga tgttgctggt tggttgctaa tatgtgaaat agttaatttc taccagctga
     4201 gacgtttttt cgataacaag gggaaggtga tggaactacg gcatttacgc tatttcgtcg
     4261 cagtggcgca ggcactgaac tttacccgtg cggcggaaaa actgcatacc tcacagcctt
     4321 cgttaagcag ccagatccgc gatcttgaaa actgtgtcgg tgttccgtta ctggtgaggg
     4381 ataagcgcaa agtcgcgttg acggcggcgg gagaatgttt tctccaggac gcgctggcaa
     4441 tcctcgaaca agcggaaaat gccaaattac gggcgcggaa aattgttcag gaagacagac
     4501 aattaaccat tggctttgtg ccatcggcgg aagtgaattt actgccaaaa gtattaccga
     4561 tgtttcgtct cagacagcca gacaccttga ttgagctggt gagtttaatc accacgcaac
     4621 aggaggaaaa aattcgccgt ggtgaactcg acgtcggctt aatgcgccat cctgtttata
     4681 gcccggagat tgattatctg gagctttttg acgaaccatt agtggttgtg ttaccggttg
     4741 atcacccttt agcacatgaa aaagagatca ccgccgcgca actggatggc gtgaatttcg
     4801 tcagtaccga tccggcgtat tccggttcgc ttgcgccgat cgttaaagcg tggtttgcgc
     4861 aagaaaacag ccagccaaat atcgtccagg tggcaacgaa tattctggtg accatgaatc
     4921 tggtgggcat ggggctgggc gtcactttga tacccggtta tatgaataat tttaataccg
     4981 gacaggttgt ttttcgtccc attgccggaa atgtgccttc cattgcttta ctgatggcgt
     5041 ggaagaaagg ggagatgaag ccggcattac gcgatttcat cgccattgtg caggaacgtt
     5101 tggcaagcgt aacggcataa ttacgcatta tttttgcaac atgcgtcgcg gatcgcagtt
     5161 ttttagtgct tgtgaactgg cgttacagac atcgggaagc aggctcgaat attcttattt
     5221 ttcaacttgc accaacattc gattaacccc agactaacgc ctcctgacgg gagggactca
     5281 tggttttgca atccacgcgc tggttggcgc tcggctattt cacatacttt tttagttacg
     5341 gcatttttct acctttctgg agcgtctggc ttaaagggat tggtttaacg ccagaaacca
     5401 tcggcctgtt attgggggca ggtctggttg cccgttttct cgggagtttg ctcatcgcgc
     5461 cccgcgtcag cgatccttct cgcctgattt ccgccttgcg cgtgctggca atgatgacac
     5521 ttctctttgc tgtcgccttc tgggcggggg cgcacgtagc gtggctgatg ctggtgatga
     5581 ttggctttaa cctctttttc tcaccgctgg taccgttgac cgatgcactg gcgaatacgt
     5641 ggcaaaagca gttcccgctt gattacggca aagtgcgact gtggggctcg gtggcgtttg
     5701 tcattggctc ggcgctgacg ggcaaactgg tcactatgtt tgattatcag gtgatcctcg
     5761 cgctgttaac attgggcgtg gcatccatgc tgctcggctt tctcatccgt ccgacgattc
     5821 agccacaagg ggcaagccgc cagcaggaga gcaccggttg gtcagcgtgg ttggcgctgg
     5881 ttcttcagaa ctggcgcttt ctggcctgcg tttgtttatt gcagggggca catgcggcct
     5941 attacggttt tagcgccatt tactggcagg cagctggcta ctcggcctcg gcggtggggt
     6001 atttgtggtc gctgggcgtg gtggcggaag tcattatctt tgcgctgagt aataaacttt
     6061 tccgccgttg tagtgcacgc gatatgctgt tgatctcggc gatttgcggc gtagtgcgct
     6121 ggggcattat gggagcaact acggcgttgc cgtggttgat agtggtgcaa attctgcatt
     6181 gcggcacctt cacggtctgc cacctggccg ccatgcgcta tattgccgct cgccagggta
     6241 gcgaagtcat ccgtttacag gcggtttact ctgccgtcgc gatgggcggc agtatagcta
     6301 tcatgaccgt tttcgccggt ttcctgtatc aatatctggg ccacggcgtg ttctgggtaa
     6361 tggcgctggt ggcgcttccg gcaatgtttt tgcgcccgaa agttgttccc tcatgctgat
     6421 tcgagcattt tgcggatctg ctcctgttga tgtgttgtca gtgtcaggtc cgtatagatc
     6481 agcggtggtg aaaagagcgg taacggcatg gtataaggcg caataatcag tgccgcgccg
     6541 cgcggagcgc ctgtctgcaa aaatgccttt accgacatat gtttaatgtt gagcggcagt
     6601 aacgttagtt cgcgtaactg ctgctcaatc tgcgcttctc gctcgctatc attcccggta
     6661 agaagaataa tctgtttttc atgcagatcg ttttcctgca ttagccaggc accgaaaatc
     6721 accgcgacca gaccactttc ctcatcagaa agatggacgc cgtattcact ttcaaatccg
     6781 gccagcgccg cgcgggtggt gcgcaccagg cgtgggtaca gtctggcgaa ctcttctggc
     6841 agggtattat caataccgat ggcaaacaaa ctgcgattta acgcctgggc gaggtgggta
     6901 taaagctgat cgcataaccc ctgttcatca taaaaacgaa catttcccag ctcacgaaaa
     6961 tggtttacca gacgtttgat agactgacgc aattgtctgt ctcgctgatg cgcatcacgc
     7021 aatggatcgg gaacccgcaa catggaaaaa agtaatgcca taaacagtgg ctcatcaggc
     7081 ggtacaggct ggagcgcccg acgctgccag tggcgtccaa tttcctgcgc tacctgaaat
     7141 tcaaggcagg attccgccca gcgacgttgg agcggattaa actgaggcgt aattccagcg
     7201 tggtgttgca gcaaacaata ttgcagatac agacacagga aatgaatatc gcgcgtttca
     7261 aagcgttttt gcagccgtcg ggagcagaga ttcactaacg cttgtagatt ggtatcgtcg
     7321 taaaagttac gcgcaattcc gcgccgcttc agttcactct tcagggcggg ggtgaattgg
     7381 ctggtaataa atgacggaca cagacggaaa ccacgtcgta gccagtgaaa taaacacaaa
     7441 cgttggttaa gcactgtacc ttcaacccgg tagctaccgt cataaccagt cgtcagtgtg
     7501 agttgatgat agcgcaggat ctcccgtcct gtttcgctga tatcaagact ggcaatatca
     7561 tcatccacac cattaagctc gctgaaggtt gccatggtgg cggtcaaccc cggctgaaag
     7621 agcgtcagca agatctggca gcggcgctgg ggagccgaaa ggacagatgg tggagcaagc
     7681 gtaggcatca tctgtagatc tcccataagc ataattgata agaatagcta aagtttgctc
     7741 aggtaaatgg cgaagggaat gctaatcatc agaaatgttg gcaagcgtca cagaattttt
     7801 ctcatcttaa actgttctga ccagtggatt agcatgtctt aaggtggttt tgtgaatgat
     7861 tatcgtgcta gcgatcatgg gttagttctg cggattcaac gcgctaagga taaagtccac
     7921 catctgttcg cgataaacat cgccgtgacg atcggaaaaa gcatcaatgt gttcgccatc
     7981 cgggattagg atcaaccgtt ttgtctcttt tgccaggcta tacaactttt cgctgtgctg
     8041 ccatgggata acgtgatcag ctttaccgtg aatgagtaaa agcgggatcg ggctgacgct
     8101 ggcgatataa ttttcgccgc tgtaactctc atcaagtaag tagccactgc cggggatcat
     8161 ctgattggcg atggttgcat aagaggcaaa tgtggagtcg aggatcaccg cacgtatgcc
     8221 ttcacgatca tcctgaccaa taacagcaag aatattcgcc ccgccaatac tctgcccgaa
     8281 cagcaccagc cgttgtgggt ttacatcact gcgatggcgc accacattga tggcactttg
     8341 cgtatcgtcc agcaatccgg cctgggacgg cgtgcctttt gatttaccaa acccgcgata
     8401 atcaaacata aaaacgttga aattacgctc gggtaaccaa ctgaccagcg gccagtgggc
     8461 ggacatattt ccggcattgc cgtgagcatg aatgatggtt gcgatggcat tgtcagcagg
     8521 gcccgtcgaa gaaggaataa accagccttg cagacgagta ccgtccttag ccgtaaattc
     8581 gacggattcc gccgaccagg ggtcgggacc gtaaatttta tcgtcaggat aatagaagaa
     8641 gttgatggca acgcgcggca ctaaataaac gcagaaggca acaacaaaga gaataaacag
     8701 aattttgaga acgcgtttgt tcactggcag cgccataacc tttccttgtg acttctttat
     8761 catccattaa tcggggaaag atggtgggag agtgacgaaa gaaagtaaag cggtgaggaa
     8821 gtatttcgga atagcgcctg aaaggcgagg gcgggtgagt gacatcaccc gcctgagtca
     8881 ttaacgcttc agagcgtcgc ttaactcatc acgcatgttc gccagcatgg ctttcacaac
     8941 acgcgggtta ccagcaacga tgttaccggt cagcatatag ttatgaccac cggtgaagtc
     9001 gctgacgatg ccgcccgctt cacgaaccag cagctcgcct gccgcgaaat cccacggacg
     9061 cagaccgatt tcaaagaaac cgtcaacacg acccgcagcg acgtaagcca gatccagcgc
     9121 cgcagaaccg gtacgacgga agtctgcaca ttcgttgaac agtttgccga cgatgttgat
     9181 gtaggtagtg gcgtactgtt ttgctttgaa cgggaagccg gtcgccagaa tggtaccgtc
     9241 gagatcgcga gcggtgctac cgcgcagacg gtagccgttc agctgtgcgc cctgaccgcg
     9301 agtggcggtg aacagttcgt tacgcatagg atcgtatacc acagcaactt cggtgcggcc
     9361 tttgatacga acggcgatag ataccgcgaa gtgcggcaga cgtttgataa agttggtagt
     9421 accatccagt ggatcgataa cccattgaac atcctgatca gtaccttcaa gttcaccgct
     9481 ttcttcggtg atgatggtgt gctgtgggta agatttacga atcgtgtcga taatcaccgc
     9541 ttcgacagct ttatctacgt tggtcacgaa atcgttactg cctttctggc tcgcttctac
     9601 agcgtccggg gtttcatagt ttttggcaat taaattaccc gccttgcgcg ctgcgcgcac
     9661 ggcgatgttc agcatcggat gcatcggtct ctctcactgg atgttaaaga acgggaaaac
     9721 ggcgcagagt atagcagcga gatcggcatc tgtcttcgtt ttatgataag atgcgtgaat
     9781 aatctttaga tgaagaaaga caatgctgca aaatattcga attgtgctgg tggagacgtc
     9841 acacaccggc aatatgggtt ctgttgcccg tgccatgaaa acaatgggat taaccaatct
     9901 gtggctggtt aatccactgg tgaaacccga ctcccaggcg attgccctgg cagcaggggc
     9961 cagcgatgtg attggtaatg ctcacatcgt cgatacgctc gacgaagcgt tagccggttg
    10021 tagcttggtg gtgggcacca gcgcacgttc ccgcacgctg ccatggccga tgctcgaccc
    10081 gcgcgaatgc ggcctgaaaa gcgtcgctga agcggcaaat accccggtgg cgctggtgtt
    10141 tggtcgcgag cgcgtcggct tgaccaatga agagttgcag aaatgccatt atcatgtcgc
    10201 gattgcggct aacccggaat acagctcgct gaacctggcg atggcggttc aggttattgc
    10261 ctatgaagtg cgcatggcct ggctggcaac tcaggaaaac ggcgagcagg tcgagcatga
    10321 agagacgccg tatccgctgg tcgatgatct ggagcgtttt tatggtcatc tggaacaaac
    10381 gctgctggca accggtttta tccgtgaaaa ccatccgggg caggtgatga ataaattgcg
    10441 ccgtctgttt acccgtgcgc gcccggaaag ccaggaattg aatatcctgc gcgggattct
    10501 ggcttctatt gagcagcaga ataaaggtaa caaggccgaa taacagtagt tgcctgatgc
    10561 gacgcttaac gcgtcttatc aggcctacaa tgtacagaac cgtaggtcgg ataaggtgtt
    10621 cacaccgcat ccgacaacag gtacaaacgc cacgataaaa aaatggcact gaaggttaaa
    10681 tacccgacta aatcagtcaa gtaaatagtt gaccaattta ctcgggaatg tcagacttga
    10741 ccctgctatt caataccccc acttttacaa taaaaaaccc cgggcagggg cgagtttgag
    10801 gtgaagtaag acatgagact gacatctaaa gggcgctatg ccgtgaccgc aatgcttgac
    10861 gttgcgctca actctgaagc gggcccggta ccgttggctg atatttccga acgtcagggc
    10921 atttcccttt cttatctgga acaactgttt tcccgtctgc gtaaaaatgg tctggtttcc
    10981 agcgtacgtg gaccaggcgg tggttatctg ttaggcaaag atgccagcag catcgccgtt
    11041 ggcgaagtga ttagcgccgt tgacgaatct gtagatgcca cccgttgtca gggtaaaggc
    11101 ggctgccagg gcggcgataa atgcctgacc cacgcgctgt ggcgtgattt gagcgaccgt
    11161 ctcaccggtt ttctcaacaa cattacttta ggcgaactgg ttaataacca ggaagtgctg
    11221 gatgtgtctg gtcgtcagca tactcacgac gcgccacgca cccgcacaca agacgcgatc
    11281 gacgttaagt tacgcgctta ataaaaagaa ttcagaatca ggccggagtg ctaaatactc
    11341 cgtaaacacg gtcgtacatc cagccggtag cctgattcct tgcattgagt gatgtacgga
    11401 gtttatagag caatgaaatt accgatttat ctcgactact ccgcaaccac gccggtggac
    11461 ccgcgtgttg ccgagaaaat gatgcagttc atgacgatgg acggaacctt tggtaacccg
    11521 gcctctcgtt ctcaccgttt cggctggcag gctgaagaag cggtagatat cgcccgtaat
    11581 cagattgccg atctggtcgg cgctgatccg cgtgaaatcg tctttacctc tggtgcaacc
    11641 gaatctgaca acctggcgat caaaggtgca gccaactttt atcagaaaaa aggcaagcac
    11701 atcatcacta gcaaaaccga acacaaagcg gtactggata cctgccgtca gctggagcgc
    11761 gaaggttttg aagtcaccta cctggcaccg cagcgtaacg gcattatcga cctgaaagaa
    11821 cttgaagcag cgatgcgtga cgacaccatc cttgtttcca tcatgcacgt aaataacgaa
    11881 atcggcgtgg tgcaggatat cgcggctatc ggcgaaatgt gccgtgctcg tggcattatc
    11941 tatcacgttg atgcaaccca gagcgtgggc aaactgccta tcgacctgag ccagttgaaa
    12001 gttgacctga tgtctttctc cggtcacaaa atctatggcc cgaaaggtat cggtgcgctg
    12061 tatgtacgtc gtaaaccgcg cgtacgcatc gaagcgcaaa tgcacggcgg cggtcacgag
    12121 cgcggtatgc gttccggcac tctgcctgtt caccagatcg tcggaatggg cgaggcctat
    12181 cgcatcgcaa aagaagagat ggcgaccgag atggaacgtc tgcgcggcct gcgtaaccgt
    12241 ctgtggaacg gcatcaaaga tatcgaagaa gtttacctga acggtgacct ggaacacggt
    12301 gcgccgaaca ttctcaacgt cagcttcaac tacgttgaag gtgagtcgct gattatggcg
    12361 ctgaaagacc tcgcagtttc ttcaggttcc gcctgtacgt cagcaagcct cgaaccgtcc
    12421 tacgtgctgc gcgcgctggg gctgaacgac gagctggcac atagctctat ccgtttctct
    12481 ttaggtcgtt ttactactga agaagagatc gactacacca tcgagttagt tcgtaaatcc
    12541 atcggtcgtc tgcgtgacct ttctccgctg tgggaaatgt acaagcaggg cgtggatctg
    12601 aacagcatcg aatgggctca tcattaatcg gtatcggaat caggagaatt tataatggct
    12661 tacagcgaaa aagttatcga ccattacgag aatccgcgta acgtgggttc ctttgacaac
    12721 aacgacgaga acgtcggcag cggcatggtg ggtgcaccgg cctgtggcga cgtgatgaag
    12781 ttgcagatta aagtcaacga tgaaggtatc attgaagacg cgcgttttaa aacttacggc
    12841 tgcggttctg ctatcgcttc cagctccctg gtcaccgaat gggtgaaagg gaagtctctc
    12901 gtcgaagcgc aggcgatcaa aaacaccgat attgctgaag aacttgaact gccgccggtg
    12961 aaaattcact gttctattct ggcagaagac gcgatcaaag ccgccattgc ggactataaa
    13021 agcaaacgtg aagcaaaata agagttgagg tttggttatg tcgattacac tgagcgacag
    13081 tgcagcagcg cgagtaaata cctttctggc taaccgcggc aaagggtttg gcctgcgtct
    13141 gggcgtgaga acctccggat gttcaggtat ggcttatgta ctggaatttg ttgacgaacc
    13201 gacgccggaa gacatcgtgt ttgaagacaa aggcgtgaaa gtcgtggtcg atggcaaaag
    13261 cctgcaattt ctggacggta cgcagctgga cttcgtaaaa gaaggcctga atgaagggtt
    13321 taaattcacc aacccgaacg ttaaagatga gtgtggttgc ggcgaaagct tccacgtttg
    13381 atgcgcatac cgataacccc accgtggtcg cctgcgcgtg gggtttgttt tacctgattc
    13441 gccgttatcg cggcggatcg cagccctgag aatgttatgg attacttcac cctctttggc
    13501 ttgcctgccc gttatcaact cgatacccag gcactgagcc tgcgttttca ggatctacaa
    13561 cgtcagtatc atcctgataa attcgccagc ggaagccagg cggaacaact cgccgccgta
    13621 cagcaatctg caaccattaa ccaggcctgg caaacgctgc gtcatccgtt aatgcgcgcg
    13681 gaatatttgc tttctttgca cggttttgat ctcgccagcg agcagcatac tgtgcgcgac
    13741 accgcgttcc tgatggaaca gctggagctg cgcgaagaac tggacgagat cgaacaggcg
    13801 aaagatgaag cgcggctgga aagctttatc aaacgtgtga aaaagatgtt tgatacccgc
    13861 catcagttga tggttgaaca gttagacaac gagacgtggg acgcggcggc tgataccgtg
    13921 cgtaagctgc gttttctcga taaactgcga agcagtgccg aacaactcga agaaaaactg
    13981 ctcgattttt aatttctgga agctaaacat ggccttatta caaattagtg aacctggttt
    14041 gagtgctgcg ccgcatcagc gtcgtctggc ggccggtatt gacctgggca caaccaactc
    14101 gctggtagcg acagtgcgca gcggtcaggc cgaaacgtta gccgaccatg aaggccgtca
    14161 cctgctgcca tctgttgttc actatcaaca gcaagggcat tcggtgggtt atgacgcgcg
    14221 tactaacgca gcgctcgata ccgccaacac cattagttct gttaaacgcc tgatgggacg
    14281 ctcgctggct gatatccagc aacgctatcc gcatttgcct tatcaattcc aggccagcga
    14341 aaacggcctg ccaatgattg aaacggcggc ggggctgctg aacccggtgc gcgtttctgc
    14401 ggacatcctc aaagcactgg cggcgcgggc aactgaagcc ctggcaggcg agctggatgg
    14461 tgtcgttatc accgttccgg cgtactttga cgatgcccag cgtcagggca ccaaagacgc
    14521 ggcgcgtctg gcggggttgc atgttctgcg cttacttaac gaaccgaccg ctgcggctat
    14581 cgcctacggg ctggattccg gtcaggaagg cgtgatcgcc gtttatgacc tcggtggcgg
    14641 gacgtttgat atttccattc tgcgcttaag tcgcggcgtg tttgaagtgc tggcaaccgg
    14701 cggtgattcc gcgctcggcg gcgatgattt cgaccatctg ctggcggatt acattcgcga
    14761 gcaggcggac attcctgatc gtagcgataa ccgcgttcag cgtgaactgc tggatgccgc
    14821 cattgcagcc aaaatcgcgc tgagcgatgc ggactccgtg accgttaacg ttgcgggctg
    14881 gcagggcgaa atcagccgtg aacaattcaa tgaactgatc gcgccactgg taaaacgaac
    14941 cttactggct tgtcgtcgtg cgctgaaaga cgcgggcgta gaagctgatg aagtgctgga
    15001 agtggtgatg gttggcggtt ctactcgcgt gccgctggtg cgtgaacggg taggcgaatt
    15061 tttcggtcgt ccaccgctga cttccatcga cccggataaa gtcgtcgcta ttggcgcggc
    15121 gattcaggcg gatattctgg tgggtaacaa gccggacagc gaaatgctgc tgcttgatgt
    15181 gatcccactg tcgctgggcc tcgaaacgat gggcggcctg gtggagaaag tgattccgcg
    15241 taataccact attccggtgg cccgcgctca ggatttcacc acctttaaag atggtcagac
    15301 ggcgatgtct atccatgtaa tgcagggtga gcgcgaactg gtgcaggact gccgctcact
    15361 ggcgcgtttt gcgctgcgtg gtattccggc gctaccggct ggcggtgcgc atattcgcgt
    15421 gacgttccag gtcgatgccg acggtctttt gagcgtgacg gcgatggaga aatccaccgg
    15481 cgttgaggcg tctattcagg tcaaaccttc ttacggtctg accgatagcg aaatcgcttc
    15541 gatgatcaaa gattcaatga gctatgccga gcaggacgta aaagcccgta tgctggcaga
    15601 acaaaaagta gaagcggcgc gtgtgctgga aagtctgcac ggcgcgctgg ctgctgatgc
    15661 cgcgctgtta agcgccgcag agcgtcaggt cattgacaat gctgccgctc acctgagtga
    15721 agtggctcag ggcgatgatg ttgacgccat cgaacaagcg attaaaaacg tagacaaaca
    15781 aacccaggat ttcgccgctc gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca
    15841 ttccgtggac gaggtttaat atgccaaaga ttgttatttt gcctcatcag gacctctgcc
    15901 ctgatggcgc tgttctggaa gctaatagcg gtgaaaccat tctcgacgca gcgctgcgta
    15961 acggtatcga gattgaacac gcctgtgaaa aatcctgtgc ttgcaccacc tgccactgca
    16021 tcgttcgtga aggttttgac tcgctgccag aaagctcaga gcaggaagac gacatgctgg
    16081 acaaagcctg gggactggag cccgaaagcc gtttaagctg ccaggcacgc gttaccgacg
    16141 aagatttagt agtcgaaatc ccgcgttaca ctatcaacca tgcgcgtgag cattaacaga
    16201 ggttagtatg ggacttaagt ggaccgatag ccgcgaaatt ggcgaagcac tgtacgatgc
    16261 gtatcctgat cttgatccga aaacggttcg attcaccgat atgcatcagt ggatttgcga
    16321 tctggaagat ttcgacgacg acccgcaggc atccaacgag aaaatcctcg aagcgatttt
    16381 gttagtctgg ctggacgagg ccgaataatt tccgcgtcat gcttcacgcc gcagatgcgt
    16441 tggctgcgct cgttcacccc agtcacatag ttatctatgc tcctggggct tcactcactt
    16501 gccgccttcc tgcaacgcga atcatttagc ggaaaaatcc tggggctgcc aactggcggc
    16561 ccttttacaa agaaggataa ctaaaatgac agaagcgatg aagattaccc tctctaccca
    16621 acctgccgac gcgcgctggg gagaaaaagc aacttacagc attaataatg acggcattac
    16681 cctgcatttg aacggggcag acgatctggg gctgatccag cgtgcggcgc gcaagattga
    16741 cggtctgggc atcaagcatg ttcagttaag cggtgaaggc tgggatgcgg atcgctgctg
    16801 ggcattctgg caaggttaca aagccccgaa aggcacgcgt aaagtggagt ggccggatct
    16861 ggacgatgcc cagcgccagg aactggataa ccgcctgatg atcatcgact gggtgcgtga
    16921 caccatcaac gcaccggcag aagaattggg accatcgcaa ctggcacagc gtgctgttga
    16981 tctgatcagc aacgtcgcgg gcgatcgtgt gacttatcgg atcaccaaag gcgaagatct
    17041 gcgtgagcaa ggttatatgg ggctgcacac cgtcggacgc gcttcagaac gttctccggt
    17101 attgctggcg ctggattaca acccaactgg cgataaagaa gcgccagtgt acgcgtgcct
    17161 ggtaggtaaa ggtatcactt ttgactccgg cggctacagc atcaaacaga ctgcgtttat
    17221 ggactcgatg aagtcggaca tgggcggcgc ggcaacggtt accggggcgc tggcatttgc
    17281 cattacgcgc ggactgaaca agcgcgtgaa gctgttcctc tgctgtgcgg ataacctgat
    17341 tagcggcaat gcgttcaagc tgggcgatat catcacctat cgcaacggta aaaaagttga
    17401 agtgatgaac actgatgccg aagggcgtct ggtgcttgcc gatggtctga ttgatgccag
    17461 tgcgcagaaa ccggaaatga tcattgatgc ggcgaccctc accggggcgg cgaaaactgc
    17521 gctgggtaat gattatcacg cgctgttcag ttttgacgat gcgcttgccg gtcgtttgct
    17581 ggcgagtgcc tcacaagaga acgaaccatt ctggcgtctg ccgctggcgg aattccaccg
    17641 cagccagctg ccgtctaact ttgccgaact gaacaatacc ggaagcgcgg cgtatccggc
    17701 aggcgcgagc acggcggcgg gcttcctgtc gcactttgtt gagaactatc agcaaggctg
    17761 gctgcatatc gactgctcgg cgacttaccg taaagcgccg gttgaacagt ggtctgcggg
    17821 cgctacggga cttggtgtgc gcacgatagc taatctgtta acggcgtaaa ctaatatgcc
    17881 ggatgcggcg taaacgcctt atccggccta caaattctgc aaattcaaca aattgcgaat
    17941 cccttgtagg cctgataagc gtagcgcatc aggcatttgc gccccgaacg ttgaatgaat
    18001 tgtggttctt atgtccgaaa caaaaaacga acttgaagac ctgctggaaa aagcagcaac
    18061 cgaaccggca caccgcccgg cctttttccg tactctactg gaatccaccg tctgggtgcc
    18121 tggtacggcg gcgcagggcg aggctgtggt tgaagatagt gcgcttgatt tacagcactg
    18181 ggaaaaagaa gacggcacca gcgtcattcc ttttttcacc tcgttagaag cgcttcaaca
    18241 ggcggttgaa gacgaacagg catttgtcgt aatgcccgtt cgcacattgt ttgagatgac
    18301 ccttggcgaa acgctcttcc ttaatgccaa actgccgacc ggtaaagaat ttatgccgcg
    18361 tgaaatcagt ttgttgattg gtgaagaggg aaatccgctg agcagccagg aaatcctgga
    18421 aggcggtgaa tcgctgatat tatcggaagt tgcagagccg ccagcacaaa tgattgattc
    18481 actcaccacc ttatttaaaa ccattaagcc ggtgaagcgt gcttttattt gttcaattaa
    18541 agagaacgaa gaggcacagc ctaatttatt tattggcatt gaagccgatg gtgatatcga
    18601 agaaattatt caggcgacgg gaagtgtagc gaccgataca ttacctggcg atgaaccaat
    18661 cgatatttgt caggtgaaaa aaggggaaaa aggaattagc cactttatta ccgaacatat
    18721 tgtgccattc tatgaacgtc gctggggagg ttttttgcgt gactttaaac aaaaccggat
    18781 aatctaaaat atgccgccct aaagggcggc attcgggctt acttgctgct ctgaaggttg
    18841 ttaaccacca gtaaattgtc gccggagaga ctccggttat tatttacttg ggaaaacagc
    18901 gcaccagaaa caataagtac gatacagaag caccgtatct tcattgtgcg gtccatgcga
    18961 agaccgcatc acgacagcac cactggcgat attgccgtta gagcaagcgc tgagacgcgc
    19021 ttgctctaac ggtcgcggaa catcctttcc ttaagccagc gcccgcttgc gagacggcgg
    19081 aaagggccgc agtggataat atactaacag aacaataata actctctttt gcttgacaaa
    19141 aagagagtta ctggcgagta ttgtctccgc tgagacgcgc ttgcattact acggtaatac
    19201 aacctgctgg aaaggccgcg aaccagacca gcaataaaaa accgccaaat tcggcggttt
    19261 tttattgcct gtaaaatact tatttcactg gctcaaccgg taaatctgcc cgcgcgcccc
    19321 attcactcca tgcgccgtcg tacagtttca cgtttggcac atccagcgtc gcgagtgcta
    19381 acaaaaccac ggctgccgtt acaccagagc cgcagctgac gataattggt ttgtcgtagc
    19441 tgacgccgcg accaaaaaat atcgcatcca gttcatcggt cgtttttagt tcgccttcgc
    19501 gcaccagttc cgtccacggc acattcagtg ctccgggaat atgtccgcga cgtaaacctg
    19561 ggcgaggttc atcaacttct gcgttaaaac gtgcagccgg gcgggcatca ataatttgcg
    19621 ccgtattttc atggcttgcc aataatacat cggttacttt caccacggct tcaggattaa
    19681 acgcggcgtt aaactctcct tccggcagct ctactgcacc ttcttctaac agcagatcat
    19741 cgcgctgcca gcctgcaagt ccacccccca gaatcgacac tttctctaca ccaaaggtgc
    19801 gcagcatcca ccatgctcgt ggggctgaga aaagattacc ttcgtcatag acaatcaggt
    19861 gcttatcct
//
LOCUS       DAJTYD010000062        19498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTYD010000062 DAJTYD010000000
VERSION     DAJTYD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.062"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..4015)
                     /gene="mukB"
                     /locus_tag="OO585_001995"
     CDS             complement(<1..4015)
                     /gene="mukB"
                     /locus_tag="OO585_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6629593.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELL"
     gene            complement(4015..4719)
                     /gene="mukE"
                     /locus_tag="OO585_001996"
     CDS             complement(4015..4719)
                     /gene="mukE"
                     /locus_tag="OO585_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6629594.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(4700..6022)
                     /gene="mukF"
                     /locus_tag="OO585_001997"
     CDS             complement(4700..6022)
                     /gene="mukF"
                     /locus_tag="OO585_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6629595.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6019..6804)
                     /gene="cmoM"
                     /locus_tag="OO585_001998"
     CDS             complement(6019..6804)
                     /gene="cmoM"
                     /locus_tag="OO585_001998"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6629596.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            6940..7719
                     /gene="elyC"
                     /locus_tag="OO585_001999"
     CDS             6940..7719
                     /gene="elyC"
                     /locus_tag="OO585_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6629597.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7696..8589)
                     /locus_tag="OO585_002000"
     CDS             complement(7696..8589)
                     /locus_tag="OO585_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6629598.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQEHFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(8743..9489)
                     /gene="kdsB"
                     /locus_tag="OO585_002001"
     CDS             complement(8743..9489)
                     /gene="kdsB"
                     /locus_tag="OO585_002001"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6629599.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9486..9668)
                     /gene="ycaR"
                     /locus_tag="OO585_002002"
     CDS             complement(9486..9668)
                     /gene="ycaR"
                     /locus_tag="OO585_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6629600.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(9720..10952)
                     /locus_tag="OO585_002003"
     CDS             complement(9720..10952)
                     /locus_tag="OO585_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6629601.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLALEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(10989..11975)
                     /gene="lpxK"
                     /locus_tag="OO585_002004"
     CDS             complement(10989..11975)
                     /gene="lpxK"
                     /locus_tag="OO585_002004"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6629602.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(11972..13720)
                     /gene="msbA"
                     /locus_tag="OO585_002005"
     CDS             complement(11972..13720)
                     /gene="msbA"
                     /locus_tag="OO585_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6629603.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(13757..16029)
                     /locus_tag="OO585_002006"
                     /pseudo
     CDS             complement(13757..16029)
                     /locus_tag="OO585_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16236..16520)
                     /gene="ihfB"
                     /locus_tag="OO585_002007"
     CDS             complement(16236..16520)
                     /gene="ihfB"
                     /locus_tag="OO585_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6629604.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16680..18353)
                     /gene="rpsA"
                     /locus_tag="OO585_002008"
     CDS             complement(16680..18353)
                     /gene="rpsA"
                     /locus_tag="OO585_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6629605.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18464..19147)
                     /gene="cmk"
                     /locus_tag="OO585_002009"
     CDS             complement(18464..19147)
                     /gene="cmk"
                     /locus_tag="OO585_002009"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6629606.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19320..19498)
                     /locus_tag="OO585_002010"
                     /pseudo
     CDS             complement(19320..19498)
                     /locus_tag="OO585_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
BASE COUNT         4678 a         5409 c         4756 g         4655 t
ORIGIN      
        1 ccagcagttc ttcgccaatg gtctgcggcg tgcgctgccc catatcaatc tgcggattaa
       61 gacgctgata caatttcgcc aacgcttccg agaaggtcag acggttgctg ttaaacagat
      121 cctgatgctg ctcgtgctgt tcagagagca catccagcag catggcgtgc gtttcacgca
      181 cgttcacgtt gaggcgcacg ctgttcacct gaccaaacga tacgttctgc aacccctggt
      241 tgagcatacg gatacggttc tgctcgcgct gaatggtttt gcgaatgatg ttcgccacgc
      301 tgcgggaact gatcaccagt ttctgttcac gggaggttaa ttcttcggtc agacggctaa
      361 gttcgatctc catctgttcg atagcttcca ccggatcatc ggtacgaata atatcctgac
      421 gaatacgttc acgcagatgc tgataagccg ccacgaagaa ctgaatttta cgctccggac
      481 gtttcggatc ttccgacatg cgcagcacgt cgcgcagatg ttcgttatcc gccaccgcca
      541 gacgcagcgc acccaatgcc ttatccgaca tagagcgcaa atcatcagcg gagagataag
      601 ccagctcacg acggtgtaag cggcgctcaa cgccgttatc tttcaccatg cgcatcaccg
      661 cacaccagcc cgctttggcg gtcactacct gctcgcgcat ctcaaagtaa tcccgctcca
      721 gcttgcgcag tttgcgggtc aggttgtcca tctccgcttc gcagaaggta agcgcttttt
      781 ccagttgatt gcggcgtgaa cggttattgc tcagttgcgc atgcagctcg tcacggcgaa
      841 tacgcgcccg ctcttctgcc ccgctatcag cacacacgcc gatatcctgc aattcacgtt
      901 gcagatcgtt gagtagctct tttttggtgt cgtaagaact tttcagcgaa gccagcacct
      961 ggttgtactg actcaactgc gctgcgtgac cgcgcaacgc ttcgcgagcg cgggtacgct
     1021 ccgcttccgc ctgttccaga cgttcacgca gtttttcgtt gagatcgctg ttaccgctaa
     1081 gcatttctgc cgagtcagag tagctaaagt gcgcacgacg ctgcaccact tccgtcaggg
     1141 caaacgcctg ctgacgggca tcgcgctgca tctgttgaga gtacgcgtaa tcttctttta
     1201 actgttcgaa ctgttccggg tcgctctgca ataccgaaac gatcggttcc agtttcgcca
     1261 gttgattgcc aaactgctga acaaaacgcg cggcttcctg ggcttcatcc agacgttcgc
     1321 ggatttcatc gacgcgatcc gccaggctat catcagccaa caggttgaga cgcggcagaa
     1381 tgcggttcag cgccgtaacg ccctctttcg cctgctcaaa ctgaatacgc tgctgctggt
     1441 tatcattttc atgattactt aacgcccgct ccagttcgac gcgacggctg ttcagttgac
     1501 ggatttctgc ttccgggtca gactcaaaca caaccgccag atgactgccg ataaagcggc
     1561 tgaacgcctg atgcagacgc tgagttttct gtacatcaaa ggagagcgtg gcgaagcgtt
     1621 cggaaagcac ttcacgctcg gcatggaggc tttcaatacg gctttcacgc gcagcacgac
     1681 caaacagcgg cacttccggg aaacgtgaat aacgccactg acgatcggcg attttcacca
     1741 ctaccgcttt ttccagctca tcaacgctga acacgctgtc atcgaatgac tgcggatctc
     1801 cttcgatcag atagagatct tccgggcaat cggtcaagcc atctaggtgt tcagttacct
     1861 gtgacagatc tggcaccacg atggcgtggc gtgacgggcc atacagcgct gagaagtacg
     1921 gcgcatcttc caggctaacg tcgtcataaa tttctgacag cagcacacca ccaaaacgct
     1981 ccgccagcgc gttcagacgc tgatcttcag agccgccagg ctggcttaaa cgttcgatct
     2041 cttcatcgac ggcgtttttg cgcgcgccca cttcatcgcg ttcaacaatc gcctctcgct
     2101 cacgctccag caactgttgc agatattcgg tgacgtcctg gctggaggta aactcttcgc
     2161 cgcactgttc gctcaactgg ttgagactgt tttgcgctgc cagccaaacc ggcgcacgct
     2221 gcatcaaact ctgaatgcga gactgcagct gttcctgctc ctggcgcagt gccatgcgct
     2281 cttcacgggc gttagacacg ctatcggaaa gagaggcaat gcgtgcttcc agttcctgat
     2341 gcagggcttc cagttcgtcg atatcaaaat tcttgccctg acgtttgcag aaatctgcca
     2401 gcagacgctc agcttcttgc tgctcgcgta gacgctgttc cagttcgctt aatcgcatcc
     2461 gcagcggctg aacctgctct gccaggtgac gctgatcgac cccttcgcgc aatagttcgc
     2521 gagcgacatc ccacgcctcg ttacgcgcca gtgggccgtt gattgccacc accagctgat
     2581 aagcctgctc aaactggctg tgcgcggttt gcgccatgct cattttctgc tcaagagaga
     2641 gcattttttc agtcgcttcc agctctttcg cctggaaggt ttccagccat tcggcggcgc
     2701 tgtcggcggt taagtccggc agatggcaca gttctttggc acgattaagc gcagcaatcg
     2761 cctggttata ctggatcgcg cgcgtttgct ggacgtccag cgcctgctgg tagtcagcaa
     2821 gctggctttt cagctcgtcc acttccagtt cggcagcttc cgcgcgagcc tcgttctctt
     2881 cctggcgttc gatggcttct gccaccactt cgttttgctc ttccagacgg atctgcagct
     2941 catcgagatc cgcttcgtag cgttcgatct tctcctgctg acgcagtgcg gtttgcacca
     3001 ggttcaggtg atcgctggcc gcctgataat ccgcttccag atcgccttcg gcaccgttgt
     3061 gctctgccag ctcacgcgcc atatcgacgt gtttgtactg ctcagccgcc agttgctgac
     3121 gcgaagtatg taactcgcga cgaaactcca gggctttgtc gagatggaca cggcgctcgt
     3181 tggcgtgacg catgtagtcc gccgccacgt agttggtagc ttcgctgatc agatgcttaa
     3241 acaggtcacg gtccgactgg gtgacacgaa tcgcttccag cgtcatgcgg ttttcacgca
     3301 gcgccgcttc catgtcctgg aacgctttac gcacgccgct gttttccggc aacagatagt
     3361 cgcgcagaga acgggtaatg gcactggaga tcccgccata cagcgaagct tcgatcagac
     3421 gatagaattt gctacggtca gatgcggagc gcagacgacg cgcgatgatg cccagatcga
     3481 acatcagcga gtggtaatcg gtaatggagt tgaactgttt aaactgcacg ccttccatcg
     3541 cctcgagctt gtctttcagc tcgttaagcg gcagcacgcg cgcctggcgt tcgttcaggg
     3601 tttcggtcac cagctgtgtc ggctgcaccg acatcggcag tccctgaatg gcaaatggtt
     3661 tgatatccac tttacgatcg cgtccggcaa cctgttgcag acgcacaccg accaccacgc
     3721 gctggtggcg cgagttaatg gtgtcgagca tcgaataaca gacacccgct ttcagcttac
     3781 cgtgcagacc tttatcgcgc gaaccgctgg tggccccggc ttccgtagtg ttacggaaat
     3841 gcagcagggt caggtcgggg atcagcgccg taacgaacgc cgccatggtg gtggatttac
     3901 ccgccccgtt accgccagaa agcgtcgtga ccagctcgtc aaggtcaaaa gttcgggcaa
     3961 aaaagccgtt ccagttaatc agcgtcagtg agcgaaattt accgcgttca atcattattc
     4021 ttcctctccg ctatctggct gattctcttc ggtttcatcg ttgagttgca gatgattttc
     4081 aatcggcatt gcttcgccat cacgaatcag gcgacgctgt gcttcacggg gatcgtcgcc
     4141 agcacgcaca tcggctccga agcggaacac cgattcggta atgcgaaact tgctgctgtc
     4201 gtggcccata aaccacacca tgcctaaacg acgcagacgg ttgagcgaag aacgtacttt
     4261 ctcctgcaac ttctgacggt caacgtctga accggttgaa cggttgttca ccagtttcag
     4321 cagttttgct tcatcggcca gggtgagcag ttcgtcgtac agttcctgct gggtgaaaat
     4381 cccctcattc gccagccgtt ccgggctgag atagagataa cagaggattt tcccgaccat
     4441 catatccagt tccgacaaga cggaacgcgg gataagagtg gtggaacgtg ggcgtaaata
     4501 gaagaaccct tccggtgcgc gaataagctc aacgttataa cgcgcgtaaa actcttccag
     4561 atattcctga aaatccatca ggaatgcatg attatccagt tcgtcgaggc caatatggcg
     4621 gcctgaacgt aaggcgctgt ccagcgccgg aaataacgga ttcgccagcg cctgcgccag
     4681 cttaaccggc atcacttgtt caatatttgt cgatgacatg cgcctgtacc ttggctccgt
     4741 aatcattaat cggctgccat ttcgctggca gtccggtgaa atctgcttgc gctacgccaa
     4801 gacgtaccgc ctgatcaata acaatacgcg caacgtcaaa gtgacgtgca cgcggatact
     4861 gtgacagata ttcgcgtacc accagaccaa gatccagcgg cacttgtctg gttttgtaca
     4921 cggcaagttg ttcttcgatg atcgccgcca gctgttcgcg gatctcgtta aactcttcgt
     4981 attccagatc ctcaggaagt tccccagtca cttcttcatc gcgcagtgcc atctcttcgt
     5041 cacgcatatc cagcagacga tcggcattgg cataagttag cgcccacggc tcatcaaaat
     5101 aggtttgtac cgactgacgt aaccgctgag caaagacgcg gtttttatcc atatcgatcg
     5161 cggtacgaat aaatttgtgt acgtggcggt cgtagccaat ccacaagtcg atggattgct
     5221 ggccccaact gataatacga tcgagtttgc tctgcagatc gaacaccaga cgatcgacga
     5281 aatgcagatc gtcatgggtc atcgtcgcat cctgaatgcg caacagatta gcctgcaatt
     5341 tgtcgcctgc cgcttccagc gtatcctgca attcacgcag cgttccggaa gtttccgaaa
     5401 gcaacaattc acagctggaa atcgccgccc gccagtcttt gttcagcaac tgggcgatat
     5461 cgtccttcac ctgctgctgc tgttcgtcca tcagacgttg cgtcaggtcg atactgtcga
     5521 aaatttctgc taccgaatat ttcagtggcg catagacatt acggtgccag tgaaattcat
     5581 caccgccctc ttcggcggca tctgctgcgc gtttgagctc acccgccaca atcgacaact
     5641 gcatagaaag acgcagcgta gaaaactcgc gctgacggat gtagtagtca gtaatgccga
     5701 tgccgagcgg cgtcagacgg taaattgcgt tcccttccgc ctgctcgctg gtaaagcggt
     5761 tcagcagacg ttgacgcacc atgtcgttga tcgcgttatt ggcgcgcacg ccgatggttt
     5821 cgctggtttg ctcaaacgca tcactcacat ggcggaatgc atccaccagc tcgccttcac
     5881 tcatctcacc atccagacgc tcgccgttca gcgtggcaac cgccagcaga aaagagagtc
     5941 ggtctacctg cagcgagatg gagaagtcat tttttctggc ccaggcaacc agttcgggga
     6001 ctgtctggga aaattcactc atactttatc cttgctctgc ggtttgcgcg cggtgacatg
     6061 aatataacgc cccagggtaa tatacggttc ctgacggcaa taacgcgttt ctaattcaag
     6121 taatgcttca tagcagtcgc gctgctggtg cttctcgcgc agataatcat gaaacacgcg
     6181 aacgcctgtc ttacccataa tttgccaacc agcttcttcc agccacagat aaacctgcgc
     6241 cgggtcgcgt ggataatctg gcgaaagcgt ccgttttttc tttttcggca ttcccgcctg
     6301 cacgtaatca aaattcccgg cgaccatgtt atgcatcaac aaaccatgcg cattgtagaa
     6361 cattaacgac aacacgccgc ctggacgcaa cactgaccac agggtctgca atacactgcg
     6421 gggatcggcc acccactcga gcaccgcatg gaacaatatc agatcaacgg gcgtttccaa
     6481 atgcgaagca acatcctgag cggcgcaatg tataaattgc atgttgtcgc tcacaccttt
     6541 tgcttctgcc gcctgttttg cgcggtcgat catctgcgca gaaagatcgc ataaaatgac
     6601 ctgatgccca cgctcggcca ttttgattgc ggtctgccct tctccaccgc cagcatccag
     6661 cacacgcagt ttttgcgggc ccatttccgc cagcacgcga tcgagatcct gccacagaat
     6721 agcctgtcga agctgccctt tggtggtgcc gtaaatgtta cgggaaaact tttccgcaat
     6781 atcatcaaaa ttgcgatcct gcattgcttt ctccaccggt tggctaagac gttttctgat
     6841 aaagacgcta ttttgtcaca gccacggcga gaatgaaccc gtctggtgta atatcgctgg
     6901 cgatcccttg ctatatggtt aaaaaaggaa ccagaaagga tgctttttac actgaaaaaa
     6961 gtgattggca atatgttgct gccacttccg ctgatgttgt taattattgg cgctggcctg
     7021 gcgctgctgt ggtttagccg ttttcaaaaa accgcgaaga tttttattag cattgggtgg
     7081 ctggcactct tgttattgag tttgcaaccg gtagccgacc gcctgctgcg tcccatcgaa
     7141 agcacctatc cgacatggaa taattcgcaa aaagtggact atatcgtggt gctcggaggt
     7201 ggttatacct ggaacccgca gtgggcaccg agctctaatt taatcaataa cagtctgcca
     7261 cgcctgaatg aaggtattcg cttatggcgc gaaaatccgg gatcaaaact gatcttcacg
     7321 ggaggcgtag caaaaaccaa tacggtgagt acagcggaag taggtgccag agttgcgcaa
     7381 tcgctgggcg tgccgcgcga gcaaattatc accctggatt tgccaaaaga taccgaagaa
     7441 gaagctgcgg cagtgaaaca ggcgattggt gacgcccctt tcctgctggt gacctccgca
     7501 tcacatctgc cgcgggcgat gatctttttt cagcaggaag ggttaaatcc tctaccagcc
     7561 ccggcgaatc agttggccat cgactcgccg cttaatccgt gggaacgggc aatcccttct
     7621 ccggtctggc tgatgcatag cgatcgtgtt ggctatgaaa cgcttggccg catctggcag
     7681 tggctgaaag gtccgtcagg cgagccacgg caagagtgat tttgatgcca gatcgaagtt
     7741 gcgccgacta aatcgtccgg tattaaccag ttgcgcaact tcatcccata acacatacaa
     7801 ccaacgccgc cagatgaacg actccgccac tggcgcgcgt tgcaggtaac tccagagcaa
     7861 atcttccgcc agagaattat ccattagtcg gaacagttcg tattctcgcg gtgcccaaag
     7921 cattagtccc ggcccgacca tcgccagtaa ctgatcgctg cgcgaatctt tcagcatgct
     7981 gcgtaaacag aagttaccgt gaatcaacac acaattgtcg ttaaagcctt caaataatgc
     8041 cgggagacat tcgcgagtgc gaaacaggat ccgcttatcc tgcatcgtca gaccggtgtt
     8101 attgaactga ttgagcgtgg tccatagcac ttcaacatgt tgccggtacc atgagggcca
     8161 gaaatgttcc tgagtattgt cgaccgcgcc gacgcaaccg cgactgtcct gacggtgcca
     8221 ggccagtaag gcttcaacga tttggtcttt gagctgttcc cagcgttctg gtgttcgggc
     8281 tggtgcctcc accgaaacac cacgcatccg ctccagtagc aggacatcag ggccggggtg
     8341 ttcttcatgc gtcatcacac cataaatagt cggcatacgg acagtcccac ttcttgccag
     8401 catggtggtt ttccatgcca gttgtcgggc ttttccgggc gtactaaagc ttcttgccat
     8461 taacggcatt gggtttccct ggctgtcata caaagcccac aacgccgtat ccgctttttc
     8521 attgacgcac tcaatacgac tgagtttttc gcccagtaaa tggcttaatt cggcacgcag
     8581 ctgttccatt cagattaccc tcataaaagc tactgctttc ataatgagcg ccaatataaa
     8641 agatgtcacc tgaatgagat caattgttga aaagaaaaat tgaggaaaat cgtaacggta
     8701 cgacactcct cccaaaattg gctgaagtgt cgtgaagtga aattagcgca tttcagcgcg
     8761 aacgcgctca agatcttcag gggtatccac acctgtgcca ggaacttcct gagcaacagc
     8821 aacatggatt ttttcaccgt accacagaac acgaagctgc tctaacattt cgatgtgttc
     8881 taacggactt gcctgccagt tgacgtaacg acggataaag cctgcacggt agccataaat
     8941 gccaagatga cgcaggaagt tatcgccaac ggtttcaagg ccttctgcaa aacgatcacg
     9001 atcccaagga atggtggcgc gtgagaagta cagtgcatac ccttcagcgt cgagaaccac
     9061 tttcaccgca ttcgggttaa acgcttcttc cgcattgtgg attggcaccg ccagagtcgc
     9121 catacccacc tgacgctgag cgaggttatc agcaacctga cgaatgattg tcgcagggat
     9181 catcggttca tcaccctgca cattaacgat caccgtgtcg tcgctgaatg cgcatttttc
     9241 gacaacttcc gccagacgtt ctgttcctga ctgatgatcg gcgcgcgtca tacatacttc
     9301 accgccagcg gcttcaacgg cgcgggcaac atcctcatga tcggttgcca cgatgatgcg
     9361 ctcggcacct gattcacgcg cgcgttcaag aacatgaaca atcatgggtt tgccgttaat
     9421 atcaaccaat ggtttaccgg gcagacgcgt ggacgcgtag cgcgcgggaa taatgaccac
     9481 aaaactcatg atttactctc atcagcagtc agcacgcggg cttccgtttc cagcaacact
     9541 ggaatgccat cgcggagcgg gaaagccagg ttatccagtt tgcaaataag ctcttgtttt
     9601 tcctggttat accaaagttt tccgttgcaa accgggcagg caatgatttc aagcaaacga
     9661 tgatccatag ttcctccaga tggatcgggt tatgaatgca taaatcttat cataatcatt
     9721 tatgcgacgg ggtctatttc ccagccagtg cggcaatcag taaagagacc ttgcgggcag
     9781 cgtcctaatg tcacccgcgt tgcctgctgc cagctcgcga aatcagtaat cgcctgacgt
     9841 aacccttttt gcagcattgt cgttggttta atgccttcct gtaaccacag agagataact
     9901 tcaaggatgc ctgtctggcg atgcattttg gcatccattc gcccaactaa ttgcccacga
     9961 tgtaataatg gcagaacaaa atagccatac tggcgtttcg gcgctggggt atagcactcc
    10021 agccggtagc taaaatcaaa aagctgctct gcgcgtttgc gatcccagac aacaggatca
    10081 aaaggcgaaa gtaccgcgct gtgcgttgca gtgagctttc cggccagcgc acgctcgagt
    10141 agcggcagca aatcagcatg cagccaaaga ttgcccaatt tttcaacatg cacagcaatg
    10201 atttgctgct gttcagccct cgcttcgcgc cacgccgcca gtgccggacg tttcagccga
    10261 tagtaatctg ccagccactg ttcgcggaat attcccaggc tacgcgcact gttatccagc
    10321 atgatgattt ctgcttctgt ttgcgaaacg agatcgcgct catcatccca gtcaggcatg
    10381 acacggtggg ttaaatcata aacgcgctgg aagttgcgcc gttcaatcac catcaccttt
    10441 ccggcagtaa ataaaccttc cagatgccgt ttatgcggtt tccattccca ccagccgctt
    10501 gcacctttac gaggatgctc aaaatcggct gaacgtaccg gccccttatc atgaatatgc
    10561 tgaattaact gtgcaatttc cgcctcatgt tcctgcatcc aggcgtcttt gtatttccag
    10621 cccatttttt caagtgccag catgcggtgg cgaataagac gaaagtcgct acgcggcata
    10681 aagcaggctt catgcgccca gtattccatt aattcgccac gcgccagaga ctcatccagc
    10741 cactgggcag gataatttcc cagacgactg aaaagcacca gatatggact acgggcaaca
    10801 atattgatgg tatcgatttg cagcaaggac atgcgggaga tcgttgccgg aatatcctcc
    10861 aacgacgctc gacggcgggg tttgtttaac aggccttgtg cggcaaggtg aagattacgc
    10921 gcatcagcaa gggagaggtg cggcagcgac attcatgact ccatcaatcg aacgctgccg
    10981 cggcgtaact agttgccaga agccagcgag gttagttgcg taagcagttt cgctggttca
    11041 tcacctgaaa gctgtgcgtc tacaggcaaa taccaccaat tttcttctgc aaaggcccgg
    11101 catttcaccg catctttttc agtcattacc agcgtttgcc cggcgcttac caacgcactg
    11161 acatccgcat ggttcaaaga ctgatgatcg gccagcggta cacatttttc cggttgtacg
    11221 ccacacatct tcagcgtggc aaaaaagcgc ggcggatgcc caatccccgc catcgccact
    11281 acatgttcaa gctgagcaac gtcacaacgc gtaccggtac gtaaattcac cgcctgaccc
    11341 ggcagcagat gcatgggaat ttcaccgctg cgagggacac caccgttgac gattaccgca
    11401 tcaaccgact ttaagcgccc cgctcgctca cgcattggcc ccgccggcaa ccaccagcca
    11461 ttgccaaagc gacgcacacc atcaataacg acaatttcca catcacgcgc cagacggtaa
    11521 tgctgtaaac cgtcgtcggt tacgatgatc tgcacatcag ggtgttgcgc cagaatggct
    11581 tttaccgcat cagaacgaac gggagaaacc gcaacaggcg catcagtgcg ttgataaatc
    11641 aacacaggtt catcacccgc ctgtgctgtt gtggtatctg ccgacaataa cagcggataa
    11701 gattcagcct tactaccata tccccgcgat acgaccccca cgcgaatacc gcgctgttgc
    11761 aactgttcca ccagccagac aacgaccggg gtttttccgt tgccgcctgc ggtgagatta
    11821 ccaaccacga caaccggtac gggggcacgc caggcgcgct tcagttttag tttatagcaa
    11881 agacggatcg cgccactcac caggccatac aaccaggaga gtggcagcaa tagccgccac
    11941 aaaggggatt caccagacca gattttttcg atcattggcc aaactgcatt ttgtgaagtt
    12001 gcgcgtaaac gccgcggtgc tcaagcaaat cgttatgcgt accgcgttcc acaatgacac
    12061 catcctcgac gaccacgatt tcgtctgcct tttcaatggt agacaagcgg tgggcaatca
    12121 ccagagaggt acggtttttc tgcaactcat ccagtgccgc ctgaatcgca cgttcggatt
    12181 cggtatccag agccgaggta gcttcatcca gaatcagaat cgggctatca cgcagcaagg
    12241 ctcgagcgat agcaatacgc tgacgctgac cgccagagag cagcacgccg ttttcaccaa
    12301 tcactgtatc gagaccgtta tccatcttat tgatgaagtc catggcgtag gccatacgcg
    12361 ccgcttcttc aatttgctca cggctgtact gttcagtccg tgcgtaagca atgttgttag
    12421 caaccgtatc gttaaacaga tggacattct gcgacaccag agcaacctgg ttacgtaacg
    12481 acgccagggt atactcgcgc agatcgtgac catccatcag gatttcgcct tcatcaatat
    12541 cgtaaaaacg agtgatcagg ctggcgatgg ttgatttacc tgaaccagag cgtccaacca
    12601 gagcaaccgt cttccctgcc ggaattttca ggttgatgtt acgcaatgca ggtacgtcac
    12661 gtcccggata agtaaaggtg acattgcgga attccacgtc gccagtcgca cgctcgatca
    12721 cgcgcttacc ttcgtctttc tcctgctcac tgtccagaat ggtaaacagc gtctgacaag
    12781 ccgccatacc gcgctggaac tgggcgttaa cgttggtcag tgatttcagc ggacgcatca
    12841 gtgcaatcat tgaagagaaa acaacggtaa tcgtaccagc agtcaggcta tccatgacac
    12901 ttgggaagct cgccgcatac agaacaaacg ccagcgccaa agaggcgatc agctgaatga
    12961 tcggatcaga gatggaagag gctgaaacca ttttcatccc ctgaagacgc attcggttgc
    13021 tgactttatc aaagcgtttc gtttccactt cctgaccacc gaaaatcaat acttctttgt
    13081 ggcccttcag catttgttcg gcgctggtgg tcacctgccc catggtgttc tgcatgtttt
    13141 tactgatgtt gcgaaaacgc ttcgatacta cgcgaatcgc aatcgaaaca atcggtgcca
    13201 gcacaatcaa aatgatcgac agttgccaac tgtaatagaa catcatgatg aacaggccga
    13261 tgattgacgc accttcacgc acaaccgtta tcagtgcgcc ggaagaagaa gaagcaacct
    13321 gttcggaatc gtaggtaata cgtgacaaca gcgtccccgt tgactgtttg tcaaaaaatg
    13381 aaaccggcat tcccatcatg tgaccaaaca ggcggcgacg catggtcatt accacctttc
    13441 ctgataccca ggagatacag tagctggaga catagctggt gataccacgt aaaatcatca
    13501 gcccgatcac caccagcggc atccacacca gcacggagcg atctgtttta ccaaagccat
    13561 catcaagaag tggcttaagg agcgataaca tgaaggtatc gctggctgcg ttgaggatta
    13621 acgctacgcc cgccacgatc agacccgctt taaaaggcgc aatggttggc cacagtcggc
    13681 ggaacgtctg ccacgtagag agatctttgt cgttatgcat tcaaaaaacc agcatttgtt
    13741 gaaatagccg catattctac ccgttatcca ctggcacgcc aaaccactga tgataccaac
    13801 gaggtaaaat ttgctctctg aggctgctaa tccgccagcc ttgcgctgaa aaattgaccg
    13861 tcacttgacc ctgatgtgga gtatcaagcc attgatatcc ttgctgttga tagcgatgct
    13921 taactttatt agagggcaat cgccatgcgt tatagcgtta tagcgcgatg ccgatgcgag
    13981 tgccactttt ccattcactc gctgaattaa tggcaacgat gatgaggtat tactgccatg
    14041 gtgaggtacc tgaagcaatg ttgtctgcac ttgctgccag taacggctta gcatcttttg
    14101 ttcagcgggg acttcaatat caccggttag aagaatgcta ttcgtcccgt cataaacctt
    14161 aaccacacag gaatggttat ttcctttatc gttgctagct tgtaaaggcc agtgcacgct
    14221 gaaacgcaat ccttgccatt gccacgcttc gccacgcaca cagggctgat gatgttccca
    14281 gtttaacgga cttctgatcc ataacatcgg ccatgtgtgc aatattgagt ccagccctcc
    14341 ccggtgatcc agatgttcat gactcagaat aacgccttcc ggttcaagat tatgccagtg
    14401 gagccagggg ataatcagtt gttgcccact atccccttcg ggccaggcca gtcctgtgtc
    14461 atagagaatc gctttgccgt ttctggctat caccattgcc agcccttgcc cgacatcaag
    14521 catgtacacc tgccactcgt caggtcgagg tttttgccac agcggccagc acatcagcaa
    14581 gcctacagcc acacacattg ctggcaacgt tcgccaggcg tttaatcgcc ataccaccag
    14641 taagaaccat ggggaaaatg atagccattg ccaacgttca gcaatgttga tccacccttc
    14701 tggcaaactc tttaaccccc agaaaagtaa agccaaagac cggtcggcaa gaaaccataa
    14761 cccttgctcc aggattaacg gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg
    14821 aaccgtgata aatgtcacca agggaattgc taatagattt gcaataaacg aggtcagact
    14881 aatgccatga aatatgacga tttgcacagg cataagcagg agtgtgattc ccagttgcaa
    14941 atgaatgagg gaaacaactg cacgcaatac cggcggcagt tgccactcag gacagggaaa
    15001 ccactggtac caaaaaatca gtgccgcgac cgcagcggca gagagccata aactttgcga
    15061 gagaatggca acaggatcca tcagcaaaat tgccgccaga caacatatcc atacatccca
    15121 gccactccac tgtcgcccac ttaacttaag cattccccac gtagcaagcg ccaccacggt
    15181 acgcaatgca ggaggttgca ttcccgtcaa ccaggcataa aaagcagcac agcagattcc
    15241 accaattaat ggcatttgcc agtggatcca gcgcccaggc agaaaaactt gcccaccgcg
    15301 aatgagtccg gcagccagca acgccgcaaa agcgatgtgc aatcccgata tcgccattaa
    15361 atgcgccgtt ccagtatcac gcatgatatt tttgatttct ttggggacgg ataaccgttc
    15421 ccccatacct aaaccaagaa taaccgcatt ccacatatag ggttgcagcg ttgtttgtag
    15481 tgacgccaga tactgtgcac gcaggctaca attcggttca atgacacttg cctgcagaaa
    15541 gcggccgatg agcggctgat gctgggcaat ggcataacgc tggctatcaa agccgccatc
    15601 attaagttgg ccatgaactg cacgaacttt gagtttcatt gaccattgtt gtccagcaca
    15661 aaccgcttgc ggaagatatt cgccatacag cacaagacct ggcgcaggga atatacgtcg
    15721 accttgtaga tgagtaattt gaccgtaatg agtggtcatg ccgtcagttg cagtgatctc
    15781 aacaattgca tcctgcgtcg cgccagttaa ggtttctcct gcccacaaaa tttgctttgc
    15841 tgataatatg ccccaaacga aaaacagcaa cgtcagcgcg atataacgga cggttttaac
    15901 agggataaat gccagtacgc aggcgaagag agtcagaaac gcaagggtta atgtcccagg
    15961 caattggggc aaaatcagca acggaaaaat tccgcaaatt atgcatacgc cgactgtcgt
    16021 tattttcatg cacccctccc tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg
    16081 gtgggaattt tgcgttcccc gaggcacatt cagaagaatt taacgaaagg gtgaaaactg
    16141 ctcaaaactc tgcgaggcag cttccagttc ctgattagag aaatgaaaaa agcacccgac
    16201 aggtgctttt ctcgcgttca agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg
    16261 cgcagttctt taccaggttt aaagtgagga acgtattttc cttccagttc tactttatcg
    16321 ccagtcttcg gattacgtcc ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa
    16381 ccgcggattt caatacgctc gccctgcgca agagtcgagg ccatatgctc cagcatctct
    16441 tttactgcat cttcaaccgt cttggcggga atgtgcgatt gctgggtggc aagtctttct
    16501 atcaattctg acttggtcat gattcctccg gttccttaaa ggcaaattaa gcggctgctg
    16561 ttgctgcatt gattaagggc ggccgtagcc gcccttagtg cttgattaca ggacgaaacc
    16621 tgcaatctgt caagtaaact caacaaactt cggaataaaa atcccgaaga gtcagagaat
    16681 tactcgcctt tagctgcttt gaaagcttca gccattgcgt tgttggagaa gtttgcatct
    16741 tcctgtttgt taacagttgc gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca
    16801 gacaggctga ttgcgcggtt tttacgatca acgccggtga atttagcttc aacttcgtcg
    16861 ccaacgctca gaaccagggt agcgtcttca acgcggtcac gggatgcttc agaagcacgc
    16921 aggtaacctt caacgccgtc agccagttct acggttgcgc ctttagcgtc aactgcagtt
    16981 actttaccgg ttacgatagc gcctttcttg ttcagagcaa cccagttgtt gaacggatct
    17041 tctgcgagct gtttaacgcc caaggagata cgttcacgtt ctgcgtcaac ctgcagaaca
    17101 actgcagcga tttcgtcgcc ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc
    17161 caggagatgt cagacaggtg aaccaggccg tcgatgccgc cgtctaggcc gatgaagata
    17221 ccgaagtcag tgatagactt gattttacct tcaacacggt cgcccttgtt gtgggtttcc
    17281 gcgaactgct gccacgggtt agctttgcac tgtttcagac ccagggagat acgacgacgt
    17341 tcttcgtcga tatccagaac cataacttcc actacatcgc caacgttaac aactttggac
    17401 gggtggatgt ttttgttggt ccaatccatt tcggaaacgt gtaccaggcc ttcaacgcct
    17461 tcttcgattt caacgaagca gccgtagtcg gtcaggttgg tcacgcgacc agtcagtttg
    17521 gtaccttccg gataacgttt agcgatagct acccacggat cttcgcccaa ctgtttcagg
    17581 cccagggata cacgggtacg ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg
    17641 cccacgttga cgatttcgct cggatgctta acgcgtttcc aggccatgtc agtgatgtgc
    17701 agcaggccgt caacgccgcc cagatcaacg aatgcaccgt agtcagtgag gttcttaacg
    17761 atacctttaa cttccatgcc ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg
    17821 ttttcggatt cgataacggc acgacgagaa acaacaacgt tgttgcgctt ctgatccagc
    17881 ttgattactt taaattcaag ctctttgcct tccaggtgca gagtgtcacg caccggacga
    17941 acgtctacca gagaacctgg caggaacgca cgaataccgt tcagctcaac agtgaagccg
    18001 cccttaactt tgccgttgat aacaccggta acagtttcag catcttcgta agctttttcc
    18061 agcgtgatcc aggcttcgtg acgtttagct ttctcacggg acagcagagt ttcaccgaag
    18121 ccgtcttcta ctgcgtccag agcaacgtca acttcgtcac ctacctggat ttccagctcg
    18181 ccctgggcgt ttttgaactg ctcagccggg atggcggact cagatttcag accagcgtca
    18241 accagtacta cgtctttgtc gatagcaaca acaacgccac gaacgataga acccgggcgg
    18301 gtttcgattt cttttaagga ctcttcaaag agttgagcaa aagattcagt catgtttaat
    18361 cttcaggttt atatttaacg tccacctggc tccatgccgg atggggttgt ttaacatacc
    18421 cgctgtcatt ccattgcaac gggggtactg caaattcggt cgcttatgcg agagccaatt
    18481 tctggcgcgc gtattgtagc gctttttcaa tcacttgctc aatgcttaag gtggtggaat
    18541 ccaacactaa agcatcggct gccggaacca gtggcgctac cgctcggtta cgatcgcggt
    18601 cgtcgcgttc tttgatctcg gccaaaaggc gctcaaagtt aacactaaag cccttctcct
    18661 gcaactgtag catgcggcga tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca
    18721 ctggtgcatc agggaatacc accgttccca tgtcgcggcc atcggcaatc agacctggta
    18781 attcgcgaaa cgcgcgttgg cgacgcaata atgcttcacg aacgcgtggg aatgtcgcga
    18841 cttgtgaagc cgcattcgcc acttcctgag tacgaatttc gccgctgaca tcttcccctt
    18901 cgaggatcac ttccagattg ccattggttg acacaaaacg tacatccaga tgggatgcca
    18961 gcggtaccag cgcatcttct gacgcaacat caacatggtg atgtaatgcc gccaatgcca
    19021 gtacgcgata aattgcaccc gaatccagca gatgccattg caatgcttcc gccatagcct
    19081 tacacaaggt gcctttccct gcaccgcttg ggccatcaat ggtaataacc ggggcaattg
    19141 ccgtcatctt tatctcctta aaacaggcgt accgttaaca taaccgcgcg cattatacgc
    19201 gccgacgcca caaatcgtta acgttgtgtg caaaaaccag ttttgtctgt attcagatgg
    19261 ggatttattg cgcaattata gcgggctggc agaagaccag cccgaaagat gacaatgatt
    19321 tacttaccag aggcgatacg atcacggatg tgttgcgcac gctctgttga tggtgggtga
    19381 gagtcaaaca tggactgggt gcgaccgcca tccagactag ccagtttttc aaagctgcca
    19441 accagcccct aggtgctgat gccacgtttc ttcaacagat caaaggagaa atcatctg
//
LOCUS       DAJTYD010000063        19375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTYD010000063 DAJTYD010000000
VERSION     DAJTYD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.063"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(35..1864)
                     /gene="glmS"
                     /locus_tag="OO585_002011"
     CDS             complement(35..1864)
                     /gene="glmS"
                     /locus_tag="OO585_002011"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6629607.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2026..3396)
                     /gene="glmU"
                     /locus_tag="OO585_002012"
     CDS             complement(2026..3396)
                     /gene="glmU"
                     /locus_tag="OO585_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6629608.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQESNTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3748..4167)
                     /gene="atpC"
                     /locus_tag="OO585_002013"
     CDS             complement(3748..4167)
                     /gene="atpC"
                     /locus_tag="OO585_002013"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6629609.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4188..5570)
                     /gene="atpD"
                     /locus_tag="OO585_002014"
     CDS             complement(4188..5570)
                     /gene="atpD"
                     /locus_tag="OO585_002014"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6629610.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5597..6460)
                     /gene="atpG"
                     /locus_tag="OO585_002015"
     CDS             complement(5597..6460)
                     /gene="atpG"
                     /locus_tag="OO585_002015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6629611.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6511..8052)
                     /gene="atpA"
                     /locus_tag="OO585_002016"
     CDS             complement(6511..8052)
                     /gene="atpA"
                     /locus_tag="OO585_002016"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6629612.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8065..8598)
                     /gene="atpH"
                     /locus_tag="OO585_002017"
     CDS             complement(8065..8598)
                     /gene="atpH"
                     /locus_tag="OO585_002017"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6629613.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8613..9083)
                     /gene="atpF"
                     /locus_tag="OO585_002018"
     CDS             complement(8613..9083)
                     /gene="atpF"
                     /locus_tag="OO585_002018"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6629614.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9145..9384)
                     /gene="atpE"
                     /locus_tag="OO585_002019"
     CDS             complement(9145..9384)
                     /gene="atpE"
                     /locus_tag="OO585_002019"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6629615.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9431..10246)
                     /gene="atpB"
                     /locus_tag="OO585_002020"
     CDS             complement(9431..10246)
                     /gene="atpB"
                     /locus_tag="OO585_002020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6629616.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10255..10635)
                     /gene="atpI"
                     /locus_tag="OO585_002021"
     CDS             complement(10255..10635)
                     /gene="atpI"
                     /locus_tag="OO585_002021"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6629617.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11252..11875)
                     /gene="rsmG"
                     /locus_tag="OO585_002022"
     CDS             complement(11252..11875)
                     /gene="rsmG"
                     /locus_tag="OO585_002022"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6629618.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11939..13828)
                     /gene="mnmG"
                     /locus_tag="OO585_002023"
     CDS             complement(11939..13828)
                     /gene="mnmG"
                     /locus_tag="OO585_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6629619.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14207..14650)
                     /gene="mioC"
                     /locus_tag="OO585_002024"
     CDS             complement(14207..14650)
                     /gene="mioC"
                     /locus_tag="OO585_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6629620.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14740..15198)
                     /gene="asnC"
                     /locus_tag="OO585_002025"
     CDS             complement(14740..15198)
                     /gene="asnC"
                     /locus_tag="OO585_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6629621.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            15350..16342
                     /gene="asnA"
                     /locus_tag="OO585_002026"
     CDS             15350..16342
                     /gene="asnA"
                     /locus_tag="OO585_002026"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6629622.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16347..17798)
                     /gene="viaA"
                     /locus_tag="OO585_002027"
     CDS             complement(16347..17798)
                     /gene="viaA"
                     /locus_tag="OO585_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6629623.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17792..19288)
                     /gene="ravA"
                     /locus_tag="OO585_002028"
     CDS             complement(17792..19288)
                     /gene="ravA"
                     /locus_tag="OO585_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6629624.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARALEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4627 a         5121 c         4856 g         4771 t
ORIGIN      
        1 ccggtcggca gccctttttt atctatgata gtgattactc aaccgtaacc gattttgcca
       61 agttacgcgg ctggtcaacg tcggtgcctt tgatcagcgc gacatggtaa gccagcagct
      121 gcagcggaac ggtgtagaag atcggtgcaa tcacctcttc cacatgcggc atctcgatga
      181 tgtgcatgtt atcgctactt acaaaacccg catcctgatc ggcgaagaca tacaactgac
      241 cgccacgcgc gcgaacttct tcaatgttgg atttcagttt ttccaacagt tcgttgttcg
      301 gtgcaacgac gataaccggc atatccgcgt caatcagcgc cagcgggccg tgtttcagct
      361 cgccagcagc gtaggcttca gcgtgaatgt aggagatctc tttcagcttc aatgcgcctt
      421 ccagtgcgat tgggtactga tcgccacggc ccaggaacag tgcgtgatgt ttgtcagaga
      481 aatcttctgc cagagcttca atgcgtttgt cctgagatag catctgctca atacggctcg
      541 gcagcgcctg cagaccatgc acaatgtcat gttcaatgga ggcatccaga cctttcaggc
      601 gagacagctt cgccaccagc atcaacagca cagttaactg agtggtgaat gctttagtgg
      661 acgccacgcc gatttccgta cccgcgttgg tcatcagcgc cagatcggat tcgcgcacca
      721 gagaagaacc cggaacgtta cagattgcca gcgaaccaag gtaacccagc tctttcgaca
      781 gacgcaggcc cgccagggta tccgcggttt cgccagactg tgacaaggtg atcatcaggc
      841 tgttacgacg cacggcagat ttacgatagc ggaattcaga ggcgatttcg acgtcgcacg
      901 gaatacctgc cagcgattca aaccagtagc gggaaaccat accggagtta taagaagtac
      961 cacaggcgag gatctgaata tgctcaacct tcgacagcag ttcgtcggcg ttcggtccca
     1021 gctcgcttaa atcaacctga ccgtggctga tgcgcccagt aagggtgttt ttgatcgcgt
     1081 tcggctgttc gtagatctct ttctgcatgt agtgacggta aataccttta tcgcccgcgt
     1141 catattgcag attggattcg atatcctgac gttttacttc cgcgccagtt ttatcgaaga
     1201 tgtttaccga acggcgagta atttccgcaa tatcgccctc ttcaaggaag ataaagcgac
     1261 gggtcaccgg cagcagcgcc agctggtcag aagcgataaa gttttcgccc atacccaggc
     1321 caatcaccag cggactacca gaacgtgccg ccagcagggt atccgggtga cgggagtcca
     1381 tgatcactgt accgtacgca ccgcgcagct gcgggatagc acgcagaaca gcctcacgca
     1441 gagtcccgcc ttgttttagc tcccagttca ccagatgggc aatcacttcg gtgtcggttt
     1501 cagaaacgaa ggtatagcca cgcgctttta actcttcacg cagcggttca tggttttcga
     1561 tgatgccgtt atgcaccacc ataatgtgtt cagaaacatg cggatgcgcg ttagcttctg
     1621 aaggttcacc gtgggtcgcc cagcgagtgt gagcaatacc ggtgccgcca tgcagaggat
     1681 gttcttccgc tgcctgagcc agcatctgga ctttaccgag gcgacgcagg cgggtcatat
     1741 gaccttccgc atcaacaacg gccagaccag cagagtcata tccgcggtat tccagacgac
     1801 gtaaaccttc aagaaggatt tctgctacat cacgttgcgc gatcgcgcca acaattccac
     1861 acatagtttt tgattccgat ttatatcgtt gtcggtcaac ctgtatgccc gttttttcgg
     1921 gcgccccgag ccttgtagag agtggggtta tttttatagt tactgcttgt gggagggaga
     1981 ttatgttatc tcctcatccc agatgaccgg attacccggc cagaattact ttttctttac
     2041 cggacgacgc cagccttctt tctgagtctg cggcacacgg ctgatagcta atgcattttc
     2101 gccgacatta cgcgtcacag ttgtacccgc agcaatggtc gcgcctttgc ctactgttac
     2161 cggggccacc agctgagtgt cggaaccgac aaacacatcg tcgccgataa tggtcttaaa
     2221 tttattcgca ccatcgtagt tgcaggtaat ggttcccgcg ccgatgttaa cgttatcgcc
     2281 aatttccgca tcgcccaggt aagtcagatg accagctttc gagcctttac ccagacgcgc
     2341 ttttttcatc tcaacaaagt taccgacgtg tgcaccttcc agcaactcag caccaggacg
     2401 cagacgggca aacgggccaa tagtacaggc cgctgccaga ttcacgtctt ccacaacggt
     2461 atacggactg atttcgcaat catcgccaat cacgctgttt ttaatcacgc aaccggtgcc
     2521 gattttcacg cgatgaccga gagtcacgtt gccctcgatg ataacgttag tatcaatttc
     2581 aacatcgcgc ccgtgagtaa gcgtaccgcg cagatcaaaa cgcgccggat cgcgcagcat
     2641 aacgcctgct aacagcagtt tttcagcctg ttcggactgg taaacgcgct ccagacggga
     2701 gagttgcagg cggttattca caccttctac ttcgcttaaa cgttgcggat gaacggcgac
     2761 gatttcacgc ccttcctgat acgccagcgc aataatgtcg gtgatgtagt attcgccctg
     2821 agcattattg ttggtcagct tcgccagcca gcgtttcata tctgcgccgt tagagatcag
     2881 aatgccggtg ttgctctcct gaatctgacg ctgctcgtcg gtggcatctt tgtgctcaac
     2941 aatgccggta actttgccat tttcacgggt gatacgtcca taaccggtcg gatcatccag
     3001 tttcaccgtc agcagaccaa tgccaccctg cggtttagca tcacgcaggc gctggagtgt
     3061 ttcgacagag atcagcggca cgtcgccgta gagcattaaa atgtcttcat catcggcaaa
     3121 gaaaggtgcg gcctgctgca ttgcatgacc cgtacccagc tgctctgcct gaagcaccca
     3181 gttaaggttg tcgtctttca gcgcctgttt aagcagatcg ccgccgtgac cgtacaccag
     3241 gtgaacgtgc gctgcgccta attcattcgc agcatcaatg acatgctgaa ccatcgcttt
     3301 cccggcaagg gtatgcagca ctttcggaag atcggaatac atgcgcgtgc ctttgcctgc
     3361 ggcaaggatc actacgctca tagcattatt caacatacgc gtcctgactg taatttgaga
     3421 acgaatttaa accgcttcac cttgaaaaaa ctacattttt ttcatcgtga aatggacaga
     3481 ggataaattg ttcaataacg gattatcccc gctgacgaaa gtgccatttt cgaccatcgt
     3541 cacgcctttt gtctgcagaa aattaaggca gtaccttcat ttaagcgtca ggtggatgtt
     3601 tttgctctta tttcgatcaa tgaataaacg ggaaaacagg ggtgttttat atcagcagga
     3661 tctatgtgaa cgctcttcag gacggatcac gcgtaaaaaa aaaaccagcc tgtttccaga
     3721 ctggcttttg tgcttttcaa gccggtgtta catcgctttt ttggtcaact cgataacgcg
     3781 cagctgcgcg atcgctttgg ccagttccgc agacgcctga gcgtaatcta cgtcgccgtg
     3841 agagctgcta atgtgctctt cagccttacg tttcgcttcc atggctcgcg cttcgtcgag
     3901 atcctggccg cgaattgcgg tgtcggccag aacggtcacg ttgccagact gcacttcaag
     3961 aatgccgcca gacagataga taaactcttc gtgaccgtgc tgtttcacga tgcgaatcat
     4021 accaggctta atggcggtga gcagcggtgc gtggccaggg tagatcccca gttcaccttc
     4081 gctacccgtt acctggattt tctcgaccag accagagaac atttgttgct ctgcgctgac
     4141 gacgtccagg tggtaagtca ttgccatatc accctccgat taaggcgtta aagttttttg
     4201 gctttttcca cagcttcttc gatggaaccg accatgtaga acgcctgctc cggcaggtga
     4261 tcgtattcgc cttccatgat gcctttaaag ccacggatgg tgtctttcag ggagacgtat
     4321 ttacccggag aaccggtgaa tacttctgcc acgaagaacg gctgggacag gaagcgctgg
     4381 atcttacgag cacgcgctac caccagtttg tcttcttcag acagttcatc catacccagg
     4441 atggcgatga tgtctttcag ttcctgataa cgttgcagga tggactgaac gccacgcgcg
     4501 gtgtcgtagt gttcctgacc aaccaccagc gggtccagct gacggctggt ggagtccagc
     4561 gggtcaacgg ccgggtagat acccagagac gcgatctgac ggctcagtac cacggttgcg
     4621 tcaaggtgcg caaaggtggt tgccggagac gggtcagtca agtcatccgc aggtacgtat
     4681 accgcctgta cggaggtgat agaaccagtt ttggtggagg tgatacgttc ctgcagaacg
     4741 cccatctctt ccgccagggt cggctgataa cctaccgctg aaggcatacg gcccagcagt
     4801 gcggatactt ccgtaccggc cagggtgtaa cgatagatgt tgtcaacgaa cagcagaacg
     4861 tcacgacctt cgtcacggaa tttctcagcc atggtcagac cggtcagagc aacgcgcaga
     4921 cggtttcccg gcggctcgtt catctggcca tacaccaggg atactttgtc gataacgttg
     4981 gagtcggtca tttcgtggta gaagtcgtta ccctcacgag tacgttcacc tacgcccgca
     5041 aacacagagt aaccggagtg ctcgatcgcg atgttacgaa tgagctccat catgtttacg
     5101 gttttaccta cacccgcacc accgaacaga ccaactttac cgcccttagc gaacggacac
     5161 atcaggtcga taactttgat accggtttcc agcagttcct gagagtttga cagctcttcg
     5221 taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt caccgatctc gcctttcatg
     5281 tcgaccggtt cacccagtac gttcatgata cggcccagag tcgctttacc taccgggact
     5341 tcgatcgggt gttcgaggtc ttttacatcc agaccgcgac gcagaccgtc ggaggaaccc
     5401 attgcgatgg tacgcacgat accgccgccg agctgctgct gaacttccag caccagacgc
     5461 tcattaccat tttgcacctc aagagcatcg tacacgcgcg gtacggcatc ctgagggaat
     5521 tcgacgtcaa ctacggcgcc gattacctgg acaatctttc cagtagccat cttaaatcct
     5581 ctacgaaata acctgtttaa accgcggcgg cccccgagac gatctcggtg agttcctgag
     5641 taatgctggc ctgacgagct ttgttgtata ccaactgcag ctctttaatc aggctgccgc
     5701 cattgtcggt cgcggctttc atcgccacca tacgggcggc ctgctcgctg gccaggtttt
     5761 caaccacgcc ctgataaacc tgagattcga cataacgacg cagcagggta tccagcaacg
     5821 ccttcggatc gggttcgtac aggtaatccc aggatttatg tttcagatca tcatcatctg
     5881 atgccggtaa cggcagcagc tggctgatgg tcggaacctg agacatggtg ttaataaatt
     5941 tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc gtaggcctgc aacatcactt
     6001 ttaccggacc gatcagttcg gacagggaag ggttatcccc catgccggtg acctgggcaa
     6061 caacattgcc gcccacggag ttgaagaacg acacgccttt cgagccgatc attgcgaggt
     6121 cgcattgaac gcctttgtcg gtccaggtct tcatttccgc cagcagtttt ttgaacaggt
     6181 taatgttcaa accaccgcac aaaccacggt cggtcgacac caccaggtag cccacgcgtt
     6241 taacgtcgcg gtcttccagg taaaggtgct tatattccag attaccatgt gcaaggtgac
     6301 caatcacttt gcgcatggtt tctgcataag gacggctggc cgccatgcga tcctgcgatt
     6361 tacgcatttt ggaagcggcg accatctcca tcgctttagt gatcttttgc gtgttctgga
     6421 cgcttgcgat cttactacgt atctcttttg cgccggccat gagcttctcc tcaatgcctt
     6481 gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat tgggttgctt tgaaggaatc
     6541 gaggatgcct ttcagtttgc cttcgatttc gtcgttgtag ccaccggtct ggttgatctc
     6601 ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc agagcggctt cgaagctgcc
     6661 aattttcgac agttcaacat ccgccaggta accacgttct gctgcgaaca gaaccagaga
     6721 ctgctgcgca acggacatcg gcgcatactg tttctgtttc agcagttcgg tcactttctg
     6781 accgtggtca agctgcttac gtgttgcatc gtcaaggtcg gatgcaaact gagagaacgc
     6841 tgccagttca cgatactgtg ccagagcggt acggatacca ccggacagtt ttttcatgat
     6901 cttggtctgt gctgcaccac caacacggga tacggaaata cccgggttaa ccgcaggacg
     6961 aataccggcg ttgaacaggt tggtttccag gaagatctga ccatcggtaa tggagattac
     7021 gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt tcgataatcg gcagtgcggt
     7081 cagagaaccg gttttccctt tcacttcacc tttggtgaag gcttcaacgt attcggcgtt
     7141 aacacgtgca gcacgctcca gcagacgaga gtggaggtag aaaacgtcgc ccgggaatgc
     7201 ttcacgtcct ggcggacgac ggagcagcag ggagatctga cggtaagcaa cagcctgttt
     7261 agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg tcacggaagt attcgcccat
     7321 tgcgcaaccg gcatacggtg ccaggtattg cagtgcagcg gattcagacg cggttgctac
     7381 cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt ttacgtacca cgttagaaat
     7441 ggtggacgct ttctggccga tagcgacata gatacatttg ataccggaat cgcgctggtt
     7501 gatgatggca tcgatagcca gtgcggtttt acctgtctga cggtcaccga tgatcaattc
     7561 acgctgacca cgaccgattg ggatcatgga gtcaacggct ttataaccgg tctgtaccgg
     7621 ctgatctacg gactgacgtt cgataacgcc cggagcgatt gcttctacag cagagaagcc
     7681 gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc agagtgttaa ccacacggcc
     7741 cagcaggcca cggccaaccg gaacttccag gatacggcca gtacacttaa ctttcatgcc
     7801 ttcggcaagg tcagcgtacg gacccataac aaccgcacct acagagtcgc gctcgaggtt
     7861 cagtgcgata gcgtaacggt tacccggcag ggagatcatt tcaccctgca tacaatcggc
     7921 caggccgtga atgcggataa caccgtcact tacagaaaca atagtacctt cgttgtgagc
     7981 ttcactcaca acattgaact gagcaatgcg ctgcttgatc agttcgctga tttcggtgga
     8041 attcagttgc atgctccagt ccccttaaga ctgcaagacg tctgcaaggc gctcaagacg
     8101 accgcgtacg ctgccatcaa tgaccatatc acccgctcgg atgataacgc ctgccattac
     8161 agacttatcg attttgcaat tcagcttaac tttgcgtgac agacgttttt ccatcgcagc
     8221 agaaattttc gcgagctgtt gttcactcag tgcggcagcg gaaatgacgt ctacctcagc
     8281 ggtagcctca ctcacggcac gaaggtgaat aaactgctcc agaacatccg ggagcgcgtt
     8341 aagacgacca ttttcagcca ttacccgaat caggttctga ccgttttcgt ccagttgctc
     8401 accacaaact gcgataaacg actcggcgag cgtttctggc gcaagcgcgc cagagagaag
     8461 ctctgccatt tgttcgtttt tggttacctc ggcggcaaac gccagcatgt cctgccagcg
     8521 ttctacactt tggtgttcga cggcaaagtc aaaagctgct ttggcgtagg ggcgagctac
     8581 cgtaataaat tcagacatca gcccctccct ccttacagtt cagcgacaag tttatccacg
     8641 atgtcgctgt tagcagcttc atccacggaa cgttcgatga tcttctcggc gccagcaaca
     8701 gccaggatag caacttgctt acgcagctct tcacgggcac gtttacgctc ggcttcaatt
     8761 tctgcctgcg cctgggccac gattttagta cgttcctgtt ctgcctcagc tttcgcttcg
     8821 tccagaatct gcgagcggcg tttgttcgcc tgctcgatga ttacctgggc ttccgctttc
     8881 gcttttttca gctggtcggt cgcgctggcc tttgcaaggt caaggtcctt atgtgctcgt
     8941 tctgcggaag caaggccgtc agcaatttct ttttgacgtt tttcgatggc tgccattaat
     9001 ggcggccata cgtacttcat gcagaacaga acgaacagga caaacgcgat ggcctggccg
     9061 aggattgttg cgttaagatt cacagcacaa tgcctctatt tagttaacgt tctgatattg
     9121 ctctttaaat aaaagcaacg cttactacgc gacagcgaac atcacgtaca gacccagacc
     9181 tacagcgatc atcgggatag catccaccag acccataacg ataaagaact gagtacgcag
     9241 cagaggaatc agatcaggtt gacgcgctgc gccttccaag aatttacccc cgaggatgcc
     9301 gataccgatc gcagcaccga ttgccgccag acccatcatc acagcggcag ccatgtacag
     9361 cagatccata ttcaggtttt ccatgacagt ctccagtttg tttcagttaa aacgtagtag
     9421 tgttggtaaa ttaatgttct tcagacgcca tcgacagata gacgatcgtc agaaccatga
     9481 agatgaaggc ttgcagcgta atgatcagga tgtggaaaat ggcccacggc acattcagga
     9541 tccactgtga ccaccacggc aacagaccag caatcagaat gaaaatcagc tcaccggcat
     9601 acatgttacc gaacagtcgc aaaccgagtg aaactggttt ggacagcagg cttacccctt
     9661 caaggattaa gttgacagga atgaacgccc agtgattgaa cggctgcagc gtcaactctt
     9721 tcgtgaagcc gccgatgcct ttcattttga tgctgtagaa cagaatcagg ataaatacgc
     9781 ccaatgccat agacagcgtt acgttcacgt ccgcagacgg aaccacacgc agtgcaggca
     9841 gacccagtac atgttcagca atgtacggca gcaggtcgat aggcagtaaa tccatcaggt
     9901 tcatcaggaa tacccagacg aagatcgtca gggccagcgg agcaatcagc ttgcttttgc
     9961 catggtacat gtctttcacg ctaccattaa caaagccgat caccagctca atcgcggtct
    10021 gaaacttacc tggcacaccg ctggtcgcct ttttggctac gctacggaat aaaaccagga
    10081 acaacagacc cagcaccacc gagaagaaca tggagtcaat attgattgtc cagaaggtgg
    10141 ctggggggtt ttgtggatcc accagcgaga atgtacgcag gtccagctga aggttattca
    10201 ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga agccatgatg ccttttaccc
    10261 tttgttgtta attacagccg gtgccagtat ctgaaccacc agcaccaaaa cccacgtaac
    10321 gatcagcggc aagaataccg cctttaaaac cgccaacgcc accaccagta acaccaacat
    10381 cgccagaact ttgaaagctt cgccaaatgc gaatgtccag gccacccggc ctttcgctgg
    10441 tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa acgttaggca gaaagactgc
    10501 caggcccccg cttattgcag agacgcccca gaaggggtct ttgaggctga acagcaatcc
    10561 acttgctatc accaccagta actgaacgag cagaagcttc cgagcaacgt ttcgactcac
    10621 gagcgacaca gacatcacgt tttttactcc tgctcccttc gaggtatgcc gcgtgtcgta
    10681 taaaactttc tttaaggctt agagtcaagc atcaaaaagc ggtcaaatta tacggcgcgc
    10741 ccccgtgatt tcaaacaata agtagccaaa aggtgaataa atgtttaaat atttttccag
    10801 tgcatacaat tgcgactttt ctactaaccc tgttcgatca tgaaaaactg taaataacgc
    10861 gtaaacactg gtgataaagc gtgcttcaga tcacatattg cgcatgttcg cgcacagcat
    10921 atttatttac ttggcaaatg atgcctttgc aagtttatga tatttcagtc taaaaacaga
    10981 tactgtttta ataaatgaca tttacacaac aaaaaccacc cattgacatt tttaataatg
    11041 ttttagcagc caatgatggt tcttagcgcc gatttttagc agactgatat tttcactaat
    11101 gacttatttt ctgcttacca aaaaaagcca cgttatcttg ttgatgcaaa agagtgaacg
    11161 tggcgttaaa tgtaaccagt tatatcagta gaaaacctgg ttgttgttaa cagtctaacc
    11221 ggtcaatttt ttatgatttt tttgataaaa attaaatttt atttgcttta atcaccacca
    11281 gatgacgttc gccatccagg gctggaacct gaagtttaac cactgattcg acctgatatt
    11341 cttcgggcaa caaagcgatt tcatcttccg gcatttgccc tttcagcgcg tagaaacggc
    11401 cttgctcacc aggaagatgg tggcaccagc tcaccatatc gttcagagag gcaaaagcgc
    11461 ggctaattac gccatcaaat ggcggctctg aaggaaactc ttctaccctg ctctgtacag
    11521 gttcaatatt ctccagttta agctcatgtt gcacctgacg aaggaaacgc acgcgtttac
    11581 caaggctatc caacagagtg aaatgggctt caggacgcac gatagagagt ggaatgcctg
    11641 gcagtcctgg tccggtgccg acatcgataa accgttcacc ttgcagatac ggtgccacca
    11701 caatgctatc gagaatatgg cgtaccagca tctcattagg atcgcggacc gaagtcaggt
    11761 tgtacgcttt gttccattta tgcagcatat tcacgtaggc aataagctgg tttttctggt
    11821 gatcggtaag cgaaatacct gcgtctttca gcagtaagga gagtttgttg agcacggtga
    11881 ttacctgttc ttgatgcgtt gcctggtaag cgggtgctta ccaggcattt ttaatgcgtt
    11941 atgcgctacg acgcagcata ccctgttttt tcagccacac cagcagaatg gagatagccg
    12001 caggcgtgac gccagaaata cgcgaagctt ggccgataga ggctggtttg tgatcgttaa
    12061 gtttggcgat cacttcgtta gaaagaccgg atacctggcg gtaatccagt gtcgcgggta
    12121 gcagggtgtt ctcgttacgc agctgctttt cgatctcatc ttgctggcgc gcgatataac
    12181 cttcgtattt aacctgaatc tcaacctgtt ccgccgcctg ttcgtctgtc aacgcagggg
    12241 caaacggcgt cagcgtggtt aatttttcat aagtcatttc cggacgacgc agcagatctt
    12301 caccactggc ttcacgggaa agcggcgcag tcaggtgagc attcacttcg gctgcagctt
    12361 ccgccgacgg ggttacccag gtcgatttca gacgctgacg ctcacgctcg atattctcaa
    12421 gtttctcgtt aaagcgcgcc caacgttcgt catccaccag gcccagttca cgaccgattt
    12481 cagtcaaacg cagatccgca ttatcttcgc gtagcatcag acgatattct gcgcgcgaag
    12541 taaacatacg atacggttct ttggttccta aagtgcacag gtcatcaact agtacgccga
    12601 gatacgcctg agaacgtgcc ggagcccaac cttctttgtc agcagacaga cgggcagcgt
    12661 taagaccggc cagcaaacct tgcgcagcgg cttcttcgta accggtagtg ccgttaatct
    12721 gaccagcaaa gaacagcccc tggataaact tgctctccag cgtcggtttc aggtcgcgag
    12781 gatcgaagaa gtcatactca atggcataac ccggacgcac gatcttcgcg ttttccatcc
    12841 cctgcataga gcggacgatt tgcatctgca catcgaacgg caggctggtg gagataccgt
    12901 tcggataaat ttcattagag gtcagtcctt ccggttcaag gaagatctga tgctgatttc
    12961 tgtcggcgaa gcgcatgact ttgtcttcga tcgacgggca gtagcgtggg ccgacacctt
    13021 cgatcacccc tgcatacatt gggctacgat cgaggttact gcggatcaca tcatgggttt
    13081 tctcgttggt atgagtgata taacacggca cctgctgggg atgctgggac gcattgccca
    13141 taaacgagaa taccggcatt gggttatcgc catgctgttg cgccagcaca ctaaagtcga
    13201 tagttcgagc atcaatacgc ggtggtgtcc cggttttcag acgaccaacg cgcagcggca
    13261 gttcacgcaa acggcgagaa agcggaatgg acggcggatc accagcacgg ccaccgctgt
    13321 aattatccag accgatatga attttaccgt cgaggaacgt cccaacggtg agcacgactg
    13381 ctttggcacg gaacttcagt cccatttggg taacagcacc gaccacgcga tcgttttcga
    13441 caataagatc ttcaaccgcc tgctggaaga tcatcaggtt cggttggttc tccagcgccg
    13501 tacgtaccgc ctgacggtag agcacacgat ccgcctgagc tcgggtagcg cgaaccgccg
    13561 gtcctttgct tgcgtttagt atcctaaact ggatacccgc ttgatcgatc gctttcgcca
    13621 tcagaccgcc gagtgcatcc acttctttta ccagatgtcc cttcccaata ccgccgatcg
    13681 ccgggttgca gctcatctgc cccagagtgt cgatattgtg tgtcaaaagc agagtctgtt
    13741 gacccatacg cgccgcggcc atcgcggcct cggtgcctgc atgacccccg ccaatgatga
    13801 tgacgtcaaa aggatccgga taaaacatgg tgattgcctc gcataacgcg gtatgaaaat
    13861 ggattgaagc ccgggccgtg gattctactc aactttgtcg gcttgagaaa gacctgggat
    13921 cctgggtatt aaaaaggaga tctatttatt tagagatctg ttctattgtg atctcttatt
    13981 aggatcgcac tgccctgtgg ataacaagga tccggctttt aagatcaaca acctggaaag
    14041 gatcattaac tgtgaatgat cggtgatcct ggaccgtata agctgggatc agaatgaggg
    14101 gttatacaca actcaaaaac tgaacaaccg ttgttccttg gataactacc ggttgaacca
    14161 agcttcctga cagagttatc cacagtagaa cgcacgatct gtatacttat ttgagtaaat
    14221 taatccacga tcccagccat tcttctgccg gatcttccgg aatgtcgtga tcaagaatgt
    14281 tgatcttcag tgtttcgcct gtctgttttg caccggaatt tttgagttct gcctcgagtt
    14341 tatcgatagc cccacaaaag gtgtcatatt cacgactacc aataccgatt gcgccaaagc
    14401 ggaccgcaga aagatcgggc ttctgttcct gcaatgcttc atagaaagga gaaaggttgt
    14461 ccggaatatc tccggcaccg tgggtggagc tgataatcag ccagatcccc gaggcagata
    14521 aatcttccaa cagcggaccg tgcagcgttt cggtggtaaa acccgcctct tccagctttt
    14581 cagccaggtg ttctgctaca tattcggcac cgccgagggt gctgccgctg ataagagtga
    14641 tatctgccat aaaccgccac ctttattaag agtggcgtat tgtacgctgt gaacgcgttg
    14701 ggatctacct gtggaaaagt atgggattaa aaaagccgat cagggcttga tagtacgcat
    14761 gatcgggtgt tgcaggacga tcaatgtctc ggtggactga atttcatcaa ttgtttggat
    14821 cttgttgata agtacatgct ggagagcgtc gatcgaacgg cacattactt ttataaagat
    14881 gctgtagtgg cctgttgtgt aataggcttc agtgacttca tcaaggcttt ccagctttgc
    14941 cagcgcggaa gggtagtctt tggcgctctt taatataatg ccgataaagc agcctacgtc
    15001 ataaccgagc tgcttcgggc tgacatcaat acgcgccccg gtaatgatcc ccgcctgctt
    15061 cattttctct acccgaacgt gaatcgtccc cggactgacg ccaaattgtt tcgccagttc
    15121 ggcgtaagcg gtgcgcgcat tgcccattaa tgcttccagg atgccacggt ccagattgtc
    15181 gatcagataa ttttccatag gattttctta tgcagattga tgattcattc tattttagcc
    15241 ttctttttta atgaatcaaa agtgtgttag gctttttatt gaatgattat tgcatgtgtg
    15301 tcggtttttg ttgcttaatc ataagcaaca ggacgcagga gtataaaaaa tgaaaaccgc
    15361 ttacattgcc aaacaacgcc aaattagctt cgtgaaatct cacttttctc gtcaactgga
    15421 agaacgtctg gggctgatcg aagtccaggc accgattctt agccgtgtgg gggatggcac
    15481 gcaggataac ttgtcgggct gtgaaaaagc ggtgcaggta aaagtgaaag ctctgcctga
    15541 tgcccagttc gaagtggttc attcactggc gaagtggaaa cgtcagacct tagggcaaca
    15601 cgacttcagc gcgggcgaag ggctgtacac gcacatgaaa gcccttcgcc ccgatgaaga
    15661 ccgtctttct ccgttgcact cggtctatgt agaccagtgg gactgggaac gcgtaatggg
    15721 cgacggtgag cgtcaattct cgactctgaa aagcacggta gaggcgatct gggcgggaat
    15781 taaagcaacc gaagctgcgg ttagcgaaga gtttggcctg gcaccgttcc tgccggatca
    15841 gatccacttc gtacacagcc aggagttact gtctcgttat ctggatctcg atgccaaagg
    15901 gcgtgagcgg gcgatagcga aagatcttgg cgcggtattc cttgtcggga ttggcggcaa
    15961 gctgagcgat ggtcatcgcc acgacgtgcg cgcaccggat tatgatgact ggagcacccc
    16021 gtcagagctg ggtcatgcgg gtctgaacgg cgatattctg gtgtggaacc ctgtactgga
    16081 agatgcgttt gagctttctt ccatggggat ccgcgtggat gccgacacgc tgaagcatca
    16141 gctggcgctg accggtgatg aagatcgtct gcagctggaa tggcatcagg cgctgctgcg
    16201 cggtgaaatg ccgcagacca tcggcggcgg tatcggccag tctcgtttga ctatgctgct
    16261 gctgcaactg ccgcatatcg gccaggttca gtgtggagta tggccagctg ctgttcgcga
    16321 gagcgtccct tctctgctgt aataatttat cgccgccagc gtctgagcag gcggcttcgc
    16381 atcccggtat caaagcgcca gatatgatcg aaaatgcgca tgatgccggg tttgccgtgt
    16441 gccgacatcg ccacggcatg aaagcgatgc tgatgtaccc gctgcagctc tttcacttta
    16501 ctcgtcacgt cgtcaggcaa ccgctgagcg ataaaatcag aaatcaccac cgcatcggca
    16561 tcaaaccatt ccctgctttg caagcgttcc ataatggcgc gaaaacaact ggcaagatcg
    16621 gtgccgccac gaaactgctg gcttaaaaaa cggattgctt gttcgatgcc ttgtgggcct
    16681 gaaagctcat aacggacgat ctcggtggaa aatagcataa tatagcagcg ccggttttct
    16741 gcgagagcaa tgcgcatcaa ggccaggcag aacgctttcg cacactgttc attaaagccg
    16801 cccattgagc cggaagtatc cacacagaca ataaacggcc cgcgcggctg ttcatcgtaa
    16861 tctttatgta ccaccggacg ttcgatcact ttttcacgcc acgactcacc gtgcaggcga
    16921 taggtgagca actgtttttc caccagccga cggtaaaact catactccag ttccgttatc
    16981 cctagtgtcg ccagttctgg cggcaggaga cgtaaaatat catcgctttg ttgcagacca
    17041 tcaacctgct caggaaccgt cgccggttcg cgcaccatgg tgcggaaggt ttccatctgc
    17101 gcatcgttgc gcggtattga tttggcttcc cgagaacgcc ccagctgctc tgccaggcgt
    17161 ttcagttccg gctgttcgtt aagaaattca ccgtatttca caatcaactg atagtcgcca
    17221 cgtttaagct ggccggcgct catatcccac agacgaccag ctgcggtatt gttatctgcg
    17281 agaatcggtt caagttgtcc gctcagcgtc atgcgttcct gaacttcact taacagttgt
    17341 tcgcgttctt cttctaatag ctgttgattt aacgtcgttg cttgcacgat cagacttaaa
    17401 cgccagcgct ggagaaaaag cgtgtgtaac gctgaagtta ttgtgctgtt agcatcaacc
    17461 aactgccggg cttgttctgc ccatggggaa ttcagacgat gcagtaagtc caggatctgt
    17521 ggtagctgca caataaactg cggcgtggag aggagctggc tttgctgata gcacatcacc
    17581 tcttcggtga gttctggcgg gactcgggca tctttcagcc gactgcgcag cgcctcacgc
    17641 cagcggggaa catcatcagt gattgctgcc ttcagtcgtg ggaatttttc aaagaagacc
    17701 gccagctgcg gtgaggccag cagtgcgatg atcatctctt cgatcaatcc ctcttcgctg
    17761 acggccagca tcacattaag cgtatccagc gttagcattg ttgtgcctgg cgaatctgtg
    17821 caccgacatc ctgtaggctg gcttcaatgc gacctaacca gtcgccaggg ataaacagac
    17881 atttttgctg ttcgctgaat aaagcgtgtt gcttacgcca gtcgctttca agctcttcca
    17941 gttgttgttt tatttcacct ggcagacctt cagccgatga accgggaagt gccagcgtac
    18001 tgccttgtaa acttacatcg cgtacaacaa gatgttgggt gctatcaact tccagattca
    18061 gtttctgggc aaagccgata ccgttcagtt tgccgcgaat ttcaccacct ttactcagcc
    18121 actgttccag cgcgctacgc tcaaaggaga tatgaaccac ttccatatca tgcagtttta
    18181 acggtttttg cagcagcaga gtcagagtgg aagcagtaac gttaacaggg agttgatact
    18241 gctgacgacg gctaaaaatg ccgcccagac gaattaccgt taaggctgtt ttatcgcttt
    18301 gttgctgctg aagttgcagg tgacgttgca caatcgcgcc taggcgggtc aacatccctt
    18361 gctgttgcca ggcgtgaccg gtcatcaata catcaatttg ttgttgtatc aaattcaggc
    18421 tttgcgcgtc ataccacagg caatctttca gcaaaatgag atctaccggg gcaacagcac
    18481 tgcgaccgct aaaaaaggcg ctggcctgca ataatcggat cgcttttttc cagcgacgat
    18541 ccgagacata aggcgcatcc ggtaatttat ccagttgctg gcgcagcata aaaatcagct
    18601 caaatacatg atcgggcagc gtaatttcac caatctcttt ctgccagcgt tcatattctt
    18661 catctgtgac ctgcaaggca tcaggaaccg gattgtcgtt ttcatcctgt tgactggtca
    18721 gcatggagcg gaaattcgct ttatcctgca ctttatctaa ccacagacga atcagcatgc
    18781 ggtcatataa cgcttccaga ctgctgtctg cttccggcag ctcgttggag gccgccacca
    18841 gcagacgcat cgggattttt tctacgtgtg caccgttgcg gaactggcgc tcgttaatgg
    18901 cggtgagcaa ggtattaaga attgccgggc ccgctttcca gatctcatcc agaaagacaa
    18961 tttctgcttc cggcaggtaa ccgctggtta aacgttcata gcgcccttca tcttttagcg
    19021 cctgaataga aaggggacca aaaacttctt ccggcgtgga gaagcgggtc atcagatatt
    19081 caagcgcgcg ggcattctga aaggcgaatt ttaagcgccg ggcgatcaaa cttttggcaa
    19141 tacctggcgg gccaaggagg aacacacttt caccacttaa tgccgctaat aaacacaagc
    19201 ggatggcgtg gctacgttca taaagcccct tttccaacga actgctcagg cgggaaattc
    19261 tttccgctaa taaatgaggg tgagccataa tgaagtggcg tcctttcgtc aaaagttctg
    19321 cgtaaattgc gagtatagac gtttcttgct ggtggctaaa atagtctcaa agggg
//
LOCUS       DAJTYD010000064        19334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTYD010000064 DAJTYD010000000
VERSION     DAJTYD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.064"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..663
                     /gene="eutC"
                     /locus_tag="OO585_002029"
     CDS             <1..663
                     /gene="eutC"
                     /locus_tag="OO585_002029"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6629625.1"
                     /translation="ARVCTGRAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQG
                     LLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVN
                     YEELLPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQICEILGAKVVILLVGERPGLGQS
                     ESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINM
                     TR"
     gene            673..1332
                     /gene="eutL"
                     /locus_tag="OO585_002030"
     CDS             673..1332
                     /gene="eutL"
                     /locus_tag="OO585_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6629626.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1345..1845
                     /gene="eutK"
                     /locus_tag="OO585_002031"
     CDS             1345..1845
                     /gene="eutK"
                     /locus_tag="OO585_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6629627.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            1891..2943
                     /gene="eutR"
                     /locus_tag="OO585_002032"
     CDS             1891..2943
                     /gene="eutR"
                     /locus_tag="OO585_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6629628.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(2949..3848)
                     /gene="hemF"
                     /locus_tag="OO585_002033"
     CDS             complement(2949..3848)
                     /gene="hemF"
                     /locus_tag="OO585_002033"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6629629.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCNDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(3852..4721)
                     /gene="amiA"
                     /locus_tag="OO585_002034"
     CDS             complement(3852..4721)
                     /gene="amiA"
                     /locus_tag="OO585_002034"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6629630.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            4935..5360
                     /locus_tag="OO585_002035"
     CDS             4935..5360
                     /locus_tag="OO585_002035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6629631.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5347..5796
                     /locus_tag="OO585_002036"
     CDS             5347..5796
                     /locus_tag="OO585_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6629632.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6003..6578
                     /locus_tag="OO585_002037"
     CDS             6003..6578
                     /locus_tag="OO585_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6629633.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6674..7573
                     /gene="yfeX"
                     /locus_tag="OO585_002038"
     CDS             6674..7573
                     /gene="yfeX"
                     /locus_tag="OO585_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6629634.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7631..8935)
                     /gene="pbp4b"
                     /locus_tag="OO585_002039"
                     /pseudo
     CDS             complement(7631..8935)
                     /gene="pbp4b"
                     /locus_tag="OO585_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(8940..10364)
                     /gene="murP"
                     /locus_tag="OO585_002040"
     CDS             complement(8940..10364)
                     /gene="murP"
                     /locus_tag="OO585_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6629635.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10368..11264)
                     /gene="murQ"
                     /locus_tag="OO585_002041"
     CDS             complement(10368..11264)
                     /gene="murQ"
                     /locus_tag="OO585_002041"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6629636.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11428..12285
                     /gene="murR"
                     /locus_tag="OO585_002042"
     CDS             11428..12285
                     /gene="murR"
                     /locus_tag="OO585_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6629637.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12414..13205
                     /gene="ucpA"
                     /locus_tag="OO585_002043"
     CDS             12414..13205
                     /gene="ucpA"
                     /locus_tag="OO585_002043"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6629638.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13509..14525
                     /gene="cysP"
                     /locus_tag="OO585_002044"
     CDS             13509..14525
                     /gene="cysP"
                     /locus_tag="OO585_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6629639.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14525..15358
                     /gene="cysT"
                     /locus_tag="OO585_002045"
     CDS             14525..15358
                     /gene="cysT"
                     /locus_tag="OO585_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6629640.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15358..16233
                     /gene="cysW"
                     /locus_tag="OO585_002046"
     CDS             15358..16233
                     /gene="cysW"
                     /locus_tag="OO585_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6629641.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16223..17320
                     /gene="cysA"
                     /locus_tag="OO585_002047"
     CDS             16223..17320
                     /gene="cysA"
                     /locus_tag="OO585_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6629642.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17454..18365
                     /gene="cysM"
                     /locus_tag="OO585_002048"
     CDS             17454..18365
                     /gene="cysM"
                     /locus_tag="OO585_002048"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6629643.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18368..18736)
                     /locus_tag="OO585_002049"
     CDS             complement(18368..18736)
                     /locus_tag="OO585_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6629644.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            18841..>19334
                     /locus_tag="OO585_002050"
     CDS             18841..>19334
                     /locus_tag="OO585_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6629645.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELE"
BASE COUNT         4437 a         4911 c         5446 g         4540 t
ORIGIN      
        1 gcccgcgttt gtaccggtcg tgccggtccg cgtccgcgta cccaggcgtt gctgcgtttc
       61 ctggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg
      121 aaagcgcagg gcttgctgga agtacgctcc gaaattagcg acaaaaatct ctacctgact
      181 cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt
      241 gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc
      301 gtgaactacg aagagctcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa
      361 gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatctgtgag
      421 atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt gggtcagtca
      481 gaaagcctct cctgttacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat
      541 cgcacctgca tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc
      601 attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga
      661 taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg
      721 attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc
      781 ctcgggctga tttctgctga ttccgatgac gtcacctaca ttgcggctga cgaagcgacc
      841 aagcaggcga tggtcgaagt ggtatatggc cgctcgctgt atgcgggagc agcacacgga
      901 ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg
      961 cgtgctggtc tggatgcgat ggttgcgcat attgaaaatg gcgctgcttt ccagtgggct
     1021 aatgacgcag aaaacaccgc attcctggca catgtagttt cgcgtaccgg ttcttatctc
     1081 tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg
     1141 gaagcgacct acggcattga tgcagcgttg aaatctgccg acgtacagct ggtgacctat
     1201 gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg
     1261 tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc
     1321 cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc
     1381 atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc
     1441 agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg
     1501 ggtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc
     1561 cgcaaggaga tcggtcggcc agacgatgac acccagtggc tggttactgg ctttaaccgc
     1621 cagccgaagc aacccgtaag ggaacccgac gcgccagtta tcgtcgcgga atctgctgac
     1681 gagttgttgg cgctgttaac atcagtacgt cagggaatga cggcaggaga agtggctgcc
     1741 cactttggct ggccgctgga aaaagccaga aatgcgctcg aacagctctt ttctgccggg
     1801 acgttacgta aacgcagtag tcgttatcgt ctcaagcccc attaacctgt cggaggtgct
     1861 gggtgtccta caacacccgg cattaacatc atgaaaaaga cccgtacagc caatttgcac
     1921 catctttatc atgaaccctt acccgaaaac ctgaagctca cgccgaaggt cgaagtggat
     1981 aatgttcatc aacgacagac aacggatgtc tatgaacatg ctttaacgat taccgcctgg
     2041 cagcagattt acgatcagct gcatccgggc aagtttcatg gtgaatttac ggaaattcta
     2101 ctcgatgata ttcaggtttt tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg
     2161 gtctggccga actcgttctg gtttggcatt ccggcgacgc gcggtgagca gggatttatc
     2221 ggttcgcaat gtctgggaag cgcggaaatc gccacccgcc ctggtggcac tgaatttgaa
     2281 ctgagcacgc cggatgatta cacgatcctg ggcgtggtgc tttctgaaga tgtcatcacc
     2341 cggcaggcta actttttgca taacccggat cgggtattac atatgctgcg tagccagtcg
     2401 gcgctggaag tgaaagagca gcataaagcc gcgctgtggg gctttgtcca acaggcgctg
     2461 gcgacgtttt gcgagaaccc ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg
     2521 gataatttgc taatggcgat gggggcgatg ctggaagacg cgcaaccaat ggtgacggcg
     2581 gaaagcatca gtcatcagag ttaccgtaga ttactttccc gcgcccgtga atatgtgctg
     2641 gaaaatatgt ctgagccggt gacggtgctg gacttgtgta atcaactgca tgtcagtcgc
     2701 cgcacgctac aaaacgcgtt tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa
     2761 cgcattcgcc tgaacgccgt acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg
     2821 gtaaaagacg ccgccatgca gtggggattc tggcatctgg ggcaatttgt cacggattac
     2881 cagcagctgt ttgccgagaa gccgtcgttg acgttgcatc agcggatgcg ggaatggggg
     2941 tgagggagtt acacccaatc cctgacctta ataaactcac ttaacgccgc ttctgggctg
     3001 ccatcttttg gctgataatc atactcccag cgtaccagtg gcggcattga catcaggatc
     3061 gactcggtgc gcccgccagt ttgcaggcca aacagagtgc cgcgatccca gaccagattg
     3121 aactcgacat aacgaccgcg acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc
     3181 attgctttac gtcgctctac aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc
     3241 tgcataaagg caaaacagtg gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg
     3301 ccaataccgc gctgttcgtt gcgatgtttg aggtagaagt aatcgttgca ccactttttg
     3361 taacggggat aaacgtcttc accaaatggc aggcacaggt cacgggcggt gcggtgccag
     3421 tgaatggcgt cttcttcaaa accatagaaa ggggttaaat cgaagccgcc gccaaaccac
     3481 cagacgggct ctgcacccgg tttttcggca ataaaaaaac gcacattcgc gtggctggtg
     3541 ggaacatacg ggttatgcgg atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc
     3601 ccggcaagtt ccgggcgatg agcggtggcg gaagcaggca tcgcctcgcc gtggacgtga
     3661 gaaaagttga cgcctgcctg ttcgaaaaca ccaccattac gcaacacccg actacgcccg
     3721 ccgccgccag cttcgcgctg ccaactatct tcgacaaatt ctgcgccatc gacggcggtc
     3781 agctgctgac aaatcgtatc ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg
     3841 ggtttcataa cttatcgctt tttcgaatgt gctttctggt tgtcgaacca gtggaaataa
     3901 ctgatcacgc cttcagcaat cgctgtggcg attttctgac gaaacgccgc cgtgcctaac
     3961 agccgctctt cttccgggtt ggtgataaac gaggtttcca ccagcaccga aggaaccgac
     4021 ggcgatttca acaccacaaa cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc
     4081 ggcttaatct ttttcagaat atgcgagcca agcgtcaggc tgtttttaat agtatcggtt
     4141 tgcaccagat caaacagcac ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg
     4201 gcaacttcat cggcgcggtt ttcgcgttca gacaggtatt tcgccattgc gctacttgcc
     4261 ccacggttag agagggcaaa taccgaagca ccggcagctt tcgggttggt aaagccatcg
     4321 gcatgaattg acataaacag atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa
     4381 agtgggataa acgtatcgcc agaacgcgtt aaacgcgcat caatcccatg attacgcaaa
     4441 atggaacgga cgtttttagc aatcgccagc accacatgtt tttctttcga accgttgcgt
     4501 ccgatcgctc cggtatcaat tccgccgtgg cctggatcga gaacaacgac acgtttgccg
     4561 ccagattttt tggctttcgg cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg
     4621 gcgatggctt gcgacattcc tgacaacgtc agggcagcca aaccggcttt cagcacctgg
     4681 cggcgcgaag tgagtgtttt tagtggttta aaagtgctca tacggcctga gttgtaataa
     4741 taaagttcca gatgttatat cgtatcgcgt aatccgttac gacggtttga tgttgagaat
     4801 tgttttactt ttcatttcaa tagttgacaa ctccccatta tgccactttt tcaaccgatt
     4861 ttcgtcagat attggctaat tctaccgttc acgccataat cagcctttta acccgcaaaa
     4921 aggtaacgaa taccatggag atacgcgtat ttcgccagga agatttcgaa gaggtcatca
     4981 ccctttggga gcgttgcgac ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc
     5041 gtaagatgaa ccatgacgtc agtttgtttt tggtcgctga ggtgaacggt gaagtggtcg
     5101 gaacggtgat gggcggttat gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc
     5161 cagagtttcg tgggcgtggg attgccaatg cgttgcttaa tcggctggag aaaaagctga
     5221 ttgctcgtgg ctgcccgaaa attcagatca atgtgccgga agataacgac atggtgctcg
     5281 gaatgtatga acgcctggga tatgaacacg ccgacatgct gagtctgggt aagcgtttga
     5341 ttgaagatga agagtactga gttccatcct gtccattatg atgcgcacgg acgcctgcgt
     5401 ttacccttgc tcttctggct tgtgctattg cttcaggcgc gaacctgggt gctgtttatc
     5461 attgccggtg cgtcgcgcga gcagggtact gcgctgctaa atctgtttta tcccgatcac
     5521 gataatttct ggctggggtt gattcccggt attccggcgg tgctggcgtt tttgttgagc
     5581 ggtcggcggg cttcgtttcc tcgcacctgg catgtgctct attttctgtt gctgttggcg
     5641 caggtggttt tactttgctg gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt
     5701 atcggtctgg cgctggtagt ggcggatatt gtggcgttaa tctggctgct gaccaatcga
     5761 cgtttacgcg cttgttttaa tgaagaaaaa gaataacgca gaggtgcgct ttgtttatgc
     5821 cggatgcggc gtgaacgcct tatccggcct acaaaacatc gaaaattcaa tatattgcag
     5881 gagaaagtag gcctgataag cgtagcgcat caggcaattt tgtgtttgtc atcaaccttt
     5941 aacggcactt tttggtgaat ttgcactcca agcaacgtta ttgaataacc aaaggaagtg
     6001 acatgaaatc gctgcgttta atgttatgcg ctatgccgtt gatgctgacc ggctgttcca
     6061 cgatgtcgtc agttaactgg tcggctgcta acccgtggaa ctggtttgga tcgtccacca
     6121 aagtgagcga gcagggcgtg ggtgaattaa cggcgtccac accactgcaa gaacaagcca
     6181 ttgctgatgc gcttgatggc gattatcgcc tgcgcagcgg aatgaaaacc gcgaacggca
     6241 acgtggtgcg cttttttgaa gtgatgaaag gcgacaacgt ggcgatggtg attaacggcg
     6301 atcaaggcac gatcagtcgc attgacgtgc tggatagcga tattcctgct gacaccggtg
     6361 ttaaaatcgg tacaccgttt agcgaccttt acagcaaagc atttggcaat tgccaaaaag
     6421 ctgatggtga tgataatcgt gccgtcgaat gtaaagccga aggcagtcaa catattagct
     6481 accagttcag aggggaatgg cgtggtcctg aagggttaat gccttcggac gataccctga
     6541 aaaactggaa agtcagtaaa attatctggc ggcgttaatt tgcgtctgaa caaaccgccg
     6601 ccgcgaaaaa acacgtaaaa tagcgctcaa caatgccacg gattgcgtgg cattcttatt
     6661 ttcaggagga acaatgtctc aggttcagag tggcattttg ccagaacatt gccgcgcggc
     6721 gatttggatc gaagccaacg tgaaagggga agttgacgcc ctgcgtgcgg ccagtaaaac
     6781 atttgccgac aaactggcaa cttttgaagc gaaattcccg gacgcgcatc ttggtgcggt
     6841 ggttgccttt ggtaacaaca cctggcgcgc tctgagcggc ggcgttgggg cagaagagct
     6901 gaaagatttt ccgggctacg gtaaaggcct tgcgccgacg acccagttcg atgtgttgat
     6961 ccacattctt tctctgcgtc acgacgtaaa cttctctgtc gctcaggcgg cgatggaagc
     7021 ctttggtgac tgcattgaag tgaaagaaga gatccacggc ttccgttggg tggaagagcg
     7081 tgacctgagc ggctttgttg acggtacaga aaacccggcg ggtgaagaga cgcgccgcga
     7141 agtggcggtt atcaaagacg gcgtggatgc cggcggcagc tatgtgtttg tgcagcgttg
     7201 ggagcacaac ctgaagcagc tcaaccggat gagcgttcac gatcaggaga tgatgatcgg
     7261 gcgcaccaaa gaggccaacg aagagattga cggcgacgaa cgtccggaaa cctctcacct
     7321 cacccgcgtt gatctgaaag aagatggcaa agggctgaag attgttcgcc agagcctgcc
     7381 gtacggcacc gccagtggca ctcacggtct gtacttctgc gcctactgcg cgcgtctgca
     7441 taacattgag cagcaactgc tgagcatgtt tggcgatacc gatggtaagc gtgatgcgat
     7501 gttgcgcttc accaaaccgg taaccggcgg ctattacttc gcgccgtcgc tggacaagct
     7561 gatggcgctg taagcgtttc tgattctatg attaaaggcc agccagtaac aaaactgact
     7621 ggccttttta ttacttttgc ttcaacgccg catacacctg atcaactacc caaccataag
     7681 ttgcaatcgg taactgaccg ctttcgaaca tattgggatt tttttgtgga tcggcaactg
     7741 gcgaatgggg cttgttgctt aacatcacaa tcgccatatg attgaccgga tcgataacgg
     7801 ttactgtccc ggtccagcca gtgtgaccgt aggtttgcgg gcttgccagc gtgccaaacg
     7861 tcggcgtcat ggtggcatta ccattcacgc gccagccgag gccaaaagtg gcatcttcct
     7921 tagagctggt ggtgaacatc ttcaccgttt ccgcactgaa cagctgcaca tcgccatagc
     7981 ccccgccgtt cagcatcgtt tgcattaaca ccgcaatatc gccggtattg gaaaacaaac
     8041 ctgcgtgccc ggaaacgccg cccatcgaat agaaggcttt ttcatcgtgc acctgacccc
     8101 agagagtgga ggtgcggata ttcgggaaat ggatcacgcc atcgcgggta ttgccgttca
     8161 attccgtggc ggcaatttgt tgcggtttaa agcctttcag cagcgggtta aataccgtat
     8221 gtgtcaggcc gagcgggcga taaatcgact cttcaacata gcggtcaagc ggctgaccgg
     8281 taaccgactc gacgataaat ccaagcagca tataatcgac atcgctgtag atatgtttgc
     8341 tgccgggctg atattccagc ggcgtgcgct tgatcatctc cagcgtctgg cctttatcct
     8401 aggaatataa cgcgcccgcg accgctttat tcgggtattg cggatctgcc gggaaaccgc
     8461 cgctgtgatg cagcaggtca gaaatccgca gggtgttttt gcctttaatg gtgtcatttg
     8521 gactatcggc aaaccccgga atatatttcg caatccgatc gtcaggatgc agtttgcctt
     8581 cgctcatcag cttttgcagg gcgaagttcg tggcgtacat tttggtgttt gaggccaggt
     8641 catacagcgt cccggtggtg gctttgacag gctgttccat caacacgctg ccatcgtact
     8701 ttttcgccgc tccccaggct ttgcgataca caatctgatt atctttaatg atcagcaggt
     8761 ttacgccggg ataaccggca tcaacttgct ggctaatcca gcgatccatc tggttaagcc
     8821 gtttgacgtt aaaccctgct ttctctggcg agctttctgt cagaacaggg tattttgcgg
     8881 cgctgacgct acaactaaca gccagcagag aaagatagag cattgtccgt ttcatgatat
     8941 cagtccagat tgacgttacg gcagccaaag agcgtggtga aaataaaccc gcaaacccat
     9001 gccaccagaa tcccgccagc ataaaccgcc atggccggaa ggatgccttg tgcggaagtc
     9061 atcagcggca gcgctaccag accagacggc ccaaaggcgc tgtttaagcc catcggcaga
     9121 ccccaccagg ctatcaggcc gataaacaaa ccgcccgccg caccgcctaa acaggcggtg
     9181 acaaacggct tcatgcgggg cagggtgaca ccgtaaatca gcggttcacc aacgcccagc
     9241 aggccgggaa taatcgcccc gcgtacctga ctgcgtagcg cactgtgcgg ttgcgaccgc
     9301 cagtagagtg ccagcgccgc gcccacctgg cccgcgcctg ccattgaaag gatgggaaat
     9361 aagctgttga atccctggct gtccattaac gcgaggtaaa caggaataaa gccctgatgc
     9421 acgccaaata ccacggcgat caggaacagc ccggctaaaa ccgcacaacc gaacggatta
     9481 ctgttcaggt gcataaacag ccatgacata ccttcgaata accagccacc cagcggcata
     9541 atgatcaggt acgcaagcgt agcggtgatc agcagggtga ttaacgaggt cagcagcatg
     9601 tcgagatcgt ccggcataaa gcggcgcacc atgccttcaa tgcgggcgca ggcccacgcg
     9661 gcaatcagca cgccgataat attgccgcgc ggatcgatgg gcagaccaaa gaaatcgtga
     9721 aaaccggcgt agtaaccggt ggttgcagcg gggttataac cgagcaaaaa gagtgcggca
     9781 ataattgcgc cattgacgcc cgtgccgcca aatgcctggg cggcgttata gcccaccaga
     9841 atcaccagga aggtgaacaa acctttgctg aacaccttca taaaattcag cgcgtcgggt
     9901 agcgtgccct gagcatctgc cggaacgtgc atcaccgtgg caattaacgt cgctatcccc
     9961 agcagcagac cggcggcaat aaaaccgggg atcagcggcg tgaagatggt ggcgaatttg
    10021 gcgagaaatt gttgcacgcc ggaggtttgt ttggctttta actgacgttt gttctgggcg
    10081 gcgatttctg cggcatcctg taccggtgcc tcgcccagca gctcgctcat cgcttttgcg
    10141 gcacgatgcg ctttaccagg tccaaaaacg acctgcacct ggtcgctggt caaaatgacg
    10201 ctcttcacgc cttccagcgt tttgatattg ggatcaacca gtgaactgtc atgtacaccc
    10261 agacgcaggc gcgtcataca gttaccacaa ctggcgatat ttcccggtcc gccgacacgg
    10321 gtaagaatgg tgttcagaag ttcactgctg atctctttgg ccatgggtta ttccttgtct
    10381 aaaacctgac gaataaagcc gccgtgttga tccaggcgtt ttttagcttc tgcggcatcg
    10441 agatttttca gcaccatcac aatggccgtt ttacagttgc gctcgcaggc aattaacgcc
    10501 gcttccgctt gttcggcatt acatccggtg gcgtttttaa cgatattgac ctggcgcaca
    10561 tgcagttttt cgttggtggc gaccacatcg accataaggt tgccgaacac tttgccggat
    10621 ttaatcatca gcccggtgga aagcatattg agcaccagtt tctgcgctgt acctgctttc
    10681 atccgcgaag aaccggtaac aacttcggca cctacaatcg gtgtaatggc aaactcagcg
    10741 gtggttgaaa cggcgctccc cggattacag gaaattccca ctgtgcggca gccgagctgg
    10801 cgtgcgtatt ccagtccggc aatcacatac ggcgtgcgac cgctggcagc aatgccaacc
    10861 accacatcct gtgccgttaa attaatattt ttcagatcat taacaccgcc ttcccggcta
    10921 tcttccgcgc cttccaccgc gtgctgaatg gcatattcgc cgccagcaat caaaccaacc
    10981 accagacccg gtttcacgcc gtaggtgggc ggacattcgc tggcatccag aatccccaga
    11041 cgaccggatg ttcccgcacc gaggtaaatc agacgcccgc cgccgcttac ctgggcgtgg
    11101 ataacatcga tcgccgcggc gatatccggc agtacgcgct caacggcaag cggtacggtt
    11161 ttatcttcat cgttgataat ccggcacatt tccagcgttg atacgcggtc aatttcagcc
    11221 gaggcggcgt tcgagccttc agtaatcatc ttttcaagtt gcatcggtgg tgaccttact
    11281 atttacgatc aaataattgc agatgtatgg aatcatatat cctgaattga cagacttaaa
    11341 ggaatcattg agtctacact cgcagagtgt gaagagcgtc ataagaactt aagcggaatt
    11401 tatgctgcca gcccaggaga gtggaagatg ttgtacctga cgaaaataag caatgcgggg
    11461 agtgaattca ccgaaaacga gcaaaaaatc gctgactttc tgcaggccaa cgtgagtgag
    11521 ctgcaatccg tttcttcacg gcagatggcg aaacagctcg gcattagcca gtcgagcata
    11581 gtgaaatttg cccaaaaact cggcgcgcaa ggatttactg aattgcgtat ggcgttaata
    11641 ggcgaataca gtgccagccg ggagaaaacg aataccacgg cgctgcacct gcacagttcg
    11701 attaccagtg atgattcgct ggaagtgatt gcccgcaaat tgaatcgcga aaaagagctg
    11761 gcgctggagc aaacctgtgc gttgttcgac tacgcgcggc tgcaaaaaat cattgaggtc
    11821 atcagtaaag caccgtttat ccagataacg ggcctgggcg gatcggcgct ggtggggcgc
    11881 gatctgtcgt tcaaactgat gaaaattggc tatcgcgtgg cctgcgaagc cgacacgcac
    11941 gtacaagcca ccgtttccca ggcgttgaaa aaaggcgatg tacaaatcgc tatctcttac
    12001 agcggtagta aaaaagagat tgtcttgtgc gtagaagcgg cacgaaagca gggggcaacg
    12061 gtgattgcca ttacttctct ggctgactct ccgctgcggc ggctggcgca tttcacgctc
    12121 gataccgttt ccggtgaaac cgaatggcgt agctcgtcga tgtctacccg cacggcgcaa
    12181 aattctgtta ccgatttact gtttgttggt cttgtgcaac tcaatgatgt ggcgtcattg
    12241 aaaatgattc agcgcagcag tgagctaaca caacgcctga aatgatgcat aaagcagcga
    12301 ctggattgag attttcctga attagtgagc tgatccgcag caatattttg tttatcctgt
    12361 attttcagag ggaatggagt gtaacgctct gtattaacaa ggagagcatt aaaatgggta
    12421 aactcacggg caagacagca ctgattacgg gcgcattgca gggaattggc gaaggaattg
    12481 ccagaacttt tgcacgtcat ggcgcgaacc taatcttgct ggatatctcc cctgagatcg
    12541 aaaagctggc ggacgaactg tgtggtcgtg gtcatcgctg tacggcggtt gtcgccgatg
    12601 tgcgtgaccc ggcgtcggta gccgcagcta tcaaacgcgc gaaggaaaaa gaagggcgca
    12661 ttgatatcct ggtgaataac gcaggcgttt gtcgtctggg cagtttcctc gatatgagcg
    12721 atgacgatcg cgatttccat attgatatca atattaaagg cgtatggaac gtcacgaagg
    12781 cggtgctgcc ggagatgatt gcgcgcaaag atggtcgcat tgtgatgatg tcttcagtca
    12841 ctggtgatat ggtggccgat cctggcgaaa cggcgtacgc cttaacgaaa gcggcgattg
    12901 ttggcctgac aaaatcgctg gcggtggagt acgcgcagtc tggtattcgc gttaacgcca
    12961 tttgcccggg atacgtgcgc acaccaatgg cggaaagcat tgcccgccag tcgaacccgg
    13021 aagatccaga gtcggtgctg actgaaatgg cgaaagcaat cccgatgcgt cgcctcgccg
    13081 atccgctgga agtcggcgaa ctggcggcct tcctcgcatc ggatgaatcc agctatttaa
    13141 ccggtacaca gaatgtgatt gatggcggca gcacactgcc ggagacggtt agcgtcggta
    13201 tctgattcac ctctgtttcc tccctgcatg tgtggggagg atttcgtctt gaactaagtt
    13261 caccaggcta ttttatttgt cattttggcc ccgggcagtg ctcgaaatac tcacgtacta
    13321 tgtgtacgct ccggtttctc cgcgctgtcc gtgtccaaac tgcctgcaac aattacgcct
    13381 gttgaaccaa gttcttattc cctttttaac ttccaaatca ccaaacggta tataaaaccg
    13441 ttactccttt cacgtccgtt ataaatatga tggctattag aaagtcatta aatttataag
    13501 ggtgcgcaat ggccgttaac ttactgaaaa agaactcact cgcgctggtc gcttctctgc
    13561 tgctggcggg ccatgtacag gcaacggaac tgctgaacag ttcttatgac gtctcccgcg
    13621 agctgtttgc cgccctgaat ccgccgtttg aacagcaatg ggcaaaagat aacggcggcg
    13681 acaaactgac gataaaacaa tctcatgccg ggtcatcaaa acaggcgctg gcaattttgc
    13741 agggcttaaa aaccgacgtt gtcacttata accaggtgac cgacgtacaa attctgcacg
    13801 acaaaggcaa gctgatcccg gctgactggc agtcgcgcct gccgaataat agctcgccgt
    13861 tctactccac catgggcttc ctggtgcgta agggcaaccc gaagaatatc cacgactgga
    13921 acgacctggt gcgctccgac gtgaagctga ttttcccgaa cccgaaaacg tcgggtaacg
    13981 cgcgttatac ctatctggcg gcatggggcg cagcggataa agctgacggt ggcgacaaag
    14041 ccaaaaccga acagtttatg acccagttcc tgaaaaacgt tgaagtgttc gatactggcg
    14101 gtcgtggcgc gaccaccact tttgccgagc gcggcctggg cgatgtactg atcagcttcg
    14161 agtcggaagt gaacaacatc cgtaaacagt atgaagcgca aggctttgaa gtggtgattc
    14221 cgaaaaccaa cattctggcg gaattcccgg tggcgtgggt cgataaaaac gtgcaggcca
    14281 acggtacgga aaaagcagca aaagcctacc tgaactggct ctacagcccg caggcgcaga
    14341 ccatcatcac cgactattac taccgcgtaa ataacccgga agtcatggac aaactgaaag
    14401 ataaattccc gcagaccgag ctgttccgcg tggaagacaa atttggctcc tggccggaag
    14461 tgatgaaaac ccacttcacc agcggcggcg agttagacaa gctgttagcg gcggggcgta
    14521 actgatgttt gcagtttcat caagacgcgt gctgccgggc tttaccttaa gcctcggcac
    14581 cagtctgctg tttgtgtgcc tgattttgct gctgccgctc tccgcgctgg tgatgcagct
    14641 ggctcagatg agctgggcgc agtactggga ggtgatcacc aacccacaag tggtcgcggc
    14701 ctacaaagtg acgctgctgt cggcgtttgt ggcatcgatt tttaacggcg ttttcggtct
    14761 gctgatggcg tggattttaa cacgttatcg ctttccgggc cgcacgctgc ttgatgcgct
    14821 gatggattta ccctttgcgc tgccaacggc agtcgccggt ttaacgctgg cctcgctctt
    14881 ttccgtaaac ggtttttacg gtgaatggct ggcgaagttt gatatcaaag tcacctatac
    14941 ctggcttggt attgcggtgg cgatggcctt taccagcatt ccgtttgtgg tgcgtaccgt
    15001 gcagccggtg ctggaagagt taggcccaga atatgaagaa gcggcggaaa cgcttggtgc
    15061 aacgcgctgg cagagtttct gcaaagtggt gctgccggaa ctttctccag cgctggtggc
    15121 gggcgtggcg ttgtcgttta cccgtagtct tggtgagttt ggcgcggtga tatttatcgc
    15181 cgggaacatc gcttggaaga cggaagtgac gtcgctgatg atttttgtgc gcttgcagga
    15241 gtttgattac ccggcggcga gcgcgattgc ttcggtgatc ctcgcggcat ccctgctgct
    15301 gctgttctca attaacactc tgcaaagtcg ctttggtcgg cgtgtggtag gtcattaatg
    15361 gcggaagtta cccaattgaa gcgttatgac gcgcgcccga ttaactgggg caaatggttt
    15421 ctgattggca tcgggatgct ggtatcggcg ttcatcctgc tggtgccgat gatttatatc
    15481 ttcgtacagg cgttcagcaa agggctgatg ccggttttac agaatctggc cgatccggac
    15541 atgctgcacg ccatttggct gacggtgatg attgcgctga ttgccgtacc ggtgaacctg
    15601 gtgttcggca ttctgctggc gtggctggta acgcgcttta acttccccgg acgccagtta
    15661 ctgctgacgc tactggacat tccgtttgcc gtatcgccgg tggttgcagg tctggtgtat
    15721 ctgctgttct acggctctaa cggtccgctg ggcggctggc tggatgaaca taatttgcag
    15781 attatgttct cctggccggg aatggtgctg gtcaccatct ttgtgacctg tccttttgtg
    15841 gtgcgcgagc tggtgccggt gatgttaagc cagggcagcc aggaagacga agcggcgatt
    15901 ttgcttggcg cgtctggctg gcagatgttc cgtcgcgtca cattgccgaa cattcgctgg
    15961 gcgctgctgt atggtgtggt gctaaccaac gcccgcgcga ttggcgagtt tggtgcggtg
    16021 tcggtggttt ccggctcgat tcgcggcgaa acactttcgc tgccgttaca gattgaattg
    16081 ctggagcagg actacaacac cgtcggctcc tttaccgccg ccgcgctgtt gacgctgatg
    16141 gcgattatca ccctgttttt aaagagtatg ttgcagtggc gtctggagaa tcaggaaaaa
    16201 cgcgcgcagc aggaggagca tcatgagcat tgagattgcc aatattaaga agtcgtttgg
    16261 tcgcacccag gtgctgaacg atatctcact ggatattcct tctggtcaga tggtcgcgct
    16321 gctggggccg tccggttccg gaaaaaccac gctgctgcgc attattgccg ggctggagca
    16381 tcaaaccagc gggcatattc gcttccacgg taccgacgtg agccgcctgc acgcacgcga
    16441 tcgtaaagtc ggtttcgtgt tccagcatta cgcgctgttc cgtcatatga cggtgttcga
    16501 caatatcgct tttggcctga cggtgctgcc acgtcgcgag cgcccgaatg ccgcagccat
    16561 caaagcgaaa gtgacaaaat tgctggagat ggtccagctt gcgcatctgg cggatcgtta
    16621 tccggcacag ctttccggcg gccagaaaca gcgcgtggcg ctggcgcgcg cgctggctgt
    16681 tgaaccgcaa attctcctgc ttgatgaacc gtttggcgcg ctggatgcgc aggtgcgtaa
    16741 agagctgcgt cgctggctgc gtcaactgca tgaagagctg aaattcacca gcgtgttcgt
    16801 gacccacgac caggaagaag cgaccgaagt agctgatcgt gtagtggtga tgagccaggg
    16861 caatatcgag caggctgatg cgccggacca ggtatggcgc gaaccagcga cccgatttgt
    16921 gctcgaattt atgggcgaag tgaaccgcct gcagggaacc attcgcggcg ggcagttcca
    16981 tgttggcgca catcgctggc cgctggggta tacgcctgcg tatcaggggc cggtggatct
    17041 cttcctgcgc ccgtgggaag tggatatcag ccgccgtacc agtctcgatt cgccgctgcc
    17101 ggtacaggta ctggaagcca gcccgaaagg tcactacacc caattagtgg tgcagccgct
    17161 ggggtggtac aacgaaccgc tgacggtcgt gatgcatggc gacgatgccc cgcagcgtgg
    17221 cgagcgttta ttcgttggtc tgcaacatgc gcggctgtat aacggcgacg agcgtatcga
    17281 aacccgagat gaggaacttg ctctcgcaca aagcgcctga taggttgagt gaatgttaaa
    17341 cgcccggagg cgcttcccgc gatccgggct ttttaatggc aaggtttgta acctgtagac
    17401 ctgataagac gcgcaagcgt cgcatcaggc aacaccacgt atggatagag atcgtgagta
    17461 cattagaaca aacaataggc aatacgcctc tggtaaagtt gcagcgaatg gggccgaata
    17521 acggcagtga agtctggttg aaactggaag gcaataaccc ggcaggttcg gtgaaagatc
    17581 gtgcggcact ttcgatgatc gtcgaggcgg aaaagcgcgg ggaaattaaa ccgggtgatg
    17641 tgttaatcga agccaccagt ggtaacaccg gcattgcgct ggcaatgatt gccgcgctta
    17701 aaggctatcg catgaaattg ctgatgcccg acaacatgag ccaggaacgc cgtgcggcga
    17761 tgcgtgctta tggtgcggaa ctgattcttg tcaccaaaga gcagggcatg gaaggtgcgc
    17821 gcgatctggc gctggagatg gcgaatcgtg gcgaaggaaa gctgctcgat cagttcaata
    17881 atcccgataa cccttacgcc cattacacca ctaccgggcc ggaaatctgg cagcaaaccg
    17941 gcgggcgcat tacccatttt gtctccagca tgggaacaac cggcactatc accggcgttt
    18001 cacgctttat gcgcgaacaa tccaaaccgg tgaccattgt cggcctgcag ccggaagaag
    18061 gcagcagtat tcccggcatt cgccgctggc ctgcggaata tctgccgggg attttcaacg
    18121 cttctctggt ggatgaggtg ctggatattc atcagcgcga tgcggaaaac accatgcgcg
    18181 aactggcggt gcgggaagga atattctgtg gcgtcagctc aggcggcgcg gttgctggag
    18241 cactgcgggt ggcaaaagct aaccctggtg cggtggtggt agcgatcatc tgcgatcgtg
    18301 gcgatcgcta cctctccacc ggagtgtttg gggaagagca ttttagccag ggggcgggga
    18361 tttaagatta ttgcgcaggc accgttttat ccgtctgcgc aattagcgca tgtaaaaaag
    18421 gctgtgcgtt ctcatcgctc ttaccgggga tcttcacaat atacggcttc ccggtaatcg
    18481 atgacgacga agcaacctta tcaataaact gctcggcagt gtcaatgcgg ttacgggtat
    18541 tgccgagctt cagacgcaga tgagaaaccg cttcatcgca ggtatgttca tcaccgttgc
    18601 gcacaaatat caaatccttt ttctgtgata atccctccag catggcgttg atgcgggctt
    18661 cttcatgggc agttaacttc gcgtaagcgg ggagtgtcat tagtagtgta atgaccagac
    18721 aaatgatttt cttcattgaa ttaccttatc cttgttttgt aatagccgat ttgactgcca
    18781 gaatcaggta aagtttcggt tggaatacga tgaggatcat cggcaaggag aaaaaattaa
    18841 atgagtagtt tgttgttgtt taacgataag agcagggcgc tgcaggcgga tatcgtcgcc
    18901 gtgcagtcgc aggtggttta cggcagcgtg ggcaacagca ttgccgtgcc tgctatcaaa
    18961 cagaacggtc tgaatgtttt tgccgtgccg acggtattgc tgagcaatac gccgcattat
    19021 gacactttct acggtggtgc gattccggat gaatggttta gcggttattt gcgtgcgctt
    19081 caggagcgtg atgcgctgcg ccagctgcgc gctgtaacca cgggctatat gggaacggca
    19141 tcgcaaatca aaatccttgc cgagtggctg actgcgctac gcaaagacca tcctgaccta
    19201 ttgatcatgg tcgatccggt gattggcgat attgatagcg gaatttatgt caaacctgac
    19261 cttcccgagg cgtatcgaca atatttactg ccgctggcgc agggaattac ccccaatatc
    19321 tttgagctgg aaat
//
LOCUS       DAJTYD010000065        19318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTYD010000065 DAJTYD010000000
VERSION     DAJTYD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.065"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1058
                     /locus_tag="OO585_002051"
     CDS             <1..1058
                     /locus_tag="OO585_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6629646.1"
                     /translation="VAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLML
                     LAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIIC
                     QAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPL
                     GIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALA
                     LAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQA
                     VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTWH
                     DFLPSGTASVSEKHSER"
     gene            1048..2763
                     /gene="hyfG"
                     /locus_tag="OO585_002052"
     CDS             1048..2763
                     /gene="hyfG"
                     /locus_tag="OO585_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6629647.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            2773..3312
                     /gene="hyfH"
                     /locus_tag="OO585_002053"
     CDS             2773..3312
                     /gene="hyfH"
                     /locus_tag="OO585_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6629648.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            3309..4067
                     /gene="hyfI"
                     /locus_tag="OO585_002054"
     CDS             3309..4067
                     /gene="hyfI"
                     /locus_tag="OO585_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6629649.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            4060..4473
                     /gene="hyfJ"
                     /locus_tag="OO585_002055"
     CDS             4060..4473
                     /gene="hyfJ"
                     /locus_tag="OO585_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6629650.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            4503..6515
                     /gene="hyfR"
                     /locus_tag="OO585_002056"
                     /pseudo
     CDS             4503..6515
                     /gene="hyfR"
                     /locus_tag="OO585_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            6537..7382
                     /locus_tag="OO585_002057"
                     /pseudo
     CDS             6537..7382
                     /locus_tag="OO585_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(7420..8481)
                     /locus_tag="OO585_002058"
     CDS             complement(7420..8481)
                     /locus_tag="OO585_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6629651.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            8694..10157
                     /gene="bepA"
                     /locus_tag="OO585_002059"
     CDS             8694..10157
                     /gene="bepA"
                     /locus_tag="OO585_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6629652.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            10178..10537
                     /gene="arsC"
                     /locus_tag="OO585_002060"
     CDS             10178..10537
                     /gene="arsC"
                     /locus_tag="OO585_002060"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6629653.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLANSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(10675..11421)
                     /gene="hda"
                     /locus_tag="OO585_002061"
     CDS             complement(10675..11421)
                     /gene="hda"
                     /locus_tag="OO585_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6629654.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11471..12760)
                     /gene="uraA"
                     /locus_tag="OO585_002062"
     CDS             complement(11471..12760)
                     /gene="uraA"
                     /locus_tag="OO585_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6629655.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(12846..13472)
                     /gene="upp"
                     /locus_tag="OO585_002063"
     CDS             complement(12846..13472)
                     /gene="upp"
                     /locus_tag="OO585_002063"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6629656.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            13796..14833
                     /gene="purM"
                     /locus_tag="OO585_002064"
     CDS             13796..14833
                     /gene="purM"
                     /locus_tag="OO585_002064"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6629657.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            14833..15471
                     /gene="purN"
                     /locus_tag="OO585_002065"
     CDS             14833..15471
                     /gene="purN"
                     /locus_tag="OO585_002065"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6629658.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            15643..17709
                     /gene="ppk1"
                     /locus_tag="OO585_002066"
     CDS             15643..17709
                     /gene="ppk1"
                     /locus_tag="OO585_002066"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6629659.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            17714..19255
                     /gene="ppx"
                     /locus_tag="OO585_002067"
     CDS             17714..19255
                     /gene="ppx"
                     /locus_tag="OO585_002067"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6629660.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         4669 a         5055 c         5052 g         4542 t
ORIGIN      
        1 atgtcgcttt tggtctgttc ggtaccgtgc tggtatacgc caacgccgcc agcgttatgc
       61 cgcaggcaga aatggcgata ttctggagcg aggttcttaa gcaatcgtcc ttgcttgacc
      121 caacattaat gctgttggcc tttgtgtttg tgctaattgg ctttggtacc aaaaccgggc
      181 tatttcccat gcacgcctgg ctgccggatg ctcacagtga agcgccgagt ccggtcagcg
      241 ccctgctctc cgccgtattg ctgaactgcg cgctgttggt gctgattcgc tattacatca
      301 ttatttgcca agccatcggc agcgatttcc ccaaccggtt gttgctcatc ttcggcatgt
      361 tgtcggttgc cgtggcggca tttttcattc tggtacagcg ggacattaag cgtctgctgg
      421 cgtactccag cgtggagaac atggggctgg tcgcggtggc gctaggcatt ggcgggccgc
      481 tgggaatttt tgccgcgctg ctgcacacct taaaccacag tctggcaaaa acgctgctgt
      541 tctgcggttc cggcaatgta ctgctcaagt acggcacgcg cgatctcaac gtcgtctgtg
      601 ggatgctcaa aatcatgcca tttaccgccg tgctgtttgg cggcggtgcg ctggcgctgg
      661 cagggatgcc gcccttcaac atttttctta gcgaatttat gaccgttacc gcaggactgg
      721 cacgtaatca cctgctgatt atcgtcctgc tgttattgct gttaacgctg gtgctggcgg
      781 gcctagtacg gatggctgcg cgggtgttaa tggcgaaacc gccgcaggcc gttaaccggg
      841 gtgatctcgg ctggttgacc acctcgccaa tggtgattct gctggtcatg atgctggcga
      901 tgggaacgca tattccacaa cctgtcatca ggatcctggc gggcgcttcc actatagtcc
      961 tctcagggac gcacgacctg cctgcacaac gtagcacctg gcatgatttt ttgccttcag
     1021 gcaccgcatc tgtttcggag aaacacagtg aacgttaatt catcgtcaaa tagtggcgaa
     1081 gcgattctcg ccgccctgaa aacgcagttc cccggcgcgg tactggatga agagcgacaa
     1141 acgcctgaac aggtcaccat tacggtgaaa atcaatctgc tgcctgacgt tgtacattat
     1201 ctttattatc aacatgatgg ctggcttcca gtcctgtttg gcaacgacga gcggacactt
     1261 aacggtcatt acgcggttta ttatgccctt tcaatggaag gggccgaaaa atgctggatc
     1321 gtggtgaagg cactggtcga tgccgacagt cgggagtttc cgtcagtcac accgcgcgtc
     1381 cctgccgcgg tctggggcga gcgagaaatt cgcgatatgt acgggctgat tccggttggc
     1441 ctgccggatc agcgtcgcct ggtgttgccc gatgactggc cggaagatat gcatccgctg
     1501 cacaaagatg cgatggatta tcgactgcgc cctgaaccga cgacagattc cgaaacgtat
     1561 gcgtttatca atgagggcaa cagcgatgcg cgggtgatcc ctgtcggccc gctgcatatc
     1621 acctccgatg aaccgggtca cttccgcttg tttgtggatg gcgagcaaat tgtcgatgct
     1681 gattaccgcc tgttttatgt ccatcgaggc atggagaaac tggcagaaac gcggatgggc
     1741 tacaacgaag tgaccttctt atcggaccgc gtgtgtggga tttgcggttt tgcccacagt
     1801 gtggcctata ccaactcggt tgaaaatgca ctggggattg aggtgccgca acgagcgcat
     1861 accattcgct cgattctgct ggaagtcgaa cggctacaca gtcatttgct caaccttggc
     1921 ctctcctgcc attttgttgg ttttgatacc ggctttatgc aatttttccg cgtgcgggaa
     1981 aagtcgatga cgatggcgga attgctgacc gggtcgcgta aaacctacgg tctgaatctg
     2041 attggtggtg ttcgccgcga tattctcaaa gagcaacgtc tgcaaacgct gaaactggtg
     2101 cgcgagatgc gcgccgacgt gtcggagctg gtagagatgc tgcttgctac gccgaatatg
     2161 gaacaacgca ctcagggcat tggcattctc gaccgacaaa tcgcccgtga ttatagccct
     2221 gtagggccgc tgatccgcgg cagtggtttt gcccgtgatt tgcgctttga tcacccctac
     2281 gccgactacg gcaatattcc aaaaacactg tttaccttca ccggcggcga tgttttctcc
     2341 cgcgtgatgg tccgtgtcaa agagacgttt gattcgctgg caatgctgga atttgccctc
     2401 gacaacatgc cggatacccc actgctgacc gaaggcttta gctataaacc tcacgcattc
     2461 gcgctgggct ttgttgaagc gccacgcggt gaagacgtgc actggagcat gctcggtgat
     2521 aaccaaaaat tgttccgctg gcgctgccgt gccgccacct acgccaactg gccggtgttg
     2581 cgttacatgc tgcgcggcaa taccgtttct gacgcaccgc tgattatcgg tagccttgat
     2641 ccctgctact cctgtaccga ccgtgtgacg ctggtagatg tgcgcaagcg ccagtcaaaa
     2701 accgtgccgt ataaagagat cgaacgctac ggcattgatc gtaaccgttc gccgctgaag
     2761 taaggacaga agatgctgaa gttactgaaa actattatgc gcgccggaac cgcgacggtg
     2821 aaatatccct tcgcgccact ggaggtcagc ccaggctttc gcgggaaacc ggacctgatg
     2881 cccagccaat gtattgcctg cggcgcctgt gcttgtccgg caaatgcgct gactatccag
     2941 accgacgacc agcaaaattc gcgcacctgg cagctctatc tggggcgttg tatttactgc
     3001 ggacgttgtg aagaagtgtg cccgaccaga gccatccagc ttaccaataa ctttgaactg
     3061 accgtcacca ataaagccga tctctatacc cgcgcgacgt tccatctaca acgttgcagc
     3121 cgttgcgaac gcccgtttgc cccgcaaaaa accgtcgcac tggctgctga attgttggca
     3181 cagcaacaaa atgcgccaca aaaccgcgaa atgttgcggg cgcaagcgag cgtctgcccg
     3241 gagtgcaaac aacgcgcgac gctgatcaac gacgatacag atgtaccgct ggtggctaag
     3301 gagcagctat gagtccagtg cttacacaac atgtcagcca gcccatcacg ctggatgagc
     3361 aaacgcaaaa gatgaagcgg catttgctac aggatatccg tcgctcagcc tacgtttatc
     3421 gcgtcgattg cggcggctgc aacgcctgtg aaatcgaaat ttttgctgcc atcacccccg
     3481 tattcgacgc agaacgtttt ggcattaagg ttgtttcatc accgcgtcac gccgatattt
     3541 tgttatttac tggcgcagtc acccgggcga tgcgtatgcc tgcacttcgg gcgtatgagt
     3601 ctgcccccga tcataaaatc tgtgtttcct acggcgcgtg cggtgtcggc ggcggtattt
     3661 tccacgatct ctacagcgtc tggggcggca gcgacaccat tgtccccatt gatgtttgga
     3721 tccccggctg cccgccaaca ccggccgcca ccattcacgg tttcgccgtg gcgctcggtt
     3781 tgctgcaaca gaagattcac gctgtggatt atcgcgatcc caccggggtg actatgcaac
     3841 cgttgtggcc acagatcccg ccatcacagc gtatcgccat tgagcgagaa gcgcggcggc
     3901 tggcgggcta tcgtcagggg cgagaaattt gcgatcggct cctgcgccat ttaagcgacg
     3961 atcctaccgg aaatcgggtt aacacctggt tgcgcgatgc cgacgatcca cgtctcaata
     4021 gtatcgttca gcaactcttt cgcgtactcc gggggttaca tgactgaaga gtgcggggaa
     4081 attgttttct ggacgctgcg aaaaaagttt gtcgccagta gcgacgagat gccggaacac
     4141 cgctctcagg taatgtatta ctcgctggct atcggccatc acgttggcgt gattgattgt
     4201 ctgaatgtcg ccttccgctg cccactgacg gaaaacgaag attggcttgc acaggtcgaa
     4261 gaggagcaag cccgacgtaa gatgctgggg gtgatgacct ttggtgagat tgttattgac
     4321 gccagccaca ccgccctgtt gacccgggca ttcgcgctac tggcagatga cgcgacgtct
     4381 gtgtggcagg cgcgtagcat tcaattcatt catctgttgg atgaaattgt gcaggaaccg
     4441 gccatctatc tgatggccag aaaaattgcg tgagaaggat ttctcattaa taaggactgt
     4501 tgatggctat gtcagacgag gcgatgtttg ccccgccaca aggaataaca attgaagcgg
     4561 taaacggaat gctcgcggag cggttagcac agaaacacgg taaggcgtct ttattacgcg
     4621 ccttcatccc gatgccgccg ccgttcagcc cggtacaact tattgaactg catgttatca
     4681 aaagcaactt ctattaccgc taccatgatg atggcagcga tgtgacggca acaacagagt
     4741 atcagggcga gatggtcgat tattcgcgtc acgccgtcct tctcggcagt agtggaatgg
     4801 cggagctacg ctttattcgt acccacggca gccgttttac tccctaggat tgcacactgt
     4861 ttaactggct ggcacgcatt atcactccgg ttctgcaatc atggctcaat gatgaagaac
     4921 aacaggtggc gctgcgtttg ctggagaaag atcgcgatca tcatcgggta ctggttgata
     4981 ttactaatgc agtgctgtca catcttgatc tcgacgatct gatcgctgac gtcgctcgtg
     5041 agatccatca ttttttcggt ctggcttcag tcagtatggt actgggcgag catcgaaaga
     5101 acgagaagtt tagcctgtgg tgcagcgatc tttctgcctc acattgtgcg tgtctgccac
     5161 gcaatatgcc tggcgacagt gtattgctga cacaaacgct acaaacccga caaccgacct
     5221 tgacgcaccg tgcagacgat ctgtttctct ggcaacgcga cccgttatta ctcttacttg
     5281 catctaacgg ctgcgaatct gcgctcctta taccgcttac ctttggcaac catacaccgg
     5341 gtgcattgtt gctggcgcat acctcttcca ctctctttag tgaggaaaac tgccagctac
     5401 tacaacacat agccgatcgc atcgctattg ccgttggcaa tgccgatgcc tggcgtagca
     5461 tgaccgattt gcaggaaagt ttgcagcaag aaaactacca gcttagcgag cagctccttt
     5521 cgaatctggg catcggtgac attatctatc aaagccaggc aatggaagac ctgctccagc
     5581 aggtagatat tgtagcgaag agcgacagta cggtgttgat ttgtggtgaa accggaaccg
     5641 gcaaagaggt gatcgccaga gcgctccatc aacttagcac gcgacgcgac aaaccgctgg
     5701 tcaaaatcaa ctgcgctgcc atccccgcca gtcttctgga aagtgagtta ttcggtcatg
     5761 acaaaggggc gtttactggt gcgattaata cacatcgtgg tcgttttgaa attgccgatg
     5821 gcggcacgtt gtttctcgat gaaattggcg atctgccgtt agaacttcag cctaaactgc
     5881 tgcgcgtatt gcaggaacgg gagattgagc gtctcggcgg gagtagaacg atcccggtga
     5941 atgtcagagt cattgccgcc accaaccgtg atttgtggca aatggttgaa gatcgccagt
     6001 ttcgcagcaa tctcttttat cgcctgaatg tcttcccatt ggaattgccg ccgctacgcg
     6061 accgtccgga agatatccct cttttagcaa aacatttcac gcaaaaaatg gcgcgccata
     6121 tgaatcgcgc aattgacgcc atcccgaccg aggcgctacg ccagttgatg tcgtgggact
     6181 ggccgggcaa cgtgcacgag ctggaaaacg tgattgagcg agcggtactg ctgactcgcg
     6241 gtaacagtct gaatttacat cttaatgtcc gacaaagccg tttactgccg acgctaaatg
     6301 aagattcagc gcttcgcagt tcaatggcgc agttgctgca cccgacgacg ccagagaatg
     6361 acgaagaaga acgtcagcgc attgttcagg tattgcgaga aaccaatggc attgttgccg
     6421 ggccccgtgg cgcggcgaca cgattaggga tgaagcgcac cacgctgctg tcacgaatgc
     6481 agcgtctggg gatctcggtt cgcgaggtgt tgtaatctgc ttttgcagga gtatgcatga
     6541 gaaacaaact ctctttcgac ttgcagttga gcgccagaaa agcggcaatc gctgaacgga
     6601 ttgccgtcca taaaattgcc cgcagtaaag tgtcggtctt tttaatggcg atgtccgctg
     6661 gcgtgtttat ggcgatcgga tttacttttt acctttccgt tatcgccgat gccccgtctt
     6721 cacaggcatt aacccatctg gtgggcggcc tttgctttac actcggcttt attttgctgg
     6781 cggtttgcgg cacagcctgt tcacctcgtc ggtaatgacg gtgatagcaa aaagtcgggg
     6841 cgttattagt tggcgaactt ggctgattaa cgcacttctg gtggcctgtg gtaatctggc
     6901 agttattgcc tgtttcagtt tgttaatctg gttttccggg ctggtgatga gtgaaaacgc
     6961 gatgtgggga gtcgcggttt tacactgcgc cgagggcaaa atgcatcata catttactga
     7021 atctgtcagc ctcggcatta tgtgcaatct gatggtttgc ctggcgctgt ggatgagtta
     7081 ttgcgggcgt tcgttatgcg acaaaatcgt cgccatgatt ttgcccatca ccctgtttgt
     7141 cgccagtggc tttgagcact gtatcgccaa tttgtttgtg attccgttcg ccattgccat
     7201 tcgccatttc gccccccccc ttctggcaac tggcgcacag tagcgcagac aattttccgg
     7261 cactgacggt cagccatttt attaccgcca atctgctccc ggtgatgctg ggtaatatta
     7321 tcggcggtgc agtgctggtg agtatgtgtt atcgggctat ttatttacgt caggaatcct
     7381 gaaaaatcag cccggcgaaa cagttcgtcg ggctgaagat tattcttgcg cgatttgccc
     7441 atcgggccag gcgtggatca cggctttgat cagcgtcgcc aatggaatgg cgaagaatac
     7501 gccccagaat ccccacaaac caccgaagat caccaccgat aaaataatca ccagcggatg
     7561 caggttaacc gcttcggaga acaacaccgg tactagcagg ttgccgtcca gcgcctgaat
     7621 aatcagatac accgcgaagc agctccagaa ttccgtgcct gcgccaaact ggaatagcgc
     7681 cacgccaacc accggaatgg tcaccacaaa tgcgccaatg tacggaataa gaaccgagaa
     7741 gccgaccagc accgccagca gcagcgaata gttcagccca aagagcaaga accccagcca
     7801 ggtggcgatc cccaccacga tcatctccag cactttgccg cggatatagt tggtgatttg
     7861 ttgattcatc tccttccaca cctgtcctgc cagtccacgg ttgcgcggca gcacccgacg
     7921 aacggcgttc agcatctgct ctttgtcttt cagcaggaag aagaccatca atggcaccag
     7981 caccagatag acggcaatgg tcagcaaacc gaccagcgag gcgagggaaa ttttcaccac
     8041 cgaatcgccc atggtcagca tccgactgcg catattttcg gccattgcat caataatgcc
     8101 cgcgtccatt aacgccggat agcggcgcgg caacgtggcg gcaaagtcag aaagcttatt
     8161 gagcatcccc ggcatatcgc ggattaagta gatgccctgt tgccaggcga taggcagtac
     8221 cacgaacgcc atcagtagca atataccgac aaaaaccacc aatacaatcg acgtcgccca
     8281 gcggcgggag cagccaatag attgcaggcg cacggttggc cattccagca aataggccag
     8341 cacaatagcc accagcaacg gagcaagcag gccactaaag aaaaagataa tgccaaatcc
     8401 ggcaactaaa ataaccagca aggcaatcgc ttccgggtcg ctaaaacggc ggcgatacca
     8461 ttgcatcaac atttcgagca taaaaacctt ccctgaacct caagagcggg attgcgatcc
     8521 gcaattgtat cgaaatgtca caaaaaagac ttcgcttttt atgacggatt caggaaactg
     8581 aaaagtcatt tgagtgggct aatcttcgcc gttacactca aaggcggcgc ggtgggaacg
     8641 atatttcaca gtatcggtca aatgactatc ttcagaaata caggatagag gttatgttca
     8701 ggcagttgaa aaaaaacctg gttgcaaccc tcattgctgc tatgaccatt ggtcaggtag
     8761 ccccggcgtt tgccgacaac gcagacacct tgccggatat gggaacctcc gcaggaagca
     8821 cgctttccat tggtcaggaa atgcagatgg gcgactatta tgtccgccag ctacgcggca
     8881 gcgcgccgtt aattaatgac ccgctgttaa cgcaatatat taattcgctg gggatgcgtc
     8941 tggtttcgca tgccaattcg gttaagacac cgtttcattt ttttctaatc aacaacgacg
     9001 aaattaacgc ctttgctttc tttggcggca acgtggtgct gcactctgcc ctgttccgtt
     9061 attccgataa cgaaagtcaa ctggcttcag ttatggcgca cgaaatctcc cacctcaccc
     9121 aacgtcacct ggcgcgagcg atggaagatc agcagcgcag cacgccgctg acctgggtcg
     9181 gcgcgttagg ttctatttta ctggcgatgg ccagtccgca ggcggggatg gcggcgctga
     9241 ccggtacact ggcgggaacg cgtcagggga tgatcagttt cacccagcaa aatgaacagg
     9301 aagcggaccg cattggtatt caggtgctgc aacgctcggg attcgatccg caggcgatgc
     9361 caaccttcct cgaaaaatta ctcgatcagg cgcgttactc ctcgcgcccg ccggaaattt
     9421 tattgactca cccgttgccg gaaagtcgtc tggcagatgc ccgcaaccgt gctaatcaga
     9481 tgcgaccgat ggtggtgcag tcgtcggaag atttctatct ggcgaaagcg cgcacactgg
     9541 ggatgtataa ttccggacgt aaccagctca ccagtgattt gctggatgaa tgggcgaaag
     9601 gaaacgttcg tcagcaacga gcggcgcaat atggtcgtgc tttacaggcg atggaagcca
     9661 ataaatacga cgaggcgcgt aaaacgctgc aaccgttact ggcggcagaa cctggcaacg
     9721 catggtatct cgatctggct accgatatcg atcttgggca aaacaaagcc aatgaggcaa
     9781 tcaatcgcct gaaaaatgcc cgtgatttgc gcaccaatcc ggtgttgcag ctcaacctgg
     9841 caaacgctta tctgcaaggc ggtcaaccac aagaagcggc caatattctc aatcgctaca
     9901 cctttaataa taaagatgac agcaacggct gggatttact ggcacaggcg gaagccgcgc
     9961 taaataaccg cgatcaggag ctggctgcgc gagcagaagg ttatgcgctc gccggacgac
    10021 tcgatcaggc catttcgctg ttgagtagcg ccagttcgca ggtgaaatta ggcagcctgc
    10081 aacaagcgcg ttacgatgcg cgcatcgacc agttgcgcca gctgcaggaa cgctttaagc
    10141 cttataccaa gatgtaattc gcattaaagg aagaacaatg accaaacagg taaaaatcta
    10201 ccataaccca cgctgttcaa agagccggga aacgctgaac ctgctgaaag aaaacggcgt
    10261 ggagccagaa gtggtactct accttgagac acccgccgat gcggcaacgc tgcgcgattt
    10321 gctgaaaatg ctggggatga atagcgcccg tgaattgatg cgccagaaag aggatcttta
    10381 taaagaactg aacctggcta acagttcgct tagcgaagag gcgctgattc aggcaatggt
    10441 ggataatccg aagctgatgg agcgcccgat tgtggtcgcg aatggcaaag cgcggattgg
    10501 tcgtccaccg gagcaggtac tggaaatcgt gggttaagat gtgctccatc gctagttgaa
    10561 gcacattgcc ggatgcgacg cttgccatgt cttatccggc ctacgaatac tatggattaa
    10621 cttcgcgtag ttcggataag gcgttcgcgc cgcatccgac aataaacacc ttatctacaa
    10681 cttcagaatt tctttcacaa acggaatggt cagcttacgt tgtgcggtaa tcgacgcacg
    10741 atccaactga tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc gcttcagcaa
    10801 gaaacgcccc acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta actgtagcgc
    10861 ctgcaactta tcttcatcag aaagtggctg caatttgtag atctgccccc agtcgagtcg
    10921 cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc cggtgatcaa
    10981 caaccgtgtt ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg ccatctccca
    11041 caactcatcg cctgcaatac actcaatgtt gtcgatacaa accagcgaca aatgctccat
    11101 accgtcgagc acttccggaa caaaccaagt gcgtttatcc agcgggacat agcccaccgc
    11161 atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc gccccgcgcc
    11221 ttcgcgtgcc cagagataga tgtaaccgct atgttcctga cgcagcacgt tttgcagcgc
    11281 ggccagtaaa gaggagttat cccccggcca gaaacttgca aaggtttcgt cgtcaggaag
    11341 ataaagtggc aaagagagct gtgccggtgt gttcagagat acctcaacca ggatttcaca
    11401 aaatcgcgag aagtttacca cagaatccca tgatgtttga accgggcagc aacactgccc
    11461 ggtcggtaca ttatttgtct gttatatccg cctcttctgc gtccagcacc acttcttcag
    11521 ggcgcagcac actgatcagt ttgaaaatca aacttaagcc gataccgacg atggtcgcca
    11581 gcgccatacc ttttaactcc gccgcgccga tgtttacctt cgcgccactg acgccgatga
    11641 tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt gtaatccact ttcgattcga
    11701 tcaaaacacg aataccggaa gcaccgatga caccataaag cagcagcgaa acgccgccca
    11761 tcaccggcaa tgggatcatc tggatagcgg cagccagttt accgacgcag gaaagcagga
    11821 tagcgaaaat cgccgccccg ccgataaccc aggtactgta aacacgggtg atcgccatca
    11881 cgccaatgtt ttctccgtaa gtggtatttg gcgtggagcc aaagaagccg gagatcacgg
    11941 tcgacaggcc gttagcaaac atcgaacggt gcagacctgg atcgcgcagc agatcttttt
    12001 tgacgatatt agccgttact accaggtgcc ctacgtgttc ggcaataacc actaacgccg
    12061 ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg gttggcagcg
    12121 caaaccagtg agcattaata atcggcgtgg tatcgacaat tcccattgcg aaagagagcg
    12181 cgtagcccac cagcacgcca attaaaatcg ggataattgc caggaaacca cgaaacagca
    12241 cggaacctaa aaccgtgacc gccagggtgg taatagagat gatgatggtt ttggagtctg
    12301 gcgtttgccc ttcagccggg agtaaacccg ccataccggc agctacgccc gccagctcca
    12361 gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg aaacagcacg tccagccagc
    12421 cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg cacataataa
    12481 agccgcccag cgcgacttca taccctaacg gcaacagtaa caataccggt gaaataaagg
    12541 caaagctgga accaagataa gccggaattt tccctttaca gatgaagaga tacagcagcg
    12601 ttccaatacc gttaaataac agtacagtcg ccgggttaat atgaaataag acgggcacca
    12661 ggacggttgc accaaacatg gcgaacaaat gttgcaaact aagcgggatt gtctgtaaaa
    12721 gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat agtattatcc tctgtattat
    12781 gtgttatagg cgctttactc aaaaaaaagc cgactcttaa agtcggcttt aattattttt
    12841 attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg aataatgtat
    12901 ccgtgctcgt tcagtccctg atcaatcgat gcggtataca gttcgacgtc cgggtgcgct
    12961 ttttccagcg cagcgatacc ttctggcgca gcgaccagca ccagaacttt gatgctgctg
    13021 cagcccgctt ttttcagcag gtcgatggtc gcgataacgg aaccaccggt tgccagcatt
    13081 gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg gaagtacggt
    13141 accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg cgcgctcgga
    13201 acattttcca gcacaccgtc catcatacca agacccgcac gcagaattgg cacaacggta
    13261 attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc gatagttact
    13321 ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac ttcggaagcg
    13381 agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag cttgtgtttg
    13441 acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg ggcagccaca
    13501 aaaaaatcga cggattatac ctcctttctt caaggcggca atattctttt cgttgacttt
    13561 agtcaaaatg ataacgattt gagataaagt tattttatat tcaaatggtt atgaaagaag
    13621 atcattccat ccgaaaacta acctttgccc tggcacaagt cttctttcgc cgcgcgtctg
    13681 gggaaaagac gtgcaaaaag gttgtgcaaa gcagcctcgc aaacgtttgc tttccctgtt
    13741 agaattgcgc cgaattttat ttttctaccg caagtaacgc gtggggaccc aagcagtgac
    13801 cgataaaacc tctcttagct acaaagatgc cggtgttgat attgacgcgg gtaatgctct
    13861 ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct
    13921 gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc
    13981 cggcactgac ggcgtaggta ccaagctgcg tctggcaatg gacttaaaac gtcacgacac
    14041 cattggtatt gatctggtcg ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc
    14101 gctgtttttc ctcgactatt acgcaaccgg aaaactggat gttgataccg cttcagcggt
    14161 gatcagcggc atcgcggaag gttgtctgca atcgggctgt tcactggtgg gtggcgaaac
    14221 ggcagaaatg ccggggatgt atcacggtga agattacgat gtcgcgggtt tctgcgtggg
    14281 cgtggtagaa aaatcagaaa tcatcgacgg ctctaaagtc agcgacggcg atgttctgat
    14341 tgcactcggt tccagcggtc cgcactcgaa cggctattcg ctggtgcgca aaattcttga
    14401 agtcagcggt tgtgatccgc aaaccaccga acttgatggt aagccattag ccgatcatct
    14461 gctggcaccg acccgcattt acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt
    14521 gcatgccatt gcgcacctga ccggcggcgg cttctgggaa aacattccac gcgtattgcc
    14581 agataatact caggcagtga ttgatgaatc ttcctggcag tggccggaag tgttcaactg
    14641 gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt
    14701 cgggatgatt attgccctgc ctgctccgga agtggacaaa gccctcgccc tgctcaatgc
    14761 caacggtgaa aacgcgtgga aaatcggtat catcaaagcc tctgattccg aacaacgcgt
    14821 ggttatcgaa taatgaatat tgtggtgctt atttccggta acggaagtaa tttacaggca
    14881 attattgacg cctgtaaaac caacaaaatt aaaggcaccg tacgggcagt tttcagcaat
    14941 aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc
    15001 atcgccagcg cgtttgacag tcgtgaagcc tatgaccggg agttgattca tgaaatcgac
    15061 atgtacgcac ccgatgtggt cgtgctggct ggttttatgc gcattctcag cccggcgttt
    15121 gtctcccact atgccgggcg tttgctgaac attcaccctt ctctgctgcc gaaatatccc
    15181 ggattacaca cccatcgtca ggcgctggaa aatggcgatg aagagcacgg tacatcggtg
    15241 catttcgtca ccgatgaact ggacggtggc ccggttattt tacaggcgaa agtcccggta
    15301 tttgctggtg atacggaaga tgacgtcacc gcccgcgtgc aaacccagga acacgctatt
    15361 tatccactgg tgattagctg gtttgccgat ggtcgtctga aaatacacga aaacgccgcg
    15421 tggctggatg gtcaacgtct gccgccgcag ggctacgctg ccgacgagta atgcccccgt
    15481 agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc gcaagctcca
    15541 gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc tcgccataat
    15601 atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca ggaaaagcta
    15661 tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca ggaagcggcg
    15721 gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc caataacctt
    15781 gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat tagcgaagaa
    15841 caaggctcca actctcattc ccgccattta ctgggcaaaa ttcagtcccg ggtgctgaaa
    15901 gccgatcagg aattcgacgg cctctacaac gagctattgc tggagatggc gcgcaaccag
    15961 atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct tcgtcattat
    16021 tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga cactgactta
    16081 gtgcagttcc tgaaggatga ttacacctat ctggcggtgg aaattatccg tggagacacc
    16141 atccgttacg cgctgctgga gatcccatca gataaagtgc cgcgctttgt gaatttaccg
    16201 ccagaagcgc cgcgtcgacg caagccgatg attcttctgg ataacattct gcgttactgc
    16261 cttgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta ttcaatgaag
    16321 atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct gatggagttg
    16381 atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt ttatcagcgc
    16441 gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc ccgctacgac
    16501 tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt ccccaatgtc
    16561 ggcaaagcca atctggtgaa caaaccactg ccgcgtttac gccacatttg gtttgataaa
    16621 gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg atgtgttgct ctattatcct
    16681 tatcacacct ttgagcatgt gctggaactg ctgcgtcagg cttcgttcga cccgagcgta
    16741 ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt cacgcatcat cgactcgatg
    16801 atccacgccg cacataacgg taagaaagtc actgtggtgg ttgagttaca ggcgcgtttc
    16861 gacgaagaag ccaacattca ctgggcgaag cgcctgaccg aagcaggcgt gcacgttatc
    16921 ttctctgcgc cggggctgaa aattcacgcc aaactgttcc tgatttcacg taaagaaaac
    16981 ggtgaagtgg tccgttacgc acacatcggg accgggaact ttaacgaaaa aaccgcgcgt
    17041 ctttatactg actattcgtt gctgaccgca gatgcgcgta tcaccaacga agtacggcgg
    17101 gtatttaact ttattgaaaa cccataccgc ccagtgacat ttgattattt aatggtgtca
    17161 ccgcaaaact ctcgccgtct gttatatgaa atggtagacc gcgaaatcgc caacgcgcag
    17221 caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa aggcctggtt
    17281 gatcgtctgt atgcggcctc cagctccggc gtaccggtta atctgctggt tcgcggaatg
    17341 tgttcgctga tccccaatcc ggaaggcatt agcgacaaca ttcgtgccat cagtattgtt
    17401 gaccgttacc ttgaacatga ccgggtttat atttttgaaa atggcggcga taaaaaggtc
    17461 tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga agtggcgaca
    17521 ccgctgctcg atccgcacct gaagcagcgg gtgctggaca tcatcgacat attgttcagc
    17581 gatacggtca aagcacgtta tatcgataaa gaactcagta atcgctacgt tccccgcggc
    17641 aatcgccgca aagtacgggc gcagttggcg atttacgact acatcaaatc actcgaacaa
    17701 cctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt ttgctgcggt
    17761 cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg gtgccatgca
    17821 gattattggc cgcctgaaac agcgagtgca tctggcggac ggcctggggc cagataatat
    17881 gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctgtttg ccgaacggct
    17941 acaagggttt tctcctgcca gcgtctgtat agtcggcacc catacgctgc gtcaggcgct
    18001 gaacgccact gactttctga aacgcgtgga aaaggtcatt ccctacccga ttgaaattat
    18061 ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc aaccggagaa
    18121 aggtcgcaaa ctggttattg atattggcgg cggatctacg gaactggtga ttggtgaaaa
    18181 tttcgaaccg attctcgttg aaagccgccg gatgggttgt gtcagctttg cccagcttta
    18241 cttccctggc ggggtcatca ataaagagaa ttttcagcgc gctcgtatgg cggcagcaca
    18301 aaaactggaa actttaacct ggcaattccg tattcagggc tggaacgtgg caatgggcgc
    18361 ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga aagacgggat
    18421 aattaccccg gaacgtctgg aaaaactggt aaaagaagtt ttacgtcacc gtaatttcgc
    18481 atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca gtcttcgttc ctggactggc
    18541 gattttatgc ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt ctgacggggc
    18601 gttacgcgaa ggcgtactgt acgaaatgga aggacgtttc cgtcatcagg atgtgcgtag
    18661 tcgcaccgcc agcagcctcg ccaaccagta tcacatcgac agcgaacagg cccgacgggt
    18721 gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga agctggcgca
    18781 tccgcaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg ggttgaatat
    18841 caaccacagc ggtttgcatc gccactccgc ttatattctg caaaacagtg acttgccggg
    18901 ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc gcaaagcgat
    18961 taaactcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc tgccactgat
    19021 tcagctattg cgccttggcg tattactcaa caatcaacgt caggcaacca ccacaccgcc
    19081 aacattgaca ctgattactg atgacagtca ctggacactg cgtttcccgc atgactggtt
    19141 tagtcagaat gcgttggtac tgcttgatct ggaaaaggag caagaatact gggaaggcgt
    19201 ggctggctgg cggttgaaaa ttgaagaaga aagtacacct gaaatcgccg cttaatgctt
    19261 tgcgggccga cgagaaatgt cggcccgcat tattcaggca ctttcgcgaa tgggttcg
//
LOCUS       DAJTYD010000066        19068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTYD010000066 DAJTYD010000000
VERSION     DAJTYD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.066"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..750)
                     /locus_tag="OO585_002068"
     CDS             complement(<1..750)
                     /locus_tag="OO585_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6629661.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNN"
     gene            1093..1923
                     /locus_tag="OO585_002069"
     CDS             1093..1923
                     /locus_tag="OO585_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6629662.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            1955..2890
                     /locus_tag="OO585_002070"
     CDS             1955..2890
                     /locus_tag="OO585_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6629663.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2983..3225
                     /locus_tag="OO585_002071"
     CDS             2983..3225
                     /locus_tag="OO585_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6629664.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3215..4666
                     /gene="panF"
                     /locus_tag="OO585_002072"
     CDS             3215..4666
                     /gene="panF"
                     /locus_tag="OO585_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6629665.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFY
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            4678..5559
                     /gene="prmA"
                     /locus_tag="OO585_002073"
     CDS             4678..5559
                     /gene="prmA"
                     /locus_tag="OO585_002073"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6629666.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            5888..6853
                     /gene="dusB"
                     /locus_tag="OO585_002074"
     CDS             5888..6853
                     /gene="dusB"
                     /locus_tag="OO585_002074"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6629667.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            6879..7175
                     /gene="fis"
                     /locus_tag="OO585_002075"
     CDS             6879..7175
                     /gene="fis"
                     /locus_tag="OO585_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6629668.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7261..8145
                     /gene="yhdJ"
                     /locus_tag="OO585_002076"
     CDS             7261..8145
                     /gene="yhdJ"
                     /locus_tag="OO585_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6629669.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8229..8408
                     /locus_tag="OO585_002077"
     CDS             8229..8408
                     /locus_tag="OO585_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6629670.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8411..9073)
                     /gene="acrS"
                     /locus_tag="OO585_002078"
     CDS             complement(8411..9073)
                     /gene="acrS"
                     /locus_tag="OO585_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6629671.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            9472..10629
                     /gene="acrE"
                     /locus_tag="OO585_002079"
     CDS             9472..10629
                     /gene="acrE"
                     /locus_tag="OO585_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6629672.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10641..13745
                     /gene="acrF"
                     /locus_tag="OO585_002080"
     CDS             10641..13745
                     /gene="acrF"
                     /locus_tag="OO585_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCR6629673.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            13998..14219
                     /locus_tag="OO585_002081"
     CDS             13998..14219
                     /locus_tag="OO585_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629674.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIAMSK"
     gene            14650..15693
                     /locus_tag="OO585_002082"
     CDS             14650..15693
                     /locus_tag="OO585_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6629675.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTALQSG
                     EVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAG
                     TDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNP
                     AEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPA
                     TPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNL
                     WNNGGIQYAPPVR"
     gene            15761..16931
                     /locus_tag="OO585_002083"
                     /pseudo
     CDS             15761..16931
                     /locus_tag="OO585_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            16941..18044
                     /locus_tag="OO585_002084"
     CDS             16941..18044
                     /locus_tag="OO585_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6629676.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            18052..18809
                     /locus_tag="OO585_002085"
                     /pseudo
     CDS             18052..18809
                     /locus_tag="OO585_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
BASE COUNT         4672 a         4549 c         4796 g         5051 t
ORIGIN      
        1 attgttgtag aaatatcctt ttaacgcatc ggcaaacgca attttcaatt ctgtcgcgac
       61 attcacttta caaataccgc ggcgaataca gtgctgaacg tcctgttctg ataagcccga
      121 agcaccatgc aacaccagtg gaatctccac tcgctgacga atctgttcca gccgcacaaa
      181 atccagtcgt ggttcatgcg catataaacc atgtgctgtg ccgattgcta cggccaggga
      241 gtcaatttgg gtacgttgaa caaactctgc agctgcggct ggtgaggtat aagcggaatc
      301 tttttcatcc accagcagat cgtcctcctg gccgccaaga cgccctagct cggcctcaac
      361 gctaacatca aagcgatggc aaaaggtggt aacttcttgc actcgcgcaa tgttgtcttc
      421 aaatggcaaa tgggagccat caatcatgac agaacggatc cctgcgcgaa ccttgttaaa
      481 aatgtcatca ctctcttcat gatggtcgag atgcaggact aacggcaggt tgtactcgcg
      541 agccagcgat tgtactaacg aaattaaact tcccacgcca ccataacgat aagtaccggg
      601 tgttcccgcc agcataacgg gagagcgaag ttgtgcagcg ctctcaacaa ctacctgtaa
      661 agtttcaagg ttatgaatgt tgaaagcggg gacggcatat ccctctgact gggctttttt
      721 cagcatgttt ttgcttgaaa tgatgtacat cgcagctcct gttatttgtc gttcgcctta
      781 caaaatgaag aaagagaata ttcactttca ttttgaaagc aaactagcac agcaaaagcg
      841 gcaaaaagcg tgatacatgt cgaatatcat caaaataact tacaaaaaaa taacttaatt
      901 tgtttttcac tttacaagtg aaaccaataa catctctgca taaatatgaa taaattcatt
      961 tatttttcaa tgaattaatc tatttttgtt gcgatttttg acccatgatt ttcttttcga
     1021 attgtcgata ttattgaagg ataactttca aagaaaaata caaggggcta tagtgttacg
     1081 cattaaaggc caatgaagag tgttaacgtg ccgagaaaat cgtctaacct actgaaacgc
     1141 agattaaaaa ttgcagaaat tgttgccgcc caaggtgaaa tcaaagttga tgacttatct
     1201 gcgcaacttg gtgtctctgg cgtgacgatc cgcggcgatc tcagttatct ggagcaacag
     1261 ggatatttaa agcgatcatt tggcggtgct attgcgacac cactctcctc tgaaccagaa
     1321 gcgatacaac cgatcgcgcc attgtctctg gcacaacaaa tggaaatagc acgacattgc
     1381 gcacggttaa ttcgcgatcg tgacacgcta tttcttggac atgggacaat ctgccgtaag
     1441 atcattccat tgctcagcga agtgaaaaaa ctgcgtctga tcaccaacga tcccgaacat
     1501 gcgctgctgg caaatcagtt tattgatggc gaaataatac tggctggcag tgaaatgctg
     1561 cgccccgaca ccgcactggc cggaaaaccg cttgaacagg cacttcagca tttcaccatt
     1621 acacatagca ttctggaaat caaccatctg gatgctgatg gtactttaaa tatcaacgtt
     1681 cccagactgg ccgctgcctg gcagctctgt ttcgatcatg cccagaataa gacggttatc
     1741 gttgccgccg atcctgcgcc atcaacggct gctatcgggc atttgcatca ggcagaaaat
     1801 gtgatcgtca gccgcagcat taacgaacaa tatcagcaac aattgcagtc ggctgatttc
     1861 gttatccttt ataccagtaa tgagtgcttt acctggtcca atcgcgaaag gcttcaggaa
     1921 tgagaggggt tttgtgacaa taactatctg cgctatgggc gaattgctgg ccgagttttt
     1981 gtcccgcaac ccacatcaaa aattcactca gcctggggag tttatcgggc catttcccag
     2041 cggtgcgcca gcaatttttg ctgctcaggt ggcaaaactg tcccatcggg ccatcttctt
     2101 cggatgtgtt ggtaatgatg attttggccg actcattata gagcgcctcc gtcatgaagg
     2161 tgtcattacc gatgggatcc atgttatgaa caatgccgtc acaggtacgg cgttcgtgag
     2221 ttatcaaaat ccccagcagc gcgatttcgt ctttaatatc cctaacagcg cctgcggctt
     2281 gtttactgct gagcacattg ataaggatct gctaaaacag tgtaaccatc tccatattgt
     2341 gggctcatca ttgttctcat ttcgcatgat cgatgtcatg cgtaaagcaa taacgacgat
     2401 caaatcggct ggcggcaccg tttctttcga tcccaatatt cgcaaagaga tgctgagcat
     2461 tcctgaaatg gcgcaggctc tcgattattt gattgaatat acggatattt tcatccccag
     2521 cgaaagcgaa ctccctttct tcgcgcgtca caaaaatctg tcagaagaac agattgttag
     2581 cgatcttctc cacggcggcg taaaacatgt ggcgataaaa cgcgcccagc gtggggccag
     2641 ctattacaag cttaaaaacg gtacattaca cgcccagcat gttacaggcc acgatatcga
     2701 aattatcgat ccaacgggtg caggcgactg ctttggcgca acgtttatca ctcttttctt
     2761 atccggtttc ccggcacata aggcactgca atatgcaaat gccagcggcg cgctcgccgt
     2821 aatgcggcaa ggtccgatgg aagggatatc ctcactggct gacattgaag actttttgca
     2881 gcagcactga acggcaattt tctgtcagat tattttccct gcctgaagag ttgccgtaga
     2941 attgcccgtt tctcgtatcc ccccatgagg aggaaccctt ccgtggacac tcgttttgtt
     3001 caggcccata aagaggcgcg ctgggcgctg gggctgaccc ttttgtatct ggcagtttgg
     3061 ttagcagccg cttacttacc tgacgttgcc cccggtttta ccggctttcc gcgctggttt
     3121 gagatggcct gcatcctgac gccgctgctg tttattggac tgtgctgggc gatggtgaaa
     3181 tttatctatc gcgatatccc actggaggat gacgatgcag cttgaagtga ttctaccgct
     3241 ggtcgcctat ctggtggtgg tgttcggtat ctctgtttat gcgatgcgta aacggagcac
     3301 cggtaccttc cttaatgagt atttcctcgg cagccgctct atgggcggta ttgtgctggc
     3361 gatgacgctc accgcgacct atatcagtgc aagttcgttt atcggcgggc ctggagctgc
     3421 ttataaatac ggcctgggtt gggtattgct ggcgatgatt cagcttcctg cagtctggct
     3481 ttcgctcggt attctcggca agaagttcgc gattcttgcg cgccgctaca atgcagtgac
     3541 gctgaacgac atgctgtttg cccgctacca gagtcgtctt ctggtgtggc tggcgagttt
     3601 gagtttgctg gttgcgttcg ttggggcaat gaccgtgcag tttatcggcg gtgcgcgcct
     3661 gctggaaacc gcggcgggta ttccttatga aaccgggctg ctgattttcg gtatcagcat
     3721 tgcgttgtat accgcctttg gcggctttcg cgccagcgtg ctgaacgaca ccatgcaagg
     3781 gcttgtgatg ctgattggca ccgttgtgct gcttattggc gtggtacatg ccgcaggtgg
     3841 tttaagtaat gcggtagaga cgctgcaaac catcgatccg caactggtta cgccacaagg
     3901 cgctgacgat attctgtcgc ctgcctttat gacgtcgttc tgggtactgg tgtgctttgg
     3961 cgtgattggc ctgccgcata ctgcggtgcg ctgtatctct tataaagaca gcaaagcggt
     4021 acaccggggg atcatcatcg gtacgattgt agtcgcaatc ctgatgttcg gtatgcacct
     4081 ggccggagcg ttaggtcgtg cagtgatccc cgatctcacc gtaccggatc tggtgatccc
     4141 gacgttaatg gtaaaagtgc tgccaccgtt tgctgccggg atctttctgg ctgcacctat
     4201 ggctgcgatc atgtcgacaa ttaacgccca attgctacaa agttccgcta cgatcattaa
     4261 agatctctat ctgaatatcc gtccggatca aatgcaaaac gagacgcgtc tgaagcggat
     4321 gtcggcggta attacgttag ttctcggcgc gttgctgctg cttgccgcct ggaagccgcc
     4381 agaaatgatc atctggctga atttgctggc cttcggtggg ttggaagccg ttttcctgtg
     4441 gccgctggtg ctgggtcttt actgggaacg cgccaacgcc aaaggcgcgc taagtgcgat
     4501 gatcgttggc ggcgtgctgt atgccgtact cgcgacgctg aatattcagt acctgggctt
     4561 ctaccctatc gtgccctcgt tactactaag tttgctggct ttcctggtcg gaaaccgttt
     4621 cggtacatcc gtcccgcaag ctaccgtttt gactactgat aaataaagag ttttgccatg
     4681 ccttggatcc aactgaaact gaacaccacc ggcgcgaacg ctgaagatct tagcgatgcg
     4741 ctgatggaag cgggtgccgt ttctatcact tttcaggata cccacgatac gccagtattt
     4801 gagccgctgc cgggcgaaac gcgcctgtgg ggcgacaccg atgtgattgg tctgttcgac
     4861 gctgaaaccg atatgaacga cgtggtggcg attctggaaa accatccgct gctcggcgca
     4921 ggcttcgcgc ataaaatcga acaactggaa gataaagact gggagcgcga atggatggat
     4981 aatttccacc cgatgcgctt tggtgaacga ctgtggatct gcccaagctg gcgtgatgtg
     5041 ccggacgaaa acgccgtcaa cgtgatgtta gacccagggc tggcgtttgg tacgggtacc
     5101 catccaacta cctctctgtg cctacaatgg ctcgacagcc tcgatttaac tggtaaaaca
     5161 gtcatcgact ttggctgcgg ttccggcatt ctggctatcg cggcgctgaa actgggtgca
     5221 gcaaaagcca ttggtattga tatcgatccg caggcgattc aggctagccg cgacaacgcc
     5281 gaacgtaatg gcgtttctga ccgtctggaa ctctacttac cgaaagatca gccagaagaa
     5341 atgaaagccg acgtggtggt cgcgaacatc cttgcaggcc cattacgtga actggcaccg
     5401 ttaatcagcg tcctgccggt ttcaggcggt ttgctgggcc tttccggtat tctggcaagc
     5461 caggcagaga gcgtttgtga agcttatgcc gatagcttcg cactggaccc ggtcgtggaa
     5521 aaagaagagt ggtgccgtat taccggtcgt aagaattaac cttcgcatcg ccgtagggtg
     5581 acgcggggcg aagtgcgagc aagctcacaa aaggcacgta aatttgccgg ttatttacgc
     5641 aaatttgcgt gccaaaattt tcattcataa agaaaaattg agaacttact caaatttctt
     5701 tgaatgtaaa ttttagtcac tattttctaa tatgatgatt tttatgagta attatcgcac
     5761 cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa agtttggcct
     5821 ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta tggtcatttc
     5881 ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag cgcccatggc
     5941 tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg gattgacagt
     6001 atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc gtttacggat
     6061 ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg atccgaaaga
     6121 aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg atatcaatat
     6181 gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct tgcagtaccc
     6241 ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc ctgttaccct
     6301 gaagattcgc actggctggg caccggaaca ccgtaactgc gaagagattg cccaactggc
     6361 tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct gtttgttcaa
     6421 tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca ttccggttat
     6481 cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact atacaggggc
     6541 ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc gggaaatcca
     6601 gcattatctg gacactgggg agttgctgcc cccactgcct ttggcagagg ttaagcgctt
     6661 gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag ggtaccgaat
     6721 tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc agtttcggcg
     6781 cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg catacttcga
     6841 aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa cgcgtaaatt
     6901 ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa aaacccctgc
     6961 gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt caggatgtga
     7021 atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac atggtgatgc
     7081 aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac cgtggtacgc
     7141 tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa tgcttgatta
     7201 aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa gggagtgacc
     7261 atgagaacag gatgtgaacc gacccggttt ggtaatgaag ctaagaccat tattcacggt
     7321 gatgcgcttg ccgaacttaa aaagatcccc gccgaaagtg tcgatctgat ctttgccgac
     7381 ccaccatata acatcggtaa aaattttgat ggtctgatcg aagcctggaa agaagatctg
     7441 tttatcgact ggctgtttga agtgattgca gagtgccacc gcgttctgaa aaagcagggc
     7501 agcatgtaca tcatgaacag cacggaaaac atgcccttta tcgatctcca gtgccgcaag
     7561 ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttctggagt acaggcaaaa
     7621 aaacactacg gctccatgta cgaccccatc ctgatgatgg tgaaagacgc aaagaactac
     7681 acattcaacg gtgatgctat tctggtagaa gccaaaaccg gatcgcagcg cgcgttgatc
     7741 gattatcgca aaaatcctcc acagccatac aatcatcaaa aagtaccggg taacgtctgg
     7801 gattttccgc gcgtgcgtta tttgatggat gaatatgaga accacccgac gcaaaaaccg
     7861 gaagccttac tgaaacgcat tattctcgcc tcttccaacc caggcgatat cgttctcgac
     7921 ccgtttgccg gtagctttac taccggtgcc gtagccgtct ccagcgggcg aaaattcatt
     7981 ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga tgtcgcgtcg
     8041 cattactctg cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa cctgtcaaaa
     8101 cgaagccggt taagcgaagt tgaccccgat ctcattgcaa agtaaaggaa gtgtaagcct
     8161 ggttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa catgcacagg
     8221 ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc gtttttctgt
     8281 ttgatacttt actgatgcag tggattgaac tgctgacaac agaaacagac aaatgccgta
     8341 atatgaactc agttaatcca ctaaaactgg ttaactgtga cgaactgaat tttcaggaca
     8401 gaatgtgaat ttacatgaca cttaattcat tcgtttgatg aattaatttc gttatgtttt
     8461 catctggcat gaacattctt aatacgttat cgaccagcgc gggtgcttgt ttataaagat
     8521 cataacccgc catattcatt aaccagtttt gaacaattcc gctgaaggca ccattaataa
     8581 taatcatcac aacatctaaa tcgaggttat ttgctacaca accttgttgc tgacacgcct
     8641 gcaatacttc gcggagagtc tgcggattaa agcccatctt ttcgcgtatc actccctcgg
     8701 ccagcatctc atcattaaat tcacatttgt gatataagat tttcagcaac gcctgctggc
     8761 ggggaatttt ggcaatatat tgcaagccga caatcaattt ttcacgcaat tgttgaaacg
     8821 ggtcatgctc taatccagcc gtcaagtgtt cctggattaa ctcccgcaat gaaggctgtt
     8881 gcaaccacat ctcattaaac agttgagtct tgttttcgaa gtgccagtag atagcgccac
     8941 gcgtaacgtt agcggcgtcg gcaatgtcgt tgagcgtcgt cttgcttacg ccatgctgcg
     9001 caaactgggc gatggcagtt tcaatcagtt cttgccgggt cttcagagct tcggctttgg
     9061 ttctttttgc catgattaat tattcaggaa ataaatatat tcgacacagg gtgagaaaat
     9121 agcgaaggtt aatctatcat ctaatgtgta tttatacgag agggtaacat tgagttgcta
     9181 taaatcgtca aataaacaat gtatattatt tacctaaaat acattcgcca catgaatata
     9241 tatttcaatt tacgaggttt taattctgcc tctttcaacc cgcgtcaaaa taaaacagta
     9301 gaatattaat ctttttttgt gtttatgtgc cttgagacgc cagtattcat aactattcct
     9361 tacatcgacg aatgataatt tgtaggatag caaactgtat ttttctttct gcgagttaac
     9421 gcgttgcctt tctgggtaaa taacgcgctt ttggtttttt gaggaatagt aatgacgaaa
     9481 catgccaggt ttttcctcct gccctccttt attctgatct ccgcggcttt aatcgccggt
     9541 tgtaacgata agggagaaga gaaagctcac gtcggtgaac cgcaggttac cgttcatatt
     9601 gtgaaaacgg ccccgttaga agttaagact gaattaccag gccgcaccaa tgcttaccgt
     9661 atagccgaag ttcgcccaca ggttagcggg atcgtactga atcgtaattt cactgaaggc
     9721 agcgatgtac aagcaggcca gtccctgtac cagatcgatc ccgcgaccta tcaggcaaat
     9781 tataacagcg cgaaaggcga actggcgaaa agtgaagccg ccgccgccat cgcgcatttg
     9841 acggtaaaac gttacgttcc gctcgtgggt acgaaataca tcagccagca ggagtacgac
     9901 caggccattg ctgatgctcg ccaggccgat gccgccgtga ttgccgcaaa aggcacagtc
     9961 gaaagcgctc gcatcaatct cgcttatacc aaagtgactg cgccaattag cggacgtatc
    10021 ggcaaatcga ccgtgaccga aggcgctctt gtcactaatg ggcaaacgac tgaactggcg
    10081 actgtccagc agctcgatcc tatctacgtt gatgtgaccc aatccagcaa cgattttatg
    10141 aggctgaagc aatccgtaga gcaaggaaat ttgcataagg aaaacgccac cagcaacgta
    10201 gagttggtca tggaaaacgg tcaaacctat cccctgaaag gtacgctgca attctctgat
    10261 gtgaccgttg atgaaagcac cggctccata accctacgtg ctgtcttccc taacccgcaa
    10321 catacgcttt tgccgggtat gtatgtgcgt gcacggattg atgaaggcgt ccaacctgac
    10381 gccattctta tcccgcaaca aggcgttagc cgcacaccgc gtggtgatgc aaccgtgctg
    10441 attgttaacg ataaaagtca ggtggaagcg cgccctgtcg ttgccagtca ggcgattggc
    10501 gataaatggt tgattagcga aggtctgaaa tctggcgatc aagtcattgt cagcggcctg
    10561 caaaaagcgc gtccgggaga gcaggttaaa gccactaccg atacccccgc agatactgca
    10621 tcgaagtaag gtaatctgac atggcaaact tttttattcg acgaccgata tttgcatggg
    10681 tgctggccat tattctgatg atggcgggcg cactggcgat cctacaattg cccgtcgctc
    10741 agtatccaac cattgcaccg cctgcggttt ctgtttcggc aaactatcca ggtgctgatg
    10801 cgcagaccgt gcaggatacg gtgacgcagg ttatcgaaca gaatatgaat ggtatcgata
    10861 acctgatgta tatgtcctcc accaacgatt ccgccggtag cgtgacaatt acccttacct
    10921 tccagtccgg gaccgatcct gatatcgcgc aagtgcaggt gcagaacaaa ctccagctcg
    10981 ccacgccgtt gctgccgcag gaggttcagc agcaggggat cagtgttgaa aagtccagta
    11041 gcagctattt gatggtggcg ggttttgtct ctgataaccc aggcaccaca caggacgata
    11101 tctcggacta tgtggcctct aacgttaaag atacgcttag ccgtctgaat ggcgtcggtg
    11161 acgtacagct tttcggcgca cagtatgcga tgcgtatctg gctggacgcc gatctgctaa
    11221 acaaatataa actgacaccg gttgatgtga ttaaccagtt gaaggtacag aacgatcaga
    11281 tcgctgccgg acagttgggc ggaacgccag cgttaccagg gcaacagttg aatgcctcga
    11341 ttattgctca aacgcggtta aaaaatccgg aagaattcgg caaagtgacc ctgcgcgtaa
    11401 acagtgacgg ctcggtggta cgcctgaaag atgtcgcacg ggttgaactt ggcggtgaaa
    11461 actataacgt tatcgctcgt atcaacggaa aaccggcggc gggcctgggg attaagctgg
    11521 caaccggcgc gaatgctctc gataccgcga aagccattaa ggcaaaactg gcggaattac
    11581 agccattctt cccgcaggga atgaaggttc tctaccctta tgacaccacg ccattcgtcc
    11641 agctttctat tcacgaagtg gtaaaaacgc tgttcgaagc cattatgctg gtgttcctgg
    11701 tgatgtatct gttcttgcag aatatgcgag caacgctgat ccccaccatt gcggtacccg
    11761 tggtgttgtt agggacgttt gccatcctcg ccgcttttgg ttactccatc aacacactaa
    11821 cgatgttcgg gatggtgctt gccatcgggc tgctcgtcga tgatgcgata gtggtggtgg
    11881 agaacgtcga gcgcgtgatg atggaggata agctcccgcc aaaagaagcg acggaaaaat
    11941 cgatgtcgca aattcagggc gcactggtgg gtatcgcgat ggtgctgtca gcggtattta
    12001 ttccgatggc attcttcggc ggttctactg gggcaattta tcgccagttc tctatcacca
    12061 tcgtttcggc aatggcactt tctgttctgg tggcattgat tcttacccct gcgttatgtg
    12121 caacgctgct taaacccgtc tctgctgagc atcacgaaaa taagggcggt ttcttcggct
    12181 ggtttaatac caccttcgat catagcgtta accactacac caacagcgtc ggcaaaatcc
    12241 tcggttccac aggacgatat ttactgatct atgcgctgat tgttgcagga atggtggtgt
    12301 tgtttttacg tcttccgtct tccttcttac ctgaagagga tcagggtgtc tttctgacca
    12361 tgattcagtt acccgctggc gcgacgcaag agcggacgca aaaagtgttg gatcaagtga
    12421 cggattacta tctgaagaac gagaaagcga acgttgaaag tgtctttacg gttaacggct
    12481 ttagcttcag cggccaggca caaaacgccg gtatggcctt cgtcagtctg aaaccgtggg
    12541 aagagcgtaa tggtgatgaa aacagtgcgg aagcggtaat ccatcgtgcc aaaatggaat
    12601 tgggcaagat ccgcgacggt tttgtcattc cattcaatat gccagccatt gttgaactgg
    12661 gcacggcaac gggtttcgac tttgagttaa ttgatcaggc tgggctgggt cacgatgccc
    12721 taacccaggc ccgtaaccag ttgcttggta tggcggcgca acatcctgcc agcttagtca
    12781 gcgtgcgtcc taatggcctg gaagacaccg cgcagtttaa actggaagtt gaccaggaaa
    12841 aggcgcaggc attaagtgtt tcactttctg acatcaatca gaccatttca acggcgttgg
    12901 gtgggactta cgttaacgac ttcatcgacc gtggtcgcgt gaaaaaggtg tatgttcagg
    12961 cggatgccaa attccgtatg ctgccagaag atgtcgataa actttatgtc cgcagcgcca
    13021 acggcgaaat ggtgccattc tcggccttta ccacttcaca ttgggtgtat ggctctccgc
    13081 gactggaacg ctacaacggt ctgccgtcaa tggagattca gggggaagcc gcgccaggaa
    13141 ccagttccgg cgatgccatg gcgttgatgg aaaaccttgc gtcaaaatta cctgcgggca
    13201 ttggttatga ctggacgggt atgtcgtatc aggaacgctt atcgggaaac caggctcccg
    13261 ctctagtagc aatttccttt gtggttgttt tcctgtgcct tgctgcactc tatgaaagct
    13321 ggtcaattcc tgtctcggtt atgttggtcg tgccgttagg gattgtcggc gtgctgctgg
    13381 cggcgacact ctttaatcaa aaaaatgacg tctactttat ggtgggcttg ctaacgacaa
    13441 ttggcttgtc ggccaaaaac gctattttga ttgttgagtt cgctaaagat ctcatggaga
    13501 aagagggtaa aggtgttgtt gaagcgacac taatggcagt acgtatgcgt ctgcgtccta
    13561 tcctgatgac ctctctcgcc tttattctcg gcgtattacc gctagctatc agtaacggtg
    13621 caggcagtgg cgcgcagaac gcagtgggta tcggggtaat gggcggaatg gtctctgcaa
    13681 cgttgctggc aatcttcttc ctaccggtgt tctttgtggt gatccgccgt tgctttaaag
    13741 gataaatcag aaacataaag gcactttcgg gtgcctttat tattttcagt gaaacccata
    13801 aaaattaata agatattctt ccactcactc tttaaaaact ttctatagtt cccgctccct
    13861 tcactatttt tacaattcac ataatttgtc attattcatt ccgcaatatt tataacctcc
    13921 ggtgataaaa tggcattcag ctcgttaata agagagttaa cttattaagc gttagcgttt
    13981 attactgagg taacaccatg aaaagattaa ttcctgtggc attgctcacc gcattgctgg
    14041 cgggctgtgc tcacgattcg ccctgcgttc cggtttatga cgatcagggt tgtctggttc
    14101 acaccaatac ctgtatgaaa ggcacgactc aggataactg ggaaacggct ggggctattg
    14161 ccggcggggc agcggctgtt gctggcctga cgatggggat catcgctatg tcgaaataac
    14221 tgaacagggc gcggagtttc gcgctctgtt taacgataca gtatctttac ataagataaa
    14281 atattgctat attctgctgg ctaattctct gctgatttag catgttatat cgcgttattt
    14341 cgctgctttc gaaataacgc atcaatttaa ctttcacact tcccaccaga aatatactca
    14401 cgctaacaat cattattctg cttagtaaaa cacgcgtaaa tattttgcgc caaaatgtgg
    14461 cgcatgtttc attttcgcac cattgcgggg cgctgttttt attatttcct gtctacactc
    14521 agcttattgc gacgcgaaat cgtgcgcaaa agctggcact acttttgctt ataagaggat
    14581 ggccacagac aggtaaaaga cgtttcccaa acgtcctata acgataattt tcgcctcaca
    14641 ggaagcatta tgaaaaagat gatgatagcc acactggctg ccgccagcgt gctgcttgcc
    14701 gttgcaaatc aggcgcatgc tggcgcgacg cttgatgccg ttcagaaaaa aggttttgtg
    14761 caatgcggga tcagtgatgg attacttggg ttctcttatg ccgatgcaga cggtaagttt
    14821 tcaggtattg atgttgatgt tgatgttgat gttgatgttt gtcgtagtgt tgccgctgct
    14881 gtatttggtg acgacacgaa agtgaaatat accccactca cagcaaaaga acgcttcacg
    14941 gctttacagt caggggaagt ggatttgctc tcccgtaata cgacctggac ttcatctcgc
    15001 gacgccggga tgggaatggc atttactggc gtcacttatt acgacggcat tggcttcctg
    15061 acgcacgata aagcggggct aaaaagcgcg aaagaactgg atggcgctac cgtctgtatt
    15121 caggcgggta ctgataccga actcaacgtt gccgactatt tcaaggcaaa caatatgaag
    15181 tacacaccgg tgactttcga tcgctctgac gaatcagcga aggcactgga atctggtcgc
    15241 tgcgatacgc tggcctcgga tcagtcgcaa ctgtatgccc tgcgcatcaa attaagcaac
    15301 ccagctgaat ggattgtctt accggaggtt atctctaaag aaccgcttgg gccggtagtt
    15361 cgtcgtggcg atgatgaatg gttctcgatt gtacgctgga cgcttttcgc catgctgaat
    15421 gctgaagaga tgggcatcaa ttcccagaac gtcgatgaaa aagcggctaa tccagcaacg
    15481 cctgatatgg cacatctgct gggtaaagaa ggcgattacg gcaaggatct gaagttggat
    15541 aataaatggg cctataacat catcaaacag gtgggtaact actcggaaat tttcgagcgt
    15601 aacgtaggtt cagaaagccc gctgaaaatt aaacgtgggc aaaataatct ctggaataac
    15661 ggcggtattc agtacgcgcc gcccgtgcgt taagtcggtg tgaggtaacg agcgtcgcta
    15721 cggcggcgct ctcttcagcg ttatgctttc cgaggtttct atgtctcatc gccgctcaac
    15781 cgttaaaggc tcactctcct ttgccaaccc tacggttcgt gcctggttat tccagatcct
    15841 tgccgttgtt gctgttgtcg gaattgttgg ttggttattt cacaacactg taacgaatct
    15901 cagtaatcgt ggcattactt caggttttgc ctttctggat cgcggcgctg gcttcggtat
    15961 tgtccagcat ttgatcgatt accagcaggg cgacacctac ggacgcgttt ttattgtcgg
    16021 cttactcaat acgctactgg tttctgcatt gtgtattgtg ttcgcttctg ttttaggctt
    16081 ctttatcggt ctgacgagac tttcggataa ctgactgcta cgaaagctat ccacaattta
    16141 tattgagatc ttccgtaata ttcccccgct gctgcaaatc ttcttctggt actttgccgt
    16201 gttgcgcaat ttgcccggac cacgcgccag gctgtgagcg cgtttgatct ggcctttttg
    16261 agcaatcgtg ggctttatat tccgtcaccg cagctgggag acggatttat tgcgtttatc
    16321 ctagctgttg ttatggctat agtcctttct gttgggctat tccgctttaa taaaacatac
    16381 cagataaaga ccggacaact gcgccgcacc tggccgttcg ccgcagtgtt gatcatcggt
    16441 ttgcctttac tggcgcaatg gctttttggc gcagcactac actgggatgt cccagcccta
    16501 cgaggcttta acttccgcgg cggaatggtt ttaattcctg aactggcagc cttaacgctg
    16561 gcactttcgg tttatacctc tgcatttatc gccgagatta tccgcgctgg gatccaggca
    16621 gtgccttatg gtcaacatga agcggctcgg tcactggggt tacgctacgc caggtcatta
    16681 ttccccaggc actgcgagtg attattccgc cgttaaccag ccagtatctc aacatcgtca
    16741 aaaactcctc tcttgccgcc gctattggct atcccgatat ggtttcgctg tttgctggca
    16801 ccgtgctgaa ccagacgggg caagccatcg agacgatagc catgaccatg tcggtctatc
    16861 tgattatcag cctgactatc tcgctgctga tgaatatcta taaccgccgc atcgcgatcg
    16921 ttgaacgcta aggagtcatg atgacaaaag tattgctgtc tcagccctcg cgcccagcga
    16981 gccataactc aagccgcgcg atggtgtggg tgcgaaaaaa tctgttctcc agctggagca
    17041 atagcctgct gactattggc tgcatatggt tgatgtggga actgatccca ccgttgctga
    17101 actgggcatt tttgcaggct aactgggttg gctcaacgcg tgccgactgc acaaaagccg
    17161 gtgcctgttg ggtcttcatc cacgaacgat ttggtcagtt tatgtatgga ctttacccac
    17221 acgaccaacg ctggcgaatt aacctagtat tactgattgg ccttgtgtca atcgcaccaa
    17281 tgttctggaa aatactcccg catcgcggtc gctatattgc ggtctgggcg gtgatttacc
    17341 cactgattgt ctggtggctg atgtatggcg gtttttttgg tcttgagcgg gttgaaaccc
    17401 ggcaatgggg cgggctgacg ctaactttaa ttattgcatc cgttgggatt gcgggggcgc
    17461 taccgtgggg gatcttactg gcgttaggtc gccgctccca tatgccgatt gtgcgtatct
    17521 tatcggtcat ttttatcgag ttctggcgcg gcgtaccgct gattaccgta ttgtttatgt
    17581 cttcggtcat gctgccgttg tttatggcag aaggcaccag tatcgacaaa ctgatccgcg
    17641 cgctggttgg cgtgatcctg tttcagtcag catatgttgc ggaagtcgtg cgaggcggat
    17701 tacaggccct acctaaaggg caatatgaag cggcagagtc gctggcgttg gggtactgga
    17761 aaactcaggg gctggttatt ctgccacagg cgttgaagct ggtaattcct gggctggtaa
    17821 ataccatcat cgcactcttc aaagatacca gcctggtgat catcatcggc ttgttcgatc
    17881 ttttcagtag cgttcagcag gcaaccgttg atcccgcctg gttgggtatg tcgacggaag
    17941 ggtatgtttt cgccgcgctg atctactgga tcttttgttt cagcatgtca cgttatagcc
    18001 aacatctgga aaaacgtttt aacaccgggc gtacaccgca ttgaggacac tatgagccaa
    18061 attttactgc aacctgctaa cgcgatgatt acgctggaaa acgtccataa atggtatgga
    18121 caattccatg ttttgaaaaa tataaattta accgtgcaac cgggagaacg gatcgttctg
    18181 tgtggccctt ctggttccgg taaatcgaca accattcgtt gtattaatca tctggaagaa
    18241 catcaacagg gacggattgt cgtagatggc atcgaactta atgaagatat ccgcaatatt
    18301 gagcgcgtca ggcaagaagt gggaatggta tttcagcatt tcaatctctt ccctcatctg
    18361 accgttttac agaactgtac cctggcaccg atttgggtac gcaagatgcc taagaaagag
    18421 gctgaagctc tggcgatgca ttaccttgag cgggtgagaa ttgccgaaca tgcgcataag
    18481 tttcccggac agatttcagg tggtcagcag caacgcgttg ccattgcgcg ttcgctgtgt
    18541 atgaagccga aaattatgtt gtttgatgaa ccaacgtcgg cctcgatccg gagatggtga
    18601 aagaggtgct ggatacgatg attgggctgg cgcagtcggg tatgacaatg ttgtgtgtaa
    18661 cacatgagat ggggtttgca cgtaccgtcg ctgaccgggt aatttttatg gatcgtgggg
    18721 agatagtgga acaagctgca cctgatgaat tttttacgca tcctaaatca gagcgtacga
    18781 gggcattttt atcgcaggta atccattaat tgaatgttag ttcgaaaagc aaaaaggcca
    18841 tcctttcgga tggccttttt gcttgatttg atgtctggca gtttatggcg ggcgtcctgc
    18901 ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg gatttgtcct
    18961 actcgagaga gtgttcaccg acaaacaaca gataaaacaa aaggctcagt cggaagactg
    19021 ggccttttgt tttatttgat gtctggcagt tccctactct cgcatggg
//
LOCUS       DAJTYD010000067        18907 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTYD010000067 DAJTYD010000000
VERSION     DAJTYD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.067"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO585_002086"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO585_002086"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6629677.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO585_002087"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO585_002087"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6629678.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO585_002088"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO585_002088"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6629679.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO585_002089"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO585_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6629680.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO585_002090"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO585_002090"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6629681.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO585_002091"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO585_002091"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6629682.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO585_002092"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO585_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6629683.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO585_002093"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO585_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6629684.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO585_002094"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO585_002094"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6629685.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO585_002095"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO585_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6629686.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO585_002096"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO585_002096"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6629687.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO585_002097"
     CDS             complement(15665..16702)
                     /locus_tag="OO585_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6629688.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="OO585_002098"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="OO585_002098"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6629689.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRSSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17759..>18907
                     /locus_tag="OO585_002099"
     CDS             17759..>18907
                     /locus_tag="OO585_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCR6629690.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLSKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPP"
BASE COUNT         4825 a         5012 c         4602 g         4468 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattggtgaa cttacaatct caccggatcg atatgccaga
       61 tatgatcggc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gaacctgata gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgctag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accagaaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccttggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcaac gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagca cgagaccaca gtacaaaagc acgtcgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg gccggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagacccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggcg cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgccggaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaagttgt gcacagtaaa caccgacttc gccggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaaggc ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgtccg gcgaaggtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacttgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agctttcagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccggagtt
     5041 gatgcagtta gacagcgcaa agtcgataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacag ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccatcgc cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag gttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtaccctgg
     5701 gagagcaaca acgttgttaa caaggcgtga atactgtcgc gccgccgttc aaccaggtca
     5761 agagaaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcagt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accaccaggg gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtgacaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagtgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cggaatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcatcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaatt ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgtgccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagtg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgca tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccacc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcacggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg gaatgcccac atccagccat
     7741 agtaggcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagaat cccgaaggtg agtttatcgt gatgatactg acgataaacc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cgcgataaat cattgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca taattgtaga
     8281 tcagcgtatt ccagtcctga tgatagcctt cgcgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cggtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagacccgct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttatcg gtgtggcgac
     8641 gccaggagcc caggtgaact tcataaatag agattgacgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaaa
     8881 tgccgctctc tttacgcaga cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccaaacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcgggccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacacc gctaaagaat ccccgtgagt caagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcacccacgg taaaggctga caggaactcc ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac acagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaaccc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agaacccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cggagtagtt cagttgttca atgaccggca tcaccagccc caccgccgtt aaacaggcaa
    11881 cggtggcaga cccctgaatg atgcgcactg cagcggccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgta
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa tggatgacca ataaactcga
    12181 accattcgta agcagttgat ccttctggca caaaacgagc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaggacggc attttgcctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcaa ctccacgtaa cggctgatga
    12361 aattacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac agaccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag caacatcggc gctggtccag
    12481 gcaccagaaa tgccgccgct gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga actatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca accccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 gtaagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggggc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcaag cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac agacgatcag cgacacttta ttggtgcgca
    13261 gcatagcaaa cgcggcgtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcaccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtaggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gctaaccgcg gctccatcac ctggccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa ccttcaatcc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccaggtca tcgttggtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatacga caccagacct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgc gcgccgagat aggcaatgtg gcaatgcccg cgagcaataa
    14101 tggcggtggt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatg agttccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcagtcc gtcgatattc catgagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgagcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt ggcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca gcggtacagt
    15061 tccatatcct gatgcacttt caatgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgta cggcatcgaa gcgacgctgt tcataacgcg gcgtaatacc gttttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac gccctcgtca ttgacatact ccggcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccataaaac gcgccatcct
    16201 gcggcagccc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 caacaatctc tcccaacttg ccactttcaa tcgctttttt cgctgtcagg aagcaggagt
    16321 caaagcgacg attctgatac ggcgtgacgc tcagcccttt gcttttcgcc aacgcaaaca
    16381 gctccttcgc ctgggcaagc gtcggggtga acggtttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgtttcgcg tactcgaaat ggctgtccgc gtgggtgcag acaacaacca
    16501 gcttaacatc gggatcgttt agtacttcgt cgagatcgct gatgaaatgg atatgggaat
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgcg cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgcag cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttgcggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcccc ggcaattaag gtctataaaa aatacggctt tgaaattgaa gggactggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcata ttatatggcg cgggtgaagt
    17521 aagatagtgc cctttttctg agatggaaaa agggtgtcat tcaaaatcga cataccttcc
    17581 tttaaggtat ttatttaccc aatacatata ttgagattta actcataatt aattcctaaa
    17641 gtgtaatatt ttatttttta atatatacgc ctacaatttc ctggagtaaa taaataacaa
    17701 ttaacaagca taatattgcc attgataaaa tagcatgcca taaaaggact tttcagggat
    17761 gagtaatatt gtttacctga cagtaacggg agaacaacaa ggaagcatct ccgcaggttg
    17821 tgggacttct gagtctacag gtaatcgttg gcagagcggg catgaggatg aaatatttac
    17881 attctcactc ttaaataata ttaataatac ggggcttggt tcacagttcc atggtataac
    17941 attttgtaaa ttaattgata aaagcactcc attatttatt aattccatta acaataatga
    18001 acaattattt atgggatttg acttctatcg aataaataga tttggtagat tggaaaagta
    18061 ttattatata caactaagag gcgctttttt atcggctatt catcaccaga tcattgaaaa
    18121 ccaactggat acagaaacaa taactattag ttatgaattt atcctctgtc aacatcttat
    18181 cgcaaatacc gagttcagct atttggcact ccctgaaaat tataaccgat tgtttttacc
    18241 aaattcaaaa aaccaaacaa ataatcgttt caaaacgtta aacagcaaag ctattggcag
    18301 gctacttgct gctggtggcg tatacaatgg gaacattgaa ggattcagag atactgcgga
    18361 aaaactgggt ggagatgcaa taaaaggcta tgatcaaata ctaaatgaaa aaacagcggg
    18421 catagcgata gcaacagcat ctattctttt aacaaagcgt tctaatgttg atacatatac
    18481 agaaataaat agttacttag gcaaacttag aggtcaacaa aaacttcttg atggtataga
    18541 cataatagaa ataatataca ttaagagacc ttcaaaagac ttagctaact tacgaaagga
    18601 gtttaataaa actgtaagaa aaaattttct tatcaaactt tcaaaaacct ccgaagcatc
    18661 tggaagattc aacgccgaag accttttaag aatgagaaag ggcaatgttc ctctaaatta
    18721 taatgttcac cataaactat ctctagatga tggtggtact aatgatttcg aaaatttagt
    18781 attaatcgaa aacgaaccat atcataaagt ttttactaac atgcaatcac gaatagctaa
    18841 gggaatatta gtaggtgaaa gcaaaatcac tccctgggcc attccatctg gctcaattta
    18901 tcctccc
//
LOCUS       DAJTYD010000068        18862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTYD010000068 DAJTYD010000000
VERSION     DAJTYD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.068"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..721
                     /gene="rihB"
                     /locus_tag="OO585_002100"
     CDS             <1..721
                     /gene="rihB"
                     /locus_tag="OO585_002100"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6629691.1"
                     /translation="QIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMASDGD
                     ITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEAARV
                     VFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENYGLA
                     GGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKVGIT
                     IDTDWFWGLVEECVRGYIKTH"
     gene            821..2070
                     /locus_tag="OO585_002101"
                     /pseudo
     CDS             821..2070
                     /locus_tag="OO585_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(2126..3214)
                     /locus_tag="OO585_002102"
     CDS             complement(2126..3214)
                     /locus_tag="OO585_002102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6629692.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3217..4074)
                     /gene="nfo"
                     /locus_tag="OO585_002103"
     CDS             complement(3217..4074)
                     /gene="nfo"
                     /locus_tag="OO585_002103"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6629693.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4148..5197)
                     /locus_tag="OO585_002104"
     CDS             complement(4148..5197)
                     /locus_tag="OO585_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6629694.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIIVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5297..6178
                     /locus_tag="OO585_002105"
     CDS             5297..6178
                     /locus_tag="OO585_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6629695.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6383..7852
                     /gene="lysP"
                     /locus_tag="OO585_002106"
     CDS             6383..7852
                     /gene="lysP"
                     /locus_tag="OO585_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6629696.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8145..10125
                     /gene="cirA"
                     /locus_tag="OO585_002107"
                     /pseudo
     CDS             8145..10125
                     /gene="cirA"
                     /locus_tag="OO585_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10157..10993)
                     /gene="yeiG"
                     /locus_tag="OO585_002108"
     CDS             complement(10157..10993)
                     /gene="yeiG"
                     /locus_tag="OO585_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6629697.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11251..11919
                     /gene="folE"
                     /locus_tag="OO585_002109"
     CDS             11251..11919
                     /gene="folE"
                     /locus_tag="OO585_002109"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6629698.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11936..13093
                     /locus_tag="OO585_002110"
     CDS             11936..13093
                     /locus_tag="OO585_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6629699.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13235..14275
                     /gene="galS"
                     /locus_tag="OO585_002111"
     CDS             13235..14275
                     /gene="galS"
                     /locus_tag="OO585_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6629700.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14555..15553
                     /gene="mglB"
                     /locus_tag="OO585_002112"
     CDS             14555..15553
                     /gene="mglB"
                     /locus_tag="OO585_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6629701.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15614..17134
                     /gene="mglA"
                     /locus_tag="OO585_002113"
     CDS             15614..17134
                     /gene="mglA"
                     /locus_tag="OO585_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6629702.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17150..18160
                     /gene="mglC"
                     /locus_tag="OO585_002114"
     CDS             17150..18160
                     /gene="mglC"
                     /locus_tag="OO585_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6629703.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18403..>18862)
                     /locus_tag="OO585_002115"
     CDS             complement(18403..>18862)
                     /locus_tag="OO585_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6629704.1"
                     /translation="GGIETWEDAAEFLLLGAATLQVTTGIMQYGNRIVEDMASGLSHY
                     LADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGCGRCYISCYDGGHQA
                     MEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIELGEVKFKKGEKEHPVTL"
BASE COUNT         4501 a         4646 c         5014 g         4701 t
ORIGIN      
        1 acaaatcgtt gccgataata ttcacggtga aaccggactg gatggtccgg tattcgagtc
       61 gctgacccgc caggcagaaa gtacccacgc ggtgaaatac atcattgata ccctgatggc
      121 aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg cggtggcaat
      181 gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt gtgctaatgg gcggcgctta
      241 cggtacaggc aacttcacgc catctgccga gttcaacatc tttgctgatc cggaagcagc
      301 ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc tcaccaacca
      361 gactgtttgc accccggacg tgattgcacg gatggaaagg gcaggcggcc ctgccggaga
      421 gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaaaact acggcctggc
      481 tggcggcccg gtgcacgacg ccacctgcat cggttatctg attaaccctg atggcattaa
      541 aacccaggag atgtacgtcg aagtagacgt caacagtggc ccttgctatg ggcgtaccgt
      601 ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca tcactattga
      661 taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca aaacccatta
      721 agtgttgata acaagccggg tcagtatcgc aagatccaag cccggctatt tctgatgggc
      781 agaaagccgt cgcccaatat gacatgaaga gataataact atggatgtca tgagaagtgt
      841 tctgggaatg gtggtattgc tgacgattgc gtttttgctg tcagtaaaca agaagaagat
      901 cagcctgcgt accgttggcg cggcgttagt gttacaggtc gtgattggcg ggattatgct
      961 ttggttaccg gcagggcgtt gggtcgctga aaaagtcgct tttggcgtgc ataaagtgat
     1021 ggcgtacagc gacgcgggta gcgcatttat cttcggttcg ctggtcgggc cgaaaatgga
     1081 cacgctgttt gatggcgcag gatttatctt tggtttcagg gtattaccgg caattatctt
     1141 cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggattt taattcgcat
     1201 tctcggcggt atattccaga aagcattaaa tatcagcaag attgagtcat tcgtcgcggt
     1261 caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct ttatcgatca
     1321 tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga ttgctggttc
     1381 gacaatgatt ggttacgccg ccctgggcgt acctgtggaa tatttgctgg cggcatcgtt
     1441 aatggcgatc ccaggcggga tcttgtttgc tcgcctgtta agcccggcta cggaatcttc
     1501 gcaggtttcc tttaataacc tctctttcac cgaaacaccc caaaaagcat tattgaagcc
     1561 gctgcgacag gggcaatgac cgggctgaaa atcgccgccg gtgtagcgac agttgttatg
     1621 gcatttgtcg ccatcattgc gttaattaat ggtattatcg gcggcgttgg cggctggttt
     1681 ggttttgcac atgcctcgct ggagtccatt ttaggttacc tgttggcccc attggcgtgg
     1741 gtgatggggg ttgactggag tgatgcaaat cttgccggga gtttgattgg gcagaagctg
     1801 gcgatcaatg aatttgtcgc ttatctcaat ttctcgccat atctgcaaac gggtggcact
     1861 ctggatgcta aaaccgtggc gattatttct ttcgcgttgt gcggtttcgc taactttggt
     1921 tctatcgggg tggtggtggg ggcgttttct gcggttgcgc cacaccgtgc gccggaaatc
     1981 gcccaacttg gtttacgcgc gctggcggcg gcgacacttt ctaacctgat gagtgctgct
     2041 attgcaggat tctttattgg tttagcgtag tgcgcaattt atgagccgga taagatgcgg
     2101 tcaagcgtcg aatccggcaa cattgttaca ccattggcac taatgaaagc gcgttatcgg
     2161 cagacagggt agggttgttt aagctgccgc tggcgcgcga aattgccgcg caggctaccg
     2221 caaaacgggc gctgtcgcgg aaactgtatc cttcaagaaa gctatatacc aggcccgcca
     2281 taaaaccatc gccagcacca aaactgtcta ctgtcgtatg cgctggcgcg gtcagcaaaa
     2341 attgttctcc atccttttcg ctgcaataaa ctgactcatc gggcaaataa acaaacagtt
     2401 gctgaacacc ttgctgatgc aatgcattca ctgcggcatt acggtcagca tcgctggtga
     2461 tcgcctgtcc ccataaaatt tccagctccg gtaaagtggg tttcagggtg tgaatatgcg
     2521 ccagccagtg tttgattttg cccgctttga attctgaaac ggtatcgaca aacaccggga
     2581 tttcatcagc gagggtaaag acccattcca gcgcctcggc tgtcaggttg caatcagcca
     2641 gtaccacgcc cgcatgacga agtaaatcgc gcgacccgtt cagtagttgc ggtgtcaact
     2701 gttccagcag atgggtatcg ttaatcgcca gcactgtttg atcgtctcga ttggcaattg
     2761 ccagatacgt cgatgtgctt tgaccatgca aacgaacgca gccggagaca ttcacgccgg
     2821 cacggcgcgt ttcttccagg agcatttcgc cataaaagtc atcgccaatc actgaaagca
     2881 aatggacgtc acggcctaac agcgccagat tgtgggcgat gttgcgtccc acgccgcccg
     2941 ccgagcaatg aattgtaccg ggatgagaag ccgcttgcgg gtaacggata tccgcgatcc
     3001 cgcgaatatc catattgatt gtccccacca ctacgcagta ttcctgctcg gtgagaatgt
     3061 aacctttgcc tttaatccgc cctttgcgca ttaaatccat aatatgcgcc gcaacgcgcg
     3121 aacgactgat ttgcagcatg tccgcaattt cgttctgctg aatcagcggg ttacgccgta
     3181 aaattgcaag gatctccttt tcccggttat tcatcttcag gctaccgctt tttcagtttg
     3241 ttgcgctttc agccaggcga tctcttctgc ccagatatcc gggttgatgg tttcaaggat
     3301 cagcggaatg ccgtcgaaac ggtcgtcctg catgatccag cggaacgcat catgaccgat
     3361 attgccttca ccgaggctat gatggcggtc aacgcggctg ccaaaggtgc ttttcgcatc
     3421 gttaaggtgc atcccgcgca gatacttaaa gccaacaata cgggcaaaat ccgcgaacgt
     3481 tttctcgcat tcggctggag tacgcaaatc atacccggca gcgaaagcat ggcaggtatc
     3541 aatgcagacg ccgacacggg atttatcttc cacgccgtcg ataatcgccg cgagatgttc
     3601 gaatttaaac cctaagttac tgccctgacc ggcggtgttt tcaatcaccg ctgttacacc
     3661 ttgagtttta tccagcgcaa tgttgatgga ttcggcaata cgcgcaaggc aatcctcttc
     3721 tgaaatctgc atcagatggc tgccagggtg gaagttgagc aaagaaagcc ccagttgttc
     3781 gcaacgctgc atttcatcta taaaggcatc gcgcgatttt tccagagctt cagtgaccgg
     3841 atgtccgagg ttaatcagat agctgtcgtg gggaagaatt tgcgccgatg tgtagtgata
     3901 tttttcacag gctgctttga attcatcgat ggtttgcgtc gtgagcggtg cggcacgcca
     3961 ctgacgttgg tttttggtga acaaggcaaa cgcggttgcg tcgatttcgg cggcgcgaat
     4021 tgcggcattt gccagaccgc cagcagcact aacgtgcgct ccaatgtatt tcatgcgagg
     4081 actcctgtta aacccgctgg aggaaaacgg taatgatagc gggttaacag gagcaagatg
     4141 tagtggttta tgcgatgacg ctttgaatca catagttaat cgcaccacca ccaacaatca
     4201 gccaggcaaa cagtaccagt gccatcagca gaggtttcgc cccggctttt ttcagcgcgc
     4261 tgacgtgagt ggtcagaccc agcgccgcca tcgccattgc cagcaggaag gtatccagcg
     4321 ttaccagcat gttcaccacg ctctgcggta acaggtggaa cgagttaaag atggcaacga
     4381 tgatgaacaa gatggcaaac cacggaatag tgattttgct tttctcgccg ctgttcgccc
     4441 cagacagctg tttaacacgc gccgccagca ggatgaggaa cggagccagc atcatcacgc
     4501 gcagcatttt ggaaataact gctgcgtttt ccgcatccgg gctgatggca tgacctgccg
     4561 ccaccacctg cgccacttcg tgcacagtag aaccaatgta gataccgaaa gtttccggac
     4621 taaaccattg agacatcagc ggatatatcg ccgggtagag gaaaatcgcg acggtcccga
     4681 agataacaac ggttgcaaca gccacggtta ctttactggc ttccgctttc actaccggct
     4741 cagtcgccag taccgcggca gcaccacaga tactgctacc ggcaccgatc aaccagctgg
     4801 tgtgcttatc cagaccaaac actttctgcc ccaggaagca agccagcagg aaggtactgg
     4861 acagcgtcaa cacgtcaatg atgatcccac tgataccgac atcggcaatt tgcgagaacg
     4921 tcagacggaa gccataaaga atgataccca gacgtaataa atattgcttg gcaaacagca
     4981 caccaccgtc acagcttttc cagatgtgcg gatagatggt gttgcctaaa accatcccca
     5041 acaagattgc gagggtgagg gcactaaacc cggcacccgc aaccgcggga atggaaccac
     5101 cccacagggc gaccccggtg ataactgcac tcagggctaa ccccggaata aaatgccaca
     5161 gtgtacgatg ttgtttctgt aaggtgatat tcgtcataac cctctcctta taaccgggca
     5221 taaggttacg actaactggt ttaaaaataa aattgattat atatttataa ttaatcttta
     5281 taagtggtaa gcgactatgc acatcaccct ccggcagttg gaagtttttg cagaagtatt
     5341 gaaaagtgga tcaaccaccc aggcgtcggt gatgctggcg ttgtcgcaat cagcagtgag
     5401 cgcagccttg accgacctgg aagggcagct tggcgtgcaa ctgtttgatc gcgtggggaa
     5461 aagactggtt gttaatgaac acgggcggct gctctatccg cgtgcgttgg cattgcttga
     5521 acaggcggtt gaaatcgaac aactgtttcg cgaagacaac ggcgcgattc gtatctatgc
     5581 cagtagtacc atcggtaact acattctgcc tgcagttatc gcccgttatc gccatgatta
     5641 tccgcagttg ccgattgaac ttagcgttgg gaacagccag gacgtgatgc aagcggtgct
     5701 ggatttccgc gttgatattg gctttattga aggaccgtgc cacagcactg aaatcatttc
     5761 tgaaccgtgg ctggaagacg agctggtggt tttcgccgcg ccgacttcgc cgttggctcg
     5821 tggtccggtc accttagaac agctggccgc tgcgccgtgg atcctgcgtg aacgcggttc
     5881 cggcacgcgg gagattgtcg attatctatt gctgtcacat ttaccgaagt ttgagatggc
     5941 gatggaatta ggtaactccg aggcaatcaa acatgcggtg cgtcatgggt tgggaattag
     6001 ttgcctgtcg cgacgggtga ttgaagatca attgcaggca ggcacattaa gtgaagttgc
     6061 ggtccctctg ccgcgcctga tgcgtacgtt gtggcgtata catcatcggc aaaaacacct
     6121 ttccaacgcg ctacggcgct ttctggacta ttgcgatccc gcgaatatac cgcgttaagt
     6181 tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga cgcagcgccc tcagcatgca
     6241 ttcgccagaa aagagattgg ctgctttact tataatccca gggcgatcat gaaggtgtct
     6301 tataaccgtg tatttctgcc ggaaggattg ccaatcgtct gctacaatcg cgcctcattt
     6361 ttaagatgga tagcattttt gtatggtttc cgaaactaaa accacagaag cgccgggctt
     6421 acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc attggcggtt ccatcggtac
     6481 aggtcttttt gttgcctctg gcgcaacgat ttctcaggca ggtccgggcg gggcattgct
     6541 ctcgtatatg ctgattggcc tgatggttta cttcctgatg accagtctcg gtgaactggc
     6601 tgcatatatg ccggtttccg gttcgtttgc cacttacggt cagaactatg ttgaagaagg
     6661 ctttggcttc gcgctgggct ggaactactg gtacaactgg gcggtgacta tcgccgttga
     6721 cctggttgca gctcagctgg tcatgagctg gtggttcccg gatacaccgg gctggatctg
     6781 gagtgcgttg ttcctcggcg ttatcttcct gctgaactac atctcagttc gtggctttgg
     6841 tgaagcggaa tactggttct cactgatcaa agtcacgaca gttattgtct ttatcatcgt
     6901 tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag cctgcgggct ggagcaactg
     6961 gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg atgatcggcg tagctatgat
     7021 tgtcggcttc tctttccagg gaaccgagct gatcggtatt gctgcaggcg agtccgaaga
     7081 tccggcgaaa aacattccac gcgcggtacg tcaggtgttc tggcgaatcc tgttgttcta
     7141 tgtgttcgcg atcctgatta tcagcctgat tattccgtac accgatccga gcctgctgcg
     7201 taacgatgtt aaagacatca gcgttagtcc gttcaccctg gtgttccagc acgcgggtct
     7261 gctctctgcg gcggcggtga tgaacgcagt tattctgacg gcggtgctgt cagccggtaa
     7321 ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg gcgtgtgacg gtaaagcgcc
     7381 gcgcattttc gctaaactgt cgcgtggtgg cgtgccgcgt aatgcgctgt atgcgacgac
     7441 ggtgattgcc ggtctgtgct tcctgacctc catgtttggc aaccagacgg tatacctgtg
     7501 gctgctgaac acctccggga tgacgggttt tatcgcctgg ctggggattg ccattagcca
     7561 ctatcgcttc cgtcgcggtt acgtattgca gggacacgac attaacgatc tgccgtaccg
     7621 ttcaggtttc ttcccactgg ggccgatctt cgcattcatt ctgtgtctga ttatcacttt
     7681 gggccagaac tacgaagcgt tcctgaaaga taccattgac tggggcggcg tagcggcaac
     7741 gtatattggt atcccgctgt tcctgattat ttggttcggc tacaagctga ttaaaggaac
     7801 tcacttcgta cgctacagcg aaatgaagtt cccgcagaac gataagaaat aagtttcctc
     7861 ccttccttgc taagccctct caaccgagag ggctttttca attccatttc cctgacaaat
     7921 catgcggata taaaatttaa catttggatt gataattgtt atcgtttgca ttatcgttac
     7981 gccgcaatca aaaaaggctg acaaatcaga ggctgttccg gctttctggg atgatcacct
     8041 gcatacaaat aagtccaccg cgatgctgcc gtacgcaagg ggacgtgaag aaggtgtgag
     8101 cgataaccca ttttattttc gtagttacct cattgagata tggaatgttt aggttgaacc
     8161 ctttcgtacg ggtcaggctg tgtttgtccg ctatttcttg tgcatggcct gtgttagcgg
     8221 tcgatgatga tggcgaaacg atggttgtca ctgcatcttc cgttgaacaa aacctcaaag
     8281 atgcacccgc cagtatcagc gtcattaccc aggaagacct gcagcgaaaa ccggtacaga
     8341 atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa ggggataacc
     8401 gtaagggcgt tagtattcgt ggtctggaca gcagctacac cctgattctc gtcgacggta
     8461 aacgcgttaa ctcccgcaat gccgtcttcc gccacaatga tttcgatctg aactggatcc
     8521 cggtcgattc catcgaacgt attgaagtgg ttcgtggccc gatgtcgtcg ctatacggct
     8581 ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag aaatggtcgg
     8641 gtaccgttac cgtcgatacc accattcagg aacatcgcga tcgcggtgat acctataacg
     8701 gtcagttctt taccagtgga ccattaattg atggtgtgct gggaatgaaa gcttacggca
     8761 gcctggcaaa acgtgaaaag gatgacccgc aaaactcaac gaccaccgat accggagaaa
     8821 tgccgcgtat tgaaggattc tccagccgcg acggcaatgt cgaatttgcc tggacaccga
     8881 atcaaaatca cgattttact gccggatacg gtttcgaccg tcaggaactc gctggacaaa
     8941 aaccgcctgg aacgccagaa ctactccgtc agccataatg ggcgttggga ttacggcacc
     9001 agcgaactga aatactacgg tgagaaagtc gagaacaaaa accctggcaa cagcagcccg
     9061 ataacttccg aaagcaatac ggtcgacggc aaatacacgt tgccgctgac ggcgattaat
     9121 cagtttctca cggttggcgg tgaatggcgt cacgacaaac ttagcgatgc ggtgaacctg
     9181 accgggggaa ccagctccaa aacgtctgcc agccagtacg cgctgtttgt ggaagatgaa
     9241 tggcggatct tcgagccgct ggcgctgacg accggcgtgc gtatggacga tcacgaaacc
     9301 tacggtgaac actggagtcc gcgtgcctac ctggtttata acgccaccga caccgtaacg
     9361 gtgaaagggg gctgggcgac ggcatttaaa gcaccttctc tgttgcaact tagccctgac
     9421 tggacgagca attcctgccg tggcgcatgt aagattgtgg gtagcccgga tctgaaacca
     9481 gaaaccagcg aaagttggga gctggggctt tactacatgg gtgaagaagg ctggctggaa
     9541 gggggggaat ccagcgttac cgttttccgt aacgatgtga aagatcgtat cagcatcagc
     9601 cgtacgtctg acgtcaacgc tgcaccgggc taccaaaact ttgttggttt tgagacgggc
     9661 gctaacggac ggcgtatacc ggtatttagc tactacaacg ttaacaaagc tcgtattcag
     9721 ggcgtggaaa ccgaactgaa aattccgttc aacgatgaat ggaaactgtc gatcaactac
     9781 acctacaacg atggtcgtga tgtcagcaac ggcgaaaaca aaccgctatc cgatctgccg
     9841 ttccatactg ctaacggtac gctggactgg aaaccgctgg cgctggaaga ctggtcattc
     9901 tatgtttctg gtcactatac cgggcagaaa cgcgccgaca gcgcgacggc taaaacaccg
     9961 ggcggttata ccatctggaa taccggcgcg gcctggcagg tgactaaaga cgtcaaactg
    10021 cgcgcaggcg tgctgaacct tggcgacaag gatctcagtc gtgacgacta cagctataac
    10081 gaagacggac gtcgttactt tatggcagtg gattatcgct tctgatgaga agatgcccgg
    10141 cgaaccgggc ggactttcac ttcagtaaat actgcgcatg gaagcgcagg tgatcctcta
    10201 taaaagaggc gatgaagtag taactgtgat catatcccgg ctgaatacgc agcgtcatcg
    10261 gccacgcttt ctggcgcgcg gcttctgcca gtaccgcagg ttgcaactgg tcggcaagaa
    10321 actgatcatt atcgccctga tcgataagcg tcgggatcgc atcctgcgcg ttactggcat
    10381 acatcagcgc gcaactgtcc cattccagcc atgcattttt gtcctcacct aaatagctgc
    10441 taaacgcttt gattccccac gggacgctgc acggattcac aattggcgca aaggccgaaa
    10501 cgctggtgta tttacccgga tttttcagcg ccataatcag cgcaccgtga ccacccattg
    10561 agtgaccgct aatggcgcag cggtcgctga cattaaattg cgactgaacc agcgccggta
    10621 attcatcgcg cagataatca tacatccggt aatgcgtcgc ccacggcggt tgcgtggcat
    10681 taagataaaa gcctgcgccc tggcctaaat cgtagccatc gtcgttggca accttttcgc
    10741 cgcgcgggct ggtgtctggc atcaccagta caatccccag ttctgccgcc acgcgctggg
    10801 cacccgcctt ggtggtgaag ttctcgtcat tgcaggttaa tccggaaagc cagtacagca
    10861 ctggtggcgg ggtgtgatca cgagggggtg ggagaaagat actgaacgtc atcgggcagt
    10921 ttaaggtact ggagtcgtgt cgccagcgtt gctgccagcc ttcaaaacag cggtgctctt
    10981 cgagcatttc catgcgtggc tccttgttgt gttgtttgca atttcttcat cataccgttt
    11041 attcaatgtg ctgtgagtaa ctttcacttc cgtatttgca taacgatgtt ttaacatctg
    11101 ctgatgaaag gcagcggcaa ttacaataat tatcgctgtg aatactggat tatgtgcgcc
    11161 gcctcacgca caataatcag gctgtaaatc agcttaataa ctttgccccc acgcagggcg
    11221 gaggcgtcac acctgcagga gaaatcataa atgccatcac tcagtaaaga agcggccctg
    11281 gttcatgaag cgttagttgc gcgaggactg gaaacaccgc tgcgcccgcc cgtgcatgaa
    11341 atggataacg aaacgcgcaa aagccttatt gctggtcata tgaccgaaat catgcagctg
    11401 ctgaatctcg acctggctga tgacagtttg atggaaacgc cgcatcgcat cgctaaaatg
    11461 tatgtcgatg aaattttctc cggtctggat tacgccaact tcccgaaaat caccctcatt
    11521 gaaaacaaaa tgaaggtcga tgaaatggtc accgtgcgcg atatcactct gaccagtacc
    11581 tgtgaacacc attttgttac catcgatggc aaagcgacgg tggcttatat cccgaaagat
    11641 tcggtgatcg gtctgtcaaa aattaaccgt atcgtgcagt tctttgccca gcgtccgcag
    11701 gtgcaggaac gtctgacgca gcaaattctt attgcgctac aaacgctgct gggcaccaat
    11761 aacgtggctg tctccattga tgcggtgcat tactgcgtga aagcgcgtgg catccgcgat
    11821 gcaaccagtg ccacaacaac gacctctctt ggtggattgt tcaaatccag tcagaatacg
    11881 cgccacgagt ttctgcgcgc tgtgcgtcat cacaactgat taaaaggcag gaaccatgga
    11941 gcgcaacgta acgctcgatt ttgttcgcgg cgtcgccatt ctggggatcc tgctattaaa
    12001 catcagcgcc tttgggctac caaaggcggc ttatctcaat cccgcctggt acggcgctat
    12061 tacgccgcag gatgcatgga cctgggcatt tctcgatctc atcggccagg tgaaattcct
    12121 cacgcttttt gcgctgctgt ttggtgcggg cctgcaaatg ttgctgcccc gtggcagacg
    12181 ctggatccag tcgcggttaa cgctgttagt cttgctgggt tttattcacg gtttattgtt
    12241 ctgggacggc gatattctgc tggcttacgg gctggtgggc ttaatctgct ggcggctggt
    12301 gcgcgatgcg ccatcggtaa aaagcctgtt taatacaggc gtcatgcttt atctggtggg
    12361 gcttggcgtt ttgctgttat tggggctgat ttccgacagc cagaccagcc gcgcctggac
    12421 gccggatgca tcggctattt tgtatgaaaa atactggaag cttcacggcg gcgttgaagc
    12481 gatcagtaat cgtgccgatg gtgttggcaa cagtttactg gcactgggcg cacagtatgg
    12541 ctggcaactg gcagggatga tgctcattgg tgcggcgctg atgcgcagcg gctggctgaa
    12601 agggcagttc agcttacgtc actatcgtcg tactggtttt gtgctggtgg cgattggggt
    12661 gaccattaac cttcctgcca tcgccctgca atggcagctg gactgggcgt atcgctggtg
    12721 tgccttctta cttcaaatgc cgcgggaact gagtgcgccg tttcaggcga ttggctatgc
    12781 gtcgctgttt tatggcttct ggccgcaatt gagccgcttt aagctggtgc ttgcgatcgc
    12841 ctgcgtcgga cggatggcgc tgaccaacta tctattgcaa acgctgattt gtaccatgct
    12901 tttttaccac ctcggtttgt ttatgcattt tgaccgcctg gagctgctgg cgtttgttat
    12961 tccggtatgg ctggcgaata tcctcttctc tgttatctgg ctgcgttact tccgccaggg
    13021 gccggtggaa tggctctggc gtcagttaac tttgcgtgct gccggaacgg caatatctaa
    13081 aacatcaaga taacgatctg gatcacattc gttaacaaaa cggctgtaac cgtttccatt
    13141 gctgtgactc gattcacgaa gtcctgtatt cagtgctgac aaaatagccg ccagcaagca
    13201 gtcatttact gcaatctcat aacaggtagt gaatatgatc accattcgtg atgtagcgcg
    13261 tcaggctggc gtctctgtgg caacggtttc ccgggtgctc aataacagca cgctggtcag
    13321 tgccgacacg cgtgaagcag taatgaaagc cgtgagtgag ctggattatc ggccaaacgc
    13381 caatgcccag gcgctggcaa ctcaggttag cgacaccatt ggcgtggtgg tgatggacgt
    13441 ttctgatgcg tttttcggcg cgctggtaaa agcggtggat ctagtcgctc agcagcatca
    13501 gaaatacgtg ctaatcggca atagctatca tgaagcggaa aaagagcgtc acgccattga
    13561 ggtgttaatt cgccagcgtt gtaatgcgtt gattgttcac tcaaaagcat tgagtgacga
    13621 tgaactggcg caatttatgg ataacattcc cggtatggtg ttaatcaacc gcgttgtgcc
    13681 ggggtacgcc catcgttgcg tttgcctgga taatctcagc ggtgcccgaa tggcgacgcg
    13741 catgttgctg aataacggtc atcaacgtat tggttatctt tcttccagtc acggcattga
    13801 agatgacgcc atgcgtaaag caggctggat gagtgcgttg aaagagcagg atattattcc
    13861 gccggaaagc tggattggca ctggtacgcc ggacatgccg ggcggtgagg cggcgatggt
    13921 tgaactgctg gggcgcaatc tacaacttac cgctatattt gcttataacg acaatatggc
    13981 cgctggcgca ctgacagcat taaaagataa tggcattgcg attccgttac atctctcaat
    14041 catcggtttc gatgatattc ccatcgcccg ttacaccgac ccgcaattaa cgaccgtgcg
    14101 ttatcccatt gcttcaatgg ctaaattagc caccgaactg gccttgcagg gggcagcagg
    14161 caatattgat cctcgtgcca gccactgttt tatgccgacg ttagtgcgtc gccattctgt
    14221 cgcaacgcgc cagaatgcgg cggcgatcac taactcaaca aatcaggcga tgtaaccgct
    14281 ttcaatctgt gagtgatttc acagaatctt aacaatgtga tagctatgat tgcaccgttt
    14341 taacgttgta acccgtatgt aacagtgaat aatcactttc accgaggtaa tagcgtcata
    14401 acaacaatta aagccgtttt ctggagcgtt accgggcatg gaagaacgaa ttttaaaaag
    14461 tgagcttcgg cgttcagtaa cacttcatta actctactgc cctgccgagc atttatctca
    14521 agcactaccc tgcataagaa aaaccggaga taccatgaat aagaaggtgt taaccctgtc
    14581 tgctgtgatg gccagcatgt tattcggtgc cgctgcacac gctgctgata ctcgcattgg
    14641 tgtaacaatc tataagtacg acgataactt tatgtctgta gtgcgcaagg ctattgagca
    14701 agatgcgaaa gccgcgccag atgttcagct gctgatgaat gattctcaga atgaccagtc
    14761 caagcagaac gatcagatcg acgtattgct ggcgaaaggg gtgaaggcac tggcaatcaa
    14821 cctggttgac ccggcagctg cgggtacggt gattgagaaa gcgcgtgggc aaaacgtgcc
    14881 ggtggttttc ttcaacaaag aaccgtctcg taaggcgctg gatagctacg acaaagccta
    14941 ctacgttggc actgactcca aagagtccgg cattattcag ggcgatttga ttgctaaaca
    15001 ctgggcggcg aatcagggtt gggatctgaa caaagacggt cagattcagt ccgtactgct
    15061 gaaaggtgaa ccgggccatc cggatgcaga agcacgtacc acttacgtga ttaaagaatt
    15121 gaacgataaa ggcatcaaaa ctgaacagtt acagttagat accgcaatgt gggacaccgc
    15181 tcaggcgaaa gataagatgg acgcctggct gtctggcccg aacgccaaca aaatcgaagt
    15241 ggttatcgcc aacaacgatg cgatggcaat gggcgcggta gaagcactga aagcacacaa
    15301 caagtccagc attccggtgt ttggcgtcga tgcgctgcca gaagcgctgg cgctggtgaa
    15361 atccggtgca ctggcgggca cagtactgaa cgatgctaac aaccaggcga aagcgacctt
    15421 tgatctggcg aaaaacctgg ccgatggcaa aggtgcggct gatggcacca actggaaaat
    15481 cgacaacaaa gtggtccgcg taccttatgt tggcgtagat aaagacaacc tggctgaatt
    15541 cagcaagaaa taagactgat tgggcgcagt cgtttactgc gcccttattg tatggccaac
    15601 aaggtaaaat tatatggtca gctcaacgac tccgtcttcc ggggaatact tgttggaaat
    15661 gagcggtatc aacaagtcct ttcctggtgt taaggcactt gataacgtta atttaaaagt
    15721 ccggccacat tctatccatg cattaatggg ggaaaacggt gcaggaaaat cgacattatt
    15781 aaaatgcctg tttggtattt atcaaaaaga ctccggcacc attttattcc agggtaaaga
    15841 gatcgatttc cattccgcca aagaggcact ggaaaatggt atttcgatgg tacaccagga
    15901 gttaaacctg gtattacaac gttcggtgat ggataacatg tggctggggc gatatcccac
    15961 caaaggcatg tttgtcgatc aggacaaaat gtaccgcgaa accaaagcga tttttgatga
    16021 actggatatt gatatcgatc cgcgtgcgcg cgtcggcaca ttatccgttt cgcaaatgca
    16081 gatgatcgaa atcgccaaag cgttttccta taacgcgaaa attgtgatta tggatgaacc
    16141 gacttcttcg ttaaccgaaa aagaggtcaa tcatctgttc actattattc gtaaattaaa
    16201 agagcgcggc tgcggtattg tttatatctc gcataaaatg gaagaaatct tccagttatg
    16261 tgatgaagtt accgtattgc gcgacggtca gtggatcgcc accgaaccgc tggcaggact
    16321 gacgatggac aagatcatcg ccatgatggt tgggcgttct cttaatcagc gttttcctga
    16381 caaagaaaac aagccgggcg aagtcatcct cgaggtgcgt aacctaacgt cactgcgcca
    16441 gccgtcgatt cgcgatgtct cgtttgatct gcataaaggg gagatcctcg gtattgccgg
    16501 gctggtgggg gcgaaacgta ccgatattgt tgagacgtta tttggtattc gcgagaaatc
    16561 ggctggcacc attacgttgc acggcaaaca gatcaataac cataatgcca acgaagccat
    16621 aaaccacgga tttgcactgg taactgagga gcgccgctca acgggaattt atgcctatct
    16681 ggatattggt tttaactcgt taatttccaa tattcgcaac tacaaaaata aagttggttt
    16741 actggataaa tcgcggatga aaagcgatac ccagtgggtg attgattcga tgcgggtaaa
    16801 aacaccgggg catcggacgc aaattggttc gctctcaggt ggtaatcagc aaaaggtgat
    16861 tattgggcgc tggctactaa cgcaaccaga aatattaatg ctcgatgaac cgacgcgcgg
    16921 tattgatgtc ggggcgaagt ttgagatcta tcagttaatt gccgaactgg cgaagaaagg
    16981 caaagggatt attattatct cctctgaaat gcctgagttg ttagggataa cagaccgtat
    17041 tctggtcatg agcaatggtc tcgtttccgg aattgtcgac acaaaaacaa caacgcaaaa
    17101 cgaaattctg cgtcttgcgt ctttgcacct ttaagattag gggcttccca tgagtgcgtt
    17161 aaataagaaa agttttctta cttacctgaa agagggcggt atttacgtcg ttcttttagt
    17221 tttgctggcg attattattt tccaggaccc aacattttta agtctgttga acttaagtaa
    17281 tattctcacc cagtcatcgg tgcgtattat tatcgcgctc ggtgtggcag ggttaattgt
    17341 cacccagggg accgaccttt ctgcggggcg tcaagtaggg ctggcagcag tggtggcagc
    17401 gacattattg cagtccatgg ataacgccaa taaagtgttc ccggaaatgg cgacgatgcc
    17461 gattgcgctg gttattctga ttgtctgtgc cattggtgcg gtgatcggtt tgatcaacgg
    17521 tctgattatc gcttatctca acgttacgcc gttcattacc acgctcggca cgatgatcat
    17581 cgtctatggc atcaactcgc tctattacga ctttgtcggg gcgtcgccaa tttctggttt
    17641 tgacagtggc ttctctacct ttgctcaggg ctttgtcgcg ctggggagtt tccgtctctc
    17701 ttacatcacc ttctacgcgt tgattgcggt ggcgttcgtc tgggtgttgt ggaacaaaac
    17761 ccgcttcggt aagaacattt ttgccattgg cggtaacccg gaagcggcaa aagtatctgg
    17821 tgtcaacgtc ggcctgaacc tgctgatgat ctacgcgttg tctggcgtgt tctatgcctt
    17881 tggcgggatg ttagaagccg gacgtatcgg ctctgccacc aacaacctcg gctttatgta
    17941 tgagctggat gctatcgcgg cgtgcgtggt aggcggtgta tcgttcagcg gcggtgtggg
    18001 gacggtgatt ggcgtggtga ccggggtaat tatttttacc gtcatcaact atggcctgac
    18061 gtatatcggc gtaaacccat gctggcagta catcatcaaa ggggcgatta ttatcttcgc
    18121 cgtagcgctg gattcactga aatacgcgcg taagaaatga tgtgaatgtg gtccgcattc
    18181 ggtcataaac gcacataaca tcatgatctg tgtggttttg taggccggat aaggcgttca
    18241 cgccgcatcc ggcaacagga gccaaatgcc ggatgcaacg ctggcgcgtt ttatccggcc
    18301 tacaataagc tcaacatact gatttgtatg gttttgtagg ccggataagg cgttcaagcc
    18361 gcatccggcg acaggaacca aatgccggat gtgacgcggg ttctacaacg ttaccgggtg
    18421 ttctttctcg cctttcttaa acttcacttc cccgagctca atacaaccca ccgggcagac
    18481 gtgaccacaa agcagacaac ccacacattt ctcggtatta caatgcggtg tgcgggtttt
    18541 ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac agcgtccaca
    18601 gccaacacat ttgtcaagat tgatacgggg atagacaata taactgcggt ctaaatcttc
    18661 cgccgggaca atattgttat tcgccagacc taccatctcc tgcaacgaat caaatccctg
    18721 atcggcgaga taatgcgata acccgctcgc catatcttct acgatgcgat tgccgtactg
    18781 cataatgccg gtggtgacct gtaacgttgc tgcgccgagc aataaaaact ccgccgcatc
    18841 ctcccaggtt tcaatgccgc cg
//
LOCUS       DAJTYD010000069        18732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTYD010000069 DAJTYD010000000
VERSION     DAJTYD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.069"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..883
                     /locus_tag="OO585_002116"
     CDS             <1..883
                     /locus_tag="OO585_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6629705.1"
                     /translation="IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGV
                     LGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGM
                     MLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVTDWL
                     VKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCRG
                     LIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGA
                     LAYIFLVRQPINDPRKD"
     gene            complement(932..2587)
                     /gene="tsr"
                     /locus_tag="OO585_002117"
     CDS             complement(932..2587)
                     /gene="tsr"
                     /locus_tag="OO585_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6629706.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2706..3152)
                     /gene="hpaR"
                     /locus_tag="OO585_002118"
     CDS             complement(2706..3152)
                     /gene="hpaR"
                     /locus_tag="OO585_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6629707.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3424..4713
                     /locus_tag="OO585_002119"
     CDS             3424..4713
                     /locus_tag="OO585_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6629708.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4710..6176
                     /gene="hpaE"
                     /locus_tag="OO585_002120"
     CDS             4710..6176
                     /gene="hpaE"
                     /locus_tag="OO585_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6629709.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6178..7029
                     /gene="hpaD"
                     /locus_tag="OO585_002121"
     CDS             6178..7029
                     /gene="hpaD"
                     /locus_tag="OO585_002121"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6629710.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7039..7419
                     /locus_tag="OO585_002122"
     CDS             7039..7419
                     /locus_tag="OO585_002122"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6629711.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7530..8333
                     /gene="hpaH"
                     /locus_tag="OO585_002123"
     CDS             7530..8333
                     /gene="hpaH"
                     /locus_tag="OO585_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6629712.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8344..9132
                     /gene="hpaI"
                     /locus_tag="OO585_002124"
     CDS             8344..9132
                     /gene="hpaI"
                     /locus_tag="OO585_002124"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6629713.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9307..10683
                     /gene="hpaX"
                     /locus_tag="OO585_002125"
     CDS             9307..10683
                     /gene="hpaX"
                     /locus_tag="OO585_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6629714.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10693..11579
                     /gene="hpaA"
                     /locus_tag="OO585_002126"
                     /pseudo
     CDS             10693..11579
                     /gene="hpaA"
                     /locus_tag="OO585_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11830..13392
                     /gene="hpaB"
                     /locus_tag="OO585_002127"
     CDS             11830..13392
                     /gene="hpaB"
                     /locus_tag="OO585_002127"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6629715.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13410..13922
                     /locus_tag="OO585_002128"
     CDS             13410..13922
                     /locus_tag="OO585_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6629716.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14315..16465
                     /gene="btsT"
                     /locus_tag="OO585_002129"
     CDS             14315..16465
                     /gene="btsT"
                     /locus_tag="OO585_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6629717.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16596..16799
                     /locus_tag="OO585_002130"
     CDS             16596..16799
                     /locus_tag="OO585_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6629718.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16810..17766
                     /gene="yjiA"
                     /locus_tag="OO585_002131"
     CDS             16810..17766
                     /gene="yjiA"
                     /locus_tag="OO585_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6629719.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17868..>18732)
                     /locus_tag="OO585_002132"
     CDS             complement(17868..>18732)
                     /locus_tag="OO585_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6629720.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKES"
BASE COUNT         4285 a         5183 c         4991 g         4273 t
ORIGIN      
        1 catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc
       61 cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg ggatgtttat
      121 taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg
      181 cgagcacgta gaactgactg ccgttgagca agcttatctc aatgcaggta gcgtcaatgc
      241 ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gtacaatgtg
      301 gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcctggct
      361 tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatgacggc
      421 tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ccgactggct
      481 ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt
      541 ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga cggcggttct
      601 gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgcc ggggcttgat
      661 ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag
      721 cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata ccacccactc
      781 attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac tggcgtacat
      841 cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt atgcccgata
      901 acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc tcctcgctat
      961 ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca gccgatgttt
     1021 cacgctgctg ttgctgaatc cggaacactg ccactgcttc ggtcaggcga ctggcctgct
     1081 cttccagcgc ggcggcggca gcggcagact cttccaccag cgcggcgttc tgttgagtta
     1141 cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc tgctcatcag
     1201 aagcagaagc aatttcgccc ataatgtccg tcacgcgggt cacggcgctg acaatctccg
     1261 ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc cccaccgagt
     1321 cttcaatcag gcttttaatt tcacgagccg cctgggcgct gcgctgggcc agattacgca
     1381 cttctcccgc gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca gcctcaaccg
     1441 ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg ctgataatat
     1501 cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg ttatctacca
     1561 ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg gcctggcgcg
     1621 cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct gccgtctctt
     1681 ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg gtggcgattt
     1741 cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg gtacgcatca
     1801 gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta gagccatcca
     1861 cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca atcaggcgat
     1921 tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg accgccagta
     1981 cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca ttgttatcgc
     2041 tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac tgcttctcga
     2101 aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg cctgcaccta
     2161 acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt ttgatctctg
     2221 ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc gcccagtttt
     2281 tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg gttgaaccgc
     2341 taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg gtgttacgcg
     2401 tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga atggtttgta
     2461 aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg cctgatgtca
     2521 gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc ttgatacgtt
     2581 ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga aagcaaaaac
     2641 tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta tcagatacta
     2701 aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag agcaataaat
     2761 tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc ttcaatctgc
     2821 cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc ctctttggtt
     2881 agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac taaaccgtcg
     2941 cgttccatcc gcgtgagaat cccggtcagg cttgggcgca aaatgcaggc gcgatacgcc
     3001 agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg ccactgttgc
     3061 tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc ttctcgcgcc
     3121 tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa ttcaaaatca
     3181 ttaatataga aacagttaat cgtcacgtta ccgggactgc gctgcccctg caattcctga
     3241 ttttatcaaa attgatgata ttttattaac ttactgatat ttaatatttt attatcttta
     3301 aatgtaaata gtttgttaat tagatcacat ttacatcact tacttttgcc aaactacgca
     3361 atcgggaata aaagcaatca ttaacatatt aatgattaag ataatcgaat ccgaggagtg
     3421 gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc gcagccaact tgatgcatgg
     3481 caggaagcgt tccagcaatc cccctacaaa gccccgccta aaactgcggt ctggtttatt
     3541 aaaccgcgca atacggtgat tggttgcggt gaaccgattc cctttccaca gggtgaaaag
     3601 gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa cggcgacgaa agtacgtgaa
     3661 gaagatgcgg cagagtacat cgccggatat gcgctggcta acgacgtcag cctgccggaa
     3721 gagagctttt accgcccggc aatcaaagca aaatgtcgtg atggattctg ccccattggc
     3781 gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct ataccgagat caacgggcgt
     3841 cctgccgatc actggaatac cgccgattta caacgtaacg ccgcgcagtt gctgagcgcc
     3901 ctgagcgaat ttgccacgct gaatccaggc gacgccattc tgctcggcac gccacaggcg
     3961 cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg cagaaggttt cccgccgctg
     4021 gaaaatccgg tagtggacga acgtgaagtg accacgcgca agagcttccc aacgcagcca
     4081 cacccgcacg gcacgctgtt tgccctcggc ctgaactacg ccgaccacgc cagcgaactg
     4141 gaatttaagc caccggaaga accgctggta ttcctgaaag cgccaaatac cctcactggc
     4201 gataaccaga cctccgtgcg tccaaacaat attgaataca tgcactatga agcggagctg
     4261 gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag ccgatgccat ggattatgtc
     4321 gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa ctactaccgc
     4381 cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac catcgtgccg
     4441 aaagaggcga tcccggaccc gcataatctg acccttcgca ccttcgtcaa cggcgagtta
     4501 cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat cgcctactta
     4561 agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc caaaggctta
     4621 tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg ccgcctggtg
     4681 aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg atcaacggca
     4741 aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt gaagtgctgg
     4801 cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca gcgaaagagg
     4861 cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg cgccgtctgg
     4921 gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg gacaccggcc
     4981 tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac tttgaatttt
     5041 tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac aagatgctca
     5101 actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg aacgtaccgt
     5161 ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc gcggtactga
     5221 aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg ctggaagccg
     5281 gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca ggggatgcgc
     5341 tggttcgtga tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc accgggcgca
     5401 acatcatgaa aaacgccggg ctgaaaaaat actccatgga actgggcggt aaatcgccgg
     5461 tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg ttcaccatct
     5521 tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag caaagcatct
     5581 acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg ggcgatccga
     5641 acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa aaagtctccg
     5701 gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc ccggataaac
     5761 cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg gtgctggcag
     5821 acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc gcctgcctgc
     5881 tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag tacggcctcg
     5941 cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt ggcattgaag
     6001 ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca tttggcggcg
     6061 taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg ttcgcggaaa
     6121 tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga gtctgatatg
     6181 ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct ctctgaactg
     6241 ccagggaaaa accacggttg ccgccagggc gcgatcgacg ggcataaaga gatcagcaag
     6301 cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg gctggtcaac
     6361 agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag caacgagctg
     6421 ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt ggggcagctt
     6481 attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat tcccagcctg
     6541 aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa gcacttcaaa
     6601 gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg caagctgggc
     6661 gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct tgccagcggt
     6721 tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa cagctacacc
     6781 cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga aggccagttc
     6841 aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga aggcaatatg
     6901 cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg caaggtggag
     6961 tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt tttcccgctt
     7021 cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa catccgcgaa
     7081 gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc cacgggtatt
     7141 tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca gatggccgac
     7201 gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg tcgcagcctg
     7261 gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca cttcgccgcc
     7321 ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca tccgacgctg
     7381 aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg cccggtggcg
     7441 ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat aaggcgcagc
     7501 cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac accctgatcg
     7561 cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc tcgctggatt
     7621 acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt cgcctgaaaa
     7681 tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa gcaatgcagg
     7741 ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg ttcttccacg
     7801 atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg gagctggctt
     7861 tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc tacaacgcca
     7921 cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac atcgatccgg
     7981 aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc aacgccgggg
     8041 tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg atctccgccc
     8101 tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg ctgaatcatc
     8161 cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta caactggaag
     8221 ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt aagggcgaca
     8281 ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt taaggagaga
     8341 acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat cggattatgg
     8401 ctggggctga gtagcagcta cagcgcagag ttactggccg gagcaggatt cgactggttg
     8461 ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca gctacaggcg
     8521 attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc ggtgcaaatc
     8581 aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca aaacgctgac
     8641 gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg cggtgtgggc
     8701 agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca aaaagccaac
     8761 gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa cttaccgcag
     8821 attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct gagcgccgat
     8881 atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga gcaggcgatc
     8941 gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga gcaactggca
     9001 aaacgctatc tggaactggg cgcgctattt gtcgccgtcg gcgttgacac caccctgctc
     9061 gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc cgtgaagccc
     9121 ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac caaaccgtag
     9181 gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc ggaccggata
     9241 aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc cattagagga
     9301 agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc gaatcagcat
     9361 aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg cctgatcgtc
     9421 tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg ctttgccgga
     9481 ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc taccaccctg
     9541 ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag tattgtcggt
     9601 gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac tgccaccatg
     9661 tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat taccgaagcc
     9721 ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta tttccgcgcc
     9781 cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg ctcgatcgtt
     9841 tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca gtggctgttt
     9901 ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg gcttgatgac
     9961 tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca agagatgatg
    10021 gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca cgccatgcaa
    10081 caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac cctggcgtat
    10141 ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat cctgcaaagt
    10201 tttaatcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc gcagatttgt
    10261 accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga acgaaggcat
    10321 cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc ggcaactgat
    10381 cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt cagcgcaatg
    10441 gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc gatcggtatt
    10501 gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat gatcggctgg
    10561 ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc gctgctggtg
    10621 attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg agcgaccccg
    10681 taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa agagtacgat
    10741 gaaagcctgg gcacggacga tgtgcattat cagtcgttcg cccgcatggc ggcatttttt
    10801 ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt cctcaatagc
    10861 ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc cctgtttgtc
    10921 ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga cggtcatgtg
    10981 ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc gggcactcgg
    11041 gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa ctggcggcgc
    11101 tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct ggacgggaac
    11161 acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac gcaaaactcg
    11221 acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt tttaatatgc
    11281 tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac gaactgcata
    11341 tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg ccaaaacggt
    11401 tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct gataacgccg
    11461 tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg cgctttttta
    11521 atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct gtgacgtgaa
    11581 aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc tccgcctgga
    11641 gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca aaaccgtacc
    11701 cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc ccctgcaagc
    11761 tttaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat taatactgta
    11821 gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt caccggggaa
    11881 gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga gcgagtgaaa
    11941 gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca actgtacgac
    12001 gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac cggcagcggc
    12061 ggctataccc ataaattctt ccgcgtggcg aaaagtgcca acgacctgcg ccagcaacgc
    12121 gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac cccagactac
    12181 aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca gttcgagcag
    12241 aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa ccacgcgatt
    12301 gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt ttacatcaag
    12361 ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt tgccaccaac
    12421 tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat gggcgaaaac
    12481 ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa attaatctct
    12541 cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta cccgctctcc
    12601 agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat cccatgggaa
    12661 aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga aggcggtttt
    12721 gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga cttcattaca
    12781 gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtacaggcc
    12841 gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga ctcgatgtgt
    12901 tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc actgcaaacc
    12961 tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga acgcaacgtt
    13021 accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc gcagatcgac
    13081 cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca gcacatcaag
    13141 atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca cgaactgtat
    13201 gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg ccaggcacaa
    13261 agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac
    13321 cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat gctggataag
    13381 ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc ctgcgctttc
    13441 gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag ggcgacgccg
    13501 gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca ccatcgctga
    13561 tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac ggtaagttgt
    13621 gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg ggcatgacag
    13681 gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg ctggcgcagc
    13741 cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg caggcaattg
    13801 gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca gaaggtcacg
    13861 gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa gctgcgattt
    13921 aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc tcaatcacat
    13981 atccacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc
    14041 ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg gcaacatcac
    14101 tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac ggcttcggcc
    14161 tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag tgagtgaatc
    14221 cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg cacctgtaag
    14281 ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac tattcaagca
    14341 cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg
    14401 tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt
    14461 ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg accccacgcg
    14521 cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca accgttacgt
    14581 gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg gtccggtgct
    14641 cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg tagtactggc
    14701 cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg gcgcatctct
    14761 cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc tgtttggctg
    14821 tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta aagccctggc
    14881 cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg
    14941 tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta ttggtatcgt
    15001 gctgctggtt gcctctatct atttcggtgg cgtgattgct cacgatccgt actggggtcc
    15061 ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg cgtttgtttc
    15121 cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa
    15181 aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg aactgaaaat
    15241 gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg ctctgttccc
    15301 gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc
    15361 cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg gctacggcgc
    15421 aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca tcatcgaacc
    15481 gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca tgcctaacct
    15541 gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag acgttaccgc
    15601 acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc agatcctgca
    15661 aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg cgccgacgct
    15721 ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca tgggcttctg
    15781 gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg atgcgggtac
    15841 tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa
    15901 aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta
    15961 cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt
    16021 cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg ttgtgctgat
    16081 taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat ggctgcttat
    16141 ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga tggaaggctt
    16201 cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc tgacggcgca
    16261 gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag gcctgagtat
    16321 tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct ggcttgcggt
    16381 gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc cggaaggcgg
    16441 cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta tccggcctac
    16501 aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat ccctagcccg
    16561 gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta ggacaggcaa
    16621 aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac gacaactacg
    16681 tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc
    16741 gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat
    16801 ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa
    16861 accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa
    16921 aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc cacgcagatc
    16981 aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga agacgcacta
    17041 ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct ggtcattgaa
    17101 tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca tgagatttta
    17161 tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca tgccgatgag
    17221 cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg tattctgctg
    17281 acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac
    17341 gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct tctgttcaac
    17401 accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt ccactttatc
    17461 gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc
    17521 agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt
    17581 tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag
    17641 cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca tagcacgatg
    17701 gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc gggactcaaa
    17761 aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat attcctccag
    17821 aatatgaggg cattcgggct taattatctc tgaacgtgca acacttccta cgactcctta
    17881 tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt ttcgtacatt
    17941 ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa cccactctga
    18001 ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg tgagaaaaca
    18061 atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt ttcttccgct
    18121 gcggcctgcg gtacatcaaa gaaattattt gcccggaacg ttaaggccat tgcaggtgtt
    18181 ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt atcttccagc
    18241 gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc ctgtgggatt
    18301 ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact tatattcagc
    18361 gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca cttatcaaat
    18421 ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca aacgttcagg
    18481 atggcagagc ctggctgctg ccaggtttta ccatcatcat taagcaaatt gatgccatcc
    18541 caacgtgtta atgtatccac caactgacgg cgcggatcgc tctgtgtcaa accagatgtc
    18601 gctgcttgca gagtaggtaa aaaaagcctc aggttaagat cctgacgact ggtttggcga
    18661 ataacatccc atgcctgatc agcagttaag cgtggctttt gctcaagcag tcggtcgatc
    18721 tccgtaacgc ga
//
LOCUS       DAJTYD010000070        18036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTYD010000070 DAJTYD010000000
VERSION     DAJTYD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.070"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..301)
                     /locus_tag="OO585_002133"
     CDS             complement(<1..301)
                     /locus_tag="OO585_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6629721.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSM"
     gene            768..2000
                     /gene="mdtM"
                     /locus_tag="OO585_002134"
     CDS             768..2000
                     /gene="mdtM"
                     /locus_tag="OO585_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCR6629722.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2041..3321
                     /locus_tag="OO585_002135"
     CDS             2041..3321
                     /locus_tag="OO585_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCR6629723.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            3437..4589
                     /locus_tag="OO585_002136"
                     /pseudo
     CDS             3437..4589
                     /locus_tag="OO585_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            4599..5367
                     /locus_tag="OO585_002137"
                     /pseudo
     CDS             4599..5367
                     /locus_tag="OO585_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5364..5621
                     /locus_tag="OO585_002138"
     CDS             5364..5621
                     /locus_tag="OO585_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCR6629724.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5575..6546
                     /locus_tag="OO585_002139"
     CDS             5575..6546
                     /locus_tag="OO585_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR6629725.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            6614..7786
                     /locus_tag="OO585_002140"
     CDS             6614..7786
                     /locus_tag="OO585_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6629726.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(7799..8353)
                     /gene="kptA"
                     /locus_tag="OO585_002141"
     CDS             complement(7799..8353)
                     /gene="kptA"
                     /locus_tag="OO585_002141"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6629727.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            8603..9286
                     /locus_tag="OO585_002142"
     CDS             8603..9286
                     /locus_tag="OO585_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629728.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9283..9744
                     /locus_tag="OO585_002143"
     CDS             9283..9744
                     /locus_tag="OO585_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6629729.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            9757..10929
                     /gene="iadA"
                     /locus_tag="OO585_002144"
     CDS             9757..10929
                     /gene="iadA"
                     /locus_tag="OO585_002144"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6629730.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            10994..11905
                     /gene="hypT"
                     /locus_tag="OO585_002145"
                     /gene_synonym="qseD"
     CDS             10994..11905
                     /gene="hypT"
                     /locus_tag="OO585_002145"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6629731.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(11898..12290)
                     /gene="iraD"
                     /locus_tag="OO585_002146"
     CDS             complement(11898..12290)
                     /gene="iraD"
                     /locus_tag="OO585_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6629732.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12287..12370)
                     /gene="idlP"
                     /locus_tag="OO585_002147"
     CDS             complement(12287..12370)
                     /gene="idlP"
                     /locus_tag="OO585_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6629733.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            12962..13792
                     /locus_tag="OO585_002148"
     CDS             12962..13792
                     /locus_tag="OO585_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6629734.1"
                     /translation="MSIPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(13933..14706)
                     /gene="uxuR"
                     /locus_tag="OO585_002149"
     CDS             complement(13933..14706)
                     /gene="uxuR"
                     /locus_tag="OO585_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6629735.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(14921..16381)
                     /locus_tag="OO585_002150"
     CDS             complement(14921..16381)
                     /locus_tag="OO585_002150"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6629736.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(16462..17646)
                     /gene="uxuA"
                     /locus_tag="OO585_002151"
     CDS             complement(16462..17646)
                     /gene="uxuA"
                     /locus_tag="OO585_002151"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6629737.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
BASE COUNT         4202 a         4569 c         4546 g         4719 t
ORIGIN      
        1 ccatggaata tcgcatcagg cgaaatgcca tatgaatatc ctcgcggctc tgatgttcaa
       61 tcactacgta aatataacca tcaccttcac gggtctttac cgaccacaga atatcggagt
      121 gtagcgcccg caatttttca tcgacaaagc tggcggattc cagttttaag ctgtcgagat
      181 cgcacagttc acgtaaatct ttgggtaagt ggatctccat aaaatcccgc gcggtgtcag
      241 ggtgagtgag aaaggattta aataatgcgt catgtggcgt gctggtcgtg aagtttgtca
      301 tggcgatccg tcacctgtag ataagatggc ccgactttag cgactaacaa aaccattctc
      361 acccggcaat ttctcgttcc ccgaggtgct ttccaggcgt ctgcaaacga tacatttttc
      421 cggaaacagg ctcgcgtttt acatttcctg tacccggcag atattcccct ttccaccgcg
      481 ctcttttgat taaccgcaaa ttttaattac acttaaggtg tatattttct atgcaaccca
      541 tcaattaaag aggtgtaatg tgctgatgac tatttgtaat cgttatacat tctgacctgg
      601 agtcagaagg tatttctctg tctgtgtgtt cacaggcagt gtggttgatt acatgaaatt
      661 cagtacattt gcagtctcgt tgcccttctc gcctgccttt cgtcattacc gacggtattg
      721 aatttcgttt tccccgttgg ggttctccga acaaggagtt gtttgttatg ccacgttttt
      781 ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt gattttgtat gactttgccg
      841 cgtacctgtc gacggatctg atccagccgg ggatcattaa tgtggtccgt gattttaatg
      901 ccgatgtcag tctggccccg gcagccgtca gtctctatct cgcgggcggt atggcgttac
      961 agtggctgct ggggccgctt tccgacagga ttggccgcag gccggtgctg attaccgggg
     1021 cgctaatatt tacccttgcc tgcgctgcga caatgttcac aacgtctatg acacagtttc
     1081 ttatcgcgcg tgcaattcag ggcaccagta tctgttttat tgccaccgtt ggttatgtca
     1141 cggtgcagga ggcgttcggc cagacaaaag ggatcaagtt gatggcgatt atcacctcca
     1201 tcgtactgat tgcgccgatt attggcccgc tttccggcgc agctctgatg cactttgtgc
     1261 actggaaagt cctttttgcc atcattgcag ttatgggttt tatctcattt gttggcttac
     1321 tgctggcgat gccagagacg gtgaagcgcg gcgcggttcc atttagcgcc aaaagcgtct
     1381 ttcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct ctttggcgca gcaaccatct
     1441 ctttaagcta tatcccaatg atgagttggg tggctgtctc gccggtgatc ctgatcgacg
     1501 cgggcagctt aacaacttcg cagttcgcct ggacacaggt tccggtgttc ggcgcggtga
     1561 ttgtcgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc gaccgaaccg cgctttatct
     1621 ggcgtgctgt acccattcaa ctggtcggcc tcgcgctgtt gattatcggc aatctgctgt
     1681 caccgcacgt ctggctgtgg tcggtgctgg gcaccagttt gtatgctttc gggattggtt
     1741 tgatcttccc gaccttattc cgctttacgc ttttttccaa taacttaccg aaagggaccg
     1801 tctccgcatc gctgaatatg gtgatcctga tggtgatgtc tgtctcggtc gaaatcggcc
     1861 gctggctgtg gtttaacggc ggtcgcttgc cgtttcatct gttagccgtc gtggcgggcg
     1921 ttatcgtcgt tttcaccctg gcaggattgc ttaatcgcgt gcgccaacat caggctgccg
     1981 agctggcgga ggagcggtga tttttgcgcg atcctgccgt caggctctat tcttaacgtt
     2041 atgaataaac tcattgaact cagacgcgcc aaaatgttgg cgctctcttt actgcttatc
     2101 gccgctgcta cctttgtcgt tacgctgttt ttgccgccca atttttgggt gagcggcgtg
     2161 aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg actggtttgc ggtggtggcg
     2221 ctgtttcgcc gcgtgccgat tccgatcatt tctcgtcata cggcgattat cccgcgtaat
     2281 aaagaccgga ttggcgaaaa tctcggtcag ttcgtgcagg aaaaatttct cgatacccag
     2341 tcgctggtgg cattgattcg acgtcacgaa ccggcgttgt tgattggcaa ctggtttagt
     2401 cagccggaga acgcccgccg cgttggtcag catctgttgc agatcatgag cggttttctt
     2461 gaactgaccg atgatgcgcg tattcagcgc ctgcttaagc gcgcggtcca tcgggcgatt
     2521 gataaggtcg atctttccgg aaccagtgcg ttgatgctgg agagtatgac caaaaacgat
     2581 cgtcatcagg tgctgctcga tacgctgatc gcacagttga tcgcccttct ccagcgcgat
     2641 aaatcgcgca agtttattgc ccagcaaatt gttcgctggc tggagagcga gcatccgctg
     2701 aaagccaaaa ttttgcctac tgaatggctg ggcgaacata gcgcggagtt ggtttctgac
     2761 gcggtgaatt ctttgcttga tgatatcagc cgcgatcgtg cgcatcagat ccgtcatgcg
     2821 tttgatcgcg ccacttttgc cctgatcgac aagctgaaaa acgatccaga aatggcagcg
     2881 cgagccgatg ccgtaaaaag ttatctgaaa gaagatgaag cttttaaccg ctatctcagt
     2941 gaattgtggg gggatttacg ggaatggctg aaagcggata tcaacagtga agattctcgt
     3001 gtgaaagaac gtatcgcgcg ggcgggtcaa tggtttggcg aaacgttaat tgccgatgat
     3061 gccttgcggg catcgttaaa tggtcacctg gaacaagccg cgcaccgcgt cgcgcctgag
     3121 ttttccgcat tcctgacgcg ccacatcagc gatacagtaa aaagctggga tgctcgggat
     3181 atgtcgcggc aaatcgagtt aaatatcggc aaagatctgc agtttatccg tgtcaacggt
     3241 acgctggttg gcggttgtat tgggctaatt ttatatttgt tgtcgcagct cccggccttg
     3301 ttccccctca gcaattttta gaaatccatg aaaaactaag tggctaaccg cgagagagat
     3361 caaataaacc acactaaaag aatggtaata atgtggcctc ttttcattat ctcccgtggt
     3421 acggggaagg aaaatcatgt cacttatcac cgatctaccc gccattttcg atcagttctc
     3481 tgaagctcgc cagaaaggct ttctcaccgt catggatctc aaggagcgcg gcattccgct
     3541 ggttggcact tactgcacct ttatgccgca agagatcccg atggcagccg gtgcggttgt
     3601 ggtttcgctc tgttctacct ctgatgaaac cattgaagaa gcggagaaag atctgccgcg
     3661 caacctctgc ccgctgatta aaagcagcta cggcttcggc aaaaccgata aatgccccta
     3721 cttctacttt tcggatctgg tggtcggtga aaccacctgc gacggcaaaa agaaaatgta
     3781 tgaatacatg gcggagttta agcccgttca tgtgatgcag ttgccgaaca gcgttaagga
     3841 cgatgcctcg cgtgcgttat ggaaagccga gatgctgcgc ttgcaaaaaa cggtagaaga
     3901 acgttttggg cacgagatta gcgaagatgc tctgcgcgat gccattgcgc tgaaaaaccg
     3961 cgaacgtcgc gcactggcca atttttatca tcttgggcag ttaaatcctc cggcgcttag
     4021 cggcagcgac attctgaaag tggtctacgg cgcaaccttc cggttcgata aagaggcgtt
     4081 gatcaatgaa ctggatgcaa tgaccgcccg cgttcgtcag cagtgggaag aaggccagcg
     4141 actggacccg cgtccacgca ttttaatcac cggctgcccg attggcggcg cagcagaaaa
     4201 agtggtgcgg gcgattgaag agaatggcgg ctgggttgtc ggttatgaaa actgcactgg
     4261 ggcgaaagcg accgagctaa tgcgtggcgg aaacgggcga tgtctacgac gcgctggcgg
     4321 ataaatatct ggcgattggc tgctcctgtg tttcgccgaa cgatcagcgc ctgcaaatgc
     4381 tcagccagat ggtggaagaa tatcaggtcg atggcgtagt tgatgtgatt ttgcaggcgt
     4441 gccataccta cgcggtggaa tcgctggcga ttaaacgtca tgtgcgtcag cagcacaaca
     4501 ttccttatat cgctattgaa acagactact ccacctcaga tgtcgggcag ctcagtaccc
     4561 gtgtcgcggc ctttattgag atgctgtaag gagtggcagt gacatattcg attggcattg
     4621 attctggttc taccgccacc aaagggatct tactggcaga cggcgtgatt acgcgccgtt
     4681 tcctcgttcc aacacccttt cgtccggcaa cagcaattac tgaagcctgg gaaactctgc
     4741 gcgaagggtt agagacaacg ccgtttctga cgctcaccgg ctatggacgg caactggtgg
     4801 attttgccga taaacaagtg acggaaatct cctgtcacgg gctgggcgca cggtttcttg
     4861 cgccagcaac gctgcgcggt aatcgacatc ggtggtcagg acagcaaagt gattcagctt
     4921 gatgatgacg gtaacctgtg cgatttcctg atgaatgaca aatgcgcggc gggcaccggg
     4981 cgtttcctgg aagtgatctc gcgcacgctt ggcaccagcg tcgagcaact cgacagcatt
     5041 accgaaaatg tcacgccgca tgccatcacg agtatgtgca cagtgtttgc cgaatcagaa
     5101 gtgatcagcc tgcgctcagc gggcgtcgcg ccagaagcga ttctcgccgg agtgattaac
     5161 gcgatggcgc ggaggagtgc caatttcatt gctcgtctct cctgtgaagc gccgattctg
     5221 tttactggtg gcgttagtca ttgccagacg tttgcccgga tgctggaatc tcacctggga
     5281 atgccggtaa atacccatcc tgatgcgcaa tttgctggcg caattggcgc ggcggtaatt
     5341 ggtcaacgag tgaggacacg ccgatgaaag agtttttatt tttatttcac tccacagtcg
     5401 gcgtcataca aactcgcaaa gcgttgcagg cagcgggcat gacttttcgc gtcagcgata
     5461 ttccccgtga tttacgcggc ggctgcgggt tatgcatttg gctgacctgt ccccccggcg
     5521 aagaaataca atgggtgatc cctgggctga ctgagtcaat ttattgccag caggatggtg
     5581 tctggcgctg cattgcacat taccgggttt ctccgcgtta aggattcatt aaggtcgcta
     5641 cgtatacttc tcgatatctt attgatttgc cttaaaggga ttaccgtgac aaaaagtatt
     5701 tcgttaagta aacgcatctc cgttattgtg attctttttg ccattgtggc agtgtgtact
     5761 ttcttcgtgc aatcttgcgc ccgtaaaagc aatcatgcag catcatttca gaattaccac
     5821 gccaccattg acggcaaaga aattgcaggc ataacgaata atatctcttc gctgacctgg
     5881 tcggcacaaa gcaatactct ttttagcact atcaacaaac ctgccgccat tgttgaaatg
     5941 accaccaatg gtgattttat ccgcacaatt cctctggatt ttgttaagga tctcgagacg
     6001 atcgaataca ttggcgataa tcagtttgtc attagcgatg agcgcgatta cgccatttat
     6061 gtgatttcac tgaccccaaa ctcggaagta aaaatcctca aaaaaataaa aatcccgctg
     6121 caagactccc caaccaattg cggttttgaa gggttagctt attctcgcca ggatcacact
     6181 ttctggtttt ttaaagagaa aaatccgatt gaggtctaca aagttaacgg gctgttaagt
     6241 agcaatgaat tacacatcag caaagacgag gcactacaac ggcaatttac tttagatgat
     6301 gtgtccggcg cagaatttaa ccagcaaaaa aatacgcttc tggtgctgtc tcatgaatct
     6361 cgcgcactcc aggaagtaac cctggttggc gaggttatcg gtgaaatatc actaaccaaa
     6421 ggtagtcgag gtttgtcgca taacatcaaa caggccgaag ggattgcgat ggatgcctct
     6481 ggtaatattt atatcgttag cgaaccaaat cgtttttatc gctttacccc taaatcatcg
     6541 cattaacagg ctggcatatt tcaccttttt gttattaact tgttaacaac aaaaatgaca
     6601 tacaaggtta aacatgcctt cgtcctcgca tcccgtagaa cgcttttctt tcagcaccgc
     6661 gcttttcggg atgctggttc tgaccctagg tatgggatta ggtcgcttcc tttatacgcc
     6721 tatgttgccc gtcattatgg cggaaggggc gttttcattt agccagctct cgtggattgc
     6781 cagcggcaac tatgcggggt atctggctgg cagtctgcta ttttcgtttg gcgcatttca
     6841 ccagccgtcg cgcctacgcc catttctgtt ggtttctgcc ctggcgagag ggttgttgat
     6901 cctcgcaatg gcatggttgc cgccatttat tctggtgtta ttgattcgct tcctggcggg
     6961 tgtcgccagc gccggtatgc tgatttttgg ttcgacgctg attatgcagc acacgcgcca
     7021 tccttttgtg ctggcagctt tgttttctgg cgttggcatt gcactgggca acgaatatgt
     7081 tctggcaggc ctgcattttg ccttctcctc gcaaacgtta tggcaaggcg ccggggcgct
     7141 ttctggcatg atgctgatcg cacttacgct tttaatgccc tcgaaaaaac acgccatcgc
     7201 accaatgcca ttggcaaaaa cggagcaaca gataatgagc tggtggttac tggctattct
     7261 gtacggcctt gcgggatttg gttatatcat cgtcgcgact tacctgccgc tcatggcgaa
     7321 agatgcaggc tcaccattgt taaccgccca tctctggacg ttagtcggct tatcaattgt
     7381 gccaggctgc tttggctggc tatgggcagc aaaacgttgg ggggctttgc cttgcctgac
     7441 ggcgaatttg ctggtgcagg ctatcagtgt gctgcttact ctcgccagcg actctcctct
     7501 cttgcttatc attagtagta tcggttttgg cggtaccttt atgggcacga catctctggt
     7561 gatgactatc gcccgccaac tgagcgttcc gggaaatctc aatcttttag gctttgtgac
     7621 gctcatttac ggcattggac aaatcctcgg cccggcgctg accagtatgc tcggcaatgg
     7681 aacatcggcg cttgccggcg ccactctctg cggcgcggcg gcgttgttta ttgctgcgct
     7741 tatcagcaca gtacaattat ttaagctgca agtggtcact tcatgaggag tgtttccctt
     7801 accactcaag aaattcgacc gcaaccgttg atgtcagcca gactccgttt tccgcttgcc
     7861 agaaaggaat gcctcgtttc gccatttcct gtgctttgac ggttaagaca accggcgatc
     7921 cgtgtcttgc tcccactttg cgtgccgttg cttcatcagc agagagatgc acgtaatgac
     7981 gatcccctgc aatcagcccc tgttttttta tctcgtcaag gaaacggctt gccgtaccgt
     8041 ggtagagaaa ctgtggcggt gttttttcgg caaaggaaat agcgacctgc gaagtcgaat
     8101 gcccctgaac cgcgcggatg cacctaccat cactggaata actaaaacgc tttttatcgc
     8161 tggttgcgac cacggtatcc agcaatgcac gggttaatcg cttgcccgct ttttgggcac
     8221 aaagtatgag cttatcgata tccgcccatc cttcacggtc cagtacgata ccaatcgcct
     8281 ccggtttgtg tcggaggaca aaacttaaaa acttgctcgt atccgccagc tctttttcgt
     8341 tgtgctttgc catctcgttt tcgtccctgg attttctgtt tttatttacc aatgtcattg
     8401 tcactgggtt tatgaatttt ctgcaactca atcctgcgca gaaccttttc cacacagcgt
     8461 aatgtttccc ctatgcaaaa aacgcatagc cagaaacata cttgcatttt ccccataccc
     8521 acatggctga taaggttttt cagttccagg aattttcctg gctcaagcaa cacacaaaca
     8581 caaaaaaaca atggggattg ttatgacaca acaaggggat gctgtagccg gggagctggc
     8641 gacagaaaaa gtgggcatta aagggtatct ggcttttttt ctcaccataa tctttttttc
     8701 cggtgttttt tccggaacag acagctggtg gcgcgttttt gatttttccg ttctgaacgg
     8761 atcgtttggg caactgcctg gtgcgaacgg agccaccacc tcatttcgcg gcgcgggtgg
     8821 tgcgggggct aaagatggct ttctctttgc gctggagctg gcaccgtcag tcattctctc
     8881 tctggggatt atttcaatta ccgatggtct tggtggatta cgcgccgcgc agcagttaat
     8941 gacacctgtg ctaaaaccgt tgttgggcat tccagggatc tgctcgctgg cgctgatcgc
     9001 taacttgcaa aacactgatg ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga
     9061 aattaccgaa cgcgataaag tgattttcgc cgcatatcaa accagcggta gcgccatcat
     9121 caccaactac ttctcttccg gcgtcgcggt gttcgccttt ctgggcacgt ccgtaatcgt
     9181 acctttagcc gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct
     9241 taacttcgaa gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc
     9301 aaaaacgtca tggatatgtt tatcgatggc gcgcgccgtg gctttactat cgccacgacc
     9361 aacctgctgc caaacgtggt gatggcgttt gtcatcattc aggcgctgaa aattaccggc
     9421 ctgctcgact gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg attacccggt
     9481 gaagcggcaa ccgtcctttt agccgcatta atgagtatgg ggggcgcagt cggtgttgcc
     9541 gccagtctgg caaccgctgg cgcattaacc ggtcacgatg tcaccgtttt actgcccgcg
     9601 atgtatttaa tgggcaaccc ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg
     9661 aatgccaaat attacccgca tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc
     9721 tgggttatgc agcttattgt ttaacaagga gttaccatga ttgattatac cgcagccggt
     9781 tttaccctgc tgcagggagc gcatttgtat gcgccggaag atcggggaat ttgcgatgtc
     9841 ctcgtcgcta acggcaaaat tatcgccgtt gccagcaata tcccttctga cattgtaccg
     9901 aactgcacgg ttgtcgatct cagtgggcag atcctctgcc caggttttat tgatcaacac
     9961 gtccatttga ttggcggtgg cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg
    10021 ctaagtcgcc tgacggaagc gggcgtcacg tcagtggttg gtctgctggg caccgactct
    10081 atctctcgcc acccggaatc cctgctcgcc aagacccgtg cgctcaatga agaaggcatc
    10141 agcgcctgga tgctgaccgg cgcttatcat gtcccttccc gcactattac gggttccgtg
    10201 gaaaaagacg tggcgattat cgatcgtgtg attggcgtga aatgcgccat ctctgatcac
    10261 cgttctgccg caccggacgt ttatcacctg gctaatatgg cggcagaatc ccgcgttggc
    10321 ggtttgctcg gcagtaaacc tggcgtcacc gtgttccaca tgggcgacag taaaaaggcg
    10381 ttacagccta tttatgacct gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg
    10441 acccacgtta accgcaacgt accgttgttt gagcaggcgc tggagttcgc gcgcaaaggc
    10501 ggcaccatcg atatcaccag cagcattgac gaaccggtcg cccctgccga aggtattgcc
    10561 cgcgccgttc aggcgggtat tccgctggca cgcgtcaccc tcagctccga cggcaacggt
    10621 agccagccgt tcttcgatga cgaagggaat ttaacccata tcggtgttgc aggttttgaa
    10681 acgttgctgg aaaccgtgca ggtgctggtc aaagactatg atttcagtat cagcgatgcc
    10741 ctgcgcccgc tcaccagtag cgtagccggt ttccttaacc tgagcgggaa aggcgaaatt
    10801 ctgcctggca atgacgcaga cttactagtc atgacgccag aattgcgcat tgagcaggta
    10861 tatgctcgcg gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa
    10921 acggcttaaa cggttgtcag ccgccctaat ggacgtagaa tgcccgcaag ggcggctgac
    10981 agagtaaaac gtaatggatg actgttgtgc ggttttgcat aatattgaaa ccaaatggct
    11041 ttatgatttt ctgaccctgg aaaaatgccg caatttttcc caggcggcag tcagtcgtaa
    11101 cgtctcgcaa ccggcattca gccgccgcat ccgcgcgctg gaacaggcga ttggcgttga
    11161 attgtttaac cgccaggtga cgccgctgca actctcagaa caagggaaaa tcttccattc
    11221 gcagatccgc catctgttgc aacagttaga aagtaacctt gcagagctgc gtggcggcag
    11281 cgattacgcg caacgtaaaa tcaagatagc cgctgcacac tctctttccc tcggtctgtt
    11341 accgtccatt atcagccaga tgccgccgct ctttacctgg gcgattgaag ctattgatgt
    11401 cgatgaagca gtcgataaac tgcgtgaagg gcaaagtgac tgtatttttt cctttcacga
    11461 cgaagatttg ctggaagcac cgtttgacca cattcgctta tttgaatctc aattgttccc
    11521 cgtttgcgcc agtgacgaat acggagaagc aatttttaac ctcgcgcagc cacactttcc
    11581 gttactgaat tacagccgca actcctacat ggggcgattg attaatcgca ccctgacgcg
    11641 ccacagcgag ttaagtttca gcaccttttt tgtctcttcg atgagcgagc tattaaagca
    11701 ggttgccctc gacggctgtg ggattgcctg gctgccggag tatgccatcc aacaagaaat
    11761 tcgcagcggg caactcgttg tgctgaatcg ggacgaactg gtgatcccga ttcaggccta
    11821 cgcataccgt atgaataccc gcatgaatcc agttgccgaa cgcttctggc gtgaactgcg
    11881 cgagctggag attgtgctta gctgacattc tcaagcgtcg cactgcgcca accgccatta
    11941 tcccagacca gatcaaacgt tagcggttca tcgtcgtggt acagcattat ctcaaggctg
    12001 atcgccagag tgtggcagta cgcttctttc aaattaacct gcaacgccgc aatccgtggc
    12061 tcatgaaata acagtgcaga acgcagctga cggcaatacc agctctggag atgtttgtcg
    12121 ctcgggttat gcacttgcca ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa
    12181 tgaggagaat taaaaagagt gaggagatcg ctgcataccg atttacgcag caatgacgtt
    12241 ttattgcctg aaatttcagg taaaacagcc tgaagtgatt ggcgcatcat tttgcgcact
    12301 cctgacgttt agcaacatat gcggcttgcc ctgaacaccg ggcaccactg tattgatgct
    12361 cattttccat agccattccc ttttgttcat tacatccatg tcagtctgag acaggcctga
    12421 atcctgtcag caaagtataa aaccttactt gcctatgaat atctactgac ttttcaggaa
    12481 aaaagtcatg atttgataaa aatcatcaaa ctcaaaaaaa aaccgctagt atattctgaa
    12541 tattccgaat agttacgtag atgaaaatac ttgtcaccgc cgctacttaa ttccggtgga
    12601 aaattattta tttccgcata atcagccatg tattatttga aacaattagt ttgtaaataa
    12661 tataaattga taacatatca aatacatcaa aaataccatt cataggcatt attaatatag
    12721 acatcaatat agcaacatca aattccaatt agtaataaga tatgcttttt aattgcatga
    12781 aaataatttc aaatgagcgt tttttgatac tttagagtgt ttcctaccct ttaatatcac
    12841 cgtgtagagt tgatatcact acaacagaaa catgcatgtc atttttttac ttctttttta
    12901 ctacagaaaa atttatctat ttttctaaac atcggtgaaa tactcataaa aggtaactat
    12961 tatgtctata ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
    13021 tcatcatcca aaagtcgaca ataagttaac acgtgaatat gaatatacac gcatggatac
    13081 ggaaaatatc tatcttctgc ctcttactcg tggaaataac cataattacg atggaaaaag
    13141 cgttgtcgaa attagaaaac ttaatattgg caaacagtcc tggccattta attatgcgac
    13201 agatacatgc agagaattca atggtattac aacaacagga cgtatgctct atcgaaactt
    13261 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
    13321 tacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
    13381 tgaggactat acagttcatg aaataacatt aggccctggt tacaatgttc ctggttatgc
    13441 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
    13501 tgagcagcca agaattgata tctatattga tcagataata actgtctcag gggtggcaaa
    13561 ttcttccgga ttcgcccttg ccgcattact taatgctaat attggaatgg gaaatgaccc
    13621 aataattggt atagaagcct atcctggtac cgcggagatc catgcaaaaa tgggatataa
    13681 cgttatcccc ggtgatgaag atgcgccact caaacgcatg actctgcaac cgtcatctct
    13741 tccagaactg tttgaattaa aaaatggtga atggaattat attggcaaat aaccactgag
    13801 aagaatgagt actaacattc aggttatata gatatttaaa caataccctg caatttacaa
    13861 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacgttta cccttgcgct
    13921 tatcataatc agtcaggcgt cgactttatc cagcggccat gaatcaaaca gatagccgtc
    13981 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
    14041 ctgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg caaggatctg
    14101 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
    14161 gatccacatt ggattgtttt cccgccactg ccaggactga cggaataact ccaccagcat
    14221 gctgttatgc gttgcttcag caatggcgag atggaactgc atgtcaccgc tttcgctgct
    14281 acccggcgcg ctggaagcca gttcacgctc ttccagttgc aatgcctgac gcattttgac
    14341 gatatcttcg cgggtagcct gcagggcggc aaattccgcg atgttgctct caagaagctg
    14401 tcgcgcctgc aacaattcaa aggggccggc atcattgcag acgttggcat ccggactgtc
    14461 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
    14521 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag
    14581 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtacg gcgtctttat
    14641 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
    14701 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
    14761 cgccttatcc ggcctacaac gggcaatttt acggcccccg ttaatctcaa actataccat
    14821 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
    14881 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
    14941 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
    15001 cattctgcgg cagatcatcg gcaaaaatac cgctcaggcc cagcagcgct ttcacgcggt
    15061 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcgaccacgt
    15121 caatggcatt accctgctca tccacgccct gggtgtaacg catccagcca gccacgccca
    15181 gcgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccggg tccagcagac
    15241 gctgcggtaa cttctggctg ccgtccatcg caatctgcca ggtacggtga cgcagagacg
    15301 ggttgctgaa acgctcgatc agcagcgtcg cataggcctt caggtccgta ccctccggca
    15361 tagacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
    15421 ggttagtcat ggtgtcggca atagtttcat agccgccgag gtaaccgagg tatgccagga
    15481 aagagtggct gccgttcagc atacgcagct tcatcatttc gaacggcaca acgtctgcaa
    15541 cgaactgtgc gcccacttta tcccagtccg ggcgaccatt aacgaagtta tcttcaatca
    15601 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
    15661 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
    15721 ggaaggtgac gttctcttca atccatgccg ccagctgcgg gtcacgtgcc tgagccagcc
    15781 ccagtaccgc gaccttcgcc acatgaccgt tttcacgcac gttatcgcag gacatcaccg
    15841 taaatgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
    15901 cggacttcgg cgcagtcggg ttttctagat cgtgcttgat cagcgggtta ttgagatcca
    15961 gttgaccgct tgccgcatca gcgcagtagc ctttttccgt gaccgttaga gagacaatcg
    16021 ccgtttgcgg acgcgccatc gcgttgagaa taccttcgca gccgtcgatt tccggatgca
    16081 gcgcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcta
    16141 cggtgtacag cagttgctgt tttttcaggt tttcgatcag cacgcggtcg ttgcctggca
    16201 tcaggttaac ttcgcagatg ccccagtcgc tgtcggtgct ttccagcaga tgatgggtat
    16261 acagcgcctg atgtgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
    16321 gacgagaatg atcccatgac ggacgggcaa ccggcagatt gctgtcaaca atagtagtca
    16381 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
    16441 gcgcagccat gccggtggat attaacggct aaagaaagcg cgctggatcg ccagttcgac
    16501 accgcgaact tcggccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
    16561 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
    16621 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
    16681 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
    16741 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
    16801 atcgttgtca gcacgcacgc cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct
    16861 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
    16921 gaggatcggg cgcggcggat cgtccgggtg aaccgccata cgcacgccaa cttcttcagc
    16981 aactggaata atcgctttca ggaagacggc aaagttttcg cgcagcttcg ctttatcgat
    17041 atctttgtac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
    17101 cggcagaccg gcaatgatgt tacgcgtcag acgcgctttg tcttcatcgc tcatagtggc
    17161 gaagcgttca gctgcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
    17221 tttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
    17281 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
    17341 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
    17401 gatccactgc tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gtacgctttc
    17461 tacgacagac cacaccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
    17521 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
    17581 cgcctgacgg acatcagcta aagaaaccag atcgtttggg ccgtaccagc gccaggtctg
    17641 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgc ggaaccctgg
    17701 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
    17761 tggcgtataa gttctacctt ttggtaagat atgtcaaccc aaatcctgaa attggttaac
    17821 caaatcacaa gaatttcact ctcatggttg ttgctgcaaa ttgcagcaaa aggtaatcga
    17881 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
    17941 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
    18001 attctctggg tagtattcgg cattggtctg atgctg
//
LOCUS       DAJTYD010000071        17504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTYD010000071 DAJTYD010000000
VERSION     DAJTYD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.071"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..744
                     /locus_tag="OO585_002152"
     CDS             <1..744
                     /locus_tag="OO585_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6629738.1"
                     /translation="SSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSD
                     WQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLL
                     QDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYI
                     DQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRD
                     IRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO585_002153"
     CDS             complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO585_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6629739.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO585_002154"
     CDS             complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO585_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6629740.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO585_002155"
     CDS             complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO585_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6629741.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO585_002156"
     CDS             complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO585_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6629742.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2581..2856
                     /locus_tag="OO585_002157"
     CDS             2581..2856
                     /locus_tag="OO585_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6629743.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2931..3482)
                     /locus_tag="OO585_002158"
     CDS             complement(2931..3482)
                     /locus_tag="OO585_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6629744.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO585_002159"
     CDS             complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO585_002159"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6629745.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO585_002160"
     CDS             complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO585_002160"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6629746.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO585_002161"
     CDS             complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO585_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6629747.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYQRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO585_002162"
     CDS             complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO585_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6629748.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO585_002163"
     CDS             complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO585_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6629749.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO585_002164"
     CDS             complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO585_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6629750.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8340..9404
                     /gene="ulaG"
                     /locus_tag="OO585_002165"
     CDS             8340..9404
                     /gene="ulaG"
                     /locus_tag="OO585_002165"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6629751.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9512..10267
                     /gene="ulaR"
                     /locus_tag="OO585_002166"
     CDS             9512..10267
                     /gene="ulaR"
                     /locus_tag="OO585_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6629752.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERVGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO585_002167"
     CDS             complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO585_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6629753.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11195..11524
                     /gene="bsmA"
                     /locus_tag="OO585_002168"
     CDS             11195..11524
                     /gene="bsmA"
                     /locus_tag="OO585_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6629754.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11673..11948
                     /gene="yjfN"
                     /locus_tag="OO585_002169"
     CDS             11673..11948
                     /gene="yjfN"
                     /locus_tag="OO585_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6629755.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12065..13690)
                     /locus_tag="OO585_002170"
     CDS             complement(12065..13690)
                     /locus_tag="OO585_002170"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6629756.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13774..14832)
                     /locus_tag="OO585_002171"
     CDS             complement(13774..14832)
                     /locus_tag="OO585_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6629757.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14835..15473)
                     /locus_tag="OO585_002172"
     CDS             complement(14835..15473)
                     /locus_tag="OO585_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6629758.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTISRGGYGRSSSARGHWGG"
     gene            complement(15483..15881)
                     /locus_tag="OO585_002173"
     CDS             complement(15483..15881)
                     /locus_tag="OO585_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6629759.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15899..16558)
                     /locus_tag="OO585_002174"
     CDS             complement(15899..16558)
                     /locus_tag="OO585_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCR6629760.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16609..17307)
                     /locus_tag="OO585_002175"
     CDS             complement(16609..17307)
                     /locus_tag="OO585_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCR6629761.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17380..>17504)
                     /locus_tag="OO585_002176"
     CDS             complement(17380..>17504)
                     /locus_tag="OO585_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR6629762.1"
                     /translation="QKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4466 a         4677 c         4187 g         4174 t
ORIGIN      
        1 tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg
       61 tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta
      121 gatctctctg actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga
      181 gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt
      241 cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat
      301 ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt
      361 ttgggacctg gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc
      421 cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc
      481 gaccaacttc acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg
      541 ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat
      601 tcagcagcaa tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg
      661 agtgatcgta tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt
      721 gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa
      781 tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
      841 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
      901 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
      961 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     1021 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     1081 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     1141 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     1201 taacgtttac ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt
     1261 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
     1321 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
     1381 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
     1441 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
     1501 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
     1561 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
     1621 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt
     1681 cgtgtccgct aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct
     1741 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
     1801 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
     1861 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
     1921 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
     1981 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
     2041 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
     2101 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
     2161 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
     2221 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
     2281 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
     2341 aaatgacgcg ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat
     2401 ctcatctttc gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag
     2461 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa
     2521 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
     2581 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
     2641 gccattgaaa ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg
     2701 atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag
     2761 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
     2821 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
     2881 tgccatagtt tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt
     2941 gcgacttccg gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct
     3001 attatccaga accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat
     3061 tgagtgagcg gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc
     3121 acaataagcc ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca
     3181 atacaatacc ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg
     3241 ataatatgtt ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt
     3301 agaaagatat acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag
     3361 gggacttccg ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt
     3421 aacgcaacgc catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc
     3481 ataaaaacac ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat
     3541 tacatcccgc aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg
     3601 ttaggaccgt gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt
     3661 tgtgggttaa tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg
     3721 ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca
     3781 attcccggcg tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg
     3841 ttcagttcat actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg
     3901 ccaaagaagt agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc
     3961 catgcggtgg catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg
     4021 tagagttcaa gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg
     4081 ccgctcatat cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg
     4141 ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag
     4201 gtcaccagcc catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt
     4261 tgcattatgc cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg
     4321 ctttcaccac ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg
     4381 gcccgcaata gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt
     4441 cgccaaatgg cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga
     4501 tatgcccgat tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc
     4561 cgatatctgg gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct
     4621 tgctgatgga gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc
     4681 ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat
     4741 tggcttcctg atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct
     4801 gggcgaactg gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt
     4861 cgctgcccaa cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg
     4921 tttcaacaat cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca
     4981 ggcgctcgtc agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca
     5041 gtcgcaggcg ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg
     5101 ggatttgttt ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga
     5161 actgacgcgc ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca
     5221 catgaattgg aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg
     5281 tgactttgaa gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg
     5341 ccacgccagc ggcctgcgcg tcacggctgc gctgataaac cacctgctga atacctgcgt
     5401 cgcgccactg ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc
     5461 cgttaaactc ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa
     5521 tcacggtcac ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat
     5581 cggcaatttt ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa
     5641 ccgcacgcac gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt
     5701 cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt
     5761 gcaacatcgg taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg
     5821 agatccagta cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct
     5881 tcaaacaggt tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc
     5941 gccatggtga tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt
     6001 ttcagcgtca ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc
     6061 attgccaggc ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc
     6121 gcctggtagt aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt
     6181 actgcgtcct gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc
     6241 gaatcacgta atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc
     6301 acttccagca gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg
     6361 tatttgttgc cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc
     6421 gcgccgctca actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg
     6481 tttgattggg ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg
     6541 ttgccacaca cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt
     6601 tctgccagtt gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata
     6661 taagccagtg caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc
     6721 tgcatcatcg gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg
     6781 gtgagtttca ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc
     6841 ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag
     6901 aagaacatcg ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg
     6961 ccaaccgcaa tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc
     7021 gcgaagctat agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg
     7081 gagataccgt taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg
     7141 atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact
     7201 ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag
     7261 atggtcatca caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc
     7321 agtttgaggt cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca
     7381 atccatgatg caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc
     7441 tgagtcggct tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc
     7501 gtgcagataa tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc
     7561 cccgcctgct ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga
     7621 cgcagcagca cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag
     7681 ctataggcat cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt
     7741 gcgccgttaa tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg
     7801 aggatgccgg accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta
     7861 ataataacgc tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc
     7921 aacaacgggg cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga
     7981 tctcctgaag aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta
     8041 attttcaaaa gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata
     8101 attccatacg gatagagtgg gatccacaca acgaaacaat tatttattta aatttagtta
     8161 aattcattta gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca
     8221 acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag
     8281 gattaatcaa aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga
     8341 tgagtaaagt gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg
     8401 gtagctggct gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt
     8461 ggtggctagg ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg
     8521 atttctggtg cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc
     8581 agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctacgcacc accccgtttg
     8641 ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg
     8701 atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat
     8761 tcattggacc gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt
     8821 gcatcgtggt caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg
     8881 atgctttcga ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac
     8941 tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc
     9001 tgtatcacag cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc
     9061 agatcgacgt ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga
     9121 ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc
     9181 accacgatat ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga
     9241 tgaaaaaaga tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat
     9301 ttacctggcc actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt
     9361 gcttcactat tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg
     9421 gatagcgtgg agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat
     9481 ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct
     9541 cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg
     9601 aatttcgcct gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa
     9661 aaaggtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa
     9721 tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct
     9781 ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg
     9841 ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct
     9901 gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc
     9961 catcacttta agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac
    10021 cagcggaaaa gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc
    10081 agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat
    10141 tggcgaacgc gtgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg
    10201 caaaaatgct aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg
    10261 tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg
    10321 gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa
    10381 ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca
    10441 cagcagtaga ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag
    10501 cggcgcgata atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg
    10561 aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt
    10621 caccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc
    10681 gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt
    10741 gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc
    10801 gctactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg
    10861 cgccagcgca acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa
    10921 aattacgcac ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat
    10981 atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac
    11041 atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg
    11101 ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca
    11161 ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt
    11221 tatttaccgg tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca
    11281 aggtacgcca cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca
    11341 gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga
    11401 cgcattagca gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt
    11461 catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa
    11521 ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct
    11581 gtgcacaata aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc
    11641 aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc
    11701 tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg
    11761 actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc
    11821 cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc
    11881 gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt
    11941 atgcatgagt gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc
    12001 ggcctacaat tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat
    12061 acgcttacac acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg
    12121 acaaccgtac gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg
    12181 gagaagcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct
    12241 ctcgccccag ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat
    12301 caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc
    12361 ccgcttgctt attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc
    12421 aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac
    12481 caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca
    12541 caaattttgc cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc
    12601 ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca
    12661 tgcgacttaa gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc
    12721 gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca
    12781 gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt
    12841 cctgccccaa caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc
    12901 gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca
    12961 aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg
    13021 catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt
    13081 ccagacgctc tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca
    13141 ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc
    13201 gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca
    13261 atggcgtggc ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat
    13321 gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg
    13381 caagattgtg cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa
    13441 aacgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca
    13501 cattcgccag ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg
    13561 ctagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat
    13621 cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt
    13681 gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg
    13741 gattcctgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa
    13801 acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc
    13861 atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg
    13921 aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt
    13981 attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac
    14041 atagctttcg ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt
    14101 aggatgacca ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact
    14161 tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc
    14221 acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag
    14281 atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc
    14341 ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt
    14401 gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat
    14461 caggcgttcc tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    14521 aaaatccatc cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc
    14581 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc
    14641 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    14701 ctggcgcaga gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat
    14761 atgaaagtcg aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    14821 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    14881 ccgcgcgaaa tggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    14941 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa
    15001 gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc
    15061 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15121 caattttcgt acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa
    15181 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15241 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15301 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    15361 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    15421 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    15481 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    15541 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    15601 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    15661 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    15721 gtaccgctga atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg
    15781 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa
    15841 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    15901 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    15961 actctggctc gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag
    16021 tatcttcggt tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat
    16081 ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16141 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt
    16201 tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga
    16261 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16321 gaaattcatc gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc
    16381 ggctgacagc ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca
    16441 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    16501 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    16561 tttatcctta aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg
    16621 ttggcgttgc agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc
    16681 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc
    16741 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    16801 cgctgtcgaa acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg
    16861 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    16921 cgctgtcgct gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    16981 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17041 caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt
    17101 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17161 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17221 gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17281 cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    17341 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat
    17401 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg
    17461 cacgctggag attcctaccg atgacaacgg catcatcttc tgat
//
LOCUS       DAJTYD010000072        17372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTYD010000072 DAJTYD010000000
VERSION     DAJTYD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.072"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..340
                     /gene="fliH"
                     /locus_tag="OO585_002177"
     CDS             <1..340
                     /gene="fliH"
                     /locus_tag="OO585_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6629763.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            340..1708
                     /gene="fliI"
                     /locus_tag="OO585_002178"
                     /pseudo
     CDS             340..1708
                     /gene="fliI"
                     /locus_tag="OO585_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            1727..2170
                     /gene="fliJ"
                     /locus_tag="OO585_002179"
     CDS             1727..2170
                     /gene="fliJ"
                     /locus_tag="OO585_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6629764.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            2167..3294
                     /gene="fliK"
                     /locus_tag="OO585_002180"
     CDS             2167..3294
                     /gene="fliK"
                     /locus_tag="OO585_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6629765.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            3399..3863
                     /gene="fliL"
                     /locus_tag="OO585_002181"
     CDS             3399..3863
                     /gene="fliL"
                     /locus_tag="OO585_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6629766.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            3868..4872
                     /gene="fliM"
                     /locus_tag="OO585_002182"
     CDS             3868..4872
                     /gene="fliM"
                     /locus_tag="OO585_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6629767.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            4869..5282
                     /gene="fliN"
                     /locus_tag="OO585_002183"
     CDS             4869..5282
                     /gene="fliN"
                     /locus_tag="OO585_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6629768.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            5285..5650
                     /gene="fliO"
                     /locus_tag="OO585_002184"
     CDS             5285..5650
                     /gene="fliO"
                     /locus_tag="OO585_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6629769.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            5650..6386
                     /gene="fliP"
                     /locus_tag="OO585_002185"
                     /pseudo
     CDS             5650..6386
                     /gene="fliP"
                     /locus_tag="OO585_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            6396..6665
                     /gene="fliQ"
                     /locus_tag="OO585_002186"
     CDS             6396..6665
                     /gene="fliQ"
                     /locus_tag="OO585_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6629770.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            6674..7459
                     /gene="fliR"
                     /locus_tag="OO585_002187"
     CDS             6674..7459
                     /gene="fliR"
                     /locus_tag="OO585_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6629771.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            7744..8367
                     /gene="rcsA"
                     /locus_tag="OO585_002188"
     CDS             7744..8367
                     /gene="rcsA"
                     /locus_tag="OO585_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6629772.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(8411..8599)
                     /gene="dsrB"
                     /locus_tag="OO585_002189"
     CDS             complement(8411..8599)
                     /gene="dsrB"
                     /locus_tag="OO585_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6629773.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            8762..8978
                     /gene="yodD"
                     /locus_tag="OO585_002190"
                     /pseudo
     CDS             8762..8978
                     /gene="yodD"
                     /locus_tag="OO585_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9276..10091
                     /locus_tag="OO585_002191"
                     /pseudo
     CDS             9276..10091
                     /locus_tag="OO585_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(10088..11782)
                     /gene="yedQ"
                     /locus_tag="OO585_002192"
                     /pseudo
     CDS             complement(10088..11782)
                     /gene="yedQ"
                     /locus_tag="OO585_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(11953..12135)
                     /locus_tag="OO585_002193"
     CDS             complement(11953..12135)
                     /locus_tag="OO585_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6629774.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(12214..13131)
                     /locus_tag="OO585_002194"
     CDS             complement(12214..13131)
                     /locus_tag="OO585_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6629775.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            13304..14224
                     /gene="yedA"
                     /locus_tag="OO585_002195"
     CDS             13304..14224
                     /gene="yedA"
                     /locus_tag="OO585_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6629776.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(14213..14683)
                     /gene="vsr"
                     /locus_tag="OO585_002196"
     CDS             complement(14213..14683)
                     /gene="vsr"
                     /locus_tag="OO585_002196"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6629777.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(14664..16082)
                     /gene="dcm"
                     /locus_tag="OO585_002197"
     CDS             complement(14664..16082)
                     /gene="dcm"
                     /locus_tag="OO585_002197"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6629778.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(16149..16853)
                     /locus_tag="OO585_002198"
     CDS             complement(16149..16853)
                     /locus_tag="OO585_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6629779.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGE
                     NEGVDHKVIDAFSPAG"
     gene            complement(16893..17258)
                     /locus_tag="OO585_002199"
     CDS             complement(16893..17258)
                     /locus_tag="OO585_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6629780.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         4154 a         4627 c         4400 g         4191 t
ORIGIN      
        1 tgcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtcatcg gtcagacgcc
       61 aacagtggat aactcggcac tcatcaaaca gatccaacag ttgttgcagc aagaaccgtt
      121 attcagcggt aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg tggatgatat
      181 gctcggcgct accttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccatcc
      241 tggcggctgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
      301 gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc
      361 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
      421 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
      481 ctcggcgcaa cctgtgtgat tgagcgccag aacggcactg aaacgcacga agtagaaagt
      541 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtcgaaggt
      601 gtcctgcccg gcgcgcgtgt ttatgccaaa aacatttcgg cagaagggct gcaaagcggc
      661 aaacagttac cgctcggtcc ggcgttatta ggtcgtgttc tggacggcag cggtaaaccg
      721 ctcgatggcc tcccccgata cgacggaaac cggtgcgctg attaccccgc catttaaccc
      781 gttgcaacgt acaccgattg aacatgtgct ggacaccggc gtgcgcccaa tcaatgccct
      841 gcttaccgtt gggcgtgggc agcgtatggg gctgtttgcc gggtccggcg ttggtaaaag
      901 tgtgctgctg gggatgatgg cacgttacac ccgcgccgat gtcattgtcg tgggtttgat
      961 tggtgaacgc ggacgcgaag taaaagattt tattgagaat atcctcggtg ccgaagggcg
     1021 tgcacgctcc gtggtgattg ccgctccggc ggatgtttct cctctcctgc gaatgcaggg
     1081 tgccgcctat gccacgcgca ttgccgaaga ttttcgcgat cgtggtcagc atgtgttgct
     1141 gattatggac tccctcaccc gctacgcgat ggcccagcgt gaaattgcgc tggcgattgg
     1201 cgaaccaccc gccactaaag gttatccccc gtcggtgttt gccaaattac cggcactggt
     1261 cgagcgtgcc ggaaatggca ttagcggcgg cggatcgatt accgcgtttt ataccgtgct
     1321 cactgaaggc gatgaccagc aggaccccat tgccgactct gcgcgggcaa tcctcgacgg
     1381 tcacattgtg ctgtctcgcc gactggcgga agccgggcac tatccggcta tcgatattga
     1441 agcgtcgatc agccgcgcaa tgacggcgtt gatcagtgag caacattacg cgcgagtgcg
     1501 caccttcaaa cagctgttgt cgagttttca gcgtaaccgc gatctggtta gcgtcggcgc
     1561 gtatgccaaa ggcagcgatc cgatgctcga caaagccatc gccctgtggc cgcagctgga
     1621 gggctatttg caacaaggca tttttgaacg cgcggactgg gaagcgtctc tccaggggct
     1681 ggagcgtatt ttcccgacag tgtcataacc caggagataa cggcagatgg cagaacatgg
     1741 cgcgctggcg accctgaaag atctggcaga aaaagaggta gaggatgccg cgcgcctgct
     1801 gggtgaaatg cgtcgcgggt gtcagcaggc ggaagaacag ctcaaaatgc tgatcgatta
     1861 tcagaatgaa tatcgcaata acctcaacag cgatatgagt gccgggatga ccagcaaccg
     1921 ctggatcaac tatcagcagt ttatccagac gctggaaaaa gccattactc agcatcgcca
     1981 gcaacttaat cagtggacgc agaaagttga cattgccctg aacagctggc gagaaaagaa
     2041 acaacgtttg caggcctggc agacactgca ggaacggcta tccacggcgg cactgcttgc
     2101 agaaaaccgc ctcgatcaga aaaagatgga tgagttcgcc cagcgcgccg ccatgaggaa
     2161 acctgaatga ttcgcttagc gcccttaatt accgccgacg ttgacaccac cacattgcct
     2221 ggcggcaaag ccagcgatgc tgcacaagat tttctcgcgt tgttgagcga agcattagca
     2281 ggcgagacaa ctaccgacaa agcggccccc cagttgctgg tggcaacaga taagcccacg
     2341 acaaaaggcg agccgctgat cagcgatatt cttgccgacg cgcaacaagc ggatttactg
     2401 atccccgtgg atgaaacact gcctgtcatc aacgacgaac aatccacatc aacaccgtta
     2461 accaccgctc agacgatgac gttggctgcg gtggctgacc aaaatacgac aaaagacgaa
     2521 aaagcggatg atctgaatga agacgtcacc gcaagcctga gcgccctttt tgcgatgttg
     2581 ccgggttttg acaatacgcc caaagtgact gatgtgccgt caaccgtgtt accggcagag
     2641 aaaccaacgc tcttcacaaa actgacttct gcgcaactca caacagcaca gcctgatgat
     2701 gcccccggca cgccagctca gccattaaca ccgctggtag cagaagccca gagtaaagcg
     2761 gaagtcatca gcacaccgtc accggtgacc gctgccgcca gcccgctaat tactccacac
     2821 cagacacagc cactgcccac cgtcgccgcg cctgttttga gtgcaccgct gggttctcac
     2881 gaatggcaac aatcattaag ccagcatatt tcgctgttca cccgccaggg gcaacaaagt
     2941 gcagagttgc gtctgcaccc gcaggattta ggtgaagtgc aaatctccct caaagtggat
     3001 gataaccagg cgcaaatcca gatggtttca ccgcatcagc atgtacgcgc cgcgctggaa
     3061 gcagcgcttc cggtactgcg tacgcagctg gccgaaagtg gcattcagtt agggcaaagc
     3121 aacatcagtg gcgaaagctt tagtggtcag cagcaggccg cttcccaaca acagcaaagc
     3181 caacgcacag taaaccatga acctctggcg ggggaagacg acgatacgct tccggttccc
     3241 gtctctttac aagggcgtgt aacaggcaac agcggcgttg atattttcgc ctaacgtcag
     3301 aggtagcacc gtaatccgcg tcttttcccc gctttgttgc gctcaaaacg caggataatt
     3361 agccgataag cagtagcgac acaggaagac cgcaacacat gactgattac gcgataagca
     3421 agaaaagcaa gcgatcgctt tggatcccga ttctggtatt cattaccctc gcggcctgtg
     3481 ccagcgcagg ttacagctac tggcattcgc atcaggttgc cgctgacgac aaagcgcagc
     3541 aacgcgtcgt accctccccg gtcttctacg cgctgaatac cttcacggtc aatttgggcg
     3601 atgcggatcg cgtgctttat atcggcataa ccctgcgcct gaaagatgaa gctacccgct
     3661 cgcggctgag tgagtatttg ccggaagtcc gtagccgttt gctgttactg ttttcgcgtc
     3721 aggatgctgc cgtactggcg acagaagaag gcaagaaaaa cctgattgcc gagattaaaa
     3781 ccacgctttc caccccgctt gttgccgggc aaccgaaaca ggatgtcacc gacgtgctgt
     3841 ataccgcttt tattctgcga taacgacatg ggcgatagta ttctttctca agctgaaatt
     3901 gatgcgctgt tgaatggtga cagcgaagtc aaagacgaac cgacagccag tgttagcggc
     3961 gaaagtgaca ttcgtccgta cgatccgaat acccaacgac gggttgtgcg cgaacgtttg
     4021 caggcgctgg aaatcattaa tgagcgcttt gcccgccatt ttcgtatggg gctgttcaac
     4081 ctgctgcgtc gtagcccgga tataaccgtc ggggccatcc gcattcagcc gtaccatgaa
     4141 tttgcccgca acctgccggt gccgaccaac ctgaacctta tccatctgaa accgctgcgc
     4201 ggcacagggc tggtggtgtt ttcaccgagt ctggtgttta tcgccgtgga taacctgttt
     4261 ggcggcgatg gacgcttccc gaccaaagtg gaaggtcgcg agtttaccca taccgaacag
     4321 cgcgtcatca accgcatgtt aaagctggcg cttgaaggct atagcgacgc ctggaaggcg
     4381 attaatccgc tggaagttga gtacgtgcgt tcggaaatgc aggtgaaatt taccaatatc
     4441 accacctcgc cgaacgacat tgtggttaac acgccgttcc atgtggagat tggcaacctg
     4501 accggcgaat ttaatatctg tctgccattc agcatgatcg agccgctacg ggaattgttg
     4561 gttaacccgc cgctggaaaa ctcgcgtaat gaagatcaga actggcgcga taacctggtg
     4621 cgccaggtgc agcattcaca gctggagctg gtcgccaact ttgccgatat ctcgctacgc
     4681 ctgtcgcaga ttttaaaact gaaacccggc gacgtcctgc cgatagaaaa acctgatcgc
     4741 atcatcgccc atgttgacgg cgtcccggtg ctgaccagtc agtacggcac tctcaacggt
     4801 cagtatgcgt tacggataga acatttgatt aacccgattt taaattctct gaacgaggaa
     4861 cagcccaaat gagtgacatg aataatccgg ccgatgacaa caacggcgca atggacgatc
     4921 tgtgggctga agcgttgagc gaacaaaaat caaccagcag caaaagcgct gccgacgcgg
     4981 tgttccagca atttggcggt ggtgatgtca gcggaacgtt gcaggatatc gacctgataa
     5041 tggatattcc ggtcaagctg accgtcgagc tgggccgtac gcggatgacc atcaaagagc
     5101 tgttgcgtct gacgcaaggg tccgtcgtgg cgctggacgg tctggcgggc gaaccactgg
     5161 atattctgat caacggttat ttaatcgccc agggcgaagt ggtggtcgtt gccgataaat
     5221 atggcgtgcg gatcaccgat atcattactc cgtctgagcg aatgcatcgc ctgagccgtt
     5281 agtgatgaat aaccacgcta ctgtgcaatc ttccacgccg gtttctgctg cgccactgct
     5341 gcaggtcagc ggcgcactca tcgccattat tgccctgatc ctcgctgctg cctggcaggt
     5401 aaaacggttg ggatttgccc cgaaacgcac tggcgttaac ggtctgaaaa ttagcgccag
     5461 tgcttcgctg ggcgcgcgtg aacgggttgt ggtggtcgat gtggaagatg cacggctggt
     5521 gctcggcgtt accgcagggc aaatcaatct gctgcataaa cttccccctt cagcaccaac
     5581 agaagagata ccgcagaccg attttcagtc ggtcatgaaa aatttgctta agcgtagtgg
     5641 gagatcctga tgcgtcgttt gttgtctgtc gcacctgtcc ttctctggct ggttacgccc
     5701 ctcgccttcg cgcaactgcc gggtatcacc agccagccgc tgcctggcgg tggacaaagc
     5761 tggtcgctcc cggtgcagac gctggtgttc atcacctcgt tgacgtttat tccggcaatt
     5821 ttactgatga tgaccagttt cacccgcatc atcattgttt ttggtttatt gcgtaacgcg
     5881 ctgggaacgc cctccgcgcc acctaaccag gtattgctgg ggctggcact atttttgacc
     5941 ttttttatta tgtcaccggt gatcgacaaa atttatgtcg atgcgtacca gccattcagc
     6001 gaagagaaaa tatcaatgca ggaggcgctg gaaaaagggg cgcagccgct acgtgagttt
     6061 atgctgcgtc agacccgtga ggcagattta gggttgtttg ccagactagc gaataccggc
     6121 ccgttgcagg gacctgaagc cgtgccgatg cgcattttgc gcccggccta cgtgaccagc
     6181 gagttgaaaa ccgcatttca gataggcttc acgattttca ttcctttttt gattatcgac
     6241 ctggtgattg ccagcgtgtt gatggcattg gggatgatga tggtcccccc gccaccattg
     6301 ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt
     6361 cgctggcgca gagcttttac agctagagag gcaaaatgac acctgaatcg gtcatgatga
     6421 tggggactga agcgatgaaa gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc
     6481 tggtcacggg ccttatcatc agtattttgc aggccgccac gcagatcaac gaaatgacgc
     6541 tgtcgtttat tccaaaaatc atcgccgtat ttattgccat tattattgcc ggaccgtgga
     6601 tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg tatatcatcg
     6661 ggtaggccgt acaatgatgc aggtgacaag cgatcaatgg ctttcctggt taagcctgta
     6721 cttctggccg ttactgcgcg tgctggcgct catttctacc gcaccaattc tgagcgaacg
     6781 cagcgtgccg aaacgggtaa aactggggct ggcaatgatg atcacgttcg tcattgcccc
     6841 atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca
     6901 gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg
     6961 aaccgctggc gaaattatcg gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc
     7021 ggccagccat ctcaatatgc ccgttttagc gcgtatcatg gatatgctgg cgttactgct
     7081 gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata cctttcacac
     7141 cctgccaatt ggtggcgaac cgttgaacag caatgcgttt ctggcactca ccaaagcggg
     7201 gagtttgatt ttccttaacg ggctgatgct ggcgttaccg ctcattactc tgctgctgac
     7261 cctgaatctg gcattaggtt tactgaatcg tatggccccg caattatcca tttttgttat
     7321 tggatttcca ttaactctga ctgtcggtat ctctttaatg gcggcattaa tgccgttaat
     7381 tgcacctttt tgcgaacatt tattcagtga gatgtttaat ttgctggctg atattattag
     7441 tgaattgcca ttaatataat tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa
     7501 aataatacct acgaacatct tccaggatac tcctgcggcg aaatatttgt tttaagctca
     7561 ctcacatatc gcaacattta ctttaagaca attccaggca aattatacaa cactttacgg
     7621 gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg
     7681 tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga gtgagggtat
     7741 gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt aaccgggtat
     7801 ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt
     7861 gccatagctt gtgattcaca gcgcccttca gtggtgttta ttaatgagga ctgtttcatc
     7921 catgatgctt ctaacagtca gcacatcaag cacatcatta atcaacatcc caatacgtta
     7981 tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt ggtcagaaaa
     8041 aatttattga taagttctaa atcgattaaa ccggaatctc tcgacgatat ccttggcgat
     8101 attctgaaaa aagagacaac gataacctct tttttaaata tgccgacgtt atcattgagc
     8161 cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat tcaaatctct
     8221 gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat taaacgtaag
     8281 atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga taatgtgact
     8341 aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc accagtcagg
     8401 ctgaataaga ttactctgct ttctccacaa agataccgtc ctgatgccct gcttcattaa
     8461 agaaccagat gccgagcggg tagtcttcca gcgaaaccag gtacattgtg ccttcactaa
     8521 actcctcaac tgctagtacc acgccaggac gacgcggacc gccatccgtt ttgactgtta
     8581 cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta gaacaatttc
     8641 gtttttctgg cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag
     8701 agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc gaggaaagat
     8761 gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga
     8821 tgccctgctg gcggcgatca atgaaataag ttcatcgcag ccagaacgat tctgaacacg
     8881 ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc ttcgaactgg
     8941 atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa aaaatcccga
     9001 ccctgagggg gtcgggatga aacttgctta agcaagaagc acttaaaaaa ttcgttacac
     9061 caggaaatct gatgtgttca tcaccttatc cgcaattttt ttcgctgaca agaaaatatt
     9121 cgcgatatga atgattaacg ctacttatga atagaaatgt gacgtgcgtc accccttggt
     9181 ggctctggcg tcaacgggat cgctcccgaa aaaatcatct ttgattattt gtgctgtttt
     9241 gagaatcatc ctggtaaggg gagaactgcg cgaccatgtt ttcaattcaa caaccactac
     9301 tggtttttag cgatcttgat ggcaccctgc tggacagtca tagttatgac tggcaaccgg
     9361 ctgcccccta gctcagccgt ttacgcgaag cgaatgttcc cgtcattctc tgtagcagta
     9421 aaacttcagc ggaaatgctg tacttgcaaa aaacgttggg gttacaaggg gtaccactga
     9481 ttgccgaaaa tggcgcagtg atccagcttg ctgaacaatg gcaggatata gacggttttc
     9541 cacgcatcat ctcaggtatt agccatggcg aaatcagcca ggttttaaat acgctacgcg
     9601 agaaagaaca ttttaaattc acgactttcg atgatgtcga cgatgcaacc atcgccgaat
     9661 ggacgggatt aagccgtagc caggcggcgc tgacgcagct gcatgaggcg tcggtaacgc
     9721 taatctggcg cgacagtgac gagcgtatgg cacaatttac cgctcgtctg aacgaactgg
     9781 gcttacagtt tatgcagggt gcgcgcttct ggcacgtcct ggatgcctct gccggaaaag
     9841 atcaggccgc caactggatt atcgcgacct atcaacaatt atcaggcaaa cgcccaacca
     9901 cacttggcct gggcgatggg ccaaacgatg cgcccttact ggaggtaatg gattacgcgg
     9961 tgattgtgaa agggctaaac cgtgaagggg tgcatctgca tgatgaggat ccggcccgcg
    10021 tctggcgaac gcagcgtgaa ggaccggaag gatggcgtga agggctggac cattttttct
    10081 ccgctcgtta agcgttatcg ctcgcgcata cccgattacg cccggcctgt ttagcgagat
    10141 aaagccgacg gtcagccagt gactggagtt gttcaaaatc ataatcaccg gcttcctcgc
    10201 tgctacttac ccccaacgag gcactgatgc gtatcgtcgt actcttagca atcaacatct
    10261 ctttttcatt taacttcagg cgaatacgtt ctgcgacttc cgcagcctcc gtcagactcg
    10321 cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaacg tcctgcgcac
    10381 gcaaggaact gctaattaat ccggcagcat gagaaagaac acggtcgccc gcctgatgac
    10441 taaagcggtc attaatcgct ttaaaatggt caaggtcgac ctgaatgaca gaaaaaggat
    10501 gttggtgcgt ctgacacaat ttcgcgagcg gacgagcttt ttcaaacagt gcgccacgat
    10561 tatataaacg ggttaaggtg tcgtgccacg cctgccactg caacgagctt tgcagaacat
    10621 acatgttgct aaccatccgg cgaatcacat accaggagat gagtaacatg gtggtaaaga
    10681 gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc acgccttcgc
    10741 ttagcgtatg gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc cagctaacat
    10801 agcgactgtc catacgaatg ccgccccgcg tgtcatgttc catcgcctgc gccagcaagg
    10861 ccagttcacg aggatcaaaa atattccctg ttggatggtc aggattggaa gaggtcaaaa
    10921 atctcagctt actgtcatag agctgatact caccatcgag gtttttatcg atggcgtttc
    10981 ttaaaaattg ctgcatggta cgcacgggaa tactcatccc cagcacgcca taccagtagt
    11041 tattactgtc taccggaaca ctgacggtaa cctgcggttc agtattgctg gcgtgttccg
    11101 gttgcgaagt aaaccagcgt accgcgcggt gacgattttc tcattgcgaa tggccgataa
    11161 accagggttg ggtgacatag ccgtaataac gcgttggtac attgcgcgta aacaaggtcg
    11221 gctgcgtcga aacgtaaaat ccggcacgcg agacatacat cgcctgttca accatcgacg
    11281 aggtgttgtg cgccagtcgc agcaagtaac caacttccag tgcagcggta atttcattgt
    11341 cgaggctttc attttcgcga gacaggagat tcccctcgct gactaatgca tccgatacgc
    11401 cattgactga cagggtgcgt cgtcggttga gttcaatttg ccaggcgtgc tcgtcgcgat
    11461 gctgttcgaa ctcggttaca gcattacgca gtgaagtgaa atccagtggc gctacgagag
    11521 cttcgcgcat gccattacgc aaaaagatca gtttgtcgac gttatactgc aaatgcttat
    11581 cgagcgcgtt ggctacgttt tccagatgat tacgctgact ggagatatag gcatcttcca
    11641 gcactaccac ttcacgccag gtcagcaagg tggaaaaaag caataccacg ataaagcaga
    11701 gattaacgac atgaccaggc cccaggcggc gtgcgatttt tttcaaccag ctctggtttt
    11761 ccatttttgt ctcgtgctgc accctgacga ctcccaacct gcttttttat gattctggca
    11821 taacctggcg atagcgtacc gcaaaacagc gataatttta taatggtggc atgataagac
    11881 gcgactatca ttcattgcag gcattggcac accaggccgg aagtgatccg gcctggtgtt
    11941 aatgataccc tttcaaactt cttccgcaga acgactcccc tcccccggaa caagctcggt
    12001 ttcatgaaaa gcttcccgct tgaccccgta accgtcatac caacgacact caaccatacc
    12061 gctggagtat ccggtgacaa tcatccgcgg gccgccctct ttaaccgtaa cttcctcact
    12121 aaccataaag ctcatactcg cctccttttt tcgagtgaaa cttgttcacc ttagttgaag
    12181 atggcgaatt ttgcataata gcctgtgcga gtattaatgt gcctgaccgc gtattttcgc
    12241 tacggctttc actcccagca ccacgatgcc gccgatgata aatccaagaa tcagatttaa
    12301 aacagtcggt aatatcatcg ccaccactgc actttgctgc ccggcgaaat gttcaatggc
    12361 gtgatgcagc ggcgcaatac catgtaccac aatcccgccg ccgacgagga acatcgccag
    12421 cgtgccgaca atcgataacg ctttcatcag ccagggcgca atgatcaata accctttacc
    12481 taatgcctgc atcagcgcgc tggatttttc cgccagccaa taccccaggt catcaatctt
    12541 aacgataacc cctaccagac cgtagacgcc cacggtcact accagcgcga tgcctgaaag
    12601 caccagcacc tgattaagca acggcgcttc ggccacaatc cccagcgtga tggcgacgat
    12661 ttcggcagac aatataaaat cggtacgaat cgcccctttt attttgtcct tttcaaactt
    12721 cagcggatcc tgcgccgcca gcttctccag acgctgctgg ctctgcgccg gatcttcttt
    12781 atgtttacgc gcctccagca tatgcagcac tttctctact ccttcaaagc agagaaacgc
    12841 gccaccaatc atcaacagag gcgtaatcgc ccacgggata aacgcactga tgatcagcgc
    12901 cagcggcacc agaatcactt tattaatcag cgatcctttc gccacgcccc agaccacggg
    12961 aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata agtcatcccc
    13021 taatacaccg gcggttttct tcgccgccag tttgcccatc acggagatat cgtccagtag
    13081 tgtggcgata tcatcgagca acgtcagtaa actactcccg gccaaaagca ttccctctca
    13141 tttttattgt tgaagggtgt aagtatgaag caaaaacggc agctctgata cttgcttcac
    13201 aggtcaacat ttttttaaca atcaagtgca attatatctc tcgccagcag aatgattttc
    13261 acgtttacta tgaaccacct ttttatttcc accgtgaggg attatgcgtt tccgccagtt
    13321 gttaccgctt tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa cctattttgt
    13381 cattcggatt ggcgtggaaa gctggcctcc gttaatgatg gcaggcgttc gattcctggc
    13441 agccggtatt ttattgatgg catttttgct actgcgcgga cacaagctcc ccccgctacg
    13501 tccgctgctc aatgccgcgc tgattggcct gttattgctg gctgtcggta atggcatggt
    13561 gacggttgcc gagcatcaaa atgttccttc cggcatcgcc gccgttgtgg ttgcaaccgt
    13621 gcccctcttt accctgtgct tcagccgcct gtttggcatt aaaacgcgca aattggaatg
    13681 ggtgggtatc gccattgggc ttgccggaat catcatgctc aatagcggtg gaaatttaag
    13741 cggcaatccg tggggcgcga ttctgatttt aatcggctcg attagctggg cgtttggctc
    13801 ggtttatggc tcgcgcatta ccttacctgt agggatgatg gcgggtgcga ttgagatgct
    13861 ggcggcaggc gtggtgttaa tgatcgcgtc gatgattgca ggtgaaaaac tgacggcgct
    13921 cccttccctt tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt cgattatcgc
    13981 catcaacgct tatatgtatt taatccgtaa tgtcagtccg gctctcgcca ccagctacgc
    14041 ctacgttaac ccggtggtcg cggtcttgct gggtacggga ctgggcggag agacgctgtc
    14101 gaagattgaa tggctggcgc tcggcgtaat tgtcttcgcg gtggtactgg tcacgttggg
    14161 aaaatatctc ttcccggcaa aacccgtagt tgcgccagtt attcaggacg catcaagcga
    14221 gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag atccactctt
    14281 ccagacgctc ggtaagcgct tcatccgtca gcttctcgcg cccacgtaat gcacactccc
    14341 agacaatcag tacgcgccag ccgagttcct gcaaacgact gatatcgcgg cgatcgcgtt
    14401 caacattttt acctatcttc tccagccaga actcggttcg agtcgcaggc actttaaaca
    14461 gatagcagtg atgatgatgc cagaagcagc catgggtaaa tatcacgcag cgatattcat
    14521 caacgacaaa atccgggctt ccgggcagac tggcgtcctg aacgcgaaac gccaggccct
    14581 gcccggttaa cagactggcg aggcgcttct caatcgccgt atcacgcgtg gcaatcgcgc
    14641 gcatattttt gctgcgagtg gccttatcgt gaacgtcggc catgttgtgc ctcttgctga
    14701 cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc ggcaaagacc
    14761 ggcacgacca ccgagttacc gaactggcga taggcctgag tgtccgaaac cggaatgcgg
    14821 aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg cgcattcccg aggcgttaac
    14881 cgacgtggac gatgttgctg attcagcgga tcgtcaaagt ctttctcacc tttggccata
    14941 tcccagccgc gatcgattaa aatttccgcg ccatctttgt aataacgcgc agacagcgta
    15001 cgcgtgacgc tttgcggatt gttcggataa accattccat aaccgaagcc gttaccgcgc
    15061 gcctgatgtt ttttcgcata tcgatagagg tacttccaca gcaccggcgt caggatatat
    15121 ttcgcctcga ccatcgggtc caacagctgc gccagcgtca ctcgctgtgc agggaaacat
    15181 tcgctgatat cacgcagagt aaaatcggct ttcagattaa gatcgcgacg aaaacccacc
    15241 agcacgatgc gttcacggtg ctgcggcaga aaatgtttac catcgatgat tttcggatcg
    15301 tccggcccgt tatcttctgc atcagccacg tcatagccca gttcgtccag cgtctgcatg
    15361 atgatgcgga acgttttacc ctggtcgtga cttttcaggt ttttgacgtt ttcgagcaca
    15421 aacatcgccg gacgacgcgc gtcgataatg cgtaccacat caaaaaacag cgtgccctgg
    15481 gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct ttttcgatac gccagccagc
    15541 gaaaatggct gacaagggaa accggccagt aaaacatcgt gttcaggaat gtgttgacga
    15601 atatgttccg ccgccgcctc atcactcacg ccttctttat ggctgagggt gatatcgcgg
    15661 atatcttcat taaaatgatg cgtcgccgga tcgcaataat ggttggcttt ataagtgcgt
    15721 accgcatgtt tgttccattc gctggtaaac acgcattgtc cgccaatcga ttcaaaaccg
    15781 cgacggatgc cgccaattcc ggcaaataga tcgataaagc gaaacgcata atgcggatga
    15841 tgtgccggtg gtttgggtaa caacgtttgc agatgggcga actctttctc acttaaacgg
    15901 tgccatgccg agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca gtgattctca
    15961 cccacaccat taagctgcgc caccagcgtt ttaacatcat aaatttgcag cagtttctcc
    16021 agcattgcct gtgcggcatt cccggtgctg tatgaatcgg ttactgatat attttcctgc
    16081 atagatttaa ccggccatct gagatggccg acaggttaac ataattacag ccatttaggg
    16141 ccacgcgctt atcccgcggg tgaaaacgca tctatcacct tgtgatcgac accttcattc
    16201 tcccccgcca gttcggcact gagcttcgcc ataaactcga ctaaaaaatg cgcgttgtgc
    16261 tgcgccagct gcttgcctct tgcggtttgc atggtttgca tggtttgcgg tagcttcagc
    16321 aacttagtct ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg atgctgtgcg
    16381 aacggatctt caccatcaaa cagcgccacg cccaatgccc ctgaaacggc aaacacgcgc
    16441 gccaggccaa tcgcccccag ggcttccagc cgatccgcat cctggacgat tttagcttcc
    16501 gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg caatgacatg acaaacggcc
    16561 tcgattttct ccgccggaaa ttgctcgaac tcttcacgga gcaggcgacg cgtctcttct
    16621 gccgccagga ttgaagaacg ctgcctttgc ggatgatttt tcgccaggct cacaatatcg
    16681 tgaaaataac aggcggttaa aatcaccagc atatcgacat cgtcatctgc cgccagcttc
    16741 tgtgccgtag cccagacgcg gcgaaaatga cacacgtcat gggccgcgtc ctggtgctga
    16801 tgatggtttt tcaaccagtt ttcgaactgt gcctgccagt gttgtaagtc catacgcgct
    16861 ccgttgccaa agagtcgcca ccttagcagt ttttattcat agcgtctgct acgtgcggga
    16921 cgaggtcggg tgtaccaggc aatgctgcct aacaatgccc cgaccagagc cataaataaa
    16981 aatccgacca gtgcccctaa ccactttccc gcggtagagc ctaaatcgct ttcaaatccc
    17041 gacacggggg catcggggat ccggctcatc acccagatgt aacgcgccat tgcccagacg
    17101 aaatagccac accatgtata gaaagcccac aaagccagtt tgccgccagg gctgcgagag
    17161 agtctctctt ccatctttgt cgaaccgtat tccattttga gataacccgg aaaattcaga
    17221 cttagcacta ttatgtgata aaagtcacat ttttccacat tggatgagcc agtcaagact
    17281 caatcatttt ttgaacaaat agataaattt tgcgcaacgg cagtgaatac aggaaaaatc
    17341 gttttgagcg attttattta tctttgatat cg
//
LOCUS       DAJTYD010000073        17115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTYD010000073 DAJTYD010000000
VERSION     DAJTYD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.073"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..2429)
                     /gene="topA"
                     /locus_tag="OO585_002200"
     CDS             complement(<1..2429)
                     /gene="topA"
                     /locus_tag="OO585_002200"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6629781.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPE"
     gene            2809..3060
                     /locus_tag="OO585_002201"
     CDS             2809..3060
                     /locus_tag="OO585_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6629782.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3096..4145)
                     /gene="sohB"
                     /locus_tag="OO585_002202"
     CDS             complement(3096..4145)
                     /gene="sohB"
                     /locus_tag="OO585_002202"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6629783.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4365..5123
                     /locus_tag="OO585_002203"
     CDS             4365..5123
                     /locus_tag="OO585_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6629784.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5120..5711
                     /gene="cobO"
                     /locus_tag="OO585_002204"
                     /pseudo
     CDS             5120..5711
                     /gene="cobO"
                     /locus_tag="OO585_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5751..6623)
                     /gene="rluB"
                     /locus_tag="OO585_002205"
     CDS             complement(5751..6623)
                     /gene="rluB"
                     /locus_tag="OO585_002205"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6629785.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(6834..8729)
                     /locus_tag="OO585_002206"
     CDS             complement(6834..8729)
                     /locus_tag="OO585_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCR6629786.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8757..9377)
                     /locus_tag="OO585_002207"
     CDS             complement(8757..9377)
                     /locus_tag="OO585_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6629787.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9374..10255)
                     /gene="yciV"
                     /locus_tag="OO585_002208"
     CDS             complement(9374..10255)
                     /gene="yciV"
                     /locus_tag="OO585_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6629788.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10393..10437
                     /gene="trpL"
                     /locus_tag="OO585_002209"
     CDS             10393..10437
                     /gene="trpL"
                     /locus_tag="OO585_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6629789.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10414..10507
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10529..12091
                     /gene="trpE"
                     /locus_tag="OO585_002210"
     CDS             10529..12091
                     /gene="trpE"
                     /locus_tag="OO585_002210"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6629790.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12091..13686
                     /gene="trpD"
                     /locus_tag="OO585_002211"
     CDS             12091..13686
                     /gene="trpD"
                     /locus_tag="OO585_002211"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6629791.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13687..15048
                     /gene="trpCF"
                     /locus_tag="OO585_002212"
     CDS             13687..15048
                     /gene="trpCF"
                     /locus_tag="OO585_002212"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6629792.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFNPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15060..16253
                     /gene="trpB"
                     /locus_tag="OO585_002213"
     CDS             15060..16253
                     /gene="trpB"
                     /locus_tag="OO585_002213"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6629793.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16253..17059
                     /gene="trpA"
                     /locus_tag="OO585_002214"
     CDS             16253..17059
                     /gene="trpA"
                     /locus_tag="OO585_002214"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6629794.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4114 a         4580 c         4296 g         4125 t
ORIGIN      
        1 ttttccggca gacggtcgcg gaagcgataa agctcttcca ccagtggcgc acgcgtttca
       61 cgcgatttcg ggaaagtgtt ggcagccagg aaaacacccg cagcgccgtc acgcagcacg
      121 aaataagcat ctgatttttc gcacggcagc tcaggtaatg gcaccggatc ttctttcggt
      181 ggtgccactt cgccgttacg taaaatctta cgtgtgtttt tacactcttc gttggtgcag
      241 gccatgtatt taccgaaacg ccccattttc aggtgcattt cagagccaca tttttcacac
      301 tcaacgatcg ggccgtcata acctttaatg cggaattcgc cctcttcgat ctcgtaaccg
      361 tcgcaggttg ggttattacc acagacatgc aacttacgtt tcggatcgat gagatagctg
      421 tccatcgccg tgccgcattt cgggcaacga cgttttgcgc gcagcgcgtt ggtttcagcg
      481 tcttcgcctt ccagtacgtt cagcacttcg ttttccggca ccaggttaat ggtggttttg
      541 caacgctctt tcggcggcag cgcataacca gaacagccaa ggaatacccc ggtgctcgct
      601 gtgcgaatcc ccattttgcg accacaagtc gggcagtcaa tgctggtcag aaccatctgg
      661 ttcgggcgca taccaccctc ttccggatct ttttcagctt tatctaactg ctgggtgaaa
      721 tccgagaaga agtgatccag tacagctttc cactctgctt cgtgatttgc cacctggtcg
      781 aggctgtttt ccatctgtgc ggtgaagtcg tagttcatta actcgcggaa attttcttca
      841 aggcgatcgg tgacgatttc gcccattttt tccgcataga aacgacgatt ttctactcgc
      901 acgtagccac gatcctgaat ggtcgaaatg atcgacgcat aggtagacgg acgaccgata
      961 ccgcgttttt ccagctcttt aaccagcgat gcttcactga aacgggctgg cggcttggta
     1021 aagtgctggg ctggtgtaag ttcaacgagc gtcagagcat cgcctttatt aactgctggt
     1081 aagatgcgat cttcatcgcc tttacgcaac gcaggcatca cttttgtcca gccatcaaaa
     1141 cgcaaaatac gacctcgtgc tttcaggcgg aaatcgcccg caccaacggt cagcgtcgtg
     1201 gagtcatatt tcgctggggt catctggcag gctacgaact gacgccagat taactggtac
     1261 agtttctgcg catctgcttc catatccttc agcgattccg ccatcacatt gacgtcagaa
     1321 ggacgaatcg cttcgtgcgc ttcctgtgag ttttctttgc tggcgtactg attcggactt
     1381 tccggcagat atttcttacc aaaattatcg ctgatataac cgcgaaccat atttaccgcg
     1441 tcctgactca ggttagtgga gtcggtacgc atgtaagtga tatagcctgc ttcatacaaa
     1501 cgctgcgcca tcatcatggt ttttttcacg ccaaatccaa gacgggtgct ggcagcttgt
     1561 tgcagcgtag aggtaataaa aggagcgcca ggtttactgg ttgtcggttt gtcttcacgt
     1621 tccagcacgc tgtagcgcgc tttttccagc agacttaccg cagcctgagt ttgttctttg
     1681 ttgaccggac ggaacggttt gtcgttctga tgagtcacct gtaacgccaa cgcttcacca
     1741 gatggcgtgg tcgtgctggc atcgacttcc cagaactctt ccggcacgaa cgctttaatt
     1801 tcacgctcac gctcgaccac caggcgaacc gccaccgact gcacacgacc ggcagacaga
     1861 ccacgagcga tctttttcca tagcagcggc gaaaccatat accccaccac gcggtccata
     1921 aagcgacgcg cctgctgggc attaacacga tcaatattca gctcacccgg tttgttaaat
     1981 gcctggcgga tcgcgttttt agtaatttcg ttaaacacca ctcggctata gcgcgcatca
     2041 tcacccccaa tcacttcccg caggtgccat gcaatggctt ccccttcgcg gtcaaggtcg
     2101 gttgcgagat agatgtggtc ggctttttca gccagttgtt tcagttcaga gacgaccttc
     2161 tctttaccag gcaacacttc atagtgcgcc tcccaattgt gccacgggtc aacccccata
     2221 cggttgacga gagcgccacg ttcatccttt ttaggctttt tagccgtctt ggtggaggta
     2281 gagtcggcac tctttttggc agctgagcca ctggtcggca aatcgcggat gtgaccgacg
     2341 ctggatttca ccacgtagtc actacccaga tacttgttga tcgttttggc ttttgccggg
     2401 gactcaacga tgacaagagc tttacccata ttcaccttac ctaatttgat tcttccagga
     2461 atgcgtcgca cgttgataaa ccttccactg gcaacgagtt accgatatgg actcgttctc
     2521 tgcggatatc aaccccttgt cgtgactctg ttcacgaaaa ttcaggtgac tgagttaaca
     2581 ggtcttggcc tacaggcgct atagcacgac ggaattttgg tcgaatgtca agcaaatcta
     2641 ttgccagatt gacgaaagcg tcacactgta cctgataaaa tccgatacgc aactttatta
     2701 gcatgcaaaa tccaaagttg ccagtaaggc gctcttgcca ccagcgtggt atatttgtct
     2761 gcaagccatg cctggcgtag actaatgtcc ttatttaagg agtaaataat gaacaaagag
     2821 acacaaccca tcgaccgcga aacgctgttg aaagaggcca acaaaatcat tcgtgagcat
     2881 gaggatacgc tcgcagggat tgaagccacc ggcgtcacgc agcgtaatgg cgtgctggta
     2941 tttaccggcg actacttttt agatgaacaa ggattgccaa cagccaaaag caccgcggtg
     3001 tttaatatgt tcaaacacct ggcccatgtg ctttcagaaa aatatcatct ggttgattaa
     3061 agcaaaacgc gaggtcttag cctcgcgttt gtcttttaca tcaatggctt ttgaccccgc
     3121 tgccaccagc gtagcaacaa tcgatcggcg ctctctgccg cgctgccggt gaatcggtca
     3181 atgagtcgtt tacgctgcat atagcgtaca ttaaccactt cacggccttc catcaggcta
     3241 agaataactt catcactggt gttgatttca tcaaccaggc ctttctctac cgcctgttgt
     3301 ccgtaccagt gttcacctgt tgccacctgt tcaatatcca gagacggacg catacgcttc
     3361 acaaaatctt taaacaactg atgcgtttcg ttcaactctt cgcggaattt ctcccgccct
     3421 tcttcggtat tttcacccag caacgtcaga gtacgcttat actgcccggc agtgtgcagt
     3481 tcgatatcaa tatatttgct tttcaggaag cggttaaagt tgggcatttg cgccaccacc
     3541 ccaatggaac ccacaatagc aaacggtgcg gaaacaattt tgtccgccac acaggccatc
     3601 atgtaaccgc cgctggcagc gactttgtct accgtaacag ttaaaggaat gtttttatca
     3661 cgcagacgct gcagctgcga agccgccaac ccgtaaccat gcaccatgcc accagggctt
     3721 tccagacgga gcacaacctg atcctgcggt ttgaatgctg cgagtaccgc cgttatctct
     3781 tcacgtagcg agttcacttc atgggcgtcc atgctgcctt taaaatccag cacccagacg
     3841 cgaggtttac tgtcagttgc cacctcgccc agtttggctt tcgcttttgc tgctttcgct
     3901 tcttgcttgt gctttttctt ctgcgctttg tgccactgtt tttgctgatg tgagcccatc
     3961 agcgccgcgg ccagttcttc tttcatctcc ttatactgtt cgctgagatt gttgacccgt
     4021 aactcgccac gctggcgttt attacgttga gcaacattga caataatggc ggcaatcgcc
     4081 gcaatcgcta gcacaacggt aacgattttc gccaaaaaca aaccatattc agacaacaat
     4141 tccacgagtc cccaccttgg ttaaacaacg ctttggctca cagtttacaa caggcatgtt
     4201 aagccgtctc gcagagatgt tattgcagcg aaagtatgac aaagtatcca ttttgcggtg
     4261 ttatcgcccc aggctgattg aaatgccctt ctgtttcagg cataaagccc caaagtcata
     4321 aagtacactg gcagcgcgac agacaacgtc gttgaggagt agccatgcat taccagccaa
     4381 aacaagattt actcaatgat cgcattatcc tggtgacggg agccagcgat gggattggtc
     4441 gtgaagccgc gatgacgtat gcacgctatg gtgcgacagt gattctgttg ggccgtaatg
     4501 aagaaaaatt acgtcaggta gccagccaca taaacgaaga aactgggcgt cagccacagt
     4561 ggtttattct cgatttgctg acctgcacgt ccgaaaattg ccaacaactg gcacagcgca
     4621 ttgtcgttaa ttatccgcgt ctggatggtg ttttgcataa tgccggattg ctcggcgata
     4681 tttgcccaat gagcgaacaa aatccgcagg tctggcagga cgtcatgcag gtcaacgtta
     4741 atgccacctt tatgctcacc caggcactgc ttcctttatt actcaaatcg gacgccggtt
     4801 cactggtctt tacttcatca agcgttggac gtcagggacg agctaactgg ggtgcatatg
     4861 cagcgtcgaa atttgccacc gaagggatga tgcaggtact ggccgatgaa tatcagcagc
     4921 gcctgcgcgt caactgcatt aacccaggcg gtacgcgcac cgcaatgcgc gcaagcgcct
     4981 tcccgaccga agatccacag aaacttaaaa cacccgctga tatcatgccg ctctacctct
     5041 ggctgatggg cgatgacagc cgccgtaaaa ccggcatgac ctttgacgcc caaccgggcc
     5101 gtaaaccagg aatttcccaa tgagtgatga acgctaccaa cagcgtcagc agcgagtgaa
     5161 agaaaaagta gatgctcgtg tggcacaggc cccaggatga acgcggtatt atcatcgtct
     5221 ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg tacggcaaca cgcgcagttg
     5281 gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg cacctggcct aatggcgaac
     5341 gcaatctgct ggaaccacat ggcgttgagt ttcaggtgat ggcaacgggc tttacctggg
     5401 atacacaaaa ccgcgagtct gataccgccg cctgccgcga agtctggcaa catgcaaagc
     5461 ggatgcttgc tgattcctca ctggatatgg ttttgcttga tgaactgacg tatatggtgg
     5521 cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt aaatgaacgt ccacatcaac
     5581 agacggtgat tatcacgggt cgaggctgtc atcgggatat tcttgaactg gcggatacgg
     5641 taagtgagct gcgccccatc aaacatgcgt ttgatgccgg tgttaaggcg caaataggga
     5701 ttgattatta atctgtaaaa atgccggata tgacgtccgg catactttga ttaaccgtta
     5761 ttacgcccgc cagaacgacg accgccgctc acctgactgt gacgtttcac cgcacgacga
     5821 atctgattag ccttcatgcg acgacggtct ttttctacag cgactttaga gctggtttct
     5881 ggcggaagct ccaccagttc gcgcagatag ttagtctggg cgaggtccag ctccgtccag
     5941 ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt aacgaacacg gatcaggcgg
     6001 ctcacctgca caccaaccgc ttcccacaga cgacgaactt cacggttacg gccttcggtc
     6061 agagtcacgt tgtaccactg gttgatccct tcgccgccgc tgaacttgat ggttttaaaa
     6121 gctgccggac catcttccag ttgcacgcca cgactcaaat cacgcagttt cgcgtcgtca
     6181 acctgaccaa atacacgcac ggcgtattca cgttcaactt cacggcttgg atgcattaaa
     6241 cggtttgcca gttcaccatc ggtggtgaac agcagcagac cacaggtgtt aacgtccaga
     6301 cgacctacgg caatccagcg tgcgccacgc agctttggca gacggtcaaa caccgtcgga
     6361 cgcccttccg gatcgttacg ggtacacaac tcaccttccg gtttgtaata ggccagaacg
     6421 cgacaaattt gttcagcgga ctcacgtacc gaaatcagat gaccatcgat acgaattttc
     6481 agcccagggg taacttcaac gcgatcgccg agtttagcaa ttttgccatc aacactcaca
     6541 cgaccggctt caataataga ttcgatttca cggcgggagc cgtggccagc gcgggccagc
     6601 actttctgta atttttcgct catagagctt ccttttggtg tcgccttccc aggcgtctga
     6661 tatgcttttg tgcaacagtg agtcagaact tacatctgac tcactgatag taaagtctgt
     6721 ttgaactctt caggccttaa gtaaacgact cgacgtttat cggcctggct ttgaggcgca
     6781 acatactaca ctatctttat ggttaaagct tgtttcgatt aacatcagct taattaccag
     6841 ccaccaccgc ctccgccacc cgcgccgccg ccagcaccac caccggaata acttcgcccc
     6901 aacaaactgg tattggcccc gcaacttagg tgcgtctgta atgagaataa aacgccgtta
     6961 cgggcaatct ctggagcgcg aatcgccgaa gaaagcgaag gggcaatgcg taacgcccat
     7021 tccctcccca aatttgccga tatagcccaa cctagcaacg cgtaatccag tttccgtgtt
     7081 tcccctttcc gccgacgtcc actgtgtgtt gctgcctctt tacgcgcaag atagttaacg
     7141 atagcttcac cgcgggcata acgttgctgc ccagtttggg taaactttgg cagaaaaata
     7201 taccccatgc cagtgagata accggtcagg caaacagcgc taaaataacc cgctggcata
     7261 taccagtaaa agaatacatc tccgacattg atatagagag aatagaaggc cacgcctgaa
     7321 caaatcagtg gtaaaaacaa agtgatgatt gcacgattaa cgcaaggctt gtcatcatcg
     7381 cgggcaaaca aaagctcaag ggaacaaaat aaaaaaggaa taatccagca gatcataata
     7441 aacatatcgc ctaccagact cattcctgcc caacctacgg gactcaggtt accgtagaga
     7501 atgatcgcca atattgccag tccaccccat tgcaaaaacg tatcaggccg gtaaaacaaa
     7561 gactttttac gcggcagata aaatttctgc atccgtaaaa atgccctgcg taagactctg
     7621 ttgcgtctcc ccgtaagaac gaccttatcg cctttacgaa ataacgcttc cagtaataac
     7681 tgctctgcgg gcagtaatac ctcattattt ctggtcgcgc cttcattaac acaaatcagg
     7741 atttgctggt tcttatcata ctgatcttcc agatgaattt ttcccttcac gatcagattt
     7801 acgatatcgg cacagaaacc tttatcgtcg taaaccagct tcgcatcgag acgtaacatg
     7861 ccgggcgtgt aatctggcgg aatgacatcg gtttcaatca catctaccgg cgtaaattgc
     7921 ggcctgcgct tccagagata taaccatcca caaaccagga atagacacgg aaaccaaatt
     7981 aacaacgatg acgtggaggg taaaagaaga tggctgaaaa tattcgtcgt ttgtggagcc
     8041 ggggcattgc taagtaacgc gtgaggccag cggtagagta ccgtgaaatc ttcacgataa
     8101 aacggatcgc gggattctat tcttccgtcc tcaaggatgc ggccatttcg atatgtgtcg
     8161 ccctcttctc cagtaaagag atcgatttcg ctaaatggat tacccgcgat atcagggaac
     8221 ttgagtgaaa atcgggtctt atagatttca aacggccagt ggttaccagt cacgttccag
     8281 attaacagat ctgaatctcc ctcacgcagg aaagcatttt taacctgata atgaatttga
     8341 taatgatagt cgccttgagg caagaaacgt tgtttatcgc ccacgacaat actcattaac
     8401 ccgatatgcc agtcaagatg ccagggttca ggaataccat cacgagtgac gccaacaata
     8461 tgatagttca gcagtgcagc gtccccatca ggctgcatcc agagtcgtgg taaatcacga
     8521 taaaatccgt gcttaatctg ctgatttcgc gcctgaaaat gaatattttc tttgatgtcg
     8581 agcaccccat ccggttgcag agtggcttca acatcaaaga gcgagatccc ttcttcataa
     8641 gaagggatct ccgggttttc tgcataactt agagatgata caaacagccc tgctatcaac
     8701 agcaaaatgc agcgaaactt ccctgccata gtcttccatc cgtggaacgc aatttgtcat
     8761 aagaaaggct tcacatcacc cacgccttca cgcaccacga ccggcgtatc atcggtaaga
     8821 tcaatgaccg ttgtcggttt ctgtccgaga tagccgccgt ggataatcaa atccacctgt
     8881 ttttccagac ggtctttaat ttcttctgga tcggattcgg taaattcgct gcctggcagc
     8941 attagcgaag tggaaagcat cggttcgccc agtgcttcaa gcaacgcctg cgcaatcggg
     9001 ttagaaggca cacgcatacc gatggttttg cgtttttcct gcaacagacg gcgtggcacc
     9061 tccttcgtcc ccttcaggat gaaggtatag ttgcccggcg tgttgttttt cattaaacga
     9121 aacgcaacgt tatccacgaa tgaataggtc gaaagttcag aaagatcgcg acacatcagg
     9181 gtaaagttgt gaccgtccgg cagctggcga atacgacaaa tacgctccat cgcgttttta
     9241 tcttcaattt tacagccgag cgcatagccg gaatcagttg gataaacaat caccccgcct
     9301 ttacgcacga tctccaccgc ctggttgatc agacgttgct gcgggttatc aggatgaata
     9361 taaaaaaact ggctcataat tccctctctg tggtgttctg cggctgttcc catagctgcc
     9421 agacgccttc aacgcctgcg ggcagccaga gtttacgacc cagttcgatc cacgggcatg
     9481 gctgatgaaa atcagatcct tgtgatgccc ataaatgatg ctgacgcgca agtgtcgtca
     9541 actgggtgcg ttcattgggc gattgctggc actgcgcgac ttccatcgcg tcgccgtggt
     9601 gttcggcaaa atgcgcaacc aatcttttca gccatttagc actaagattg taccgcccag
     9661 gatgagctag caccgcctta ccgccagaat gatgaatgac atcaatagct tgttctattg
     9721 tacaccactg tggcggaacg tatccggttt tcccgcgcgc cagatacttt ttaaagacat
     9781 ctgccattga actggctttg ccgcactcaa caaggaaacg cgcaaaatga ccgcgcgtca
     9841 ctgcgccgcc ctgcgccagt cgttgtgcgc cttccagcgc cccaggtatt tgcgcttttt
     9901 caagtctttc ggcaatttgc tgcgcccgct gattgcgccg ttctgtctgc tgcgcgagga
     9961 actcacacat tagcggatga gtaatatcaa tattcagccc gacaatatga atttcatgat
    10021 tttcccagac cgtggaaatt tccacgccgg gaataagatt cagcgccagt cccgaacgtg
    10081 aaatttcctc tcttgctggc gcgattgcag ctgtggtgtc atggtcggtg atcgccaggg
    10141 tgccgacgcg catctcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg
    10201 aagctgtggt atggctgtgc aggtcgtaaa tcactgcgta attcgtgtcg ctcaaggcgc
    10261 actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg
    10321 aaatcagctg ttgacaatta atcatcgaac tagctaacta gtacgcaagt tcacgtaaaa
    10381 agggtatcga caatgaaagc aattttcgta ctgaaagctt ggtggcgcac ttcctgaaac
    10441 gggcagtgta ttcaccatgc gtaaagcaat cagataccca gcccgcctaa tgagcgggct
    10501 tttttttgaa caaaattaga gaataacaat gcaaacacaa aaaccgactc tcgaactgct
    10561 aacctgcgaa ggcgcttatc gcgacaaccc gactgcgctt tttcaccagt tgtgtgggga
    10621 tcgtccggca acgctgctgc tggaatccgc agatatcgac agcaaagatg atttaaaaag
    10681 cctgctactg gtagacagtg cgctgcgcat tacagcatta ggtgacactg tcacaattca
    10741 ggcactttcc ggcaacggcg aagccctgct gacactactg gataacgccc tgcctgcggg
    10801 tgtggaaaat gaacaatcac caaactgccg tgtgctgcgc ttcccccctg tcagtccact
    10861 gctggatgaa gacgctcgct tatgctccct ttcggttttt gacgctttcc gtttattgca
    10921 gaatctgttg aatgtaccga aggaagaacg agaagcaatg ttcttcggcg gcctgttctc
    10981 ttatgacctt gtggcgggat ttgaagattt accgcaactg tcagcggaaa ataactgccc
    11041 tgatttctgt ttttatctcg ctgaaacgct gatggtgatt gaccatcaga aaaaaaacac
    11101 ccgcattcag gccagcctgt ttgctccgaa tgaagaagaa aaacaacgtc tcactgctcg
    11161 cctgaacgat cttcgccagc agctgaccga agccgcgccg ccgctgccgg tggtttccgt
    11221 gccgcatatg cgttgtgaat gtaaccagag cgatgaagag ttcggtggcg tggtgcgttt
    11281 gttgcaaaaa gcgattcgca ccggagaaat tttccaggtg gtgccgtctc gccgtttctc
    11341 tctgccctgc ccgtcaccgc tggcggccta ttacgtgctg aaaaagagta atcccagccc
    11401 gtacatgttt tttatgcagg ataatgattt caccctgttt ggcgcgtcgc cggaaagttc
    11461 gctcaagtat gacgccacca gccgccagat tgagatctac ccgattgccg gaacacgccc
    11521 gcgcggtcgt cgcgccgatg gttcactgga cagagacctc gacagccgca tcgaactgga
    11581 aatgcgtacc gatcataaag agctttctga acatctgatg ctggtggatc tcgcccgtaa
    11641 tgatctggca cgcatttgca cccccggcag ccgctacgtc gccgatctta ccaaagttga
    11701 ccgttactct tacgtgatgc acctggtctc ccgcgtggtc ggtgagctgc gccacgatct
    11761 cgacgccctg cacgcttacc gcgcctgtat gaatatgggg acgttaagcg gtgcgccgaa
    11821 agtacgcgcc atgcagttaa ttgccgaggc ggaaggtcgt cgccgcggca gctacggcgg
    11881 cgcggtaggt tatttcaccg cgcatggcga tctcgacacc tgcattgtga tccgctcggc
    11941 gctggtggaa aacggtatcg ccaccgtgca agccggtgct ggcatagtcc ttgattctgt
    12001 tccgcagtcg gaagccgacg aaacccgtaa taaagcccgc gctgtactgc gcgctattgc
    12061 caccgcgcat catgcacagg agactttctg atggctgaca ttctgctgct cgataatatc
    12121 gactctttta cgtacaacct ggcagatcag ttgcgcagca atgggcataa tgtggtgatt
    12181 taccgcaacc atattccggc gcagacctta attgaacgcc tggcgacgat gagcaatccg
    12241 gtgctgatgc tttctcctgg ccccggtgtg ccgagcgaag ccggttgtat gccggaactc
    12301 ctcacccgct tgcgtggcaa gctgcccatt attggcattt gcctcggaca tcaggcgatt
    12361 gtcgaagctt acgggggcta tgtcggtcag gcgggcgaaa ttctccacgg taaagcctcc
    12421 agcattgaac acgacggtca ggcgatgttt gccggattaa caaacccgct gccagtggcg
    12481 cgttatcact cgctggttgg cagtaacatt ccggccggtt taaccatcaa cgcccatttt
    12541 aatggcatgg tgatggcagt acgtcacgat gcggatcgcg tttgtggatt ccagttccat
    12601 ccggaatcca ttcttactac ccagggcgct cgcctgctgg aacaaacgct ggcctgggcg
    12661 cagcggaaac tagagccaac caacacgctg caaccgattc tggaaaaact gtatcaggcg
    12721 cagacgctta gccagcaaga aagccaccag ctgttttcag cagtggtacg tggcgaactg
    12781 aaaccggaac aactggcggc ggcgctggtg agcatgaaaa ttcgcggcga gcacccgaac
    12841 gagatcgccg gagcagcaac cgcgttactg gaaaacgccg cgccgttccc gcgcccggat
    12901 tatctgtttg ccgatatcgt cggcaccggt ggtgacggca gtaacagcat caatatttcc
    12961 accgccagtg cgtttgtcgc cgcggcctgt gggctgaaag tggcgaaaca cggcaaccgt
    13021 agcgtctcca gtaaatctgg ttcgtccgat ctgctggcgg cgttcggtat taatcttgat
    13081 atgaatgccg ataaatcgcg ccaagcgctg gatgagttag gcgtatgttt cctctttgcg
    13141 ccgaaatatc acaccggatt tcgccatgcg atgccggttc gccagcaact gaaaacccgc
    13201 actctgttca acgtgctggg accattgatt aacccggcgc atccgccgct ggcgttaatt
    13261 ggtgtttata gtccggaact ggtgctgccg attgccgaaa ccttgcgcgt gctggggtat
    13321 caacgcgcgg cggtagtgca cagcggcggt atggatgaag tttcattaca cgcgccgaca
    13381 atcgttgccg aactgcatga cggtgaaatt aagagctatc agctaactgc agaagatttt
    13441 ggcctgacac cctaccacca ggagcaactg gcaggcggaa caccggaaga aaaccgtgac
    13501 attttaacac gcttgttaca aggtaaaggc gacgccgctc acgaagcagc cgtcgcggcg
    13561 aatgtcgcca tgttaatgcg cctgcatggc catgaagatc tgcaagccaa tgcgcaaacc
    13621 gttcttgagg tactgcgcag tggttccgct tacgacagag ttaccgcact ggcggcacga
    13681 gggtaaatga tgcaaaccgt tttagcgaaa atcgtcgcag acaaggcgat ttgggtagaa
    13741 acccgcaaac agcagcaacc gctggccagt tttcagaatg aggttcagcc gagcacgcga
    13801 catttttatg atgcgctaca gggtgcacgc acggcgttta ttctggagtg caagaaagcg
    13861 tcgccgtcaa aaggcgtgat ccgtgatgat ttcaatccgg cacgcattgc cgccatttat
    13921 aaacattacg cttcggcaat ttcggtgctg actgatgaga aatattttca ggggagcttt
    13981 gatttcctcc ccatcgtcag ccaaatcgcc ccgcagccga ttttatgtaa agacttcatt
    14041 atcgaccctt accagatcta tctggcgcgc tattaccagg ccgatgcctg cttattaatg
    14101 ctttcagtac tggatgacga ccaatatcgc cagcttgcag ccgtcgctca cagtctggag
    14161 atgggggggc tgaccgaagt cagtaatgaa gaggaactgg agcgcgccat tgcattaggg
    14221 gcaaaggtcg ttggcatcaa caaccgcgat ctgcgtgatt tgtcgattga tctcaaccgt
    14281 acccgcgagc ttgcgccgaa actggggcac aacgtaacgg taatcagcga atccggcatc
    14341 aatacttacg ctcaggtgcg cgagttaagc cacttcgcta acggttttct gattggttcg
    14401 gcgttgatgg cccatgacga tttgcacgcc gccgtgcgcc gggtgttgct gggtgagaat
    14461 aaagtatgtg gcctgacgcg tgggcaagat gctaaagcag cttatgacgc gggcgcgatt
    14521 tacggtgggt tgatttttgt cgcgacatca ccgcgttgcg tcaacgttga acaggcgcag
    14581 gaagtgatgg ctgcggcacc gttgcagtat gttggcgtgt tccgcaatca cgatattgcc
    14641 gatgtggtgg acaaagctaa ggtgttatcg ctggcggcag tgcaactgca tggtaatgaa
    14701 gatcagctgt atatcgatac gctgcgtgaa gctctgccaa cacatgttgc catctggaaa
    14761 gcattaagcg tcggtgaaac cctgcccgcc cgcgagtttc agcacgttga taaatatgtt
    14821 ttagacaacg gccagggtgg aagcgggcaa cgttttgact ggtcactatt aaatggtcaa
    14881 tcgcttggca acgttctgct ggcggggggc ttaggcgcag ataactgcgt ggaagcggca
    14941 caaaccggct gcgccggact tgattttaat tctgctgtag agtcgcaacc gggcatcaaa
    15001 gacgcacgtc ttttggcctc ggttttccag acgctgcgcg catattaagg aaaggaacaa
    15061 tgacaacatt acttaacccc tattttggtg agtttggcgg catgtacgtg ccacaaatcc
    15121 tgatgcctgc tctgcgccag ctggaagaag cttttgtcag cgcgcaaaaa gatcctgaat
    15181 ttcaggctca gttcaacgac ctgctgaaaa actatgccgg gcgtccaacc gcgctgacca
    15241 aatgccagaa cattacggct gggacgaaca ccacgctgta tctgaagcgc gaagatttgc
    15301 tgcacggcgg cgcgcataaa actaaccagg tgctcggtca ggctttactg gcgaagcgga
    15361 tgggtaaaac cgaaatcatc gccgaaaccg gtgccggtca gcatggcgtg gcgtcggccc
    15421 ttgccagcgc cctgctcggc ctgaaatgcc gtatttatat gggtgccaaa gacgttgaac
    15481 gccagtcgcc taacgttttt cgtatgcgct taatgggtgc ggaagtgatc ccggtacata
    15541 gcggttccgc gacgctgaaa gatgcctgta acgaggcgct gcgcgactgg tccggcagtt
    15601 atgaaaccgc gcactatatg ctgggcaccg cagctggccc acatccttat ccgaccattg
    15661 tgtgtgagtt tcagcggatg attggcgaag aaacgaaagc gcagattctg gaaagagaag
    15721 gtcgcctgcc ggatgccgtt atcgcctgtg ttggcggcgg ttcgaatgcc atcggcatgt
    15781 ttgcagattt catcaacgaa accaacgtcg gcctgattgg tgtggagccg ggtggtcacg
    15841 gtatcgaaac tggcgagcac ggcgcaccgc taaaacatgg tcgcgtgggc atttatttcg
    15901 gtatgaaagc gccgatgatg caaaccgaag acgggcagat tgaagaatct tactccatct
    15961 ccgccggact ggatttcccg tctgtcggcc cacaacacgc gtatcttaac agcactggac
    16021 gcgctgatta cgtgtctatt accgatgatg aagcccttga agccttcaaa acgctgtgcc
    16081 tgcacgaagg gattatcccg gcgctggagt cttcccacgc cctggcccat gcgctgaaaa
    16141 tgatgcgcga aaccccggaa aaagagcagc tactggtggt taacctttcc ggtcgcggcg
    16201 ataaagacat cttcaccgtt cacgatattt tgaaagcacg aggggaaatc tgatggaacg
    16261 ctacgaatct ctgtttgccc agttgaagga gcgcaaagaa ggcgcattcg ttcctttcgt
    16321 cacgctcagg gatccgggca ttgagcagtc attgaaaatt atcgatacgc taattgaagc
    16381 cggtgctgac gcgctggagt taggtatccc cttctccgac ccactggcgg atggcccgac
    16441 cattcaaaac gccactctgc gcgcctttgc ggcaggtgtg actccggcac aatgttttga
    16501 aatgctggca ctgattcgcc agaaacaccc gaccattccc attggcctgt tgatgtatgc
    16561 caatctggtg tttaacaaag gcattgatga gttttatgcc cagtgcgaga aagtcggcgt
    16621 cgattcggtg ctggttgccg atgtgccagt tgaagagtcc gcgcccttcc gccaggccgc
    16681 gttgcgccat aatgtcgcac ctatcttcat ctgcccgcca aatgccgatg acgacctgct
    16741 gcgccagata gcctcttacg gtcgtggtta cacctatttg ctgtcacgag cgggcgtgac
    16801 cggcgcagaa aaccgcgccg cgttacccct caatcatctg gttgcgaagc tgaaagagta
    16861 caacgctgca cctccattgc aggggtttgg tatttccgcc ccggatcagg taaaagcagc
    16921 gattgatgca ggagctgcgg gcgcgatttc tggttcggcc attgttaaaa tcatcgagca
    16981 acatattaat gagccagaga aaatgctggc ggcactgaaa gtttttgtac aaccgatgaa
    17041 agcggcgacg cgcagttaat cccacagccg ccagttccgt tggcggcatt ttaactttct
    17101 ttaatgaagc cggaa
//
LOCUS       DAJTYD010000074        16816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTYD010000074 DAJTYD010000000
VERSION     DAJTYD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.074"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            260..1078
                     /gene="ybhA"
                     /locus_tag="OO585_002215"
     CDS             260..1078
                     /gene="ybhA"
                     /locus_tag="OO585_002215"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6629795.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1079..2137)
                     /gene="modC"
                     /locus_tag="OO585_002216"
     CDS             complement(1079..2137)
                     /gene="modC"
                     /locus_tag="OO585_002216"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6629796.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2140..2829)
                     /gene="modB"
                     /locus_tag="OO585_002217"
     CDS             complement(2140..2829)
                     /gene="modB"
                     /locus_tag="OO585_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6629797.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2829..3602)
                     /gene="modA"
                     /locus_tag="OO585_002218"
     CDS             complement(2829..3602)
                     /gene="modA"
                     /locus_tag="OO585_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6629798.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3768..3917)
                     /gene="acrZ"
                     /locus_tag="OO585_002219"
     CDS             complement(3768..3917)
                     /gene="acrZ"
                     /locus_tag="OO585_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6629799.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4046..4834
                     /gene="modE"
                     /locus_tag="OO585_002220"
     CDS             4046..4834
                     /gene="modE"
                     /locus_tag="OO585_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6629800.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4902..6374
                     /gene="modF"
                     /locus_tag="OO585_002221"
     CDS             4902..6374
                     /gene="modF"
                     /locus_tag="OO585_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6629801.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6635..7651
                     /gene="galE"
                     /locus_tag="OO585_002222"
     CDS             6635..7651
                     /gene="galE"
                     /locus_tag="OO585_002222"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6629802.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7661..8707
                     /gene="galT"
                     /locus_tag="OO585_002223"
     CDS             7661..8707
                     /gene="galT"
                     /locus_tag="OO585_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6629803.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8711..9859
                     /gene="galK"
                     /locus_tag="OO585_002224"
     CDS             8711..9859
                     /gene="galK"
                     /locus_tag="OO585_002224"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6629804.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9853..10893
                     /gene="galM"
                     /locus_tag="OO585_002225"
     CDS             9853..10893
                     /gene="galM"
                     /locus_tag="OO585_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6629805.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11095..11847
                     /gene="gpmA"
                     /locus_tag="OO585_002226"
     CDS             11095..11847
                     /gene="gpmA"
                     /locus_tag="OO585_002226"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6629806.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12005..13057)
                     /gene="aroG"
                     /locus_tag="OO585_002227"
     CDS             complement(12005..13057)
                     /gene="aroG"
                     /locus_tag="OO585_002227"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6629807.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13373..13753
                     /locus_tag="OO585_002228"
     CDS             13373..13753
                     /locus_tag="OO585_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6629808.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13867..14808
                     /gene="zitB"
                     /locus_tag="OO585_002229"
     CDS             13867..14808
                     /gene="zitB"
                     /locus_tag="OO585_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6629809.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14805..15524)
                     /gene="pnuC"
                     /locus_tag="OO585_002230"
     CDS             complement(14805..15524)
                     /gene="pnuC"
                     /locus_tag="OO585_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6629810.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(15562..16605)
                     /gene="nadA"
                     /locus_tag="OO585_002231"
     CDS             complement(15562..16605)
                     /gene="nadA"
                     /locus_tag="OO585_002231"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6629811.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4228 a         4401 c         4207 g         3980 t
ORIGIN      
        1 gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaaca ccacttgtaa
       61 ttaattgatt ataaaggatt aattcctaag aggcatttgc ttcatgaatg ctcctttgca
      121 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
      181 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
      241 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
      301 attgaccccg aaaaagaccc tgcttccttc atcgatagaa gccctggccc gcgctcgcga
      361 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
      421 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
      481 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
      541 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtggatgatg caatggtcta
      601 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
      661 acagcgtccg actttcacac aagtcgcttc tctggctgaa acggcgcaac aagttaacgc
      721 cgtatggaag ttcgccctga cgcacgatga catgccgcaa ttgcagcatt ttggtaagca
      781 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
      841 acgcggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggctggtc
      901 gatggaaaac gtcgtggcgt tcggcgataa ctttaatgat atcagtatgc tggaggccgc
      961 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1021 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1081 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggcctggt ttgatcgcca
     1141 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1201 ccacttccac ctgaccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1261 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1321 tacgtagcgc agcttgcagc ggttcgtcca gcttattgac ccacaaatgc tgatcgccca
     1381 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1441 tactttgttg ctctttcggc agccacggat tcatcacgct actaccccag acttcttcca
     1501 gtgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     1561 ggatctcatc cagcgaatgg ctgacataca acatcgggat gttgatttcc cgcgtcagac
     1621 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     1681 ccagcaacag caattctggt gccgtcagca aagcccgccc aatcgctacg cgctgttttt
     1741 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     1801 ccagcttatc gaactgatcg accatgcttt tcgccatgcc gtagcgcaga ttgccacgca
     1861 ctttgtaatg cggaaacagc cgcgcatcct gaaaaacata gccaacgcga cgcttttctg
     1921 gcgtcaggca gatacctttt tcggcatcat ttagtacccg accattgagg acaatcctcc
     1981 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2041 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaata gtcaggcaat
     2101 ggttgcccaa cgtctgggaa aaattcagtt ccagcatgat tagcgccccg cccgttcacg
     2161 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2221 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2281 agagggaatg gttcgcgttt cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2341 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2401 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     2461 ttcgagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     2521 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     2581 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     2641 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     2701 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     2761 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     2821 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     2881 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     2941 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3001 ctgacaactg cgtcagaacc gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac
     3061 gccagcgcgc cacgaacatc ttccgccgga gccaatttcg gggagagcgt atcccatgcg
     3121 cccagttttt gcagtgcttc ttttgcataa atgccagcag ggacatgttc cggatcgcca
     3181 accgcgaggc gaccgccatt cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa
     3241 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctgg
     3301 cgcgaagccg tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3361 gagataaaca gatccgcagg cgctcccgct tcaatctgac gggccagagt agatgacgaa
     3421 gcgaaagaag aaaccacatc cacgcctttt tcttttttat actgcgtagc aatgtcctgc
     3481 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgta
     3541 ttgccagcaa cagcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     3601 attggtaacc ccttaatgta acgttatata ggtgacaata taacggctaa ctcagaattt
     3661 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc
     3721 aggaagttga gaaaaagaaa tgcccgacta agtgggcatt cagggaatca atgattttgt
     3781 ccgggctggt cttttttacc aacaccagaa aagatgttga atacttcacc aagaccgtaa
     3841 atcagaccca ggatgatggc catcacgaca ggtaccatga ttacggcgaa taccagactt
     3901 tttaataact ctaacatggt caactccaga tatagtcatg agactattct aaccgctaag
     3961 cacagaaaag cactcccctt ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca
     4021 ataagacttt tgccaggaca ttgttatgca ggccgaaatc cttctcaccc ttaagctcca
     4081 acaaaaatta ttcgccgacc cgcgccggat ttcgctacta aaacacattg cgctttccgg
     4141 ttccattagc cagggagcga aagatgccgg tatcagctat aaaagcgcct gggatgccat
     4201 taacgagatg aatcagttaa gcgagcatat tctggttgag cgcgcaacag gcggtaaagg
     4261 tggcggcggc gcagtactga cccgctatgg tcagcgactg attcagctct atgacttact
     4321 ggcgcaaatc cagcaaaaag cctttgatgt gttaagtgac gatgacgccc tgccgctgaa
     4381 cagcctgctg gccgcgatct cacgtttttc actgcaaacc agcgcccgta accagtggtt
     4441 cggtaccatc accgcccgcg atcatgatga cgttcaacag catgttgatg tcttactggc
     4501 tgacggaaaa acacgcctga aagtcgcaat taccgcacaa agcggcgcac gtctggggct
     4561 ggatgaaggc aaagaagtgt tgatattgct aaaagcgccg tgggtaggca ttactcagga
     4621 cgaagcggtc gcacaaaacg ccaacaacca attaccgggt attattagtc atattgagcg
     4681 cggcgcagag cagtgcgaag tattaatggc gctacccgac gggcaaacac tgtgcgccac
     4741 agtgccggta aatgaagcga cttctcttca gcaagggcag aatgtcacgg cctactttaa
     4801 tgccgacagc gtgattatcg ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca
     4861 cgtatccctg tcagtaatcg ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt
     4921 tgcaaggcac gtttcgtctt agcgacacaa aaacgctgca attgcctcag ctaacgttaa
     4981 acgcgggtga tagttgggcg tttgtcggtt cgaatggaag cgggaaatcg gccctggccc
     5041 gcgcgctggc gggggaactt ccgcttttga aaggtgaacg gcaaagccag ttttcccaca
     5101 tcactcgtct ctccttcgag caattgcaaa aactcgtcag cgacgaatgg cagcggaata
     5161 acaccgatat gctcggccct ggcgaagatg acaccggacg cactacggct gagatcattc
     5221 aggatgaagt aaaggatgca ccgcgttgca tgcaactggc gcagcagttc ggtattaccg
     5281 ccctcctcga ccgacgcttt aaataccttt ccactggcga gacgcgaaaa atcctgctgt
     5341 gtcaggcgct gatgtcggag cctgacttgt tgattcttga tgagccgttc gatggcctgg
     5401 atgttgcctc acgtcagcag ctggctgagc gactcgcctc gttacatcag tccggtatta
     5461 ctctggtact ggtgctcaat cgcttcgatg agatcccgga gtttgtccag tttgctggcg
     5521 tgctggcgga ttgcacgtta gcggaaactg gcgctaaaga ggaactgctc caacaagcac
     5581 tcgtcgcgca actggcacat agtgaacagc ttgaaggtgt gcaactgccg gagccggatg
     5641 aaccttcagc acgtcacgcc ttacccgcca acgaaccgcg cattgtgctg aacaatggcg
     5701 tggtttctta taacgatcgc cccattctta ataaccttag ctggcaggtg aatccaggtg
     5761 aacactggca aattgtcggg ccaaatggtg caggaaaatc gacgttatta agcctggtta
     5821 ctggcgatca tccgcaaggt tacagcaacg atttgacgct tttcggacga cgtcgcggca
     5881 gcggcgaaac catctgggat atcaaaaagc atatcggtta cgtcagcagt agtttgcatc
     5941 tggattaccg ggtcagcact accgtgcgta atgtgattct ttctggctat tttgattcga
     6001 ttggcattta tcaggccgtt tcggatcgcc agcaaaaact ggtgcagcag tggctggata
     6061 ttctcggcat tgataaacgt acggctgacg ctccgttcca tagtctttcc tggggacagc
     6121 agcatctggc gctgatcgtc cgcgcactgg tgaaacatcc gacgttgctt attctcgatg
     6181 aaccactaca ggggcttgat ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc
     6241 tgattagcga aggtgaaacg caattgttgt ttgtttcgca ccacgctgaa gatgcgcctg
     6301 cctgtattac ccatcgcctt gagttcgtgc cggacggtgg actctatcgc tatgtgctga
     6361 caaaaatata ttgagtcggt agtgctgacc ttgccggagg cggcctaagc gccctctccg
     6421 gccaacggtt cgacgcatgc aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat
     6481 cattcataaa ccctctgttt tataatcact taatcgcgca taaaaaacgg ctaaattctt
     6541 gtgtaaacga ttccactaat ttattccatg tcacactttt cgcatctttg ttatgctatg
     6601 gttatttcat accataagcc taatggagcg aattatgaga gttctgctta ccggtggtag
     6661 cggttacatt ggaagtcata cctgcgtgca attactgcaa aacggtcatg atgtcatcat
     6721 tcttgataac ctctgtaaca gtaagcgcag cgtactgcct gttatcgagc gtttaggcgg
     6781 caaacatcca acgtttgttg aaggcgatat tcgtaacgaa gcgttgatga ccgagatcct
     6841 gcacgatcac gctatcgaca ccgtgatcca cttcgccggg ctgaaagccg ttggcgaatc
     6901 ggtacaaaaa ccgctggaat attacgacaa caatgtcaac ggtactctgc gcctgattag
     6961 cgccatgcgc gccgctaacg tcaaaaactt tatttttagc tcctccgcca ccgtttatgg
     7021 cgatcagccc aaaattccat acgttgaaag cttcccgacc ggcacaccgc aaagccctta
     7081 cggcaaaagc aagctgatgg tggaacagat cctcaccgat ctgcaaaaag cccagccgga
     7141 ctggagcatt gccctgctgc gctacttcaa cccggttggc gcgcatccgt cgggcgatat
     7201 gggcgaagat ccgcaaggca ttccgaataa cctgatgcca tacatcgccc aggttgctgt
     7261 aggccgtcgc gactcgctgg cgatttttgg taacgattat ccgaccgaag atggtactgg
     7321 cgtacgcgat tacatccacg taatggatct ggcggacggt cacgtcgtgg cgatggaaaa
     7381 actggcgaac aagccaggcg tacacatcta caacctcggc gctggcatag gcagcagcgt
     7441 gctggacgtg gttaatgcct tcagcaaagc ctgcggcaaa ccggttaact atcattttgc
     7501 accgcgtcgc gagggcgacc ttccggccta ctgggcggac gccagcaaag ccgaccgtga
     7561 actgaactgg cgcgtaacgc gcacactcga tgaaatggcg caggacacct ggcactggca
     7621 gtcacgccat ccacagggat atcccgatta aggaacgacc atgacgcaat ttaatcccgt
     7681 tgatcatcca catcgccgct acaacccgct caccgggcaa tggattctgg tttcaccgca
     7741 ccgcgctaag cgcccctggc agggggcgca ggaaacgcca gccaaacagg tgttacctgc
     7801 gcacgatcca gattgcttcc tctgcgcagg taatgtgcgg gtgacaggcg ataaaaacca
     7861 cgattacacc gggacttacg ttttcactaa tgactttgcg gccttgatgt ctgacacgcc
     7921 agatgcgcca gaaagcaacg atcctttgat gcgttgccag agcgcgcgcg gtaccagccg
     7981 ggtgatctgc ttttcaccgg atcacagtaa aacgttgcca gaactgagcg ttgcggcatt
     8041 gacggaaatc gtcaaaacct ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg
     8101 ggtgcaggtc tttgaaaaca aaggtgcggc gatgggctgc tctaacccgc atccgcacgg
     8161 acagatttgg gcaaatagct tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa
     8221 agaatatttt gccgaacaga aatcaccaat gctggtggat tatgttcagc gcgagctggc
     8281 agacggtagc cgtaccgttg tcgaaaccga acactggtta gccgttgtgc cttactgggc
     8341 tgcctggccg ttcgaaacgc tactgctgcc caaagcccac gttttgcgga tcaccgattt
     8401 gaccgacgcc cagcgcagcg atttggcgct ggcgttgaaa aagctgacca gtcgttatga
     8461 caacctcttc cagtgctcct tcccctactc tatgggctgg cacggcgcac catttaatgg
     8521 cgaagagaat caacactggc agctgcacgc gcacttttat ccgcctctgc tgcgctccgc
     8581 caccgtacgt aaatttatgg ttggttatga aatgctggca gagacccagc gagacctgac
     8641 cgcagaacag gcagcagagc gtttgcgcgc agtcagcgat atccattttc gcgaatccgg
     8701 agtgtaagaa atgagtctga aagaaaaaac acaatctctg tttgccaacg catttggcta
     8761 ccctgccact cacaccattc aggcgcctgg ccgcgtgaat ttgattggtg aacacacgga
     8821 ctacaacgac ggtttcgttc tgccctgcgc gattgattat caaaccgtga tcagctgtgc
     8881 accacgcgat gaccgtaaag ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga
     8941 gttttccctc aatgcgccca ttgtcgcgca tgaaaactat caatgggcga actacgttcg
     9001 tggcgtggtg aaacatctgc aactgcgtaa caacagcttc ggcggtgtgg acatggtgat
     9061 cagcggcaat gtgccgcagg gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt
     9121 cggaaccgta ttgcagcagc tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa
     9181 cggtcaggaa gcagaaaacc agtttgttgg ctgtaactgc gggatcatgg atcagctaat
     9241 ttccgcactc ggcaagaaag atcatgcctt gctgattgac tgtcgttcac tggggaccaa
     9301 agcagtttcc atgccgaaag gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac
     9361 cctggttggc agcgaataca acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt
     9421 ccagcagcca gccctgcgcg atgtcaccat tgaagagttc aacgctgttg cacatgagct
     9481 ggacccaatc gtggcgaaac gcgtgcggca tatcctgact gaaaacgccc gcaccgttga
     9541 agctgccagc gcgctggagc agggcgacct gaaacgtatg ggcgagttga tggcggagtc
     9601 tcatgcctct atgcgcgatg atttcgaaat caccgtgccg caaattgaca ctctggtaga
     9661 aatcgtcaaa gctgtgattg gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg
     9721 cggctgtatc gtcgcgttga tcccggaaga gctggtgcct gccgtacagc aagctgtcgc
     9781 tgaacaatat gaagcaaaaa caggtattaa agagactttt tacgtttgta aaccatcaca
     9841 aggagcagga cagtgctgaa cgaaactccc gcactggcac ccgatggtca gccgtaccga
     9901 ctgttaactt tgcgtaacaa cgcagggatg gtagtcacgc tgatggactg gggtgcgact
     9961 ttactttcag cccgtattcc gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt
    10021 gccagcccgg aatgctatca ggatcaggcc gcgtttctgg gggcctctat tggtcgttat
    10081 gccaaccgca tcgccaatag ccgttatacc tttgacggtg aaaccgtgac cctttcgcca
    10141 agtcagggcg ttaaccagct gcacggcggc ccggaagggt tcgacaaacg tcgctggcag
    10201 attgtgaacc agaacgatcg tcaggtgctg tttgccctga gttcagatga tggcgatcag
    10261 ggcttcccgg gtaatctcgg cgcaacggtg caatatcgtc tgaccgacga taaccgtatc
    10321 tccattactt atcgcgccac agttgataaa ccttgcccgg tgaatatgac taatcacgtc
    10381 tatttcaatc ttgacggcga gcagtctgac gtgcgcaatc acaagttgca gattctggcg
    10441 gacgaatatc tgccggttga tgaaggcggc attccgcacg acggcctgaa atctgtcgcc
    10501 ggaacgtctt ttgatttccg cagcgccaaa atcatcgcca gtgagtttct tgccgacgac
    10561 gatcagcgca aagtgaaagg ttacgatcac gcattcttgt tacaggccaa aggcgatggc
    10621 aagaaagtgg cggcgcatgt ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc
    10681 accgctccgg ctctgcaatt ctactccggc aacttcctcg gcggcacacc gtcgcgggga
    10741 acagaacctt acgccgactg gcaagggctg gcgttggaaa gcgagtttct gccggacagc
    10801 ccgaaccacc ctgaatggcc gcaaccggac tgcttcctgc gtcctggcga agagtattcc
    10861 agcctgacgg aatatcagtt tattgctgag taatgttaag ccctcgtcat gagggcttta
    10921 tctcatattg ctcaaatcac cagcaaacac cgacatattt gcaactcaat attcacaaca
    10981 accttacact gcgccactat tttcgctatg gttatgcgta agcattgctg ttgcttcgtc
    11041 gcggcaatat aatgagaatt attatcatta aaagatgatt tgaggagtaa gtatatggct
    11101 gtaactaagc tggttctggt tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc
    11161 accggttggt acgacgtgga tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt
    11221 aagctgctga aagaggaagg ttacagcttt gactttgctt acacttctgt gctgaaacgc
    11281 gctatccata ccctgtggaa tgtgctggac gaactggatc aggcatggct gcccgttgag
    11341 aaatcctgga aactgaacga acgtcactac ggtgcgttgc agggtctgaa caaagcggaa
    11401 actgctgaaa agtatggcga cgagcaggtg aaacagtggc gtcgtggttt tgcagtgact
    11461 ccgccggaac tgactaaaga tgatgagcgt tatccgggtc acgatccgcg ttacgcgaaa
    11521 ctgagcgaga aagaactgcc gctgacggaa agcctggcgc tgaccattga ccgcgtgatc
    11581 ccttactgga atgaaactat tctaccgcgt atgaagagcg gtgagcgcgt aatcatcgcc
    11641 gcacacggta actccctgcg tgcgctggtg aaatatcttg ataacatgag cgaagaagag
    11701 attcttgagc tgaatatccc gactggcgtg ccgctggtgt atgagttcga cgagaatttc
    11761 aaaccgctga aacgctatta tctgggtaat gctgacgaga tcgcagcgaa agcagcggcg
    11821 gttgcaaacc agggtaaagc gaagtaaacg tcattcgttt aaaatgagaa agccgactgc
    11881 aagtgagtcg gcttttttgt tgctaacaat ggagcacatt gcctgatgcg acgcttcgcg
    11941 acttatcagg cctgtggtga ttcatcggat acgccactct gacggcgcat ccgacaatta
    12001 aaccttaccc gcgacgcgct tttactgcat tcgccagttg acgtaacaga gcatcggtat
    12061 cttcccagcc gatgcaggca tcggtgatgc tcttaccgta ggccagcggc tccccgctct
    12121 cgaggctctg attgccttcc accagatggc tttccaccat cacgccaata atggcctttt
    12181 cgccaccggc aatctgctgg caaacgtcag cacaaacatc catctgcttt ttgaattgtt
    12241 tggacgagtt agcatggctg aaatcgatca tcacctgtgc tggcaggcct gctttgttca
    12301 gcccttcttt cacttcagca acgtgcttcg cgctgtagtt aggctcttta ccgccgcgca
    12361 gaatgatatg gcaatcgccg ttaccgctgg tattcacaat cgccgaatgc ccccatttcg
    12421 ttacggacag gaagcagtgc ggcgcaccgg cggcattaat ggcatcgata gccactttaa
    12481 tcgtaccgtc ggtgccattt ttgaagccga ctggacaaga aagccctgat gccagttcgc
    12541 ggtgcacctg cgattcggtg gtacgtgcgc caattgcgcc ccagctcatc aggtcagcga
    12601 gatattgtgg ggtgatcata tcgagaaact cacccgccgc tggcagaccg ctgtcgttaa
    12661 tatcaagcag caatttacgg gctatacgaa gaccgtcgtt gatctggaag ctgttatcca
    12721 tatgcggatc gttaatcagc cctttccagc ccaccgtggt acgcggcttt tcaaaataga
    12781 cgcgcattac gatttccagc tcatctttca gctcttcacg cagcgccagc aagcgagtgg
    12841 catactcttt agccgcgaca ggatcatgaa ttgagcacgg gccaatcacc accaacaggc
    12901 gatcatcatt acctttcagg atcttatgga tcgcttttcg ggcatgggca accgtattcg
    12961 cggcattttc agtagcgggg aatttttcca gcaatgcgac aggaggaagt aactctttga
    13021 tttctttgat gcgtaaatcg tcgttctgat aattcatgtc tgttccagtg ttgccatact
    13081 tatcttagtg aatgcaatac ttccaatcta tatcttccgt cagaatgtgt aaacggggtt
    13141 ttacattatg aacggattaa tcctggaatt agcaagaaaa acgccagatt gtcgcgaaaa
    13201 acgagatctc tcctacaatt tctaactgta actcctttgt ttgttaatta tttcaagatt
    13261 ctctgctgcg tttcataaac tggctgaaat cttaaaccaa tgccttatat tcacctgcaa
    13321 atgcactgtt ggaagaggtt atccgacata acgaccataa caggagcatc ctatgaaaat
    13381 gacaaaactg gccacattat ttctgactgc cactctaagc cttgccagcg gtgccgcact
    13441 ggctgccgat agcggagcgc aaactaataa cggccaggca aacgccgcaa ctgatgcggg
    13501 ccaggtagcc cctgacgccc gtgaaaatgt cgcgccaaat aacgtcgaca ataacggggt
    13561 aaataccggt tctggcggca caatgctgca ttcggatggt tcttcaatga acaatgacgg
    13621 aatgaccaaa gatgaagagc acaagaacac catgtgcaaa gatggtcgat gtccggatat
    13681 caataaaaag gtacaaaccg gtgatggcat caataatgat gtcgatacca aaaccgacgg
    13741 taccacgcag taaccctgca aaatagccaa taatgggaga gcggagtctc tcccttttaa
    13801 tttttcactt ctaaaagcag tatgatgtca taaagttaag tgagaataat taagggatga
    13861 ctcgtcatgg cgcactcaca ctcacacacg tcctcacacc tgccagaaga taataatgct
    13921 cgtcgcttgt tgtatgcttt cggcgtgact gccgggttta tgctggtaga agtcgttggt
    13981 ggttttcttt ctggttctct ggcattgctg gccgatgcgg gtcatatgtt gaccgatact
    14041 gccgccctgc ttttcgccct actcgccgtg caattttcac gtcgtcctcc caccattcgc
    14101 cacactttcg gttggctaag actgaccacg ctcgcggcgt ttgtgaatgc catcgccctc
    14161 gtggtaatta ccattttgat tgtctgggag gcgatagaac gcttccgcac gccgcgtccg
    14221 gtcgagggcg gcatgatgat ggcaattgcc gtggctgggt tgctggcaaa tatactttct
    14281 ttctggttac ttcatcacgg cagcgaagag aaaaacctca acgtacgagc agcggcattg
    14341 catgtgctcg gggacctgtt gggttcggtt ggggctatta ttgccgcgtt gattattatc
    14401 tggaccggct ggacacctgc tgaccccatt ctctcgatag tggtgtcgct tctggttctg
    14461 cgtagcgcgt ggcgactgtt gaaagatagc gtgaatgaat tacttgaagg tgcaccggta
    14521 tcgctggata tcgctgaact gaagcgtcgt atgtgccggg aaatcccgga agtccgcaat
    14581 gtgcaccatg tacatgtatg gatggtaggt gagaagccgg tgatgacgct gcatgtgcag
    14641 gtgatcccgc cacacgctca cgatgccttg ttggatcaga tccaacacta cctgatggat
    14701 cactatcaga ttgagcacgc caccattcag atggaatatc aaccttgtca tgggccagac
    14761 tgccacctga atgaaggggt gtctggccat tcacatcatc accattaatg ggacagcgcg
    14821 catgagcctc tttcacgtgc gctgttgatc cacatccgac tgccgttgag cgcaataaag
    14881 gtcaggatga tgtactccag agacatggcg taaacgccct gaagtgcaaa aataacgacg
    14941 ctaatcacgt taataatcac ccacaacagc cagttttcca catacttacg cgtcatcaga
    15001 agcattgcca cgatagataa caccatcatg catgaatccc agaacgggaa agcgtccggt
    15061 tgcagttcag gcatcacaac ctgtaatcct aatgcttgca tgatcatgac tgccacgcgg
    15121 gtcaaaaatg caaacaccgg attgataaat accgtcatca gaccaatcga aacaacgcaa
    15181 accgccaacc agctgagcgc cttcggcaat ggtaaccagc gaattttcaa ctccgcctcg
    15241 ttctgactgg tttgtcgcga ccacgcatac caaccgtaaa tattcgcggc aaagaaaaac
    15301 acctgtaata gcaggctggc atacagctga atctgaaaga aaataatgcc aaacaaggtg
    15361 acgttgatca ggccaaagaa gtagttgctg atcttctcca gactggcaag gccaatacac
    15421 agcaacccgg cgatcgtgcc taccgcttcg atccatgaga gatcataacc gcctgctcct
    15481 attggtatat gtaccaggat attctgcaca ctaaaaaaat ccatctttcc cccaaagcgt
    15541 aacgttacgc cttattattc gttatccacg tagtgtagcc gcaaaatcca gcatacgatt
    15601 gagcggcacc agcgccctct ctcgcagcct ttcatcaaca tgaacctcgt gattgcttcc
    15661 ttcctgttct aatgcctctg cgatggcctg aaggccattc atggccatcc acggacaatg
    15721 cgcgcagctg cggcaggttg caccctcacc tgcggttggt gcttccagta actctttatc
    15781 tggcaccgcc tgctgcattt tgtagaaaat accccgatcg gttgccacaa taagcctctg
    15841 atgtggcaat gttttcgcag cagcgatcag ttgactggtg gaaccgaccg catccgccat
    15901 atcgacaata gcttgtggtg attctggatg caccagtatg gcagcatccg ggtattcttc
    15961 ttgcaagcgg gttaacgcct gagtcttaaa ttcatcatgc acaatacagg caccctgcca
    16021 gcatagaatg tctccacccg tctgtttttg cacgtaacgc cccagatgtt tgtcgggtgc
    16081 ccagatgatt ttttcaccca aactatcaag atgatcaata agttcgacgg caatgcttga
    16141 agttaccacc caatctgcgc gcgcttttac cgcagcagaa gtgttggcgt agacgacgac
    16201 agtacgatcg ggatgggcat cgcaaaatgc gttaaattct tcaacagggc agccgagatc
    16261 cagtgaacat tcagcctgaa gtgtcggcat cagaattgtt ttttccggac tgagaatttt
    16321 ggcggtttct cccataaatc tcaccccagc gactaacaaa gtagaagcgg gatgctttgc
    16381 accgaagcgc gccatttcca gagaatcaga aatacagcca ccggtttctt ctgccagttg
    16441 ttgaatttcg ggatcggtat agtagtgggc aaccatcacc gcattacgtt cttttagcag
    16501 acgttttatc ttctcgcggt aatacgcttt ttcatcaatg cttaacggcg tcggcttcgg
    16561 ggggaaagga taaatcgccg tgtctggatc aaacattacg ctcatcttac catctcgttt
    16621 tacaggctta acgttaaaac cgacattagc gtaatattcg ctgttatcgg tgtaatttgt
    16681 tttatatgct aaacaagata gtcgaatggt ggggaaaagt cactcgaatt acgcctaatt
    16741 ccaccgacgt agagttgctt gcacagtaag ctctgacgat gtcatcaggt ttacaaagtg
    16801 aaattgagga tgcctg
//
LOCUS       DAJTYD010000075        16806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTYD010000075 DAJTYD010000000
VERSION     DAJTYD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.075"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            77..514
                     /locus_tag="OO585_002232"
     CDS             77..514
                     /locus_tag="OO585_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6629812.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            648..1192
                     /gene="cobU"
                     /locus_tag="OO585_002233"
                     /pseudo
     CDS             648..1192
                     /gene="cobU"
                     /locus_tag="OO585_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            1189..1932
                     /gene="cobS"
                     /locus_tag="OO585_002234"
     CDS             1189..1932
                     /gene="cobS"
                     /locus_tag="OO585_002234"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6629813.1"
                     /translation="MSKLFWTMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            1944..3023
                     /gene="cobT"
                     /locus_tag="OO585_002235"
     CDS             1944..3023
                     /gene="cobT"
                     /locus_tag="OO585_002235"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6629814.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            3085..4020
                     /gene="ldtA"
                     /locus_tag="OO585_002236"
     CDS             3085..4020
                     /gene="ldtA"
                     /locus_tag="OO585_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6629815.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(4076..4151)
                     /locus_tag="OO585_002237"
     tRNA            complement(4076..4151)
                     /locus_tag="OO585_002237"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4116..4118),aa:Asn,seq:gtt)
     gene            4478..4849
                     /locus_tag="OO585_002238"
                     /pseudo
     CDS             4478..4849
                     /locus_tag="OO585_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4848..5282
                     /locus_tag="OO585_002239"
                     /pseudo
     CDS             4848..5282
                     /locus_tag="OO585_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            5625..6341
                     /locus_tag="OO585_002240"
     CDS             5625..6341
                     /locus_tag="OO585_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6629816.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6797..6872)
                     /locus_tag="OO585_002241"
     tRNA            complement(6797..6872)
                     /locus_tag="OO585_002241"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6837..6839),aa:Asn,seq:gtt)
     gene            complement(6973..8616)
                     /gene="yeeO"
                     /locus_tag="OO585_002242"
     CDS             complement(6973..8616)
                     /gene="yeeO"
                     /locus_tag="OO585_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6629817.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            8621..8696
                     /locus_tag="OO585_002243"
     tRNA            8621..8696
                     /locus_tag="OO585_002243"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8654..8656,aa:Asn,seq:gtt)
     gene            8858..10120
                     /locus_tag="OO585_002244"
     CDS             8858..10120
                     /locus_tag="OO585_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6629818.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(10832..11716)
                     /locus_tag="OO585_002245"
     CDS             complement(10832..11716)
                     /locus_tag="OO585_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCR6629819.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRYVLETARAKDPGMAAALELSRLMGLRSQEVVQSAQSLR
                     TWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPD
                     LKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGH
                     GDGRGRYVAQVYGRKDTD"
     gene            12275..12421
                     /locus_tag="OO585_002246"
     CDS             12275..12421
                     /locus_tag="OO585_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629820.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(12581..12898)
                     /locus_tag="OO585_002247"
     CDS             complement(12581..12898)
                     /locus_tag="OO585_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6629821.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(12899..13156)
                     /locus_tag="OO585_002248"
     CDS             complement(12899..13156)
                     /locus_tag="OO585_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6629822.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(13505..13711)
                     /locus_tag="OO585_002249"
     CDS             complement(13505..13711)
                     /locus_tag="OO585_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6629823.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(13708..14169)
                     /locus_tag="OO585_002250"
     CDS             complement(13708..14169)
                     /locus_tag="OO585_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629824.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(14540..16144)
                     /locus_tag="OO585_002251"
     CDS             complement(14540..16144)
                     /locus_tag="OO585_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629825.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(16157..16522)
                     /locus_tag="OO585_002252"
     CDS             complement(16157..16522)
                     /locus_tag="OO585_002252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629826.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
BASE COUNT         4431 a         3967 c         4036 g         4372 t
ORIGIN      
        1 tcaaggtgtg gcaatattgt ttcatggctt atcaaccaga gcgccattac aggaaattaa
       61 gtggagggcg agaggcatga aaacgataaa acccgaatcg atagctcttt tttgcctctt
      121 atctggagat gtggatctgg cgaaacatct ggctgatata ttgccaataa cctgttttgt
      181 tggtgaggac tggcagcaag atttccagcc tcttgatggt gggcctgtgc aatcgttatc
      241 agattcgtat agcgattatt cagtgctgct attgatagca gctgaaagcg agatatccgt
      301 accagaatgg ctcgctcatt ctgagggaac gacggtaatt ttgattgcag aacacaatgc
      361 ccgtttgctg actggtccgg ctggtggggc tgtagcatta atccattatc tgaatgaaat
      421 ctgtcattcc agagaaaccg ctgtcaatga cgcattttgt cggacgttaa cacgaggaag
      481 tctggctatt ccgcaatgcc atcttctttc gtaatggatt gagaatgctg gatttttaat
      541 tatattacgc gcagtattaa tcgcgtaaat gttttgcggt cagcaacggt gggggatcca
      601 ttgttctgtt caatgaatac catcatacaa ttgaatcagg agtcggtatg atgatttcgg
      661 tgacgggggg catacggagc gggaagagtc gccacgcaga ggcgcttatt ggggactctt
      721 cacaggttct gtatacgcca cctcgcaaat ccttgatgat gagatggctg cacggataga
      781 acatcatcgg caaggccgcc cgacgcactg gcacacagtg gagcgctggc aacatcttga
      841 tgaattaatt catgcagaca ttaacccgca tgaggctgta ttgctggaat gcgttaccac
      901 catggtgact aatctgttgt ttgattatgg cggcgataaa gaccctgatg aatgggatta
      961 tcaggcgatg gaacaggcga ttaatgctga gattcagtcg ttgattgctg cctgccaacg
     1021 ttgtcccgca aaggttgtat tagtgactaa cgaagtggga atggggattg tgccggagag
     1081 tcgtctggca cgacattttc gtgatattgc cgggcgggta aatcagcagc tggccgctgc
     1141 ggcaaatgaa gtatggttgg tggtttcggg tattggagta aaaatcaaat gagtaaatta
     1201 ttttggacga tgctctcttt tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga
     1261 ctggatttcg agcattattc tcgcggtatt attacttttc ctttaattgg attattgctt
     1321 ggcgcgatta gcgggctggt cttcatggtg ctgcaggcat ggtgtggcgt accactggcg
     1381 gcactgttta gcgtacttgt gctggcgctg atgaccgggg gattccacct ggacggcctc
     1441 gccgatacct gtgatggcgt attttctgca cgtagccgcg atcgcatgtt ggaaatcatg
     1501 cgtgatagcc gtttaggcac ccacggcggt ctggcattga tttttgttgt actggcaaag
     1561 attctggtat taagcgagtt ggctctgcgt ggcgaaccga tccttgcatt gctggcggca
     1621 gcatgtgcgg tcagccgcgg gactgccgca ttattgatgt atcgtcatcg ttatgcccgg
     1681 gaagaaggac ttggcaatgt gttcattggc aaaattgatg ggcgacaaac ctgcgtcaca
     1741 ctcggcttag ccgctatttt tgctgccgta ttattgccgg gtatgcatgg tgtggctgct
     1801 atggtggtga cgatggtggc gattttcatc ctcggtcagc ttctcaaacg tacgctgggc
     1861 gggcaaaccg gcgatacgct gggagctgct atagagcttg gtgaactggt cttcctgctg
     1921 gcactgctat aaagagacgt gacattcaaa cacttgccga tttactgaat acgatccctg
     1981 ctatcgatcc tgccgctatg tcgcgtgcac aacggcatat cgacgggtta ctcaaacctg
     2041 ttggtagcct gggaaggctg gaggcgcttg ccatacaact ggcaggaatg ccggggttga
     2101 atggcgttcc gcatgtgggc aaaaaagcgg tactggtcat gtgtgccgat cacggcgtct
     2161 gggaggaagg gggcgctatt tccccaaaag aagtgacagc cattcaggct gaaaatatga
     2221 cccgcggaac aaccggcgtg tgtgtgctgg cagcacaagc gggcgctaac gtctacgtaa
     2281 ttgatgttgg tattgatact gctgagccta tccccgggct tatcaacatg cgtgttgctc
     2341 gaggtagtgg caatattgct tcagctccgg caatgagtcg ccatcaggca gaaaagttgc
     2401 ttttggacgt catatgttat acgcgggagc tggcaaaaaa cggtgttacg ctgtttggtg
     2461 taggtgaact ggggatggca aacacgacac cggcagcggc aatagtcagc acaatcactg
     2521 gccgggctcc tgaagaagtg gttgggattg gcgcaaacct gccgacagat aaactggcta
     2581 ataaaattga tgttgtgcgc cgggcgatta cgttgaatca accaaatccg caggatggcg
     2641 ttgatgtcct ggcaaaagtg ggtggatttg atttggtcgg aatggctggc gtgatgttag
     2701 gcgctgcgtc ctgcggttta cccgtgttgc tggatggatt tctttcttat gctgctgcgc
     2761 tcgcagcctg tcagatgtct cctgcaatca aaccgtatct cattccttct cacctgtcgg
     2821 cagaaaaagg agcgcgtata gcgctgtcgc atttggggct ggagccttat ctcaatatgg
     2881 agatgcgttt aggtgagggg agtggcgcag ctctggcgat gcccatcatc gaagctgcct
     2941 gtgcgatata taacaacatg ggcgaacttg ctgccagtaa tattgttcta ccggggaata
     3001 cgacttctga tttgaacagt taaaaacgaa aagtctgata aaagctatac ttaacggata
     3061 gctttcgcga cttaggaaag ggacatgatg cgtcgtgtaa atattttttg ctcatttgct
     3121 ctgctttttg ccagccatac tagcctggcg gtaacttacc cattacctcc agagggtagc
     3181 cgtttagtgg ggcagtcgta tactgtaact gttcctgatc acaataccca gccgctggag
     3241 acttttgccg cacaaaacgg gcaagggtta agtaacatgc tggaagcgaa cccgggtgct
     3301 gatgttttct tgccgaagtc cggctcgcaa ctcaccattc cgcagcaact gattttgccc
     3361 gccactgttc gtaaagggat tgtggttaac gtcgctgaga tgcgtcttta ttactaccca
     3421 ccagacagta atactgtgga agtctttcct attggtatcg gccaggctgg gcgagaaacc
     3481 ccgcgtaact gggtgactac cgttgaacgt aaacaagaag ctccaacctg gacgccaacg
     3541 ccgaacactc ggcgcgaata tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg
     3601 ggccccgata atcccatggg gctgtacgcg atttatattg gcaggttgta tgccatccat
     3661 ggtaccaatg ccaattttgg tattgggcta cgggtaagtc agggctgtat tcgtctgcgc
     3721 aatgacgata tcaaatatct gtttgataat gttcctgttg ggacgcgtgt gcaaattatc
     3781 gaccagccag taaaatacac cactgaacca gatggctcga actggctgga agttcatgag
     3841 ccgttgtcgc gcaatcgtgc agaatatgag tttgaccgaa aagtgccatt gccggtaacc
     3901 ccatctttgc gggcgtttat cagcgggcaa gaagttgatg taaatcgcgc aaatgctgcg
     3961 ttgcaacgtc gatcgggaat gcctgtgcca attagttctg gttcaagaca gatgttttaa
     4021 gagcgttggt aattagaaag caaaaagcct gctagaaagc aggctttttt gaatttggct
     4081 cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc taccgactga
     4141 actacagagg aatcgtgtga acggggcgca tattaatggc agtgcatggt gatgtcaaag
     4201 ggtgaaatgc atttttttgt ttgtttgctg tcaatttcaa caaagtggtg agctttcaat
     4261 cttattgggt aatgaaccat cgtggtgcat accctccttt tatagggcag gggaacgcga
     4321 cagctgatta aaggagcaaa tgacgaatct atcataatct cttatttgac agttggttag
     4381 cttgtacatc aacgccaaaa taaaactggc aagcatcttg caatctggtt gtaagtaatg
     4441 gcggcacttg ggccgattct taaaaaccgg aggcaacatg aacttcagac gcctgaaata
     4501 cttcgtaaaa attgtagata ttggtagcct gacccaggct gctgaagtat tgcatatcgc
     4561 acaaccagcg ctcagccagc aggttgccac actggaaggt gagttaaatc aacaactttt
     4621 gatccgtaca aagcggggcg ttacgccaac agacgccgga aaaattctct atacccatgc
     4681 gcgggccatt ttacgtcagt gtgaacaggc ccaactggcg gtgcataacg ttggtcaggc
     4741 attatcgggg caagtctcga ttggctttgc accaggaacc gctgcgtcat ccatcaccat
     4801 gcccttatta caggcggttc gcgctgaatt tccggagatc gttatctatc ccgagcattg
     4861 ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt gagcggcagg cccggtgagg
     4921 aagtcaaaca gcggctacgt actggtactg tggtaaccac agatgacagg aactgggaat
     4981 tacgttactc agcttctgca cttcgtttta acttaagccg ggccgtagca attgatatgg
     5041 aaagtgcaac cattgccgcg caaggatatc gtttccgcgt gccatacggg acactactgt
     5101 gtgtttcaga taaaccgttg catggcgaga ttaaacttcc cggccaggct aaccgttttt
     5161 atgaaggcgc tatttccgaa catctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg
     5221 cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt taatgagccg ccgttccgat
     5281 aataagttct gaaccttctt atcagaaata gatgtgaagg agtaagtaag acacctggca
     5341 aatagcctgc aataatcgtg ggctgtttgc ttccttgggc ggatacgtgt tttattatcg
     5401 tcttaatgat ttccacatat taaaagcaag tatgctttca aaacacaatt ataaaaaatc
     5461 ccgccaacaa tataagtttt tataaaatta aatataagat tatggcttta gaatattttt
     5521 atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa
     5581 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
     5641 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
     5701 tcggtgtaga aatctatgct gctgctaaac aaggtgaacc cgatccagaa ttaaacacat
     5761 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
     5821 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag
     5881 gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat gttaaccgta
     5941 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
     6001 ctgtcagcta tatgtttgac aatactggtg tgattgtatt taaaggatca gaccctgacc
     6061 atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact gaagaagaag
     6121 gtaacattgt tatttatacc gaacctactg accttcataa aggaatcgcg gctctaaaag
     6181 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
     6241 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
     6301 acgatgtaca aaaagtttat cataacgtcg caaatctcta attatatatt aaagaaatct
     6361 gtctttatgg cagatttctt taatctcata taatccttat aaaaaatata atgttcaact
     6421 cgtcatattg cttataaaaa atcctcgttc ccagagaaat aatatttatt ataattccag
     6481 ttcttctttt tctgattaca gaaggcaaag tgacaattac gcatagtttc ccgataaaga
     6541 cgcgacagct acatcccgca taaggcattt ttctctttat ccttgtacgg tacttcatgg
     6601 aacagagttt ttgaccttgc gaatcgtgat gtctgttggg gaggggcaat ttgctcactg
     6661 aagcgtgaga ctcgattaag cgcacgaaac acagaaatca aaaaacccgg gcactttttt
     6721 acaaggtaac cgggtaaaaa taatttttat tttttaactg ttttgagact catagagatg
     6781 tctcaaaact aaaatttggc tcctctgact ggacttgaac cagtgacata cggattaaca
     6841 gtccgccgtt ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga
     6901 tgcccaccca caatgtcaaa gcctgttatt taaatttgaa atcgtttgct gaaataatct
     6961 gcattttgtc gtttattccg acacaactgg ctttttttca cacttttgcg gttcggatcg
     7021 agggtatttc cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac
     7081 agcccagtcg gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgatgcc
     7141 cagcacataa ccgacgacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc
     7201 gtaacgggca tcgcgagcac ctttaaatcc agcgggtagc ccccatgagg cggaccaaat
     7261 aggcataaat aaagcattta gccaaatcag aatcacaacg acatgtttaa cctgtggatc
     7321 ctgggtgtaa aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt
     7381 taatccaaga gtggaaagcc agaacacatg ccgcaactga atctctgctt gcgctatctg
     7441 ccctaccccc aaccttcggc ctgtaatgat cgtagaagca gagccgagcg cacttccggg
     7501 taagttgata agagccgcaa ttgaaaacgc gataaaattt ccggcaataa cactggtccc
     7561 catcccggca acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac
     7621 actcgcggga ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa
     7681 atagctcttt aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat
     7741 tgcaactgcg ccaatataac gagaaatggt taaacccagc cctgccccga caaatcccag
     7801 tcccggccag gagaaaaggc cgtaaatcaa tatgccgcta ataataatat taagaatatt
     7861 caggctaccg ttaatcaata gcggtatttt cgtattccct gcaccacgaa gtgccccgct
     7921 accaataaga gtgatggcag ctgctggata actgagtacc gtcagctcca gataagtcaa
     7981 cgccagtact ttaacttctg tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc
     8041 aaaatgatga ataagcgttg ccaacagtac ggcaaacaac gtcatgatca ccaatgactg
     8101 ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac
     8161 agtagtacca agatcgatag cagcaaaaaa agccataatg accatattga agctgtccgc
     8221 caatcccacg ccggccatcg catcttttcc cagccagctg accagaaaag tgctcagaac
     8281 ccccatcaac aggacacagg cattctccat gaagatagga acagcaagcg gggttatctc
     8341 gcgccagaac aacactttgt agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac
     8401 ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat tgcagaaagt gaaaccacat
     8461 ttcaaataat gagggagaat cagcaaagct gcaaagattt tcgccaacaa attgtctgca
     8521 aatgcaacaa actgttgata gaaacggcaa acagttgggg aatttaaaaa tcgggtttga
     8581 caaaagattt ttcgccgtta agatgtgcct caacagcgat tcctctgtag ttcagtcggt
     8641 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     8701 ccgattctta cgctatctta ttagtcctta tttgatattg attcaataag ttagcgtgaa
     8761 aatctttcca gatgtgtatt cagttttccc tccgcatccg gttaatttgg tggtcagatt
     8821 tgggggcagg ttggttcgat tctggagtga cccccaaatg tctttaaacg attcaaaaat
     8881 ccgtaactta aaaccctctt caaaacccgt aaaactgtct gattcgcacg gtctgtatct
     8941 gctggtcaat cagggcggtt cgcgtatctg gtatctcaag tatcgcttca gtggtaaaga
     9001 gtcccgcgtc agtctgggcg catatcctct ggtttcactt gctgaagcaa gacaacgacg
     9061 tgacgatatc cgtaagctac tgacgcagaa tattaacccc gcccaccagc gcatgtctga
     9121 taaagccgca gcgtcaccgg aaaagtattt taaagccgtg gcgcttgcat ggcacaaaac
     9181 caacaaaaaa tggtcgcctg attatgccgc gcgcatcctc gccagcatgg aaaatcatat
     9241 ctttccggcg attggtcatc tgcctgtcac tacgctgaaa acgcagaatt tcactgcgtt
     9301 actgcgggtt attgaggata aaggttttct ggaagtcgcg tcccgaaccc ggcagcaact
     9361 ctgcaacatc atgcgttacg ccgtacagca gggattcacc gaaaacaacc tggcgcagca
     9421 tctggaaggc gtcaccgcgc cgccggttaa gaatcactat cccgccctgc cgctggagcg
     9481 gttgcccgaa ttgtttgagc gcattggcga ctatcagcag ggacggcagc ttacgaggct
     9541 ggcggtggtg ttgactctcc acctgtttat ccgttccagc gagctgcgct ttgcccgctg
     9601 gggtgagata gatttcagaa acaaaatctg gaccatcccc gccacaaggg aagcgattga
     9661 gaaagtccgt ttctctggcc gtggcgcaaa aatgcgtact ccatatatag tgccgctttc
     9721 tcgtcaggct atcgccattc tgaagcagat aaaggaaatc tccggccatc tggaactggt
     9781 gttccccggc gaccataacc cgtataagcc gatgagcgaa aacactgtca accgggcgtt
     9841 gcgtcttatg ggctatgata ccaaaactga tgtctgtggg catggattcc ggacaatggc
     9901 gtgtagcgca ttagtggaaa ctgaactctg gtcacgggat accgtggagc ggcagatgag
     9961 tcatcaggag cgcaacagcg ttcgggcagc gtatatccac cgggctgaac atctcgacgc
    10021 ccgtacagcc atgatgcagt ggtggtcgga ttatctggat gtaagtcgcg aggggtacgt
    10081 tgcgccgtat atttatgcgc ggcggcataa ggcggcctga gacagtggct atataatttg
    10141 ttaaaaatga aaaagcagat cctgattgaa tccggatctg ctttttacat taggcaatcg
    10201 ctgccagaca atctgcacca gaaacgccat aactgcacca gttcagtacc accgcttacc
    10261 tgcgccagtc tgccgtcgcc tgtctgtgtc aggactgcgc cagatcttat cgaataaaca
    10321 tcaaactgcg ccagtgttac ggccctttca gccctttggc cttttccggt ctggcgctgt
    10381 tacccttagc cgccccttac cctttccggc ctttttcctt tgctggcgca atcgccagaa
    10441 aaatcgtcaa gctgcaccag aagcctgcca ataggtcata ccgttcagaa gcatcgttat
    10501 tgagaggaaa ccgtacaaaa aatttctacg tagaaatttt ttcggtggat aacgatgaat
    10561 aaagagtatt ttataatgtg atggctatca aaaatcgacc atccaaaaat aattcattga
    10621 tttataatga ataaccatga atttaatagt cgatgattat tgtttttatc agtattgata
    10681 ggaaagtata caaaaaattt tggccccaaa attttttgta cgaaaaccga tgaataacgc
    10741 gaaattaata ggaaaccatc caaaattatt tggggcaaat aattttgccc gaaaaccgat
    10801 ggataactcg atcttttcaa agcaacggct atcaatcagt atcctttctt ccgtaaacct
    10861 gcgccacata ccgcccgcgc ccgtcgccgt gtcccaaatc catcgccgtc attgccagcg
    10921 cttctttctc actgaatccc tgcgccagat aatgacgaat ggcatcctgc gcccacgcat
    10981 agcgcaacga gtgcggggaa tatgtgccag ttaacccgag acgtgacgcc tggctgtgcc
    11041 agtatttcat cgcgcttttc aggtcgggtt tatctatcag cctgccgtgg cgatcttctg
    11101 ccacaaccaa cgcattatcc agcgcttttc tgatagcgac agcatccaga ataacggttt
    11161 ctcgcggacg cccgcctttg gtgccgaaca ccacggttaa acggggctca ccccgctcca
    11221 gcgattgctt ccacgttctt aatgactgcg cactttgcac cacctcctgc gaacgtaatc
    11281 ccatcagcct tgagagttcc agcgccgctg ccatccccgg atcttttgcg cgggcggttt
    11341 ccagcacata gcggtaatgc tccggcgtga tggcccgctt cgtaccgtta cgggatgcgc
    11401 cagacaggcc cagcgagcgg ttattcagtc gctcactctg cgccagcctg tcacgcccgg
    11461 cctgtttcag aatgcagcgc accgccgcca tctcattttg cagggaacgc ttcgtgatac
    11521 cctgcgccag tcgttcacga acatatccct caatataccg cgccttaagc tgctccaccc
    11581 ggcggatctg cacattctgt gccagaacca gacgctcaca aaaccgctgc accagcttaa
    11641 tacggtcgtg aacggtctta tggctgccgc ccgcctgttg tgccaatcgc ttcatttcct
    11701 gctccagaat ccccatacat tccccctttc atgattgccg caaactcatc cccaaaaact
    11761 caacactgat acagcgttcc ccggcgcaca ggccgatagc ccctgagttt tgtcggggct
    11821 gcgaacgata cctgagcgcc ggggcggcag ccgttgaggt gtggtggggc ttcggttgtg
    11881 ctctcagagc agccgcccgc ttgcgcgggt catcccccgg atcgtccaga tccgcctcgg
    11941 tatcggttca tattctcctt atgggtaaat ggcctaaaaa ggcggtgtca gaatccagcc
    12001 atgagctggc gcagttgagt tttctggtgc aggtcgaaag ggcttgcgtg cgtcactttc
    12061 ccgctttgcg cggttaaagt gacgcacgga tctggtgcag catctgagat ttatcgtctg
    12121 gtgcagtttt acggtcacgc ggcagcaaag cctgacggca atgcaggccg cagcccgtaa
    12181 ggtaaaaggc gctgagggtg tcagcagatg caggttagcc gggagaattc cggcgcggtg
    12241 cagattcaga ccagcaggct ggcgaagtaa aaggatgtat cgtattaaaa ctctggcctc
    12301 cgtcgcgatg ttctgcgacc cggtgtttgc agaggtctgc accggtaaac ggatcgaccg
    12361 tcctgataac aggcgcttct ttcaggttgt gaagcgttgg gcgctgtcta cgacagcata
    12421 gagcaagggg gagactacgc ctgtgccgct ggcgcggaaa ggagaaactg tttttacctg
    12481 caaaaggatt ttttaatcac ttgttgttat aaaaaatgag ggcgctgtat gtgacagcgc
    12541 cctcgttcag gttacataaa aaatatcagc tcagaatagc ttacagtcga gccagcacct
    12601 cattcacgac agcatcgggt atacgttcca gccgcttacc gctccgagct cccatatcaa
    12661 tggttctggg ctggtcgcag cgaataacgc ccgttgtttt tgttcccgcc ccgtccagtg
    12721 aaacggtaaa accggcttcg cgggcaaagt ttccgccgct ggtgacaggg acaacaacag
    12781 gcaaccgggt gaacgtgtta aacgatgcct tcgatacgat gagtaccgga cgttttccac
    12841 tttgttcatg gccagtggtg ggatccagtg aaacgagcca gatttctcct ctgtccatct
    12901 acaggatctc cttgcccacc gcaggcgcat caatccattc ccgaccctct tcgcttattt
    12961 cagcgtgcgg atcgcactgc gccaacagtt cttcgagcga atagcgggga cgtttttgag
    13021 gttcaataat caggcagcca ttatcaatgg tcatgcccac ttcgctgtcc gtcgacagcc
    13081 ctagcgtttt cagcactgcg gggggaaccg ccagcatgac ggatccgcca acctttttca
    13141 ggcgagtggt atacatagag cacctccgga tattatattt taatataaca tccatgatag
    13201 acgatgcaca actattcatc agattcattg tggtttcctt agtgtaataa agcgtgaaga
    13261 aagccttaat ccatcggaag acagatccgg cagttttcaa aggcgacatt gccggtaaaa
    13321 gggcaaccgt cctgatacat caggcgcttc tttcaggtca tgaagcattt cgtacaggcg
    13381 ggagcttagt gcgtaacaaa gaaggccgat aataggaaac tgcttttgca gttcaaagga
    13441 ttatgacggt tcggataaac aaaatgcggc gcagcttccg tatccgggct gcgccgcagg
    13501 aacatcagcg gtagcgcgaa ggccagattt gctcaggctt gatgcctaac gcttcggcaa
    13561 ttaacctttc gcctttgggc cagcggcggg taagcgcatt agccagcgtg gaggacgcca
    13621 gacctgcctg acgcgaaagc gctgacagcg aagtgcctcg ttttttcagc ccggcgataa
    13681 tgtcggcagg atgccagtcc tgcagcatca tgcagccacc tcgtcgataa acttcacgcc
    13741 atcggcggtg cggagccagc gcggggcttt catttcggcg gcaaaatacc agaccagttc
    13801 acataataag cctgttatcg tctgctccac ttttggctgc atcccctgcc cgataagaag
    13861 ttgcgccagc gtcagtgagt aatcacaaag taattctgcg tcggggacaa atcggggcat
    13921 atcggcaggt agagcatcaa cggtcaggct gtcagtcaga tgagacggga tggggtcgtt
    13981 cagcgtgggt tgcagcaaag tgagacaggc ggcaagtcgc ccgcaaagcg ccagccgcag
    14041 caccggatct tcgctttcga tcagggtttc ggcgacgttt tcgcagtggt ctgccagtac
    14101 ggtaaaatct gtggcagcac tgaagggaac agcaagtaac gggtgtgtct gagtggggat
    14161 cgtagccata aaggcagcct ccagtatgtg gtttgaaaac caccaccaga ggttccaatc
    14221 tcatgggtgg tggactgaac gaggttggaa ctaccggcca tactggaacc ggcgcacctt
    14281 acggtgcccc cgcccagccc accataattt tggtgtagct aagcgcgcga cataaaaaaa
    14341 gacgcagacg cgtcacgtgt cgccagtatg gttatatttc ggggttccaa tcccggcacc
    14401 tgattttgca ggtgcgggta aaagataccg ctttcggttg aaaacggcaa ggggaagtcg
    14461 tggaatagag atcgcagatt gttataaata tacacaatag ttaatgttaa caaagacggc
    14521 acatatatta actttactct caatctgctg aatagtttat cgcatgtaat tctcgggtca
    14581 tatagtggat accctccttc ttttctttca tcccctcttg cctcaccata aaagcaaacg
    14641 aaaaaattgc tgaacgagtt atctgaagta aatgctcaaa atggtgaata aaatcacttt
    14701 ctttgataaa aagaatatct tctattagtt gataagattc gtaaatgtcc tcaggtttat
    14761 cacttttatg tacaactaaa aacttatgct ctaaaccatt gcgccattct ttatagaaag
    14821 ccaactcccc acccttatct ttattcaggt cggtagcaat actgtacagc gccagaagcc
    14881 ctggtgattt taccttttca aataattgtc ttctgttatc aacatctaac tgccagaaat
    14941 tctgaaaata gatattcttc tgaggtgtac ctttttttgt tgttgggtaa agattataaa
    15001 gttcacatat ggctaccgca attttatcaa gaataccaaa gcaaagacga aatgccgtcc
    15061 tgattttttc aatttctatt cctaaaactt catcattata caattccaga aaatggcttt
    15121 cgtacttaat gtaatccgta ttttgtggat gaagataatc aaaataaaga tggcgtgcga
    15181 gtgaaaactc cgatttaagg cgatttaata ccatctccat gggaatgata aaatcacctg
    15241 ttacaccgcc tcgcgaagca atcacaagat catctgtagc actccctaca catgggcaat
    15301 acaggccatg ttctgataaa gtcagatgat ttctaagaca aaactgtcga tatgggctta
    15361 acacttcgaa ctcttgttgt gtaagtgctt catcgtgttc gtcagtttca tcatcaacag
    15421 catattctgc gattttctcg ttagtctgcg ctatgcgttc taaatagaac gattcccatt
    15481 gtggtgggat attttttgat acagacgctt taatatatcc tgctctcact tcttttagta
    15541 acttgattga aaatgaagat gaaataagat tgaggaccat aagtgacatc gaacggttga
    15601 cccaagcctg tggtatatct aatcccttaa gattggtaag atcataatat ctcaatgact
    15661 cactgagccg aaattgactt cttaatgaat tagccaaatt aacagatagt tcagcctgaa
    15721 attcctcctg ctcctcagca gatagcttaa aagaacgcca gtagatattt tttaaggaaa
    15781 ccaattcttc tatcgatttg aatgtatggt cattggggtt agtaatgctc accagattag
    15841 atagcgcatt accatagtta taatagtaaa ggcactctcc taacactgat ataaatgctt
    15901 ctttgttatc ttccatcagg ttgcaaccaa gctctgacgc atttttatta ttttgcatct
    15961 gcccgatgtc aacaagagca cccgcaatgt tcgccaatac tataaagtag ttatcgttcc
    16021 tgattgttat tctttccagt ttgttcagga acgcaactga ctgttgataa gattcgtcaa
    16081 atcttccaat atcaaccagt tggctaatga aattactttc atcgacaagt tcttttgtta
    16141 tcaaaataaa ttttccttat acagaggatt tcggtgcttc aacaggaggt atgccctcac
    16201 aggagacatc tataactatc gctgataagc ggtctctttt atcaaccggc acagtttcat
    16261 taagcatatt ttccattgca ctggcgtttt caggttgaga tgtatttttc gccaaatgag
    16321 gcagggaatt cggtcgagca gaaccaaacc aaaaaaccag atagataccg tgaccagcag
    16381 catcggggtt tttagtataa agcttgtcca attgcccatt aagtgcagtc cagacatcac
    16441 gatgataatc acgctttatc tcgataggta tttttatctg cggtagtgaa acaatgatat
    16501 ctgccctttt atcagaaacc atgtgtcctt ctggttcaca gcttattttt tatggattaa
    16561 gccggacacg gagtaaatcc agaaaaatat tacggcaact ctcttcaggt ttaggcgtta
    16621 gggtacggcc atagctgtct tcattccaga attgcttata aatatcggta ttttcattgg
    16681 caattcggtt agaaatatcc cgtatatgat caagcaataa tgagtaaaga tccattatgt
    16741 tggcaggcgt tttattcatc aatgtactta cagtctgcct ccagtcggca tgatggaact
    16801 gtgatt
//
LOCUS       DAJTYD010000076        16607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTYD010000076 DAJTYD010000000
VERSION     DAJTYD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.076"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..293)
                     /gene="hlyE"
                     /locus_tag="OO585_002253"
     CDS             complement(<1..293)
                     /gene="hlyE"
                     /locus_tag="OO585_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6629827.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAY"
     gene            666..1085
                     /locus_tag="OO585_002254"
     CDS             666..1085
                     /locus_tag="OO585_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6629828.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1085..2352
                     /gene="umuC"
                     /locus_tag="OO585_002255"
                     /pseudo
     CDS             1085..2352
                     /gene="umuC"
                     /locus_tag="OO585_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2398..2928)
                     /gene="dsbB"
                     /locus_tag="OO585_002256"
     CDS             complement(2398..2928)
                     /gene="dsbB"
                     /locus_tag="OO585_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6629829.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3074..4615)
                     /gene="nhaB"
                     /locus_tag="OO585_002257"
     CDS             complement(3074..4615)
                     /gene="nhaB"
                     /locus_tag="OO585_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6629830.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4837..5556
                     /gene="fadR"
                     /locus_tag="OO585_002258"
     CDS             4837..5556
                     /gene="fadR"
                     /locus_tag="OO585_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6629831.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5608..7140)
                     /locus_tag="OO585_002259"
     CDS             complement(5608..7140)
                     /locus_tag="OO585_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6629832.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7470..8768
                     /gene="dadA"
                     /locus_tag="OO585_002260"
     CDS             7470..8768
                     /gene="dadA"
                     /locus_tag="OO585_002260"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6629833.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8778..9837
                     /gene="dadX"
                     /locus_tag="OO585_002261"
                     /pseudo
     CDS             8778..9837
                     /gene="dadX"
                     /locus_tag="OO585_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(10224..11960)
                     /locus_tag="OO585_002262"
     CDS             complement(10224..11960)
                     /locus_tag="OO585_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6629834.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12055..12969)
                     /gene="ldcA"
                     /locus_tag="OO585_002263"
     CDS             complement(12055..12969)
                     /gene="ldcA"
                     /locus_tag="OO585_002263"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6629835.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13069..13680
                     /gene="emtA"
                     /locus_tag="OO585_002264"
     CDS             13069..13680
                     /gene="emtA"
                     /locus_tag="OO585_002264"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6629836.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13682..14416)
                     /locus_tag="OO585_002265"
     CDS             complement(13682..14416)
                     /locus_tag="OO585_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6629837.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14617..14871
                     /locus_tag="OO585_002266"
     CDS             14617..14871
                     /locus_tag="OO585_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6629838.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15050..15490
                     /locus_tag="OO585_002267"
     CDS             15050..15490
                     /locus_tag="OO585_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629839.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15569..16607)
                     /gene="treA"
                     /locus_tag="OO585_002268"
                     /pseudo
     CDS             complement(15569..16607)
                     /gene="treA"
                     /locus_tag="OO585_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
BASE COUNT         4183 a         4097 c         4303 g         4024 t
ORIGIN      
        1 atatacgctg cgagcaattg cgtcgcaaca ccacaccatt catacactgt ttgggttgct
       61 tcaaaatact tatcctggct atccataagt aaggttttaa tatcgccgac taaaacggag
      121 gctgcctgtg aatactcctg tttaaagcga cttaactctt ttatggtttc atcaaaggtc
      181 tgccagggga tgacctgatc gagatattta ttataaagat ctaatgctcc atctgcggtt
      241 tcgattgcgt ttttaactac ttctaccatt ttatctgcaa cgatttcagt cataatcatt
      301 cgcctcttta aatatataaa ttgtaatgaa actcctgttt tacaactatt aataaatttt
      361 acttcatcta attcatagtt agccgggtgg gatgcgtcaa tgtctttatt tctattaata
      421 tgataaatat caaacaatgt ttaatgtcat tatggcgaat gcttctattc tatttttttg
      481 ccgggtgata tttttcattt ctgctggatg agcgtcgtcg ccagaaggcc acgtgagcac
      541 aggataagag aacgaaaaat cagcagtcta tacagcgaca aatattgata gcctgaatca
      601 gtattgatct gctggcaaga atagactact gtatataaaa acagtataac ttcaggcaga
      661 ttattatgtt gtttatcaag cctgcggatc tccgcgaaat tgtgactttt ccgctattta
      721 gcgatcttgt tcagtgtggc tttccttcac cggcagcaga ttacgtcgaa cagcgcatcg
      781 atctgaatca actgttgatc cagcatccca gcgcgactta cttcgtcaaa gcaagtggtg
      841 attctatgat tgatggtgga attagtgacg gtgatttact gattgtcgat agcgctatta
      901 ccgccagcca tggtgatatt gtcatcgctg ctgttgacgg cgagtttacg gtgaaaaaat
      961 tgcaactacg cccgacggta cagcttattc ccatgaacag tgcgtactcg cccattacca
     1021 tcagtagcga agatacgctg gatgtctttg gtgtggtgat ccacgtcgtt aaggcgatgc
     1081 gctgatgttt gctctctgtg atgtaaacgc gttttatgcc agctgtgaga cggtgtttcg
     1141 ccctgattta tggggtaaac cggtggttgt actatcgaat aatgacggtt gcgttatcgc
     1201 ccgaaacgct gaggcaaagg cgctgggtgt taaaatgggc gatccctggt tcaaacaaaa
     1261 agatctgttt cgtcgctgtg gtgtggtttg ctttagcagc aattatgagc tttacgcaga
     1321 catgagcaat cgggtgatgt cgacgctgga agaactatcg ccccgcgtcg agatttacag
     1381 tattgatgag gcattctgcg atctgacagg tgtgcgtaat tgtcgcgatc tgactgattt
     1441 tggcagagaa attcgcgcaa cggtgctaca acgtacccat cttactgttg gtgtggggat
     1501 cgcccagacc aaaacgctgg ctaagcttgc caatcatgcg gcaaaaaaat ggcagcggca
     1561 gacggggggg tggtggattt atcaaatctg gaacgccagc gtaaattaat gtctgctctc
     1621 cccgtggatg acgtctgggg gattggacgg cggatcagca aaaaactgga cgcgatgggg
     1681 atcaaaaccg ttcttgattt ggcggataca gatatccggt ttatccgtaa acattttaat
     1741 gtcgtgctcg aaagaacggt gcgtgaactg cgcggtgaac cctgtttgca actggaagaa
     1801 tttgcaccga caaagcagga aattatctgt tcccgctcgt ttggtgaacg tatcacggat
     1861 tatacgtcga tgcggcaggc catttgtagt tacgctgccc gggcggcgga aaaacttcgc
     1921 agtgagcatc aatattgtcg gtttatctcc acatttatta agacgtcacc atttgcgctc
     1981 aatgaacctt attacggcaa tagcgcgtcg gtaaaactgc tgacgcccac tcaggacagc
     2041 agggatatca ttaacgccgc tacgcgatct ctggatgcca tctggcaagc gggccatcgc
     2101 taccaaaaag cgggcgtgat gctgggggat ttcttcagtc agggagtcgc gcagctcaat
     2161 ttattcgatg acaacgcacc gcgccccggg agtgagcaat tgatgacggt aatggataca
     2221 ctgaatgcta aagagggcag aggaacactc tattttgccg ggcaggggat ccaacaacaa
     2281 tggcagatga agcgagccat gctttcacca cgttatacaa cgcgaagttc tgatttactg
     2341 cgggtcaaat aaatatagcg gcaggaaaaa agtgctcccg caggagcgcc gaatggatta
     2401 gcgaccgaac agatcacgtt ttttcgcttt aaacggctgg gaaatcatca ccagcaccgc
     2461 gacaatcagg taagcgataa aaataccgag cagccactgc ggcatttcca gacctaaaaa
     2521 atcccactga cgctcggcgc aatcgccaga ggcgacaaac acttgcggca cccatttatc
     2581 cagcggcagc cattccggga aacgaaccat aaaatcacag gtggcaaacg gcgaaggata
     2641 gagctgaagc atggtgtgct cgtaagttaa ctgcacaccg cggaacgcac tatacaacca
     2701 gataaccatc gctacataac gcagcggagt tttcggggcg atcgcgccaa tcagcgcagc
     2761 acccagaacg ccgaataacg cgcagcgttc ataaatacag agcacgcaag gtttcagtaa
     2821 catcacatgc tggaaccaca gcgccgtcag ttccagtgcc agagcagtaa acgccatcaa
     2881 cagccacgcg ccccggcctt gtgaacattg gttcaaaaat cgcaacataa tcatttccct
     2941 gcaatatgca tagagtgcgc agtttaaacc aattcattcg ctgcgccacc aggaggcagc
     3001 aaaaatagga cgcaattgga caattatcgg gcgaaaaggc aaaccgggca tttatatgcc
     3061 cggtaagttg ttatcaaagc gttgctatcc agcccatttg cataaaccat tcggttacag
     3121 gggcaagcgt aaactcgacg cagagcagcc cgacgagtgt caggacgagg gtgtaaggca
     3181 gggccatcca caccatgcgg ccataagaga ggcgaatcaa tggcgcgagt gcagaggtca
     3241 gcaggaacag gaacgcagcc tgaccgttcg gcgtagcgac agagggcaga ttggtaccgg
     3301 tattaatggc gaccgccagc agctcgtatt gcttcaacgt gatagcgcca ctttccattg
     3361 ccgctttcgc ttcgttgata taaatcgtcc ccacgaagac gttatccgaa atggatgaca
     3421 gcagaccgtt gaaaatatag aacaacgaca gctgagcatg ttccgatgcc tgcaacacaa
     3481 actgaataat cggcgaaaac agttgttggt cgataatcac cgcgactacc gagaaaaaca
     3541 ccgtcaacag tgcggtgaat ggcagagatt cggtgaaggc tttaccgata gcatgttcat
     3601 cggtgacacc ggtcaatgat gtcgccagaa taatgaccga caaaccaatc aagccaacct
     3661 ccgccagatg caacgccagc gcagtcacca gccagacgcc gataatcgcc tggacaatca
     3721 gacggatttt atcctgacgg gtacgctggt ggcggctttg atcgtcaaac tgttgcaaca
     3781 cttcgcggac tttctccggc agcgtttcac cgtagccaaa ccaacgcagc ttctctacca
     3841 gcaggcaggt taacaggcca caaatcagaa ccggaacggt caccggcgac atgcgcagga
     3901 agaaatcgcc aaaatgccag ccagccgctt tagcgatgat caggttctgt ggttcgccca
     3961 ccatggtcat tacgccgcct aatgcggtgc cgacaccggc atgcatcatc aggctacgca
     4021 gaaagccacg gaactgttcc agaaccactt tgtaatgctt gtcgatatga ctatcgtctt
     4081 gcaggtcggt atcttcagta cgggaagagg ctacgcggtg ataaatacca taaaaaccga
     4141 ctgcaacgct gatcaccacc gccacgacgg ttaaggcatc gaggaacgcg gagaggaacg
     4201 ccgccgccac gcaaaaagag agcgacagca gcattttgga acgaatgctt aacagcaaac
     4261 gggtaaatat gaacagcaat agctgtttca taaaatagat acccgccacc ataaacatca
     4321 gtaacagcaa gacttcaaga tttgccgcca cctcttcacg gacgtgttcc gcgctggtca
     4381 tgccgatgaa taccgcttca atagccaaca gaccaccggg gagtagcggg tagcatttca
     4441 gggccatcgc cagagtgaaa ataaattccg ctaccagcaa ccagccagcg acgaaagggc
     4501 tgatgaggaa aattaacgga tttacgatta agaaaatgat gagggcgagt ttgtaccagt
     4561 cgggggactg gcccaaaaaa ttgcgccata gcgcgcggcc ccaggagatc tccatgatgg
     4621 tttcccttac cttacaaata atcaatgatg tttttatgtt taaacgcaaa gcttaacggt
     4681 caggcaggag tgaggcaagt cttgatagtc aaggggaaag agatgcggaa aatgaagcct
     4741 tgatcccttt tttcttcttt ttgtctgcta tcagcgtagt tagccctctg gtatgatgag
     4801 tccaactttg ttttgctgtg ttatggaaat ctcactatgg tcattaaggc gcaaagcccg
     4861 gcgggtttcg cggaagagta cattattgaa agtatctgga ataaccgctt ccctcccggg
     4921 actattttgc ccgcagaacg tgaactttca gaattaattg gcgtaacgcg tactacgtta
     4981 cgtgaagtgt tacagcgtct ggcacgagat ggctggttga ccattcaaca tggcaagccg
     5041 acgaaggtga ataatttctg ggaaacctct ggtttaaata tccttgaaac actggcgcga
     5101 ctggatcacg aaagtgtgcc gcagcttatt gataatttgc tgtcggtgcg taccaatatt
     5161 tccactattt ttattcgcac cgcgtttcgt cagcatcccg ataaagcgca ggaagtgctg
     5221 gctaccgcta atgaagtggc cgatcacgcc gatgcctttg ccgagctgga ttacaacata
     5281 ttccgcggcc tggcgtttgc ttccggcaac ccgatttacg gtctgatcct taacgggatg
     5341 aaagggctgt atacgcgcat tggtcgtcac tatttcgcca atccggaagc gcgcagtctg
     5401 gcgctgggct tctaccacaa actgtcggcg ttgtgcagtg aaggcgcgca cgatcaggtg
     5461 tacgaaacag tgcgtcgcta tgggcatgag agtggcgaga tttggcaccg gatgcagaaa
     5521 aatctgccgg gtgatttagc cattcagggg cgataatccc ttccgtttaa aaagcaaacc
     5581 cctcaaacga ggggtttttt gttgttttta cagatttccc attcttggcg ggcaacgttc
     5641 cagcaactcg acgctgccat cttcgttttg ctgttccagc atcacatcaa atccccacag
     5701 gcgatgcaca tgcttcagca cttctttgcg cccccgatcc agcggtgcgc gattatgtgg
     5761 aatgtaacgc agcgtcagcg aacggtcgcc gcgcaaatcc acgttccaga tctgaatatt
     5821 cggctccaga ttacttaagt tatattgcga cgataaccgg ttacggatct cccgataacc
     5881 ttcttcatta tgaatagcgg aaatctccag ataattatgc cgatcgtcat ccagcacggt
     5941 gaagaagcgg aaatcacgca tcactttcgg tgacagaaac tggctgataa aactctcatc
     6001 tttgaaatca cgcatcgcga aatgcagcgt ttccagccag tcggaaccgg cgatatccgg
     6061 gaaccagtat ttgtcttctt ccgttggcga ctgacaaatc cgtttaatat cctggaacat
     6121 ggcgaacccg agggcatacg ggttgatgcc gctgtaccac gggctgttat aggggggctg
     6181 gaagaccaca ttggtgtggc tgtgcaaaaa ctccagcata aaacgttccg ttactttccc
     6241 ttcatcatac agatggttaa ggatggtgta gtgccagaag gtggcccagc cttcgttcat
     6301 cacctgagtc tgtttttgcg gataaaaata ctggctcacc ttacgcacaa tacgcaggat
     6361 ttcacgctgc catgattcca gcagtggcgc atttttctcc ataaaataga gcaggttttc
     6421 ttgtggttcg gacggatagc ggcgcgcttc agcaaccgtt ttctcttcct cgcgcttcgg
     6481 cagggtacgc cagagcatat tgacctgact ttgtagatac tcttcgcgac ttttctgccg
     6541 ggctttctct tcttgcagcg agattttttg cgggcgtttg taccggtcca cgccgtagtt
     6601 catcagcgcg tggcacgagt ccagaagccg ttctacttca tccacgccat aacgttcttc
     6661 gcactcggta atatatttac gggcaaaaat cagataatcg acaatcgaac tggcgtcggt
     6721 ccaactgcgg aataagtaat tgtttttaaa gaaagagtta tgcccatagc aggcgtgtgc
     6781 catcaccagc gcttgcatgg taatggtgtt ctcttccatc aggtaagcga tacacgggtt
     6841 agagttaatg acgatttcat aggccagtcc ttgctgaccg tgcttataca gccgttcagt
     6901 ctcgataaac tttttaccga atgaccagtg cggatagtta attggcatgc cgacgctgga
     6961 gtaggcatcc atcatctgtt ctgaggttat cacttcaatc tggtgcgggt aggtatccag
     7021 ccggtagagt ttcgccaccc ggtctatctc tgccagataa acatccagca ggtcgaacgt
     7081 ccagtcgggt ccatcgctca accgtgtggt gtccttattc atagaatcga tcgtcgccat
     7141 acgcgcacct cattgttgtc ggcgctctct gtgtggagca cctcatttca agcatagaac
     7201 acctgttaaa aaccgcgtcg ccggagaatt tttttctttg cgatttctta ttatcagagt
     7261 gccactaatc cgcttctgaa cggaatttta tgctggataa aaagggcgtt cagcaggaga
     7321 tactaaagac gccatattgc cgcagagtca gggagatgtg agccagctca ccataaaaaa
     7381 gctgtatgtt gaataatatt ttcaactgag ttatcaagat gtaattagat tattattctt
     7441 ttactgtatc taccgttatc ggagtggcta tgcgagttgt catactggga agtggtgtgg
     7501 taggcgttgc cagcgcctgg tacttaaatc aggcaggaca tgaggtcacc gtcattgatc
     7561 gggagccggg ggccgccctg gaaaccagcg ccgcgaatgc cgggcaaatc tcccccggat
     7621 atgctgcacc gtgggcggca ccaggtgtgc ctttaaaagc gatcaaatgg atgttccagc
     7681 gccatgcgcc gctggcggtt cgtctcgacg gtacgcagtt tcagttgaaa tggatgtggc
     7741 aaatgttacg taactgcgac accagccact acatggaaaa caaagggcgg atggtgcgtc
     7801 tggcggaata cagccgtgat tgcctgaaag cattgcgcgc cgaaaccaat attcagtatg
     7861 aagggcgtca gggtgggacg ctgcaactgt tccgtaccga acaacagtat gaaaatgcga
     7921 cccgcgatat cgccgtgctg gaagatgccg gcgtaccgta tcagctgctg gaatccagcc
     7981 gcctggcgga agtggagcca gcgctggcag aagtagcgca caaactgacg ggtggtctgc
     8041 aactgcccaa tgatgaaacc ggagactgcc agctgtttac ccagaatctg gcgcggatgg
     8101 cagagcaggc gggggttaaa ttccgcttta atacgcccgt tgaccaactg ctttgcgacg
     8161 gcgagcaaat ctacggcgtg aagtgtggcg atgaagtgat taaggccgat gcgtatgtga
     8221 tggcgtttgg ttcttactcg acggcgatgc tcaaaggcat tgttgatatt ccggtgtatc
     8281 cgctgaaagg ctactcgctg actattccaa ttgcgcagga agatggtgcg ccagtatcca
     8341 ccattcttga tgaaacctac aaaatcgcca ttacccgttt cgataaccgc attcgtgttg
     8401 gcggaatggc ggaaattgtt ggttttaaca ccgagctgtt gcaaccgcgt cgtgaaacgc
     8461 tggaaatggt ggttcgcgat ctctatccac gcggcggtca tgtcgagcag gcgactttct
     8521 ggacgggttt gcgcccgatg acgccagacg gtacgccggt tgtcgggcgt acacgcttta
     8581 aaaatctgtg gctgaatacc ggtcacggca cgctcggctg gacgatggct tgcggttccg
     8641 gtcagttgtt aagcgatctg ctctctggtc gcacgccagc gatcccatat gaggatctaa
     8701 gcgtagcgcg ctacagccgt ggatttacgc catcacgtcc gggccattta catggcgcac
     8761 acagctaagg aaacgagatg acccgtccga tacaggccag cctcgatctg caggcattaa
     8821 aacagaatct gtccattgtc cgccaggccg cgccgcacgc gggtggtaaa agcgaacgct
     8881 tacgggcatg gtattgagcg tatctggagc gcgctcgggg ccaccgatgg ctttgcatta
     8941 cttaacctgg aagaggcaat aacgttacgt gagcgcggct ggaaggggcc gatcctgatg
     9001 ctggaaggat ttttccatgc tcaggatctg gagatttatg accagcaccg cctgaccacc
     9061 tgcgtacaca gcaactggca gctcaaagca ctgcaaaatg cgcggctaaa agcaccgttg
     9121 gatatttatc ttaaagtgaa cagtgggatg aatcggttgg gcttccagcc cgatcgcgtg
     9181 cttaccgtct ggcagcagtt gcgggcaatg gcgaatgttg gcgagatgac cctgatgtcg
     9241 cattttgccg aagcggaaca tcctgatgga atttccagcg cgatggcgcg tattgagcag
     9301 gcggcggaag ggctggagtg tcggcgttcg ttgtccaatt cggcggcgac tctgtggcac
     9361 ccggaagcgc attttgactg ggttcggcct ggcattattt tgtatggcgc ttcgccgtcc
     9421 ggtcagtggc gtgatatcgc caataccgga ttacgtccgg tgatgacgct aagcagtgag
     9481 attattggtg tccagacgct aaaagcgggt gagcgtgtgg gctacggcgg tcgctatact
     9541 gcgcgcgatg aacagcgaat cggcattgtc gccgcagggt acgccgacgg ttatccgcgc
     9601 cacgcgccta ccggtacccc tgttttagtg gacggcgtgc gcaccatgac ggtggggacc
     9661 gtctcgatgg atatgctggc ggtcgattta acgccttgcc cgcaggcggg tattggtacg
     9721 ccggttgagc tgtggggcaa ggagatcaaa attgatgatg tcgccgccgc tgccggaacg
     9781 gtgggctatg agttgatgtg cgcgctggcg ctacgcgtcc cggttgtgac ggtgtaactt
     9841 gttgtaagcc ggatcggagg caacgtcttc tgggtgcaaa aaaaatcatc catccggctg
     9901 gtcagcaact gtagttgtta atgtgacaga gccattgccc atgatagtgt ccattaaaag
     9961 gatggacact atttccccgg aacctgaact caccgcacag gcgttctaca taaaacgctt
    10021 acgcttcatt gttgactcgg agtcgacttc agataaatca cgctttaccc ttgatggagc
    10081 ctgtacatag atttgtgtaa ttgcctgatt ttgatatgtt caattcaaca tcaaatgaag
    10141 gttaaattat ggacgacaaa caattgcagg ctcaggctgc gttcagcaaa gcatcgcaac
    10201 cggcgataga tgcttcatta aatttaagat tcagcttcct cttcagccac ccgtacgcca
    10261 atcttcaaca cttcattatc ttctttctcg gccaccgtcc agatcatccc ggcaaactct
    10321 acctggtcac cgacaaccgg tgccgtgcct aacaactgct ggacaatttc acccagcgtt
    10381 tgctgcttat cacgatattc tcgcccgtct tccaggccat atatcagcgc aacatcagca
    10441 tatttggcgc tggcttcgag aatgaagtca ccaaagaagc gttgatccag cgcgaccggc
    10501 ggcgactggc tgaacagttt accgagcgcc ggaagatcgc gttcccgacc aattacacac
    10561 aacacatcgc cttcgcgcag tcgggtgctg ccggtaggat gaagcaactg gttatcacga
    10621 aacagtgccg caatacgcgt ctcttttggc atatgcaaat cgcgcagtgc cgcgcccacg
    10681 caccatttat cggcactcaa ttgataaaca aactgctccc acggattttc cggatgaata
    10741 tccaggccaa cgcgtgacac cggacgtccc actggcggaa ccaccacttt ggctttttta
    10801 gccgcccacg agagtgatgt tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg
    10861 acattaaaga acagacgtgc attctccagc cccgccatca tcgggaacac ggccaggatg
    10921 attggcaccg cgccgcgtaa tcctacccag ctgataaaca cgcgctcacg cagattgaaa
    10981 ccgcggaagg ggagcaatcc agcaaatacc gaaaggggac gggcgaagaa tatcatccat
    11041 gccgacaaaa ttagcgccgg aatggcaata ggcagcagat cgcttgggtt aaccaatagc
    11101 cccagcacca ggaacatggc gatttgcgcc agccaggcga ggccgtcgaa attttgcagg
    11161 atgccgtagc ggttgcgaat cggccgatta cccagcagaa aaccgcacag ataaacagcc
    11221 agaataccgc tgccttccag cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat
    11281 gccagcaatg gatataatcc ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa
    11341 taaccaccgc caagtccgat gacaatcccg aggccaaatt gctgcagaat atcgaccacg
    11401 aacatccaac tgacactgct ttcatgttgc tggatcatcg caatcagggt aatcgtcaga
    11461 aagaccgcca ttggatcatt actgccggat tcaatttcca gcgtcgagcc aacacgttcg
    11521 ttaagcccct taccacccag cagagaaaag accgctgcag catcggtaga gccgacgata
    11581 gcgccgatta ataagccttc aatcaaatca agattaaaca gccacgccgc catcatgccg
    11641 gttaaaccag aggtgataag cacgcccagc gtcgccagcg acagtgccgg tcctaacgcc
    11701 acacgaaagg aactggcctg agtgcgcatc ccgccgtcga gcaaaataat cgccagtgcc
    11761 aggttactta ccatgtaggc gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg
    11821 actcctgcca gcatgccgat cgccagaaaa ataaccagaa taggaatgcc aagacgggaa
    11881 gaaaatgaac taagtaatat actgctggtg acaaggatgg atcctaagat gaaaaggcta
    11941 attattgttg tggcatccaa cggtcggtta ctcctgatta cgctgtctct tatataaacc
    12001 ctaccatatt agcggcagag acagcgtttt acttagccct gaagcgtgat ttttttacat
    12061 tttaagaaca ggatgaccgc taatcgtcaa ctgagtgcct tcccgggtgt tattcagaat
    12121 ggcatgtgcg cccaacggca gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt
    12181 aatgagtgga atcgacaggc gggaacgcaa aaacgcggac actgactcca ggttgtaacc
    12241 tgcgtcataa tcattgggcg tgctgccgct aaagctaccg agaataatcg ccttctgacg
    12301 tggcaaaatt cccgcatgat agagctgcaa cagcatacgt tcgacgcgga aagggtgctc
    12361 gttaatatct tccagtacca ggataccgtt ctcaattttt ggcatccacg gtgtaccaat
    12421 cagtgaaatc agcatcgcaa gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg
    12481 gccttcccct tgccattcaa tggtgaaggt tttattgcgt aacgccagcc agaaatggtg
    12541 ctcggtaaag gcgttcagtt catccgcacc aaagtttgct accagcatgg ggccgctaaa
    12601 ggtgatgaca ttgccttgcg ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc
    12661 gcaaatgagt aacgggtcat gttgttggcg agccaccagt gcctgccagt caatatctgc
    12721 cagtaaacga ctggcaccgt aaccgccgcg tacagccagc acgatggtgt tgggtgtagt
    12781 cagtctggca agggaattga gatcttccag acgctccgtt tccgtacccg caaaacgctc
    12841 acaacgacgg gcaataacct cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg
    12901 gataccacgc agcgcggcgt gctgtttaat gctgtaaccc gatggggcaa ttaagtgaaa
    12961 cagagacatg gcaattcctt gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt
    13021 gtgccgttgt cacctcaacg gcgattccag gctataagga tagaagaagt gaaattgaga
    13081 tggtttgcct ttttgattgt gttattagcg ggttgttcat caaagcatga ctatacgaac
    13141 ccgccgtgga acgcgaaagt tccggtgcaa cgtgcgatgc agtggatgcc aataagccag
    13201 aaagccggtg cagcctgggg cgtcgatcca caattgatca cggcgattat cgctatcgaa
    13261 tcgggtggta atccgaacgc ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa
    13321 gcttcaacct ccggacgtga tgtttatcgc cgtatgggct ggagtggtga gccgacgacc
    13381 agcgagctga aaaatccaga gcgtaatatt tcaatggggg cggcttacct gaatattctg
    13441 gaaaccggcc cgctggcagg cattgaagat ccgaaggtac tgcaatatgc gctggtggtg
    13501 tcatacgcta acggggcagg tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg
    13561 atcagcaaaa tcaacgattt ggatgctgac gagttcctcg atcacgtagc gcgcaatcat
    13621 cctgcgccgc aggctccgcg ctatatctac aaacttgagc aggcactgga cgcgatgtaa
    13681 atcagtcgcg tactttgtcc gctttctccc gggcttcccg ctcgagagag aaaataatcc
    13741 gttgtaattg ccgctccacc gtcgggctga cgttaagaaa acggaagctc agacggggag
    13801 tggtgatggt ttcattcttg ccatcaatca ctttgcgctc gctgatggag attaactggg
    13861 cgtcaaagtg aaaaacaccc cattgcccca tgttgacttc aatctgagcg aagcgcatgc
    13921 cttcttgtaa ttcggcaggc tttgctgttt ccagtaatgc gcccatgccg cctaacgaca
    13981 aatcatacag gcggaaacgt aacgtactgt tatccgccag tttggtctgg caaaaataag
    14041 gcggatggag tggggcggag atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg
    14101 gaggcggtac ggtaataaat gccggaagct gcaagtattc actctgctgt agttgttcaa
    14161 cagtaaactc gactttcgca ccctgagttt cggcggtaat ggtaatatgc tgcgccttta
    14221 gcacggcgat gttgtcttcg gcttgactgc cgaaatccag caccagttta tccggggtta
    14281 ttgccagtat tttgctgatc agctgcccgc cattccaact gagacgcaaa ggaattgcgg
    14341 ctttgtgcaa atcgcgtaac acgcccagga cggctaacgg attttgtttc aggaactgct
    14401 catggtaatg actcacgcgg aaaaactcct gactgacaaa ctgtctttgg tttatcggtc
    14461 acagttaaca aaacttaata caaatgcgtg aatatttttt acatgttgtt cttaaatcag
    14521 ccgacatacg cccagcatcg atcccgccct tgcctatact tagagcgttg acgtaagcat
    14581 ttcttgcgtc cgattcatcg aaacgagggc ttgaacatgg gaattattgc ctggattatt
    14641 tttggcctga tagccggcat tatcgccaag ctaatcatgc cggggcgtga tggtggtgga
    14701 tttttccaga cctgtattct cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc
    14761 atgtttggca ttggcggctc catcagcggt tttaatttgc acagcttcct ggtggcggtg
    14821 gtgggagcta ttctcgttct gggcttattc cgcctgctgc gaagagaata agattttcat
    14881 aaggcggata gcgatacaga tgccgctatc cgcttttcac atcagaacgt atattcgaca
    14941 cctgctttac gggtgaaaaa aatcaatata gcacttcgat atccgataaa gtcggttccc
    15001 ggtaacaaca acagaatcct tttttaatta ttgtttcgtt gtttttggtg tgattcagaa
    15061 aataaatatt ttagacagta gccagtcagg cgttaaaaat ggggcggata gtgaggcggt
    15121 tttggctgcg cttattgaac atttagaatt gatcaatcct tctggcagat taagccaaaa
    15181 tacccgttcg gcaatgcttc agttacgcga agaggaatgg tctgaattct ttttttggtt
    15241 gctaaattct ctggagtgtc tggattatgt gattattaat ttaactccag aaagcaaaaa
    15301 aacgttgatg agtgaacacc gtaacaatat tcaggtagca atcgatgctt tatataggca
    15361 acgtcggtgt aaatcaccgg gggatgatag tgaaacactt acccgtagaa atgacgctat
    15421 cttcggcaac cacgtatggc aaacatttgc gcaatacttc ccgccaggac ttgaaaaacc
    15481 ggctgtgtag aaaacaccat agctatggtg ttatctgccg acaacgccat ccggagaagc
    15541 cgaatgacgt tgtcggagta agcgctggtt aaggtgtggg ttgtgcctct ttggttgagg
    15601 gttgcgtcgt tgctgactta acggtcggac gcgtcgccgg aacattgtca cacggttgct
    15661 ctttcgggca gatcaaatcc agcattttca gcgtcacgcc attggtccag ccaaagccat
    15721 cctgtaatgg atattcgcca cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac
    15781 ccactgtaac ggtgcccagc catttggcgc atcccattgt tgcccacttt tcaccgacgt
    15841 ggtgttcagg ccgccgggtt gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc
    15901 gcgatctttc gctgccgcat tgacgtacag cgggaacagg gcggccgcgg ttaactgatt
    15961 gcgcacttta tgacttttca ggtcgtaatc ggcataccag ccttgttgat cgttccacag
    16021 gtatttttcg attccttttt gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc
    16081 gttatctcct gcagctttgc tggcgcgggc gaggattttt tccattttaa acatcaggct
    16141 gttcagatcg accggtacga tgctggtggt gcgtaaggta tttaactgct gcgggttgtc
    16201 catccagcgc gagctgaaat cccagccaga cgcagcggca gagcgcaggt cgcggtaaat
    16261 ttcagtggca ggtcgattcg gattgctttt ggcggtggca atatcttcca cccatgactc
    16321 tggtcgtggc gtatcgcgat cgtcccagta gcggttgaga agggtaccat cctgaagttt
    16381 gacaacgcgt ttttcctgtt gtccggcttg caggttttca acaccgtcca tccagtaagc
    16441 atattctttt tgcatttgcg gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc
    16501 cagtaactct accatcaggg caaagaaggg cggttgcgag cggcttaaat agtaactgcg
    16561 gttgccgttg ggaatatgac cgtaagtgtc tatttcatga gcaaaat
//
LOCUS       DAJTYD010000077        16477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTYD010000077 DAJTYD010000000
VERSION     DAJTYD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.077"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(48..2489)
                     /gene="rnr"
                     /locus_tag="OO585_002269"
     CDS             complement(48..2489)
                     /gene="rnr"
                     /locus_tag="OO585_002269"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6629840.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2528..2953)
                     /gene="nsrR"
                     /locus_tag="OO585_002270"
     CDS             complement(2528..2953)
                     /gene="nsrR"
                     /locus_tag="OO585_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6629841.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3158..4456)
                     /gene="purA"
                     /locus_tag="OO585_002271"
     CDS             complement(3158..4456)
                     /gene="purA"
                     /locus_tag="OO585_002271"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6629842.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4560..4757)
                     /locus_tag="OO585_002272"
     CDS             complement(4560..4757)
                     /locus_tag="OO585_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6629843.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4839..5843)
                     /gene="hflC"
                     /locus_tag="OO585_002273"
     CDS             complement(4839..5843)
                     /gene="hflC"
                     /locus_tag="OO585_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6629844.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5846..7105)
                     /gene="hflK"
                     /locus_tag="OO585_002274"
     CDS             complement(5846..7105)
                     /gene="hflK"
                     /locus_tag="OO585_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6629845.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7191..8471)
                     /gene="hflX"
                     /locus_tag="OO585_002275"
     CDS             complement(7191..8471)
                     /gene="hflX"
                     /locus_tag="OO585_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6629846.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETILADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8548..8856)
                     /gene="hfq"
                     /locus_tag="OO585_002276"
     CDS             complement(8548..8856)
                     /gene="hfq"
                     /locus_tag="OO585_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6629847.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8942..9892)
                     /gene="miaA"
                     /locus_tag="OO585_002277"
     CDS             complement(8942..9892)
                     /gene="miaA"
                     /locus_tag="OO585_002277"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6629848.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAITG"
     gene            complement(9885..11732)
                     /gene="mutL"
                     /locus_tag="OO585_002278"
     CDS             complement(9885..11732)
                     /gene="mutL"
                     /locus_tag="OO585_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6629849.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11742..13079)
                     /gene="amiB"
                     /locus_tag="OO585_002279"
     CDS             complement(11742..13079)
                     /gene="amiB"
                     /locus_tag="OO585_002279"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6629850.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13098..13559)
                     /gene="tsaE"
                     /locus_tag="OO585_002280"
     CDS             complement(13098..13559)
                     /gene="tsaE"
                     /locus_tag="OO585_002280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6629851.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13531..15078)
                     /gene="nnr"
                     /locus_tag="OO585_002281"
     CDS             complement(13531..15078)
                     /gene="nnr"
                     /locus_tag="OO585_002281"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6629852.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHSITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWGDVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15077..16216
                     /gene="queG"
                     /locus_tag="OO585_002282"
     CDS             15077..16216
                     /gene="queG"
                     /locus_tag="OO585_002282"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6629853.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGGH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16199..16252)
                     /locus_tag="OO585_002283"
     CDS             complement(16199..16252)
                     /locus_tag="OO585_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629854.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3755 a         4706 c         4120 g         3896 t
ORIGIN      
        1 gcattttgtc agcaatctaa ccctcttctg tttgaagagg gtaatgatca ctctgccact
       61 tttttcttcg ccgcacgctt cgctttggtc gctgcagcta ttttctgcgt tttcgccgat
      121 ggctttttcg cttttctcgc gtctttcttc gctgctttcg gcttcgtttt tttctcaccg
      181 cggaaggcgc tgtctggctc aaagtttacc tttttaccga ccagacgacg cttgccgcct
      241 tttttacctg catcgccttt tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt
      301 gcgcgttcgc tggagatcag gctaaagtcg attttgcgct cgtccatatt aaccgcttcg
      361 acgcgaactt ccacgcgatc gcccagacga taagtctggc cgctggattc acccatcagc
      421 cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccaga
      481 ccatcaatga ataagtcgtc cagacggaca aagaatccaa agccagtgac gctggaaatt
      541 acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagcc
      601 acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccagttgc
      661 agcatttctt ccatcgaata atggtagccg ccggtttcag tggtgttgcc ctgatgcccc
      721 tgctctttcg ccagcagata tttaatggcg cggtgcagcg tcaggtctgg ataacgacga
      781 atcggcgaag taaagtgcgc ataggactgc aatgccaggc caaagtgacc acggttttct
      841 ggatcgtaaa tcgcctgttt catcgagcgc agcagcatgg tttgcagcat ttctgcatca
      901 ggacgatcgg caaccgactc cagcagctcc gcgtagtcac gcggttccgg cttgttaccg
      961 cccggcagtt ccagccccag ctccgccagt actgaacgga aagaggtaat cgcttcggtg
     1021 ctcggcttgt cgtgaatacg gaacaatgct ggttctttgg ctttctcaac gaaacgcgcc
     1081 gccgagatat tcgccagaat catgcactct tcaattaact tgtgtgcgtc gttacgctga
     1141 gtctgttcga tacgctcaat acggcattca gcgttgaaaa taaacttcgc ttcttcgctc
     1201 tcaaatgaga tcccgccacg ttcttcacgg gctttatcca gcactttata gaggttatgc
     1261 aactcttcga gatgcttaac cagcggagcg tactgctcgc gcagatcctg atcgccctgc
     1321 agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag
     1381 aatttgtagc ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg
     1441 cggtctacct gcggattgag cgagcacaga ccgttagaga gcacttccgg cagcataggg
     1501 ataacctgcg aagggaagta caccgacgta ccacgattac gcgcttctct atccagcggc
     1561 gttggcggac gcacatagta gctgacgtcg gcaatcgcga cccataaacg ccagccgccg
     1621 ccgcgttttt tctcgcagta aaccgcatcg tcaaagtcac gggcgtcttc gccatcaatg
     1681 gtgaccagcg gtaaatcgcg caaatcgaca cggcccgctt ttgcttcttc cggcacttct
     1741 tctttcagcc cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg
     1801 gtacgcaaag cgatatcaac cgccatgccg gtgcccatat tgtcgcccag cacttcgacg
     1861 attttaccca ccgctttggt acggcgagtc gggcgttggg tcagttcaac aaccaccaca
     1921 aagcccatcc gcgcgcccat gatctgatcg ggcgggatta agatatcgaa gctcagacgg
     1981 ctatcgtcag gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc aacaatctgg
     2041 ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagca
     2101 cccagcggct gagccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag
     2161 agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca
     2221 ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagtg
     2281 aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc
     2341 tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt
     2401 ttggttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct
     2461 tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag
     2521 attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg
     2581 caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt
     2641 tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg
     2701 gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccggttta cccaggcgaa
     2761 tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta
     2821 ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca
     2881 tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac
     2941 tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga
     3001 taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg
     3061 atttaacact gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt
     3121 agcccggcag gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac gggtcgcgca
     3181 gaatcatagt ttcagtacga tccggaccgg tagagatgat atcgatcggc acaccggtca
     3241 gctcttcaat acgcttgatg tagttcagcg ccgcctgcgg cagcccgcta cgatctttca
     3301 cgccgaaagt ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc
     3361 agtcgtcagc tgccagcgga gtggtagtca cttcgcgacc atccggcata cggtaagcca
     3421 cgcagagttt aacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag
     3481 acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac
     3541 gacgacgacc cgtcgttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt
     3601 catcaaacag ttcggtcggg aaaggacctg cacccacacg agtggagtaa gctttgagga
     3661 tacccagaac gtaatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag
     3721 cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac
     3781 cctgcgcacc ttcaaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag
     3841 aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt
     3901 tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca
     3961 tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac
     4021 cgcgacgtgc cactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg
     4081 ctttcgcgcc acgcgctttc tcacgcgcgt tatccagcgc aacgtgataa tcaaggatca
     4141 gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt
     4201 ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctgg
     4261 ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga
     4321 ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt
     4381 cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga
     4441 cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct
     4501 ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt
     4561 cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg
     4621 ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat
     4681 gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc
     4741 cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac atccctgagg
     4801 atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc
     4861 gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca
     4921 gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta
     4981 ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg catgatgcgg
     5041 ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt
     5101 ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg
     5161 cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttgatacgc
     5221 acatcgacaa cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg
     5281 cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgctgccggg
     5341 gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg
     5401 gtcagacgac cacgggaatc agtgacgata tctttcacgt ccaggcgacc aatttcagaa
     5461 cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgccacca
     5521 cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg
     5581 atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata
     5641 cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca
     5701 taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg
     5761 cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata
     5821 atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg
     5881 cgttggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc
     5941 cactggttgt ggaggaagag gctggcggca aacgcagcag attgctggca ccgttatcgc
     6001 tcttcgccgc aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt
     6061 tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct
     6121 cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag
     6181 caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct
     6241 cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt
     6301 ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg
     6361 cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcaga gtgatgccca
     6421 tgtcatacgg acgaatcgtc tcttccagtt cgcgctgggt atcgctacga atcacggtac
     6481 gaccttccgt cagaatgcgg tccatggtgt atttaccgat aaccccgcgc agggcgctgt
     6541 cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat
     6601 aggtaacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca
     6661 tcacaccgga agcggccagt tcacgcacgg cttccacgtt taccggtttg acttcgtcga
     6721 taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta ccaaagcgtg
     6781 ttaccacgcc gcgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga
     6841 caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg
     6901 atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttgc
     6961 tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc
     7021 catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt
     7081 tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg
     7141 ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg
     7201 taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga
     7261 acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga
     7321 taaaaacgac ttctcagacg cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc
     7381 tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt
     7441 gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa
     7501 tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc
     7561 tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc cgccgcatca
     7621 atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgccgcc
     7681 accagatcgt gcggcagatg gcgaataaac cctacggtat ctgcaagtat ggtttcaccg
     7741 acatccgcga cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc
     7801 gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat
     7861 cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc
     7921 tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg caacaaacga
     7981 cggtcggttt cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt
     8041 tcaaggtggg tccagccacg caccaggcgc gtagccagat ggcgcagctg cgccagctca
     8101 acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg
     8161 gtgcggtcga tgacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc
     8221 agggcatggt caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca
     8281 actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc
     8341 acctgcaatg cttcgacacc ggcggaagaa accagagatt caaactcctg gaggtcttcc
     8401 atatctttgt ctttcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa
     8461 cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc
     8521 cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga
     8581 agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt
     8641 actgtgatga gaaaccggac gagacgggac aacagtagaa atcgcgtgct tgtaaaccat
     8701 ctggctgacc gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg
     8761 cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt
     8821 caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat
     8881 ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca
     8941 ttcagcctgt gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt
     9001 cactgtcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca
     9061 actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa
     9121 ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat
     9181 ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa
     9241 aacgctgctc aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat
     9301 gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg
     9361 aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg
     9421 ctgccgcaac cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg
     9481 ccgcctgttg ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc
     9541 cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg
     9601 ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag
     9661 cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt
     9721 tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca
     9781 actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg
     9841 gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg
     9901 gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca
     9961 agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac
    10021 tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat
    10081 acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg
    10141 cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata
    10201 cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact
    10261 tttagccgca acggaataag cagcggctga gcgcaaactg gcgcttcacc cggggtcagt
    10321 tgtacctgac gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg
    10381 cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg
    10441 ttcgccgcaa gtgcaggttc ctgcggttcc ggcgctttta atttttgcat cggcgcgggc
    10501 gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc
    10561 ggccaagggg cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctacc
    10621 ggctcacgag ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccgga
    10681 atggaacgcg gtgcaggttg gggttcatcg tccagcggta gcggcgtttc cagttgctgt
    10741 tgtagcacgc tcagcacgcc ctgatagata aaatcatgca ccagacgcga ctgatggaaa
    10801 cgcacttcgt gtttggcggg gtgcacgttg acgtccacct gatgcggatc gatctccaga
    10861 tacagcacaa atgccggttg ctgatcggcc cccagtttgt cttcgcaggc ctggcggatc
    10921 gcgtgattga tcaggcgatc gcgcatcatg cgaccgttca cgtagcaata ctgaatttct
    10981 gccagtgcgg gcgtggtgtg atttggatcg gccacccagc cgcgtagcgt gagatcgccg
    11041 tgttgccatt caatcgccag cgcttgttca agaaaagcgg tgccgcaaat cgcgcctaag
    11101 cgccgttctt tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta
    11161 tgcgacaggt tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatctcatca
    11221 atgtggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag
    11281 aacagatcca gcacctccag cgtcgtcccc acaggatgcg ccgccggttt taccgtcacg
    11341 ttcatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa
    11401 gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg
    11461 ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg acgagccagc
    11521 gccagcgcca gctcatcttt tttgataccg cagccgttat cacgaatgcg gataagtttc
    11581 gccccaccgc gttcgatatc aatatcgata cgcgtcgcac ctgcatcgag gctgttttcc
    11641 accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc
    11701 gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga
    11761 tctggcgtcg tcaccgtact ggccgtttgt gccgttgcac cctgcggcgc agattgcatc
    11821 ggatgcgcaa ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg
    11881 taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc
    11941 agtactgacg ggatatccgg cgaacgcaga acgccgagac tcgcgtgttc tggtcggcgt
    12001 ttatgtattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct
    12061 acccgctggg aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata gggatcagac
    12121 tgactgttcg ccagcacatc gcccgcccca cccagcagct ccgaatgttt ctcgtgctgc
    12181 tccagccagc tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg
    12241 ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attagcgttt
    12301 tgcttacgtg ccacatcgct gcgccccatc accgaaataa agtaatcccc gtcacgggtt
    12361 aaaacgcctt taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg
    12421 atggtgacat ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg
    12481 tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc
    12541 ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac
    12601 ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc
    12661 gcaaccacgg gcggcggagg aggcggtggc ggcacatcgg cgttaatcgt aaagacgaca
    12721 gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccattttc ggtaagatcg
    12781 accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga
    12841 ttattgccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc
    12901 gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac
    12961 gttatccgcg cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc
    13021 accggcgtgc acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc
    13081 ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa
    13141 accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc
    13201 gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg
    13261 gcaaaataat cgcggatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa
    13321 tcaaagtgat aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg
    13381 cttttgacat taccctgatg acccagagct tgtaaaaagc cccggctaaa ggtggtctta
    13441 cctgcgccta aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc
    13501 cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg
    13561 gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc
    13621 cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac cgtgcgcgac
    13681 acagcccgca caggcggcat catacggcga cagtttttgc ccaagcaatg cgccaataat
    13741 accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat
    13801 gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc
    13861 gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac
    13921 ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg
    13981 cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg
    14041 aaaattctca actttttgca gtgctttttt cccccactct tgctggccca gaccgggacc
    14101 aatcaccacc acatcgcccc attccaggct ttcggtaaga gagtccatcg tcagttcatg
    14161 caccatcaat tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc gggtcagtac
    14221 tcggactaaa ccagcaccag cacgcagcgc cgcttccccc gtcatacgaa tagcccccgc
    14281 cgtgccgtga tcgccaccga taattaccag ccgcccgtga tcgcctttat gcgaagtcgg
    14341 gcgacgcggt tttagccagt gagaaagttg ttctgctgaa aaccgctgaa ttttcgtctc
    14401 ctgacctgcc agccaactat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc
    14461 ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg agtgatcggc
    14521 gttgatcacc gcgcctggcg tagcgccagt ttcagccagc aggccggaag ggatatcaac
    14581 cgccacaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg
    14641 gggcgcttgc cgcaaaccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg
    14701 ccagacaata ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag
    14761 cgcggcctct tccggcaacg gtttgtcgct ctcctgggca aacaacgtga cctcaatgcc
    14821 gaccgctttg gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca
    14881 cagcaccagc cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc
    14941 gccagcgcga agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc
    15001 tccgcggcgg atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt
    15061 cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa
    15121 acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga
    15181 gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat
    15241 ggcacgtcac ggtatgctgc gcgctcgccc tcatgagtta ttgcccggta cgctgcgcgt
    15301 gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa
    15361 ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg
    15421 caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag
    15481 accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg
    15541 ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg
    15601 cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggtaa
    15661 atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga
    15721 tgctcgccgc tgtatctctt atctcaccat cgaacttgaa ggggcgatcc cggaagagtt
    15781 gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagccta tctgcccgtg
    15841 gaatcgctat tcacaactca ccacagaaga ggatttcagc ccgcgtaagc cgctacacgc
    15901 accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg
    15961 atcggcgatt cgtcgaattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg
    16021 caatgcccct tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgggcaccc
    16081 acttctcgat gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg
    16141 catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt
    16201 accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc
    16261 aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta
    16321 tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta
    16381 cagcctgagg ctgtggataa ctctgtttac aagagtttgt cagagacaac gaaaaccagc
    16441 aataacgcat cgctgtggat attttattga gagaaga
//
LOCUS       DAJTYD010000078        16341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTYD010000078 DAJTYD010000000
VERSION     DAJTYD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.078"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="OO585_002284"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="OO585_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6629855.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="OO585_002285"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="OO585_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6629856.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="OO585_002286"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="OO585_002286"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6629857.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="OO585_002287"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="OO585_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6629858.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3240..4635
                     /gene="ychO"
                     /locus_tag="OO585_002288"
                     /pseudo
     CDS             3240..4635
                     /gene="ychO"
                     /locus_tag="OO585_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
     gene            complement(4636..5286)
                     /gene="narL"
                     /locus_tag="OO585_002289"
     CDS             complement(4636..5286)
                     /gene="narL"
                     /locus_tag="OO585_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6629859.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5279..7075)
                     /gene="narX"
                     /locus_tag="OO585_002290"
     CDS             complement(5279..7075)
                     /gene="narX"
                     /locus_tag="OO585_002290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6629860.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRVRLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7414..8805
                     /gene="narK"
                     /locus_tag="OO585_002291"
     CDS             7414..8805
                     /gene="narK"
                     /locus_tag="OO585_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6629861.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9321..13043
                     /locus_tag="OO585_002292"
     CDS             9321..13043
                     /locus_tag="OO585_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6629862.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13061..14599
                     /gene="narH"
                     /locus_tag="OO585_002293"
     CDS             13061..14599
                     /gene="narH"
                     /locus_tag="OO585_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6629863.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14596..15306
                     /gene="narJ"
                     /locus_tag="OO585_002294"
     CDS             14596..15306
                     /gene="narJ"
                     /locus_tag="OO585_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6629864.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15306..15983
                     /gene="narI"
                     /locus_tag="OO585_002295"
     CDS             15306..15983
                     /gene="narI"
                     /locus_tag="OO585_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6629865.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16166..16297)
                     /locus_tag="OO585_002296"
     ncRNA           complement(16166..16297)
                     /ncRNA_class="other"
                     /locus_tag="OO585_002296"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         3925 a         4087 c         4384 g         3945 t
ORIGIN      
        1 ctcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagtgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcgtcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aaatctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgagcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc gcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catgaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggccagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacactca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt tttcattatt accggaagtt accgacgttt
     3241 tgagccgttt cgttcctcgc attatcccgt tttatttact cttgcttgta gcaggcggta
     3301 cagctaacgc acaatctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg
     3361 atggtctgcc ggatttaggc atggctcccg aaaatcatga tggggaaaaa cactttgctg
     3421 aaattgtgaa agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc
     3481 aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc
     3541 acgtagagtc ctggctatca ccgtggggaa atgccagtgt tgacgtcaaa gtggataacg
     3601 aaggacattt caccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt
     3661 atttcacctg gagccagctt ggtcttactc agcaggataa tgggttggtg agcaatgtgg
     3721 gcgttgggca acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca
     3781 acttgctgga cgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt
     3841 tgcgattatt ggcaaacttt tatcagccgt ttgctgcatg gcatgaacag acagccacgc
     3901 aggaacaacg gatggcgcgc gggtacgacc tgacagctcg gatgcgcatg ccgttctatc
     3961 aacacctcaa taccagtgtc agcctagaaa cagtattttg gtgatcgtgt tgatttgttt
     4021 aactctggta cgggttatca caatcccgtc gcgttgagtc tgggattaaa ttacacccct
     4081 gtgccattag tcactgtgac ggcccagcat aaacagggtg aaagtggcga gaatcaaaat
     4141 aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc tcaaaaaaca actttctgcg
     4201 ggcgaggttg ccgaaagtca gtcgttacgt ggtagtcgct atgacaatcc gcagcgaaat
     4261 aatctaccga ctcttgagta ccgacagcga aaaacgttaa cggtgtttct ggcgacaccg
     4321 ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat tacaaatccg cagtcgttac
     4381 ggtattcggc aactgatttg gcagggcgat acgcagatat taagtttgac gccgggcgca
     4441 caagccaaca gcgcggaggg ctggacgctg atcatgcctg actggcagaa cggggaaggg
     4501 gcaagcaatc actggcgatt gtcggtggtg gtggaatata accaggggca gcgtgtctcc
     4561 tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg cattgtcaaa cgacgaactg
     4621 cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc cataccgctg cttccacgcg
     4681 agacttgagc ttcattttct tcagcatgtg cttgacgtgc acttttactg tgctttcggt
     4741 gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc tgggcaatca gcttgagaat
     4801 atcgcgctcg cgtggggtta actggttaac atcgcgctca gtagtggcac ggttagcgcg
     4861 caagctggcg gccagaacag gcgttaatgc ttcgcttaat accatttcgc cagcagctgc
     4921 ctgatgcaat gctttcagca gatcttccgg ttccatatct tttaacagat aaccatccgc
     4981 gccgcgtttc agtgcggtga ccacatcttc ttcatggtta gagacgctga ataccacaat
     5041 gcgccctgag agggactttt cgcgcagttt atccagcgtt tccagaccgt tcatgccggg
     5101 catattgaga tctaacagga tcagatcggg atcaagagac tccgccagtt caataccctg
     5161 ttcgccatta ctcgcttcgc caaccacggt gatatctggt gccatactga taagctgttt
     5221 tacgccagtt cgcagcatcg ggtgatcgtc gatcagcagg atagtagccg gttcctgatt
     5281 actcatgggt atctccttgg acgtctgtga aagttttttc gggaataaag gtgaccacca
     5341 cttcggtgcc acctgattca cgacggcgga cgcggcaatc gcctcgtaaa ctttgcgcac
     5401 ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat ggcattttca ggcacgccgc
     5461 agccgttatc ttggacggtc agtttgactt gattatcgtt ttgcgccacc gtcaccacga
     5521 cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc ctcacgggca atttgcaaca
     5581 agtggattgc ctgatgtgaa ggcaccagac gaggcggcaa ttgataatcc agcttcaccg
     5641 ggaagccaaa tttggcgctg tactcttcgc aactcgcctc cagcgccgga cgtaatccag
     5701 gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg caactgcgcc caggatgcat
     5761 tcagttcgtt acggatctga cttaacagtt cgcggctgct tgctggcagc gcatcgccct
     5821 gcatctgtaa acaactcacc tgcatcttca tgcaagagag agattgggca atagaatcat
     5881 gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat caactgttgc tgacgttcct
     5941 gatggcgatc cagcgccagc gtggcggtga gttgttcaac cagggtatcc accagttgtt
     6001 gttgatcatg gctaagatga cgcccctgcg gcagggtcgc cagcaaaata ccgtactgcg
     6061 tatgagagtc agccagccgc cacttcaggg ttgtgccgcg atcaccaacg ggtaatacgc
     6121 cgcgcgggca gagctggcag cctttatcat cacaagtcat atctggctgg caggtaaact
     6181 cctgatgatt ctcttcatca tcagtgtcat acacccgcag ttcgatatca cgtagcaggg
     6241 ttaaattctg taagccgttg agtacaggtg acaggcgttc acacagcggg gcgcgggaat
     6301 gcaaacggcg gttagcctgc cataaaaaag agaggatctg atttttatgc tccagcccgg
     6361 cggttttctc ctgaacccgc tgctcaagta cggcataact ttcggccagt tctgcagaca
     6421 tattgttcaa tgcagttcca agcatcgcca tttcgttgcg cccgctgatg ttcgcgcgtt
     6481 gggtaaaatc gcgatgactg acggcactcg ccattgccag cagttgccgc cacggttgta
     6541 gcagtcgcac ccgcaaccag ataatagtga acaccagtaa aagtgccata aataccgcca
     6601 ttacccgatg gaccagtacc actgtctcaa tgcgcatttc cgtggtgcgg tcaaaaccag
     6661 ataccagctg atcaagcccg gcaacaaact ggctgacatc cgctgacacc gtttcgcggt
     6721 tttgtgcacg catcagtgca gggatcagtt cattacgcca gtaatcttgt aaaccctgta
     6781 attgcgccag ttgttcgtct cgttctgctg ctcgagttaa ctcggcgcta aatgccgttt
     6841 gttccatctc tttaattaag ggcttgtctt tctcacttaa tggcactgcc gccaacagac
     6901 ggtaactttg catgcgcagc gatcccgctt tgttgatcgc atgggcgctg ccctgaacgc
     6961 cttgcaccag ccagccagaa accgccatcc ctgccagtcc aatagcagta gaaagcaaca
     7021 caataagcgc aacctgatta accagggtga gcggagagag acaacgttta agcatgtaaa
     7081 cctcttcctt caggctttaa atgagcaata accttaatga atgtgacgat acattctgga
     7141 atggcagtat tctcggctat tggctgaagt atacccatac ccggaaagag ttactcctta
     7201 tttgccgtgt ggttagtcgc tttacatcgg taagggtagg gattttacag caccgtgaaa
     7261 aatctcataa tttttatgaa atcactgtac tcactatggg taatgataaa tatcaatgat
     7321 agataaagtt atcttatcgt ttgatttaca tcaaattgcc tttagctaca gacactaagg
     7381 tggcagacat cgaaacgagt atcagaggtg tctatgagtc actcatccgc ccccgaaagg
     7441 gctactggag ctgtcattac agattggcga ccggaagatc ctgcgttctg gcaacaacgc
     7501 ggtcaacgta ttgccagccg taacctgtgg atttccgttc cctgtctgct gctggcgttt
     7561 tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac cgaaagtcgg ttttaatttt
     7621 acgaccgatc agctatttat gttgactgcg ctgccttcgg tttctggcgc gttattacgt
     7681 gttccatact cctttatggt tcctatcttc ggtggtcgtc gctggacggc gttcagcacc
     7741 ggtattctga ttattccttg cgtctggctg ggttttgccg tgcaggatac ctccacgcct
     7801 tatagcgtct tcatcatcat ctctctgcta tgcggctttg ctggcgcgaa cttcgcatcc
     7861 agtatggcaa acatcagctt cttctttccg aaacagaagc agggtggcgc gctgggtctg
     7921 aatggtggtc tgggaaacat gggcgtcagc gtcatgcagt tggttgctcc gctggtggta
     7981 tcactgtcga ttttcgcagt atttggtagc cagggcgtca aacagccgga tgggactgag
     8041 ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc ttgccatctt caccattgcg
     8101 gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct ccatcaagga gcagttgccg
     8161 gtactcaaac ggggtcatct gtggattatg agcctgctgt atctggcaac cttcggctcc
     8221 ttcatcggct tctccgcggg ctttgcaatg ctgtcaaaaa cgcagttccc ggatgttcag
     8281 attctgcaat acgctttctt cgggccgttt attggtgcgc tggcgcgttc tgcaggtggt
     8341 gcattatctg accgtctggg cggaactcgt gtcacgctgg tgaactttat tctgatggcg
     8401 attttcagcg gcctgctgtt cctgacctta ccgactgacg ggcagggcgg aagcttcatg
     8461 gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg ggctgggtag tggttccact
     8521 ttccagatga tttcagtgat cttccgtaaa ctgacaatgg atcgcgtgaa agcagaaggg
     8581 ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca cggcggcggc gctgggtttc
     8641 atctctgcga ttggcgcgat tggtggcttc tttatcccga aagcgtttgg tagctcgctg
     8701 gcattaacgg gttcgccagt cggcgcaatg aaggtatttt tgattttcta tatcgcctgc
     8761 gtagtgatta cctgggcggt atatggtcgg cattctaaaa aataaaccgt tactcgtcat
     8821 actccgggtt acatgtgctg cggctgcgtt cattcacccc agtcacttac tttagtaagc
     8881 tcctgggatt cattcacttg ccgccttcct gtaaaccgaa ttatatagag taaaatattt
     8941 gattatcctt tgcgcggcat gatgtcgcgc tttttttaag cgttatttag ttacaacata
     9001 ctaatgttat atggtttatt tcgccggatt tcattaagag ccattaatat gttacccatg
     9061 gggaatactc cttaataccc atctgcataa aaatcttaat agtttaaata actacaggta
     9121 taaaacgtct taatttacag tctgttatgt ggtggctgtt gattatccta aaggggtatc
     9181 ttaggaattt actttatttt tcatccccat cactcttgat cgttatcaat tcccacgctg
     9241 tttcagagcg ttaccttgcc cttaaacatt agcaatgtcg atttatcaga gggccgacag
     9301 gctcccacag gagaaaaccg atgagtaaat tcctggaccg gtttcgctac ttcaaacaga
     9361 agggtgaaac ctttgccgat gggcatggcc agcttctcaa taccaaccgt gactgggagg
     9421 atggatatcg ccagcgttgg cagcatgaca aaatcgtccg ctctacccac ggggtaaact
     9481 gcaccggctc ctgcagctgg aaaatctacg tcaaaaacgg tctggtcacc tgggaaaccc
     9541 agcagactga ctatccgcgt acccgtccgg atctgccaaa ccatgaacct cgcggctgcc
     9601 cgcgcggtgc cagctactcc tggtatcttt acagtgccaa ccgcctgaaa tacccgatga
     9661 tgcgcaaacg cctgatgaaa atgtggcgtg aagcgaaggc gctgcatagc gatccggttg
     9721 aggcatgggc ttctatcatt gaagacgccg ataaagcgaa aagctttaag caggcgcgtg
     9781 gacgcggtgg atttgttcgt tcttcctggc aggaggtgaa cgaactgatc gccgcatcta
     9841 acgtttacac catcaaaaac tacggcccgg accgtgttgc tggtttctcg ccaattccgg
     9901 caatgtcgat ggtttcttac gcatcgggtg cacgctatct ctcgctgatt ggcggtactt
     9961 gcttaagctt ctacgactgg tactgcgacc tgcctcctgc gtctccgcaa acctggggcg
    10021 agcaaactga cgtaccggaa tctgctgact ggtataactc cagctacatc atcgcctggg
    10081 gttcaaacgt gccgcagacg cgtaccccgg atgctcactt ctttactgaa gtgcgttaca
    10141 aaggcaccaa aactgttgcc gtcacaccag actacgctga aatcgccaaa ctgtgcgatc
    10201 tgtggctggc accgaaacag ggcaccgatg cggcaatggc gctggcgatg ggccacgtaa
    10261 tgctgcgtga attccacctc gacaacccaa gccagtattt caccgactat gtgcgtcgct
    10321 acaccgacat gccgatgctg gtgatgctgg aagaacgcga cggttactac gctgcaggtc
    10381 gtatgctgcg cgctgctgat ctggttgatg cgctgggcca ggaaaacaat ccggaatgga
    10441 aaactgtcgc ctttaatacc aatggcgaaa tggttgcgcc gaacggttct attggcttcc
    10501 gctggggcga gaagggcaaa tggaatcttg agcagcgcga cggcaaaact ggcgaagaaa
    10561 ccgagctgca actgagcctg ctgggtagcc aagatgagat cgctgaggta ggcttcccgt
    10621 actttggtgg cgacggcacg gaacacttca acaaagtgga actggaaaac gtgctgctgc
    10681 acaaactgcc ggtgaaacgc ctgcaactgg ctgatggcag caccgccctg gtgaccaccg
    10741 tttatgatct gacgctggca aactacggtc tggaacgtgg cctgaacgac gttaactgtg
    10801 caaccagcta tgacgatgtg aaagcttata ccccggcctg ggccgagcag attaccggcg
    10861 tttctcgcag ccagattatt cgcatcgccc gtgaatttgc cgataacgct gataaaacgc
    10921 acggtcgttc gatgattatc gtcggtgcgg ggctgaacca ctggtatcac ctcgatatga
    10981 actatcgtgg tctgatcaac atgctgattt tctgcggctg tgtcggtcag agcgggggcg
    11041 gctgggcgca ctatgtaggt caggaaaaac tgcgtccgca aaccggctgg cagccgctgg
    11101 cgtttgccct tgactggcag cgtccggcgc gtcacatgaa cagcacttct tatttctata
    11161 accactccag ccagtggcgt tatgaaaccg tcacggcgga agagttgctg tcaccgatgg
    11221 cggacaaatc ccgctatacc ggacacttga tcgactttaa cgtccgtgcg gaacgcatgg
    11281 gctggctgcc gtctgcaccg cagttaggca ctaacccgct gactatcgct ggcgaagcgg
    11341 aaaaagccgg gatgaatccg gtggactata cggtgaaatc cctgaaagag ggttccatcc
    11401 gttttgcggc agaacaacca gaaaacggta aaaaccaccc gcgcaacctg ttcatctggc
    11461 gttctaacct gctcggttct tccggtaaag gtcatgagtt tatgctcaag tacctgctgg
    11521 ggacggagca cggtatccag ggtaaagatc tggggcaaca gggcggcgtg aagccggaag
    11581 aagtggactg gcaggacaat ggtctggaag gcaagctgga tctggtggtt acgctggact
    11641 tccgtctgtc gagcacctgt ctctattccg acatcatttt gccgacggcg acctggtacg
    11701 aaaaagacga catgaatact tcggatatgc atccgtttat tcacccgctg tctgcggcgg
    11761 tcgatccggc ctgggaagcg aaaagcgact gggaaatcta caaagccatc gcgaagaaat
    11821 tctccgaagt gtgcgtcggc catctgggta aagaaaccga catcgtcacg ctgcctatcc
    11881 agcatgactc tgccgctgaa ctggcgcagc cgctggatgt gaaagactgg aaaaaaggcg
    11941 agtgcgacct gatcccaggt aaaaccgcgc cacacattat ggtcgtagag cgcgcttatc
    12001 cggcgactta cgaacgcttt acctctatcg gcccgctgat ggagaaaatc ggtaatggcg
    12061 gtaaagggat tgcctggaac acccagagcg agatggatct gctgcgtaag ctcaactaca
    12121 ccaaagcgga aggtccggcg aaaggccagc cgatgctgaa caccgcaatt gatgcggcag
    12181 agatgatcct gacactggca ccggaaacca acggtcaggt agccgtgaaa gcctgggctg
    12241 ccctgagcga atttaccggt cgtgaccata cgcatctggc gctgaataaa gaagacgaga
    12301 agatccgctt ccgcgatatt caggcacagc cgcgcaaaat tatctccagc ccgacctggt
    12361 ctggtctgga agatgaacac gtttcttaca acgccggtta caccaacgtt cacgagctga
    12421 tcccatggcg tacgctctct ggtcgtcagc aactgtatca ggatcaccag tggatgcgtg
    12481 atttcggtga aagcctgctg gtttatcgtc cgccgatcga cacccgttcg gtgaaagaag
    12541 tgataggcca gaaatccaac ggcaacccgg aaaaagcgct caacttcctg acgccgcacc
    12601 agaagtgggg gatccactcc acctacagcg acaaccagtt gatgctgacc ttaggtcgcg
    12661 gtggcccggt ggtctggctg agcgaagccg atgccaaaga gctgggcatt gccgataacg
    12721 actggattga agtttacaac agcaacggtg cgctgactgc ccgtgcggtt gtcagccagc
    12781 gtgttccggc agggatgacc atgatgtacc acgcgcagga acgtatcgtt aacctgccgg
    12841 gttcggaaat tactggccag cgtgggggga tccacaactc ggtcacccgt atcacgccga
    12901 aaccgacgca tatgattggt ggctatgccc atctggcata cggctttaac tactacggca
    12961 ccgtaggttc taaccgcgat gagtttgttg tagtgcgtaa gatgaagaac attgactggt
    13021 tagatggcga aggcaatgac taggtacagg agagcgtaaa atgaaaattc gttcacaagt
    13081 cggcatggtg ctgaatctcg ataagtgcat cggctgccac acctgttcag ttacctgtaa
    13141 aaacgtctgg accagccgtg aaggcgtgga gtacgcgtgg ttcaacaacg tggaaaccaa
    13201 gccgggtcag ggcttcccga ctgactggga aaaccaggaa aaatacaaag gcggctggat
    13261 ccgtaaaatc aacggcaaac tgcagccgcg catgggtaac cgtgccatgt tgctgggtaa
    13321 aatcttcgct aacccgcatc tgccggggat cgacgattat tacgagccgt tcgattttga
    13381 ctatcagaac ctgcataccg cgccggaagg cagcaaatcg cagccgattg cccgtccccg
    13441 ttcgctgatt accggggaac ggatggcgaa aatcgaaaaa gggccgaact gggaagatga
    13501 cctgggtggt gagtttgaca aactggcgaa agacaagaac ttcgacaaca tccagaaggc
    13561 gatgtatagc cagttcgaaa acacctttat gatgtatttg ccgcgcctgt gcgaacactg
    13621 cctgaacccg gcatgtgtgg cgacctgccc gagcggtgcg atttacaagc gtgaagaaga
    13681 tggcatcgtc ctgatcgacc aggataaatg ccgtggctgg cgtatgtgca tcaccggatg
    13741 cccgtacaaa aaaatctact tcaactggaa gagcggtaag tctgagaagt gcatcttctg
    13801 ctatccgcgt attgaagcgg gtcagccgac cgtgtgctca gaaacctgtg tcggtcgtat
    13861 ccgttatctt ggcgtgctgc tgtacgatgc cgacgcgatt gaacgtgcag ccagcaccga
    13921 gaacgagaaa gatctttacc agcgtcagct ggaggtgttc ctcgatccga acgatccgaa
    13981 agtcatcgag caggcgatta aagacggtat tccgctgagc gttattgaag ccgcacagca
    14041 gtcgccggtt tataaaatgg caatggaatg gaaactggcg ctgccgctgc atccagaata
    14101 tcgcacactg ccgatggtct ggtacgtgcc gcctctgtct ccgattcagt ctgcagcaga
    14161 cgcgggtgag ctgggtagca acggcattct gccagacgtc gaaagcttgc gtattccggt
    14221 acagtatctg gcgaatctgc tgaccgccgg tgataccaaa ccggtactgc gcgcactgaa
    14281 acgtatgctg gcgatgcgtc attacaaacg tgctgaaacc gttgacggta aagttgatac
    14341 ccgtgcgctg gaagaggtcg gtctgaccga agcccaggca caggagatgt accgttatct
    14401 ggctattgct aactacgaag atcgctttgt ggtgccgagt agtcatcgtg aactggcacg
    14461 ggaagccttc ccggagaaaa atggctgcgg ctttaccttt ggtgatggct gccacggctc
    14521 agataccaaa ttcaatctgt tcaacagccg tcgtatcgat gccatcgatg tgaccagcaa
    14581 aacggagccg catccatgat cgaactcgtg attgtatcgc gtctccttga atatccggat
    14641 gctgccttat ggcagcatca acaagagatg tttgaggtga ttgctgcgtc gaaaaatctg
    14701 tcgaaagaag atgcccatgc gctgggcatt ttcctgcgcg atttaacggc gatggatccg
    14761 ctcgatgccc aggcccagta cagcgaactg ttcgaccgtg gccgcgccac gtcactgttg
    14821 ctatttgaac atgttcacgg tgaatcccgt gaccgcggcc aggcgatggt ggacctgctg
    14881 gcgcagtacg agcagcacgg cttgcagtta aacagccgcg aattaccgga ccatctgccg
    14941 ctgtatctgg agtacctgtc gcagctgccg caaagcgaag ccgtggaagg tttgaaagat
    15001 atcgcgccga ttctggcatt gctgagcgcg cgtctgcaac agcgtgaaag ccgttatgcc
    15061 gtgatgtttg atctgctgct gaaattggcc aataccgcta tcgacagcga caaagtggcg
    15121 gaaaaaattg ccgacgaagc gcgcgatgat acgccgcagg cgctggatgc tgtctgggaa
    15181 gaagagcagg ttaaattctt tgctgacaaa ggctgcggtg attcagcaat cactgctcat
    15241 cagcgtcgct ttgccggtgc cgtcgcgccg caatatctga atatcaccac cggaggacag
    15301 cactaatgca attcctgaat atgttcttct ttgatatcta cccgtacata gccggggcgg
    15361 tcttcctgat tggtagctgg ctgcgttatg actacgggca gtacacctgg cgcgcggcgt
    15421 ccagccagat gctggatcgc aaagggatga acctggcgtc gaacctgttc catatcggta
    15481 ttctggggat ttttgctggt cacttcttcg gtatgctgac gccgcactgg atgtatgaag
    15541 cctggctgcc gattgaagtg aaacagaaaa tggcgatgtt tgctggtggt gccagcggcg
    15601 tgctgtgtct gattggcggc gtgctgttgc tgaagcgtcg tctgttcagc ccacgcgtgc
    15661 gtgcaaccac taccggagcg gatatcctga tcctgtcgct gctcgttatc cagtgcgcgc
    15721 tgggcctgtt gaccattccg ttctccgctc agcatatgga cggtagcgag atgatgaaac
    15781 tggttggctg ggcgcagtcg gtggtgacct tccacggtgg cgcttctcaa cacctcgatg
    15841 gtgtggcgtt tatcttccgt ctgcacctgg tgctggggat gacgttattc ctgctgttcc
    15901 cgttctcgcg tctggtacac atctggagcg taccggtgga gtatctgaca cgtaagtacc
    15961 agctggtgcg cgctcgtcac taaacgaatt ttagttcaca tagaccctgc ttcggcgggg
    16021 ttttttatgg gcacggtgcg gggtgagttg tcggatgcgc ttcgcttatc cgacctacag
    16081 gggaggatat tgtaggcccg gtaaacgtgg tgccatcggg caaaattagc tcggagtaac
    16141 aggttttgat tattcaagga ggcgatggtg gtgggggaag gattactcag cgctgcgcgc
    16201 ttcgcccttc gggtcgttgc ctgcggcaac gctctctcgc tggcgctcaa gtcgaacctt
    16261 ggtcgaagct tctcatcctt ccccgcttgg gcagaatatt taattgcgga ttcgtttgag
    16321 agtttacgga cttttgaaaa g
//
LOCUS       DAJTYD010000079        16015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTYD010000079 DAJTYD010000000
VERSION     DAJTYD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.079"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..943)
                     /gene="gltA"
                     /locus_tag="OO585_002297"
     CDS             complement(<1..943)
                     /gene="gltA"
                     /locus_tag="OO585_002297"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6629866.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDP"
     gene            complement(1149..1256)
                     /locus_tag="OO585_002298"
     CDS             complement(1149..1256)
                     /locus_tag="OO585_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629867.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            1637..2041
                     /gene="sdhC"
                     /locus_tag="OO585_002299"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             1637..2041
                     /gene="sdhC"
                     /locus_tag="OO585_002299"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6629868.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2035..2382
                     /gene="sdhD"
                     /locus_tag="OO585_002300"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2035..2382
                     /gene="sdhD"
                     /locus_tag="OO585_002300"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6629869.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2382..4148
                     /gene="sdhA"
                     /locus_tag="OO585_002301"
     CDS             2382..4148
                     /gene="sdhA"
                     /locus_tag="OO585_002301"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6629870.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4164..4880
                     /gene="sdhB"
                     /locus_tag="OO585_002302"
     CDS             4164..4880
                     /gene="sdhB"
                     /locus_tag="OO585_002302"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6629871.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5181..7982
                     /gene="sucA"
                     /locus_tag="OO585_002303"
     CDS             5181..7982
                     /gene="sucA"
                     /locus_tag="OO585_002303"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6629872.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            7997..9214
                     /gene="odhB"
                     /locus_tag="OO585_002304"
     CDS             7997..9214
                     /gene="odhB"
                     /locus_tag="OO585_002304"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6629873.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9490..10656
                     /gene="sucC"
                     /locus_tag="OO585_002305"
     CDS             9490..10656
                     /gene="sucC"
                     /locus_tag="OO585_002305"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6629874.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10656..11525
                     /gene="sucD"
                     /locus_tag="OO585_002306"
     CDS             10656..11525
                     /gene="sucD"
                     /locus_tag="OO585_002306"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6629875.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11629..12351)
                     /gene="mngR"
                     /locus_tag="OO585_002307"
     CDS             complement(11629..12351)
                     /gene="mngR"
                     /locus_tag="OO585_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6629876.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12520..14436
                     /gene="mngA"
                     /locus_tag="OO585_002308"
     CDS             12520..14436
                     /gene="mngA"
                     /locus_tag="OO585_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6629877.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14454..>16015
                     /locus_tag="OO585_002309"
     CDS             14454..>16015
                     /locus_tag="OO585_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6629878.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
BASE COUNT         3835 a         4028 c         4387 g         3765 t
ORIGIN      
        1 gcgggtcgta atttttgtac acgcggtgac cgaagcccat caggcggaaa gaatcatttt
       61 tatctttcgc acgacgaaca aattccggaa tgtgtttaac ggagctaatt tcttccagca
      121 ttttcagcgc cgcttcgtta gcaccaccgt gcgcaggtcc ccacagtgaa gcaatacctg
      181 ctgcgataca ggcaaacggg ttcgcacccg aagagccagc ggtacgcacg gtggaggtag
      241 aggcgttctg ttcatggtca gcgtgcagga tcagaatacg gtccatagca cgttccagaa
      301 tcggattaac ttcatacggt tcgcacggcg tggagaacat catattcagg aagttaccgg
      361 cataggagag atcgttgcgc ggataaacaa atggctgacc aatggaatac ttgtaacaca
      421 tcgcggccat ggtcggcatt ttcgacagca ggcggaacgc ggcaatttca cgatgacgag
      481 gattgttaac atccagcgag tcgtgataga acgccgccag cgcgccggta ataccacaca
      541 tgactgccat tggatgtgag tcgcgacgga aagcgtggaa cagacgggta atctgctcgt
      601 ggatcatggt atgacgggtc accgtagttt taaattcgtc atactgttcc tgagtcggtt
      661 tttcaccatt cagcaggatg taacaaactt ccaggtagtt agaatcggtc gccagctgat
      721 cgatcgggaa accgcggtgc agcaaaatac cttcatcacc atcaataaaa gtaattttag
      781 attcgcagga tgcggttgaa gtgaagcctg ggtcaaaggt gaacacacct tttgaaccga
      841 gagtacggat atcaataaca tcttgaccca gcgtgccttt cagcacatcc agttcaacag
      901 ctgtgtcccc gttgagggtg agttttgctt ttgtatcagc catttaaggt ctccttagcg
      961 ccttattgcg taagactgcc ggaacttaaa tttgccttcg cacatcaacc tggctttacc
     1021 cgtttgttat ttggctcgcc gctctgtgaa agaggggaaa acctgggtac agagctctgg
     1081 gcgcttgcag gtaaaggatc cattgatgac gaataaatgg cgaatcaagt acttagcaat
     1141 ccgaattatt aaacttgtct accactaata actgtcccga atgaattggt caatacttcc
     1201 acactgttac ataagttaat cttaggtgaa ataccgactt cataactttt acgcattata
     1261 tgcttttcct ggtaatgttt gtaacaactt tgttgagtga ttgtcaaatt agatgattaa
     1321 aaattaaata aatgttgttg tcgtgacctg gatcactgtt caggataaaa cccgacaaac
     1381 tatatgtagg ttaattgtaa tgattttgtg aacagcctat actgccgcta ggtctccgga
     1441 acaccctgca atcccgagcc acccagcgtt gtaacgtgtc gttttcgcat ctggaagcag
     1501 tgttttgcat gacgcgcagt tatagaaagg acgctgtctg acccgcaagc agaccggagg
     1561 aaggaaatcc cgacgtctcc aggtaacaga aagttaacct ctgtgcccgt agtccccagg
     1621 gaataataag aacagcatgt gggcgttatt catgataaga aatgtgaaaa aacaaagacc
     1681 tgttaatctg gacctacaga ccatccggtt ccccatcacg gcgatagcgt ccattctcca
     1741 tcgcgtttcc ggtgtgatca cctttgttgc agtgggcatc ctgctgtggc ttctgggtac
     1801 cagcctctct tcccctgaag gtttcgagca agcttccgcg attatgggca gcttcttcgt
     1861 caaatttatc atgtggggca tccttaccgc tctggcgtat cacgtcgtcg taggtattcg
     1921 ccacatgatg atggattttg gctatctgga agaaacattc gaagcgggta aacgctccgc
     1981 caaaatctcc tttgttatta ctgtcgtgct ttcacttctc gcaggagtcc tcgtatggta
     2041 agcaacgcct ccgcattagg acgcaatggc gtacatgatt tcatcctcgt tcgcgctacc
     2101 gctatcgtcc tgacgctcta catcatttat atggtcggtt ttttcgccac cagtggcgaa
     2161 ctgacatatg aagtctggat cggtttcttc gcctctgcgt tcaccaaagt gttcaccctg
     2221 ctggcgctgt tttctatctt gattcatgcc tggatcggca tgtggcaggt gttgaccgac
     2281 tacgttaaac cgctggcatt gcgcctgatg ctgcaactgg tgattgtcgt tgcactggtg
     2341 gtttacgtga tttatggatt cgttgtggtg tggggtgtgt gatgaaattg ccagtcagag
     2401 aatttgatgc agttgtgatt ggtgccggtg gcgcaggtat gcgcgcggcg ctgcaaattt
     2461 cccagagcgg ccagacctgt gcgctgctct ctaaagtctt cccgacccgt tcccataccg
     2521 tttctgcgca aggcggcatt accgttgcgc tgggtaatac ccatgaagat aactgggaat
     2581 ggcatatgta cgacaccgta aaagggtcgg actatatcgg tgaccaggac gcgattgaat
     2641 atatgtgtaa aaccgggccg gaagcgattc tggaactcga acacatgggc ctgccgttct
     2701 cgcgtcttga tgatggtcgt atctatcaac gtccgtttgg cggtcagtcg aaaaacttcg
     2761 gcggcgagca ggcggcacgc actgcggcgg cggctgaccg taccggtcac gcactgttgc
     2821 acacgcttta tcagcagaac ctgaaaaacc acaccaccat tttctccgag tggtatgcgc
     2881 tggatctggt gaagaaccag gatggcgcgg tggtgggttg tactgcactg tgcatcgaaa
     2941 ctggtgaagt ggtttatttc aaagcccgcg ctaccgtgct ggcgactggc ggggcagggc
     3001 gtatttatca gtccaccact aacgcccaca ttaacactgg cgacggcgtc ggcatggcta
     3061 tccgcgcagg cgtaccggtg caggatatgg aaatgtggca gttccacccg accggtattg
     3121 ccggtgcggg cgtactggtc accgaaggtt gccgtggtga aggcggttat ctgctgaaca
     3181 aacatggcga acgctttatg gagcgttatg cgccgaacgc caaagacctg gcgggccgtg
     3241 atgtggtggc gcgttccatc atgatcgaaa tccgtgaagg tcgcggctgt gatggtccgt
     3301 gggggccaca cgcgaaactg aaactcgacc acctgggtaa agaagttctc gaatcccgtc
     3361 tgccgggcat cctggaactc tcccgtacct tcgctcacgt cgatccggtg aaagagccga
     3421 tcccggttat cccaacctgt cattacatga tgggcggtat tccgaccaaa gtgaccggtc
     3481 aggcgctgac tgtgaatgag aaaggcgaag atgtggttgt tccgggactg tttgccgttg
     3541 gtgaaatcgc ttgtgtatcg gtacacggcg ctaaccgtct gggcggcaac tcgctgctgg
     3601 acctggtggt ctttggtcgt gcggcaggtc tgcatctgca agagtctatc gccgagcagg
     3661 gcgcattgcg cgatgccagc gagtctgatg ttgaagcatc tctggatcgt ctgaaccgct
     3721 ggaacaataa tcgtaacggt gaagatccgg tggcgattcg taaagcgctg caagaatgta
     3781 tgcagcataa cttctcggtc ttccgtgaag gtgatgcgat ggcgaaaggg cttgagcagt
     3841 tgaaagttat ccgcgagcgt ctgaaaaatg cccgtctgga tgacacttcc agcgagttca
     3901 acacccagcg cgttgagtgc ctggaactgg ataacctgat ggaaacggcg tatgcaacgg
     3961 ctgtttctgc caacttccgt accgaaagcc gtggcgcgca tagccgcttc gacttcccgg
     4021 atcgtgatga tgaaaactgg ctgtgccact ccctgtatct gccagagtcg gaatccatga
     4081 cgcgccgaag cgtcaacatg gaaccgaaac tgcgcccggc attcccgccg aagattcgta
     4141 cttactaatg cggagacagg aaaatgagac tcgagttttc aatttatcgc tataacccgg
     4201 atgttgatga tgctccgcgt atgcaggatt acaccctgga agcggaagaa ggtcgcgaca
     4261 tgatgctgct ggatgcgctt atccagctga aagagaaaga tcccagcctg tcgttccgcc
     4321 gctcctgtcg tgaaggtgtg tgcggttccg acggtctgaa catgaacggc aagaatggtc
     4381 tggcctgtat taccccgatt tcggcactca accagccggg caagaagatt gtgattcgcc
     4441 cgctgccagg tttaccggtg atccgcgatt tggtggtaga catgggacaa ttctatgcgc
     4501 aatatgagaa aattaagcct tacctgttga ataatggaca aaatccgcca gctcgcgagc
     4561 atttacagat gccagagcag cgcgaaaaac tcgacgggct gtatgaatgt attctctgcg
     4621 catgttgttc aacctcttgt ccgtctttct ggtggaatcc cgataagttt atcggcccgg
     4681 caggcttgtt agcggcatat cgtttcctga tcgatagccg tgataccgag actgacagcc
     4741 gcctcgacgg tttgagcgat gcattcagcg tattccgctg tcacagcatc atgaactgcg
     4801 tcagtgtatg tccgaagggg ctgaacccga cgcgcgccat cggccatatc aagtcgatgt
     4861 tgttgcaacg taatgcgtaa accgtaggcc tgataagacg cgcaagcgtc gcatcaggca
     4921 accagtgccg gatgcggcgt gaacgcctta tccggcctac aagtcattac ccgtaggcct
     4981 gataagcgca gcgcatcagg cgtaacaaag aaatgcagga aatctttaaa aactgcccct
     5041 gacactaaga cagtttttaa aggttccttc gcgagccact acgtagacaa gagctcgcaa
     5101 gtgaaccccg gcacgcacat cactgtgcgt ggtagtatcc acggcgaagt aagcataaaa
     5161 aagatgctta agggatcacg atgcagaaca gcgctttgaa agcctggttg gactcttctt
     5221 acctctctgg cgcaaaccag agctggatag aacagctcta tgaagacttc ttaaccgatc
     5281 ctgactcggt tgacgctaac tggcgttcga cgttccagca gttacctggt acgggagtca
     5341 aaccggatca attccactct caaacgcgtg aatatttccg ccgcctggcg aaagacgctt
     5401 cacgttactc ttcaacgatc tccgaccctg acaccaatgt gaagcaggtt aaagtcctgc
     5461 agctcattaa cgcataccgc ttccgtggtc accagcatgc gaatctcgat ccgctgggac
     5521 tgtggcagca agataaagtg gccgatctgg atccgtcttt ccacgatctg accgaagcag
     5581 acttccagga aaccttcaac gtcggttcat ttgccagcgg caaagaaacc atgaaactcg
     5641 gcgagctgct ggaagccctc aagcaaacct actgcggccc gattggtgcc gagtatatgc
     5701 acatcaccag caccgaagaa aaacgctgga tccaacagcg tatcgagtct ggtcgcgcga
     5761 ctttcaatag cgaagagaaa aaacgcttct taagcgaact gaccgccgct gaaggccttg
     5821 aacgttacct cggcgcaaaa ttccctggcg caaaacgctt ctcgctggaa ggcggtgacg
     5881 cgttaatccc gatgcttaaa gagatgatcc gccacgctgg caacagcggc acccgcgaag
     5941 tggttctcgg gatggcgcac cgtggtcgtc tgaacgtgct ggtgaacgtg ctgggtaaaa
     6001 aaccgcaaga cttgttcgac gagttcgccg gtaaacataa agaacacctc ggcacgggtg
     6061 acgtgaaata ccacatgggc ttctcgtctg acttccagac cgatggcggc ctggtgcacc
     6121 tggcgctggc gtttaacccg tctcaccttg agattgtaag cccggtagtt atcggttctg
     6181 ttcgtgcccg tctggacaga cttgatgagc cgagcagcaa caaagtgctg ccaatcacca
     6241 tccacggtga cgccgcagtg accgggcagg gcgtggttca ggaaaccctg aacatgtcga
     6301 aagcgcgtgg ttatgaagtt ggcggtacgg tacgtatcgt tatcaacaac caggttggtt
     6361 tcaccacctc taatccgctg gatgcccgtt ctacgccgta ctgtactgat atcggtaaga
     6421 tggttcaggc cccgattttc cacgttaacg cggacgatcc ggaagccgtt gcctttgtga
     6481 cccgtctggc gctcgatttc cgtaacacct ttaaacgtga tgtcttcatc gacctggtgt
     6541 gctaccgccg tcacggccac aacgaagccg acgagccgag cgcaacccag ccgctgatgt
     6601 atcagaaaat caaaaaacat ccgacaccgc gcaaaatcta cgctgacaag ctggagcagg
     6661 aaaaagtggc gacgctggaa gatgccaccg agatggttaa cctgtaccgc gatgcgctgg
     6721 atgctggcga ttgcgtagtg gcagagtggc gtccgatgaa catgcactct ttcacctggt
     6781 cgccgtacct caaccacgaa tgggacgaag agtacccgaa caaagttgag atgaagcgcc
     6841 tgcaggagct ggcgaaacgc atcagcacgg tgccggaagc agttgaaatg cagtctcgcg
     6901 ttgccaagat ttatggcgat cgccaggcga tggctgccgg tgagaaactg ttcgactggg
     6961 gcggtgcgga aaacctcgct tacgccacgc tggttgacga aggcattccg gttcgcctgt
     7021 cgggtgaaga ctccggtcgc ggtaccttct tccaccgcca cgcggtgatc cacaaccagt
     7081 ctaacggttc cacttacacg ccgctgcaac acatccataa cgggcagggc gcgttccgcg
     7141 tctgggactc cgtactgtct gaagaagcag tgctggcgtt tgaatacggt tatgccaccg
     7201 cagaaccacg cactctgacc atctgggaag cgcagttcgg tgacttcgct aacggtgctc
     7261 aggtggttat cgaccagttc atctcctctg gcgaacagaa atggggccgg atgtgtggtc
     7321 tggtgatgtt gctgccgcac ggttatgaag ggcaggggcc ggagcactcc tccgcgcgtc
     7381 tggaacgtta tctgcaactt tgtgctgagc aaaacatgca ggtgtgcgta ccgtctaccc
     7441 cggcacaggt ttaccacatg ctgcgtcgtc aggcgctgcg cgggatgcgt cgtccgctgg
     7501 tcgtgatgtc gccgaaatcc ctgctgcgtc atccgctggc ggtttccagc ctcgaagaac
     7561 tggcgaacgg caccttcctg ccagccatcg gtgaaatcga cgagcttgat ccgaagggcg
     7621 tgaagcgcgt agtgatgtgt tctggtaagg tttattacga cctgctggaa cagcgtcgta
     7681 agaacaatca acacgatgtc gccattgtgc gtatcgagca actctacccg ttcccgcata
     7741 aagcgatgca ggaagtgttg cagcagtttg ctcacgtcaa ggattttgtc tggtgccagg
     7801 aagagccgct caaccagggc gcatggtact gcagccagca tcatttccgt gaagtgattc
     7861 cgtttggggc ttctctgcgt tatgcaggcc gcccggcctc cgcctctccg gcggtagggt
     7921 atatgtccgt tcaccagaaa cagcaacaag atctggttaa tgacgcgctg aacgtcgaat
     7981 aaataaagga tacacaatga gtagcgtaga tattctggtc cctgacctgc ctgaatccgt
     8041 agccgatgcc accgtcgcaa cctggcataa aaaacccggc gacgcagtcg tacgtgatga
     8101 agtgctggta gaaatcgaaa ctgacaaagt ggtactggaa gtaccggcat cagcagacgg
     8161 cattctggat gcggttctgg aagatgaagg tacaaccgta acgtctcgtc agatccttgg
     8221 tcgcctgcgt gaaggcaaca gcgccggtaa agaaaccagc gccaaatctg aagagaaagc
     8281 gtccactccg gcgcaacgcc agcaggcgtc tctggaagag caaaacaacg atgcgttaag
     8341 cccggcgatc cgtcgcttgc tggctgaaca taacctcgac gccagcgcta ttaaaggcac
     8401 cggcgtgggt ggtcgtctga cccgtgaaga tgtggaaaaa catctggcga aatccccggc
     8461 gaaagagtct gcaccggcag cggctgctcc ggcggcgcaa ccggctctgg ctgcacgtag
     8521 tgaaaaacgt gtcccgatga ctcgcctgcg taagcgtgtg gcagagcgtc tgctggaagc
     8581 gaaaaactcc accgccatgc tgaccacgtt caacgaagtc aacatgaagc cgattatgga
     8641 tctgcgtaag cagtacggtg aagcgtttga aaaacgccac ggcatccgtc tgggctttat
     8701 gtccttctac gtgaaagcgg tggttgaagc cctgaaacgt tacccggaag tgaatgcttc
     8761 tatcgacggc gatgacgtgg tttaccacaa ctatttcgac gtcagcatgg cggtttctac
     8821 gccgcgcggc ctagtgacgc cggttctgcg tgatgtcgat accctcggca tggcagacat
     8881 cgagaagaaa atcaaagagc tggcagtcaa aggccgtgat ggcaagctga ccgttgaaga
     8941 tctgaccggt ggtaacttca ccatcaccaa cggtggtgtg ttcggttccc tgatgtctac
     9001 gccgatcatc aacccgccgc agagcgcaat tctgggtatg cacgctatca aagatcgtcc
     9061 gatggcggtg aatggtcagg ttgagatcct gccgatgatg tacctggcgc tgtcctacga
     9121 tcaccgtctg atcgatggtc gcgaatccgt gggcttcctg gtaacgatca aagagttgct
     9181 ggaagatccg acgcgtctgc tgctggacgt gtagtagttt aagtttcacc tgcactgtag
     9241 acaggataag gcattatcgc cttctccggc aattgaagcc tgatgcgacg cttaacgcgt
     9301 cttatcaggc ctacgggaag caccaatgta ggtcggataa ggcgcttgcg ccgcatccga
     9361 caagcgatgc ctgatgcgac acttacgcgt cttatcaggc ctacaggtga ccgacaatgc
     9421 ccggaggcga tacgaaatat tcggtctacg gtttaaaaga taacgattgc tgaaggatgg
     9481 acagaacaca tgaacttaca tgaatatcag gcaaaacaac tttttgcccg ctatggctta
     9541 ccagcaccgg tgggttatgc ctgtactact ccgcgcgaag cagaagaagc cgcttcaaaa
     9601 atcggtgccg gtccgtgggt agtgaaatgt caggttcacg ctggtggccg cggtaaagcg
     9661 ggcggtgtga aagttgtaaa cagcaaagaa gacatccgtg cttttgcaga aaattggctc
     9721 ggcaagcgtc tggtaacgta tcaaacagat gccaatggcc aaccggttaa ccagattctg
     9781 gttgaagcag cgaccgatat cgctaaagag ctgtatctcg gtgccgttgt agaccgtagt
     9841 tcccgtcgtg tggtctttat ggcctccact gaaggcggcg tggaaatcga aaaagtggcg
     9901 gaagaaactc cgcagctgat ccataaagtt gcgcttgatc cgctgacagg cccgatgccg
     9961 tatcagggac gcgagctggc gttcaaactg ggtctggaag gtaaactggt tcagcagttc
    10021 accaaaatct tcatgggtct ggcgaccatt ttcctggagc gcgacctggc gttgatcgaa
    10081 atcaacccgc tggtcatcac caaacagggt gatctgattt gcctcgacgg caaactgggc
    10141 gctgatggca acgcactgtt ccgccagctt gatctgcgcg aaatgcgtga ccagtcgcag
    10201 gaagatccgc gtgaagcaca ggctgcacag tgggaactga actacgttgc gctggacggt
    10261 aacatcggtt gtatggttaa cggcgcaggt ctggcgatgg gtacgatgga catcgttaaa
    10321 ctgcacggcg gcgaaccggc taacttcctt gacgttggcg gcggtgcaac caaagaacgt
    10381 gtaaccgaag cgttcaaaat catcctctct gacgacaaag tgaaagtcgt tctggttaac
    10441 atcttcggcg gtatcgttcg ttgcgacctg atcgctgacg gtatcatcgg cgcggtagca
    10501 gaagtgggtg ttaacgtacc ggtcgtggta cgtctggaag gtaacaacgc cgaactcggc
    10561 gcgaagaaac tggctgacag cggcctgaat attattgcag caaaaggtct gacggatgca
    10621 gctcagcagg ttgttgccgc agtggagggg aaataatgtc cattttaatt gataaaaaca
    10681 ccaaggttat ctgccagggc tttactggta gccaggggac tttccactca gaacaggcca
    10741 ttgcatacgg cactaaaatg gttggcggcg taaccccagg taaaggcggt actacccacc
    10801 tcggcctgcc ggtgttcaac accgtgcgtg aagctgttgc tgctactggc gctaccgctt
    10861 ctgttatcta cgtaccagca ccgttctgca aagactccat tctggaagct attgatgcag
    10921 gcatcaaact gattatcacc atcactgaag gcatcccgac gctggatatg ctgactgtga
    10981 aagtgaaact ggacgaagcc ggcgttcgta tgatcggccc gaactgcccg ggcgttatca
    11041 ctccgggtga atgcaaaatc ggtatccagc cgggtcacat tcacaaaccg ggtaaagtgg
    11101 gtatcgtttc ccgttccggt acactgacct atgaagcggt taaacagacc acggattacg
    11161 gtttcggtca gtcgacctgt gttggtatcg gcggtgaccc gatcccaggc tctaacttta
    11221 tcgacatcct cgaaatgttc gaaaaagatc cgcagaccga agcgatcgtg atgatcggtg
    11281 agatcggcgg tagcgctgaa gaagaagcgg cagcgtacat caaagagcac gttaccaagc
    11341 cagttgtagg ttacatcgct ggtgtgactg cgccgaaagg caaacgtatg ggccacgcgg
    11401 gtgcgatcat tgccggtggg aaagggactg cggatgagaa attcgctgct ctggaagccg
    11461 caggcgtgaa aaccgttcgc agcctggcgg atatcggtga agcactgaaa actgttctga
    11521 aataaatatc tgtaataaga aatagccctc gccgcttccc tctacaggaa tggcgaaggg
    11581 ctgtcggttt cgacatggtt ggccatcgta tgatggcctt ttttgtgctt atcgcgatga
    11641 ttttcgctgc gctatcaggg taaatttata gtcatcggta ttaaaagcgt tgcggctata
    11701 ttcaaacacc cgaccatcaa ctaaatatcc acgcgatact ttttcaagaa tcggctttgt
    11761 ctggctgata ttaagcagac ggctcatctc ttcggttggc atcagaggaa tgatttcctg
    11821 ttcgctacga tcgataacca tcttcttcac ttcttcaata aagtgatatt tcgaattttc
    11881 catgacctgc caggtgagat ccgggaacaa cgcaagcggc atccaggttt cttccagcgc
    11941 cattggcttt tgcttgcgat agcgcacgcg cttcacatgc cacacacgat cctgcgcggt
    12001 gatttgtagc tgttgctgaa gaaaatcgtc agccggaatc acttcaaata tcaggacttc
    12061 actgtgtgta tcgacgtgac ggtccgacag tttttcatca aaactggtta actgaaaaat
    12121 atcgtaattg acccgctctt ctttgacgta agtcccgctg ccctgaatgc tttcgaggat
    12181 ctgctgctcg actagctggc gcaaagcctg acgcaccgta acccggctga cgccaaactc
    12241 tgtttgtagc gctgattcag tgggtaacgc atcgccaggt ttaagctcgc cacgcgcaat
    12301 ttgttcacga atgcgatcgg caatctgccg gtataagggc ttgtgtccca tttttagtat
    12361 ctcattaata cgaatttagc cattatgccc gataaattca tcctgtaaat aatgcaaata
    12421 caatacaaat aatttcaatc aagtgaaatt gatcatataa tggtattgtt ttatcgggca
    12481 cactggcgcg actataaaaa cgatcaagtg aggatcatga tgaatctgac gactctgacc
    12541 caccgcgatg cgttgcgtct gaatgcgcgc tttaccagcc gtgaagaggc catccacgcg
    12601 ttgactcaac gtcttgctgc tctggggaaa atttccagta ctgagcaatt tctggaagaa
    12661 gtgtatcgcc gtgaaagcct tggcccgacc gccttaggtg aagggttggc tgtgccgcat
    12721 ggcaaaactg ctgcggtaaa agaagcggcg tttgcggtcg ccacactcag cgagccgctt
    12781 cagtgggaag gcgttgatgg cccggaagca gttgatttag tggtgctgct ggcgattccc
    12841 cccaatgaag cgggtactat gcatatgcaa ctgctgacag cgctgaccac gcgccttgcg
    12901 gatgatgaga ttcgggcgcg tatacagtcg gcgacgacgc ctgatgagtt gctctcggcg
    12961 ctggatgaca agggaggcac gcaaccttct gcctcttttt ccaacgcgcc aactatcgtc
    13021 tgcgtaccgg cctgtccggc gggtattgct cacacctata tggctgcgga atatctggaa
    13081 aaagccggac gcaaactcgg cgtaaatgtt tacgttgaaa aacaaggcgc taacggcatt
    13141 gaagggcgtt taacggcgga tcaactcaat agtgcaaccg cctgtatttt tgcggctgaa
    13201 gtcgccatca aggagagtga gcgttttaat ggcattcccg cgctttcagt gcctgttgcc
    13261 gagccgattc gccatgcaga agcgttgatc caacaagcgc ttaccctcaa gcgtagcgat
    13321 gagacgcgta ccgtacagca agatacgcaa ccggtgaaaa gtgtcaaaac ggagctgaaa
    13381 caggcactgt tgagcggaat ctcttttgcc gtaccgttga ttgtcgcggg gggcacggtg
    13441 ctggcggtcg cggtattact gtcgcaaatc ttcgggctac aagatctgtt taatgaagaa
    13501 aactcctggc tgtggatgta ccgcaagctg ggcggcgggc tgctcggaat tttgatggta
    13561 ccggtgctcg cggcctatac tgcctattct ctggcagata aaccggcgtt agcgccaggc
    13621 tttgcggctg gacttgccgc caacatgatc ggctccgggt ttctcggcgc ggtcgttggc
    13681 ggattgatag ccggttactt gatgcgctgg gtgaaaaatc acttgcgtct tagcagtaaa
    13741 ttcaatggat tcctgacttt ttatctctac ccggtgctcg gtacgttggg agcgggcagt
    13801 ctgatgctgt ttgtggtggg agaacctgtc gcatggatca ataactcgct taccgcctgg
    13861 ctgaacggtc tgtcaggaag taacgcgctg ttgctgggtg ccattttcgg ttttatgtgt
    13921 tcctttgatc ttggagggcc agtgaacaaa gccgcttatg cattctgcct gggcgcaatg
    13981 gtgaacggcg tttacggccc gtatgccatt ttcgcctccg tcaaaatggt ttcggcattt
    14041 accgtaaccg cttccacgat gctcgcaccg cgcctgttta aagagtttga aattgagacc
    14101 gggaaatcca cctggctgtt agggctggca ggtattaccg aaggggcgat cccgatggcg
    14161 attgaagatc cgctgcgggt tattggttcg tttgtgctgg gctctatggt aacgggcgct
    14221 attgtcggtg cgatgaatat cggcctttcg acacccggtg ccggcatttt ctcgctcttt
    14281 ttacttcatg ataatggcgc gggcggtgtt atggcggcaa ttggctggtt tggtgcggca
    14341 ttggtggggg ctgcaatctc gactgcaatt ctcctgatct ggcggcgtca cgcggttaag
    14401 catggcaact atctgactga tggcgtaatg ccataaacaa aaaggaaacg acgatgaaag
    14461 cggtatctcg tgttcacatc accccgcata tgcactggga tcgagagtgg tatttcacca
    14521 ccgaagagtc acgtattctg ctggtcaata atatggaaga gatcctgtgc cgactggaac
    14581 aggacaacga atacaaatat tacgtactcg acgggcaaac ggcgatcctc gaagattatt
    14641 tcgcggtgaa accggaaaac aaagaccgtg tgaagaaaca ggtagaagcc ggcaagttga
    14701 ttatcggccc ctggtatacc cagaccgata ccacgattgt ttctgcggaa tccatcgtcc
    14761 gtaatctgat gtacggaatg cgtgactgcc tcgcgtttgg cgagccgatg aaaataggtt
    14821 atttaccaga ttcctttggc atgtccgggc aactgccgca tatctacaat ggatttggca
    14881 ttacccgcac catgttctgg cgcggatgtt cggagcgcca cggtactgat aaaaccgagt
    14941 ttttgtggca aagcagtgac ggtagcgaag tgacggcgca ggtgctgccg ctgggctacg
    15001 ccataggtaa gtacttacct accgacgaaa acggattacg taaacgcctc gacagttatt
    15061 ttgacgtgct ggaaaaagcg tctgtaacca aagagatttt gctgccgaat ggtcatgacc
    15121 agatgccatt gcagcaaaat atcttcgaag tgatggataa gctacgtgag atctaccctc
    15181 aacgtaagtt tgtgatgagc cgctttgaag aggtatttga gaagatcgaa gcgcagcgtg
    15241 aaagtctggc caccctgaaa ggtgaattta tcgatggcaa atatatgcgc gtacatcgca
    15301 ccatcggttc cacgcgcatg gatatcaaaa ttgcccacgc acgtattgaa aataagattg
    15361 ttaatctgct ggaaccgctg gcaacgctgg cctggacgtt gggttttgaa taccaccacg
    15421 gcttgctgga gaaaatgtgg aaagagatct taaaaaatca tgcccacgac agtatcggtt
    15481 gttgttgcag tgacaaagtt catcgcgaaa ttgtcgcccg cttcgaactg gctgaagaca
    15541 tggcggataa tctgctgcgt ttctacatgc gcaaaattgc cgataacatg ccacagagtg
    15601 atgccgacaa actcgtgctg tttaacctga tgccctggcc gcgggaagaa gttattaaca
    15661 ccactgtgcg gctgcgcgcc agccagttta atctacgaga cgatcgcggt cagcctgtac
    15721 cgtattttat tcgccatgcc cgtgagatcg atccaggcct aatcgatcgg caaatagttc
    15781 attacggtaa ttacgatccc tttatggagt ttgatataca gatcaaccag attgtccctt
    15841 ctatgggcta tcgcacgctt tatatcgaag cgaatcagcc tggcaacgtg attgcggcaa
    15901 aaagtgacgc tgaagggata ctggaaaatg ctttctggca aattgcgctc aatgaggatg
    15961 gttctctgca actggtagat aaagacagcg gtgtgcgcta tgaccgagta ttgca
//
LOCUS       DAJTYD010000080        15635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTYD010000080 DAJTYD010000000
VERSION     DAJTYD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.080"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            338..430
                     /locus_tag="OO585_002310"
                     /pseudo
     CDS             338..430
                     /locus_tag="OO585_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            434..613
                     /locus_tag="OO585_002311"
                     /pseudo
     CDS             434..613
                     /locus_tag="OO585_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            947..3226
                     /gene="ydeP"
                     /locus_tag="OO585_002312"
     CDS             947..3226
                     /gene="ydeP"
                     /locus_tag="OO585_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6629879.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3474..3671
                     /gene="safA"
                     /locus_tag="OO585_002313"
     CDS             3474..3671
                     /gene="safA"
                     /locus_tag="OO585_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6629880.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3746..4507
                     /gene="ydeO"
                     /locus_tag="OO585_002314"
     CDS             3746..4507
                     /gene="ydeO"
                     /locus_tag="OO585_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6629881.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4909..6591
                     /locus_tag="OO585_002315"
     CDS             4909..6591
                     /locus_tag="OO585_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6629882.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6643..7800
                     /locus_tag="OO585_002316"
     CDS             6643..7800
                     /locus_tag="OO585_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6629883.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7766..7888)
                     /locus_tag="OO585_002317"
     CDS             complement(7766..7888)
                     /locus_tag="OO585_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629884.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8097..9782
                     /locus_tag="OO585_002318"
     CDS             8097..9782
                     /locus_tag="OO585_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6629885.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9820..12192
                     /locus_tag="OO585_002319"
     CDS             9820..12192
                     /locus_tag="OO585_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6629886.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12249..15032
                     /locus_tag="OO585_002320"
     CDS             12249..15032
                     /locus_tag="OO585_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6629887.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15394..>15635
                     /locus_tag="OO585_002321"
     CDS             15394..>15635
                     /locus_tag="OO585_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6629888.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4552 a         3399 c         3427 g         4257 t
ORIGIN      
        1 cgtggtgacg gcagttgaaa ccaatgtgtt gaggttgcat ggacggttaa gccgagaata
       61 cggtagtaag tgagatatgg ggtttggtcg ttgttaatga gcaaccatga ccatgaccgc
      121 caacgactgg attctccctt ggttatgttg gtgcgcataa tgtatattat gttaaatcat
      181 atctgagtga tgcgaagtgc ttttctggta gtcgttattc gttcagggtt ccagaactta
      241 agcctctcct ctttcataat tggcctgtgc atgacggata taggctggct ggtatgcaga
      301 tttgccgttt gccgcatctt cccagcgttg aagactcaag ctggcaaacc gcgtgcaaat
      361 gagcacgccc tctttccatc aacacttcag gcagcttgcg ggaatgagtc cactacggta
      421 tcagaaatgg gaagtgggtg tctctctgat gcgtaatgga aaaatactgg ctacgggaga
      481 aaatgtctcc ctggggacag taaataaaag caaagtatct ctgggtttaa gcgcaaccta
      541 tggacaaaca ggaaacaagg ttgcagccgg aaccgtccag tcagtcattg gtgtcacttt
      601 tatctatgaa taattgtgat tttgatctta gtctatgcgc tccattcacg aaaattgcgc
      661 ggataattat tttgtgaagg ctattatcct acacctgtaa gaaaatccgc gcacaattga
      721 agccagagtc ttatttttta tattgaaaaa tatgttaatt taaacactat gagagatgat
      781 gcttaactta tcgtatttaa tctattgttt aacgataata gattaaaaat agaaagagag
      841 tagtccccta ctcttatata tccatgttgg cgataatccc tttgtatgta ctgtgcatca
      901 tcgctattac aaatcctaat aattcatttc cacacaggat aagtagatga agaaaaaaat
      961 tgaatcctac cagggtgctg caggtggttg gggtgctgtt aaatccgtag cgaatgcagt
     1021 acgtaagcag atggatatac gccaggatgt tattgccatg tttgacatga ataagccaga
     1081 gggctttgac tgtccgggtt gtgcatggcc agatcctaag cacactgcgt cattcgacat
     1141 ttgtgaaaac ggcgcaaaag caatcgcctg ggaagtcacg gataagcagg taaatgcctc
     1201 tttctttgct gagaatacgg ttcaatcatt acttacctgg ggagatcacg agcttgaggc
     1261 tgcgggacgt ctcactcagc ctttgaaata tgatgccgtc agcgactgtt acaagccatt
     1321 aagctggcaa caagctttcg acgaaattgg cgcacgcctt caaagctata gtgatcccaa
     1381 tcaggttgaa ttctatactt cgggccgcac ttccaatgaa gctgcctttc tttatcagct
     1441 ttttgcccgt gaatacggga gcaataactt tcccgactgc tccaacatgt gccatgaacc
     1501 gaccagtgtg ggtttggcag cgagtatcgg cgtgggtaaa gggactgtgt tgctggaaga
     1561 ctttgagaag tgcgatttag tcatttgcat tgggcataac cctggtacaa accacccccg
     1621 catgctgact tcgttgcgcg ctttagtgaa acggggagcg aaaatgatcg ccatcaatcc
     1681 tttacaggaa cgtggcctgg agcgatttac cgcaccgcaa aacccgtttg aaatgctgac
     1741 gaactctgag actcagttgg ccagtgcgta ctacaacgtg cgcattggtg gcgatatggc
     1801 gttgctcaag gggatgatgc gcctgttaat tgagcgcgat gatgctgcaa gtgccgcagg
     1861 tcggccctca ttgcttgatg acgaatttat tcaaacgcat accgtcggct ttgacgagct
     1921 acgccgtgac gttctcaatt ccgagtggaa agatatcgaa catatttctg gactaagtca
     1981 gacacaaatc gccgaactgg ctgacgcata tgccgctgcc gatcgcacca ttatctgtta
     2041 cggaatgggg atcactcagc acgaacatgg tacccagaat gtacagcaac tggtcaatct
     2101 gctgttgatg aaaggtaaca ttggcaagcc tggtgcgggt atctgcccac tacgaggtca
     2161 ctctaatgta cagggcgacc gaaccgtcgg tatcaccgag aaaccgtctg cagagtttct
     2221 ggctcgcctg ggtgagcgct atggcttcac cccacctcat gcacctggac atgctgcaat
     2281 tgccagcatg caagcaatat gcacggggca ggctcgagca ttgatctgca tggggggcaa
     2341 ctttgcactg gcaatgccag atcgggaagc gagcgctgta ccgttaacgc aattagattt
     2401 ggcggtacac gtagccacta agcttaaccg ctctcatctg ctgaccgcac ggcatagcta
     2461 tattctgccg gttctggggc gtagcgagat tgacatgcaa aaaagcggtg cgcaggcggt
     2521 aaccgttgag gattcaatgt cgatgattca tgcctcgcgt ggcgtgttaa aacccgccgg
     2581 tgtaatgctg aaatcagagt gtgcagtggt cgcgggaatc gcgcaggcag cactacccca
     2641 gagcgtggta gcctgggagt atctggtgga agattatgat cgcattcgca atgacattga
     2701 agctgtgtta ccagagttcg ccgactataa ccagcgcatc cgacatcctg gaggttttca
     2761 cctgataaat gcagctgctg aaaggcgctg gatgacgccg tcaggtaagg ctaatttcat
     2821 taccagcaaa gggctgttag aagatccctc ttcagcgttt aacagtaagc tggtcatggc
     2881 gacagtacgc agccacgatc agtacaacac gacgatttat ggtatggatg atcgctatcg
     2941 aggggtattc ggtcaacgag atgtggtctt tatgagtgct aaacaagcta aaatttgccg
     3001 tgtaaaaaac ggcgaaagag ttaatcttat tgcgcttacg ccagacggta agcgcagctc
     3061 acgccgcatg gatagattaa aagtggtcat ttaccctatg gctgaccgct cactggtgac
     3121 ctattttcca gaatcgaatc acatgctaac tcttgataac cacgatccat taagtggcat
     3181 tcctggttat aaaagtattc cggttgaatt agaaccatca aattaattcg cctcctcatt
     3241 tcttctgctg tcatccgcac agcagaagaa tttctcattg actattatcg cgcaatttgc
     3301 tcacattgat caaattaaac cacacactat aagatataaa cctctgccta cagctgtaag
     3361 aaactccgct cagtactgaa gtgccagtcc tatttcctct tttctccagc ctgttatatt
     3421 aagcatactg attaacaatt tttaacgtta tccgctaaat aaacatattt gaaatgcatg
     3481 cgaccacagt gaaaaacaaa atcacgcaaa gagacaacta taaagaaatc atgtctgtaa
     3541 ttgtggttgt cttattactg acccttacgt tgatagccat tttttcggca attgatcagc
     3601 tgggtatttc agaaatgggt cgcattgcaa gagatcttac acatttcatt atcaatagtt
     3661 tgcaagactg aaaataaaca gcaaattata aatatgaaat gttaaaaaag tatcgataaa
     3721 aactttattg ttttaaggag ataaaatgtc gctcgtttgt tctgttatat ttattcatca
     3781 tgccttcaac gctaacattt tagataaaga ttacgccttc tctgacggcg agatcctgat
     3841 ggtagataac gctgttcgta cgcattttga accttatgag cggcatttta aagagatcgg
     3901 atttactgaa aataccatta aaaaatatct acaatgcact aacatccaga cagtgacggt
     3961 gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg actggattgc ttaatgaaat
     4021 gattgcttat ctcaactcgg aagaacgcaa tcatcataat ttttcagaac ttttgctttt
     4081 ttcttgcctg tctatttttg ccgcatgcaa aggtttcatt acactattaa ctaacggtgt
     4141 gctatccgtt tccggaaaag tgagaaatat tgtcaacatg aagctggcgc acccatggaa
     4201 gctgaaagat atttgtgact gcctgtacat cagtgaaagc ctgttgaaga aaaaactaaa
     4261 gcaagagcaa acgacattct cacagattct attagatgcc agaatgcagc acgcaaaaaa
     4321 tttgatacgc gtagaaggtt cagtcaataa aattgccgaa caatgtggtt atgccagtac
     4381 ttcttatttt atttatgcgt tccgcaaaca tttcggcaac agtccgaaga gagtttctaa
     4441 ggagtaccgt tgtcaaagtc acacgggtat gaatacgggc aacacgatga gtgctttagc
     4501 tatttgatta tttgctaacg agtagtcaac cacgcacgct acgtaagaat tatatggggc
     4561 agccattccc tgccccgcgt tgtttttagg cgatatattt attgaaataa ataagtgaca
     4621 tccatcacat atttatgcac ttgcataacc tgttgcatga ttatttatgt tctcaattca
     4681 gcatcttgtc agtaaaatgc aataatttat taaatatcaa taaattagtt gtttatcggc
     4741 gagaaattac ttaatagaac agaaagtaat gtcaacgctt tatggactgt tatttccctt
     4801 tttttagcta aatctgctat ctctttatgt gactaacttc acttacatcc acttatttct
     4861 cttcgtaaaa ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca
     4921 ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca
     4981 tttgctgctc atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc
     5041 tcagacttta cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc
     5101 atggatgatc ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca
     5161 atggaaaatc gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct
     5221 gcacaaaaat caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac
     5281 ggctatgtgg cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct
     5341 cccgcccgct ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa
     5401 actttcttgc ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg
     5461 cacttgtcaa aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat
     5521 gacaacttca ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac
     5581 tttatgggat tccacgctgc aggaacggca tattacaact ccccttcatt gttcaaaaat
     5641 cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc
     5701 gttgttgatc gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct
     5761 ccgcacctgc caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt
     5821 agtcaaacag cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc
     5881 attctcgaac aactgaagaa aaacggacag tatgataata ctattattct ctttacctcc
     5941 gataatggtg cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag
     6001 agtcagacct atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt
     6061 caacccggta attatgacaa gctgatttcc gcaatggatt tctacccgac agctcttgat
     6121 gcagccgata tcagcattcc aaaagacctt aagctggatg gcgtttcctt gctgccctgg
     6181 ttgcaagata agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct
     6241 cactggtttg acgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat
     6301 cagtcagacg attacccgca taaccccaac actgaggact taagccaatt ctcttatacg
     6361 gtgagaaata acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac
     6421 aatctgacgg atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa
     6481 gagatgcaag gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta
     6541 aatcaggaga agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt
     6601 catgcggcgg aattttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag
     6661 ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg
     6721 cagtttactc atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat
     6781 atcgcagcgt ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct
     6841 ggcctggatt ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt
     6901 atttttaatg cattacaaac gaatggcatt ttattgaata atgaatggtg ttcctttctc
     6961 aaagaacatg aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt
     7021 tacagacgca gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt
     7081 ctgaaatcat atcaagttga gtttaatacg ttaaccgtca ttaataacgt taatgtccat
     7141 taccctcttg aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc
     7201 gaattgctcg aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc
     7261 cgtatcattg atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt
     7321 atgcaatggg ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc
     7381 agccgttttt tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat
     7441 ctggttgttg aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac
     7501 aaaattggaa acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg
     7561 caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc
     7621 ggttgtccta agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa
     7681 ggttataaaa tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct
     7741 aagaacagag taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa
     7801 taacgctctc ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga
     7861 cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac
     7921 gtggtatgcg tcagggcatt agcaataccc ttgattatca atagcataac cataaccata
     7981 accactgaaa attaaaaatt tctatatatt tcatattgtt aaatgatact taaataaatt
     8041 tcatcagtaa atcattgcca tagataatca ttggatggca caatcaattt tcgctaatga
     8101 taaccattcc cattacgttt tgtatgttga tagcaaagta tctctgtctg ctgaagccct
     8161 tttggctgcg taagaataat aaaacttcag tgttgttaat cattattatc ctcgcaatga
     8221 tccttggcgt ggttaaaatt caggtctggt taaacgactg gaataatgat tttttcaatg
     8281 cgctaagcca gaaagaaacc gataagcttt ggcagcttgt tctttggttc cctgccctgc
     8341 tggggatctt tgtattaata tccgtaaaca aaacatggtt aattaaacta ctgactattc
     8401 gctggcgcga atggttaaca gattactacc ttaatcgctg gtttgcagat aaaaactact
     8461 acttcacgca gatctacggc gagcataaaa atacagataa ccctgaccaa cgtatcgctg
     8521 aagacattct cttactgatc agcaaaacgc tcagtctttc atttggcttc atccagtcac
     8581 ttagcatgct gatcaccttt accgttattc tttggcaaag cgcgggtacg ctctcattca
     8641 ctgttggtgg aacagaatgg aatatccagg ggtatatggt ctataccgtc gtgctcattg
     8701 tgatcggtgg aactttattt acccataagg ttggtaagcg tattcgtccg cttaatgtgg
     8761 aaaaacaacg tagcgaagcg acatttcgaa ctaatcttgt gcagcataat aagcaggcag
     8821 aactgattgc gctaagcaat gctgagtctc tacaacgcca ggagttgcgc gacaattttc
     8881 ataccattaa agagaactgg catcgtttaa tgaatcgtca gcggtggctt gattactggc
     8941 aaaatattta ttcgcgctcg cttagcgttc ttccctactt tctgttattg ccgcagttta
     9001 ttagcgggca gatcaatctg ggcggactga tgaaatcgcg ccaggcattt atgctagtat
     9061 cgaacaattt aagctggttt atttataaat atgacgaact tgctgaactg gctgcggtta
     9121 tcgatcgctt gtatgagttc catcaactca ctgaacagcg ccctacgaat aagcctaaaa
     9181 attgccaaca tgcggtacaa gtggctaatg cgagtattcg tacgcctgat aataagatca
     9241 tattagagaa cttgaacttt catgtttcgc caggcaaatg gctattactg aaaggctact
     9301 ctggtgcggg aaaaaccaca ctgcttaaaa cattatccca ctgctggccg tggtttaaag
     9361 gtgatatttt ttctcctgct gacagttggt atgtgtcaca aacaccgtta atcaaaaccg
     9421 gcttactgaa agagattatt tgtaaagcac ttcccctgcc cgtagacgat aaatcgttga
     9481 gcgaagtact gcatcaggtt ggtcttggga aattggctgc gcgtattcat gaccacgatc
     9541 gctggggaga tattctttcc agcggcgaaa aacaacgtat cgccctggca cgattaattt
     9601 tacgacgtcc gaaatggata tttcttgacg aaactacctc tcatcttgag gaacaagagg
     9661 ctatccgctt actgcgttta gtgcgtgaaa aactacccac aagcggcgtc attatggtta
     9721 cacatcaacc cggtgtctgg aacctggccg atgatatttg tgacattagc gcggttttat
     9781 aaaaacaata aaagccctgc tgtacggaga tataaataaa tgaagcgagt tcttattcct
     9841 ggcgtcattt tatgtggcgc tgatgtggcg caggccgtcg atgacaaaaa catgtacatg
     9901 catttttttg aagagatgac ggtctatgct cctgtccctg tacccgtaaa cggcaacacg
     9961 cattacacca gtgaaagcat cgagcgttta ccgaccggga atggcaatat cagcgatctg
    10021 ctgagaacca accctgcggt acgcatggat tcaacgcaaa gtacctcgtt gaaccaggga
    10081 gatattcgcc ctgagaaaat ctctattcac ggtgcgtcgc cctaccagaa tgcctatttg
    10141 attgacggta ttagtgcaac taataacctg aacccagcga atgagtccga tgccagtagt
    10201 gcaaccaata ttagcgggat gtcacagggg tattatcttg atgtcagctt actggacaat
    10261 gtgacgcttt atgacagttt tgtgccggtt gaatttggtc gcttcaatgg cggggtaatt
    10321 gatgcaaaga tcaaacgctt caacgctgat gatagcaagg tgaaattggg ttatcgcact
    10381 acgcgttcgg actggttaac atcgcatatc gatgagaata acaagagcgc atttaatcaa
    10441 ggttcttcag gaagtactta tttctctcca gattttaaaa agaactttta taccttgtcg
    10501 tttaatcagg agctggctga taactttggc gttaccgccg gtttatcgcg ccgccagtct
    10561 gatatcaccc gcgcggatta tgtttcgaat gacggcattg tcgtcggtcg ggcacagtat
    10621 aaaaacgtta tcgatactgc attgagcaaa tttacctggt ttgccagcga ccgctttacc
    10681 cacgatttaa ccttaaaata taccggctcc agccgtgatt ataataccag caccttcccg
    10741 cagtctgatc gcgaaatggg taataaatcc tatggtcttg catgggatat ggatacgcaa
    10801 ctcgcatggg ccaaactacg taccaccgtt ggttgggatc atattagtga ttatacccgt
    10861 cacgatcatg acatctggta caccgaactt tcatgtacat atggtgatat tacagggcgt
    10921 tgtacccgtg gcggattagg acacatttcc caggctgtag ataattacac cttcaaaaca
    10981 cgcctggact ggcaaaaatt cgccgtgggt gatgtttcgc atcaacccta ctccggcgcg
    11041 gaatacatct attccgatgc atggactgaa cgccataacc agtctgaatc ctatgtgatt
    11101 aatgctgcag gaaagaaaac taaccatacc atttaccata aaggtaaagg cagcctagga
    11161 attgacaact acacgctgta tatggcggat cacattagct ggcggaatgt gtcgttaatg
    11221 cccggtgtgc gttatgacta tgacaactat ctgtcaaacc acaatatctc cccgcgcttt
    11281 atgacggaat gggatatttt tgctgatcaa acctcaatga ttaccgccgg ttataaccgt
    11341 tactatggcg ggaatattct tgatatggga ttacgtgata tccgcaatag ctggacggaa
    11401 tcggtatcag gtaataaaac cctgacgcgt tatcagaatt tgaaaacgcc ttataacgat
    11461 gaactggcaa tgggattgca gcaaaaaatc ggtaagaacg ttattgcacg cgcaaactat
    11521 gtttaccgtg aagcgcatga tcaaatcagc aaaagcagtc gtaccgacag cgcgactaaa
    11581 accaccatta ctgaatataa caacgatggc aaaaccaaaa cgcattcgtt taacctcagt
    11641 tttgaactgg ccgaacccct gcatatccgc caggtagata ttaacccgca aattgtcttt
    11701 agctatatca agagcaaggg caacttgtcg ttaaacaatg gttatgagga gagcaatacc
    11761 ggtgataacc aggtggttta taacggtaat ctggtctctt acgatagcgt tccagtggca
    11821 gattttaata acccattaaa gatctcctta aacatggatt tcacgcatca accgagcggg
    11881 ttagtgtggg cgaatacgct ggcgtggcaa gaagcgcgta aagctcgcat tatccttggt
    11941 aagacaaatg cgcaatacat cagcgaatat tcagattaca agcagtatgt tgacgaaaaa
    12001 ctggatagca gcctgacctg ggacacccgc ttgtcctgga cgccacaatt tctgaaacaa
    12061 caaaacctga cgatcagtgc cgatattctc aatgtactgg atagcaaaac cgctgttgat
    12121 acaacgaata ccggtgtggc gacctacgcc agtggccgta ctttctggct tgatgtcagc
    12181 atgaaatttt aatagttcgg attttactta accgggtaaa ttcgcccggt tttcgcatgg
    12241 agataattat gagaaacctc tgtttcttac tgacgttagt ggcaactctg ttgctccccg
    12301 ggcggctgat tgccgccgcc ttaccgcagg atgaaaagtt aattaccggg caactggaca
    12361 atggcttgcg atatatgatt tatccgcatg ctcaaccaaa ggatcaggta aatttatggc
    12421 tgcaaattca taccggttca ttgcaggaag aagacaatga gcgcggcgtg gctcattttg
    12481 tagaacatat gatgtttaac ggcacaaaaa catggccggg taataaagtc atcgaaacat
    12541 ttgagtcaat gggcctgcgt tttggtcgcg atgttaatgc ctataccagc tatgacgaaa
    12601 cggtgtatca ggtgagtttg ccgactacgc agaaacaaaa tctgcaacaa gtgatggcaa
    12661 tcttcagtga atggagtaat gccgcaacct ttgaaaaact cgaagtagac gctgaacgtg
    12721 gcgtaattac tgaggaatgg cgtgcccatc aggatgcgaa atggcgcacc tctcaggcgc
    12781 gccgcccttt cctgctggct aatacccgta atttagaccg tgaacctatc ggcctgatgg
    12841 atactgtcgc cacggtcaca ccggcacaat tgcgccaatt ttatcaacgc tggtatcaac
    12901 caaataatat gacctttatc gtggtcggcg atatcgacag taaaaaagcg ctggcgctga
    12961 taaaggataa tttaagtaag cttccggcta acaaagcagc tgaaaatcgc gtctggccga
    13021 caaaagccga aaaccacctg cgctttaata tcattaatga taaagaaaac cgggtgaacg
    13081 gcatcgcact ctattatcgc ctgccaatgg tacaagtgaa cgatgagcaa agctttatcg
    13141 aacaagctga atggagcatg ttagttcagc tgttcaatca acgtctgcag gaacgcatac
    13201 agtcgggcga gttgaagact atttctggcg gcactgcgcg cagcgttaaa attgcacccg
    13261 attatcagtc gctgtttttc cgtgtaaatg cacgagacga taatatgcag gatgctgcga
    13321 atgcattaat ggcagagttg gcaaccattg atcagcatgg tttttctgct gaagaactcg
    13381 atgatgtcaa atctacccgc ctcacctggc tgaaaaatgc ggttgatcag caagctgaac
    13441 gtgatttacg tatgctgacc agtcgcctgg catccagctc attaaataat acgccgttct
    13501 tgtcgccgga agagacatat caactttcga aacgtctgtg gcagcaaatt accgtgcaaa
    13561 gtctggcgga aaaatggcag cagttaagaa agaaccagga cgcattttgg gagcaaatgg
    13621 taaacaatga gcttgccgcc aaaaaagcat tgtctcctgc ggctatcctg gcgctggaaa
    13681 aagagtacgc caacaaaaag ctggcggctt acatcttccc aggcagaaat ttatcgttaa
    13741 cagtagacgc tgacccacag gcggaaatta gcagcaaaga aacgctggct gagaatctga
    13801 catcattaac actttccaat ggtgccaggg ttattctggc aaaatccgcg ggtgaagagc
    13861 aaaagctaca aattactgcc gtatcgaata aaggcgattt aagtttccct gcgcagcaaa
    13921 aatcacttat cgcgctggca aataaagcag taagcggaag cggcgttggt gagctctcct
    13981 cttccagcct gaaacgctgg agtgcggaaa attcggtaac catgagcagt aaagtcagtg
    14041 gcatgaatac gttgctctcc gttagcgcgc ggactaataa ccctgaacct ggttttcagt
    14101 tgattaacca gcgaatcacc cacagcacga ttaacgacaa tatttgggca tcgctacaaa
    14161 atgctcaaat tcaggcgttg aaaacgctcg accagcgtcc agcggagaaa ttcgcccagc
    14221 agatgtatga gacgcgctat gctgatgacc gcacgaaatt actgcaagaa aatcagattg
    14281 tacagtttac tgccgcagat gcgctggctg ccgatcgcca gttgttttca tctccagcgg
    14341 atatcacgtt tgtcattgtc ggtaatgtct cagaagacaa actcgtggcg ttaattacgc
    14401 gttacttagg atcaatcaaa cactctgatt cgccattagc cgcaggtaaa ccattaactc
    14461 gcgcgacgga caacgcatcg gttactgtaa aagaacaaaa tgaacctgtg gcacaggttt
    14521 cacagtggaa gcgttatgat tcccggacac ctgttaatct ggcgacgcgt atggcgctcg
    14581 atgcttttaa cgtcgcactg gcaaaagatc tacgtgttaa tattcgtgaa caggcatctg
    14641 gagcatacag cgtttcttct cgcctctcgg ttgatcctca ggccaaagat atcagtcatt
    14701 tgctggcttt tacttgtcaa ccagaacgac atgatgaact gttaacgttg gcgaatgaag
    14761 tgatggttaa gcgtctggct aaagggatca gtgagcaaga actgaatgaa taccagcaaa
    14821 acgttcagcg cagcctcgat atccaacagc gtagcgttca acaattagcg aacactattg
    14881 taaatagtct tattcaatat gacgatcctg cagcatggac tgagcaggag caattgttga
    14941 aacaaatgac ggtagagaat gttaacactg ccgttaaaca atatctttct catccggtaa
    15001 atacttatac cggagtatta ttgccaaaat aataacagcc ccgtcaacac atcgttggcg
    15061 gggattttag caatattcgc tatttttatg taataatttt ataaatgcgt tcaaaataat
    15121 aatcaagtac taatagtgat attttaaggt ctgattttta cgtgataatt caggagacac
    15181 agaatgcgca taaaaataac agcataaaac accttaccac cacccaagaa tttcatattg
    15241 tattgttttt caatgaaaaa acattattcg cgtaatatct cacgataaat aacattagga
    15301 ttttgttatt taaacacgag tcctttgcac ttgcttactt tatcgataaa tcctactttt
    15361 ttaatgcgat ccaatcattt taaggagttt aaaatggata agaagcaagt aacggattta
    15421 aggtcggaac tactcgattc acgttttggt gcgaagtcta tttccactat cgcagaatca
    15481 aaacgttttc cgctgcacga aatgcgcgac gatgtcgcat tccagattat caatgacgaa
    15541 ttatatcttg atggcaacgc tcgtcagaac ctggccactt tctgccagac ctgggacgac
    15601 gaaaatgtcc acaaattgat ggatttatcc attaa
//
LOCUS       DAJTYD010000081        15603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTYD010000081 DAJTYD010000000
VERSION     DAJTYD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.081"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1548
                     /gene="ldtD"
                     /locus_tag="OO585_002322"
     CDS             <1..1548
                     /gene="ldtD"
                     /locus_tag="OO585_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6629889.1"
                     /translation="RDMQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGV
                     NGMARDVVLSDAMMGYLHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQ
                     LPTFVAGLAPQHPQYAAMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQ
                     RTGMLDGGPKITLPGDDTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYD
                     NELVEAVKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTG
                     IMVNIPAYSLVYYQNGNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARK
                     DILPKVRNDPGYLESHGYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNS
                     LGRYKFNMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDK
                     RISDALKQGDTRYVNIRQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVS
                     KAEQLIR"
     gene            1729..2277
                     /locus_tag="OO585_002323"
     CDS             1729..2277
                     /locus_tag="OO585_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6629890.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2304..2951
                     /gene="gloC"
                     /locus_tag="OO585_002324"
     CDS             2304..2951
                     /gene="gloC"
                     /locus_tag="OO585_002324"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6629891.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3173..4363)
                     /gene="aspC"
                     /locus_tag="OO585_002325"
     CDS             complement(3173..4363)
                     /gene="aspC"
                     /locus_tag="OO585_002325"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6629892.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(4548..5636)
                     /gene="ompF"
                     /locus_tag="OO585_002326"
     CDS             complement(4548..5636)
                     /gene="ompF"
                     /locus_tag="OO585_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6629893.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYAHLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6238..7638)
                     /gene="asnS"
                     /locus_tag="OO585_002327"
     CDS             complement(6238..7638)
                     /gene="asnS"
                     /locus_tag="OO585_002327"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6629894.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7807..9009)
                     /gene="pncB"
                     /locus_tag="OO585_002328"
     CDS             complement(7807..9009)
                     /gene="pncB"
                     /locus_tag="OO585_002328"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6629895.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9275..11887
                     /gene="pepN"
                     /locus_tag="OO585_002329"
     CDS             9275..11887
                     /gene="pepN"
                     /locus_tag="OO585_002329"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6629896.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(11930..12681)
                     /gene="ssuB"
                     /locus_tag="OO585_002330"
                     /pseudo
     CDS             complement(11930..12681)
                     /gene="ssuB"
                     /locus_tag="OO585_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(12678..13415)
                     /gene="ssuC"
                     /locus_tag="OO585_002331"
                     /pseudo
     CDS             complement(12678..13415)
                     /gene="ssuC"
                     /locus_tag="OO585_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13480..14625)
                     /gene="ssuD"
                     /locus_tag="OO585_002332"
                     /pseudo
     CDS             complement(13480..14625)
                     /gene="ssuD"
                     /locus_tag="OO585_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(14622..15581)
                     /gene="ssuA"
                     /locus_tag="OO585_002333"
     CDS             complement(14622..15581)
                     /gene="ssuA"
                     /locus_tag="OO585_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6629897.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
BASE COUNT         3895 a         4069 c         3969 g         3670 t
ORIGIN      
        1 cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg
       61 gcagaggtgg cgattgccgg tttccagccg cagtttaata aatgggtaga gttactgacc
      121 gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat gatgggctat
      181 ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag cagtaaacct
      241 tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc gctggataaa
      301 ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata tgcggcgatg
      361 catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact gaccggcaaa
      421 gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa
      481 cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact
      541 gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac
      601 aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta cgataatgaa
      661 ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga tggtgctatt
      721 ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggct
      781 ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac
      841 attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga ttcgcgagtc
      901 attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa caacgtagtg
      961 gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct gccaaaagtg
     1021 cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgtgg ctggaacagc
     1081 agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc gaatttaccg
     1141 ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg
     1201 ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat
     1261 acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg
     1321 ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa gcagggtgat
     1381 acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt
     1441 gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg
     1501 cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc
     1561 ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc agcccccgtc
     1621 actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc
     1681 gccagaagtg catataaacg ataacattga cctgtagact tgattatcat ggacaaattc
     1741 gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg
     1801 ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat
     1861 cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa
     1921 gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc
     1981 gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg
     2041 gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg cgcccgcagc
     2101 cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa
     2161 ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga
     2221 tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagcaa
     2281 tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc gcattctccc
     2341 agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc gatcctggcg
     2401 gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc
     2461 tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg
     2521 gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc
     2581 aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg
     2641 aaggcgatac catcagcata ggaaatgtga ctttacaggt gttacattgc cctgggcata
     2701 cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta
     2761 ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt
     2821 cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt ccgggtcacg
     2881 gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa gacgaaatgc
     2941 ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt tctggcaaag
     3001 tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa aatcgtgcaa
     3061 attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag gcaatgttgc
     3121 gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat gcttacagca
     3181 ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg gccacattta
     3241 cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc acttgttctt
     3301 ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg aagtcgcggt
     3361 ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga atacgctggc
     3421 gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc aggatggtgg
     3481 caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc gccgctttca
     3541 tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta caagcgccaa
     3601 cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc agctctttat
     3661 gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg gcaaaaccct
     3721 ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt gccagtgtct
     3781 gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca tggaacagca
     3841 ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag tcaagagtgt
     3901 gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag ttaaagacgc
     3961 tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg gtattttttg
     4021 ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt gccatgcgag
     4081 cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga gtgcagcgac
     4141 caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt tcgagcagat
     4201 actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca tctttataga
     4261 caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga tcggccaggc
     4321 ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg ttccattatg
     4381 gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc caactattac
     4441 tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt ataaaaaaaa
     4501 caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta gaactggtaa
     4561 acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttatc agaatcgatc
     4621 tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta ggttgcgccc
     4681 acttcaaagt agttcaccag atcaacatca ccgatacctt ctacgtcttt cgctttagat
     4741 ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc aactaacaga
     4801 acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt gatcggcgta
     4861 gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc gtacttcaga
     4921 ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc ttgcaggttg
     4981 gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc gtagctgata
     5041 gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt tttacccagg
     5101 tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt acgataggta
     5161 gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc accaccaaat
     5221 tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt acggccgtaa
     5281 tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt tttgttacca
     5341 gtttgagcgt cagcgccttc agagttgtta ccttggaagt tatattccca ctgaccataa
     5401 ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagatgggc ataggtcatg
     5461 tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg cagaccaaca
     5521 gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc agcgtttgca
     5581 gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt catcattatt
     5641 tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt tctgtcaata
     5701 aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc
     5761 attcaaacta acgtttcgct accgtgaaag tgctgcaaag ataaagattt agtttcaaaa
     5821 agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg tcatatttcc
     5881 atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta cttatattta
     5941 aattaactaa gcggaaattt tgtttctgat ggaaacttta tcgacctggc acaaaatcct
     6001 ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag ctaagaaaaa
     6061 actaatccgc aataagaata cgggttattt tttgtgcgta aatgttgcaa tgtaatattc
     6121 cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta acaaacagat
     6181 aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg aagaaaatca
     6241 gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt tttgcacgcc
     6301 agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg gaacagtacc
     6361 gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca tttccagcat
     6421 acgctcgtcc agaacgtcca gacgttcttc acgctgggag cctccaatga tctcaccgat
     6481 gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga ggcgcatata
     6541 gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa agtgttcttc
     6601 cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg ggttttcaaa
     6661 cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct gcgcgaaatc
     6721 ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt cagcgaagaa
     6781 tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca gcatggcttc
     6841 agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca gcatccagaa
     6901 ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc cgaaggtata
     6961 aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata cggtcaggaa
     7021 agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt tacgcggcag
     7081 gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg tatcagatgc
     7141 ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc gatgcagcgc
     7201 ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc gcggacgcag
     7261 gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg ggtaagtgtc
     7321 tggatcttca acccaaccag caacttcaac tttgctggcc tgaatttcaa attgttgccc
     7381 ctggcccggc gacgccacga ctttacccgt cacaatgacc gagcagccgg tggtcagacg
     7441 caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta caggatcaaa
     7501 gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc ggcgggtacg
     7561 tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga gtacgtcggc
     7621 tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata aacacttgtc
     7681 cacccgtaaa tggggggata cctatgttac ctggcatctg caatcagaca agcagaattc
     7741 gcaaatgcag cgaaagattt cggaattaaa ggtaaaaaaa gggagctgat tagctccctg
     7801 atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca gcgcccgaac
     7861 aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg ccaccggttt
     7921 accgttacac tctaccaact taatcacaat attcaggggt tttacctggg ggatatcgca
     7981 ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt ggcggtatag
     8041 ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac tctgtggatc
     8101 aattcccagc tttttataat gtgcaatggc tttttcaccc cattcaaccg ggtcgccaga
     8161 gtcatgacgc aggccctgat accgactggc gaactcgaca ccgaaatcac gcaggaaagc
     8221 atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatatt cttccagcca
     8281 ggcagcaagt gcagctcgct ggctgttggc taggtccggg ctgatttgct gatgtgcctg
     8341 gaaccattcg tgcgcctgtg ttcccatcgg cgtgagggaa agccgacgcg ccagatcgta
     8401 gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg tttcttgtac
     8461 ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg acatatcaag
     8521 accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga gggcttgcgc
     8581 aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg ccagcaaagg
     8641 aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca gcttgccatt
     8701 atcgttggac acggtgactt gttccgggtt aaagcggaat tcgcgtaacc agttaagata
     8761 gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct gcaggcgcag
     8821 gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca gcagatcgtc
     8881 acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa acacggcttg
     8941 ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag gagaagcgaa
     9001 ttgtgtcata ggtgcgcagt agcatcctcg tacaggagcg tttagtacaa taaacatctt
     9061 caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca caccatatgc
     9121 cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca ataaaggcgt
     9181 ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga atagactgaa
     9241 caccagactc tataaaagat gctaaaggtt atttatgact caacagccac aagccaaata
     9301 ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga cctttgacct
     9361 cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg gtgcatcaga
     9421 tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata ttaatgatga
     9481 gccgtggacc gcctggaaag aagaagaggg cgcactggtc atcagtaatt tgccggagca
     9541 ttttacgctt aagatcgtta atgaaataag cccggcggcg aataccgcgc tggaagggct
     9601 ttatcagtca ggcgatgcgc tttgcaccca gtgtgaagcc gaaggtttcc gccatattac
     9661 gtattatctc gaccgcccgg acgtgctggc gcgttttacc accaaaatta ttgccgataa
     9721 aaccaaatat cccttcctgc tttccaacgg taaccgcgtt gcgcaaggtg aactggaaaa
     9781 cggacgccat tgggtacagt ggcaggaccc gttcccgaaa ccgtgctacc tgtttgcgct
     9841 ggtggcaggc gactttgatg tactgcgcga cacctttacc acgcgttctg gtcgcgaagt
     9901 ggcgctggag ctgtacgtcg atcgcggcaa ccttgatcgc gcgccgtggg cgatgacctc
     9961 gctgaaaaac tcaatgaaat gggatgaaga acgcttcggc ctggagtatg acctcgacat
    10021 ctatatgatc gtcgcggtgg atttcttcaa tatgggcgca atggagaata agggtttgaa
    10081 tatctttaac tccaaatatg tgctggcccg caccgacacc gccactgaca aagattacct
    10141 cgatattgaa cgcgttatcg gccatgaata tttccataac tggaccggta accgagtcac
    10201 ctgccgcgac tggttccagc tcagcctgaa agaaggttta accgtcttcc gcgatcagga
    10261 attcagctct gaccttggtt cccgtgcagt taaccgcatc aataatgtgc gcaccatgcg
    10321 cggattgcag tttgcggaag acgccagccc gatggcgcac ccgatccgcc cggacatggt
    10381 cattgagatg aacaacttct ataccctgac cgtttacgag aagggcgcgg aagtgatccg
    10441 tatgatccac accctgctgg gcgaagaaaa cttccagaaa gggatgcaac tctatttcga
    10501 gcgccatgat ggcagcgcgg caacctgcga cgactttgtg caggcgatgg aagatgcgtc
    10561 gaatgtcgat ctctcccatt tccgccgttg gtacagccag tccggcacgc cgattgtgac
    10621 cgtcaaagac gactacaatc cggaaaccga gcagtacacc ctgaccatca gccagcgcac
    10681 gccagccacg ccggatcagg cagaaaaaca gccgctgcat attccgtttg ccatcgaatt
    10741 gtatgacaac gaaggcaaag tgatctcgtt gcagaaaggt ggtcatccgg tgaattccgt
    10801 gctgaacgtc acccaggcgg aacagacctt tgtttttgat aatgtctact tccagccggt
    10861 gcctgcgctg ctgtgcgaat tctctgcgcc agtgaaactg gaatataagt ggagcgatca
    10921 gcaactgacc ttcctgatgc gtcatgcgcg taatgatttc tcccgctggg atgcggcaca
    10981 aagcctgctg gcaacctaca tcaagctgaa cgtcgcccga catcagcaag ggcagccgct
    11041 gtctctgccg gtacatgtgg ctgacgcttt ccgcgcagtg ctgctcgatg agaagattga
    11101 tccggcgctg gcggcagaaa tcctgacgct gccttctgtc aatgaaatgg ctgaactgtt
    11161 cgatatcatc gacccgattg ctattgccga agtacgcgaa gcactcactc gtactctggc
    11221 gactgaactg gcggatgagc tgctggctat ttacaacgcg aattaccaga gcgagtaccg
    11281 tgttgagcat gaagatattg cgaaacgcac tctgcgtaat gcctgcctgc gcttccttgc
    11341 ttttggtgaa acgcatctgg ctgacgtgct ggtgagcaag cagttccacg aagcgaacaa
    11401 tatgaccgat gcgctggcgg cgctttctgc ggcggttgcc gcacagctgc catgccgtga
    11461 tgcgctgatg caggaatacg acgataagtg gcatcaggac ggtctggtga tggataaatg
    11521 gtttatcctg caagccacca gcccggcggc gaatgtgctg gagacggtgc gcggtctgtt
    11581 gcagcatcgc tcatttacca tgagcaaccc gaaccgtatt cgttcgttga ttggcgcgtt
    11641 tgcgggcagc aatccggcag cgttccatgc cgaagatggg agtggttacc agttcctggt
    11701 ggaaatgctt accgacctca acagccgtaa cccgcaggtg gcttcacgtc tgattgaacc
    11761 gctgattcgc ctgaaacgtt atgatgccaa acgtcaggag aaaatgcgcg cggcgctgga
    11821 acagttgaaa gggctggaaa atctctctgg cgatctgtac gagaagatca ccaaagcact
    11881 ggcttgataa ataaccgaat ggcggcaata gcgccgccat tcggggaatt tacccctgtt
    11941 ttctcaggcg aatttcagat tcaccacgct gcatcaccct ttgcaacact tccgattcca
    12001 gctccgctaa tcgcaccgac cctaatcgac gcggaggggg gatatccact gtcaaatcca
    12061 gaccaatttt tccctcttca attaataaca cccggtcagc cattgctacc gcttcgctca
    12121 catcatgcgt caccaacagt acggtaaagc cgtgctcctg ccaaagtgac acaatcaaat
    12181 cctgcatctc gagtcgcgtt aaggcgtcca gtgccccgag cggttcatca agcaacaata
    12241 aacccggtcg atgaatcaac gctcttgcca gcaccactcg ctgtttctgc ccgcctgaaa
    12301 gtgcggcagg ccattccccg gcgcgattct ccagtcctac cgcagccaac gcccgacgcg
    12361 cggcatcgcg ccactggcct ttaaggccta acccaacgtt atcaatcacc gatttccacg
    12421 gtagcagacg cgcatcctga aacatcattc gcgtgtcttc ctgaatttca gccagcggtg
    12481 tggtgcctgc caacacatcg cctgcggttg gcgtttccag cccggccagc aggcgcagca
    12541 gggtactttt gccaccaccg ccacaaactg acctgccgga atatgtaaat ccagttggtt
    12601 caggacgata ttttccgcgt aatgtttgct tactgcattg agcaacaatg gtgtgccctg
    12661 gttcagacga gcagtattca taccgtggcc tccttcaaat gataagccgg gttccagcgc
    12721 aaccagaggc gctctaacag ctgcgcgctg acgtcagcaa gtttgccgag cagggcgtaa
    12781 agaataatag cgactaccac cacgtccgtt tgcaaaaact ctcgcgcatt catcgccaga
    12841 taaccaatgc cggaattggc agaaatggtt tcggcaacaa tcagcgtcag ccacatcagg
    12901 cccaacgcaa aacgcacgcc gaccataatt gagggcaggg caccaggcag gatcacatgg
    12961 ataaacagcg gtatgccgga taatccatag ctacgcgcca tctccaccag cccgcgatcg
    13021 atattacgga tgccatgcca ggtgttgata taaatgggaa acagcgtacc gagcgccacc
    13081 agaaagattt ttgcggattc atcaatgcca aaccacaaaa tcaccagcgg gatcagcgcc
    13141 agatgcggca cgttgcgcaa catctgaatt gaggtatcca gcagccgttc tccccagcga
    13201 gatagcccgc taatcagtcc caaaatcaac cccagcgatc cgccaattga aaagccaatc
    13261 agcgcccgct aggaactgat cgccagatgc tgccacagtt cgccgctggc ggagagcgtc
    13321 cagaacgccg tcaccacccc ttccggtgag ggcaaaatac gcgtcgacag ccagccaact
    13381 gaggaggcca gttgccacac cgccacgatg cccatcggta aaaactaggg ggcaacgcgc
    13441 aataaccact tcttcactgg cgttgccatt atttcttcct tagctttgcg cgactttacg
    13501 ggggataaaa tcattcgcca ccgcttcgcc ttgcggattc agcggctgcg gctggggaat
    13561 ttccggaatg gcgacatcca gatgtgggaa cagcaactcg ccgacccgat acgcttcttc
    13621 cagatgcgga tagcccgaaa gcacaaagct gtcgatgcca agcgcggcgt attcgttgat
    13681 tcgcgcagcg accgtaggac catcgcccac cagcgccgtt ccggcaccgc cgcgcactaa
    13741 gccaacgccc gcccataaat tggggctgat ctccagattg tcgcgcttgc cgttatgtaa
    13801 cgccgccatt cgctgttgtc ctacggaatc cgtccgggcg aatgcggcct gtgctttggc
    13861 gatagtttca tcatcaagat gcgagattaa ccgctcggcg gcctgccacg cttcgtcgtt
    13921 agtttcacga acaatcacat gcagacgaat accgaaacga attttgcgtc catgcgcggc
    13981 agctttcgcc cgcacttgtt cgatcttctc ttttactagt tctggcggtt cgccccaggt
    14041 gaggtagaga tcaacttgtt ctgccgccag ctcctgggcg acgtctgacg atccgccaaa
    14101 gtaaagcgga ggataaggct gttgaatcgg cgggaagagc agttttgctc cgcgcacatg
    14161 aatatgttta ccgttgaaat tgacggtttc ccccagcaat aagcgccgcc agacctaggt
    14221 aaattccgcc gaggcttcgt agcgctcgct atgatcaagg aacacgccgt cgcctgccag
    14281 ctcttgtgga tcgctgcctg tgaccagatt aaacaacgca cgaccatttg agagacggtc
    14341 aagcgtggcg gcctggcgag cggcaacggt aggtgaggtt acgctggggc gcagggcgac
    14401 aagaaacttc agccgctgcg tcaccgggat catcgacgcc gccaccagcc acgcatcttc
    14461 acacgatcgc cccgttggga tcagcacacc ggtatagccc agacgatccg ccgcttgcgc
    14521 aatttgttgc agataaccgt gatcaaccgg gcgtgaacct tcttccgttc ccagatagtg
    14581 cccgtcaccg tgggtcggta aaaaccagaa catattcaga ctcataattg ttttccttcc
    14641 agttgagtgg gctgccagat gcgctggcga atatcgactt ttttcggcac cagacgattt
    14701 tcataaaaca gatctgccgt ttgctgctgt aaggcggcaa cctcggcgtt aaccggtttg
    14761 atggtggtag gagggcgatg atctaagtaa gaggcaatca ccggtgccgg taagcccatc
    14821 gtttttgcca gtaaagcgat gctttgctcg cgctggctgc gggttaacgc atcggcctca
    14881 ctaaaggttg ccagtacgcc ctgaataaaa gcgccgtttt tttctgcata gggacgagct
    14941 gccagataaa acgatccagt ttgattgaga tcggtgccgt ctttcagcac ccgcacgccg
    15001 ccctgtaata atgcagcaga gtagtaggga tcccagatag cccaggcgtc aacgttacct
    15061 tgctggaacg cggcgcgggc atcagcgggc gtcaggtaag tgggttggat atcggtaaac
    15121 ttaagtccgg cctgacgcag tgcacgcagt aaaaggttgt gtgaactgga acctttctga
    15181 aaggcaactt tgtgaccttt aagatcggct acggttttga tcgggctgtt ttctgccacc
    15241 agaatcactt cggctttggg cttcggtggc tcgacgccca catataccaa atcagccccg
    15301 gcagcctggg caaagattgg cggaatatcc ccggtactgc cgagatcaat actgccaacg
    15361 tttaaggctt ccaacatttg cggacccgcg gggaactcca cccaggagat ttttgattgc
    15421 ggatagcgtt tttccagtaa ctggtggctt tttgccagta ccataccaat actgcctttc
    15481 tgatagccta tacataacgc ttcaggcgag gattctgcag caaccgcaat cgtagagacg
    15541 ctaagcaatc ccaccagcgc cagtttaatg atgttacgca tgggcattac ctcgcagaga
    15601 cag
//
LOCUS       DAJTYD010000082        15464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTYD010000082 DAJTYD010000000
VERSION     DAJTYD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.082"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..193
                     /locus_tag="OO585_002334"
     CDS             <1..193
                     /locus_tag="OO585_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6629898.1"
                     /translation="GELQKQLGIPGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAM
                     LEAIIEFLREECCVNSKIA"
     gene            complement(327..752)
                     /gene="arsC"
                     /locus_tag="OO585_002335"
     CDS             complement(327..752)
                     /gene="arsC"
                     /locus_tag="OO585_002335"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6629899.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(765..2054)
                     /gene="arsB"
                     /locus_tag="OO585_002336"
     CDS             complement(765..2054)
                     /gene="arsB"
                     /locus_tag="OO585_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6629900.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2109..2462)
                     /gene="arsR"
                     /locus_tag="OO585_002337"
     CDS             complement(2109..2462)
                     /gene="arsR"
                     /locus_tag="OO585_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6629901.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3201..3284
                     /locus_tag="OO585_002338"
     CDS             3201..3284
                     /locus_tag="OO585_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6629902.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3338..4690)
                     /gene="gorA"
                     /locus_tag="OO585_002339"
     CDS             complement(3338..4690)
                     /gene="gorA"
                     /locus_tag="OO585_002339"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6629903.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4762..5604)
                     /gene="rlmJ"
                     /locus_tag="OO585_002340"
     CDS             complement(4762..5604)
                     /gene="rlmJ"
                     /locus_tag="OO585_002340"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6629904.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            5807..7849
                     /gene="prlC"
                     /locus_tag="OO585_002341"
     CDS             5807..7849
                     /gene="prlC"
                     /locus_tag="OO585_002341"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6629905.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7857..8609
                     /gene="rsmJ"
                     /locus_tag="OO585_002342"
     CDS             7857..8609
                     /gene="rsmJ"
                     /locus_tag="OO585_002342"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6629906.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(8658..10127)
                     /gene="dtpB"
                     /locus_tag="OO585_002343"
     CDS             complement(8658..10127)
                     /gene="dtpB"
                     /locus_tag="OO585_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6629907.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10445..10879)
                     /gene="uspA"
                     /locus_tag="OO585_002344"
     CDS             complement(10445..10879)
                     /gene="uspA"
                     /locus_tag="OO585_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6629908.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11270..11605
                     /gene="uspB"
                     /locus_tag="OO585_002345"
     CDS             11270..11605
                     /gene="uspB"
                     /locus_tag="OO585_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6629909.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11849..13348)
                     /gene="pitA"
                     /locus_tag="OO585_002346"
     CDS             complement(11849..13348)
                     /gene="pitA"
                     /locus_tag="OO585_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6629910.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            13580..14782
                     /locus_tag="OO585_002347"
     CDS             13580..14782
                     /locus_tag="OO585_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6629911.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15097..>15464)
                     /locus_tag="OO585_002348"
     CDS             complement(15097..>15464)
                     /locus_tag="OO585_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HCR6629912.1"
                     /translation="VIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSK
                     VWLLALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGA
                     VCFTLFSIVSILEAGSAKK"
BASE COUNT         3906 a         3953 c         4063 g         3542 t
ORIGIN      
        1 cggtgagttg caaaagcagc ttgggatacc tggctccaca ctcagccacc atatatcagc
       61 actggtatcg gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga tgtgtgtttc
      121 gcaatacgca atgctggaag cgataattga attcttacgc gaggagtgct gcgtaaatag
      181 caaaattgcc tgatgcgcta cgctctacat atttcatgcc atatattgaa tttgcacggt
      241 tttgtaggtc ggataagacg cgttagcgtc gcatccgaca taaacaaagc gcactttacc
      301 aactatcggc agtggatatc gccgctttat ttcaggcgct tacccgcttc atcaaccact
      361 ttctcgccat cttccttgga gaatgcgcct ttttgcgcat ctggcagaat ttccagcacc
      421 acttctgaag ggcggcacag gcgagttccc agcggcgtca ccacaatcgg gcgattaatc
      481 agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt atcttccgca
      541 aggcccagct cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac ggaaatcccc
      601 atatcggcaa tgagtttgac cagttcatcg cgcgttggcg gagtttccag ataatggata
      661 atggtcggtt ctgtgccgct gttgcggatc atctccagcg tattacgcga cgtgccgcag
      721 gccgggttgt gataaatggt aatgttgctc atatcagtat ctcattacaa agtgaaagag
      781 agacgtagcg ccagcgcagc cagcgtcaca aacagcacag gcagggtcat gataatccct
      841 gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac gtgcagccag
      901 agtagtgtag ccaggctacc aattggggta attttcggtc ccaaatcgca gccaatcaca
      961 ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc aatggacaac
     1021 gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc ggtgaggaat
     1081 ccggtgccga gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag tacgccagaa
     1141 agatattccg ttaatccggc attgcgcagg ccataaacca ccagatacat gccgagcgag
     1201 aagatgacaa tttgccaggg ggcaccgcgc aggactttac ccgtattaat cgcatgaccg
     1261 cgtttagcga cgacaaataa tatcagcgcg cccacagctg caatggcgct caccggaatg
     1321 ccgagcggtt ccaggacgaa aaatcccacc agcagaagca gtaaaacaac ccagccagtt
     1381 ttgaacgtag caggatcttt gatcgcttct gcgggagatt tcagcagcgc catatcgtag
     1441 ttctgcggaa tatctttgcg aaaatagaga tgtagcatca ccagcgtggc aacaatcgcg
     1501 gcgatatcca ccggcaccat caccgaggcg tattcgcgaa agccgaggcc aaagaaatca
     1561 gcggaaacga tattcaccag gttggagaca ataagcggca ggctggcggt atcggcaatg
     1621 aatccggccg ccatcacgaa cgccagcgtc gtgcctttac tgaatcctaa agccagcagc
     1681 atggcgatga caatcggcgt caaaataagc gctgcgccat cattggcaaa cagggcggca
     1741 acggcagcac cgagcaggac aatccaggta aacagcaagc gaccacgacc attaccccag
     1801 cgtgagacgt gcagcgccgc ccattcaaaa aagccggact catccagcag caggctgatg
     1861 ataatgacgg cgataaacgc agccgtcgcg ttccagacga tattccacac caccggaata
     1921 tcacccggat ggaccacgcc cgtaactaac gctagtactg cgccgagcgt tgcactccag
     1981 ccgatgccta aacctttcgg ctgccagata accaatacaa tggtcaggac aaagatagcg
     2041 cctgccagta acataatgcc tcctggataa aacacatgcg aaaatacata tgtgtttagc
     2101 taaatttttt aactgcaaat gttcttactg tctgcggaac agttttgtcg agccaggttg
     2161 cggacaattg cctgaatctt ttcctgttca catcgccagg cttcatcaat tattttcgcc
     2221 gcccatgctg gaatatgcgg cgataagcgg taatgaaccc atttaccttg cttgcggtcc
     2281 agcaatagcc cgctttcacg cagcaatgcc aggtggcggg agatcttggg ctgcgactgg
     2341 tcgagagcag tgcagagatc gcagacgcat aactctccca gttcgctgag cagtaaaacg
     2401 atgcccagac gggtttcatc agcaagaatt ttgaacaatt ggatgggtaa cagaaatgac
     2461 atattgcgct cctgattgtt gcaggtagtg tctctcttcg aagcggataa gtcaaaaaca
     2521 tatatgattt aacgaatgtg taagtgcaga ggaaggtaat aggtgtgaat tttgagttgg
     2581 ctattcattt gaaaggaggt ctgaatcagg acgtaaaaaa accgactttg cgtcggtttt
     2641 ttactttcca gccctgagtt ggtggctctg gctggagtga gagagttctt atctaacagc
     2701 tcaatactat tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag
     2761 ggagcacttc ggtatatatc gcagtgacga tttatatact ttcactgggt catcgtcata
     2821 ttaagccttg tccgaatccg gatgtaaaaa accgactttg cgtcggtttt ttactttcca
     2881 gccctgagtt ggtggctctg actggagtga gatagccatc atctaacgca tcaatgctaa
     2941 taccatactg aaactattgc aaggacgtgc tggttttata acctgcatgt actgtatgat
     3001 tatccagtta gctctgaggc attttcactc tggcaatgcg cataaacgct ttcaaagtcc
     3061 tggtcagaag tacgggtggt gccgttaact gatgctctgg ccggagtgag agagttctta
     3121 tctaacaatg agacatgcgc cgtgacaggc agtggatgag taagcggatg cattctcact
     3181 ccatcgcatg gagaaaacgg gtgattgata aagcaatcat cgttctaggg gcgttaattg
     3241 cgctgctgga actgatccgc tttctgcttc agcttctgaa ctgatagcgg aaacgtaatt
     3301 aagggctaag agcacactac tcttagccct ttaacattta acgcattgtc acgaactctt
     3361 ctgccgccgt tgggtgaatg gcgacggtat tgtcgaagtc ttttttggtt gcccccatct
     3421 tcagcgccac cgcgaagccc tgcaacattt cgtccatacc aaagccaatg ccgtgaatac
     3481 cgacaatctt ctcttccgat ccaacgcaca ccagcttcat gcggcacggc tggcggtgag
     3541 tggtgacggc ggtatacatc gcggtgaaag aggatttata cactttcacc tgatcgtcgc
     3601 catactgctc gcgcgcctgc ggttccgtta aaccaacagt accaatcggc ggatggctga
     3661 agaccacggt cggaatgttg ctgtaatcca gatgctcatc cggcttgtta ttaaacaggt
     3721 gttcagagag acggcgaccc gctgcaactg ccaccggtgt cagctccact gcacccgtgt
     3781 tatcgcccac cgcgtaaata ccttcaatgt tggtgttttg atatttatcg acgacgatat
     3841 agcctttttc gttggtttta acgccagcgg cttcgaggtt gatgttgtca ttggcaggct
     3901 cgcgaccaat cgcccaaatc aggcaatcca ccgtttcact gcgaccatct tccagctcca
     3961 gcgtcaggct accatcggca tttttcacta ccgctttcgg gatggcgttg gtgtgcagct
     4021 gcgggccttc ggcgttcatc acttcgacca gcgtttcgga aatcatcggg tcgaagctgc
     4081 gcagcggcgc atgtttacgc acaaacagat gcgttttcgc gccgaggccg ttaatcacgc
     4141 ccgccagctc aacggcgatg taacccgcgc caacaaccgc cacgcgctct ggcaaagcag
     4201 gaagggcgaa gaagccatca gaatcaatac cgtattccac gcccggaata tccgggtggc
     4261 tcggacgacc gcctgtggcg atcagaatat gatcggccgt gatggtttcg ccgtttacct
     4321 ccagcatttt ggcatcaacg aagcgggcaa agcctttgat tacatcaacg ttatttttac
     4381 cgagcacgtt ttcataggaa gtatgaatac ggtcgatata ggcggtacgg ctggcgatca
     4441 acgtttccca gttgaacttg ttgatggtgg tatcgaaacc ataatcaggg ccgtacatat
     4501 ggatcgcttc acggatttgc gccgcgtgcc acatcacttt tttcggcacg cagccgacgt
     4561 tcacgcaggt gccgcccagc tctttggctt caatcagtgc gcatttctgg ccgtacatag
     4621 ccgcgcggtt gatggaggct ataccgccgc tgccgccgcc gatggcgatg taatcatagt
     4681 gtttagtcat gacagagtgt ccttatcgtt gattaccgcg attgtagcgc gagacgtaat
     4741 aggtgccagc aatggctgca attactccgg cacgatccag cttgcggtgg cgtgcccggt
     4801 gcctgccgga accagtttgc tgtgcagcca cggcagcacg ttattcatct gctgttccag
     4861 tttccacggc gggttaatca caatcatgcc ggaagcggtc atgccacggc gatcgctgtc
     4921 tggcagtacc gccagttcaa tttgcagaat tttgcgaata ccggtcgctt ccagatcgtg
     4981 gatcatgcgc ttaatttgct gacgcagcac caccggatac cacagtgcgt aagtaccagt
     5041 ggcgaaacgt ttgtaacctt ctgctatccc gctgaccacc gcctgatagt cggttttcat
     5101 ttcatacggc gggtcgataa ggattaaacc acggcgggaa accggcggca gtttggcctt
     5161 aagctgctgg aaaccatcgg ctttttcgac gcgcgcacgg ctgtctttct gaaattcaga
     5221 acgcagcaac gggtaatcgc tcgggtgcag ttcggtcagt tgcaggctgt cttgttcgcg
     5281 cagtagctgg cgagcaatca atggcgaacc ggggtagtaa cgcaactgac cgctacggtt
     5341 gaagtgtttt accacattga tgtacgcctc cagttctgcg ggcaaatcgt cctgctgcca
     5401 gatacgggcg atgccttcga gatattcgcc ggtacgctcg gcatgttcgc tgcctaactg
     5461 ataacgcccg gcccctgcgt gggtgtcgag atagagaaac ggtttatctt tctctttcag
     5521 cgactcgatg atcaggctct gaacggtatg tttaaggacg tcggcgtggt tgccagcgtg
     5581 aaagctgtgg cgataactga gcatgggtaa aggtgttccg gtaagtaaaa atcggcccgt
     5641 attcggggcg cacaaaagcg tatcaggaca gtataccgaa aagaggccgc cgccgcgaaa
     5701 gcgtaacgtt tctcattgaa attcactaca cttaacccca tgctacacac attatgtaaa
     5761 gcgcctgttg agcgcttcct taacctcttt aaccaggact gcgcgaatga cgaatccgtt
     5821 actgactccc tttgaattgc ctccgttttc taaaattctc ccggaacatg tcgttccagc
     5881 cgtgactcag gcgctgaacg attgccgcga aaatgtggag cgcgtagtag cgcaaggggc
     5941 accgtacacc tgggaaaatc tctgccagcc gttggcggaa gtggacgatg tgttggggcg
     6001 tatcttctcc ccggtcagcc acctgaactc ggtgaaaaat agcccggaac tgcgtgaagc
     6061 ctacgagcaa accctgccgc tgctgtcgga atacagcacc tgggtagggc aacatgaagg
     6121 gctgtataaa gcgtaccgcg acctgcgcga tggcgatcat tacgccacgc tgaacacggc
     6181 gcagaagaaa gcggttgata acgccctgcg tgattttgaa ctctctggca taggtctgcc
     6241 gaaagagaaa cagcagcgtt acggcgaaat tgcgacccgt ctttctgaac tgggcaacca
     6301 gtacagcaac aacgtcctcg atgcgacaat gggctggacc aaactcgtta ccgacgaagc
     6361 ggagctggcg gggatgcctg aaagcgcgct ggctgcggca aaagcccagg ccgaagcgaa
     6421 agagctggaa ggctacctgc tgacgctgga tatcccaagc tacctgccgg taatgaccta
     6481 ctgcgacaac caggccttgc gtgaagagat gtatcgcgct tacagcaccc gcgcctccga
     6541 tcaaggcccg aacgccggta aatgggataa cagcaaggtg atggaagaga tcctcgctct
     6601 gcgtcacgaa ctggcgcaac tgctgggctt tgaaaactac gcctttaaat cccttgccac
     6661 taaaatggca gaaaacccgc agcaggtgct ggatttctta accgatctgg caaaacgcgc
     6721 gcgtccgcaa ggcgaaaaag agctggcaca actgcgcgcc ttcgccaaag cggaatttgg
     6781 cgtcgatgag ttgcagccgt gggatatcgc gtactacagc gaaaaacaaa aacagcacct
     6841 ctacagcatc agtgacgaac agctgcgtcc gtacttcccg gaaaacaaag cggttaacgg
     6901 cctgtttgaa gtggtgaaac gtatttacgg catcaccgct aaagagcgta aagatgttga
     6961 tgtctggcat ccggatgtac gtttcttcga actgtatgac gagaacaacg aactgcgcgg
     7021 cagcttctac ctcgatctgt atgcccgtga aaacaagcgc ggcggggcgt ggatggatga
     7081 ctgcgtaggc cagatgcgta aagctgacgg ttctctgcaa aaaccggtcg cgtatttgac
     7141 ttgtaacttc aaccgcccgg taaatggtaa accggcgctg ttcactcacg acgaagtgat
     7201 caccctgttc cacgagttcg gtcacggcct gcaccatatg ctgacccgca tcgaaaccgc
     7261 tggtgtttcc ggtatcagcg gtgtgccgtg ggatgcggtc gaactgccga gtcagtttat
     7321 ggaaaactgg tgctgggagc cggaggcgct ggcgtttatc tctggtcact atgaaaccgg
     7381 cgaaccgctg ccgaaagagt tgctggataa aatgctggcg gcgaagaact accaggcggc
     7441 gctgtttatt ctgcgccagc tggagttcgg cctgttcgat ttccgcctcc atgccgagtt
     7501 ccgcccggat cagggtgcga aaatcctcga aactctggca gaaatcaaga aactggttgc
     7561 cgtagtaccg tctccatcct ggggccgttt cccgcacgct ttcagccata ttttcgccgg
     7621 tggttatgcc gcaggttact acagctacct gtgggctgac gtactggcgg cagatgcctt
     7681 ctcgcgcttt gaggaagagg gcattttcaa ccgtgaaacc gggcagtcgt tcctcgacaa
     7741 cattctgagc cgtggcggtt cagaagagcc gatggatctg ttcaaacgct tccgtggtcg
     7801 tgaaccgcag ctggatgcga tgctggagca ttacggcatt aagggctgat cattcagtga
     7861 aaatctgctt aattgatgaa acaggcgccg gagacggcgc cttatctgtt ctggcggccc
     7921 gctgggggct ggagcacgat gaagacaacc tgatggcgct ggtgttaacg ccggaacatc
     7981 tggaattgcg caagcgtgat gagccaaaac ttggcggcat ctttgttgat tttgttggcg
     8041 gagcgatggc gcaccgacgc aaattcggcg gtggtcgcgg tgaggcggtg gcgaaagcgg
     8101 tgggcattaa aggcgattat ttgccggatg tggtggacgc caccgcaggg ctggggcgcg
     8161 atgcctttgt actggcttca gtcggctgcc gcgtgcggat gctggagcgt aatccagtgg
     8221 ttgccgcgct actcgacgac ggcctggcgc gtggttatgc ggatgcggaa atcggcggct
     8281 ggttgcagga gcggttgcag ttaattcacg cctccagcct gacggcgcta accgatatta
     8341 ccccacgccc gcaggtggtc tatctcgatc cgatgttccc gcataagcag aaaagcgcgc
     8401 tagtgaaaaa agagatgcgt gtctttcagt cgctggtggg accggatctt gatgccgatg
     8461 gattactgga gcctgcacgc ttgctggcga ccaaacgcgt ggtggtaaag cgcccggact
     8521 acgcgccacc gctagcgaat gtcaccacgc caaacgcggt tgtgactaaa gggcatcgct
     8581 ttgatatcta tgcaggtacg cccgtgtaaa agtaaagcag ggtagcgata acgctaccct
     8641 gctttctgca agaataatta atggctttcc ggcgtcgcaa tcatgcgttt cagccacggc
     8701 accatcaaca gcatcactac tgcaacgccc agcgtgacca gaccaatctt accaaacacg
     8761 ttggtataga cgggcaacgt ctcaagcgga tcggtaatgt tgtccggcac cgcggtaaat
     8821 gttgccacat agccgcccag caagaacgcg gcagcctgcg tcaggaacca catcccgaga
     8881 ataaagccca tcaaatgctg cggcaccagg gcagcaatca tcgccaggcc aagggcgcta
     8941 ataaacagtt cacctaagct ctggaataag tacaccagca cgataaacca tggcgatgtc
     9001 agcccttgtg catccgcaaa ccacattccc gcagctgccg ccgtcaaaaa gcccagtgag
     9061 cacataaaca tgccgagagt aaatttcatc ggcatcgaga ggtctttgcc tttgttaccc
     9121 agatgcgtgt aaatgcctgc cagtattggg ctggcgagta ccacccagaa cgggttaagc
     9181 gcctggaagc tgaccgagtt gatggaaaaa ccgagaattt catgatgcac gttgttgatg
     9241 gcaaagaagt tcagcgatgt tggcatctgg gcgtagagaa tgtaaaacac caccgcttcg
     9301 agcatcagga caaaggcgac aaacatttta ttgcgcccgg ttttatccag cttgaatgcc
     9361 tgacgaaaga agatgatggt gacgacgatg gagagaacaa tcagcaccag attggcgact
     9421 tctacgttgt gcatcagcca tgcacatacg aagatcatca ccacgctgcc aagtaatacg
     9481 tacaacagtt tgctgaagct catcggcttg aagtcgggtt cagaaccaat gtctttcacc
     9541 attccacgac aggcgatgta aaccagtaat gcgataatta accccgcccc gcacaggttg
     9601 taggtgactg aataaccgaa tctatcagcg atcacagggg ccagcgataa cgctatcaac
     9661 gagccgatat tgatcgacat atagaacagg gtgaatgcgc catcaagccg cggatctttc
     9721 ggcggatagc acttcgaaag caagctggct gggttagctt taaacaggcc gttaccgaca
     9781 gcgatagtcc ccagggcgat gaaaatcagg tcaggcttaa gtagcgacat gccggtcatg
     9841 aagtagccaa tcgccagcac aagtgctccg agaacaatgg tgcgtttggt ccccagcagg
     9901 tggtcgccga catagccgcc aatggaaatg aggccataga ccagcgcagc aaaagcacca
     9961 aaagtgacaa aagcctgctc ttgcgagaat ccaagctgtt taacgaagaa aaccgccagt
    10021 acgccctgca cgccgtagta gccgaatcgc tcccataact cgacaaaaaa gatcatgaaa
    10081 aatgggcgag gttgctgcag catccccatg ggtgttgttg tattcatatt tattaacctt
    10141 ccaataccat cctgaaaagt atgaacgcgt aagggataac gcgcgaaaaa gtttacatct
    10201 gtcagagatt cttaatattg atgcggtgaa tggattaata cactattaca acagaaaata
    10261 accagctaat cacactaatg atggaaaata tgatggtgtt aaatgttgta ttggcaggag
    10321 attttgattg cgtacgcaga tcgtaggcca gataaggggt ttacgctgca tcaggtaaca
    10381 aggcgcgcca aaaagcaaaa cgcccgcata tgcgggcgtt caggaacacg tcgtggaggg
    10441 aagattattc ttcttcgtcg cgcagcggaa caatcagcat atcaacgtga acggtgttga
    10501 tcagctgacg tgcggaagac atcagtttgc tccagaagtc ctggtggtga ccacaaacca
    10561 ccagatccat atcgtatttc ttgattgcat cgaccagaac ctggcccagg tcgccgctgc
    10621 cgctcagggt ttcagtgatt gggtagcctg cattagtgga aagctcggtc agcgcatgat
    10681 gtgtctcttc agagatgcgt ttctgcatat cacccagatt cacatcaata agcccggtgt
    10741 ataggtcaga gtagtttaca tctacgtgga tcagagaaac tttcgcattg taggggcgag
    10801 ccatagagac tgctttctct accagaactt tgctttccgg ggagagatcg accgcgatga
    10861 gaatgtgttt ataagccata gtgttactcc ttccataaag ttgtcgatga ctggccagct
    10921 agcgtttctt gtgccgttac gtcacccgcg tcctgcgaac tacgtgcgcg gggctaaccc
    10981 agccgtaaag attcagtgtt aagaccatcc ttaccttata gcgacccgga tgatccgtca
    11041 atccgccttg cttacccgtt agataaacaa attttcgaca tgaatatatt gatagtggtt
    11101 aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg ctcggatgtt
    11161 ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag cgtatcggga
    11221 gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta tgataagcac
    11281 cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc
    11341 atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct atcaatatgt
    11401 tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg
    11461 gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc gccgctgtga
    11521 gcgggtgcgt cggcagttta ttctgactag cgcattgtgt ggtctggtgg tggtcagcct
    11581 gattgcattg atgatttggc attaagcact tcagcaataa taacgcataa aaaaagctgg
    11641 ccagtaaact gacccgctcg agactgatta caaacgcaaa attgcctgat gcgctacgct
    11701 tatcaggcct gcataatctc tgcaatctat tgaatttgca tgtttttgta ggccgaataa
    11761 ggcgttcacg ccgcatcagg catgaacaaa gcgcactatg tcacaatctg aagcgggcca
    11821 gctgacccgc tcgttttggt gcgtacgatt acaggaactg caaggagagc cagtacagcc
    11881 cgccggaaag cagtaccgca gcaggaaggg taaacaccca ggccatcaga atgctggtca
    11941 cggttttacg ctgtaagccg ccaccatcta ccaccatcgt ccccgcgaca gaagaggaga
    12001 gtacgtgagt agtggaaacc ggcatcccgg tataactcgc caggccgata gacactgccg
    12061 ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg attttctcac
    12121 cgatagtcgt tgccacacga cgccagccaa tcatcgtacc gatacctaac gccagcgcga
    12181 ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg gacttcagtt
    12241 tcttcaacag gcgctgatcg tcagcactca cgccaggtat cttcactact ttgtcgatag
    12301 tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc gacagcttgt
    12361 cgtagctttc cacatcggta gttaacatac ctttcaggcg gttgagcgcg ttaatggtat
    12421 tcgacggatg gcaatggaac tccgcgggtt gcgttgcgcc agcttccgga gccggtacta
    12481 actgatcagc accggtagcc tgtttcagca gcgcaggatg ctgctcaaag taagcttcga
    12541 cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc atattcacca
    12601 cgaagcctgc tggtgcgaca ccaatcaata ccaacataac cagaccaatg cctttctgac
    12661 catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc gcaatacgcg
    12721 tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg gtcaggtgga
    12781 tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc agaccgccag
    12841 caaacaccag gccgacaata ggggaaacaa tcagagaacc gaaaatactt aatactttcg
    12901 ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt aaaccaatcc
    12961 cgatgatcgc gccaatcagt gtatgagagc tggatgcagg taaaccaaag taccaggtac
    13021 ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg ccatgagacg
    13081 atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag gcaacactca
    13141 gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg gcgagctgag
    13201 aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca tggaaaccat
    13261 tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc ccggtatgca
    13321 aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg acatgaacgc
    13381 ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt tttttgattt
    13441 gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg caaatacttc
    13501 cttttagtaa tattgatgct ggtgtgacca ctgaggaatc tttacaattc acgctcgttt
    13561 tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg ctggtgcggc
    13621 gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc tgatcgataa
    13681 tggtaaaaaa ccagggcgca aaatccttat gtctggcggt gggcgctgca actttaccaa
    13741 cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta agtctgcact
    13801 cgcgcgtttt acccagtggg atttcattga tctggtcaat aaacacggca tcgcctggca
    13861 cgagaaaacg ttaggacaac tcttctgcga tgactccgcg cagcagattg tcgacatgct
    13921 ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag tgctgagtgt
    13981 ggcgaaggat gaaacaggct tcacgcttga actgaacggc atgactgtcg gttgcgaaaa
    14041 gctggtcatc gcgaccggtg ggctgtcaat gccggggctg ggcgcatcgc cgtttggtta
    14101 taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgtgcgggtc tggtgccatt
    14161 cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg cggtgccttc
    14221 cgtgattacc gctgaaaacg gcaccgtttt ccgtgagaac ttactcttca cccaccgcgg
    14281 cttatctgga ccggcggtgt tgcagatttc cagctactgg caaccgggtg aatttgtcag
    14341 tatcaatctg ctaccggatg tggacctcga aaccttcctg aatgagcagc gtaacgcaca
    14401 tccgaatcag agcctgaaaa acacactggc ggttcatcta ccgaagcggt tggttgaacg
    14461 cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg tgcgtgacca
    14521 acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca ctgaaggcta
    14581 tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactctctt cacggacgat
    14641 ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg ttaccggctg
    14701 gctggggggc tataacttcc agtgggcgtg gtcgagtgcg tgggcttgtg cgcaggattt
    14761 gattgcagcg aagtcgtcct gacctgttac ctaaagatga aaattattag agccgcatta
    14821 aaaatgagct aattttgata gtggctatct tgtgattatt ttctaatgag cccgtgaact
    14881 gaaaccctcc aggcttaata taaggtggaa ggaaagatga ttgaaaactc acccagtggc
    14941 cagatcttta taaaatatga acaggctgaa aaagacagta actttgttgt tttttatcct
    15001 taaccactat gcattaatgc tgcgttattt catgatgcct aagaaaaacc agcgttacgc
    15061 aaatggtcaa cgctggtttt atccggtacg ttgcaattat tttttagcag aacctgcttc
    15121 taatattgaa acgattgaga acaacgtaaa gcataccgct cccaaaccga ccaaaacccg
    15181 cgaaggaata aaatatccca tgtcggtctg cgccatttcc gcaagaaacg atgccaggaa
    15241 aaggcaaaac aggcaggtga agacgggaat catcggtatt ctgttggcta acgaacaggt
    15301 acgtctccat accagtgcca gtagccagac ttttgagaat atgctgtaac agatcattcc
    15361 gaggtaaata agaataatgc cgggagccag tcgggcgctt gcatcggaac tgactaagac
    15421 gtatatcccc tgcagtaccg tgattgagcc gagaaaaata accc
//
LOCUS       DAJTYD010000083        15370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTYD010000083 DAJTYD010000000
VERSION     DAJTYD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.083"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..226
                     /locus_tag="OO585_002349"
     CDS             <1..226
                     /locus_tag="OO585_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HCR6629913.1"
                     /translation="RDALMEALKERGIGTGLHFRAAHTQKYYRERFPTLSLPNTEWNS
                     ERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            230..1198
                     /gene="arnC"
                     /locus_tag="OO585_002350"
     CDS             230..1198
                     /gene="arnC"
                     /locus_tag="OO585_002350"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6629914.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            1198..3180
                     /gene="arnA"
                     /locus_tag="OO585_002351"
     CDS             1198..3180
                     /gene="arnA"
                     /locus_tag="OO585_002351"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6629915.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            3177..4067
                     /locus_tag="OO585_002352"
     CDS             3177..4067
                     /locus_tag="OO585_002352"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6629916.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            4067..5719
                     /gene="arnT"
                     /locus_tag="OO585_002353"
     CDS             4067..5719
                     /gene="arnT"
                     /locus_tag="OO585_002353"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6629917.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            5716..6051
                     /gene="arnE"
                     /locus_tag="OO585_002354"
     CDS             5716..6051
                     /gene="arnE"
                     /locus_tag="OO585_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6629918.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            6051..6437
                     /gene="arnF"
                     /locus_tag="OO585_002355"
     CDS             6051..6437
                     /gene="arnF"
                     /locus_tag="OO585_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6629919.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(6431..6697)
                     /gene="pmrD"
                     /locus_tag="OO585_002356"
     CDS             complement(6431..6697)
                     /gene="pmrD"
                     /locus_tag="OO585_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6629920.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(6807..8162)
                     /gene="menE"
                     /locus_tag="OO585_002357"
     CDS             complement(6807..8162)
                     /gene="menE"
                     /locus_tag="OO585_002357"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6629921.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(8159..9121)
                     /gene="menC"
                     /locus_tag="OO585_002358"
     CDS             complement(8159..9121)
                     /gene="menC"
                     /locus_tag="OO585_002358"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6629922.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(9121..9978)
                     /gene="menB"
                     /locus_tag="OO585_002359"
     CDS             complement(9121..9978)
                     /gene="menB"
                     /locus_tag="OO585_002359"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6629923.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(9993..10751)
                     /gene="menH"
                     /locus_tag="OO585_002360"
     CDS             complement(9993..10751)
                     /gene="menH"
                     /locus_tag="OO585_002360"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6629924.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(10748..12418)
                     /gene="menD"
                     /locus_tag="OO585_002361"
     CDS             complement(10748..12418)
                     /gene="menD"
                     /locus_tag="OO585_002361"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6629925.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(12507..13802)
                     /gene="menF"
                     /locus_tag="OO585_002362"
     CDS             complement(12507..13802)
                     /gene="menF"
                     /locus_tag="OO585_002362"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6629926.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(13881..14186)
                     /gene="elaB"
                     /locus_tag="OO585_002363"
     CDS             complement(13881..14186)
                     /gene="elaB"
                     /locus_tag="OO585_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6629927.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(14241..14702)
                     /locus_tag="OO585_002364"
     CDS             complement(14241..14702)
                     /locus_tag="OO585_002364"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6629928.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            14767..>15370
                     /gene="rnz"
                     /locus_tag="OO585_002365"
     CDS             14767..>15370
                     /gene="rnz"
                     /locus_tag="OO585_002365"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCR6629929.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAP"
BASE COUNT         3527 a         4185 c         3951 g         3707 t
ORIGIN      
        1 tcgcgatgcg ttgatggaag cgttaaaaga aagaggcatt ggtaccgggt tacatttccg
       61 cgccgctcac acacaaaaat attatcgcga gcgttttccc acgctgtcgt taccgaatac
      121 cgaatggaat agcgaacgca tctgttcttt gccgctgttc ccggatatga ctaccgccga
      181 tgccgaccgt gtcatcacag cccttcagca gctcgcagga caataagcca tgtttgaaat
      241 ccaccctgtt aagaaagtct cggtggttat tcccgtttat aacgagcagg aaagcttacc
      301 ggaattaatc aggcgcacca ccacagcctg tgaatcgttg gggaaagagt atgagatcct
      361 gctgattgat gacggcagta gcgataattc cgcgcatatg ctggtcgaag cctcacaagc
      421 ggagaacagc catattgtgt ctattttgct taaccgcaat tacgggcaac attcagcgat
      481 tatggcggga ttcagtcacg ttactggcga cttaattatt acccttgatg ccgatctcca
      541 gaatccgcca gaagaaatcc cccgcctagt ggcaaaagcc gatgaaggtt acgacgtggt
      601 agggactgta cgccagaacc gccaggacag ctggtttcgt aaaacagctt cgaagatgat
      661 taaccggctt attcagcgca ccaccggcaa agcgatgggt gactacggtt gtatgctgcg
      721 cgcctatcgc cgtcatattg tcgatgcgat gttgcactgc catgaacgta gcacctttat
      781 cccgattctg gcgaatatct tcgcccgccg tgccattgaa attccagtac atcatgccga
      841 gcgtgagttt ggtgaatcca aatacagttt tatgcgcctg attaatttga tgtacgacct
      901 ggtgacctgc cttaccacaa cgccgctacg tatgctgagt ctgctcggca gcattattgc
      961 gattggaggt tttagcattg cggtgctgct ggtgatttta cgcctgacct tcggaccaca
     1021 atgggcggca gaaggcgtct ttatgctatt tgccgtgctg tttactttta ttggcgctca
     1081 gtttatcggc atgggattac tcggtgaata tatcggcagg atctacaccg atgtccgcgc
     1141 ccgcccccgc tattttgttc agcaagttat ccgtccatcc agcaaggaaa atgaataatg
     1201 aaaaccgtcg tttttgccta ccacgatatg ggatgcctcg gtattgaagc tctgctggct
     1261 gccggttacg aaattagcgc catttttacc cataccgata atcccggtga aaaagccttt
     1321 tatggttcgg tggctcatct ggcggcggaa agaggcattc cggtttatgc gccggataac
     1381 gttaatcatc cgctgtgggt ggaacgcatt gcccaactgt caccagaggt gattttctct
     1441 ttttattatc gccatcttat ttgcgacgag attttgcagc tcgctcccgc aggtgcattt
     1501 aatctgcacg gttcgctgtt accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg
     1561 gtgaacggtg aaacggaaac gggcgttaca ttgcaccgaa tggtaaaacg tgccgatgct
     1621 ggggccattg tggcgcaact gcgcgttgcc attgcgccag acgatatcgc tattacgctg
     1681 catcataaat tgtgccatgc cacgcgccag ctactggaac agacattacc cgccattaaa
     1741 cacggtaata ttctggaaat cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga
     1801 acgccggatg acagtttcct tgaatggcat aaaccggcat ccgtactgca caacatggta
     1861 cgtgccgttg ccgatccgtg gccgggtgcc ttcagctatg ttggcaatca gaaattcacc
     1921 gtctggtcgt cgcgtgttca tcctcatgcc agcaaagcac agccggggag cgtgatttct
     1981 attgcaccgc tgttgattgc ctgtggcgat ggcgcgctgg aaatcgtcac cggacaggcg
     2041 ggcgacggca ttactatgca gggctcgcaa ttagcgcaga cgctgggcct ggtgcaaggt
     2101 tcacgcttga atagccagcc tgcctgcacc gcccgacgcc gtacccgggt actcatcctc
     2161 ggggtgaatg gctttattgg caaccatctg acagaacgcc tgctgcgcga agatcattat
     2221 gaagtttacg gtctggatat tggcagcgat gcgataagcc gttttctgaa tcatccgcat
     2281 tttcactttg ttgaaggcga tatcagtatt cattccgaat ggattgagta tcatgtcaaa
     2341 aaatgtgatg tcgtcttgcc gctggtggcg atagccacgc cgattgaata tacccgcaac
     2401 ccgctgcgcg tatttgaact cgattttgaa gagaatctgc gcattatccg ctactgcgtg
     2461 aagtaccgta agcgaatcat cttcccgtca acttcagaag tttatgggat gtgtagcgat
     2521 aaatacttcg atgaggacca ttctaattta atcgtcggcc cggtgaataa accacgctgg
     2581 atttattcgg tatcaaaaca attacttgat cgggtgatct gggcctatgg cgaaaaagag
     2641 ggtttacagt tcaccctctt ccgcccgttt aactggatgg gaccacgact ggataacctt
     2701 aatgcggcac gaatcggcag ctcccgcgct attacgcaac tcattctcaa tctggtagaa
     2761 ggttcaccga ttaagctgat tgatggcgga aaacaaaaac gctgctttac tgatattcgc
     2821 gatggtatcg aggcgttata ccgcattatc gaaaatgcgg gaaatcgctg cgacggtgaa
     2881 attatcaaca ttggcaatcc tgagaacgaa gcgagcattg aggaactggg cgagatgctg
     2941 ctggcgagct tcgaaaaaca tccgctgcgc catcatttcc caccgtttgc gggctttcgc
     3001 gttgtcgaaa gtagcagcta ctacggcaaa ggatatcagg acgtagagca tcgtaaaccg
     3061 agcatccgca atgcccaccg ctgcctggac tgggagccga aaattgatat gcaggaaacc
     3121 atcgacgaaa cgctggattt cttcctgcgc accgttgatc ttacggataa accatcatga
     3181 ccaaagtagg cttacgcatt gatgtcgata cctttcgtgg cacccgtgaa ggcgtgccgc
     3241 gtctgctgga aatcttgagt aagcataata ttcaggccag catttttttc agcgtcggcc
     3301 cggacaatat gggccgccat ctctggcgac tggtgaagcc acagtttttg tggaagatgc
     3361 tgcgctcaaa cgcggcatcg ctttatggct gggatatttt actggcaggt acggcctggc
     3421 caggtaaaga gattggtcat gccaatgccg atatcattcg tgaagcggct aaacatcacg
     3481 aagtcggcct gcacgcctgg gatcaccatg cctggcaagc ccgtagcggt aactgggatc
     3541 ggcaaacaat gatcgacgat attgcacgcg gtcttcgcac tctggaagag attatcggtc
     3601 aaccggtaac ctgttctgcc gctgcgggct ggcgtgccga ccagaaggtg atcgaagcaa
     3661 aagaagcgtt ccatttgcgc tacaacagcg attgtcgtgg ggccatgccg ttccgtccat
     3721 tgctcgaatc aggaaaccct ggcactgcgc aaattccggt gaccttaccc acctgggatg
     3781 aagtgattgg tcgggatgtg aaaacagaag attttaacgg ttggttactc aaccgcatcc
     3841 tgcgagataa aggcacgccg gtttatacca ttcatgcaga agttgaaggc tgcgcttatc
     3901 agcataattt tgtggatctc ctcaaacgcg cagctcagga aggcgtgaca ttttgccctt
     3961 taagcgaact gttatcagag acgttgccgc tcggacaagt tgttcgcgga aatattgccg
     4021 gacgtgaagg ctggctgggt tgccaacaaa ttgcgggtag tcgctgatga aatcggtacg
     4081 ttaccttatc ggcctcttcg cgtttattgc ctgctattac ctgttaccga tcagcacgcg
     4141 tctgctctgg caacccgatg aaacgcgtta tgcggaaatc agtcgagaaa tgctagcatc
     4201 cggcgactgg attgtgcccc atctgttagg gctacgttat ttcgaaaaac ccattgccgg
     4261 atactggatt aacagcattg ggcaatggct atttggcgcg aataactttg gtgtgcgggc
     4321 aggcgttatc tttgcgaccc tgttaactgc cgcgctggtg acctggttta ctctgcgctt
     4381 atggcgcgat aaacgtctgg ctctactcgc cacagtaatt tatctctcat tgtttattgt
     4441 ctatgccatc ggcacttatg ccgtgctcga tccgtttatt gcattctggc tggtggcggg
     4501 aatgtgcagc ttctggctgg caatgcaggc acagacgtgg aaaggcaaaa gcgcaggatt
     4561 tttactgctg ggaatcacct gcggcatggg ggtgatgacc aaaggttttc tcgcccttgc
     4621 cgtgccggta ttaagcgtgc tgccatgggt agcgacgcaa aaacgctgga aagatctctt
     4681 tatttacggc tggctggcgg ttatcagttg cgtactgacg gttctccctt ggggactggc
     4741 gatagcgcag cgggagcctg acttctggca ctattttttc tgggttgagc atattcaacg
     4801 ctttgcactg gatgatgccc aacatagagc tccgttctgg tactacgtgc cggtcatcat
     4861 tgccggtagc ctgccgtggc tgggattact ccccggtgca ctgtacacag gctggaaaaa
     4921 ccgcaagcat tccgcaaccg tctatttgtt gagctggacg ataatgccgc tgctgttttt
     4981 ctccgtcgct aaaggtaaat tgcccaccta tattctttcc tgctttgcat ctctggcaat
     5041 gctgatggcg cattacgctt tgctggcagc aaaaaataat cctctggcgc tgcggattaa
     5101 tggctggatt aacatcgctt ttggcgtcac tggcattatt gccacatttg tggtctcccc
     5161 gtggggacca atgaacacgc cggtgtggca aaccttcgag agctataaag tcttttgtgc
     5221 ctggtcgatt ttttcgctat gggcattttt cggctggtac accttaacaa acgtcgaaaa
     5281 gacctggtct ttcgccgcgc tttgcccgct ggggctggcg ttgctggtag gattttcaat
     5341 tcctgacaga gtcatggaag gaaaacatcc gcaatttttt gtcgagatga cacaagaatc
     5401 acttcaacca agccgctata ttcttactga tagcgtcggt gttgccgcag gtctggcatg
     5461 gagcctgcaa cgcgatgaca tcatcatgta tcgccagaca ggtgagttga aatacggcct
     5521 taattatccg gatgcgaaag ggagatttgt cagcggtgat gagttcgcaa actggcttaa
     5581 tcaacatcgt caggagggga ttattacact cgtgctttcg gttgaccgcg atgaagatat
     5641 caacagtctc gccattccgc ccgcagatgc catcgatcgt caggagcgtc tggtgctgat
     5701 tcagtatcgt cccaaatgat ctggctaaca ttagtctttg ccagcttgct tagcgttgcc
     5761 gggcagttgt gtcagaaaca ggcaacctgc tttgtggcga taaacaaacg gcgcaaacat
     5821 atcgtgctgt ggctgggact ggcgctggct tgtcttggtc ttgccatggt gctctggctg
     5881 ctggtcttgc agaacgtacc ggtaggcatt gcctacccga tgttaagtct gaattttgtc
     5941 tgggtgacgc tggctgcagt aaaactgtgg cacgaaccgg tatcgccgcg tcactggtgt
     6001 ggggtggcgt tcattattgg tggcattgtg atcctcggga gtacggtgta atgggcctga
     6061 tatggggatt attcagcgtc attattgcct cagttgcgca attaagcctg ggttttgcgg
     6121 caagtcatct gccgccgatg acgcacttat gggattttat cgcggctctg ttagcttttg
     6181 gcctggatgc ccggatactg ttactcgggc tgctgggtta tctgctttcc gttttttgtt
     6241 ggtataaaac gctacataaa cttgcgctaa gcaaagccta tgccttgctc agtatgagtt
     6301 atgtgctggt atggattgct tcaatggtac ttcccggctg ggaagggact ttttcgctga
     6361 aagcactact gggagtagcc tgtattatga gcgggttgat gctgattttc ctgcccacga
     6421 caaaacaacg ttactgagtt ttccctgccg ctgtacactg ccgttcccac tcatcggggc
     6481 tataactgct tgccgagagg actttcaccg tatgcagctt ttcacgatta atacaataaa
     6541 gcgcattctg caaaggcgag agtaaatccc ccactttcac ggcgaagtca ctcttcactt
     6601 cggcgatcat ttttatcgcg ccgccagcat cgcagagcat aagtacatgt ctgttatctt
     6661 gtttattgca gcacgatttt ttgaccagcc attccattgc attatcctgt ttgctaagag
     6721 ttttcacatc aatttacaga ttaacagata aactctttat caacggcaat ggaatggcag
     6781 aaatgagaag tgaaacggag ggttaattat tgctgacgtt gcacccactc ttttagcgcc
     6841 tgacgtgaaa ttttaatacc gccgtttttc agctccggcg gtaaagtcag ccagcgcacc
     6901 ggttgttgaa aacgagccag cttatctttc acccattcgc caagatcaac ggtttgctgg
     6961 tcatactcca ccaccgctac cggccgatga ccaaactcct tgtcggcaac gggaacgata
     7021 aacacctgca aaaccgcagg atgtgcagca ataacacgct cgacttcttc cggctgaata
     7081 ccctcaccgc cactgaataa cagattgtct aaacgtccga caatggtcag cttgccatta
     7141 tgcatctcac cgcgatcgcg cgtagcgtac cagccttcgt cattaaccag tgaaaccagt
     7201 tgcccgttac gccagtaacc ttctgccata ctggcagccc gcagccacac ttcattatta
     7261 acgattttca cttcccgacc cggcagcggc gaaccaacgt ctgccaggcc gtcggcttct
     7321 ttcgcacata ccgtggaggc aaactcggtc agaccatagc cgcaaaagca acgaatcccc
     7381 tgctcgcgcg cctgttccgt caactcgacc gggatagccg cgccgccaag taacaccgct
     7441 ttcagggaaa cggaactgcg gttaaccagc aaacgccaga gttgtgttgg caccagtgaa
     7501 gcgtgagtac agcctgccag catttgctcc aatggctgtt tatcacgcac cgtcatccgc
     7561 gcaccagcgt ataaccagcg ccacataatt ccctgaccgg agacgtgaaa cagcggtaaa
     7621 gagagcaacc agtcatcgtg atcgccaaac ggaatcagcg ataacacacc ttgcgcactg
     7681 gcaagatggg cctgataagt atggacagcg gctttcggta aaccggtgga gccggaggtc
     7741 aatgtcattg aacaaagacg tgttggttgc cacagagctg aatgtgtgcc ttcaaccatc
     7801 tgaatatgca gtgaagttaa cgccgggaaa gtattttctc cttccagcac cagcgcatat
     7861 tgcagtgtaa gatttggcag taattcctca agcaacggtt gcggaagctg tggatttacg
     7921 ggcagcaccc gcgccccgca ttgcagtaac gccagccagg cgagcagcgt ttgcggcgta
     7981 ttccacgccc gcaacatcac gccgctgccc tcaaccacac cctgcgccgc aaagcccgta
     8041 gctaactcat cgacgcgagc acaaagctcg cgccagttga gttgctcgtc attaagacgt
     8101 aaggcgatgg cttctccccg cacttgccgc cagtgacgcc acggccagtc agagaagatc
     8161 ataacaaccg ctccagtgca tcaacttcca cgacaggcag cgtgctaccc ggccagcgac
     8221 gtacctgctg cgcctgcatc agatccagcg tgtccagccc tggaatggtg tccggcgtta
     8281 accaggcggc aatccgcgcc agttgcgtta agcctaagct cgactcaata gaagaactga
     8341 tcaccgccgt cagcctcaac gcgtgcgccg cctgtacctg ctcgcgtact ttttccagac
     8401 tgcccgtgag cgtgggtttg ataactaccg cgcgcacgcc ctcttcagcc acaaaggcaa
     8461 aatccggctc gcgcaggctt tcatcccagg caatggcaat gccggtttca cgggcaaacg
     8521 ctcgcgaatc atcgcgggtt ttgcacggct cttcgagaaa cgcgatgcgg tggcgataat
     8581 ccgggttaac atatttggca aactgctgac ctttcagcgg tgtccaggcg cgatttgcgt
     8641 caagacgcaa atgcagatcc ggaattgcct ccagcaacag attaaccacc atgccgtcgc
     8701 gcacggcttc gtacaatccc actttgacct tcgccacttt ctcgcctggc atatcagcaa
     8761 gtttgaggat cagatcgtca ggatcgccat tacacagtgg tgccgcacgg taattggccg
     8821 cctgcgggag cgtatctgcc agctccgcca atgcacagct tacgccaaag gcaacggaag
     8881 gcatctgcag tagctcgcaa tcgcctgcca gccagttatt tacccaggca agcagcacgc
     8941 tttgcgcctc ttcccaggtt tcctggctga agcccggcag tggggagatc tccccccacc
     9001 cttcgcgctc gccctcacgc aggcaaacgt acagcccgtc gcgggttttt aaccgcctgt
     9061 cgcgcagaac cacccccgcg tccatgggga tctgccagcg gtatacctgc gcgctacgca
     9121 ttacggattc cgtttaaatt tgctgaaatc aggctgacgt ttctggttga aagcgttgcg
     9181 accttcctga ccttcttccg tcatgtagaa cagcatggtg gcgttgcccg ccagctcctg
     9241 cagccccgcc tgcccgtcac agtcggcgtt cagtgcagct ttcaggcagc gcagcgccat
     9301 cggactgttt tgcagcattt cgcggcacca gcgaacggtc tctttttcca gatccgccag
     9361 cggtaccacg gtgttgacaa ggcccatatc cagcgcctgt ttcgcgtcgt actgacggca
     9421 caggaaccag atttcccgcg cttttttctg tccgacgatg cgagccatgt aggaagcgcc
     9481 ccagccgccg tcgaaggaac cgactttcgg gccagtctga ccgaagatgg cattatctgc
     9541 cgcgatagtc aggtcgcaca tcatgtgcag aacgtgaccg ccgccgatgg agtagccagc
     9601 caccatcgcg acaaccggtt tcggacaggt acggatctga cgctggaagt ccagcacgtt
     9661 caggtgatgt acgccggaat catctttata gccgccgtaa tcaccacgca ctttctggtc
     9721 accgccggag cagaacgctt tatcacctgc gccagtcaga atgatcacgc cgacgttgtc
     9781 gtcataacgc gcatctgcca gtgcctggat catctctttt accgtcagag gacggaaggc
     9841 attgcgcacc tgcggacgat taatggtgat ttttgcgata ccatcggtgg atttttcata
     9901 acgaatgtcc tcgaaacctt cggagcagtc gtgccattca accggtgcgt aaagcattgc
     9961 ttcatcagga taaatcatat tgtgtccttt agtcagaaac gcaagatctg cgccagactt
    10021 gcgattacgc cagcgggatt ttcccgatgc gcgttatgtc cggcgcgagg aatgacatgg
    10081 cagtcggcag ccagttccgc cgccagggcg cggaatttgc tgtcacgttc accacataaa
    10141 taataaaacg caaatgtgcg ggcgctaagg ttagcacgta aatcaggctg gacggcgaga
    10201 gaagtcgcct ccagcatggc ggcaagggtt gcgccattat tgttgctgcg cagcgccacc
    10261 agctcccggc gttgatcgtc attgagcgag gcaaaaacag gctgttgata ccagtcggca
    10321 aataccgccg ttaacggttc tgtgcgaaaa cgctgcgccc attggcgatc ggaacgctga
    10381 cgttccgcac gttgttcagc attttgcagc cccggatgcc cgccttcgac aataacccca
    10441 caaagccccg ccagcccctg gcaagccgcc atcatcgcca cccgtccgcc aagcgagtac
    10501 cccaccagcc agaagtcaag gatgttgtaa ctaaccaagg ttttacgcag taagtcggtg
    10561 acatcatcaa atccatcgac gctaatcgcc gccgaaccac catgacctgg gagatcaaca
    10621 tacaaccgtg agtagtcggc aaacgcctcg cccacttctt gccattcgtg gcaatcgccg
    10681 gaaaaaccgt gcaaaaacac cagccagggt aaacctggtt ttccgtgttt tgcctgcgcg
    10741 tgcaggatca taaatggctt acctgcgcca gaagttgctg gagcgtttgc gcaccatcgg
    10801 tgtcgttaac caccatttca atcaccgtgg tggttggcgt gcgccaggcg tcggcaaatg
    10861 ccgtttcaag ttcctgccag ttttgcggac gatgatattt cagctcgaac atcgcggcgg
    10921 cgtgctcaaa atggacgttt tgcggcatca gatagaaacg ctcacgctcg ctttgcggcg
    10981 ttggcaacag cgagaaaatt tgcccgccgt tgttgttcac cacaattaat accagcggcg
    11041 cagaaacctg acgcaataac gccagcgcgt tgagatcgta aagtgcggag agatcgccca
    11101 caatcgccag cgtcggtttg ccgcttgccc gctgaacgcc tgcggcggtc gaaagcagtc
    11161 cgtcgatacc gctggcccct cggttgctgt acaccgggta acctgccgga agttgcgaaa
    11221 gcgcatcaat cagacgtacc accaggctgt taccgacaaa caattgcccc tgttcaggca
    11281 gatagtcgct gatgcgatgc gccagttgcg cttcgccaaa cgcatcgcga cgggcaataa
    11341 ccgcctgcat tgcctgttcc gccaggcgcg ggatttcaac gcaccagggc tggcgttttt
    11401 ctgccggatg cagctccagc cagtcggcaa tattggcaat taagcggcgt ccacgatggt
    11461 gcgccggatc aagccgccct tcgatgtcat ccacaatcca gtactcttcg ggttcacagc
    11521 ttgcctgcca ttgcaggagc cgtttgcccg tcaggctgct tcccagttgc accacaattt
    11581 gcgcctgctg cagctcgctg gtcgctttgg cattgcctaa ccaaagatcg gcacacggca
    11641 gcggctgccc ggtttgtgac agcacatcgc caatcagcgg ccagccaaga gtttgcgccc
    11701 acagggcaac ttttttgccc tcttccgcac tcatgcgccc ggcaaccacc acgccgcgct
    11761 tttgtcgcca gaagaaccag tcgcgctgtt tttcactttc cagactaggc gcttcacgca
    11821 gccacggttt gtcgtcctgc caccagtcac ccagacgctg ttgccagcta agcccggtat
    11881 cgtccatttc gccatacagc ggttcagcaa acgggcagtt gatatggact ccccccgcat
    11941 gaagcgtacc gagagcgtgg tcgatggtag aaaccagcca acgtgcgggg atatcctggg
    12001 tcgggcgcgg caatgaaata ctgtgcgtgg ggtgagaggc gaacattccc ggctggcgaa
    12061 ttgcctgatt cgcgccgcag tcaattagct ccggcgggcg atcggcggtt aagagaatca
    12121 gtttttctcc ggttaacccg gcttcaatca gtgccggata gagatttgcc accgccgtgc
    12181 cggaggtcac aatcaccgcc accggctgct tgctgacttt cgccagcccc agcgccagat
    12241 gccccaaccc acgctcatcg aaatgggtgt ggtgaatgaa tgcggaattc tccgccgccg
    12301 ctaacgttaa cggtgtagaa cgcgagcctg gggcgataca gatgtgtctg acgccgtgac
    12361 gcgttaatgc ttccagaatg accgccgccc agcgtcggtt aaatgcgctt actgacatga
    12421 gtttgtccgg tatcaatatt gcggctaagt ataaggagct acaaaaatag attattgata
    12481 tgaatcggta atgatgcgac tcattactat tccatttgta ataaagtacg caaccctgcc
    12541 gctttgttgt cgatttcctg ccactcttgc tcggggtcgg aaccacggac aatgcccgcg
    12601 ccagcatata atcgcacgac attgccgcta atttttgctg agcgcaggga aacgcagaat
    12661 tcgctttgtt gtaatgagag atagcccgca gaaccggcgt accattctcg ggtgaacggt
    12721 tcgtgacggg cgataaactg tcgcgccaga tcgcgcggta agccagcaac tgctgccgtt
    12781 ggctgcaact gatgtaaaca gatcacatca tccgctttgt tgagtgaagt ccagatacag
    12841 cggcgaagat gctgcacttt acgcagacgc agtacctgcg gcggtaaaac atccagcgtc
    12901 tgggtatcgg cctgtaatcg ttggcagata tcttccacca ccagcatgtt ctcgcgctgg
    12961 tttttatcat ccgccatcag ccactcttct aactgctgcg cctgcttatc atcaggatga
    13021 tttgctactg ttcccgccag cgcttcagta cgcagcgctt tgtcacgccg ccgccataac
    13081 cgttccggtg aagagccaag aaaagcattt tcgccatcaa aggccatgta aaaatggtag
    13141 caattcagat tcagtcgacg actggcagcc atcatcgccg ccgcgttgac cggacttgcg
    13201 aaatgcaggt cagttgcccg agcgagcacc actttgtcga gctcaccttc ggcgatggtt
    13261 ttcgttgcca gttcgattaa ttgcgtccag cccgttttgt ccggccagtg ttgttctcgc
    13321 gtggtggtta aatgtaaccc aggcaagggc ttgatactca ccagtgtggc gataaattct
    13381 tttgcctgaa tcgcatcgtg ctgaagggag ctttcgctga atagcgtcag ccgcagcgtg
    13441 gctttaccgc cacagcgtcg ccattccagg cggggtaaaa gtaaattgcc ctgcgacggg
    13501 tcaaaagcat tcagccccca aatgcgtaag tcggcgtgtt ccgggtgctg gcgaagaaaa
    13561 cgttgtgcct ggtccaacga cgtaaaacgg gtaatcgcgc ccaggacggc agcttcttca
    13621 tcaccattac gttgttgcca gtagaattgc gggtacgttt gctgactggc cagccagctc
    13681 aaggcatcaa aagcgtcttt gagagggaaa ggaatatcga taacccgaat gccgggtgtc
    13741 gccggaatct cttgcgacaa atggcgcagt agattttcca gcgccgtagt aagtgattgc
    13801 acgcggacct ctccctgtta aaagcctcac attatacggg gtactacaaa aaaaatgcag
    13861 taccccggtg tagggaggtt ttaacggcgt gccagcaaca gtcctagtac cagcccaacg
    13921 gccgcgccca caccaattcc ttgccaaggt ttttcgtgga cgtagtcatc agcacgataa
    13981 acagcctgct tcgcccgata gtaataactg tctgaaacct ggctaacccg ttttttcaca
    14041 tcatccagcg ctttttctgc acgcgctttc agctcaacat atttctgatc agcgggatcg
    14101 ccagaggagc ggagcacctc ttccagtgtc tcactaagca gcgtcaggtc gtcatcgata
    14161 cgtgtatcac caaactgatt agacattctc gttctccatt tgcgtaaaac ctgtcactaa
    14221 ctatagacaa gggttgttga ttacgcctga attacctcgc gtgccatccc aatgtgtggg
    14281 ataccatctt cttcatagac ctccgtcaca gggataaaac cgaaactctg gtagaagttt
    14341 tgcagatgcg cctgcgcccc gaggtaaaca ggcttatcag gccagtgatg cgtgcaggtg
    14401 tcgagggttt tactcatcag ttgctgcccc actttttcgc cacgcaacgc ttcactgaca
    14461 atcacccgac caataacgac cggctcaaga tcatcatcac ttttcagaat cctcgcatat
    14521 gccaccagtt catcattttt ccaaccgaga atatggcgat tatcccccgt caaatcatca
    14581 ccatcgatat cctgataagg gcaattctgc tcgacaacaa acaccgcgca acgcagttgt
    14641 aataaggcat aaagctggga aaccgataat tcagagtgat gcagatcttg ccattcaatc
    14701 ataatgctct ccttttagat gtatacctta tactagccgg aatatttttt gaaacgtgat
    14761 gaactcatgg aattaatttt tttaggtact tcagccggtg tgccaacccg cacgcgcaat
    14821 gtcacggcaa tattgcttaa cctgcaacat ccgacccaga gcggactttg gttgtttgac
    14881 tgcggtgaag gcacccagca tcagctactg cataccgcct ttaaccctgg aaaactggac
    14941 aagattttta tcagtcacct tcatggcgat catctttttg gtttacccgg cttgctgtgc
    15001 agtcgttcta tgtcaggcat tatccaaccc ttaacgattt atggtccgca aggtatccgt
    15061 gaatttgtgg aaaccgcgct gcggattagc ggctcatgga ccgattatcc gctggaaatt
    15121 gtcgaaattg gcgctggcga aattctcgat gatggcctgc gcaaagtaac cgcttatccg
    15181 ctggaacacc cactggaatg ttatggctat cgtattgaag aacatgataa accgggtgca
    15241 ttaaatgccc aggcattaaa agctgctggc gtgccgcctg gcccactgtt tcaggaatta
    15301 aaagcgggca aaacaatcac gctggaagat ggaaggcaga ttaacggcgc agattactta
    15361 gctgctccag
//
LOCUS       DAJTYD010000084        15304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTYD010000084 DAJTYD010000000
VERSION     DAJTYD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.084"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            13..363
                     /locus_tag="OO585_002366"
                     /pseudo
     CDS             13..363
                     /locus_tag="OO585_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            577..1725
                     /locus_tag="OO585_002367"
     CDS             577..1725
                     /locus_tag="OO585_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6629930.1"
                     /translation="MFEIDSLLLITSVIILSLLAVKLFDEISPIQLVDHGRNNQIDGM
                     RGFLAIFVLIHHAAIWHGYLLTGVWETPSSNLLTNLGQVGVSFFFMITGYLFFSKIRS
                     SDQDWVRLYISRFLRLTPMFIVSLCLVLLVIGFKSRWSVHVSPASLTVSLMRWAPFTA
                     LGMPNINGVKDSFTINAAVTWTLVYEWFFYFSLPVIAALFKRRVSIHMIIISVLVLVI
                     FLCYPSKKIHVISFLFGLLASYSNNYVHRVAKAKITPIMIIALLSYEMIYFPTTYAPL
                     PLIICGVCFILIASGCDFYGILRLNLTRKLGETTYSIYLLHGVFLYCVMTWVIPSNYS
                     NYFFIMLISITAFCVTLLSCITFKLIELPFINITKQTAMKIRGIIKNT"
     gene            complement(2005..2616)
                     /locus_tag="OO585_002368"
     CDS             complement(2005..2616)
                     /locus_tag="OO585_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6629931.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASINLIQLKLQAG
                     RNLTQAETSRLNTVLDYIDAVTATDTSTAPDVIWPELLDAHNM"
     gene            complement(2616..3398)
                     /locus_tag="OO585_002369"
     CDS             complement(2616..3398)
                     /locus_tag="OO585_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6629932.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTILKQITPLSPSIDF
                     DNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            complement(3402..3986)
                     /locus_tag="OO585_002370"
     CDS             complement(3402..3986)
                     /locus_tag="OO585_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6629933.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMQELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(3977..5035)
                     /locus_tag="OO585_002371"
     CDS             complement(3977..5035)
                     /locus_tag="OO585_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6629934.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(5022..5447)
                     /locus_tag="OO585_002372"
     CDS             complement(5022..5447)
                     /locus_tag="OO585_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6629935.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLRRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(5447..5995)
                     /locus_tag="OO585_002373"
     CDS             complement(5447..5995)
                     /locus_tag="OO585_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6629936.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(5995..7074)
                     /locus_tag="OO585_002374"
     CDS             complement(5995..7074)
                     /locus_tag="OO585_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6629937.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(7071..8399)
                     /locus_tag="OO585_002375"
     CDS             complement(7071..8399)
                     /locus_tag="OO585_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6629938.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(8460..10295)
                     /locus_tag="OO585_002376"
     CDS             complement(8460..10295)
                     /locus_tag="OO585_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6629939.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(10437..10706)
                     /locus_tag="OO585_002377"
     CDS             complement(10437..10706)
                     /locus_tag="OO585_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6629940.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(10706..11062)
                     /locus_tag="OO585_002378"
     CDS             complement(10706..11062)
                     /locus_tag="OO585_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6629941.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(11062..12558)
                     /locus_tag="OO585_002379"
                     /pseudo
     CDS             complement(11062..12558)
                     /locus_tag="OO585_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
     gene            complement(12542..12712)
                     /locus_tag="OO585_002380"
     CDS             complement(12542..12712)
                     /locus_tag="OO585_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6629942.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(12739..13281)
                     /locus_tag="OO585_002381"
     CDS             complement(12739..13281)
                     /locus_tag="OO585_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629943.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHT"
     gene            complement(13278..13784)
                     /locus_tag="OO585_002382"
     CDS             complement(13278..13784)
                     /locus_tag="OO585_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629944.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(13759..14169)
                     /locus_tag="OO585_002383"
     CDS             complement(13759..14169)
                     /locus_tag="OO585_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6629945.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDSLFSR"
     gene            complement(14166..14489)
                     /locus_tag="OO585_002384"
     CDS             complement(14166..14489)
                     /locus_tag="OO585_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6629946.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(14492..14692)
                     /locus_tag="OO585_002385"
     CDS             complement(14492..14692)
                     /locus_tag="OO585_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6629947.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(14741..>15304)
                     /locus_tag="OO585_002386"
     CDS             complement(14741..>15304)
                     /locus_tag="OO585_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6629948.1"
                     /translation="LATEFAEQEEIAFTTGDGTKKPKGFLANESTDETDKVRAFGKLQ
                     HIVSGDATAVTADAIIKLIYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGL
                     ELGQPSSLAGYGIAENEQMPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPF
                     VGFYTTKRTGGMLVDSQAIKLLKIAVA"
BASE COUNT         3502 a         4354 c         3681 g         3767 t
ORIGIN      
        1 gttcactagg ccagtggcag cagcaaaagt tctgatttat ttatcagtct gttagaggca
       61 ttacggcgta gatatcggcg ggcgaaaacc atcacactgg tggcggataa ctacatcatc
      121 cataaaggcc gtaaagtgga gcgatggctg gaagaaaacc cgaagttccg gttgctgttc
      181 ctgccgacgt attcaccgtg gctgaatccg atagagcaac tttggttatc gttacacaag
      241 accataacgc gaaatcatca gtgccggtat atgtggcaga tactgaaaca ggtaacgcag
      301 tttatgaatg ctgctttacc gtttccgggt aatcaacacg gtatggccaa agtggagcgg
      361 taatatgcga agctatttag attcagtttg aatctcagcc ctgacattca ttctttaccg
      421 acgttccatt agcccaaatc tgagtatata acctcaattc aactgactaa atttaacaca
      481 atatcaatga caaatgtctt taccattggt acatacaatt gcaaaatcac catctctaag
      541 caataagact acgcatatta aagtgagtta tattaaatgt ttgaaattga tagcctatta
      601 ttaataacat ccgtgataat cttgtcatta ttggctgtaa aattatttga tgaaatttct
      661 cctatacaac ttgtcgatca tggaagaaat aatcagatag atggaatgag aggattctta
      721 gccatcttcg tacttattca tcatgccgct atttggcatg gctacttatt aaccggggta
      781 tgggaaactc cgtcatcaaa cttattaaca aatttaggcc aagttggtgt ctcattcttt
      841 ttcatgatca ccggatatct atttttttcg aagattcgct caagtgacca ggattgggta
      901 agactatata tatcgagatt tctgcgatta actcctatgt ttattgtaag tttatgttta
      961 gttcttcttg ttataggttt taaatcaaga tggagcgtac atgtctcccc tgcctctctt
     1021 actgtgtcac tcatgagatg ggcaccattc accgcattag ggatgccaaa tattaacggt
     1081 gttaaggact cattcactat caatgcagct gtaacatgga ccctcgttta cgaatggttt
     1141 ttttattttt cattaccggt tatagctgca ctcttcaaaa gaagagtcag catccacatg
     1201 attataataa gtgttttagt tctagtgatt ttcttatgtt acccatcaaa aaaaatacat
     1261 gttatttcat ttttatttgg actgttagcc tcttattcaa ataattatgt acatagagtt
     1321 gctaaagcta aaataacgcc tattatgata attgcattac taagttacga aatgatatat
     1381 tttcctacaa cctacgctcc actccctctc ataatttgcg gtgtttgctt tatacttatc
     1441 gcatcaggct gtgattttta tggtatattg agattaaatt taacaagaaa acttggtgaa
     1501 acaacatata gtatttatct tttacatgga gtattccttt attgtgtaat gacatgggtc
     1561 atccctagta attattctaa ttattttttt attatgttaa taagcattac tgctttttgc
     1621 gtcacacttt tatcatgcat aacatttaaa ttaattgaac tacctttcat taatataaca
     1681 aaacaaaccg ctatgaaaat caggggcata ataaaaaaca cttagtaatc aattgtcacc
     1741 taatcaagag gtggtttata gataacaata aagaaccttc gccatttccg gaggttcttt
     1801 tatatcctta tggtaaatat aaattataaa tattattttt atggtgttcc actatgaaca
     1861 gggaagctaa ataatgatta caaaaatatg taacttcagg caatgcaggg gctatgttat
     1921 tgccttaaca ccataatgag taatattgtg tcccgaggat aaaagtactg ttaattctta
     1981 ttgatgcttc aatagcactc aaaatcacat attatgtgca tccaacaact caggccagat
     2041 gacatccggc gcggtgctgg tatctgttgc cgtcaccgcg tcaatgtaat ccagcaccgt
     2101 gttaagtcgg ctggtttctg cctgcgtcag attccgtccg gcctgtaatt ttagttgaat
     2161 cagattgatg gaagccattg cagtatcaat cagcgactgg cgttgtgctt ctgctgcatc
     2221 gactgcggcg ctatgttgtg cctcggtatc cgtcacccat ttctcaccat cccatttatc
     2281 gtatggtgtt aacgggacga tagtggttgt attatcaggg taatcacccg gagctgtgat
     2341 ttcttttgat tctcctgttt cggtgctaaa gacgatttca ccgcgatggt ctggcacata
     2401 ttcccatgag ttaaaatctg cagaacggca gattgcataa ccagctttgt atgcgcctgg
     2461 agcatctaaa caggaatatg ccggaatgcc gacaccaata gcaagatatt cagttgaagc
     2521 tgaaatatac tcccgactca tgacatcaaa gttataaaca gtaatttctc ctgccatagt
     2581 ggcaattaat tcactgttta attctgcgtt attcattatg cagccctcac aatatagtta
     2641 aaagcgacat ttcgtgggcg tgtttcattc gcacctgcag gtttagtcgt tgccgcaaca
     2701 acaccaccct ctgtaatagc cgggttggga attgggtaat tatcgaagtc aatggacgga
     2761 gacaggggcg tgatttgttt taaaatcgtg gtgcctgtaa aactttccag agtctggtcg
     2821 ttagttcgtg aagtccagat tccccaatag tggttatgac tgacaatagc accgtcctga
     2881 agcgttaata atccccttcc gttatccact ccacgcccgt catcccagcc ccgaataaac
     2941 tcaccgcgta aatcaggtaa tttatttgtc ggataagcct ttgccagttc cgggtattct
     3001 tcagcagaaa aagctgctcc gttgcatttc agccagcctg ttggcggagt ggctgaaggc
     3061 cacggaacag gcacaccaac gggtaatgct gagccttctc ccaaaccaag gttttcgaga
     3121 gctgttttca ccgtgccatc cgatttgata tcgccaaacg gattcttgcg gcttaacagc
     3181 agcgcacgaa gcgcggtaag cagctggtca tgccgcccct tctccaggct ggcaccggat
     3241 gcctccacaa cgctgcaaag ctcctcctgc aacatgtcaa agtagtcatc atccagatcg
     3301 gtggcaggcg tgccggtctg ggggttacca cgggtaaaac cgttcttacc cgcgccgaac
     3361 ttatccttct gcgcggtttt cgtgtctata cgatgcatgg attactccgg atatttaaaa
     3421 atcacatagg tatgcgacgg gcagagtttg ttaagaacac actcgacaac ggtgtccccc
     3481 cagaagcgca gcgcggaatc acagggatcg ccacatgtca tccaggtggt gttggtggcg
     3541 gctggcatgt tgacctgcca gtaataccgc cattccggcg cattcaccgc gtcagtacag
     3601 gcagatgagc aggtgaacgt gcttttgtcg tatcgcgtga tggtggcgtc tggtctgccc
     3661 aaggcagcaa gctgtgcaag gtaaaaatcc tcattgatgc cgcccgccag gttaaccttc
     3721 gcatccagcc gttgctgacg ctggcgaagg gtctgcgtcc ctgccggaat acattcatcc
     3781 ggcaggccgc acagacgctc ccagcggttt atcagttcgg tggtggtgcg cggatccagc
     3841 tcctgcatca gggcatccgc acgctgatga acacgggtta atgacggtgc cgcaccggca
     3901 atcgccggat cgctggctga ccacgccgga ccgggcggca acagtgctga caacagacgg
     3961 atgtaatcat cgtttgtcac gtccatgaaa tcgcccccag aaccgccagt tcatttttcg
     4021 caatggtgat attgtccgcc ggtgcaagca actgatggct gtattccccg ttcgcaccgg
     4081 aaatcgcttc actgatacgt gacaccttca gttctccctg cggataacca tcacgcagca
     4141 ggaacgaacg caactccgcg gtgatggcag cccgtatttc cggtgtgtcc ggcgtcacac
     4201 ggatatgaaa atccaccgta tgtgccaccg gcctgaacac atacaaatca gagcctgcca
     4261 ccggggccag tggcccgata tgttgtcttg ccgccgtttc cgttgattct tccggaatgg
     4321 gattaatcag gtcactgctg gcaatcatca caccgacagt ccccgttccc atccagtgtc
     4381 ggtatgtcca tgcgcgggta atgccaggca cttctttagc ccagacgaca tagtccccgt
     4441 cagccccgcc ctgcggcgtc cagtaatacc gctcaatgac gcgggcgcgc cacgtttcca
     4501 gctcttcagt atcaaatccg cctgtcaggg tgtcagccac accggaagac ggcagaccat
     4561 tcaccggcgt gaccaggatt aatgccgtac cgtcgtcagc gttaccgacc gcacctgcac
     4621 ttgagcaggc gatcggcacg cgcaggacac caccggagct gattgcatcg tcagttgtcg
     4681 tgtactgcac caggtcatcg cgctgaataa cactcccggc agtcaccttc aggccatcgc
     4741 tgacaccttc ccagcgcata tacccgctgg caaccgtggc ccccttgcgc ggacaccgtt
     4801 tcatcgcagc atgtcgcgcc agccaggact catcgcacag gtcaggcagc atattcattg
     4861 ccagataatc gatgtaaccg tagaccgtat gcagcgccgc cgcatacacc tttgcccgca
     4921 cgtcttcatc catgcgccgg agcgtgtcgc tgacgtccag cctggcgaat aaatcgttac
     4981 ggagcatact gatattttct gccagcgtcg ggcgctgaaa ttcactgtcc gccatgcgtt
     5041 atcgcactcc acagatcatc aaaagaaatc attactggtc catcacgacg cctgagggtg
     5101 atactgttac ccagctcatt aatcccggtg cggcggatat ccagatcaat acgggacacc
     5161 acgccgtcat cagtcatcca ttgcaggcat tcgcggatat atccccttac cgtctgcacc
     5221 agctgattgg tcagtttgct gcgctgaagc aaccacagtc gggagccgta acggtcattc
     5281 tgtaccgcag gccaggtatc cccccaccat cccatcggga cgtcggcatt gtcatcaggc
     5341 tccgcccgcc gccaggtgaa cagggaaatc accacggcgc gggtcagcgg atccagcggt
     5401 gcgctggcgc aggtgcgttt accgttcacc gtcagccaca gttccatcat gcctccatcg
     5461 ctttatcagg tttgtcggtg ttactgccct gaccgttctc tctgtgacga tgcccgttat
     5521 aggcaagccg catcgctgac atggtggtgc cgccggagtc gcacaggtct ttcacctgtc
     5581 cggtcacttc cagatccatt tcaaaacgtg cttcaggtgc attgcgaaac gtgatcgttt
     5641 tccctgcacc gtccaccacg atcccctccc gggtcagcgt cacagactgc ccctgatcgt
     5701 catagacagc cacctcaccc gtttgcagcc ctttcaggcg gtagcgccgg tccgacaccg
     5761 taacaaccac cgcatgagaa cggtcgccat ccggaaacaa caccaccgct tccgcaccgc
     5821 tgtttgccct tgcggtaaaa ccgtagggtt caagatgttc aaccccggct ttgggttcac
     5881 cggcaatcag ggacacatcc acggtctgac atttcgtggc ggcactgatg cttttcacca
     5941 ctgcccgccc aatcaggccg agaagttgtc gctgcatggc ttcaatcgtc ctcatcagaa
     6001 cgggtcctcc tgtactctgg cttttttctt tttccgcgcg ccgggggctt cgggttcagg
     6061 cagataagcg tcaggcgggc cgacacggat ttccgtcagg gtgccgttct ggtcctgagt
     6121 aaacgtgact tccgagacaa gcagttcggt attgtcgaaa ccacagaccg gatcgaagac
     6181 aatcacccgc tggttgggct gccacagcgt accgttaccc tgtcgccagc cctgcaccac
     6241 ataggtggtt tcatccgtcc gcgccgcccg ttttcgggct tcaaagtcag cacgcgcaat
     6301 acagcctgcc cccgtggcct gccctgtctg cctgatatac atcggacggt aacgggcaat
     6361 aaatgcgtcc tctgtgcggg cccgcagcgc ggtggtggtg gcctcaccga aatcatcgtc
     6421 gtttccggca cgctgccccg ccacctggta aacagaaaac cgctcccgga tactcttctc
     6481 cgtatcacag gaaaggatgt tttccccaag taccagcgcg gtatgtgccc gcgttgagcc
     6541 aataccgcca atcaccagcc tgccgtgcgg gtcgtcgtaa gccagcgcct gctgctgacc
     6601 gagtattttg ttgattacct caatcaccgt ttcaccgtga tcaggctgaa catcaggaat
     6661 aacacccgac ggcgcaccgc tgttcaccac ctcaatgccg aaaggcgcag caagcgcctg
     6721 cgcaatctgc accagcgagc gtccgttaaa ctgtgtcggt tcggctgcac agtcaatcag
     6781 gtcagccgtc agactacgtc cggcaatacc ggtgctgacc gaacgggcat cgtaacgaac
     6841 gggagtcgcc tccacccagc cggtgatcac cagctcatca ccaatcagca cttccacttt
     6901 tgaaccgttt ttaatgcgcg gctgaagcgt ggtgataccc tcatctcccg gccattggcg
     6961 agtgatctcc acactgaaat cccgcgccag ccgttcaata ccggcaccga tgcgcaccga
     7021 tgtccagcca ttccactccc ggccatttac ccgtagcgtg acattgtcgt tcattgcact
     7081 ggcaccttca gagggatcac cggcacaaag ccgggatgcg taatggcatt acgccgaata
     7141 atgtccgcgt cacgcgccgc gttatcaaac caggtcgccg ccagcaccag cgcgggtaaa
     7201 acctcatccg gcgtgcgctg aatgatccgt gcagactgtt caaggcgcgt gttgatatcc
     7261 gcattcagat ctgctttcac ccggcgcagc gccagaaaca gcgcatcact ggttgtacgg
     7321 gacaactcct tatcaattgc cgtattcagt gtgtcgcgaa tgtcggtcag ttcttcccac
     7381 gttggcaggt caaccgtgtt tttcaccgcc ggtgcattgt tcagtgccgg atgcgtgaca
     7441 gaaggccagc cggtgctctg cgcgggtgtt gttgactgcc ctactgtggc attctgcatc
     7501 accgcggaag ttgtgggcgc aggcaatcgt gtgacggcat acgccgcttc gctgattgcg
     7561 gtcgtacgaa gggtgctggc aaccacgtta cgctgctgcg tcgccgtggc ggtagtttta
     7621 ctgtccgttt tccagacgcc gcgcggttgc agatcgctgc cgaggctgac accggaaagc
     7681 gttttgatca tggtgaccag gtcgctggcg ttaccataaa ggcgtttccc ggtacgccac
     7741 attttctgca cctgctcaac gaaatttttg cctgacgatg gtggcggcag aagtaccgag
     7801 atatccccct gcaacagcct ggcggcatcc gatacggcag aatccaccac tttcatcgca
     7861 tcagaaacat acccaagcat tgtgctggca ttaccgacga cgtcgttctg cacaaaatct
     7921 gccacgccat cgatactgaa accactgaag ctgtcactga tgcagtcatc cagtgcagaa
     7981 caggatgaca tcagcgtctg cgccgtcgcc gcacctgaag tggggtaaga gagttctccc
     8041 gcttcgacaa acttcaggtc aaagcggaca atacgccctt cactcttcga tgtgctgacc
     8101 cgaacttccc cgtcaacaca gactttcagc tcaccgtaag tcggatggac aagcgtgccg
     8161 ggaccgggtt tattcagcgc gtcaatcagg cgatcgcgct ggtcaaagca gtcatctccc
     8221 accacataag ctgtgatgga cgggcggaaa gtgattttcc ccaggtcttc ggtatagggt
     8281 ttgtcgcggt tcgggtattc gtgcgtttcc acacgacgac cggttcccgc actttcttct
     8341 tcaaccttaa acggcacacc gcgaaatgac gcgtcctgaa gtctgtcttt ccacgtcata
     8401 taaactccgt acataaaaaa tcccaccgga gtgggactca ttaacagatt aatttttcat
     8461 tacctgccaa agcgcgtata gccaacatca tggctgacat caaaaccgct ggatcgcgtt
     8521 tccataaccc gcatacccgg aggcgaattc acaaaagaga ccttgatctc accatcaact
     8581 tttggcgcag aagctttgtt aatcatgaag ggattcgggc ctgtggcacc ggaggcgttg
     8641 tttgactgag ccggatctac cgcctgataa ggtgtgtatc cccgtgccgg tattcccgtc
     8701 ccataagcat cataagcacc cgcgccccac tgcgcagagt taatggcatc gaccgtgtca
     8761 ccggaactgt cggtaaacca ctcaataatt ggcttcagtt tgtcccacat atcctgaaac
     8821 cacttaacaa ccggtcccca gttattgatt accatcccca gcggcgacca ggcaaaaacc
     8881 ttcttcagaa gttcccagcc agcctcaaaa taaggaccaa tggtttccca gagcttcttg
     8941 aaataaggtc cgacaacatc ccagttagtg ataattaatc ccgcagccag ggctatcgcc
     9001 gtcgcaatca ttccaatcgg cgtcatcgac atgatcctgc tgacaatact gatggcaccg
     9061 ccaacgccca tcaatcccag tttcagaatc gcaagaccgg cagcaagccc gacgacgccg
     9121 cgaataaccc ggggattttc atccgcaaac ttcgtgaatt tttcccctaa ctcccccagc
     9181 cattgcgtga tatttttggc gtcaccagaa aatgcgccgc caatagccgc aaggccgtta
     9241 gttgcggtcc ccgtcattgc ctcccacagg ttggacagcg taccaagctg ggcctgaaca
     9301 cgtttattca ggctggcctg tttattcatc ttctgctgga tctgatcgta gccatccttt
     9361 cctttatcga ttagtgcatt gaccacctga agggtttcgg catcatcacc aaatattgcc
     9421 ttaagtacac ctgttcgctt aacgtcggtc agttttcgca gctttgccag ttgcctgaac
     9481 atgttatcaa gaccgccaaa acttcctttg ccgtcagtaa aatcgagctg taccccgagt
     9541 ttctggcggg ccatgatttt attgacgtcc ctgattttct taacgcttaa tccggactgg
     9601 ataacttttc gcagggcgtt acctgccgac tccccgttca tccccatctg atccatcatg
     9661 acgctgatgg gggcaaggct ctgtgcagcc tgaagaccgt ccttgttcac catcttcaga
     9721 acagagctgg ttttagtgaa gaaggacaac atgttggtat cgtcaacacc cagataaaac
     9781 gccttctgga tagtgtcgaa cagccccatc atgtcttctg acgccgttcc ggtagcatcc
     9841 tgcatctttg cagcaaactc agcagccgct tccggtgttt ttttcagttg taccgcaaga
     9901 taagctgtcg ctttacccac accgccaaga atgttttctg ccgggatccc ctgacgcacc
     9961 agcatctgca tcatgttctg gaaatcagcc gttgtaccgg gtagctggtt acccaggcca
    10021 atagccagtt tattgatgtc ctgaaagctc tttccgacct cgccgttcgc atccatcatg
    10081 gcgactttca gcccggtggc ggcgttttcc tgatcggcat aagatttcag ggaaagcgtc
    10141 agacccgctg ccagtccgcc accaagcgcc agcccaccct gtgacgcttc ttccgcctgg
    10201 cgtttaaatc cccggatttt cttttgcatt ttcgacagcg cgggagaaag cctgtcgaca
    10261 ccggtgatca acgccttaag ctcaaattcc gccatgtgtg cgtttctcct gctctatcct
    10321 gtttgcctga ctgaccagca aggtaatttc actgatcggc atattcagca attcgaaagg
    10381 attaatgcgc cagtaactgg cgcagtcaaa gaagcgatca gtgaggtatt cagccgtcag
    10441 gcctggagga aaaaaccagc cacaagccac gccgctgcat tcaggtctgc cggagacatc
    10501 tggtcgacag agctttgcgg cactttcgcc agccgcacaa tgtatttcga caccacatgc
    10561 gccagaagtc tgactgactc atcctgattc atctggtagg gataccccag ctcgcggaca
    10621 tccttcccgg tgggttcatc aaactccagt acggagagtg tctcgccatg agcagtaatc
    10681 ggtttcttta actcaagctc tttcattact ggtaatcccc ttcttcaccg tggaactcaa
    10741 gatcaaccgt gccttcttcg gcattatggt tcgcttcgcc gtgcagccag gcagacgaca
    10801 gtacatagac ctgaccgttc gccagctcgg cagtgatggt catctcatca gacgaggtga
    10861 ttttgcttac cggaaaattc ttcggcacct tgaaggtccc tttgacataa ggcgcacggt
    10921 gagtttcctt gcggtccact gaaccgtcca ggccgatgat gtcatcgtta accgttttgt
    10981 tcatgggcac ctcaatgccg ccggtcagcg atagctgctg accgtcaatt ttgaaataac
    11041 aggttccccc gatacgggcc attatgcaga ctcctctgaa tactgaagac ggaactgatt
    11101 aaccacggca aagacacgca gctggttaac atagtcaggc gggaacagcg tgttcaggcg
    11161 gttcggatcg ctggcatcac gctccacaac caggtactgc ttaaacagtt cgtagttttc
    11221 cacgatcccc gcacgctcaa gctgacggta ggttgccagc agttcccctt tgatcaccgc
    11281 cggggtgaca atcgcctgac cgggaccaaa gcgggtaccg tcactggcaa gcttgtgacg
    11341 cccgtactta ctggtaatga cggatttcag tttgcgcagt acatacgcgc tggtatgcag
    11401 cgtctcactg tcgaggtaac tgttatccgc aaccccgtaa gcgtttttcc tgtacgtggt
    11461 gacatcacgc tgaatgcgta gtaccccgct ttcgacatac gccgttgcca cgccatgaga
    11521 cagcagggtc tgttgttcgg tcatcgtgaa ccgtttcccc ttcggcgcag gcagcatacc
    11581 caccagctca ccggtctgcg tgggacgtgc cggatcgttg cggataaaca ccgctgcgcg
    11641 ggcggtacgg cttgccgcca gctcgtcggc aggcgtctgg gtctcttttt cgtaccccgc
    11701 cagggtaatg tgctgctggt taaactggtc acctgcggtc accagttctg acagcgtgcc
    11761 gatctttgcc gtatacacat gaccatacag ctgacgcgca tagctccagc gaccgctggt
    11821 atcgttcatc tcggtcacca gcgtgttaac ggaggccgtg tcgttgaacg gcaggccgat
    11881 ataatcaaac ggctcatccg ccattgcagc caccgcgccg gtgagaaccg gagcacccgt
    11941 tccggcggta cccgtcgcca cggcaatctg tacgcccgct ggcagcactt cgcccccacc
    12001 aaagccgtag taattgaggc tgacaggaat ttcattccca caaagcccct tatgacgcgc
    12061 ggtcagtgtg accacgcctg ccgaagatga agccgtaaac ggcagggtcg gaacggcatt
    12121 gatggcatcc tagatactgc tggcaatcat cgtgacgtta tcgccgttag tcaccggtgc
    12181 ctgcacgcgg gtacgtccta catacacatt caccgtgccg gtttcggttg ccgccccggt
    12241 caccgtcagc gtaactgttg ccgccgcacc tgtggattca ggaacggcaa tcacatacag
    12301 ctcgccaaac gggtcagtct ggcgataagc ctcgaccata cgcgccagct gacttcccgc
    12361 accacaaatc tggcgtgcat agtctgccga cgacatcagt accagactgt tggcaacaat
    12421 ctctgcaccg ttattggcat gaccaatcag cagcgatgct ccgctgtcct gtgcagtatt
    12481 cgccgcctgg ttatccattt ccgcataaaa caacggaacc agcgtattcg acggaatggt
    12541 gttaaagctt atcgtcatcg gtgttcacct ttttattcac gcgccggata tcacccgctg
    12601 cttcacggcg cagccagtag ttgttctcgt caacatttcg cccttcggcg ggcaaaaggt
    12661 cgccgcgggc agggtcaggc actgaccgcc ctttaacagg tttgacaaac atgaggatcc
    12721 tcaggaagga agggttattt aggtgtgatg ttcgatatcg ccgtcaggcc cgttaccggg
    12781 ctcgagataa tcaacatcaa tcgccagcgt ttgcagttca tccagactgt tcagatcatc
    12841 ctgctggcgg gtatcgtctt cagtcagctc gctgatgacc gaaaaatcga actgataaat
    12901 cagctcatga cgattcagat ccagcagcgt gccgccgtca taggtaatcg ggttaccgca
    12961 cgcctccggg ttccagccca gcagagcctt aaagagcatc tgccggacat cgtccaccac
    13021 atcatacgag gcaaactgac cacgctcatc acgcccatta ctcagtatga caaccacgga
    13081 gaaaccctct ttcagctcct gccagtagtc ggtctggctt ttgttttctc ccggagaatc
    13141 atcacccggt acaacatatg ccgccgggag tttcagcttt ccgacctccg gcagattttt
    13201 gaactgggcc gcgcctgcaa cccggttttc aaaatacgga caacgggcac gcagtgcagc
    13261 aataacagga gtcagtttca tctgtgtcgt cgctccggct tcagtgattt acgcaattcc
    13321 cgcgccagaa aatagcgtgt ccagctgcgg ttcttttcaa gcgtttccac catgaagtta
    13381 ttacgtggag ccagtcgcca gccgctgcca ccggatgcac cacgatgatg actacgacga
    13441 cgttttgctc ctccccggac accaaaaaac agaaacgccg gatagaagtc accagagatc
    13501 atccggttcc ccttcccgtt gcgctggtta ggggcaatgc gtgtcataaa accggctcgc
    13561 tttttactgg ctctcggcac catataacca atcgaacgag ccaggcgtcc ggtctgataa
    13621 ccggggtttt cacccggtgc cgaccgcgca cggcgcatca ccagccgacg ggcatcacgc
    13681 atatgacgct gcccaatcgt gacaaacgcc cgccggacac gagcgcggtt aaagcgcatc
    13741 tcggcgggct gctgaacatc aacgtgaaaa aagggagtcg ccattgctgc ctccgtgact
    13801 ctgcctacat tcgcccagct ccgtacactc cagcagcaga aagcgccgcg ccccgttcag
    13861 atcacgttga cgtttcaccc ggtacacact gtcatcacag accacctcat aatcagcagt
    13921 gatcccccgg cggtaacgaa tggtgatgta atgggtgatg gcgtccccgg tctgcgcggt
    13981 ttcctgccag gtggtggcac tggtctggat aaccttcgcc catgtccgga acgtaaccgg
    14041 gtattgaggc tccacgccaa agttatccgc gggcatatcc acccgctggc ggatcaggac
    14101 gcgtttattc agttcaccgg ggtccggcag aatgtaggtt gcgctggtct gcgcctgacg
    14161 aattttcatt gcggaaagta cctgtacggg ccgacaagcc agccaaaact ctgcggcatg
    14221 tcgagtttct ccacttccgt aaccgacgag cggttttcgt aaaaatggct gataagcatc
    14281 agcatcccca gacgaatatc atccggcagg tgcagcccgt ccggatcgct gtccggaatg
    14341 gtttcatccg gtgcatagag cttccggttc agatacgttt ccgtccgctt ttgtgccgca
    14401 caggccagca gttgcagatg gcggtcatca gcatcgaaat cctcatccag ccggagttgg
    14461 gctttaatct cttccattgt cagaagcata ctcagccctc tttactggtc gtggcttttt
    14521 tctcttttgt cgctttactg ctttttgcac tggttccgcg ctctgctaac ccggcctgaa
    14581 gtgcaatctc ctgcacccgg gcaggaagcg ccccgtcgtc atactcaccg gcccgaatga
    14641 cctcaacacg cataccgtcc ggtgaccatt tcagatcttg tttcaggatc atgattcttc
    14701 acccgtcaga acaggggcgc ggttccgcgc ccctgagtga ttacgccact gcaatcttca
    14761 gcagtttgat ggcctgcgaa tcgaccagca tgccgccggt gcgtttggtg gtataaaaac
    14821 cgacaaacgg tttattggtg tacgggtcac gcagaatgcg ggtgccgata cggtcaacga
    14881 tggtgtaacc ccgtttgaag ttaccaaatg caatggcttt cgcatcagcg gcgatatccg
    14941 gcatctgttc gttttcagcg ataccgtaac ccgccagaga ggacggctgc cccagctcca
    15001 gccccggacg ccacagatag ttaccctcgc tgtctttcag cagacggatg gcaaacaggc
    15061 tattgttgtt catcatgaac ttcgcgccgg tgcggtgtgc cttacgcagc gtgtaaatca
    15121 gtttgataat ggcgtctgcg gtcaccgccg tcgcgtcgcc ggatacaata tgctgaagtt
    15181 tgccgaacgc ccggacctta tcggtttcat ccgtggattc attcgccagg aaccctttcg
    15241 gcttcttggt accatcgccg gtggtaaagg caatttcttc ctgttcggca aattcggttg
    15301 ccag
//
LOCUS       DAJTYD010000085        15226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTYD010000085 DAJTYD010000000
VERSION     DAJTYD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.085"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1741
                     /gene="crfC"
                     /locus_tag="OO585_002387"
     CDS             <1..1741
                     /gene="crfC"
                     /locus_tag="OO585_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6629949.1"
                     /translation="IENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYA
                     AIEHIPVIEVEFVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAV
                     LDYTQLKSISDEEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKG
                     CITPQQIFPVSSMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLA
                     DLEHIRHAADQLWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDF
                     RAHGLNVACEQLRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDA
                     LNAQLAALFQDDSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIR
                     PIEQQVKMELSESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVR
                     ETFSRWLNQPDWGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTA
                     GMNTFFAEFASCLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLL
                     RDDIQTLFTAERY"
     gene            1738..2621
                     /locus_tag="OO585_002388"
                     /pseudo
     CDS             1738..2621
                     /locus_tag="OO585_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            2885..4387
                     /gene="proP"
                     /locus_tag="OO585_002389"
     CDS             2885..4387
                     /gene="proP"
                     /locus_tag="OO585_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6629950.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            4453..4590
                     /gene="pmrR"
                     /locus_tag="OO585_002390"
     CDS             4453..4590
                     /gene="pmrR"
                     /locus_tag="OO585_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6629951.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(4566..5666)
                     /gene="pmrB"
                     /locus_tag="OO585_002391"
     CDS             complement(4566..5666)
                     /gene="pmrB"
                     /locus_tag="OO585_002391"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6629952.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5667..6335)
                     /gene="basR"
                     /locus_tag="OO585_002392"
     CDS             complement(5667..6335)
                     /gene="basR"
                     /locus_tag="OO585_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6629953.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6332..7975)
                     /gene="eptA"
                     /locus_tag="OO585_002393"
     CDS             complement(6332..7975)
                     /gene="eptA"
                     /locus_tag="OO585_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6629954.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8079..9416)
                     /gene="adiC"
                     /locus_tag="OO585_002394"
     CDS             complement(8079..9416)
                     /gene="adiC"
                     /locus_tag="OO585_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6629955.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(9553..10314)
                     /gene="adiY"
                     /locus_tag="OO585_002395"
     CDS             complement(9553..10314)
                     /gene="adiY"
                     /locus_tag="OO585_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6629956.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(10639..12906)
                     /gene="adiA"
                     /locus_tag="OO585_002396"
     CDS             complement(10639..12906)
                     /gene="adiA"
                     /locus_tag="OO585_002396"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6629957.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13105..14013)
                     /gene="melR"
                     /locus_tag="OO585_002397"
     CDS             complement(13105..14013)
                     /gene="melR"
                     /locus_tag="OO585_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6629958.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14296..>15226
                     /gene="melA"
                     /locus_tag="OO585_002398"
     CDS             14296..>15226
                     /gene="melA"
                     /locus_tag="OO585_002398"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6629959.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKEL"
BASE COUNT         3645 a         3696 c         4027 g         3858 t
ORIGIN      
        1 gatcgaaaat ggcgtcgctt tcgaaaaata ttatctgggt gcccagccta tttttcattg
       61 tctgaaaagt ttgaatgatt tagtgcgact ggcgaaggcg ctggacgtcg attttccttt
      121 ttctgcttac gccgccattg agcatattcc cgtgattgaa gtggagtttg tccatctggc
      181 ggggctggaa agttatcccg gtcagttgac gttactggat acccccgggc caaatgaagc
      241 cgggcaaccg catctgcaaa aaatgcttaa ccagcagctg gcacgcgcct cggcggtact
      301 ggcggtgctg gattatacgc aactgaaatc gatctccgat gaagaggtcc gtgaggcgat
      361 tttggcggtg gggcaatcgg tgccgctgta tgtgctggtc aataagttcg atcaacagga
      421 tcgtaacagt gacgacgccg accaggtgcg ggcactgatt tccgggacgc tgatgaaagg
      481 ctgtattacg ccacagcaga tatttccggt gtcgtcgatg tggggctacc tggcgaatcg
      541 ggcgcgctat gagttagcca acaacggtaa gttaccagcg ccagagcaac aacgctgggt
      601 ggaagatttt gcccatgccg cgctcggcag gcgctggcgt catgccgatc tggcggacct
      661 cgaacatatt cgtcatgctg ccgatcagtt gtgggaagat tcgctgttcg cccagccaat
      721 tcaggcgttg cttcatgccg cttacgctaa cgcctcattg tatgctctgc gatctgctgc
      781 gcataaactg ttgaattacg cgcagcaggc gcgggaatac ctggattttc gtgcgcacgg
      841 gttaaacgtc gcttgtgaac aattgcggca aaatatccat caggtcgaag aaagtttgca
      901 gctattgcaa ctcaatcagg cgcaggtgag cggcgagatt aaacatgaaa tcgagctggc
      961 cctgacctcc gccaaccact ttctgcgtca acagcaagat gcgctgaatg cgcagttagc
     1021 cgccttgttt caggatgatt cggagccgtt aagcgagatt cgtacctgct gtgagacact
     1081 gttacagacg gcgcagaaca ccatcagtcg cgactttacg ctgcgttttg ccgagcttga
     1141 atccaccctt tgccgggtgt taaccgatgt tattcggccc attgagcaac aagtcaaaat
     1201 ggaattgagc gagtcagggt ttcgtcctgg gtttcatttt cctgtttttc acggagtagt
     1261 tccccacgtc aacactcgcc agctgttcag tgaagtcatt tcgcgccagg aggcaacgga
     1321 cgagcagagc acgcgtttag gcgttgtgcg tgagactttt tcgcgctggt tgaatcagcc
     1381 cgactgggga cggggaaatg agaaatcccc gacagaaacg gttgattaca gtgtgttgca
     1441 acgagcatta agcgcagaag tcgatcttta ttgccaacaa atggctaaag ttctggcaga
     1501 gcaggtcgat gaatctgtta cggcaggcat gaatactttt ttcgctgagt tcgcttcatg
     1561 tttgacggaa ttacagacgc gtttacgcga aagcctggct ctgcgtcaac aaaatgaatc
     1621 ggtggtcagg ctgatgcagc agcaattgca gcagactgtg atgactcacg gctggattta
     1681 caccgacgcc cagctgttac gcgatgatat tcaaacactt ttcacggcag aacgatattg
     1741 accaagacgt tactggacgg ccccggtcgc gtgctggagt cggtttatcc ccgcttttta
     1801 gtggatctgg cgcagggcga tgatgcccgc cttccacaag cccatcagca gcagtttcgt
     1861 gaacgactga tgcaggaact tctttcgcgt gtgcagcttc agacatggac gaacggcggc
     1921 atgttaaatg cgccgcttag cctgcgactg acaatggtgg aaaaactggc gtcgatgctg
     1981 gatcccggtc atctggcgct gacgcagatc gcgcagcatc tggcgctgct gcaaaaaatg
     2041 gatcaccgcc agcactctgc tttcccggag ctcccccagc aaattgccgc cttgtatgag
     2101 tggttttcag cccgttgtcg ctggaaggaa aaggcgttaa cgcaacgagg ccaggcctac
     2161 tggtgcaggc aggtgatcag agcgagcaaa tttttacccg ctggcgtgct ggggcgtata
     2221 acgcctggtc gttgcctggg cgctgtttta tcgttctcga agagttgcgt tggggggcat
     2281 ttggcgatgc ctgtcgtctg ggaagcccgc aagcggtggc gttgctgctg ggtgatttgc
     2341 gcgtgaaagc gacacaacat ctggcagaga gtatcaatgc ggccccgacc acgcgtcact
     2401 attaccatca gtggtttgcc tcttcgaccg ttctgacggg cggggatcat gctgattttt
     2461 taagttggct gggaaagtgg accacggcag ataaacaacc cgtttgctgg tcagtgaccc
     2521 aacgttggca aactgtcgcg ctggggatgc cacgactctg ttcagcgcag cgtttggtgg
     2581 gggcaatggt cgaggaaatc ttctctgtaa atttggcgta aataatcagt tacatcaatg
     2641 agtcctaaac gaaatccatg tgtgaagttg atcacaaatt taaatactgg tagggtaaaa
     2701 aggtcattaa ctgcccaatt caggcgtcaa ctggtttgat tgtacattcc ttaaccggag
     2761 ggtgtaagca aacccgctac gcttgttaca gagattgcat cctgcaattc ccgctcccct
     2821 tttgcggccg tcgcgctgat ttttctggcg tttgcggaaa tgggccaact ctgcgaggaa
     2881 agctatgctg aaaaggaaaa aagtaaaacc gattaccctt cgtgatgtca ccattattaa
     2941 tgacagtaaa ctgcgtaaag ccattaccgc agcatcactg ggtaatgcaa tggaatggtt
     3001 cgattttggt gtttatggtt ttgttgctta cgcattaggt aaagtttttt tcccgggggc
     3061 tgaccccagc gtgcagatgg ttgctgcact tgccactttc tccgttccct ttctgattcg
     3121 accgcttggc ggactcttct ttggtatgtt gggcgataaa tatggtcgcc agaagatcct
     3181 cgctatcact attgtgatta tgtcgatcag tacgttctgt attggcttaa taccgtccta
     3241 cgacacgatt ggtatttggg caccgattct gctgttgatc tgtaagatgg cacaaggttt
     3301 ctcggtcggc ggtgaatata ccggggcgtc gatatttgtt gcggaatact cccctgaccg
     3361 taaacgtggc tttatgggca gctggctgga cttcggttct attgccgggt ttgtgctggg
     3421 tgcgggcgtg gtggtgttaa tttcgaccat tgtcggcgaa gcgaacttcc tcgactgggg
     3481 ctggcgtatt ccgttcttta ttgctctgcc gttagggatt atcgggcttt acctgcgcca
     3541 tgcgctggaa gaaactccgg cgttccagca gcatgttgat aaactggaac agggcgaccg
     3601 cgaaggtttg caggatggcc cgaaagtctc gtttaaagag attgccacta aatactggcg
     3661 cagcctgttg acatgtattg gtctggtaat tgccaccaac gtgacttact acatgttgct
     3721 gacctatatg ccgagttatt tgtcgcataa cctgcattac tccgaagacc acggggtgct
     3781 gattattatc gccattatga tcggtatgct gtttgtccag ccggtgatgg gcttgctgag
     3841 tgaccgtttt ggccgtcgtc cgtttgtgct acttggtagt gttgccctgt ttgtgttggc
     3901 gatcccggcg tttattctga ttaacagtaa cgtcatcggc ctgatttttg ccgggttact
     3961 gatgctggcg gtgatcctta actgctttac gggcgttatg gcttctacct tgccagcgat
     4021 gttcccgacg catatccgtt acagcgcgct ggcggcggca tttaatattt cggtgctggt
     4081 tgccggtctg acgccaacac tggcggcctg gctggtcgaa agctcgcaga atctgatgat
     4141 gcctgcctat tacctgatgg tagtggcggt gattggttta atcaccggcg taaccatgaa
     4201 agagacggca aatcgtccgt tgaaaggtgc gacaccggcg gcgtcagata tacaggaagc
     4261 gaaggaaatt ctcgtcgagc attacgataa tatcgagcag aaaatcgata atattgacca
     4321 cgagattgcc gatttgcagg cgaaacgtac ccgcctggtg cagcaacatc cgcgaattga
     4381 tgaataagct gaaacggatg gcctgatgtg acgctgtctt atcaggccaa ttgaactctt
     4441 aaggttcact taatctctga cgcgcatact ctcctccagg ttaacggagg agagtgcaat
     4501 gaaaaaccgt gtttatatga aagtttaact accgtgttca gcgtgctggt ggtcagcagc
     4561 tttctttata tctggtttgc cacgttctga tctttcttca gccgtaccca ggcccgcgtg
     4621 ccggaagtct cttgccggtt ttgcaggaaa aactgcccgt gatgcaactg tgtaatgcgg
     4681 ctgacaatac ttaaccccag accaatcccg ccataacggc tgtccatacg tacaaacgct
     4741 ttactcaact ccccgcattt actctcatca atacctggtc cttcatcttc aactgccatg
     4801 accgctccgc catcttcttg cagcttaatc ataatgttgc tgccttgcgg gctgtaacga
     4861 tgggcgtttt ctaccaggtt tcgcaataac atccgcagca gggttgcatc accctgaacg
     4921 gtgatgtcgg cggcgctctc tggcaatagc agggtttgct gtcgctggtc gagcatggta
     4981 ctgagttcgt catacgaggg gagaatgaca tcttccagca gttttacatg ttgataatta
     5041 ccggaagaaa atgactgtcc ggcacgcgcc agttgcagca gctgggagac gctctccatc
     5101 atctgatcaa gccgtgccac taacggtgct acatcaatgt gatgcgtttt cgccagcagt
     5161 tccagatgca aacgcacccc cgccagtggc gttcgcagtt cgtgcgcgac gtcagcggta
     5221 aacaaccttt cgttatccag cgtgctggtc aggcgactga ccagatcgtt caacgccgaa
     5281 accaccgctt cgatttcgag ggtggcgctg tgaatggcaa tgggcgttaa gttgtcggca
     5341 gtgcgcgctt ccagctcttt ttgcagctcc gccagcgggc gggtgatgcg gcgtaccgcc
     5401 tgatagcaga taaatagcgt caggctgacc ataaagacgc cggggacaat caggctggcg
     5461 accgcctcgc ggatctcacg catgatgtgg cgatcgttgt tgcgattgtc gcgtagcgcc
     5521 tgctcaaaca gctgaatctg ctcggtactt tcatgccata gccagaagac gctgatcagc
     5581 tcaaacacca acaaaatggc cccgatagtc aatatcagcc gttggcgcag cgatattggt
     5641 cggcgcagaa aacgcatcag attcaattag ttttcctcat tcgcgaccag catatagcca
     5701 aagccgcgca cggtgcggat acgggatttg cccactttgt cgcgcagatt gtggatatgc
     5761 acttccaggg tgttggtcga gggttcattg tcccagttat agatgtcgtt gtagagaatt
     5821 tcccgatgta ccggactgcc tgctttgagc attaaccgtg acagcagagc atattctttg
     5881 ggcgtcagaa tcaactcttc accgcccatc catacctgac ggcgacccat attcagcgtc
     5941 agattgccaa caatcagctc actttcgccc tgattattat ggcgtcgtag cagggcgcgg
     6001 atacgggcgt gtaactcttc cagcgcaaaa ggcttcacca gatagtcatc ggcaccgaca
     6061 tccagcccgg cgattttgtc ggtcagcgta tcgcgagcgg tgaggatcag taccggcagg
     6121 gtatattttt tctgccggat acgggcgaga aaatgcagtc catcttcgtc gggtaaccct
     6181 aaatccagta ccaccaggct gtaatgaccg gcttcaaggc tttgttccgc catccgcgcg
     6241 gttgtcacgc catcgcacgc gtagccttcg gtttgcgccg ccagaatcag tccctgcaat
     6301 aacagcgtat cgtcttcaac aatcagaatt ttcatttact cactctcctg caagtttgca
     6361 gaatatcatc cgcagcctgg taatacttcg tctcaacgcc agttaatccc aatagcgtgg
     6421 agaataaatt gtcttgtgaa tagtgttgcg tttgcgcctg tttttgcagg cagttctggt
     6481 caacctgata ccgtttttga taatcctccg acagccacag cagcatcggc acctgttttt
     6541 ggctatccgg ggcgatggca taaggcagac cgtgcagata gatgccattt tcacctaacg
     6601 attcaccgtg gtcagaaaga taaaccaggc tggtggtaaa tttatcctga tgttctttca
     6661 gcagattaat cgctttatca acaatatagt cgacgtaaac cagcgtgttg tcgtaagtgt
     6721 tcaccagttg ctctttggta caggtctgga tctcattggt gtcgcaggtt ggggtaaatt
     6781 tcctgaactg cggcggatag cggttgtaat aggtcggacc gtggctgccg atggtgtgta
     6841 agacaatcac gccatcaccc tgcaggttat tgatgtactc ttccagcccg tggaacagca
     6901 cttcgtcata gcattcgccg ttgatgcact gaccaggcag attcagcgcg gtgacgttct
     6961 ggtgaggcac gcggtcgcag gcacctttac agccgccatc gttgtcattc cacagcacgt
     7021 tgatgcccgc tcgctgaatg atatccagca cgccttcctg gtgctgtgcc agctcttctt
     7081 tgtaatgctc acgcggcata tccgagaaca tgcacggtac tgaaactgcc gttgccgtgc
     7141 cgcaagatac ggtattaggg aaatagacca cgttatcttt cgccagccgc gggttagttt
     7201 cacgcgggta gccgttgagg gagaagttct ccgcccgcga ggtttcgccg acaatcagga
     7261 tggtcaaatt tttacgtttt tcgttctgca ttaacgggtt gcggtgggcg tcttcaccaa
     7321 ttcgcaccag cggtagattt gccagtcgct gatgggagta ccatgaccag ctggcaacaa
     7381 tgctgttaga ggggcttaag gatttcacca gctctttgtt attgcggaac aacgaggcgt
     7441 agtctttata aaacagtgcg gcgaccagca aaatcagtag tacagaaacc agaatattgg
     7501 ctccacggaa aagaacactg cgcagacgcg aggtggcagg tttgattttt atccagcagg
     7561 caatcagcgc agcaagcacg ccgctgaatc ccagcgttaa taacatttgc ggtgtcatca
     7621 gcgcataact ttctgccgga gtggtatcaa taatattggc aatcatcgag cggtcgatga
     7681 cgatgccgta agtcattatg aaatattgtg cagccgcgcc aaccagaata aacaggcagg
     7741 ccagtggtcg attaagccat aagaaagagc ttagtgtcag gacaatatta atcacgctga
     7801 aagcgacgac cggcatcgac aagaaaacca gtacgttatg cagcgaatcc agcggcagcg
     7861 cctgcaacac ctgtttaaaa aaggcaatat tcaggcagat agagatataa aaagcggcca
     7921 acaatagcca ggcgagtaaa ttcaaagagg gtctttttag taggcgcttc aacatcacgg
     7981 tgtttccatc gaacaaagtg cgcatatgct cgcaaagcaa aattaagcca accttaagtt
     8041 cttaaggttg gcttttatgt ttgctggatt aaggggaatt aatctttgct tattggtgca
     8101 tctaagggat acgggttttt atgtagccgg ttgtaattca gcgcatacat ggcggtgatg
     8161 accatcaggg tgacaaatga ccacataacc tctttcgctc cggaccccac cacggcccag
     8221 atgcagtaga ggaaggcaat ggtagtaact gccagatatg ccgggcgtgc tttaccaaag
     8281 tgaccgtgtc cgagcagcag taacgccgca caggtgtaaa gatatggcac cagtgtaaag
     8341 atgaccgaga cggaagaaac cagaccgaac tctttggtcg cgtttggtga aatgctgctg
     8401 agctggaaga tggtcatcaa aataccgacg ataatcagcc ccgccactgg cgtacccgct
     8461 ttatttacac gggcaaaaat cggtgggaac agtccgtcat cggcagcggc tttcgccgtt
     8521 tgacccgcca gcaacgtcca gccgcccagt gaacctaagc aacccgcagc tgcgcagaag
     8581 gaaacaatgg ccccgggggt gtcacccaac gccatccgtg cggcatcacc gaatggcgaa
     8641 gcagaaacgc gcagtgcggc attagggatc atccccataa tcgcggtggt agaaagtaca
     8701 tagcaaacgg cggcaatcaa taccccacca atggtggcga tagggacatt gcgtttcggg
     8761 tttttcacca cacctgcggc aacggaggca ctttccacac cgatgaacga ccacagcgta
     8821 acgttaaggg tactttgaat tgcaccgaag gtacccaggc cgctgacgtt ccatgccgcc
     8881 atataggttt caccacggaa ccagaaccag ccaaatacgg caatcccgac gatggggatc
     8941 agcgccagca cggtggcaac tgcctgcaca cgggtgatca ttttcggacc aacaatgttc
     9001 agcaggacga agatccacag caccacgacg caggtgatgg ttaataccaa tggatctttc
     9061 agaatcggga agaagtaact taaatatcct acgccaatga ccaccatggc gatattgccg
     9121 atccagcagg ccagccagta gaggacgttg gtttgataac cgagaaacgg gccaaagcag
     9181 cggcgggcgt aagcgtaaga accaccagga cttgggtcga ggaacgacat tttggcgtat
     9241 accatcgaga gccccagtgc accgataatc gtcaccaacc atccataaat ggcaatcccg
     9301 ccagtggagg ccaggtttgc aggtaacaga aaaacacctg accccataat attccccgac
     9361 accatcaggg tgacggggat taagcccact ttgtgagcat cagcatccga agacataatt
     9421 aaactcctgc gaaggcgagc ttcgtgacaa taaattacgt catatcatac gcctgcattc
     9481 gatagttgaa ttattatcga ccgggttatc gctgatgccg ttattaatat agctcttcgt
     9541 taaggttaaa aatcaggcga cagtgcgttc tctgtgctga ctaacataat gcagcggcgt
     9601 cataccgtag aagtctttaa agacagaaat aaagtacgac gtactgttgt agccgcagga
     9661 ctgtgatacc tgagagatat ttttaccgtc catcattaat tcatttacgg cataacgcat
     9721 gcggcaggtg gtgattattt ggctataact ggtgttttcg cttttcagct ttttctttaa
     9781 cagacttggg ctaagacata aacaactggc taccatacta agattccagt ctttgtgaat
     9841 atcgctttca ataatttgat aaacgctgtc acttacacaa ttacgtaaca tatacatcat
     9901 taatgaaagg aattttttac tgtcgagaaa acgggatagc accgtgaata ataatgcgcg
     9961 tgttcgttcc ttttctgact cagtttctgc gggcatcatg ctgtgttgcg cggcaacgcg
    10021 aaagacgtct ggcgtcaggc atggcagggt gagtatgggc gtagcgctac gttgccatac
    10081 tggtatttgc gagagatctt tattcagaaa ccggaggtaa tctttgatga tgtcgtgact
    10141 aatatgagca accaaagtgt tgttaagaga ggagacgtcg ataatattat tttcacaatt
    10201 taataacgcc atatggttag ctttaaggct aataggttct ttcccattca cccttatcca
    10261 gacatctttt tcagtcaata aaacaataca aggttggtcg ctgcaaatcc tcatacatgt
    10321 actcctgagt gcgaataaaa gaccagttct cgctaaagca aagcgatacc gagaaatggt
    10381 tgtataattg tatgacgaat ataaacatgc ataaaaataa cgagttttac taatattaca
    10441 aatttttata gtaatttctc atgtacatat ttttttacag ggtaaataac aggcaaaaaa
    10501 aagcgcgggg tggggccgcg caaaaagtat gacatttttg tttgcagcaa tttttatgaa
    10561 aacgttgcca tactcaaaag gcctaaggcc aggcggcgta atgttattta aacaattacg
    10621 ccttcagcgg aatagtggtt acgctttcac acacatagcg tggtaaatac cgtcaataat
    10681 ttcagtccct tcagtttcgt gttcaaatcc agggaaatgg tggtcccagg attgcagcga
    10741 gcgtaaataa cttacttgcg gactgttttt atcgccgaag ttttcaccag acagcagcat
    10801 cgggattcct ggcggatatg ggataactga gtttgccgcg atgcgtcctg gcagattttc
    10861 aatggatacc agttcgacat tgttgtcgac aatcgcgttg tacgcttcac gcggggtgat
    10921 ttccgccacc ggcaggccgg aataggcttc gttcaaccgt gcgccagggt tgttttcttt
    10981 cagccaggca aacatggtgt cacccagatc gtgaatcccc atgttcgcgt aagtgtcagg
    11041 atattgttca acaagttccg gcatcacctg cgccagcggt gtgttggcgt catagtggcg
    11101 tttgaaggag caaagggtgt taaccagagt cccccatttc ccacgggtta cgcccataga
    11161 gaacaggaac ataatttgga agtcagtggt gcgggtaggc acaatgccgt ggcgaccaag
    11221 ccaggcagtg accagcgccg ccggaacacc ggtttcttcc agttcaccat cttcacccat
    11281 tcccggagca aggatgctga ctttaatcgg gtcgagcata ctccagttat ccggaatatc
    11341 tttgaagccg tgccagcttt cgcccggatg cattacccag tagtcctgaa cggtggtcag
    11401 cagtttggtt ggtgcgtcag caaagtcata ggttttgccg gtttgtgggt cggtgacgac
    11461 ttctttgttc cacggtttga agaaccagct accgtcagcg gtgaactctt tatacagccg
    11521 cgccatcgcc tgacggaaat caaccgcttc gtcgatcact tcctgcgtca gtgacaggcc
    11581 gctgttgccg tccatcatcg acaccgccac gtcgttggat gcgcagatgg catacagcgg
    11641 ggaggtggtg gcatgcatca tgtaggcctg gttgaagcgg gagaagttaa tcgccccacg
    11701 accttcacgt acatgaatat aagaagcctg tgaaagcgca ttcagcagtt tgtgggtgga
    11761 gtgggtggcg aaaacggtag gaccgttgtg atcgccaggt tcgccgcgca tggcatagtg
    11821 atcggcatag atcgggttga aacgtgcata gccgtaccag gcttcgtcaa agtgcagacg
    11881 atcggaggtt ttttccagca gatcctgcgc ttctttagcg ttataacaca cgccgtcata
    11941 ggtgcagttg gtcaccacgc agtaagacgg tttttgcccg gctttgtctt tagtcagcgg
    12001 gctttcactg attttcttct gcaaggtttc aggttgcatt tcctgcggat agattggccc
    12061 gataatgccg tagcggttgc ggcttggcac catgtagacc ggtttcgcgc ctgtcagcat
    12121 caagccttgt tcgatggatt tatggcagtt acggtcaacg accacgacat cgttatcggt
    12181 catgcaagcc tgcatgatgg tgcggttaga gccggaagta ccgacgacta ccgaccagga
    12241 gcgatcggca ccaaatacgc gtgcggcata tttttcgctt tcgccaaatg cgccagtatg
    12301 gtcaagcaaa gaaccgaggg aagttcgttc gatgcccata tcggtgcgga acagattttc
    12361 tccatagtag tcatggtaga aacgtccggc gggtgttttg gtaaaaccaa cgccgccctg
    12421 gtggcctggc gctgcccagg aatattcatg gatgtcgcta tacttcatca gcgcgctgaa
    12481 cagtggcggc aacagctgct ggcggtagcg ggtcatcgcg gcaacggcgc gtccggcgat
    12541 aaagtcggtg gtatcttcca gaatccaggc gaattcatcg acaagctcca gcaggtcgcg
    12601 atccattgcg gcgagggctt tttcccgatc gcccaacagg aagaccggca cgttttgttg
    12661 gcgctcatga agcttaccga tcaattgtct gacgttttga tgttcgtccg gatgttccat
    12721 ttgatagctg aacatcaggc agtcaatggc ttcgtttgaa gagagaatgg caaaaccatc
    12781 atcaaaggag gtggatttaa tcacggtaac attttgctgg cttaaagcat ctgccagacg
    12841 ctcaacggcg ttaccgaccc aggtgtcttg atggagaaac tcgctttcaa caattaatac
    12901 tttcatcatt gcttacccgg ttatgaagga atcttcgttg cgggcaagta tcttccggcc
    12961 ttttcgggct gtaaagcgcg tgaaaaacaa acatttttga aatatttatt tttataaaca
    13021 atgcgttatg tgattatttt aaattatttt cactgcttgt tatcgacggc taaactattt
    13081 ttttggctga tactgatatc gtctttagcc gggaaacgtc tggcggcgct gttggctaag
    13141 tttgcggtat tgttgcggcg acatgccgac atatttgccg aacgtgctgt aaaaacgact
    13201 acttgaacga aagcctgccg tcagggcaat atcgagaata cttttatcgg tatcgctcag
    13261 taacgcgcga acgtggttga tgcgcatcgc ggtaatgtac tgtttcatcg tcaattgcat
    13321 gacccgctgg aatatcccca ttgcatagtt ggcgttaagt ttgacgtgct cagccacatc
    13381 gttgatggtc agcgcctgat catagttttc ggcaataaag cccagcatct ggctaacata
    13441 aaattgcgca tggcgcgaga cgctgttttt gtgtgtgcgc gaggttttat tgaccagaat
    13501 cggttcccag ccagagaggc taaatcgctt gagcatcagg ccaatttcat caatggcgag
    13561 ctggcgaatt tgctcgttcg gactgtttaa ttcctgctgc cagcggcgca cttcaaacgg
    13621 gctaagttgc tgtgtggcca gtgatttgat catcatgccg tgagtgacgt ggttaatcag
    13681 gtctttatcc agcggccagg agagaaacag atgcatcggc agattaaaaa tcgccatgct
    13741 ctgacaggtt ccggtatctg ttagttggtg cggtgtacag gcccagaaca gcgtgatatg
    13801 accctgattg atattcactt tttcattgtt aatcaggtat tccacatcgc catcgaaagg
    13861 cacattcact tcgacctgac catgccagtg gctggtgggc atgatatgcg gtgcgcgaaa
    13921 ctcaatctcc atccgctggt attccgaata cagcgacagc gggctgcggg tctgtttttc
    13981 gtcgctgctg cacataaacg tatctgtatt catggatggc tctctttcct ggaatatcag
    14041 aattatggca ggagtgaggg aggatgactg cgagtgggag cacggttttc accctcttcc
    14101 cagagaggcg aggggagcgt ccgagcatca tgaggccgaa aactctgctt ttcaggtaac
    14161 ttattaccat aaactcagat ttactgctgc ttcacgcagg atctgagttt atgggaatgc
    14221 tcaacctgga agccggaggt tttctgcaga ttcgcctgcc atgatgaagt tattcaagca
    14281 agccaggaga tctgcatgat gactgcaccc aaaattacat ttatcggcgc tggttcgacg
    14341 attttcgtta aaaatattct tggtgatgtg ttccatcgag aggcgctgaa aacggcgcat
    14401 attgccctga tggacattga tcccacccgc ctggaagagt cgcatattgt ggtgcgtaag
    14461 ctgatggatt cagcaggggc cagcggcaaa atcacctgcc acacccaaca gaaagaagcc
    14521 ttacaggatg ccgattttgt cgtggtggca tttcagattg gcggttatga accttgcacg
    14581 gtgactgatt tcgaggtctg taagcggcat ggtctggaac aaaccattgc cgatacgttg
    14641 gggccgggcg gtattatgcg cgcgctacgt accattccgc atctgtggca aatttgcgag
    14701 gacatgacgg aagtctgccc cgatgccaca ttgctcaact acgttaaccc aatggcgatg
    14761 aatacctggg cgatgtatgc ccgctatccg catatcaaac aggtagggct gtgccattcg
    14821 gtgcagggaa cggcggaaga gctggcgcgt gacctcaata tcgatccgtc cacgctgcgt
    14881 taccgttgcg ctggtatcaa ccatatggcg ttttacctgg aactggagcg taaaaccgcc
    14941 gacggcagtt acgtgaatct ctacccggaa ctgctggcgg cttatgaagc agggcaggca
    15001 ccgaagccac atattcatgg taatactcgc tgccagaata ttgtgcgcta cgaaatgttc
    15061 aaaaagctgg gctacttcgt cacggaatcg tcagaacatt ttgccgaata tacgccgtgg
    15121 tttattaagc caggtcgtga ggatttgatt gagcgttata aagtaccgct ggatgagtac
    15181 ccgaaacgct gcgtcgagca gctggcgaac tggcataaag agctgg
//
LOCUS       DAJTYD010000086        14981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTYD010000086 DAJTYD010000000
VERSION     DAJTYD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.086"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..312)
                     /locus_tag="OO585_002399"
     CDS             complement(<1..312)
                     /locus_tag="OO585_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6629960.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GK"
     gene            complement(317..502)
                     /locus_tag="OO585_002400"
     CDS             complement(317..502)
                     /locus_tag="OO585_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6629961.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(499..3138)
                     /locus_tag="OO585_002401"
     CDS             complement(499..3138)
                     /locus_tag="OO585_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6629962.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3346..4335
                     /gene="ldhA"
                     /locus_tag="OO585_002402"
     CDS             3346..4335
                     /gene="ldhA"
                     /locus_tag="OO585_002402"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6629963.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4446..4868
                     /gene="hslJ"
                     /locus_tag="OO585_002403"
     CDS             4446..4868
                     /gene="hslJ"
                     /locus_tag="OO585_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6629964.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4865..5131)
                     /locus_tag="OO585_002404"
     CDS             complement(4865..5131)
                     /locus_tag="OO585_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6629965.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5405..8929
                     /gene="nifJ"
                     /locus_tag="OO585_002405"
     CDS             5405..8929
                     /gene="nifJ"
                     /locus_tag="OO585_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6629966.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9295..10428
                     /gene="ompN"
                     /locus_tag="OO585_002406"
     CDS             9295..10428
                     /gene="ompN"
                     /locus_tag="OO585_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6629967.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10569..11003
                     /gene="uspF"
                     /locus_tag="OO585_002407"
     CDS             10569..11003
                     /gene="uspF"
                     /locus_tag="OO585_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6629968.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11560..12474
                     /gene="sitA"
                     /locus_tag="OO585_002408"
     CDS             11560..12474
                     /gene="sitA"
                     /locus_tag="OO585_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6629969.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12474..13301
                     /gene="sitB"
                     /locus_tag="OO585_002409"
     CDS             12474..13301
                     /gene="sitB"
                     /locus_tag="OO585_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6629970.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13298..14155
                     /gene="sitC"
                     /locus_tag="OO585_002410"
     CDS             13298..14155
                     /gene="sitC"
                     /locus_tag="OO585_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6629971.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14152..>14981
                     /gene="sitD"
                     /locus_tag="OO585_002411"
     CDS             14152..>14981
                     /gene="sitD"
                     /locus_tag="OO585_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6629972.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     "
BASE COUNT         3748 a         3757 c         3641 g         3835 t
ORIGIN      
        1 tttaccgttt atcccttgta catattcccc cggtttggcc cgagctacga gcttttgccc
       61 ggcgagtttc gcgatatcat ctacagacac attattttgc tttgccagtt gttggtaact
      121 cgctttacgt tcggcattaa tgtcggttac taatttctct gtttcagcat ccgttttcag
      181 cgcaaccaga taaccataaa atgtttcccc gacccgcccc tgcgttctgg cttcatccag
      241 agtcaatgcc attacattgc tgcttaccag cccaacaaga aacgcacaaa gaagtaatgt
      301 tttcttcatc atcgcctcag taaagatcgc tacgagtttc aagcagctct tcgacatctt
      361 tgtctgcctt gatgatgatc tcatgctcaa ttttaacgtt catattgata gtgatcggtt
      421 ccttgggcgc agcgacttca atacgcggcg tacaggcagc cagcataaaa gatgacgtca
      481 acgcagccag taaaattttc attgtttttc ctcacactct tttccttgcg gacagtcatt
      541 tcccggcaga cgtgcgttct gctccaacca tgcctggaga ttatcgccaa agcgtaaact
      601 gcgccacagc gtaaaaatat tctcttcatg atggtaatta aggtttaccg taccgctttt
      661 accatcaacg cgactggtac ctgtaatgtt ggcctgcatg gttaataaac cgagattatc
      721 taaattaata tttgtcgatg aacggctaat ttccatatag cgcaaccagt taattgctga
      781 acccgcagtc atattgtctt tcaccaccgc atctgcggtg tctttatcca gccgcaaggt
      841 cattggcccg ctatttgcca gccaaccatc tttcactatc catttttcgt tgtttagcca
      901 taacggcaat gcaccactaa atgccccgga catggcgaat tgtttcggat tgacggcgct
      961 aaccagttcg ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat
     1021 acgtaattgt ttcatcagta cgttaccgcc taacacatcg acgctaacat cagtcaacag
     1081 caagggttct tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc
     1141 tgtcaccaga ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg
     1201 ccaggctcca tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc
     1261 tggcatccag gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc
     1321 aggcgcagca gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca
     1381 gtcgggtggc accagcggct ggaatacagt cagtgattgt tttggccacc aggcgttacc
     1441 gcgcagacga ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg
     1501 taaatcgcct ttaaagagga aatacgtcgg atcgcgccca tcgacgctaa attttaaggt
     1561 tgatggcggt aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc
     1621 gccgctgaag gagggatgct gtgcgtcacg tacccagacg acgggcttgt ccagaattaa
     1681 tcgcggcttt tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagaggtc
     1741 ggtcagcgta atcgtgctgt catgccattc accttttcct gcgacatccc aggtggcatt
     1801 catcggtgta aaactgcctt ttccccagta gcgccactgc cagcggcccg cgtcagggag
     1861 aaaatcattc gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc
     1921 ctgcaaaatg gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa
     1981 aggccagcgg atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa
     2041 cgcgcctggc tcaaaggcca gcgttgggtc agtcagactt ccacttagct gcgcgggtaa
     2101 gcgggcatat aaaatgaggt ccgcctgttt cgcttcaccg gtcagctgca gaggcagctg
     2161 gctgttatcc atgcttaatt ttcctgggcc aaaatttagc accgcgttgc ctttacccgc
     2221 ttgcccctgg gtcagcacac tcagtcgtcc gctgatcaga gcgttctcaa gccctgcctg
     2281 ccagttgtcg actttgacac ccagtcggcc actcaaagga aaacctgcat acggccaact
     2341 ccagcgacca tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa
     2401 cggatcgccg ttatcacgtg ccagcacaat caattgcccg ctattttcct gccagtccag
     2461 ctctgcatcc accagtgacg gttcctgcgg caagtttaac gtcgcagtag catgcccact
     2521 tacaggaagt ccatccggca cgagcggcat agtaaattcc cccaccagtt taaccggcgg
     2581 ctgatattca aacgcgatga catccagttc gctgactgta agttgttgcc ctttcagctg
     2641 gccttgaaat ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc
     2701 gagagagagt tttccctgcc attcctgcca gggagaaaaa atcagtttat cgatattgat
     2761 ccaggtgtta ggcagcatgg actgccactg cgcgagggtt tttggagcag ctggcgattg
     2821 ctccgtctgc ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa
     2881 taaccagcgg ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc
     2941 caccagataa cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc
     3001 taatgcaata cgtgtcccga gcggtagcca gatgcccgcc agcgtgggaa cccacagccc
     3061 gagcgtcatc agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc
     3121 tttatattta cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa
     3181 tacgggtatt gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt
     3241 tgttgatgaa tttttcaata tcgccatggt tttcaattat atttgaaatt ttgtaaaata
     3301 tttttagtgg cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt
     3361 tatagcacaa aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt
     3421 gagctggaat tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc
     3481 gaagcggtat gtattttcgt aaacgatgac ggcagccgcc cggtgctgga agagctgaaa
     3541 aagcacggcg ttaaatatat cgccctgcgc tgtgccggtt tcaataacgt cgaccttgat
     3601 gcggcaaaag aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt
     3661 gctgaacacg ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag
     3721 cgtacccgtg acgctaactt ctctctggaa ggtctgaccg gctttactat gtatggcaaa
     3781 acggcaggcg ttatcggtac cggtaaaatc ggtgtggcga tgctgcgtat tctgaaaggt
     3841 tttggtatgc gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt
     3901 gtggagtatg tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc
     3961 ccgctgacac cggcaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat
     4021 ggcgtgatga tcgtcaatac cagtcgcggt gcattgattg attctcaggc agcaattgaa
     4081 gcgctgaaaa atcagaaaat tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat
     4141 ctgttctttg aagataaatc caacgacgtg atccaggatg acgtattccg tcgcctgtct
     4201 gcctgccaca acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc
     4261 agtatttctc agactacgct gcaaaactta agcaatctgg aaaaaggcga aacctgcccg
     4321 aacgaactgg tttaatgttg cagctcccct gcaatccagg ggagctgatt cagataatcc
     4381 ccaatgacct ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa
     4441 ccacaatgaa gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa
     4501 gtaatgacaa aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa
     4561 gcgtaaacgg taagcccgtg accagcgata aaaatccgcc agaaatcagt tttggtgaaa
     4621 aaatgatgat ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg
     4681 gtgaactgac agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta
     4741 atgaactcga taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg
     4801 cgaaccagtt aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa
     4861 tgaattaata gctgccacag ctcccggcgg caagtgactg ttcgctacag cgtttgccgt
     4921 tgggtaatgc acacatccca atcgccgtgc catccagttg acgggcaaca gaaagcgaac
     4981 cgccgatcat tgcacaattt gcttctccac tactggacat cgacgctttt aaacctggcg
     5041 ctacgtgcgc cgcagtcgcc tgctgaacag gttcactact acacgccgac aacaataaag
     5101 cggcacaccc tacccaaaac gctgctcgca tcttttcttc ctctgatctt caagccaaac
     5161 gacaccgcca taaataatag gcagcacaga gggcgacatc gagagctgtc ctgcgcgttg
     5221 ccccgcattt ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat
     5281 tacgctgatg tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga
     5341 aagattacta ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg
     5401 tcatatgatt actattgacg gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga
     5461 agttatcgcc atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg
     5521 ggccggaaac ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc
     5581 ggaagcgggt gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc
     5641 gtttacgtca tcgcagggtt tgctgctgat gatcccgaca ctgtacaaac tggcaggcga
     5701 actcacaccg tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactctctat
     5761 ttttggcgat cattccgacg tcatggcggt gcgccagacg ggttgcgcga tgttgtgtgc
     5821 ggcaaacgtc caggaagcgc aagactttgc tctcatttcg cacatcgcga cgctgaaaag
     5881 ccgcgtgcca tttattcatt tctttgatgg tttccgcacg tcccacgaaa tcaataaaat
     5941 tgtcccgctg gccgatgaca cgattcttga tctcatgccg caggccgaaa ttgatgctca
     6001 tcgcgcccgg gcgctcaacc cggaacatcc ggtgatccgt ggtacgtccg ccaatcctga
     6061 ctcttatttc cagtctcgcg aagccaccaa cccctggtac aacgcagtct atgaccatgt
     6121 tgaacaggcg atgaatgatt tctctgccgc gacaggtcgt cagtatcagc cgtttgaata
     6181 ttacgggcat ccgcaagctg aacgggtgat tatcctgatg ggctcagcca ttggcacctg
     6241 tgaagaagtg gttgatgaat tgctaacccg tggcgaaaaa gtcggcgtgc tgaaagttcg
     6301 cctgtaccgc cccttctcag ccaaacactt gctacaaact ctaccgggat ccgtacgcag
     6361 cgtggcggta ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga
     6421 tgtgatgacc gcactggcag aagcctttaa taatggcaag cgcgaaactc tgccccgtgt
     6481 cattggtggg cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt
     6541 atttgccgag ctcaacgcgg ctaaaccgaa agcgcgcttt acggttggta tttacgatga
     6601 tgtaaccaat ctgtccctgc cgttgccgga aaacacccag ccaaactcgg cgaaacttga
     6661 agccttgttt tatggccttg gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa
     6721 gattatcggt aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa
     6781 agcgggcggc ctgacggttt ctcaccttcg cgtgagcgaa cagccgattc gttccgctta
     6841 tctcatttcc caggctgatt ttgttggctg ccaccagttg cagtttatcg ataaatatca
     6901 gatggctgag cgtttaaaac ctggcggcat tttcctgctc aacacgccgt acagcgcaga
     6961 tgaagtgtgg tcgcgcttgc cgcaagaagt tcaggccgtg ttaaaccaga aaaaagcgcg
     7021 cttctatgtg attaacgcgg cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa
     7081 taccgtcatg cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct
     7141 cgcagaattg cagggtgcga ttgccaaaag ttacagtagc aaaggccagg atctggtgga
     7201 aggcaactgg caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc
     7261 ggtaaatccg cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt
     7321 gaaaaccgta accgcagcga tgctcgcagg gcttggtgat gccctccccg tttcggcgct
     7381 gccgccagac ggcacctggc cgatggggac cactcgctgg gaaaaacgca atatcgccga
     7441 agagatcccc atctggaaag aggaactctg tacccaatgt aaccactgcg tcgccgcttg
     7501 cccacactca gctattcgcg caaaagtggt accacccgaa gcgatggaaa acgcccctgc
     7561 cagcctgcat tcgctggatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca
     7621 ggtggctccg gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga
     7681 ccgtcagaat ccagagatta aagccatcaa tatgatgtcg cgcctggaac atgtcgaaga
     7741 agagaaaatc aattacgatt tcttcctcaa cctgccagaa atcgaccgta gcaaactgga
     7801 acgtattgat attcgtacat cgcagctgat tacaccgctg tttgaatatt ccggtgcttg
     7861 ctccggttgt ggcgagacgc cgtatattaa attgctgact cagctctatg gcgaccggat
     7921 gttgatcgct aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc
     7981 gtataccacc gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa
     8041 tgccgaattt ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg
     8101 tctgctggat caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga
     8161 cgctacgcca gaggttcgtc gagaacaggt cgcagcttta cgccagcaac tcaacgatgt
     8221 tgccgaagca catgaactgc tacgtgatgc agatgcactg gtggaaaaat caatctggct
     8281 gattggtggt gatggctggg cttacgatat cggctttggc ggtctggatc atgtattgag
     8341 tttgacggaa aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg
     8401 tcaggcgtcg aaagcgacac cgctgggtgc agtaactaaa tttggcgagc acggcaaacg
     8461 taaagcgcgt aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca
     8521 gatttctctc ggcgcgcagc tgaatcagac ggtgaaagcg attcaggaag cggaagcgta
     8581 tccgggacca tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc
     8641 actcagccat gaccagatgc gccaactcac agctaccggc ttctggccgc tatatcgctt
     8701 cgatcctcgt cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc
     8761 agaagcactg gaagaaacgt tacttcacga gcaacgtttc cgtcgtctga attcgcagca
     8821 gccagaagtg gcagaacagt tatggaaaga tgctgcagct gatttgcaaa aacgctatga
     8881 cttcctggca caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat
     8941 aaggattatc caattctaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg
     9001 ttggaaaatc agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc
     9061 atataacccg cgcagaataa cgtatagaaa attaaaagta taatttttat tttgtatgta
     9121 ttatcttcga tacattcacc agacttatta tcatttcaga agaattattt gattaaggtt
     9181 ttacttaagg cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt
     9241 tcactccccg gaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa
     9301 agcaaagtac tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc
     9361 gaagtttata ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat
     9421 tatttttctg ataattcagc gaaagatggc gatcagagct atgcgcgtct gggttttaaa
     9481 ggcgaaaccc aaattaacga tcaactcact ggctacggtc aatgggaata caatattcaa
     9541 gcaaacaaca ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccgggctg
     9601 aaatttgcag attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc
     9661 gaaggctgga ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac
     9721 tttatgactg gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg
     9781 gtaaatggtc tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt
     9841 caggaaggca ccaacaacgg acgtgatgtt cgccatgaaa acggtgacgg ctggggtttt
     9901 tccacaacat atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac
     9961 cgcaccaatg accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact
    10021 gctgggctaa aatacgatgc taacaatatt tacctagcaa ccatgtattc agaaacgcgt
    10081 aatatgaccc cgtttggcga cagcgattat gctgtggcaa acaaaaccca gaattttgaa
    10141 gtcactgccc agtaccagtt tgattttggt ctgcgtccgg cagtctcttt cctgatgtct
    10201 aaaggccgtg acctgcacgc tgccggtggt gcagacaacc cggcaggtgt tgatgataaa
    10261 gatctggtta aatacgccga tgttggcgcg acttactatt tcaataaaaa catgtccacc
    10321 tacgttgact ataaaatcaa cctgttggat gaagatgaca gcttctacgc tgccaatggc
    10381 atctctaccg atgatattgt cgctttaggt ctggtttatc agttctaaat cctcctgccc
    10441 gctgttatgg cgggcttttt ctgcttattc ttcctccttt gatctaaatt aaaaatgtga
    10501 actccatcat tacacaaaaa gtgtcatctg gcgttacact ttatgcggat actaaaacag
    10561 gaggttttat gaacagaacg attcttgtcc ctatcgatat ttccgattca gaattaactc
    10621 aacgcgtgat tagccacgtt gaggcagagg caaagattga cgatgcagag gttcatttcc
    10681 tgacggtaat accttcactg ccctactatg cctctctggg tttagcgtat tccgcagaat
    10741 taccggcaat ggatgacctg aaagcggaag ccaaatcgca actggaagag atcattaaaa
    10801 aatttaaact gccaaccgac agagtgcatg tccatgttga ggaaggctcg cccaaagacc
    10861 gcattctgga attggcgaag aagatccccg ctcatatgat catcattgct tcccatcgac
    10921 cggatatcac cacttatctg ctcggttcca acgccgcagc tgtagtgcgt cacgcagagt
    10981 gctccgtgct ggttgtgcgc tgacactaac gcccgcacat tgctgcgggc tttttgattc
    11041 atttcgcaaa tgtgctgaca ttttcccctc taatccgtac catacacgcc actgttttta
    11101 tatcagattt cttatgctgg gcattccggc atgacgcata ctcttctgat gccatataac
    11161 gaattgagtc gcttttaaat gtcgcaaaat caagaaatta gtaagaaaga acaatacaac
    11221 ctgaacaagt aagggcaaaa atcacaacta tctgactcat aagtatttta tttatttttc
    11281 aatatgttaa acattatcag cgacaacaat aagctacgat aacccactct ttttttgccc
    11341 catttttgcc atcaacaatt ttcctcataa agtcatttca aagcctaata gctgctctac
    11401 ttttttcctc aatatcatgt cgccagcagg aaacaaccta atccctgttt taggcctcaa
    11461 agctgttaat gataattatt ttcattttgt ataggttatg ctataaaaca tagccgttgc
    11521 tatgtttgtt ctaattttgc tcactatagg tactaaatta tgcactcaat aaaaaaagta
    11581 accatgctct tggggtgcct cgccctcacc tgctcgatcg catttcaggc aagtgcaact
    11641 gaaaaattca aggtcattac aacatttacc atcatcgcgg atatggccaa aaacgtggct
    11701 ggagatgctg cagaagtctc atccataacc aagcctggtg cagaaattca tgagtatcag
    11761 cctacccctg gcgatattaa acgtgcacag ggggcacaac tgattctcgc caatggtatg
    11821 aatctggaat tgtggttcca acgcttttac cagcatctca atggggttcc agaagtaatt
    11881 gtctcttcgg gtgtgacgcc agtagggatc accgaaggac cctatgaggg caaacctaat
    11941 ccccatgcat ggatgtcgcc agataatgct ctgatttacg tcgataatat tcgtgatgcg
    12001 ttgataaaat acgatccggc aaatgcacaa acctaccaac gtaatgccga tacttataaa
    12061 gccaagatta cccaaaccct tgcccccctg cgtaagcaga ttacggaact ccctgagaat
    12121 cagcgatgga tggtcaccag tgaaggggct ttttcttatc tcgcacgcga tttggggcta
    12181 aaagagcttt atctgtggcc gattaatgcc gatcaacaag gaacaccgca gcaggtacgt
    12241 aaggttgttg atatagttaa gaaaaatcat atcccggcag tctttagcga gagtacgatt
    12301 tccgataaac cagcgcgtca ggttgcgcgt gaaaccggcg cacactacgg cggtgtgctc
    12361 tatgtcgatt ccctgagcac agaaaacggc ccggtaccta cgtatatcga ccttcttaag
    12421 gttactacca gtacgctggt acagggaatt aaagccggga aaagggagaa ataatgatgc
    12481 aatctgcagg cattgtcgtt aatgatgtta ctgtcacctg gcgtaacggg cacacagcac
    12541 tgcgcgatgc atccttcaca gtaccaagtg gatctattgc cgctctggtt ggggtaaacg
    12601 gttccggaaa atcgacgttg tttaaagcga ttatgggatt tgtgcgtctg accagcggaa
    12661 agatatctgt tctgggtatt cccacgcgac aggcgctaca gaaaaacctg gttgcctacg
    12721 tccctcagtc agaagaggtt gactggtcat ttcctgtact ggtggaagat gtggtgatga
    12781 tgggccgcta tgggtatatg ggtatgttgc gtatagccaa aaagagggat cgtcagattg
    12841 tcaccgatgc gctggaacgt gtcgatatgg tggattttcg ccatcgtcaa atcggcgagc
    12901 tttccggtgg acaaaagaaa cgcgtctttc tggcgagagc gattgcacag cagggtgatg
    12961 tgatcctgct ggatgaacct tttaccggcg ttgatgtgaa aaccgaagcg aaaatcatca
    13021 gcctgttgag ggagttacgc gcggaaggca aaacgatgct tgtctcaacg cacaatctcg
    13081 gctcagtcac gacattttgc gattatacgg tcatggtcaa aggcaccgtc ctggcaagcg
    13141 ggccgacaga caccactttt acagccgaga acctggagct ggcttttagc ggcgtactgc
    13201 gccatgtcac acttaacggc tcggaagaaa gcattattac tgacgatgaa cgcccttttg
    13261 tggcacatcg accgtcagcg gtgcagaggg aagcaagatg aacgtgctac tggaaccctt
    13321 cagctatgaa tatatgctca atgcgatgtg ggtctcggca atggtcggcg gtctctgcgc
    13381 tttcctgtca tgctatctga tgctaaaagg ctggtcgctg attggcgatg cgctgtcgca
    13441 ctctattgtc ccgggtgttg cgggggcata tatgctgggg cttccgtttt ctctgggagc
    13501 cttcttttcc ggcggactgg cagcgggcag catgctgttt cttaaccaac gcacccgctt
    13561 aaaagaagat gccatcatcg gcctgatctt ctcgtccttt tttggcctgg ggctgtttat
    13621 ggtgtcattg aacccgacat ccgtgaacat tcagaccatc gtcctgggca atattctggc
    13681 aatcgatccg gcggatatcc tgcaactcac tatcattggc attctctcga tcatcgtgct
    13741 ttttttcaaa tggaaagatc ttatggtgac ctttttcgac gagaatcacg cccgtgctat
    13801 tggattacat ccgggcagat taaaaatcct cttcttcacc ctgctgtcgg tttcgaccgt
    13861 tgcggcgctg caaacggttg gtgcctttct ggtgatctgt ctggtggtga ctcccggtgc
    13921 aacagcatgg ttgttaaccg atcgttttcc ccgcctgctc ataattgctg taactattgg
    13981 cagtgtaacc agtttcctcg gcgcgtgggt cagttacttt ctggacggcg cgacaggtgg
    14041 aatcatcgtg gtggcgcaga cactgctgtt tcttttagca tttgtatttg cgcccaccca
    14101 tggacttttg gcaaatcgtc ggcgtgcgca taaagcgctg gaggatcgct catgatggca
    14161 ttattactgg agccactgca gttcaccttt atgagtcacg cactgctgat ttcactggta
    14221 gtttccatac cctgtgcgct gctgtcggtc tttctggtcc tgaaaggttg ggcactgatg
    14281 ggtgacgcga tgagtcatgc ggtttttcct ggcatcgtgc ttacctggat tctggggtta
    14341 cctctggcta caggggcatt cgtcgccgga gtattctgcg cggtggcgac gggatacctg
    14401 aaagacaaca gccgaatcaa gcaagataca gttatgggga ttgttttttc tggcatgttt
    14461 gccgcaggtc tgatcttgta tattgcaatc aaaccagacg tacatcttga ccatattctt
    14521 ttcggcgaca tgctcgggat aaccatcggc gatataatcc agacggtgat tattgccggg
    14581 ctggttacac ttgttattag cgtaaaatgg cgggattttt tgctgttcag ctttgattat
    14641 caacaggcgc aggtaagcgg tttgcatacg agatggctgc attatgggct gctgtgcatg
    14701 gtttccctga ccattgtggc gacgctgaaa gcagtaggta tcattctctc tatttctctg
    14761 ctcattgcgc ctggcgccat tgcagtactc ctgacacaac gctttcatat tgcgctccta
    14821 ctggcgactg gcatatcggt aatagtgtca atgactggtg tctggctgtc cttttttatc
    14881 gacagcgcac cagctccgac gattgtcgtc ttattcgcgg tcgtgttcat catgacgttt
    14941 accgttacca gcatcaacgc acgcacaaag ggaaacgccc a
//
LOCUS       DAJTYD010000087        14930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTYD010000087 DAJTYD010000000
VERSION     DAJTYD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.087"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..454
                     /locus_tag="OO585_002412"
     CDS             <1..454
                     /locus_tag="OO585_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6629973.1"
                     /translation="LQEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISI
                     ADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMM
                     TLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(495..1427)
                     /locus_tag="OO585_002413"
     CDS             complement(495..1427)
                     /locus_tag="OO585_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6629974.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1791..4577
                     /gene="polA"
                     /locus_tag="OO585_002414"
     CDS             1791..4577
                     /gene="polA"
                     /locus_tag="OO585_002414"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6629975.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4959..5591)
                     /locus_tag="OO585_002415"
     CDS             complement(4959..5591)
                     /locus_tag="OO585_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6629976.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6173..6682
                     /gene="yihI"
                     /locus_tag="OO585_002416"
     CDS             6173..6682
                     /gene="yihI"
                     /locus_tag="OO585_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6629977.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6871..8244
                     /gene="hemN"
                     /locus_tag="OO585_002417"
     CDS             6871..8244
                     /gene="hemN"
                     /locus_tag="OO585_002417"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6629978.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8434..8544)
                     /locus_tag="OO585_002418"
     CDS             complement(8434..8544)
                     /locus_tag="OO585_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6629979.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8655..10064)
                     /gene="glnG"
                     /locus_tag="OO585_002419"
     CDS             complement(8655..10064)
                     /gene="glnG"
                     /locus_tag="OO585_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6629980.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10076..11125)
                     /gene="glnL"
                     /locus_tag="OO585_002420"
     CDS             complement(10076..11125)
                     /gene="glnL"
                     /locus_tag="OO585_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6629981.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11299..12708)
                     /gene="glnA"
                     /locus_tag="OO585_002421"
     CDS             complement(11299..12708)
                     /gene="glnA"
                     /locus_tag="OO585_002421"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6629982.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13081..14904
                     /gene="typA"
                     /locus_tag="OO585_002422"
     CDS             13081..14904
                     /gene="typA"
                     /locus_tag="OO585_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6629983.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3863 a         3741 c         3850 g         3476 t
ORIGIN      
        1 tctgcaagaa gtgaatgctg cattgttcaa agagtattta ccgttattac aacaaagtga
       61 gccgaccatt aaacaaccgg taagatggaa gaacgcactc ggcgaactaa atgccaatct
      121 ggatatcagt attgccgacc cagccaaatc ttcatcatcc acaaacaaag atatcaaatc
      181 gctcaatttt gatgtgaagt taccgcttaa tgtcgtcaca gaaaccgcaa aacagcttaa
      241 tttatctgaa ggaatggatg cggaaaaagc gcaaaagcgg gctgataaac aaatcagcgg
      301 gatgatgacc ttaggtcaga tgtttcagtt aatcacgatt gacaacaata ccgcctcgct
      361 gcaactgcgt tatacaccgg gtaaagttgt ttttaacgga caggagatga gcgaagaaga
      421 atttatgtct cgtgccggac gttttgttca ttaaaatact cactcgcctc tgctcattca
      481 gaggcgagca aaatttactt gtcctgccgc tgtgatgaca tcaacgacgt cagaagtcgg
      541 tctttttcct gccacagtga gttcagccat tgctgaaaat ggcgcttaaa gcttttgtca
      601 ttgatgtagt cgccatgtaa ctcatcggca ataggctgta aatccacatg aacgacaatt
      661 cgcgtcagtt taccgcttaa catatcgaag aacggctgac ggttattgtc cggataacac
      721 agcgtaacat taagcagttt atcgaattgt ttacccagta catttagcgc catcgcaatg
      781 cctgccgcct ttggtggcaa caagttttga aaagtggagt gggtttgctg atgtttttct
      841 tgcgtgaaac gggagccttc aacgaaattg acaatggtgg tgggatgcag gcgaaacttt
      901 tcacaagaac gccgagtggt ttcaacatct ttaccacgtc gctccggatg acgtaataaa
      961 taagcgcggg aataacgctt cataaacggc atatccagcg cccagcacgc caggccaaga
     1021 aatggcaccc aggccagctg ttgcttgagg aaatatttat tcatcggaat gtgcttacga
     1081 aacagcacgc acagtacgac aatatctgcc caactacggt ggttacaaat aagcagatac
     1141 cagttcttct tacttaaccc ttccagcccg tgaacttccc attgcaggtg tgggttcaga
     1201 tgcagtaata ccgccagacc ttcacaccag caatacatca taaaatcaca aaaacgcgaa
     1261 acctttcgcc aaatgactgg cacaggtagc aatagtttta caatcccggc aatgatgatc
     1321 gggacggaac aaaaaatagt gaccaaaatg gtcaatacga tactcagcaa aagggttatc
     1381 gcagcgagta ttctcgtcat aatgaattta ttcaaaaggt tagccataga cagtgcgcta
     1441 aagaagcgca aggcgcagat tttagcagaa aacgaccgaa ataacggatg atccttaagg
     1501 agaaaaataa ttcatatcta tccacattag aaaaaatccc attatctcaa ttattaggga
     1561 tggctttatt tttaactaca tgaaaaacaa agacaaacat catactgtaa aaagcatgat
     1621 aataaattaa aagcgatgta aataatttat gcacaaagtt atccacatga cgatttgcga
     1681 gcgatccaga agatctacaa aagattttca cgaaaagcgg tgaaaaactc atgtttttat
     1741 cctgtctgtg gcatccttta cccataatct gataaacagg cacggacatt atggttcaga
     1801 tcccccaaaa tccacttatc cttgtagatg gttcatctta tctttatcgc gcatatcacg
     1861 cgtttccccc gctgactaac agcgcaggcg agccgaccgg tgcgatgtat ggtgtcctca
     1921 acatgctgcg cagtctgatc atgcaatata aaccgacgca tgcagcggtg gtctttgacg
     1981 ccaagggaaa aacctttcgt gatgaactgt ttgaacatta caaatcacat cgcccgccaa
     2041 tgccggacga tctgcgagca caaatcgaac ctctgcacgc gatggttaaa gcgatgggac
     2101 tgccgctgct ggcggtttct ggcgtagaag cggacgacgt tatcggtact ctggcgcgcg
     2161 aagccgaaaa agccgggcgt ccggtgctga tcagcactgg cgataaagat atggcgcagc
     2221 tggtgactcc aaatattacg cttatcaata ccatgacgaa taccatcctc ggaccggaag
     2281 aggtggtgaa taagtacggc gtgccgccag aactgatcat cgatttcctg gcgctgatgg
     2341 gtgactcctc cgataacatc cctggcgtac cgggcgtcgg tgaaaaaacc gcgcaggcat
     2401 tgctgcaagg tctgggcggg ctggatacgc tgtatgccga gccagaaaaa attgctgggt
     2461 tgagcttccg tggcgcgaaa acaatggcag cgaagctcga gcaaaacaaa gaagttgctt
     2521 atctctcata ccagctggcg acgattaaaa ccgacgttga actggagctg acctgtgaac
     2581 aactggaagt gcagcaaccg gcagcggaag agttgttggg gctgttcaaa aagtatgagt
     2641 tcaaacgctg gactgctgat gtcgaagcgg gcaaatggtt acaggccaaa ggggcaaaac
     2701 cagccgcgaa gccgcaggaa accagtgttg cagacgaagc gccagaagtg acggcaacgg
     2761 tgatttctta tgacaactac gtcaccatcc ttgatgaaga aacactgaaa gcgtggattg
     2821 cgaagctgga aaaagcgccg gtatttgcat ttgataccga aaccgacagc cttgataaca
     2881 tctctgctaa cctggtcggg ctttcttttg ctatcgagcc aggcgtagcg gcatatattc
     2941 cggttgctca tgattatctt gatgcgcccg atcaaatctc tcgcgagcgt gcactcgagt
     3001 tgctaaaacc gctgctggaa gatgaaaagg cgctgaaggt cgggcaaaac ctgaaatacg
     3061 atcgcggtat tctggcgaat tacggcattg agctgcgtgg gattgcgttt gataccatgc
     3121 tggagtccta cattctcaat agcgttgccg ggcgtcacga tatggacagc ctcgcggaac
     3181 gttggttgaa gcacaaaacc atcacttttg aagagattgc gggtaaaggc aaaaatccac
     3241 tgacctttaa ccagattgcc ctcgaagagg ccggacgtta cgccgccgaa gatgctgatg
     3301 tcaccttgca gttgcatctg aaaatgtggc cggacctgca aaaacacaaa gggccgttga
     3361 acgtcttcga gaatatcgaa atgccgctgg tgccggtgct ttcacgcatt gaacgtaacg
     3421 gtgtgaagat cgatccgaaa gtgctgcaca atcattctga agagctcacc ttgcgtctgg
     3481 ctgagctgga aaagaaagcg catgaaattg caggtgaaga atttaacctt tcttccacca
     3541 agcagttgca aaccattctg tttgaaaaac agggtattaa accgctgaag aaaacgccgg
     3601 gtggcgcgcc gtcaacgtcg gaagaggtac tggaagaact ggcgctggac tatccgttgc
     3661 caaaagtgat tctggagtat cgtggtctgg cgaagctgaa atcgacctac accgacaagc
     3721 tgccgctgat gatcaacccg aaaaccgggc gtgtgcatac ctcttatcac caggcagtaa
     3781 ctgcaacggg acgtttatcg tcaaccgatc ctaacctgca aaacattccg gtgcgtaacg
     3841 aagaaggtcg tcgtatccgc caggcgttta ttgcgccaga ggattatgtg attgtctcag
     3901 cggactactc gcagattgaa ctgcgcatta tggcgcatct ttcgcgtgac aaaggcttgc
     3961 tgaccgcatt cgcggaagga aaagatatcc accgggcaac ggcggcagaa gtgtttggtt
     4021 tgccactgga aacagtcacc agcgagcaac gccgtagcgc gaaagcgatc aactttggtc
     4081 tgatttatgg catgagtgct ttcggtctgg cgcggcaatt gaacattcca cgtaaagaag
     4141 cgcagaagta catggacctt tacttcgaac gctaccctgg cgtgctggag tatatggaac
     4201 gcacccgtgc tcaggcgaaa gagcagggct acgttgaaac gctggacgga cgccgtctgt
     4261 atctgccgga tatcaaatcc agcaatggtg ctcgtcgtgc agcggctgaa cgtgcagcca
     4321 ttaacgcgcc aatgcaggga accgccgccg acattatcaa acgggcgatg attgccgttg
     4381 atgcgtggtt acaggctgag caaccgcgtg tacgtatgat catgcaggta cacgatgaac
     4441 tggtatttga agttcataaa gatgatgttg atgccgtcgc gaagcagatt catcaactga
     4501 tggaaaactg tacccgtctg gatgtgccgt tgctggtgga agtggggagt ggcgaaaact
     4561 gggatcaggc gcactaagat tcgcctgaac atgccctttt ttcgtaagta agcaacataa
     4621 gctgtcacgt tttgtgatgg ctattagaaa ttcctatgca acaactgaaa aaaaattaca
     4681 aaaagtgctt tctgaactga acaaaaaaga gtaaagttag tcgcgtaggg tacagaggta
     4741 agatgttcta tctttcagac cttttacttc acgtaatcgg atttggctga atattttagc
     4801 cgccccagtc agtaatgact ggggcgtttt ttattgggcg aaagaaaaga tccgtaatgc
     4861 ctgatgcgct atgtttatca ggccaacggt agaattgtaa tctattgaat ttacgggccg
     4921 gatacgccac atccggcaca agcattaagg caagaaaatt attcgccgtc ctgcgtttct
     4981 tctacaggct gcatctcgct aaaccaggta tccagtttct gccgcagctt gtccacgcct
     5041 tgtttcttca acgaagaaaa cgtttcaacc tgcacatcac cgttaaacgc cagtacagct
     5101 tcacgcacca tattcaattg cgctttacgt gcgccgcttg ccagtttgtc cgctttggtc
     5161 agcagcacca gaacggcgat attgctgtct accgcccact caatcatctg ctgatccaaa
     5221 tctttcagcg gatggcgaat atccattagc accaccagac cttgcaggct atgacgtttt
     5281 tcgaggtatt cgccgagcgc acgctgccat ttgcgcttca tctcttccgg gacttccgca
     5341 taaccgtacc caggcaagtc aaccagacgc ttgccgtcag ccacttcaaa caggttgata
     5401 agctgggtgc gccctggggt ttttgaggta cgagccaggc ttttctggtt agtcagcgtg
     5461 ttcagcgcgc tggatttacc tgcgttggaa cggcctgcaa aagccacttc aattccggta
     5521 tcggaaggta ggtggcgaat atcaggcgca ctcatcacaa aatgcgtctg ttgataattc
     5581 aaattagtca aagcggtcgt ctccgtcagt caaagctttg tggcgattat acctgaacca
     5641 taataaaagg cggatttttc ggcgtgagcg ttgtaagtaa aaaccatacg ctttgtgaga
     5701 cattgccgat agtctttatg cgaaatagca gagaaaattc tgcgatgcat gtcaaataag
     5761 ctatataaat cagtgaattg cttttatata aaactctgaa aatgtgcaaa tactgatggc
     5821 ggttgattgt ttgtttaaag cgaaggcgta aagtagcacc catagagcga ggacgctaac
     5881 aggaacaatg actcaggatg agggtcagga gcgccaggag gcgaagacag aggattgtca
     5941 ggaagacaaa cgtccggaga cgtaattaaa cggaaatgga atcaacacgg attgttccgg
     6001 ctaaaggaaa aacagggtgt gttggcggcc tgcaaggatt gtaagacccg ttaagggtta
     6061 tgagtcagga aaaaaggcga cagagtaatc tgtcgccttt tttctttgct tgctttctgt
     6121 tagattccgc cgcaaatcga tactgaataa aacggctaaa agacgaacca ttatgaaacc
     6181 atcatcttca aactcacgca gcaaaggtca tgcaaaagcg cgtcgaaaaa cacgcgagga
     6241 gctggatcag gaagctcgtg atcgcaagcg tcagaaaaaa cgtcgtggtc atgcgccggg
     6301 cagccgcgca gcgggcggaa acaccacgtc aggcagcaaa ggccagaacg caccaaaaga
     6361 tccacgtatt ggcagtaaaa cccctattcc attgggcgtg actgaaaaag tcaccaaaca
     6421 gcacaaaccg aagagtgaga aacctatgct ttcaccgcag gcggagttgg aattactgga
     6481 aacggatgag cgtctggatg cgctgctgga acgtctggaa gcaggcgaaa ccctgagtgc
     6541 cgaagagcaa tcctgggtgg atgctaaact ggatcgtatt gatgagttga tgcagaaact
     6601 cggcctctct tatgacgatg acgaagaaga ggaagaagac gagaagcaag aagacatgat
     6661 gcgtctgctg cggggcaact aacggattgc cgccgtgggc cttcccgttc tgctgataac
     6721 ccttccggtt atatgttatc tggtgtggtt attcgttaaa ctacagcggt tgtcgcggcg
     6781 acaaaagtgg ctgcgcaacc ggctgatgac ccgaaacggg catccgccgg tacgccgtag
     6841 ccgccagaga cgccatcgga aggagtgagc atgtctgtac agcaaatcga ctgggatctg
     6901 gccctgatcc agaaatataa ctattccggg ccacgataca cctcgtaccc gaccgcgctg
     6961 gagttttcag aagacttcgg cgaacaggcg tttttacaag ccgtggcgcg ctatcctgag
     7021 cgtccattat ctctctacgt acatatcccg ttctgccata agctttgtta cttctgcggt
     7081 tgcaataaga ttgttactcg ccagcagcac aaggccgatc agtatctgga cgcgctggag
     7141 caagaaatcg tccatcgtgc accgctgttc gccgggcgtc acgtcagcca attgcactgg
     7201 ggcggcggaa cgccgacgta tctgaataaa gcgcaaatca gccgcctgat gaagctgctg
     7261 cgcgaaaact tccagttcaa tgccgatgcg gagatttcga tcgaagtcga tccgcgggaa
     7321 atcgaactgg atgtactcga tcatttacgc gccgaaggct ttaatcgcct gagcatgggc
     7381 gtgcaggact tcaacaaaga agtgcaacgt ctggttaacc gcgagcagga tgaagagttc
     7441 atctttgcac tgcttaacca tgcgcgtgag attggtttta cctccaccaa catcgacctg
     7501 atttacggcc tgccgaaaca gacgccggag agtttcgcct ttaccctgaa acgtgtggcg
     7561 gagctgaacc ccgatcgtct gagtgtcttt aactacgcgc atctgccgac catttttgct
     7621 gctcagcgca aaatcaaaga tgctgacctg ccgagtccgc agcaaaaact cgatatcctg
     7681 caggaaacca tcgccttcct gacgcaatcg ggctatcagt ttatcggtat ggatcacttt
     7741 gcccgtccgg atgacgagct ggcggtggcc cagcgtgaag gcgtgctgca tcgtaacttc
     7801 cagggctaca ccactcaggg cgataccgat ctgctgggga tgggcgtttc cgccatcagc
     7861 atgattggcg actgctacgc gcagaaccag aaagagttga agcagtacta tcagcaagtg
     7921 gatgaacaag gcaatgcgct gtggcgtggt attgcgctaa cgcgtgatga ctgtattcgc
     7981 cgcgatgtga ttaagtcgct catctgcaac ttccgtctgg attacgcccc tattgagaaa
     8041 cagtgggatt tgcacttcgc tgattacttt gcggaagatc tcaagctgct cgccccgtta
     8101 gcaaaagatg ggctggtgga tgtggatgag aagggaatac aggtgacggc gaaaggtcgc
     8161 ttgctgatcc gcaacatttg catgtgcttt gatacctatc tgcgccagaa agcgcggatg
     8221 cagcagttct cacgggtgat ttgaatagtg aatggcgctt cgtttacaaa gtagaaaacg
     8281 aaagcgccat caatgtgctg agaacaagat tgcctgatgc gctacgctta tcaggcctac
     8341 aaaacctgtt gaaaatattg cttttgtagg ccggataagg cgttcacgcc gcatccggca
     8401 tgaacaaaga gcacttcatc agcaatctga aactcaacta aacagcccaa tcattgcggc
     8461 acacagcacg gcagcaacgg cagtttgcgg tgtgtggctg tcaactgcgc cactcgatac
     8521 cagattaatt attgattcca gcataatgac tctccccgtt ttccgggcag atcatactga
     8581 acttatcgga acagtaaagc gtaaaatacc agcaatttgc gctcaataat caatctttac
     8641 acacaagctg tgaatcactc catccccagc tcttttaact tacgcgtcag ggtgttgcgg
     8701 ccccagccga gtagccgcgc cgcttcctgt ttatgcccct gcgtatgtcg caacgcggtc
     8761 gtcagtaacg tccgctccag ctctggctgc gcttcggaaa gcagattttg atgaccggaa
     8821 cgcagcgctc tgtctgccca ctgcgcaaga agcgtcgccc agctgtccgg ttgcatttgc
     8881 gaagtactct ccgcaaccgt tgattcaaac agttcgccgg gcaaatcctg aatcaacact
     8941 tcctgcccgg cggccatcac cgttagccag cggcaggtgt tttccagctg gcgcacgttg
     9001 cctggccacg ccagacgcgt cagagcagct tcggtttccg gatgcagtaa cttcgcttct
     9061 acgcccagtt cgcgcgcggc aacctgtaaa aaatggcgcg ccagacgggg aatatcttcc
     9121 cgacgttcgc gcagcggcgg cagatgaacg cggataacgt ttaggcggtg gaacagatcc
     9181 tcacggaact taccttcctg cactcgctgt tcgagattct ggtgagtggc agcgataatc
     9241 cgcacatcca ctttcaccgg cgcatagccg ccaacgcggt aaaactgacc gtctgccagc
     9301 acgcgcagca aacgcgtctg cacatccagc ggcatatcac caatttcgtc gaggaataat
     9361 gtaccgccat cggcctgttc aaaacgcccc tgacgaatgg tattcgcgcc agtaaacgcg
     9421 cctttctcgt ggccaaacag ttctgattcg atcaaatctt ttgggatagc tgccatattc
     9481 agcgcgataa acggtgcttt ggcgcgcgga ctgtggcgat gcagggcatg agcgaccagt
     9541 tctttaccgg tgccggattc gccgttaatc agcacgctaa tagaagaacg cgaaagccga
     9601 ccgataatac ggaacacgtc ctgcatggct ggcgcttcgc cgatgatatc ggtcgttggg
     9661 ccgttaagct gaatattacg cggctgctgc tgttcctggt aatgactgat agcgcgctca
     9721 accagcgcca cggcttcgtc gatatcaaac ggtttgggca gataatcaaa cgccccttgt
     9781 tgataggcgc tgacggcagc atccagatcg gaatgtgcgg tcataatgat gaccggaagc
     9841 attggatggc gctgtttaat ctgcttgagc agcgccagcc cgtccattcc cggcatacgg
     9901 atatctgaaa gcagcacatc cggcgttttg ctcgccagcg cctccagtac ttccgcgccg
     9961 ttctcaaatg tcgtacaggt taaacccgct ccagcgagcg cacgttcaag cacccaacgg
    10021 atggaactat cgtcatcgac tacccagact atccctcgtt gcataaacgt cacctttatt
    10081 tcctgatagg caggtaaacc gagaactcgg tatgacctgg ccaactggta aattcaattt
    10141 tgcctgaatg ctgatcaatc aaattacggg cgatggataa gccaagcccg gtgccacctt
    10201 cgcggccgct gaccatcggg taaaacagcg tatcctgcaa atgaggcgga atgcccggcc
    10261 cgttatcttc cacatcaatc cgcgccgcca gccggtagcg ctcgccgtgt aaggtcagtt
    10321 gaaacgcggt gcgggtacgc agaatgattt caccgccttc cggccccagc gcctgtagcg
    10381 cattgcgcac aatattcagc aagacctgtt caatttgatc cgggtcgtgc gccagttccg
    10441 gcaggctggg atcgtaatca cgaatcaacc gcacgttgtc cggcagttcc atcgacacca
    10501 gcgttaccac gcgttcagcc actttgtgaa tactttcggt aacgcgcgta ccgggcagct
    10561 gcggccccaa cagacggtcg accagatttc gcagccggtc cgcctgttcg ataatcactt
    10621 tggtatattc gagtagtgat gggtcaggta acgctttgct gagcagctgc gccgcgccac
    10681 gtaaaccgcc aagcggattt ttaatctcat gtgccaggcc gcgcactaaa tcacgggcag
    10741 caacctgctg ggcgtgctgt agctgttcct gacttaagcg gcgctggtta tccatcggag
    10801 ccatctccag caggatcatg ccgtccggca tacgctgggc cgtcacagaa aggatatgcg
    10861 agcgcccgtc gatgaccagc gtcacttcgt tatcggtaaa accttgcccc gcctccagac
    10921 tttcttgcat cagctcgata tttaatgaga agtagctcaa cagttccggt aacggtgtac
    10981 caaacaattt gcgggagctt tgggcgagca gttgttgcgc ggcagggttg gcgtaatgga
    11041 tctccaggtt gtcatcgatt aacaaaatac tgttaatcag cgagttgagg atctgcccag
    11101 catcgggctg cgtgcctgtt gccataaagc agtctcctga acaggttgca ccattttagt
    11161 gcattatagc tttttacgga taaaaagcgc gaagcatcag agaattgacg gagaaaaaag
    11221 cccatgcaga gatgggctga aagtttccac ggcaactaaa tcccggcgtt gttgcgccgg
    11281 gtagtacaac ttcaactatt agacgctgta gtacagctca aactctaccg gatgcggagt
    11341 catacgcacg cggtcatctt cttcgcgacg cagagcgatg tacgcatcaa ttgcttcgtc
    11401 agtgaacacg ccaccggctt tcaggaactc gcggtccaga tccagttcgt tcagtgcttc
    11461 ttccagagag cctgcaacct gtgggatctc tttcgcttct tctggcggca ggtcatacag
    11521 gtttttgtcc atggcttcac ccggatggat cttgttcttg ataccatcaa gaccggccat
    11581 cagcagggca gcaaagcaca ggtacgggtt agctgccgga tccgggaaac gtacttcgat
    11641 acgacgtgct ttcggagaag aaaccaccgg aatacggata gacgcagaac ggttacgcgc
    11701 agagtaagcc agcattaccg gtgcttcata gcccgggacc agacgcttat aagagttggt
    11761 ggtcgggttt gccagggcgt taatcgcttt agcgtgtttg attacgccgc caatgtagta
    11821 cagcgcctgc tcagacagac ctgcgtattt gtcgcctgcg aacaggttaa cgccgttttt
    11881 agacagagac atgtggcagt gcataccgga gccgttatca ccgaacatcg gttttggcat
    11941 aaaggtcgcg gttttaccga agcggtgcgc tacgttgtgc acaacatatt tgtagatctg
    12001 aatttcgtca gcttttttgg tcatggtatt gaagcgggta gccacttcgt tctgaccagc
    12061 agtcgctact tcgtggtgat gggcttcaac caccagcccc atctgttcca tcaccagaca
    12121 catttcagaa cgaatatcct gagccgagtc taccggtgga accgggaagt aaccgccttt
    12181 cactgccgga cggtgacctt tgttaccacc ttcgtattgg gtggaggagt tccatgcgcc
    12241 ttcgatatcg tcgatagcaa cgtgggaacc ggagatagat gatccgaaac ggatgtcatc
    12301 gaacaggaag aattcaggtt ctggcccgaa cagtacggtg tcggcaatgc cagtggaacg
    12361 caggtaatct tcggcgcgct tcgcaatgga gcgcgggtca cggtcatagc cttgcagggt
    12421 gccaggttca aggatgtcgc aacggataat cagggtggag tcggcgaaga acgggtcaat
    12481 cactgcggtg gatgcgtctg gcatcagcac catgtcggac tcgttaatgc ctttccagcc
    12541 gccaatcgag gagccgtcaa acattttgcc ttcttcgaag aattcagcat tcacctgatg
    12601 agcagggata gtgacgtgct gttctttacc tttagtatcg gtgaagcgca aatcaacaaa
    12661 cttcacttcg tgctcgttca gcatcgtcag tacgtgttca gcggacatac tttaactctc
    12721 ctggattggt catggtcgtc gtggtaacga aatctgcaat tattttggcc gtgtcgccgt
    12781 aaaaaagata aagcgaaatc tgtgccaact tttaaattgc ccctaaaagg cgttatcatg
    12841 cgcaccatcg tgcaaaaggg ctgcaccacg atgtgaatgt tgcaccaata tagtgcttca
    12901 atggaaacat taagcaccat gttggtgcaa tgacctttgg ataacccttt ttatgctccg
    12961 tgaaagcgat cacaaaggga ctctgcaata cttgtttgcg gaggatgttt gtgatcctgt
    13021 tttgtagtgc gattaatccg tgtacaataa cgcgctattt ctaatgcctg aggcaaagtt
    13081 gtgatcgaaa aattgcgtaa tatcgccatc atcgcgcacg tagaccatgg taaaaccacc
    13141 ctggtagaca agctgctcca acaatccggt acgttcgact ctcgtgccga aacccaagag
    13201 cgcgtgatgg actccaacga tttggagaaa gagcgtggga ttaccatcct cgcgaaaaac
    13261 accgctatca aatggaatga ttaccgtatc aacatcgttg ataccccggg gcacgccgac
    13321 ttcggtggtg aagttgaacg tgtaatgtcc atggtagact cagtgctgct ggtggttgac
    13381 gcatttgacg gcccgatgcc gcaaacgcgc ttcgtaacca aaaaagcgtt tgcttacggc
    13441 ctgaagccga ttgttgttat caacaaagtt gaccgccctg gcgcgcgtcc tgattgggtt
    13501 gtggatcagg tattcgatct gttcgttaac ctcgacgcga ccgacgagca gctggacttc
    13561 ccgatcgttt acgcttctgc gctgaacggt atcgcgggtc tggaccacga agatatggcg
    13621 gaagacatga ccccgctgta ccaggcgatt gttgaccacg ttcctgcgcc ggacgttgac
    13681 cttgacggtc cgttccagat gcagatttct cagctcgatt acaacagcta tgttggcgtt
    13741 atcggcattg gccgcatcaa gcgcggtaaa gtgaagccga accagcaggt cactatcatc
    13801 gatagcgaag gcaaaacccg caacgcgaaa gtcggtaaag tgctgggcca cctcggtctg
    13861 gaacgtatcg aaaccgacct ggcggaagct ggcgatatcg ttgcgatcac gggccttggc
    13921 gaactgaaca tttctgacac cgtttgcgac acgcaaaacg ttgaagcgct gccggcactc
    13981 tccgttgatg agccgactgt ttctatgttc ttctgcgtta acacctcgcc gttctgcggt
    14041 aaagaaggta agttcgtaac gtctcgtcag atcctggatc gtctgaacaa agaactggta
    14101 cacaacgttg cgctgcgcgt agaagaaacc gaagacgctg atgcgttccg cgtttctggt
    14161 cgtggcgaac tacacctgtc tgttctgatc gaaaacatgc gtcgtgaagg tttcgaactg
    14221 gcggtatccc gtccgaaagt tatcttccgt gaaatcgacg gtcgtaaaca agagccatat
    14281 gaaaacgtga ctctggacgt tgaagaacag catcagggtt ctgtaatgca ggcgctgggc
    14341 gaacgtaaag gcgacctgaa aaacatgaat ccagacggta aaggccgcgt acgtctcgac
    14401 tacgtgatcc caagccgtgg tctgattggc ttccgttctg agttcatgac catgacttcc
    14461 ggtactggtc tgctgtactc caccttcagc cactacgacg acgtacgtcc gggtgaagtg
    14521 ggtcagcgtc agaacggcgt actgatctct aacggtcagg gtaaagcggt cgcgttcgcg
    14581 ctgttcggtc tgcaggatcg cggtaagctg ttcctcggtc acggtgcaga agtttacgaa
    14641 ggtcagatta tcggtattca tagccgctct aacgacctga ctgtaaactg cctgaccggt
    14701 aagaaactga ccaacatgcg tgcttccggt actgacgaag ccgttgttct ggttccgcct
    14761 atccgcatga ctctggaaca agctctggag ttcatcgatg atgacgaact ggtagaagtg
    14821 actccgacct ctatccgtat tcgtaaacgt cacctgacgg aaaacgatcg tcgtcgcgcc
    14881 aaccgcgcac cgaaagacta ttaatttcgt tttttagtta taaaaaacct
//
LOCUS       DAJTYD010000088        14881 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTYD010000088 DAJTYD010000000
VERSION     DAJTYD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.088"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..386
                     /locus_tag="OO585_002423"
     CDS             <1..386
                     /locus_tag="OO585_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6629984.1"
                     /translation="KEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQK
                     PYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQM
                     VYEIYGMWIDDMNDEQIAMLNARLS"
     gene            438..1373
                     /gene="ttcA"
                     /locus_tag="OO585_002424"
     CDS             438..1373
                     /gene="ttcA"
                     /locus_tag="OO585_002424"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6629985.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGIPHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1502..2875)
                     /gene="dbpA"
                     /locus_tag="OO585_002425"
     CDS             complement(1502..2875)
                     /gene="dbpA"
                     /locus_tag="OO585_002425"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6629986.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3321..4304)
                     /gene="zntB"
                     /locus_tag="OO585_002426"
     CDS             complement(3321..4304)
                     /gene="zntB"
                     /locus_tag="OO585_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6629987.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4559..5791
                     /gene="dgcM"
                     /locus_tag="OO585_002427"
     CDS             4559..5791
                     /gene="dgcM"
                     /locus_tag="OO585_002427"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6629988.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5812..6375)
                     /gene="smrA"
                     /locus_tag="OO585_002428"
     CDS             complement(5812..6375)
                     /gene="smrA"
                     /locus_tag="OO585_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6629989.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6705..7613)
                     /locus_tag="OO585_002429"
     CDS             complement(6705..7613)
                     /locus_tag="OO585_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6629990.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            7789..9089
                     /gene="abgA"
                     /locus_tag="OO585_002430"
                     /pseudo
     CDS             7789..9089
                     /gene="abgA"
                     /locus_tag="OO585_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9089..10534
                     /gene="abgB"
                     /locus_tag="OO585_002431"
     CDS             9089..10534
                     /gene="abgB"
                     /locus_tag="OO585_002431"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6629991.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            10571..12097
                     /gene="abgT"
                     /locus_tag="OO585_002432"
                     /pseudo
     CDS             10571..12097
                     /gene="abgT"
                     /locus_tag="OO585_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12108..12623
                     /gene="ogt"
                     /locus_tag="OO585_002433"
     CDS             12108..12623
                     /gene="ogt"
                     /locus_tag="OO585_002433"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6629992.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            12818..13570
                     /gene="fnr"
                     /locus_tag="OO585_002434"
     CDS             12818..13570
                     /gene="fnr"
                     /locus_tag="OO585_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6629993.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            13722..14672
                     /gene="uspE"
                     /locus_tag="OO585_002435"
     CDS             13722..14672
                     /gene="uspE"
                     /locus_tag="OO585_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6629994.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(14722..>14881)
                     /locus_tag="OO585_002436"
     CDS             complement(14722..>14881)
                     /locus_tag="OO585_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR6629995.1"
                     /translation="IGGVIEQYNIPLSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGI
                     YFLGGEEQ"
BASE COUNT         3772 a         3732 c         3683 g         3694 t
ORIGIN      
        1 tgaaggagca atacaaactg accgggcatc atcgcaaaag cgaaatcact ttttatcatc
       61 gggagtacgg cagaactgaa aagcaaaaac tgcattttgt tttcatgccc agggtgtgta
      121 acgaaaaaca gaaaccttat tactcggtaa gcagtttggg tgcaagatgg aatgcagcag
      181 taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac gcgacatact tttgcctgct
      241 ggctgttgac ggcaggagcg aacccggcat ttatagccag ccagatgggg catgaaactg
      301 cgcaaatggt gtatgaaatt tacggtatgt ggattgatga catgaacgac gaacagatag
      361 ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc cccaatttgc cccatttagt
      421 accagagaac tgaaataatg caagaaaatc aacaaattac aaagaaagaa caatacagcc
      481 tgaacaaatt acaaaaacgt ctacgtcgta acgtgggcga agccattgct gacttcaata
      541 tgattgaaga aggcgatcgc atcatggttt gcctctcagg gggtaaagac agctatacca
      601 tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat caatttttcg ctggtggctg
      661 ttaacctcga tcaaaagcaa ccgggcttcc cagaacacgt tctgcccgtg tatcttgaaa
      721 agctgggcgt tgagtacaag attgtcgaag agaacactta cggtatcgtg aaagagaaga
      781 ttccagaggg caaaaccact tgctcactgt gttctcgcct tcgtcgcggt atcctttatc
      841 gtaccgcaac ggaactgggg gcgacgaaga tcgcgctggg tcaccatcgt gacgatatcc
      901 tgcagacgtt gttcttaaat atgttctacg gcggcaagat gaaaggtatg cctccgaaac
      961 tgatgagcga tgatggcaaa catatcgtta ttcgtccgct ggcctactgc cgcgagaaag
     1021 atattcagcg atttgccgat gcaaaagcgt tcccgattat tccgtgcaac ctgtgcggtt
     1081 cacagcctaa cctgcaacgt caggtgattg ctgacatgtt gcgtgactgg gataaacgtt
     1141 atccagggcg tatcgagacg atgttcagcg cgatgcagaa tgtggtgccg tcgcatctgt
     1201 gcgataccaa cctgttcgat ttcaaaggca tcccccacgg ttctgaagtg gttaacgggg
     1261 gtgatctggc gtttgatcgc gaagagatcc cactacaacc ggcgggctgg cagccagaag
     1321 aagatgaaaa tcagttggat gagttacggc tgaatgtggt tgaagtgaaa taaccaggat
     1381 agcgcccgat gcgcaagcgt atcgggctac tcttatggag gccggataag acgcggccaa
     1441 cgtcgcatcc ggcaatgccg aagaagatgt ttactcttgc acccggcaat tcaacatttc
     1501 attattttaa taaccgcacc cggcacgttt ttcctttaat cttcccgccc tgtaactgtt
     1561 tccatgcttt atgagcaaca gcctgacgga ccgcgacata gacatgcgcc ggatgcacgg
     1621 cgattttgcc aatatctgcg ccatcaagcc cgatatctcc tgtcagtgca cctaatacat
     1681 cacccgggcg cattttggct tttttcccgc catcgataca caacgttgcc atttctgctt
     1741 ccagcggcac aatggaacta ttagctggcg gcgtttgcca gttaagtttt atctgcaaca
     1801 tgtcagaaat gatattggcc cgctgtgctt cttccggagc acagaaactg atcgccagac
     1861 cgctatttcc tgcacgagct gtacggccga tgcgatgtac atgaacttca gggtcccacg
     1921 ccagctcaaa gttcaccacc agctcaagcg atttaatatc cagaccacgc gcagcaacat
     1981 cagtcgcaac cagtacacgg gcgctaccgt tagcaaaacg taccagggtc tgatcgcgat
     2041 cgcgttgctc caaatcgccg tgtaatgaca atgcactttg ccctacttca ttcagcgcgt
     2101 cgcagacagc ctggcaatct tttttggtat tgcaaaacac cacgcaagag gatggctgat
     2161 gcaagcttaa taaccgttgc aacagaggaa ttttgccttt gctggatgtc tcataaaatt
     2221 gttgttcaat gggtggcaaa gcatctgttg agtcaatttc aatcgccaaa ggatcgcgtt
     2281 gcactcgtcc gctgattacg gcgatggctt ccggccaggt tgccgaaaac agaagcgtct
     2341 gtcgagatgc aggcgcaaaa cggatgacat catcaatggc atcgctaaat cccatatcca
     2401 gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc atccagtgat accgtgcctt
     2461 tttgcaagtg atccagcaaa cgacccggcg ttgccacgat aatatgcggc gcatgttgca
     2521 acgaatcacg ctgcataccg aacggttgac caccgcacaa cgtcaaaatt ttggtatttg
     2581 gcagaaaacg cgccagccga cgcaattcac ctgccacctg atccgccagt tcacgcgtag
     2641 gacacagcac taaagcctgg gtttgaaata gcgacgcatc aatttgctgt aacaagccga
     2701 ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg cacgcgaaca tcttttccgg
     2761 caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat agttaaataa cccaactcat
     2821 taaggttcgt gagttgggcg ggaggcaaaa cattcagggt agaaaaagcg gtcacaatct
     2881 attctcgtgg tcatcgacgc aaagttagca ggcgcgtatc ctcgcagatc tacgcgggtg
     2941 gtgggtcagg catgggttgc gggcgaggga tcggatcggg cactggaaca ggatcgccag
     3001 gaatcggttc agggacagga atttgcaaat aaataagtgt cgtcatattt ccctccggtc
     3061 attgggtgga ctcttaaagg gtagacgctg ataaataaca ggcaaaaaaa agccgactca
     3121 tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga
     3181 caatatggag cgcaacgccc atcgcttgac gttgcattca cctgcaaggg agatattgcc
     3241 ctgaatgggt agagagttta ttgacttcgc tcaaactttg cggcgttttt gtatacagac
     3301 agccggaaaa attgcttttg ttacaaccat ttactacgat gcaaccataa agcaacacca
     3361 ccaataagaa caactaacag aatacaaaaa attgaaaatc cgaattgcca cccgccgcca
     3421 gggatcccac caaggttgac gccaaataac cctgtcagaa aggtactggg taaaaagacc
     3481 attgccatca atgacattgt ataggtacga cgagctaaat tttcctgcat cacctgagcg
     3541 atttcatccg ccatcacgcc agtccgtgct atacaggcgt cgatttcgtc aagaccgcgc
     3601 ccaaggcgat cggcaatatc ctgcatccga cggcgttggt catcgctcat ccacggcaaa
     3661 cgttcactgg caagacgagc ataaacatca cgttgcggtg ccatatagcg acgcatcacg
     3721 attaattgtt tgcgcagcag agccaggaat ccacgcggtg gaatttgctg atcaaggaga
     3781 ttatcttcaa ggtcgataat tttatcgtgc agctgctcga taaattcgct ggaatgatcg
     3841 gtcaacgcat cgcacacatc caccagccac cctccgcaat cggtcggacc cgtgccctct
     3901 tccagatcgc tcaccacatc gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt
     3961 aaccgcccgt ccatatatac acgcatggcg accagttgat cggggcgttc atcggtgctg
     4021 ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga gacggctgac tcggggccgt
     4081 gtgctctcgc ctgccagcgc atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc
     4141 cattgggcgc tatcatggtg tacataatta aggtggagcc agcagggatg cgcttcatca
     4201 atcacatctg tattttccag cggtttaacg ccgcctctac catccagcat ccaggcaaat
     4261 actgcatccg ggacattaac gtccgatccc ttaatcgctt ccacagtgcc tccatcatca
     4321 acgcattatt ttgtagtcta gccttctggc cctgttacgc aacatctcat caccccatta
     4381 ccctgaaatg attaataaaa ttctgtctaa attgaataca aaaagcaaaa tgcttttccg
     4441 tatacaaacc gtgtgaagtg ttaaatagcg tctatcatta tcagaattat ctgatcatat
     4501 gacgtggctt ttttgcgatc ggatagcaac aaaaattgat aaaaataacg ggatctcaat
     4561 gattacgcac aacttcaata ccctggactt actcaccagt cctgtctgga ttgtttcgcc
     4621 ctttgaggaa cagttaattt atgccaatag cgcggcgcga ctgttgatgc aagacctcac
     4681 gtttagtcag ctacgaaccg gaccctattc cgtctcctca caaaaagaac tgccgaaata
     4741 cctctccgat ctgcaaaacc aacacgatat tatcgaaatc ctcactgttc agcgtaaaga
     4801 agaggaaaca gcattaagct gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt
     4861 gattattttc gaaggtatcg aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc
     4921 aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt tttctgacta actctgcacc
     4981 aatgttgttg attgacccct cacgagatgg ccaaatcgtc gatgctaacc tcgccgcgct
     5041 caatttctac ggttataacc atgaaacgat gtgccagaaa catacctggg aaattaatat
     5101 gctcgggcgt cgcgtcatgc ctatcatgca tgaaatttcg catttacccg gcggtcataa
     5161 acctttgaat tttgttcata aactggcgga tggatctact cgtcatgtgc agacctatgc
     5221 cgggccgatt gaaatttatg gcgacaagct catgttatgt attgtgcatg atattactga
     5281 gcaaaaacgg ctggaggagc agctggaaca tgctgctcac catgacgcga tgaccggatt
     5341 actgaatcgg cgacagtttt atcacattac ggaaccaggc caaatgcagc acctcgccat
     5401 cgcccaggat tacagcttgt tgctcatcga caccgatcgt tttaaacaca ttaacgatct
     5461 ctatgggcat tctaaaggtg atgaggtgtt atgcgccctc gcccgcaccc tcgaaagttg
     5521 cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa gagtttgtct tattgctacc
     5581 aagaacccca ctggataccg cgctttcgct ggctgaaact atccgtgtaa gcgtggccaa
     5641 agtgagtatt tcgggcttac cacgttttac cgtcagcatt ggtgtggcgc atcacgaagg
     5701 aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa
     5761 tgatggacgc aaccgcgtgc tggcggcata agccgcggat gcgtctcgag atcaacggct
     5821 gcgcttagcg tggcgctccc agttttcttg cttcgcctgc gccgttttac gtagtgcgac
     5881 gtaacacgcc ccgctgccgc catgatgcgg tagcgcggtg caatatgcct gaacatcatc
     5941 aaattcggtc agccagcgcg ccacatagct gcggacaata ttggcatgcg atttatcttc
     6001 ccgcccttta ccatgaataa tcagcacatt acgcaaacca tccgccaggg cttggtgaat
     6061 gaaactgaac accattttgc ggcactcttc caccggctgg cgcaaaagat tcaggctcgc
     6121 ctgttgcgga tatttaccac tgcgcagctt atccagcacc ccatgttgca acccttcccg
     6181 ccgaaactcc agcggctgac ttagcgggat gatgtcgaga aatccggtgg tgaggaaatt
     6241 atcaagctgc agcgtgtcga tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg
     6301 gacatctgta gcacgtttca gcggctggac atcttccatg gcgtcaagaa acagcgattt
     6361 gtcgtcaagg ttcatgttac atcctcccgc aattaagagc gcgatatgat aaccagaccg
     6421 ggtcggtcca acaacgtatt accaaaaatt ccagtaataa gttccaaata ttgccgatat
     6481 tttaagcaaa atgcttatgc atgattattc attcacgata ttaataatgt aacttatatt
     6541 ttcgtgaaat ctgtcactga agaaaattgg caactaaagg ttaaaaccgt tataacacag
     6601 tcaccggcgc agaggagaca atgccggact taagacgcgg atgcactgct gtgtgtactg
     6661 tagagtctgg cggatgtcga cagactctat ttttttatgc agttttaact ttgcagatag
     6721 ccgcattctc gccggaattg cgtgattaat gacggggtca gtggtgtctg gcgactatca
     6781 cgccgctgaa tcaaataata ggccgctttc ggtaaaattt cgctaaccgg caacatcacc
     6841 agcccctgtc cgtgcaaggg atcgcagccc atttcttcag gcagtatgct gagaaaatcg
     6901 ctttttgcca ccagactgat acaggctgag aacgtctcgc agactacacc gacctgtggc
     6961 gtttgcgcct gatcgtcaag caattcactc aactgtttgt agtaactgcc gtgtggcgtc
     7021 ggcattgtcc agctgtaatc cagtaactgt ttgatcgaac gggcaccaat ggcggggtgt
     7081 cccgggcggc aaaagatcgc gaattgcttt tccagtaatt tctcaaaagt aaattcgtgg
     7141 tcgtacggtc cctgataata ggtattgatg gtgaaatcca attctccctg acgcaattca
     7201 ttaatcatcg acaccagttg cccttccata atgcgtactt ttacctgcgg atgctgctga
     7261 tgaaaacgag atatgacagc tggcatcaga ctgcgggaaa tactggcccc catgccgata
     7321 ttaatctgcc ctgccagttg cccttgtcgt tggcgaatat cctcttgggc tgcgcgcagc
     7381 tcttcaagaa taagactggc gtgctgataa aaactttcac cggcatcagt taacgtcacg
     7441 cctttactac ggcgaaaaaa gagttgcgcc gctaatcctt cttccaactc ctgaatagat
     7501 ttactcagtg ccggttgcga catattcaac attcggctcg ctccgcgaat gctgccctga
     7561 cgagccactt caacaaaagc acgaatttga tgaattttta cctgaaaagc catgacgcca
     7621 ccgataaccg ttatttatca gaccaaagaa actggcatct actttaatgc agatgattgt
     7681 gtcagggtaa tttatgaacg gttaaaactg tgaaaaatca gttagtgata agtaaaaact
     7741 atcgctacgt gaaccgggtc acacttttta ctgatgacgg gaaaggttat ggagtctttg
     7801 aatcaatttg ttaattcgct tgccccaaaa ttatcgcact ggcgacgtga ttttcatcac
     7861 tatgcagagt ctggctgggt ggaattccgc actgccaccc ttgttgcgga agaattgcag
     7921 cagctcggct attcactggc gctgggccgc gaagtagtta atgaaagtag ccggatggga
     7981 ctacctgatg aactcactct acaacgcgaa ttcgagcgcg ctcgtcaaca gggtgcgcta
     8041 gcacaatgga ttgcggtttt tgaaggtggt ttcactggta tcgtcgccac cctggatacc
     8101 ggtcgccccg gtccggtgat ggctttccgt gtcgatatgg acgcgctgga tctcagtgaa
     8161 gagcaggatg tcagccatcg cccctaccgc gacggttttg cgtcatgtaa cgccggaatg
     8221 atgcatgcct gtggtcatga tggacatacc gccattgggc ttgggctagc gcataccctt
     8281 aaacaattcg agtccggact acatggcgtc atcaaactga tttttcagcc tgcagaggaa
     8341 ggtacgcgtg gcgcgcgggc gatggtcgat gcaggtgtcg tagatgatgt tgattatttt
     8401 actgccgtgc acattggcac tggcgtacct gcgggcaccg tggtgtgcgg cagtgataat
     8461 tttatggcaa ccaccaaatt tgacgcgcac ttcaccggta ccgccgctca cgcaggcgca
     8521 aaaccagaag acggtcacaa tgccttgttg gcggcagcac aagccactct tgcactgcat
     8581 gcaatcgccc cggagcttcc agagtaaacg tgggcgttat gcaggcagga agcggtcgta
     8641 acgttgttcc tgcctcggcg ttgctgaaag tggaaacacg cggggccagc gacgtcatta
     8701 atcaatatgt ttttgaccgt gcacaacaag cgattcaggg cgcagcaacc atgtatggtg
     8761 tcggcgttga aactcgtctg atgggtgcag ctaccgccag ttctccttcg ccgcaatggg
     8821 tcgcatggtt gcaaagtcag gcggctcagg tcgcgggggg caatcaggcc attgaacgtg
     8881 ttgaagcgcc tgcgggttcc gaagatgcca cattaatgat ggcccgcgtg cagcaacatc
     8941 aagggcaagc ctcctacgtg gtgtttggca cacagctggc ggcaggtcat cacaacgaaa
     9001 aattcgattt tgacgagcag gttctcgcta ttgccgtcga aacgctggcg cgcaccgcgc
     9061 tcaattttcc ctggacgcga ggtatctgat gcaggaaatc tatcgtttta tcgacgatgc
     9121 gattgaagcc gatcgccaac gttataccga tattgccgat caaatctggg atcatccaga
     9181 aacacgtttt gaagagttct ggtcagcgga gcatctggct tcggcgctgg aatctgcagg
     9241 cttcaccgtt acccgcaacg taggctatat cccaaatgcc tttattgctt cgtttggtca
     9301 aggcaaaccg gttatcgccc tgctgggaga atatgacgcc ctggcaggtt taagtcagca
     9361 agcaggttgc gcgcaaccta catccgtgac gcccggtgaa aatggtcacg gttgcggaca
     9421 caatttgctg ggaaccgccg cctttgccgc tgcaatagcc gtcaagaaat ggctggaaca
     9481 atatgggcaa ggcggcacgg tgcgctttta tggttgtcct ggcgaagaag gcggctcggg
     9541 taaaacgttc atggttcgcg agggggtatt tgatgatgtg gatgcggcac tcacttggca
     9601 cccggaagcc tttgccggta tgttcaatac ccgcacgctg gcaaacattc aggcatcatg
     9661 gcgctttaaa gggatcgcag cacatgccgc gaatttccct catttgggac gcagcgccct
     9721 tgatgccgta acgttgatga ccactggcac caacttcctc aacgaacata ttattgaaaa
     9781 agcgcgcgta cactatgcca tcacaaatag cggcgggatc tcgcccaacg tggtccaggc
     9841 gcaggcagaa gtgctttatc ttatccgcgc ccccgaaatg accgacgtgc agcatattta
     9901 tgatcgggtc gccaaaatcg ccgaaggtgc ggcattgatg accgaaacca cggttgaatg
     9961 ccgcttcgac aaagcctgtt ccagttatct cccgaatcgc accttagaaa atgccatgta
    10021 ccatgcccta tcccattttg gtaccccgga atggaactcc gaagaactgg cttttacgaa
    10081 acaaattcag gctacgctcc cccccaacga tcggcaaaac agtctgaata atatcgccgc
    10141 aaccggtggc gaaaacggca aggcttttgc actacgtcat cgtgaaacgg tactggcgaa
    10201 tgaggtcgct ccatatgccg ccaccgataa cgtgcttgcg gcatcgactg atgtcggcga
    10261 cgtcagttgg aaactgcctg ttgcccagtg tttcagcccc tgttttgccg tcggtacacc
    10321 gctacatacg tggcaactgg ttagccaggg gcgaacatct attgctcata aaggaatgct
    10381 gctggcggcg aaaactatgg cagcaaccac actcaatctc ttcattgatt cagggctatt
    10441 gcaagaatgc caacaagagc atcagcaagt tacggacacg caaccgtatc actgccctat
    10501 cccaaaaaac gtgacactgt catctttaaa ataacaacaa caacgcaaac acaacaaccg
    10561 aggaatgccc atgagtatgt catccatacc gtcgtcctcc taatccggga agctctatgg
    10621 ctgggtcgaa agaattggta acaaggttcc ccatcctttt ttgctcttta tctatttgat
    10681 tatcgtactc atggtgacga cggcaatttt gtcggccttt ggcgtcagtg cgaaaaaccc
    10741 gaccgatgga acgccggtgg tggtgaaaaa cctgctcagt gtggaaggat tacactggtt
    10801 tttacccaat gttattaaaa actttagcgg ttttgctcca cttggtgcga tcctggcgct
    10861 ggttttaggt gccggtctgg cggagcgcgt cggcttactg ccagcgctaa tggttaaaat
    10921 ggcatcgcat gttaatgtcc gctacgccag ttatatggtg ctgtttattg cttttttcag
    10981 ccacatttct tccgatgcgg cgttagtgat catgccaccg atgggtgcgc tgatttttct
    11041 ggcggtgggc aggcatccag ttgcaggttt actggctgcc attgcaggcg taggttacgg
    11101 ctttacggct aatttactga ttgtcacaac cgacgtgttg ctgtcgggga tccgcacgga
    11161 agcggcggct gcgttcaatc cgcaaatgca cgtcagtgta attgataact ggtattttat
    11221 ggccagctcc gtagtcgtac tgacgattgt tggcggcctg ataaccgaca aaatcatcga
    11281 gccacggtta ggtcaatggc agggaaacag cgatgagaaa ctgcagacat tgaccgaaag
    11341 tcagcgtttt ggtttacgca tagcaggtgt cgtatcgcta ctttttattg ctgcgatcgc
    11401 gctgatggtg atcccggaaa acgggatatt gcgcgatccg attaatcaca ccgtgatgcc
    11461 atcacccttt attaaaggta tcgtgccact aatcattctt tttttctttg tggtttcgct
    11521 ggcttatggc atcgctaccc gcacaattcg acatcaggcg gatttaccgc atttaatgat
    11581 tgaaccgatg aaagagatgg cgggatttat cgtgatggtt tttcccctcg cccagtttgt
    11641 cgccatgttt aactggagca acatggggaa attcatcgcc gtggggctga ccgatatact
    11701 ggaaagttca gggcttagcg gcatcccggc gtttgtcggt ctggcgttgc tttcctcttt
    11761 cttatgcatg tttattgcca gtggttccgc aatctggtcg attctggccc ccattttcgt
    11821 accaatgttt atgctacttg gctttcaccc ggcatttgcg caaatcctct ttcgtattgc
    11881 cgactcatcc gtattgcctt tagcgccggt atctcctttt gttccactgt ttcttggatt
    11941 cctgcaacgc tacaaaccag acgcgaaact gggtacttac tattcgttag tcttgcccta
    12001 tccgcttatc tttttggtgg tatggctgct gatgttgctg gcgtggtatc ttgttggtct
    12061 gccgataggt ccggggattt acccacgttt gtcttaagag agaacggatg ctgagattac
    12121 ttgaagaaaa aattgccacg ccactgggtc cactgtgggt gatttgcgat gagcaatttc
    12181 gcctgcgggc ggttgaatgg gaagagtaca gcgaacgcat ggtgcagctg ctggacatcc
    12241 attatcgcaa agaaggctat gagcgcattt ctgccaccaa cccaggcggt ttaagcgaca
    12301 agcttagtga ttattttgcc ggtaatctta gcattattga tacgcttccc actgccacag
    12361 gggggacgcc atttcagcgc gaagtctgga aaactctacg cactatcccc tgcgggcagg
    12421 taatgcatta cggccaactg gctgaacaat tgggccgccc tggcgcggcg cgtgccgttg
    12481 gtgcggcaaa cggatcgaat cccatcagca tcgtcgtacc ttgccatcgg gttattggcc
    12541 gaaacggcac catgaccgga tatgcaggcg gagttcagcg aaaagagtgg ttattgcgcc
    12601 acgaaggtta tcttttgctg taaacattaa acaatttgtg ccagcttgtt cacactttta
    12661 tgtaaagtta cccttaacaa cttaatggtt ttcaaataga tagacatata tttacatcta
    12721 atatcggaat tctctgctgt taaggtttgc ttagacttac ttgctcccta aaaagatgtt
    12781 aaaattgaca aatatcaatt acggcttgag cagacctatg atcccggaaa agcgaattat
    12841 acggcgcatt cagtctggcg gttgtgctat ccattgccag gattgcagca tcagccagct
    12901 ttgcatcccg ttcacactca acgaacatga gcttgatcag cttgataata tcattgagcg
    12961 gaagaagcct attcagaaag gccagacgct gtttaaggct ggtgatgaac ttaaatcgct
    13021 ttatgccatc cgctccggta cgattaaaag ttataccatc actgagcaag gcgacgagca
    13081 aatcactggt ttccatttag ctggcgacct ggtgggattt gacgccatcg gcagcggcca
    13141 tcacccgagc ttcgctcagg cgctggaaac ctcgatggta tgtgaaatcc cgttcgaaac
    13201 gctggacgat ttgtccggta aaatgccgaa tctgcgtcag cagatgatgc gtctgatgag
    13261 cggtgaaatc aaaggcgatc aggacatgat cctgctgttg tcgaagaaaa atgccgagga
    13321 acgtctggct gcattcatct acaacctgtc ccgtcgtttt gcccaacgcg gcttctcccc
    13381 tcgtgaattc cgcctgacga tgactcgtgg cgatatcggt aactatctgg gcctgacggt
    13441 agaaaccatc agccgtctgc taggtcgctt ccagaaaagc ggtatgctgg cagtcaaagg
    13501 taaatacatc accatcgaaa ataacgatgc gctggcccag cttgctggtc atacgcgtaa
    13561 tgttgcctga tttttccgca taactcacta tccttctgtc atatcattaa atttttctga
    13621 tttattgatc tggcagaagg ttcatcactg tttcattcac cagatatggg ttaatctttt
    13681 aattacaaac tgcgttgaca gttgttgtaa ggagaccctg tatggctatg tatcagaaca
    13741 tgctcgttgt tatcgatcct aaccaggacg accaaccagc attgcggcga gctgtttatt
    13801 tacatcaacg gattggtggc aaaattaaag cctttttgcc gatctatgac ttctcatacg
    13861 aaatgaccac cctgctctcc ccggacgaac gtaccgctat gcgtcagggc gtcatcagcc
    13921 agcgtacagc ctggatccac gagcaggcaa aatattatct caatgctggc gttcccattg
    13981 aaattaaagt ggtctggcat aaccgtcctt tcgaagccat cattcaggaa gtgatcagcg
    14041 gcgggcacga tttggtgcta aaaatggcgc accaacatga ccgtctggaa gcggtgattt
    14101 ttacgccaac ggactggcat ctgttacgca aatgcccaag cccggtgtgg atggtgaaag
    14161 accagccgtg gccggaagga ggtaaggcgc tggtggcggt gaatctcgcc agtgaagagc
    14221 cgtatcataa tgcgctcaat gaaaaactgg tcaaagagac gatcgaactg gcagaacaag
    14281 tcaaccatac cgaagttcat ctggttggcg cttacccagt aacgccaatt aatatcgcga
    14341 ttgaactgcc ggaatttgag ccgagcgttt ataacgatgc cattcgtggg caacatttgc
    14401 tggcaatgaa agccctgcgg cagaaattcg gcattaatga aaacatgacg cacgtagaaa
    14461 aaggtctgcc cgaagaggtt attcctgatt tggcggagca cttacaggcg gggattgtgg
    14521 ttctgggcac ggtaggacgc accggtattt cagcagcatt cctcggcaac acggcggaac
    14581 aggtgattga tcatcttcgc tgcgatctgt tggtcattaa acctgaccag tatcagacac
    14641 ccgttgaact ggatgacgaa gaagacgatt aacgactctt caataactga caaggcccga
    14701 gttatcgggc ctatttttta cttactgctc ttcgccgcca aggaaataaa ttcccaacgg
    14761 gattgccagc aacacagtaa agaccaggct ataaacaaaa atcatcgatg actgaatcac
    14821 atacattgat gtcgtccact cagacagcgg aatattgtac tgttctataa cgccgccgat
    14881 a
//
LOCUS       DAJTYD010000089        14621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTYD010000089 DAJTYD010000000
VERSION     DAJTYD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.089"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            131..379
                     /locus_tag="OO585_002437"
     CDS             131..379
                     /locus_tag="OO585_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR6629996.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            357..1487
                     /locus_tag="OO585_002438"
     CDS             357..1487
                     /locus_tag="OO585_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6629997.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRRLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO585_002439"
     CDS             complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO585_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6629998.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2411..2500
                     /locus_tag="OO585_002440"
                     /pseudo
     CDS             2411..2500
                     /locus_tag="OO585_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2528..3271
                     /locus_tag="OO585_002441"
     CDS             2528..3271
                     /locus_tag="OO585_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6629999.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDSVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            3301..3840
                     /locus_tag="OO585_002442"
     CDS             3301..3840
                     /locus_tag="OO585_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6630000.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3945..4343
                     /gene="yciA"
                     /locus_tag="OO585_002443"
     CDS             3945..4343
                     /gene="yciA"
                     /locus_tag="OO585_002443"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6630001.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO585_002444"
     CDS             complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO585_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6630002.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            5326..5622
                     /locus_tag="OO585_002445"
     CDS             5326..5622
                     /locus_tag="OO585_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6630003.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5922..7175
                     /gene="kch"
                     /locus_tag="OO585_002446"
     CDS             5922..7175
                     /gene="kch"
                     /locus_tag="OO585_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6630004.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7230..7403)
                     /locus_tag="OO585_002447"
     CDS             complement(7230..7403)
                     /locus_tag="OO585_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6630005.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7546..9006
                     /gene="cls"
                     /locus_tag="OO585_002448"
     CDS             7546..9006
                     /gene="cls"
                     /locus_tag="OO585_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6630006.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9041..9370
                     /locus_tag="OO585_002449"
     CDS             9041..9370
                     /locus_tag="OO585_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6630007.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO585_002450"
     CDS             complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO585_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6630008.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO585_002451"
     CDS             complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO585_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6630009.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTTVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO585_002452"
     CDS             complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO585_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6630010.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO585_002453"
     CDS             complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO585_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6630011.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13378..>14621)
                     /locus_tag="OO585_002454"
     CDS             complement(13378..>14621)
                     /locus_tag="OO585_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6630012.1"
                     /translation="TASPYASYLQYGHIAGIDEILEGKKPITDLGVKAIDDHTLEVTL
                     SEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYTLKDWVVNERIVLER
                     SPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPIELFQKLKKEIPDEV
                     HVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKAQGDMPAYGYTPPYT
                     DGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYNTSDLHKKLAIAASS
                     LWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPTSFLNTMLSNSSMNT
                     AHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPVYYYVNARLVKPWVG
                     GYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3758 a         3559 c         3478 g         3826 t
ORIGIN      
        1 cgttaccagg caaaatacat ttcagttatc tggcaaaaac tgatcccgaa tcaggctgat
       61 ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc atgattagcg
      121 aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg aattcagtga
      181 accggtccct actccgagta cgttaagtaa atacgctaaa gccggaatga tatttcctct
      241 ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg tcggaatggt
      301 aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac tggaagatgg
      361 cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt gacaaaagaa
      421 ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac ggtctcggca
      481 gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt cttgcccggc
      541 agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc ggcggttcat
      601 caactgttac catttttctg aataattaca gaaaaattca acaggaaaga tatgaaaacg
      661 gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta tttgatgaac
      721 gttttggcac cagacggtta gatgccataa ccgtaaaaga tgtggtatca gtactggaag
      781 agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta ctgatcgatg
      841 ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct gcagaatcgg
      901 caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag tggatgatga
      961 tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg ctggcactga
     1021 tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc cgggatggtt
     1081 atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg gctctgcgtt
     1141 gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat tgcgttctta
     1201 gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc gggatggtta
     1261 agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat tacaactggc
     1321 gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag cgattgttca
     1381 gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa atgaccgata
     1441 tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga ccagttttgc
     1501 agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc ggagaacata
     1561 aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga tttgtactta
     1621 gccgtggtat cgatatccat gtaccagact gacatgttaa ccaaccagtc acggttaatc
     1681 agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact gagatcggaa
     1741 agccctgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt gtagttaata
     1801 cctgccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca ctgcgccatc
     1861 agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt gccgatttta
     1921 tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc catataagta
     1981 aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact tcctaacgta
     2041 ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa aaattcgcct
     2101 gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc cgccactgtt
     2161 aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata tacctatatt
     2221 ggagtaacaa atgatcctac atagatcaca ttacatatga taattcaccc tttattgatc
     2281 tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat caatttttaa
     2341 atattttcgc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc ccacgcctga
     2401 aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac ctgatgctgc
     2461 cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata aaactcaagg
     2521 agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt tcttccgtaa
     2581 tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag tggtgttagg
     2641 gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacagcg tgcccgttag
     2701 cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc aacaaatttt
     2761 gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca ttctcgccgg
     2821 aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg cgctgcgagc
     2881 tattggtgcc agtgcaccga tattgccaaa gttatttatt ctgatttttc tgactaccct
     2941 tttagtacag attggcatca tgctagtggt cgttccggga attatcatgg ccattttact
     3001 ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct cgatgcgtag
     3061 cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac tgagctggtt
     3121 gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa ccccggaaat
     3181 tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccgtattgc tcatctatct
     3241 gttccgtctg tatatgttga ttcgccaata accttaagca tttgattacg gaatcgtaaa
     3301 atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta caagatttat
     3361 gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct tatctatagc
     3421 tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct ggtggtcgtc
     3481 ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa ggttacagtc
     3541 atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa aaagccgcta
     3601 attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc gaagctgaat
     3661 ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat cgcattctgg
     3721 ctgccgcaaa atatttgggt caactttaaa gtctttggac tgaccgccct taccttaatc
     3781 ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga taaatcctaa
     3841 ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc aggaaaatca
     3901 tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct acaacacata
     3961 acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat accaatgcca
     4021 atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc gctattctgg
     4081 cgaaagaaat tgcccacggt cgcgtagtga ccgtgcgggt tgaaggaatg actttcttac
     4141 ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag aaagggacga
     4201 catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa ccaatcgggc
     4261 aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct gaaggaaaac
     4321 ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt ttgactttct
     4381 gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac aatcccactg
     4441 cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc acgctcaaac
     4501 atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc cggcgtgacg
     4561 tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc cggatactgc
     4621 ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg cttgctggtt
     4681 gcagccgttg ctgtacttga tgtcaggcgt gccagtgccg tattttcaaa cggtgatgcc
     4741 ggacgcgact ctacgggttt gacatcgcgt tttggctgct cctgtacctt tttcaccggc
     4801 ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc ttcttttggc
     4861 ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg tggcggctga
     4921 acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat tggctgcgca
     4981 ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc cacaacagca
     5041 ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa atcaagggtc
     5101 attgaagtca taatcatttc agtagaaaaa ccaagcctcg attttaaatg caaatagcaa
     5161 tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg ccgtaattaa
     5221 ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat cttcgccctt
     5281 tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt gtatgttatt
     5341 tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc ggcacattta
     5401 gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc aatgccagcg
     5461 gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat tgctgaattt
     5521 gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc ggcaggagtc
     5581 tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat tcaatattta
     5641 agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg gtcagtgcat
     5701 tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt gagcatattc
     5761 aggaaaaatg acctttcctg aggtacagtt gctcgctcaa aacctttgat tactgcggat
     5821 aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag aaaatgtgca
     5881 cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac tgggctacat
     5941 tcaaacaaac agcaacaaat ttatgggtga ccctacggca cgacattctc gcgctggccg
     6001 tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc aatttgctag
     6061 atattttcca tatcaaagca ttttcagagt tggatctttc cttgctggca aacgccccac
     6121 tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc cgggcaaagc
     6181 tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttatacc ctgcattttt
     6241 atccctggtt gaaatttagt attggatttt gcatttttac gctggtgttt ttgctgatac
     6301 tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca tttattagtt
     6361 tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa ggttttaatc
     6421 cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg tcaaccgtcg
     6481 gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt tcggtcatta
     6541 tttccggtat taccgttttt gccacatcta tgacctcaat ttttggtccg cttatccgcg
     6601 ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa gatcatttta
     6661 ttgtttgcgg acattcgatt ctcgccatca atacgattct gcaactgaat caacgcggac
     6721 aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt gagcaacgct
     6781 taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta ttaaagaaag
     6841 cgggaatcga tcgttgcctg gccattctgg cgctgagtga taacgatgca gataacgcgt
     6901 ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc gccgtcagtg
     6961 atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc tcaccgcaac
     7021 tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat aatgatatgc
     7081 tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat gaacaagaaa
     7141 cgaaagctga cagtaaagaa tcggcgcaaa aatagagttt aaagatggag gagtaaactt
     7201 attgggcacc gcaatggtgc ccagtacatt tagatattgt agatcgcaaa caataacgag
     7261 ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt tcggcgcgat
     7321 tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg ccagcgcccc
     7381 acttccgttc tactccgctt catgttaact actctatgca ataacagaat ggtcattata
     7441 cccccgcccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag atgaatccat
     7501 atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac aaccgtttat
     7561 acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg cgtaacttta
     7621 cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt gattatttac
     7681 attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca tttaggcaaa
     7741 cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct taacgacctt
     7801 aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc attattcaag
     7861 ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca actgatgacc
     7921 gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg ccataatatt
     7981 gagattgtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc tgaatcatta
     8041 atggccgctg cgcgacgcgg cattcattgc cgattgatgc tcgactccgc cgggagtgtg
     8101 gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga agtggtcgaa
     8161 gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg ccaacatcgc
     8221 aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat ggtcgatcct
     8281 cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc gcgtatggaa
     8341 ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat tgaaaccgga
     8401 aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca ggccagcggt
     8461 cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat tcaccaggca
     8521 ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgaccacgcc ctactttgtg
     8581 ccaagcgatg atttacttca tgcgatttgc acggcggcac agcgcggggt ggatgtcagt
     8641 attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg cgcattcttt
     8701 acggaacttc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt actgcatacc
     8761 aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct tgatatgcgt
     8821 agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt tggtgcagac
     8881 cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc ccgtttatgg
     8941 ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt cagcccgttg
     9001 ctgtaaaacg tctccaacag aggttaaaca ggtagtgact atggatatgg atctaaacaa
     9061 tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg aactggctgc
     9121 cgacaatctc gacccagctg atgttctgct gttcaatctt cagtttgaag agcgcggcgg
     9181 cgcggagtta ttcgatccgg tggaagactg gcaggaacat gttgatttcg atttgaaccc
     9241 ggactttttt gccgaagtgg tgattggtct ggcggacagt gaggatggtg aaatcaacga
     9301 tgttttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc atatcatctg
     9361 gcgggaataa ataatcgctt tcggcgtgaa aaagggctga caactgtcag cccttattgt
     9421 gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc ttccagcaca
     9481 ggacgtgttt tggcgcactc cggaccggca atcgggcaac gggtacggaa aacacaaccg
     9541 gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt tttgttcttc
     9601 tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta aggatgtagt
     9661 ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag atacatcacc
     9721 aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcaataaa aattaatgac
     9781 aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg aattgacacg
     9841 tccagcgccg acaccggctc atcacagata atcagcttcg gttcaagaat aagagcacga
     9901 gcaatcccga tacgctggca ctgcccacca gagaactcat gcggatagcg gttaatcagg
     9961 ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac ttcctgacgt
    10021 gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat ggtcatgcgc
    10081 gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg aacggcacgc
    10141 cattcatcgg gcttaatgcc cagcaactct ttacctaacc aggcaacatg accgtcggtc
    10201 gctttaacca ggccaatgat ggcgcgagca aaggtggact taccgcatcc cgattcccct
    10261 accacaccta atgtttcccc ttcatacagg cgaagcgtga caccatcgac ggctttgagc
    10321 gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg cactttcaga
    10381 tcggcgattt caaggaggac ttttcttcct tcggttacag cattcataac agttcctcca
    10441 ccggtttaaa gcaagcacac agacggccaa gcgtaaactc ttccagcggc ggtgcgctac
    10501 tacaaatttc catcgcatgc ggacaacgtg gctggaacgg gcaacctttc ggtaatcgca
    10561 gcaggtttgg cggattaccc gggatggtca acattgtttc accttccgca tcgagacgcg
    10621 gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag acatcgcgcg
    10681 cgttgccata ttccatcgtg cgcccggcgt acattaccag cactttgtca cagatccccg
    10741 ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt attaaattcc cgtttcagtt
    10801 catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgctgta gtgggttcat
    10861 ccgcaatcag cagcttaggt cgacatagca atgccatcgc aatcataact cgctgacgca
    10921 tgccgccaga aaactcgtgc gggtacattt tcatgcgttt acgtgcttcc ggcattttta
    10981 ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg ttcttatgca
    11041 gcatcagcac ttccatcaac tgctcaccga cgcgcatata gggattcaac gaagtcattg
    11101 ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca tgctctggca
    11161 aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg ttggcagcca
    11221 acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca cctacaatac
    11281 ccagcgtttc tccggcacgt agggaaaaat tcaaatcatt gaccgtcgtg acgtcgccgt
    11341 ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtgcgtca gcctgttgtt
    11401 gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt aacgatcttt
    11461 cgggtcgagg gcatcacgca agccatcgcc gataaagttg aaacaaaaca gcgtcaccac
    11521 gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt tcgcgccatc
    11581 actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca ggaagctaag
    11641 gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca ccaccacacc
    11701 gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac cgccaacttg
    11761 tgccgcctca ataaactctt tgcgcttcag actcagggtt tgcccacgca caatacgagc
    11821 catatccagc caggaaacca tgcctatcgc cacgaaaatc agcaggatgt tttgaccgaa
    11881 aaaggtcacc agcaaaatga cgaagaacat gaatgggaag gagttgagga tttccagcag
    11941 acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac cataaagtgt
    12001 cccgacgacc acagccacca gtgccgcagc aacaccgacc atgagtgaga tacgcccacc
    12061 aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt agtgaccgga
    12121 ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg caaactggga
    12181 aagcatcggt gccaggatta caaataacgc gatcagcacc agcaccatca gactggcaac
    12241 cgccgcacgg ttatgaataa aacgtcgacg tgcgtcctgc cacaagctgc gcccttcgac
    12301 ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta acatcatcgc
    12361 gagctccagt atcagtaacg gattttcggg tcgataaccg catatagcac atcgacaatg
    12421 gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac taaggaatag
    12481 tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc ataaatggtt
    12541 tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata agagagcacg
    12601 ggtaacagag caggttttaa tgcgtgacgt aaaatgatcc gccgcatagg taaccctttc
    12661 gcccgggcag tacgaataaa attggagtgt aatacttcaa tcatagagcc acgggtaata
    12721 cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat catgaattta
    12781 agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc aaatatcatg
    12841 actaataatg gtgcaaccac aaaactgggg ataacaaccc cggtcattgc cagccccatc
    12901 acggtatagt cccatttggt gttttgtttt aatgcggcaa taacgccagc actaacaccc
    12961 agtattaccg caaggaaaaa tgctgcggct cccagtttgg cagaaacggg aaaactggat
    13021 gcaaccaagt cattgaccga ataatcttta tatttaaacg atggaccgaa atcaccgtgc
    13081 gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag atgatatttc
    13141 gcttcgatat tggccatcac ttctggcggt aaagtacgtt cgccggtaaa agggcttccc
    13201 ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa taaatagcgt cggaatcgct
    13261 tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct gtgccttctg
    13321 taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt attgccatta
    13381 gtgcttcaca atgtacatat tccgggtata ggtattatcc agcggatctt tgccggtata
    13441 gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag gaacaatggc
    13501 cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgct gcgcctcgtc
    13561 agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat aatgcgcggt
    13621 attcatcgag ctgttcgaaa gcatggtgtt caggaaggaa gttggttcgt tgtagtcagc
    13681 acaccagcct gcacgggcca catcaaaagt accctggtga cgggtgtcga ggaacgtttt
    13741 ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca atgaagaggc
    13801 agcaatcgcc agctttttat gcagatcgga ggtgttatac aacaggttga ttgtcaacgg
    13861 tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt tacgtttttc
    13921 ctggctccag ccaaaccatt ccggctgagt caattttgcg ccatcagtat acggtggagt
    13981 gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga tatcgcggtc
    14041 catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt tctggttgtt
    14101 aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg ggatctcttt
    14161 tttcagcttc tggaacaatt cgatcggcat gttgttataa gtcatgtcga tttcaccact
    14221 acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta cctggttaat
    14281 aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc gttcgtttac
    14341 gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct gggtccattt
    14401 ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt gaacaagtaa
    14461 tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat catcaatagc
    14521 tttcacaccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa taccggcgat
    14581 atgcccatat tgcagataac tggcatacgg agaagcagtg t
//
LOCUS       DAJTYD010000090        14611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTYD010000090 DAJTYD010000000
VERSION     DAJTYD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.090"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..985)
                     /locus_tag="OO585_002455"
     CDS             complement(<1..985)
                     /locus_tag="OO585_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HCR6630013.1"
                     /translation="MSQHQYYPQLKWKPAEYESLMLLDQTTLSGFTPIITIPDIDWDY
                     ENECYKKSLSSYLSDFGINLAASWKANRPVLLDVKYLDKHGSSRHHPLDMCIQDARVN
                     GKEIIPVVSPAYSTNYIHAVQRNLINGLAISITPQTWHQFTSLVNHLNIHPSLIDVII
                     DFGDIQNATDSLKQQALSMVNTLSGQAPWRNLILSSTAYPASQAGIPQHQVHHIPRHE
                     YDLWMYVVQNFSNGRTPSFSDYPTASSTITSVDPRFMSQYVSVRYSNDTSWIFVKGTA
                     VKGNGWGQTKNLCTTLVSSPEYQVFGSKFSWGDDYIYQRSLGANKSGGSKEW"
     gene            1398..1943
                     /locus_tag="OO585_002456"
     CDS             1398..1943
                     /locus_tag="OO585_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6630014.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1918..3843
                     /locus_tag="OO585_002457"
     CDS             1918..3843
                     /locus_tag="OO585_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6630015.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3840..4046
                     /locus_tag="OO585_002458"
     CDS             3840..4046
                     /locus_tag="OO585_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6630016.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4043..5644
                     /locus_tag="OO585_002459"
     CDS             4043..5644
                     /locus_tag="OO585_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6630017.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5625..6944
                     /locus_tag="OO585_002460"
     CDS             5625..6944
                     /locus_tag="OO585_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6630018.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6954..7286
                     /locus_tag="OO585_002461"
     CDS             6954..7286
                     /locus_tag="OO585_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6630019.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDSAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            7342..8367
                     /locus_tag="OO585_002462"
     CDS             7342..8367
                     /locus_tag="OO585_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6630020.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGSTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8409..8804
                     /locus_tag="OO585_002463"
     CDS             8409..8804
                     /locus_tag="OO585_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6630021.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQTDTVTDAAALVTVVALVTLHTDALHATRDEP
                     VAFVLPETAFRVSAGVAAEMTERGLARMQ"
     gene            8816..9169
                     /locus_tag="OO585_002464"
     CDS             8816..9169
                     /locus_tag="OO585_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6630022.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9181..9759
                     /locus_tag="OO585_002465"
     CDS             9181..9759
                     /locus_tag="OO585_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6630023.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9756..10151
                     /locus_tag="OO585_002466"
     CDS             9756..10151
                     /locus_tag="OO585_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6630024.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            10159..10899
                     /locus_tag="OO585_002467"
     CDS             10159..10899
                     /locus_tag="OO585_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6630025.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDKDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            10915..11337
                     /locus_tag="OO585_002468"
     CDS             10915..11337
                     /locus_tag="OO585_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6630026.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            11319..11753
                     /locus_tag="OO585_002469"
     CDS             11319..11753
                     /locus_tag="OO585_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6630027.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSIHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11746..14307
                     /locus_tag="OO585_002470"
     CDS             11746..14307
                     /locus_tag="OO585_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6630028.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKVGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SQLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQCRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14304..>14611
                     /locus_tag="OO585_002471"
     CDS             14304..>14611
                     /locus_tag="OO585_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6630029.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     "
BASE COUNT         3431 a         3642 c         4459 g         3079 t
ORIGIN      
        1 gccattcttt agagccgcca gatttgttag cgcctaatga tctttggtaa atgtaatcat
       61 ccccccaact aaatttggaa ccaaagactt gatactctgg cgaactaaca agggtagtac
      121 ataagttttt agtttggccc catccatttc ctttaactgc ggtacctttt acaaagatcc
      181 atgaggtatc gttcgaatat ctcactgaga catactgaga catgaagcgt gggtctacgc
      241 tcgtaatggt agagctagcg gtgggataat cactaaaact tggcgttctt ccattgctaa
      301 aattctgtac tacgtacatc caaagatcgt attcatggcg cggaatatga tgaacttgat
      361 gttgcggtat ccctgcctgt gatgctgggt atgcggttga agataaaatc aagtttctcc
      421 acggagcttg gcctgataat gtgttgacca tgcttaatgc ttgttgtttt aaactatcag
      481 ttgcgttttg aatatctcca aaatcaatga ttacatcaat taaactagga tgaatattta
      541 agtggttaac cagacttgtg aattggtgcc atgtctgggg ggtgatagat atagcgagcc
      601 cattgattaa gttgcgttga acagcatgta tatagtttgt tgaatatgcg ggagaaacaa
      661 cagggataat ttccttacca tttactctag catcttggat acacatatct agaggatgat
      721 ggcggctcga accatgttta tctaaatatt taacatccag caaaacagga cgattggctt
      781 tccaggatgc cgcaaggtta ataccgaagt cagataagta agaactcaaa ctcttcttgt
      841 agcattcgtt ttcataatcc cagtctatgt ctggaatggt aatgatcgga gtaaaaccag
      901 agagcgtagt ttgatctaaa agcatcagag attcatattc agcaggcttc catttcagct
      961 gtggataata ttgatgttga ctcattaaaa ctccttaaaa tctttactta tcctaagtat
     1021 agtgttatgg cgtaacgcca attgttttta tacacttgaa aacattggga aaagttgagc
     1081 tatatctaaa tttacaaaat tggatgttat agaaatcgag gtttcaacta ggcatggaac
     1141 tgcaaaattt ttaatgcctg cacgcgaaag tcgttggcgg gtcctttccg gtgatccaga
     1201 ccgttacggg gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg
     1261 cgtttccgtt cttcttcgtc gtaacttaat gtttttattt aaaatacccc ctgaaaagaa
     1321 aggaaacgac aggtgctgaa aacgaacttt tgggcctttg tcgtttcctt tctctgtttt
     1381 tggccgtgga atgaacaatg gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga
     1441 gtatccgtac cattcagaac tggcaggaac agggaatgcc cgttctgcga ggcggtggca
     1501 agggtaatga ggtgctttat gactctgccg ccgttataaa atggtatgcc gaaagggatg
     1561 ctgaaattga gaacgaaaag ctgcgccggg aggttgaaga actgcgccag gccagcgagg
     1621 cagatctcca gccagggact attgagtacg aacgccatcg acttacgcgt gcgcaggccg
     1681 acgcacagga actgaagaat gccagagact cagctgaagt ggtggaaacc gcattctgta
     1741 ctttcgtgct gtcgcggatc gcaggtgaaa ttgccagtat tctcgacggg atccccctgt
     1801 cggtgcagcg gcgttttccg gaactggaaa accgacatgt tgatttcctg aaacgggata
     1861 tcatcaaagc catgaacaaa gcagccgcgc tggatgaact gataccgggg ttgctgagtg
     1921 aatatatcga acagtcaggt taacaggctg cggcattttg tccgcgccgg gcttcgctca
     1981 ctgttcaggc cggagccaca gaccgccgtt gaatgggcgg atgctaatta ctatctcccg
     2041 aaagaatccg cataccagga agggcgctgg gaaacactgc cctttcagcg ggccatcatg
     2101 aatgcgatgg gcagcgacta catccgtgag gtgaatgtgg tgaagtctgc ccgtgtcggt
     2161 tattccaaaa tgctgctggg tgtttatgcc tactttatag agcataagca gcgcaacacc
     2221 cttatctggt tgccgacgga tggtgatgcc gagaacttta tgaaaaccca cgttgagccg
     2281 actattcgtg atattccgtc gctgctggcg ctggccccgt ggtatggcaa aaagcaccgg
     2341 gataacacgc tcaccatgaa gcgtttcact aatgggcgtg gcttctggtg cctgggcggt
     2401 aaagcggcaa aaaactaccg tgaaaagtcg gtggatgtgg cgggttatga tgaacttgct
     2461 gcctttgatg aggatattga acaggaaggc tctccgacgt tccttggcga caaacgtatt
     2521 gaaggctcgg tctggccaaa gtccatccgt ggctccacgc ccaaagtgag aggcacctgc
     2581 cagattgagc gtgcagccag tgaatccccg cattttatgc gttttcatgt tgcctgcccg
     2641 cactgcgggg aggagcagta tcttaaattt ggcgacaaag agacgccgtt tggcctcaaa
     2701 tggacgccgg atgacccctc cagcgtgttt tatctctgcg agcataatgc ctgcgtcatc
     2761 cgccagcagg agctggactt tactgatgcc cgttatatct gcgaaaagac cgggatctgg
     2821 acccgtgatg gcattctctg gttttcgtca tccggtgaag agattgagcc acctgacagt
     2881 gtgacctttc acatctggac agcgtacagc ccgttcacca cctgggtgca gattgtcaaa
     2941 gactggatga aaacgaaagg ggatacggga aaacgtaaaa ccttcgtaaa caccacgctc
     3001 ggtgagacgt gggaggcgaa aattggcgaa cgtccggatg ctgaagtgat ggcagagcgg
     3061 aaagagcatt attcagcgcc cgttcctgac cgtgtggctt acctgaccgc cggtatcgac
     3121 tcccagctgg accgctacga aatgcgcgta tggggatggg ggccgggtga ggaaagctgg
     3181 ctgattgacc ggcagattat tatgggccgc cacgacgatg aacagacgct gctgcgtgtg
     3241 gatgaggcca tcaataaaac ctatacccgc cggaatggtg cagaaatgtc gatatcccgt
     3301 atctgctggg atactggcgg gattgacccg accattgtgt atgaacgctc gaaaaaacat
     3361 gggctgttcc gggtgatccc cattaaaggg gcatccgtct acggaaagcc ggtggccagc
     3421 atgccacgta agcgaaacaa aaacggggtt taccttaccg aaatcggtac ggataccgcg
     3481 aaagagcaga tttataaccg cttcacactg acgccggaag gggatgaacc gcttcccggt
     3541 gccgttcact tcccgaataa cccggatatt tttgatctga ccgaagcgca gcagctgact
     3601 gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa
     3661 aagcgacgca atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt
     3721 atttcccgct ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt
     3781 gcagcaacca acaagaaaac actggcagat tacgcccgtg ccttatccgg agaggatgaa
     3841 tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa
     3901 aacgggtggc aacggtacag aaagacggac ggagagtgga gtttacggcc acttccgtgt
     3961 ctgacctgaa aaaatacatt gcggagctgg aagtgcagac cggcatgaca cagcgacgca
     4021 ggggacctgc aggattttat gtatgaaaac gtccaccatt cccacccttc tggggccgga
     4081 cggcatgaca tcgctgcgtg aatatgccgg ttatcacggc ggtggcagcg gatttggagg
     4141 gcagttgcgg gcgtggaacc caccgagtga aagtgtggat gcagccctgc tgcccaactt
     4201 tacccgtggc aatgcccgcg cagacgatct ggtacgcaat aacggctatg ccgccaacgc
     4261 catccagttg catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag
     4321 ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc
     4381 ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac
     4441 gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt
     4501 tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag
     4561 cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt
     4621 gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg
     4681 ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcctcgtt
     4741 cattcacgtt tttgaacccg tggaggacgg gcagactcgc ggtgcaaatg tgttttacag
     4801 cgtgatggag cagatgaaga tgctcgacac gctgcagaac acgcagctgc agagcgccat
     4861 tgtgaaggcg atgtatgccg ccaccattga gagtgagctg gatacgcagt cagcgatgga
     4921 ttttattctg ggcgcgaaca gtcaggagca gcgggaaagg ctgaccggct ggattggtga
     4981 aattgccgcg tattacgccg cagcgccggt ccggctggga ggcgcaaaag taccgcacct
     5041 gatgccgggt gactcactga acctgcagac ggctcaggat acggataacg gctactccgt
     5101 gtttgagcag tcactgctgc ggtatatcgc tgccgggctg ggtgtctcgt atgagcagct
     5161 ttcccggaat tacgcccaga tgagttactc cacggcacgg gccagtgcga acgagtcgtg
     5221 ggcgtacttt atggggcggc gaaaattcgt cgcatcccgt caggcgagcc agatgtttct
     5281 gtgctggctg gaagaggcca tcgttcgccg cgtggtgacg ttaccttcaa aagcgcgctt
     5341 cagttttcag gaagcccgca gtgcctgggg gaactgcgac tggataggct ccggtcgtat
     5401 ggccatcgat ggtctgaaag aagttcagga agcggtgatg ctgatagaag ccggactgag
     5461 cacctacgag aaagagtgcg cgaaacgcgg tgacgactat caggaaattt ttgcccagca
     5521 ggtccgtgaa acgatggagc gccgtgcagc cggtcttaaa ccgcccgcct gggcggctgc
     5581 agcatttgaa tccgggctgc gacaatcaac agaggaggag aagagtgaca gcagagctgc
     5641 gtaatctccc gcatattgcc agcatggcct ttaatgagcc gctgatgctt gaacccgcct
     5701 atgcgcgggt tttcttttgt gcgcttgcag gccagcttgg gatcagccgc ctgacggatg
     5761 cggtgtccgg cgacagcctg actgcccagg aggcactcgc gacgctggca ttatccggtg
     5821 atgatgacgg accacgacag gcccgcagtt atcaggtcat gaacggcatc gccgtgctgc
     5881 cggtgtccgg cacgctggtc agccggacgc gggcgctgca gccgtactcg gggatgaccg
     5941 gttacaacgg cattatcgcc cgtctgcaac aggctgccag cgatccgatg gtggacggca
     6001 ttctgctcga tatggacacg cccggcggga tggtggcggg ggcatttgac tgcgctgaca
     6061 tcatcgcccg tgtgcgtgac ataaaaccgg tatgggcgct tgccaacgac atgaactgca
     6121 gtgcaggtca gttgcttgcc agtgccgcct cccggcgtct ggtcacgcag accgcccgga
     6181 caggctccat cggcgtcatg atggctcaca gtaattacgg tgctgcgctg gagaaacagg
     6241 gtgtggaaat cacgctgatt tacagcggca gccataaggt ggatggcaac ccctacagcc
     6301 atcttccgga tgacgtccgg gagacactgc agtcccggat ggacgcaacc cgccagatgt
     6361 ttgcgcagaa ggtgtcggca tataccggcc tgtccgtgca ggctgtgctg gataccgagg
     6421 ctgcagtgta cagcggtcag gaggccattg atgccggact ggctgatgaa cttgttaaca
     6481 gcaccgatgc gatcaccgtc atgcgtgatg cactggatgc acgtaaatcc cgtctctcag
     6541 gagggcgaat gaccaaagag actcaatcaa caactgtttc agccactgct tcgcaggctg
     6601 acgttactga cgtggtgcca gcgacggagg gcgagaacgc cagcgcggcg cagccggacg
     6661 tgaacgcgca gatcaccgca gcggttgcgg cagaaaacag ccgcattatg gggatcctca
     6721 actgtgagga ggctcacgga cgcgaagaac aggcacgcgt gctggcagaa acccccggta
     6781 tgaccgtgaa aacggcccgc cgcattctgg ccgcagcacc acagagtgca caggcgcgca
     6841 gtgacactgc gctggatcgt ctgatgcagg gggcaccggc accgctggct gcaggtaacc
     6901 cggcatctga tgccgttaac gatttgctga acacaccagt gtaagggatg tttatgacga
     6961 gcaaagaaac ctttacccat taccagccgc agggcaacag tgacccggct cataccgcaa
     7021 ccgcgcccgg cggattgagt gcgaaagcgc ctgcaatgac cccgctgatg ctggacacct
     7081 ccagccgtaa gctggttgcg tgggatggca ccaccgacag tgctgccgtt ggcattcttg
     7141 cggttgctgc tgaccagacc agcaccacgc tgacgttcta caagtccggc acgttccgtt
     7201 atgaggatgt gctctggccg gaggctgcca gcgacgagac gaaaaaacgg accgcgtttg
     7261 ccggaacggc aatcagcatc gtttaacttt acccttcatc actaaaggcc gcctgtgcgg
     7321 ctttttttac gggatttttt tatgtcgatg tacacaaccg cccaactgct ggcggcaaat
     7381 gagcagaaat ttaagtttga tccgctgttt ctgcgtctct ttttccgtga gagctatccc
     7441 ttcaccacgg agaaagtcta tctctcacaa attccgggac tggtaaacat ggcgctgtac
     7501 gtttcgccga ttgtttccgg tgaggttatc cgttcccgtg gcggctccac ctctgaattt
     7561 acgccgggat atgtcaagcc gaagcatgaa gtgaatccgc agatgaccct gcgtcgcctg
     7621 ccggatgaag atccgcagaa tctggcggac ccggcttacc gccgccgtcg catcatcatg
     7681 cagaacatgc gtgacgaaga gctggccatt gctcaggtcg aagagatgca ggccgtttct
     7741 gccgtgctca agggcaaata caccatgacc ggtgaagcct tcgatccggt tgaggtggat
     7801 atgggccgca gtgcggcgaa caacatcacg cagtccggca gcacggagtg gagcaagcgt
     7861 gacaagtcca cgtatgaccc gaccgacgat atcgaagcct acgcgctgaa cgccagcggc
     7921 gtggtgaata tcatcgtgtt tgatccgaaa ggctgggcgc tgttccgttc cttcaaagcc
     7981 gtcagggaga agctggatac ccgtcgcggc tctcattccg agctggagac agcggtgaaa
     8041 gacctgggta aagcggtgtc ctataagggg atgtatggcg atgtggccat cgtcgtgtat
     8101 tccggacagt acgtggaaaa cggcgtcaaa aagaacttcc tgccggacaa cacgatggta
     8161 ctggggaaca ctcaggcacg cggtctgcgc acctatggct gcattcagga tgcggacgca
     8221 cagcgcgaag gcattaacgc ctctgcccgt tacccgaaaa actgggtgac caccggcgat
     8281 ccggcgcgtg agttcaccat gattcagtca gcaccgctga tgctgctggc tgaccctgat
     8341 gagttcgtgt ccgtacaact ggcgtaatcg tggcccttcg gggccatttt ctctctgtgg
     8401 aggagtccat gacgaaagat gaactgattg tccgtctccg gtcgctgggt gagcaactga
     8461 accgtgatgt cagcctgacg gggacgaaag aagaactggc gctccgtgtg gcagagctgg
     8521 aagaggagct tgatgacacg gatgacgctg ccggtcagga tacccctctc agaccggaaa
     8581 atgcgctgac cggacatgaa aatgaggtgg tatcagcaca gacggatacc gtgactgatg
     8641 cggctgctct ggtcacggtt gtggcactgg tgacgctgca taccgatgca cttcacgcca
     8701 cgcgggatga acctgtggca tttgtgctgc cggaaacggc gttccgtgtc tctgccggtg
     8761 tggcagctga aatgacagag cgcggcctgg ccagaatgca ataacgggag gcgctgtggc
     8821 tgatttcgat aacctgttcg atgctgccat tgcccgcgcc gatgaaacga tacgcgggta
     8881 catgggaacg tcagccacca tgacatccgg tgagcagtcc ggcgcagtaa tacgtggtgt
     8941 ttttgatgac cctgaaaata tcagctatgc cggacagggc gtacgcgttg aaggctccag
     9001 cccgtccctg tttgtccgga ctgatgaggt gcggcagctg cggcgcggcg acacgctgac
     9061 catcggtgag gaaaacttct ggatagaccg gatttcgccg gatgatggcg gaagctgtca
     9121 tctctggctt gggcggggcg taccgcctgc cgttaaccgt cgccgctgaa agggggatgt
     9181 atggccataa aaggtcttga gcaggccgtt gaaaacctca gccgtatcag taaaacggcg
     9241 gtgcccggtg ccgccgcaat ggccattaac cgcgttgctt catccgcgat atcgcagtcg
     9301 gcgtcacagg ttgctcgtga gacaaaggta cgccggaaac tggtaaagga aagggccagg
     9361 ctgaaaaggg ccacggtcaa aaatccgcag gccagaatca aagttaaccg gggggatttg
     9421 cccgtaatca agctgggtaa tgcgcgggtt gtcctttcgc gccgcaggcg tcgtaaaaag
     9481 gggcagcgtt catccctgaa aggtggcggc agcgtgcttg tggtgggaaa ccgtcgtatt
     9541 cccggcgcgt ttattcagca actgaaaaat ggccgctggc atgtcatgca gcgtgtggcc
     9601 gggaaaaacc gttaccccat tgatgtggtg aaaatcccga tggcggtgcc gctgaccacg
     9661 gcgtttaaac agaatattga acggatacgg cgtgaacgtc ttccgaaaga gctgggctat
     9721 gcgctgcagc atcaactgag aatggtaata aagcgatgaa acatactgaa ctccgtgcag
     9781 ccgtactgga tgcactggag aagcatgaca ccggggcgac gctttttgat ggtcgccccg
     9841 ctgtttttga tgaggaagat tttccggcaa ttgccgttta tctcaccggc gctgaataca
     9901 cgggcgaaga gctggacagc gatacctggc aggcggagct gcatattgaa gttttcctgc
     9961 ctgctcaggt gccggattca gagctggatg cgtggatgga gtcccggatt tatccggtga
    10021 tgagcgatat cccggcactg tcagatttga tcaccagtat ggtggccagt ggctatgact
    10081 accggcgcga cgatgatgcg ggcctgtgga gttcagccga tctgacttat gtcattacct
    10141 atgaaatgtg aggacgctat gcctgtacca aatcctacaa tgccggtgaa aggtgccggg
    10201 accaccctgt gggtttataa ggggagcggt gacccttatg cgaacccgct ttcagacgtt
    10261 gactggtcgc gtctggcaaa agttaaagac ctgacgcccg gcgaactgac cgctgagtcc
    10321 tatgacgaca gctatctcga tgataaagat gcagactgga ctgcgaccgg gcaggggcag
    10381 aaatctgccg gagataccag cttcacgctg gcgtggatgc ccggagagca ggggcagcag
    10441 gcgctgctgg cgtggtttaa tgaaggtgat acccgtgcct ataaaatccg cttcccgaac
    10501 ggcacggtcg atgtgttccg tggctgggtc agcagtatcg gtaaggcggt gacggcgaag
    10561 gaagtgatta cccgcacggt gaaggtcacc aatgtgggac gtccgtcgat ggcagaagat
    10621 cgcagcacgg taacagcggc aaccggcatg accgtgacgc ctgccagcac ttcggtggtg
    10681 aaagggcaga gcaccacgct gaccgtggca ttccagccgg aaggcgcaac cgacaagagc
    10741 ttccgtgcgg tgtctgcgga taaaacaaaa gccaccgtgt cggtcagtgg tatgaccatc
    10801 accgtgaaag gtgttgctgc aggcaaggtc aacattccgg ttgtatccgg taatggtgaa
    10861 cttgctgtgg ttgcagaaat caccgtcacc gacagttaat ccggagagtc agcgatgttc
    10921 ctgaaaaccg aatcatttga atataacggt gtgagcgtca cgctttctga actgtcagcc
    10981 ctgcagcgaa ttgagcatct cgccctgctg aaacgacagg cagaacaggc gggatccagt
    11041 ctcaatcgac aggtgagcgt ggaagatctc gtcagaaccg gtgcttttct ggtggcgatg
    11101 tccctgtggc atagccatcc gcagaagaca aagatgccgt ccatgaatga agccgttaaa
    11161 caaattgagc aggaagtgct taccacctgg cccacagagg caattgctca ggctgaaaat
    11221 gtggtaatgc gtctgtccgg tatgtctgag tttgttgtga atgatgcacc tgaacaggca
    11281 gatgacgccg ggccagcaga gcctgtttct gcgggaaagt gttcgacggt gagctgagtt
    11341 ttgccctgaa actggcgcgt gagatggggc gacccgactg gcgcgccatg cttgccggga
    11401 tgtcatccac ggagtatgcc gactggcacc gcttttacag tatccattat tttcatgatg
    11461 ttctgctgga tatgcacttt tccgggctga cgtacaccgt actcagcctg tttttcagcg
    11521 atccggatat gcatccgctg gatttcagtc tgctgaaccg gcgcgaggct gacgaagagc
    11581 ctgaagatga tgtgctgatg cagaaagcgg cagggcttgc cggaggcgtc cgctttggcc
    11641 cggacgggaa tgaagttatc cccgcttccc cggatgtggc ggacatgacg gaggatgacg
    11701 taatgctgat gacagtatca gaagggatcg caggaggagt ccggtatggc tgaaccggta
    11761 ggcgatctgg tcgttgattt gagtctggat gcggccagat ttgacgagca gatggccaga
    11821 gtcaggcgtc atttttccgg tacggaaagt gatgcgaaaa aaacagcggc agtcgttgaa
    11881 cagtcgatga accggcaggc gctggctgca cagaaagtgg gaatttccgt cgggcagtat
    11941 aaagccgcca tgcgtatgct gcctgcgcag ttcactgacg tggccacgca gcttgcaggc
    12001 gggcaaagtc cgtggctgat cctgctgcaa caggggggtc aggtgaagga ctccttcggc
    12061 gggatgatcc ccatgttccg ggggcttgcc ggtgcgatca ccctgccgat ggtgggggcc
    12121 acctcgctgg cggtggcgac cggagcgctg gcgtatgcct ggtatcaggg caactcaacc
    12181 ctgtccgatt tcaacaaaac gctggtcctt tccggcaatc aggcgggact gacggcagat
    12241 cgtatgctgg tcctgtccag agccgggcag gcggcagggc tgacgtttaa ccagaccagc
    12301 gagtcactca gcgcactggt taaggcgggg gtaagcggtg aggctcagat tgcgtccatc
    12361 agccagagtg tggcgcgttt ctcctctgca tccggcgtgg aggtggacaa ggtcgctgaa
    12421 gccttcggga agctgaccac agacccgacg tcggggctga cggcgatggc acgccagttc
    12481 cataacgtga cggcggagca gattgcgtat gttgctcagt tgcagcgttc cggcgatgaa
    12541 gccggggcat tgcaggcggc gaacgaggcc gcaacgaaag ggtttgatga ccagacccga
    12601 cgcctgaaag agaacatggg cacgctggaa acctgggcag acaggacagc acgggcattc
    12661 aaatccatgt gggatgcggt gctggatatt ggtcgtcctg ataccgctca ggagatgctg
    12721 attaaggcag aggctgcgtt taagaaagca gacgacatct ggaatctgcg caaggatgat
    12781 tattttgtta acgatgaagc gcgggcgcgt tactgggatg atcgtgaaaa ggcccgtctt
    12841 gcgcttgaag ccgcccgaaa gaaggctgag cagcagactc aacaggacaa aaatgcgcag
    12901 cagcagagcg atactgaagc gtcacagctg aaatataccg aagaggcgca gaaggcttac
    12961 gaacggctgc agacgccgct ggagaaatat accgcccgtc aggaagaact gaacaaggca
    13021 ctgaaagacg ggaaaatcct gcaggcggat tacaacacgc tgatggcggc ggcgaaaaag
    13081 gattatgaag cgacgctgaa aaagccgaaa cagtccggcg tgaaggtgtc tgcgggcgat
    13141 cgtcaggaag acagtgctca tgctgccctg ctgacgcttc aggcagaact ccggacgctg
    13201 gagaagcatg ccggagcaaa tgagaaaatc agccagcagc gccgggattt gtggaaggcg
    13261 gagagtcagt tcgcggtact ggaggaggcg gcgcaatgtc gccagctgtc tgcacaggag
    13321 aaatccctgc tggcgcataa agatgagacg ctggagtaca aacgccagct ggctgcactt
    13381 ggcgacaagg ttacgtatca ggagcgcctg aacgcgctgg cgcagcaggc ggataaattc
    13441 gcacagcagc aacgggcaaa acgggccgcc attgatgcta aaagccgggg gctgactgac
    13501 cggcaggcag aacgggaagc cacggaacag cgcctgaagg aacagtatgg cgataatccg
    13561 ctggcgctga ataacgtcat gtcagagcag aaaaagactt gggcggctga agaccagctt
    13621 cgcgggaact ggatggcagg cctgaagtcc ggctggagtg agtgggaaga gagcgccacg
    13681 gacagtatgt cgcaggtaaa aagtgcagcc acgcagacct ttgatggtat tgcacagaat
    13741 atggcggcga tgctgaccgg cagtgagcag aactggcgca gcttcacccg ttccgtgctg
    13801 tccatgatga cagaaattct gcttaagcag gcaatggtgg ggattgtcgg gagtatcggc
    13861 agcgccattg gcggggctgt tggtggcggc gcatccgcgt caggcggtac agccattcag
    13921 gccgctgcgg cgaaattcca ttttgcaacc ggaggattta cgggaaccgg cggcaaatat
    13981 gagccagcgg ggattgttca ccgtggtgag tttgtcttca cgaaggaggc aaccagccgg
    14041 attggcgtgg ggaatcttta ccggctgatg cgcggctatg ccaccggcgg ttatgtcggt
    14101 acaccgggca gcatggcaga cagccggtcg caggcgtccg ggacgtttga gcagaataac
    14161 catgtggtga ttaacaacga cggcacgaac gggcagatag gtccggctgc tctgaaggcg
    14221 gtgtatgaca tggcccgcaa gggtgcccgt gatgaaattc agacacagat gcgtgatggt
    14281 ggcctgttct ccggaggtgg acgatgaaga ccttccgctg gaaagtgaaa cccggtatgg
    14341 atgtggcttc ggtcccttct gtaagaaagg tgcgctttgg tgatggctat tctcagcgag
    14401 cgcctgccgg gctgaatgcc aacctgaaaa cgtacagcgt gacgctttct gtcccccgtg
    14461 aggaggccac ggtactggag tcgtttctgg aagagcacgg gggctggaaa tcctttctgt
    14521 ggacgccgcc ttatgagtgg cggcagataa aggtgacctg cgcaaaatgg tcgtcgcggg
    14581 tcagtatgtt gcgtgttgag ttcagcgcag a
//
LOCUS       DAJTYD010000091        14560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTYD010000091 DAJTYD010000000
VERSION     DAJTYD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.091"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO585_002472"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO585_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6630030.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO585_002473"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO585_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6630031.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO585_002474"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO585_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6630032.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO585_002475"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO585_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6630033.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO585_002476"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO585_002476"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6630034.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO585_002477"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO585_002477"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6630035.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO585_002478"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO585_002478"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6630036.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO585_002479"
     CDS             complement(4598..5320)
                     /locus_tag="OO585_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6630037.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO585_002480"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO585_002480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6630038.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO585_002481"
     CDS             6520..6738
                     /locus_tag="OO585_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6630039.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO585_002482"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO585_002482"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6630040.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO585_002483"
     CDS             complement(7472..7672)
                     /locus_tag="OO585_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6630041.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO585_002484"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO585_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6630042.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO585_002485"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO585_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6630043.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO585_002486"
     CDS             10175..12088
                     /locus_tag="OO585_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6630044.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO585_002487"
     CDS             12088..13110
                     /locus_tag="OO585_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6630045.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO585_002488"
     CDS             13104..13322
                     /locus_tag="OO585_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6630046.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO585_002489"
     CDS             13376..14245
                     /locus_tag="OO585_002489"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6630047.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..>14560)
                     /locus_tag="OO585_002490"
     CDS             complement(14300..>14560)
                     /locus_tag="OO585_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6630048.1"
                     /translation="AIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVT
                     GRDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3600 a         3970 c         3585 g         3405 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcagt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccaag tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacca catcgatggc
//
LOCUS       DAJTYD010000092        14414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTYD010000092 DAJTYD010000000
VERSION     DAJTYD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.092"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(58..134)
                     /locus_tag="OO585_002491"
     tRNA            complement(58..134)
                     /locus_tag="OO585_002491"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Arg,seq:tct)
     gene            complement(148..224)
                     /locus_tag="OO585_002492"
     tRNA            complement(148..224)
                     /locus_tag="OO585_002492"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Arg,seq:tcg)
     gene            complement(232..307)
                     /locus_tag="OO585_002493"
     tRNA            complement(232..307)
                     /locus_tag="OO585_002493"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Ile,seq:cat)
     gene            complement(357..1407)
                     /locus_tag="OO585_002494"
                     /pseudo
     CDS             complement(357..1407)
                     /locus_tag="OO585_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(1515..1755)
                     /locus_tag="OO585_002495"
                     /pseudo
     CDS             complement(1515..1755)
                     /locus_tag="OO585_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1982..2803)
                     /locus_tag="OO585_002496"
     CDS             complement(1982..2803)
                     /locus_tag="OO585_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6630049.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(2800..3180)
                     /locus_tag="OO585_002497"
     CDS             complement(2800..3180)
                     /locus_tag="OO585_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6630050.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(3181..4164)
                     /locus_tag="OO585_002498"
     CDS             complement(3181..4164)
                     /locus_tag="OO585_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6630051.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     DERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQNPPQEKTVVSI
                     AVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGYGLGGMGTKA
                     DDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(4238..4510)
                     /locus_tag="OO585_002499"
     CDS             complement(4238..4510)
                     /locus_tag="OO585_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630052.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(4678..4833)
                     /gene="hokD"
                     /locus_tag="OO585_002500"
     CDS             complement(4678..4833)
                     /gene="hokD"
                     /locus_tag="OO585_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6630053.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5071..5736)
                     /locus_tag="OO585_002501"
     CDS             complement(5071..5736)
                     /locus_tag="OO585_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6630054.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(5911..6336)
                     /locus_tag="OO585_002502"
     CDS             complement(5911..6336)
                     /locus_tag="OO585_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6630055.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(6352..7122)
                     /locus_tag="OO585_002503"
     CDS             complement(6352..7122)
                     /locus_tag="OO585_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6630056.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(7144..7886)
                     /locus_tag="OO585_002504"
                     /pseudo
     CDS             complement(7144..7886)
                     /locus_tag="OO585_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(7893..8855)
                     /locus_tag="OO585_002505"
     CDS             complement(7893..8855)
                     /locus_tag="OO585_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6630057.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(8878..9303)
                     /locus_tag="OO585_002506"
     CDS             complement(8878..9303)
                     /locus_tag="OO585_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6630058.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDPPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(9287..9610)
                     /locus_tag="OO585_002507"
     CDS             complement(9287..9610)
                     /locus_tag="OO585_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6630059.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            9735..10211
                     /locus_tag="OO585_002508"
     CDS             9735..10211
                     /locus_tag="OO585_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630060.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKKAIVAFILSGNATEPSWVDYDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            10530..10682
                     /locus_tag="OO585_002509"
     CDS             10530..10682
                     /locus_tag="OO585_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6630061.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            10694..11014
                     /locus_tag="OO585_002510"
     CDS             10694..11014
                     /locus_tag="OO585_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630062.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKLLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENYFATKSINS
                     FDSK"
     gene            complement(10992..11267)
                     /locus_tag="OO585_002511"
     CDS             complement(10992..11267)
                     /locus_tag="OO585_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630063.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLILNQKN"
     gene            11829..12017
                     /locus_tag="OO585_002512"
     CDS             11829..12017
                     /locus_tag="OO585_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCR6630064.1"
                     /translation="METLLPNINTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            12014..12205
                     /locus_tag="OO585_002513"
     CDS             12014..12205
                     /locus_tag="OO585_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR6630065.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            12298..>14414
                     /locus_tag="OO585_002514"
     CDS             12298..>14414
                     /locus_tag="OO585_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6630066.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHSENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPL
                     IAMNGHHVITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREA"
BASE COUNT         3672 a         3804 c         3332 g         3606 t
ORIGIN      
        1 ctgaattgtg agacatttaa aaaaaagccc gcaaaagcga gccagggaaa ataagtgtgg
       61 cgcgttgtac tggattcgaa ccagtgaccg attgcttaga aggcaattgc tctgtccggc
      121 tgagctaaca acgcaggata cagataatgg accgccttcg gggacccgaa ctccgcgcaa
      181 ccagcttcga aggctggcgc tctttcctga tgagctaatg gcggtatgtg atggtggccc
      241 ttgctggatt tgaaccagcg acctggcgat tatgagtcgc tcgctctcac cactgagcta
      301 aagggccggg agcagaataa taatggtgcg taattaattc tgcaatctca tccgtttcaa
      361 acgattaaat cctgaacttc cctgactgtc tgctcaaaac gtccggtctc cagttcaacg
      421 ccaatcgcac gacgcccgag cgccagtgca gcttttaccg ttgaacctga gcccataaaa
      481 aaaatctgca accaggtctc ccggacgact gcttgcgctg attatctgct gcagcatttc
      541 tgccggtttt tcgcacggat gtttcccggg atagaactgc accggtttat gtgtccacac
      601 atccgtgtac ggcacctgcg ccgtcacacc aaaataccgc cgcagatgct tatattcact
      661 ctgcagctcc acatactgcc ggttcagtga cgtatacgta tccaccagct ggtggtgggg
      721 cttttccagt tcaccgcgct gatgtttctc ttctgccacc cgggcaaaca gcgactgtaa
      781 tttcagataa tcgctttcgt tcggtagctg ccactgactg gcactgaacc agtgcgacac
      841 catgtttttc tttcctgtgg catctgcaat ctgttttgcc gttatcccca gggccgcgcg
      901 cgcatcacga aagtaagaaa tcagcggggc catcacatgc tgtttcagtg cactgccctt
      961 cgccgcatac ccggcatctt tcggacgata cggcccctga taatgttccg cgaacagaat
     1021 gcgctctgtg gcggggaaat acgcccgcag gctttccttg ttgcatccgt tccagcgtcc
     1081 ggacggcttc gcccagataa tatggttcag cacactgaag cgttcacgca tcatgatttc
     1141 gatatcagat gccaggcgat gaccacagaa caggtaaaga cttccggcag gtttcagcac
     1201 ccgccagaac tgcgccagac actggtccag ccacttcagg taatcatcgt cgcccttcca
     1261 ctggttatcc cagccctcag gcttcacttt aaagtacggc gggtccgtga ctatcaggtc
     1321 aacagaattt tcgggtaacg accggataaa ttccaggcag tcggcgttga ttaactcaca
     1381 actggatatt tttacagtat taagcatgga tcattaagcc tgtctctgat aggctcattc
     1441 tgcttttgcg caaagcagtg ggcctgaggt ttgcttgtga acccaacgca tgagcagatg
     1501 gctggtgggt gcccctaaca cccaccagcc gcccatttac cacaaataaa aaagccttca
     1561 ctgcggaagg cgtctgtaac aaccgaactg atagtctgcc agacccgcca taaccagctg
     1621 ggtcagtatt aactggcagc gttcgcgtga aaggtaagta ttctgtgcaa tctccccgac
     1681 tgtcgccggt tcggtaacgc ttaattcatt aaacaccact ctggcggttt ctgtcatatc
     1741 ctgctgtttt agcatgtctt tttccctttt ccggttaacg tgacacacca ataactcttg
     1801 tcgaaaaagc cagcaagctg aaagacaggt attcaccgcc accagcgcgt ttactgtact
     1861 gacgcgattt cagtcataaa aaacccgcca ggcggcgggg tgtaaaaaat cttctaacgt
     1921 caggcataaa acgcccatcg ttagagcaaa tttaccacag attcgggaaa aatcaacaac
     1981 actatcgcgt taccctcttt aactgccgct ccgcccatgc ctcttcaatg tcaaaccgaa
     2041 ccaccaacgt atcgtaaaag cgtttcactg attttttcca cgtatcaagc gtgatagcac
     2101 tcgtcacttt gcatatggca ttaaatgcct ccgttgatgg tagtctttca cagccacgac
     2161 caccacaacg ctggcagtct ctgataacag gcataccacg ttttaccgac tcttcacgat
     2221 gaatggcgac accacgccca cggcaatcct tacaggcggt ggaaacctca ccctttccgc
     2281 cacactccgg acaggcaact tttaccacct ccctgacttt tttccattct tcccagtaag
     2341 acggatacac acctttcgta cactttgccc ataccggcgg cttaccatcc ggatactgga
     2401 ccttgtttgt aaaaactacg ctttcaataa atttttcccc atagcaacaa gggcactgct
     2461 ttttactcgc tgcgctgcgg gcataatcct caaaagcgta cgaagccata atgcgcatca
     2521 ctaccggttt tatttctgcc gggagttttc ttaacgccgc cacgcgatca caccgactga
     2581 gtgcatattc tgtcagcaat tctgttgccc gctctctgtc attcatacta atgcccattt
     2641 tcccaaggaa cgcagaaaac cccatctcag cccgattctg tgtcatgccc tgcgcggcca
     2701 tcacatcagt gatactcagc gcatctttcg acgttgaggc cgatgcatca gtcaggccgg
     2761 gggattttgg ggagtagtat ttcggtaaat cttccagttt cattttttga cctgcccttc
     2821 aagcattatg gggtaaatct tcacccccag acgtccacca gatactggct gagcacgaac
     2881 gatattgatt tcatcaaact gctcatcgtc cattagcaac cccgcatgcg tcagcgcatc
     2941 cagcggcgct ttcagaatat tgtccaggtc acggcggcgc ttatccggtg gttctgcaat
     3001 aatttttatt gccaaccttc cggacaggct taatttcagc cgctgctggc gaacaataag
     3061 cgccactgcc cggcgataac gctccccggc ttttgataca aaatatgtgc tgccacgacg
     3121 acgccagtaa gtgttcaccg ttggcgggta aggcaaaaca aattctatgc gttcagtcat
     3181 ttatgctttc cacttcagaa cacccgaatt tctcgcgtgc attaaaaaac gaatcagcaa
     3241 caacagctgg ctgccgtgtt tttcttcaaa atcttttacc ccggcgtgta gttcgctatg
     3301 gcatttacgg cacagcggaa taacaaacaa atcatcagcc tttgttccca tccctcccag
     3361 tccataacca atgatgtgat gcggatcatc tgcctgattg ccacacgtca tgcatttctg
     3421 cgtttttacc caacgcgtgt atacaggcat ctcttcccgt tgtgatttct ggcgctggag
     3481 atactgagcc ggtgactccg gatcaacggc aatgctgacc accgtctttt cctgtggcgg
     3541 gttttgctgg tgggcgtgag gcagcggcgc aagatttttt gtgcgctgct tcagtatgct
     3601 ggtggcggtc tgctctcccg gtacgatgtc gctttcacgg tacattgagc ggattttttc
     3661 cgcacgcaac cccagcgaac gacgtaatac cgcttccggt agcgcgtccg ccacctgatt
     3721 gcggaccgcc caccaggata attcagccag cgataattcc cgttcctgcg agccattcat
     3781 tgcatggcgt atgacgtcaa tcatccatgc aaacaggttt tggtgagcaa gttgcccgag
     3841 tgattcggag gtctggtcgc gcagctggtt gtcgcagtgc cagcacaaca ccattgcgcc
     3901 ggtaccataa cggtgaatga cggtttcact gtggtgataa tcgccgtgtg gccactggca
     3961 ggatttaaca tggcgcagta accagtcaga caatgcgcca gcgccaccag cagcacgaat
     4021 cactcgttcg tcgctgaaaa atggcagtaa tgatttatcc tccgccagcg gctggcgaac
     4081 ggcaggaacg accccggacg gcagattacg catgcttttc ggttccggct ccaccagtac
     4141 ccgggtattg tggaataccg gcatggattc acggcccggc ttaacgatca ccagcccgag
     4201 ttccggtacc agaacaggtc gaagtaatac ccgcacgtta cctccagatg cgttgctgga
     4261 atgtgcggga cggacgcggt gggcgttcag agtaaggaag cctgacggag attatccagt
     4321 gacgataatc gaggctgagg gctttcttaa tctcgtatcc gcgtctgcgg tagttatgaa
     4381 ttagccattc ggcctgttct tcagtacatg gtgggtgttg gtaccagtcg gttttaaatg
     4441 cgtgtgaacg ccgcccatgc cggatggcaa ggtcggtatc agaattgtga aatttggttt
     4501 tgtgcgccat ctgttttctc tgctggcgca gcaggtgtca ggtgttcagg ctgacgtgcg
     4561 aattgtaaac cagaatgcca gaaaaaaaca aaacccgccg aaccgggtta agtgcgggtg
     4621 cgttgaggat gcctgacaca tcagaggtgg cgagggattt ctcccccgcc aggtctctta
     4681 ctcctcaggt tcgtaagctg tgaagacagc gacctccgtc tggccggttc ggattcgtac
     4741 ctcgcagagg tctttcctcg ttaccagtgc cgtcactatg acggttaaac agatgacgat
     4801 cagggcgatt aacatcgcct tttgctgctt catagcctgc ttccccttgc ctttcggcac
     4861 gtaagaggct aacctacatt tgtgagacat agattgggcc tcagattaat gttaagcgtc
     4921 ttgcaggacg cgaaatgtta actggggctt ttctctatct gcctttcagt gttcatgcct
     4981 gagacagata gcctcaagca cccgcagcca ttctacttaa ctcacgtcac ctcgccaata
     5041 tgaaatcaat cagaaaggtg atccataaaa tcactccttc tcttcttttc cgtagtggag
     5101 ttggccaatt ttgataagag ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag
     5161 agaatacaca cagccacaat attcctgctg atagaatttt tcgcgcttgc tgatttcaat
     5221 catacgggac gagccgccct gcttgcgcca gttataatcc cagtacacca tacccggata
     5281 atgcgcaaca gctcgccgcc cacactcgtt aacctgctgc atatttttcc agcgtgaaat
     5341 gcccagtgaa ctgctgatca cactgaaacc attttcagca gcgtacaacg ctgtccgctc
     5401 aaaacgcatg tcaaaacaca tggtacaacg gatccccctc tcaggctccc attccattcc
     5461 tttggcacgt tcaaaccagt tgtcggtgtc gtaatcagca tcgataaacg gcacgccgtg
     5521 ttgttcagca aagcgaatat tttcatcctt acgaattaaa tactctttct gaggatgaat
     5581 gttcgggttg tagaaaaaga tggtgtagtc gattcccgag gcctgaagcg cctccatcac
     5641 ttcaccggaa catggagcac agcaagagtg cagtagtagt ttgtttgccc cgtttgggag
     5701 ctccaattta ggccgtttga aatcagcaat agtcataaat atttttattg gggtcatgaa
     5761 aatagcacag agtgtagcat cagagcaggg ctatcgggaa tatatgtcta aatctggtaa
     5821 tatctggttt tgacgcaaag cggacaacca cgctggctct accctgcgcc atgaaaatgt
     5881 caattcacat ctgaactaat gctctttaat ctagtaacgt ctaaaatacc taacatttcc
     5941 ttgataaaat gccagtacac gctgcatagc ttcgctcttc cggcactcgc gacagattat
     6001 attcaggcgc ctgtcgtagc ggcgtatttc gccgtctggt aacgaccaga taaggtccgg
     6061 atcaaccact gcaggtttct tcacctttgc ccttgagagt tttttgcgag cattttgcca
     6121 gtccttacgc gcctgttcag acgggaataa cccgtaacca gagttgtata catcgccact
     6181 ggcaaccagc tctctggcga gaacactcat cagatatctt gtcgcacctg tcttggcttc
     6241 cagttgccgc aacgtctcgc gaccgctcag acgtacaagt tcaacaacct gccctttaat
     6301 tttttcccgc tcttcttgtg taaatacttt tgccataagc gcctccggca atcacttttc
     6361 cgatacaaca cggcgggaag aatcagtaat ctgtcgaaca atatcccggt gcttgttcag
     6421 ctcccgcagc gcggcgcaga ctcgctccca cttctgaaca tcacttttcg ccctgcgcag
     6481 cgccaggttt gccctgcgaa gggacggaaa aatcagctca tctgcttgcg tttcggtaaa
     6541 cgatggcaac ggctgcacaa tgtccgccac agtttctgtt ttaatttctt cctgtgttgc
     6601 ggcttcccgg actggtaacg cagcacctgc tggctgagga aaggccttac catcactttc
     6661 cgttaccagc gcggctttcg gctctgctgg taaattatcg cccggcatgc agtaacgaaa
     6721 tttaccgttc tgattaacgc gtgccagccg ccccgttgcg gttaccaccg ccagcgtgga
     6781 agcaaccttg cgagtactga caccgaactt acccgccagt tcctcacacg ttttagcccc
     6841 atcctgaccg ataaactcaa tcatcatgtc tgcggtaact ttttgttcga cctccccggt
     6901 cagcatatcc tgtgcttcag attttactgg ccgctcttcg gttacccggg attcaccttc
     6961 gccagccaga aaccaggtgt gaccagtttt atcaacgacg ccttttcttt tgagttccca
     7021 cagctcgttg acagcctctt cacgactgat tccaaggcga gctgccacca catgtgaaga
     7081 ggcttttttc agtgctttca gtgcgtcaga tacggtttcc attaaaattt cctccggaca
     7141 aaattacttc acaaccctca tattgctgac atttggacgc cagctatccc agttaaacgt
     7201 cacccatcga ccaccgttca tggtcatgcg gtccataatc ctctcaccaa gaagcgtact
     7261 cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg ctgtccggcg
     7321 atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa cgcctatttc
     7381 atccaggacc agcaaatcaa ccccgcaaag ctcctgtatt tttccccgga tttgccgttg
     7441 tcgtagctgt catgcaacac gctcatgacg tcagacacgg tgacgataat cacgctgcgc
     7501 cccttcacca tcagccggtt gcccatcgcc gctgcaaggt gatttttccc ggtgccggtt
     7561 ttaccgctga acacaaaatt cgtgcacccg gtcatcagtt cgtcagctat ggatttggcc
     7621 tggctcagcg cgtatttttg cccgtcgttc tgcacctgat aatttgcaaa cgagcatttg
     7681 ctgtgcagag gctggatgcc cgaacgattc aggatttttt ccacccgcaa ctggcgattc
     7741 tggcggttaa tctcctcgct gcgttttcgt ccttcagcaa gttgccattc ccgccactcc
     7801 tccaccgtcc ggtacggtgg aaccgacccc tgtggtgcaa gtctgcgaat acgttcaaga
     7861 accccaactg ccgcaatgtt tttcatgaca cgtcaccccc tgaatcccgg cggtatttca
     7921 gtgtccggtt cagaaatgtg attcacacaa cgctgcgcag gcgaacgccc caggcggata
     7981 accagttcat cccatttttc ccggagtttt gccggactca tgatgttttt tacccagaac
     8041 gaatcccgct ggagacgccc aaacatttca caaatttgtc tgtgagttct gccatccagc
     8101 atccgcattg tgcgaacgtc attggcccat gctgtccagt tgggttcttt cggtctagtg
     8161 atctcgccat catagctggc cgcctgctcg taaagactca cgattcgtcc ccagatccac
     8221 tgtgcgcaca ccaaatcttc ctgacttccc cactggcgtt ttttcgcact gaacacaacc
     8281 gcgtcagggt gtcgggttaa aaaatcctgt tcagccgtct gcgggtccgg ttgcgaagcg
     8341 tccggacaag aagatctttt atctgacgga tcaggtttta atactgacgg atcggggtca
     8401 atcatcgccc ccctaatcgg cagtttttta tcaacagttg atccatcaaa atttgacggg
     8461 tcaaccgttg aggggtcaat atttgacggg tcaactgtta acgggtcatt ttttgccggg
     8521 ctaatttttc ttttcggttt atatgactca cgcgccgccg ccgcagctgc ttcgagtttt
     8581 tccacattaa gccgatagat attgcttaca ttacgcccac cgaccttacg ctcttccttc
     8641 gtcagccagc cctctttcgc cagttctgca atagccgatt tcactgtgga ttcacttctt
     8701 gcaccgatct gacgccggat agtttcaatg gcaggccatg acacgccctc gtcattgctg
     8761 tagtctgcaa gacgggccat aaccgccacc ctggataaga tcatgccggt gaaggcgcac
     8821 ccttcccaga caagaccatg aagcttgctg ctcataaaac ccccgaacac cgtgctttta
     8881 gtgcatcacc acagcattcc ctgccgggcc gccgcgattc atctggtcat acaaaacaac
     8941 cgctgacgca acaaaatcat cgacatcctt caccagccga tccctccgtt cgacgatctc
     9001 acggtaatat tcagaactgt ggctgcgcat acgggccacc agcaaaggcg gcatcgcctt
     9061 ttcgatcgcc ggtaacagag cctgcatttt ttcaacagca tcaggggggt ctttatccag
     9121 ccaacggaaa attttctggg tattacgggc cagggcttcc ggatggctgt cgtcgtacag
     9181 ttcagggaac gtcatcccca gttcgaaata agtccgggct atttcagctg caggaacttt
     9241 ctcaccatca gggtatgccc aggcattcat cgccatgcgg atgtgctcat gtttgatttt
     9301 catgaatcat ttgcctcttg atgcttcggg tatgatcgtt ttcgtcattt ggttgcttca
     9361 tcgacatatt ctgcgaataa catgacgagc gtcgtaagta tgtccaatca acatcaggac
     9421 gaagttcttc acacaggaca ccaccttttg ttgctcgttc aatcgcagga catctctcag
     9481 caggtaactg acgtacacct ttgatccatt gatttacgct tggaggagat acacctaaaa
     9541 gcctagccat tgctgattgc ccaccgacaa cagcacaagc tcgtttgaat gaatagttat
     9601 cttttttcat cgaatgaact ccaaaaaaca cgcaacaata ttaggcttag cctaatgaaa
     9661 ttgtcaatag gctatgccta atacatcgag agtagggatt gcctaacgcg atgcgcatag
     9721 gagactatta agcaatgctt agtggtaaag acttaggccg agcgatagag caggccatta
     9781 acaaaaaaat tgcatcagga gccgtcaaat caaaggcgga aatcgcacgt catttcaaag
     9841 tccaaccacc atcaatccat gactggatta agaaaggttc gataagtaaa gacaaacttc
     9901 cagaactatg gcgtttcttt tctgatgtgg ttggtccaga gcattggggg cttaacgaat
     9961 accccatacc aaccccatcc acttcagata caaaaagtga acttttagac ataaacagcc
    10021 tttatcaagc cgcctctgat gaaaaaaaag caattgtggc tttcatctta tctggaaatg
    10081 ctacggagcc tagttgggtt gattatgacg ttcgcgccta cattgccgca atggaaatga
    10141 aggtagctaa ctatctgaaa aatcaagaat caaaacggaa aagccagaac atcaccaaga
    10201 caggaactta aacttatatg gtccgacggg aaattcctag ttcccgttag ttaactccta
    10261 ctacctctcc cacaaaccat cacctattag gttgcgccca aattattagg catagcctat
    10321 tgacaagtaa ttaggcattt cctatagttt tcccatacca acccatcccg tcccacacaa
    10381 tacagggcaa tacctcgagt taccaggcag tggtcagggg ttaagtagcc agcccgaggc
    10441 gtaagaacat gacggcaggg ttcaacttta ataactatgc agcaggtttt tgttccgcta
    10501 ccccggcgtt aaggggaaat gaggtcagca tggatactat cgagcttggc aacagcgaat
    10561 ctctggtatg tggcgtgttc cccaaccagg acggtacgtt caccgcgatg acgtatacca
    10621 gaagcaaaac gtttaaaact gaagctggcg cgcgtcgctg gttaaccaga aacactgact
    10681 gatgaggttg acgatggaat ttaaagattt accagtacca ttccaggaaa tggcatcgaa
    10741 tgtggttcgc tctcaactgg cgactcttga cctgagtacc gtagaaaaag aaaccatcga
    10801 caatatatcc ggtaacgtac gccgagcctt tatcgggctg tacgaagaga agctgctctc
    10861 tgataaccag gatttacatg aaaaatactt cctggatcta atggacatca ttgataaggg
    10921 gtttggcttg ttaatgaaaa agaaagggat tcgaatagaa ccccttgaaa attactttgc
    10981 aacaaaaagc attaattctt ttgattcaaa ataagagaat taattacaga cttaacatgc
    11041 tctttctcat gattgaagct ctcatgattg aaagtgccgg gttgaagcga gtcgatataa
    11101 tcaacaagac tctgtcgtac gacttcattt ttatccataa cagatgcaag aaatgaaatt
    11161 gctaaaagag ttatatcact acgcgccgca gcatgttgca atgctttatc aaaattatta
    11221 atctggcgta tcagggagtt aatgatttca tcattttcag tcgacatttc accctcctga
    11281 gggttggtga ttaaggagtt ctccacgggt gaggtggagt gcgtgcgccg gacacgggtg
    11341 agcatccggc actgacagtt tactgaaagg atatttccct gaaaagtcag accataacgc
    11401 gaaagcgccc ggcgaggtag ctggttcata gatagcctgt cgttaaattt tcgtcgaccg
    11461 tgcgtttccg gttgtggcaa tccgcgaaat ggcgcggcgg taagtatggc ggggttattc
    11521 cttccccgtt gaggacaccg agttgtcagg ttgaccatac gcttaagtga caaccccgct
    11581 gcaacgccct ctgttatcaa ttttctggtg acgtttggcg gtatcagttt tactccgtga
    11641 ctgctctgcc gcccttttta aagtgaattt tgtgatgtgg tgaatgcggc tgagcgcacg
    11701 cggaacagtt aaaaccaaaa acagtgttat gggtggattc tctgtatccg gcgttaattg
    11761 ttaactggtt aacgtcacct ggaggcacca ggcaccgcat cacaaaattc attgttgagg
    11821 acgcgataat ggaaacgtta ttaccaaaca ttaatacgtc tgaaggttgt tttgaaattg
    11881 gtgtcactat cagtaaccct gtatttactg aagatgccat taacaagaga aaacacgaac
    11941 gggagctatt aaataaaata tgcattcttt caatgctggc ccgtttacgt ccgatacaaa
    12001 aaggatgctg gcaatgaata cagcatttgc acttgttctg acagtttttc ttgtttccgg
    12061 agagccagtt gatattgcag tcagtgttca caggacaatg caggagtgtg tgactgcagc
    12121 aaccgaacag aaaattcccg gtaactgtta cccggtcgat aaagttattc accaggataa
    12181 taacgaaatc ccggcaggtc tttaaaacag ttccgtaata aatatccggt ttcattctta
    12241 tatgccagca atggcaggga tttgttcacc cttaaatctg taatgaggta aaacaaaatg
    12301 agtaaagtct ttatttgcgc cgccattccg gacgaacagg caataaagga agaaggtgcc
    12361 gtcgctgtag ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac
    12421 tggcaattcc tggaacatta tccggctgct caggactgcg cttataaatt tcttgtctgc
    12481 gaggataaac ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag
    12541 gaaaaccgct gggatgagga gtctgcttcc tttgtcccgg ttgagactga atcagatccg
    12601 atgaacgtca cttttgacaa gctggcccct gaagtacaga acgctgtcat ggttaagttc
    12661 gacacatgtg aaaacatcac cgttgatatg gttattagcg cacaggaatt gttgcaggaa
    12721 gacatggcaa cattcgacgg acatatcgtt gaagcgttga tgaaaatgcc agaagttaac
    12781 gccatgtatc cggagcttaa gctgcatgcc atcgggtggg ttaagcataa atgtaagcct
    12841 ggtgccaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
    12901 gaacgcaagg aaaccggaaa atacacgtct gttgttgatc tcgcccgcgc cagaaccaat
    12961 caacagcaca gtgaaaattc aacaggaaaa atcagcccgg tcattgctgc cattcatcgc
    13021 gaatacaagc agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt
    13081 gatgtggatg ccggaaacat tgacggcagc atccatcgct gggcaaaaaa tgaagttatc
    13141 gacaacgacc gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc
    13201 cttcgctacg accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac
    13261 aaagaccctg tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt
    13321 gaagatgaag gaagaaatca gagcgcaact gatactctct cgtcgccagt accagaaact
    13381 gatgcagtgg aaacggcaat tccgggcaac gaaaaaaccg aatgcaaagt ggaagtcgaa
    13441 ccatctgtag agcgtgaggg gccgttctac ttcctcttca ccgacaagga tggcgaaaaa
    13501 tacggtcgcg caaacaaact ttctggtctg gaaaaagcac tagccttggg agctacggaa
    13561 atcacaaaag aggaatactt cgcacgtaaa aacggcacgt actcaggttc acaacaaaat
    13621 actggtgcat ctgacacgac cgcacaacca gagccggtaa aagttaccgc tgacgaagta
    13681 aacaaaatta tgcaggcagc caatatcagc cagcctgacg ccgataagtt gcttgctgcc
    13741 tctcgcggag aatttgttgc agggattagc gacccgaatg atccgaaatg ggtaaagggg
    13801 attgaaaccc gcgattctgt aaaccagaac cagcaagaat cggaacagaa cgaccagaaa
    13861 gcggaacaaa acagcccaaa tacgcaacaa aacgagccag aaacgaaaca acctgagcca
    13921 gtagcgcaac aggaaccgga aaaagtctgc accgcctgcg gtcaaagcgg tggcggcaac
    13981 tgccctgatt gtggcgcggt gatgggtgac gcaacatacc aggaaacatt cgatgaagag
    14041 aatcaggttg aagttcagga aaatgatccg aaggaaatgg aaggcgctga acatccacac
    14101 aaggagaatg ctggtagcgc tcaggatcac gccagcgata gtgaaactgg cgagacggca
    14161 gatcccttaa ttgcgatgaa cggtcatcac gttatcacat ccaccagcag gacgtgtgac
    14221 catctaatga tcgaccttga aaccatggga aaaaatcctg atgccccgat tatctcaata
    14281 ggtgcaatat ttttcgatcc gcaaaccgga gatatgggac cggaatttag taagactatc
    14341 gatctggaaa ctgctggcgg agtcattgat cgtgacgtca ttaaaaggtg gctgaagcaa
    14401 tcacgtgaag cgca
//
LOCUS       DAJTYD010000093        14225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTYD010000093 DAJTYD010000000
VERSION     DAJTYD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.093"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="OO585_002515"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="OO585_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6630067.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="OO585_002516"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="OO585_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6630068.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="OO585_002517"
     CDS             complement(2788..5901)
                     /locus_tag="OO585_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6630069.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO585_002518"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO585_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6630070.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="OO585_002519"
     CDS             7143..7421
                     /locus_tag="OO585_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630071.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO585_002520"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO585_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6630072.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO585_002521"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO585_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6630073.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="OO585_002522"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="OO585_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6630074.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="OO585_002523"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="OO585_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6630075.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="OO585_002524"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="OO585_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="OO585_002525"
     CDS             complement(11102..11632)
                     /locus_tag="OO585_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6630076.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO585_002526"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO585_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6630077.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..13826)
                     /locus_tag="OO585_002527"
                     /pseudo
     CDS             complement(12709..13826)
                     /locus_tag="OO585_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3949 a         3019 c         3155 g         4102 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac attatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttgggggggt agggtgttct ttatgtaaaa aatagtgtca
    13081 cctaaatttg cgtcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag
    13141 gttagagcga acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg
    13201 gcaaagactt tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt
    13261 tgatacaggc tttgaatatc ttttatatta gttggtaagt cagctcgttg ttcagcaata
    13321 aaattagtat ctgaaaatat ggtttggaca ctgaatgtta tgggtattcc ctcttctacg
    13381 ctaattttag ttatgattgc cttgtcgggt ttaggattag cgacaacaat gattcgacat
    13441 tcagcacttt tgtttttttc atatctaatg ttattaatct gattaagtat tgattgtgtc
    13501 tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga
    13561 accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt
    13621 tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaat
    13681 gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc
    13741 gttttaacat tatcggtatt tgttgtgcag ttataaatat tgccgtgata tgtatttatt
    13801 atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaag
    13861 gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat
    13921 gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat
    13981 ttgtgtataa aaaaagcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
    14041 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
    14101 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
    14161 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
    14221 taaaa
//
LOCUS       DAJTYD010000094        14166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTYD010000094 DAJTYD010000000
VERSION     DAJTYD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.094"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(88..879)
                     /gene="fimH"
                     /locus_tag="OO585_002528"
                     /pseudo
     CDS             complement(88..879)
                     /gene="fimH"
                     /locus_tag="OO585_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(899..1402)
                     /gene="fimG"
                     /locus_tag="OO585_002529"
     CDS             complement(899..1402)
                     /gene="fimG"
                     /locus_tag="OO585_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6630078.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1415..1945)
                     /gene="fimF"
                     /locus_tag="OO585_002530"
     CDS             complement(1415..1945)
                     /gene="fimF"
                     /locus_tag="OO585_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6630079.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1955..4591)
                     /gene="fimD"
                     /locus_tag="OO585_002531"
                     /pseudo
     CDS             complement(1955..4591)
                     /gene="fimD"
                     /locus_tag="OO585_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4658..5383)
                     /gene="fimC"
                     /locus_tag="OO585_002532"
     CDS             complement(4658..5383)
                     /gene="fimC"
                     /locus_tag="OO585_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6630080.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5420..5959)
                     /locus_tag="OO585_002533"
     CDS             complement(5420..5959)
                     /locus_tag="OO585_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6630081.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(6024..6572)
                     /gene="fimA"
                     /locus_tag="OO585_002534"
     CDS             complement(6024..6572)
                     /gene="fimA"
                     /locus_tag="OO585_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6630082.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(7053..7649)
                     /gene="fimE"
                     /locus_tag="OO585_002535"
     CDS             complement(7053..7649)
                     /gene="fimE"
                     /locus_tag="OO585_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6630083.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(8127..8729)
                     /gene="fimB"
                     /locus_tag="OO585_002536"
     CDS             complement(8127..8729)
                     /gene="fimB"
                     /locus_tag="OO585_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCR6630084.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10185..10901
                     /gene="nanC"
                     /locus_tag="OO585_002537"
     CDS             10185..10901
                     /gene="nanC"
                     /locus_tag="OO585_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6630085.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10921..12027
                     /gene="nanM"
                     /locus_tag="OO585_002538"
     CDS             10921..12027
                     /gene="nanM"
                     /locus_tag="OO585_002538"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6630086.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            12092..13072
                     /locus_tag="OO585_002539"
     CDS             12092..13072
                     /locus_tag="OO585_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6630087.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            complement(13080..13202)
                     /locus_tag="OO585_002540"
     CDS             complement(13080..13202)
                     /locus_tag="OO585_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6630088.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13284..14156
                     /locus_tag="OO585_002541"
     CDS             13284..14156
                     /locus_tag="OO585_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6630089.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
BASE COUNT         3965 a         3285 c         3107 g         3809 t
ORIGIN      
        1 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgggttca cagataaaac
       61 actctccagg aaacccgggg cggttcatac tgctcctaac gataccgtgt tattcgctgg
      121 aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgcgctggtg aaaacgacgc
      181 ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt aatcccccag
      241 gttttggctt ttcgcacaat aaacggtaag aggaattggc actgaaccag ggtagtccgg
      301 cagagtaacg gtgacatcac gagcagaaac atcgcagccg ccagtaggca ccaccacatc
      361 attattggcg taaatattcc acacaaactg gaaatcatcg ctgttatagt tgttggtctg
      421 tcgcaaaata agcacggcaa ttaatgagcc agctttaatc gccaccccgc ccgcactgct
      481 cacaggcgtc aaataaagcg ccaccggcca cggcttatcc gttctcgaat tataaacaac
      541 ccgcggcgtt tcgctggtgg tcggaaatgg atagctactg ccactatatt ttacggtccc
      601 ggaaaaatta gataacacgc cgccataagc cgagcctcgt tgcagtgtga catagtctgt
      661 aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc gaaagatcta cgaccaggtt
      721 ttgccccaca ttcacgacag gcgcaaggtt tacataaaca ttagcgctgc caccgccaat
      781 agggatagca gtaccattgg cggttttaca ggcgaatgac caggcattta ccgaccagcc
      841 catcagcagt acagcaaaca gggtaataac tcgtttcatt acaattatct cttcgggttc
      901 agctgtaggt ataggtgatg ctaatcactg cctgaatggt tccctgagtg gctccgccat
      961 ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa tcatccacct
     1021 gaactgtttt ggtttcgcca gtattcaatg tgttgccact gtcatcctgt agctctaact
     1081 ggatgttttg cgcggtcccc tggtttttat aatatccggt actgtcggct gccccgctga
     1141 agctggcagt gaccctcgac gttcccaccg gacaattagt caactcaagc gcaacatcat
     1201 gccaggccga tgccgccccg gcagacatca gactgaaaga ataaagatcg ccgagatcaa
     1261 ccgtggcatt ggtggtggaa actgtgcacg gtttggcgac gaccttaccg ttcaccgtga
     1321 tggtgacatc ggctgcctgt attgtcgcac ttgcgagcgc caatattgcc gccaataaat
     1381 acccacgttt gcgccatttc atgagcatct ccagttactg atattcaaga gtgaaggtag
     1441 ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt cgccattagc cgggcgtaaa
     1501 aattcaacgt atttggttta cccggcgtca gggtcgtcca cgaaatcgcg gacgatggag
     1561 cattaagggg tatctgattt tgctgctcat tcagaagctg tattcccagt cccgaagccg
     1621 ctgacaccgt attttcaagt gcaagcaggt tggcattgtg gctatctgca acgccggtaa
     1681 acccaacctt tacggcagaa acggcgttac cacagggtga cagcaaaata cgaaatggaa
     1741 cgacaggagt cgttgcgcca atgttgttaa attgcttcgc cgcgttttcc atcagatcaa
     1801 cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca tagccgcgga
     1861 tagtaatcgt gctatccgca gccaaaacgc gacttaccgc cagccacaaa aaagcgcaca
     1921 gaagataaaa aggtttgttt ctcatcacgc ccccttaacg acattcagct gatagctggg
     1981 ttaataactg ctgctgactc tctggtggca gttgataatt ggcgatacag tgagcatttt
     2041 cctcttctcc ccatttcacc tgaacttttc ccgctaaagg cattccgctg aggtaaacct
     2101 gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc atcgccccaa
     2161 acggcagcgg cttattattg tgggttagcg tcatgagcag ttttatccca acgcgcgctt
     2221 taaactctgc tcgcacgatc gccccacgag tgggaacaac gttagcgacc gcgttatcta
     2281 aatcgacgtt atcagccagg gtattggtat ccagcgccac tctgttttcc cgatattcgg
     2341 tggcataagg cagcacggca taaccgcgcc agtcggtacg cacccccgtc tggttttcga
     2401 cttttgcatc ttttgcgcca ggcgctttaa caagtaccac cgtatcgttt aacggctgcc
     2461 ccagcgttac gccattggca tgagccagta ccccaccgct gactccgtaa tagagctgct
     2521 taatatcatc gctatggctg taaccgatat tggcattgcc gtaaccaccg cgataattca
     2581 gcgtggcgta gcctgtactt ccgctattac catcgcctcc cccggcatag ccggtttgca
     2641 cgctatagct gaggttgttg tcttccagca acgtaccgta tacaccagcc agattggtca
     2701 tccgaccgtt gagatcgtgt gacacgctgt agctggcact ggcatgtcgc cactgagatt
     2761 tactgtcaga acgcagccag tggctgaaag gaatattgac gttaagcgct aacatctgat
     2821 cccgcccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag ttgatatctt
     2881 cgaacgcagt atttaatcca gcctagaatt gctcatcgac attactcgtt ccccaataag
     2941 tttgatggct accactcaaa tacagtgttg atgtgcgccc gagttgctga gtaacggtga
     3001 gctgtaattt cccgcgtttg ttataagcga ggttgtaata gtcggtgaat ttcggcttaa
     3061 cctgaataac tccatcctgt gtttcgatgt tgtagccatt cattcgactg tatgttgtat
     3121 cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc cactaactga atattcgtgc
     3181 ctgactcatt gagcgatttg ttatagagaa aacgcaccga ttgtccgtca tgctgactgt
     3241 catcgggaag tgtggaatta gcctgcgtca tatccacaga cagagcgccc agtgccccca
     3301 tgtttttccc gataccgaaa ttaaaagcac gataacgatc cgccagttgc gttccaccat
     3361 atattgtcca gccagccgga aggccgtgga gtaatgtact ttggaaaaag cggggttttt
     3421 cctgctgcgt atttccacta cggtattctc ctgccgtaat ggaataacga gtatgccctt
     3481 cacgttgcaa aagcgggact gacgaatagg gtacggtaaa aatctgcgtg ctgccgtcag
     3541 cctctttgat cgttacctgc aagtcaccac tattacctgc ggcatagata tcgttgatgg
     3601 taaaaggccc cggtggcacc gtactattat aaatgtcata cccattttgt ttaatagtga
     3661 cctgtgcagt accacgagca ataccgtgga tcaccggggc aaatcctctt tggctatcgg
     3721 gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca tcgaaaatat
     3781 cgccctgggt ataaccatca cccagcgtca gccgggaacg taacggtatt atgtctcgct
     3841 caagccaggt attgatatgc tgccatttat ttttgctacc tgatgatctg tcgctactgt
     3901 tataactcca gctggtattg tcgcgtaaac accacgcacc aatatttaac ccactctgta
     3961 ggtttaaata tgcataatgg ctgttacccc caatctgatt ctgtacacta tttccgctga
     4021 aattataatt gagcaatccg gcattaatac cgggatccca taactcagga ggaatataac
     4081 cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttcagtcgc tgctgaccaa
     4141 catccagatg cgcagtagcg tcatggatca ttgaggttaa tggcacgcag gcatcatctg
     4201 ccagcagatt cataccggag acagaagccg cattcagccc catactggca agttgcgcgc
     4261 gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg acatcacgcg
     4321 ttgccatata accattattc aaatagatat cgacgcgata cgtccctggc ggtaattctt
     4381 gcccattttc aaaacgcgat aaatcggcca cagcctgggg atcatccgct aaaaagcgcg
     4441 gattaaaata gagttcggca gatgacaaag gtgcctgtgc ggcaaaagca caggcgacaa
     4501 cgagccggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat tgtgtgtttc
     4561 gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat tacccggcag
     4621 tcaattcttt ttattcaggc gagaaattcc ccctgcgtta ttccattacg cccgtcattt
     4681 tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt cctgcatcag
     4741 aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa tgcattttca agaacccggg
     4801 ttccggcatt caactctgtt accgtcagat aatagggtgt cgggttaatc agcgtcagag
     4861 aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc aacgctaatt
     4921 tagccgggcg atagtacagt ttaatgcggc tgataattgc gaactgtagc gtattctcag
     4981 tcaattttga tttatccatt gacggaatcg ctttaacatt catccagaat aaactttccc
     5041 ggtcctgtgg caattggtta tttgttgcat caagaatacg caaggtattc tctttttttc
     5101 ccttcatcgc aaacagagga ggcgtcacga taaaacgacc atcctttaca ccatcggcat
     5161 tttccatcca tgattgaatt aaataggtac tattttcatc attatttgtc acggcaagtt
     5221 gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc acctaaggcc actcccgctt
     5281 cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc aagcagaatg
     5341 ttatttcctg cgatttcctt acattggcgt ttttattact cactgtcctg ttcctgttat
     5401 cacattatct gctgaacaat tactgatagg ttaaagagaa ccaggcctgg gcattggcga
     5461 tgccgccagt aacccaacgc ccggtagcac gatatttggc gatgaaatgt agcgaagtag
     5521 agcctgagta aagccgtttc cagtttactg gaggacgatt aatcggtacg aggtttcctt
     5581 catcatcaaa caacgctacg ccaatattgg tggctatccc tggcccctct cccacggaaa
     5641 gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca cgttcactca
     5701 ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta tcttccccta
     5761 ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt ttatcaccgg
     5821 cttcaatccg gcaagtttcc gcaataatga cgccctgaaa ttgcatattt ccgccgggca
     5881 gcgtagtatt ccatttattt ccggccagag caaacattgg cagcaacgac gctaatagaa
     5941 ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc ctgcccgtaa
     6001 tgacgtccct gaacctgggt aggttattga tactgaacct tgaaggtcgc atccgcatta
     6061 gctgcgccag gggttgcgac accggttgca taataacgcg cctggaacgg aatggtgtta
     6121 gtaccgttat tcagggttgt ttgctcactg aatgtcgcac catccagcgt cagcgcagca
     6181 cccgttctgt ccaggatctg tacaccaacg tttgttgcgc tacccgcagc tgaactctgc
     6241 agagccagaa cgttggtatg acccgcatca atcgccgtac ctaaaaaggc aactgcggct
     6301 ttagatgcaa cattggtatc gcaatcattc agctgaatgt taaaaccgac agcagaactg
     6361 gttgctcctt cctgtgccag cgatgcggta cgaacctgtc ctaactgaac gatttgatca
     6421 acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa gtgaacggtc
     6481 ccaccattaa ccgtcgtggc agcggccaga gccgctgtag aactgaggga cagagccgac
     6541 agaacaacga ttgccagagt tttaattttc atgctgcttt cctttaaaaa aactatttct
     6601 aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg actgccaaca
     6661 ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca tagaggaaag
     6721 catcgcgcac aaactttttc agtttatttg ttggcttaat attctattgt tatctttatt
     6781 tatagatttt tatattgcat gaggtggttt ttggagagaa gaatgaggaa gatgcgtcga
     6841 gccacagaaa cgttagcttt acatatagcg gaggtgatgt gaaattaatt tacaagagaa
     6901 ataatttaca tatcaaacag ttagatgatt tttgtcgttt tttaaaattt ttatgcttga
     6961 gaaaaaatac gtaacttatt tatgatatgg acaatttggc cccaattgtc ttgtatttat
     7021 ttatttttta actttattat caataagtta attcaaacct cttctctttt taatttttcg
     7081 tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact ggcggtataa
     7141 cgtacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg agtatctgca
     7201 ccacgctccg ccagttcata accgcaagca tgccttaaca tatgaggatg agtctgcgtt
     7261 acggttccag cttcaatacc ggcatcacga ataatgcgat aggcctgctg gcgagaaagc
     7321 cgactcccgc ggcgagaaat aaatatggcg tcggtccggt cagcgccttt ccagttagca
     7381 cgttccaggg tccagcgttc cacagcttca cgctcatcaa aacgtaacgg gtgaacggta
     7441 gaaaatccgt tcttcagtcg gcgaatattt attcgacctt cattaaggtc aagatcctga
     7501 taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa cagaataaga
     7561 caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc ctgaacttct
     7621 ttaccggtaa gataacgacg tttactcaca atagtagtac tcctaactga gatatatttg
     7681 aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata aaaagataac
     7741 cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa tatcatacaa aaataaaata
     7801 tcactgatgt gacaacatct attttcattg atttgattca atataacaat agattgtagt
     7861 agattagctt ccagttgctt aaaaaataaa gaaaaaagtg cgaaaacagg agaatttaag
     7921 tgatgtgtaa atttatgaat gattttgttt atttattttg aagaacataa accattgtat
     7981 tttgtatgga tatggttaac ttaatgctta ttatgataat taagaattat acttacaata
     8041 acatcaatta ataaatggtt aatcattttt gatatatcgt aagaataatg tagtttttaa
     8101 caccatccct ggtatctcaa ctatctctat aaaacagcgt gacgctgtcg tcctctggct
     8161 ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg tataccagac agtatgacga
     8221 atattgcgat gcccaagata atcctggata agtcgcgtat ctattcccat attcgccaaa
     8281 gcaaaaccac acgaatggcg taacatgtgc ggatgaatct ccagtgacaa cccggcatta
     8341 ccaccggaag tcgagataat atggtaaaac tgttgccgag aaagcggatt ccccttacgc
     8401 gataaaaata cccactcgct ctcagcatgc gggtacgaag tacggatact caaccagttt
     8461 tttaaagcct gaacttcttt attcaatagc gggggcgttg ttgaaaagcc tttttttaat
     8521 cgatggatat atatacactt tgccttaaga tcaatatccg aaatcctcaa tcgacaaatt
     8581 tcactcgccc ggaaaccatg aataaaacaa agcaaagtca gacaataatt acgtgctgca
     8641 tgaggcccgg tatttgctgc tttaaggagt gattcgattt cactatgggt caggaagttc
     8701 ctttttttgt tatcagcctt attcttcatc gtttttccct tataattaca gacgcgcact
     8761 agctgtgctg ggttagcaat aatccaacat ttttatcctg attatgttta taaaagcgaa
     8821 cgtttgctta ataactaacg taagtggacc agttcttctg agtgaactta aatggggtag
     8881 caacagttaa ttataataac gttgccatac aaacgctatg ctcttgcatg ctatgtacct
     8941 ttatatattt atcaatcgga tacgaatcta ataatacccc tcatcattaa tgcaactaat
     9001 cttatctata tatcatgtga tatgttctgt aacaagtaaa atcagcataa aaaattcagc
     9061 aaaatttaat attcgttcaa ctagccgtct cacatatgac attattagtc aactccattt
     9121 gccacaaaat ggctatttca tggaacgcag taatttctta gtggctatta tcatgctaat
     9181 tcttaggtcc ctagcgatta ttcctggctg attcgtcagc ttacgccatg agattgttac
     9241 attccttttt atcctggaga tatgggggaa cgcaggagat gattttttca tctacattcg
     9301 ttggtaaata tagctttggt cactacgact taaaccttcc tcaatcacag ccattcgccg
     9361 ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc
     9421 tctccagatc gacttcagaa gtgaccagtt acaagccaca aaccaggaaa catatttcat
     9481 gatgagaatt atgctcagcc tgatggcggg taaacactga ttttcaccaa gctcactgag
     9541 ttagcttgca aagcttccct tgtttagact ccaaataaat tatttataaa acaaacattt
     9601 aaacaaagat ctttagtttt tgaagaccac cgcaagtgtt cgtctggctt cacatggcat
     9661 cttcctctta gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct ggttttgttg
     9721 tatggattgc gtgatttttg atctggtata acaggtataa aggtgcacca agatagtcaa
     9781 tgagacaggg catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga
     9841 tgaaaacgca cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta
     9901 tgactctggg taaaatggat tgagtaagtg atatagctta cgaaaattca aatcaattaa
     9961 acatcagaag agattttata ctcaggtatt taatctggat ctctgtttat ttaaataatg
    10021 tgaaaagaga tttttcacag gagaccttat acaaaaaaat ataaaataca gctaccggtt
    10081 gccaaagaca ctataagcct ggcaaaaaat attacacaac ataaatgcta attgtttatg
    10141 cgggctttgt attgctttct gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa
    10201 tactttctgg cgtattatta ctgtgctttt cgtccccatt aatttctcag gctgcgacac
    10261 tggacgtacg tggtggatat cgtagtggaa gccacgccta tgagactcga ctcaaagtca
    10321 gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag taatacctgg aataccattc
    10381 atgataataa aaaggaaaat gccgcactca atgatgttca ggttgaagtt aattacgcga
    10441 ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg
    10501 gcacacgcta cggaccctac gtaaaactgt cctgggacgc gacaaaagat cttaattttg
    10561 gcattcgcta tcgttacgac tggaaagctt accgacaaca agacttatcc agtgatatgt
    10621 ctcgtgataa cgttcatcgt tgggatggat atgtcactta ccatattaat agtgatttca
    10681 ccttcgcatg gcaaacgacg ctatacagca aacagaacga ttatcgctat gcaaaccata
    10741 agaaatgggc gacggaaaat gcatttgttc tacaatacca tatgacgccc gatattacgc
    10801 catacataga atatgactac cttgaccgtc agggtgttta caacggcaga gataatttat
    10861 cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga gacagtcaca
    10921 atgaataaaa caataacggc gcttgctatc atgatggctt catttgccgc aaacgcgtct
    10981 gtattaccgg aaactcctgt gccatttaaa agtggtaccg gagcaattga taacgacact
    11041 gtctacattg gtttaggtag cgcaggtacg gcatggtaca agctggatac acaggccaaa
    11101 gataaaaaat ggacagcgtt agctgcattc cctggtggac caagagatca agcaacctct
    11161 gcatttattg atggcaatct gtatgtgttt ggcggcattg gcaaaaacag agagggcttg
    11221 actcaggtat ttaatgacgt acacaaatac aaccccaaaa ccaatagctg ggttaaattg
    11281 atgtcgcacg caccgatggg catggcgggt catgtgactt ttgtacacaa cggcaaggct
    11341 tatgttactg gcggtgttaa ccagaatatc ttcaatggct attttgaaga tctcaacgag
    11401 gctggaaaag attcaaccgc tgtagataaa atcaatgcct actattttga caaaaaagca
    11461 gaagattatt tcttcaataa gtttctgttg tcttttgatc cctcaacaca gcaatggagt
    11521 tacgctggcg aatcgccctg gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat
    11581 aaaacctggc ttattaatgg cgaagccaaa cccggattgc gaacggatgc cgtatttgaa
    11641 cttgatttca ccggtaataa tttaaaatgg aataagcttg ctcccgtctc atcaccagat
    11701 ggcgtcgctg gcggttttgc ggggataagc aatgattctc ttatatttgc cggaggggcc
    11761 ggattcaaag gttcacgaga aaattaccag cacggaaaga actatgcgca tgaaggcctg
    11821 aaaaaatcat atagcactga tattcatctt tggcataacg ggaaatggga taaatcgggt
    11881 gaattatcgc aaggtcgggc ctacggagta tcattgccct ggaataatag tctattgatt
    11941 attggcggtg aaactgcagg cggcaaagcg gtgacggatt cagttttgat ctctgtgaag
    12001 gataataaag tcacagtaca aaactaacgc ttcagggccc cagtaaaggg gccctgctat
    12061 caatttatca tttcaattac gcgaaaatta tatggacgca ataatatcgc ccgattatta
    12121 ctatgttctt accattgctg gtcagtctaa tgctatggcg tatggcgaag gactgccatt
    12181 accggccagg gaagatgcgc ctcatcccag aattaaacaa ttagcgagat ttgcgcatac
    12241 gcatcccgga ggcccgtcat gtcactttaa cgacattatt ccactaactc actgcccaca
    12301 cgatgttcag gatatgcagg gttatcacca tcctctggca acgaatcatc aaacacagta
    12361 cggcaccgtt ggccaggcac tgcatattgc acggaaatta ctgtccttta ttcctgataa
    12421 cgcaggggtt ctcatcgttc cgtgttgccg tggcggatcg gcttttaccg cgggcagcga
    12481 agggacatat tcagaacggc acggagccag ccatgatgct tgtcgttggg gaacggatac
    12541 tccgctatac caggatttag tcagcagaac gcgagccgca ctggcaaaaa atccgcagaa
    12601 caaattcctc ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca gtgactacgc
    12661 gtcacaccct caacacttta atcatatggt tgaagccttt cgtagggatc taaaacaata
    12721 ccattctcag cttaataata ttactgacgc gccgtggttt tgcggcgata ccacctggta
    12781 ctggaaagaa aatttccctc atgcgtatga agctatttat ggcaattatc aaaataatgt
    12841 tttagctaat attattttcg tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc
    12901 gcctgatgaa gacccggacg atttaatcat gggatattac ggttcagcgt accgctcacc
    12961 ggagaactgg acgacggcac tgcgaagcag tcatttcagc gcggcagccc gtcgggggat
    13021 tatttctgac aggtttgtag aagcaatttt gcagttttgg cgcgaaaagt gagcgttcat
    13081 taatttgatt tattcgcatc catcaacgct tttaactcct ccggcgtaaa cggtggtttg
    13141 cgtcggtgca acatcgcatg gcaattaggg cacacaagca gcaaaaaaac aaatgagatc
    13201 aaggtaatat atcttcactt gcaggcttga acagcagaaa atttgcgagc atgatcgcaa
    13261 ttatttcact gctagggata tcaatggctt ccagtaaatc gctacagcag gcaattgcca
    13321 atataaaaat ctggcataag ggtgaacagc gcgcaccgca taaaccattg ttattgctat
    13381 acgtattagc gggatacctg aatggacatc cacgcctttt cgattatgga tcggaaatct
    13441 acgaacctct gcacagttta ctggaacgtt ttgggccaca gcgttcacag taccgacctg
    13501 atatgccgtt ctggcgatta caaggcgacg gattctggca attacacaat gccgagcttt
    13561 gctcaacagc agggagcagc cgacaaccgc cagtgaagga actgaacgaa taccacgttg
    13621 caggcggttt cgatgaacag cactacgccc tggtaaccgg caataaaaaa ctcatcaata
    13681 ctctagcgca acagatactc gaagcgcact tcaccgagag tattcaggaa gaaattgctg
    13741 acgagctggg ttttgatctc cagcaaatcc gtaaacagcg cgacccgtta tttcgcaaaa
    13801 atgtattacg cgcttataac taccagtgcg cgatatgtgg ctttaatatg cgccacgacg
    13861 acaccaccgt cgcgctggaa gcggcgcata taaaatggaa acagcacggc ggtccttgtg
    13921 aaattcctaa tggcctggcg ctttgcgcaa ttcaccataa agcctttgat aaaggatcga
    13981 ttggcctgga cgaaaatatg cgggtactgg tatcagatgc agtaaacggc ggagggagcg
    14041 ttgagagatt attctgggat tttgacggga aaattattgc gttgccgcag gtgcgtaaaa
    14101 attaccctta tgaggggttt gtggagtggc atcggaatga ggtttttagg gggtaaggtt
    14161 gataac
//
LOCUS       DAJTYD010000095        14050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTYD010000095 DAJTYD010000000
VERSION     DAJTYD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.095"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1272)
                     /locus_tag="OO585_002542"
     CDS             complement(<1..1272)
                     /locus_tag="OO585_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR6630090.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAAVIE"
     gene            complement(1275..1805)
                     /locus_tag="OO585_002543"
     CDS             complement(1275..1805)
                     /locus_tag="OO585_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCR6630091.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1798..2694)
                     /locus_tag="OO585_002544"
     CDS             complement(1798..2694)
                     /locus_tag="OO585_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6630092.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCASFTISEGGRNE"
     gene            complement(2698..3027)
                     /locus_tag="OO585_002545"
     CDS             complement(2698..3027)
                     /locus_tag="OO585_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCR6630093.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(3045..3611)
                     /locus_tag="OO585_002546"
     CDS             complement(3045..3611)
                     /locus_tag="OO585_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6630094.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(3623..4258)
                     /locus_tag="OO585_002547"
     CDS             complement(3623..4258)
                     /locus_tag="OO585_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6630095.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(4251..4718)
                     /locus_tag="OO585_002548"
     CDS             complement(4251..4718)
                     /locus_tag="OO585_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6630096.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4856..5263)
                     /locus_tag="OO585_002549"
     CDS             complement(4856..5263)
                     /locus_tag="OO585_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR6630097.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(5260..5652)
                     /locus_tag="OO585_002550"
     CDS             complement(5260..5652)
                     /locus_tag="OO585_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR6630098.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(5649..5972)
                     /locus_tag="OO585_002551"
     CDS             complement(5649..5972)
                     /locus_tag="OO585_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6630099.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5975..6175)
                     /locus_tag="OO585_002552"
     CDS             complement(5975..6175)
                     /locus_tag="OO585_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR6630100.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(6175..6669)
                     /locus_tag="OO585_002553"
     CDS             complement(6175..6669)
                     /locus_tag="OO585_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR6630101.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6837..7583)
                     /locus_tag="OO585_002554"
     CDS             complement(6837..7583)
                     /locus_tag="OO585_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6630102.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(7629..8501)
                     /locus_tag="OO585_002555"
     CDS             complement(7629..8501)
                     /locus_tag="OO585_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR6630103.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(8498..8680)
                     /locus_tag="OO585_002556"
     CDS             complement(8498..8680)
                     /locus_tag="OO585_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR6630104.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(8704..9540)
                     /locus_tag="OO585_002557"
                     /pseudo
     CDS             complement(8704..9540)
                     /locus_tag="OO585_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
     gene            9695..11446
                     /locus_tag="OO585_002558"
     CDS             9695..11446
                     /locus_tag="OO585_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR6630105.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11446..12482
                     /locus_tag="OO585_002559"
                     /pseudo
     CDS             11446..12482
                     /locus_tag="OO585_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(12961..13551)
                     /locus_tag="OO585_002560"
     CDS             complement(12961..13551)
                     /locus_tag="OO585_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6630106.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADKHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(13615..13926)
                     /locus_tag="OO585_002561"
     CDS             complement(13615..13926)
                     /locus_tag="OO585_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6630107.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(13931..>14050)
                     /locus_tag="OO585_002562"
     CDS             complement(13931..>14050)
                     /locus_tag="OO585_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6630108.1"
                     /translation="GAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         2847 a         3905 c         3882 g         3416 t
ORIGIN      
        1 ttcgataacg gcagcaacgg tcgatttcgc aagaatatcc cgcccgactt ttgtcagggt
       61 tgccaggctg gcgacatcat tccccgtaaa atacggaaac ctgtctgccg cagtagcaag
      121 cccggccagc gccgtcaggg tggcatcttt cggttgctta cccgcaagcg cgttagtcat
      181 ggtggtcgca aaattcgggg cgtttcccag cgccgccgct aactcgttca gcgtattcag
      241 tgcgtcaggt gacgaatcta caagggcggc aatcgcagcc ataacgaaag ccgtgcttgc
      301 gatttgggta ttattcgttc cctgtcgcgc agctggcgtt gttggcgttc cggtcagcgc
      361 aggactgttt aagggcgctt tcttgttcgt ttcatccatt accgccttaa ccgctttcga
      421 tgtcgcggcc agtgtttcag acgtgctgtt ggtggcacta ctgagctgga ctatcccttt
      481 ttgtgccgtc gtggcgtcct gagcggtata ttttgcgtta gcaaggtcat acgcggcctt
      541 tactgctttc ggcgttgcag cctgcgtttc agacgtgctg ttggtggcgc tactgagctg
      601 gacaaggcct tttcgcgtcg tgctggcatc ctgcgcagta tatttaccgt tagcaagatc
      661 atacgcggcc tttactgctt tcggcgttgc agcctgcgtt tcagacacgc tgttagtggc
      721 gctactgagt tgaacaaagc cttttgcggt cagcgaggcg tccgggtgac gtcgtgactg
      781 ttcatgctct ttcagtttgt catccacgta atccactgtg gccatcacca tggtgttgtc
      841 cacggtaagc gccacggtgg cagtgctgga tacggtcaga atggtgcgaa atgtttgtgc
      901 acgaccggac ccttcggcaa cggctggctt gtaactttcg gcagtatttc ccaccgcgat
      961 taaatcgccg tgctcatcaa atacaccaat ttcccggatc cagaatccgc ccgtttcagg
     1021 aggaataacc agctccgcaa taatgcggtt ctgatgtgtt gcgtccagga tgacgcgatt
     1081 aacagtatgt cgccacacct catgcaccag acgggtctgc ttactgtctg gtgtgggcaa
     1141 cgtgccgcca ccgtcgccca cggccatatg agtcaggcgg acaggcttac catctggcgc
     1201 ggctacctga gctaattttt ttgcacccgt atcggtgata atggttttaa attttcgtgt
     1261 tgtggtactc atgcttaatc gcctggataa atggtaataa cttcaccgtc ataagttgcc
     1321 gccgctgcga aaatatcccc cggaatttcc tgaatgatat tcagccctgt catgtggcgg
     1381 ctgaccgggc gggcatcagc aatcaaccgc tccatttcca gatacatttc ctccgtcacg
     1441 ccactgtcca gcgtgccgac ttcaacggta aatgttcccg gttctccgcc gaactcccac
     1501 cactcagaca cgcgaatgag gtatcccagc ggctcaatgg cccggcgcag tgcgctgatg
     1561 gtccctttgt gtcggtgtat cagccatgca tcacgaataa cctgtcgctt tgtctcttcc
     1621 ggccagttgc gatcccagcg gtcaacggaa aacgcccagg cgagataagg cagcagatgc
     1681 accgggcagg tgtccggcga ccacagcgtg ttgaggtcta ccggaatgtc tgtaatgcgt
     1741 gttccgacgg cttcggcaca acgcatgaaa ttgctggctg atggtggtaa caacgaatta
     1801 ctcattgcgt ccaccttcgc tgatggtgaa tgacgcacag cgcgccgcct gtatgtcgct
     1861 gatggccata ttctgtgtgg gttcgattat ctccacgcgt tgcacaccgt gcacatgcag
     1921 tgcggcagca atggcggaca acgccacgtc ctgaccgata agcccctgct cagccagcca
     1981 cttcctgaac gacgattcag ccgcggccag aataggttcg gattccgggc cggggtaaaa
     2041 gtacagtttt gcattcagcc gccatgtcac gattctggca ctctgtacgg tcaggcggtc
     2101 ggccaccggg cgggtatcct ctgcattcag aacggcgcga acggtattaa gcaacgcctc
     2161 cgttgctgtg ccgtcgccct cagtggacag gatggaaacc gtcacgttgg ccggagacgg
     2221 gctgatggcc cgcgcatcgc gtaccagacc gctggcgctg cgtgcaaaat actcgtatgc
     2281 acctgacggg ccagcaacac tcagaccgtc atatgcccgt tgcgcccgta gtctcagcga
     2341 ggtgtcactt tccatcactg catcggtggt atccgttgcc ggagtgataa ccaggcgctt
     2401 tgtgttcata ttgcccgcga ggttgtccag gtctgtcccg gcgctgtggc ttagcatgca
     2461 ggcgcgtgca ccctcattga cccgctggcg taacagcatt tcacgaaacg acatggtttg
     2521 agcgataacg ttaaggggtt ccgattccag ctccagcgcg gcggaaacgg cttcacgctg
     2581 ttcggcggga taggacgcaa tcatcatggc ctttgtgtca gccagaattg cctcaaagtc
     2641 aggctccgcg atgatggcgg gttccggtaa ctgtgaaagg tcaacggcag gcatgattta
     2701 ctccctcagc gtgatggtta attcaacatt ctgcatggtc tgcatgacag tgcccgacag
     2761 cgtcaccccg gcgcggcctc ctgccttcca gacaacgtcg atagcgtcca gggcaatgcg
     2821 gggttcccat cgtgtcagcg caatcacggc agcactcatg cattgcagac gcgtggtgtt
     2881 attcatgggt tcgtcaatca aatcaggcac aaggctgcca tattcccgtc gcataacccg
     2941 gcttgccagc ggggtggtca ggatgtccct gactgactgt ttcaggtgct ccatatcgtt
     3001 caggtttccc gttccgtccg ggttcattcc tgtgtagcgg gttgtcactg cgggcctcct
     3061 gtcgaatcgc tgccaccttt aacgccaccg tgtttatgcg tatgcactgt gatgccgttt
     3121 gaggtgaaat taccgccgct gtgcgtgata ttgccgctca tctttccccc ttttgtgacg
     3181 tcaagcgtcg ccgttctcag aaggtctgtg cattccacga cgggcgtatc cagtgtcacg
     3241 ctgacggatg cctgcagggt ggctgttttc atgccgctgg cgctcagtgc gcctgcgtcc
     3301 gcgtcgtagc ggaacaccgc gccatccggc gcgctgacca cgatttcttt caggcttttg
     3361 ccgggggccg gaatggcatc actccacagg ctgccaatta tcatggcggt ttccgggttg
     3421 ccgccaatgc aggcaattac cacctgttcg cctggtgatg gcggcagcca cacattgaag
     3481 gctcccgcgc gagaggtgtt ccagcgcagc caacctgttt ccagttcgcc gctgcgaacg
     3541 cgcacgcgcc aggacttctc atcaacttca gagatgatcc cggtgcggat gatattgctc
     3601 agtagccgca tgagttctgc gctcaccgta cagcctccgc aatccggccc agcaccgtgt
     3661 tataaatcag gcgctcatct gcctggctga tacccagcag ctcacgtacc gggtaatcgg
     3721 tgaaaatgcc cggcgcaacc tgatcgcgct caccgaactg atgaacgcgt gcaatacgtg
     3781 cggccacgcc gctgtaaccc accgtcacac cggaagcatc tgcacgggct ttcaggtagc
     3841 gggcggtgcg cagttttacg aacatgggga cgcgctttgt gctgtcctgg ttgatgcgcc
     3901 gggtgcgtat ttccagaaag cggtcgatgt catcccggta aaacgtgcgg atgctgtttt
     3961 tatcctcatc ccacccggtg atggttcgcc cgtatttccc cgtgtcgtga tgccagtttt
     4021 tcagcgtgcg tgcttcgtta ttccagataa agcgaatgcg ctcctgtatc cgggttacgt
     4081 ggcgtctgcg tggtgtccat gcggtcccgt ccggcgcttt ctgtggccgg atacgtgcct
     4141 gctgggcgcg gcgtaaatcc tgtgccagct ttctggcgat gttattgatg gcctgctgat
     4201 tcaggctgtc gcggatggcc tcaaaggttt catccacgcg agtgaatgcc ttatccatcg
     4261 ctttcacccc acgtcacatc ctggaataca tgcgaccagt cgccttcgga agatggcagg
     4321 cggggttttg gctccagcag gtgttctgcc tgcggtgtgc cctgactgct gcgcgtgatg
     4381 cgaacgcgtt cccgcagggg gagcgtaaac aggagatcgg cgctgtcatc gtcattgata
     4441 acggcggaaa atttgatgtc ctgattacgc tcagggttga gcaacaactg tggctgattt
     4501 tcggataacc acgccagcag cggcagcgtg aggtcgtcca gctccccggc gtaatccatg
     4561 acaaacatca ccatctgata gcggtaaaca aacgatgggg tttctccggt cgtttcaatg
     4621 ttgccgctct ccacgaaaat ggtgaatttt tccgggttag cctgacacca tcggcatgaa
     4681 cgggtcatgg cttcacgcag ggaatcagtt ttcagcatgg ttgttatcct cgttgttcag
     4741 tcgttgcagc ctgcgctgtt ccagtaattc aatggcccgt ttatccgcgt tacaggtttc
     4801 cagtgcatcc agaaggcggt cgccccatat accgagattt ccccatgtgg gagtgtcagg
     4861 gaagggggga ggcgttaccg gtatggtcag cgtctgcggt ataagccgga ctgacggcgc
     4921 tggcagtggc gcgttctgcg tgcctgcgca gcctgtcagt aaaacgagcg tcaggcaaag
     4981 cgtgggcgca ttcatctttt gcaatatcgt tgcgtagctg ttcacgtctt acctctccgt
     5041 cctgattgcg ttgctgattt tccacgcgga gttgcgccag cacctgctgc atatcctgta
     5101 ccccggcgct gatgatattc agggtgtcgg cggtactttt cagggtgctg gcctgcgctt
     5161 cgtttctggc gttctcccgg cccagcgacc acgacagacg catggatgtt ccccatccgg
     5221 cagtcagaag gaaagcgacg ccaagcgtgg gccagagctt catgccggat aggctccgtg
     5281 tggtaactga aaatgcggtc cgtctttcag ggtcttccag tcgccgcccc attccaccgg
     5341 aatattcagt tcccggctgg cctgtctgaa tgctgctgcg attttttcgt acagcggcca
     5401 ttcccatgac atctggctgc cgatataagc cacaacatcc acggcatgcc ccgtaaggtg
     5461 gcggctgttc atggtctggc tcttacccgt ggccaccagt tgcttctggc ggtaacggct
     5521 gcgcaaccct tcggtgatac caaaatccac ttccgagatt tccagtgccc gtcgggtcac
     5581 tttcaccaga tcaggattta caccctgcaa attcttttcg ctccggctgc tgaatttaaa
     5641 tgtgttgctc attcgtcctt ctccttcacc ctgcgattaa aggccgcaat aaccttgtcg
     5701 cgtgctttct ctgcacccat aaaaccgatt gatgcgccga taaacgtcac ggcatcttca
     5761 ggaaacccga agaagcgcaa cgacccggcc acggccatgg caagaacgcc gcacgccagc
     5821 gatcccgtta cggtctgaac cagtgttcgt ccgtcataaa ggctcatcag cgcggaaatg
     5881 ctgaccgccg cgcctactgc atacaccgtt ggcaggtggt caaagagcca cgcaataacc
     5941 tgctctgtga tccctgtttg aatggtgctc actgctactc cccccacaac tgaatcattt
     6001 ctcgtttctt cttctccggc tccggcatct ccacttcctg cccggcgtcc agaaatacct
     6061 gctgacagag tccggggttg gcatccagca ccttttcggt gacgccctgc gtcgtgccgt
     6121 agtaccggaa acagagcgaa tccacggtgt cgccttccag tgccttcact ttcatcagca
     6181 caactccgca aagattcgcg ggcggcacag aatgtcagag atggcccagc tcacatcacg
     6241 ccacaaatcc gatgtctgta tatccagagc gtctgcccgg cggtcgccct tgtccgtagt
     6301 gtctgcatca cgataacgct ccagaatcag ggcgcgcgtg gcggtataaa cagcattgcg
     6361 ccagtgccag agattgacgc tttctccgtt aattacgggt gccggaacat cggccagcgt
     6421 ctgatggcca gctgcctgct gttcctgctg ccatgcttcc agctcgcggg taacgtgtgc
     6481 cacagccccg gtggcagtat gcagcaggcg ggaggtggtc acgcgccccg gcagtcgtac
     6541 cgccagacgc agctcgcgca gcacaatatc cggccagaat gcacccgctg aaatgcgggt
     6601 atcgccatca tcggtatcgg tgatgtcgtc ctctgcgggt ccgggttcag ttctggcaac
     6661 tatagtcatg gggttcactc ctgaaaaaaa atcgggcggt gggtgcgcgg tgtaaacggt
     6721 cacggagtca aactggaaca ccgcgcacgc cgcccgctga cggggtcagt cgttaaccgc
     6781 gcttcgcctt ctgcgtcgcg gtggtttttc gtgttgcagg cttccgcgtt gtctttttac
     6841 ttttgctgct ttttgctgct ttcgtcctgc gcctgctgtg cgctggcgtc ttctggttcg
     6901 gctgcggaat cggctttttt cagggcgcgg gaaagggttg caatctcgcg tttcacacct
     6961 gcgttcgggt tcaggtgcat tgcttcgcgc agcagcttca gtgacagggc catgctgtcc
     7021 gcatcactca ggccacggcg ggcaaaggcg cacgctttgc ataatttggc gcgcacttcg
     7081 tccggcatgt cctggtcggt gacaatctcc cggagggtgt ccagtggttc gataaaggcg
     7141 gacaaatccg cgtcggcatc cgtcccggcc tgcgtcagta ccggattaca gatttcttcg
     7201 gtcagtaccg tggcagcagt acggccaaag ttatccggca tgatgaggtt gtgacggact
     7261 acatacgcgc cgatacgcag cgcaagcgga agatcgccac agtcaatcgc ccacaccatc
     7321 agcgtggcaa tcacttcatc ctgctgcccg ccgtcagcct ccagcgttcc ctcaatccag
     7381 ccggaaaaat ccggcaacaa ctcttttttg atggcggctt tcgcgcttct ggcctgtacg
     7441 cccttaagcc gggcctgtgc cagacgcaga cgatacagca cctcttcatg cgcggtacgc
     7501 gcggcgtggt ccacgccttc attcgcccgg cctgcgcgct gtgccatcac gttctgccag
     7561 tgttgttgtg caggagtaat catttttgct ctccgttaca ggcgggcatg atgcccgccg
     7621 tgagttgatt agctgtcggc gaacttcagg ccagtaacca tcgcgcactt gccatagtct
     7681 tcaacgacat aagcgtcatt gatggactgg taggtggcga tgcggttgta ttccggctcg
     7741 tctttcatca ggcgacgcat tgaacctttc tgccagtaaa tcgacaggtt gttgaacgag
     7801 gtgatcagca tcgttgcatc cgggaagaac ggcgcaagga acacgcccag cccgccaatg
     7861 gtgcgcgatg acaggatgag ctgcccggcg agtaattccg cattgggatt ctggccgctg
     7921 atgctgttca gcacgggcag acgcagcgag ttaaacaggt tgcgccccat aatcaccacg
     7981 aggtcgtcag cttccttgtg ccattcatct agcagggatg aacgcgcgtc ctgtaccagt
     8041 gcatcagcgt tcgcatactt acccgcgtgc gccacggtgt tgtccatgtt gcgggaggtc
     8101 agcgtcacgt cattcataac gcgttcgctg gcgtcggttc tgatgtgctc cagccagccc
     8161 acgttaacgt cctgaagcag cttgttggtg ctgaagttgg actcatccgc gtgagacgtg
     8221 ccgttgaaac cgatcatgat gcggtcaagc gccacctgtc gggcaatctg tgcgctgatg
     8281 cgggactgaa aatcagggtg tgccgaccag gcatcaagct gcggatacga aataaacgtg
     8341 tcgtagttca cctgttcaca ctgatatttg cggtttttca gatcaaccac gttattcggg
     8401 ttacggcgtt ttgtgccgtc ataactggta ttcgtgcgcg caatcggtcc tgtggtatcc
     8461 aggaggattt tttcgccttt ctggtcggtc acgccgatca cgttattttt ttcgtaaatt
     8521 cggtactttc ctttgaggcg ttttcaaaac gctgctgcac cgcgggttcc acggtaaatc
     8581 gcgacaccag tgcagatacc gggatattat taagcgacac ctgctgcgcc atatagcaac
     8641 ccagcttgtt gcgggtaata tctgacatca ccagattcat aaaaaatttg ctcctttgtc
     8701 taatcagaag tcggccagct ggtcggaggc tgcgcccgtt gcggtgaagc ggttctgcgg
     8761 atcgccgtcc tgcgtgcgca gtttttcctt cagtgctgcc agctctgtgg tcagtgacgt
     8821 aattttctgg cggtcctgct gatggcgggt ttccagcaca ttaaaacggt cgataatgtc
     8881 ggcctgtgac gttgcgacgc cttccaccgc ttcctgaata cgggaaaaac tggcgtcatc
     8941 cgctttgcgg ccacggccaa taatccccat tacgcggtta aaccactggg tgccttcttc
     9001 ctggcgttgt tctgccattt cgatgatttc agactcgatg gcttcggaga taagcggcgc
     9061 ttcaccctgg atactgttaa acgtcatcac cgcctaacgt tgctgtgccg tgaatttcag
     9121 gcgctcagtg cccaggcttg ccggggtgtc ggtcatcgcc agcccgacca gataggcgcg
     9181 cccgttaacg gagaactgcg ggtgcagttc gatactggaa tagattttct tgccgtccgc
     9241 aacaagctgc ttcatgcgct cggtcggttc gatttctgca tacagcgcag tacgtccggc
     9301 cagcggacct tccgtaatgt cttccgtact cagtgcggtg acatcgccca ttgcggaaaa
     9361 ttcgcttgac gggcatggcg agagatagtg ctcaacgttc acgcgggcag cgtaaacatc
     9421 cgggttgaag ttctcggcgg cttcacgcag atgcaccgga ctgatttcac ggccatcaac
     9481 agttgatccg gagacagcca cgcgaaactt tttgcgggat gtcttttttt cattagccat
     9541 agtttttgcc cctctgactg gttcttcagt catgatggca aagcgtaaca ggctgataca
     9601 aagggctttt gttgtaagaa aacggccaga acagagggtt aaggagaaca gtttcgcgcg
     9661 cgggtaatct tcctgtaatt actcaggggg agcaatgatt caggacgctt ttgtgcgcca
     9721 gcgtgcgcgg caactttact ggcagggtta tccgcccgca gaaatatcac gtctgatggg
     9781 aataaacccg aacacgattt atgcgtggaa aaaacgtgac cagtgggatg aaacgccacc
     9841 cgggcagcgt gtcacgcagt ccatcgatgc gcgcctcatc cagcttactg aaaaacagaa
     9901 taaaacaggc ggtgacttta aggaaataga cctgctgacc cggcagctta aaaagctgca
     9961 tgatggccag ccggatgtga tggccgcagg aaagaaaggc cgggcgaaaa aactcaaaaa
    10021 tcatttcacg ccggaacaga ttgccgcact gcgggaaaaa atcatcagca ggctggagtg
    10081 gcatcagcgg ggctggtttg actccctgac cctttgcagg gaagccggga tacgtaacag
    10141 gatgatcctg aaatcccgac agattggggc gacctggtat tttgcacagg aagcactgct
    10201 gatggcgctg cgtgacgatg tggcgcaacc ttaccagcgt aaccagattt ttttgtctgc
    10261 gtcgcgtcgt caggcgttcc agtttaaaag cattattcag aaggccgcgg ctgaagttga
    10321 tgtggagctg aaagggggcg ataaaatcat cctctccaac ggcgcagagc tgcattttct
    10381 cggcacttct gctgcgtcgg cacagtccta tacgggcaat ttttattttg atgaattttt
    10441 ctgggtcagt cgctttgctg aactgcgcaa ggtggctggc gctatggcaa ccctcagcgg
    10501 actgcggcgc acctacttct ccacgccatc caccgaaacg cacgaggcat acgcctactg
    10561 gaacggcgac cgctggaacg agaaaaaggc ctcgcataaa cgccagcgtt tttctgtgga
    10621 ctggaaaacg ctgcataacg ggcttatctg ccctgaccgg acgtggcggc aaattgtcac
    10681 gctggaagat gtggttaatc acggctggaa acacaccgat atcgacgaaa ttcgtgatga
    10741 aaacaccgaa gacgagttcc tcaatctcta tatgtgtgag tttgtccgcg aaggggaatc
    10801 ggcatttaac ctgaatatcc tgattggctg cggcgttgac ggatacgacg actggaaaga
    10861 ctggaaacct tttgctcccc gcccgatggg gaatcgtccg gtatggattg ggtatgacgc
    10921 aaacggcagc agtggcaacg gcgacagcgg cgctgtgtcc gtggtggttc ctccgtctgt
    10981 tcctggtggc cgttttcgaa cggtggagac gcgacgcgtt caggggctgg agtttgaaga
    11041 acaggccaga gtcattgaag agttcacgtg tcgctacaac gtggaacaca tcggcattga
    11101 tgtgacgggc gggaacgggg aggctgttta tcagatagtg aaacggtttt tccctgccgc
    11161 tattccgtac accttcacgc tgtcatcaaa acggtcgctg gtactgaaaa tgctgcaaat
    11221 aatgcgtgcc gggcggtggg aatacgatcg cgccgaacgc gagctggtcg cggcctttaa
    11281 cgccgtgcgt aaggtgaaaa caccgggcgg ctttatcact tacgaaacgg accgagcgag
    11341 ggggatcagc cacggcgacc ttgcgtgggc aaccatgctt gctgtcatta acgaaccgat
    11401 tggcggcgaa ggagaaaacg agcgtttcac ggttatggag ttctgatgag cagaaaaaat
    11461 aaaaaagtgc gcatgagttc acgcattgat ctcgctgatg cgctcaggaa agaatcatcg
    11521 ctcagtgcat tcacatttga tggtccttat cgcctgacct gctggacaat atgtactgtg
    11581 ctgataacgg gcggtggtat gaaaccccgg tggactggta cggtctggca agagctgccc
    11641 ggcaaacgtc ctggcatcag tctgcgcttt attttaagcg caatgtattg ctcggttgct
    11701 acatcccgca cccgctgctt tcccggcagg atttctcggc gctggcgctg gactggtttg
    11761 tgttcggtaa cgcattcctt gagcttcgaa gcaatatgct cggcgaaccg cttaaattac
    11821 ggcacgccct ggcgaaatac atgcgacgcg gaagcgatct tgaatcatgg aggtatgtgc
    11881 aggatggcaa ggatgcgttt cagtttcgcc caggcaaagt gtgtcacctg atgaatcctg
    11941 acattaacca ggaaatctac ggcatgccgg aatatctcgg cgcattactc tcggccagcc
    12001 tgtctcattc ggcggacatg ttcagaaaac tgtattacga caacggatcc cacgccgggt
    12061 gcatcatcta catcggtgcc gcgcaggtaa accgcgaaag catggactcc ctgaaagaaa
    12121 cgctacaggg tgcgcgtggt ggtggtgcgt ttaaaaacgt gctcattcat gcgcccaacg
    12181 ggggcaaaga gggggtgcaa attttgccgt tccagcagat caccgcaaag gatgagttca
    12241 tgaatgttaa ggcggcatcc cgtgatgatg tgctggctgc gcaccgcgtt ccgccgcaac
    12301 tgatgggggc gatgccgggt gaaaaaagtg cgtttggtga tgtggagaag gccgcgcggg
    12361 tttacgcaat taacgagctg atgcccgtca tggaggccat gaagcacatc aatgactggc
    12421 ttggcgaaga ggtgatccgc tttaaccctt acgcactgtt agacatccag cccacatcct
    12481 gacgcgcttc gcttgtctgc tgcttcgccg gggcataaaa aatttatgcc ccgactctcc
    12541 agctcctgta tcagtcagat aatttcacga cgccttccag tttatcgcca ccatcgacgg
    12601 tcagactctt acgcaatccc accgcgttga ctgcatgttc tcgccgcctc agtgcgattt
    12661 tgacggcctt acctttcacc ccatcaaatc agaatccctc acgtattttt cacgctcagc
    12721 gtgagaaata cggccattct gtcgtgtcgc tgcgacatcg ttaagggaac gctatttacc
    12781 ccctgaaacg cgggctgttc ccccgtcacc tgcgcgcaga aaaagcgcgt ttttttgtgc
    12841 acgcacggat ccttgacgga tccagccgcc acgcgggctg gaagggcaaa aattcgttca
    12901 aaaaaattgt gcaaatgtgc agcagggcga tgtgcgatta tcgccctggt ttgaggggga
    12961 tcaggtgtta tttttatctg tcgtcagtgg gctaaaccat ccacaattta catcgcatga
    13021 ctgatgcgtt ctgtcctcgt gtttctcctg ccacaatgct tttccgccag cctttatgat
    13081 ggctctttca acatcccgct cgtatcgcaa gggctgaaat acgtcattaa tgaaatattc
    13141 gcttatgtcc ggaagcgata ctgcaaaatt atttgtttgt tgcgccgtcc tgctatgcag
    13201 caaagcggca tgctttaatg cgtcctgtgc tttctggaat ttaagtgcat agtcagtggc
    13261 gattttttcc agttcagcga ttcggttgtc tttggtttcc agccaatcaa gtagcgccaa
    13321 tacttcagga tccccgacat ccaacactgt tacgcgtgat ttttcataat gtttgtcagc
    13381 aagagttcgg ccaattttga aatctccatc atcaccataa ccggagcagg cataaacaac
    13441 ctgtgctcca gatattcgct gaattgacat ttcctcgccg caaatagggc atttcggtac
    13501 tggcacaggt gaataacgtt cacgtaatgc ctgatagttg atttcgctca ttaagtcgtt
    13561 ctccttgttt atgttctgag gtggagagca gggcgaactc gccctgatgt tgtgttattc
    13621 aggaaataac gcccggatat ttccggccat ctgactggtt atctgtgcgg ttgatactgg
    13681 ctgtgatgcg gggcgttctg tcctggtttg tgtcactgat aacgcttcat caccagccca
    13741 tgcagccagt cgataagcct ctgccggatt cattttcaga agtgccagcc cggccagaaa
    13801 agccacgcgt tggccgcttt tgcgggcttc tggtgtaagg ctgtccagcc aggcgcatgc
    13861 ttctccttcg ttcttgacgg cggtgggctt cagatagaaa cttatccgtc tggttggagt
    13921 cgtcattggt ttactccttg tccattgcgt acagcccatt aaccagagca aactgtggca
    13981 ccccgtccgc gatgaaagtc gcattaactc cgcaggcttc gcggatagcg ggtgccacaa
    14041 tctccgcccc
//
LOCUS       DAJTYD010000096        13585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTYD010000096 DAJTYD010000000
VERSION     DAJTYD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.096"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(242..1615)
                     /gene="cobA"
                     /locus_tag="OO585_002563"
     CDS             complement(242..1615)
                     /gene="cobA"
                     /locus_tag="OO585_002563"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6630109.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1634..2440)
                     /gene="nirC"
                     /locus_tag="OO585_002564"
     CDS             complement(1634..2440)
                     /gene="nirC"
                     /locus_tag="OO585_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6630110.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2567..2893)
                     /gene="nirD"
                     /locus_tag="OO585_002565"
     CDS             complement(2567..2893)
                     /gene="nirD"
                     /locus_tag="OO585_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6630111.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2890..5433)
                     /gene="nirB"
                     /locus_tag="OO585_002566"
     CDS             complement(2890..5433)
                     /gene="nirB"
                     /locus_tag="OO585_002566"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6630112.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5695..6876)
                     /gene="tsgA"
                     /locus_tag="OO585_002567"
     CDS             complement(5695..6876)
                     /gene="tsgA"
                     /locus_tag="OO585_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6630113.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7147..7719
                     /gene="ppiA"
                     /locus_tag="OO585_002568"
                     /gene_synonym="rotA"
     CDS             7147..7719
                     /gene="ppiA"
                     /locus_tag="OO585_002568"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6630114.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7824..7991
                     /locus_tag="OO585_002569"
     CDS             7824..7991
                     /locus_tag="OO585_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6630115.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7981..8583
                     /locus_tag="OO585_002570"
     CDS             7981..8583
                     /locus_tag="OO585_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6630116.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8615..9178
                     /gene="pabA"
                     /locus_tag="OO585_002571"
     CDS             8615..9178
                     /gene="pabA"
                     /locus_tag="OO585_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6630117.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9264..10484
                     /gene="argD/dapC"
                     /locus_tag="OO585_002572"
     CDS             9264..10484
                     /gene="argD/dapC"
                     /locus_tag="OO585_002572"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6630118.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10551..12641)
                     /locus_tag="OO585_002573"
     CDS             complement(10551..12641)
                     /locus_tag="OO585_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6630119.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12692..13324)
                     /gene="crp"
                     /locus_tag="OO585_002574"
     CDS             complement(12692..13324)
                     /gene="crp"
                     /locus_tag="OO585_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6630120.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3288 a         3494 c         3557 g         3246 t
ORIGIN      
        1 ttccctatgt attatatatt aattaatatt cattgattat atgaatataa gcagactgct
       61 cgttaagaac gatacaggaa tatttaattg gcgtcagtga gcgaatgact taattttgta
      121 agtcgctgta acggtgaata taacccatta gcagataata tttattaagg ggttatgttt
      181 tatttgctga atatataaaa agagtcatta agaaggccag aattctctgg cccgggttaa
      241 attaatggtt ggagaaccag ttcagtttat cgcgcaggcc aacaacccga ccaataataa
      301 ttagcgatgg actgttcatt tgctgagcaa gttcaccgag ttgcgtgagc gtaccgtcaa
      361 tcacgcgctg cgtgactgcc gttccgtttt cgacaattgc caccggcatt tcgccaggca
      421 taccgtgttc aatcagcttt tgctgaatag tcgcggcctg attcaacccc atatagaaca
      481 ccagcgtctg tttttctgcc gccaggtttt cccagtccag ctcgccaccg gtttttaagt
      541 gtccggtaat taagcgtacg ctctgggcat aatcgcgatg cgtgagtgga atacccgaat
      601 aggcagagca accagaagct gcggtaatac ccggaaccac cgagaacgga atacccgcgt
      661 tgcacagtgt ttccagctct tcgccaccac ggccaaaaat aaacggatcg ccacctttca
      721 gccgcaccac gcgtttgcct ttttgcgctt cccgcagcag gatctggtta atctcttcct
      781 gcggtacaca gtggtatccc gcgcgtttgc cgacgaaaac gcgatcagca tcgcggcgta
      841 ccagattcat aatatcgtca gaaaccagac ggtcgtagac caccacatct gcctgctgaa
      901 tttgttgcag tcctttcagc gtcagcagcc cggcatcgcc cggacctgca ccaaccagca
      961 ccacttcacc gcgatggtcg agcggttcgt tgattaactg ttcggtcgtt tcagtaatgg
     1021 ctttctgatc gttgtttgcc agcgactgcg ccaggcggtc gttaacgaac aatttctccc
     1081 agaaacggcg acgctcactc atcgtggtga actgttgttt cactcgcccg cgtaattgcc
     1141 cggcatattt cgccacctgg cccagatgca acggcagcag tgattcgagt ttttcgcgca
     1201 acaggcgcgc cagaaccgga gaggtgccgc cagaggagac cgctaccatt agcggtgagc
     1261 ggtcaataat cgacggcata ataaagctgg cggctttcgg cgcatcgact acgttacaga
     1321 agatgcgacg agcttcagcg gcttcgctga cgcgctggtt aagcgtgtca tcatccgtcg
     1381 ctgcaatcgc cagccagcag gtgtcgagaa ggctttcatc aaatggccct tcgacgaggg
     1441 ttaacatgcc tgcatctgcc catgcggtga actgtggaat aaacgctaat gcattcaccg
     1501 ttaagcgagc gcctgcgtct aacagcaacc ttgctttgcg ttccgcgaca tcaccaccgc
     1561 cgacaatcag acagtcgcga tcgcgtaatt ggcaaaatat aggcaaatga tccacgtaaa
     1621 aaccccttag taattaaccg gcagccgttt cagtttgatt aaatttgtcc gcaaccggac
     1681 gattcgattt cggcgtagca taccaataac ccaatcccat gaatacggca cctgataaag
     1741 tattacccag cgtcacccac agcaggttat gaccaatacc cgccagcgtg taggcttcgc
     1801 tgtggttgcc gaaccaggag agcgcgaaca gcgtcatgtt agcgatagag tgctcgtagc
     1861 cggacgcgat aaatgccagc agacaccacc agatagcgat aaatttcgcc gccccttcag
     1921 tgcgcagcgc catccagatt gccaggcaaa ccagccagtt acacaatgca cctttgaaga
     1981 agagtaccat tgccggtgca gtggttttag ccagcgcgac ggagtgaacg atgctggtat
     2041 ctaccggcag caggctaccg ccgccccagc tatagagcat ggcaacgaag acggaaccga
     2101 ccaggttacc cagccaggtt tgcggcagga ttgcccacat ttgcccgtgg ctgatgctgc
     2161 ccgcttttac cccaaaggtg aggaacatgg tgtgtccggt gaacagttca gaaccggcga
     2221 taatcaccag cgttaaggcg ataccaaagg tcgcgcccat caccagcgga cgtacggacg
     2281 ggtcgagcaa attaccgagc gtgaaaatca agatgatccc aagacccaca tacgcgcccg
     2341 ccatggcgga gctgacccaa aagccgagcg ggttatttgc cgacaggcgt gcaatgcgcg
     2401 cagcgttagc cgcacactta ttaatagtgt ctgtaaacat ttgattatcc tttaaaaaat
     2461 aaaaacaaaa aaataaaaca aaaagtttaa aaaatgtcct gcaatatacc gaaggtaatt
     2521 acaggaccat gcaaaaaggg gaggcattgc gcctcccgtt aaaacattaa ccgcgcagct
     2581 gcaccacgcc gtctttcact cgtgcttcgt aatgtttgac ggaaaactgt tcgtcttcca
     2641 tgcacaagcc atcacttaag cgaaaacgct gttttttcag cgggctggcg acccacagct
     2701 cgccctggtg ttccgcaatc agcccgcgtg acagcacgct ggactcgaag aacggatcga
     2761 tgttgctgat cgcaaacacc tggtcgctgt gatacgggcg gaaaatcgcg acctgctcgt
     2821 cgcctaacag cgcgcagacg ccggtttcag gcaggatgtc atcgattttg cagatgtctt
     2881 tccactggct catgcgttgt cctccaccag agttaccggg atacgttcat acggcgttgc
     2941 cggacggtgc tgttcgcgct ctggcaccat ctgcacgttc gggtcacgct tgtcgctgtt
     3001 gatgaagtgt ttgaagcgag tctgcgcgga cggtgtattg accgtttcag tccactcaca
     3061 cactaccgct tcacgcaggc gcgccatctc ttcttccaga tgagcgttca gccccagttt
     3121 gtcgtcaatg atcaccgctt tcaggtaatc gatgccgcct tcgaggtttt ctaaccacgg
     3181 cgcggtacgc gtcagtttgt cggcggtacg gatgtagaac atcatgaagc ggtcgagata
     3241 tttgatcagc gtttcgcgat cgatatcagc tgccagcaga tccgcatgac gcggtttcat
     3301 gccgccgttg ccgcaaacat acaggttcca gcctttttca gtggcgataa tacccacgtc
     3361 tttaccctga gcttctgaac attcacgggt acagccggag acaccgaact tcattttgtg
     3421 cggcgtacgg atgcctttgt agcggttttc cagttccacg ccgaggccga cgctgtcgcc
     3481 aacgccgtag cggcaccagg tgctacccac gcaggttttc gccatacgca gtgctttcgc
     3541 ataggcatga ccggtttcga agccggcttc aatcagctga cgccagatct ctggcagatc
     3601 gtctttctgt gcgccaaaca tcgccaggcg ctgggaaccg gtgattttgg tgtagagatt
     3661 aaattcacgc gcgatacgac ccactgccat cagcccttcc ggggtgattt caccgcccgg
     3721 agaacgcggg atcaccgagt aggtcccgtc tttctggatg ttagcgagga agttgtcgtt
     3781 agaatcttgc agcggagtat gttctggctt cagaatgtat tcgttccagc aggaggccag
     3841 cagcgaaccg acggtcggtt tacaaacttc acaaccgtag cctttgccgt gtttcgccag
     3901 cagttcttcg aaggttttaa tgccttcaac gcggatcaaa tggaacagtt cctgacgcga
     3961 ataagcaaag tgttcgcaca ggttgttgtt aacttcgatg ccctgtttcg ccagttccgc
     4021 gttcagtacc tgagtgacca gcgggataca gccaccgcag ccagtacccg ctttggtttc
     4081 agctttcagc gccgcaactg tgtggcagcc tttgttgatg gcagcaatca gatcaccttt
     4141 agtgacgtcg aagcaggagc agatttgcgc gctgtccggc agtttatcaa caccgataga
     4201 cggcttaccg ctacccgagt gcgccggcag aatcagagaa tccgggtttt ccggcagctc
     4261 gatagcattc agcaccagtt gcagcaagtt accgtagtcg ctggtatcgc ccaccagtac
     4321 cgcaccgagc agggttttgt tgtcttcgct gacgatcagg cgtttgtaga tctctttgct
     4381 ttcgtcgagg taaacgtagc tacgtgcgcc aggcgtgcga ccatgagcat caccaatacc
     4441 gcctacgtcc acgcccagca gtttcagctt ggcgctaagg tcagcacctt caaaggcgtt
     4501 ttcgctaccg agaatatggt caacggcgac ctgcgccatt ttgtagccag gtgctaccag
     4561 accaaatata cggttgttcc agcttgcgca ttcaccgata gcgtagatat ccggatcgga
     4621 agtttggcag gaatcattaa tgacgatacc cccacgcgga gcaacgtcca gaccacactg
     4681 ggttgccagc ttatcgcgcg gacggatacc ggtagagaag acgataaagt cgacttccag
     4741 ttcgctgccg tcggcaaaac gcatggtttt acgtgcttcg acaccttcct gcacaatctc
     4801 aagggtgttt ttgctggtgt gaacgcgcac gcccatactt tcgattttgc gacgcagctg
     4861 ctcgccaccc atctgatcaa gctgttccgc catcagcata ggggcaaatt cgataacgtg
     4921 ggtttcaata cctaagtttt tcagcgcgcc agcggcttcc agacctaaca ggccgccacc
     4981 aacaacggca ccgcgtttgc tgcgacgggc gcaggattca atggcgttga ggtcttcaat
     5041 ggtgcgatag acaaagcagt cctgagtatc agaacctttg attggtggga tccacgggta
     5101 ggaaccggtt gccatgatca gcttgtcata aaaaacggta cgtccggcgc tggagtgaat
     5161 caccttctcc tgacggttga tggtgatagc gcgttcgccg accagaactt tgatgccgtg
     5221 tttctcgtag aagccttcgc gcaccagcga cagctcttcg gcggtgtggt gagagaagta
     5281 agacgagagg tgtacgcggt cataagcgat gcgcggttct tcacagaaaa cggtaatatc
     5341 aaagttggcc gcatcagatt tatcaagaag atcttcgata aagcgatggc cgaccatacc
     5401 gttaccgata attgcgagtc tgactttgct catttttgcc tcgatttctt ttctattacc
     5461 gcctacctta acgattcagc aaccccgctt attgatgtaa atcaaattca cctttatata
     5521 ccccttaagg ggtatgttgc tgatttgtat aaattttctt aagtcacgga attgtctgac
     5581 atttcatgtt tgtgcaaaaa atagacaaaa tgaacgggtt tatagaaata gatgcacgta
     5641 gcggggttag agaggaaaag gccggacgac gatagtgccg tccggcatta gcaattaatg
     5701 agaggtcagg gtgttatgct gacggtgacg gctgacgaaa cctaacagga agcacatcac
     5761 aaagacgaca gcgtacagac cgtttgctgt aagcagtgcc gcctgcggac cgctatgttc
     5821 aacaatcggg ccggtaacca caaaggtcaa catagtaccg atggttccgc aggtcaggac
     5881 aaagttaacc agttttggcg acggtacttt ggtctgctgt gaacccaggg tgatgatggt
     5941 ggtatagatc gcgctggaga agaagcccag agcgagaatt gaccacgcca tatgtgctgg
     6001 tgttccggtg ttaaagacgt acatcagaat cgcagccaga ccagccagta cggtcagaat
     6061 gcgttgcaaa tcaaagaagc gaagaataaa gctgaacgcc cacatgccga ccatgtatga
     6121 catccagaag ttactcacca gcgtgcccgc gtcgttcagg ctcatgccca ggcctttggc
     6181 atactcaggc acccaggaga taaaacctaa ctgaccgagg atgtagcaca gcgccgcaac
     6241 agagagaaac agtacgccga tcccccactt ttctttctct accggagcat ccgtttttgg
     6301 tgcatgtttg cccagtgctg ggaactcaca gccgaaggtc agaataaaaa tagcgacata
     6361 caccagcccg atgcaggcat aaacccagta ccactcaatg ctgcgcgcca gtagaaacgc
     6421 ggcgatcatt gggaaaatca tcccagccat actgaagaag gagtcagtaa ataacaggcg
     6481 ggaaccgcgc tgacgccctt catacatttg tgttatcagg aatgtaccaa tcgacatagt
     6541 aatgccgctg accaccccga gaatgaacat cgccgtcgag aacagcgcca ggctgtggct
     6601 gaacatcaaa ccggcaaccg ccagcaccat caggagaaag ccaaaacgta actgcgtttt
     6661 caacgggacg atttccatca gccaggcgtt gaggaagata gagattaaaa tgccggcgtt
     6721 gaggaaggtg aaggtgttac tcatactgga aacaggcaga ttgaaataat cggcgatatt
     6781 tcccatcacc atcccggtga caataaccaa cgcaccagtc agtgcgtagg agagaaagct
     6841 aatccatgtg agcttgatgc gattgctgtt agtcatgatt ggcctgcgtt caaaaataaa
     6901 atggcatagc gggatatgcc gcgagcgggt gattttaggt gattttgtga tctgtttaaa
     6961 tgttttattg caatcggttg ctaaattgca ttttaagaag tgatcttgat cacggaataa
     7021 aaagtgatcg tcaggttaca tatatttcag atacgtaaaa ttaggtaaag ggatggcctt
     7081 gttcttgaag gctatttaga atctcttcac ttgctttttt tctgctctgt ttgttaagga
     7141 aatctcatgt tcaaatcgac cctggcggcg atggctgctg ttttcgctct ttctgctctt
     7201 tctcccgcag caatggcagc gaaaggggcc ccgcacgtat tgttgacaac ctcagctggt
     7261 aacatcgaac tggagctgga taaacaaaaa gcgccagtgt ctgtgcaaaa ctttgtcgat
     7321 tatgtgaaca gcggttttta taacaacact acctttcacc gcgtcattcc tggctttatg
     7381 attcagggcg gcggtttcac cgagcagatg cagcagaaaa aaccaaaccc gccaatcaaa
     7441 aatgaagccg ataacggcct gcgcaacacg cgtggcacca tcgcgatggc gcgtaccgct
     7501 gacaaagata gcgccaccag ccagttcttt atcaacgttg ccgataacgc cttccttgac
     7561 catggtcagc gtgatttcgg ttacgcggta tttggtaaag tggtgaaagg catggacgtg
     7621 gccgataaga tttcccaggt gccgactcat gacgttggtc cgtaccagaa tgtgccgtca
     7681 aaaccggtag ttatcctttc cgctaaagtc ctgccgtaat gatttctcgc gcgggcaatc
     7741 ttgcccgcgc ttctgctctc ccggcgtaac ccggatttgc cgcttatact tgtggcaaat
     7801 ggacacgttc agggaggcat caagtgaaga aactcaccga taagcaaaag tcccgtctct
     7861 gggagcttca gcgtaatcgt aatttccagg ccagtcgccg tcttgaaggc gtcgagatgc
     7921 ctttagtcac tcttactgcc gcagaggctt tagcgcgcct tgtagagctg aggaggcact
     7981 atgagcgata aattcggcga agggcgcgat ccgtatcttt atccaggcct tgatatcatg
     8041 cgtaaccggc tgaacatccg ccagcaacaa cggctggaac aggccgctta cgaaatgacg
     8101 gcgctgcgtg ctgcgaccat tgagcttggt ccgctggtgc gcggtttacc gcatttgcga
     8161 actatccatc gccagttgta tcaggatatt ttcgactggg cagggcaact gcgtgaagtt
     8221 gatatttatc agggcgatac gccgttctgc cactttgctt atatagaaaa agagggcaat
     8281 gccctgatgc aggatctgga ggaagaaggt tatctggttg gcctggagaa aacgaagttc
     8341 gtcgagcggc tggcgcatta ctattgtgaa atcaacgtgc tgcatccctt ccgggtggga
     8401 agtggtctgg cacagcggat cttcttcgag caactggcga ttcatgccgg atatcaactg
     8461 agctggcagg gtatcgaaaa agaggcctgg aatcaggcaa atcagagtgg ggcaatgggg
     8521 gatctcaccg cactgcagat gatatttagc aaagtggtaa gcgaagccgg ggaatctgag
     8581 taaaatagcg cggttctttc gtaccggagc cgccatgatc ctgcttattg ataactacga
     8641 ttcttttacc tggaacctct accagtactt ttgtgaactg ggggcggatg tgctggttaa
     8701 gcgcaacgat gcgttgacgt tggcggatat cgacgccctt aaaccacaaa aaattgtcat
     8761 ctcacccggc ccctgtacgc cagatgaagc cgggatctcc cttgacgtta ttcgccacta
     8821 tgccgggcgc ttgccgattc ttggcgtctg cctcggtcat caggcaatgg cgcaggcatt
     8881 tggcggtaaa gttgtgcgcg ccgcaaaggt catgcacggc aaaacctcgc cgattacaca
     8941 taacggtgag ggcgtatttc gggggctggc aaatccactt accgtgacac gctaccattc
     9001 gctggtggtg gaacctggct cattaccagc gtgctttgac gtgacggcct ggagcgaaac
     9061 ccgcgagatt atggggattc gccatcgcca gtgggatctg gaaggtgtgc agttccatcc
     9121 agaaagtatt cttagcgaac aaggacatca actgctggct aatttcctgc atctctgatt
     9181 tccgattgcc attcagtgat tttttatgca tattttgtga ttataatttc atatttattt
     9241 atgcgtaaca gggtgatcat gagatggcaa ttgaacaaac agcaattaca cgcgcgactt
     9301 tcgatgaagt gatcctgccg atttatgctc cggcagagtt tattccggta aaaggtcagg
     9361 gcagccgaat ctgggatcag caaggcaagg agtatgtcga tttcgcgggt ggcattgcag
     9421 ttacggcgtt gggccattgc catcctgcgc tggtgaacgc gttaaaaacc cagggcgaaa
     9481 ctctgtggca tatcagtaac gttttcacca atgaaccggc gctgcgtctt gggcgcaaac
     9541 tgattgaggc gacgtttgcc gaacgcgtgg tgtttatgaa ctccggcacg gaagctaacg
     9601 aaaccgcctt taaactggca cgccattacg cctgtgtgcg tcatagcccg ttcaaaacca
     9661 aaattattgc cttccataac gcttttcatg gtcgctcgct gtttaccgtt tcggtgggtg
     9721 ggcagccaaa atattccgac ggctttgggc cgaaaccggc agacatcatc cacgttccct
     9781 ttaacgatct ccatgcagtg aaagcggtga tggatgatca cacctgtgcg gtggtggttg
     9841 agccgatcca gggcgagggc ggtgtgacgg catcgacgcc agagtttttg cagggcttgc
     9901 gcgagctgtg cgatcaacat caggcattat tggtgtttga tgaagtgcag tgcgggatgg
     9961 ggcggactgg cgatttgttt gcttacatgt actacggtgt gacgccggat attctgactt
    10021 ctgcgaaagc gttaggcggc ggcttcccga ttagcgccat gctgaccacg gcggaaattg
    10081 cttctgcgtt tcatcctggt tctcacggtt ccacctacgg cggtaatcct ctggcctgtg
    10141 cagtagcggg cgcggcgttt gatatcatca atacccctga agtgctggaa ggcattcagg
    10201 cgaaacgcca gcgttttgtt gaccatttgc agaagatcga tcagcagtac gatgtgttta
    10261 gcgatattcg cggtatgggg ctgttaattg gcgcggagct gaaaccacac tacaaaggtc
    10321 gggcgcgtga tttcctgtat gcgggtgcag aggttggcgt aatggtgctg aatgccggac
    10381 cggatgtgat gcgtttagca ccgtcgctgg tggtggaaga tgcggatatc gatgaaggga
    10441 tgcaacgttt cgcccacgcg gtggcgaagg tggttggggc gtaaataaga cgcatcaggc
    10501 aaaatatgtc ggatgcggcg tgaatgcctt atccgatggt ttagcctgta ttacgccttc
    10561 gaatcccgca acttgcgact cagccaaatc ccgtgatgcg gtcgctgacg ccatgccatc
    10621 gacgaaatgg tgtgcatggt gttcagatga ccaataaccc gctgtaaatg ctgctccagc
    10681 gtacctgccg cgccttcgtg cggctgcatc tccggcgcat ccatgatatt ggcatcgcca
    10741 gaactacccg gttcgtcata ctccagtcgc tgctgacaac gctgaatggc gatttcacaa
    10801 gactgtaaat actcttgtgc cagttcaggt ggcaatgccc ggtgttcccg cgccagcgtg
    10861 gtcatggcat taatatgctc aacaataaac tggctgtgcg ttacccacag tttcatatct
    10921 gccagataat ggctgttaaa cgccggttcc tgcatcgcct gattcaatga gttatacaga
    10981 gtgttatgtg cctgatttac ccgcattcgc tgccaggcca gtggcgtagg ttgcggatcc
    11041 tcgctaagaa tcaagcgaat cgcttcctga taggcttcta aagcgtcatg ggcgttttta
    11101 cgcaataacc cgctctgcca ctgcggccac agccagacag taccgccgaa agcaattaaa
    11161 caaccaataa tggtatcgat aagacgcgga aggatgtatt gctcgccgtt caaccacaat
    11221 agttgcaggg tatacactgc ggtaatagta aaaccgaccg tcgcccagcc gtagtttttg
    11281 cgcaatatca ggtagctggc gagggtggta atcagcatca acgtcagggt gtaaccttcg
    11341 ggaattttaa agtgcagcgc cacgcccgca atgattaacc cgaccacggt tcccacggag
    11401 cgattcacaa tcctcagacg ggttgcgcca tagccatttt gtgtcaccag caataccgtc
    11461 atcaggatcc agtacgactt cggcagatgc agcgcggtgc ccatcaggct ggcaacgctt
    11521 aacatcacac tgagtcgtcc ggcattgcgt agcgccggag actttagtga cagataactt
    11581 ttcagcgcag gaagtaatgg cattcgccgc tgtttatcgg ccagtaagtc acgggcatag
    11641 agcggttttt gggtgcgcag cacgcgggcg atgcggctga aatgccagta gcagaattgc
    11701 ccaaccggat tatccggatg ctggcgggcg attttttcca gtgcgccaat ttgcttttcc
    11761 atcgtaaaac gcgttggcag gcgatggtaa agaatgtcat cagccagcac gcgcaggcga
    11821 gcggcgacgg tttgcgcatt ccagcggata acttcttccg catggctacg ctcgaccagc
    11881 ttttgcacct cttccggctg atgcaaactg accgaaatat gttcctgtaa atccagcgcc
    11941 tcctggaaaa tacgcagcat ccgcttgtag tcagtattat tttgcgcgga aagcatatgc
    12001 atttgctgat agcactgggt aattagatcg accgcttttt gctggcgcac cagcagcggc
    12061 ggcagcgctt tttcagggtc ggtgtgctgg gtaagcaggc tgtatttggc ttcacaataa
    12121 tctgccagtt cacggtacag cagacttagt gactcgcgca gcggttgttc gcgccagatc
    12181 cagaaccaaa accagttaaa caatccgtac cagagagtgc ccaacgcata gatgagcaac
    12241 ggttcccaga ccggcatgta tcccgccaga ctgagggtaa aaatggctgc cagcagcgat
    12301 gcgggaagca attttgcgtg caatggcccc aactcagcag tgacgccaag taccagcgtt
    12361 aatccggtca gcaaaaaggg caggggaaca tcttttgcca gtagcagctg tgtcagtaag
    12421 ctacaggtgg caaacagcga cgcaccaatg attaagcgtt tgaaaaaacg tttatgaggc
    12481 gtatcaaggc ccgcaatatt gcaacaggca ggaacgagag agaagagcag accgaatcgt
    12541 aattcgccaa gcattaaccc aacggccacg ggcaaacata gcaccagcgt ttgtcgaagt
    12601 gcatagttga tatcggggtg ataaatcagt ctgcgccaca tcgggggaaa caaaatggcg
    12661 cgctaccagg taacgcgcca ctccgacggg attaacgagt gccgtaaacg acgatggttt
    12721 taccgtgtgc ggagatcagg ttctgatctt caagcatctt cagaatgcgt cccacggttt
    12781 cacgagaaca gccgacaatc tgaccgattt cctgacgggt aattttgatt tgcataccgt
    12841 ccgggtgagt catagcgtct ggttgttttg ccagattcag cagagtctgt gcaatgcggc
    12901 ccgtcacgtc gaggaacgcc aggttgccca ctttctctga agtgacttgc agacgacgcg
    12961 ccatctgtgc agacagacgc atcagaatgt ccgggtttac ctgaatcaat tggcgaaatt
    13021 ttttgtacga aatttcagcc acttcacagg cggttttcgc acgtacccat gcgctacgtt
    13081 cctggccctc ttcaaacagg cccagttcgc caataaaatc accctgattc agataggaga
    13141 ggatcatttc tttaccctct tcgtctttga tcagcactgc cacagagcct ttaacgatgt
    13201 agtacagcgt ttccgctttt tcaccctggt gaataagcgt gctcttggat gggtacttat
    13261 gaatgtggca atgagacaag aaccattcga gagtcgggtc tgtttgcggt ttgccaagca
    13321 ccatgcgcgg ttatcctctg ttataagctt tctccagagc cagataacgc cgctgtctct
    13381 ggattgccga aatatgcttc ccgctacctg ggaaggggct atcaactgta ctgcacggta
    13441 atgtgacgtc ctttgcatac atgcagtaca tcaatgtatt actgtagcat cctgactgtt
    13501 ttagcatagc tttcgctttg tgtctcctgg tgtctcgctt cagcatgacc caggtcgcct
    13561 tccgttgcgc gatttggtta gtacg
//
LOCUS       DAJTYD010000097        13426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTYD010000097 DAJTYD010000000
VERSION     DAJTYD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.097"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(170..517)
                     /gene="rplS"
                     /locus_tag="OO585_002575"
     CDS             complement(170..517)
                     /gene="rplS"
                     /locus_tag="OO585_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6630121.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(559..1326)
                     /gene="trmD"
                     /locus_tag="OO585_002576"
     CDS             complement(559..1326)
                     /gene="trmD"
                     /locus_tag="OO585_002576"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6630122.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(1357..1905)
                     /gene="rimM"
                     /locus_tag="OO585_002577"
     CDS             complement(1357..1905)
                     /gene="rimM"
                     /locus_tag="OO585_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6630123.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEDYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(1924..2172)
                     /gene="rpsP"
                     /locus_tag="OO585_002578"
     CDS             complement(1924..2172)
                     /gene="rpsP"
                     /locus_tag="OO585_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6630124.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(2309..3670)
                     /gene="ffh"
                     /locus_tag="OO585_002579"
     CDS             complement(2309..3670)
                     /gene="ffh"
                     /locus_tag="OO585_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6630125.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            3837..4628
                     /locus_tag="OO585_002580"
     CDS             3837..4628
                     /locus_tag="OO585_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6630126.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            4649..5935
                     /locus_tag="OO585_002581"
     CDS             4649..5935
                     /locus_tag="OO585_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6630127.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5990..6583)
                     /gene="grpE"
                     /locus_tag="OO585_002582"
     CDS             complement(5990..6583)
                     /gene="grpE"
                     /locus_tag="OO585_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6630128.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            6706..7584
                     /gene="nadK"
                     /locus_tag="OO585_002583"
     CDS             6706..7584
                     /gene="nadK"
                     /locus_tag="OO585_002583"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6630129.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7670..9331
                     /gene="recN"
                     /locus_tag="OO585_002584"
     CDS             7670..9331
                     /gene="recN"
                     /locus_tag="OO585_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6630130.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            9480..9821
                     /gene="bamE"
                     /locus_tag="OO585_002585"
     CDS             9480..9821
                     /gene="bamE"
                     /locus_tag="OO585_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6630131.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(9883..10173)
                     /locus_tag="OO585_002586"
     CDS             complement(9883..10173)
                     /locus_tag="OO585_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6630132.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10163..10600)
                     /gene="ratA"
                     /locus_tag="OO585_002587"
     CDS             complement(10163..10600)
                     /gene="ratA"
                     /locus_tag="OO585_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6630133.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            10771..11253
                     /gene="smpB"
                     /locus_tag="OO585_002588"
     CDS             10771..11253
                     /gene="smpB"
                     /locus_tag="OO585_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6630134.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            11468..11830
                     /gene="ssrA"
                     /locus_tag="OO585_002589"
     tmRNA           11468..11830
                     /gene="ssrA"
                     /locus_tag="OO585_002589"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11991..13426)
                     /locus_tag="OO585_002590"
                     /pseudo
     CDS             complement(11991..13426)
                     /locus_tag="OO585_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3278 a         3629 c         3176 g         3343 t
ORIGIN      
        1 ggtttaggaa aaaaatatcc catagccagt aacaagaccg cccagggccg cgccgagaac
       61 cgccgcgtcg cagtggtgat tacaacccct taaaacacga gcaaacagac aaaaaaaggg
      121 ccagccaatt ggccagccct tcttaacagg atgtcgctta agcgaaatct tagttaagac
      181 gctctttgat acgagcagcc ttaccagtac gctcacgcag gtagtacagt ttagctttac
      241 gaacagcacc acgacgtttg acagaaatgc tgtcaactac cggagagtga gtctggaaga
      301 cacgctcaac gccttcgccg ttggaaattt tacgaacagt gaatgcagag tgcagaccgc
      361 ggttacgaat agcgataacc acgccctcga atgcctgcag acgttttttg gaaccttcaa
      421 caacccatac tttcacttcc acggtatcac ccggacggaa ggaaggtacg tcctgcttca
      481 tctgctcttg ttcaagttgc ttaataatgt tgctcataat ttaatctctt atcctgggta
      541 aactgatatc tcgggggctt acgccatccc atcatgttta tgttgctgtt gtgcgtgttc
      601 cgttttgaac tccgccagca accttgcttg ctcttcagtc agagccaggt tttccagaag
      661 ttcaggtctt ctaagccagg tacggcccag cgactgtttc aaacgccagc gacgtatctc
      721 ggcatggttg cccgacagta acactggcgg aacttccatc ccttctaaca cctcaggccg
      781 cgtatagtgc gggcaatcca gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc
      841 atgtcccagt acccccggaa taaaccggga aacggagtca atcagcgtca ttgctggtaa
      901 ctcaccacca ctgagaacgt aatcgccgat tgaccattct tcgtcaattt cggtttggat
      961 cacgcgctca tctatacctt cgtagcgacc gcacaccaga atcaattttt gattcgttgc
     1021 caattcgctg acgcccgctt gatcaagctt gcgtccctgt ggtgacagat aaatcacctt
     1081 tgcgccttca cccgccgcgg cttttgctgc atgaatggcg tcccgcaagg gttgcaccat
     1141 cattaacatc cccggtccgc cgccgtaagg acgatcgtcc acggtacggt gccggtcatg
     1201 cgtgaagtcg cgaggactcc agctctggat gctcagcagg ccatttttaa ctgcccggcc
     1261 agttaccccg taatcggtaa ttgcgcggaa catttcagga aacaggctaa ttatgccaat
     1321 ccacatagcg ccgtctttta ccgtttatcc ggtggtttaa aaaccaggat cccaatctac
     1381 ttcgattgaa cgagtagtga gatcgacttt cttgataacc tgcccatcga ggaacggtac
     1441 gagacgttcc ttgataccaa acgcatcttt caggtttgcc ttaatgacga gaacgtcatt
     1501 agatccggtt tccatcatat cgacgacttt accgagatcg tagtcttcag tggttactac
     1561 ctggcagccc atcaggtctt tccagtagta gtcaccctct tcaagctgag gcaactgcga
     1621 tgaatccacg acaatttcac aattcgtcag caggttcgcc gcatcacgat cgtcaacgcc
     1681 tttcagcttg atgatcatgt cctgattgtg gtgcttccag ctttccagct ggacttgctg
     1741 ccactgaccc gccttctgga taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc
     1801 ggtggaagaa aacactctga gccacccacg aataccgtaa gacgaaccca tttttcccaa
     1861 aacgatggga tcaacaggtg cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac
     1921 agattaagct gctttgttta cttctttgat cagcgcagca acgcgatcag aaatagttgc
     1981 gccctggcca acccagtgag cgatgcgatc cagatccagg cgagtgcctt cttctttttc
     2041 gctagcgatt gggttgaaga aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg
     2101 gctgtcagcg acaacaacct ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg
     2161 aatagttacc ataacatcct cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga
     2221 gtccggtgtc atattaaaag cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc
     2281 agttaataac tctgtcgcat aaggtcattt agcgaccagg aaatcccggc ggcatcatcc
     2341 ccttcatgct tctcatcatc ttcgccattc cgcccttctt cattttcttc atcatgcgct
     2401 gcatgtcgtc gaactgtttc agaagacggt taacgtcctg cacctgcata ccgcaaccgg
     2461 cagcaatacg gcgtttacgc gaacctttga tgatttctgg cttagcgcgc tctttcatcg
     2521 tcatcgagtt gatgatggct tccatacgca ccagcacttt atcgtccatc tgtgacttga
     2581 cgttatccgg gatctgcccc atgcccggca gcttgcccat caggctggcc atgccgccca
     2641 tatttttcat ctggcgcagc tgctcaagaa agtcgttgag atcgaagccg tcaccttttt
     2701 tcagcttgct ggctaatttc tctgcctgcg cgcggtcaac tttgctttcg atatcttcga
     2761 tcagcgacag tacgtcgccc atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg
     2821 gctccagcgc ctcagttttc tcgccaacac cgaggaactt gatcggtttg ccagtgatgt
     2881 gacgaataga gagcgccgca ccgccgcggg catcgccgtc cactttggtc aacactacgc
     2941 cggtaagcgg taacgcttca ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca
     3001 tggcgtcaac cacaaacagg gtttcaaccg ggttaatcga cgcatggact tgtttgatct
     3061 cgtccatcat cgcttcgtca acgtgcagac gaccagcggt atccaccagc agcacgtcgt
     3121 agaatttcag tttggcttct ttcagcgccg cgttaacgat atctaccggc ttctgaccaa
     3181 catcagaagg gaagaaatca acgcccacct gctctgccag cgtctcaagc tgtttgattg
     3241 ccgccgggcg ataaacgtcg gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca
     3301 ggaacttacc gagtttacca acgctggttg ttttaccggc accttgcagg cccgccatca
     3361 gcacgaccgc aggcggttgc gccgccaggt tcagggtctg gttctcttcg cccatcgccg
     3421 caaccagttc gttacggacg attttgacga actcctgccc cggcgtcagg ctcttattaa
     3481 cttcatgacc aaccgctttc tcttttacgc gattgataaa ctcacgcact accggcagag
     3541 cgacatccgc ctccagcagc gccatgcgca cttcgcgcag cgtatctttt acgttgtctt
     3601 cagttaggcg tccacggcca ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat
     3661 tatcaaacat tgtctctcgc ctggggtgga aacggttggc cgcaatcgcg acacatcatc
     3721 agtattttgc cgcagtataa catgaaggcg tctttgttgt tatgcaacgg ttggagcagc
     3781 gttcacctga cgctatactg cttctctttc ttattgctca aactgttgac atcactatgc
     3841 ccgtttttgc tctgctcgcg cttgtcgcct actccgtcag tcttgcgctg attgttcccg
     3901 gtctgctgca aaaaaacggc ggctggcggc gcatggctat tatttctgcg gtcattgcgc
     3961 tggtctgcca cgcaatcgct ctggaagccc gcatcctgcc cgacggtgat agcggacaaa
     4021 acctcagcct gctgaacgtt ggttcattgg tcagtttgat gatctgtacg gtaatgacca
     4081 ttgtggcttc tcgcaatcgt ggctggttgc tgctacccat tgtctatgcc tttgcgctta
     4141 tcaacctggc gctggcgacc ttcatgccca atgaatacat cacccatctg gaagcaacgc
     4201 ctgggatgct ggtgcacatt ggcttatcgc tcttttccta tgccacgcta attatcgccg
     4261 ccctgtacgc gctgcaactg gcgtggattg attaccaact gaagaacaag aagctggcat
     4321 ttaaccagga aatgccgcca ttgatgagta tcgagcgtaa aatgttccac atcacgcaga
     4381 ttggcgtggt gctgctaacg ctcacgcttt gcactggcct gttctacatg cacaacctat
     4441 ttagcatgga aaatatcgac aaggctgtgc tctctatcgt ggcgtggttt gtctatattg
     4501 tgctgctgtg gggacattac catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg
     4561 ttgcgggcgc ggtaattctg acactggcct acttcggcag ccgaattgtc cagcagttaa
     4621 tcagctaaac ccagaaaagg agtttcccct ggaacacatt tctactacta cgttgatcat
     4681 tattctgatc atcatggtgg tcatttcagc ctatttttcc gggtccgaaa ccggaatgat
     4741 gaccctcaac cgctatcgtc tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg
     4801 cgtcgaaaaa ttgctgcgta agccagaccg cctgataagc ctggtgttaa tcggcaataa
     4861 cctggtcaat attcttgcct ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga
     4921 tgcgggcgtg gcaattgcga ctggtgtgct gacttttgtc gtactggtat ttgctgaggt
     4981 attgccgaaa accattgccg cgctgtaccc ggaaaaagtc gcttatccga gtagttttct
     5041 gctggctccg ctgcaaattt tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg
     5101 tatgctgatg cgcatgatgg gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa
     5161 agaagagttg cgcactatcg tgcacgaatc acgctcacaa atttcccgtc gcaatcagga
     5221 tatgctgctg tcggtgctcg atctggaaaa aatgaccgtt gatgacatca tggtgccgcg
     5281 cagtgaaatt atcggtattg atatcaacga tgactggaaa tcgattctgc gccaactctc
     5341 ccactcacct cacgggcgca tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat
     5401 gctgcgagta cgtgaagcct ggcgattgat gtcggagaaa aaagagttca ccaaagaaac
     5461 catgctgcgc gccgcggacg agatctattt tgtgccggaa ggtacgccgc tcagcacgca
     5521 gttggtaaag tttcagcgca acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga
     5581 cattcagggg ctggtgacgg ttgaagatat tctggaagag attgtcggcg atttcactac
     5641 gtcgatgtcg ccaacacttg ccgaagaggt cacgccgcaa aacgacggtt cggtgattat
     5701 cgatggcacc gccaacgtgc gggaaatcaa caaagccttt aactggcatc taccggaaga
     5761 tgatgcccgc acggttaatg gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg
     5821 cacccgcgtg cgtattggcg agtacgatat agatattctc gacgtacagg acaatatgat
     5881 taagcaggta aaagtttttc ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa
     5941 acggccctgc attcgcatgc agggccgtga attattacga aaacagaaat tacgcttttg
     6001 ctttcgctac agttaccatc gccgcacgaa tcgtacgacc attcagcgta taacccttct
     6061 gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct
     6121 gatgcacatt cgggtccagc ggcacgttag tttcggcgat cacttcaaca ccaaacttac
     6181 gcacaacatc cagcatcgac ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat
     6241 ccgggttagc tttatcagcc acttccagcg cacgatccag gctatcaatc accggcagca
     6301 attcgttgat gaatttctcc agcgcaaatt tgtgggcttt ttcaatatcc agttcagtac
     6361 gtcgacgcag gttttccatt tcggctttta cacgcaaaat gccgtcacgt tcacgggtct
     6421 gggcttcagc cagctgagct tcgagattcg caattttttc atcgcgcgga tccacctgct
     6481 cagcagaagc ttctggctca actgcctcaa tctcttcgtg ctgatccatg ataatttctt
     6541 ccggggcttg cccctcaggc gttttctgtt ctttactact catgaatttc tccgcgtttt
     6601 tttcgcattc atctcgctaa cttcgcttat tatggggatc agtttcaggg tttcaaggga
     6661 agcactcaca ttgtcatcaa tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc
     6721 aagtgtattg gcattgtggg acacccacgg caccccactg cactgacaac acatgaaatg
     6781 ctctaccgct ggctgtgcaa aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac
     6841 gaactgcaac tgaagaatgt gaaaactggc acgctcgcgg agattgggca actagctgat
     6901 ctcgcggtag tcgttggtgg cgacggtaat atgctgggcg cggcacgcac actcgcccgt
     6961 tacgatatta aagttattgg aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac
     7021 cccgataacg cccagcaaca gttagccgat gtactggaag gccactacat cagcgagaaa
     7081 cgttttttgc tggaagcgca agtctgtcag caagattgcc agaaacgcat cagcaccgcg
     7141 ataaatgaag tggtgcttca tccaggcaaa gtggcacata tgattgagtt cgaagtgtat
     7201 atcgacgaga tctttgcgtt ttctcagcga tctgatggac taattatttc gacgccaaca
     7261 ggctccaccg cctattccct ctctgcaggc ggtcctattc tgaccccctc tctggatgcg
     7321 attaccctgg tgcccatgtt cccgcatacg ttgtcagcac gaccactggt cataaacagc
     7381 agcagcacga tccgtctgcg tttttcgcat cgccgtaacg acctggaaat cagttgcgac
     7441 agccagatag cactgccgat tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac
     7501 catctgaatc tgattcatcc gaaagattac agttatttca acacattaag caccaagctc
     7561 ggctggtcaa aaaaattatt ctaattttac gccagcctct ttactgtata taaaaccagt
     7621 ttatactgta cgcaataaca gtaatggttt ttcatacagg aaaacgacta tgttggcaca
     7681 actgaccatc agcaactttg ctatcgttcg tgagcttgag attgattttc atagcggcat
     7741 gaccgtaata actggcgaga ccggcgcggg taaatctatt gcaatagatg ccctcggtct
     7801 ttgtctcggt ggtcgcgctg aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct
     7861 gtgcgcccgt ttttctctga aagatacgcc agcggctctg cgctggctgg aagaaaatca
     7921 gcttgaagac gggcatgaat gtttgcttcg tcgcgtaatc agcagcgatg gtcgctcccg
     7981 tggtttcatc aacggtacag ctgttcctct gtcacaactg cgcgaactgg gtcagttgct
     8041 gattcagatc catggtcagc acgctcatca attactcacc aaacctgagc accaaaaatt
     8101 cctgcttgat ggctatgcca atgaaacctc tctactgcag gaaatgaccg cacgttatca
     8161 gttgtggcat caaagctgcc gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc
     8221 cgcccgtgcg gaactgctgc aataccaatt aaaagaactt aacgaattta atccgcagcc
     8281 cggagagttt gaacaaatcg acgaagagta caaacgtctg gcgaacagcg gtcaattgct
     8341 gaccaccagc cagaatgcat tggcattaat ggcctacggt gaagacgcaa acctgcaaag
     8401 tcagctttac acggctaaac aactggtgag cgaattgatt ggcatggaca gcaaactgtc
     8461 cggcgtactt gatatgctgg aagaagctac catccagatt gctgaagcca gcgatgaact
     8521 gcgccactac tgcgatcgtc tggatctcga tcccaaccga ctatttgaac ttgaacagcg
     8581 catctcaaaa cagatttcgc tggcacgtaa acatcacgtc agccctgagg cattgccaca
     8641 gtattaccag tcgctactgg aagaacagca gcaactggac gatcaggccg actcacaaga
     8701 aacgcttgcg ctggcggtaa cgaaacatca tcagcaggca ctggaaatcg cgcgcgcatt
     8761 acaccaacaa cgccagcaat atgcagaaga acttgcacag ctgatcaccg acagtatgca
     8821 tgcgctctca atgccgcatg ggcagtttac gatcgatgtt aaatttgacg agcatcacct
     8881 gggcgctgac ggtgccgatc gtattgagtt tcgggtaacc accaacccag gtcagccaat
     8941 gcagcctatt gccaaagtcg catccggtgg tgaattgtcc cgcatcgcac tggcaaccca
     9001 ggtcatcacg gcgcgtaaaa tggaaacccc ggcactgatt tttgatgaag tggatgtagg
     9061 gattagcggt ccaacagcgg cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac
     9121 tcaggtgatg tgtgttaccc acctgccaca agtcgcggga tgtggtcatc aacactattt
     9181 tgtcagcaaa gaaaccgatg gtgcgatgac agaaacgcat atgcaatccc tgaataaaaa
     9241 agcgcggtta caagagctgg cgcgcctgct tggtggcagt gaagtcacac gtaatacact
     9301 ggcgaatgcg aaagaactgc ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa
     9361 acagcaaaac gccgtaagac cggaaagcaa aaggttttaa agtgatgaaa ggtctattat
     9421 catcggcata ttacagatga gccacgtact gctagggccc gaaaaggaat caaatcacta
     9481 tgcgctgtaa aacgctgact gctgcagcag cagtactatt gatgttgacc gcaggctgtt
     9541 ccactctgga gcgagtggtt taccgtcctg acatcaacca ggggaactat ctgaccgcta
     9601 acgacgtatc caaaatacgt gttggcatga cgcaacaaca agttgcgtac gcattgggta
     9661 caccgctgat gtccgatcca tttggtacga atacctggtt ctatgtcttc cgccagcaac
     9721 caggtcatga aggtgtaacg cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt
     9781 tgaccaatat tgataacaaa cctgcgctga gtggtaacta ataataaagt tgctctcaaa
     9841 gacgttaaaa aaggtgctca atgagcacct tttttctgtc tgttatttat tcgctgattt
     9901 ttctgctcgt tgcctgcgaa gctctttcgg atcggcaatg agagggcgat aaatctccac
     9961 ccgatcgcca tcatgcacac tatcgcttag ttttgccgga cggctgtaaa tgccgacttt
    10021 gtttttagtt aaatcgatat cggtacgcaa ttccagcaag ccactggcgc gaatagcttc
    10081 ttcaaccgtc gcgccctcct gcagcgtcac gcgctgcagg tactgcttct caggtagcgc
    10141 ataagccacc tcaacggcaa ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa
    10201 aagcctggac catattagac gccagctctt taaacacgcg accaaaggcg agttcaatca
    10261 acttattggt aaactcaaag tcgagatgaa actcgatacg acacgcctcc tggctcagcg
    10321 gcgtaaactt ccatccacca atcaatttct tgaacggccc atccaccaga ttcataagaa
    10381 tactttggtt actggtcaac tggttgcggg tagtaaacgt tttgctgatc ccagccttag
    10441 agacatctac cgccgcagtc atctgcccag gagtggactc cagaatccga cttccggtac
    10501 aacccggcaa aaactgagga taagactgaa cgtcattcac taactgatac atttgctccg
    10561 cgctgtaggg taccagtgcg gtccggctaa tctgaggcat aactatttcc atcaacaaaa
    10621 atccaacaaa taatatcatt tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa
    10681 catcgggttc atgctaagat agagccttgt cccccgcagg attgatatgg ggtgttttcg
    10741 atttcagatt accgatgatt cacgacgctt atgacgaaga aaaaagcaca taaacctggt
    10801 tcagcgacca tcgcgcttaa caagcgcgcc cgtcacgaat actttatcga agaagagttc
    10861 gaagcgggac ttgccctgca aggctgggaa gttaaatccc tgcgcgcagg aaaagccaat
    10921 atcagcgaca gctacgtcct tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc
    10981 acgccaatgg ccgtggcctc cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta
    11041 cttctcaacc agcgcgaact ggactcattg tacggtcgcg tcaatcgaga aggctatacc
    11101 gtagtggcgc tctccctgta ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc
    11161 aaaggtaaaa aacagcacga taaacgttca gatatcaaag aacgcgaatg gcaggtggat
    11221 aaagcacgta tcatgaaaaa cgcccaccgt taaacctgca ctccaattat tgaccagttc
    11281 ctcaccgcgc ctccctctcc ggcggcgcga atgaacatct tattggctat cacatccgac
    11341 acaaatgttg ccatcccatt gcttaatcga ataaaaatca ggctacatgg gtgctaaatc
    11401 tttaacgata acgccattga ggctggtcat ggcgctcata aatctggtat acttgccgtt
    11461 acacattggg gctgattctg gattcgacgg gatttgcgaa acccaaggtg catgccgagg
    11521 ggcggttggc ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta
    11581 gcagcttaat aacctgctta gagccctctc tccctagcct ccgctcttag gacggggatc
    11641 aagagaggtc aaacccaaaa gagatcgcgt ggaagccctg cctggggttg aagcgttaaa
    11701 acttaatcag gctagtttgt tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag
    11761 actgactaag catgtagtac cgaggacgta ggaatttcgg acgcgggttc aactcccgcc
    11821 agctccacca atcatgattg gacggtataa ggacaacacc aataaaaaca ggaggttagc
    11881 agtctcagca ggacaccgac cagacggtga agagacataa aaggatacgc aaaggagccg
    11941 cggctcctgg tgacatgaaa gcccacagat gtgggctttt tcgttgatgg tcagaacgac
    12001 cagttcacac cagctacccc gttccacggg gattccacac cggcaccatg gctatacccc
    12061 accccaagat gcccgcttaa cgtactgctg aatgaggctt taatacctgc ctggtatatt
    12121 ccacgtctgc ccgacaaatc attgacgaaa ttaccgtcac tattcacttt cacccggtta
    12181 tcatcgacaa attctttgcg cacagccgcc ttcagccacg gctcaacttc cataccgttc
    12241 cccagacgca tgttgtaact cagcgttgca cccagttcac gatataaacc tgcgggtatc
    12301 gactgatttc gattccatgc cattggataa atgatattca gggttatcag cggtgaaccc
    12361 ccgttaacga tgcatacggc gtcaggttcc agttaccatc ggtaaatcgc atcccggttt
    12421 caatgtgacc gcccagcccg ttgctgcgat aactgccgtt ggtggctcca ccgctgccaa
    12481 ccgtggagtc gcgagcttcg gtacctgcta ataccaataa attaccgcca ttttccagca
    12541 acatattggt cgctaaattg ccggaaatgg aaaaagtgcc gtactggtaa gtaccgctga
    12601 tttcaatacc gttagccgtg ctcgtctgga gagcggcacc gctgttctgg acgatatctg
    12661 tcgctttgcc attatcgtta actgtcagcg taccgccttc attgatcttt gtttttattg
    12721 cctctccgtt agctgaaact gtttgtattc cgccgtcgtt aatcgttgtc tcattcgcca
    12781 ccccctcgac gatttgttcg ccgccggtga gcgtcgtgcc tgtcgcagtg gcttttgttt
    12841 tgacgatctc ccgtccgccc gtattgacct gtgttttgtc agaagaggtg tctgactcca
    12901 cggttaacac gccgccattt gccagcagga tattgttcgc cgcaccctgc tcgatgctga
    12961 acgcgacgcc atccgcgcgt gttcctgtga cccgcgtcgc cctggtggtt gcaaccaaag
    13021 cgccctggct actctgctgt atccccgttg cgctgccttt ctcccgcaca tcgagtgtgc
    13081 cgccgtcatt aagcaccgag ttttcagcca gaccgccctc attaactacc tgtgaacccc
    13141 cattaataat ggaaccttcc gctgtcccgt ttgccataat ttgttgtagg ccagagacga
    13201 tatcggtatt gattgcctta ccataattct gaacggtttg cgtgccacca ttgatgtgtg
    13261 ttttctctgt tgacccacca tcaacaattt gttcaccact ttcgatattt gcctcagtgg
    13321 ctaatccata taccgtttgc ttgccacctt tgatatttgc tttatcagaa gtggcactgg
    13381 catatattgt ttgggtgcca gcactattga gtacagtgcc aacatc
//
LOCUS       DAJTYD010000098        13354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTYD010000098 DAJTYD010000000
VERSION     DAJTYD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.098"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            414..1616
                     /gene="proV"
                     /locus_tag="OO585_002591"
     CDS             414..1616
                     /gene="proV"
                     /locus_tag="OO585_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6630135.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1609..2673
                     /gene="proW"
                     /locus_tag="OO585_002592"
     CDS             1609..2673
                     /gene="proW"
                     /locus_tag="OO585_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6630136.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQVLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2731..3723
                     /gene="proX"
                     /locus_tag="OO585_002593"
     CDS             2731..3723
                     /gene="proX"
                     /locus_tag="OO585_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6630137.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAALK"
     gene            4015..5199
                     /locus_tag="OO585_002594"
     CDS             4015..5199
                     /locus_tag="OO585_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6630138.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRPRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5323..6060
                     /gene="ygaZ"
                     /locus_tag="OO585_002595"
     CDS             5323..6060
                     /gene="ygaZ"
                     /locus_tag="OO585_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6630139.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6050..6385
                     /gene="ygaH"
                     /locus_tag="OO585_002596"
     CDS             6050..6385
                     /gene="ygaH"
                     /locus_tag="OO585_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6630140.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6476..7006
                     /gene="emrR"
                     /locus_tag="OO585_002597"
     CDS             6476..7006
                     /gene="emrR"
                     /locus_tag="OO585_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6630141.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7133..8305
                     /gene="emrA"
                     /locus_tag="OO585_002598"
     CDS             7133..8305
                     /gene="emrA"
                     /locus_tag="OO585_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6630142.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTVREISLAPVNKLIDDIVKANAG"
     gene            8322..9860
                     /gene="emrB"
                     /locus_tag="OO585_002599"
     CDS             8322..9860
                     /gene="emrB"
                     /locus_tag="OO585_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6630143.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="OO585_002600"
     CDS             complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="OO585_002600"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6630144.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="OO585_002601"
     CDS             complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="OO585_002601"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6630145.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12218..12646)
                     /locus_tag="OO585_002602"
     CDS             complement(12218..12646)
                     /locus_tag="OO585_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6630146.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12643..13209)
                     /gene="yqaB"
                     /locus_tag="OO585_002603"
     CDS             complement(12643..13209)
                     /gene="yqaB"
                     /locus_tag="OO585_002603"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6630147.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLAPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         3114 a         3490 c         3403 g         3347 t
ORIGIN      
        1 gctcctctta tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag
       61 ggtgttattt tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt
      121 tttatcacgc aaatattctg tagtgatcta cactgagact ctgttgcatt attcgcctga
      181 aactacaata ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat
      241 ttgctctcag cccttatatc acgggaaatt ccggcgattt gctcgcatca atattcatgc
      301 cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact
      361 atttccattg ggtaatatat cgacataaac aaataaagga atctttctat tgcatggcaa
      421 ttaaattaga aattaaaaat ctttataaaa tatttggcga gcatccacag cgagcgttca
      481 aatatatcga acaaggactt tcgaaagaac aaattctgga aaaaactggg ctatcgcttg
      541 gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat
      601 ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg
      661 ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg
      721 tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg
      781 tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg
      841 aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg
      901 atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc
      961 cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga
     1021 tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc
     1081 acgatctcga tgaagctatg cgtattggcg accgaattgc cattatgcaa aatggtgaag
     1141 tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta
     1201 ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga
     1261 caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat
     1321 tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg
     1381 cagtttccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc
     1441 tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg
     1501 tcggacaggc accctgtgcg gtacccgtgg tcgacgagga ccaacagtat gtcggcatca
     1561 tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa
     1621 aataatccgt gggataccac gccagcggcg gacagtgccg tgcaatccgc agacgcctgg
     1681 ggtacaccgg cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct
     1741 gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc
     1801 gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag
     1861 ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc agcaagtgct gctgggtatg
     1921 cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga
     1981 atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag
     2041 gccatggtta ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt cattggtttg
     2101 ccgctgggga tctggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt
     2161 gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt
     2221 atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc gattatccgt
     2281 ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc
     2341 ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgagcatt
     2401 atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg
     2461 attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg
     2521 cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg
     2581 caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct
     2641 gttggtctgc tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc
     2701 attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg
     2761 tttgccacgc ttatctctac acaaactttt gctgccgatc tgccgggcaa aggtattact
     2821 gttaatccag ttcaaagcac catcaccgaa gaaaccttcc agacgctgct ggtcagccgt
     2881 gcgctggaga aattaggcta taccgtcaac aaacccagcg aagtagatta caacgttggc
     2941 tacaccttgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat
     3001 gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt
     3061 aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc
     3121 aacatcgcac aactgaaaga gccgaagatc gccaaactgt tcgataccaa cggcgacggt
     3181 aaagcggatt taaccggttg taatcctggc tggggctgcg aaggtgcgat caaccaccag
     3241 cttgccgcgt atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg
     3301 atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg
     3361 ccgtactggg tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg
     3421 ttctccgcac tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac
     3481 ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctggaccga gaaaaatccg
     3541 gcggcagcca aactgtttgc cattatgcag ttgccagtgg ccgatattaa cgcccagaac
     3601 gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg
     3661 atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgctgaag
     3721 taatttttat tcaggcggat aaggcgtttt tgccttatcc gccgctctgt acacaatgcc
     3781 tgatgcgacg ctgacgcgtc ttatcatgcc tacaaaatac actcattccg tatggcggat
     3841 aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc
     3901 ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc ccatattcat
     3961 caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa aataatgact
     4021 aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc caccggtctg
     4081 gcggtagcca gtaactatta cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc
     4141 ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta tgcagcaggt
     4201 ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg
     4261 accttactgg cggcaggcgg catgttaatc actgccagca gtcagtcgct ggcgatgatg
     4321 atcctcggta cggcattaac cggtttattc tctgtcgtgg cacaaattct ggttccgctg
     4381 gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt
     4441 ctgctgttgg ggatcttact cgcacggacc gttgccggat tgctggcgaa tctcggcggc
     4501 tggcgcaccg tcttttgggt tgcctcggtg ttaatggcac tgatggcgct ggcattatgg
     4561 cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt gttaggttcc
     4621 gttttcagca tgtttatcag cgataagatc ctgcgccccc gcgcattgct gggctgcctg
     4681 acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc cgctccacct
     4741 tttaactaca gcgatggtgt cattggtctg tttggacttg cgggagctgc cggagcgttg
     4801 ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat cgcaccacac cacaactttc
     4861 ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac ttccgtactg
     4921 gcgctgatta tcggcatcct ggtgctggat ctcaccgtac agggcgtgca tatcactaac
     4981 cagacggtaa tttatcgaat acaccctgat gcgcgtaatc gcctgaccgc aggttacatg
     5041 accagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc ctggcaacat
     5101 ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa cttactggtc
     5161 tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt
     5221 ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta agatattcat
     5281 tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag ccctactcca
     5341 cagcctgctc ttggttcggc gaccttcatg gaaggatgca aagacagttt accgattgtt
     5401 attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct gggattctct
     5461 cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt
     5521 accgcgatgc tggcagccgg gagtagtttg tgggttgctg cactgaccgt catggcaatg
     5581 gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa
     5641 aaatcgaaaa ccgccctgtg ggcatttggc ctgacggatg aggtttttgc cgccgcaacc
     5701 gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg cattgccttc
     5761 agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg cagcggcttg
     5821 ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc actctttatg
     5881 agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt
     5941 ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc aggcattgtc
     6001 tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga tgagctatga
     6061 ggttctgctg cttgggttac tggttggcgt ggcgaattat tgcttccgct atttgccgct
     6121 gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta ttttgctcga
     6181 caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac cagaagtgat
     6241 gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg gtgccagttt
     6301 ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct atgggctcgc
     6361 ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac aatacattta
     6421 ctttatttgt tactgtcgtt actatatcgg ctgaaattaa tgaggtcata cccaaatgga
     6481 tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc acgaagattt
     6541 tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca agctgctgga
     6601 gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta tggcgttgat
     6661 tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt gtgctcttgg
     6721 atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga
     6781 acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg aaaaaggtca
     6841 cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac tctggtccgc
     6901 gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc tctcccgtct
     6961 cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg tcatctcgct
     7021 caaaaatcca gatttataaa agaaaaaatg accggccagc atcgcaacat gctggccttt
     7081 ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca atatgagcgc
     7141 aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct
     7201 gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga tttattggtt
     7261 tttggtactg cgtcatttcg aagaaaccga tgacgcatac gtggcaggga atcagattca
     7321 aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca ccgattttgt
     7381 aaaagaaggc gatgttctgg tcactctcga cccgacagat gctcgccagg cgtttgaaaa
     7441 agccaaaact gcactggctt ccagcgttcg ccaaacccac cagctgatga ttaacagcaa
     7501 acagttacag gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta
     7561 caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc
     7621 ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gctattcaac aatacaatgc
     7681 caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc aacaggctgc
     7741 caccgaagta cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca gtccgatgac
     7801 cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa cgacgccgct
     7861 gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag agacgcagat
     7921 tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg gcgatgatgt
     7981 gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat tctcactgct
     8041 tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat
     8101 cgaactggac cagaaacagc tggagcaata cccgctgcgc attggtttgt ccacgctggt
     8161 gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac gttcaacacc
     8221 ggtcgcggta agtaccgtgc gtgaaatcag cctggcacct gtcaataaac tgatcgacga
     8281 tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc
     8341 tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca ttcatgcagg
     8401 tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat ctgggctcat
     8461 cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc
     8521 cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt tggtccacca
     8581 tcgcctttgc tattgcgtcg tgggagtgtg gtgtctccag cagcctgaat atgctgatct
     8641 tcttccgtgt gattcagggg attgtcgctg ggccgttgat cccactttcg caaagtcttt
     8701 tgctgaataa ttacccgcca gccaaacgct caatcgcgct ggcgttgtgg tcgatgacgg
     8761 tgattgtcgc gccaatttgc ggcccaatcc tcggcggtta tatcagcgat aattaccact
     8821 ggggctggat attcttcatc aacgtgccaa ttggcgtggc ggtggtgttg atgacactgc
     8881 aaaccctgcg cggacgcgaa acccgcaccg aacggcggcg gattgatgcc gtggggctgg
     8941 cactgctggt tattggtatc ggtagcctgc agattatgct cgaccgcggt aaagagctgg
     9001 actggttttc atcacaggaa attatcattc ttaccgtggt ggcggtggtg gctatctgct
     9061 tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg ttgtttaagt
     9121 cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg
     9181 cgattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg acctgggcag
     9241 gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt atcggccgct
     9301 tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg tatgccgtct
     9361 gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg tcggcctggc
     9421 cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc accattacgc
     9481 tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctctaac tttacgcgaa
     9541 cgctggcggg gtctatcggc acgtcgataa ccacgaccat gtggaccaac cgcgagtcga
     9601 tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt
     9661 acagtcaact ggaagggctt gggatgacgc aacagcaggc atcaggctgg attgcccagc
     9721 agatcactaa tcaggggctg attatttccg ccaatgagat cttctggatg tcagccggga
     9781 tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc gccctttggc gcaggtggcg
     9841 gcggaggtgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc ttttttcaat
     9901 taattgtgaa gatagtttac tgactaaatg tgcagttcct gcaacttctc tttcggcagt
     9961 gccagttctt cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg cgcaatatcc
    10021 tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt cagctccggg
    10081 atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca ggcatcagca
    10141 acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg gcagcccatt
    10201 ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat aaaaccagca
    10261 aacaggtgct ccagagtatg gctccctctt tctggcatca cttctttgtt cggcacgcag
    10321 aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc
    10381 cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa caacggcatt
    10441 tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca ggcgagtctg
    10501 agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg aaattttggc
    10561 cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac accgcaaacg
    10621 gttcggtatc agcggtttcc atttcctgct gacggcgttc agatgcctcg cgctcggcta
    10681 caaaatcctc ttcgcgcaga atttccaacg gctcttcacg cagcagatta cggtaggctt
    10741 ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat catagacctt aagatacggg
    10801 cagagaaagt cagatcggga ttatcgaagc aggcgaccag ttcatcacac actttctgat
    10861 acgctttgcc gccgttaatg ctatccaacg tttgcgcgac gcgtttcaga tcgcggaaca
    10921 gatctttacc cacctgcggt aacgggaact gcgcggtttc gcagccgata cccagcgtca
    10981 ggcccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta caggcaagtt
    11041 cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac aggtcgagga
    11101 atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca
    11161 cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg
    11221 taacgcgttt tggacgaatc ggcgcataca gttcgttttc aatctgcagc acgttgctat
    11281 tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct tccgatggcg
    11341 ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag gtaataccaa
    11401 gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag gtcgcatacg
    11461 gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt tttccttgca
    11521 ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc caaccgaaac
    11581 gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg gcatcagtgc
    11641 ccgagatatc accgcacttc gcttgccaga atgccattgg caaagagaaa ttgtagtgca
    11701 cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca cgatacagcg
    11761 ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg
    11821 cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg cgcgccgtat
    11881 aacgatgcag atcgcgcata aaggtcaaca tatgttcaat atcaccatcc actggtgtaa
    11941 tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac
    12001 ctaatgcttc aggatgacct gttgttgcct gtgtgccatc agcattaaca cgcaaagttt
    12061 cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc agccaggcca
    12121 gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg ttttaattag
    12181 cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac atcatgccct
    12241 gaacggtagc tgctgcaact gcaacatagc gtaaagcttt accaaggcac aaaaaaaaga
    12301 ttaccggtcc ccacgagatg cgcatccatc ccgctaacag acacagtaaa tcgccaacca
    12361 cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag ccagtagctt
    12421 tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata acgttagtta
    12481 accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga taactgatcc
    12541 cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt gtagcgctga
    12601 gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca agcgaacatc
    12661 cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat cttcaaagac
    12721 cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc
    12781 gggtttatgg tgtttgacgt gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc
    12841 caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta cagccattgg
    12901 gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga cactatccag
    12961 cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggg cgagatcggc
    13021 ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat taagcgcaat
    13081 cattgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc acgctttacg
    13141 gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac ctgcataacg
    13201 ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt aattgcttga
    13261 ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataaagta gttatttaga
    13321 aatgagatat ttttgaagga aatttttggg agaa
//
LOCUS       DAJTYD010000099        13313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTYD010000099 DAJTYD010000000
VERSION     DAJTYD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.099"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..309
                     /locus_tag="OO585_002604"
     CDS             <1..309
                     /locus_tag="OO585_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6630148.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OO585_002605"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OO585_002605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6630149.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO585_002606"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO585_002606"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6630150.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3391
                     /gene="ilvY"
                     /locus_tag="OO585_002607"
     CDS             2501..3391
                     /gene="ilvY"
                     /locus_tag="OO585_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6630151.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="OO585_002608"
     CDS             complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="OO585_002608"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6630152.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="OO585_002609"
     CDS             complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="OO585_002609"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6630153.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="OO585_002610"
     CDS             complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="OO585_002610"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6630154.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="OO585_002611"
     CDS             complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="OO585_002611"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6630155.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8059..9705)
                     /locus_tag="OO585_002612"
     CDS             complement(8059..9705)
                     /locus_tag="OO585_002612"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6630156.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9708..9758)
                     /locus_tag="OO585_002613"
     CDS             complement(9708..9758)
                     /locus_tag="OO585_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630157.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="OO585_002614"
     CDS             complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="OO585_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6630158.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10295..11815
                     /locus_tag="OO585_002615"
                     /pseudo
     CDS             10295..11815
                     /locus_tag="OO585_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="OO585_002616"
     CDS             complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="OO585_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6630159.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12297..13136
                     /gene="hdfR"
                     /locus_tag="OO585_002617"
     CDS             12297..13136
                     /gene="hdfR"
                     /locus_tag="OO585_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6630160.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13232..13307)
                     /locus_tag="OO585_002618"
     tRNA            complement(13232..13307)
                     /locus_tag="OO585_002618"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13272..13274),aa:Trp,seq:cca)
BASE COUNT         3093 a         3600 c         3448 g         3172 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 ggcctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc
      181 agtcgaaacc gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catattgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg ttcgataaag ttgtgttctc ttgtccggta
      721 ctggagccga ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag
     1081 aacagatagt taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac
     1201 tcttcaatga tgccggaatc gaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca taagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgca cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acgtcggagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca aatccgcctg cgggatcagt tcttcgtaag tgcccacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga tgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacattgag ggctgctcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtgacgct gactgaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca gttgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 accgattctc gaccgcttcc gcgcggaaca cccgtcggtg gagatcaaac taaccactgg
     2881 tgatgcggca gatgcgatgg aaaaggtagt cactggcgaa gcggatctgg cgattgccgg
     2941 gaaaccggag actctgcccg gcgcagtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcg cccgcgctgc cctgcccggt gcgtaaccag gtttcgtcag agaagccgga
     3061 ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg aagtagtgct
     3241 ggaaaacagc cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgaaaa
     3301 aacgccgttt gaactgggcg tttgtgcgca gaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aagattttgc cgaaccatta acccgccaaa aagaacctga acgccggatt
     3421 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3481 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3541 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3601 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3661 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3721 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3781 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3841 gaccgctcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3901 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     3961 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat
     4021 gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc
     4081 caccgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4141 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt
     4201 taccgctacg ccttcagcaa atagccctac gcgcggcaga tcaaccggat gacccgcatc
     4261 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4321 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4381 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4441 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4501 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4561 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4621 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4681 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4741 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4801 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgctg cctcgtaaac
     4861 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccag acaggggttg
     4921 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     4981 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5041 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5101 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5161 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5221 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5281 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5341 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5401 accgcgtcgt cctggctttc gtacactttt gccgggccgg tgaatttgag gatgctgtca
     5461 tcgacgcccg cggttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5521 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5581 tcgtccagcg tatcccaacg gcaatcttgc gagaatgcct gggtggtacg gatacccgca
     5641 gggccggcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgc
     5701 tccagcgttt gcggcaacgt caggccgagc acgtttttca catcacggtt cagtaacccc
     5761 gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac atcttccata
     5821 tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg ggaaagctta
     5881 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     5941 acggtgttag tcgatccacc catcgcgata tccagcgtca tggcgttttc aaacgccgcc
     6001 ttactggcga tattacgcgg cagtgcactt tcgtcgtttt gctcgtaata acgtttggtc
     6061 aattcaacaa tgcgtttacc agcattaagg aacagctgct tacggtccgc gtgggttgcc
     6121 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6181 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6241 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6301 agtttgatta tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
     6361 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6421 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6481 tcagcgatca gttcgcgaga tggaagtgaa taaagcatcc ccccgtggcc catggcaatc
     6541 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6601 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6661 gagttcacaa ccgcgataat cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc
     6721 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6781 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
     6841 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6901 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc
     6961 aacctgaata ccgtctacgc tgcgcactgg cgtgatttct gccgcagtac cggacataaa
     7021 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7081 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7141 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7201 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7261 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7321 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7381 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7441 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7501 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7561 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7621 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7681 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7741 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7801 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7861 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7921 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     7981 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
     8041 cattgacctg atattgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8101 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8161 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8221 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8281 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8341 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8401 cacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt tcggtcgcgc
     8461 gacttgtgcg ccaaccgccg ctggtaaacc aaaacccata gtgcctaagc cgctggaggt
     8521 gatgaaattt tccgggcgag tgtgggcgat gtgttgcgca gcccacatct ggtgctgccc
     8581 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8641 cggcgcgtag atagcgtcgc cgggatggtc gtaacgccag gcatgttcat cacgcagctg
     8701 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaattctg gtaacagagc
     8761 atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8821 gatatccata tggataacgc tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
     8881 gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc
     8941 cgctttggtg ccatgcattc ccagcatgcc cagatagtac ggataatctg cttcaactgc
     9001 gcccagccct ttcagcgtgc aggtggcagg catttttgtg gtagcgagaa attctcgtaa
     9061 agcaggaact gcctgcgcca tacccacgcc accaccaacg tacagcatcg gtttttgcgc
     9121 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9181 aacggtggtg aaccacggtt ccaggtcacc gctggctagc tggatatctt ttgggatatc
     9241 gaccagaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccataatgcg
     9301 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9361 caggacatcc acttcctgaa atgcatcagt gccgataaac ggtgcggaca cttgaccggt
     9421 gatggcaaca acagggatag aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9481 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9541 gatagccgcc attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc
     9601 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9661 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtg
     9721 ctgaatgttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9781 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9841 atttctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9901 cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
     9961 cttgttcgaa ggaatgccta aagagttacc ataggttttg taaatagtac aagatatttt
    10021 ttggccgatt tttatgatgc gtttaataat ttcaatataa gttgttgttt tttaaaaata
    10081 aaatagtgag taaaaaaatt caatttcagt atgaatgttg ttcccttcct cgtagttcat
    10141 cctttgtccg ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt
    10201 gcacaaataa acaggaatca gccactaaaa ttcacgaatt agcgggctac agcagcgata
    10261 tcttatgcac ataatggtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg
    10321 cgcagccctg ggagtaaatg cgcccccgat cactgttgag gtacatatca gtaaaggtct
    10381 acccggctta acgaaggtgg gcttaccaga aacaacggta aaagaagctc gcgatcgcgt
    10441 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10501 ggctccagct gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10561 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatggat tagtcggaga
    10621 actggcgctt acaggcgctc tgcgtggcgt tcccggcgca atctccagta caactgaagc
    10681 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10741 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10801 taagcacgct ctcgaacgcc cgaaaccaac tgatacagta tcccgggcgc tacaacatga
    10861 tctcagtgat gttatcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10921 cgggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    10981 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagtg ctgcgatatt
    11041 aagtctggta aacgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    11101 tcatcacagt gcatcgttaa ctgcgatggt aggcggtggc gcaattccag ggcccgatga
    11161 aatttcgctg gcgcataacg gcgtgctttt tcttgatgag ttacctgaat ttgaaaggcg
    11221 tacactggat gccttgcgag agccgattga atccgggcag atccatcttt cacgcacgcg
    11281 agcaaaaata acctatccag cccgtttcca gcttgtcgcg gcgatgaatc ccagccctac
    11341 cggacattat cagggaaacc ataaccgctg cacaccagag caaacgctgc gttatctcaa
    11401 ccgactctct ggtccctttc tcgaccgctt cgatctctca ctggagatcc cattaccgcc
    11461 tcccggtatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    11521 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaacg cctggctgga
    11581 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggctggaaga
    11641 aacgctgatc catctggggt tatcgattcg tgcctagcag cggttattga aagttgcacg
    11701 aaccattgct gatattgatc agtctgacat tatcacacgt caacatttgc aggaggcagt
    11761 tagctatcga gcgattgacc gtttgctcat ccatctgcag aaactactga cataaaaaaa
    11821 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    11881 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    11941 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    12001 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    12061 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    12121 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    12181 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    12241 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    12301 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    12361 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    12421 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    12481 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    12541 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat
    12601 gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca ggatgcccat acaggcttac
    12661 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    12721 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    12781 gatatttcac tttagcgctt tataccagtg ccccttcaaa actaaaggga gatcttaatt
    12841 atctgcgact tgagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    12901 acgaagtgcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    12961 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    13021 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    13081 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatatg
    13141 aatggctggc aaggatgccg gtagaaggat ttacttcgga gagggttatt tcagataaaa
    13201 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    13261 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tag
//
LOCUS       DAJTYD010000100        13277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTYD010000100 DAJTYD010000000
VERSION     DAJTYD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.100"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..187
                     /locus_tag="OO585_002619"
     CDS             <1..187
                     /locus_tag="OO585_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6630161.1"
                     /translation="CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELL
                     NRRLSESVPCMPHKKAG"
     gene            208..909
                     /gene="mlrA"
                     /locus_tag="OO585_002620"
     CDS             208..909
                     /gene="mlrA"
                     /locus_tag="OO585_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6630162.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDDGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            969..1076
                     /locus_tag="OO585_002621"
     CDS             969..1076
                     /locus_tag="OO585_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6630163.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1057..1788)
                     /gene="yehW"
                     /locus_tag="OO585_002622"
     CDS             complement(1057..1788)
                     /gene="yehW"
                     /locus_tag="OO585_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6630164.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1793..2719)
                     /gene="yehX"
                     /locus_tag="OO585_002623"
     CDS             complement(1793..2719)
                     /gene="yehX"
                     /locus_tag="OO585_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6630165.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2712..3869)
                     /gene="yehY"
                     /locus_tag="OO585_002624"
     CDS             complement(2712..3869)
                     /gene="yehY"
                     /locus_tag="OO585_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6630166.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3876..4793)
                     /gene="osmF"
                     /locus_tag="OO585_002625"
     CDS             complement(3876..4793)
                     /gene="osmF"
                     /locus_tag="OO585_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6630167.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5045..7342)
                     /gene="bglX"
                     /locus_tag="OO585_002626"
     CDS             complement(5045..7342)
                     /gene="bglX"
                     /locus_tag="OO585_002626"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6630168.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGSKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7538..9253
                     /gene="dld"
                     /locus_tag="OO585_002627"
     CDS             7538..9253
                     /gene="dld"
                     /locus_tag="OO585_002627"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6630169.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9292..10224)
                     /gene="pbpG"
                     /locus_tag="OO585_002628"
     CDS             complement(9292..10224)
                     /gene="pbpG"
                     /locus_tag="OO585_002628"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6630170.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10398..10985)
                     /locus_tag="OO585_002629"
     CDS             complement(10398..10985)
                     /locus_tag="OO585_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6630171.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11155..11733
                     /locus_tag="OO585_002630"
     CDS             11155..11733
                     /locus_tag="OO585_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6630172.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11863..12624)
                     /locus_tag="OO585_002631"
     CDS             complement(11863..12624)
                     /locus_tag="OO585_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6630173.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12677..>13277)
                     /locus_tag="OO585_002632"
     CDS             complement(12677..>13277)
                     /locus_tag="OO585_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCR6630174.1"
                     /translation="PKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFY
                     PDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSI
                     ASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDTLRVVGLAQARFNAGIIAGSR
                     VSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         3194 a         3720 c         3357 g         3006 t
ORIGIN      
        1 atgctggtca cttgccgcag gagctaatcc tagttttatc gcaagccaga tgggccacac
       61 aaacgcacaa atggtattca atgtttacgg agcatggatg aaagacaaca atcacgaaca
      121 gatagaactt cttaacagaa gactatctga aagtgtccca tgcatgcccc ataagaaagc
      181 ggggtaaaat aaaaacttgc aaaatcaatt ggtttacctt taatccctgt cacgttacgc
      241 gcgtggcaga ggcgttacgg gttgctgaaa ccgcaacgga cagacgacgg tcatcgactg
      301 ttcaacgatg ccgatattga ccggatccgc gagatcaaac gctggatcga caacggcgtg
      361 caggtcagca aagttaaaat gctgctcagt aatgaaaatg ttgatgtgca gaacggctgg
      421 cgcgatcagc aagaaacatt actgacctac ctgcaaagcg gcaatctgca tagcctgcga
      481 acgtggatca aagagcgcgg tcaggattac cccgcccaga cactcaccac acatctgttt
      541 attcctctgc gccgacggct tcagtgccaa caaccgactc tccaggcgct gctggcgatc
      601 ctcgacggcg tactgatcaa ctacatcgcc atttgtctgg cttcggcacg tagaaaacag
      661 ggtaaagatg cgctggtggt tggctggaat attcaggata ccacccgtct gtggctggag
      721 ggctggattg ccagtcaaca aggatggcgc attgatgtcc tcgcccactc gctcaatcaa
      781 ctacgccctg aactattcga aggccgtaca ttgctggtgt ggtgcggtga aaatcgaacc
      841 tccgcccaac agcagcaact caccagttgg caagaacaag gccatgatat tttcccactc
      901 ggcatttaat gattcgttaa caaatgcgct ttactgtaca atcctttcgt taacataagg
      961 agtgcaatat gcgcatagct aaaattgggg tcatcgccct gttcctgttt atggcgttag
     1021 gcggaattgg tggcgtcatg ctcgcaggtt atacctttat tttgcgtgct ggctaagcgc
     1081 ctgcaccagc ctttcaaaca ggcggtctgc aatgatcgcc gccagtgcca ccagtaacgc
     1141 cccctggatc acatacgcgg tattaaatcc gctaagcccg atgatgatgg gcgtacccag
     1201 cgtgctggcc cctaccgttg aggcgatcgt cgccgtacca atgttgataa tcaccgaagt
     1261 tcgcacgccc gccagaatca ccggagccgc cagcggtagc tcgaccttac gcagtcgctg
     1321 accacgactc attcccatac ctttcgcaac ttctgtcacg ctggcatcaa tcgctcccag
     1381 cccggcaagt gtcgcctgca ggacgggcag cacaccgtaa aggatcaagg cgataatcgc
     1441 tggttgcaga ccaaagccga tcaccggaac ggcaatcgcc agcactgcga caggcggaaa
     1501 agtctgtcca acggcggcaa tagtttccac cagtgggcga aattccgcgc cccacgggcg
     1561 agtgacagca attccggcac cagtgccaat gatcaccaca aacaaactcg aaattcccac
     1621 cagccagaaa tgagccagtg ccagagctgc aaaactttct tgctgataaa cgggtcgtgg
     1681 cagttgtggg aacaaggcag caaacagcgg ctggctgtaa ggcagccaga aaatcagtgc
     1741 cacaaacaaa gcaatgagcc agaacagcgg atcgcgcaac atcttcatac gcttacgcct
     1801 cctccagcag atcctgaaaa tgcagcgtgc cgcaaggctg gccctgcgtg ttcaccaccg
     1861 gcagcacctc gcatccccgc gcgacaaaca gggagagcgc atcgcgtagc gtcatctctt
     1921 ctgccagtgc ctcaccttct gcccgttctt cgcgacacac gtaatccgcc acactacgta
     1981 acgaaagcag gcgcacaccc agttcgctgc gcccaaaaaa ctggcggaca aaatcgtttg
     2041 ccggacgagt cagcatcgtc agcggattgc cctgctgcac cacttcaccg tgatccatca
     2101 ataccagatg ttctgccagc cgtagcgcct catcaatatc atgagtgacc agcacaatgg
     2161 tacgccccag caaacggtga atgcgcgtca tctcttgttg caacgcgccg cgcgttaccg
     2221 ggtccagtgc gccaaaaggt tcatccatca gtaagacttg cggatcggca gccagtgcgc
     2281 gcgccactcc cacacgttgc tgctgaccac cggaaagctg atgcggataa cgctcacgta
     2341 aatttgactc cagccccagt agcgccatta attcgtcgat acgatcgtca atccgcgccc
     2401 gcgaccattt ttgtaattgc ggcacggtag cgatgttttg cgccacgctc cagtggggga
     2461 acaggccaat agattgaata gcatagccca tccggcggcg caactccagt actggcagcg
     2521 agcgaatttc ttctccggca aagcggatca ctccgctgtc atgctccacc aggcggttaa
     2581 tcattttcag ggtggtggat ttgccggagc cagatgtgcc aatcagcacc gaaaaactcc
     2641 cttcctgaaa attgagattg agatcgttaa cggctttttg tgcgccgaac agtttgctga
     2701 catggctaaa ttcaatcatt acgtttcacc ttcagcagtg cgataagcaa atcgaacagc
     2761 gcgtcggtaa gcaccgccag aacaattacc gggatcaccc ccagcaacac taaatcaatg
     2821 gcgctgctta gcagcccctg gaaaaccagc gcaccaaaac cgcctgcgcc gattaacgcc
     2881 gcaatcaccg ccatacctac agtttgcacc atcaccaccc gcaggctgcg cagaaatacc
     2941 ggtaacgcca gtggtaactg aacatgcagg aatcgctgcg ccccgctcat gcccatcgct
     3001 ctggcactct ccagcacatc gcgcgggatc tggttcaaac cgactaccac gccgcgcacc
     3061 agcggcagca aggcatagag caccagcgca atcagtgcgg gtgtcattcc ggttcctgct
     3121 atgccgagct tccccagcca cggaaaggcc gtcaccagcg cggcaagcgg cgcaatcaac
     3181 aggccaaaga gcgccaccga aggcacggtc tgaatgacat tgagcagaga aaaaattgcc
     3241 ccctgccgag cagtggaaaa gtagcaccag atgcccaacg gcacaccaat cactaacgca
     3301 ggcagcaccg caccaaacag caacgtcaga tgttgtgcca gcgcgtcgtc aaacacatcc
     3361 tgacggttgg cgtattcttt cattagtgag agatcgttaa gcgtgccgga gtacagcaac
     3421 cacagcggaa taatggcaat ctgcatatgc aacaaccagc gccacagcgg atgcgtggag
     3481 attcggcgga tggcatcgct acaggccagc aatgccagcg ccgcagccag ccagaaacca
     3541 ctgccgaggc tggtacgcgc cagcgcactg ccattttgcg ccagttgtgt cgccgccttt
     3601 cccgcgcccc acaccagcaa tacgaagacg aattgcgcca gaatgagtgc acaaatgctg
     3661 ccttttttac cgggaataaa acaggccgtc agccaggcgc aaccaacgcc caccagcatc
     3721 cagatcgttt gcggccacag ctgccagaga tgacgcccct cacccgaaac taaacgatta
     3781 ggcgcgtaac tgataaacgg cagcgccgct gcgattgccg tcagcaacag cagcagcgcc
     3841 agaacaggat taatacggaa ataagtcacg ggaaattact tcgtccaccc tttttgtttc
     3901 aggtagtcgg cagccacttt tttggcatcc agtccttcca cagcaatgct ggcattcagt
     3961 tgctgcaatg tttttgcatc gaggctggcg aagactggct gtagccactg tgccatttgc
     4021 ggatactcct tcaacaccga ctcacgcacc actggtgcag gcgcgtagat aggttgcaca
     4081 ccttgcggat cgcttaaggt ttgcagcccc agcgccgcta ccgggccgtc agtgccgtaa
     4141 gccattgcag cattaacgcc agagatttgc tgggcagcgg ctttgatcgt caccgccgtg
     4201 tcgccaccag ccagtgacag caactgatcc tgaccgagct taaagccata ggctttttca
     4261 aacgcgggta acgcatcggc gcgttcgata aactctgccg aggctgccag tttgaaggtg
     4321 ccgccctctt tcagataacg actcaggtcg gcaagcgaag tgagtttatt tttctctgcc
     4381 acatcctgac gcacggcgat agtccaggtg ttatttgcag gcgcgggcgt cagccagatt
     4441 aacttgttgt gctccgaatc gagctttttg actttctcgt aaccttgctg cgcgtttttc
     4501 cacgctgcat cgttttcatc tttaaagaag aaagcgccat tgccggtata ttccggatag
     4561 atatccagtt caccggaagt aatcgccccg cgcaccacag gagtcgttcc gagttgcact
     4621 ttattcaccg tcggtacgcc gtggctttcc agcacctgca agatgatgtt gccgagtagc
     4681 gcgccttcgg tatcgatttt tgaaccgact ttaacggggg aagccgcctg tagcggcagg
     4741 ctcacggctg ccaacataac cagtgaacct gcccagacct ttgagagtgg catgatgctt
     4801 tcctcattct tttactgttg ttttcagcga attaagagaa aagcatagtt gataatgaca
     4861 gagttagcct gagcggaagg attcagttgc agaatcagat aaatacttaa agggcatatt
     4921 ttgcccggcg tttcttgccg gatgcggcgt aaacgcctta tccggcctac aaaggtcgaa
     4981 aattcagcaa gttaagatat gaacatcagg caaagggcgc aaacgtcgcg cccttactaa
     5041 agcattacag caactcaaac tcgcctttct taacgcgtgc ggaatcagtg ccgataaaga
     5101 cattgaactt gccaggctcg gcgtcatatt tcatctgttg attccagaac ttcagcgcct
     5161 cgatatcaat cgggaagctg acagtctgag tttcgcccgg tttcagggtg attttctcaa
     5221 agcctttcag ctgcttcact ggacggctca tggaagccgt cacatcctgc aagtacatct
     5281 gtactaccgt ggccccttcg cgtttaccgg tgttcgtcac ctgcacgctg gccgtcactt
     5341 tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc agagacggtg aaagtggtat
     5401 agctcagacc atagccgaac ggataaagcg ccccgttagc ttcatcaaaa taacgcgaag
     5461 tgtatttgtt cggcttgtcg gcattatacg gacgaccggt gttcagatgg ctgtagtaca
     5521 ccgggatctg cccgacagaa cgcgggaagg acatcggcag cttgccggac gggttgtaat
     5581 cgccaaacaa cacatcggca attgcattac cgccttcagt ccccgcaaac caggtttcca
     5641 gaatcgcatc agcctgctga tcttctttca ccagcgccag cggacgcccg ttcatcagca
     5701 ccagcaccag cggtttaccg gtggctttca gcgccgcaat caagtcacgt tggctttgcg
     5761 gaatagtgat atcggtacgg ctggaggcct cgtgcgccat cccctgtgct tcaccgacca
     5821 cagccaccac catatcagat tgtttcgccg tctgcaccgc ttcatcaatc atctcttgcg
     5881 gcgagcgcgg atcgactttg accgcttctt catactgatt caggaaatcg ataatgcctt
     5941 tgtcactggt aacgttcgcc cctttggcat acagcacttt accgttttca ccgacggcat
     6001 ttttaatccc ggtcagtacg gtcacggatt gatcggcaac accggctgcg gaccagctgc
     6061 ccatcacgtc gcgtttactg tccgccagtg gcccaaccac cgcaatggtg gccgattttt
     6121 tcagcggtaa cgtttcgaga cggtttttca gcaacaccag gctttcgcgc gccacttcac
     6181 gtgcttcttt acggtgcagg cggctttcgg cattggtatc caccgggtca gactctttcg
     6241 accccaaatg gctgtatggg tcgttaaaca atcccatatc atatttaacg ttcagtacat
     6301 ggcgggcagc atcgtccagc tcttccatcg tcactttgcc ggatttgatc aacccaggca
     6361 gatacttcga gtaatactcg tcgctcatac tcatgttgat tccggatttc agcgccacgc
     6421 gcaccgcatc ttccgggtct gccgccgtgc catgtttaat cagctctttg attgcaccgt
     6481 gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg cagaacatct ttcagcagcc
     6541 aggaatcgga ggtggctggc gtgccgttca gcgagttcag cgccaccatc accgcgccgc
     6601 tgcctgcgtc cagccccgct ttgtacggcg gcatataatc attaaacagg cgctgcggac
     6661 tcatatcgac ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt
     6721 tgacgctggt catcaccgag tagcgatctg ccgggctttt accctgcatc gcttccacca
     6781 tggttttacc cattgttgag gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc
     6841 cccagcgcgg atcgcgcgag acatcgacca tcggtgccca ggtcatattc aggccatcat
     6901 ctgccgcttc ataagcagag acacgtccga ccgttttcac tgcatcgagg ttaaaagacg
     6961 aggccagacc gaggctaatc gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa
     7021 agaaaagagg aattttcagg cggcttaatt ccatcacctg atcctgcatg gcgcggatat
     7081 cctgacgggt tacggtgttg aaaatcgccc caacctgacc gtctttgatc atctcgcgga
     7141 tcgcctcttt cgggttatcc gggccgacgc tgattaagcg cagctgacca attttctcat
     7201 caactgtcat tttcttaagc aattcggtga cgaacgcatc ccgcgcttcg ggcgtgaatg
     7261 gatggttgcc gaataaatca tccgccagtg ctggctgcag ggccagactc accgcgattc
     7321 ctactgaaca tagccatttc atatggattt ttcttcctct gttgcccgac gaggcagcga
     7381 aaatggtgca aaaaccgtag tttgccataa gcatgatgga gagagaaaaa gaatgctcag
     7441 tttattgtct gaattttcaa aatattcact cgctgaattg tcatacaagg cgctattcta
     7501 gtttgtgata ttttttcgcc accacaagga gtggaaaatg tcttccatga caacaactga
     7561 taataaagcc tttttgaatg aacttgcccg tctggtcggt cattcacacc tgctcaccga
     7621 tcccgcaaaa acggcccgct atcgcaaggg cttccgttct ggtcagggcg acgcgctggc
     7681 tgtcgttttc cctagctcac tactggaatt gtggcgggtg ctgaaagcct gtgtcaccgc
     7741 cgacaaaatt attctgatgc aggctgccaa tacaggcctg accgaaggat cgacgccaaa
     7801 cggtaacgac tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg acaagctgca
     7861 cgttcttggc aagggcgaac aggtgctggc ctatccgggc accacgctct attcgctgga
     7921 aaaagccctc aaaccgctgg gacgcgaacc gcactcagtg attggatcat cgtgtatagg
     7981 cgcatcggtc atcggcggta tttgtaacaa ctccggcggc tcactggtgc aacgtggccc
     8041 ggcgtatacc gaaatgtcgt tattcgcgcg tataaatgaa gacggcaaac tgacgctggt
     8101 gaaccatctg gggattgatc tgggcgaaac gccggagcag atccttagca agctggatga
     8161 tgatcgcatc aaagatgacg atgtgcgtca cgatggtcgt cacgcccacg attatgacta
     8221 tgtccaccgc gttcgtgata ttgaagccga cacgcccgca cgttataacg ccgatcctga
     8281 tcggttattt gaatcttctg gttgcgccgg gaagctggcg gtctttgcag tacgtctaga
     8341 taccttcgaa gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc agccggaagt
     8401 gctgaccgaa atccgccgtc atattctggc taacttcgaa aatctgccgg ttgccgggga
     8461 atatatgcac cgggatatct acgatattgc ggaaaaatac ggcaaagaca ccttcctgat
     8521 gattgataag ttaggcaccg acaagatgcc gttcttcttt aatctcaagg gacgcaccga
     8581 tgcgatgctg gagaaagtga aattcttccg tccgcatttt actgaccgtg cgatgcaaaa
     8641 attcggtcac ctgttcccca gccatttacc gccgcgcatg aaaaactggc gcgataaata
     8701 cgagcatcat ctgctgttaa aaatggcggg cgatggcgtc ggtgaagcca aatcgtggct
     8761 ggtagattat ttcaaacagg ccgaaggcga tttctttgtc tgtacgccgg aggaaggcag
     8821 caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca attcgttatc aggcggtgca
     8881 ttccgatgaa gtcgaagaca ttctggcgtt ggatatcgct ctgcagcgta acgacaccga
     8941 gtggtatgag catttaccgc cggagatcga cagccagctg gtgcacaagc tctattacgg
     9001 ccattttatg tgctatgtct tccatcagga ttacatagtg aaaaaaggcg tggatgtgca
     9061 tgcgttgaaa gaacagatgc tggaactgct acagcagcgt ggcgcgcaat accctgccga
     9121 gcataacgtc ggtcatttgt ataaagcacc ggagacgttg cagaagtttt atcgcgagaa
     9181 cgatccgacc aacagtatga atccggggat cggtaaaacc agtaagcgga aaaactggca
     9241 ggaagtggag taaaaattac ggatggcata gtatcgccat ccgtcattca cttaatcgtt
     9301 ctgtgccgtc tgccccgccg ccgccatttg ggcggctttt tgttttttat agctcaacgc
     9361 tgctgctgga acaggcatca ctttaccggt ttcaatccag gtacgcaggc ggctggcatc
     9421 ggcaaaatgg gtatatttgc caaacgcatc catcactacc agcgccaccg gtttattatt
     9481 gataaccgta cgcatcacca gacaatggcc cgccgcattg gtaaaaccgg ttttggttaa
     9541 ctgaatattc cagttatcgc gatacaccag atggttagta ttgcggaacg gcagcgtata
     9601 cgtcgggtta gagaaggttg ccatatcttc ccgagtagta cttaactgcc cgatcaacgg
     9661 atattgtttg ctggcaatga gcagtttggt taagtcacgg gcagttgaaa cgttatgcac
     9721 cgacaatccg gtaggttcaa caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc
     9781 attcattgcc ttaataaagg ctttgtaacc accgggataa tggtgtgcaa ggcttgccgc
     9841 cgcgcggttt tctgaagaca tcagcgccag caacagcata tctttacggc tgatttcgct
     9901 attcagtcgt acgcgcgaat agaccccttt catctccggc gtctggctga tatccacttt
     9961 tagtttttca tccagcggca gtcgtgcatc cagcacaacc atcgcggtca ttaatttgct
    10021 gatagacgca atcggacgca ccagatccgg gtggttcgag tagatcactt tgttggtatt
    10081 cagatcaaca atcatcgcgc taccggaggc aatttcaggt tgtgaagcgg tgatagcggc
    10141 tgccgttttc gcaacggcct gcggtgccaa aggcacagcc agcatcaggg ccaggctaaa
    10201 taaagaaact cgaaatttcg gcatgatgag cattcagata gtggttcacg cgcacaggtt
    10261 gcgcaccgcc ggagtaagga tttactgagg ctagcgacgc catcataacg agcaaaaagt
    10321 gcgatcgtca aaggagaatc gtgaggaaat gctgcattgc tgacatttac gccagcaatg
    10381 caacgtcaaa gaactttcta gaacaaacga taaccgtagc cccatagtat aacggttagg
    10441 gcgagcagca cttccagtac cagcacgcca atcgccagcg tcgaactaga gaagctaagg
    10501 ccttcctctt tgttgatatt caggaagctc ggaataccaa ggtaaagcag gtagccggtg
    10561 taaaacagcg ccaccgtgcc gaccagcgca cacaaccaga ccagtggata aagcgccacc
    10621 aaaccgctta aaaacagcgg agttgcaacg tagcccgcga agaccatgca gtgcgcaagt
    10681 gacggacgct gcggataatt acgcgccatc caccagatga cccgccccat taccgcaacc
    10741 ccagccagca taacgccata aaacaggaca gccagcgcca gtccggtaaa ccaggataac
    10801 ttcaggatag tgccatcgcc aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa
    10861 atcaccggaa tcgccgccat cagcaaaacg tggtgggtgt aatggtgaga aatcgtttcg
    10921 ttttcgcgat taatcacctg catttcacga tcgggatggg aaaacagtcc ccagacatgg
    10981 ctcataccgc ccccttgttg tgagttcatg aacctgacag ttcaagtata agtcagcttg
    11041 tgattatttt ttgttcgctc atgtaatttg caccgtctga tgtacgtcct ttttcagcgt
    11101 gaatgatttc cagggtgtat gcttacaggc aaccaaaggg agacagactg gcctatggat
    11161 ctcaatacac tcatctcaca atatggttat gccgcgctgg tgatcggtag cctggcggaa
    11221 ggtgaaaccg tgactttgct gggaggcgtt gcggcacatc aggggctatt aaagttcccg
    11281 ctggtggtac tttctgtggc gcttggcggc atgattggcg accaggtgct ttatctgtgc
    11341 gggcggcggt ttggcggcaa gctgttacgc cgtttctcga aacatcagga taaaattgag
    11401 cgggcgcaga aacttatcca acgccatccg tatctgtttg tcattggtac gcgctttatg
    11461 tatggctttc gggtgattgg cccgacgctg attggtgcca gccagctgcc gccgaaaatc
    11521 tttctgccgc tgaatattct cggcgcattt gcctgggcgt tgatttttac cactattggt
    11581 tacgctggtg gtcaggtgat tgcgccgtgg ttgcacaatc tcgaccagca tttgaagcac
    11641 tgggtctggt tgattctggt cgtggttctg gtggtgggcg tgcgctggtg gctgaaacgg
    11701 cgcgggaaga aaaagccgga tcatcaggcg taaaaccatt gccctgggta aggcgttcgc
    11761 gccgcatccg acatccggtg caccatgcct gatgcgacgc ttgtgcgtct tatcatgcct
    11821 accaaaattt tacccattga aataaccacg cacctcactg cgctattctg ggttgaactg
    11881 cggattcgcc aacataaagc cgccatccac tatcaacgac tgcccggtgg tgtaatttgc
    11941 gccctctgag cacagccacg ctaccaggct ggcaatctca tgcgttgcgc caaaacgccg
    12001 cagaggaatc gaaggctccg cgtcgggctt cacgtcgctg tcatccatgc cattcattgg
    12061 cgtggcgatc gccccaggcg cgactgcgtt caccaaaatc ttatgcctga ccagctccag
    12121 cgccatcgct ttggttaacc caccgagcgc atgtttagcg gctgtgtagg cgctggcatc
    12181 cggcagcggc gtatgttcat gtaccgatgt aatgttgatg atgcgaccgc cctgcccttg
    12241 tttcaccatc tggcgggccg caatttgtga gcataaaaat gcaccatcga catcaacggt
    12301 aaaaatcttg cgccactcat cgaaagccat atcaagaaac ggcgctttgg tcattgcgcc
    12361 cgcattattc accagcacat caatacgccc cagccgttga atgagtttct ccagcgcctg
    12421 cgcaccttct ggcagtttac cgagatccag ctgcacgatc tccgcacgta cgccgtggct
    12481 aactacctca cgcgcggtat cttttgcccc ttcttcatct gagtgccagg taatgccaat
    12541 atcaaaccct tgctgcgcca gtaataacgc gcactctttg ccgatccccg aatcggaggc
    12601 ggtaataatc gcaacctgtg ccatcgagtt ctccacttaa cgctgaataa acgttaagta
    12661 tagaaggcgc atatcatcag cgtttgtaac ccccgcccaa cgcaccagtg agttgaatgg
    12721 aggcatccag ccactgccct tgcaataaca ggccattggc ccgctcacgc agcgcgggga
    12781 ttctggcttc gctgacgcgg gaaccagcaa tgatgcccgc gttaaagcgc gcctgcgcaa
    12841 gacctaccac acgcaaggta tcgcgctcaa tttgcgcctg atgctggttt ttctccgcaa
    12901 gtgtctgaac ctgactggct gcccgcgcca cgtcattcac cgcttcaacc accgctttgt
    12961 tgtagctggc gatagacaag ttgctttcgg cttttgcgat atcgagattg gcgttaagac
    13021 gaccactatc gaaaatgggt agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat
    13081 gacggaacag atcgcttaag tgcaacgcat cctgttgcag gaaggccatc aggttgatgt
    13141 caggataaaa tgccgctttt gccgcatcaa tggtgcttag cgatgactca acgtaccagt
    13201 gcgccgcctg caaatctgcc cgccgggcca gtaaggagta ccccagttca tcaggaagct
    13261 ggcttgccac tttcggc
//
LOCUS       DAJTYD010000101        13163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTYD010000101 DAJTYD010000000
VERSION     DAJTYD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.101"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO585_002633"
                     /pseudo
     CDS             complement(1..1862)
                     /gene="speC"
                     /locus_tag="OO585_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2260..2958
                     /locus_tag="OO585_002634"
     CDS             2260..2958
                     /locus_tag="OO585_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6630175.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3064..3139
                     /locus_tag="OO585_002635"
     tRNA            3064..3139
                     /locus_tag="OO585_002635"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3097..3099,aa:Phe,seq:gaa)
     gene            complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO585_002636"
     CDS             complement(3288..3824)
                     /gene="yghD"
                     /locus_tag="OO585_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCR6630176.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO585_002637"
     CDS             complement(3826..5004)
                     /gene="gspL"
                     /locus_tag="OO585_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCR6630177.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO585_002638"
     CDS             complement(5001..5978)
                     /gene="gspK"
                     /locus_tag="OO585_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCR6630178.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO585_002639"
     CDS             complement(5981..6580)
                     /gene="gspJ"
                     /locus_tag="OO585_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCR6630179.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO585_002640"
     CDS             complement(6577..6948)
                     /gene="gspI"
                     /locus_tag="OO585_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCR6630180.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO585_002641"
     CDS             complement(7008..7508)
                     /gene="gspH"
                     /locus_tag="OO585_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCR6630181.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO585_002642"
     CDS             complement(7512..7967)
                     /gene="gspG"
                     /locus_tag="OO585_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCR6630182.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLARTVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO585_002643"
     CDS             complement(7984..9207)
                     /gene="gspF"
                     /locus_tag="OO585_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCR6630183.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQRGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO585_002644"
                     /pseudo
     CDS             complement(9207..10699)
                     /gene="gspE"
                     /locus_tag="OO585_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO585_002645"
                     /pseudo
     CDS             complement(10699..12759)
                     /gene="gspD"
                     /locus_tag="OO585_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12789..>13163)
                     /locus_tag="OO585_002646"
     CDS             complement(12789..>13163)
                     /locus_tag="OO585_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein C"
                     /protein_id="HCR6630184.1"
                     /translation="TNKKAVSDEAKQAVAEPAVSAPVEIPTAVRQALAKDPQKIFNYI
                     QLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIALNQQDFTDPRAMIALMRQLPS
                     MDSIQLTVLRKGARHDISIALR"
BASE COUNT         2946 a         3798 c         3399 g         3020 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcaaagtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaactgcgc caacatctct accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acactgatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc tccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cggcgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatctttc gccaataagc
     1381 agatcgccca attttacgtc agcgttacac atatcggcgc gaaagacgtt ctcaccaaag
     1441 aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa acgcaccatg ttgatgtcca
     1501 gggcaagcaa aggtgctgtt gcccatctca acgtactgcg tcagcgtgtc ataaaacggt
     1561 ggcagcaaat tctcttcata ctgacaggct gcggattcca gctccagcca ctgctgctcg
     1621 ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa cagcatgttc ggaatacaaa
     1681 aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa gaatgccact gcgactatcc
     1741 gcagcggtaa tgacgactgc cacgacgtcc gtaaaatcag tatctcccaa cgccaccacg
     1801 cgacgatgag aagaaagtcg ggataccagt tcactactgg cggcaatatt cattgatttc
     1861 ataagcgcaa acccgtttcg gggaagtaag aataccggac aaggtggaaa accctgcccc
     1921 atgagatatg ggtcaaactg gtcaccagct ccgaccgcca gacatcagta aaagcagaaa
     1981 cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta acttcaccgc atgagcagta
     2041 acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc gagagaatgt aaggaggtgg
     2101 cgtgcatcgg gcaaactccg tacagagagg agaaaattcg cgcaatcatg gcacctttcg
     2161 ctaaagcgtg caagccagga ctttgcgacc agacaagcca tcaacaaggc aaaaagttca
     2221 taataagaaa atcaaacaat acagttaaca ggaactcttg tggtcatcgg cccttttatc
     2281 aacgctagtg cagtcttact gggtggcgtt ctcggcgcac tgctcagcca acgcttaccg
     2341 gaacgtatcc gcatctccat gacatctatt tttggtctgg catcgctggg gattggtatt
     2401 ttactggttg tgaaatgcgc caaccttcca gcgatggttt tagctacgct acttggcgct
     2461 ctaattggcg aaatttgttt gctggagaaa ggtgtcaata cagcagtcgc caaagcacaa
     2521 aatctgtttc gtcactcacg taagaagcca acgcatgaat cttttattca gaattatgtc
     2581 gcgattattg tcctgttttg cgccagcggc accgggatct tcggggcgat gaacgaaggg
     2641 atgaccggcg atccgagtat tttaatcgcc aagtcatttc ttgatttctt tacggcgatg
     2701 atcttcgcct gctcgctggg tattgcggtg tcggtaatta gtatcccatt actgatcatc
     2761 cagttaacgc tggcgtgggc tgccgcgctg atattaccgc tgaccacacc gtcgatgatg
     2821 gcagacttca gcgccgtagg cggtttattg ctgctggcaa ccggattacg catctgtggc
     2881 attaaaatgt tcccggtggt caacatgctt ccagcactcc ttctggcaat gccgctttcc
     2941 gccgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt
     3001 ctgcagtaaa ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc
     3061 gttgcccgga tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg
     3121 attccgagtc cgggcaccac taattcttaa gaacccgccc acaaggcggg tttttgcttt
     3181 tggatctgac aataaccttc acgaaaaaag tttgcttata aagtctgggg gaattactct
     3241 cgccacgtta acgagagtaa ttttattgat actaatctcc tgatacttta cccccgtcca
     3301 aactccagcc gctggacatt caccatccca gtcttctcag cagcactggc atcaatttgt
     3361 gtcacccgca gcgcatactt ttcatccagc gcgtttaacc agttcagcag gtcattaaac
     3421 accaccggat ctatccagac ctgaatattc tccccacgct cggctatccg cttgatgact
     3481 accgagtgcg cggaagcact gtcactgata acccgcgata cctgcgcagg cgttgttgtg
     3541 gcggattttc gcgccgcaat aatatccggc gcggcgctcc tcagtctggt gttcatcgcc
     3601 accagttgct gcaacattgt ctcctgttgt actatccgtt cgctcaacgg ctgccagatg
     3661 aggacgtaat atccgacgct aaacaggaac accaccgctg ccagtaacat gcccttttca
     3721 cgcggagaac gccccgccag gtgttgtgcc agccagtgtt cgccacggct taactggcgt
     3781 tcacgccatt gctgaaaata gtgaataaat ttatcgcgta acatgttatt tcctccgcaa
     3841 cgttacgccg ccggaaaccg catcaccctc tttctgtaac gcgtcctgtt gcacaacata
     3901 atcggttgcc agtgcactac gcagtttgtc gaaactggca aagttcgcgg cccgtagctg
     3961 gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca cgcatttcga tgtcggaaag
     4021 tgacgctgat ttcagggtgc tggctatcgc tgacaactcg gcgagcagcc gggtatcgtc
     4081 ggcctgtggg cgatattttt tcagcgccat cgtcacctga gagcgtaaat tcacaatccg
     4141 cttctgctcc gggaacaacg ttaagaactg ttcctccgcc tgggtgcggc tttgcgccac
     4201 ctgttcgctg acgctccata acgtcacgcc ccgctccact gccagcgcaa ctagaatcag
     4261 caatatcggc agaatcatca cccgccagcg cgcccactgt tttcggtagc tgacgcgagg
     4321 ctgccacggc cctgtcagca ggttcccttc cggttcgcca taagtggtaa cggcgggcag
     4381 agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat gctgatagct tttccggtgc
     4441 aatgccgact acggttagcg aaagcggtaa atcctgctca ttgagctggg cgcggaacat
     4501 gaccggagcc agcgcccgcc cggcgctcca tccccggcat tcatcaatgc ggcagataac
     4561 ccgttgcgca tcgccagcca taaccccaca aggaatggac atccagtctg gcgcgacggt
     4621 agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg ttgcgcatat gctgttggtg
     4681 aatcactgct acggttgcca gttgctggtc gatattcaac ggggcgaaat gcagttcatc
     4741 gatatcctgg ttcagctctt cttccagtaa ggcgggcaga atggtcggta tctgcttacg
     4801 ggcgacatca ggcagttcaa cctgccagac gctgatccat tcgccgggaa tgtagagtcg
     4861 aatcgcatcg gtttgcagcc attgctggag ctgttcgtta gcaacgtcag accagatacc
     4921 gtgctccaca tcgggtgtac ggcgctgcca atggatggga tcagcggcgc aaagcgggaa
     4981 aaaaatctca aggatggaac tcactcactt tctcctgtct gatgccagag aaccgaaaag
     5041 tgttgtgggc ccatgcggac aattaacgag ttcatcgtca gttcaatctc attcacggtg
     5101 atatctgaac gcagccagaa gtaattgctg tccacgctca ggacggtttt tagctgtttt
     5161 ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa gaaactgatc gacatcttcc
     5221 cagcccttcg ccggacgttg ttgtaataac gcccgcgcct gaacggggct taaccacggg
     5281 tcaaacagcg cctcaagaat cacactttgc gtgacgtcta aagtattgat gttgatttgc
     5341 tggcggatca tcggcagcgc acagaccagc ggtttcagtt tttgataaag cccggcgtcc
     5401 attccctgta ccacgcgcat ctcactgata tcagccagcg gttgattggc ggcgtagaac
     5461 ggcaccgaac gggcgagata ctcgctgtct tcacggccca gacgtgtctg cacgctgcgg
     5521 tcctcgtcaa taaactccca caggctttcg gctatcagtt cggcccgata agcaggcaca
     5581 tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa gcggacgcga cgccgttgtc
     5641 ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt cacgcagggt gaccgcaatt
     5701 tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc acggctgtgc cagatgcacg
     5761 cgcttttcat ttttcaggct cagactgagc gcgctcagcg ccaggctttc cgcactggcg
     5821 ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg ttcgcccaag ttgttgctgc
     5881 attcgcccgg aaagcgtgat ggttaccagc atcataaccg ccagcaatac cagcaccacg
     5941 accagtgcca ttccgcgttt tggtggtgag gtgatcatga ttattgcggc ccgcgtaaca
     6001 accagatgcg ttcaatctcg ccccattgcg gcgaatgcag ggtcatgcgt accgccacag
     6061 ggatcgcctg tactgacgac cagctctcct gccagcgcgt gccgtcgtag aactgcaaac
     6121 ggagcgaatc ggccggaatc aacttttgca ttgtcggctt cacgctgcct gcggcatcgg
     6181 tcagcggcca ggctaaccgt tcgagataac cgtcatgaat gcggtaaccg acggtgagca
     6241 gattgctgcg cggcagacgc atcaacgggt taaccacacc gccgcgcaca aaacgcattc
     6301 cttcactctc agacgccagc acgccagcgc cagccagtaa agctggttca cgctggccct
     6361 gatcgcctct taccggacgc ggcatcattt gggtcagatc gtgggtcaga aaactcatcg
     6421 tttgctgcat gaggtttagt ttttgatcgt gatcggcgac ggcgctattc acgcgcgtga
     6481 cgccgttagt cacctgctgc gccatcagtg ccagcgaggc aaaaatggca attgccacca
     6541 acatttccag taacgtgaaa ccagcgcgag tccttctcac tgttggcctc ccacggcgct
     6601 aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct tcgtgacggc tgacttcaat
     6661 atccaccgcc tggagcagcg cgttgcctgt attcaatggg attgttcgcc agtaccagcg
     6721 gcttcctgcc atatcgctct cgccctgatg cctgaccgcc cgctgctcac gggtcatcag
     6781 gtagagcagc gcagtctggt tttcagctac ccagcccgct accgtttttt cctcaagaac
     6841 gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc gccgtggcag ccagcgcaaa
     6901 aatcgccagc acgagcatca cttccagcaa ggtaaatccg cgcttcatgg catcctctca
     6961 tcacattgat tgagggataa cgcgccatcg tgtgccagct ttaccgctca acatgcgttt
     7021 tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg gttcaaaagg cgaaaaacgg
     7081 atctgcggcg tcttcttttt cgcctctttt tgcaactcca gttcaatatc gtgcagcgtc
     7141 aggcgacgac gttgcagctc cagcgcatat tccttctgcc agatatcact tccgggttgt
     7201 aacagcatct gcacctgttt cggcaccgta acctgtgccg ataaccgggt cgcagaaacg
     7261 ggcaaccact gcccctgacg acgctgcata aactgatagc caggcgggtc gattagcaca
     7321 ccgagtgtct gtccttcaat cactgccctg tccttaaact gcgcaaagcg agtcagaaaa
     7381 tcctgtgccg cttttttcgc gggcggttca gaatcggtcg caaacgtctg taccacgccc
     7441 gcactggcaa ggccgataag gaaaatcacc agcatgattt ccagaagtgt gaatccgcgt
     7501 tcaggcactg cttactgaaa ctcctgcaaa ttccagttac cgatatctgc gccagcgcct
     7561 tccccgtttt cctgaccatc cgcccccagg gtataaacat caaacagtcc tttttcaccc
     7621 gggctgagat actgataatc attgccccac ggatcctttg gcagtcgttt gatgtatcca
     7681 ccagtacggt agttgcggga gtccgccata ttggctggct gctggatcag cgcttccagc
     7741 ccctgttctg tggtcggata acgcccgtta tccagtcggt acatatctag tgcgttttcc
     7801 agcgccacga tatcgctgat ggctttttgc cgattggctt tctctttgtt gcccaacagg
     7861 ttaggcacca ccgtccttgc caggacgcca agaataacaa tcaccaccat cacttccagc
     7921 agggtaaaac ctgcccgtgg tttgtgtgtg cgggataacg aattcatgtg ataactccgt
     7981 aaattacatt ccaaccatat tattcagttg cagcatcggc tcgaggatgg cgatgacaat
     8041 aaacagcacc acgcccgcca tcatcaccac cagcgcaggc tcaaacagcc ccaacgccag
     8101 ccccacctgg gtatcaaatt cccgttcctg gttaacagcg gcctgctcaa gcatggtttc
     8161 cagttcgcct ctctgttcgc cggaagcgat aatgtacagc atcatcggcg ggaacaggcg
     8221 caactccgcc agcgcggcgc gcagactgct tccttcgcgg acgcgatctg ccgccagcag
     8281 cagttgttgt tcgacatagc gatttgccga cacggcggca gcggtctgaa tgccttccag
     8341 tagcggaaca ctgctggcgg tgaggatgct taatgtgcgg gaaaaacgcg ccgtattcag
     8401 cccgcgcgca acacggcccg tcacgggcag acgcagcaag gttttatccc agcgcaggcg
     8461 catagtagga tttttaagta gccattgccc cagcaccaga agcgccagca atcccgccag
     8521 ccagtaaacg ccgctggcct gtaacgcatc actcatagcg ataagcgcac gggtggagga
     8581 gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt ttcggcacca ctgccgtcag
     8641 caaaatagtg accacgcccg ttgccaccac cagcataacc agcggataga gcatggcctg
     8701 caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa tctgccaggc gattgagcac
     8761 cacgtcgaga tgcccggatt tttctccggc agcgaccatc gaacaaaaca gggaatcaaa
     8821 gacgcgggga tgttcgcgca ggctgtccga cagggtataa ccttcctgaa tccggctgcg
     8881 cagcgccatt ccgaggctct tcacatgcag tttttcactt tgctcactga ccgcctgcaa
     8941 gcaggtttcc aacggcattg ctgcctgcac cagcgttgcc agttggcgcg tgaacagcgc
     9001 cagatctgcc gccgccacgc gacgatgtgc gtgccgccga cgctgcaaca ttccccctgc
     9061 cgatgcattc atcctggctt caatgtgcac ggggataagc tctttaccgc gcaacaactg
     9121 gcgggcatga cgcgcggaat ccgcctcaat catgcctttg gttttgcgac cattacgctc
     9181 cagcgcctga tagtaaaaca gtgccattac gcctccatgg ttacccgcag aacttcatcg
     9241 agagaggttt ctccggcgag cactttctca atgccgttgc tgcggatacc cgcagagtgt
     9301 tgccggacat aacgttccag ctccagctcc ccggcctgac ggtggatcaa atcacgcaat
     9361 gtggcatcca ccacgtcagc tcatggatgg cagtccgtcc gcggaaacct ttgtgattac
     9421 aggcggggca gccctgcgga tggtacagag tgacggttcg cgcatcggta atgcccagca
     9481 ggcgtttttc ttcgtcagtg gcaggcgcgg cctgacggca gtcgggacac agcgtgcgga
     9541 ccagtcgctg cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca
     9601 tatcctgcaa acgcgtgatc gcccccaccg ccgtgttggt atgcagcgtg gaaaggacca
     9661 ggtgtccggt aagcgatgcc tgaacagcga tttctgcggt ttcggtatcg cggatttcac
     9721 cgaccatcac cacatccggg tcctgacgca aaatcgcgcg caggccacgg gcgaaggtca
     9781 tgccgacgcg ggtgttaacc tgtgtctgac cgatcccttc aatcatgtat tcgatggggt
     9841 cttcaaccgt gagaatgtta cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg
     9901 tggtgctttt gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta
     9961 acagctggcg caactgcacg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc
    10021 gggcctggtt tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg
    10081 tggagacacg cacgtcaatc gcccggccgc ccagcagcag cgcaatacgt ccatcctgcg
    10141 gtacgcgctt ttcggcgata tccagccgtg ccatcacctt gatacgcgat accagcagcg
    10201 aggccagttt gcgccccggg cgcagcattt catgtaatgt gccgtcaacg cgaaaacgga
    10261 tcaccagact cttttcaaac gtctcgatgt ggatatccga agcgccttct ttaatcgcct
    10321 ctgccagcat ggcgttgatc agtttgatga tcggcgcatc gtcgtcactt tccagcagat
    10381 cttccgtttc tggcagttct tccgcgagag taaaaaagtc ctcagcagaa ccgagatctt
    10441 ccatcagctg gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa
    10501 attcggcctc gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct
    10561 gcaaaacaga gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg
    10621 ccaccaggct aaaccgacgg ctgaaactgt agggcagacg cacggtcttg gcggtggttt
    10681 cctgtgctac aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg
    10741 cggtaaggcc tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa
    10801 accttgctca tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac
    10861 gccgtctgcc gccataccgt catgcagaat ggtcgggcgg ataaacacca tcaggttacg
    10921 tttttctttt ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag
    10981 cagaggcact ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat
    11041 cagctcacca tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc
    11101 gaggctggtc tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt
    11161 accttcgtta atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt
    11221 attgaaagga ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtccta
    11281 gcccaccatg aagaaggctt cctggttgtc cagcgtagtg atgctcggcg tggagagaac
    11341 gttcgagctg gagtcgtttt taaccgcctg caccagtgcc atccagtcgc ctttcaccac
    11401 gccaaccgcc gtaccgctaa atccagaaag aagctgggcg agtgtggaga gatcgccgtt
    11461 ggtatccgga ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg
    11521 tttcgcctga gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa
    11581 ctgcattaat ccggcatctt tcgatgccca ctgcacgccg aagttgatat tgctaccttc
    11641 ggcaacttcc acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc
    11701 aattacgctt tgcagcgact gcatgatgtc ctgcggcgcg gttacaatca gggcattact
    11761 gtgtttgctg gcggcgatgg atacaacctc acgcccgcta ccgaccgtgc cttccgcctc
    11821 ttctttagcc gccgtgagcg tgccgctgac ctgtttcagc acatcgacca gatcttcggc
    11881 tttgctgtat ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag
    11941 ccgacggatc aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact
    12001 gttggtgcgt tcgtcggcga caatttgaga tttcagcgtc gcaggctggt tctcgccgct
    12061 gtttttcgtc aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg
    12121 gatcacctct tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ccgtcaggcg
    12181 ctccacaacg gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac
    12241 gttgcctgag cctgcgctgt cgatcatctg gcgcagaatc ggtgccagct cgcgtaccga
    12301 cacattacgt accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc
    12361 ttcaccgacc agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac
    12421 gtcgttttcc atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg
    12481 gtaatactgg cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc
    12541 cggccccata atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag
    12601 gtcggtatct ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca
    12661 taatgccgct gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt
    12721 cttacgccag accgacaagg taatatcacg ccaaaacacg atgaatgatt ctcctggatt
    12781 aaatgcggtt agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta
    12841 agttgaatgg aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca
    12901 gtgaaatcct gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg
    12961 aacagagaac gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca
    13021 ggcgtaagct ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg
    13081 gcagtcggta tctcaactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg
    13141 tcactgacag cttttttgtt ggt
//
LOCUS       DAJTYD010000102        13156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTYD010000102 DAJTYD010000000
VERSION     DAJTYD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.102"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..161)
                     /locus_tag="OO585_002647"
     CDS             complement(<1..161)
                     /locus_tag="OO585_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6630185.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQG"
     gene            complement(549..1295)
                     /gene="cutC"
                     /locus_tag="OO585_002648"
     CDS             complement(549..1295)
                     /gene="cutC"
                     /locus_tag="OO585_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6630186.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1309..1875)
                     /locus_tag="OO585_002649"
     CDS             complement(1309..1875)
                     /locus_tag="OO585_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6630187.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2091..3824
                     /gene="argS"
                     /locus_tag="OO585_002650"
     CDS             2091..3824
                     /gene="argS"
                     /locus_tag="OO585_002650"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6630188.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4001..4489
                     /locus_tag="OO585_002651"
     CDS             4001..4489
                     /locus_tag="OO585_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6630189.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4609..5001)
                     /gene="flhe"
                     /locus_tag="OO585_002652"
     CDS             complement(4609..5001)
                     /gene="flhe"
                     /locus_tag="OO585_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR6630190.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5001..7079)
                     /gene="flhA"
                     /locus_tag="OO585_002653"
     CDS             complement(5001..7079)
                     /gene="flhA"
                     /locus_tag="OO585_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR6630191.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7072..8175)
                     /gene="flhB"
                     /locus_tag="OO585_002654"
                     /pseudo
     CDS             complement(7072..8175)
                     /gene="flhB"
                     /locus_tag="OO585_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8258..8902)
                     /gene="cheZ"
                     /locus_tag="OO585_002655"
     CDS             complement(8258..8902)
                     /gene="cheZ"
                     /locus_tag="OO585_002655"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6630192.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLAEARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8913..9302)
                     /gene="cheY"
                     /locus_tag="OO585_002656"
     CDS             complement(8913..9302)
                     /gene="cheY"
                     /locus_tag="OO585_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6630193.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9317..10255)
                     /locus_tag="OO585_002657"
     CDS             complement(9317..10255)
                     /locus_tag="OO585_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCR6630194.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10258..11070)
                     /gene="cheR"
                     /locus_tag="OO585_002658"
     CDS             complement(10258..11070)
                     /gene="cheR"
                     /locus_tag="OO585_002658"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6630195.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11208..11774)
                     /gene="flhC"
                     /locus_tag="OO585_002659"
     CDS             complement(11208..11774)
                     /gene="flhC"
                     /locus_tag="OO585_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6630196.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11777..12127)
                     /gene="flhD"
                     /locus_tag="OO585_002660"
     CDS             complement(11777..12127)
                     /gene="flhD"
                     /locus_tag="OO585_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6630197.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            12905..>13156
                     /locus_tag="OO585_002661"
     CDS             12905..>13156
                     /locus_tag="OO585_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCR6630198.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTF"
BASE COUNT         3395 a         3501 c         3086 g         3174 t
ORIGIN      
        1 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
       61 gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct
      121 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt
      181 ccagaacacc gaaatcattg gataattaaa tacacaacga attaaccgct tgcataactc
      241 ggcacaacac tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa
      301 ttgctaagga ataaccctgt tgttatttag aatatatatt tgtcagtttt agcaatttaa
      361 accccataat taatgtgaat atataaaata tttttagcac aacgctatgt tcactgtcac
      421 ccgctcaaca gatcacggtt atcgtacgtt ttttatactg ctcagggata aaaaaaggcc
      481 cctgtcgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
      541 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
      601 ccgtctacga cataacgcga atactcgtcc gcgtgtgcat ctgatgacat ggacaatcct
      661 tgattacgat aacgcatcgg tgaagcttgc cacgctcccg cggagctatg gacttccagc
      721 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc ggccataatg
      781 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcatct
      841 gatttttgcc ctgatgtcag cacccgggcg atgcccaatt ccgcaagatt actgagtgta
      901 tttaaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
      961 gcagccatta ttttttccat tcgtggcata tcgacattcc cgtcaacatc gagcacgccc
     1021 gtcaccagtc caggaaaacc taattcgcgg actgtgcgca catcctcaag aatggcggca
     1081 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc ggataatcgg atgcacaggg
     1141 atcgtcaccc actggcgcac ggatttcagt acacccaacg acggcgttaa gcccccctct
     1201 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
     1261 tccatgctgt aacagcaaat ttccagtaat gccattttta ctccttaatt acgccgactg
     1321 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     1381 agtgaccgcc agcgtcggat tcggtaatcg ctcatactca ccttttggcg aactggtttt
     1441 cactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     1501 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     1561 acgtttttcc ccgggccacg gtagctccac aatggaaaac tgccagtgcg caacctgtac
     1621 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatatttt ctgacaaaag
     1681 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     1741 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggagagttc
     1801 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     1861 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     1921 cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     1981 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     2041 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     2101 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     2161 gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     2221 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     2281 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     2341 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     2401 gtctcggtgt ttctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
     2461 tggcgaaaga gatgcatgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
     2521 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
     2581 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
     2641 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
     2701 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
     2761 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag atcacctacg
     2821 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
     2881 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
     2941 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
     3001 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
     3061 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
     3121 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
     3181 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
     3241 cgggtggtac agtgaaactg gccgatctgc tggatgaagc cttggaacgt gcacgccgtc
     3301 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
     3361 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
     3421 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
     3481 acacgcgtgt attgtccgtg ttccgtaagg cagaaattaa cgaagagcaa ctggctgcag
     3541 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
     3601 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtgatgtgt gcttacctgt
     3661 acgatctggc tggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
     3721 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctaaagc
     3781 tgggtctgga tacgctgggt attgagacag tagagcgtat gtaatcgatt tttcgtgaga
     3841 gtaaagcctg atcagggtta cctgatcagg cttttttatt gccatctaaa tgtattctga
     3901 aaatggacat gccattgttt tctgactgtt ggataagagg ccagaagcgt aatatccggc
     3961 cccagggaaa cgataacggt tgaatttaag gaataccgca gtgtttaaat ttcttgtatt
     4021 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
     4081 tgacatttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
     4141 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatgcgcaat taaataaagc
     4201 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgtaa aaaaacctga
     4261 tatcgaacaa cttaacttgc ttaaaaaatc acagcgagcc tggctggatt ttagggacaa
     4321 agaatgtgaa ttaatccttt caaatgagga cgttcaggat ttaagtgacc cttattctga
     4381 atcagaatgg ctctcatgta tgatcataca gaccaatacg cgaactcgcc agttgcagct
     4441 ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat tcacatcttt
     4501 ttggcatgtt ttgttgcaag ctattcctga taaataattg caacaagaca tcgagccttt
     4561 ttcactgagt tattaaacat actcgcgagc gcgtaatttt tttgtccttc agcgataatt
     4621 cacaatcact tcattacgtt gtacctttag cggtggaatt aaccgcccac cgcctggcac
     4681 ttcccagata aatcgcaacg gttctgctgc cgctataccg gaaaaggcca cggtggttcc
     4741 gctctgcccc tctaattcga cacaacgaga ttgcgaacac aagcgaaccc gcagtcctga
     4801 aggtgtcggg ccgataagct gataacgcca cgctaccagc gtcatcaatc ctgaagcagg
     4861 ttgtcgcgta gaaagaggcg cagacgacat cgactcaccg cgatgattta gcgtaattcc
     4921 tacactactt gcctgccaca tcccctcccc ggcggcttgc accagcagcg gaaacaataa
     4981 tattgctaat aaggttctca ttatttgccg ccaattgtcg ccgtcatgcg gatatgtcgg
     5041 ttatcagaca gttccagatt cgacaggacc actaactgcg gcaagctgcg gcgcaggaag
     5101 cgagacaata atggtcgcag cgcgtggttc accaacaata ctggcggcgc acccagcatc
     5161 tcctgacggg atagcgcttc ctgagtttgc gccagtaaac gatccgccag ccctggctcc
     5221 agtcctcccc cgccctgcag cgcctgtagt aacaaacgtt ccagcggtgt atcgaggcca
     5281 ataacatgga cttcatcttt gccaggaaac cactgctggg taatcgcccg tcccaacgcc
     5341 acgcgcacga cggcggttaa ttcatgtgga tcgctttgga tgggcgcatg ttccgccagc
     5401 gtttcgagaa tggtgcgcat atcgcgaatc ggtacttttt catcgaggag attttgcagc
     5461 actttatgca gtgtggtgag cgtgacgacg ccaggaacga gatcttccgt cagctttggc
     5521 atctcctggg cgacgcgatc caacagctgt tgcgcctcct gacgaccaaa cagctctgcg
     5581 gcatgctggc taatgaggtg gttaagatgc gttgctacca ccgtgctggc ctcaaccact
     5641 gtgtacccct gaatctgcgc ctgttctttt agcgcacttt caatccagat agcattcagg
     5701 ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc cggcagcggt tccagggtta
     5761 atcgccaacc agcgccccgg ataagcatca ccactgccaa tctccacgcc tttcatcaaa
     5821 atgcgatagc gggcaggttg cagatccata ttgtcgcgaa tgtgcaccac tggcggcaga
     5881 aatcccatct cctgggcaaa tttcttgcgg atactgcgta tacggcccaa caactcacca
     5941 tcctgctgga aatcgaccat cgggatcagt cgataaccca cttccattcc cagagaatct
     6001 tccagttgta catcgttcca cgtcgcttcg acaacggtat tattctctgc catttttacc
     6061 ggtttgggtt cggcaggcgc tttttgttcg catccgcgta tccaccaggc cagccccagc
     6121 aatccggcag tgaacagcaa aaataccagg ttcggcattc caggcaccaa gccgagtaaa
     6181 ccgagcacgg cggcgcttaa caacataacg cttgggttac tgaaaagctg attcaccatc
     6241 tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga tgacccccgc ggcggtagaa
     6301 atcaccagcg ccggaatttg tgccaccaga ccgtcgccaa tggtcaatag cgtataactt
     6361 tccgccgcgt gtcccatgct catgccatgt tgcagcacgc cgaccagcaa cccgccgaca
     6421 acgttaatga ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa cttacttgcc
     6481 ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg ttttttcgcc
     6541 tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg cttacccggc
     6601 ataccatcga gaacaaagcg cgcacccact tctgcgatac gcccggcacc tttggtaatg
     6661 accataaagt tgatgatcac gagaatgaca aacaccacga tacctatagc gaaattgcca
     6721 ccaacgagga agtgaccgaa cgcttcgacc accttccctg ccgccgccgc gccggtatgc
     6781 ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa caacgtagta
     6841 aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa catcgccacc
     6901 agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag tatgaatgcg
     6961 ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc ggcaaggatc
     7021 tgccattgtg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag attactcatg
     7081 ggtcggtttc tcgttaataa aatccagggc ttccggcacc ggaagatgag taggttgtac
     7141 agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagatccagg ccagcacttc
     7201 cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccgcat gtcgatacag
     7261 cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc caatttcacg
     7321 aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc tcattttgtt
     7381 ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga catccgcttt
     7441 cggcacatcg gccatcatcc gacgccgtgc ggcagctcgc tgcatctgac ggatccgtcc
     7501 tttaacatgg gggtcgcctt cgctttgttt gaactcatca cgaatatcct gccgcgacat
     7561 tcgcagcttt ttcaggtggc tgaagatttg gaaaaagacg tcaaatccca ccattggaat
     7621 gacaccaagc accaccagca gtgcgcatag ccctaccaaa tccatcgcat tacccatggc
     7681 ggtaatcgga gactcggcca gtaagcgcat catctgcggc cagtgatgcc agagaaaaaa
     7741 ccccgtcacg ctgccaacca agatggtttt caaaattgct ttaagcaact ccgcgccagt
     7801 ctgtgccgag aacatccgtt taatgcccgg tagcgggttg agtttggaaa acttcggctg
     7861 caaggatttt ccgctaaata ccagcccccc cagcatgacc ggagaaatga gcgctaccag
     7921 caccacgccg ctaatcagcg gtagcagcgc cagcatggct tctctgatca gcagaataat
     7981 ctgcccgagg atcagattcg ggtctttgat aatactgtga tcaaaatgca gcccagcgga
     8041 gagcatgccc gacaatcgac gggccagcga cacaccgccg aaccagataa cacacacgcc
     8101 caccagcaaa atcagcagtg aggtcagttc acgggaacgc gggatttagg gcttttgctg
     8161 ccacacatca agcatagtgt gcgtttgtcg gatgcggcgt catcgcctta tcagaccgcc
     8221 tgatatgacg tggtcacgcc acatcaggca atacaaatca aaatccaaga ctatccaaca
     8281 aatcgtccac ctgatcctga ctggctacca caccggcttt gctggtatcg acctgaggtc
     8341 cattaagcaa actctggttt tcacgttttg gacgcgactc ctgttccggg atgttttcca
     8401 acagcaccat cagcaactgg cgttcgatct cctgaatgac atccatcatc cgcttaataa
     8461 cctgcccggt gagatcctga aaatcctgcg ccatcatgat ttccagcagt tgcgcgttag
     8521 taaagctggt atgcgcgggt acatctgcca gaaattgtcg tgtatctgtt accagttcac
     8581 gggcctcggc aaggtcaatc ggatcggcaa accagtcatc ccaacgttgg gttaacgctt
     8641 ttgctgattt ctccatttga tcctgatgcg gttgtgacgc ctcaacactg ttcagcgccc
     8701 gctccgcagc ctgggcggtc atctgcacaa catagtacaa acgatcgcgc gcatcgggga
     8761 tggcttccgc cgcttcggca atggcctgat ccagccccag ttcccgcaaa ctgtcgcgca
     8821 gcatacgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc tcgtcagcag
     8881 gtttgattga tggttgcatc atagtcgcat cctcacatgt ccagtttatc aaagattttg
     8941 ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc actggccccc
     9001 gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat taacactggc
     9061 aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc atccatattg
     9121 ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt attgagagcg
     9181 tcgacgccat cttccgcttc ctcaacatta ttgaatccca gctctttcag caggttacgc
     9241 actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc tttatccgcc
     9301 atttcacact cctgatttaa atacgtatcg cctgtccggc actaattttt gccaacattt
     9361 gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg atggcctcgc
     9421 gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat gcccccgcct
     9481 gacgcatcgc caacattccc accgggccgt cgtgaatttt gatttgataa tttgcgccac
     9541 tacgcgccag ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac
     9601 gttctccgtc ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa
     9661 aagagcgggt gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg
     9721 gcaacggttg cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa
     9781 tcagtttttc agaactcaac aacggacccg ccttcagcgt tgtcggtgct gacaatggct
     9841 tatgtgctgc aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat
     9901 acgccagcat tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca
     9961 gctccagcgc gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa
    10021 cgggcattgg acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca
    10081 tttcaacatc cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcgacca
    10141 gcggatcagg cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca
    10201 tgatctggcg catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta
    10261 atccttactt agcgcataca ccgtctgacc acgcagcgtg aagcggcgct caaggtggct
    10321 aaagttttca gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg
    10381 gcgcaaaatc tcctgctggg tattttgatc gaagtagatc atgacgttac gacagaagat
    10441 cgcatcaaac ggccccggca cggtgtactg tttcgccagt agattcagcg gggcaaaatc
    10501 aacatagttc gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg
    10561 catgaaatac cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgataaat
    10621 accgcttctg gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg
    10681 tccgggcgcg gtgcccaatg tgtcagccag cgtcatcgca atgctgtacg gctcttcgcc
    10741 ggttgaagcc gccgcgctcc atacgcgata ctcgccagaa cggcgacgtg cgtgatccgc
    10801 gagcagaggg aaatgatgtg cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt
    10861 gataaacgcc tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc
    10921 gaaatccgtc agtcccagcg aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg
    10981 tttatgatcg gccaggacga tcccggctcg ttgatagatc aattgactta tccgccgaaa
    11041 actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt
    11101 tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggca aaaaaaagca
    11161 gcggtacgtc gttaccgctg ctggcatgtt gcgccacacc gtatcagtta aacagcctgt
    11221 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
    11281 ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
    11341 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
    11401 ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagtgg tccttcttct
    11461 gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
    11521 cacaaacctg ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt
    11581 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
    11641 ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
    11701 agcatctgca aacgagcgcc cagggtgatc aattcaatat cccgcgcttc ctgaacaatg
    11761 cttttttcac tcatgatcag gcccttttct tgcgcagcgc ttcttcaggc tgattaacat
    11821 cattcagcaa gcgtgttgag agcatgatgc cggtatgaat ttgctggaga tcgttaacgc
    11881 gggaatcttg cgtcaactga gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca
    11941 gttgattggt ttctgccagc ttaaccattt gcggaagagt cagtgccgct aacgttgtcg
    12001 ccatttcttc atttatgccg agacgaaaca tagcggacgc tttgtcctga acaatcaaac
    12061 gctgtgcaag tagtaaatat gacaagttga tgtcataaat gtgtttcagc aactcggagg
    12121 tatgcattat tcccacccag aataaccaac tttattttta tgcggtttca ccgcaccccg
    12181 tgatgtcgcc gggaagcccc ggtaaaaaat aattagcatt agaatagtgc gataagctgc
    12241 aataagcaga accacctttt tggtttaata tgtccttaca aatagaaatg ggtctttaca
    12301 cttatctaag atttttccta aatcgacgca actgtactcg tcactacacg cacatacaac
    12361 ggagggggct gcgattttca ataatgtgtg atgcagatca cacaaaacac tcaattactt
    12421 aacataaatg tataagccat acttttgttt tgggtgtatt tcaatctgtt aaaaagtttt
    12481 tcgctacgct agcaagcaaa aatgaaactg gaataatcga aatgggatgt tgcgcacagt
    12541 taaaataact caccgtaaat aatcatctgc tataaataat cactttcatg caataccaga
    12601 taagctattt ttaaacagac acttaccgca caacaaacta attaaataca aagaaaggca
    12661 acaatcaact taatgttaat gaaatgtttt acatatatta aacacttgtt taatgcggag
    12721 aatcagtaca ctattttctt ataattacat ttgaaattat atgatcagcg ggagtgattg
    12781 attaattgtc tttactaata atcagactaa tatttacctg tttgaccgag ttgggattgc
    12841 gtcgtttctc cattaggagt aaagctttaa tgtcacctga agttcacaga ataaggaaca
    12901 ggttatgagc tatagcaata ttcttgtcgc tgttgcggtt acaccggaaa gtcagcaact
    12961 gctggcaaaa gcggtatcta tcgccaggcc agtaaaggga cacatcagtt taattactct
    13021 cgcttccgac ccggaaatgt acaatcaatt agccgcgccg atgcttgaag atttacgcag
    13081 cgtgatgcaa gaagaaacgc aaagctttct tgataagtta attcaggatg cggggtatcc
    13141 cgttgacaag acgttt
//
LOCUS       DAJTYD010000103        13116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTYD010000103 DAJTYD010000000
VERSION     DAJTYD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.103"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            308..643
                     /locus_tag="OO585_002662"
     CDS             308..643
                     /locus_tag="OO585_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6630199.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            803..1246
                     /gene="yjdN"
                     /locus_tag="OO585_002663"
     CDS             803..1246
                     /gene="yjdN"
                     /locus_tag="OO585_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6630200.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1379..2167
                     /gene="phnC"
                     /locus_tag="OO585_002664"
     CDS             1379..2167
                     /gene="phnC"
                     /locus_tag="OO585_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6630201.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2192..3208
                     /gene="phnD"
                     /locus_tag="OO585_002665"
                     /pseudo
     CDS             2192..3208
                     /gene="phnD"
                     /locus_tag="OO585_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
     gene            3334..4113
                     /gene="phnE"
                     /locus_tag="OO585_002666"
     CDS             3334..4113
                     /gene="phnE"
                     /locus_tag="OO585_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6630202.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4134..4859
                     /gene="phnF"
                     /locus_tag="OO585_002667"
     CDS             4134..4859
                     /gene="phnF"
                     /locus_tag="OO585_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6630203.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4860..5312
                     /gene="phnG"
                     /locus_tag="OO585_002668"
     CDS             4860..5312
                     /gene="phnG"
                     /locus_tag="OO585_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6630204.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5309..5893
                     /gene="phnH"
                     /locus_tag="OO585_002669"
     CDS             5309..5893
                     /gene="phnH"
                     /locus_tag="OO585_002669"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6630205.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5893..6951
                     /gene="phnI"
                     /locus_tag="OO585_002670"
     CDS             5893..6951
                     /gene="phnI"
                     /locus_tag="OO585_002670"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6630206.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            6944..7789
                     /gene="phnJ"
                     /locus_tag="OO585_002671"
     CDS             6944..7789
                     /gene="phnJ"
                     /locus_tag="OO585_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6630207.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7786..8544
                     /gene="phnK"
                     /locus_tag="OO585_002672"
     CDS             7786..8544
                     /gene="phnK"
                     /locus_tag="OO585_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6630208.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8655..9335
                     /gene="phnL"
                     /locus_tag="OO585_002673"
     CDS             8655..9335
                     /gene="phnL"
                     /locus_tag="OO585_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6630209.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9332..10468
                     /gene="phnM"
                     /locus_tag="OO585_002674"
     CDS             9332..10468
                     /gene="phnM"
                     /locus_tag="OO585_002674"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6630210.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10468..11025
                     /gene="phnN"
                     /locus_tag="OO585_002675"
     CDS             10468..11025
                     /gene="phnN"
                     /locus_tag="OO585_002675"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6630211.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11012..11461
                     /gene="phnO"
                     /locus_tag="OO585_002676"
     CDS             11012..11461
                     /gene="phnO"
                     /locus_tag="OO585_002676"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6630212.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVSSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11463..12221
                     /gene="phnP"
                     /locus_tag="OO585_002677"
     CDS             11463..12221
                     /gene="phnP"
                     /locus_tag="OO585_002677"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6630213.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12368..12697)
                     /gene="yjdP"
                     /locus_tag="OO585_002678"
     CDS             complement(12368..12697)
                     /gene="yjdP"
                     /locus_tag="OO585_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6630214.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12766..13083)
                     /locus_tag="OO585_002679"
     CDS             complement(12766..13083)
                     /locus_tag="OO585_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6630215.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2904 a         3932 c         3663 g         2617 t
ORIGIN      
        1 aattttatat ttagtgtgtt gttttttatc agaataaata acgtcttctg atgcgtttaa
       61 aacgtcagaa agataaaaat atcatgtgaa taaaaaaaga acaggtagag cattaacatt
      121 atcttaaata ataaatagag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
      181 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
      241 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
      301 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
      361 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacacagaac ctgcacagga
      421 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
      481 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
      541 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
      601 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
      661 agccggataa ggcgctcgcg ccacatccgg caacgatgcc gactgcctga tgcgacgctg
      721 accgcgcctt atcaggcttc cctctcatct gtataaattt cgaactacac ttaactggct
      781 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      841 ttccgacgcg attgcctatt atcaacgtac gttgggcgcg gaactgctct ataaaatcag
      901 cttcggcgaa atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca
      961 atttcccgat accgccatcg cccacgccaa cgtgcgcatt gccggaagcg acatcatgat
     1021 gagcgatgct ataccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc
     1081 gcaacaggtc gaagaaggaa aacgctggtt tgataacctt gccgctaacg gaaaaattga
     1141 aatggcctgg caggaaactt tctgggcgca tggcttcggc aaagtcaccg ataaatttgg
     1201 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataacccg ccgggaggcc
     1261 cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt
     1321 aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1381 gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aagcgctgca
     1441 tgcggttgat ctgaacattc atcacagtga aatggtggct ctgcttgggc cgtcgggttc
     1501 cggcaaatcc acacttttac gtcacttaag cggtttgatt accggcgata aatccgccgg
     1561 cagccatatc gagctgctgg gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat
     1621 ccgcaaaagc cgcgcccata ccggctacat cttccaacaa ttcaacctgg tgaatcgcct
     1681 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg
     1741 ttttagctgg ttcacccgcg agcagaaaca acgcgcgtta caggcgctga cccgcgttgg
     1801 catggtgcat tttgcccatc aacgcgtttc caccctctcc ggcggacagc agcagcgtgt
     1861 ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc
     1921 ctcgctggac ccggaatccg cccgcatcgt gatggacacc ctgcgcgaca tcaatcagaa
     1981 cgacggcatc accgtagtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     2041 acgcatcgtc gccctgcgcc aggggcacgt tttctacgac ggcagcagcc aacagtttga
     2101 taacgaacgt tttgaccatc tctaccgcag cattaatcgc atcgaagaga acgcgaaagc
     2161 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2221 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga
     2281 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaata
     2341 gacgccattc ttacaggata tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc
     2401 cccagactac gcaggcatta tccagggaat gcgcttcaat aaagtggata tcgcctggta
     2461 cggcaacctg tcggcaatgg aagcggtgga tcgcgccaac ggccaggtct tcgcccagac
     2521 ggtcgcggcg gatggatcgc caggttactg gagcgtgttg atcgtcaaca aagatagtcc
     2581 gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga
     2641 tcctaactcc acctctggct tcctcgtccc cggttactac gtctttgcca aaaacaatat
     2701 ctccgccagc gactttaagc gcaccgtcaa cgctgggcat gaaaccaacg cgctggccgt
     2761 cgccaacaag caggtggatg ttgccaccaa caacaccgaa aacctcgaca agctgaaaac
     2821 ctccgcgcca gagaagctga aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg
     2881 cgatccgatc gtctggcgca agaatctctc cgaaaccacc aaagacaaga tctacgactt
     2941 ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc
     3001 cccgttccgc gcctccagcg acctgcaact ggtgccgatt cgccagctcg cactgtttaa
     3061 agagatgcag ggcgtaaaaa gcaataaagg actgaatgag caggacaagc tggcgaaaac
     3121 caccgagatt caggcgcagc tggatgactt ggaccgcctg aacaacgcgt taagcgcgat
     3181 gagttcggtg agtaaagcgg tgcagtaatg tgctcgatcg gtcccctctc ccttgaggga
     3241 gagggttagg gtgaggggga aatgcgcacg ctgatgccct catcccggcc ctctcccaca
     3301 gggagaggga gaaaacatta aacggagcca aacatgcaaa ccatcaccat cgccccaccc
     3361 aagcgtagct ggttctcgct tctgagctgg gccgttgttc tcgccgtgct ggtcgtctcg
     3421 tggcagggcg cggaaatggc cccgcttacg ctgattaaag acggcggcaa catggcaacc
     3481 ttcgttgccg acttcttccc gcccgatttc agccagtggc aggattacct caccgaaatg
     3541 gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cagtggttct ctccatcccc
     3601 ttcggtctga tgagcgccga aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc
     3661 ctgatggatg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc
     3721 gtcggtctcg gcccgttcgc tggcgtgctg gcgctattta tccacaccac cggcgtgctc
     3781 tccaagctgc tttccgaagc ggtggaagcg atcgaacccg gcccggtaga aggcattcgc
     3841 gccaccggcg ccaacaagct ggaagagatc ctttacggcg tgctgccaca ggtaatgccg
     3901 ctgctgatct cctactccct ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc
     3961 ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg aagcgattcg cggttttcag
     4021 ttccaacaaa cctgcgccct gatggtgctt atcatcgtca cggtcagcct gctggatttc
     4081 ctctctcaac ggttgcgtaa gcactttatc tgataagcga ggcattgata tctatgcact
     4141 tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca aaacttgagc
     4201 aggagcttcg tcaacactat cgctgcggcg actatcttcc cgccgagcag cagctggcgg
     4261 cgcgctttga ggtaaatcgc cacaccctgc gccgcgccat cgaccaactg gtggaaaaag
     4321 gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc
     4381 cgctcaacgc ccaggcgcgt tttagccaga acctgctgga tcagggcagc catcccacca
     4441 gcgaaaaact gctgtcggtg ctgcgcccgg catctggtca cgtcgctgac gcgctgggga
     4501 taaccgaggg ggagaacgtc atccacctgc gcaccctgcg ccgggtcaac ggcatcgcgc
     4561 tctgtttaat cgaccactac tttgcggacc tcaccctttg gccgacgctg caacgcttcg
     4621 acagcggctc gctgcacgat tttctgcgcg agcaaaccgg gattgcgctg cgccgcagcc
     4681 agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg ccagcgtctt gaaatcccga
     4741 atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg
     4801 cggagtactc cgtcagcctg acgcgcgccg acatgattga attcactatg gagcactgaa
     4861 tgcacgcaga tatcgcgacc cgccagcact ggatgtccat gctggcgcac agccaaccgg
     4921 ctgaactggc agcccgcctg aacgcgctga acatcaccgc caactatgaa gtgatccgca
     4981 ccgccgaaac cggcctggta cagattcagc cgcgaatggg cggtaccggc gaacgctttt
     5041 ttgccggcga cgccacgctg acccgcgccg ccgtacgcct gactgacggc cccctcggct
     5101 acagctgggt gctggggcgc gacaaacagc acgccgaacg ctgcgcgctg attgacgcgt
     5161 tgatgcagca atctcgccat tttcaaaact tatcagaaac ccttattgcc ccactggacg
     5221 ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt gaacgccagc cgggtcgact
     5281 tctttacgat ggttcgcgga gacaacgcat gaccctggaa accgctttta tgcttcctgt
     5341 gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc atgagcgagc cgggcgtgat
     5401 tgtcgccctg catcagctca aacgcggctg gcagccgctg aatatcgcca ccaccagcgt
     5461 gctgctgacg ctggctgata acgacacgcc ggtgtggctt tccgcgccat taagtaacga
     5521 tatcgtcagc cagagcctgc gttttcatac caacgcgccg ctggtcaatc agtcggaact
     5581 ggcgaccttc gcggtaacgg atgaggcgat ttccagcgaa cagctcaacg ctctttccag
     5641 cggcaccgct gttgcgccgg aagcgggcgc gacgctgatt ttacaggtcg tcagcctgag
     5701 cggcgggcgc atgctgcgcc ttactggcgc gggtattgcc gaagaacgaa tgatcgctcc
     5761 gcagctgccg gagtgcattc tgcacgaact caccgagcgc ccgcacccgt tcccgctcgg
     5821 catcgacctg atcctgacct gcggcgagcg gctgctggct atcccgcgaa ccactcatgt
     5881 ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaga aggagatcga cgccgcccac
     5941 gccctgcaag agagccgacg ccggggcaat accgatttgc ctgaactgag cgtcgcccag
     6001 attgaacagc agcttaatct tgcagtagat cgcgtgatga ccgaaggcgg cattgccgac
     6061 cgcgaactgg cggcgctggc gctgaaacag gccagcggcg ataacgtcga agcgattttc
     6121 ctgctacgcg cctaccgcac cacgctggcg aagctggcgg taagcgagcc actcgacacc
     6181 acggagatgc gtctcgaacg gcgtatttca gcggtttata aagatattcc cggcggtcag
     6241 ttgcttggcc caacctacga ctacacccat cgcctgctcg atttcaccct gctggcaaac
     6301 ggcgaagcgc cgacgctgac caccgcagac agcgaacaat ccccgtcgcc gcacgttttc
     6361 agcctgctgg cgcgtcaggg gctggcgaag tttgaagagg atagcggcgc acagccggat
     6421 gacatcaccc gcactccgcc agtttacccc tgttcacgtt cttcccgttt gcagcagttg
     6481 atgcgcggcg acgaaggcta tttgctggcg ctggcctact ctacccagcg cggttacgga
     6541 cgcaatcacc cgttcgcggg cgaaatccgc agtggttaca tcgacgtgtc gattatgccg
     6601 gaagagctgg gatttgcggt gaacgtcggc gaactgctga tgaccgagtg tgaaatggtc
     6661 aacggtttta ttgacccgcc ggatgagccg ccgcacttca cgcgcggcta cgggctggtg
     6721 ttcggcatga gcgagcgcaa agcgatggcg ctggttgacc gtgcgttgca ggctccggag
     6781 tacggcgagc acgcgacagg cccggcgcag gatgaagagt tcgtgctggc acatgccgac
     6841 aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cccactacgt cgatttccag
     6901 gccgaactgg agctactcaa acgtctgcaa caggagcaga accatggcta atctgagcgg
     6961 ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg ccatcttaaa
     7021 agcggtggcg atccccggtt atcaggtgcc gtttggcggg cgcgagatgc cgatgcccta
     7081 cggctgggga accggcggca ttcagctcac cgccagcgtg attggcgaaa gcgacgtgct
     7141 gaaggtgatt gaccagggcg cagacgacac caccaacgcc gtgtcgattc gcaacttctt
     7201 taagcgcgtg accggggtaa acaccactga acgtacggac gatgcgacgc ttatccagac
     7261 gcgccatcgc atccccgaaa cgccgctgac cgaagatcag atcattatct tccaggtgcc
     7321 gatccccgaa ccgctgcgct ttatcgagcc gcgcgaaacg gaaacccgca ccatgcacgc
     7381 gctggaagag tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc gcttcggtca
     7441 tatcgccact acctacgcct atccggtgaa ggtgaacggg cgctacgtga tggacccgtc
     7501 gccgatcccg aaattcgata acccaaaaat ggacatgatg cccgccctgc aactgttcgg
     7561 cgcggggcgc gagaagcgca tctatgcggt gccgccgttt acccgcgtgg aaagtctcga
     7621 tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca tctgcggatc
     7681 gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca tgtttgtctg
     7741 ctccgatacc gattattgcc gccaacagag cgaggcaaaa aaccaatgaa tcaaccgtta
     7801 ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag cgatgtctct
     7861 tttgatttat ggccggggga agtgctgggc attgtcgggg agtccggctc cggaaagacc
     7921 acgctgctga aatcaatctc cgcgcgcctg acgccgcagc agggggagat tcactatgag
     7981 agccgttcgc tgtacgcaat gagcgaggcc gaccgccgtc gcctgctgcg caccgaatgg
     8041 ggcgtagtgc atcagcatcc gctcgacggc ctgcgccgcc aggtgtcggc aggcggcaat
     8101 atcggcgagc ggctgatggc gaccggggca cgtcattacg gcgatattcg tgccaccgcg
     8161 cagaagtggc tggaagaggt ggaaattccc gccaaccgga tcgacgacct gccgaccacc
     8221 ttttccggcg gcatgcagca acgtttgcag atcgcccgca acctggtgac gcatccgaag
     8281 ctggtgttta tggacgagcc gaccggcgga ctggatgtgt cggtacaggc acgcctgctc
     8341 gacctgctgc gcggcctggt ggtggagctg aacctcgcgg tagtgattgt cacccacgat
     8401 ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg gcaagtggtg
     8461 gagagtgggt taaccgaccg cgtgctcgac gacccgcacc atccgtatac acaactgctg
     8521 gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc cttatccggc
     8581 ctacaaatgc gctccccgta ggtcggataa gacgcgtcag cgtcgcatcc gacacccgaa
     8641 ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa aaccttcatc ctgcatcagc
     8701 aaaacggcgt acgcctgccc gtcttacaac gtgcctcgct caccgtcaac gcaggcgaat
     8761 gcgtggtgct ccacggtcac tccggcagcg gcaaatcaac cctgctgcgc tcgctgtacg
     8821 ccaactatct acccgacgaa ggtcaaatcc agatcaaaca cggtgacgag tgggtagacc
     8881 tggtcaccgc gccagcgcgc aaagtggtgg aaatccgcaa aaccacagtc ggctgggtga
     8941 gccagtttct gcgcgtcatc ccgcgtatct cagcactgga agtggtgatg cagccgctgc
     9001 tcgataccgg cgttccgcgt gaagcctgcg ccgctaaagc cgcgcgtctt ctcacccgcc
     9061 tgaacgtgcc ggaacgcctg tggcacctgg caccatcgac cttctccggc ggcgaacagc
     9121 agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc cattctgctg ctcgacgaac
     9181 ctaccgcctc gctggacgcc aaaaacagcg ccgcggtggt ggaactgatt cacgaagcca
     9241 aagcccgtgg cgcagccatc gtcggcatct tccatgacga agctgtacgt aatgacgtcg
     9301 ccgaccgcct gcacccaatg ggagcctctt catgattatc aataacgtta agctggtgct
     9361 ggaaaacgag gtggtgcacg gttcgctgga ggtgcaggat ggcgaaatcc gcgcctttgc
     9421 cgaaagccag agccgcctgc cagaagcgat ggacggcgaa ggtggctggc tactacccgg
     9481 cctgattgag ctgcataccg acaacctcga taaattcttc accccgcgcc cgaaagtcga
     9541 ctggcccgcc cattcggcga tgagcagcca cgacgcgctg atggtggcaa gcggcatcac
     9601 caccgtgctg aacgcggtgg cgattggcga cgtgcgcgac ggcggcgatc agctggagaa
     9661 tctggagaag atgatcaaca ccatcgaaga gacgcagaaa cgcggcgtca accgcgccga
     9721 gcaccgtctg cacctgcgct gcgagctgcc gcatcacacc acactgccgc tgtttgaaaa
     9781 actggttcag cgcgaaccgg tgacgctggt gtcgctaatg gaccactcac cgggccagcg
     9841 ccagttcgct aaccgcgaga agtatcgcga atattatcag ggcaaatact ccctcaccga
     9901 tgcgcagatg cagcagtacg aagaagagca attggcgctc gccgcacgct ggtcgcagcc
     9961 gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgacaa attgcgctcg ccagccacga
    10021 tgacgccacg cacgcccacg ttgccgaatc ccaccaactt ggcagcgtga tcgccgaatt
    10081 taccaccacg ttcgaagcgg cggaagcctc gcgtaagcat ggcatgaacg tgctgatggg
    10141 tgcgccaaat atcgtgcgtg gcggctcgca ctccggcaat gtggcggcca gtgaactggc
    10201 gcagcttggt ctgctggata tcctctcttc cgactactac cccgccagcc tgctggatgc
    10261 ggcgttccgc gtcgccgatg acgagagcaa ccgctttacg ctaccgcagg cggtgaggct
    10321 ggtgaccaaa aatccggcgc aggcgctgaa tcttcaggat cgcggggtga ttggcgaggg
    10381 taaacgcgct gacctggtgc tggcgcatcg ccagggcaat cacattcata tcgaccacgt
    10441 ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt aatggggccg
    10501 tccggctccg ggaaagacag cctgctggcg gaactccgcc tgcgggaaca aacgcagtta
    10561 ctggtggcgc atcgctacat cacgcgcgac gccagcgccg gaagtgaaaa ccatatcgcc
    10621 ctgagtgagc gggagttttt tacccgcgcg gggcaaaacc tgctggcctt aagctggcac
    10681 gctaacggtc tgtattatgg cgtcggcatc gagattgacc tctggctgca cgctggattc
    10741 gacgtagtcg tcaacggttc acgcgcgcat ttgccgcagg cacgggcgcg ctatcaatcg
    10801 gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc tgcgccagcg cctgaaaaac
    10861 cgtggccgtg aaaacaccag tgaaattaac gcccggctgg cgcgcgccgc ccgctatact
    10921 ccacaggatt gccatacgct caacaatgac ggcagcctgc gccagtcggt cgacacactg
    10981 ctgacgctga ttcatcagaa ggagaaacac catgcctgct tgtgagcttc gcccggccac
    11041 gcagtacgat accgacgcgg tttacgcgct gatttgtgag ctaaaacagg cggagtttga
    11101 ccaccacgcg tttcgcgtgg gctttaacgc caacctgcgc gacccgagca tgcgctaccc
    11161 gagcatgcgc taccatctgg cgctgctgga tggcgaagtt gtcggcatga tcggcctgca
    11221 tttgcagttt catctgcatc atgtcaactg gatcggcgaa attcaggaac tggtggtgat
    11281 gccgcaggcg tgcggtctga acgtcagcag taagttactg gcgtgggcag aagaagaagc
    11341 ccgccaggcc ggggccgaaa tgaccgaact ttccaccaac gtgaagcgcc acgacgcgca
    11401 ccgtttttac ctgcgtgaag gctacgagca gagccacttc cgcttcacca aggcgctgta
    11461 acatgagcct gaccctcacg ctcaccggca ccggcggcgc acagggcgtt ccggcatggg
    11521 gctgcgagtg cgtagcctgc gccagagcgc ggcgctcacc gcagtatcgc cgccagccat
    11581 gcagcggcgt agtgaagttt aacgacgcaa tcaccctgat cgacgccggg ctgcacgatc
    11641 tcgccgatcg ctggccgccc ggatcgttcc agcagttttt gctgacgcac taccatatgg
    11701 atcacgtgca ggggctattc ccgctgcgct ggggcgtggg cgatccgatc ccggtttacg
    11761 gcccgccgga tgaacagggc tgcgacgatc tgtttaaaca tccgggcctg cttgatttca
    11821 gccacacggt ggaaccgttt gtggtgtttg atttgcaggg gttacaggtc acgcccctgc
    11881 cgctcaacca ctcaaaactg accttcggtt atctgctgga aacggcacac agccgggtgg
    11941 cgtggctgtc tgacaccgca ggcttgccgg aaaaaacgct gaaattttta cgcaataatc
    12001 agccgcaggt aatggtgatt gattgcagcc acccgccgcg cgaggatgcg ccacgtagtc
    12061 actgtgattt aaacaccgtg ctggcgctga atcaggttat ccgctcgcca cgggtgattc
    12121 tgacccatat cagccaccag tttgatgcgt ggctgatgga aaacgcactg ccgtcggggt
    12181 ttgaggcggg atttgatggg atggagattg gggtggcgtg atgagaggga atgtgcgcgc
    12241 tggccccctc accctaaccc tctccccaga ggggcgaggg gaccgattgt gctcgatatt
    12301 gaatattgcg ctggttttct ccctctcccc attggggtga ggggcgatgc ctgctccata
    12361 cccaacctca tcgcccatac tcatcttcca ttctccgctc ttcatcttct agctgccgac
    12421 gctcctgatc aagctggcgc tggcgatcgt ccagctgcct gcggcgatct tcaaactggc
    12481 ggcggcggtc gtcatattgt ctgcgccgat cgtcgctcac ttcacgctgc cagccgtcgt
    12541 cgcgcgaatc ttcatagtct cgcccacggt cagggttata agcgtcgtta atcgcctgct
    12601 gaatattgcc aatggtgtcg tcgataatat cggcctgggc cggaacggtg gacagcgtga
    12661 gcagggtgaa taaaagaaat agcggaaagc gtttcattag ccaacctcaa aaagaaactc
    12721 tatccacatt aatcattact catccatgca agtagtggat gaatctcaat ttctccgctg
    12781 ctctattgcc gtaatcgcct ccacgcgttg ttgatgacga ccgccttcgt actgtgcgcc
    12841 cagccacgca tccacaatca tttttgccag ttcgaggcca accactcgtg aaccaaaagc
    12901 cagtgcaact tcactggcat aatgcggata atcagtgcgc tctgacgacc tggtgtcttt
    12961 atcaatcacc tcactcattg accatgaata aaaggctggc acagcaacgc agaatcctgc
    13021 ggtgccagcg acatgaccgc ccattgctcc ggtacgtttt ttaaaaagaa acaccggttg
    13081 cattgataaa ttgtgctcag tatttgccga agtaca
//
LOCUS       DAJTYD010000104        12896 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTYD010000104 DAJTYD010000000
VERSION     DAJTYD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.104"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(62..985)
                     /gene="dmlR"
                     /locus_tag="OO585_002680"
     CDS             complement(62..985)
                     /gene="dmlR"
                     /locus_tag="OO585_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6630216.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYLNFSLEHA"
     gene            1088..2173
                     /gene="dmlA"
                     /locus_tag="OO585_002681"
     CDS             1088..2173
                     /gene="dmlA"
                     /locus_tag="OO585_002681"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6630217.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2423..4033
                     /gene="yeaV"
                     /locus_tag="OO585_002682"
     CDS             2423..4033
                     /gene="yeaV"
                     /locus_tag="OO585_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6630218.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4065..5189
                     /gene="yeaW"
                     /locus_tag="OO585_002683"
     CDS             4065..5189
                     /gene="yeaW"
                     /locus_tag="OO585_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6630219.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5245..6210
                     /gene="yeaX"
                     /locus_tag="OO585_002684"
     CDS             5245..6210
                     /gene="yeaX"
                     /locus_tag="OO585_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6630220.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6264..7379)
                     /gene="rnd"
                     /locus_tag="OO585_002685"
     CDS             complement(6264..7379)
                     /gene="rnd"
                     /locus_tag="OO585_002685"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6630221.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7460..9145)
                     /gene="fadD"
                     /locus_tag="OO585_002686"
     CDS             complement(7460..9145)
                     /gene="fadD"
                     /locus_tag="OO585_002686"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6630222.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9350..9931)
                     /gene="yeaY"
                     /locus_tag="OO585_002687"
     CDS             complement(9350..9931)
                     /gene="yeaY"
                     /locus_tag="OO585_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6630223.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(9971..10666)
                     /gene="tsaB"
                     /locus_tag="OO585_002688"
     CDS             complement(9971..10666)
                     /gene="tsaB"
                     /locus_tag="OO585_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6630224.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10724..12634)
                     /locus_tag="OO585_002689"
     CDS             complement(10724..12634)
                     /locus_tag="OO585_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6630225.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12766..>12896
                     /locus_tag="OO585_002690"
     CDS             12766..>12896
                     /locus_tag="OO585_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6630226.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     "
BASE COUNT         3060 a         3465 c         3225 g         3146 t
ORIGIN      
        1 cggcttagaa aggagaaaat caggttttaa cccgatatca acccgataat tgaatcatta
       61 atcaggcatg ctccagtgaa aaattcaggt agtgctcggc aaaatactgg cgtaaaaatt
      121 ctaccgttat ccgcactttc gctgacgtcg ccagccttga aacataaacg gcccagacgt
      181 tcgctggctg gtaatattcc ggtagcactt gcactaaatg accactggca atgttttcgc
      241 taacatccca ccaggaacgc agagcaatcc cctgcccatc gagacaccac tgatggacaa
      301 tttctccatg attagacgac agtgggccgg tgactttaat cgcatggtgg ccttctttat
      361 tgcgtaattg ccagacacca aaaggatgat cgcgctcttt aatgaccagg cacggcaatg
      421 cagaaagatc ggttaaatgt ttcggcgcgc catgttgcgc aataaattcc ggcgacgcgc
      481 agagaatgcg gtaattggtc gccagtttgc gggcgattaa attaggggcg atgtcatcgc
      541 caatgcgaat atcgagatcg acaccttcat tgaccaaatc gaccagccgg tcttccacat
      601 caaaacgtaa ttccagttgc ggatacgcct tcgccagcgc tgataacgcc ggagccacca
      661 cctgtcgtcc aaaaccaaag ctgctgataa tacgcagcat cccctgtggc acctgacgca
      721 cgtcagaaag ttcgtccatc atctgaccga catcctgcaa aatccgctgc gcccattcat
      781 aaatccgctc tccttcttcg gtaatggtga cgcggcgggt ggtgcggtgt aacaacacca
      841 cgtttagcgt ttgctccagc aaggcgatgc gcttgctgac gaacgccggt gaaacgccca
      901 gttcttccgc cacggcggca aaaccagccc gacgagccac cagcataaag acgcgcaaat
      961 cattcagcag cggtaaatta ttcatgattc gtgttttatg tttcaccagt tacggggatt
     1021 aattcctttt ccgtcaatta taggatggtg atgttgtcaa ttttgatggt caggaagtga
     1081 gaacccaatg atgaaaacga tgcgtattgc tgcgatcccg ggagacggga ttggtaaaga
     1141 agtccttcct gaagggattc gcgtgttaca ggctgccgct gagcgctggg gcttcgactt
     1201 gagttttgag caaatggggt gggcgagctg cgagtattac agccatcacg gtaaaatgat
     1261 gccggacgac tggcatgagc aacttagccg tttcgacgcc atctattttg gtgccgtcgg
     1321 ctggccggat accgttccgg accatatttc gttgtggggt tcgctgctga aatttcgtcg
     1381 tgaattcgac cagtacatca acctgcgccc ggttcgtctc tttcctggcg ttccctgccc
     1441 gctggcggga aaacagcctg gcgacatcga tttttacgtg gtcagggaaa acaccgaagg
     1501 cgaatattcc tcgctcggcg gtagagtgaa tgaaggtaca gagcatgaag tcgtcattca
     1561 ggaatcggta tttacacgtc gtggtgtcga tcgcattttg cgttatgcct tcgaacttgc
     1621 gcaaagccgc ccacgtaaga cgctaactta tgccactaaa tcgaacggtt tagccatcag
     1681 catgccgtac tgggatgagc gagtggaagc aatggccgag aattacccgg agatccgctg
     1741 ggacaagcag catattgata ttctctgcgc gcgttttgtg atgcagccgg aacgcttcga
     1801 tgtggtggtg gcgtccaatt tgtttggcga tatcctttcc gatcttggcc cggcctgcac
     1861 cggcaccatt ggcattgccc catccgccaa cctgaatccg gaacgcactt tcccgtcgct
     1921 cttcgagcct gtccacggtt ccgcgccgga tatctacggg aaaaatattg ctaaccctat
     1981 cgccacgatt tgggccgggg caatgatgct cgattttctc ggcaatggcg atgagcgttt
     2041 ccagcaagcg cataacggta ttctggcagc aattgaagaa gtgattgctc acgggccgaa
     2101 aacacctgat atgaaaggca atgccaccac gccacaggtt gccgacgcga tttgcaaaat
     2161 tattttgcgt taaggtcaaa ccagtttatt tgaaccgcgt cactgacgcg gttttttatt
     2221 cgttctttgc agtaaataac ctgcgtcatt tcacctttta ttgtttccgt ttcgtgtttt
     2281 atggctttcc gcattcttaa ttgtttaatt tatgtaatat tcaaataatc aatttgttaa
     2341 acacaacata caactcatta ccctgcaaat aattacaact ggcaaaagcc agagacgggc
     2401 ttttatgccc aggagatgac tcatgatgag caatgttaag aaaaaagatg tgccgctgat
     2461 aagcatcagc ctggtggcca ttcttttcat cgcagctgca ttaagccttt tcccacaaca
     2521 atcggccgac gcggccaacg caatatacac ttttgttacg cgtacgttag gttccgccgt
     2581 acaggtattg gttttgctgg caatgggact ggtgatttat ttagccacca gtaaatacgg
     2641 caatattcgt cttggcgaag gaaaaccgga atacagcacg ctctcctggc tgtttatgtt
     2701 tatttgtgcc ggtttaggtt cttctacgct ttattggggg gttgctgaat gggcctatta
     2761 ttatcagaca cctggattaa atatcgcacc gcgttcacaa caggcactcg aatttagcgt
     2821 tccctactct ttcttccact ggggcatcag cgcctgggca acttatacgc tggcctcatt
     2881 aatcatggct tatcactttc atgtgcggaa aaacaaaggt ctgagccttt ccggcattat
     2941 tgctgctatt accggcgttc gcccgcaagg cccatgggga aaactggttg atttgatgtt
     3001 cctgatcgcc actgtcggcg cactgactat ttcccttgtt gtcaccgcag caacctttac
     3061 tcgtggactt tccgcactga caggtttacc cgacaacttc accgtgcagg catttgtgat
     3121 cctgctttct ggcggcattt tttgcctaag ctcgtggatt ggtatcaaca acggtttgca
     3181 acgtctgagc aaaatggttg gctggggcgc gttcctgctg ccattactgg tgctgattgt
     3241 cggcccaacc gaatttatta ccaacagcat catcaatgcc atcggcctga ccacgcaaaa
     3301 cttcctgcaa atgagcttat tcaccgatcc gcttggcgat ggttcattta cccgcaactg
     3361 gaccgttttc tactggctgt ggtggatctc atacacccct ggcgtagcaa tgtttgtcac
     3421 ccgcgtttcc cgcggtcgta agattaaaga agttatctgg ggactgatcc tcggcagcac
     3481 cgtcggttgc tggttcttct tcggcgtaat ggaaagctat gccattcatc agtttatcaa
     3541 tggcgtaatc aacgtcccac aggtgctgga aacactgggc ggcgaaacag ctgtacagca
     3601 agttctgatg tcgttgccag ccggtaaatt gttcctcgcc gcatacctgg gcgtgatgat
     3661 tattttcctt gcctcgcata tggatgcagt ggcctacacc atggcggcga ccagtacgcg
     3721 taatctccag gaaggtgacg atcctgaccg tgggctgcgt cttttctggt gcgtggtgat
     3781 cactctgatc ccgctttcca tcttgtttac cggtgcttcg ctggaaacga tgaaaaccac
     3841 cgtcgtgctc acagcccttc ccttcctcgt cattttactg gtgaaagtcg gcggatttat
     3901 tcgctggctg aaacaggatt acgccgacat tccggctcat caagttgaac attatctccc
     3961 gcagacaccg gttgaagccc tggaaaaaac accagtgatc cctgcgggaa ccgtattcaa
     4021 aggcgacaac tgagcgccat catcctaacg ataaaggtat ccctatgagc aatctgagcc
     4081 ctgactttgt actacccgaa aatttttgcg ctaacccgca agaggcgtgg accattcctg
     4141 cccgttttta taccgatcag aacgcgtttg aacacgaaaa agagaacgtc ttcgccaaaa
     4201 gctggatttg cgtcgctcac agcagcgaac tggcgaatgc caatgattat gtgacgcgtg
     4261 agatcattgg cgaaagcatc gtgctggtac gcggtcgtga taaggttttg cgcgcgttct
     4321 ataacgtgtg tccgcaccgt ggtcatcagt tgttgagcgg tgaaggaaaa gcaaaaaatg
     4381 tgattacctg cccgtatcac gcatgggcat tcaaactcga tggcaatctg gcccatgcac
     4441 gtaactgcga aaacgtcgcc aatttcgata gcgacaaagc gcaactggtt ccggtgcgtc
     4501 tggaagaata tgccggattc gtcttcatca acatggaccc caacgccacc agcgtagaag
     4561 atcagttacc cggtctgggc gcgaaagtgc tggaagcctg cccggaagtc cacgatctga
     4621 aactggcggc ccgctttacc acccgcacgt ctgccaactg gaagaacatt gtcgataact
     4681 atctcgagtg ctatcactgt ggtccggcgc atccaggttt ctccgactcc gtacaggttg
     4741 atcgttactg gcacaccatg cacggtaact ggacgctgca atacggtttc gccaaaccgt
     4801 ccgaacagtc gtttaaattt gaagagggta cggatgcggc attccacggt ttctggctgt
     4861 ggccgtgcac gatgctgaac gtcaccccga tcaaagggat gatgacggtc atttatgaat
     4921 tcccggtgga ttctgaaact accctgcaaa actacgatat ttacttcacc aatgaagagt
     4981 taaccgacga gcaaaaatcg ctgattgagt ggtatcgcga tgtgttccgt ccggaagatt
     5041 tacgtctggt tgaaagcgta cagaaagggc tgaaatcgcg tggctatcgt ggtcaggggc
     5101 gcatcatggc cgacagtagc ggtagtggca tttccgaaca tggtatcgcc catttccata
     5161 atctgctggc gcaggtgttt aaggactaat gacatcggcg gcggtatttt ccgctgctgg
     5221 gctgattttt gatggagtac agcaatatca gactatcaaa tgtttgaagt acaggtgagc
     5281 caggttgaac cccttaccga acaggtgaaa cgcttcacgc tggtggcaac cgatggcaaa
     5341 ccgctgcctg cgttcaccgg aggaagtcac gtcattgtgc agatgagcga tggtgataac
     5401 cagtacagca atgcgtattc actactgagt tcgccgcatg acacctcttg ttatcagatt
     5461 gccgttcggc tggaggaaaa ctcgcgcggc ggttcccgct ttttgcatca gcaggtaaaa
     5521 gtgggcaatc ggttaacgat ttcaacgcct aataacctgt ttgcgctaat tccctcagcc
     5581 agaaagcatc tgtttatcgc gggcggtatt ggtatcaccc ctttcctgtc gcacatggca
     5641 gagctgcaac acagcgacgt cgactggcag ctacattact gctcgcgaaa tccagaaagt
     5701 tgcgcatttc gtgatgagct agtccagcat ccgcaggctg agaaagttca cttgcatcac
     5761 tcatcaaccg gaacacgact ggaattagcg cgattattgg cggatatcga acctggcaca
     5821 cacgtttata cctgtggtcc cgaggcgcta aatgaagcgg taagaagtga agctgcgcgt
     5881 ctggacatcg ccgccgatac gctgcacttt gagcaatttg ctatcgaaga caaaaccggc
     5941 gatgccttta ccctggtgct tgcccgttcc ggaaaagagt ttgtggtgcc ggaagagatg
     6001 actattttgc aggttattga aaataataaa gccgcgaaag tggaatgttt atgtcgtgaa
     6061 ggggtatgcg gaacctgcga aacagcaata ctggaaggtg aagctgacca tcgggatcag
     6121 tattttagcg atgaagagcg tgccagccag caaagtatgt tgatctgttg ttcgcgtgcg
     6181 aagggtaaac gcctggtgtt ggatttgtag tttgccaaag ccggatgtgg cgctgagcgc
     6241 gcccagtccg gcttcggaag attttactgc ggatattcct gcaataaatt gtgtaatgct
     6301 tccgccatca gctcaccacg ccagccggaa atcagctccg gcaaattgtt ctgcggtttc
     6361 agtttccagt gccagttcag cagttggttg atttgccgac gcgatgccag caattcggcg
     6421 ctgatcttat gcgtttcgct cacgtcagta atcagcgact taatcgcttt aaacgcttta
     6481 cgataaccag gcatgtccat caggttaagc atcggctgcg gtaaggcctc ttccggcaat
     6541 gtctgcgctt tttccaccag cgtcagcagc gttttaccgt gaaagcggat ttcgctaccg
     6601 gataaaccca ggctgtccag ttcgcctaaa cttcccggca tataacgcgc gaccgaccac
     6661 aaatgctctt cacgcacgac aaagttcacc gccagatcac gctctcgcgc cttgcgcagt
     6721 cgccagtcgg ctaacagttg cagacaggcc agttggcgtg tgcgtaactg ccaggcattg
     6781 gtgatatcac gccaggcatc ttccggcgca acgacttcct gacgacgcat ttgcatcagg
     6841 cggcattcat ccagcgccgc aggtagccag ccggaggcct ccgtttctac cataagcttg
     6901 gcggtgatcg gtaacagata ccagacatcc gccgctgcgt attcacactg acgttcggtc
     6961 agcggtctgg ccagccagtc ggtgcgcgat tcactcttgt ccagcgtaac gccggaatac
     7021 tcttccacca tggaagcgaa accccatgac atcgggcgtc cgcagaaggc agaaaggatt
     7081 tgcgtgtcaa tcaagggttg tggtaattcg ccaaagacat tgaggaacac ttccagatct
     7141 tcactgcctg catggagaaa ttttgtgatg gacggatcgc gcaggatcgc tttcagcggt
     7201 gaccagtcgg tgatcccgag aggatcaatt agcgccagat gctcgccatc gaaaagttga
     7261 atcaacccca gctgcgggta ataagtgcgc gtacgaacaa attcagtatc cagggctatc
     7321 gccggaaagg cacggacggc ttcacacaaa gaagccagcg catcgtccgt ggtaatcatt
     7381 tggtaattca aattgttttc tctttagtgg gcgtcaaaaa aacgccggat taaccggcgt
     7441 ctgacgactg acttaacgct caggctttat tgtccacttt gccgcgcgct tcgtcacgta
     7501 attctcgtcg caaaattttt ccgacgttag atttcggtaa ctcatcacga aactccacca
     7561 gcttcggtac tttgtatccc gtgagctgac ggcggcaaaa agtcaccagt gactcttcgg
     7621 taagcgatgg atcttttttc actacgaaga ttttcaccgc ttcaccactg gagccggaag
     7681 gtacgccaac agccgcgact tcctgtacgc caggatgctg catgacgaca tcttcaatct
     7741 cgttgggata gacgttaaaa ccggaaacca gaatcatgtc ttttttacga tcgacaatgc
     7801 gcaggaatcc ttcttcatcc attaccgcga tgtcgccggt gtgtaaccag ccatttttga
     7861 tgatttcatc ggtagcatcg ggacgctgcc agtaacccag catcacctgc ggtcctttga
     7921 cacaaagctc acccggttga cctggtggta cttcattatc atcatcatcc accagtttgg
     7981 cttccgtcga cggcaccggc aaaccgatgc taccactatg ataatcaata tcatatgggt
     8041 taacgctgac cagcggcgca cactcggtaa ggccatagcc ttccagcaga tactgtccgg
     8101 tcagtttcac ccaacgctct gccaccactt gctgcactgg catcccaccg cctgcggaaa
     8161 gatgcagact ggagaaatcc agctgctgga actctttatt gttcagcaac gcattgaaca
     8221 aggtgttaac gccagtgata gcggtaaacg gatatttcgc taactctttt accaaccctg
     8281 gaatatcacg cgggttagtg ataagcaggt tctgcccacc cagttcgata aacagcaggc
     8341 agttaatggt cagggcaaaa atgtgataca gcggcagtgc cgttactacc agttctttac
     8401 ctggatgcag caacggccca taggtggcgt tgacctgttc caggttcgcc agcatattgc
     8461 ggtgagtcag catcgcgcct ttcgccacac ctgtggtgcc gccggtgtat tgcaggaagg
     8521 ctaaatcttc cggcaccagt tcgggtttga cgtactgcat ccggtagccg ttatgcagtg
     8581 cgctacgaaa tgaaatggca tctggcagat ggtatttcgg caccaaacgc ttgatgtatt
     8641 taacaacgaa attgactacc gtgccttttg ccgtagatag ctgatcgccc atacgggtca
     8701 gaattacgtg ctgaacggcg gttttatcaa ccactttttc cagtgtgtga gcaaagttag
     8761 acacgataac aaccgccgat gcgccgctat cgttaagctg atgctcaagc tcacgcgggg
     8821 tatacaacgg gttaacgttt acgacgatca tcccggcacg caaaatgcca aacagcgcca
     8881 ccggatattg caataaatta ggcatcatca acgcaacgcg atcgcctttc ttcagcccca
     8941 ccccttgttg caaataagcg gcaaacgcgc gactgcgttc ttccagcttg cggaaggtca
     9001 tcacctcccc catattcaca aacgcaggct gatcggcgta gcgcgcgacc gactgctcaa
     9061 acatatctac cagagattga taacggtcag ggttgatctc cgtcggaacg tccgcgggat
     9121 aacggttaag ccaaaccttc ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg
     9181 caaccccaaa tgatatacat gccgttaaca taatattaac tcatcatacc agcttgataa
     9241 ttacccaacg aaaaggttgc gaagcgcgtc actatttatt tttatcttta ccgtaagaat
     9301 gcagaaacag cggaccagcc gctgtttctt tttctttaaa aacaagcaat tattcagtta
     9361 caactgtttg tactctcgcg ggaccgggat tataccagcc ccatccgccg tagccatagg
     9421 gccagccacg accgccataa aaccatggat caatcggctg aggcggcata atcacctgct
     9481 gggttaaatg ccaacgtttg taacccgtta cttgcatcac cataaattta tagggcgtat
     9541 tgccgatttt gccgtcaacc gcaccagtga ttggcccgac taccgtaacc agttgtccac
     9601 ggaaatccac cgggtccaga aaaccgttca catcggcata aatgcgaccg cgagaaggtt
     9661 ctcccagcgt cggtctggct ccgctgtcca gcggtacggt agcaatttcc aggcgggttt
     9721 tcccttgctg gttttgtacc gcaaccactt tgccaccaaa gcgtgcctcc tgaccaacgt
     9781 acagctgcgg cgcactcatc acccgaacta aatcttgttg cggcgtggga ctgcggcctt
     9841 taatggcgtc cggcacagtg acacaaccgc tcagcattag cgcaaacgta cctgccagta
     9901 tgcctttgat aacattcttt tgaaccgcca tgatgcgact cctttttttc tcagagcata
     9961 ctcttaagat tcattctttg cccggaagtt tcttccatgc gacgttgtta cgtaaataaa
    10021 ccggttcggc atgttccacc gccaccgttt taccctcggc aaatatctga cacgcaatcg
    10081 gcagcatatc ttccgtcgct ggcagtaaaa cttctccgtc gcgcaaaacc agcccgctct
    10141 ctttaccgag atccggccag gcttgccagc ccgtccctac cgttacccat tcgccggaaa
    10201 gctgttgcat tcgttcatga acgagttcgg gtttgagtac ggcttcggtt tcttcaccgc
    10261 gccagatacc gttttcatca cgctgatatt cggcccagta aacttcgccc atccgcgcat
    10321 caatggcagc cagcacgcgg gttgcgccgt ttttgcgcca cgccccttgc gccattgtca
    10381 ttagcgtgga gacgccaatc atcggcagct ctgcgccaag tgccagccct tgcgcaatgc
    10441 caataccaat gcgcacgccg gtaaagctac cggggccgcg cccgtaagcc agagcgttaa
    10501 tatcagtcag ggaagttccg ctggtggtca ggatatcctg caccatcggt aagattcgtt
    10561 gagtatgttc acgagggcaa agctcaaaat gagcgttgac agtaccgtcg ttccacaggg
    10621 cgacagagca ggcctctgtc gcggtatcga tagccagaat tcgcatgggt cttcgtgctt
    10681 agatcaataa aaaggcgcgc atcataccat actccgtaac aaattacctg gaggatggta
    10741 tcgcaaggaa acgaaccgca cgggcaatgt cacgggtgcg cggcgcgggc ggcagactgg
    10801 cgagaaacgt cgcgccgtaa gggcgcatca ccagccgatt gtcacaaatc accaaaaccc
    10861 cgcgatcgtc ggcgtcgcga atcagtcgcc ctaccccctg cttgagagta atcaccgcat
    10921 ccggcagttg tacttcatcg aacgggtcgc caccgcgcaa acggcaatct tccatgcgcg
    10981 cttttaacag cgggtcatcc ggtgaggtaa acggcaactt gtcgataatc accaatgaca
    11041 atgtatcgcc acgcacgtcc accccttccc agaaactact ggttgccaca agaagcgcgt
    11101 taccggcgct gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa
    11161 cgggaagcgt catggtagcg cggaactgct cggccagatc acgcatcatg gcgtgcgagg
    11221 tacaaagcat aaaacaacga ccgttgttag cttcgatgat cggtcgcagc attgccgcta
    11281 actggcgagc agaacctggc tggttggttt gcggcagatt gcgcggcaca cagagtaacg
    11341 cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa
    11401 gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc
    11461 agctaccagg cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga
    11521 gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga
    11581 tctcttttag ccgcttcagc cgtgtgcgat acaacgtagc gcgctcaaat gccgcatcca
    11641 gcaaggcgga acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat
    11701 cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc
    11761 gataacctgg ctcaccgagt tgcagacgaa aatcctgcgc actctgggca agacgatcag
    11821 cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt
    11881 ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt
    11941 ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttccgggatc agttcgccaa
    12001 atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat
    12061 cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt
    12121 cgctgccaag acagttgtcg ttggtgctgg taaccagcgg ccacgcctgt gaatcttccg
    12181 ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca
    12241 ggatcacatc gcttaagatt tgtaccggca gatcgccccc cgccagcgcc tgctgttcga
    12301 gacgttcgag gcagaggtag tttgagcgcc ctttcagcag cgccacgttg cccgtatatt
    12361 tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg
    12421 agccggtcga gataatgact ttctttttcg cccgcagcgc aggagccagg taagcgtagg
    12481 ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg
    12541 tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg
    12601 ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacgggg
    12661 attatgtcag gatgagggcg ctttcgccag ttgaagtggt gacggcaacc tcacattgtg
    12721 gcagtctttg cagcacgaaa atggaagtat aatgaggaca aaatgatgac tatcgttcgt
    12781 atcgatgctg aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact
    12841 ggtgtaccgg aaaacctcga cgccgatgct tttgagcaaa ccgccaacac gctggc
//
LOCUS       DAJTYD010000105        12847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTYD010000105 DAJTYD010000000
VERSION     DAJTYD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.105"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..235)
                     /gene="rpsT"
                     /locus_tag="OO585_002691"
     CDS             complement(<1..235)
                     /gene="rpsT"
                     /locus_tag="OO585_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6630227.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKAN"
     gene            338..556
                     /locus_tag="OO585_002692"
     CDS             338..556
                     /locus_tag="OO585_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6630228.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            564..1505
                     /gene="ribF"
                     /locus_tag="OO585_002693"
     CDS             564..1505
                     /gene="ribF"
                     /locus_tag="OO585_002693"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6630229.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1548..4364
                     /gene="ileS"
                     /locus_tag="OO585_002694"
     CDS             1548..4364
                     /gene="ileS"
                     /locus_tag="OO585_002694"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6630230.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4364..4858
                     /gene="lspA"
                     /locus_tag="OO585_002695"
     CDS             4364..4858
                     /gene="lspA"
                     /locus_tag="OO585_002695"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6630231.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            4927..5376
                     /gene="fkpB"
                     /locus_tag="OO585_002696"
     CDS             4927..5376
                     /gene="fkpB"
                     /locus_tag="OO585_002696"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6630232.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5378..6328
                     /gene="ispH"
                     /locus_tag="OO585_002697"
                     /gene_synonym="lytB"
     CDS             5378..6328
                     /gene="ispH"
                     /locus_tag="OO585_002697"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6630233.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6394..7308
                     /gene="rihC"
                     /locus_tag="OO585_002698"
     CDS             6394..7308
                     /gene="rihC"
                     /locus_tag="OO585_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6630234.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7475..8296
                     /gene="dapB"
                     /locus_tag="OO585_002699"
     CDS             7475..8296
                     /gene="dapB"
                     /locus_tag="OO585_002699"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6630235.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            8751..9899
                     /gene="carA"
                     /locus_tag="OO585_002700"
     CDS             8751..9899
                     /gene="carA"
                     /locus_tag="OO585_002700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6630236.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            9917..>12847
                     /gene="carB"
                     /locus_tag="OO585_002701"
     CDS             9917..>12847
                     /gene="carB"
                     /locus_tag="OO585_002701"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6630237.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGT"
BASE COUNT         2959 a         3251 c         3651 g         2986 t
ORIGIN      
        1 ggttagcctt atgacgtgca gctttgtttt tgtggatcag acctttagca gcctgacggt
       61 ccacgatcgg ttgcatttcg ttaaatgctt tctgtgcagc agctttgtcg ccagcttcga
      121 tagctgcgta tactttcttg atgaaagtac gcatcataga gcgacggctt gcgttgtgct
      181 tacgagcctt ttcagactga atggcgcgct tcttagctga tttgatatta gccaaggtcc
      241 aactcccaaa tgtgttctat atggacaatt caaaggccga ggaatatgcc cttttagcct
      301 tcttttgtca atggatttgt gcaaataagc gccgttaatg tgccggcact cgttacgtag
      361 tgatggcgca ggattctacc agcttgcggg gtgtgaatac agcttttccg cgataaaaat
      421 tgcagcaggc ggtcactttc ttcccgtgat ttgcgccatg gcaatgaaaa gccacttctt
      481 tctgatttcg gtactcaatc gccggttaac cttgaccgct gtacaaggta tactcggacg
      541 attttcactg ttttgagcca gacatgaagc tgatacgcgg catacataat ctcagccagg
      601 ccccgcaaga agggtgtgtg ctgactattg gtaatttcga cggcgtgcat cgcggtcatc
      661 gcgcgctgtt acagggcttg caggaagaag ggcgcaagcg caacttaccg gtgatggtga
      721 tgctttttga acctcaacca ctggaactgt ttgctaccga taaagccccg gcaagactga
      781 cccggctgcg ggaaaaactg cgttaccttg cagagtgtgg cgttgattac gtgctgtgcg
      841 tgcgtttcga caggcgtttc gcggcgttaa ccgcgcaaaa tttcatcagc gatcttctgg
      901 tgaagcattt gcgcgtaaaa tttcttgccg taggggatga tttccgcttt ggcgctggtc
      961 gtgaaggcga tttcttgtta ttacagaaag ctggcatgga atacggcttc gatatcacta
     1021 gtacgcaaac tttttgcgaa ggtggcgtgc gtattagcag caccgctgtg cgtcaggcgc
     1081 tggcggatga caatctggct ctggcagaaa gtttactggg gcacccgttt gccatctccg
     1141 ggcgtgtagt ccacggtgat gaattagggc gcactatagg tttcccgacg gcgaatgtac
     1201 cgctacgccg tcaggtttcc ccggtgaaag gggtttatgt ggtagaagtg ctgggcctcg
     1261 gtgaaaagcc gttacccggc gtggcaaaca tcggaacacg cccaacggtt gccggtattc
     1321 gccagcagct ggaagtgcat ttgttagatg ttgcaatgga cctttacggt cgccatatac
     1381 aagtagtgct gcgtaaaaaa atacgcaatg agcagcgatt tgcgtcgctg gacgaactga
     1441 aagcgcagat tgcgcgtgat gaattaaccg cccgcgaatt ttttgggcta acaaaaccgg
     1501 cttaagcctg ttatgtaatc aaaccgaaac acggaaccga gaatctgatg agtgactata
     1561 aatcaaccct gaatttgccg gaaacagggt tcccgatgcg tggcgatctc gccaagcgcg
     1621 aaccgggaat gctggcgcgt tggactgatg atgatctgta cggcatcatc cgtgcggcta
     1681 aaaaaggcaa aaaaaccttc attctgcatg atggccctcc ttatgcgaat ggcagcattc
     1741 atattggtca ctcggttaac aagattctga aagacattat cgtgaagtcc aaagggcttt
     1801 ccggttatga ctcgccgtat gtgcctggct gggactgcca cggtctgccg atcgagctga
     1861 aagtagagca agaatacggt aagccgggtg agaaattcac cgccgccgag ttccgcgcca
     1921 agtgccgcga atacgcggcg acccaggttg acggtcaacg caaagacttt atccgtctgg
     1981 gcgtgctggg cgactggtcg cacccgtacc tgaccatgga cttcaaaact gaagccaaca
     2041 tcatccgcgc gctgggcaaa atcatcggca acggtcacct gcacaaaggc gcgaagccgg
     2101 tacactggtg cgttgactgc cgttctgcgc tggcggaagc ggaagttgag tattacgaca
     2161 aaacttctcc gtccatcgac gttgcttttc aggcggtcga tcaggatgca ctgaaagcaa
     2221 aatttgccgt aagcaacgtt aacggcccaa tctcgctggt gatctggacc actacgccgt
     2281 ggactctgcc tgcgaaccgc gcaatctcta ttgcacctga tttcgactat gcgctggtgc
     2341 agatcgacgg tcaggccgtg attctggcga aagatctggt tgaaagcgta atgcagcgta
     2401 tcggcgtgac cgattacacc attctcggca cggtaaaagg tgcggagctt gagttgctgc
     2461 gctttaccca tccgtttatg ggcttcgacg ttccggcaat cctcggcgat cacgttaccc
     2521 tggatgccgg taccggtgcc gttcacaccg cgcctggcca cggcccggac gactatgtga
     2581 tcggtcagaa atacggcctg gaaaccgcta acccggttgg cccggacggc acttatctgc
     2641 cgggcactta tccgacgctg gatggcgtga acgtcttcaa agcgaacgac atcgtcgttg
     2701 cgctgctgca ggaaaaaggc gcgctgctgc acgttgagaa aatgcagcac agctatccgt
     2761 gctgctggcg tcacaaaacg ccgatcatct tccgcgcgac gccgcagtgg ttcgtcagca
     2821 tggatcagaa aggtctgcgt gcgcagtcac tgaaagagat caaaggcgtg cagtggatcc
     2881 cggactgggg ccaggcgcgt atcgagtcga tggttgctaa ccgtcctgac tggtgtatct
     2941 cccgtcagcg cacctggggc gtaccgatgt cactgttcgt gcacaaagac acggaagagc
     3001 tgcatccgcg tactctcgaa ctaatggaag aagtggcaaa acgcgttgaa gttgacggca
     3061 tccaggcgtg gtgggatctt gatgcgaaag agatcctcgg cgatgaagct gatcagtacg
     3121 tgaaagtgcc ggacacattg gatgtatggt ttgactccgg atctacccac tcttctgttg
     3181 ttgacgtgcg tccggaattt gccggtcacg cagcggacat gtatctggaa ggttctgacc
     3241 agcaccgtgg ttggttcatg tcttccctaa tgatctccac cgcgatgaaa ggcaaagcgc
     3301 cgtatcgtca ggtactgacc cacggcttta ccgtggatgg tcagggccgc aagatgtcta
     3361 aatccatcgg caataccgtt tcgccgcagg atgtgatgaa caaactgggc gcggatattc
     3421 tgcgtctgtg ggtggcatca accgactaca ccggtgaaat ggccgtttct gacgagatcc
     3481 tgaaacgtgc tgccgatagc tatcgtcgta tccgtaacac cgcgcgcttc ctgctggcaa
     3541 acctgaacgg ttttgatcca gcaaaagata tggtgaaacc ggaagagatg gtggtactgg
     3601 atcgctgggc cgtaggttgt gcgaaagcgg cacaggaaga catcctcaag gcgtacgaag
     3661 catacgattt ccacgaagtg gtacagcgtc tgatgcgctt ctgctccgtt gagatgggtt
     3721 ccttctacct cgacatcatc aaagaccgtc agtacaccgc caaagcggac agtgtggcgc
     3781 gtcgtagctg ccagactgcg ctgtatcaca tcgcagaagc gctggtgcgc tggatggcac
     3841 caatcctctc cttcaccgct gatgaagtgt ggggctacct gccgggcgaa cgtgaaaaat
     3901 acgtcttcac cggtgagtgg tacgaaggcc tgtttggcct ggcagacagc gaagcgatga
     3961 acgatgcgtt ctgggacgag ctgttgaaag tgcgtggcga agtgaacaaa gtcattgagc
     4021 aagcgcgtgc cgacaagaaa gtgggtggct cgctggaagc ggcagtaacc ttgtatgcag
     4081 aaccggaact ggcggcgaaa ctgaccgcgc tgggcgatga attacgattt gtcctgttga
     4141 cctccggcgc taccgttgca gactatcacg acgcacctgc tgatgctcag cagagcgaag
     4201 tactcaaagg gctgaaagtc gcgttgagta aagccgaagg tgagaagtgc ccacgctgct
     4261 ggcactacac ccaggatgtc ggcaaggtgg cggaacacgc agaaatctgc ggccgctgtg
     4321 tcagcaacgt cgccggtgac ggtgaaaaac gtaagtttgc ctgatgagtc aatcgatctg
     4381 ttcaacaggg ctacgctggc tgtggctggt ggtagtcgtg ctgattatcg atctgggcag
     4441 caaatacctg atcctccaga actttgctct gggggatacg gtcccgctgt tcccgtcgct
     4501 taatctgcat tatgcgcgta actatggcgc ggcgtttagt ttccttgccg atagcggcgg
     4561 ctggcagcgt tggttctttg ccggtattgc gattggtatt agcgtgatcc tggcagtgat
     4621 gatgtatcgc tcgaaggcca cgcagaagct aaacaatatc gcttacgcgc tgattattgg
     4681 cggcgcgctg ggcaacctgt tcgaccgcct gtggcacggc ttcgttgtcg atatgatcga
     4741 cttctacgtc ggcgactggc acttcgccac cttcaacctt gccgatactg ccatctgtgt
     4801 cggtgcggca ctgattgtgc tggaaggttt tttgccttct agagcgaaaa aacaataata
     4861 aaccctgccg gataaggcgt ttacgccgca tccggcaaaa atcctcaaat ataagagcaa
     4921 acctgcatgt ctgaatctgt acagagcaat agcgccgtcc tggtgcactt cacgctaaaa
     4981 ctcgacgatg gcaccaccgc cgagtctacc cgcaacaacg gtaaaccggc gctgttccgc
     5041 ctgggtgatg cttctctttc tgaagggctg gagcaacacc tgttggggct gaaagtgggc
     5101 gataaaacca ccttctcgtt ggagccagat gcggcgtttg gcgtgccgtc accggacctg
     5161 attcagtact tctcccgccg tgaatttatg gatgcaggcg agccagaaat tggcgcaatc
     5221 atgcttttta ccgcaatgga tggcagtgag atgcctggcg tgatccgcga aattaacggc
     5281 gactccatta ccgttgattt caaccatcct ctggccgggc agaccgttca ttttgatatt
     5341 gaagtgctgg aaatcgatcc ggcattggag gcgtaacatg cagatcctgt tggccaaccc
     5401 gcgtggtttt tgtgccgggg tagaccgcgc tatcagcatt gttgaaaacg cgctggccat
     5461 ttacggcgca ccgatatatg tccgtcacga agtggtacat aaccgctatg tggtcgatag
     5521 cttgcgtgag cgtggggcta tctttattga gcagattagc gaagtaccgg acggcgcgat
     5581 cctgattttc tccgcacacg gtgtttctca ggcggtacgt aacgaagcaa aaagtcgcga
     5641 tttgacggta tttgacgcga cctgtccgct ggtgaccaaa gtgcatatgg aagtcgcccg
     5701 cgccagtcgc cgtggcgaag aatctattct catcggtcac gccgggcacc cggaagtgga
     5761 agggacaatg ggccagtaca gtaacccgga agggggaatg tatctggtcg aatcgccgga
     5821 cgatgtgtgg aaactgacgg tcaaaaacga agagaagctc tcctttatga cccagaccac
     5881 gctgtcggtg gatgacacgt ctgatgtgat cgacgcgctg cgtaaacgct tcccgaaaat
     5941 tgtcggtccg cgcaaagatg acatctgcta cgccacgact aaccgtcagg aagcggtacg
     6001 cgccctggca gaacaggcgg aagttgtgtt ggtggtcggt tcgaaaaact cctccaactc
     6061 caaccgtctg gcggagctgg cccagcgtat gggcaaacgc gcgtttttga ttgacgatgc
     6121 gaaagacatc caggaagagt gggtgaaaga ggttaaatgc gtcggcgtga ctgcgggcgc
     6181 atcggctccg gatattctgg tgcagaatgt ggtggcacgt ttgcagcagc tgggcggtgg
     6241 tgaagccatt ccgctggaag gccgtgaaga aaacattgtt ttcgaagtgc cgaaagagct
     6301 gcgtgtcgat attcgtgaag tcgattaagt cattagcagc ctaagttatg cgaaaatgcc
     6361 ggtcttgtta ccggcatttt ttatggagaa aacatgcatt tacctatctt cctcgatact
     6421 gaccccggca ttgacgatgc cgtcgccatt gccgccgcga tttttgcacc cgaactcgac
     6481 ctgcaactga tgaccaccgt cgcgggtaat gtctcggttg agaaaaccac ccgcaacgcc
     6541 ctgcaactgc tgcatttctg gaatgcggag attccgctcg cccagggagc cgctgtgccg
     6601 ctggtacgcg caccgcgtga tgcggcatct gtgcacggcg aatcgggaat ggctggctac
     6661 gactttgttg agcacaaccg aaagccgctc gggataccag cgtttctggc gattcgggat
     6721 gccctgatgc gtgcaccaga gcccgttacc ctggtggcca tcggcccgtt aaccaatatt
     6781 gcgctgttac tttcacaatg cccggaatgt aaaccgtata ttcgtcgtct ggtgatcatg
     6841 ggcggttctg ctggtcgcgg caactgcaca ccaaacgccg agtttaatat cgctgccgat
     6901 ccagaagcgg ctgcctgtgt cttccgtagc ggcatcgaaa tcgtcatgag cggtctggat
     6961 gtcaccaatc aggcaatatt aacccctgac tatctcgcta ctttgccgga gttgaatcgc
     7021 accggaaaaa tgctccacgc cctgtttagc cactaccgta gcggcagtat gcaaagtgga
     7081 ctgcgtatgc acgatctttg cgctattgcc tggctggtgc gcccggaatt gttcactctc
     7141 aaaccgtgtt ttgtggcagt ggaaactcag ggcgaattta cctcaggcac gacggtggtt
     7201 gatatcgacg gttgcctggg caaaccagcc aatgtaaagg tggcattgga tctggatgtg
     7261 aaaggcttcc agcagtgggt ggctgaggtg ctggctctgg cgtcgtaacc tgtcacatgt
     7321 tatcggcatg cagtcattca tcgactcatg cctttcactg atatccctcc ctgtttatca
     7381 ttaatttcta attatcagcg tttttggctg gcggcgtagc gatgcgctgg ttactctgaa
     7441 aacggtctat gcaaattaac aaaagagaat agctatgcat gatgcaaaca tccgcgttgc
     7501 catcgcggga gccggggggc gtatgggccg ccagttgatt caggcggcgc tggcattaga
     7561 aggcgtgcag ctgggcgcgg cgctggagcg cgaaggatct tctttactgg gcagcgacgc
     7621 cggtgagctg gctggtgcag ggaaaacagg cgttaccgtg caaagcagcc tcgatgcggt
     7681 aaaagatgat tttgatgtgt ttatagattt tacccgtccg gaaggtacgc tgaaccatct
     7741 cgctttttgt cgccagcatg gcaaagggat ggtgatcggc actacggggt ttgacgaagc
     7801 cggtaaacag gcgattcgtg acgctgccgc cgatattgcg attgtctttg ccgccaattt
     7861 tagcgttggc gttaacgtca tgcttaagct gctggagaaa gcagccaaag tgatgggtga
     7921 ctacaccgat atcgaaatta ttgaagcaca tcatagacat aaagttgatg cgccgtcagg
     7981 caccgcactg gcaatgggag aggcgatcgc ccacgccctt gataaagatc tgaaagattg
     8041 cgcggtctac agtcgtgaaa gccacaccgg tgaacgtgtg cctggcacca ttggttttgc
     8101 caccgtgcgt gcaggtgaca tcgttggcga acataccgcg atgtttgccg atattggcga
     8161 gcgtctggag atcacccata aggcgtccag ccgcatgaca tttgctaacg gcgcggtaag
     8221 atcggctttg tggttgagtg gtaaggaaag tggtcttttt gatatgcgag atgtgcttga
     8281 tctcaatagt ttataaccac aaaatatttg ttatggtgca aaaataacac atttaattta
     8341 ttgattataa agggctttaa ttttggccct tttattttta gtgttatgtt tttaaattgt
     8401 ctataagtgc caaaaattac atgttttgtc ttctgttttt gttgttttaa tgtaaaattt
     8461 gaccatttgg tccacttttc cctgctcgct tttatttcat gcaatcttct tgctgtgcaa
     8521 gcgttttcca gaacaggtta tatgatcttt ttgtcgctta atgcctgtaa aacatgcatg
     8581 agccacaaaa taatataaaa aatcccgcca ttaagttgac ttttagcgcc catatctcca
     8641 gaatgccgcc gtttgccaga aattcgtcgg taagcagatt tgcattgatt tacgtcatca
     8701 ttgtgaatta atatgcaaat aaagtgagtg aatattctct ggagggtgtt ttgattaagt
     8761 cagcgctatt ggttctggaa gacggaaccc agtttcacgg tcgggccata ggggcaacag
     8821 gttcggcggt tggggaagtc gttttcaata cttcaatgac cggttatcaa gaaatcctca
     8881 ctgatccttc ctattctcgt caaatcgtta ctcttactta tccccatatt ggcaatgtcg
     8941 gcactaatga cgccgatgaa gaatcttctc aggtacatgc acaaggtctg gtgattcgcg
     9001 acctgccgct gattgccagc aacttccgta ataccgaaga cctctcttct tacctgaagc
     9061 gccataacat cgtggcgatt gccgatatcg atacccgtaa gctgacgcgt ttactgcgcg
     9121 agaaaggcgc acagaatggc tgcattatcg caggcgataa cccggatgcg gcgttggcgt
     9181 tagaaaaagc ccgcgcgttc ccaggtctga atggcatgga tctggcaaaa gaagtgacca
     9241 ccgcagaagc ctatagctgg acacaaggga gctggacgtt gaccggcggc ctgccagaag
     9301 cgaaaaaaga agacgagctg ccgttccacg tcgtggctta tgattttggt gccaagcgca
     9361 acatcctgcg gatgctggtg gatagaggct gtcgcctgac catcgttccg gcgcaaactt
     9421 ctgcggaaga tgtgctgaaa atgaatccag acggcatctt cctctccaac ggtcctggcg
     9481 acccggcccc gtgcgattac gccattaccg ccatccagaa attcctcgaa accgatattc
     9541 cggtattcgg catctgcctc ggtcatcagc tgctggcgct ggcgagcggt gcgaagactg
     9601 tcaaaatgaa atttggtcac cacggcggca accatccggt taaagatgtg gagaaaaacg
     9661 tggtgatgat caccgcccag aaccacggtt ttgcggtgga tgaagcaaca ttacctgcaa
     9721 acctgcgtgt cacgcataaa tccctgttcg acggtacgtt acagggcatt catcgcactg
     9781 ataaaccggc gttcagcttc cagggtcacc cggaagccag ccctgggcca cacgacgcag
     9841 cgccgttgtt cgaccacttt atcgagttaa ttgagcagta ccgtaaaacc gctaagtaat
     9901 caggagtaaa agagccatgc caaaacgtac agatataaaa agtatcctga ttctgggtgc
     9961 gggcccgatt gttatcggtc aggcgtgtga gtttgactac tctggcgcgc aagcatgtaa
    10021 agccctgcgc gaagagggtt accgcgtcat tctggtgaac tccaacccgg cgaccatcat
    10081 gaccgacccg gaaatggccg atgcgaccta catcgagccg attcactggg aagtagtacg
    10141 caagattatt gaaaaagagc gcccggacgc ggtgctgcca acgatgggcg gtcagacggc
    10201 gctgaactgc gctctggagc tggagcgtca gggcgtgttg gaagagttcg gcgtgactat
    10261 gattggtgcg accgccgatg cgattgataa agcagaagac cgccgtcgtt tcgacgtagc
    10321 gatgaagaaa attggtctgg aaaccgcgcg ttccggtatc gcacacacga tggaagaagc
    10381 gctggcggtt gccgctgacg tgggcttccc gtgcattatt cgcccatcct ttaccatggg
    10441 cggtagcggc ggcggtatcg cttataaccg cgaagagttt gaagaaattt gcgcccgcgg
    10501 tctggatctc tccccaacca aagagctgct gattgatgag tcgctgatcg gctggaaaga
    10561 gtacgagatg gaagtggtgc gtgataaaaa cgacaactgc atcatcgtct gctctatcga
    10621 aaactttgat gcgatgggca tccataccgg tgactccatc actgtcgcgc cagcccaaac
    10681 gctgaccgac aaagaatatc aaatcatgcg taacgcctcg atggcggtgc tgcgtgaaat
    10741 cggtgttgaa accggtggtt ccaatgtcca gtttgcggtg aacccgaaaa acggtcgcct
    10801 gattgttatc gaaatgaacc cacgcgtgtc ccgttcttcg gcgctggcgt cgaaagcgac
    10861 cggtttcccg attgctaaag tggcggcgaa actggcggtg ggttacaccc tcgacgaact
    10921 gatgaacgac atcactggcg gacgtactcc ggcctccttc gagccgtcca tcgactatgt
    10981 ggttactaaa attcctcgct tcaacttcga aaaattcgct ggtgctaacg accgtctgac
    11041 cactcagatg aaatcggttg gcgaagtgat ggcgattggt cgcacgcagc aggaatccct
    11101 gcaaaaagcg ctgcgcggcc tggaagtcgg tgcgactgga ttcgacccga aagtgagcct
    11161 cgatgacccg gaagcgttaa ccaaaatccg tcgcgaactg aaagatgctg gcgcagagcg
    11221 tatctggtac atcgccgatg ccttccgtgc gggcctgtct gtggacggcg tgttcaacct
    11281 gaccaatatt gaccgctggt tcctggtaca gattgaagaa ctggtgcgtc tggaagagaa
    11341 agtagcggaa gtgggcatca ctggcctgaa cgctgaattc ctgcgccagc tgaaacgcaa
    11401 aggctttgcc gatgcgcgtc tggcaaaact ggcgggcgtg cgcgaagcgg aaatccgcaa
    11461 gctgcgcgac cagtatgacc tgcacccggt ttataagcgc gtggatacct gtgcggcaga
    11521 gttcgccacc gacaccgctt acatgtactc cacttatgaa gaagagtgcg aagcgaatcc
    11581 gtctaccgac cgtgaaaaaa tcatggttct cggcggcggc ccgaaccgta tcggtcaggg
    11641 tatcgaattc gactactgct gtgtacacgc ctcgctggcg ctgcgcgaag acggttacga
    11701 aaccattatg gttaactgta acccggaaac cgtctccacc gactacgaca cttccgatcg
    11761 cctctacttc gagccggtaa ctctggaaga tgtgctggaa atcgtgcgta ttgagaagcc
    11821 gaaaggcgtt atcgttcagt acggcggtca gaccccgctg aaactggcgc gcgcgctgga
    11881 agctgctggc gtaccggtta tcggcaccag cccggatgct atcgaccgtg cagaagaccg
    11941 tgaacgcttc cagcatgcgg ttgagcgtct gaaactgaaa caaccggcga acgccaccgt
    12001 taccgctatt gaaatggcgg tagagaaggc gaaagagatt ggctacccgc tggtggtacg
    12061 tccgtcttac gttctcggcg gtcgggcgat ggaaatcgtc tatgacgaag ctgacctgcg
    12121 tcgctacttc cagacggcgg tcagcgtgtc taacgatgcg ccagtgttgc tggatcactt
    12181 ccttgatgac gcggtagaag ttgacgtgga tgccatctgc gacggcgaaa tggtgctgat
    12241 tggcggcatc atggagcaca tcgagcaggc gggcgtgcac tccggtgact ccgcatgttc
    12301 tctgccagcc tacaccttaa gtcaggaaat tcaggatgtg atgcgccagc aggtgcagaa
    12361 actggccttc gaattgcagg tgcgcggtct gatgaacgtg cagtttgcgg tgaaaaacaa
    12421 cgaagtctac ctgattgaag ttaacccgcg tgcggcgcgt accgttccgt tcgtctccaa
    12481 agccaccggc gtaccgctgg caaaagtggc ggcgcgtgtg atggctggca aatcgctggc
    12541 tgagcagggc gtaaccaaag aagttatccc gccgtactac tcggtgaaag aagtggtgct
    12601 gccgttcaat aaattcccgg gtgttgaccc gctgttaggg ccagaaatgc gctccaccgg
    12661 ggaagtgatg ggcgtgggcc gcaccttcgc tgaagcgttt gccaaagcgc agctgggcag
    12721 caactccacc atgaagaaac acggtcgtgc gttgctttcc gtgcgcgaag gcgataaaga
    12781 acgcgtggtg gacctggcgg caaaactgct gaaacagggc ttcgagctgg atgcgaccca
    12841 cggcacg
//
LOCUS       DAJTYD010000106        12671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTYD010000106 DAJTYD010000000
VERSION     DAJTYD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.106"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..125
                     /locus_tag="OO585_002702"
     CDS             <1..125
                     /locus_tag="OO585_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6630238.1"
                     /translation="GVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPES"
     gene            complement(189..680)
                     /locus_tag="OO585_002703"
     CDS             complement(189..680)
                     /locus_tag="OO585_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6630239.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(782..1336)
                     /gene="ycdY"
                     /locus_tag="OO585_002704"
     CDS             complement(782..1336)
                     /gene="ycdY"
                     /locus_tag="OO585_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6630240.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1360..2097)
                     /gene="ycdX"
                     /locus_tag="OO585_002705"
     CDS             complement(1360..2097)
                     /gene="ycdX"
                     /locus_tag="OO585_002705"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6630241.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2152..3090)
                     /gene="ghrA"
                     /locus_tag="OO585_002706"
     CDS             complement(2152..3090)
                     /gene="ghrA"
                     /locus_tag="OO585_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6630242.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3324..3411
                     /locus_tag="OO585_002707"
     tRNA            3324..3411
                     /locus_tag="OO585_002707"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3358..3360,aa:Ser,seq:gga)
     gene            complement(3561..4403)
                     /locus_tag="OO585_002708"
     CDS             complement(3561..4403)
                     /locus_tag="OO585_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6630243.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKTIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(4488..4685)
                     /locus_tag="OO585_002709"
     CDS             complement(4488..4685)
                     /locus_tag="OO585_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630244.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(4697..5188)
                     /locus_tag="OO585_002710"
     CDS             complement(4697..5188)
                     /locus_tag="OO585_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630245.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(5185..5559)
                     /locus_tag="OO585_002711"
     CDS             complement(5185..5559)
                     /locus_tag="OO585_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6630246.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(5606..5980)
                     /locus_tag="OO585_002712"
     CDS             complement(5606..5980)
                     /locus_tag="OO585_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6630247.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(6143..6364)
                     /locus_tag="OO585_002713"
     CDS             complement(6143..6364)
                     /locus_tag="OO585_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6630248.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6427..6903)
                     /locus_tag="OO585_002714"
     CDS             complement(6427..6903)
                     /locus_tag="OO585_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6630249.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(6919..7392)
                     /locus_tag="OO585_002715"
     CDS             complement(6919..7392)
                     /locus_tag="OO585_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6630250.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(7734..8552)
                     /locus_tag="OO585_002716"
     CDS             complement(7734..8552)
                     /locus_tag="OO585_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6630251.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(8707..8865)
                     /locus_tag="OO585_002717"
     CDS             complement(8707..8865)
                     /locus_tag="OO585_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6630252.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(8936..11782)
                     /gene="ag43"
                     /locus_tag="OO585_002718"
     CDS             complement(8936..11782)
                     /gene="ag43"
                     /locus_tag="OO585_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR6630253.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            complement(12154..>12671)
                     /locus_tag="OO585_002719"
     CDS             complement(12154..>12671)
                     /locus_tag="OO585_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR6630254.1"
                     /translation="WLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPCHEW
                     DMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHA
                     ASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVS
                     VARAVWNWIFF"
BASE COUNT         2942 a         3434 c         3279 g         3016 t
ORIGIN      
        1 caggggtcat tttcctgatt aatgatggta tcgaccgtgg tctgtgggat ttgcaaaata
       61 aagcagaacg gcagaatgac attctggtga aataccgcca tatgtcggtt ccaccggaat
      121 cctgacagat aaaaaagccg gaattatcat ccggcatttt actgtttaag cgaaagcgtg
      181 agtgcccctc acgctttatt tttgatttcc tgctcacggt gagccgtctt tgcactacgc
      241 gccgccagcc acaggccgct gtttttcatt gcgtaaccaa ataccagccc aagcgccagc
      301 gaaggtaaca ccagtttcca gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac
      361 gtccccggaa caaatgaaag cagcagctgt ttggcctgaa tacacatcag aaacgccaca
      421 ataccggtta tgacataacc gagaatttcc agatgtggtg ccagtgcact accgtaaata
      481 atgaccatcg cccacaccac gccactaagc agggttgcag cggagatcgc cagccctttc
      541 agcccacctt gcgggcaggc aaagtaggcc gtacagccca ggaagcccgc ccagcttagt
      601 aagcccaggg aaacagccac ccatccccag ataccggaga gaatgcctgt tgtgattgca
      661 atagagagaa gtatgttcat gccgcacatg atagcagaaa acgcggccat gaatgagatc
      721 gagcacacat tttatgcaac aacaaattat ctgttgcagt tatgagtgac gatgatcaca
      781 tttactcttc agaatcttct tccagctcgt cccacattgc actaatggca tcacgggtta
      841 gcggtgccat ggtgcgccag aaaggcgtgg ttgcatgggc ctccactttg ccgaggaacg
      901 caccacacca gggtaacaga tactcactga acagtgtttc cagtgcttcg ctctcatctt
      961 ccgttgactg atcttccatc caggaagccg caagcagcaa tgtgccgatg tgatcggctg
     1021 gcgtatccgc cagtggcatc cctcgttcgg aaagaaaagc gcgcacttcc gcttccgttg
     1081 cgtcctcaac ccatgcgcta cgatatggcg gcacggcaca ttcatcgccg ataaacaacg
     1141 cattgtaatc ggcagagact tgcgccatat cacagctttt ctgtaaacgc gtcagtaact
     1201 catcctgctc cagtggccag ttcgcagcca gtttcccctc acgaatcagg gtaaacagcg
     1261 gcaccagtaa aggatcttgc ggttggcggt aatagagcga acccagcaca cgacagagga
     1321 tagaaaactc gttcattaat atatttccat tactagtaat taaaggtctg caaattccgc
     1381 aatcggtgcc ataccgcgag attcaaggaa gttcagtaag cggcgcggag aaacattcaa
     1441 aatgcgctct ggcggaaaat ctaccgcgtc gaggatttta agacactctt caaattcccc
     1501 catggtaaac gcggtgtgag aatccgagcc taatgccacc caacctccag catcacgtac
     1561 cgctgcggct accgcacgac agttgtcttc actgccctta cgtgagtgta aaaatgaaga
     1621 attattgatt tccagcgcca cctgatgttt cgcggctgct tcagcaacgg ctttcacatc
     1681 tatttcatat ttgggatttc cgggatggct tattatatgc acattgccgc tggcgatagt
     1741 ggcgatcatc gcttgtgtat tggtcgcttt gtcatgtggt gcaaaaaccg gctcatgaaa
     1801 accggcaata atcagatcca gcgagtcaaa cattttaccg ctgcaatcaa tttcaccatc
     1861 aacattttta atgttagctt cgatgccgcg caggatccct accccatcaa ccactcgcgg
     1921 ccagatacgc atgttaatga agtgccagtg atgcggagca tcttccatat ccgggccatg
     1981 atcggtgatc gcaaaaagtt taatgccctt ttgtttggcc tgggcaatgt aatcactcaa
     2041 tgtgctatat gcatgtgtgc tggcaacggt atgcatatga aggtcgacgg gatacataac
     2101 tctctcctgt attcaattcc cgccaaggat agcaggaatc ctgatgcttt attagtagcc
     2161 gcgtgcgcgg tcgacttgcc cgcagaccct ctcccctttt tcgagctggg caatggtgcg
     2221 agaaatgtac tccacagctt cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt
     2281 cacgcgtgga tgttgccaga gcggactttc aggcggtaag ggttcacgat taaaaacatc
     2341 caacattgcg cctttaactt tgccgctatc cagcgccgcg agcaggtcat cttccacaac
     2401 atgaacacca cgcgccaggt tgaggagata cgcgccatcc ggtaattttt cgagtaattg
     2461 ttgattaata atgccgacgg tttcaggggt attcggtaac aaattaatca atacccgaca
     2521 ttggctcaga aatgcagaca gttcttcccg tccggcaaag ctttgcacgc caggccacga
     2581 tttacgggtt cgactccagc aacgcagcgg aaagcgccag gtttgcagac tctgagcaac
     2641 tttactgccc agtacgcctg cgcccaaaat gccgatggta aaatcttccc aatgatattc
     2701 aggcagcggt tgccaatgcg aactattttg ctggatgcga taatcgtcaa aacgtcgaaa
     2761 ccaatgcagc acctgactga cagcatattc ctgcatttgc tcgcccatac cagtatcttc
     2821 caggcgaaaa agtggaacag aagggttcag catttcaggg tgtgcctgta gcttgctcaa
     2881 aatagaatca acaccggccc cgagtgcgaa caccgcttta agatcgcgcc ctgccagcat
     2941 ttcaacagga ggatgccaga ctaaagcata atcagcagag tcattatctc cgcttttcca
     3001 tgctctgact cttgcctgag gaatagcttt gcgcagtgcc tcaatccacc attgggtatc
     3061 gaacgttggg tgataaaaga tgatatccat actgactccc gaaaagcgtt gtgcgaaatt
     3121 tattcgcact tatcgttatg atctacaaag gccaccagca taacaaatcc gtggtcggtg
     3181 gcaaaaaaag cagatttcgc ttattaaaac cacacattga ttgaagtttg aataaacgcg
     3241 cgattttttc aaaaagtttg ttgacctcag gtcatgattt ccctaaatta gcgcccgttc
     3301 cagcaagaca ggaacaacaa tttggtgagg tgtccgagcg gttgaaggag cacgcctgga
     3361 aagtgtgtat acggcaacgt atcgggggtt cgaatccccc cctcaccgcc agattataag
     3421 cgcatgttat tgtttaacat gcgctttttt atttttcctt ccagacagta tgccatcggg
     3481 tatgccatct gaaaccggtt tatatgcccc gcgatcatat cttatcggac aatttccgat
     3541 ctttgagcat actggcaaag ctatactgcc agcatatcag gatagtgttt tgcgataata
     3601 tcattcagtc tgtcaaccag ttctggtttt ctgaacgtga tatgcgcgga gcccttctta
     3661 aagtatctga tgctgaacat ctcatcttca tagctctctt taccctgaac agactgaatg
     3721 tgatcatcca gacgaagggt gatgtttccc cggttgtcgg ggattgtttt accgctgaac
     3781 aggtgcaaca ttctttccag atcggcgagt cggtcagcct gttgtccggt gataagatga
     3841 aatccccacc ggtcccatct caccagattg ttgacgataa ttttactgcc aaatttacag
     3901 gggcaattgg ttttgtaatt ccagttcagc cctctgaaga cgttgatcac ccctcgctca
     3961 aacacctcat ccttattctg gtgcagttgt tcaaatgtgc tcagaatgtt cgcttcgctg
     4021 atttccggaa aattatcgta ctccagtgac cggtaccatg tatcacgggc ctgcgtatcc
     4081 attaatgaca gcatcccgga acgttgcatc aggtcacgcc agatttcgcg atcaagattc
     4141 cgggtaatgg ctttcattgc tgtttcgggt ttctccatca gccagcagcc gcagcgaaaa
     4201 tcctgtttca tggcccagtc cggggcagtt ttacctccga tgctttccgt cagcaccgag
     4261 atttcagcca gttgtcggat aagtgtgtcg atttgcaaca gtgtactctt tcgtacagcc
     4321 agaatgtggc gaatgctggt tgagcagata acgtccgtat gcccgttgac cacttccgga
     4381 cgggagattg tgatatctga cataatcgat attccttttt gcagataaat gaaaacgcca
     4441 gccctgtgca ggctggcgtc agaggaatga agtctggtga gtgagcttca ccggttctgg
     4501 tgcagaagct gcagatgacg cagggtacgg acatcctccc gggcctgttc gatacagggc
     4561 aacagtgctg ctgaatccgt tttatcctca tcgttgtcaa agataattcc cgatccgcag
     4621 tcgatattgt cctgcagcca cgcaatcaga atatccagcg ctgtttccgt ggttaatgat
     4681 ttcatgttgt tcctggttac cagtcgaaag tgggtaaacc cggcaggcat tcagcgctgg
     4741 catccagatg aatgagactg acgccataac gccggatgag tgtgacgacc agacgacgga
     4801 acgtttttga cagccccagg cgtttaagcc gcagaaccgg gtatgaccac gcatcggtac
     4861 gtaacagata tccgctacct gtaaaatgaa tccattctga ttcaccgaag tcactggtct
     4921 ggtgtgacag cgagtacaac caggcgttgt ccgtttccgt gatatgtgcg gtactgcagc
     4981 gtatgccggt aagggtagca aatggtggcg tgcattctgc tgaggtctgg tcagattcat
     5041 caacaacacg aagggagtag ccgttatcgc agaccatgtt aatcagttca gcgagctcaa
     5101 taccatcgac gtcaacgaca atgcgcccca tgttcagtgc ctgtacgtta acgctgtcgg
     5161 cttccagtgt cagggccagt ttcatcattt cgcctccgga tacttaccca gggtaatgtt
     5221 atttaccgtt ctgtaattgt cgcgggtcat caggccggtc gcccggcgag cccggaggat
     5281 atcaatactg tttattaact gagagcgggg acaggtgctg aatcccggct ggtcggtacg
     5341 caccagcgcg tatttttcca cgagaaaatt aactgcatca cacagtgaaa tgcctgcctc
     5401 aatatgctgc tcaatcacac gttcatcgac aaacggtgtg tcattcagtg tgaggccata
     5461 atgctggtcc agcagtcgga tgagcagtgt ctgccagatg gtgacgggag acgggcagcg
     5521 agatacctcc cgtacatgtg tgtcggataa tgttttcatc ttaagttctc tgtaactggt
     5581 agtgctaaag gtgagagctt cggggttatg aggtggttgc gggtgctggt gtcggataaa
     5641 cagccagata aacgtaacca caggagccga gggtgtcggc ttcgcaggtc agcccttttg
     5701 catacagcgt gacggtatgc tgatatcggg gattcagttc accgctggtg agcatgagtt
     5761 ccagttgttt catcagcagc ggaaaggcct ggtccagatg gtatgcatct gcgtcgctga
     5821 agcggcctct gataccggcg cggtcggcaa ggtaatacag gcggttaccc tcttgcacca
     5881 gacgagcgcc aaaacagggc ctcactgtgc agggaagccc ccaccagggg cggtcgtgat
     5941 tgtcatcggg atgagttatc ccggagagtt tgtctgacac gataaaatcc tctctgaaaa
     6001 gtggttaaga atggtttaac gatgacaggt cagccggcgc gctgaccggt ttcggtgaag
     6061 atgtatgcat tgatgagaat ggcctcatcc acagcggcgt ctgaggtcat ctggtcgtac
     6121 tcgtttcccg ggtgacggaa catcagttca gggtgacact catcagccgg acatacgggc
     6181 caaaactgtc cttacggcgt tctgcaaaca cggcaagaac gccgggaata tcctgcactt
     6241 cacgaccagt atacgcttca gcactgccgt gccagcgata cttaccggta cagaacggaa
     6301 aaagacggga tgcaggatgc tgttggtgaa tacgcatggc ttcaccacgg gtgatgattc
     6361 tcatgatgtg atacctctga agacagaaga taaaagtgaa aacaggtgtg atgtggttgt
     6421 gacgggttaa agcagaccat gttcggcaaa ggagaaaacc tggttgccac cgactatcag
     6481 atggtccggc acccggatat ccaccaggcc cagtgcctgt accagacgtt cggtgataag
     6541 ccggtctgcc ttactgggtg tgacttcacc ggacgggtga ttgtgcgcca gcaccacggc
     6601 agcggcattg tggtacaggg cgcgtttaat cacttcccgg ggatgaactt ccgtacggtt
     6661 gatggtgccg gtgaagaggg tttcaccggc aatcagctga ttctggttgt tcagatacag
     6721 cacccggaac tcttcccgct ccagtcccgc catgttcaga atcagccatt cccgtgccgc
     6781 acgggtggag gtgaaggcca cgccgggttc atgaagatgg cggtccaggg tttgcagggc
     6841 ccgcagaatg agactgcgct cgccgggcgt catgtttccg ggcagaaagg aaagctgctg
     6901 cattgtccct gcctccattc agtcgatgat gcgcataatg gcgctgcatt ccggatgctg
     6961 cagggcgtaa tcccgcagcc ggtaataatg gaccgtcatg gcataacact ccgtacgaca
     7021 ggcatgatga ctgtacgtca tcagacaggc agcaatgccg gcggcttccg ggctcatttc
     7081 agcgcggtta ccgttcatgg cattgaacag tacccatgtt tcgtcatcat cgtcatccgg
     7141 ttcgggtgcc ataaatgccc cgccgttgtt cagggtgtac agattccaga taccaccgca
     7201 gtagtcttca cacagacggt ccatccagcc gaagacacgg ggttccaggg tgacccactg
     7261 tggaatgagg ccaaaatgct gcggccagaa gctgatgcgc tgttcatcag ggacgggagt
     7321 ggcaaccagt tgttgcaggc cagtctccgg tgcacaggcc gaagcggtgg tgttctgaga
     7381 caatgttttc atgtatgaaa tccttgtaat gtgaataaat gagagggagt aaaggtcggg
     7441 agctggcaac atcacgttca tcactgaccg tgatgacccg gtaatatcag ggatgaatga
     7501 tattcggctg aaattccgga cagccgtacg cgtaaccgtt ccggtcacag acggatggcc
     7561 agatgccatt gcctgtatac tcagtctgca cagactgatt tctccgggac ggtaaagcag
     7621 acgggtacac caggcgcaca ggtcatcatc agaaatggaa cgacaccccg gcagcgcctc
     7681 cggggcgtcg ggtgattctg gtgcctgaat cacgccttca gggacatgct gcatcagcgc
     7741 aggctctcca gcagcgtttc tgccattacc cacaatgcgc ggttgagctt aatatcggtg
     7801 tcgatgttgt gaatggcacg ggtgtggata cgttttcctt ttgcactgcg gccggaaatc
     7861 ccgcctttca gcatattctc ctggatggtc tgatatgcgc tccacaggtc tttaccgtaa
     7921 tcctccctgc gtcgtggtgt cagaatgtca gcggtggtga ctggctgatg ttcgtcacca
     7981 taacggtaag tcagtgccgc ctgtgccagc gcctggcgtg ccggtggcgg caggaccagc
     8041 gactgcatgg catcacgctt ttcctcaatc cggtcaaaaa cccccaccac ttcgtaagcc
     8101 ccttcgataa ctttctccac cacatttccc cggtgtggaa cacgcacttc ccccagagac
     8161 tgaccgcaga cgcagccatt ctggcagacg aatctgaagt aacccggcag catctggtag
     8221 ctggaggtac cgtcatgaga gttgagcaga ataatttcag ggacatgttc tccgtttatc
     8281 tctccggccc gccgcagacg cagcatgtgt tttgtgtatc cccggcggcc cgggtcgcgc
     8341 acgcgggtct ggcaggcgaa gaatggctga aaaccttcct gctgcaggct ttccagtacg
     8401 gtgatggtgg gaatgtacgc atatcgttta ctgcgggaag tatgccggtc ttcaccgaaa
     8461 atactgggaa catagtgcat cagttcttcg tgtgtcagcg gacggtcacg gcgtatctgg
     8521 ttcgcataac caaaacgact ggctagtcgc ataatttgct ccttatcggt ggttacgatt
     8581 tactggtgta ataaatgaaa aagctgcgtc tcccggagaa gacgcggcct gacagatgaa
     8641 atgaatgacg tttattgtct gagaagccct taactggcga gctgagtatt aagctgtgtt
     8701 ccggcatcac cagcgcaact gaccttcagc attacggata accagccggg aatatgttcc
     8761 ctggtcatct tcagtaaaca cattgcggta agctgttttg acggcaacag cctgttcgcg
     8821 tgagaaaggg ccttcgggca ataaacgtga tgtacaaccc ggcatatctg ttgctccctg
     8881 aaagtaaaaa gccccggtca tgatgaccgg ggcctgaagg agagtgacct gattatcaaa
     8941 aagtcacatt cagcgtggcc tgaccgttat aaccttcagc gctgctgccg ctgacgctgt
     9001 gggcataacc ggcctgaacg cccagggtga tattttcccg gacacgggct tccagtccgg
     9061 cctgcaggtc cagtgacgtg ccattccggg acggtgagaa cgtcatgtta ctgccggcgg
     9121 cggctgtccc catgctcatg tcaccccggg agctgaaagt gcggataaca gaaggctgta
     9181 cccaccagtt caccggcagt tcactcacac ggtgttttgt actgtcgcgc agagtgtcac
     9241 gggatgaggt gccttcacca aagctcatat cgttgtggct gcccagacgg aaaccggcac
     9301 gcacatgttg tgcactgcca tgcccgaact tcacataacc ggcgttatcc tggccgtcat
     9361 ccagggtaag tccctgccag gtatagtgca gttgtggctc cagcatcagg ttgtcagtga
     9421 tactgaaggg cagaccggtt tccagtgagc ccagccagcc ccagccccgg gcacggaagt
     9481 cgttattgtc cgatgacgct ttcatgctgt ggcgggttcc ctgtgccaca atgtcagccc
     9541 acaggccgga ggacgtgtgt gtcagattca ggtatccgcc caggctgccg gcatcatccc
     9601 ggaccgtgcc ggcgcgggaa ccgtcatcat ccttaacatc aacggaagaa tggcctgcgg
     9661 caccatacac ccctgtcgtc agagacatac cggcaacctc tgttctcagc aggtcaccct
     9721 ccagacggac gaagccatag ctgccgctgc tttccggcgt ggctccacgg gcaataccgc
     9781 cgttgttatc gtgaccgaga tgaccgccct gaatgctgag acggacgctg ttattttcac
     9841 cgtttacacc ggtctgatgg ctgcgggagc ctgccagaat ccggtcatag tccattgcct
     9901 gtgtcagcat ggatgtatac agggggactt cagcacgata agcattttca ctgcgcaggt
     9961 accagtcttc atcgctgtca cggttcaggg tgtagttaaa ggcgccggcc tgaagcgggc
    10021 gactcagggc aaacgcacct tcttctgtgg tggcgccatt ctgtgcatcc acaacccgga
    10081 taccctgtcc ggaggttgcc accccgaggt tactgtttcc gacatttgta aacgcaagcc
    10141 aggttttgcc ggttgcctga ccaccattaa tcaccagctg gtcagaggta ttgctgccat
    10201 caaggcgaac acgcatattg atggtgccac cctgaccggt gaggttactg gtggtcagtt
    10261 tatggaacgt taccggggag tcgccttttg caacagcacg gctcagcgcg gcatccggtg
    10321 tcgtctgatt atcaaagcga atctcccctg cgttaacaat atcgccggta ctgaccacat
    10381 cgccattcag taccatgctg gcacctttac tgagctgtgt tctgccactc agacttcccc
    10441 cggcagccag cgtcagtgtt ccacgatgtc cgacagtggt gttgctggcc agtcctccgg
    10501 cattaaccgt aaagctgccg ccattttcca gcagcagtcc gctggcctga ccggatgtgc
    10561 catcaatact gaacttaccg ctggcattgg tcccctcaac agtggcacca ctgtctgcaa
    10621 tcagggcacc accggatgtc atggtgacac ctgttgcctt accaccggca gacactgcca
    10681 gggttccgcc gtcatccacc cgtgttttct gcgctgaatg gccttccagt acatccaggc
    10741 gaccgccgga ctccagaaca actccgtcag ccttaccgtt ttccaccgtg aaattgccca
    10801 gacggttgct gccaagaacc gttgccgcag tactggtaac cagtgctcca ccctgtttca
    10861 gggtgacatg tgtggcggta ccaccggcgt tcacctgcag tttgcctttc tggttaacgg
    10921 tggtgaaatc cgccagacca ccttccttga taatctgcca gccgtcactg tttacaacag
    10981 tgccagacgc cgtaccgccg ttgtgcacat actggtaacc gccattcagt gtggtatcca
    11041 gcgcatgccc gtgtaccgtc tggtcgccgc cggcataaac cacagtggta tttgctgttc
    11101 cttcagcagc cacaatctga cgaccatttt cattgatagt ggtacgtacg gcatttccgc
    11161 gaacaaactg cccggtatca ccattttctg catctggtcc cccttccgcg ccggtattca
    11221 caaccgtgtc ggttgccatt gcgccggatt tgacggtctg ccagcccttc tcattaatga
    11281 cggtacccgt tgcaatcccg ccttcatgta cccactgctc accgccgttc agagtggtat
    11341 tcactgcctg cccctgaagg ctctgtccgc ctccggcact gataaccgtg tctgaaacgc
    11401 ttcctccggc attcactctc tgaagaccac caccggtgac agtagtgttg ttggcgatac
    11461 cgccattttg tatccattgc ccgccggtat tggcctcgtt atccggccca tactccagac
    11521 cgctactgat ggtcattccg ttggccgtac cgagaacaat ctggttgtca tgatttgtca
    11581 gtgttccgcc gttcacggtt tctcccgcct gtacaatcgt gtcagcagcc agtgccggga
    11641 ctgatgtgac agcagcaaga gacagcacaa ccgccacacc ggcgcgtttt ccccgtgagc
    11701 gggccagttc ggaagccacc accagggtgc ccgtaatgtg attccatacc agcctgtagc
    11761 tggtgttcag atgtcgtttc atcagctttt ccttacagag ggtgaataaa aaagccggta
    11821 cccacaaaag tggataccgg caggtacaaa acagcatggt cagataaagt ggtttttccc
    11881 ggacataaat gcttcccctc tctcatccgc cagagaagta tcagtccgtt gtgtgcaatt
    11941 ccgttctgca tgatgaagag attcagctgc cttttcgcag accattaacc actataaacg
    12001 atcgatagca acgattgata cctgttttat cgatcgtttt attctattaa cgacaaggcg
    12061 gtcacgcaag agaaacagga ggagtatgag gggtttgtgt catgttttca gttggttaac
    12121 gtaatgttat caggcatttc cctggcagac tgttcagaag aaaatccagt tccatacagc
    12181 gcgggcaaca gagaccaccg tgtcacggac ggcacgcaga accgtacggg caacagaggc
    12241 aacacagacg ctctccgccg tgtcaaatat ccggtccacc gcaccggtaa actgttcacg
    12301 ggcctgaccc cggacagatt ccgtgcgcag ctcgtcctgc aggcgggtca tcaggggact
    12361 ggcggcatgg tcgggaagtg ctgtcattag cgcactgacc agcgtatcca gttcccagcc
    12421 ggtgcgggcc gatacggcaa caaccggatg tacgggccgg aacagacgga ataccgcctc
    12481 cgttttttcg cgaatattct gtgcctgtgc gggagaaggc tgaataccgg ccatatccca
    12541 ttcatggcag ggctccgttt tgtcggcctg cgtcaccaca aacagcacct gctgatgtcc
    12601 ccggtgcagg atgtgtcgcc agaaatactc atccacagac agggcacggt catcggcttt
    12661 aatcagccac a
//
LOCUS       DAJTYD010000107        12636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTYD010000107 DAJTYD010000000
VERSION     DAJTYD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.107"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..307
                     /locus_tag="OO585_002720"
     CDS             <1..307
                     /locus_tag="OO585_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6630255.1"
                     /translation="TQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQ
                     QLPPGISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(316..714)
                     /gene="vapC"
                     /locus_tag="OO585_002721"
     CDS             complement(316..714)
                     /gene="vapC"
                     /locus_tag="OO585_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6630256.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(714..941)
                     /gene="vapB"
                     /locus_tag="OO585_002722"
     CDS             complement(714..941)
                     /gene="vapB"
                     /locus_tag="OO585_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6630257.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1023..1442
                     /locus_tag="OO585_002723"
     CDS             1023..1442
                     /locus_tag="OO585_002723"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6630258.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1429..6300
                     /gene="traI"
                     /locus_tag="OO585_002724"
     CDS             1429..6300
                     /gene="traI"
                     /locus_tag="OO585_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR6630259.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQYVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVMAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6320..7066
                     /gene="traX"
                     /locus_tag="OO585_002725"
     CDS             6320..7066
                     /gene="traX"
                     /locus_tag="OO585_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6630260.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7121..7681
                     /locus_tag="OO585_002726"
     CDS             7121..7681
                     /locus_tag="OO585_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6630261.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7812..8024
                     /locus_tag="OO585_002727"
     CDS             7812..8024
                     /locus_tag="OO585_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6630262.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8268..8729
                     /locus_tag="OO585_002728"
     CDS             8268..8729
                     /locus_tag="OO585_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6630263.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8775..8984
                     /gene="hha"
                     /locus_tag="OO585_002729"
     CDS             8775..8984
                     /gene="hha"
                     /locus_tag="OO585_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6630264.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9023..9613
                     /locus_tag="OO585_002730"
     CDS             9023..9613
                     /locus_tag="OO585_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6630265.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            9853..10113
                     /gene="repA"
                     /locus_tag="OO585_002731"
     CDS             9853..10113
                     /gene="repA"
                     /locus_tag="OO585_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6630266.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10337..10411
                     /gene="tap"
                     /locus_tag="OO585_002732"
     CDS             10337..10411
                     /gene="tap"
                     /locus_tag="OO585_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6630267.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10404..11261
                     /gene="repA"
                     /locus_tag="OO585_002733"
     CDS             10404..11261
                     /gene="repA"
                     /locus_tag="OO585_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6630268.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12341..>12636)
                     /locus_tag="OO585_002734"
     CDS             complement(12341..>12636)
                     /locus_tag="OO585_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6630269.1"
                     /translation="YRLVFQFPIREQAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEI
                     RAKLYLASLCGLRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3066 a         3190 c         3742 g         2638 t
ORIGIN      
        1 gactcaggct gaacagccgc aacagccggt gtctcctgtc atcaacgata agaagtcaga
       61 ttcaggtgtg aatgttccgg caggggggat cgagcaggag ctgaaaatga aaccggaaga
      121 agagatggaa cagcaactgc cacccgggat cagtgaatcc ggtgaagtgg tggatatggc
      181 cgcttatgag gcatggcagc aggaaaatca tccggacacc tggcagcaga tgcagcgtcg
      241 tgaagaggtg aacatcaatg tgcaccggga acgcggggag gatgttgagc cgggagatga
      301 tttctgatga acaggtcagc tccagtcttc agttctcagg ccgcccacac gttcaaattc
      361 ccgggtgtta ttagtcacaa taatcagccc ccgactgcgg gcatgacctg cgatcatttg
      421 atcaaatggc ccgacagggc gtccctgacg ggcaagttct gctcttatct ggccggtgtg
      481 tgtggctgca gcagcgtcgt aatccagaac atcaatgcgg gaaacaaatc cctcgatcac
      541 agcgagatta cgttcaggca tctggctttt ttctgcacca tatatcagct ccatcagggt
      601 gaccgaactg atacacattc tcccctggtt caggttaaaa cgctccctga cgctggcggg
      661 tttgttcttt atcgtaaaaa tgcagatgtt ggtatcgagc ataaacttca gcatcagaat
      721 gactcccttt cctgcatgcc gggctgttcc ctgttatcca taaaatcggc gctgacgctg
      781 tgtccgtcga accattcgtc ccatgtctct ccggctggcg taatgattct ggttcgtccg
      841 acagcaatca cttcaacgcg ttttacgttt tccggcaatg caaccgcttt tggcagtctg
      901 accgcttggc tgcggttgct gagaaatacg gtggtttcca ttatttgcct ccttatgtgg
      961 atatgtcttg tgtatatcca ttgtgggatt gataccggga tatgtcaaag ggatatacgt
     1021 ttatgatgag tatcgcgcag gtcagatcgg ccggaagtgc cggtaattat tataccaaca
     1081 aggataatta ctatgtgctg ggcagcatgg gagaacgctg ggccggccag ggggctgaac
     1141 aactggggct gcagggcagt gtcgataagg atgtctttac ccgccttctg gagggcaggc
     1201 tgccggacgg ggcggattta agccgcatgc aggatggcag taacaggcat cgtcccggct
     1261 acgacctgac cttctccgct cccaaaagtg tctccatgat ggccatgctc gggggggaca
     1321 aacgtcttat tgaagcacat aaccaggccg tggattttgc tgtccgtcat cacccgtgtg
     1381 gaggcactgg cctccacacg gatgatgacg gacggacagt cagaaacggt gctgaccggt
     1441 aatctggtga tggcactgtt taaccacgac accagtcgcg atcaggaacc acagttacac
     1501 acgcatgcgg tggtgactaa tgttacgcag cataacggcg aatggaagac actgagcagt
     1561 gacaaagtgg ggaaaacggg gttcattgag aatgtgtacg ctaatcagat tgcctttggc
     1621 aggctctacc gggaaaaact gaaagagcag gttgaggcgc tgggctatga aactgaagtg
     1681 gtcggtaagc acggtatgtg ggaaatgccg ggtgtaccgg tggaggcctt ttccggacgc
     1741 tcacagacta tccgggaggc cgtcggggaa gacgcctcgc tgaaatcccg ggatgtggcg
     1801 gcgctggata cgcgtaaatc caaacagcac gtcgatcctg aagtcagaat ggccgagtgg
     1861 atgcagacgc tgaaggaaac cgggttcgac atccgggcat atcgtgacgc agcggaacaa
     1921 cgggcatata cccgcacgca gacacccgga cccgcttcac aggacgggcc ggatgtgcag
     1981 caggcggtga cacaggccat tgccggatta agtgaacgca aagtgcagtt tatgtatacg
     2041 gacctgctgg ccaggacggt cggcatactg ccgccggaaa atggtgtgat tgaacgggca
     2101 cgcgccggta tcgatgaggc catcagccgt gagcagctta tccccctcga tcgtgagaag
     2161 gggctgttca cgtccgggat tcatatgctc gatgagctgt cagtccgggc actcagtcgt
     2221 gacatcatga aacagaaccg ggtgaccgta catccggaga aaagtgtccc ccggacggcc
     2281 ggttacagct atgccgtcag tgttctggca caggaccgtc cgtcgctggc cattgtgtcc
     2341 gggcagggcg gtgcagccgg gcagcgtgag cgggtggctg aactggtcat gatggcccgg
     2401 gagcaggggc gggaggtgca gattatcgct gctgaccgtc gctcgcagat gaacctgaag
     2461 caggatgaac ggctgtccgg tgaactgata accggacgtc gtcagttgct ggaaggcatg
     2521 gccttcacgc cgggcagtac cgttatcgtc gaccagggcg aaaaactctc cctgaaagag
     2581 acgttaaccc tgctggatgg tgccgcacgt cataacgtac aggtcctgat aactgacagc
     2641 gggcagcgaa ccggtacagg cagtgcactg atggccatga aggatgccgg ggtgaacaca
     2701 tatcgctggc aggggggaga acagcgaccg gccaccatca tcagtgaacc ggaccgttat
     2761 gtccgctatg cccggctggc cggagatttt gcggccagcg tgaaagccgg agaagagagc
     2821 gtggcacagg tcagcggagt acgggaacag gccatactga cacaggccat tcgcagtgag
     2881 ctgaaaacac agggcgtgct cggacacccg gaggtaacca tgaccgccct gtcaccggtc
     2941 tggctggaca gccggagccg ttatctgcgg gatatgtacc gcccgggaat ggtgatggag
     3001 cagtggaacc cggagacacg cagtcatgac cgctatgtga cagaacgtgt gacggcgcag
     3061 agtcacagcc tgaccctgcg gaatgcgcag ggcgaaacgc aggtggtgcg tatttcttcc
     3121 ctggacagca gctggtcgct gttccggccg gaaaaaatgc cggtggcaga cggtgagcgt
     3181 ctgagggtga cagggaaaat tcccggactc cgcgtctccg gcggtgaccg cctgcaggtg
     3241 gcatccgtca gtgaagatgc gatgacggtt gttgtgccgg gacgggctga accggccacc
     3301 ctgcctgtga gcgattcacc gttcacggca ctgaagctgg agaacggctg ggtggaaacg
     3361 cccgggcatt ccgtcagcga cagtgcgacg gtttttgcct ccgtcacaca gatggcaatg
     3421 gacaatgcca ccctgaacgg tctggcccgc agtggtcgtg atgtccggct gtattcctca
     3481 ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc cttcctttac ggtggtttct
     3541 gagcagataa aggcgcgtgc cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa
     3601 gccgggcttc acacgccggc acagcaggcc attcatctgg cccttcctgt gctggaaagt
     3661 aaaaacctgg ccttcagcat ggtggacctg ctgacagagg cgaagtcgtt tgctgcagaa
     3721 ggaaccggtt ttgctgacct gggaggggaa atcaatgcgc agataaaacg gggtgattta
     3781 ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc tggtttcccg tgcgtcgtat
     3841 gaggcagaaa agagcattct tcgccatatt ctcgaaggta aggaggcggt cacgccgctg
     3901 atggagagag tacctggcga actcatggag aaactgacat caggacagcg cgccgccgcc
     3961 cgtatgatac tggaaacgtc cgaccgtttc acggtggtgc agggctatgc cggtgtgggt
     4021 aagaccacac agttccgggc ggtgatgtca gccgtgaaca tgctgccgga gagtgaacgt
     4081 ccccgtgtcg tggggctggg gcccacacac cgtgcggtcg gggagatgcg cagcgccggc
     4141 gtggatgcac agacactggc gtcctttctg catgacacgc agctgcagca gcgcagcgga
     4201 gaaacgccgg atttcagcaa cacgctgttc cttctcgatg agagctctat ggtgggcaat
     4261 accgacatgg cacgggcata cgccctgatt gcggccggtg gcggtcgggc tgtggccagc
     4321 ggtgacacgg accagctgca ggccatcgcg cccggtcagc ctttccgtct ccagcagacg
     4381 cgcagtgctg ccgatgtggt catcatgaag gagattgtgc gtcagacgcc ggaactgcgg
     4441 gaggcggtat acagcctgat taaccgggat gtggaaaggg cgctgtccgg gcttgagcgt
     4501 gtgaaaccgt ctcaggtgcc acgtctggag ggcgcatggg caccggagca ctccgtgacg
     4561 gagttcagtc acagccagga agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa
     4621 ggcgaggctt ttccggatgt ccccatgaca ctgtatgcag ccattgtccg cgactatacc
     4681 ggcaggacac cggaagcacg ggagcagacg ctgattgtca cgcacctgaa tgaggaccgg
     4741 cgcgtactga acagcatgat tcatgatgca cgggaaaagg ccggagaact ggggcaggtg
     4801 caggtcatgg tgcctgtcct gaacacagcg aatatacgcg acggggagct gcgtcgtctc
     4861 tccacctggg agaataaccc ggatgccctt gccctggtgg ataatgtgta tcaccggatt
     4921 gccggtatca gcaaggatga cgggctgata accctgcagg atgcggaagg taacacgcgg
     4981 ctgatttcgc cccgggaggc ggtggctgaa ggcgtcacgc tgtacacccc ggacaccatc
     5041 cgggtgggga ccggtgacag gatacgcttc acgaagagtg accgggagcg cggttatgtg
     5101 gccaacagcg tctggacggt gacagcggtt tccggtgaca gtgtcacact ttctgacggt
     5161 cagcagaccc gtgtgattcg ccccggtcag gagcgggcag agcaacatat tgacctggcc
     5221 tatgccatca ccgcccacgg tgcacagggg gcgagtgaaa cctttgccat cgcgcttgaa
     5281 ggcacggaag gtaaccggaa actgatggcc ggctttgagt cagcctacgt ggccctgtcg
     5341 cgtatgaagc agtatgtgca ggtgtacacc gataaccgtc agggctggac ggatgccatc
     5401 aacaatgccg tacagaaagg aactgcccac gatgtgtttg agccgaaacc ggaccgggag
     5461 gtcatgaatg cagagcggct gttcagtacg gcgcgggagc tgcgggacgt ggcggcaggc
     5521 cgtgccgttc tccgtcaggc ggggctggcc gggggagaca gtcctgcacg gtttattgct
     5581 ccgggacgta aatatccgca accgtatgtg gcactgccgg cgtttgaccg taacggcaag
     5641 tccgccggta tctggctgaa cccgctgacc acggatgacg gaaacgggct gcggggattc
     5701 agcggtgaag gtcgggtaaa aggcagcggg gatgcgcagt tcgtggccct gcagggcagc
     5761 cgtaacggag aaagcctgct ggctgataat atgcaggatg gcgtccggat tgcccgtgat
     5821 aatcctgaca gtggtgtggt ggtaagaatc gccggtgaag gccgtccgtg gaatcccggt
     5881 gccatcaccg gtggccgcgt gtggggggat atcccggaca acagcgtcca gccgggagcc
     5941 ggaaatggcg aaccggtcat ggcagaggta ctggcacagc ggcaggctga agaggccatc
     6001 cgccgtgaaa cggaacgtcg cgcagatgaa attgtccgta aaatggcaga gaacaaacct
     6061 gacctgccgg acggcaaaac agagcaggct gtcagggaaa ttgccgggca ggagcgtgac
     6121 cgggctgcca taactgaacg ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa
     6181 cgggtgcggg aagcggtccg ggaagttgcc cgggaaaatt tgctgcagga gcgtctgcag
     6241 cagatggagc gggatatggt tcgtgacctg cagaaagaga aaaccccggg cggagactga
     6301 tacaggaaga caaacactga tgacaacgga taacacgaac acgacacgta acgattcact
     6361 ggctgcacgg accgatacct ggttgcagtc atttctggtc tggtcacccg gacagcggga
     6421 tatcatcaaa acggtggcac tggtgctgat ggtactggac cacatcaatc tgatattcca
     6481 gctgaagcag gaatggatgt ttctggccgg gcgaggagcc tttccgcttt ttgccctggt
     6541 gtgggggctg aatctgtccc gtcatgcgca tatccggcaa ccggccatta accggctgtg
     6601 gggatgggga attattgccc agttcgcata ttatctggca ggctttccct ggtatgaggg
     6661 gaatatcctg tttgcctttg cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag
     6721 tgggtggcgt acagcagcgg ccatactcct gatggccctg tgggggcctt tgtccggcac
     6781 cagttacggt attgccgggt tgctgatgct ggcagtcagc aaccggctgt accgggcgga
     6841 agacagagcg gaacgtctgg cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa
     6901 ccttgccacc agtgatgcag cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg
     6961 tctggtgttg tgtgccggaa aatcattacc ccgtttctgg cccggagact ttttcccgac
     7021 gttctatgcc tgtcatctgg ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct
     7081 ttggccgcaa gaggatgatt cgtcagaggc agaacacagc atgacagagc agaagcgacc
     7141 ggtactgaca ctgaagcgga agacagaggg aacagcgact gtccgcagcc ggaaaaccat
     7201 catcaatgtc accacgccac caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc
     7261 ggcccgtgaa gcagagctgg cggcaaaaaa agcgcaggcc agacaggcgc tgtccattta
     7321 tctgaacctg cccacgctgg atgaggctgt gaataccctg aagccctggt ggccgggatt
     7381 atttgacggt gacacgcccc ggcttctggc ctgcggtatc cgggacgtgt tactggaaga
     7441 cgtggcgcag cggaatatcc cgctctcgca taaaaaactg cgcagggcgc tgaaggccat
     7501 cacccgttca gaaagctatc tgtgtgccat gaaagccggt gcctgccggt atgacacgga
     7561 agggtatgtg acggagcata tttctcagga ggaggaagcg tatgcgggag cgcgtctggc
     7621 gaaaattcgt cgccagaacc ggataaaggc agaacttcag gccgtgcttg atgagaaata
     7681 aaaaaagcct cccccagaga gaggaggcag ggaaataagg cttaaaagga aataacttcc
     7741 gcttccgaaa ggaagccagt ccactgaatt atttatcaca ataatactga caactaaagg
     7801 gggacaagaa aatgaatgga ttcagaaaca gttcacggaa cggccaggtc tggcgttacc
     7861 agcgtgccgg cgggcgggcc gttattctgg aagtcagtgg acgctggatg gaagcggcag
     7921 aagcatggcg acgggctgcc tgtgttgcgc ctaggacaga ctggcagcag tttgcccgga
     7981 aaagggctga acactgccac cggcgctgcc ggggaagggg gtaacgaaaa aagcagcctt
     8041 cattactcca ggagtcagaa ggctgaaagg agtttatgag atttgcttct taatatccgg
     8101 tcgggcagta tgaccggaca gttctgagta gcacagagtc ttttccgcat taagtcgatt
     8161 ttttatattt tgtgattaat ttcacaaaat aaggtgttat tcaatgtgtg ctgcactatt
     8221 caggggggct gaaatacagg tgccatttat ctgatatgga gaataacatg aggaaatata
     8281 ttccactggt attatttatc ttttcatggc cggtattgtc tgcagatatt catggacggg
     8341 ttgttcgtgt actggacggt gataccatcg aggtcatgga ctcactgaag gcggtgcgga
     8401 tcaggctggt aaatattgat gcaccggaaa agaaacagga ttatggccgg tggtcaacag
     8461 acatgatgaa atcactggtt gcaggaaaaa cggttaccgt cacatatttt cagcgagatc
     8521 gttatggtcg tattctcggg caggtttatg caccagacgg aatgaatatt aatcagttta
     8581 tggtccgggc cggggctgcc tgggtatatg agcagtacaa cacagatccc gtgctacctg
     8641 tccttcagaa cgaagccaga caacaaaaac gtgggctctg gtcagatgct gatccggttc
     8701 ccccctggat atggaggcac cgtaaataaa gcgatggcaa tcctatcatt catctgttct
     8761 cagcgggaat aaaaatggca aaaacaaaac aggaatggct gtatcagtta cggcgatgtt
     8821 catcagtcaa tacgctggaa aaaattattc ataaaaacag agacagttta tcaaacagtg
     8881 aacgtgaatc atttaattca gcagcagacc accggctagc ggaattaatc accggaaagt
     8941 tatatgacag gataccaaaa gagatatgga aatatgtaag gtaataattc agcaataatt
     9001 aattgccagg agcagataaa taatgaagtt gattattttt attctcattg ttctgattat
     9061 tgcagcgcta cttatccgga ttatcctcag aagtgtaaat cagcattctc ctttgctgat
     9121 gcaattgcat gcggcaggta tacggaccgg tgatgcagag cgaatactgt ccagcggtga
     9181 atactggcaa cgtcagacga cgctgctgac agaaagggaa gtcagtttta tgaaaggact
     9241 gttcagaatt gtggatatga cgcggtggta tctgtgtccg caggtacggg tcgcggatat
     9301 cgtccagctg aacgggaata tccggccacg atcacgccag tggtggcagt tattcaggat
     9361 ggtgtctcag tggcatgttg atgtggttat cgttgagctg cgttcgttca gtattgttgc
     9421 agcagtagag ctggatgatg ccagccattt acgaccggaa cgcagacgcc gggatattct
     9481 tctggaagag gttctgaggc aggctggtat tccgttgctc agaagccacg atgccagaaa
     9541 actgctgcag atgaccggag aatggctgaa tacaacaggg gctgatcagc agtcaccgga
     9601 acatcgtagc tgacgccttc gcgttgctca gttgtccaac cccggaaacg ggaaaaagca
     9661 agttttcctc gctcccggcg tttccataac tgaaaaccat actatttcac agtttaaatc
     9721 acattaaacg acagtaatcc ccgttgattt gtgcgccaac acagatcttc gccacaattc
     9781 tcaagtcgct gatttcaaaa aactgtagta tcctctgcga aacgatccct gtttgagtat
     9841 tgaggaggcg agatgtcgca gacagaaaat gcagtgactt cctcattgag tcaaaagcgg
     9901 tttgtgcgca gaggtaagcc tatgactgac tctgagaaac aaatggccgc tgttgcaaga
     9961 aaacgtctta cacacaaaga gataaaagtt tttgtcaaaa atcctctgaa agatctcatg
    10021 gttgagtact gcgagagaga ggggataaca caggctcagt tcgttgagaa aatcatcaaa
    10081 gatgaactgc agagactgga tatactaaag taaagacttt actttgtggc gtagcatgct
    10141 agattactga tcgtttaagg aattttatgg ctggccacgc cataaggtgg cagggaactg
    10201 gttctgatgt agatgtacag gagccagaaa agcaaaaacc ccgataatct tctttaactt
    10261 tggcgagtca gaaagattac cggggcccac ttaaaccgta tagccaacaa tcaagctatg
    10321 cggggagtat agttatatgc ccggaaaagt tcaagacttc tttctgtgct cgctccttct
    10381 gcgcattgta agtgcaggat ggtgtgactg atcttcaaca aacttattac cgccaggtaa
    10441 agaacccgaa tccggtgttc acaccccgtg aaggtgcccg gacgcttccg ttctgcggga
    10501 aactgatgga aaaggcggtg ggcttcacct cccgttttga tttcgccatt catgtggcac
    10561 atgcccgttc gcttggtctg cgtcggcgca tgccaccggt actacgtcgc cgggccattg
    10621 atgcgctgct gcaggggctg tgttttcact atgacccact ggccaaccgc gtccagtgtt
    10681 ccatcaccac gctggccatt gagtgcggac tggcgacgga gtctgctgcc ggaaaactct
    10741 ccatcacccg ggccacccgg gccctgacgt tccttgcaga gctgggactg attacctacc
    10801 agacggaata tgacccgctt atcgggtgct acattccgac cgatatcacg ttcacatctg
    10861 cactgtttgc tgccctcgat gtatcagagg aggcagtggc cgccgcgcgc cgcagtcgtg
    10921 tggaatggga aaacagacag cgcaaaaagc aggggctgga taccctgggt atggatgaac
    10981 tgatggcgaa agcctggcgt tttgttcgtg agcgtttccg cagttaccag acagagctta
    11041 agtcccgtgg aatgaagcgt gcccgtgcgc gtcgtgatgc ggacaggcag cgtcaggata
    11101 ttgtcaccct ggtgaaacgg caactgacgc gtgaaatctc ggaagggcgc ttcactgcca
    11161 gtcgtgaggc ggtaaaacgt gaagtggagc gtcgtgtgaa ggagcgcatg attctgtcac
    11221 gtaaccgtaa ttacagccgg ctggccacag cttccccctg aaagtgacct cctcagaata
    11281 atccggcacg cgccggaggc attcgcacgc ctgaagcccg ccagtgcaca aaaaatcagc
    11341 gctacataca aaaaacaacc tcatcatcca ccttctggtg catccggtcc cccctgtttt
    11401 tgatacaaaa cacgcctcac agacggggaa ttttgcttat ccacattaaa ctgcaaggga
    11461 cttcaccata aagttacaac cgttcacgtc ataaagcgcc agccgtcagc cctacagggt
    11521 gcaatgtatc ttttaaacac ctgtttatat ctcctttaaa ctacttaatt acattcattt
    11581 aaaaagaaaa cctgttcact gcctgtcctg tggacagaca gatatgcacc tcccaccaca
    11641 agcggcgggc cccgaccgaa gccggtttag ttacaacatg caaacataaa cccctaaaaa
    11701 acccaagtcc gacgcagaac tgaaaccaca aagcccctcc ctcataactg aaaagcggcc
    11761 ccgccccggc ccgaagggcc ggaacagagt cgcttttaat tatgaatgtt gtaactatat
    11821 tatcatcgct gtcagtcctc tggctggacg tatcgagtac acgctcgtaa gcggccctca
    11881 cggccctcac ggccctcacg gccctcacgg ccctcacggc ccgctaacgc ggagatacgc
    11941 cccgactgcg ggtaaaccct tgtcgggctc cgaccgcgca cagaagctct gtcatggctg
    12001 aaaacgggta tggcttggca gctaccatgt tctgtcaaga aaaatcgctt gaactccgac
    12061 gtacagcccc ctaagtactg ccagcaagct gtcagcacta agtccccgat acagcgcggc
    12121 gtagccgcgc taacggggtt aaaagggctt acctatactt catcatcgct gaaagcccgc
    12181 atggcctcgc agggcagaga tgggataggt gaaacctatc aatacgtacc ggcttacgcc
    12241 gggcttcggc ggtgttactc ctgtaccata tgtaactaca tcatgccgcc ttacatgcgc
    12301 tacgcgcata tgaagacaaa tagcccacat caaacggttt ttaggtcttc agtacgccat
    12361 agcaccagtg cacgagtttt ctcatcagcg caccaatggc acacattttg gccttacctc
    12421 tcaggcataa tcgctcatac atcgctttca tcaatgggtt aaatctcaga ccacacaacg
    12481 aagccagata cagtttcgcc cggatttccg gcggaccaat ttttgacagt ttcggacacc
    12541 ctctgaccga ggtgccggaa tatttttcaa cgggaacgac acctaaaaat gcagccgcct
    12601 gttcacgtat aggaaattga aaaaccagac gatacc
//
LOCUS       DAJTYD010000108        12629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTYD010000108 DAJTYD010000000
VERSION     DAJTYD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.108"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..444)
                     /locus_tag="OO585_002735"
     CDS             complement(<1..444)
                     /locus_tag="OO585_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6630270.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGV"
     gene            complement(474..1190)
                     /locus_tag="OO585_002736"
                     /pseudo
     CDS             complement(474..1190)
                     /locus_tag="OO585_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1499..2275
                     /gene="exuR"
                     /locus_tag="OO585_002737"
     CDS             1499..2275
                     /gene="exuR"
                     /locus_tag="OO585_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6630271.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2620..3282
                     /locus_tag="OO585_002738"
     CDS             2620..3282
                     /locus_tag="OO585_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6630272.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3286..3669
                     /gene="mzrA"
                     /locus_tag="OO585_002739"
     CDS             3286..3669
                     /gene="mzrA"
                     /locus_tag="OO585_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6630273.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3816..4184
                     /locus_tag="OO585_002740"
     CDS             3816..4184
                     /locus_tag="OO585_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6630274.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4222..4527
                     /locus_tag="OO585_002741"
     CDS             4222..4527
                     /locus_tag="OO585_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6630275.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4530..4934
                     /locus_tag="OO585_002742"
     CDS             4530..4934
                     /locus_tag="OO585_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6630276.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4924..5223
                     /locus_tag="OO585_002743"
     CDS             4924..5223
                     /locus_tag="OO585_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6630277.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5409..5801
                     /locus_tag="OO585_002744"
     CDS             5409..5801
                     /locus_tag="OO585_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6630278.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5871..6774
                     /locus_tag="OO585_002745"
                     /pseudo
     CDS             5871..6774
                     /locus_tag="OO585_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7067..7432
                     /locus_tag="OO585_002746"
     CDS             7067..7432
                     /locus_tag="OO585_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6630279.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7674..8030
                     /locus_tag="OO585_002747"
     CDS             7674..8030
                     /locus_tag="OO585_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6630280.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8081..8973)
                     /gene="yhaJ"
                     /locus_tag="OO585_002748"
                     /pseudo
     CDS             complement(8081..8973)
                     /gene="yhaJ"
                     /locus_tag="OO585_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            9078..9779
                     /locus_tag="OO585_002749"
     CDS             9078..9779
                     /locus_tag="OO585_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6630281.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9802..9966
                     /locus_tag="OO585_002750"
     CDS             9802..9966
                     /locus_tag="OO585_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630282.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10100..11410)
                     /locus_tag="OO585_002751"
     CDS             complement(10100..11410)
                     /locus_tag="OO585_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6630283.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11438..12629)
                     /locus_tag="OO585_002752"
                     /pseudo
     CDS             complement(11438..12629)
                     /locus_tag="OO585_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3252 a         3193 c         3004 g         3180 t
ORIGIN      
        1 aacacctttg gtggtttgag caaacatcag gtttttagcc agagaaccgt tagttaacgc
       61 atcggaagca aagattttgg ttgcggtaaa agtggttgcc gcatcagctt taatttcttc
      121 accgccccag gttactgtca gcggaacaat cttgctagca tccaggcttt gaaccagttg
      181 cgccggggaa actaactgca tttttacatc tttggtaacg tcgttagacc agattttggt
      241 catcaactgg taagattgca gaccctgccc cggcagatat tgcatttcta ctgctttcgg
      301 caacgcggtg ttatcggtct gtgtcatatc cagagcggca tcaacgttgg ccgttacagt
      361 aatatccttt tgtactgcca atgcggagcc tgcaacagaa aacatcgctg cgaccagaag
      421 agattttgca aatacctttt tcattactat tccttcgttt attacaatga taattaatgg
      481 accgccagtt caaactcgcc attatctttg gtgatccagt tattatattt gacaacgatt
      541 ttgtcgtagc cagatatgct ttcaagattt ctttcctgac cagggaagat gtttttattt
      601 acggtctgta tcttacaatt ttctttgcta ttatttttac aataagcgat cgttaaaatc
      661 tttaagcgtt gattccctgc attaactaat ttttgggcag catttacttc taacttagca
      721 ataggttgct gaggtggaac actaacaaca atcccccaaa ttaagctaac agttaactgc
      781 atagagaaat cttttttatc tgtggcaacg tcatcaatac tgggaaccgc ttcaaattta
      841 acgcgttaat ttttttcttt ttgctctggt tccattgcaa caaagcgcac agttttgcta
      901 ctaccggctg gtaaagcgaa tttaggtggc ataacaacca gatgatttgc atcaccggat
      961 ttaatctcaa cttcattttc ctgaggcgtc gatggattat cgatacggaa tactgtcgcc
     1021 ttgatgtact gaacttcatt acttttagat attacgcgaa cgttgccttc atcctgacta
     1081 ttaatggaaa cagccatagg ataaacagtc atatttgact gtgccaaaaa aggagcgaac
     1141 acgaataaaa gcggcgcaag ccgtgataag agattaattt tcgccaccat ataaatttga
     1201 tcttacaata ttgtttccaa aaagttggtg tattagagat gtagatttat tgtaagtcaa
     1261 tataaaaaag catttttagt taatattaaa tttaaaggct gggaattaac ttgctgaaat
     1321 ttagtagctt tcacaatgac gatacttatt ttttaggaat ttataaatat tcacaatatt
     1381 aattcaatac attaattcaa tacattaatt caatcaccaa atttatatat gaccaatggc
     1441 atacccatga ataaagtggt atgataactt cgtccgcatt ccactccggg agacgcatat
     1501 ggaaatcact gaaccacgcc gtttgtatca acaacttgcc gctgacctga aagagcgcat
     1561 tgaacagggc gtctatctgg tgggtgataa actgcctgcc gaacgcttta ttgccgatga
     1621 aaagaacgtc agccgaacgg tagttcgtga agccatcatc atgctggaag ttgaaggcta
     1681 tgtggaggtg cgtaaaggtt caggtattca tgtggtttcc aaccagccgc gccatcagca
     1741 ggcggctgac aataatatgg aattcgccaa ttacggtccg tttgagttgc ttcaggctcg
     1801 ccagctcatc gaaagtaata ttgccgagtt cgcggcaact caggtaacga aacaagacat
     1861 catgaaactg atggccattc aggaacaggc gcgcggcgaa caatgcttcc gtgattccga
     1921 gtgggattta cagttccaca ttcaggtagc cctggcgacg cagaactccg ccctggcggc
     1981 catcgttgaa aaaatgtgga cccagcgtag ccataacccg tactggaaaa aactacacga
     2041 acacattgat tcccgtaccg ttgataactg gtgtgatgac cacgatcaaa ttctcaaggc
     2101 gttaattcgc aaagatcccc atgctgctaa gctggcaatg tggcagcatc tggaaaacac
     2161 caaaatcatg ttatttaacg aaaccagcga cgatttcgag ttcaatgccg accgctatct
     2221 attcgccgaa aacccggtag tgcatctcga tacggccacc agtggcagta aatgaaactc
     2281 ctgtccgaca ggcgctgcga cgcgcctgtt cgcttcgtcg gtaagcgaaa agtataaagt
     2341 gtcagcctgt gtaaatcctc tcgccaccct cccctgcatt cagcaaaatc agactccacg
     2401 gacatgtaat tttgataacg aacaacgttg acctttgtta caattagatt caatttgaat
     2461 ttatgttttt gaatgctttc ttatctcacg atttaacagg gaatagttca ggctgtgttg
     2521 atgtatcaaa cccgcagaac ataccaaaac agcaataaca ttgcggtagt gcatctttta
     2581 aaaccagcgt ggcgttaacc gattcaccag gaataatgaa tggaactttt gacccaattg
     2641 ctgcaagccc tgtgggcgca ggattttgaa accctggcca atccatcgat gattggcatg
     2701 ttgtattttg tcttgtttgt aattttgttc cttgaaaacg gcttgcttcc agcggccttt
     2761 ttaccgggcg acagtttact ggtattggtc ggcgtgttga ttgcgaaagg cgcgatgggt
     2821 tacccgcaaa cgattctgct gctgaccgtt gccgccagcc tcggctgctg ggtcagctat
     2881 attcaggggc gatggctggg caatacccgc accgtacaaa actggctatc tcatttaccc
     2941 gcgcattatc atcaacgcgc acaccatctt tttcataaac acggtttatc ggcgctgtta
     3001 attggtcgct ttattgcgtt tgtcagaaca ctgctgccga cgattgccgg gttatcaggg
     3061 ctgaataacg cgcgctttca gtttttcaac tggatgagcg gtctgctgtg ggtattgatc
     3121 ctgacaactc tgggttacat gctcggcaaa acgccggtat ttttaaagta cgaggaccag
     3181 ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg tgtttggcct ggcaggttct
     3241 ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt aagggatgca aatacctcgc
     3301 atgtcgcttc gccagctagc ctggtccggc actgttttac ttctggtcgg cacgctgttg
     3361 ctggcctggt ccgcggttcg ccagcaagag tctacgctgg cgattcgtgc cgttcatcaa
     3421 ggcacaacga tgccagacgg tttttcgatc tggcatcacc ttgacgctca tggcattcct
     3481 ttcaaaagta tcacccccaa aaacgacact ctgttaatta catttgactc cagcgaccag
     3541 agcgccgccg caaaagcggt cctcgacaga acattgcccc atggctacat cattgcgcag
     3601 caggacaata acagtcaggc tatgcagtgg ctgacccggt tacgggataa ctctcatcgc
     3661 ttcggataac ttcctgttat ccgaaacata tcactcactt tggtgatttc accgtaactg
     3721 tctatgatta atgaggcggt ggttaacact accgcttcgt tgtctggaca atcgttcctt
     3781 tgtaacaggt ccgaatcaca atggaaggtt caagaatgaa ataccgcatc gctttagctg
     3841 tttctctctt tgctcttagt gccggtagtt atgccactac cctgtgtcag gaaaaggagc
     3901 aaaatatcct taaggagatc agctatgccg aaaaacacca aaaccagaat cgtattgacg
     3961 gtctgaataa agccctgagt gaagtccggg ccaactgttc agatagccag ctgcgtgccg
     4021 atcatcacaa gaaaatcgca aagcagaaag atgaggtggc ggaacgccag caagatttag
     4081 ccgaggcgaa gcaaaaaggc gatgccgata agattgccaa acgcgaacgg aaactggcag
     4141 aagcgcagga agagctgaaa aagctggaag cgcgcgacta ctaactcaca atagtcacta
     4201 cttactcacc tggagaaaac tatgtcgaaa gaacacacta cggaacatct gcgtgctgag
     4261 ttgaaatccc tttccgatac gctggaagag gtgcttagct catctggcga gaagtcgaaa
     4321 gaagagttga gtaagattcg tagcaaagcg gagcaggcac tgaaacagag ccgttatcgc
     4381 ctgggtgaaa ccggtgatgc catcgccaaa caaacccgtg tcgcggcggc gcgtgctgat
     4441 gagtatgtgc gtgaaaatcc gtggaccggc gtgggaattg gcgctgcaat cggtgtagtg
     4501 ctcggcgttc tgctgtcgcg tcgttaatta tggcggacac tcatcacgcg caagggcccg
     4561 gtaaaagcgt tctgggcatc gggcagcgaa ttgtttccat catggttgaa atggtggaaa
     4621 cacgtctgcg gctggcggtg gtggagctgg aagaggaaaa agctaatctc tttcaacttt
     4681 tactgatgct gggcctgacg atgcttttcg ctgcatttgg tcttatgagc ctgatggtgc
     4741 taattatttg ggcggttgat ccgcaatatc gcctgaatgc gatgattgcc accaccgtgg
     4801 tgttgctgct actggcactg attggcggta tctggacgtt acgtaaatcg cgtaaatcca
     4861 ccttgctgcg ccatacacgc catgagctgg caaacgatcg gcaactgctc gaggaggagt
     4921 cccgtgagca gtaaagtcga acgtgaacga cgtaaggcgc aactgcttag ccagatccag
     4981 caacaacggc tggatctttc cgccagtcgt cgtgaatggc tggaggcaac aggcgcttac
     5041 gatcgtcgct ggaatatgct gctaagtctg cgctcctggg cgctggttgg cagtagcgtg
     5101 atggcgatct ggacgattcg ccatcctaat atgctggtcc gctgggccag acgcggtttt
     5161 ggcgtatgga gcgcctggcg tctggttaaa acgaccctca agcagcaaca gcttcgcggt
     5221 taacttccct ctggccggag ccattccggc cctatcactc aaattttttg aagatttttg
     5281 acagttttcc ttgctaacaa tcatcattca ccacgtttat gattctctcc atcgacagca
     5341 acgacgctaa taccgcgccc ttgcacaaaa aaacaatcag cagcctgagt ggcgcagtgg
     5401 agagtatgat gaaaaaatta gaagatgttg gtgtactggt agcgcgcatt ttaatgccga
     5461 ttctgtttat taccgcaggc tggggaaaaa tcaccgctta ctctggtacc caacaatata
     5521 tggaagcaat gggtgtcccg ggttttatgc tgccgctggt gattctgctc gagttcggtg
     5581 gtggtctggc aatcctgttc ggtttcctga ctcgcaccac agccctgttt actgcgggct
     5641 ttacgctgct gacggcattt ttatttcaca gcaagtttgc tgaaggcgtc aactcgctga
     5701 tgttcatgaa aaacctgaca atttctggcg gattcctgct gctggcaatt accggtccgg
     5761 gcgcgtatag catcgaccgc ctgctgaata aaaagtggta agcacgcgct atacttaacg
     5821 ataaaaaacg aggaggaagc tcctcgtttt tgctattgga ggagagaaaa atggggcaac
     5881 tgattgacgg cgtctggcat gacacctggc cccactggca caggcggttt tatcgcagag
     5941 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     6001 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     6061 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     6121 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     6181 actgttcccg tgctgtggga caaaaagaac cacaccattg tcagcaacga atcagcagaa
     6241 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     6301 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     6361 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     6421 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     6481 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     6541 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     6601 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     6661 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     6721 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     6781 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     6841 gtgaacgcct tatcaaacct acaaaacgtt tatattcaat atgttaaaat gaccgcgtag
     6901 gccagataag cgtagcgcat ctggcgattt tgcgcttgtc atcagtctta gcgcaccttc
     6961 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     7021 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     7081 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     7141 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     7201 ctggcaacgc gccggtggcg aaggcatcct gacgacaatc tacggtattc tggtgttttt
     7261 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     7321 actgttgttc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     7381 gggtacgcca ggcgaaaacc gctttggacc agatccgaaa ctggaacctt aaaacaaatc
     7441 tgctcgccgg atgtctgact catccggctt ttatcatcat gttgatgaat gaataattta
     7501 ctaatgttta tttaaaatat ttcaatgtcg ttatataaat tacctataaa aaacaaccat
     7561 gtatttatct taaatatcaa aacctttata acatccgaaa aagaaagata cgaatagcat
     7621 ttacacaata cccggaatag actttcgtca cttttaatta aagggatgtt tttatgcagt
     7681 ggtatttagc cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt cgtaaagagt
     7741 actggatgtt tactctgatt aacgccatcg tcggcgctat tatcaatgtc attcaattga
     7801 ttttaggttt agagtttcca ttcctttctc tcatttacct ggcagcaaca atcattccgg
     7861 tcattgctct gtgtgttcgt cgtctgcacg atactgaccg ttcaggtgtg tgggcgcttc
     7921 tctatctggt accgatcatt ggttggttag ttctgtttgt atttgcctgt ttagaaggca
     7981 attctggcag caatcgttac ggaaacgatc ctaagtttgg ttcaaattaa tcttagaatt
     8041 ggggcgatat ttcgcccctt tttattaaca ataataaata ttattttccg ctaaaaagtt
     8101 tgggaatttc ccgcagacac caggatttcg cttcccccat actgtcacga cgccaggcca
     8161 taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcaa
     8221 tatctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaatgcct
     8281 gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc agctgtacgg
     8341 tcaacaccgg gcgctcacga gcggtatccg ctaccgcaat tccacgatat ttcacgcgcg
     8401 tcacttcaga taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga
     8461 cgttcattaa ggtatagagc ttgcgcgagt tgatctccga cgaggaacga aaatgcatat
     8521 ccggcgcgat aacgatatcc gcccgcccct gctccagtcg ttcccacgcc cccgccagca
     8581 cttcggtgat gattgccagt tgggtattgg cttttgccgc cagtttgtcg attaacggga
     8641 aaaaggcagg tgtcggtacc agcgcctcgg tcacaatggt gagatgcgtt tcccaaccgc
     8701 gcgcgagggc ttccgcatcg gtagtcagtt tatctgctgc ttccagcaaa acgcgccctc
     8761 gctccagcaa catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca
     8821 gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc
     8881 gtcccagctc atccgccgcc gccgcaaaac tgccccgacg atcgcatcca taacccgtag
     8941 tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga aatttgaaca
     9001 taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata aaaggaagta
     9061 aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga caagcagact
     9121 acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac ccgaaattgt
     9181 taggctatgc ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt gccgcctttc
     9241 agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga gaagcagagt
     9301 atcgcgacag cgaaggcaat catgttcagg ccagcgccgg tgaggcgttg ctgctctcta
     9361 cccagccggg tgtcagctac agcgaacaca atctcagcaa agacaaaccg ttaacacgaa
     9421 tgcagctttg gctggacgcc tgcccgcagc gagagaatcc gctgattcaa aaactggcgc
     9481 ttaatatggg caagcagcaa ttaatcgcct cgccagaggg tacgatggga agcctgcaat
     9541 tacgccagca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc
     9601 agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat gcgcttacgc
     9661 atcatgaaga gaaagcggcg ctgacctgcg gtgatggggc gtttattcgt gacgaggcta
     9721 acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg cctgtctagt
     9781 tgtttttaca gggagatgat gatgagtaaa aaatcggcca aaaagcgcca gccagtgaag
     9841 cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga agagatgttg
     9901 agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga tgaagctacc
     9961 gcgtaaagac aggttgccgg atgcggcgaa aacgccttat ccggcctaca aaatccaaca
    10021 acattaacga attaaacaac ccgtaggccg gataaggtgc gccagcatcg catccggcac
    10081 aatactcaaa acctggcgtt tatctggcct tgctcgccat aatctcgata atctgccgat
    10141 ccgtttgctg catcgaatgg cttgctaacg cacacaggtt ggcaatcgac tgctcaacat
    10201 catgcgccac gatcccttca ttgccggtca cggcggtatc atccagcgcc attaacaccg
    10261 ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc gacgcaccat
    10321 cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag atagtttcat
    10381 aacgcccatc caccagccat gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg
    10441 cacaaagtgc agacaaacgc ggtaactggt tatggatgta aattgcgctc aaatgagaaa
    10501 gcatcagcgc acgcgccagt cgttcatcat ccgctccgaa gtgttctgct acaaccacca
    10561 caggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcata gccggaagcg
    10621 tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg gatgaagaga
    10681 gatctttcgc cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca atatgcagcc
    10741 cccacttacc gctcaaacct tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa
    10801 taaagcggat cgccgcaaac gggacttcat tgacgaactt caggatctca gccagcgtcg
    10861 ttctggaaag caccgtcagc ggagactctt gctcgccctc tgccacacac gcctgctggg
    10921 taaacaccac actattgtgc gtctcaatat gcacaatgtt ggtatgcccg ccgacgatgg
    10981 tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg atttcattgc
    11041 aaggttcctg gatcttaacg gagactttcc ccgccgccag cagtgctttg gcatcggcaa
    11101 ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc gttggcattt ccacctaacg
    11161 cccccagcgc cgccgcaatc ggcagcccca ccattcccgt gccgggaacg gtgacgccca
    11221 gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc ggaccttcca
    11281 gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta catcccagcg
    11341 ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga tttaaagtcg
    11401 aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc aggaacggag
    11461 aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct ttgtatttgt
    11521 gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc atgccgaaaa
    11581 tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg gcgctccagg
    11641 ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag attttcgtta atcttctcgg
    11701 caggcatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg cgaaagccta
    11761 aatagacgcc aaagaatgca gtcatgacag caaagatatt gagaatgacg ctgaccactt
    11821 tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc gcggaaatat
    11881 tctgctcgta ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaac gacacggcgt
    11941 agaaaaagac ggtgacaaat aaaatgccaa acgcgatatt cattgcccgc aatgctttat
    12001 gccgcgccac ttcaattgat ttttcccgcg agcgataaga gatcaccatc gggcttaacg
    12061 tctggataaa cagaatcgac gtcagggtaa acggcagcgt aataatggcg tttttcacca
    12121 gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc accatcgaca
    12181 cgcccagcgc agcgactacc agcagcttgg tcagcaccat gccggtcgaa attttgaata
    12241 acaatttctc gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc agaccataaa
    12301 agggactgtc tgacagcacc ccttccgtca cgccgaaggt atgcagatag gaagcactat
    12361 cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg aaatagagcg
    12421 cacctaacag gatgccccag tttttaccta aataaccgct aatgacactc gggtaatctt
    12481 tacattctgt gtgattctgc cagcgtatta ataaacaacc gctgaaacag atacattgcc
    12541 gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc gacctgcacc
    12601 gggagaaaaa caatccccgc gccaatcgc
//
LOCUS       DAJTYD010000109        12605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTYD010000109 DAJTYD010000000
VERSION     DAJTYD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.109"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(17..523)
                     /gene="mug"
                     /locus_tag="OO585_002753"
     CDS             complement(17..523)
                     /gene="mug"
                     /locus_tag="OO585_002753"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6630284.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            648..723
                     /locus_tag="OO585_002754"
     tRNA            648..723
                     /locus_tag="OO585_002754"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:681..683,aa:Ile,seq:cat)
     gene            complement(777..1541)
                     /gene="nfeF"
                     /locus_tag="OO585_002755"
     CDS             complement(777..1541)
                     /gene="nfeF"
                     /locus_tag="OO585_002755"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6630285.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1829..2452
                     /gene="nfeR"
                     /locus_tag="OO585_002756"
                     /gene_synonym="yqjI"
     CDS             1829..2452
                     /gene="nfeR"
                     /locus_tag="OO585_002756"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6630286.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2606..4126)
                     /gene="aer"
                     /locus_tag="OO585_002757"
     CDS             complement(2606..4126)
                     /gene="aer"
                     /locus_tag="OO585_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6630287.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDVSADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4543..5922
                     /gene="ygjG"
                     /locus_tag="OO585_002758"
     CDS             4543..5922
                     /gene="ygjG"
                     /locus_tag="OO585_002758"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6630288.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5964..6296)
                     /locus_tag="OO585_002759"
     CDS             complement(5964..6296)
                     /locus_tag="OO585_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6630289.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6515..7498
                     /gene="ebgR"
                     /locus_tag="OO585_002760"
     CDS             6515..7498
                     /gene="ebgR"
                     /locus_tag="OO585_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6630290.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7682..10774
                     /gene="ebgA"
                     /locus_tag="OO585_002761"
     CDS             7682..10774
                     /gene="ebgA"
                     /locus_tag="OO585_002761"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6630291.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLGNLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10771..11220
                     /locus_tag="OO585_002762"
     CDS             10771..11220
                     /locus_tag="OO585_002762"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6630292.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11283..>12605
                     /locus_tag="OO585_002763"
     CDS             11283..>12605
                     /locus_tag="OO585_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6630293.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVG"
BASE COUNT         3014 a         3407 c         3171 g         3013 t
ORIGIN      
        1 ttttcggtgg cgttttttat cgcccacgca ctaccagcgc ctggtccagc tcgcgatacg
       61 cttcaaccag tttctctagt gaaacgcgac ttaaaccgct gggatttggc agcacccaaa
      121 tctgcgtcga accaatggtg agcgtttgtt tcccccactg tgcaccgcgc tggctgaatc
      181 cctgttcata tgcttgtttg cccagaatcg ccaacgcctg cggctgataa tcttcaattt
      241 tttcaatcag cttacgccca cctgcgtgca gctcctgctt tgaaacttca ttggcttgca
      301 ccgttggacg gtctaccagt ttggtgacgc cacaacgata atccagcaga tgctgtgcct
      361 cctgcggctt caactgacgg tcggtaaacc cggcctgata tatcaccttc cagaagcgat
      421 ttgctggatg agcaaaggga aaaccagtcc cggcggatga aagcccaggg ttgataccgc
      481 aaaacacgac ccgtaaccct ggagccaaaa tatcctcaac catatttact cctttctaac
      541 atcaccagga aaagtataac ggattgaaaa tacattgttt ataaaaacag caggcgcacg
      601 gtaatggctg gattgcgaca cggagttact ttataatccg ctaccatggc cccttagctc
      661 agtggttaga gcaggcgact cataatcgct tggtcgctgg ttcaagtcca gcaggggcca
      721 ccagatatag caaaggctga cgagaaatcg tcagcctttt tctttttata tctcagttac
      781 tttgcgtgcc agtaagccgc tgcacgtacc cgctgtgggt catactgttc cgcttcaaag
      841 cggcggctta aattcttaac gactttacct tcgccggtta tccagatgaa gtaatcatcg
      901 gcagggattt gcatctgcgc cagacgcgca tctaccgcct gctcatcatg tgccagccat
      961 tcgatattaa aaccatcaag gtgcgcgaga taatcctgac aggcgttatc ccgcacgcta
     1021 accagcgcac taacttgcgg tttaacggca agtttgctca acgtttccag gcggcggcgc
     1081 agtgcgggca ttccggattc atcgcagaca tacagctgat acgcgtaatc ttccggcacc
     1141 accagcgaac cgcgcggacc agccaccgta agtttatcgc ccggttgcgc ctgcatcgcc
     1201 cagccgctgg cgaccccacc gtcgtgaata aagaaatcga tcgccagttc atggcgtagt
     1261 tcgtcataca gcggcgtata gtcacgcgac ggtgggcgtg gtccttccgg ccagacgatg
     1321 ccctcttccg ttaccgttgg cggcacaaag tgagcgtcag gttgaggaaa gaagagtttg
     1381 ctgtgatcgt caaagccgcg cgatgtaaaa ccgtccagcg cctcgccgcc gaggacaatg
     1441 cgctgaaaac cggcgctgat gcgctcaacg cgtaacacag tcagttcacg gaagcgcaga
     1501 tcattgcgaa cgcgctgcgg gtagcggggg gagttattca ttgttatcgc cttcgtgatg
     1561 gtaatcagat atatctaaat aattttcgta aatgataatg attgttaatc atgataaatg
     1621 caagcgatta gtagaactga tatgtctata gtctgataag acgaaccgcc tcttctcagg
     1681 catcattact caacgccgga tgcggcgtga acgccttatc cggcctacgt gtgagatgag
     1741 tccacattta aaaatcattt ttattcttgc aagagtgctc atatcagata taaattagat
     1801 atatctaatc aagaaaaagg aggctgatat gagccatcat cacgaagggt gttgtaaaca
     1861 tgaaggccag ccacgccatg agggctgctg caaaggtgag aagtcagaac acgagcactg
     1921 cggacacggt caccagcatg aacacggtca atgctgcggt ggtcgccacg gtcgcggcgg
     1981 cggtcgtcgg caacgtttct ttggtcacgg tgaattgcgt ctggtgattc tggatattct
     2041 ctcgcgcgat gacagccacg gttacgaatt gattaaagcg attgagaatc taacccaggg
     2101 gaattacacc ccaagcccgg gcgtcatcta cccaacgctg gattttctgc aggagcagtc
     2161 gctgattacc atccgcgaag aggaaggtgg taagaagcag attgcgctga ccgaacaagg
     2221 cgcgcagtgg ctggaagaaa accgcgaaca ggtggagatg attgaagaac gcatcaaagc
     2281 gcgttgcgtt ggcgcggcgc tgcgccagaa cccgcaaatg aagcgggcgc tggataattt
     2341 taaagcggtg ctggatttac gcgtcaacca gagcgatatc agtgatgcac aaataaaaaa
     2401 gatcattgcg gtgatcgacc gcgccgcttt tgatattacg caactggatt aatcgccgca
     2461 tccgccagtg gcgcggtgca attgccggat gcgacgcttg acgcgcctta tccggcctac
     2521 acccactaca caccccgcag gcctgataag atgcgccagc atcgcatcag gcattgtgct
     2581 ccaaccgccg gatccggcat accgattaat gcagcaccgt caccgcgtct tccagtcggc
     2641 tggcgcggtg tttcaccatc gccgacacct gcgcactctc ttccaccagc tcagcatttt
     2701 tctgggtgat caggttaagc tcatccactg cacgggtcag gctggaaagc ccatcggcct
     2761 gttccagcgt tgaatggcta atctgggcga tcaactgggt gacgtttttc acctgtgcca
     2821 caatatcttc catcgtccgt ccggcggcgt gtacctgctg cgaaccggat tgcaccttat
     2881 cagcactgac atcaatcagc ttgcgaatat cgttggcagc attagcgctg cggctggcta
     2941 aatgacgcac ttcccctgcc accactgcaa aacctttgcc ctgttcgccg gcacgcgccg
     3001 cttccaccgc cgcattcagg gccagaatat tggtctgaaa cgcaatatcg ttaatcagcg
     3061 aagtaatggt gccaatgcgc tgggtactgt cggcgatatc gtccattgtc ttgatcaccg
     3121 tggtcatcgc ctccccaccc tgcactgccg cattactggc gatgatagaa agtttatcgg
     3181 cagccgacgc cgtggcagag ttctgtttca ccgacgccgc catttggttc atggtcgcca
     3241 ccgtttgctg aacgttatca actgtctgct gggtatgttc gttcagttca tcggtgcctt
     3301 tcgccagcgt ctcactgcca tttctgacac tggacacctg gcttgagaca tcgttaatta
     3361 gccaacggca catcaggcca agctgcccta ccgcgcgtaa tgtcagcccc agctcatcgc
     3421 tgcgattcag gtgctcaaca ctattacgct ctccggtcgc caccttcagt gcctgacggg
     3481 cgacattttc tatcgggcgc acaatctgcc attcaaaaca agcgcttgcc aacaatacca
     3541 ctaacacaca gaggaaatac gtcaccaccg gggcagcaac aaaccaaagc atggccgcca
     3601 gcaagataaa catcagggtc atcactccac gcgcccgcca gcgaagcggt aatgaaggca
     3661 gtttacccag ccagccttta cgcaccacca ggcctttatg aatacgctta ctggtacgtc
     3721 cggcgttcag cgctttgtac agcggctcca ccgccgcaat ctcttcatcc gtcgcccggg
     3781 tacgaatcga catatagcca ctgatttttc cctcgcgcac catcggtacc gcattggccc
     3841 gcacccaata atggtcgcca tttttgcggc gatttttcac gatgccgctc cagggctccc
     3901 cttttttcag ggtgaaccac atatccgcaa acgccgcttt tggcatatcc gggtgacgca
     3961 ccatgttgtg cggctgccct tgtaactctt gcaaggtaaa gccgctcacc tgcacaaaag
     4021 tgtcattagc atgagtgata tagctttgca gatcggtagt ggacatcaga gtggtatcgt
     4081 ccgccagcgg ggtattttgc tgggtgacat acggatgaga agacatgttc gcgtcctatg
     4141 caggttatat ggttgttaac ttcttgtcag agtttatgtc ggccccgctg cggttatctt
     4201 taaccgatta atttgattta gatcgcaatt tgcgattaaa gcacaaatct aattccttga
     4261 tttaaaatac tttcactctg ttactatacg aaaacgttaa ttatcttgcc caaaatcagg
     4321 caattatcgc cctgaaaacg tgcatttgca cagcaatcat caaatccata cccgacaaaa
     4381 accgtgcaaa ataacaacga atgttaacaa atagcattaa atactgcgca aatgataacc
     4441 acatttgtgt ttatcccgat tttcgcgatc gcagccggag tggcgcaatc cctgcaatac
     4501 ttaaatcggt atcatgtgat acgcgagcct ccggagcata ttttgaacag gttaccttcg
     4561 agcgcatcgg ctttagcgtg cagcgcccac gccctgaatc tcattgagaa gcgaacgctg
     4621 gatcatgagg agatgaaagc acttaaccga gaggtgattg aatacttcaa agagcatgtc
     4681 aatccggggt ttttagagta tcgcaaatct gttaccgccg gcggggatta cggagccgta
     4741 gagtggcaag cgggaagttt aaatacgctt gtcgacaccc agggacagga gtttatcgac
     4801 tgcctgggag gttttggaat tttcaacgtg gggcaccgta atccagttgt ggtttccgcc
     4861 gtacagaatc aacttgcgaa acaaccgctg cacagccagg agctgctcga tccgttacgg
     4921 gcgatgttgg cgaaaaccct tgctgcgcta acgcccggta aactgaaata cagcttcttc
     4981 tgtaatagcg gcaccgagtc cgttgaagca gcgctgaagc tggcgaaagc ttaccagtca
     5041 ccgcgcggca agtttacttt tattgccacc agcggcgcgt tccacggtaa atcacttggc
     5101 gcgctgtcgg ccacggcgaa atcgaccttc cgcaaaccgt ttatgccgtt actgccgggc
     5161 ttccgtcatg tgccgtttgg caatatcgaa gccatgcgca cggctcttaa cgagtgcaaa
     5221 aaaaccggtg atgatgtggc tgcggtgatc ctcgaaccga ttcagggtga aggtggcgta
     5281 attctgccgc cgccgggcta tctcaccgcc gtacgtaagc tatgcgatga gttcggcgca
     5341 ctgatgatcc tcgatgaagt acaaacgggc atggggcgca cgggcaagat gttcgcctgc
     5401 gagcatgaga acgtacagcc ggatatcctc tgccttgcca aagcgctcgg cggcggcgtg
     5461 atgccgattg gcgcgaccat cgccactgaa gaggtgtttt cagttctgtt cgacaaccca
     5521 ttcctgcata ccaccacctt tggcggcaac ccgctggcct gtgcggcggc gctggcgacc
     5581 atcaatgtgt tgctggagca gaacttactg gctcaggctg agcaaaaagg cgatatgttg
     5641 ctggacggtt tccgtcaact ggcgcgggaa tatcccgatc tggtacagga agcgcgtggt
     5701 aaagggatgt tgatggcaat tgagtttgtt gataacgaaa tcggctataa ctttgccagc
     5761 gagatgttcc gccagcgcgt actggtggcc ggaacgctca ataacgccaa aacgatccgc
     5821 attgaaccgc cactgacact gaccattgaa cagtgtgaac tggtgatcaa agcggcgcgt
     5881 aaggcgctgg cggccatgcg agtaagtgtc gaagaagcgt aatatcaaat cggatggcga
     5941 tacgacgtcg ccatccgatc tttttatacg acgcgtacgc ccgcaggcat catccgctcc
     6001 ggcgttaaca acacgctttc actgccgtca tcggtttccg cacacaacag catgcattcc
     6061 gacgtttcac cacgcatttt cgctttttgc agattgcata agaccaccac cgttttcccc
     6121 atcagctctt cttcgctgta gtacggcacc aggctggtca cggtttgcag tgttttttcc
     6181 cccacatcaa cctgtacgat gtacagcttg tcggcgtttt catggcgttt cacttccaca
     6241 atctttccga cgcgcatttc cagacgtgca aaatcagcgt aagccacggt ttccattgtt
     6301 tccttccttc gggtaaaatt ttactaaact atagcaaaag tttttctcaa tcctgtaggc
     6361 taaaaatgga gaatgcaggc gtgatcacat tcgtgagccg ctgtgttacc gttacagcgt
     6421 caaagaaacg cgctttattt actgaaaaca ggtgacccga taagtacttc ctctacaatg
     6481 ggggcgcaca tcagggaaag taaaaaaggt aaacatggca acactaaaag acatcgcaat
     6541 cgaagctggc gtatccctgg cgacagtatc cagggtctta aatgacgatc cgacattgaa
     6601 tgtgaaagaa gagacgaaac atcgcattct cgagatcgcc gaaaagctgg agtacaagac
     6661 cagtagtgcc cgcaaactcc agacaggtgc agtcaaccaa caccatattc tggctatcta
     6721 cagctaccaa caggagctgg agatcaacga tccttactat ctggcgatcc gccacggcat
     6781 tgaaactcag tgcgaaaagc tgggcattga gctcaccaac tgttatgaac acagcggatt
     6841 accagacatt aaaaacgtca ccggtatttt aattgtcggc aaacccacgc ccgcccttcg
     6901 cgccgccgcc tgtgcgttga ccgacaatat ctgttttatc gactttcacg aacccggcag
     6961 cggttacgat gcggtggata tcgatctggc acgcatcagt aaagaaatca tcgacttcta
     7021 tatcaatcag ggcgttaatc gtattggttt tattggcggt gaagatgagc caggcaaagc
     7081 ggatattcgt gaggtcgcct ttgcggaata tggccgactg aaacaagtgg tacgcgaaga
     7141 ggatatctgg cgcggaggct tttccagttc gtcgggttat gaactggcaa aacagatgct
     7201 ggcgcaggaa gactatccga aggcactgtt tgttgcttcc gattccattg ctatcggcgt
     7261 actgcgggca attcatgaac gaggcctgaa catcccacag gatatttcgc ttatcagcgt
     7321 taacgatatc cccaccgcgc gatttacctt tccgccgctc tccaccgtgc gcatccattc
     7381 cgagatgatg ggaagtcagg gcgttaacct ggtgtatgaa aaagcccgcg atggtcgcgc
     7441 gctgccgctg ttagtcttcg ttcccagcaa attaaaactg cgcggcacga cccgttaaat
     7501 ccccttacac actgtccggc aatcgttttt gccggacagt gctgccgttt attttcgtga
     7561 tccagttaaa gtaaatgcat ttatctgcta ctttttagta aaaattttac tgaactcccc
     7621 agcaattaca caaactacca tcaccatgaa tggttccgat ttctctctac cgggaggccc
     7681 tatgaatcgc tgggaaaaca ttcagctcac ccacgaaaac cgacttgcgc cgcgtgcgta
     7741 ctttttttca tatgattctg ttgcgcaagc gcgtaccttt gcccgcgaaa ccagcagcct
     7801 gtttctgccc ttaagcggtc agtggaattt ccactttttt gaccatccgc tgcaagtacc
     7861 agaagccttc acctctgagt taatggctga ctgggggcat attaccgtcc ccgccatgtg
     7921 gcaaatggaa ggtcacggca aactgcaata taccgacgaa ggttttccgt tccccatcga
     7981 tgtgccgttt gtccccagcg ataacccaac cggtgcctat caacgtattt tcaccctcag
     8041 cgacggctgg cagggtaaac agacgctgat taaatttgac ggcgtcgaaa cctattttga
     8101 agtctatgtt aacggtcagt atgtgggttt cagcaagggc agtcgcctga ccgcagagtt
     8161 tgacatcagc gcgatggtta aaaccggcga caacctgttg tgtgtgcgcg tgatgcagtg
     8221 ggcggactct acctacgtgg aagaccagga tatgtggtgg tcagcgggga tcttccgcga
     8281 tgtttatctg gtcggaaaac acctaacgca tattaacgat ttcactgtgc gtaccgactt
     8341 tgacgaagcc tattgcgatg ccacgctttc ctgcgaagtg gtgctgggaa atctcgccgc
     8401 ctcccctgtc gtcacgacgc tggaatatac cctgtttgat ggcgaacgcg tggtgcacag
     8461 cagcgccatt gatcatttgg caattgaaaa actgaccagc gccagctttg cttttactgt
     8521 cgaacagccg cagcaatggt cagcagaatc cccttatctt taccatctgg tcatgacgct
     8581 gaaagacgcc aacggcaacg ttctggaagt ggtgccacaa cgcgttggct tccgtgatat
     8641 caaagtgcgc gacggtctgt tctggatcaa taaccgttat gtgatgctgc acggcgtcaa
     8701 ccgtcacgac aacgatcatc gcaaaggccg cgccgttgga atggatcgcg tcgagaaaga
     8761 tctccagttg atgaagcagc acaatatcaa ctccgtgcgt accgctcact acccgaacga
     8821 tccgcgtttt tacgaactgt gtgatatcta cggcctgttt gtgatggcgg aaaccgacgt
     8881 cgaatcgcac ggctttgcta atgtcggcga tatcagccgt attaccgacg atccgcagtg
     8941 ggaaaaagtc tacgtcgagc gcattgttcg ccatatccac gcgcagaaaa accatccgtc
     9001 gatcatcatc tggtcgctgg gcaatgaatc cggctatggc tgtaacatcc gcgcgatgta
     9061 ccatgcggcg aaagcgctgg atgacacgcg actggtgcat tacgaagaag atcgcgatgc
     9121 tgaagtggtc gatattattt ccaccatgta cacccgcgtg ccgctgatga atgagtttgg
     9181 tgaatacccg catccgaagc cgcgcatcat ctgtgaatat gctcatgcga tggggaacgg
     9241 accgggcggg ctgacggagt accagaacgt cttctataag cacgattgca ttcagggtca
     9301 ttatgtctgg gagtggtgcg accacgggat ccaggcacag gacgaccacg gcaatgtctg
     9361 gtataaattc ggcggcgact acggcgacta tcccaacaac tataacttct gtcttgatgg
     9421 tttgatctat tccgatcaga cgccgggacc gggcctgaaa gagtacaaac aggttatcgc
     9481 gccggtaaaa atccacgcgc gggatctgac tcgcggcgag ttgaaagtcg aaaataaact
     9541 gtggtttacc acgcttgatg actacaccct gcacgcagag gtgcgcgccg aaggtgaaac
     9601 gctcgcgacg cagcagatta aactgcgcga cgttgcgccg aacagcgaag cccccttgca
     9661 gatcacgctg ccgcagctgg acgcccgcga agcgttcctc aacattacgg tgaccaaaga
     9721 ttcccgcacc cgctacagcg aagccgggca ttctatcgcc acttatcagt tcccgctgaa
     9781 ggaaaacacc gcgcagccag tgcctttcgc accaaataat gcgcgtccgc tgacgctgga
     9841 agacgatcgt ttgagctgca ccgttcgcgg ctacaacttc gcgatcacct tctcaaaaat
     9901 gagtggcaaa ccgacatcct ggcaggtaaa tggcgagtcg ctgctgaccc gcgagccaaa
     9961 gatcaacttc ttcaagccga tgatcgacaa ccacaagcag gagtacgaag ggttgtggca
    10021 gccgaatcat ttgcagatca tgcaggaaca tctgcgcgac tttgccgtag aacagagcga
    10081 tggtgaagtg ttgatcatca gccgcacagt tattgcaccg ccggtgtttg acttcgggat
    10141 gcgctgtacc tacatctggc gcatcgctgc cgatggccag gttaacgtgg cgctttccgg
    10201 cgagcgttac ggcgactatc cgcacatcat tccgtgcatc ggtttcacca tggggattaa
    10261 cggcgaatac gatcaggtgg cgtattacgg tcgtggaccg ggcgaaaact acgccgacag
    10321 ccagcaggct aacatcatcg atatctggcg cagcaccgtc gatgccatgt tcgagaacta
    10381 tcccttcccg cagaacaacg gcaaccgtca gcatgtccgc tggacggcac tgactaaccg
    10441 ccacggcaac ggtctgctgg tggttccgca gcgcccaatt aacttcagcg cctggcacta
    10501 tacccaggaa aacatccacg ctgcccagca ctgtaacgag ctgcagcgca gtgatgacat
    10561 caccctgaac ctcgaccacc agctacttgg cctcggctcc aactcctggg gcagcgaggt
    10621 gctggactcc tggcgcgtct ggttccgtga cttcagctac ggctttacgt tgctgccggt
    10681 ttctggcgga gaagctaccg cgcaaagtct ggcgtcgtat gagttcggcg cagggttctt
    10741 ttccacgaat ttgcacagcg agaataagca atgaggatca tcgataactt agaacagttc
    10801 cgccagattt acgcctctag caagaagtgg caacgctgcg ttgaagcgat tgaaaatatc
    10861 gacaacattc agcctggcgt cgcccactcc atcggtgact cattgactta ccgcgtggag
    10921 acagactccg cgaccgatgc gctatttacc gggcatcgac gctattttga agtgcattac
    10981 tacctgcaag ggcagcaaaa aattgaatat gcgccgaaag agacattaca ggtagtggaa
    11041 tattatcgtg atgaaactga ccgtgaatat ttaaaaggct gcggagaaac cgttgaggtc
    11101 cacgaagggc aaatcgttat ttgcgatatc catgaagcgt atcggtttat ctgcaataac
    11161 gcggtcaaaa aagtggttct caaagtcacc atcgaagatg gttatttcca taacaaataa
    11221 caactacggc ggcaaaagga gtttgccgcc accgctaccc tacacatttt cggagatgtg
    11281 ttatgtctga taccaaacgt aatacaatcg gcaaattcgg cttgctctcg ctgacttttg
    11341 ctgccgttta cagctttaac aacgttatca ataataatat tgagcttgga ctggcctcgg
    11401 caccgatgtt tttcctcgcg acgatttttt attttattcc cttctgtctg atcatcgcag
    11461 aatttgtttc gttaaataaa aactcagaag ccggtgtcta cgcgtgggta aaaagttcgc
    11521 tgggcggacg ttgggcattt attactgcct atacctactg gttcgtaaac ctgttctttt
    11581 tcacctcact gttgccgcgc gttattgctt atgcttcgta tgccttcctc ggatacgaat
    11641 atattatgac gccggttgcc accaccatta tcagtatggt gctgttcgcc ttctccacct
    11701 gggtttccac caacggggcg aaaatgttgg ggccaattac ctccgtcact tcaacgctga
    11761 tgctgctgtt aacgctctcc tacattttac tggcaggtac ggcgctggtt ggcggcgtac
    11821 agcctgctga ccccatcacc gttgacgcga tgatcccgaa cttcaactgg gcgttcctcg
    11881 gcgttaccac ctggatcttt atggccgcag gtggcgcgga gtccgtcgct gtgtacgtta
    11941 acgacgtcaa aggcggttcg aaatcgttcg ttaaagtgat catcctcgcc gggattttta
    12001 tcggcgtact gtattccgtc tcctcggtgc tgattaacgt cttcgtcagc agcaaagagt
    12061 tgaaatttac cggcggatcg gtgcaggtat tccatggcat ggcggcgtat tttggtctac
    12121 cggaagcgtt gatgaatcgc tttgtcggtc tggtgtcctt taccgcgatg ttcggttccc
    12181 tgctgatgtg gaccgcaacg ccggtgaaaa ttttcttctc cgaaatcccg gaaggcatct
    12241 ttggtaagaa aaccgtcgaa ctgaacgaaa acggcgttcc ggcgcgcgca gcgtggatcc
    12301 agttcctgat cgtcatcccg ctgatgatta tcccgatgct cggttccaat accgtgcagg
    12361 atctgatgaa tacaattatt aatatgaccg ccgcagcgtc catgcttccg ccgttattca
    12421 tcatgctggc ttacctgaat ttacgcgcca aattagatca cctgccacgc gatttccgta
    12481 tgggctcccg ccgcaccggt attatcgttg tttcaatgct gattgcgata tttgccgtag
    12541 ggtttgtcgc ttcgacattc ccgactggcg cgaatattct gaccatcatt ttttataacg
    12601 tcggc
//
LOCUS       DAJTYD010000110        12589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTYD010000110 DAJTYD010000000
VERSION     DAJTYD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.110"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            240..1337
                     /gene="mltA"
                     /locus_tag="OO585_002764"
     CDS             240..1337
                     /gene="mltA"
                     /locus_tag="OO585_002764"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6630294.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1414..2220
                     /gene="tcdA"
                     /locus_tag="OO585_002765"
     CDS             1414..2220
                     /gene="tcdA"
                     /locus_tag="OO585_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6630295.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2271..2714)
                     /gene="csdE"
                     /locus_tag="OO585_002766"
     CDS             complement(2271..2714)
                     /gene="csdE"
                     /locus_tag="OO585_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6630296.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2714..3919)
                     /gene="csdA"
                     /locus_tag="OO585_002767"
     CDS             complement(2714..3919)
                     /gene="csdA"
                     /locus_tag="OO585_002767"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6630297.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4111..4338
                     /locus_tag="OO585_002768"
     CDS             4111..4338
                     /locus_tag="OO585_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6630298.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4689..5606
                     /gene="gcvA"
                     /locus_tag="OO585_002769"
     CDS             4689..5606
                     /gene="gcvA"
                     /locus_tag="OO585_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6630299.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5625..6020
                     /locus_tag="OO585_002770"
     CDS             5625..6020
                     /locus_tag="OO585_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6630300.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6013..7113
                     /gene="rlmM"
                     /locus_tag="OO585_002771"
     CDS             6013..7113
                     /gene="rlmM"
                     /locus_tag="OO585_002771"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6630301.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7157..7888)
                     /gene="fucR"
                     /locus_tag="OO585_002772"
     CDS             complement(7157..7888)
                     /gene="fucR"
                     /locus_tag="OO585_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6630302.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7946..8368)
                     /gene="fucU"
                     /locus_tag="OO585_002773"
     CDS             complement(7946..8368)
                     /gene="fucU"
                     /locus_tag="OO585_002773"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6630303.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8370..9788)
                     /gene="fucK"
                     /locus_tag="OO585_002774"
     CDS             complement(8370..9788)
                     /gene="fucK"
                     /locus_tag="OO585_002774"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6630304.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILIVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9785..11530)
                     /gene="fucI"
                     /locus_tag="OO585_002775"
     CDS             complement(9785..11530)
                     /gene="fucI"
                     /locus_tag="OO585_002775"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6630305.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11563..>12589)
                     /gene="fucP"
                     /locus_tag="OO585_002776"
     CDS             complement(11563..>12589)
                     /gene="fucP"
                     /locus_tag="OO585_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6630306.1"
                     /translation="LFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPF
                     VTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSA
                     YKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARI
                     RHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTG
                     TWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGI
                     KNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARF
                     RSQTATN"
BASE COUNT         3012 a         3151 c         3335 g         3091 t
ORIGIN      
        1 ccgacgagct accaggctgc tccaccccgc gtccgtggat gcgcactata ctccgctcgc
       61 gttttcatgc aacctttttt ttaccccgat cacggattta gcatgatttt gaacaaaaac
      121 aaaccactaa agcctatttt ttgtgcaaaa tttgcgtcag ggagaggctg ctcatttcat
      181 taaacgcggc ggtttgttat cttcgttgcg ccttattttt taacctgaag aagagaacaa
      241 tgaaaggacg ttgggtaaag taccttctta tgggcacggt tgtggcaatg cttgccgcct
      301 gctcttccaa atcaaccgat cgcggacagc aatataaaga cgggaaattt acccagcctt
      361 tctctctggt gaaccagcca gatgccgttg gcgcgccgat taacgccggt gattttgccg
      421 agcaaattaa ccatatccgt aattcgtcac cgcgtctgta tggcaaccag agtaatgttt
      481 ataacgcggt gcaagagtgg ctgcgcgcag gcggtgatac ccgcaatatg cgccagttcg
      541 gcattgatgc ctggcagatg gaaggtgccg acaactatgg taacgtgcag tttacaggct
      601 attacacgcc ggtaattcag gcgcgccata cccgccaggg cgagttccag tatcctattt
      661 accgtatgcc gccaaaacgt ggtcgtctgc cgtctcgtgc ggagatctac gcgggggcat
      721 tgggcgataa atacatcctc gcttacagca actctctgat ggataacttc attatggatg
      781 tgcagggtag tgggtatatc gactttggtg atggcagtcc gcttaacttt ttcagctatg
      841 cagggaaaaa cggtcatgcc tatcgcagca ttggtaaggt gctgatcgac cgtggcgaag
      901 tgaaaaaaga agatatgtcg atgcaggcga ttcgtcattg gggcgaaaca cacagtgaag
      961 ccgaggttcg cgagctgctg gaacagaacc cgtctttcgt cttctttaaa ccgcaatctt
     1021 ttgcaccggt gaaaggggca agtgcggtgc cgctggttgg ccgtgcttct gttgcctccg
     1081 atcgttcaat tattccgcca ggtactacct tgctggcaga agtgccgctg ctggataata
     1141 acggcaaatt taacggtcag tacgaattgc gtctgatggt ggcgctggat gtcggtggcg
     1201 caatcaaagg ccaacacttc gatatctatc aagggatcgg gccggaagcc ggacatcgcg
     1261 caggttggta caaccactat ggacgtgtct gggtgctgaa aaccgccccg ggcgcaggta
     1321 acgtctttag cggctgatgt gttattctgt ataccgtttt tacggatacg acagggtgag
     1381 ggataacctc acccttttta attctgaggt gctatgtctg tggtaattag tgatgcatgg
     1441 cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca actgtttgct
     1501 gacgcgcata tttgtgtggt tggtatcggt ggtgtcggtt cctgggcggc ggaagcgctg
     1561 gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg atgatgtgtg cgtcaccaat
     1621 accaatcggc aaattcacgc tctgcgcgat aacgtcgggc tggcaaaagc ggaagtcatg
     1681 gcggagcgca ttcgccagat taacccggag tgccgtgtaa cggtggtgga tgatttcgtg
     1741 acgccggata acgtagcgca atatatgagt gccggttatt cgtacgtgat tgatgccatt
     1801 gatagtgtac gacccaaagc ggcgctgatt gcttattgtc ggcgcaataa aatcccgctg
     1861 gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc agattcaggt taccgatctg
     1921 gcgaaaacga ttcaggaccc gctggcggcg aagttgcgcg agcgcctgaa aagcgatttt
     1981 ggcgtagtga aaaacagtat aggtaagctc ggcgtggatt gcgtgttttc tactgaagcg
     2041 ctggtgtacc cgcagtcaga cggtacggtg tgtgcgatga aagccacggc agaagggccg
     2101 aagcggatgg attgtgcatc tggatttggc gcggcaacga tggtgaccgc cacctttggg
     2161 tttgttgcgg tttctcatgc gctgaagaag atgatggcga aagcagcgcg tcagggttaa
     2221 acaatcaggt cggatgcgac gcgaacgtct tatccgacct ggttctgtgc ttaaacctgc
     2281 ttcgccgcag cgataattgc ctcgcttaac gcattcaacc cctggctgcg tgaggcgcta
     2341 agctgcccac gcaatcccag ctcatcaaac aatgccagcg gcgactgtgc ctgtaattcg
     2401 gcggcggttt tcccctcaac ggcagtcaac aacaccgcca acaggccgag cacaatgcgg
     2461 ccttcgctgt cgccaaagaa gtgcatttta ccgttttcag ccacggtata tcccagccag
     2521 acgcggtttt cgcatccggc aatctcttta gcctgcgctt ttaactcgtc tggcaaagcc
     2581 ggaagctgtt tccccagcat gatcaactgg cgatatttat cttcccattg cgtcaacggt
     2641 gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg tccggcgaat
     2701 tgcgggtttg tcattaatcc accaataatt ccagcgcgcg gtcaacggca ttcaccagcg
     2761 catccacatc actctttgta ttatatggcg caaaagaggc gcgcagtgtg ccggttacgc
     2821 ctaattctgc cagtagcggc tgagcacaat gttgcccggc ccgcaaggca ataccgtact
     2881 ccgccagtag cgtcaccata tcgctgtgat gaacgccagc aaaatcaaag gccagcaggc
     2941 tggaatcctg gcagcggaat gaacgaaagc ctggacgttt cgccagcgca tcttccgcca
     3001 gcgttgctaa gctatggctc cagttttcgg cctggttgat atcgtaatct gccagccatt
     3061 ccagcgccgc gcttaatcct atgacaccag ccacatttgg cgttcctgct tccagtttcc
     3121 acggcgcaga ttgagtcgtg aagccgtcaa aactcacttc gtgaatcatt ttgccgccac
     3181 ccagccaggg cgacatcgct tccagcagtt ctgatttgcc atacagcacg ccgatacccg
     3241 tcgggccata cagtttgtga ccagagaaag catagaaatc gatatccagt tgctgaacat
     3301 ccgcggggaa atgcaccgcc ccctgagcac catcaaccat caccaccatc cctgctgaat
     3361 gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc agtaacgttc gacatctgac
     3421 tcaacgccag aatccgacta cggggagtaa tcagttctgg caacaaatcg acatccggca
     3481 gtcgctgcgc attaagcggc aatttcacca ctttggctcc agtttgttgg gcgaccatca
     3541 gccaggggac gaggttggcg tggtgttctg ccacgctgac aataatctca tcgcccggtt
     3601 gcagacgcgg acgcgcatag cattgtgcca ccatgttgat ggattcagtg gtgccgcgcg
     3661 tccagacgat agttttatca tccggtgcat tcagtaattg cgccactttc tctcgtgcag
     3721 cttcataacg cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg acgtttccgg
     3781 cgctcaggct gtaaaactgt cgagtggctt caaccacggc ttcaggttta agcgcggttg
     3841 cggcgctgtc gagatagacg cccgcatcct gtagtgcggg aaactgggcg cgaaactgcg
     3901 cgggattaaa aacgttcatg gtactcctcg gcttgatgca ccgatcgtga cgcaaaattc
     3961 actttgatac aaggagagtg ctaaccatca gaattatctg gatatcctgg cgaagccgcg
     4021 taagtaacat atgctgaata gtgttaggcg ttaattttag cctgttaatc aatactatta
     4081 agatttatta atcgctttaa ggagaagatg atgaaaaaga ctgccgcaat tatttctgcc
     4141 tgtatgctga cttttgccct gagcgcctgt tccggttcga actatgtgat gcacaccaat
     4201 gacggacgta ccatcgtctc tgacggcaaa ccacagactg ataacgatac cggtatgatt
     4261 tcgtataaag acgctaatgg caacaaacag cagatcaacc gtactgacgt gaaagagatg
     4321 gtcgaactgg atcagtaatt cgcgatcgca aggtaaaaaa aagcaccgca attaggcggt
     4381 gctacattaa tcactatgga cagacagggt aaatgtacag gaagtgaaaa aaggtagctt
     4441 tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa caacacaaca tcacaaccgt
     4501 aagccaaaag ttcaccagaa cacgcattcc gataaaactt ttcgttccgg ctcaggaagt
     4561 gccgccacta taggtatttg ctggtagaag ctcaacggac aatttataat ggctcagatt
     4621 aaaaaaacta ataggttaca tagtgtgatc taattgttaa attcatttaa catcaaagtt
     4681 taatagccat gtctaaacga ttaccaccgc taaatgcctt acgagttttt gatgccgcag
     4741 cacgccattt aagtttcact cgcgcagcag aagagctttt tgtgacccag gccgcagtaa
     4801 gtcatcaaat caagtctctt gaggattttt tggggctaaa actgttccgc cgccgtaatc
     4861 gttcactcct gctgaccgag gaagggcaaa gctatttcct cgatatcaaa gagatatttt
     4921 cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg gcgttgacgg
     4981 tcagtttact ccccagtttc gccattcatt ggttggttcc gcgactttcc agctttaatt
     5041 cagcttatcc gggaattgac gttcgaatcc aggcggttga tcgtcaggaa gataagctgg
     5101 cggatgatgt tgatgtggcg atattttatg gtcggggcaa ctggccgggg ctacgggtgg
     5161 aaaaactgta cgccgaatat ttattgccgg tgtgttcgcc gctactgctg actggcgaaa
     5221 aacccttgaa gacaccggaa gatctggcta aacatacgtt attacatgat gcttcgcgcc
     5281 gtgactggca gacatatacc cgacagttgg ggttaaatca tatcaacgtt cagcaagggc
     5341 caatttttag ccatagcgcc atggtgctgc aagcggctat ccacgggcag ggagtggcgc
     5401 tggcaaataa cgtgatggcg caatctgaaa tcgaggccgg acgtcttgtt tgcccgttta
     5461 atgatgttct ggtcagtaaa aacgcttttt atctggtttg tcatgacagc caggcagaac
     5521 tgggtaaaat agccgccttt cgccaatgga tcctggcgaa agccgctgct gaacaagaaa
     5581 aattccgctt tcgttatgaa caataattta cgtagggtac gaccatgacc agccgtttta
     5641 tgctgatttt cgccgccatt agcggcttca tttttgtggc tctgggcgct tttggcgcgc
     5701 atgtgttaag taaaaccatg ggagccgttg agatgggctg gatccagacc ggcctcgaat
     5761 accaggcgtt tcatacgctg gcgatcttag gtctggcggt ggcaatgcag cgtcgcatca
     5821 gtatctggtt ttactggagt agcgttttcc tcgcgttagg cacggtgttg ttcagcggca
     5881 gcctttattg cctggcgctg tcccatctgc gtttgtgggc gtttgtcact ccggttggcg
     5941 gcgtgagctt cctcgcgggc tgggcgttaa tgttagttgg tgctatccgt ttaaagcgca
     6001 agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc cgggctttga aaaagagtgc
     6061 gccgcagaaa ttaccgataa agccggccag cgggaaattt tcggttttgc ccgcgtgaaa
     6121 gagaattcgg gttatgtcat ttatgaatgt tatcaacctg atgatggcga taagttaatc
     6181 cgtgagctgc cgttcagttc attaattttt gcccgccagt ggtttgtggt gggggaactc
     6241 ctgcagcatt tgccgccaga agatcgtatt acccccattg tcggcatgtt acagggcgta
     6301 gtagagaagg gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag caaagagttg
     6361 ctgaaattct gccgtaaatt tactgttccg ctacgcgctg ccttgcgcga tgccggggtg
     6421 ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg tattctttat tgcaccaggc
     6481 tgctgctata ccggttactc atacagcaac aataattcgc cgttctatat gggcattccg
     6541 cgcctgaaat ttccggcaga ggcgccgagt cgttccacgc tcaaactgga agaggcattt
     6601 catgtgttta ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat gtgggcggtg
     6661 gatttaggcg cttgccctgg cggctggacc taccaactgg tgaagcgcaa catgtgggtt
     6721 tattccgtcg acaacggccc gatggcgcaa agtctgatgg ataccgggca ggtgacgtgg
     6781 ctgcgggaag acggtttcaa attccgtccg acacgcagca atatctcctg gatggtatgc
     6841 gatatggttg aaaaaccggc taaagttgcg gcattgatgg cgcagtggct ggttaatggc
     6901 tggtgccgtg aaaccatttt caatctcaaa ctgccgatga aaaaacgcta cgaagaagta
     6961 tcacacaatc tggcgtatat tcaggcacag cttgatgaac atggcataaa tgctcagatt
     7021 caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg ccgcatctgg
     7081 gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg tcgtcttcgg
     7141 cctgctaccc tttccgtcag gctgttacca aagaagttgc aacctggcgc tcatcagaaa
     7201 taatgtgcgt tacctcatcc agatgcccga tgcgggcttc ccctgaacga ttaaatttac
     7261 ttttatcaat caataacaac gattgcgcgg cacgttttaa tagcatcgat ttgtaatcag
     7321 cgttgatcgc attggagtcc cacagtgcgc cgctgctatc gatcccttca caagaaaaaa
     7381 taaacagatc gatttccagc gatttaagtt gggaaatcag cgagggattg acgtaacagc
     7441 catatttgcg ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg cgtttaccga
     7501 gttcatagca aatcggatgg ctattggtga agacctgaat gttgatgtca ggcaactggc
     7561 gtgccagata ccagcaagtt gaactggcgt ctaaggctat caccatccct tcttcaatcc
     7621 acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata atggcttttc agcctgatgt
     7681 gaaagggatc gccactgtct tgattttgac ggtggatata tttagcgcgt ccatgattgc
     7741 gcagaatttt accctgcgtc tgtaattcat tgagatcgcg acgaatggtt tctttactga
     7801 cctttagctg ttcagagaga gcttccgtgg tcaggctggt atggttcagc agcaggtcga
     7861 ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc atcattcgat aaccgcccgc
     7921 gtcatgcggg cgtaccggta tgagattacg gtgttacccc tttttttaaa agaatattcc
     7981 cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc cgttcataaa
     8041 acgcaaaacg attgatgcgg atgatgtcag gacacggggc ttgtagtgaa agcgcattac
     8101 ggtaacgtcg ttctacttca ggatcgagag tgtcaccttc taccgccgcc atcatcacca
     8161 gcggcggtgc ataactgtcc agttcaaata acgggataat cgcctggagc aagtcgctca
     8221 ccaacaggcc atcagcgcgg atcacctgcg gtcccatcga atgggcggga aagtgagcat
     8281 cggaaaaaat aatttcatct ccatgtccca tctctgccag cactttcaat agttcgggag
     8341 aaattaacgg cgaaattgtt ttcagcattt cacacttcct ctataaattc aggttcagtt
     8401 tgcgggtaga aataacggta ctgataatga atctgtgcgc gggcttcttc agggctgtta
     8461 aattccccta cgccatacca accgaacagc gcagctcctg cgacggtcgt ttcggcgtcg
     8521 tcgagaactt ttaccggaat atcaagcata ttggctttaa tctgattcca caatgtgttg
     8581 cgacttcctc caccgactaa caataattca gaggccttaa agtgcccgat tttttccagc
     8641 atctgtagat tgcgctgtaa ttgcgcagtt aacccttcca gcgccgcgcg atagaaatgc
     8701 ccccgcgtgg tattaagcgt cactccttgc cagccagcgt tctgacacga caataaatca
     8761 cactgcattt ttacgccatc cgcgctaggc gcgataagac gagcttcttc aatcaacatt
     8821 tgccagggtg tttcagccgt ccagaacagt tttctcaccc attccagcac gccggatgcc
     8881 agccattgca tacctgggtt atacaaccct gcctggctat ccagttcgca ggtggaaccg
     8941 gcgtactgac ttaacagcga agtatcaacc tgggcgctac ggactattaa aatttcccat
     9001 gtaccggaag agagcacggg ttcattttgg tcggcaccag cgccaaaaag ggcgaactgg
     9061 gtatcgtgac ctgcggaaat caccggtatg ccaacgggta agccgagcat tgctgcggcg
     9121 ctgttctgta gcgtaccaat ctgttcaccc gcttccacca gacgagggaa gagtcggcgt
     9181 ggaataccag tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat atccagcatc
     9241 tggctggttc cggccatcgt gatatcagta gtgaattcgc cggttaaacg gtggttaatc
     9301 agcgacgaaa taaagaacca ggcgtgcgtg cgttccagca gttgtggatg attttctttc
     9361 aaccacacca acttatataa cgtattgaaa ctaaaggctc cgacgccaga aatagcctgc
     9421 aaccgctgtg cggagatcaa ctgttcaata ttgtccataa ccgctgctgt tcgcggacat
     9481 ttccagctaa tgatcggata gagcagattg ccttgcttat ctaccagagc gccatccaca
     9541 ccaaaggtgg tgacggcgat accgcggatg tggcattcag tcagttcact attgatttgc
     9601 cgacagcaat cagcaaagcg ttgcaaaatg gcgtctaaag accactggtg ccaggtgttg
     9661 ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat ttttccctgc
     9721 cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag gataacttct
     9781 tgtttcataa tcgctcctgc aatacttcaa tatccatgcc gtgcgcagcc caggcagaag
     9841 gacgatacac tttggtctct tcaacgttgt gcatacatac cgggatacgc agcatggagg
     9901 cgagagtgat aaagtcagcg ccaacgtggc cgatggtcag aaccccatgg ttagcgcccc
     9961 agttcgccat taccgagtac acatccgtaa acggcccttt accggtgagg cgcggtgcaa
    10021 accaggtggt tggccaggtt gagttggtgc gtttgttgag gatgtcatgc acatccttcg
    10081 gcaattccac gctccagcct tccgcgattt gcagtaccgg tcccaggcct ttgatgatgt
    10141 tgacacgagt catggtgaac gggacgccgc cttcggtaag gaagcgggaa gagtaaccgc
    10201 cgccacggaa gtattcgtgg atcgccgggc accattcggt agcggcgagg caagcgtcag
    10261 cctcttgctg agagatttcc cagtgcggct tcatcgtcgg gttaccttcg ctgtcgcgtt
    10321 gtttacagga accgtccagc gcagcagaac ctgagttgat caaatggatg atgccgtgtt
    10381 ctgccagtcc atcgagttta tgccccgtta cacgctcaat tgcttctggt gaccagtagg
    10441 tacgcacatc ggcaaatacc tgagcggtgc cggtgagctg gtgacccatt agcattgcca
    10501 cgccgttaag actgtcgttt tcggtcgcca cgacaaaggg ttcgcgcacg ccattccagt
    10561 caaatgaact gttgaggatc gcttcggcgg tgtcaccatt gggatattga tcggtccagt
    10621 gacgttgccc ctggaagccc gcagcgatgg cgttgtagcc aagtgattct tccacgcgac
    10681 caatatcggc cagtttgctg ttgccttgca tcatgtcgcg gatacacatc gccatcagta
    10741 aactttcgcg cagaactgcg cggctttgct cggcattgcg ttgatactgt ttgttatttt
    10801 catcttcgcc atagcggaag tttttatcag cccaggccag tgccatttcc agttcggctt
    10861 cgtcgtaaat cttctgatcg atacggcgac gcagttcggt catatccacc gcctggactt
    10921 tcattcccag ccaggattca aagaagttgt gatcaacgat ggaaccggcg atacccatcg
    10981 aaacgccgcc cagcgacaga tagcttttac ctttcatgct ggcgacggcc aaaccggcgc
    11041 gggcaaagcg cagcagtttt tcttcaacat cggcaggaat cgatgtgtca tcggcatcct
    11101 gaacgtcatg accgtaaatg gagaatgccg ggatgccttt ctggctgtga gctgccagag
    11161 ccgctgccag gtaaacagcg ccgggacgtt cagtgccgtt aaagccccaa atggctttcg
    11221 ggcgggttgg atccatgtcg atggtttcac tgccatagca ccagcaaggc gttaccgtaa
    11281 tggtgaggcc tacattctgg ctgctgaatt tttcttcgca agcagcggct tcagccatac
    11341 ccgcgataca ggtatcggaa atgacacact cgacggcagc tccgcaggca tggcgcagtt
    11401 tctcggtcag cagtgcggcc gtagctttcg ccatattcat tgtttgttct tcaagcgact
    11461 cacgaacacc catgcgacga ccgtcaataa ccgggcggat accaattttc ggtaagctga
    11521 tttttttcat tacagattcc tcactttatt cggaaaatat gttcagttag ttgccgtttg
    11581 agagcggaaa cgggcaaaga taaagatgac cgcgaagcag agtgcgggga tcagttcagc
    11641 agtggggatg ttgcccgccg cgtcactgac aaaacccatg accggagtga caataccgcc
    11701 gccaataatg gtcataacga tgaaggacga accgtatttg gtgtcctggc cgagattctt
    11761 aatgcccagc gagaagattg ttgggtactg aatcgacata aaggcgctgc ataaagtcag
    11821 ggctattaag cccacatgac cgccagcgaa ggctgagatc aggcacagtg ccatagcgat
    11881 taatgcgtag gcggccagga ctttgtgtgg tgcgaagcga ctgatgagcc aggtaccggt
    11941 gaaacgacca ataaagaagc acaccatggt tccggttaaa tagttagcgg caaagcctgc
    12001 agtcatacct ggaatttctt ctacagcgta gcgaatcaaa tagctccagc aggccgtttg
    12061 tgcgccaacg tagcagaatt gcgctaatac tgcccagcgc cagtggcgaa tacgcgccag
    12121 gcgagaaagc gatgcggaga acgatccttg tttggcgtca ctgtgattat cactctgcaa
    12181 tgccgggaat ttcgtcagca tgatcagcag ggcgaccagt aacacgatag ccacgatgat
    12241 catataaggt gtctgtaccg ataataccag gctgtgttta tacgcactca attgctctgg
    12301 agacatttta tcgagaacgt cttgcgattg atgtggcacg ttagacaaaa taagactttg
    12361 cccaaagaca accgcgataa ttgcgccaaa cgagttaaat gtttgcgcaa gatttaagcg
    12421 gaagtgacca ctgctttccg gccctaatac cgtaacaaaa gggtttgcgg cagtttccag
    12481 acaacctaat ccggctgcaa taataaatag gccaactaaa aataaggtgt agttcattat
    12541 ttctgcagcg ggccagaata atgcagcgcc cagggcatat aaaaataac
//
LOCUS       DAJTYD010000111        12468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTYD010000111 DAJTYD010000000
VERSION     DAJTYD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.111"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(29..1369)
                     /gene="fadL"
                     /locus_tag="OO585_002777"
     CDS             complement(29..1369)
                     /gene="fadL"
                     /locus_tag="OO585_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6630307.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1741..2025
                     /locus_tag="OO585_002778"
     CDS             1741..2025
                     /locus_tag="OO585_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6630308.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2205..3515
                     /gene="fadI"
                     /locus_tag="OO585_002779"
     CDS             2205..3515
                     /gene="fadI"
                     /locus_tag="OO585_002779"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6630309.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3515..5659
                     /gene="fadJ"
                     /locus_tag="OO585_002780"
     CDS             3515..5659
                     /gene="fadJ"
                     /locus_tag="OO585_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6630310.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            5862..6347
                     /gene="sixA"
                     /locus_tag="OO585_002781"
     CDS             5862..6347
                     /gene="sixA"
                     /locus_tag="OO585_002781"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6630311.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7022..7282
                     /locus_tag="OO585_002782"
     CDS             7022..7282
                     /locus_tag="OO585_002782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6630312.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7289..7585
                     /locus_tag="OO585_002783"
     CDS             7289..7585
                     /locus_tag="OO585_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6630313.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7667..10309
                     /gene="yfcU"
                     /locus_tag="OO585_002784"
     CDS             7667..10309
                     /gene="yfcU"
                     /locus_tag="OO585_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6630314.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10329..11081
                     /locus_tag="OO585_002785"
     CDS             10329..11081
                     /locus_tag="OO585_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6630315.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            11097..11606
                     /locus_tag="OO585_002786"
     CDS             11097..11606
                     /locus_tag="OO585_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6630316.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11603..12091
                     /locus_tag="OO585_002787"
     CDS             11603..12091
                     /locus_tag="OO585_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6630317.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            12088..>12468
                     /locus_tag="OO585_002788"
     CDS             12088..>12468
                     /locus_tag="OO585_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6630318.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDG"
BASE COUNT         3127 a         2988 c         3317 g         3036 t
ORIGIN      
        1 aggtgacttt atccaggcga acgcgttatc agaacgcgta gttaaagttc gtaccgaaca
       61 gccaggcttt accttcagac tcgaactggt atgggccttc gttaattttc acgctctgac
      121 cgtgcatata agaaacacca acgtcgactg aagcatcttt attaaatgcg taagtcgtac
      181 ctgcactcag ccagaaacgg tcctggtccg gaatggagat agaacgattc tgagccggaa
      241 ctgggctgtc atcaaaggcg ataccggtac ggaaggtcca gttatcatcg tagtaataag
      301 tggtacccaa cgcgatgcgg taagcatctt taaagccttc atgtttctgg aacagcgtgt
      361 tgccactggt tgaggtcgct ttcagctgct ggaactgact ccagctggtg taagccaggc
      421 tatagtgaat cgcccactgt ggatcaacac ggttataacc tgacacttcc cacatttcag
      481 gcaggttcag cgtcagataa cccgattgcg ttgcgccacc tgtcgcggta ggaattggca
      541 aaccgtagtt attaaacgca cgattaagat cgctgctgta gttacctttg aagtcaattt
      601 tcacttcaga acggtaggtc agtgcatagc ggttattttt atccagttca tacaggattc
      661 cggcgttcca gccaaagccc cactggttac cgttcagatg agcgattttg gtattactgt
      721 cgataccgtt ggcggtagct gccaatgctt gcccttgagg agtttgcccg gcaggagatt
      781 gcataatctg accagcaacc aactgcccca gatcgcctgc gaaacgttca attttcgcgc
      841 gagcgtagac ggcgttgaaa ccaagaccaa agctccatgc attatttaag cgatacgcac
      901 cgcttaagtt caggttcatg gtttcaaggt cagttgtacc cccgacagag ccgcctgcat
      961 aagtatcatt aaactctgtt gccagaccat agttagaggt aatagaagcg ccccaaccaa
     1021 attggtcgtt aatcggtgca acaaagtgca tgttcggaac ccatgccgta ggcgcgatgt
     1081 tatcggcttt caggctacga ccagacggag acgttccgct gatattcaca tccgggtcaa
     1141 tataaaccgc acccgcagaa aatgtcgggc ggtcaaacat ggtaatcaat gcggggttac
     1201 ggctaacgtt acctgcatca tcggcaattg cgccttcccc tgaataagcc cggcccaggc
     1261 cagaggaaga aaattcgttt aactgaaagc ctgccgacca ggcctgggtg gagataagtg
     1321 ccactgcgac tgcgagagca gactttgtaa acagggtttt ctggctcatg accataacct
     1381 caatgattta tttttataca aactatgtta cgtgctgtaa caggagcgcg aagtgtaggg
     1441 tctgaggtac atcttagaaa tcagaccagt ggcgagagta taggtcggac cagctggaat
     1501 gttgcaagta tgtttccatc ttttttcaaa tatgatctaa gaaacgcgat ccagctggca
     1561 aaatttggag gttgcgcact cgcggattcg ggtgaatttt gttttagatc atttttaagt
     1621 gtgatttcgg tcacttatcc gattttgcag aattaacaac tggagcagca ctttccggaa
     1681 gcgtaaaata tccacaacgt ttatctggca ccttgggtgc acatacagag gaaatcaacg
     1741 atgagtaaat gtagtgctga tgaaaccccg gtttgctgct gtatggatgt tggcaccatt
     1801 atggacaact ccgactgcac cgcgtcttac agccgcgtgt ttgcaaaccg cgcagaagca
     1861 gaacaaacgc tggcggcgtt gactgaaaaa gcccgtagcg tggaatccga accgtgcaaa
     1921 atctccccga ctttcactga agagtctgac ggtgtgcgcc tggatatcga tttcactttt
     1981 gcctgcgaag ctgaaatgct gatcttccag ctgggtctgc gttaattcct ctcgggtacg
     2041 ttcgcccctg tcagttgctg gcaggggcgt tttcttttct ttctcctgtc ttcactgtgt
     2101 catagctccc actttccttt ttctccgctt ggctaaatgt aaaaaaatgg ttaagactat
     2161 gatcaggtca gaccacttta tttatttttt tacaggggag tgttatgggt caggttttac
     2221 cgctggttac ccgccagggc gatcgtatcg ccattgttag cggtttacgt acgccttttg
     2281 cccgccaggc gacggctttt catggcattc ccgcggttga tttagggaag atggtggtag
     2341 gcgaactgct ggcacgcagc gagatccccg ctgaagtgat tgaacagctg gtctttggtc
     2401 aggtcgtaca aatgcctgaa gcccccaaca ttgcgcgtga aattgttctc ggtaccggaa
     2461 tgaatgtgca taccgatgct tacagcgtca gccgcgcttg cgctaccagt ttccaggcgg
     2521 ttgcaaacgt cgcagaaagc ctgatggcgg gaactatccg agcggggatt gccggtgggg
     2581 cagattcctc ttccgtattg ccaattggcg tcagtaaaaa actggcgcgc gtgctggttg
     2641 atgtcaacaa agctcgaacc atgagccagc gcctgaaact cttctctcgc ctgcgtttgc
     2701 gcgatttaat gcccgtaccg cctgcagtag cagaatattc taccggcttg cggatgggtg
     2761 acaccgcaga gcaaatggcg aaaacctacg gcatcacccg agaacagcaa gatgcattag
     2821 cgcaccgttc gcatcagcgt gccgctcagg catggtcaga cggaaaactc aaagaagagg
     2881 tgatgactgc ctttatccct ccttataaac aaccgcttgt cgaagacaac aatattcgcg
     2941 gtaattcctc gcttgccgat tacgcaaagc tgcgcccggc gtttgatcgc aaacacggaa
     3001 cggtaacggc ggcaaacagt acgccgctga ccgatggcgc ggcagcggtg atcctgatga
     3061 ctgaatcccg ggcgaaagaa ttagggctgg tgccgctggg gtatctgcgc agctacgcat
     3121 ttactgcgat tgatgtctgg caggacatgt tgttcggtcc ggcctggtca acaccgctgg
     3181 cgctggaacg tgccggtttg acaatgagcg atctgacatt gatcgatatg cacgaagcct
     3241 ttgcagctca gacactggcg aatattcagt tgctgggtag tgaacgtttt gcttgtgaag
     3301 tactggggcg tgcacatgcc actggcgaag tggacgatag caaatttaac gtgcttggcg
     3361 gttcgattgc ttacgggcat cccttcgcgg cgaccggcgc gtggatgatt acccagacat
     3421 tgcatgaact tcgccgtcgc ggcggtggat ttggtttagt taccgcctgt gctgccggtg
     3481 ggcttggcgc ggcaatggtt ctggaggcgg aataatggaa atggcatcag cgtttactct
     3541 taatgttcgt ctggacaaca ttgctatcat caccatcgac gtaccggatg agaaaatgaa
     3601 taccctgaag gcggaatttg cctcgcaggt gcgcgccatt attaagcaac tccgtgaaaa
     3661 caaagagttg cgcggcgtgg tgtttatctc cgcgaaaccg gacaatttta tcgccggagc
     3721 agacatcaac atgatcggca actgcaaaac ggtgcaagaa gcggaagctc tggcgcggca
     3781 gggccaacag ttgatggcgg agattcatgc tttgcccatt ccggttatcg cggctattca
     3841 tggcgcttgc ctgggtggtg ggctggagtt ggcgctggcg tgccacggtc gcgtttgtac
     3901 tgacgatcct aaaacggtgc tcggtttgcc tgaagtacaa cttggattgt tacccggttc
     3961 aggcggcacc cagcgtttac cgcgtctgat aggcgtcagc acaacattag agatgatcct
     4021 caccggaaaa caacttcggg cgaaacaggc attaaagctg gggctggtgg atgacgttgt
     4081 tccgcactcc attttgctgg aagtcgctgt tgagctggcg aagaaggatc gcccatcttc
     4141 ccgccctcta cctgtacgcg agcgtattct ggcggggccg ttaggtcgtg cgctgctgtt
     4201 caaaatggtc ggcaagaaaa cagaacacaa aactcaaggc aattatccgg cgacagaacg
     4261 catcctggag gttgttgaaa cgggattagc gcagggcacc agcagcggtt atgacgccga
     4321 agctcgggcg tttggcgaac tggcgatgac gccacaatcg caggcgctgc gtagtatctt
     4381 ttttgccagt acggacgtga agaaagatcc cggtagtgat gcaccgcctg cgccattaaa
     4441 cagcgtggga attttaggtg gtggcttgat gggcggcggt attgcttatg tcactgcttg
     4501 taaagcgggg cttccggtca gaattaaaga tatcaacccg cagggcataa atcatgcgct
     4561 gaagtacagt tgggatcagc tggagggcaa agttcgccgt cgtcatctca aagccagcga
     4621 acgtgacaaa cagctggcat taatctccgg aacgacggac tatcgcggct ttgcccatcg
     4681 cgatctgatt attgaagcgg tgtttgaaaa tctcgaattg aaacaacaga tggtggcgga
     4741 agttgagcaa aattgcgccg ctcataccat ctttgcttcg aatacgtcat ctttaccgat
     4801 tggtgatatc gccgctcacg ccacgcgacc tgagcaagtt atcggcctgc atttcttcag
     4861 tccggtggaa aaaatgccgc tggtggagat tattcctcat gcgggtacat cagcgcaaac
     4921 catcgccact acggtaaaac tggcgaaaaa acagggcaaa acgcccattg tcgtgcgtga
     4981 caaagccggg ttttacgtca atcgcatctt agcgccttac attaatgaag ctatccgcat
     5041 gctgaccaaa ggtgaacggg tagagcacat tgatgccgcg ctagtgaaat ttggttttcc
     5101 ggtaggtcca atccaacttt tggatgaggt aggaatcgac accgggacta aaattattcc
     5161 tgtactggaa gccgcttatg gagaacgttt tagcgcgcct gcaaatgttg tttcttcaat
     5221 tttgaacgac gatcgcaaag gcagaaaaaa tggtcggggt ttctatcttt atggtcagaa
     5281 agggcgtaaa agcaaaaaac aggtcgatcc cgccatttac ccgctcattg gcgcacaagg
     5341 gcaggggcga ctctccgcac cgcaggttgc tgaacggtgt gtgatgttga tgctgaatga
     5401 agcagtacgt tgtgttgatg agcaggttat ccgtagcgtg cgtgacgggg atattggtgc
     5461 ggtatttggc attggttttc cgccatttct cggtggaccg ttccgctata tcgattctct
     5521 cggcgcgggc gaagtggttg caataatgca acgactggcc acgcagtatg gttcccgttt
     5581 taccccttgc gagcgtttgg tcgagatggg cgcgcgtggg gaaagttttt ggaaaacaac
     5641 tgcaactgac ctgcaataag aaggtcaaag ctatatgaat ccgcgctgaa tggcggagtg
     5701 ttggtcaaaa tgtaaacgca tattgactat acttacgcca ttgaggtaaa aaacagcgtt
     5761 tcattcggtg aatggataag gcacaatgcc ggccaccgtt ttctttctct ggtttcagat
     5821 gaaagaaaat gggcgaatct ggttaacaaa agcggtgcaa tatgcaagtt tttatcatgc
     5881 gtcacggcga cgcagccctc gatgccgcca gtgattcggt tcgtcctctg accactaatg
     5941 gttgtgacga atctcgcctg atggcgaact ggctgaaagg tcaaaaagtg gaaatcgaac
     6001 gcgttctggt gagcccgttc ctgcgagccg agcaaacact ggaagaagta ggggattgtc
     6061 tgaacctgcc ttccagtgca gaagttctgc cggagttaac gccctgcggc gatgttggtc
     6121 tggtcagtgc ctatttgcag gcgttgacca atgaaggtgt tgcttcggta ctggtgatct
     6181 ctcacttacc gttagtcggg tatctggttg ccgagttatg tccgggcgaa acgccgccta
     6241 tgtttaccac ctcggccatt gccagcgtaa cgcttgatga aagcggaaac ggtacattca
     6301 actggcagat gagcccgtgt aatctgaaga tggcaaaagc tatctgatgt gttccaaatt
     6361 atgcccaatg aggtagattc attgggcata attgctcatc aggcagcgga tatctgtcaa
     6421 cctcactact atcgaagact ctccttatct ataaataaaa cactttctac attcattcgg
     6481 aaattattct tctgcacgta tcttgatttt gcccaaagtt acatgttgaa atgaataatg
     6541 tctgtttgac actcattgtc atttattaat acagataata gagttgaata atcaggcagg
     6601 atgtaattga aacttgatta aattctggct atagtgttta tatattatat tgtcaaaagt
     6661 ggataaatgc caatcgcatt atatattcat tgtttgtttt ttaagctgtt gatattcatt
     6721 attgatatac tttctatttt ttctaaatac ttattccgta ttttttaagg atgtccttaa
     6781 gataatcttt cttctgtcaa tatattaatt aaatctacat ttactcttta gtaaaattaa
     6841 attgcatcag gggtgtggaa acatcactat gcggccccac accaacgaat acaattttct
     6901 acgacctgtt ctttttggat cgcatgccgt attcaatggc tgggtataaa cattggctat
     6961 tgactgaatc aatgtattgc aatgtttata tttttaatta tttatagttt aaggacaaat
     7021 aatgagtaag tttgttaaaa cagctattgc tgcggcaatg gtgatgggcg tgttcacctc
     7081 tacggcaaca atcgctgcgg gtaacaacgg tactgcacgt ttttacggca ccattgaaga
     7141 ctcagtgtgt tctatcgttc cggacgatca caaactggaa gttgatatgg gtgatatcgg
     7201 tgccgaaaaa ctgaaaaata acggcaccac cacgccgaaa agtttccaga ttcgtctgca
     7261 agattgcgta tttgatactt aggaaacaat gaccactacc tttactggta ccgtttcttc
     7321 tgcaaatagc ggcaattatt acaccatttt caataccgat accggtgcgg catttaacaa
     7381 tgttagcctg gcgatcggtg actctctggg tacctcttac aaaagcggca tgggtattga
     7441 ccagaaaata gtgaaagaca cttctactaa taaaggcaaa gcgaagcaga cactgaactt
     7501 taaagcctgg ctggtcggcg cagctgatgc gccagatctg ggtaatttcg aagcaaatac
     7561 caccttccag attacttacc tgtaaggcat acccccatag cgacactgct atgggggatt
     7621 taaatggatt ttgaccgaac aagaataaaa acggatagaa acgtgtatgc ctgaccattc
     7681 tctttttcga ttacggattc ttccgtggtg cattgcgctg gcaatgtcag ggagttatag
     7741 cagtgtctgg gctgaagacg acattcagtt tgattcccgt tttctggaat taaaaggcga
     7801 cacaaaaatt gatctgaagc gtttttccag ccagggatat gttgagcccg gaaaatacaa
     7861 tttacaggtt caactaaata aacagccatt ggcggaagag tacgatattt actggtatgc
     7921 tggtgaagat gacgcgagca aaagctatgc ttgtctgaca ccggaactgg tagcgcagtt
     7981 tggtttaaaa gaagacgtgg cgaaaaatct gcaatggagc cacgatgcta aatgcctgaa
     8041 atccggtcaa ctggaaggca tggaaattaa ggctgattta agccagtccg cattagtcat
     8101 ttcactgcca caggcttacc tcgaatatac ttatcccgac tgggatccgc cttcacgttg
     8161 ggatgacggc atctccggga tcgtcgcgga ctacagcatc aacgcacaaa cccggcacga
     8221 agaaaatggc ggtgatgata gtaacgagat cagcggcaac gggacggtcg gggttaacct
     8281 ggggccgtgg cgtatgcgtg ctgactggca gactaactat caacatactc gcagtaatga
     8341 tgacgatgaa ttcagcggcg atgaaactca aaaaaaatgg gagtggagtc gctactatgc
     8401 ctggcgggcg ttaccatcat taaaagccaa actggcgctg ggcgaggatt acctcagatc
     8461 cgatattttt gatggtttta actatgttgg tggtagtgtc agtactgacg atcaaatgtt
     8521 gcctcccaat ctgcgcggct acgcgccaga catttccggc gtggcacaca ccacagcaaa
     8581 agtgaccgtc agccagatgg ggcgtgtgat ttacgaaacg caggttccgg ctggaccgtt
     8641 tcgtattcag gatcttggtg attctgtctc cggtacgttg catattcgca ttgaagaaca
     8701 gaacggccag gtgcaggaat atgacatcag caccgcctcg atgccatacc tcactcgtcc
     8761 aggtcagatt cgctataaga tcatgatggg ccgtccgcaa gagtggggac accatgtcga
     8821 gggtgaattt ttttctggtg ctgaagcttc ctgggggatc gctaacggct ggtcgttata
     8881 tggcggcgca ctgggagatg aaaactatca gtctgcggcg cttggcgtcg gtcgcgattt
     8941 gtctacattc ggcgcggtcg cgtttgatgt tactcactcg cacaccaaac tggataaaga
     9001 caccgcttat ggcaaaggtt cgctggacgg taactccttc cgtgtgagtt attccaaaga
     9061 ctttgaccag ctcaacagcc gcgttacttt tgctggatat cgcttctcgg aagagaactt
     9121 tatgaccatg agcgagtatc tggatgccag tgacagcgga atggtacgca cgggcaacga
     9181 caaagagatg tacaccgcca cttataacca gaacttccgc gatgcgggtg tttcggttta
     9241 tctcaactat acccgccata cctactggga tcgcgaggag cagataaact acaacatcat
     9301 gctctcgcac tatttcaata tgggcagtat tcgtaatgtc agcatctcga tgactggcta
     9361 ccgttacgag tatgacaacc aggccgacaa aggcatgtac atttcgctca gtatgccgtg
     9421 gggcgacaac agtaccgtta gctataacgg taactatggc agtgggacgg acagcagtca
     9481 ggtcggttat ttcagccgtg tcgatgacgc gactcactat cagttgaacg tcggcaccag
     9541 tgacaaacac accagcgttg acggctatta cagccatgat ggttcgctgg cgcaggttga
     9601 cctcagtgcg aactaccatg aagggcaata cacctctgcg ggcttgtcgt tacagggcgg
     9661 cgcaacgctt actgcccacg gtggcgcact tcaccgtacc cagaatatgg gcgggacacg
     9721 cttgttgatt gatgccgatg gcgttgccga tgttccggtg gaaggtaacg gggctgctgt
     9781 ttataccaat atgtttggta aagccgtcgt ttctgacgtc aataactatt accgcaatca
     9841 ggcgtatatc gacctcaaca gattgcctga aaacgctgaa gcaacccagt cggtggtgca
     9901 agccacgcta actgaaggtg ccattggcta ccgtaaattt gctgtcatta gcggacaaaa
     9961 agcgatggcg gtgctgcgcc tgagcgacgg cagccatcct ccgtttggcg cagaagtaaa
    10021 aaatgataac gagcagacag tgggccttgt cgatgatgac ggcaatgttt atctggctgg
    10081 ggtgaaacct ggcgaacaca tgagtgtgtt ctggagtggt gttgcgcatt gcgatatcaa
    10141 cctgccggac ccgctgcctg ccgatctgtt taacggcttg ttactgccat gccagcataa
    10201 aggcaatgta gcacctatca cttcgccggc ggtcaaaccg gcgattcagg aacagacaca
    10261 gcgggtgacg ccaacggaac ctccgacttc aatttcagta aaccagtaac gtgattaagg
    10321 aatgatccat gtttaatctg actaacactg caaaaatcgt tgtcccggca ctggcgctgc
    10381 tggcgacagc ggtcagtttc tccagccacg cttcggtgac gcccgacagg acacggctgg
    10441 tgtttaacga aagcgacaag tccatcagcg tcacgctgcg caacaacgat cccacactgc
    10501 cttatctggc gcagagctgg atagaggatg caaagggcaa caaaatcagc tctccgcttg
    10561 cggtgcttcc gccagtgcag cgcatcgatg cgatgatgaa cggccaggtg aaggtgcagg
    10621 ggctgccgga tatcaacaaa ctgccggccg accgcgagag cgtgttctat ttcaacgtgc
    10681 gcgaaattcc gccgaaatca aataaaccga acacactgca gattgcgctg cagacgcgta
    10741 tcaaactgtt ctggcgaccg aaggcgctgg agaacaccag catgaaaagt ccgtggcagc
    10801 ataaggtgac gctcatccgt aacggccagg acttcaccgt caataacccg acgccgtact
    10861 acgtgatcat cagcaacgcc agcgctaaaa aaggcggcaa cccgccggcg ggattcacac
    10921 cgctgatgat ggagccgaaa acctccgcac cgctgaatgc gaagatggac agcgtgccgg
    10981 tgctgactta catcaacgac tacggcgcgc ggatgccgct gtacttcaaa tgcaccggca
    11041 acagctgcca ggtggatgaa gagcagagcc gcaaaggctg atgccgggag ggacttatga
    11101 gtcacaagtt tgtagcgggc gcagggttgc tggtgctggc cggatggggc atcagcggtt
    11161 tggctgttgc tggcagtgat atcaaacagg tggatctgac gctgcgaatc ctagtcgatg
    11221 gaccgccgcc ttgctccgtg aagggaagcg ctgtgaaatt tggcaatgtg ataatcaaca
    11281 ggatcgatgg taccaattac caacaggatg cgaaatacac cctcaactgc ggtaataaag
    11341 tcagcgatga cttacggatg cagctcaaag gcaataccgc cgtgatcaac ggagaaacgg
    11401 ttctcagcac ggatatcgcc ggcctggggg tgcggataga aaacgccgcc gacaactccc
    11461 tgtttgcagt tggtgaaaat aactggacgc cttttaatgt caacactcag cctcagctga
    11521 aagcggtgcc ggtaaaacag agcggggtgc aactgactcc gtcggagttt aacgccacga
    11581 tgaccatggt ggtggattac caatgaaaaa attcctgctc cttttactgt gcgctggttg
    11641 cggggctgtg ctggcggcgg aagatgacat cacattccac ggtacgctga tgtcgccgcc
    11701 cgcctgtagc atcagcggcg ggcaaaccat cgaagtgcaa tttcgcgatc tcattatcga
    11761 cagcattgat ggcaattatg gtcgtcagaa agtggactac gaactgagct gcgactcgga
    11821 tgtccgcgat cccgagtgga atatgacact ctcctggacg gggactgcca cctcctacaa
    11881 cgactctgct attgagacgg atgtaccggg cttcggaatt gagcttcagc atgacggtca
    11941 gcgttttaag ctcaacgagc cgctttccat taacgccacc gattttagcc agaagccaaa
    12001 gctggaggcg gtaccggtca aagcggctga tgcggtgctg acggacacga ccttttctgc
    12061 ttacgcaacc ttacgggtgg attaccaatg aaaacagcac acatttattc acccggttat
    12121 gcgctgttag caggcctgct gctgaccgcc agcacaggag cacaggcagt cgacaacaac
    12181 ctgcattttt acggcaacct gctgagtaaa tcctgcacgc tggtggtgga tggtgccaac
    12241 ctggcagaaa tccattttcc caccgtcagc aaccgggatt tgatggtatc tgggcagtcc
    12301 gcgcatctgc cggttgtgtt taagctgaaa gactgcaaag ggccggcggg atacaacgtc
    12361 aaagtcacgc tgagcggcac cgaagacagc ggtcagccgg gttttctggc agtcgatgac
    12421 acctccaccg cgcagggcgt ggggataggg atggaaacgc tcgacggc
//
LOCUS       DAJTYD010000112        12381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTYD010000112 DAJTYD010000000
VERSION     DAJTYD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.112"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            51..995
                     /gene="wecH"
                     /locus_tag="OO585_002789"
     CDS             51..995
                     /gene="wecH"
                     /locus_tag="OO585_002789"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6630319.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1037..1474)
                     /locus_tag="OO585_002790"
     CDS             complement(1037..1474)
                     /locus_tag="OO585_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6630320.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1520..1861)
                     /locus_tag="OO585_002791"
     CDS             complement(1520..1861)
                     /locus_tag="OO585_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630321.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2026..2217)
                     /locus_tag="OO585_002792"
     CDS             complement(2026..2217)
                     /locus_tag="OO585_002792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630322.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2150..3478)
                     /gene="xylB"
                     /locus_tag="OO585_002793"
     CDS             complement(2150..3478)
                     /gene="xylB"
                     /locus_tag="OO585_002793"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6630323.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3595..4872)
                     /gene="xylA"
                     /locus_tag="OO585_002794"
     CDS             complement(3595..4872)
                     /gene="xylA"
                     /locus_tag="OO585_002794"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6630324.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5238..6209
                     /gene="xylF"
                     /locus_tag="OO585_002795"
     CDS             5238..6209
                     /gene="xylF"
                     /locus_tag="OO585_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6630325.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6287..7828
                     /gene="xylG"
                     /locus_tag="OO585_002796"
     CDS             6287..7828
                     /gene="xylG"
                     /locus_tag="OO585_002796"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6630326.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7806..8986
                     /gene="xylH"
                     /locus_tag="OO585_002797"
                     /pseudo
     CDS             7806..8986
                     /gene="xylH"
                     /locus_tag="OO585_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
     gene            9064..10242
                     /gene="xylR"
                     /locus_tag="OO585_002798"
     CDS             9064..10242
                     /gene="xylR"
                     /locus_tag="OO585_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR6630327.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10350..11174)
                     /locus_tag="OO585_002799"
     CDS             complement(10350..11174)
                     /locus_tag="OO585_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6630328.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11494..>12381
                     /locus_tag="OO585_002800"
     CDS             11494..>12381
                     /locus_tag="OO585_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6630329.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTL"
BASE COUNT         3216 a         3064 c         2931 g         3170 t
ORIGIN      
        1 tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga
       61 tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg
      121 atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt
      181 ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct
      241 tatgtctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca
      301 atatggagtt agcgctgaaa aacctgctgc aaaagccagt gttttaccac ttgtggtttt
      361 tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg
      421 gaaaaatgtt gctggtgcta atggcggtga ttggcattat cgctaaccca aacacagtgc
      481 cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt
      541 tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata
      601 aagcactgtc gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt atctctcgcg
      661 ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg
      721 ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata
      781 cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc
      841 acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac
      901 tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg
      961 tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg
     1021 accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg
     1081 ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga
     1141 agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta
     1201 ccatcagtaa tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag
     1261 tggtcggtat gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca
     1321 gtcctaacac cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc
     1381 atagccctaa cagataggta acgataccac caacgagagc tatccatgac acaatactaa
     1441 aggccggtga ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg
     1501 tgtaagagct ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga
     1561 caaaaaaggc gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacac
     1621 cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat
     1681 tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca
     1741 ttaacacgcc aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa
     1801 ccagataaac cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca
     1861 taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa
     1921 aaatgccatt aacaaatgat tttcagaata aattcatact aaatattaat tagtgagata
     1981 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     2041 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     2101 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     2161 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     2221 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     2281 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     2341 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     2401 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     2461 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     2521 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcgaccc
     2581 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     2641 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     2701 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     2761 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     2821 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     2881 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     2941 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     3001 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     3061 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     3121 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     3181 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     3241 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     3301 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     3361 agagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     3421 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     3481 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     3541 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctagcg
     3601 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     3661 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     3721 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     3781 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     3841 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     3901 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     3961 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     4021 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     4081 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     4141 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctggaa
     4201 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     4261 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     4321 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     4381 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     4441 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     4501 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     4561 atccacatcg tggaagcaat aaaatgacac atgtaacttg tggaaaaact caaatgcgac
     4621 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     4681 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt aacaggcggc
     4741 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     4801 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     4861 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     4921 atattgggca ctccctttca gttgctcaat tatgttattt cacactgcta ttgagataat
     4981 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     5041 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     5101 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     5161 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     5221 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     5281 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     5341 tacgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggct gaatctctcg
     5401 gtgcgaaagt atttgtacag tctgcaaatg gcaatggaga aacacaaatg tcgcagattg
     5461 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccatataac ggccaggtat
     5521 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     5581 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     5641 tgcaggcaaa agccctggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     5701 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     5761 acgttgattc cggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     5821 aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca
     5881 ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaattt
     5941 ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga
     6001 cggtgtataa acctattaca ttgctggcaa atactgccgc agaaattgcc gttgaattgg
     6061 gcaatggtca ggagccaaaa gcagatacct cactgaataa tggcctgaaa gatgtcccct
     6121 cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagatacg gtaattaaag
     6181 acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg
     6241 atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact
     6301 tgaaatgaag aacattacca aaaccttcgg cagtgtgaag gcgattgata acgtcagctt
     6361 gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg gtaaatcaac
     6421 gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt
     6481 tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat
     6541 cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg
     6601 taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac gctgtcagaa
     6661 gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct
     6721 tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat
     6781 tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat
     6841 tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt
     6901 caaagcgatt tccgatacga tttgcgttat tcgcgacggt cagcacattg gtacgcgtga
     6961 tgctgccgga atgagtgaag acgatattat caccatgatg gtcgggcgag agttaaccgc
     7021 gctttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg aacatctgac
     7081 ggcatggcat ccggttaatc gtcatattaa acgagttaat gatgtctcgt tttccctgaa
     7141 acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg agaccattca
     7201 gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg atggcaaaca
     7261 ggtagatatt cgtaactgtc agcaagccat cgcccagggg attgcgatgg tacccgaaga
     7321 cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta ccctcgccgc
     7381 actcaataaa tttaccggtg gcattagcca gcttgatgac gcggcagagc aaaaatgtat
     7441 tctggaatca atccagcaac tcaaagttaa aacgtcgtcc cccgatcttg ctattggacg
     7501 tttgagcggc ggcaatcagc aaaaagcgat cctcgcccgc tgtctgttac tcaacccgcg
     7561 cattctcatt cttgatgaac ctaccagggg tattgatatt ggcgcgaaat atgagatcta
     7621 caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct cttccgaatt
     7681 acctgaagtg ctcggcctta gcgatcgtgt actggtgatg catgaaggga aactaaaagc
     7741 caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat tgaggagcga
     7801 acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca tccggtggct
     7861 tttccgggct gaaatcactg aatttgcagg tcttcgtgat gattgcagct atcatcgcaa
     7921 tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt aacgtctcca
     7981 acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg ttcgtcataa
     8041 tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt ggcgtcgcgg
     8101 cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattgtg acgttggttc
     8161 tgggactgct tctcggtgcg tggaacggat ggtgggtcgc gtaccgtaaa gtcccttcat
     8221 ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc atcaccaacg
     8281 gcacgactgt atcccccacc agcgccgcga tgtcacaaat tgggcaaagc tatctccccg
     8341 ccagtaccgg cttcatcatt ggccgcttgg cttaatggct tttgttggtt ggcaatggcg
     8401 cggaagaatg cgccgtcagg ctttgggttt acagtctccg gcctctaccg cagtagtcgg
     8461 tcgccaggct ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg
     8521 tggcgttccc actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc
     8581 aacgcggacg gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg
     8641 tctctccggg attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat
     8701 ggtagccatc gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg
     8761 aaatatcgcc gaactggacg ctattgcagc atgcgtgatt ggcggcacca gcctggctgg
     8821 cggtgtggga agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa
     8881 cggcatgagt atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct
     8941 gttgctggca gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt
     9001 ttctcaaagc cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga
     9061 accatgttta ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac
     9121 cggcaggtag tagaaggcgt aggggaatat ttacaggcgt cacaatcaga atgggatatt
     9181 ttcattgaag aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc
     9241 gtcattgccg acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc
     9301 attgttgggg ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt
     9361 gccaccgata actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt
     9421 aaccgctttg ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc
     9481 gaatatgcat ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg
     9541 ttagaaaccg cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa
     9601 acgctgccac cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg
     9661 caagtatgtg aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgac
     9721 aacgaagaac tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg
     9781 cggcaaatgg gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg
     9841 ccgctacagc gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat
     9901 cgctcgctga ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt
     9961 aaagggatta aagtggatca ggtactggat gcggtcggga tctcgcgctc caatcttgag
    10021 aagcgtttta aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg
    10081 gagaaagcgc gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg
    10141 tgcggttatc catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg
    10201 ccaaaagagt atcgcgatgt aaatcgtgag gtcatgttgt agcgatgatg agaattgtcg
    10261 gcgtcacatc agggaactca gcacagccac ctgatgcgag caaaagcctt atccgaccga
    10321 caaatcagat aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta
    10381 tcctggtaca ttgcgaacag gtagttatta taactcttcc ctttggtcga gtagcccttc
    10441 agcttgtgaa tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc
    10501 gatttacgga acgaagagta agccgggtgc gtattcaggt tagtgacata ggcgctcacc
    10561 gattctttga cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa
    10621 cgtcctttca tgcatttcat gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt
    10681 ccccaaccgc tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg
    10741 cgttcaagca aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag
    10801 cgtttggcga tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt
    10861 tttgaaatca gccagttacg ttctgcagta atggctgcat tttggctggt aatgtaaggc
    10921 attacggtcc ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca
    10981 ggaagtgaac tactctttgc actattgcga gaatactctt gtttactgct tacctgttta
    11041 ttactagctt tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac
    11101 acctcactcg aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg
    11161 ggagtcaaaa tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttc
    11221 acaaatttga gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg
    11281 aatagtccaa atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag
    11341 aaaatgtggg gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct
    11401 tctcatcctg tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact
    11461 gtctgaaatc gcagcaataa ggactcatcc accatgaaac tcgcctcctg ttttctgaca
    11521 ctccttcctg gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc
    11581 gaacagggaa cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc
    11641 acgctaaatt ttgaccaaca gtgctggcag cctgcagatg cgataaaact caatcagatg
    11701 ctttccctgc aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcaaa
    11761 tatacgctgc aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac
    11821 gccgccgaac cggtagcaaa cctggtccgt gaatgcccga aatgggatgg attaccgctc
    11881 acgctggatg tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag
    11941 caaattgcga tagtgaagaa cggtcaaata acgttacaac ccgctgctac cagcaacggt
    12001 ttactcctgc tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc
    12061 acggtttact ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt
    12121 tacggacgtc ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc
    12181 ggcctgatca acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc
    12241 gccccatttg agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat
    12301 tatgcctacc acggctatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac
    12361 gaagccgatc tacggacgct g
//
LOCUS       DAJTYD010000113        12334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTYD010000113 DAJTYD010000000
VERSION     DAJTYD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.113"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..870)
                     /gene="rarD"
                     /locus_tag="OO585_002801"
     CDS             complement(<1..870)
                     /gene="rarD"
                     /locus_tag="OO585_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6630330.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYT"
     gene            complement(922..1395)
                     /locus_tag="OO585_002802"
     CDS             complement(922..1395)
                     /locus_tag="OO585_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6630331.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATATYMVG"
     gene            1560..2429
                     /gene="pldA"
                     /locus_tag="OO585_002803"
     CDS             1560..2429
                     /gene="pldA"
                     /locus_tag="OO585_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6630332.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2562..4391
                     /gene="recQ"
                     /locus_tag="OO585_002804"
     CDS             2562..4391
                     /gene="recQ"
                     /locus_tag="OO585_002804"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6630333.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4455..5075
                     /gene="rhtC"
                     /locus_tag="OO585_002805"
     CDS             4455..5075
                     /gene="rhtC"
                     /locus_tag="OO585_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6630334.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5137..5757)
                     /gene="rhtB"
                     /locus_tag="OO585_002806"
     CDS             complement(5137..5757)
                     /gene="rhtB"
                     /locus_tag="OO585_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6630335.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5868..6890
                     /gene="pldB"
                     /locus_tag="OO585_002807"
     CDS             5868..6890
                     /gene="pldB"
                     /locus_tag="OO585_002807"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6630336.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6898..7698
                     /gene="yigL"
                     /locus_tag="OO585_002808"
     CDS             6898..7698
                     /gene="yigL"
                     /locus_tag="OO585_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6630337.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7774..8673
                     /gene="bioP"
                     /locus_tag="OO585_002809"
     CDS             7774..8673
                     /gene="bioP"
                     /locus_tag="OO585_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6630338.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8561..9514)
                     /gene="metR"
                     /locus_tag="OO585_002810"
     CDS             complement(8561..9514)
                     /gene="metR"
                     /locus_tag="OO585_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6630339.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9750..12011
                     /gene="metE"
                     /locus_tag="OO585_002811"
     CDS             9750..12011
                     /gene="metE"
                     /locus_tag="OO585_002811"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6630340.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
BASE COUNT         2902 a         3136 c         3336 g         2960 t
ORIGIN      
        1 cgtataaatc gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaggtcac
       61 catcttatcg gcacccggtt tttcaccata aaacgtcact gccaacagga acatcagcgt
      121 cgggccaata tactggaaga agcctaacgt ggagagacgc aggcgtgtag cagctgcggt
      181 aaaacacaac agcggtacgg tggtgacaat accggcggcg atcagcagca aattcagcaa
      241 catcgggttt tgccccatat ggctggttga gctgtcggca atagcaaaca gataaatcgc
      301 cgccacgggc agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat
      361 tttcttgcgt accagtccgt agaaggcaaa actaaacgcc agccccagcg cgatgattgg
      421 cagcgaacca aaagtccaca actgaaccaa cacgccacat atcgccagaa tcaccgccag
      481 ccattgcatc cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag
      541 cgggttaata aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat
      601 aaacagtagc cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg
      661 tggcgtctga atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac
      721 cataaagaaa aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta
      781 aatcagcttg aagtatgctg gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa
      841 tacgccctgc cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc
      901 agtttacctg cttttatgtc ttcaacctac catataggtg gcggtggcgg tggcactggc
      961 aatataaagc tgttcttcat tgtgtaattc aacgcgggcg acggcgactt tattgcctgc
     1021 acgcaacagg ctactggtag cagtaaaacg ctcgcccctg cctgggcgca gataatcaac
     1081 gcgaagatca atggtcccca tccgcgatag ccgctggcgt agttcatctt cactgatggt
     1141 ttcgtggcgg gttaaggtac ttcccacgca caccagaccg gcggcgacat ccagcgccga
     1201 cgcaatgact ccgccgtgca aaatgctttg cgcccagtta cccaccatca tcggctgatt
     1261 tttaaaggcc agctgtgcga actctttttc gtaacgctcc agctccatcc ctaacgcgcg
     1321 gttaaatggc atgtgataaa caaacatctc acccactaat ttcagggctt gttcagcggt
     1381 cagtacggca gacatatcat ccttacactt cattggttaa tgaaatgttg attttatgct
     1441 tctttgttgt tggtttctac tttaggaagg gataactaac ggcaatggag ttaagtatgt
     1501 aaaatagccc gcagaaaaac attcacctta tcaataattc gttacggaga acacgaccga
     1561 tgcggactct gcagggctgg ttgttgccgg tgtttatgtt gcctatggca gtatatgcac
     1621 aagaggcaac ggtgaaagag gtgcatgacg cgccagcggt gcgtggcagt attatcgcca
     1681 atatgctgca ggagcatgac aatccgttca cgctctatcc ttatgacacc aactacctca
     1741 tctacaccca aaccagcgat ctgaacaaag aagcgattgc cagttacgac tgggcggaaa
     1801 atgcgcgtaa ggatgaagta aagtttcagt tgagcctggc atttccactg tggcgtggga
     1861 ttttaggccc gaactcggtg ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt
     1921 ccaatagcga agagtcttca ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg
     1981 gttttgccac cgattaccgt tttgcaggtt ggacgctgcg cgatgtggag atggggtata
     2041 accacgactc taacgggcgt tccgacccga cctcccgcag ctggaaccgc ctttatactc
     2101 gcctgatggc agaaaacggt aactggctgg tagaagtgaa gccgtggtat gtggtgggta
     2161 atactgacga taacccggat atcaccaaat atatgggtta ctaccagctt aaaatcggct
     2221 atcacctcgg tgatgcggtg ttgagtgcga aaggacagta caactggaat accggttacg
     2281 gcggcgcgga gttaggctta agttacccga tcaccaaaca tgtgcgcctt tatactcagg
     2341 tttacagcgg ctatggcgaa tcgctcatcg actataactt caaccagacc cgtgtcggtg
     2401 taggagttat gctaaacgac ttgttttgat gaacggttga gtggttggca aatctggaac
     2461 ccagcatcca ggattaccct ctcagagact aaaagcattg cagtttctcg cgcaggcgct
     2521 gaaaatagcg cctgttttta tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt
     2581 tgaatctgga gtccggagct aaacaggttt tacaagaaac ctttggctac caacagtttc
     2641 gccccggcca ggaagaaatt atcgacactg tgctttccgg tcgcgattgc ctggtcgtca
     2701 tgcccaccgg tggcggaaaa tccctttgct atcaaattcc tgccttattg ctaaacggcc
     2761 ttaccgtggt tgtttcaccg ctgatttcgt tgatgaaaga tcaggtggat caactgcaag
     2821 ccaacggcgt ggcggcggcg tgccttaact cgacgcaaac ccgcgagcag caacttgaag
     2881 tgatgacagg ctgccgcacc gggcaaattc gtctgcttta tatcgccccg gaacgcctga
     2941 tgctggataa ctttcttgag catctggcgc actggaatcc ggtgttatta gccgttgatg
     3001 aagcgcactg tatctcccaa tggggccacg atttccgccc ggaatatgcc gcgcttggtc
     3061 agttacgcca gcgtttcccg accctgccgt ttatggcgct gactgccaca gccgacgaca
     3121 ccacgcgcca ggatatcgtg cgcttgctgg ggctgaacga tccgctgatt caaatcagca
     3181 gttttgaccg tccgaatatt cgctacatgc tgatggagaa gttcaaaccg ctcgatcagt
     3241 tgatgcgcta cgtgcaggaa cagcgcggta agtcaggcat tatctactgc aacagccgcg
     3301 cgaaagtaga agacaccgct gcgcgcctgc aaagcaaggg aattagcgcg gcggcctatc
     3361 atgctgggct ggaaaataat gttcgcgccg acgtgcagga aaaatttcag cgcgatgacc
     3421 tgcaaattgt ggtggcgacg gtggcgttcg gcatgggcat caataaacca aacgttcgct
     3481 tcgtggtcca ctttgatatt ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg
     3541 ccgggcgtga tggcctgccc gcggaagcga tgctgtttta cgatccggcc gatatggcgt
     3601 ggctgcgccg ttgcctggaa gagaagccgc aggggcagtt acaggatatc gaacgtcata
     3661 aactcaatgc gatgggcgct tttgccgaag cgcaaacttg ccgtcgtctg gtattgctga
     3721 actattttgg cgaagggcgg caagagccgt gcgggaactg cgatatctgc ctcgatccgc
     3781 cgaaacagta cgacggttca accgatgctc agattgccct ttccaccatt ggtcgtgtga
     3841 atcagcggtt tgggatgggt tatgtggtgg aagtgattcg tggtgctaat aaccagcgta
     3901 tccgcgacta tggtcatgac aaactgaaag tctatggcat gggccgtgat aaaagccatg
     3961 aacattgggt gagcgtgatc cgccagctga ttcacctcgg cctggtgacg caaaatattg
     4021 cccagcattc tgccctacaa ctgacagagg ccgcgcgccc ggtgctgcgc ggcgaatcct
     4081 ctttgcaact tgccgtgccg cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt
     4141 tcggcggcaa ctatgatcgc aaactgttcg ccaaattacg caaactgcgt aaatcgattg
     4201 ccgatgaaag taatgtcccg ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg
     4261 ctgaacagat gccgatcacc gccagcgaaa tgctcagcgt taacggcgtt gggatgtgca
     4321 agctggaacg ctttggtaaa ccgtttatgg cgctgattcg tgcgcatgtc gatggcgacg
     4381 acgaagagta gtcagcagca taaaaaagtg ccaggatgaa gactccgtaa acgtttcccc
     4441 cgcgagtcaa atgtatgttg atgttatttc tcaccgtcgc catggtgcac attgtggcgc
     4501 ttatgagccc cggtcccgat ttcttttttg tctctcagac cgctgtcagt cgttcccgta
     4561 aagaagcgat gatgggcgtg ctgggtatta cctgcggcgt tatggtttgg gcagggattg
     4621 cgttgcttgg cctgcatttg attatcgaaa aaatggcctg gctgcatacg ctgattatgg
     4681 ttggcggtgg cctgtatctc tgctggatgg gttaccagat gctacgcggt gcactgaaaa
     4741 aagaggctgt atctgcgcct gcaccacaga ttgagctggc gaaaagcggg cgcagtttcc
     4801 tgaaaggttt actgaccaat ctcgctaatc cgaaagcgat tatctacttt ggctcggtgt
     4861 tctcattgtt tgtcggtgat aacgttggca ctaccgcgcg ctggggcatt tttgcgctga
     4921 tcattgtcga aacactggcg tggtttaccg tcgttgccag cctgtttgcc ctgccgcaaa
     4981 tgcgccgtgg ttatcaacgt ctggcgaagt ggattgatgg ttttgccggg gcgttatttg
     5041 ccggatttgg cattcatttg attatttcgc ggtgatgcca gacgcgtctt cagagtaagt
     5101 cggataaggc gtttacgccg catccgacat tatttttcac gcatgcctcg ccgatgctaa
     5161 cagcgctccc accagcataa acaacgagcc gaaaatctta ttcagcgcct tcatctgctt
     5221 tggtccttta atccatagag caatccgttg agcaagggtg gcgtaaccga tcatcacaat
     5281 aatatcgact acaatagtgg tgacgccgag cacgatatac tgcatcagtt gcggctgctg
     5341 tggcatgatg aattgcggaa atagcgccgc cagaaacaca atacttttgg gattggtgag
     5401 attcacaaaa actgcgcgct ggaacaaatg tcgacgcgat tgagtagagg caagcgattt
     5461 aaggtcaatt gcaccagcgg cgcgccactg ctggattccc agccaaatca agtaagccgc
     5521 gcctgcccac ttcaacactt caaacgcaat cactgagcgg gaaaatagcg tccccaaacc
     5581 cacgccaacc agcacaatat gaatcgccag tccggtctga agcccagcaa tagacgccac
     5641 cgcgccgcga taaccgtggt tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg
     5701 cgacaggctt aaaatgatcg atgtcagcag gtaggcaaac caccattcta aggtcatgat
     5761 gaactcccgg tgtgtctatt tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg
     5821 cacgctaaaa aaaacgattt tacgtggttt aagaggcaga ttacccgatg tttcagcagc
     5881 aaaaagactg ggaaacaaga gaaaacgcgt ttgctgcttt taccatggga ccgctgactg
     5941 atttctggcg tcagcgtgat gaagcagagt ttactggtgt ggatgacatt ccggtgcgct
     6001 ttgtccgttt tcgcgcacag caccatgacc gggtggtagt catctgcccg gggcgtattg
     6061 agagctacgt aaaatatgcg gaactggcct atgacctgtt ccatttgggg tttgatgtct
     6121 taatcatcga ccatcgcggg cagggacgtt ccggtcgcct gttagccgat ccgcatctcg
     6181 ggcatgttaa tcgctttaat gattatgttg atgatctggc ggcattctgg cagcaggagg
     6241 tccagcccgg tccgtggcgt aaacgctata tactggcaca ttcgatgggc ggtgcgatct
     6301 ccacattatt tctgcaacgc catccaggtg tatgtgacgc cattgcgcta actgcgccaa
     6361 tgtttgggat cgtgattcgt atgccgtcat ttatggcacg gcagatcctc aactgggccg
     6421 aagcgcatcc acgtttccgt gatggctatg caataggcac cgggcgctgg cgcgcgttgc
     6481 cgtttgctat caacgtactg acccacagca gacagcgata tcgacgtaac ttacgcttct
     6541 atgctgatga cccaacgatt cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta
     6601 ttctggctgg cgaacaggtg ttagccggtg cgggtgatga cgccacgcca acgcttctct
     6661 tgcaggctga agaggaacgc gtggtggata accgcatgca tgaccgtttt tgtgaactcc
     6721 gcaccgccgc gggccatcct gtcgaaggag gacggccgtt ggtaattaaa ggtgcttacc
     6781 atgagatcct ttttgaaaag gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt
     6841 ttttcaacag gcataactca cccagcggaa accgctctac agaggtttaa atttcttatg
     6901 taccaggttg ttgcgtctga tttagatggc acgttacttt ctcccgacca tacgttatcc
     6961 ccttacgcca aagaaactct gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg
     7021 accggtcgtc accacgttga tgtggggcaa attcgcgata atctggagat taagtcttac
     7081 atgattacct ccaatggtgc gcgcgttcac gatctggatg gtaatctgat ttttgctcat
     7141 aacctggatc gcgacattgc cagcgatctg tttggcgtag tcaacgacaa tccggacatc
     7201 attactaacg tttatcgcga cgacgaatgg tttatgaatc gccatcgccc ggaagagatg
     7261 cgctttttta aagaagcggt gttcaaatat gcgctgtatg agcctggatt actggagccg
     7321 gaaggcgtca gcaaagtgtt cttcacctgc gattcccatg aacaactgct gccgctggag
     7381 caggcgatta acgctcgttg gggcgatcgc gtcaacgtca gtttctctac cttaacctgt
     7441 ctggaagtga tggcgggcgg cgtttcaaaa ggccatgcgc tggaagcggt ggcgaagaaa
     7501 ctgggctaca gcctgaagga ttgtattgcg tttggtgacg ggatgaacga cgccgaaatg
     7561 ctgtcgatgg cggggaaagg ctgcattatg ggcagtgcgc accagcgtct gaaagacctt
     7621 catcccgagc tggaagtgat tggtactaat gccgacgacg cggtgccgca ttatctgcgt
     7681 aaactctatt tatcgtaatc gttctttatt tggtcagttg tcaacctgat acttcgctac
     7741 aatggatact cgttaatcaa agagttttcc attgtggcgc tacttatcat caccacgatt
     7801 ctgtgggcct tctcctttag cttttatggc gagtaccttg cggggcacgt cgatagctat
     7861 tttgcggtgc tggtgcgcgt tggcctggcg gcactcgttt ttctgccgtt tctgcgtacc
     7921 cgtggcaata gcctgaaaac agtcggcctg tatatgctgg tgggcgcgat gcagctcggc
     7981 gtgatgtata tgctgagttt ccgcgcttat ctctatctga cggtttccga actgctgctg
     8041 ttcaccgtac tgacgccgct ctacatcacg ctgatttatg acatcatgaa taagcgccgt
     8101 ctgcgctggg gatatgcttt tagtgccttg ttggcggtga ttggtgccgg gattattcgc
     8161 tatgatcagg tcaccgacca tttctggact ggcttgttgc tggtgcaact ctccaatatc
     8221 acttttgcca tcggcatggt gggttacaaa cgcctgatgg aaactcgccc gatgccgcag
     8281 cataacgcct ttgcgtggtt ctatcttggc gcgttcctgg tggcggtaat tgcatggttc
     8341 ttgctgggaa atgcgcagaa aatgccgcaa accacgttgc aatggggcat tctggtgttt
     8401 cttggcgtgg tggcttccgg gattggctac tttatgtgga actacggcgc gacgcaggtg
     8461 gacgccggaa cgctgggaat tatgaataat atgcacgttc cggcagggct gctggtaaac
     8521 ctggctatct ggcaccaaca gccgcactgg ccaacgttga ttacaggcgc gctggtgatc
     8581 ctggcctcac tgtgggtgca tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat
     8641 gatcgcaggc gtgattgcgc gctgagcgaa taaacgcttc cgtaactggc tgacgctgct
     8701 cgccatcgcg cacggcggcg tacagtcggc tccacaagcc ttcgcccagg gttttggtca
     8761 ccaccagacc ctggcgctca aaactctcta ctacccaatg cggtagcgcg gcaataccca
     8821 tccgcgcggc aaccatctga atcaacaata aggtgttatc gacgcttttc agtgacggac
     8881 tgacgcctgc cggctgaaga aaatgccgcc agacatccag tcgactacgc tgcaccggat
     8941 aaattaatag cgtctcgctg gcgagatctt ccggtgtaat acgcgttttt gccgccagtg
     9001 gatggtcagg tgctaacacc agacgcactt catagtcgaa catcggcgaa taatgcagac
     9061 cactgcgcgg cagaatatcg gacgtcatta ccagatccag ctctccctgt tgcaaggcgg
     9121 gctgcgggtc aaatgtcacg cccgatttaa agtccatctc gacctgcggc cagttcttat
     9181 ggaaattttc taacgcgggc gtcagccact gtatacagct atggcactca atggcaatgc
     9241 gcagacgcgt ctgctgcggt tcattgcagg cttgcagtgc ctggctaatt tgtggcagca
     9301 cctggtttgc cagttgcaac aggatttctc cctgcggtgt aaagcgaagc ggctggcttt
     9361 tacgcacaaa tagccggaag ccaaggcgtt gttccagatc gctaaactgg tgagacaggg
     9421 cggattgcgt ttgatgcaac gtcgccgcag cggctgcgag tgagccgcag ttccgcaacg
     9481 cttgtagcgt tttcagatgt tttacttcga tcatgaaagt ccttcacttc ggcatgaata
     9541 atttgcgctt gaggaatata cagtaaccgc caattatgga tgtgtaaaca tctggacggc
     9601 taaaatcctt cgtcttttaa atttatggtg cgttggctgc gtttctccac cccggtcact
     9661 tacttcagta agctcccggg gatgaataaa cttgccgcct tccctaaatt caaaatccat
     9721 aggatttaca tataattaga ggaagaaaaa tgacaatatt gaatcacacc ctcggtttcc
     9781 ctcgcgttgg cctgcgtcgc gagctgaaaa aagcgcaaga gagttattgg gcagggaact
     9841 ccacgcgtga agaactgctg gcggtagggc gtgaattgcg tgctcgtcac tgggatcaac
     9901 aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt tgcctggtac gatcatgtac
     9961 tgaccaccag tctgctgctg ggtaacgttc cggcgcgtca tcagaacaaa gatggttcgg
    10021 tagatattga caccctgttc cgtattggtc gtggacgtgc gccgactggc gaacctgcgg
    10081 cggcagcgga aatgaccaaa tggtttaaca ccaactatca ctacatggtg ccggaattcg
    10141 ttaaaggcca acagttcaaa ctgacctgga cgcagctgct ggaggaagtg gacgaggcgc
    10201 tggcgctggg ccacaacgtt aagcctgtgc tgctggggcc ggttacctgg ctgtggctgg
    10261 ggaaagtgaa aggtgaacag tttgaccgcc tgagcctgct gaacgacatt ctgccggttt
    10321 atcagcaagt gctggcagaa ctggagaaac gcggcatcga gtgggtacag attgatgaac
    10381 ccgcgctggt actggaacta ccacaggcgt ggctggacgc atacaaaccc gcttacgacg
    10441 cgctccaggg acaggtgaaa ctgctgctga ctacctattt tgaaggcgtt acgccaaatc
    10501 tcgacacgat tactgcgctg cctgttcagg gactgcatgt ggaccttgta catggtaaag
    10561 atggcgttgc tgaactgcac aagcgcctgc cttctgactg gctgctgtct gcgggactga
    10621 tcaatggtcg taacgtctgg cgcgccgatc ttactgagaa atatgcgcaa attaaggaca
    10681 ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc gttgctgcac agccccatcg
    10741 acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag ctggtttgcc ttcgccttac
    10801 aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc
    10861 tggcagagtg gagcgcccca attcaggcgc gtcgtcactc tactcgtgta cataatccgg
    10921 cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg
    10981 aagtgcgtgc tgaagcccag cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga
    11041 ttggttcctt cccgcaaacc acggaaattc gtaccctgcg tctggatttc aaaaagggca
    11101 atctcgacgc caacaactac cgcacgggca ttgcggaaca tatcaagcag gccattgttg
    11161 agcaggaacg tttgggactg gatgtgctgg tacatggcga ggccgagcgt aatgacatgg
    11221 tggaatactt tggcgagcac ctcgacggat ttgtctttac gcaaaacggt tgggtacaga
    11281 gctacggttc ccgctgcgtg aagccaccga ttgttattgg tgacgttagt cgcccggcac
    11341 cgattaccgt ggagtgggcg aagtatgcgc aatctctgac tgataaaccg gtgaaaggga
    11401 tgctgactgg cccggtgacc attctctgct ggtcgttccc gcgtgaagat gtcagccgtg
    11461 aaaccatcgc caaacaaatt gcgctggcgc tgcgtgatga agtggcggac ctggaagccg
    11521 caggaattgg catcatccag attgatgaac cggcgctgcg cgaaggttta ccgctgcgtc
    11581 gtagcgactg ggatgcatat ctccagtggg gcgtggaggc cttccgtatc aacgccgccg
    11641 tggcgaaaga tgacacacaa atccacactc acatgtgtta ttgcgagttc aacgacatca
    11701 tggattccat tgcggcactg gatgcagacg tcatcaccat cgaaacctcg cgttccgaca
    11761 tggagttgct ggagtcgttt gaagagtttg attatccgaa tgaaatcggt ccaggcgtct
    11821 atgacattca ctcgccaaac gtaccgagcg tagaatggat tgaagccttg ctgaagaaag
    11881 cggcaaaacg cattccggca gagcgcctgt gggtcaaccc ggactgtggc ctgaaaacgc
    11941 gcggctggcc agaaacccgc gcggcactgg cgaacatggt gcaggcggcg cagaatttgc
    12001 gtcggggatg atgaatatta acgagtcact gtaaattgca gtacgatgaa agaggcaaaa
    12061 ctgcctgata ccaggcctac ataatttatg caatttattg aatttttgag ttttgttggc
    12121 cggacaaggc gttcacgccg catccggcat gaacaacgcg cactttgcca ataatctgat
    12181 ggcttccatc accggaagcc attttgctat ctgcgcccgt tcagatacag aaataaccga
    12241 atcattttta cgcaaaatat aatcctgagc gatttgttca gtaatcaacg ctacgttatt
    12301 tattctcact tttcacgaaa ttgtatccct tttc
//
LOCUS       DAJTYD010000114        12323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTYD010000114 DAJTYD010000000
VERSION     DAJTYD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.114"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..765
                     /gene="cysB"
                     /locus_tag="OO585_002812"
     CDS             <1..765
                     /gene="cysB"
                     /locus_tag="OO585_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6630341.1"
                     /translation="REVLSKVDAIKSVAGEHTWPDKGSLYIATTHTQTRYALPNVIKG
                     FIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVMLPCYHWNRAIVV
                     TPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTPRIVFTATDADVI
                     KTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRRSTFLRSYMYDFI
                     QRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1060..1224
                     /locus_tag="OO585_002813"
     CDS             1060..1224
                     /locus_tag="OO585_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6630342.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1227..1394
                     /locus_tag="OO585_002814"
     CDS             1227..1394
                     /locus_tag="OO585_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630343.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1508..1603
                     /locus_tag="OO585_002815"
     CDS             1508..1603
                     /locus_tag="OO585_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630344.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            1767..4442
                     /gene="acnA"
                     /locus_tag="OO585_002816"
                     /pseudo
     CDS             1767..4442
                     /gene="acnA"
                     /locus_tag="OO585_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4506..5096)
                     /gene="ribA"
                     /locus_tag="OO585_002817"
     CDS             complement(4506..5096)
                     /gene="ribA"
                     /locus_tag="OO585_002817"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6630345.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5266..6030
                     /gene="pgpB"
                     /locus_tag="OO585_002818"
     CDS             5266..6030
                     /gene="pgpB"
                     /locus_tag="OO585_002818"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6630346.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6179..6487
                     /gene="lapA"
                     /locus_tag="OO585_002819"
     CDS             6179..6487
                     /gene="lapA"
                     /locus_tag="OO585_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6630347.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6494..7663
                     /gene="lapB"
                     /locus_tag="OO585_002820"
     CDS             6494..7663
                     /gene="lapB"
                     /locus_tag="OO585_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6630348.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            7856..8593
                     /gene="pyrF"
                     /locus_tag="OO585_002821"
     CDS             7856..8593
                     /gene="pyrF"
                     /locus_tag="OO585_002821"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6630349.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8593..8919
                     /gene="yciH"
                     /locus_tag="OO585_002822"
     CDS             8593..8919
                     /gene="yciH"
                     /locus_tag="OO585_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6630350.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9045..9263)
                     /gene="osmB"
                     /locus_tag="OO585_002823"
     CDS             complement(9045..9263)
                     /gene="osmB"
                     /locus_tag="OO585_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6630351.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9532..10281)
                     /locus_tag="OO585_002824"
     CDS             complement(9532..10281)
                     /locus_tag="OO585_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6630352.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10371..10544)
                     /locus_tag="OO585_002825"
     CDS             complement(10371..10544)
                     /locus_tag="OO585_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630353.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(10691..>12323)
                     /gene="pdeR"
                     /locus_tag="OO585_002826"
     CDS             complement(10691..>12323)
                     /gene="pdeR"
                     /locus_tag="OO585_002826"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6630354.1"
                     /translation="EAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLFMS
                     RREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLICSG
                     TDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDNFK
                     KVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMASR
                     ILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQFCV
                     FTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPERGL
                     IPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQTIF
                     TALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGYSS
                     LSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKEDA
                     FLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3114 a         2907 c         3120 g         3182 t
ORIGIN      
        1 cgcgaagtcc tgtcgaaagt cgatgccata aaatcggttg ccggagagca cacctggccg
       61 gataaaggct cactgtatat cgccaccacg catacccaga cacgctacgc attaccaaac
      121 gtcatcaaag gctttattga gcgttatcct cgcgtttctt tgcatatgca ccagggctcg
      181 ccgacacaaa ttgctgatgc cgtctctaaa ggcaatgccg atttcgctat cgccacagaa
      241 gcgctgcatc tgtatgaaga tttagtgatg ttaccgtgct accactggaa tcgggctatt
      301 gtagtcactc cggatcaccc gctggcaggc aaaaaagcca ttaccattga agaactggcg
      361 caatatccgt tggtgacata taccttcggc tttaccggac gttcagaact ggatactgcc
      421 tttaaccgcg cagggttaac gccgcgtatc gttttcacgg caacggatgc tgacgtcatt
      481 aaaacttacg tccggttagg gctgggggta ggggtcattg ccagcatggc ggtggatccg
      541 gtcgccgatc cggaccttgt gcgcgttgat gctcacgata tcttcagcca cagtacaacc
      601 aaaattggtt ttcgccgtag tacttttttg cgcagttata tgtatgattt cattcagcgt
      661 tttgcaccgc atttaacgcg tgatgtcgtt gatgcggctg tcgcattgcg ctctaatgaa
      721 gaaattgagg tcatgtttaa agatataaaa ctgccggaaa aataatttct tgcgttattt
      781 tcggcacctt ttatgtagcg aaggtgccgg aatatattct cttttgttac ttatttttat
      841 accgtgcaca atatttttat ccctcatatt tatagggtaa attacctgta aagtagtgtc
      901 taattacccg cctcgcaaat tccagttttc aaatatctat ttcaggtcaa aagattgaat
      961 tattcactgg agacgattcg taaattcgct ggatttttga ctaaagtttc tttaggattt
     1021 gtctcatcgt atgattaatt acaccaatcg ttcaacgtct tgctgataag tgatggcgat
     1081 tatatgaggt tagcaatgcc ttctggaaat caggaacccc gccgcgatcc tgaattaaaa
     1141 cgtaaagcct ggctggcggt ttttcttggt tctgcacttt tctgggtggt tgtcgcactg
     1201 ctgatttgga aagtgtgggg ataactatgg tcggtcagga gcaactggag tcgtcaccat
     1261 tatgccagca tagtgacaat gaaacagaaa cgaaacggga atgttccgtc gttattccag
     1321 acgactggca actaacatcg cagcagcaag cctttataga actgtttgct gaagatgatc
     1381 agccgaaaca ataattatca tcattcttat tacccatttt taatgaatta aagggctttt
     1441 aatacaccgc agcaataaca gcttgagtta tctcaacaca aaataataac cgttaagggt
     1501 gtagcctatg atcaacacaa atatgaaata ttggtcctgg atgggcgcgt tttctctgtc
     1561 gatgctcttc tgggccgaac tcctctggat cattactcac tgatccttga ccccgctgcg
     1621 gcggggttgt catttgcttt gccacaaggt ttctcctctt ttatcaattt gggttgttat
     1681 caaatcgtta cgcgatgttt gtgttatctt taatattcac cctgaagaga atcagggctt
     1741 cgcaaccctg tcattaagga ggagctatgt cgtcaaccct acgagaagcc agtaaggaca
     1801 cgttgcaggc caaagataaa acttaccact actacagcct gccgcttgct gctaaatcac
     1861 tgggcgatat cacccgtcta cccaagtcac tcaaagtttt gctcgaaaac ctgctgcgct
     1921 ggcaggatgg taactcggtt accgaagagg atatccacgc gctggcagga tggctgaaaa
     1981 atgcccatgc tgaccgtgaa attgcctacc gcccggcaag ggtgctgatg taggacttta
     2041 ccggcgtacc tgccgttgtt gatctggcgg caatgcgcga agcggttaaa cgcctcggcg
     2101 gcgatactgc aaaggttaac ccgctctcac cggtcgacct ggtcattgac cactcggtga
     2161 ccgtcgatcg ttttggtgat gatgaggcat ttgaagaaaa cgtacgcctg gaaatggagc
     2221 gcaaccacga acgttatgtg ttcctgaaat ggggaaagca agcgttcagt cggtttagcg
     2281 tcgtgccgcc aggcacaggc atttgccatc aggttaacct cgaatatctc ggcaaagcag
     2341 tgtggagtga attgcaggac ggtgaatgga ttgcttatcc ggatacactc gttggtactg
     2401 actcgcacac cactatgatc aacggccttg gcgtgctggg gtggggcgtg ggtgggatcg
     2461 aagcagaagc cgcaatgtta ggccagccgg tttccatgct tatcccggac gtagtgggct
     2521 ttaaacttac cggtaaatta cgtgaaggta ttaccgccac agacctggtt ctcactgtta
     2581 cccaaatgct gcgcaaacat ggcgtggtgg ggaaattcgt cgaattttat ggtgatggtc
     2641 tggattcact accgctggcg gatcgcgcca ccattgccaa tatgtcgcca gaatatggtg
     2701 ccacctgtgg cttcttccca atcgatgctg taaccctcga ttacatgcgt ttaagcgggc
     2761 gcagcgaaga tcaggttgag ttggtcgaaa aatatgccaa agcgcagggc atgtggcgta
     2821 acccgggcga tgaaccgatt tttaccagta cgttagaact ggatatgaat gacgttgaag
     2881 cgagcctggc agggccaaaa cgtccacagg atcgcgttgc actgcccgat gtaccaaaag
     2941 catttgccgc cagtaacgaa ctggaagtga atgccacgca taaagatcgc cagccggtcg
     3001 attatgttat gaacggacat cagtatcagt tacctgatgg cgctgtggtc attgctgcga
     3061 taacctcgtg caccaacacc tctaacccaa gtgtgctgat ggccgcaggc ttgctggcga
     3121 aaaaagccgt aactctgggc ctcaagcggc aaccgtgggt caaagcgtcg ctggcaccgg
     3181 gttcgaaagt cgtttctgat tatctggcaa aagcgaaact gacaccgtat ctcgacgaac
     3241 tggggtttaa ccttgtggga tacggttgta ccacctgtat tggtaactct gggccgctgc
     3301 ccgatcctat cgaaacggca atcaaaaaag gcgatttaac cgtcggtgcg gtgctgtccg
     3361 gcaaccgtaa ctttgaaggc cgtatccatc cgctggttaa aactaactgg ctggcctcgc
     3421 cgccgctggt ggttgcctat gcgctggcgg gaaatatgaa tatcaacctg gctgctgagc
     3481 ctattggtca tgatcgtaaa ggcgacccgg tttatctgaa agatatctgg ccatcggcac
     3541 aggaaattgc ccgtgcggta gaacaagtct ccacagaaat gttccgtaaa gagtacgcag
     3601 aagtttttga aggcacagca gagtggaagg aaattaacgt cacacgatcc gatacctacg
     3661 gttggcagga ggattcaacc tatattcgct tatcgccttt ctttgatgaa atgcaggcaa
     3721 caccagcacc tgtggaagat attcacggtg cgcggatcct cgcaatgctg ggggattcag
     3781 tcaccactga ccatatctct ccggcgggca gtattaagcc cgacagcccg gcgggtcgat
     3841 atctacaagg tcggggtgtt gagcgaaaag actttaactc ctacggttcg cggcgtggta
     3901 accatgaagt gatgatgcgc ggcaccttcg ccaatattcg catccgtaat gaaatggtgc
     3961 ctggcgttga aggggggatg acgcggcatt tacctgacag cgatgttgtc tctatctatg
     4021 atgccgcgat gcgctataag caggagcaaa cgccgctggc ggtgattgcc gggaaagagt
     4081 atggatcagg ctccagccgt gactggacgg caaaaggtcc gcgtctgctt ggtattcgtg
     4141 tggtgattgc cgaatcgttt gaacgaattc accgttcgaa tttaattggc atgggcatct
     4201 tgccgctgga atttccgcaa ggcgtgacgc gtaaaacgtt agggctaacc ggggaagaga
     4261 agattgatat tggcgacctg caaaatctac aacccggcgc gacggttccg gtgacgctta
     4321 cgcgcgcgga tggtagccag gaagtcgtac cctgccgttg tcgtatcgac accgcgacgg
     4381 agttgaccta ctaccagaac gacggcattt tgcattatgt cattcgtaat atgctgaagt
     4441 aacaagtatt tgcttgccgg ttattttgct tccggcaagc aaattaattc cacaatgcga
     4501 gagggttatt tgttcagcaa atgacccatt ttctcggctt tggtatcgag ataatgttcg
     4561 ttattggggt tacgacctac aatcaatggt acgcgttcaa caatattaat ccctgcttcg
     4621 gtcagaattt cgactttttt cgggttattg gttaacaagc ggacttcatt gacgccaagg
     4681 agtttgaaca tatcagcgca aagagtgaag tcgcgctcat cagcggcgaa gcctaactgg
     4741 tggttagcct ctacggtatc gtaaccttga tcctgcagtg cgtaagcgcg gattttattc
     4801 agcagaccaa tgttacgacc ttcctgacgg tgatacagca aaataccacg gccttcctcg
     4861 gcaatttgcg tcaatgccgc ttcgagctgg aagccacaat cgcagcgcaa gctgaacagg
     4921 gcgtcaccgg tcagacattc ggaatggacg cgcgcaagta ccggggtatg cccggaaata
     4981 tcgccataga ctagcgcgac atgatcgtgt ccggttgcca gttcttcaaa tcccaccatc
     5041 aggaaatcgc cccatggggt tggcagtttg gcttctgcca cacgtttaag ctgcatgaaa
     5101 ttctccagat aatgctggtt ctgtattggc ttattttgcc ataacgagaa ggggttcacc
     5161 taatcacgac gcgtcgatcg ttcacggaat ggcacaaatc tgtcaatttt tcctggaact
     5221 ggcgttttca gttatgattg tgggacttat caaaaaggag aggccatgcg ttcgattgcc
     5281 agacgtaccg cagtgggagc tgcactattg cttgtcatgc cagtagccgt atggatttct
     5341 ggctggcgtt ggcaacctgg agaacaaagt tggctactaa aagcggcttt ttgggttact
     5401 gaaactgtca cccagccctg gggcgtcatt acacatttga ttttattcgg ctggtttctc
     5461 tggtgtctgc gttttcgcat taaggctgcc tttgtattat ttgccattct ggcggccgca
     5521 atccttgtgg gacaaggcgt taaatcctgg atcaaagaca aagtccagga accacgacct
     5581 tttgttatct ggctggaaaa aacacatcat attccggttg atgagttcta cactttaaag
     5641 cgagcagaac gcggaaatct agtgaaagaa cagttggctg aagagaaaaa tatcccacaa
     5701 tatttgcgtt cacactggca gaaagagacg gggtttgcct ttccttccgg tcacacgatg
     5761 tttgctgcca gttgggcact gctggccgtt ggtttgctgt ggccgcgtcg gcgaacgtta
     5821 accattgcta tcttgctgct ctgggcaacg ggagtcatgg gaagccgcct gctgctcggg
     5881 atgcattggc cacgcgatct ggtagtagct acattgattt cgtgggcgct ggtggcggtg
     5941 gcaacgtggc ttgcgcaacg aatttgtggg ccattaacac cacctgcgga agaaaatcgc
     6001 gaaatagcgc aacgagaaca agaaagttaa cgctggttga ttttccgaat ttagccctta
     6061 aatcatcaac aatgcgcgtg gatgccattt cgcagacggc gcgaaaatgg tactttaaag
     6121 ggctattgcg gtaagttgac cataatttat tcgctctaac cacataacgg gaagtaatgt
     6181 gaaatattta ctcattttct tactggtgtt agcgatcttc gtgatttcgg tcacgttggg
     6241 tgcgcagaac gatcaacagg tgacgtttaa ttatctgtta gcgcaagggg agtaccgtat
     6301 ttccacattg ctggcggtat tgtttgctgc ggggtttgct atcggttggt tgatttgtgg
     6361 cctgttctgg ctgcgagttc gtgtttccct ggcgcgcgct gaacgtaaaa taaagcgact
     6421 ggaaaaccag ctttcacccg cgactgacgt ggctgtagtg ccgcactcgt cagcggcgaa
     6481 ggaataactt tctatgctgg agttgttgtt tctgcttttg cctgtagccg ctgcctatgg
     6541 ctggtatatg ggccgcagaa gtgcgcaaca aaacaagcaa gatgaagcca accgcttgtc
     6601 gcgtgattac gtagcggggg ttaacttcct gcttagtaat caacaggata aagcggtaga
     6661 cctgtttctc gatatgctta aagaggatac aggcaccgtt gaagcccacc ttacgctcgg
     6721 aaacctgttc cgttcgcgtg gcgaagttga tcgcgctatt cgcatccatc agaccctaat
     6781 ggaaagcgcc tcgctgacct atgaacagcg tctgttggcg attcaacaac tagggcgtga
     6841 ttacatggcc gccgggttat acgaccgcgc ggaagacatg ttcaatcagc tgaccgatga
     6901 aactgacttc cgcattggcg ctctgcaaca gttgctacaa atctaccagg ctaccagtga
     6961 gtggcagaaa gcaattgatg ttgccgaacg cctggtgaag ctgggtaaag ataaacagcg
     7021 cgtcgaaatt gcccatttct actgtgagtt agccctgcag catatggcca gcgacgatct
     7081 cgatcgtgcc atgaccttgc taaaaaaagg ggcggcggca gataaaaaca gagcccgcgt
     7141 atccatcatg atgggacgcg tgtttatggc gaaaggagaa tacgccaaag ccgtcgaaag
     7201 tttgcaacgc gtgatatccc aggacagaga actggtcagc gaaacgctgg aaatgttgca
     7261 aacctgctat cagcagttgg gtaaaactgc cgaatgggca gaattcctgc aacgcgcggt
     7321 ggaagagaac accggtgccg atgctgaatt gatgcttgct gatatcatcg aagcgcgcga
     7381 cggtagtgag gccgcacagg tctatattac gcgccagctt cagcgtcatc cgaccatgcg
     7441 tgtgttccat aagttaatgg attaccactt aaatgaagcg gaagaagggc gtgccaaaga
     7501 gagcctgatg gtgctgcgtg acatggttgg cgaaaaggta cgtagtaagc ctcgttatcg
     7561 ctgccagaaa tgtggtttta ccgcatacac cctctactgg cattgtccgt cttgtcgggc
     7621 ctggtcaacc attaaaccga ttcgcggtct tgatggcctg taatttttaa aaaaatccga
     7681 ctttagttac aacatgctag ttattgatgt tccattgtgc tccggcaacg acggagcaga
     7741 aaaagcctgc caggggaaaa atcgcaactg ttaatttttt atttccacgg gtagaatgct
     7801 cgccgtttac ctgtttcgcg ccacttccgg tgcccatcat caagaaggtc tggtcatgac
     7861 gttaactgct tcatcttctt cccgcgctgt tacgaattct cctgtggttg ttgcccttga
     7921 ttatcataat cgtgatgacg cgctgtcctt tgtcgacaag atcgacccac gcgattgtcg
     7981 tctgaaggtc ggcaaagaga tgtttacatt gtttgggcca cagtttgtgc gcgaacttca
     8041 acagcgtggt tttgatatct ttcttgacct gaaattccac gatattccta acactgcagc
     8101 gcacgctgtc gctgctgcag ctgacttagg cgtgtggatg gtgaatgttc atgcctctgg
     8161 tggggcgcgt atgatgaccg cagcgcgtga ggcactggtt ccgtttggca aagatgcacc
     8221 gcttttgatt gctgtaacag tgttgaccag catggaagct agcgacctgg ccgatcttgg
     8281 cgtgacactg tcacctgcag attatgcaga acgtctggcg gcactgacgc aaaaatgtgg
     8341 ccttgatggt gtggtgtgtt ctgctcagga agctgtgcgc tttaaacagg tattcggtca
     8401 ggagttcaaa ctggttacac cgggcattcg tccgcagggg agtgatgctg gtgaccagcg
     8461 ccgcattatg acgccagaac aggcgttggc ggctggtgtt gattatatgg tgattggtcg
     8521 cccggtaacg caatcggtag atccagcgca aacactgaaa gcgatcaacg cctctttaca
     8581 gcggagtgca tgatgagtga ttccaacagc cgtctggtct actcaacgga aaccggacgt
     8641 attgatgagc caaaagcggc cccagtacgc cctaaaggcg atggtgtggt gcgtattcag
     8701 cgtcagacca gtggacgtaa aggtaagggc gtttgcctga ttaccggtat cgatctcgat
     8761 gatgccgaac tgacaaaact tgcagcggaa ctgaagaaaa aatgcggctg cggcggagca
     8821 gttaaagatg gagttattga aatccagggc gataagcgtg atttattaaa atcactactt
     8881 gaagcgaaag ggatgaaggt aaaactcgca ggcggttaac ataaaaagcc acggatatat
     8941 ccgtggcttt cgaatatttt actgtgtgta ttattcagtt atttcgtact gaacaaaatc
     9001 aagcgtatta taattgcgag tcgataatcg acatttattt acgattattt accgacctgg
     9061 tgaccaataa caccaccgac agctgcacca cctaatgtac ccaacgtact gccatcggtc
     9121 agtactgcac cgcctaatgc ccctgcaccc gcgccgattg cggtgttgcg gtcccgttta
     9181 gaccagttag aacaggcact cagagacatt gccaaagtaa ttgccagaac agccgcggtc
     9241 atttttttgc tcgttacaaa cataatactc tctcctgaat ttatgattca cggaagtaag
     9301 ctctctataa ctataatagc taagaataag cctggtgaaa ttattccgtg aaatctgctc
     9361 gcgcgcaggg ttatatcacg caggtgataa tgacttcctg ttatatcgct gataataatt
     9421 ttatatctta agcgtgttaa taacaggtaa atagtcttaa ttatcaacca ggaatcatct
     9481 tagagcggat gatttgccaa actgcaaatc atccgtagag aagggaaatg gttaaatgtc
     9541 aacgacgtga acggtgagtt tactgtgctc aagatgcatc agatcgctgg cgcgtatttt
     9601 cgaatcggta atcacgcgat tgaatcgctc aacgggaccg atggagtatg aatgtacagc
     9661 accaaatttc gagctgtcag tcaggactat cacttcgcac tctttttcca gcacggcatt
     9721 gaccacatcg gtacgcatca tatcgcgacc ggtgaatcca gtttcaggtt gccagccatc
     9781 aataccaata aatgctttgc tgaaatgcac ctgctggatg cactggcgtg tcaacgggcc
     9841 aaccatactt tcgctttttt tctggtacac gccaccgagc aaaatgactt cacaaggcgc
     9901 gtctttcagc aaatgcgcga tgtagctgct gaccgtgatg atagtgacat ttttcttctg
     9961 ctcccccaga gtccgggcga gcagggcatt gctgctgcca ttttcgataa agatggtttc
    10021 gcccggttga accagtgacg cggcaaactc ggcgagttca cgcttcagcg tatagttgct
    10081 catcatacgg gtttcgacgt catcactatc aagcgaaact gcaaagccat gtgcacggcg
    10141 gaggtaactc agtttttcga gggtgttgag atcctggcga atggtaactt cagaaactcc
    10201 agtggctttt gccagatcgg ttacgctaac ctgaccctgg tcaatgacca tctgtagaat
    10261 agtttgttgt cgggaattca tcgctgtaat tcttataacg ttataatact aaataaaaaa
    10321 atactgacgc gccgggcatc ctcaaatagg aaaacctgaa ggcacgattg ttaaatctcc
    10381 cacggtgcct tcgggatgtc tggcgtagat tcagcaacct ggcgcaagag ttctgcttta
    10441 agtatctcga gattatggat cgcagagtgg taatcgcctg ccacaaggat atctaacaca
    10501 atatcaatac gttcggcaac gcgctgggca tcaaattcag acatcaagga catccttata
    10561 gcaaaagtgg aaaatgaata atgcaaaaat tgccgctaaa agagaagtgt ttaacagcaa
    10621 cggctaatta tcatccagga atacgatata taatgacggg atatagcgct aagtatatat
    10681 attcatctac ttatgcgcgc ttcagatagc gtttatacca gcgttcgaag gcgacggcgg
    10741 gcatcggttt ggcaaacaaa aatccttgcc gctcattgat cccgttcttg gttaaaaaag
    10801 catcttcctt tgcactctct acaccttcgg cgatcacctg aagattcaat gcctgggcca
    10861 cagcgacgat cgcccggacc agtgactgcg agacaggttg tttgtgaata tctcgaacaa
    10921 aaacctggtc aagtttgatg gcatcgatcg gaaagcgcgc cagttgcgaa agtgaagagt
    10981 agccggtacc aaaatcatcc agatgcactt gcgcacctag ttggctaaat tgttgaataa
    11041 cagacagtgc cagttcatca ttctcaatca gacaactctc tgtcagttca acatctatag
    11101 ggcagtattc aaaattgagt tcctggagaa cctgtttcag ggcggtgaaa atggtttgat
    11161 cggcgagctg acgtgcagaa atatttaccg ccactcgcag gtttatgcct ttatcccgcc
    11221 actttgccac ctggcgtacg acatcgagaa tcacccaacg gcctaaaggc acaattagcc
    11281 ctgactcttc ggcgtaggaa atgaagtcca acggtggaat caacccacgt tcaggtgact
    11341 gccaacgtac tagtgcttcc agactgcgca cttcgccacg ccaggtgatt ttcggttgat
    11401 agtgaataac caactgatcg ttttccagtg ctttacgcaa gttggtatcc agccagagat
    11461 attcaaatac ccgttgattc atttctgggg taaaaacgca aaattgtcct cgtccgcctt
    11521 ccttcgctgt gtacattgct gtgtcggcgt gacgaataat agccgtgctg tctgaaccat
    11581 gttcgggaga gagtgcaata cctactgaac agctggtata aacttcaatt aaaccaatgc
    11641 gaaaggggag ccgtaagcgg gtcaaaattc gtgatgccat tgcttccagc gcgctttgtg
    11701 aggtgttgga tgccagtacc agaaactcat ccccacctgg acgcgccaac acctggtcat
    11761 gttcgagaca gcttaaaata gccaatgaca cgtcgcgtaa taactggtca ccaaacaaat
    11821 gcccataggc gtcgttgacc tttttgaaat tatccaaatc aagatacaca accccaactt
    11881 tattgttatc tgcatgatta atagcgtgat cgattaaatc ctgcattgcg ttacgattcg
    11941 gcagtccggt gatactgtcg gtatttgcca gaatacgcag tcgctcctga gcgcggcgct
    12001 cttcggtaat gtcggtgccg gaacagatta aaaaaatctc gcttttgcca ctgccgctgt
    12061 ggacaaattt attgcgaaac agaaacagcc gctggccttt acatgttggt atccacagtt
    12121 cgacttcata tgcattgccg cttcgaaaaa atacacggtt attgcgcctg gatgccgcag
    12181 cttcacgacg gctcataaac agtttaaaca cgctttggcc aatgacgtcg tgttctttca
    12241 acccagtgta atcttcacat aaccgattga agcgttggat attcccccgg ctgtcgagaa
    12301 tcactattgc ggaatgtgct tca
//
LOCUS       DAJTYD010000115        12150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTYD010000115 DAJTYD010000000
VERSION     DAJTYD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.115"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(30..1739)
                     /locus_tag="OO585_002827"
     CDS             complement(30..1739)
                     /locus_tag="OO585_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6630355.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(1860..3251)
                     /gene="xanP"
                     /locus_tag="OO585_002828"
     CDS             complement(1860..3251)
                     /gene="xanP"
                     /locus_tag="OO585_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6630356.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            3531..4736
                     /gene="gltS"
                     /locus_tag="OO585_002829"
     CDS             3531..4736
                     /gene="gltS"
                     /locus_tag="OO585_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6630357.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(4770..6851)
                     /gene="recG"
                     /locus_tag="OO585_002830"
     CDS             complement(4770..6851)
                     /gene="recG"
                     /locus_tag="OO585_002830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6630358.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(6857..7546)
                     /gene="trmH"
                     /locus_tag="OO585_002831"
     CDS             complement(6857..7546)
                     /gene="trmH"
                     /locus_tag="OO585_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6630359.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(7553..9661)
                     /gene="spoT"
                     /locus_tag="OO585_002832"
     CDS             complement(7553..9661)
                     /gene="spoT"
                     /locus_tag="OO585_002832"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6630360.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(9680..9955)
                     /gene="rpoZ"
                     /locus_tag="OO585_002833"
     CDS             complement(9680..9955)
                     /gene="rpoZ"
                     /locus_tag="OO585_002833"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6630361.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(10010..10633)
                     /gene="gmk"
                     /locus_tag="OO585_002834"
     CDS             complement(10010..10633)
                     /gene="gmk"
                     /locus_tag="OO585_002834"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6630362.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            10891..>12150
                     /gene="ligB"
                     /locus_tag="OO585_002835"
     CDS             10891..>12150
                     /gene="ligB"
                     /locus_tag="OO585_002835"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6630363.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVSL"
BASE COUNT         2708 a         3188 c         3327 g         2927 t
ORIGIN      
        1 gtctgagtcg tacccggcga gattgatgtt tactgcacca gctctgccga cgtaacttca
       61 ccgctggaaa cgatgccagc attcatggtt tgagtcactt gctgctttgc ggcgttcacg
      121 gcatgcaatt gcccgctaac cgtaggtttc agcggggcat tggcctgaat atctccactg
      181 gcggtaagct gaatattgcc gtcgccagtc aacggtaatt caggccatcc ccattgttgc
      241 aaaatattca ccggcacacc gcgtccattc aggctgatat gggtctgacg ttgtggcgtt
      301 tgtgaaacac tggcagtggc ttccagaatg cctttttcag taaatgcact cagttcgctg
      361 atattcaccg tgctgctgtt ggcggtcagc gccagcgacg ggcgacgaac atcaacacga
      421 ttgaatgtcg cggcggcggc attcagattc gccgagccac tccagacgcc ccatttatga
      481 tcggtaacca gcgtcaggtt ggcaccgtaa ccatcgagcg tggtgagctg ccacgggaag
      541 tcagggtcga tatcaatgat cagattgcgg ctggcgctaa atctcttcag ttgcaggctg
      601 tttaaccaac cgggtgtcgt ttccatccac aactgttgcc agtttttcgg caaggtatat
      661 tccagcccgg caattgccgc atcatcaagg atcaacgttt tcccgtcacg cagccagttc
      721 cctgacgttc tgaccatacc cccttcccag cggctggtga actggcgcag cgctacgccc
      781 tgcggggaaa attccgcgtt tataatcggg tcaaataaat gcagcgaacc ataaatgaac
      841 tcgctagcgt tcatcgacag tttgccttct tgtgtctgcc agtcatcttt actgaaggtc
      901 atgttgcgca agctgagatc gaggtcggtc accgcccagt ccggaccttg caaatgagca
      961 tcgatcactt ccaggcgacc aatctgcaac gacgggacag agcgtaatgg cgcaaagaag
     1021 tcggttagcg atttttcgct ttgtagacgt atatccgcca tgcgcaggtt ttccacttgc
     1081 cagctgccgt cggcgttacg ctgcgcgttt ccggttaatg tcccgcgggc gatgtcggca
     1141 cccaggttag tcagcgtaac gcgatcgtta tcaatactgc cttcaatcag tacattggtg
     1201 gcaggaacat cattgagcga aagcgatccg gcactaaact gaatctgcgc cttcgtaccc
     1261 agcactttac cggcttttgg tgaccacgga actacgccgc catttacccg ctgcgcgctc
     1321 agtttccatt cgctattcgg gctattaaac gccatatcac gcagttgcag acgatcggct
     1381 ttgaacggta gcggcgcggt ctggtcggtg agattcagcg tcccgttttc cagcaggatg
     1441 gtatcgacat ggcgtggttc ggtcagttgc cgactgctta gcgcaatgtc gacacttttg
     1501 gccaccaggg tcgcgggctg accatcacga ccaaacgtga cattctccag cacgatatga
     1561 gatggcgcgg aaaaacggtg atccatcgcc ccgaaggcca gatgatagtc gctattctcg
     1621 gaaacccatg cgctgatatg ttctgctccc cagcgggttt gcagaagaaa atagaggcca
     1681 gcgatcacca ctaacagagc gatgagaatg tagagaagca gcttcccaat aaatttcatg
     1741 gtcttccatt ccgtgaaatg cacataagag agttatgcac gatttacgcg caatcctcaa
     1801 ggcgggaatg gtgaaagagg tgagactgcg gcggtaatta tcttaccgcc gccagtgaat
     1861 tactgttttt ctggtgggaa aatcagattc agcacgatgg cagtaatacc gcccgcggcg
     1921 atcccggagg agagcaggtt tttcagccat tcaggggcaa actgcaaaat cagcggctgc
     1981 tgagacacgc ccagaccaac cgccagcgac agcgcgataa tcagaatcgc ccgacggttc
     2041 agcggctcac gagaaacgat acgcacaccg gaggcggcga tggtgccaaa cattacaagc
     2101 gttgcgccgc ccagaaccgg ttctggaatg tgttgtacaa aaccgctcac tgccgggaac
     2161 agacccagca cgatcaacat cagcgcgacg acaaaaccga catagcggct ggcaacacca
     2221 gtcaactgga tcactccgtt gttttgcccg aagcaggagt tcgggaaggt gttaaacacc
     2281 gccgaaacaa acgagttcag gccgtttgcc agcacgccgc ctttcaggcg tttcatgtac
     2341 agcggaccgg acactggctg ttcggaaacg tcagaggtcg acgtgatatc gccaatggtt
     2401 tccagcgaag tgatcataaa gaccagcatc agcggcagca gcagactcca ttcaatgcca
     2461 agaccgtaat agagcggcgt tggcaccata atcagttctt gcgtcatcgg ttcgttgctt
     2521 tctggcaaca tgcccataaa ccacgccagc gcatatccgg ccgccatcgc aatcaccagt
     2581 gaggccacgc gtaagtaagg gttacgttga cggttaagca ggataattaa ggctaagacc
     2641 acgcctgcca gcagcagatt tttcggtgcg ccgaaggtgt tatcgctcat ggctgcgtaa
     2701 ccgccgccaa tggacgttaa cccaacctga attagcgaca ggccgataat catcaccaca
     2761 acgccagaaa ccagcggcgt aataatgcgg cgcgccagat gcagaacgcg ggagatcacc
     2821 atctcggtgc aacttgccag catcaacgtg ccgaacaaag ccgccatcat ggtaggaaca
     2881 tcagcaccac cggttttcag cgcggtaccg cccataatca gcggggcaac aaagttgaag
     2941 ctggtgccct gaatagacaa cagcccggag ccaaccggac cccaggtctt aatttgaata
     3001 atcgatgcca caccggaggc aaacagcgac atactaataa tgtgttgcgt gtcttgtgcc
     3061 ggtaaaccca gcgcctggca gattaatagc gctggcgtga tcaccgcaac gaacatcgcc
     3121 agcagatgct gacaggcggc aaacagggtt tgaggaagcg gcggacgatc ttcaagacgg
     3181 taaatcagtt cgctgttttg agtctgcgca accggttgcg cattttctga ctcgagggtg
     3241 gaaacagaca tcgaaaacaa tcccgtggta gcaaagcggg cattttagct gaccgaaaga
     3301 gaaaagcaaa cgtttgccac atcctcatca aaatatttaa atgagataaa tgcacttttt
     3361 tatataactt ttggtatttt tctgcttaaa atccatgcca tttgttcatt aacagctgag
     3421 aatttgaccc aggaaagcat aactcgatac ccgtcatagt tcatgaagtt gccactgcgc
     3481 gatgaagtat gacgagtatg aaagagtgat gcggatacaa aggagtaact atgtttcatc
     3541 tcgatacttt agcaacgctt gttgccgcaa cgctgacgtt gctgctcggg cgtaagttgg
     3601 tccattccgt ctcctttttg aagaaataca ccataccgga acctgttgcg ggtggtttgt
     3661 tggtggcgct ggcgctacta gtactgaaaa aaagcatggg ctgggaagtc aactttgata
     3721 tgtccctgcg cgatccgtta atgctggctt tcttcgccac cattggcctg aacgccaaca
     3781 ttgccagttt gcgtgccggt gggcgtgtgg ttggcatctt cttgattgtg gttgttggtc
     3841 tgttggtgat gcaaaatgcc attggcattg gtatggctag cctgttaggg cttgatccgc
     3901 tgatggggct gttggccggt tctattacgc tttccggcgg tcacggtacg ggcgctgcgt
     3961 ggagtaaatt gttcattgaa cgttatggct tcaccaatgc gacggaagtg gcgatggcct
     4021 gtgcaacgtt cggtctggtg ctgggcggct tgattggcgg tccggtggcg cgctatctgg
     4081 tgaaacactc caccacgccg aacggtattc cggatgacca ggaagtcccg acggcgtttg
     4141 aaaagccgga tgtgggacgc atgatcacct cgttggtgct gattgaaact atcgcgctga
     4201 ttgctatctg cctgacggtg gggaaaattg ttgcgcaact tttggctggc actgcttttg
     4261 aactgccgac cttcgtctgt gtactgtttg ttggcgtgat tctgagcaac ggtctgtcaa
     4321 taatgggctt ttaccgcgtc tttgagcgtg cggtatccgt gctgggtaac gtaagcttgt
     4381 cgttgttcct ggcgatggcg ttgatggggc tgaaactgtg ggagctggct tcgctggcgc
     4441 tgccgatgct ggcgattctg gtggtacaga ccatcttcat ggcgttgtat gccatcttcg
     4501 ttacctggcg catgatgggc aaaaactacg atgcggcagt gctggctgcg ggtcactgtg
     4561 gttttggcct cggtgcaacg ccaacggcaa tcgccaacat gcaggcgatc actgaacgct
     4621 ttggcccgtc gcacatggcg tttttggtgg tgccgatggt cggtgcgttc tttatcgata
     4681 tcgtcaatgc gctggtaatt aagttgtatt tgatgttgcc gatttttgcc ggttaaccga
     4741 tgaagcggcg gtcttctcac cgccgccttt tacgcattcg agtaacgttc tgtctccggc
     4801 atccagcgtt ctatcaaggc ttttgcctgt tgtgggtaac gttcgtgaat atgtcgcgcc
     4861 aggcgctgaa cttccgggat catcgcctga tcgcgcagta aatccgccac tttaaattca
     4921 gcattacccg tctgacgcgt gcctaacaat tcgccagggc cgcgaatctc cagatctttt
     4981 tgcgcaatca caaaaccgtc gttactgtcg cgcagcactt gcaggcgaat ttgcgccgtt
     5041 ttagaaagcg gcgttttgta gagcagcacg cagtgagaag ccaccgcgcc acgacctacg
     5101 cgcccgcgca gctggtgtaa ctgcgccaga cccagacgct cagggttttc gataatcatc
     5161 aggctggcgt taggcacatc aacaccgact tcaataacgg tagtggcaac cagcagatgt
     5221 agctcacctt gtttaaacga cgccatcacc gcctgtttct cggctggttt catccgcccg
     5281 tgtaccaggc caacattcaa ttctggtagc gccagtttca actcttccca ggtagcttcc
     5341 gccgcctgcg cttccagtaa ctctgactct tcaatcaacg tacaaaccca gtacgcctga
     5401 cgaccttcag ttatgcaggc atggcgcacg cggtcaatga tgtcggtacg gcgggtatca
     5461 ggaatagcga ccgtagtcac tggcgtgcgg cctggcggca gctcatctat caccgaggta
     5521 tcgagatcgg catacgccgt cattgccagc gtgcggggga tcggcgtggc ggtcatgatc
     5581 aactgatgcg gatggaagcc ttgctgctgg cctttctccc ataacgccag acgctgatgc
     5641 acgccaaagc gatgttgttc gtcgataatc accagcgcca gaccgttaaa ctgcacctgt
     5701 tcctggaaga tggcgtgcgt accaacaatc atctgcacct gaccgctggc gatggcttcc
     5761 tgctgagaca gtcgcgcttt gcctttctgc ttaccggcga gccagcccac ttcgataccg
     5821 agcggttcaa accagttgcg gaagttattg gcgtgctgtt cggcgagtaa ttcggttggt
     5881 gccatcaatg ccacctgttt gccgtgggca atcgcacgca gcgcggcgag ggcggcgacc
     5941 agcgttttac cggaacctac atcgccctgt accaggcgca tcatcggcac atccagcgcc
     6001 atatcgcgct cgatctccgc cactacgcgt gcctgtgcgc ccgttggctt gaacggtaag
     6061 gctgcgagga gtttattttt cagcgcatca ttggcgctca gcggctgggc atgaaaacgc
     6121 tgtgctccgg cacgtaaggc taacatgctg aggttgtgcg ccagcagttc ttccagaatc
     6181 agacgacgtt gcgccggatg ctgcccggtt tccagatcgc taagctgtag cgtcggtggc
     6241 gggcggtgca aagtgcgcaa cgcttccggt agcgtcatca ttccttgtga cagttccggc
     6301 ggcaggagtt cttcaatggc gcaggtgtcg agcagatcca gcgcctggtc ggttaattta
     6361 cgcagcgtgg cctgctttac gccttccgtt gttggataaa ccggcgtgag cgtttcctgt
     6421 aattctggcg tgctgagatc gccctgcacg cggtattccg ggtggatcat ctccgcacca
     6481 tatttaccgc gctttgcttc gccataagcc agtacacggc ggcccgtcgc caggctattt
     6541 ttcattgccg cgctgaaatt gaaaaagcgc atggtgagga tgccggaacc gtcgctgatc
     6601 tggcaggtca tcatccgccg accgccgaag gagatattgc agttcagcac ttcgccttcc
     6661 accgtggcat aaacgcccgg cagtagttct ccgatggggt agagatgggt gcgatcttcg
     6721 tagcgcaggg gaaggtgtaa gagtagatcc tgcacggtat gcaggttgat tttcgccagt
     6781 ttgttactaa gtgctgcgcc aacgcccgtt agggaactga gtgggacagc atctaacagg
     6841 cgacctgtca tggcacttac cctgcagcct gcatagtagc ccaccagtcg gcatcagctt
     6901 cgatctcgcc ttgctgattg acgtggggat aaggcaggcc tttgcgtttt gcgactttcg
     6961 ccagcaccgg atagccgcct tcaaacaaca ggcgttgttg ctctgcttcc ggcaacatgc
     7021 tgttttcacg caggtacatg cctgcatttt gccgctgacg ctgggcttcg taaagaatga
     7081 gggctgaggc aacggaaaca ttcagcgact gcaccatgcc gatcatcgga atgatgatgt
     7141 cctgatccgc cagggccaat gcttcctgcg tgatgcccgt tttctcctgt cccatcaaaa
     7201 tgcaggtcgg gcgagtgtaa tcaatttcgc ggaaatcgac agcgttatca gaaagatggg
     7261 ttgccagaat ctgcatgccc tggcctttga gatgagcgac ggcatcgcca atggtgcggt
     7321 gtgttttcac ctgtacccag ctgttactac ccgccgctgc cgaagccatg gtgcgcatgc
     7381 ggctaccagg ccagacggcg tgaacttcat gtacgccaac ggcatctgcg gtacgaataa
     7441 tcgcagaaac gttatgaggt ttgtggacct gctccatgca gacggtcaga tcaggctgcc
     7501 gcctggcgag catttcgcag atgcgtgcat aacgtgttgg gttcataaaa cattaatttc
     7561 ggtttcgggt gactttaatc acgtctggca tcacgcggat tttgcgcatg atattcgcca
     7621 gatgcacacg gtcacgagcg gtcagacgaa taaaggcgct gtagacgcga ccatctttct
     7681 cttccgtatt caaactttga atattcgaag tcgtggtgtt aattgccgcc gtcaggtttg
     7741 ccagcgcacc ctgatgattg aacatctcca ccttgatttc ggtgatgaac tcctgcgccg
     7801 tctctttatc ccattccaca gccataaact tctctggctc tttctggtag ccacggatgt
     7861 tacggcagga ttcatggtgg atcaccagac ctttaccggg gctgacgtgg gcgataatcg
     7921 ggtcgccagg aatagggcgg cagcatttcg caaaggtgat cagcacgcca tccgcgcctt
     7981 tgatcggcag atgcccgtgg ctttgagtcg ccggtggaat ggaggcgtcc ccatgttgta
     8041 gatttttcgc gaccaccacg ctcattgcgt taccaaggcc gatttctgcc agcagatcgt
     8101 caagcgttgc cagcttcatg cgatccagct cgcgctgaat attttcctgc gggatttcat
     8161 tgagcttacg gctaccaccc aaagcatggt tgagcagacg acggcccagg cttacagaat
     8221 catcacgctt gaggtttttc agcaactgac gaattttggc gcgcgctttc gagctaacga
     8281 caaagttcag ccaagcggca ttcgggcgag cgcccggagc ggtaatgatt tcaacggtct
     8341 gaccgctagt aagcggctgc gacagcgggt aaggctggcg gtcgacgcgt gcgcccacgc
     8401 aggcatggcc gatatcggta tgcactgcat aagcgaagtc gacgggcgtt gcaccggcag
     8461 gcagctcgac aatgcgccct tccggtgtga aaacgtaaat ctcatccggg aagagatcgg
     8521 atttaacgct ctcgataaat tcaaacgaac taccggcgct ctgttgcagc tccagcaggc
     8581 tttgcatcca gcgctgggcg cgtatttgtg cggtagtact ggtttcgccg tgctctttat
     8641 aagcccagtg cgcggcaaca cccatctccg ccatctgatc catatcttcg gtacggatct
     8701 ggacctcaac cggcacgccg tgcgggccaa tcatcgaggt gtgcaacgac tgatagccgt
     8761 tcgcttttgg aatggcgata tagtctttca cgcggcccgg acgcggcttg tacaggctgt
     8821 gcatctggcc cagcacgcga taacaggtgt cagaatcatt gacgatcacg cggaaagcgt
     8881 agatgtccat gattgagtga aaacgctgct ctttgagcac cattttgcag taaatcgaat
     8941 aaagatgctt ttcgcgacca ctgacgcggc acggtattcc cgcttcctgc aaacgccctt
     9001 cgatttcaga aagaatcttc tggatcatct ctttacggtt gccgcgcgcg gctttcacca
     9061 cttctttgat tacgcgataa cggttgggat acagcgcctc aaaacccagc tcttcgagtt
     9121 cggttttaat gtggtggata cctaaacggt gcgccagcgg gctgtaaatt tcgagagttt
     9181 cacgggcgat gcggcgacgt ttgtccgggc gaagtgagcc cagcgtgcgc atgttgtggg
     9241 tacggtcggc aagtttgatg aggatgacgc ggatatcctg caccatcgcc ataatcatct
     9301 tgcgaaagtt ttcggcctgc gcctctttct tatcgcggaa cttgagttta tcaagtttcg
     9361 acaccccctc taccagctcg gcgacgcttt taccaaaaag ctgttccata tcctggtagg
     9421 tggcgggagt atcttcaatc acgtcatgca gcagcgccgc catcagcgtt tcatagtcga
     9481 gtttcatctc ggccagaatg caggcaaccg ctaccgggtg cgtgatatag ggttcaccgc
     9541 ttgaacgtgt ttgcccctcg tgagcatcac gtgcaacgag atacgcctgc cgcagacgct
     9601 tgatttggtc ttccggcagg taggtttgaa tcagttgatt caggctttca aacagataca
     9661 agggcgaccc gctttgtgat taacgacgac cttcagcaat agcggtaacg gcttgtaatt
     9721 cagcggcttc ctgctcttgc tgttcctggc gttcgcgaac gtcgaggatc tggttgttga
     9781 tcagaccttc ttcgatttcg cgcagcgcga ttacagtggt tttatcgttt tcttccggta
     9841 ccagcggatc ctttccgcct acctgcatct gacgagcgcg acgcgcggcg accagtacca
     9901 ggtcaaaacg gttaccaatt ttctctacag cgtcctgaac agttacgcgt gccatactta
     9961 aaaagctcca caggtgaaga aatgactggg catgatactg aaatcaggtt cagtctgcca
    10021 acaatttgct gattaaagcg tcatgacgct gcttttggcg gctcatgcgc agacgttcgg
    10081 cgcgaataat ggtcttcaaa tcggtcaacg cggtatcgaa gtcatcattc acaatcaggt
    10141 aatcatattc gtcgtaatgg ctcatttctg caacagcttg cgccatacgc tttgcaatga
    10201 cctcttcgct gtcctgaccg cgaccgcgta gacggcggtc cagttcaatt ttagacggcg
    10261 gtaaaataaa gatactccgc gcgtgcggca tcttctggcg aatttgctgc gcgccctgcc
    10321 agtcgatatc gagaaaaaca tcgacaccgg tcgccagtac ttgctcaatg gcctcacgcg
    10381 aagtgccata gtaattacca aaaacttctg cgtgttcgag gaacgcatct ctgctaatca
    10441 tttctttaaa ttcatcatga ttaacaaaga aataatgttc accgtggact tcaccaggac
    10501 gcggttggcg tgtggtgtgt gaaacagaaa cctgggtgtc atacaacggt tgggttttta
    10561 ataaagcctg aatcaggctg gatttacccg cgccactggg ggcagaaaca atataaagcg
    10621 tgccttgagc catgagtatc tttcgtatgt gattagcgaa ataaagccta catacgagct
    10681 tattatacac ggcgcggcca tgtgacgtag cccttgctca cactttttgc gcaaagttgt
    10741 tgcgttttgc gtagctgctt ccgcatttgc ctgtgattta ctgcaataaa gacgattctc
    10801 aggaagcgtc ctcgcagact ccgtttttgc cactcggctt tgccttcacc acgttgtata
    10861 tctgccagcc tgactacagc ggaggtgaag atgaaagtat ggatggcgat attaataagt
    10921 atcttgtgct ggcaatcatc tgtgtgggcg gtctgtccgg cctggtcgcc agccagagca
    10981 caggaagaaa ttttccgcca gcaacagcaa ataaaacagt gggacgatga ctactggaag
    11041 gaaggaaaaa gtgaggtgga agacggtgtt tacgatcagt taagcgcccg tcttacgcag
    11101 tggcaacgct gctttgtgag cgagccccgc gacgtgatga tgccgccttt aaatggtgcg
    11161 gttatgcatc cggttgccca tacgggtgta cgcaaaatgg cggataaaaa cgcattaagt
    11221 ctgtggatgc gagaacgtag cgatctttgg gtgcagccaa aagttgatgg cgttgcggta
    11281 accctggttt atcgggacgg gaaactgaac aaagcaatca gtcgcggtaa tggcctgaaa
    11341 ggcgaggact ggacgcagaa agttagctta atttccgctg tgccgcaaac cgttagcggg
    11401 cctttagcca acagtacgct tcagggggaa atatttctcc agcgcgaggg gcatatccaa
    11461 caacaaatgg ggggaataaa tgcccgcgca aaagttgctg gcttgatgat gcgccaggac
    11521 gatagcgaca cgctgaattc tctgggcgtt tttgtctggg catggccgga tggaccgcag
    11581 ttaatgtctg atcgtttaaa agagctggct accgcaggtt ttactctaac gcagacgtat
    11641 acccgtgcgg tgaaaaatgc tgatgaagtt gcgcgcgtac gcaatgagtg gtggaaagcg
    11701 gaattaccct tcgtcaccga tggcgtagtt gtacgagcgg cgaaagagcc agaatcccgc
    11761 cattggctac cgggccaggc agagtggctg gtggcctgga aatatcaacc tgtagctcag
    11821 gttgccgaag tgaaggcgat tcagtttgcg gtgggtaaga gcggtaaaat atcggtggtt
    11881 gcgtcactcg catctgtcat gctggatgat aaaaaagtcc agcgggtgaa tattggttcc
    11941 gtcaggcgct ggcaggagtg ggatattgcg cctggtgatc agattctcgt cagccttgcc
    12001 ggtcagggta ttcctcgcat tgatgatgtg gtgtggcgcg gtgcagaacg tacaaaaccg
    12061 acaccgccag aaaaccgctt taactcgttg acctgctact ttgcttctga tgtttgtcag
    12121 gaacagttca tttcacgctt agtcagtctg
//
LOCUS       DAJTYD010000116        12049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTYD010000116 DAJTYD010000000
VERSION     DAJTYD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.116"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..770)
                     /gene="ychF"
                     /locus_tag="OO585_002836"
     CDS             complement(<1..770)
                     /gene="ychF"
                     /locus_tag="OO585_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6630364.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFM"
     gene            complement(887..1471)
                     /gene="pth"
                     /locus_tag="OO585_002837"
     CDS             complement(887..1471)
                     /gene="pth"
                     /locus_tag="OO585_002837"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6630365.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            1749..2027
                     /gene="ychH"
                     /locus_tag="OO585_002838"
     CDS             1749..2027
                     /gene="ychH"
                     /locus_tag="OO585_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6630366.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2082..3761)
                     /gene="dauA"
                     /locus_tag="OO585_002839"
     CDS             complement(2082..3761)
                     /gene="dauA"
                     /locus_tag="OO585_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6630367.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(3886..4833)
                     /gene="prs"
                     /locus_tag="OO585_002840"
     CDS             complement(3886..4833)
                     /gene="prs"
                     /locus_tag="OO585_002840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6630368.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(4984..5835)
                     /gene="ispE"
                     /locus_tag="OO585_002841"
     CDS             complement(4984..5835)
                     /gene="ispE"
                     /locus_tag="OO585_002841"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6630369.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(5835..6458)
                     /gene="lolB"
                     /locus_tag="OO585_002842"
     CDS             complement(5835..6458)
                     /gene="lolB"
                     /locus_tag="OO585_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6630370.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6672..7928
                     /gene="hemA"
                     /locus_tag="OO585_002843"
     CDS             6672..7928
                     /gene="hemA"
                     /locus_tag="OO585_002843"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6630371.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            7970..9052
                     /gene="prfA"
                     /locus_tag="OO585_002844"
     CDS             7970..9052
                     /gene="prfA"
                     /locus_tag="OO585_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6630372.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9052..9885
                     /gene="prmC"
                     /locus_tag="OO585_002845"
     CDS             9052..9885
                     /gene="prmC"
                     /locus_tag="OO585_002845"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6630373.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            9882..10274
                     /locus_tag="OO585_002846"
     CDS             9882..10274
                     /locus_tag="OO585_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6630374.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10278..11087
                     /locus_tag="OO585_002847"
     CDS             10278..11087
                     /locus_tag="OO585_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6630375.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11123..11977
                     /gene="kdsA"
                     /locus_tag="OO585_002848"
     CDS             11123..11977
                     /gene="kdsA"
                     /locus_tag="OO585_002848"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6630376.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3009 a         3052 c         3151 g         2837 t
ORIGIN      
        1 tgcataaact cgtcacgttc ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca
       61 caaaccggaa ccacaacaga accttctttc gccgcgattt cacgcacctg gtcaagatat
      121 gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt acattgttgg tttcagcgtc
      181 aggaagctca ggtaacgaat agccgctttc tcttcagcgc ttaaatccag cgcgcgcagc
      241 atacctgcat tttccaactg gggcaggcat ttttccagga ccgccagctc agctttcgcg
      301 tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc gcaggtgtcg
      361 aggtctgcca gcgccagttc ggtgttgata acttcaatat cgtcagccgg gttaactttg
      421 ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc gatcgcttcg
      481 gtttcacgga tgttggtcag gaactggtta cccagacctt cgcctttcga tgcgcctttt
      541 accagaccgg cgatatcgac aaattccatg gtcgtgggaa gcgtacgctg cggttttacg
      601 atttcagcca gttgatccag gcgaggatca ggcattggta cgacgcctgt gttcggctca
      661 atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc gttgaacagg
      721 gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcccat gatttaaatc
      781 accttaatat cttaataatc aacctgttat tgctaacaga ttgcagaaat ggaaataact
      841 ttgcctatta tacacggcac tcggcaaaaa tgccgcagac aacgacttat tgcgctttaa
      901 aggcgtgcaa tcggttcgtt gctttggtca agccatctgt aaaccacatt tcagtacaac
      961 gcgccgcttc gtcaatggct tcatcaatta acttctgttc actaacaggc ggtttgccta
     1021 acacaaaacc gacaacttta tttttatcgc ccggatgacc gattccgatg cgtaaacggt
     1081 gaaagttagg gttattaccc aatttactga tgatgtcttt cagtccattg tgaccaccat
     1141 ggccaccgcc caatttaaat ttggcgacgc caggaggcag atccagttcg tcgtgggcca
     1201 ccagaatttc gtccggatta atgcggaaaa aactggccat cgccgcaacg gctttgccgc
     1261 tgagattcat aaatgtagtc gggactaaca ggcggacatc ttcgcctcca agagtgactc
     1321 gcgaagtata accaaagaat ttagcctctt cgcgcagcgg agcgcgcaaa cgctctgcca
     1381 gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc ggcgtattca gcaccggggt
     1441 tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg tcctgagtgt gtacataact
     1501 ggcgcgtagt ttactggttg cgaccccgct tgacaaaaaa ctgcgtatca aatgcagata
     1561 acgtaataat tgcctgagtg ggctattaga aagtcaaggt gttcaggcgt ttatttgtaa
     1621 agttttgttg aaataagggt tgtaattgtg atcacgcccg cacataaccc acagggtgtt
     1681 gtctatactt tacacataag gaagaggggt attccctgtt acaacccaga aagttccgga
     1741 ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa cgtgctcatg gggttgggtc
     1801 tggtggtaat ggtggtcggc gtggggtatt caatcctcaa ccagttacca cagtttaata
     1861 tgccccagta tttcgcacat ggtgcagtgc taagtatttt cgtcggtgcc attctctggc
     1921 tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga ccgttactgg tgggttcgcc
     1981 actatgacaa acgttgccgc cgtagcgata atcgccgtca tagctaacaa ataatgcagt
     2041 ttgctgacca gtcagtttgc gctgactggt caatctcgta cttataaatc cgccatcgcc
     2101 gcgcgacgat tcgggaagaa cgcgaggcgt cccgagattg gttgaatgcc tgcgcgagcc
     2161 atagtgcgca gtggctggaa ttccacgttg cacacgcgca gttcacatcc ttcgggcaga
     2221 cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat caagtaccgg aacggcatcc
     2281 cacttcagaa tcacaatccg tttgccttca agacgtgact ccaggtccgt gaacaagcct
     2341 tcagcagcag caaaaaacag cgggccaata acgcgcagta ccagaacatc gtctggaaca
     2401 tctacgacta ccggtgccag gcgagtcata cgtgcgatac gacgcataaa cagcagcgat
     2461 gccagcacga tccccacgct gatggcaata accatatcaa acagcacggt cagcgacatg
     2521 cacagcagca tgacaatgat gtcatctttc ggtgcatgac gcagcaagtc gaccacttta
     2581 tgcgcttcac tcatgttcca cgccaccatc aacagcaggg ctgccatagc ggaaagcggc
     2641 agccaggaga gcagcggtgc cagtaccagc agggcaagaa taaccagaat agagtggatc
     2701 accgctgaga taggggaagt tgccccggca cggacgttag cggcagaacg cgcgatggca
     2761 gctgtagcgg taataccacc aaagaatgga gcgataatat tccccagtcc ctgtccaacc
     2821 agttcgctgt tcgccttgtg tttcgtcccg gtcataccat ccagtaccac ggcgcagagc
     2881 agagattcga ttgcgccgag cattgccatt gagaatgccg caggcagcag tgtgcgaata
     2941 gaatcccagg ttagcgtgaa ttctgaatta ggcagatccc acggcagcac cagttgcggc
     3001 agcagttgcg gaataccgtt accctgagaa ccatcggcca ggacgtagtg gaattgcgaa
     3061 ccgatggtag caacatgtcc gccgagcagg ttaacaatcc ccatcaccgc gcaaccagcc
     3121 agcaaggccg gaaggtgacc gggtaaacga atgcccagac gcggccaaaa aacaagaata
     3181 cctagcgtca caatgccaat ggcagcatca cccacattaa tggtcggcag cgccataaat
     3241 aatgcgccga ctttttgtag ataatgttcc gggacatggg ccatttgcag accgagaaaa
     3301 tctttaatct gcatggtacc gatggtgatc ccgatacccg aggtgaaacc taaggtgacg
     3361 gaaaccggaa tatactcaat caggcgacca aagcgtgcca gacccatcag aatcaaaaag
     3421 atccccgaca gcaaggccgc aaccagcagt cctgccagtc caaactgttg cgacacggga
     3481 tagataatta ccacaaatgc cgcagtcgga ccggaaacgc taaagcgtga cccacccgtc
     3541 agagcaatga caatccccgc aacagctgcg gtatataaac cgtactgggg tgccacacca
     3601 ctaccaatag ccaacgccat cgccagcggg atagcaataa tcccgacggt tatcccggca
     3661 atcaggtcac gggtaaaccg tgcggcagta tatttttctt tccagcaagc gtcgatcaga
     3721 gcgcggaaag gcatcacatg tgaggaaaat attttgttca caataatgtt tcatccgtga
     3781 gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat aggtcataca aatggatatt
     3841 acagacaaaa aaacccgccg cagcgggtct ttgagccggg ttcgattagt gttcgaacat
     3901 ggcagagatc gattcttcgt tgctgatacg acgaatcgct tcggccagca tacctgacag
     3961 ggtcagagta cgcacgttcg gcagtgattt gatttcatcg ctcagcggaa tggtatcgca
     4021 gacaacgact tcatcaatta cagagttacg caggttgttc gccgcgttgc cagagaagat
     4081 cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt tctttcagag cttcagcagc
     4141 tttacacagc gtaccgccag tgtcgatcat atcatcgacc agtacgcagt cacgacctgc
     4201 aacgtcaccg atgatatgca tcacctggga aacgttcgca cgcggacgac gtttgtcgat
     4261 gattgccata tcggtatcgt tcagcagctt agcgatagcg cgggcacgca caacgccgcc
     4321 gatgtccgga gaaaccacaa ttgggttatc cagattcagc tgcagcatgt cttccagcag
     4381 gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag aaaccctgaa tctgttcagc
     4441 gtgcagatcc actgtcagca cacggtcaac accgacgctg gagaggaagt ctgcaaccac
     4501 tttcgcagtg attggtacac gagcggaacg gacgcgacgg tcctggcgcg catagccaaa
     4561 gtaggggata acagcggtga tacgacctgc ggaagcacga cgcagggcat caaccataac
     4621 gactaattcc atcaggttgt cgttagtagg ggcacaagtg gactggatga tgaaaatatc
     4681 accaccgcgt acattttcat taatttgtac gctgacttcg ccatcgctaa agcgacctac
     4741 agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata cgttgtgcta gttccggggt
     4801 ggcgttacca gcaaaaagct tcatatcagg cacgagaaga acctcaggca tgcgtccatt
     4861 ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg atcctttcca ggcagtgtat
     4921 taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt caccgaaact cagcttgccc
     4981 ggcttaaagc atggctctgt gcaatgggga aagattaacg cctttcgcca caaagccatt
     5041 gagccattcc ggggcttgct ctagcacctg gcgggcttca gactctgtat caaattcagc
     5101 aaagacacag gcccctgtcc cagtcaggcg cgacggggcg tattctaaca gccaggaaag
     5161 caccgcatca acctcgcgaa aacgttttct tgcgataacc tcgcaatcat tgctgaattc
     5221 acattttagc aacgtttcta ttgacctttt tggcgtattg cgcgggagtt caggatcttt
     5281 aaaaatcacc ggagtcggaa tacttacacc agggtgcgcc accagatacc acttctctgg
     5341 cggatccacc ggcgttagta tttcaccaac gccttcggca aacgcggcat gcccccgaac
     5401 aaagacagga acatctgcgc ccagcgtcag ccccatttcc gccagctcat ccatgcttag
     5461 cccgcattgc cagagatgat ttaatgccac caggaccgtc gcggcattgg atgaaccacc
     5521 gccgagaccg ccgcccatcg gcaaacgctt gtcaatgctg atattcgcac cgcttcccgt
     5581 cggaagacgc ccgctgtctg ccgcagtttt catcaacaat cgcgctgcgc gaacgatcag
     5641 gttatcttca tgttccacgc cttcaacggg cgttaacaga cgaatatccc catcgtcacg
     5701 aagctcaatg ctgatggtgt cgccgtaatc aagaaactga aacagcgttt gcagcgtgtg
     5761 gtaaccatcc gcacgctgac cggtaatgta taaaaacaga ttaagttttg ccggagaggg
     5821 ccactgtgtc cgcattattt cactatccag ttatccattt ttaacttgat gcgttgacca
     5881 ccgtcggtga gttccatatt ggctggcatc gcaggatgcg ttttggtgtc ataaccacca
     5941 taaacaacct tccagttttt gccattctgg ctgtaggtaa tttcgctcag gcggtactgg
     6001 tcgtccagtt tgtagtcggt tgcatcaccc ggtaaaccta aaatccactg gcgcaagctg
     6061 ttgagcggaa ttggcattcc ggtcaatttg ccaatcatct cttcggcgtc atcggcggta
     6121 taacgctgac ctttattgtc gactaactgc acgttacccg gttgagcatt cagctccagt
     6181 tccgtgctgc ccaatgggtt agtgagcagc agacggtagc gatcctggcc ggtttgctgc
     6241 cagaaaaagc gggcgtacac tttttgttgg tcagaaatat aagcgaacgc gccgcgagtc
     6301 tgatactgat taagattgcg cacgtcttgc tgatgctgac gccattgtgg cgaatccggg
     6361 cttttgccag gacctttggg cgtggtaacg gaacaggcag tgagcacaag agcagccagc
     6421 ggtagcaggc ggataagacg aaaatcgggc aggggcatag tgatgacaag tccttgagat
     6481 acgttgcagt tataaccctt aatgctagcg ttaccgtccg ctatcgtcta tgttcaagtt
     6541 gtcttaattg ccagaatcta acggctttcg gcaattactc caaaaggggg cgctctcttt
     6601 tattgatctt acgcatcctg tatgatgcaa gcagactaac tctatcaacg ttggtattat
     6661 ttcccgcaga catgaccctt ttagcactcg gtatcaacca taaaacggca cctgtatcgc
     6721 tgcgagaacg tgtatcgttt tcgccggata agctcgatca ggcgcttgac agcctgcttg
     6781 cgcagccgat ggtgcagggc ggcgtggtgc tgtcgacgtg caaccgcacg gaactttatc
     6841 ttagcgttga agagcaggat aacctgcaag aggcgttaat ccgctggctt tgcgattatc
     6901 acaatcttaa tgaagaagat ctgcgtaaaa gcctctactg gcatcaggat aacgacgcgg
     6961 ttagccattt aatgcgtgtt gccagcggcc tggattcact ggttcttggg gagccgcaga
     7021 tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa aggtcatatg aaggccagcg
     7081 aactggaacg catgttccag aaatctttct ctgtagcgaa acgcgttcgc actgaaacag
     7141 atatcggtgc cagtgctgtg tctgtcgctt ttgcggcttg tacgctggcg cggcagatct
     7201 ttgaatcgct ctctacggtc acagtgttgc tggtaggcgc gggcgaaacc atcgagctgg
     7261 tagcgcgtca tctgcgcgaa cataaagtac agaagatgat tatcgccaac cgcactcgcg
     7321 aacgtgccca aattctggca gatgaagttg gcgcggaagt gattgccctg agtgagatcg
     7381 acgaacgtct gcgcgaagcc gatatcatca tcagttccac cgccagcccg ttaccgatta
     7441 tcgggaaagg catggtggag cgcgcattaa aaagtcgtcg taaccaacca atgctgttgg
     7501 tggatattgc cgttccgcgc gatgttgagc cggaagttgg caaactggcg aatgcttatc
     7561 tttatagtgt ggacgatctg caaagcatca tttcgcacaa cctggcgcag cgtaaagccg
     7621 cagcggttga ggcggaaact attgtcgctc aggaaaccag cgaatttatg gcgtggctgc
     7681 gagcacaaag cgccagcgaa accattcgcg agtatcgcag ccaggcagag cacgttcgcg
     7741 atgagttaac cgccaaagcg ttagcggccc ttgagcaggg cggcgacgcg caagccatta
     7801 tgcaggatct ggcatggaaa ctgactaacc gcttgatcca tgcgccaacg aaatcacttc
     7861 aacaggccgc ccgtgacggg gataacgaac gcctgaatat tctgcgcgac agcctcgggc
     7921 tggagtagca gtacatcatt ttcttttttt acagggtgca tttacgccta tgaagccttc
     7981 tatcgttgcc aaactggaag ccctgcatga acgccatgaa gaagttcagg cgttgctggg
     8041 tgacgcgcaa actatcgccg accaggaacg ttttcgcgca ttatcacgcg aatatgcgca
     8101 gttaagtgat gtttcgcgct gttttaccga ctggcaacag gttcaggaag atatcgaaac
     8161 cgcacagatg atgctcgatg atcctgaaat gcgtgagatg gcgcaggatg aactgcgcaa
     8221 agctaaagaa aaaagcgagc aactggaaca gcaattacag gttctgttac tgccaaaaga
     8281 tcctgatgac gaacgtaacg ccttcctcga agtccgagcc ggaaccggcg gcgacgaagc
     8341 ggcgctgttc gcaggcgatc tgttccgtat gtacagccgt tacgccgaag cccgccgctg
     8401 gcgggtagaa atcatgagcg ccagcgaggg tgaacatggt ggttataaag agatcatcgc
     8461 caaaattagc ggtgatggtg tgtatggtcg tctgaaattt gaatctggcg gtcatcgcgt
     8521 gcagcgtgtt cctgctacgg aatcgcaggg tcgtattcat acttctgctt gtaccgttgc
     8581 ggtaatgcca gaactgcctg acgcagaact gccggacatc aacccagcag atttacgcat
     8641 tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt aacaccaccg attcggcaat
     8701 tcgtattact cacttgccga ccgggattgt tgttgaatgt caggatgaac gttcacaaca
     8761 taaaaacaaa gcgaaggcgc tttcagtgct cggtgctcgc atccacgctg ctgaaatggc
     8821 aaaacgccaa caggccgaag cgtctacccg tcgtaacctg ctggggagtg gcgatcgcag
     8881 cgaccgtaac cgtacttaca atttcccgca ggggcgcgtt accgatcacc gcatcaacct
     8941 gacgctctac cgcctggatg aagtgatgga aggtaagctg gatatgctga ttgaaccgat
     9001 tatccaggaa catcaggccg accaactggc ggcgttgtcc gagcaggaat aatggaatat
     9061 caacactggt tacgtgaagc aataagccaa cttcaggcga gcgaaagccc gcggcgtgat
     9121 gctgaaatcc tgctggagca tgttaccggc agagggcgta cttttattct cgcctttggt
     9181 gaaacgcagc tgactgacga acaatgtcag caacttgatg cgctactgac acgtcgtcgc
     9241 gatggtgaac ccattgctca tttaaccggg gtgcgagaat tctggtcgtt gccgttattt
     9301 gtttcgccag cgaccttaat tccgcgcccg gatacggagt gtctggtgga gcaggcactg
     9361 gcgcggttgc ctgaacaacc ttgccgtatt cttgatctcg ggacgggtac cggggcgatt
     9421 gcgcttgcgc tggctagcga gcgcccggac tgcgaaatta tcgctgtaga tcgtatgcct
     9481 gatgctgtct ccctggcaca acgtaatgcc cagcatctgg cgatcaaaaa tatccacatt
     9541 ctgcaaagcg actggtttag cgcgctagcc gggcagcagt ttgcgatgat tgtcagcaat
     9601 ccgccgtata ttgacgagca ggacccacat cttcaacaag gcgatgtccg ctttgagccg
     9661 ctcactgcgc tggttgcggc agacagtgga atggcagaca tcgtgcatat catcgaacag
     9721 tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg aacatggctg gcagcagggc
     9781 gaagcggtgc gacaagcatt tatcctcgcg gggtatcatg acgtcgaaac ctgccgtgac
     9841 tatggtgata acgagcgcgt aacgctcggc cgctattatc aatgacaagt ttttctacac
     9901 tgcttagtgt tcatcttatt agtatcgcgc tttctgttgg gctattaacc ttacgtttct
     9961 ggctacgtta tcagaagcat ccacaggcat ttgctcgctg gacgcgcatt gtgccgccgg
    10021 ttgtcgatac ggtgttattg ttaagcggca ttgcgttgat ggctaaagcg cacatcctgc
    10081 cattttccgg gcaggcacag tggctgactg aaaagctgtt tggagttatc atttatatcg
    10141 ttttgggttt tattgcactc gattatcgtc gtatgcacag tcagcaggcg cgcattattg
    10201 ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa actcgccacc acaaaagtac
    10261 cgttactggg gtaagtcatg agatcgttag ctgatttcga atttaataaa gcgccattgt
    10321 gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga ttttccctcg caagatgttt
    10381 acgacgaact ggagcgtctc gttagtctgg cgaaggaaga aatcagccag cttctgcctt
    10441 tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg cgactgggga tttaaagcct
    10501 cacgcggcgt ttatcgtctt tccgatgcat tatggctgga ccaggtgtta aagaatcgac
    10561 agggcagtgc ggtatcatta ggtgcggttt tattatgggt cgcgaatcgt ctcgatttgc
    10621 cgctgctgcc ggtgattttc cctacgcagc tgatattgcg cattgaatgt ccggatggcg
    10681 aaatttggct gattaatcct tttaacggtg aatcgttaag cgaacatatg ctggacgtat
    10741 ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta tgaagacctt gatgaagctg
    10801 ataacattga ggtaatccgc aaattgctgg atacactcaa agcctcgttg atggaagaaa
    10861 atcagatgga gctggcgtta cgcaccagcg aagctttatt acaattcaac cctgaagatc
    10921 cctatgaaat tcgcgatcgc gggttgattt atgcgcaact ggattgcgaa cacgttgcgt
    10981 tgaacgattt aagttatttc gttgaacagt gtccggaaga cccgatcagc gaaatgatcc
    11041 gtgcgcaaat aaataacatc gcgcataaac atattgtgct gcattaatta atcgacattt
    11101 tactcaagat taaggcgatc ctatgaaaca aaaagtggtt agcattggcg acatcaacgt
    11161 agcaaatgac ctgccgttcg tactgtttgg cggtatgaac gtgctggaat ctcgcgatct
    11221 ggcgatgcgc atttgcgagc actacgtaac tgtgacccag aaactgggta tcccttacgt
    11281 gttcaaagcc tcttttgaca aagccaaccg ctcctccatc cactcttatc gtggaccggg
    11341 cctggaagaa gggatgaaaa tcttccaaga gttgaagcag acttttggcg tgaaaattat
    11401 caccgacgtt cacgaaccaa gtcaggcaca gcccgttgct gatgtcgtgg atgtgattca
    11461 gttgccggcg tttcttgctc gccagactga cctggttgaa gccatggcga aaaccggtgc
    11521 ggtaattaac gtcaagaaac cacagtttgt cagcccggga cagatgggta atatcgttga
    11581 taaattcaaa gaaggcggca acgaaaaagt gattctttgc gatcgtggtg ctaacttcgg
    11641 ctatgacaac ctggttgtcg atatgctggg cttcagcatt atgaagaaag tgtctggtaa
    11701 ctcgccggtg attttcgacg tgacccacgc actgcaatgc cgcgatccgt ttggcgcagc
    11761 ttccggtggt cgtcgtgctc aggtggctga gctggcacga gccggtatgg cggtaggtct
    11821 ggcggggctg tttattgaag cgcatccgga tccggaacat gcgaaatgtg atggtccatc
    11881 cgcgctgccg ctggctaaac tggaaccgtt cctcaagcag atgaaagcga ttgatgatct
    11941 ggtgaaaggt ttcgaagaac tggataccag caagtaatct tttttgcttg aaaaataaag
    12001 tcttagcgtt ctgcgttaag acttttttca tgggtgccag atacaaaaa
//
LOCUS       DAJTYD010000117        12046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTYD010000117 DAJTYD010000000
VERSION     DAJTYD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.117"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..582
                     /locus_tag="OO585_002849"
     CDS             <1..582
                     /locus_tag="OO585_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin"
                     /protein_id="HCR6630377.1"
                     /translation="AQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEK
                     EAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAV
                     IEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPL
                     IDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            821..880
                     /locus_tag="OO585_002850"
     CDS             821..880
                     /locus_tag="OO585_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6630378.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1197..1256
                     /gene="ibsE"
                     /locus_tag="OO585_002851"
     CDS             1197..1256
                     /gene="ibsE"
                     /locus_tag="OO585_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6630379.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1376..2809)
                     /gene="hldE"
                     /locus_tag="OO585_002852"
     CDS             complement(1376..2809)
                     /gene="hldE"
                     /locus_tag="OO585_002852"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6630380.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(2857..5697)
                     /gene="glnE"
                     /locus_tag="OO585_002853"
     CDS             complement(2857..5697)
                     /gene="glnE"
                     /locus_tag="OO585_002853"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6630381.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(5720..7021)
                     /gene="ygiF"
                     /locus_tag="OO585_002854"
     CDS             complement(5720..7021)
                     /gene="ygiF"
                     /locus_tag="OO585_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6630382.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7263..7883
                     /locus_tag="OO585_002855"
     CDS             7263..7883
                     /locus_tag="OO585_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6630383.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            7947..9185
                     /gene="cca"
                     /locus_tag="OO585_002856"
     CDS             7947..9185
                     /gene="cca"
                     /locus_tag="OO585_002856"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6630384.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9342..10163)
                     /gene="bacA"
                     /locus_tag="OO585_002857"
     CDS             complement(9342..10163)
                     /gene="bacA"
                     /locus_tag="OO585_002857"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6630385.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10254..10622)
                     /gene="folB"
                     /locus_tag="OO585_002858"
     CDS             complement(10254..10622)
                     /gene="folB"
                     /locus_tag="OO585_002858"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6630386.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10727..11344
                     /gene="plsY"
                     /locus_tag="OO585_002859"
     CDS             10727..11344
                     /gene="plsY"
                     /locus_tag="OO585_002859"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6630387.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11357..>12046)
                     /gene="ttdR"
                     /locus_tag="OO585_002860"
                     /gene_synonym="dan"
     CDS             complement(11357..>12046)
                     /gene="ttdR"
                     /locus_tag="OO585_002860"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR6630388.1"
                     /translation="RLVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPEL
                     QVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQ
                     SLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGI
                     MLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLVAWCQQR
                     LGKPDEGYQVM"
BASE COUNT         2865 a         3446 c         3003 g         2732 t
ORIGIN      
        1 gcgcaacaaa acgtagaaac cactcgccag accgcagaag ccgatcgtgc taaacaagtt
       61 gccctaatcg ctgccgcgca ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg
      121 aaagcagaaa aagaagccgc agagatgcag gcggcggcta tcgttaagtt agccgaagct
      181 acacgtaaaa agggtctggc ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac
      241 gtactttctg atgaacaaac cagccttaaa ttcaaactgg ccttgttgca ggcgctacct
      301 gcggtaatag aaaaatccgt tgagccgatg aaatctattg acggtatcaa gattattcag
      361 gtcgatggtc tgaatcgtgg cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc
      421 ggaaacctgg cggaacaagc attatcagcc gctctctctt accgcacaca ggcaccgctg
      481 attgactcct tgctcaatga aattggcgtt tcaggcggct cactggcggc attgacttca
      541 cccttaacct caacaactcc cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt
      601 ccctggcaca aaaagccggg gattttttat atctgcgttc cgctaaaaac tgcaaatgat
      661 caggtcgttg caacgttttg cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc
      721 gttgctcgag gtccggcgct catcaacaat cggggagtca gcaaagggct gaaacgggaa
      781 agcccctccc gaggaagggg ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat
      841 actgattgtg ttgttactcg taagtttcgc agcttattaa cagccaatca gaggggggag
      901 aaatcctccc tcacccttgt tcctttactt taggttgaaa aaaaatcagc gtcaagaagg
      961 tttcattaac acatatccct agcccaaaaa gccggggatt ttttatatct gcgttccgct
     1021 aaaaggtgca aatgctcagg acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa
     1081 ctgcttcagg gaaagcgttg cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1141 ggggctgaaa cgggaaagcc cctcccgagg aaggggcctt atagaaggaa agggttatga
     1201 tgaagttcgt catcatactg gttgtgttgt tactgttaag tttcccgact tactaacaac
     1261 tgatcagagg ggggagaaat cctcccttac ccttgttcct ttactttagg ttgaaaaaac
     1321 aacagcgtca ataggcctgc ctgctacgaa gcgagatctg tgaaccgctt tccggttagc
     1381 cttttttatc ctgttggatc ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt
     1441 tgagcaccag cacttcgccg ccgttggccc agacttcttt actcccggcg atctcttctg
     1501 gtttatagtc gccgcccttc accagtaaat ctggcaggat cccggcgatc aagcgctgcg
     1561 gcgtgtcttc ttcaaacgac accacccagt cgaccgcttc cagtgcgccc agcacaatca
     1621 tacgctgttc gagcgggtta acggggcggg aatccccttt cagccgtttg gtggaggcat
     1681 cgctgttcac agcgacaatc agacggtcac ccagtttgcg agcatttgcc agataggaga
     1741 cgtgcccggc atgcaggatg tcaaagacac cgttggtcat taccaccttt tcgccgcgtt
     1801 tacgcgctgc cgctacggcc agcttcagtt cctcttcggt catcacgcca aagccggtat
     1861 ctgcgcgccc acgcactgca ttttccagct cgatcggcga aaccgtggag gttcccagtt
     1921 tgccgaccac cacgccagcc gccgcattgg caaagaagca ggcttcttcc agcgaattac
     1981 ccgctgccag cgttgccgcc aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat
     2041 acacttcctg cgcctgggtt ggcatatgca gcggcgcttt acccggttgc agcagcgaca
     2101 taccctgttc ggaacgggtc actaacagag ccgagagttc gtaatcggca atcagtttca
     2161 tgccgcgctc aacaatctct tcttcggtct tacatttacc gacaacagct tcaaattccg
     2221 agagattcgg cgttaacagc gtagcgccgc ggtagcgctc aaaatcggta ccttttggat
     2281 caatcagcac cggaacgccc gctttacgcg ccagttggat catctgctgt acgcttgcca
     2341 gcgcaccttt ggcgtagtca gaaagcacca gcgcgccaat cgaactcagc gcctgattaa
     2401 tccgctcgtg cagcggctgc ggatcaacgc cttcgaaacc ttcttcgaaa tccagacgga
     2461 tcagctgttg gttgcgggaa agtacccgta atttggtgat ggtcggatgc gtcggtacag
     2521 aaacgaagtc gcatttgacg ttgacgtcgg ccagagattt actcagcgcg cgcgctgcat
     2581 cgtcaatgcc cgtcaacccg accaggcgtg cattagcacc gagagaagcg atattcatcg
     2641 ccacgttagc cgcgccgccc ggacgttctt cgatggtatt cactttaacc acgggcaccg
     2701 gcgcttccgg cgagatacgg ctggtgggac cgtaccagta acgatccagc attacatcac
     2761 caaccaccat cactcctgca cgttcaaact ctgacagcgt tactttcatt cctgtctcct
     2821 gagagattca aaatttgcgc gcgataatac catacttcat tcttccacca gccacttctg
     2881 ccagcttgcc cgcaccagat cacgctctgc ggtgaagcaa tcctccgaca catggcccgg
     2941 caattcctgt aatgccagat gatgaagttc atcgcgcagc gtcgtgtaag cacgggtcag
     3001 cgccatcgct tcctgctctt ccataatgtc gttttgcgcc agcagctcca gaatacgcac
     3061 gttgtctgac cagcgcgtta acttcggttt ttcatgagcg tagcgcaaca ccagatattg
     3121 ggtaataaat tcgatatcgg taattccgcc ttcatcggct ttgatatcaa agcgattacg
     3181 atgtttattg ccgagatgag cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag
     3241 agttttacct tcacgcggca gcatcataat ctcgcgacgc actgcgtcaa agtgcgcggt
     3301 gagctgcgga tcgccgtaca ctacacgcgc acgcaccagc gcctgatgct cccacgtcca
     3361 ggcctcgttt ttctgataat cagcaaatgc ttctgcggat gtcaccagca ttcccgccgc
     3421 cccggacgga cgcagtcgtg catccacttc atacaaaatg ccggaagagg ttcgcgtatt
     3481 gaacagatgc ataatgcgtt gcgccagacg cagataaaac tgtcgcccgt cgatttcccg
     3541 ctcgccgtca gtcatcgcat ccattgggca atcatggagg aagatcaggt caagatcgga
     3601 actgtagcct aactcccagc cgcccagctt gccgtagccg accaccgcaa aaccacggcc
     3661 ttcgcgatcg ttgaggtgat ttggcttacc gtagcgggcc accatttgaa cccacgcctg
     3721 ctgaacgacg gcatctatca tagcctctgc cagccaggtt aagtgatcgc tcactttcat
     3781 caccggtagc gtaccagcga tatccgctgc ggcgatgcgt aacagctgcg cctgtttgaa
     3841 ctgacgcagc gcctcaagct gttgctcttc gtcatcttcc ggcacgcgca gcaaatactg
     3901 gcgcaactca tcgcggtagg catcggtcgc cgtcggctgg taaagagtgt ttggatcgag
     3961 caattcatcc agcaataatg gataacgcgc cagctggctg gcaatcatcg gcgatgcggc
     4021 acacagagaa atcaaatgtt taagcgccgc agggaattca ctgagcaatt ctaaataggt
     4081 ggtgcgggta acaatcccca ccagcaaggc ggtaatgcgc gacagcgtaa cggcagcgtc
     4141 ttcacgcgca cagacatcac ttagcagatg tggcatcaga tggtcgagca cctgacgtcc
     4201 tcgcggcccg atagtacgct tatccagctc tttgcggaaa tcagcaatca gcgttaggac
     4261 ctgtttgcga tcatcctcgc taagatgcgc cagcactggc gtagtgtcat cttcctgcaa
     4321 cgcatcctgc cacagctcac gccactgttc cgacagcgac tcttcctgag tttcactttc
     4381 gtcgtcgcca atcaattcat taaacacccg gcgcacattg gtcatatgtg cggtcagcgc
     4441 cccggtcagt tgcggccagt cagcaaagtc catcgcccac gccagccgcg cacggttaag
     4501 ctcatcagaa ggaagcgttt gggtttgttc gtcgttaatg ctttgcagca ggttttccag
     4561 acgccgcagg aacagatatg ccactcgcaa ttgttcagca tcgttttcag aaagcagatg
     4621 cagcgcggca atgacgctga gcgttggtag taaagagcgc gactgcagcg acggttcgcg
     4681 cccaccgcga atgagctgga acacctgaac gataaattca atttcgcgaa tgccgcctgc
     4741 gccgagtttg atattgtcgg tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt
     4801 catgttgcgc agcgactgaa tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg
     4861 cagcatcgca cgcaactcgt tagcatagac gccttcgcta tcgcccataa tccgcgcctt
     4921 gaccatcgcg taacgctccc agtcgcgccc ctgctcctgg taataatcct ccaacgcggc
     4981 aaagctcagc accagcgggc cactttcgcc aaacggacgc agccgcatat ccacgcgata
     5041 gacgaagcca tcctgcgttg gttgatccaa cactttaatc agccgctgcc ccatgcgggt
     5101 aaaaaactgc gcgttatcca gttcccggcg tccgccctgc gtacaaccat gttccggcca
     5161 ggcaaaaatc agatcgatat ctgaggagaa attcagttcc ccaccgccca gcttacccat
     5221 gcctaaaatc agcagcggtt gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg
     5281 gcagcaagcg tcatacagcc agtcacgcgc ggcgacaatc agcgtctccg ccagatggct
     5341 gagctgctgc aatatactct cttcagtaac caaagccagc gtttgcgccc aggcgatgcg
     5401 caccataatg cgccgccgga atagccgcag ctcgcgcatt aacccggctt cgtcactcac
     5461 attgctgagc gcctcctgca accatgccgc gtaatgctgc cattcatcag cctgcggcga
     5521 ttggctttcc agttccgtca gccactctgg atgcgcaatc acgctgtcct gcacaaaatc
     5581 actgaaagta agtactgact tcgcctgtgc gctaagtgat ttctcggcta aaggctctgg
     5641 cagccgctca acaacggtct gccagtactg ctgtaacggt gaagagagcg gcttcataag
     5701 cgattttatc cttgcctgat taacgttttc cgctgtgcaa ccagaacggt tcctgattgt
     5761 ttgcctcatt acggaaatgt tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca
     5821 gcccctgcca gttctccagc caggcttgcg cgacgacagg atcatagtaa cccgccagca
     5881 acagtattga gtcaatatca cgcgtcagac gtggcaactg gtcacggtag cgatcgccca
     5941 acggctggca gaaaacgctt ttcagttcag cggcatggcg ggaaagatgg atatcggcaa
     6001 agcgtttgaa ggaatcgctc attttactct gcgctttggc atctaaaaac ggctgccagg
     6061 ctttgcttac cagccattcg gtcaacgcca gcttcgccat tgccgttttg gtagagtaga
     6121 ccgccgtcac ggcagaaacc gcagaagcga tggtcgcttc gcattgagtc agcaaatcgc
     6181 gtaagtgagt gctcgctttg cgcggcacaa taccaccgaa cagcatcagt gtatgacgga
     6241 ccagaccaat ggctgccagc acctgttctt tcgccgcatc gttgccgcgt acccacagtt
     6301 cttcatgata ctgccattgc gctaacgcca gctcgagcgc cgcttccagc ccctgttcca
     6361 catcggcttt tgccgcaaca tgcaaaatgg tggtcggttt gatttcacgc gccggattgc
     6421 cctgcgccag atgatagcca cgcgccgctt tgctcaggct gccctggcgt aatccggttt
     6481 gcgataccag ttggttcgcc agtttcagca ccgcgcgcgt gtcgccgcta agcagttcca
     6541 gttccagctc acagataggt tcagcaaatt cccccgcttt cacctccccc tgatcgagag
     6601 cgatttcaat tcggctgtca tcgaccgcca ccagccattt ttcgcgataa aaatcggtgc
     6661 tgaacagcgg ttgtacgcgg gaagcgagat cggcgggcaa ttcgccgttc ggccagactt
     6721 ccgtcggtaa ctgcgccagg tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc
     6781 gctggtgtaa gccgcctgtc actcttcctg caactttcat ggtcatctca tagcgaccgt
     6841 tttcgccacg aatacgtaag cccatatcgt gcccacgcag ccagttatcc ggcgtttcgt
     6901 agtaaatatt cagcaactgc acggggtcat ggtgctcgcc gcccagcgta ttgagatggt
     6961 cacgcaacgc ctcaacggca ctgtgattaa caataaactt taattcgatt tcctgagcca
     7021 tggcctttac ttatcggtta tgtcacataa gtgacgatga acggcgaact taatgcgatc
     7081 ttttttgtca gtagatagta ttttgcgcca aattgccatg caacgggcaa tttgacgggc
     7141 gtaaaagttt gaagcagtgg caagacctga cacagaggat tccgattgat gtcgaatgct
     7201 ttgcgtagcg acactgatgc cactactatc gttccacttt ccatgacaat aacgacagcc
     7261 tgatgccaaa attacgcctg atcggattaa ctttactcgc acttagcgcc actgccgtct
     7321 cacacgctga agaaacgcgc tatgtttctg atgaactgaa tacctgggtc cgtagcggtc
     7381 cgggagatca ttatcgcctc gtgggcacgg ttaacgccgg cgaggaagtg accttattac
     7441 aaactgacgc caacaccaat tatgcccagg tgaaagacag ctctggccgt accgcctgga
     7501 tcccgttgaa acaacttagc actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa
     7561 atcaggtcaa aaccctgacc gataaactca ccaatatcga taacacctgg aatcagcgca
     7621 ccgcagaaat gcagcaaaaa gtggcgcaga gcgacagcgt gatcaacgga ttaaaagaag
     7681 aaaatcagaa attgaaaaac gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg
     7741 tacagctgga tgacaaacag cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg
     7801 tgctggggct tggcttgctg ctcggtctgg tactgccaca cctgatccca acgcgcaaac
     7861 gcaaagagcg ctggatgaac taaatcgcct tcttcgttgc actgtcttat tattaggaat
     7921 tgtgtatttg acgaagggat gatggcgtga agatttatct ggtcggtggt gctgttcggg
     7981 atgcattgtt agggctaccg gtcaaagaca gagattgggt ggtggtcggc agtacgccac
     8041 aggagatgct cgacgcgggc taccagcagg taggccgcga ttttcctgtg tttctgcatc
     8101 cgcaaacgca tgaagagtat gcgctggcac gtaccgaacg gaaatccggt tccggttaca
     8161 ccggttttac ttgctatgcc gcaccggatg tcacgctgga agatgatctt aagcgtcgcg
     8221 atctgaccat taatgcgctg gcccaggacg ataacggcga gattatcgac ccgtacaacg
     8281 gtctgggcga tctgcaaaat cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc
     8341 cgttacgcgt tctgcgcgtg gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta
     8401 ttgccgatga aactctgacg ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc
     8461 tgacgcctga acgggtatgg aaagagacgg aaagcgccct caccacccgc aatccccagg
     8521 tgttcttcca ggtactgcgc gattgcggtg cacttcgcgt tttattcccg gaaattgacg
     8581 ccctgtttgg cgttccggcc cctgccaggt ggcatccgga aatcgacacc ggtattcata
     8641 ccttaatgac gctctcaatg gcggcgatgc tgagtccgca ggttgatgtc cgtttcgcaa
     8701 ctttatgcca cgatctcggt aaaggactga cgccgccaga actttggccg cgtcatcatg
     8761 ggcatggccc ggcgggtgtt aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa
     8821 atgaaattcg cgatttagcc agactggtgg ctgagtttca cgatctcatc cacaccttcc
     8881 caatgctgaa cccaaaaacc atcgtcaaat tatttgattc catcgatgcc tggcgtaaac
     8941 cgcagcgtgt cgagcaactg gcgctgacca gcgaggctga cgtacgcggc agaaccggtt
     9001 ttgaatcagc ggactacccg caaggccgtt ggttgcgcga agcctgggaa gtggcgcagt
     9061 cagtgccgac aaaagcggtc gttgaagcgg gatttaaagg tgtggagatt cgcgaggagc
     9121 tgacccgacg gcggattgcg gcggtagcca gttggaagga acaacgttgc ccaaagcctg
     9181 actgagtata aaactaaacg ttaacacgaa ccatcccctc gccccttcgg ggagagggtg
     9241 aggggaacag accagcaccg gtgcgaacaa tcacccccac cccggccctc tccctacaga
     9301 gcgagggaga aaaccaggcc tgctcccgaa ccttttctca atcaaaagaa cacgacgtac
     9361 accgcagccg ccacaataaa gcgataaatg gcgaacggga taaacgaaat gcgcttaatc
     9421 aattgcagga aggttttaat cgctatcagc gccaccacaa aagcggtgat aaacccaacg
     9481 gcaaacatcg ggatatcgcc ggttgtcagg aagccccagc ttttgtagag atcgagcgcc
     9541 gttgcgccca tcatcatcgg taccgccagc aggaacgaga actcggaagc agcgtaacgg
     9601 ctcacgccca tcagcatccc acctgaaatg gtggccccgg aacgggagaa acccggccac
     9661 agcgccagac actggaaaca gccaatcata aacgcctgac gataggtcat atcatcaaga
     9721 cccggcgcac gcggctcttt cggcttcagg cattcggcgg caatcagcaa caaaccgcca
     9781 acgaccagtg catacatcac atttatcggg ttaaacaatg acttaatcgt gtcgtggaac
     9841 aacagcccca gtaccaccgc cggaatcatc cccagcaaaa tgtggatcag cgttaaacga
     9901 cctttgcttt ctccttcgtg ctgcaacggg cggccaaagt ggatgccaat caggccaaac
     9961 agacgccgcc agaacatcac tactaccgcc agaattgatc ctaactggat cacaacttca
    10021 aaggttttcg ccgtgtcgcc ctcgaacccc aacaagtgac cgacaataat catatggccc
    10081 gtgctggata ccggcagaaa ttctgtcaat ccttcgacca cacccaatat tgccgctatc
    10141 agcagcgagt gcatatcgct catcaataaa cccctaaatt attaaaatgt accgcttgtc
    10201 cggactactg cagtaagacc aggttataac cgtttggttt aacagctgta aaattaatta
    10261 ttttctttca gattattgcc acgctcaatg attacaccaa cattcgccgc ccgcgccact
    10321 gcgcctggct tgctgagttt gatgcgcacc cacggcgagt tgaagcgtgc taacagcagc
    10381 tccgccacct cttcagccac gcgttccacc agcgcaaaac gcgccccctc gacgtggctg
    10441 accaccgttt ctgcaatgtc agcgtaactg aggcaatccg ccacatcatc acttttcgcc
    10501 gctttacggt tatcccatgc catttcgata tcgaacacta acttctgttc gatggtctgt
    10561 tcccagtcgt aaacaccaat agtggtgatt accgaaagtt gctctataaa tacaatatcc
    10621 atcacgtcct gcctgctttt tggctaaccg gataccactt ccggcgaaat gtgcgtatta
    10681 tccacagatt catcgttgaa cacgaatttt caaaacggaa cagcttatga gtgcaatcgc
    10741 gcctggaatg atcctcatcg cgtacctctg cggctccatt tccagtgcca ttctggtttg
    10801 ccgcttgtgt gggctgcccg atccgcgaac cagcggctcc ggcaatccag gcgcaaccaa
    10861 tgtgttacgt atcggtggca agggagcagc cgtagcagta ctgattttcg acgttctgaa
    10921 aggaatgttg cccgtctggg gcgcgtatga attaggtgtc agccccttct ggctaggctt
    10981 aattgccatc gccgcctgtc ttggacacat ctggcccgtt ttcttcggat ttaaaggagg
    11041 aaaaggcgtt gctaccgctt ttggtgccat cgcacccatt ggctgggatc tcaccggagt
    11101 aatggcggga acctggttac tgaccgtgct attgagcgga tactcgtcgc tgggagcgat
    11161 tgtcagtgca ctgattgctc cgttttatgt ctggtggttt aagccacaat tcaccttccc
    11221 agtttcgatg ctctcttgcc tgatcctgct gcgtcatcat gacaacatcc agcgtctgtg
    11281 gcgtcgtcag gagacaaaaa tctggacgaa attcaaaaga aagcgcgaaa aggatcccga
    11341 gtgatttctg gtggatctac atgacctgat agccttcatc gggcttgccc agccgttgct
    11401 ggcaccatgc caccagaaat tccacgcaga cacgtaattt catgctgcga tagagcggct
    11461 cccggtaaac agcccagata ttggcgcttt gtgcatactc cggcaatact tgcaccagtt
    11521 tgccactctc cagaaacggc aacacatccc actcggaacg cagcataatc cctttgccct
    11581 ccagcgccca ttgcaacact atctcgccgc tattggagga aagatgcccg ctaaccttca
    11641 ccgatttttt ctcctgaccg ttccccaact cccatattcc gtgggtcata tcgcgctctt
    11701 tggtcaccag acagtcatga cgacttaatt cctgtaatga ttgtggctgc ggatattttt
    11761 gcagatactc aggtgctgcg cataatattc ttttattttt cgttaacaga tgcgcaatat
    11821 aataatcagg aatttcgtca ttaatacgaa tatccagatc aatattatcc tgcactaaat
    11881 caatttgccg atcgaacaat tcaaaatgca cctgtaactc aggataattg cgcataagtt
    11941 cggtaatggc tggcgcaata tggctgcgcc caaaaccaaa actgcaacca atacgaatca
    12001 tcccctccgg gcgcgttttg atttgcgtga cgtcatcgac cagtcg
//
LOCUS       DAJTYD010000118        12004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTYD010000118 DAJTYD010000000
VERSION     DAJTYD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.118"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..456
                     /gene="mepH"
                     /locus_tag="OO585_002861"
     CDS             <1..456
                     /gene="mepH"
                     /locus_tag="OO585_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6630389.1"
                     /translation="VKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGGSSPR
                     TGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQGRGTA
                     DHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            584..1165
                     /gene="sodB"
                     /locus_tag="OO585_002862"
     CDS             584..1165
                     /gene="sodB"
                     /locus_tag="OO585_002862"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6630390.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1311..2480)
                     /locus_tag="OO585_002863"
     CDS             complement(1311..2480)
                     /locus_tag="OO585_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6630391.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(2646..2735)
                     /gene="ynhF"
                     /locus_tag="OO585_002864"
     CDS             complement(2646..2735)
                     /gene="ynhF"
                     /locus_tag="OO585_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6630392.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3034..4059
                     /gene="purR"
                     /locus_tag="OO585_002865"
     CDS             3034..4059
                     /gene="purR"
                     /locus_tag="OO585_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6630393.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4056..4988)
                     /locus_tag="OO585_002866"
     CDS             complement(4056..4988)
                     /locus_tag="OO585_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6630394.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5101..6312
                     /locus_tag="OO585_002867"
     CDS             5101..6312
                     /locus_tag="OO585_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6630395.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            6603..7751
                     /gene="cfa"
                     /locus_tag="OO585_002868"
     CDS             6603..7751
                     /gene="cfa"
                     /locus_tag="OO585_002868"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6630396.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(7791..8432)
                     /gene="ribE"
                     /locus_tag="OO585_002869"
     CDS             complement(7791..8432)
                     /gene="ribE"
                     /locus_tag="OO585_002869"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6630397.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            8647..10020
                     /gene="mdtK"
                     /locus_tag="OO585_002870"
     CDS             8647..10020
                     /gene="mdtK"
                     /locus_tag="OO585_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6630398.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10061..11317)
                     /locus_tag="OO585_002871"
     CDS             complement(10061..11317)
                     /locus_tag="OO585_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6630399.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            11625..11701
                     /locus_tag="OO585_002872"
     tRNA            11625..11701
                     /locus_tag="OO585_002872"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11659..11661,aa:Val,seq:gac)
     gene            11706..11782
                     /locus_tag="OO585_002873"
     tRNA            11706..11782
                     /locus_tag="OO585_002873"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11740..11742,aa:Val,seq:gac)
     gene            11890..>12004
                     /locus_tag="OO585_002874"
     CDS             11890..>12004
                     /locus_tag="OO585_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HCR6630400.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLW"
BASE COUNT         2858 a         3132 c         2900 g         3114 t
ORIGIN      
        1 gtcaaaaatg ccgcgtcagg aactcttgcc gacgcgcaca aagcgaaggt gcaaaaagct
       61 acgaaagtgg caatgaataa actgatgcag caaattggta agccatatcg ttggggtggc
      121 agctcaccgc gtaccggttt tgattgcagc ggcctggttt attacgctta taaagatttg
      181 gtgaaaattc gtattccgcg tacggcgaat gaaatgtatc acctgcgtga tgcagcgcca
      241 atcgaacgta gtgaactgaa aaacggcgac ctggtctttt tccgtactca gggacgcggc
      301 acagccgatc atgtcggcgt gtatgtcggc aacggcaaat ttattcagtc accgcgcaca
      361 ggtcaggaaa ttcaaatcac ctctctcagt gaagactact ggcagcgcca ctatgttggc
      421 gctcgtcggg taatgacccc aaaaacactt cgctaaaact ttaccctgtt gttacggcaa
      481 cagggtaagt tcatcttttg tctcaccttt taatttgcta ccctatccat acgcacaata
      541 aggctattgt acgtatgcaa attaataata aaggagagta gcaatgtcat tcgaattacc
      601 tgcactacca tatgctaaag atgctctggc accgcacatt tctgcggaaa ctatcgagta
      661 tcactacggc aagcatcatc agacttatgt cactaacctg aacaacctga ttaaaggtac
      721 cgcgtttgaa ggtaaatcac tggaagagat tattcgcagc tctgaaggtg gcgtattcaa
      781 caacgcagct caggtctgga accatacttt ctactggaac tgcctggcac cgaacgccgg
      841 tggcgaaccg actggaaaag tcgctgaagc tatcgccgca tcttttggca gctttgccga
      901 tttcaaagcg cagtttactg atgcagcgat caaaaacttt ggttctggct ggacctggct
      961 ggtgaaaaac agcgatggca aactggctat cgtttcaacc tctaacgcgg gtactccgct
     1021 gaccaccgat gcgactccgc tgctgaccgt tgatgtctgg gaacacgctt attacatcga
     1081 ctatcgcaat gcacgtcctg gctatctgga gcacttctgg gcgctggtga actgggaatt
     1141 cgtagcgaaa aatctcgctg cataataact gatggcaaat gcagcattgc ctgatgcgct
     1201 tcgtttacca tgccaacgcg gatgaggcgt taacagcgca ttcggcaaga taaatcgcac
     1261 gttgtcagca actgtaacgc agaaggttat ccttctgcgt ttttgtttaa ttagctgtta
     1321 gcaacgcaaa ctgtttcagg ttgttttctg gctgacataa acaccagcaa taatgccagt
     1381 cccgcgacaa tcgctcccat caccggcaca aagctgtatc ccagcccagc ggaaattacc
     1441 gcaccaccag cagctgctcc cagcgcattt ccaagattaa aggcaccaat attgactgat
     1501 gaagacagac ctggcgcttc actggcgaca cgcatcacgc gcatctgtaa cggcggtacg
     1561 accgcaaagg ctgctgcgcc ccacaccacc atgctaatag ctgcgccgaa ctcattgcgg
     1621 gccaggaacg ggattgccag cataatcacc atcaacaaca acaaaaagcc tttcaacgtg
     1681 ccgttaactg aacgatctgc cagtttgccg ccgggatagt taccgataga gaatccgaca
     1741 ccaatcagca ccagcattgc cgtgacgaac accggtgttg cgtgggtaat actttgcagt
     1801 accggagaga tataggtgta gagagtaaac attgcaccag ctcccagtac cgtcgtcagc
     1861 aatgcagaca acacctgcgg acgcattaat accgccagct cttttttcac ttcaggtcgt
     1921 gcccctgcac cacctttagg taatgagaag aacagactta ccattgaaat cactcccagc
     1981 cccgccgttg ccagaaatga catccgccag ccgatggttt cacccaacca ggtcgccgcc
     2041 ggcacgccac cgatatttgc cagggttaac cccataaaca tagtggcaac tgcgctggcc
     2101 tgtttatgtt ttggctccac gcttgcggcc acgactgaac ccaaaccaaa aaatgctccg
     2161 tgattcaggc tggtcaaaat gcgtgaaagc atcagggtca tataatccgg cgcgatggcg
     2221 gaaagtacgt tgccgagcgt gaaaattgcc atcaggaaaa tcaacgcact gcggcgggca
     2281 cgatgagaaa gtagaagcgt catcagcggc gcgccaacca ttacgccaac tgcataggca
     2341 ctgattaaca ttccggcagc gggaatcgag acatccacac cgcgcgcaat gacgggcaac
     2401 aagcccattg gcgagaactc cgttgtcccg ataccaaacg cgccaatcgc cagagccagc
     2461 aacggatagt taattttcat gccttatctc cacctcttcg cgtcattacg cgatattcat
     2521 taaagtggcg aaagcatgac agcaatcaca aaaaaatgaa aataacaaaa agagaaaaca
     2581 cttttgccat tttgctaaca aacaggaagg agatgcgagg gagaacgcgc tccctcgaga
     2641 ggaaatcagt gcagcgcggc agtcaaaccc acggccacga tcaaaccgag gacgataatc
     2701 gttgttacca gtgaaaattt aaggtcggtg ctcatcaagt tttctccttt tttattacca
     2761 cacaaatagt gatattacgc atttttacac actgtgatga aaaaatctcc cgtcatttat
     2821 aatgataagt gtttttacca cttccccttt tcggcaagat cggccaaaat tccacgctta
     2881 cactatttgc gtactggcca ttgacccctt cctgacgctc cgtgtcgttt ttccggcgta
     2941 ccgcaacact tttgttgtgc gtaaggcgtg taaaggcaaa cgtttacctt gcgattttgc
     3001 aggagctgaa gttagggtct ggagtgaaat ggaatggcaa caataaaaga tgtagcgaaa
     3061 cgagcaaacg tttccactac aactgtgtca cacgtaatca acaaaacacg tttcgtcgct
     3121 gaagaaacgc gcaacgccgt gtgggcagcg attaaagaat tacactactc ccctagcgcg
     3181 gtggcgcgta gcctgaaggt taaccacacc aagtctatcg gtttgctggc gaccagcagc
     3241 gaagcggcct attttgccga gatcattgaa gcggttgaaa aaaattgctt ccagaaaggt
     3301 tacaccctga ttctgggtaa tgcgtggaac aatcttgaga aacagcgggc ttatctgtcg
     3361 atgatggcgc aaaaacgcgt cgatggtctg ctggtgatgt gttctgagta cccagagccg
     3421 ttgctggcga tgctggaaga gtatcgccat atcccaatgg tggtgatgga ctggggtgaa
     3481 gcaaaagctg acttcaccga tgcggtcatt gataacgcgt tcgaaggcgg ctacatggcc
     3541 gggcgttatc tgattgaacg cggtcaccgc gaaatcggcg tcatccccgg cccgctggaa
     3601 cgtaacaccg gcgcaggccg ccttgccggt tttatgaagg cgatggaaga agcgatgatc
     3661 aaggtgccgg aaagctggat tgttcagggt gactttgaac ccgaatctgg ttatcgcgcc
     3721 atgcagcaaa tactgtcgca gccgcatcgc cctactgccg tcttctgtgg tggcgatatc
     3781 atggcaatgg gcgcactttg tgctgctgat gaaatgggtc tgcgcgtccc gcaggatgtt
     3841 tcgctgatcg gttatgataa cgtgcgcaac gcccgctatt ttacgccggc gctgaccacg
     3901 atccaccagc caaaagattc gctgggtgaa acagcgttca acatgctgtt ggatcgtatc
     3961 gtcaacaaac gtgaagaacc gcagtccatt gaagtgcatc cgcgcttgat tgaacgccgc
     4021 tccgtggctg acggcccgtt ccgcgactat cgtcgttaat cacccgttgc gggagtctct
     4081 tccggctccc gcagccactc cttattcagc gtctcactat cgccgagata ctcaagcagc
     4141 caggttaacg caggcgacat atcattttgc tgccatgtca gacaacatgc cgaatccggg
     4201 aaggggtttt ccaattcaag tgctacccac ttcccctcat taagccacgg tttggcgaaa
     4261 tgtgttggca ccatccctat gcataatcct gccgagatac aggttgccga tgattcccag
     4321 tcaggcacga caactctttt ttggttatcc agtaaccagg taatacgttt aggtagcgtt
     4381 cgcgaggtgt cttcgcgcac caacgacggc cagttgcgca acgtatcatc gctgaatggg
     4441 ccatccatca acgccagcgg gtggtggctg gcaacaacgc aactccagct tagcatcccc
     4501 atatcccgga aggcataacg accgcctacc ggaatcgcgc gtgttgcgcc aatcgccagt
     4561 tccacgcgcc cgtcggaaag cgcatcccag acaccgttga acacttcctg aaagacaaga
     4621 agttcgacat catcaaaatg gcgataaaaa tcaacgatca tctgccgtgt acgttctggc
     4681 ctgacaatat tatccactgc gatagctaac tgaccgcgcc agccgttcgc tatctgctga
     4741 cattgctggc gggtgatctg catttttttg acaacagagc gcccttcttt gagaaaccac
     4801 gctccagcag cggtcagctc cacatcacgg tgccgtcgtt caaagagcgg caccgccagc
     4861 cactcttcca gctgacgcac ggtatagctg accgcagaag gaacgcgatg cagctcctgt
     4921 gccgcagcgc taaaactacc attacgcgct accgcatcaa caacttcgag tgaatattct
     4981 gaccacatag tctgcctgca aaatttttga aatcagtcat caaatattac cgtttcacaa
     5041 cactaatttc actccctaca ctttgcggcg gtgtttaatt gagagattta gagaatatac
     5101 atgcaacctg ggaaaagatt tttagtctgg ctggcgggtt tgagcgtact cggttttctg
     5161 gcaaccgata tgtatctgcc tgctttcgcc gccatacagg ccgacctgca aacgcctgcg
     5221 tctgctgtca gtgccagcct tagtctgttc cttgccggtt ttgccgcagc ccagcttctg
     5281 tgggggccac tctccgaccg ttatggtcgt aaaccggtgt tattaatcgg cctgacaatt
     5341 tttgcgttag gtagtctggg gatgctgtgg gtagaaaacg ccgctacgct gctggtattg
     5401 cgttttgtac aggctgtggg tgtctgcgcc gcggcggtta tctggcaagc gttagtgacg
     5461 gattattatc cttcacagaa agttaaccga atttttgcga ccatcatgcc gctggtgggt
     5521 ctatctccgg ccctggctcc tctgttagga agctggctgc tggtccattt ttcctggcag
     5581 gcgattttcg ccaccctgtt tgccattacc gtggtgctga ttctgcctat tttctggctc
     5641 aaacccacga cgaaggccgg taacaatagt caggatggtc tgacctttac cgacctgcta
     5701 cgttctaaaa cctatcgcgg caacgtgctg atatacgcgg cctgttcagc cagttttttt
     5761 gcatggctga ccggttcacc gttcatcctt agtgaaatgg gctacagccc ggcagttatt
     5821 ggtttaagtt atgtcccgca aactatcgcg tttctgattg gtggttatgg ctgtcgcgcc
     5881 gcgctgcaga aatggcaagg caagcagtta ttaccgtggt tgctggtgct gtttgctgtc
     5941 agcgtcattg cgacctgggc tgcgggcttc attagccatg tgtcgctggt cgaaatcctg
     6001 atcccattct gtgtgatggc gattgccaat ggcgcgatct accctattgt tgtcgcccag
     6061 gcgctgcgtc ccttcccaca cgcaactggt cgcgccgcag cgttgcagaa cactctacaa
     6121 ctgggtctgt gcttcctcgc aagtctggta gtttcctggc ttattagtat cagcacgcca
     6181 ttgctcacca ccaccagcgt gatgttatca acagtagtgc tggtcgcgct gggttacatg
     6241 atgcaacgtt gtgaagaagc tggctgccag aatcatggca atgccgaagt cgctcatagc
     6301 gaatcacact gatctatatc gatatactta tacttaggct gctaacaaaa ttttgttgta
     6361 tgattgaaat tagcggccta tactaatttc gagttgttaa agctacgata aatattatgt
     6421 ttttacgggg acaggatcgt tcccgactca ctatggatgg tcatttcggc aagggttcct
     6481 cctttccctc tgttctacgt cggattatag actcgcggtt ttttctgcga gatttctcac
     6541 aaagcccaaa aagcgtctac gctgttttaa ggttctgatc accgaccagt gatggagaaa
     6601 ctatgagttc atcgtgtata gaagaagtca gcgtaccgga tgacaactgg taccgtatcg
     6661 ccaacgaatt acttagccgt gccggtatag ccattaacgg ttctgccccg gcggatattc
     6721 gtgtgaaaaa ccccgatttt tttaaacgcg ttctgcaaga aggctctttg gggttaggcg
     6781 aaagttatat ggatggctgg tgggaatgtg accgactgga tatgtttttt agcaaagtct
     6841 tacgcgcagg tctcgagaac caactccccc atcatttcaa agacacgctg cgtattgccg
     6901 gcgctcgtct cttcaatctg cagagtaaaa aacgtgcctg gatagtcggc aaagagcatt
     6961 acgatttggg taatgacttg ttcagccgca tgcttgatcc cttcatgcaa tattcctgtg
     7021 cttactggaa agatgccgat aatctggaat ctgcccagca ggcgaagctc aaaatgattt
     7081 gtgaaaaatt gcagttaaaa ccagggatgc gcgtactgga tattggctgc ggctggggcg
     7141 gactggcaca ctacatggca tctaattatg acgtaagcat ggtgggcgtc accatttctg
     7201 ccgaacagca aaaaatggct caggaacgct gtgaaggcct ggatgtcacc attttgctgc
     7261 aagattatcg tgacctgaac gaccagtttg atcgtattgt ttctgtgggg atgttcgagc
     7321 acgtcggacc gaaaaattac gatacctatt ttgcggtggt ggatcgtaat ttgaaaccgg
     7381 aaggcatatt cctgctccat actatcggtt cgaaaaaaac cgatctgaat gttgatccct
     7441 ggattaataa atatattttt ccgaacggtt gcctgccctc tgtacgccag attgctcagt
     7501 ccagcgaacc ccactttgtg atggaagact ggcataactt cggtgctgat tacgatacta
     7561 cgttgatggc gtggtatgaa cgattcctcg ccgcatggcc agaaattgcg gataactaca
     7621 gtgaacgctt taaacggatg tttacctatt atctgaatgc ctgcgcaggt gctttccgcg
     7681 cccgtgatat tcagctctgg caggtcgtgt tctcacgcgg tgttgaaaac ggccttcgag
     7741 tcgctcgcta aaggctattc tatcgccccc tctccggggg cgatttcaga tcaggcttct
     7801 gtacctggtt gattcatggc attttctcgt gccgccagca cacgttctac cgtatctacc
     7861 actgcctgag tttgtggatc aatttcaatg ttgacgcgtg cgccgagttt tttcttccca
     7921 agagtcgtgc gttccagagt ttccggaatt aaatggacgc aaaaacgcgt tggcgtgact
     7981 tcgccgacgg tcaggctaat accgtcgatg ccaataaatc ctttgtacag gatatatttc
     8041 atcaactgac tatcctggac tttaaaccag atctggcgat tattttccga ggttaatatt
     8101 ttcgccactt cagcggtggt cataatatga cctgacatta agtgtccgcc aatttcatca
     8161 ctgaatttcg ccgcacgctc aacgtttacc caatccccca cttttaaatc gccaagattg
     8221 gtaatgcgta acgtttcttt catcaggtca aaactgacat ggttgccgtt aatttccgtc
     8281 acggtcaggc agcaaccgtt atgcgccacg gaagcaccgg tttccaggcc gtccagcatg
     8341 tggtcgggta actccaccac atgcgtacga aaatttggtt tctcgtcaat cgacaccagt
     8401 tttgcggtgc cctgtacaat ccccgtaaac atacttacaa ctcctgaaat cagttaagac
     8461 attctgttca gcacaatagc aggtggcaaa cgcccttacc agtgaagggg taagaatgac
     8521 tattttttca ctggagaatt aataaatcct cgctacaata gactgaattt cccctgcttc
     8581 ttctttttgc tgcccattca ggcggctttt tagtctctca tataactaca aataaaaggt
     8641 gttcacgtgc agaagtatat cagtgaagcg cgtctgttat tagcattagc aatcccggtg
     8701 attctcgcgc aaatcgccca aactgcgatg ggttttgtcg ataccgtgat ggcgggcggc
     8761 tatagtgcca ccgacatggc ggcggtcgct atcggtactt ctatctggct tccggcgatc
     8821 ctctttggtc acggactgct gctggcatta acgccggtta tcgcgcaatt aaatggttcc
     8881 ggtcgacgtg agcgcattgc gcatcaggtg cgacaaggtt tctggctggc aggttttgtt
     8941 tccgttctca ttatgctggt gctgtggaat gcaggttaca ttatccgctc catggaaaac
     9001 atcgatccgg ctctggcgga caaagccgtg ggttatctgc gtgcgttgtt gtggggcgcg
     9061 ccgggctatc tgttcttcca ggttgcccgt aaccaatgtg aaggtctggc aaaaaccaag
     9121 ccgggtatgg taatgggctt tatcggcctg ctggtgaaca ttccggtgaa ctatatcttt
     9181 atttatggtc atttcggtat gcctgagctc ggtggcgttg gttgtggcgt ggctactgcg
     9241 gcggtgtatt gggtcatgtt ccttgccatg gtttcttaca ttaaacgcgc ccgctccatg
     9301 cgcgatattc gtaacgaaaa aggcaccgcg aaacccgatc ctgcggttat gaaacgactg
     9361 attcaactcg gtttaccgat tgcgctggca ctattctttg aagtgacact gtttgccgtc
     9421 gtggctctgt tagtgtctcc gctcggtatt gttgatgtcg caggacacca gattgccctg
     9481 aactttagtt cactgatgtt tgtgcttcca atgtcgctgg cggcagcggt aactatccgc
     9541 gtaggttatc gtctgggtca gggctcaacg ctggatgcgc aaaccgctgc gcggaccggg
     9601 cttatggtgg gtgtctgtat ggcaaccctg acggccattt tcacggtttc actccgggag
     9661 caaatcgccc tgttgtacaa cgacaatccc gaggttgtaa cgctggctgc gcatttgatg
     9721 ttgctggcgg cggtatatca gatttctgac tcaatccagg tgattggcag tgggattttg
     9781 cgtggttata aagatacgcg ttccattttc tatatcacct ttacagctta ctgggtgctg
     9841 ggcttgccaa gcggctatat tctggcactg accgatctgg tcgttgaacc tatggggcca
     9901 gcaggcttct ggataggctt tattatcggc ctgacatcgg cagccattat gatgatgttg
     9961 cgtatgcggt tcctgcaacg tctgccgtca gttatcattc tgcaacgagc atcccgctaa
    10021 taaagacaag gcgcaacctt cacgggttgc gcctgtgttt ttacgcaggc tggagcgttg
    10081 cgccaatccc gtcttcgtct ggctgtaatt tcagagcgtt gcacagagtt tgccgactgg
    10141 catctgttat ctcaacgtgt atatcaccgg taacttcccc tttcacattg atgaagtcag
    10201 gtgagatttc tttcgcactg gtttgcaggc aaatcgctcc ctctccgcgc aggcgtttca
    10261 ctttcagttg cacatgcgtt gccgcaccag tgatcagcca cagttttccg ccattcagat
    10321 tgatgttacc aacaataaaa gtgacaccgt caccttttac cgtactgccg tcgagaatca
    10381 acacaccgcc ctgctgtaca gtaatcgttg attgttgcag atctgcgcca tagccaataa
    10441 tcattgaagc gccgtccagt aatgcaacac tcccggtcag ttcaaccggt gcgcgatctt
    10501 cctctacgac aaaattttcg tccgcgctgg tgggtgacaa caggatcact gttccacctt
    10561 gtattaccgc attttccagc gtaccggcat gcacttcagc ccgaccaccc acattcacgg
    10621 acaggttttg cgtcttaccg gaactgtaga gctgaaggcc atccgtcccc agacgataaa
    10681 tggatccatt ctcaacaatg gtgtcagagt cttcaccacc gagattattc atggtgccac
    10741 cagcaagcaa cgtatcactg gctttcccgg ccagcataaa aacgccgccc tgattaatct
    10801 cacaattgct ggcttcccca cctggataca ccaacaattc accaccttca tctaccacga
    10861 ccgctgaggt tgtcccatta accaacaggc cgccctcttg atcgagaatg attttttccg
    10921 cgcgatgtcc ttccagtaca cgcaggttac cgccattttc cagcaacaaa ccgcaggcat
    10981 aaccctgatc aacgctgaat tcaccttcgg gatggcggcc attaaccgta gcgagcgtag
    11041 agagactaat tgcgcctccc tcggactgaa caatatctgt ggcaatggca ccgtcgctga
    11101 ccgatagcgt gccattgtta ccgtactgtg tgcatttggc gatcccaccg gttgccactt
    11161 tcaaccagcc agcccccgtg actacggtat tttcggctct tccgccagct tcaacaatca
    11221 actcgccttc agtaagttgc gtcgcgccaa tgacctcgcc ggtcttaaca atttgcacat
    11281 tcccgtcgct catcaagttt ttcgcatcag atcccatgat ttattccttt gctgcatctg
    11341 tgtgccttta ttgctaccta aatgtaaagg ctacggagga tttatccacg acagatttga
    11401 gatggtggca aacaactctg tttaaactct gatacacgaa ttattgggtt gtatcagatg
    11461 taaatgcgat cctgaataaa aatcgccctt gcaaatcaac aaaatatagc caatttgctt
    11521 aaaagctctg caaacggtcg aaatagcgca gaaaattacg ttttgcctct tgccaccttc
    11581 ccactctgcc gctaatattc gtccccgttg tcacctacaa cgttgcgttc atagctcagt
    11641 tggttagagc accaccttga catggtgggg gtcgttggtt cgagtccaat tgaacgcacc
    11701 atcctgcgtc cgtagctcag ttggttagag caccaccttg acatggtggg ggtcggtggt
    11761 tcgagtccac tcggacgcac cagattttct taatctggtc ttctcctttt tccctctgtt
    11821 tcttctctgt atccaatacg ttaaaagatt tacactgtct tcgtatgcgt tatcagaagg
    11881 agaatcgcta tggcaacttt gttacaactt cattttgctt ttaatggccc atttggcgac
    11941 gcaatggctg agcagcttaa accgcttgct gagtcgatta atcaggaacc tggttttctg
    12001 tgga
//
LOCUS       DAJTYD010000119        11882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTYD010000119 DAJTYD010000000
VERSION     DAJTYD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.119"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(38..352)
                     /locus_tag="OO585_002875"
                     /pseudo
     CDS             complement(38..352)
                     /locus_tag="OO585_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(467..865)
                     /locus_tag="OO585_002876"
     CDS             complement(467..865)
                     /locus_tag="OO585_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCR6630401.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1105..3519)
                     /gene="gyrB"
                     /locus_tag="OO585_002877"
     CDS             complement(1105..3519)
                     /gene="gyrB"
                     /locus_tag="OO585_002877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6630402.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3548..4621)
                     /gene="recF"
                     /locus_tag="OO585_002878"
     CDS             complement(3548..4621)
                     /gene="recF"
                     /locus_tag="OO585_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6630403.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4621..5721)
                     /gene="dnaN"
                     /locus_tag="OO585_002879"
     CDS             complement(4621..5721)
                     /gene="dnaN"
                     /locus_tag="OO585_002879"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6630404.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5726..7129)
                     /gene="dnaA"
                     /locus_tag="OO585_002880"
     CDS             complement(5726..7129)
                     /gene="dnaA"
                     /locus_tag="OO585_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6630405.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7426..7506
                     /locus_tag="OO585_002881"
     CDS             7426..7506
                     /locus_tag="OO585_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630406.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7736..7876
                     /gene="rpmH"
                     /locus_tag="OO585_002882"
     CDS             7736..7876
                     /gene="rpmH"
                     /locus_tag="OO585_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6630407.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7893..8252
                     /gene="rnpA"
                     /locus_tag="OO585_002883"
     CDS             7893..8252
                     /gene="rnpA"
                     /locus_tag="OO585_002883"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6630408.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8216..8473
                     /gene="yidD"
                     /locus_tag="OO585_002884"
     CDS             8216..8473
                     /gene="yidD"
                     /locus_tag="OO585_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6630409.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8476..10122
                     /gene="yidC"
                     /locus_tag="OO585_002885"
     CDS             8476..10122
                     /gene="yidC"
                     /locus_tag="OO585_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6630410.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10228..11592
                     /gene="mnmE"
                     /locus_tag="OO585_002886"
     CDS             10228..11592
                     /gene="mnmE"
                     /locus_tag="OO585_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6630411.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11835..>11882
                     /gene="tnaC"
                     /locus_tag="OO585_002887"
     CDS             11835..>11882
                     /gene="tnaC"
                     /locus_tag="OO585_002887"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6630412.1"
                     /translation="MNILHICVTSKWFNID"
BASE COUNT         2718 a         3253 c         3067 g         2844 t
ORIGIN      
        1 gacaggggta tcgcttagtt tttcaatttc ctatacgtga accgacttcg cgagagagtt
       61 tttccaggaa acgatagtcg tcataactga gagcagtttg cgccacggtg ctaccatcag
      121 cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc tgttccatat
      181 gcagctcttt caggtagttg tgaacgcctg cataagggcg acccgtcgtt agcacgacat
      241 tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa atggtgtgat
      301 cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc atgagttccc
      361 caggtagatt agttcctgac ccatgctaac gcgattccgc tcaaaaatca gtacaacacc
      421 cgagggaaaa gggggatgca acgcgggttg catgctcccc atttgcttag cggaagagtt
      481 tccctttcag cagttccata cctgcggaaa gcagatcgtt attggcttgt gaagacactt
      541 caccttgcgg tgagagcgca tcaataatct tcggcaattg ttctgccagt aaactggaag
      601 ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt gtgccgggcg
      661 ccgattccag ttgctcgccg ctaactgatt gattgctctg ctgattactc aaccaggttg
      721 agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga atgccgccct
      781 gctcctcaac ccaacttaaa atagcctgat atttccccgc atcgcctttc agaaaggcac
      841 cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt gttgacccaa
      901 aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg cggagatgat
      961 ctccgccctt tttttatagc ttcttgccgg atgcggcgtt aacgtcttac ctggcctaca
     1021 aaatcattaa aattcaatac attgcaagat tttcgtaggc ctgataagcg tagcgcatca
     1081 ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca gggcgttctc
     1141 ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga acaacaggtc
     1201 agcggcaatc gcatccttaa cggtaacgcg cagcatacga cgactttccg ggtccatggt
     1261 ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct ggatggagag
     1321 accgcgacga gactctttca ccagccagtc cagcgcctgc tcgaagctgg ctaccggctg
     1381 acgacgctcg ccacgttcga taaatgcatc ttcttccagc aagccacgca gtttctcacc
     1441 cagcgtgcag atacgacgat attcgccacc agtgataaat tcgtgatcca gcggatagtc
     1501 agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt gttcggcatt
     1561 ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca gttcgctgac
     1621 cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcggctt ccgtcagcgt
     1681 cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca tgcggttgat
     1741 cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc cagccaatgc
     1801 cggtgcgctg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga tctggtactg
     1861 atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca ctttgtacag
     1921 cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct gacgatagaa
     1981 gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg tcatgatgat
     2041 gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac cacagccaag
     2101 cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc gcgctttctc
     2161 aacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt tacgcccctg
     2221 cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa gcgccggatc
     2281 gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg cacctttacg
     2341 gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat cgataatttt
     2401 gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca gcagttcgtt
     2461 catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt tggtctggga
     2521 ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt cacgcgcatc
     2581 gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca tgtaggcgtt
     2641 cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt cacgctgcgg
     2701 aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact gcaacgccac
     2761 ttcgacgcca ataccgtctt tttcggtgga gaagtagaag atattcggat ggatcggcgt
     2821 tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt ggaagtggtc
     2881 ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga ggaacgacaa
     2941 ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg tgaaggtttc
     3001 gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg taaccgccag
     3061 cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct cgcgctggat
     3121 aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc cgtgcagacc
     3181 gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa cggtcatgat
     3241 cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc cgcgcccgtc
     3301 atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt tacagtgacc
     3361 cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt gcagaccggt
     3421 gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat ccagcccttt
     3481 caggactttg atactggagg agtcataaga attcgacatc aacgtttctc gctcatttat
     3541 acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa ttttcgtccg
     3601 acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat tgcgtcgctt
     3661 ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca aaatcatcta
     3721 tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc gccagacgta
     3781 aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc ggcgcaccgt
     3841 cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt agctggcgat
     3901 cgcgttcaaa attacgttcc agcacctcag catattctgt ctctttctcc cagccgcgct
     3961 ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaagtatcg gccatatcag
     4021 ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccacc agcgggatca
     4081 gctctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc gccgcattgc
     4141 gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt tcgttgtgaa
     4201 agcatgccca gtcgagaaat gctcttctgt atttggggcc gccgttgagt aaagtaaacc
     4261 cttctggcgt tatcagctgc atcggcatca ggtgcgccag ctccgcgact ttatgcccgt
     4321 cagtaccgtc gatgcggact ttgctgtcgc cttgtttgtc tttggttaag ccaatcgccg
     4381 tctcgcgctc ttcgccctgt aatcgcccat ggagaacaaa tgcctcctgc tcatggcgaa
     4441 tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta tagatggctt
     4501 ccagcacact ggttttgcca ctgccgttgg cacctaccag aaagttaaag ccgggagata
     4561 aggcgagatc cgcggtttcg atattgcgga aatcgcggat caacaagcgg gtgagggaca
     4621 ttacagtctc attggcatga caacataagc cgcgctctgg ctggccgcat cttcaatctg
     4681 cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca gcgcgttcag
     4741 aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc tataggtaac
     4801 gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtgattt tcagctggtt
     4861 ttcgctgaca taaagacgta cgccgcggaa tttctcgtta gagagaattg ccgcacgggc
     4921 aaacgcctgc ttgagcagat cgcagccagc ttccagatgt ttgtccgggt tcttcggtag
     4981 aacgcggcgg taatccggga agcgaccatc caccagtttg gaggtgaaga taaagtcgcc
     5041 aacgtgggca cgaatgttat tgctgccaat ctgcacgcgc agcggattgt cgccgccgtc
     5101 gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat ggcttggcaa
     5161 agattgacca attggcattg aacagactgc cagacggtga ccatcggtcg ctacggtgcg
     5221 cagttcttcg ccttcggttt caaacagcat accgtttaag taatagcgaa catcctgatg
     5281 cgccatcgaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg gcagggtaaa
     5341 ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca gggtagacag
     5401 cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct gcacggcaat
     5461 ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg gaacggtcgt
     5521 tgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc accatttcca tctcgagatc
     5581 agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac cgagaatcgg
     5641 tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta ataaatgctc
     5701 acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa tttgaaaaat
     5761 cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag gcatgaagca
     5821 ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc cggcagactg tggttagtca
     5881 gctctttcgc cagcgccatc gccatctggc gcggacgcgc caccgagcgg gatcgacgct
     5941 tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc tgaatattgt
     6001 cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca cgcacgaagt
     6061 cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc agcgcccctt
     6121 ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact tcgcccggca
     6181 aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacacgggtt tccagctctg
     6241 gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga tcctcaacgc
     6301 cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta ccttccagca
     6361 gggcgttgaa ggtatggaaa aactcttcct gagatcgttc tttattagca aaaaactgaa
     6421 tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct tcgatcgcgt
     6481 tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata taaaccactt
     6541 tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagtagg tgagttttac
     6601 ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg ttatccgcca
     6661 cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag ttatcaaacg
     6721 tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg acgttatccc
     6781 aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc tgtgcagggg
     6841 ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg gtgccgactt
     6901 caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca ttgatattat
     6961 taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac agggccagcg
     7021 tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct gtggctggta
     7081 actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg gcggactcca
     7141 ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga cgtacgtcga
     7201 aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc ccgatcaaga
     7261 tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga tcttctgttt
     7321 ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt gtaaacgatc
     7381 ctgccgcgag gcgggcacga tttacgccac atattgaaaa atttaatgac catagacaaa
     7441 aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac ccatcagccc
     7501 gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc gtacattttc
     7561 cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact ccggagtgta
     7621 caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc gtttcatttt
     7681 tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa tcgccatgaa
     7741 acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc gtgctcgtat
     7801 ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc gcgctcgtct
     7861 gaccgtttct aagtaatcaa gctaacccct gagtggttaa gctcgcattt cccagggagt
     7921 tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa cgggctggca
     7981 cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt atcggtctta
     8041 cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa cgtctgacgc
     8101 gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg gtggtggcga
     8161 aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa aaattatggc
     8221 gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct atcaacgcct
     8281 gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa gctacggaat
     8341 tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga aacgcgtatt
     8401 aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac catttgatac
     8461 cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt gctgttcgtg
     8521 tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca ggcccaacag
     8581 accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt accggccagt
     8641 ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat caacacccgt
     8701 ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa ctctacccag
     8761 ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag cggtctgacc
     8821 ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa cgttgaaaaa
     8881 gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac gtataccgac
     8941 gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta cgctgtcaac
     9001 gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctccac ctttggtcag
     9061 ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa cttcgcactg
     9121 cacaccttcc gcggcgcggc gtactccacg cctgacgaga agtacgagaa atacaagttc
     9181 gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg ggtggcaatg
     9241 ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa caacttctat
     9301 accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc ggtactggtt
     9361 cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga aatccaggac
     9421 aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg gttgtggttc
     9481 atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg taactggggc
     9541 ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct gaccaaaacg
     9601 cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc aatgcgtgag
     9661 cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta caaagctgag
     9721 aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat cttcctggcg
     9781 ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc actgtggatc
     9841 cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg cgtaacgatg
     9901 ttcttcattc agaagatgtc gccgaccaca gtgaccgacc cgatgcagca gaagatcatg
     9961 acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg tctggtgctg
    10021 tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta ccgtggtctg
    10081 gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga gttttcgcta
    10141 aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa gcaccgcgca
    10201 tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt agcccaggcc
    10261 acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa agcccgtgaa
    10321 gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta tcttccgttt
    10381 aaagacgccg acggcagcgt actcgatcag gggattgcgc tatggttccc tggcccgaac
    10441 tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt gatcctcgac
    10501 ctgctgttaa aacgtattct gaccattccc ggcctgcgga ttgctcgccc tggtgagttt
    10561 tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc gattgccgat
    10621 cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct gcaaggcgca
    10681 ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat ttacgtcgaa
    10741 gcggcaattg atttccccga tgaagagatc gatttcctct ccgacggaaa aattgaagcc
    10801 cagctcaatg acgttattgc cgatcttgat gcagtgcgtg ctgaagcacg tcagggtagt
    10861 ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc ctaacgccgg taaatcgagc
    10921 ctgttaaacg cgctggcggg gcgtgaagcg gcaatcgtaa ccgatatcgc cggaactacg
    10981 cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat catcgatacc
    11041 gccgggctac gtgaagccag tgacgaagta gaacgtattg gtatcgagcg cgcgtggcag
    11101 gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacaac agacgccgtg
    11161 gatccggcag agatctggcc ggaatttatt gcccgtctgc cagcgaaact gccgatcacc
    11221 gtggtgcgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga agtgaacggt
    11281 cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct gcgtaaccat
    11341 ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc gcgtcgtcgc
    11401 cacctacagg cgctggaaca ggcagcggaa catctacaac agggcaaagc gcaactgttg
    11461 ggagcctggg caggtgaact gctggcggaa gagttgcgtc tggcacagca gaacttaagc
    11521 gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc cagcttctgt
    11581 attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg cttcgcttca
    11641 ttgttagcac tcctgttatt cctcaaccct ttttttaaac attaaaattc ttacgtaatt
    11701 tataatctta aaaaaagcat ttaatattgc tccccgaacg attgtgattc gattcacatt
    11761 taaacaattt tagaatagac aaaattcttg agtattatac tgtagcctcg tgtctttcga
    11821 ggaataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg ttcaatatcg
    11881 ac
//
LOCUS       DAJTYD010000120        11473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTYD010000120 DAJTYD010000000
VERSION     DAJTYD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.120"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..330)
                     /gene="osmE"
                     /locus_tag="OO585_002888"
     CDS             complement(<1..330)
                     /gene="osmE"
                     /locus_tag="OO585_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6630413.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQA"
     gene            532..1359
                     /gene="nadE"
                     /locus_tag="OO585_002889"
     CDS             532..1359
                     /gene="nadE"
                     /locus_tag="OO585_002889"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6630414.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            1589..2476
                     /gene="cho"
                     /locus_tag="OO585_002890"
     CDS             1589..2476
                     /gene="cho"
                     /locus_tag="OO585_002890"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6630415.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(2436..3011)
                     /gene="ves"
                     /locus_tag="OO585_002891"
     CDS             complement(2436..3011)
                     /gene="ves"
                     /locus_tag="OO585_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6630416.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3214..3684)
                     /gene="spy"
                     /locus_tag="OO585_002892"
     CDS             complement(3214..3684)
                     /gene="spy"
                     /locus_tag="OO585_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6630417.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4014..4982)
                     /gene="astE"
                     /locus_tag="OO585_002893"
     CDS             complement(4014..4982)
                     /gene="astE"
                     /locus_tag="OO585_002893"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6630418.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(4975..6318)
                     /gene="astB"
                     /locus_tag="OO585_002894"
     CDS             complement(4975..6318)
                     /gene="astB"
                     /locus_tag="OO585_002894"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6630419.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6315..7793)
                     /gene="astD"
                     /locus_tag="OO585_002895"
     CDS             complement(6315..7793)
                     /gene="astD"
                     /locus_tag="OO585_002895"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6630420.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(7790..8824)
                     /gene="astA"
                     /locus_tag="OO585_002896"
     CDS             complement(7790..8824)
                     /gene="astA"
                     /locus_tag="OO585_002896"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6630421.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(8821..10041)
                     /gene="astC"
                     /locus_tag="OO585_002897"
     CDS             complement(8821..10041)
                     /gene="astC"
                     /locus_tag="OO585_002897"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6630422.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10487..11293
                     /gene="xthA"
                     /locus_tag="OO585_002898"
     CDS             10487..11293
                     /gene="xthA"
                     /locus_tag="OO585_002898"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6630423.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2789 a         3119 c         2867 g         2698 t
ORIGIN      
        1 agcctgtgga tcagtgtcgt attcagcaca ggtctgataa ccggagttga tgacatgtcc
       61 ggtatcatct aacgcgacaa agtaggtttc tgctttacca tcacgttgac ccaggatgta
      121 ggtctggcaa gtaccacgag catggatcat gctcacttca gacgaaggtt tacccgcaat
      181 ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct ttcaccacag gctgtacaaa
      241 ctggtctttg gtacgatcat aagccgtaca acccgccagc atggttaata ccgccgctgc
      301 actcagaatt cctgccatat tcttgttcat attccgtcct cttgtttatc agcgtgttag
      361 ataagcctgg aatacattgg gcgctttttc aagcccgtga acgaaacggc tccgctttca
      421 gaggattcct gtatgacgtt ttatccacca ttcaacccgc tgtcgcttgt cgtttcagta
      481 gcgacgggtt agctttaagg aagttttgtc ttttctgtct ggaggggttc aatgacattg
      541 caacaacaaa taataaaggc gctgggcgca aaaccgcaga ttaatgctga agaggaaatt
      601 cgtcgtagtg tcgattttct gaaaagctac ctgcaaactt atccgttcat taaatcactg
      661 gtgctcggga tcagcggcgg tcaggactcc acgcttgccg gtaagctgtg ccagatggcg
      721 attaatgagc tgcgccagga gaccggcaac gaatcactgc aatttattgc cgtacgcctg
      781 ccctatggtg ttcaggccga cgaacaagat tgccaggatg ccattgcctt tattcaaccg
      841 gatcgcgtat taaccgttaa tatcaagggc gcggtattgg ccagcgaaca ggcgctgcgc
      901 gaagcgggca ttgaactgag cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg
      961 aaagcacaat atagcattgc gggtatgacc agcggtgtcg tggtgggcac cgatcatgca
     1021 gcagaagcta ttaccggatt cttcactaaa tatggtgacg gcggtacgga cattaatccg
     1081 ctgtatcgtc tcaacaaacg tcagggtaaa cagttactgg cggcattagc ttgcccggaa
     1141 cacctttata agaaagcgcc aacggccgat ctggaagatg atcgcccttc tctgccagat
     1201 gaagtggcac tcggcgtgac ctatgacaat atcgacgact atctggaagg gaaaaacgta
     1261 cctcaacagg tcgccagaac aatagagaac tggtatctga aaaccgaaca taaacgccgt
     1321 ccgccaatta ccgttttcga tgatttctgg aaaaagtaat aatttgcaca tattggattg
     1381 tgcgaaaaag agtaatttgt tcacgccgga tgcggcgtga acgccttatt cgacctataa
     1441 aactatgcaa attcaataca ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag
     1501 acggtgtaac ctttgtcatc gacccgcctc ttttttaatc gcttcccgcc tgttacactg
     1561 gatagataac cagcattcgg agtcaacagt ggtacggcgt ttaacttctc cgcggctcga
     1621 atttgaagct gcggcaattt atgaatatcc cgaacattta cgttcattcc ttaatgactt
     1681 acccacccga ccaggggtgt atctgtttca tggtgaaagt gacaccatgc cgctctatat
     1741 cggcaaaagc gttaacatcc gcagccgcgt cctttctcat ttacgtaccc cggatgaagc
     1801 cgccatgcta cggcaatccc gacggatcag ctggatatgt accgccggtg aaatcggcgc
     1861 tctgctcctt gaagcgcgat taatcaaaga acaacagccg ctgtttaata aacggttgcg
     1921 ccgcaatcgt cagctctgtg ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc
     1981 caaagaggtg gatttttcac gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc
     2041 agctttgcaa gcattgcaga ccatcgctga tgaacaaaaa ctttgttatg gcctgctggg
     2101 actggaaccg ttaagtcgcg gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg
     2161 agcatgctgc ggtaaagaga gccatgagga acatgcgcta cgcttgcgcc agtctctgga
     2221 gcgtttgcgg gtggtgtgtt ggccttggca aggggcggtg gcgctgaaag aacagcaccc
     2281 ggaaatgact caatatcata ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct
     2341 ggaagacgcg acaacgttaa ttcggacacc cgccgggttt gatcacgacg gttataaaat
     2401 tctttgtaag ccgctgcttt ccggtaacta tgaaattact gaacttgatc cggcgaatga
     2461 ccagcgagcc agttgatttc actgaacaac agtttacctt gcggttgtaa aagccgcagg
     2521 gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag tgagcaattt atcgcccagt
     2581 tgccacgccc cgttaatcac aaacaccacc ccgccgcgtg agccaaaggt agtaaacgta
     2641 cgttcggcaa ttctgacttt cgccttacag acatccaggc gtgtcatgat gttgaaatcc
     2701 atcgacatct gcccttccgt cagtttcgct tttaccacct ggtccgctgc aaaggcaaaa
     2761 ggctgtaacg gttttaaggt atggttaaag cggtctgcgc tttcaaggag catctcgccg
     2821 ccttccagca acgtcactat cctttccatg ccgggaaaca aagaaaactc cccattcgcc
     2881 gcgatggatg caatgctggc acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa
     2941 atttcgcgcg tttcaccggc agcatttcgc cacagattca ccgacatttt acgcatatca
     3001 aagtattcca tactcgctcc cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg
     3061 atggttatcg gcaactcggg tgatttacgt tagtggtgat caggcttttt ttacaactgt
     3121 ttgagaagag aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca
     3181 agaccggcgg tcttaagttt tttggctgaa agattattca gcagttgcag gcattttacc
     3241 ttttgccgct ggacgttctg tcagacgctt ctcaaaatta gcattaaatt gctttttctg
     3301 ttccggcgtc aggatgttgt aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc
     3361 tttgcgctgt tcttccattt ttgcgatctg cgcttcagct tttgctttat cgaaggtatc
     3421 gctgacaatg atgtcatgca ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc
     3481 acgctggcct ttcatgattt cgcggatctg ctgtttctgc gcgtcggtca ggttcaggtc
     3541 tttgaacatc atgtcctgat gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc
     3601 gtcagccggt gctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
     3661 aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct
     3721 ttcttaagta gcgtgccgtg ttgacaagat taactttact gactttagcg tcaattaatc
     3781 agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa
     3841 aagaaaattt gatggagagt gatgagagaa tattacaaca caatgatttt gcagagatta
     3901 tgaagaacta tactggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat
     3961 taatttataa gaaagcaact taatacccgc agaatgattt ctgcgggtaa gtattagctt
     4021 attttttcga gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt agggaataac
     4081 acatactcta catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt
     4141 cctttctcaa acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc
     4201 gagtgacgag taatttgcga aaccacccga taacggagcg gcggtgttct cacgataccg
     4261 acactctcac cagatagcag cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga
     4321 tcgttttgcc caaagggcaa cgctttgcca agttccagcg tacaggccag cgcgccaaaa
     4381 tgtctggcgc tgaaatgggt aaacgtacca ccaggttcct gatggaacac cagcgcctcc
     4441 agccccgccg cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt
     4501 aatacaccga actgcggatg caaggagcca cgaattgcgg tatgtagatc aaggtgccag
     4561 cgcacagatt ctttgccctg gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc
     4621 cgacaggttt ctccgctttc agcaaatagc tgccaacgac cgccaaacat tcgattcata
     4681 tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagc
     4741 aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc gtccagcatt
     4801 tccacaggtg ccgtctcatt accgtggatc cccgcagaaa tcaccagcgc gccttgcggt
     4861 ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc agcgccagcg aacgccgttg
     4921 atttctcgct cggtgataac cggtttttta cccgttaagg tcagagcaag aaaattatcc
     4981 attgccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa
     5041 tacatccagt gcttcccgcc cttcgcgcag caattgcggg tcggccagat cggcagcagt
     5101 aagacgatcg cggtagtaac gatccaccca gtcattgagc gcattaaaca aggtatcgtt
     5161 catcatcacc gccggattca ccgcccggcg ttcttcttct gtcaatacca cccgcaaccg
     5221 caggcacgcc gggccgccgc cattcgccat gctttcacgt aagtcaaaga cttttagttc
     5281 gctaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgttc
     5341 ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct
     5401 gttaaacaga taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat
     5461 cgccataaaa ccgttcaccc gcgcccgcag gtttgccagt aactgtgact ggcgagcgaa
     5521 cgcctgttgg tggcaaaaca gcacctggcg gttacttacg gcaatcacgt cattatgaaa
     5581 aacaccctgg tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt ggggattcac
     5641 ctgattaagc cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga
     5701 aggccgggta tcattgcctt cttctcgccc gtagacaaaa agttgcatac ccggctcacc
     5761 gtaatgaccg ccgagacgat tgtggtttgc cgccccctca tcaccgagca acgctacctg
     5821 cggcaacgcc gaatggacgc taaatttctc ttcgtcgtta aaaatagctt ttaacagcga
     5881 ttcagtgacg ggcgcttcca gcgaacggtg aaatttattg ttcaggttgg caacggtgag
     5941 atgcactttg ccatccagcg tatcggcaga tggcgcgatc gttgccgcat tggctaccca
     6001 cattggcgaa gcggagctga cgctggaaag ccagtgcggt gcctggcgtg caactttttc
     6061 cagtacctgc tcatcgctac cactgaatcc caactgacgc agcaccggaa taaacggacg
     6121 ctcgtgcggc gggatcacgg cctgggggaa tcctgcatcg gcaagggttt tcattttcag
     6181 taagccctgt ttcgccgcca gtcgcgggtt agacacctga aaacggtgac gggtagaggc
     6241 ttcattacca aacgacaggc cagcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt
     6301 aacttcccag gcgttcatcg caccacctca tcggaaaaat ccagcccggg gttaagcgtt
     6361 gcgggcaaag ttaacgagtc cgactccagg ctcgccatag gccatgcgca gtaatctgcg
     6421 gcataccagg cgctggggcg atggttaccg gatgcaccaa tgccgccgaa tggcgcggta
     6481 ctggcagcac cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac
     6541 agttgatcga atttttcccg ttcgggggaa accagaccgc aagagagtcc gaagcgagtg
     6601 ttattcgcca ttagaatcgc ttcctcgaaa gaatcataac gccagacgcg caataacggt
     6661 ccgaacacct cttcatctgg tacgccagca acgcctgtca tttcaatgat ccccggcgtc
     6721 agcaacgatg tctctaattg taataagcgc ggcgcaagca gggttcgtcc gcccatcgct
     6781 tccagttgct gccaggcagt aaccacctgc tgtgcggcct gttcagaaat cagcccgcca
     6841 ataaacggct gcggttcgtc atcccagttg cccggcgtta atcgctggct gacggcaacc
     6901 agacgagcaa gaaacgcatc gccctgcgct ccgcttttga gcaataaacg gcgggcgcag
     6961 gtgcagcgtt gcccggctgt gacaaacgcc gactgaatgg tcagatggac agccgcgtcg
     7021 atatccgcca cctcatcgat aattagcgga ttattaccgc ccatctcaag ggcgagaatt
     7081 ttctccggct gaccggagag ctggcgatgc agctggtagc cggtattggc gcgaccggta
     7141 aacagcaaac cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc acgcccgccc
     7201 tgcaccaggt tcagcacgcc cggcggcaag ccagcctgct gccataaacg cattaccgct
     7261 tcgccactcc acggtgtcag ttcgctgggt ttaaagataa tggtgttacc tgccagcaat
     7321 gccggaacga tatgtccgtt cggcaaatga ccagggaaat tatacggccc aaacaccgcc
     7381 agcacgccgt gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc actacgctgc
     7441 tcgccggtac gaacgtgata cgccttaatt gatatcgcga ttttattgat catcgccgtc
     7501 acttcggttg ccgcttccca gcgcagctta cccgtttctc tggcaataat cgcggttaat
     7561 tcggctttat tgctttccag caatccggca aagcgttcga caacgacctg acgttcagcc
     7621 aatgagagcc gcgcccagcg cggaaacgcc gcacgggctg cccgacaagc ctgttcgacc
     7681 tgagcggcat cggcatcatt gccttgccat aacacctcgc ccgataccgg attacgcttc
     7741 acacgcgatg cgccctggcc cgttatccag tcaccgttaa tccataaagt catgctgttt
     7801 tctccactgc gcacaggcgc accagacgaa cgcgatcccc tgcgtggcat ttgagggcat
     7861 ccagttgtgc ggcggttaaa atcaaacgct cggttgccgg atcggtacgc gccagcacca
     7921 cgcggaaatg gtgataattt tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag
     7981 gctgcccttc tgccacttcc accagccgac ttttacggat ggcgcgcacg cggtcgatgt
     8041 cacactcaag cgttggcccg ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt
     8101 ctttctccag caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct
     8161 gggcttcctg ggataaaaag tgggtataga tcggatgttt cggcatcagt tctgcaataa
     8221 atgccttttg cccggtgccg cagagaaaat cggcgcggct aaaatccatc gaaaagaagc
     8281 gtttaccgag gctttgccag aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt
     8341 cagcaaccac tttgtcatta aacttgtcgc gaaaagccgc cataaacata aagcgcgatt
     8401 tcgacagcaa atagccgttg ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca
     8461 gctcgctgct gccggtgtga tcgttactaa gaaacagcgt cggcaatgcg ttatagacat
     8521 tcagctcttt tgaggcgtga accaacgtgc cgacccgata gttgtgccag ggatcgttca
     8581 gcccaaccgc cacctcaatg gcgcaaatcc ccgccacggt gcctgtctcg ctatcttcca
     8641 gcacgaacac atagccctgc tcgcttttgg gcagttcgcc ttgccaggtt ttgattgccc
     8701 tttcgatacg cgcggaaagc gtggcttcat tagcgggaag cgacgtcagg ccgccgcccg
     8761 ttttgctggc aagctgcatc agcgccgaga catctgagcg ctcaacggga cggatgacca
     8821 tcatgatgaa cctcggctaa caaaatgttc gcaagcagct gcaaagcgat ccagtccggt
     8881 cgtcacctct tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc
     8941 aatcagtacc ataacgcctg ctttcgccgc ctcctgagag atctgtttcg cttgtccggc
     9001 gtaatcggca ttcagtacac agccaatcaa caaacctaag ccgcgaactt cactgaacaa
     9061 accgtagcgg tgattaatgg tattaagacg ctcaacaaac cagtcgtgac gctgtttaac
     9121 gccattaagc atctctggtg tgttgatgag ctccagcact ttgcccgcca ccgccgaggc
     9181 cagcggattg ccgccatagg tggtgccatg agtgccaacg gtcatcacgc tggcgcactc
     9241 ttcggttgtt aacaacgcac cgaccgggaa atcgccgccc agcgctttgg cggtagttaa
     9301 cagatcaggt gtcacgccgt agtgcatata ggcatacagt tccccggtgc gcccgacgcc
     9361 ggtttgtact tcatcaaaaa tcaacagcgc attgtggcgg tcacacaatt cacgcagacc
     9421 ttgtaaaaac gcgttgctgg ctggcaccac accgccttcc ccctggatgg gttcgacaat
     9481 caccgcacag gtggcgtcgt caatcagcgc gctggcagag ttaatatcgt tatatgcagc
     9541 atgacggata tctggtggca gtggcgcaaa atcctgtgaa taggctggct gcccacccgc
     9601 actgacagta aacagcgtgc gaccatgaaa cgcattttta aacgccatga taccgctctt
     9661 atggctgccg tagcggtcgt gagcgaattt acgcgccagt tttagcgccg cttcgttggc
     9721 ttccgcaccg gagttacaaa agaagacgcg atcggcaaac gtggcgtcga tcaatttttt
     9781 cgccagtcgc agtaccggct cgttggtgta accgttgccg gtatgccaga acttactcgc
     9841 ctgttcgttc agcgcttcac gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat
     9901 gccacccgcg aagtcgatat actctttccc ctgctgatcc cacaagcgcg aaccttcgcc
     9961 acgtaccggt ataaagggtg ccggagcgta aacaggtatc atccattcat caaagttttc
    10021 acgcgtaatt ggctgagaca tagcgacctc tacagtaaat aattcgttat ttatatgtta
    10081 acaataagta atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag ccagtgataa
    10141 aagtgcataa acggcggagg ctaactggaa atcaaggagt tataaccaaa ccatatgcat
    10201 ttaaagtgca tataaagtga atacgtttgc gatgtgggtg aataaaaaga ataaaaaacg
    10261 caatgttatg cagaagtaaa atataattct ggaattgtga tcattgacga aatttactgg
    10321 aaattactgc gccattctga cgcagcgcgc accaaaagcg ggcatttttt gcgccatcgt
    10381 tgacatcatt aacaaccatc gatcaaatca cttaacaaca ggcggtaagc aacgcgaaat
    10441 tctgctacca tccacgcact ctttatctga ataaatggca gcgactatga aatttgtctc
    10501 ttttaatatc aacggcctgc gcgccagacc tcaccagctt gaagccatcg tcgaaaagca
    10561 ccaaccggat gtgattggcc tgcaggagac aaaagttcat gacgatatgt ttccgctcga
    10621 agaggtggcg aagctcggct acaacgtgtt ttatcacggg cagaaaggcc attatggcgt
    10681 ggcgctgctg accaaagaga cgccgattgc cgtgcgtcgc ggctttcccg gtgacgacga
    10741 agaggcgcag cggcggatta ttatggcgga aatcccctca ctgctgggta atgtcaccgt
    10801 gatcaacggt tacttcccgc agggtgaaag ccgcgaccat ccgataaaat tcccggcaaa
    10861 agcgcagttt tatcagaatc tgcaaaacta cctggaaacc gaactcaaac gtgataatcc
    10921 ggtactgatt atgggcgata tgaatatcag ccctacagat ctggatatcg gcattggcga
    10981 agaaaaccgt aagcgctggc tgcgtaccgg taaatgctct ttcctgccgg aagagcgcga
    11041 atggatggac aggctgatga gctgggggtt ggtcgatacc ttccgccatg ctaatccgca
    11101 aacagcagat cgtttctcat ggtttgatta ccgctcaaaa ggttttgacg ataaccgtgg
    11161 tctgcgcatc gacctgctgc tcgccagcca accgctggca gaatgttgcg tagaaaccgg
    11221 catcgactat gaaattcgca gcatggaaaa accgtccgat cacgcccccg tctgggcgac
    11281 cttccgccgc taatttagca gctctcctgg ctcaaactgg gtcaggagaa ttaaccttga
    11341 gaaaaatcaa caaactgtca gtaatgattt gttgcctgcc gtcctttgtt ataccgtcac
    11401 tgcgttttta gttgtctgac cacttctcta ttatcaagtt tgatatagga aactccacga
    11461 tgaatgctga gcg
//
LOCUS       DAJTYD010000121        11447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTYD010000121 DAJTYD010000000
VERSION     DAJTYD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.121"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            15..188
                     /locus_tag="OO585_002899"
     CDS             15..188
                     /locus_tag="OO585_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6630424.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(237..1309)
                     /locus_tag="OO585_002900"
                     /pseudo
     CDS             complement(237..1309)
                     /locus_tag="OO585_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1381..4125)
                     /gene="torS"
                     /locus_tag="OO585_002901"
     CDS             complement(1381..4125)
                     /gene="torS"
                     /locus_tag="OO585_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6630425.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4208..5197
                     /gene="torT"
                     /locus_tag="OO585_002902"
     CDS             4208..5197
                     /gene="torT"
                     /locus_tag="OO585_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6630426.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5170..5862)
                     /gene="torR"
                     /locus_tag="OO585_002903"
     CDS             complement(5170..5862)
                     /gene="torR"
                     /locus_tag="OO585_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6630427.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQT
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            5982..7154
                     /gene="torC"
                     /locus_tag="OO585_002904"
     CDS             5982..7154
                     /gene="torC"
                     /locus_tag="OO585_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6630428.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7154..9726
                     /gene="torA"
                     /locus_tag="OO585_002905"
                     /pseudo
     CDS             7154..9726
                     /gene="torA"
                     /locus_tag="OO585_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9723..10322
                     /gene="torD"
                     /locus_tag="OO585_002906"
     CDS             9723..10322
                     /gene="torD"
                     /locus_tag="OO585_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6630429.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10415..10720)
                     /gene="cbpM"
                     /locus_tag="OO585_002907"
     CDS             complement(10415..10720)
                     /gene="cbpM"
                     /locus_tag="OO585_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6630430.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10720..>11447)
                     /gene="cbpA"
                     /locus_tag="OO585_002908"
     CDS             complement(10720..>11447)
                     /gene="cbpA"
                     /locus_tag="OO585_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6630431.1"
                     /translation="DQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIFGQHARQS
                     RQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTLNVKIPAG
                     VGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVSPWEAALG
                     AKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMPPKPDENT
                     AALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2738 a         2969 c         3057 g         2683 t
ORIGIN      
        1 agaagaggtt aacagtgaat attgaagagt taaaaaaaca agccgaaacg gaaatcgccg
       61 actttatcgc gcaaaaaatc gccgagctga acaagaatac agggaaagaa gtctctgaaa
      121 ttcgcttcac cgcacgagaa aaaatgaccg ggcttgaaag ttatgatgtc aaaatcaaaa
      181 taatgtgatt ttgtgaacat caccccgtgc gaggtgatgt tccgcttgtt gctaatttag
      241 tgatcaatca ttggcgcttg tggaattaag cgtcggtaca attcctccgg caccgggctt
      301 tgccatactc ccgcatacat tgcgtaacca atcaccgcga acataatccc cagaaccagt
      361 agcgtcatta accagccaga caacgcaaaa gcttttttat ttgccgcagg tttttgcagt
      421 gaaaaggtca atgttgaggc taccggacat gactctacgc aagtcataca gccggtacat
      481 tccactgttc gtacctgaat taatttatct accgggatcc gtgatgggca attttttgcg
      541 catttgccac agccgataca actttcggca ttgcgacgaa tcttaaacgg cgataatagc
      601 gaaaccacgc ccatcagcgc gccatatggg caaagataac gacaccaggc atggcgaata
      661 aacaggctgg caatcagcaa aacggtcacg ctgattaatg tcgcggtccc catatgacga
      721 aagaaatcga gcatttttac gtccatcacc acgctgtagg gcgacaacat aaaatagtga
      781 atcgcctgag cgggcatcaa taacgcgata tagagaaaaa aactcaacag caaatacttc
      841 acgccgcgca gaggaatatc cagccagcgg ggaaggacac attgccgacc aaacagtttg
      901 ttaccgagat cgccgattaa ttcagaaagc gtaccaaccg ggcataacca tgagcaaaag
      961 gcctttttga gtaatagact gatgacgata aaagcgacca ataacagcat cgcggagcgt
     1021 ggacggacgg taactgacct gttgtaaggc tatatttcag attcatcaga ccggcaatcg
     1081 gtagccagcc ttcgatacct cccggtctgg cgacaaatgt cgtgctactt gccgtttcgt
     1141 aatagcgcac ccaataccag aacgtgatgg caatataaat attcattgcc aacagtaata
     1201 attgcgtcgc tttacgccag gtcgtggcat tgcgccagtc attccacggt aatttgccgc
     1261 ccgtcgtgcc tggccgccgc tgccagcggg ttcttttttt ctctgccatg attttgccag
     1321 tccgttaagt tgcataccaa atgccactat tttagttgtt cttaactggc tgatattgat
     1381 tcaaatcgcg ttcaggtctt tcttatgcaa ccatgcttcc agagcggcaa cactgcgtgt
     1441 aatttcttcg tgtggaaggg gggcagataa tggctgctgc tccagttgtg cgcatagctg
     1501 gctggcgata cgcattccca aactcgagca actgcttttt agctgatgcg cggcacgctt
     1561 tattttctcg ctatcctgac tggcgcgggc aatgtcgatt tcatcgagaa gcggcagggc
     1621 atgttgtgta aataatacca gccattcgtg gatcttctcc gtccccatta actgagcatc
     1681 ttcattgagt tgcgatacat ccagcgattg atcgttattg acttgcagtt ggagatagtg
     1741 cgccagtaac tgaccgagca cttcacgcgg caccggttta gggataatcc cgcggaataa
     1801 tgaactggta cgctggcgca gcgtttcgtc aatgacatgg gcgctaaagc caatcaaaac
     1861 cagcgacgga tattgctgtg ccagttgtcg ggcaagcgta atgccgtcga catccggcag
     1921 atcaaaatcc accagtgcgg cagcaaacgg ttcgctattt tgcagtgtct ctaaagcctg
     1981 cgcggcattg ccaacagcaa caacctgcgc accactggtg ttcagcatct caacggtaat
     2041 tcgctgggtt agcgggttat cttcaattaa cagcaaacgt aaaccgtcaa gacgcaccgc
     2101 ctgattgact gtttttggca cgggtgccgt ggcaacacgt aacggcaagc gtaaacaaaa
     2161 acagcttcca acctccggcg tgctggtggc gctcagttcg ccgcccatcg cctgggccag
     2221 acggctactg atagtcagtc ccagcccggt gccgccgcgt ttgccgctta cctgcacaaa
     2281 tggctggaag atttctgcca gtttcgcggg atcaataccg cagccgctgt cttccacttc
     2341 gaccagccat tgctcgccat cagtgcggct acgtaaaacg atctgccctt cgtcagtaaa
     2401 acgcagggcg ttgctcaaca ggttggttat aacctgacga atacgtcgcg gatcgcccat
     2461 taacgcggtc ggtacatcgt cggcaattgc tgttgccagg cgaatcgggc gacctttcac
     2521 ccgtccgctc attaattgca gggtactttc cagcagcggg cgcggttcaa agggctcatc
     2581 gctgaccgaa acattcttgc cacctgcttc gatagccgaa taatcgagaa tatcgttgag
     2641 gatggtcagc aacgattcgc cagagtcggt aatcgcccgc aaatcatcac gctgggcgtt
     2701 aagtgcgggg ttatctgcca gcagttgagc agtgccgaga ataccgtaca gcggtgtgcg
     2761 gatctcatgg ctcatcgccg ccagaaaggc cgattttgcc tggctggctt tttctgcttc
     2821 cgcccgtgcc tgtcggtgtt ctatcaccag ttcctgcaat tcagctgtac gcgctttgac
     2881 ctgcgccgcc agctgttcac ggtggcgatt cagtgcatga acagtgctgc gaaacgcatc
     2941 catcagccgc ccgatggtat ccagctcccg tacgccagcg gtttccggga aaggggagtc
     3001 aatatcaccg tccagcagcc gttgcagcgc ctgcgtttgt tcggcaagtg gacgcgtgac
     3061 tgagcgataa accacgcgcc aaaggatcag aatcagtgcg caaagtgaaa ccatccccag
     3121 caataacagg ctgtattgcc cgcgtgcact ggctttttcc agatgcgcca gtccgtgctg
     3181 attacgcagc tcaatggtgt cgaccagctg actgacttcg ctactaaact gcgcgaactg
     3241 ggcgatgtta ttttgtgcga gagtttgtag gtgattgctg atttcactgt cctgctgata
     3301 cagcgccagc aaatcgctat attggctaac ggtagttaac gttgttgcga cctgcgcacg
     3361 aacacccgga tcttcaatgc gtatttgccg acgttgcaga attttcaccg cattattgag
     3421 ctgcttttcc agcattggtg catttttctg gatctgctcc agccccagat tcatcaccat
     3481 ttgctgcacc cgcagagcgc taaggcgcag ttcattcatc tggttaacat actcaagatc
     3541 gatatcaatc agccgatcga gtgcactttc agcagcctga cgctgatctt gttcgatcaa
     3601 atcgtaaatc ccggcctggg tcgctccagc ggaagttgtc gcattattcg cctgaccttg
     3661 cgccaggcgt gcgatctcat cggcggcagc gactatctgc tgactgagtt gctgttgttg
     3721 ctggcgtagt tgcagacgcc gccccaccag ttccccttgc tgacgtaacg aacgggagat
     3781 ctcctgctcc tgttgttcaa tagcggtggt atcaaaacct tgttcccgta acgcttgcag
     3841 caacgcatta atcttcaggc tttgtgcggt gagcattcgc ccctgcgcct gccacatctt
     3901 ttcgttatcg gcactggtca ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct
     3961 caactggcgc gccatattca tggtaggaat caatgcctga gtgttgtctt tttccacctg
     4021 gctgataaag cgcaggttgt accatcccac cagggtactg gtcagggtta acagcgccat
     4081 cagggcaaag cccatccaga gtcttcgggt cagggttaaa ttcacggtcg gtgcacttta
     4141 ggtgaaaaag gttgagtcgc aaagcagaat gcatctagca taaagcctta ttattgatta
     4201 ggctatcatg cgcgtactgc tatttttact tctttccctt ttcatgttat cggcattttc
     4261 ggctgataac ctgttgcgct ggcatgatgc gcagcattac acggtgcaag cctctacgcc
     4321 gcttaaagcc aaacgcgcat ggaaactgtg cgcgctttat cccagcctga aagattcata
     4381 ctggttatcg ttgaactatg gtatgcagga ggcaggcggc tacagccagt tggctaccca
     4441 gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag gccattttgc tcggtagtag
     4501 cactacgtca tttcccgacc tgcaaaagca ggtagcaaat ctgccggtga tcgaactggt
     4561 aaatgctctt gatgatcccc tggtgaaaag ccgcgttggt gtaccctggt ttcagatggg
     4621 ctatcaacct gggcgatatt tggtgcaatg ggcgcacggt aaaccactga acgtgctgtt
     4681 gatgcccgga cccgataatg ccgggggcag taaagagatg gtcgagggtt ttcgcgcagc
     4741 cattgccgga agcccggtgc gtattgttga tattgcgctt ggtgataacg atattgaaat
     4801 ccagcgtaac ctgttgcagg agatgctgga acgccaccca gaaatcgacg tcgttgccgg
     4861 aacagccatt gcggcagagg cggcaatggg tgaagggcgt aacctgaaaa cgccgcttac
     4921 cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg ctgaagcggg gaagagtgat
     4981 tatggctgcc agcgatcaaa tgatctggca gggggaactg gcggttgagc aggccatcag
     5041 gcaattacag gggcaatcgg tgtctgataa tgtcagccca ccgattttag ttctgacgcc
     5101 gaaaaatgcc gaccgtgaac atattcgccg ctcgctgtca ccagggggat ttcgtccggt
     5161 ctatttttat cagcacacat cagcggctaa gaaataacat tcaccatgtt gcgtcaccag
     5221 taaatccgcg ctgagtttat gacgtaaacg acgaattaac acatcgacgg tgcgcaggtc
     5281 agggttttcc acccgacgcg cagaaagcat acgtagcaga cgttcacggc tgagaatttc
     5341 gcccggattc gtcacaaatg ccaccaacat ttcatactct gcgcgggtca gtttaatcgg
     5401 ctcgccatcc cgctccagcg tatggcgcga cacattcagg caataaccgg caaagcgata
     5461 gcagttgtcc tgagtgtgcg gttgagcttg tcgcgcgagg tcgattcgcc agagcagatt
     5521 tttcacccgt actaccagtt cgcgcagttc cagcggtttg gtgacgtaat cgtctgcgcc
     5581 catttccagc ccaacaatac ggtcaatccg atcgctgcgt ccggtaacca gaataatccc
     5641 caccgttgag cgttctcgca gggcgcgggt taacatcagg ccattttcat cgggtaagtt
     5701 gatatccagc agaattaaat ctaccggctg agtctgcata atttcccgca gcccggcacc
     5761 gctcgctgta acggaaacgg tatacccctc ctgagtgaag taagattgta atcgcgcctg
     5821 ggtaaccggc tcatcttcaa caataacaat gtgatgtggc atcagagggt tttactcatt
     5881 ctgttcatat tctgccgtaa gccgttcatc ctgaccagtg ccgctgttca tatttgctca
     5941 ttaagatcgc ttcactaaac cataatttta caggggctat tatgcggaaa ctctggaacg
     6001 cgctacgccg acccagtgct cgttggtcgg tactggcgct ggtcgcaatt gggattgtga
     6061 ttggcattgc gctgattgta ttgccacacg ttgggatcaa agtcaccagc acaaccgaat
     6121 tttgtgtcag ttgccacagt atgcaaccgg tgtatgaaga atataaacag tcggtgcatt
     6181 tccagaacgc ctccggcgtg cgagctgaat gccatgactg tcatatcccg ccggatattc
     6241 caggcatggt gaagcgcaaa ctggaagcga gcaatgatat ctaccagacc tttattgctc
     6301 actccattga tacacctgaa aaattcgaag ccaaacgcgc ggaacttgcc gagcgtgaat
     6361 gggcgcgaat gaaagaaaac aactcggcaa cctgccgctc ctgccataac tacgatgcga
     6421 tggatcatgc gaagcagcat cctgaagcgg cacgtcagat gaaggtggca gcgaaagata
     6481 atcaatcctg catcgactgt cataaaggta ttgcccacca gttaccggat atgagtagcg
     6541 gcttccgtaa gcagttcgat gagctgcgcg ccagtgctaa tgacagtggt gacacgctgt
     6601 actctattga tattaagcca atttatgcgg cgaaaggcga taaagaagcc tctggttctc
     6661 tgctgcctgc ttcggaagtg aaagtcctta aacgtgacgg cgactggctg caaattgaaa
     6721 ttaccggctg gacggaaagc gccggacgtc agcgtgtact cacccaattc ccaggtaaac
     6781 gcatctttgt tgcctcgatt cgtggtgatg tgcagcagca ggtaaaaacg ctggagaaaa
     6841 ccaccgttgc cgacaccaat accgagtgga gcaagttgca ggccactgcg tggatgaaga
     6901 aaggtgatat ggtgaacgat atcaaaccga tctgggctta tgcggattcg ttgtacaacg
     6961 gcacctgtaa ccagtgccac ggcgcaccgg aaatctctca ctttgatgct aacggctgga
     7021 ttggcacgct caacggcatg attggcttta ccagtctcga taaacgtgaa gaacgcacct
     7081 tgttgaaata tctgcaaatg aatgcgtctg acatcgcagg taaggctcac ggcgataaga
     7141 aggaagaaaa ataatgaaca ataacgatct ctttcaggca tcacgtcggc gttttctggc
     7201 acaactcggc ggcttaacag tcgccgggat gctggggccg tcattgttaa cgccgcgcag
     7261 tgcgactgcg gcgcaagcgg cggatgtcat ctcgaaagag ggcattctta ccgggtcgca
     7321 ctggggggct atccgcgcga cggtgaagga tggtcgcttt gtggcggcaa aaccgttcga
     7381 actggataaa tatccgtcga aaatgattgc cggattgccg gattgccgga ttgccggatt
     7441 gccggattgc cggatcacgt acacaacgcg gcgcgtattc gttatccgat ggtacgcgtg
     7501 gactggctgc gtaagcgcca tctcagcgat acctcccagc gcggtgataa ccgttttgtg
     7561 cgcgtgagct gggatgaagc cctcgacatg ttctatgaag aactggaacg cgtgcagaaa
     7621 actcacgggc cgagtgcctt gctgaccgcc agtggttggc aatcgacggg gatgttccat
     7681 aacgcttcgg ggatgctggc gaaagctatt gccttgcatg gtaatagcgt tggtacgggc
     7741 ggagattact ctaccggtgc tgcgcaggtg atcctgccgc gcgtagtcgg ttcgatggaa
     7801 gtgtatgaac agcaaacctc ctggctgctg gtattgcaga acagcaaaac cattatgctg
     7861 tggggctccg atttgctgaa aaaccagcaa gcgaactggt ggtgcccgga tcacgatgtt
     7921 tatgaatatt acgagcaact aaaagcgaaa gtcgccgccg gtgaaattga ggtcatcagc
     7981 atcgatccgg ttgtcacatc cacccatgag tatctggggc gcgagcatgt gaagcacatt
     8041 gcggttaacc cgcaaactga cgtgccgctg caactggcgc tggcgtatac gctgtacagt
     8101 gaaaacctgt acgacaaaaa ctttctcgct aactactgtg taggttttga gcagttcctg
     8161 ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg ccgcatgggc tgaaaaactg
     8221 accggcattg atgccgaaac cattcgtggg ctggcgcggc agatggcggc gaacagaacg
     8281 caaattattg ctggctggtg cgtgcagcgt atgcagcacg gtgaacagtg ggcgtggatg
     8341 attgtggttc tggcggcgat gctggggcaa attggcctgc caggtggtgg ttttggtttt
     8401 ggctggcact acaacggcgc aggcacgccg gggcgtaaag gcgttattct gagtggtttc
     8461 tccggctcta cgtcgattcc gcctgttcac gacaacagtg actacaaagg ctacagcagc
     8521 accattccga ttgcccgttt tatcgatgcg atcctcgaac cggggaaagt gatcaactgg
     8581 aacggtaaat cggtaaaact gccgccactg aaaatgtgta tttttgccgg aactaaccca
     8641 ttccatcgcc atcagcagat caaccgcatt atttaaggct ggcgcaagct ggaaacggtt
     8701 atcgccatag ataaccagtg gacctcaacc tgccgctttg ccgatatcgt actgcctgcg
     8761 accacgcagt ttgagcgtaa cgatctcgac cagtacggca accactccaa ccgtggcatt
     8821 atcgccatga aacaggtggt gccgccgcag ttcgaggcgc gcaacgactt tgatattttc
     8881 cgcgagctgt gccgtcgctt taatcgcgaa gaagccttta ccgaagggct ggacgaaatg
     8941 ggctggctga aacgcatctg gcaggaaggt gtacaacaag gcaaaggacg cggcgttcat
     9001 ctgccagcgt ttgatgactt ctggaataac aaagagtacg tcgagtttga ccatccgcag
     9061 atgtttgttc gccaccaggc attccgcgaa gatccggatc tcgaaccgct gggcacgccg
     9121 agtggcctga ttgagatcta ctcgaaaacc atcgccgata tgaactacga cgattgtcag
     9181 gggcatccga tgtggtttga gaaaatcgaa cgctcccacg gtgggcccgg ctcgcagaag
     9241 tatccgttgc atctgcaatc tgtgcatccg gatttccgac ttcactcgca gttatgtgag
     9301 tcggaaactc tgcgtcagca atatacggta gcgggtaaag agccagtatt cattaacccg
     9361 caggatgcca gcgcgcgcgg tattcgtaac ggtgatgtgg tacgcgtctt taacgctcgc
     9421 ggtcaggtgt tggcaggggc agtagtttct gaccgctatg cacccggcgt ggcacgaatt
     9481 cacgaagggg catggtacga tccagataaa ggcggcgagc ctggtgcgct gtgcaaatac
     9541 ggtaacccca acgtgctgac catcgacatc ggtacttcgc agctggcgca ggcgaccagt
     9601 gcgcacacta cgctggtgga aattgagaag tacaatggag cagtggagca ggtgacggcg
     9661 tttaacggcc ctgtggagat agtggcgcag tgcgaatatg ttcccgcgtc gcaggtgaaa
     9721 tcatgaccac gctgacagca caacagattg cctgtgttta cgcctggctg gcgcagttgt
     9781 tctcccgtga actggacgat gaacaactga cgcaaatcgc cagtgcgcag atggcggaat
     9841 ggttttcgtt gctgaaaagc gaaccgccgc tggctgcggc ggtgaatgag ctggaaaact
     9901 gtattgccac gctgacagta cgtgacgatg cccgtctgga actggccgct gacttttgcg
     9961 gcctgttttt gatgactgat aagcaggcgg cgctgccgta tgcatcggcc tacaaacagg
    10021 acgagcaaga gattaaacgc ttgttagttg aggccgggat ggaaaccagc ggcaatttca
    10081 acgaaccggc agaccatctg gcgatctatc tcgagttgtt gagtcatctg catttttcgc
    10141 tgggagaggg gaccgttcct gcgcgaagaa tcgacagttt gcggcaaaaa acactgacgg
    10201 cgctgtggca atggttacca gagtttgctg cgcgttgcca tcagtatgac agctttggtt
    10261 tttacgcggc actaagccag ttattgctgg tgttagtgga gtgcgaccac caaaacaaat
    10321 aacgtcgttt atgcgcctga aaagacgcgt ttagcgtcgc atcaggcatt atggcgcagt
    10381 tgccggatgc gtcatcacat ccggcaaaca cacatcacgg atgagctaca aaccgggaaa
    10441 gccgctggcg tagcaggcgg ttttcctgct tcaggtgtgc aatatcatcc attaacgtca
    10501 gcgccaccgc gatccccggc cagtccagag ccagttcatg acgcaggcgt accgcgcgtt
    10561 gcaccacaat ggcggcatgg tcgtcaaata cccaggttgt ttcctggatc tcacgcggtt
    10621 caaccacccc caaaccgaca atttcattca actcctcttc agagatgccg gtatgcaggc
    10681 aaaattcggt aatagtaaaa gtcaccgtaa cattagccat catgctttcc cccaatcttt
    10741 acgtggatca aaagacgact gggcgtctgc cagttgctgc cacagcgcgg cagtgttttc
    10801 atccggtttc ggcggcatca cgattttcag taccgcatac agatcgccgg tctgtttttt
    10861 gctcaccaga cctttgcctt taacgcgcaa tcgttgcccg gcctggctgc ctggcgggat
    10921 agtcagcaaa atgctttctt tcagtgttgg aacggtgact ttagcaccca gcgccgcttc
    10981 ccacgggcta accggcacca caatttccag atcctggccg acaatatcga acagcggatg
    11041 tggcgcaata tgaatcacca gccacaaatc gccatttgga ccaccgtttt cgcccggcgt
    11101 cccctggcct ttcagacgga tgcgttgacc attgccgacg cccgccggga tcttcacatt
    11161 cagcgttttc gggatttcct gttcgatcat gccaaaggcg ttataaaccg gcaggttata
    11221 gctgatggta cgcttatgct cagtcagcgt ttcttcgagg aataccgcca cttcgatttc
    11281 aatatcgtgg ccgcgtgcgg cggggcgttg acggctctgg cgggcatgct gaccgaaaat
    11341 tgacgagaag atatcgtcaa aatcttcggc attaaaactc tgaccgtcgc catgatggaa
    11401 ctgacggtta aattgcggat cgttgcgatg ttgccacatc tgatcat
//
LOCUS       DAJTYD010000122        11365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTYD010000122 DAJTYD010000000
VERSION     DAJTYD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.122"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..391)
                     /gene="ychE"
                     /locus_tag="OO585_002909"
     CDS             complement(<1..391)
                     /gene="ychE"
                     /locus_tag="OO585_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6630432.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAG"
     gene            869..3544
                     /gene="adhE"
                     /locus_tag="OO585_002910"
                     /gene_synonym="adhC"
     CDS             869..3544
                     /gene="adhE"
                     /locus_tag="OO585_002910"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6630433.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3668..4564
                     /locus_tag="OO585_002911"
                     /pseudo
     CDS             3668..4564
                     /locus_tag="OO585_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(4846..5463)
                     /gene="tdk"
                     /locus_tag="OO585_002912"
     CDS             complement(4846..5463)
                     /gene="tdk"
                     /locus_tag="OO585_002912"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6630434.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6068..6481
                     /gene="hns"
                     /locus_tag="OO585_002913"
     CDS             6068..6481
                     /gene="hns"
                     /locus_tag="OO585_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6630435.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6625..7533)
                     /gene="galU"
                     /locus_tag="OO585_002914"
     CDS             complement(6625..7533)
                     /gene="galU"
                     /locus_tag="OO585_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6630436.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7735..8748)
                     /gene="rssB"
                     /locus_tag="OO585_002915"
     CDS             complement(7735..8748)
                     /gene="rssB"
                     /locus_tag="OO585_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6630437.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8840..9745)
                     /gene="rssA"
                     /locus_tag="OO585_002916"
     CDS             complement(8840..9745)
                     /gene="rssA"
                     /locus_tag="OO585_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6630438.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRVLGADIVIAVDLQHDAHLIQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            9858..10316
                     /locus_tag="OO585_002917"
     CDS             9858..10316
                     /locus_tag="OO585_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6630439.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10366..11208
                     /gene="purU"
                     /locus_tag="OO585_002918"
     CDS             10366..11208
                     /gene="purU"
                     /locus_tag="OO585_002918"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6630440.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
BASE COUNT         3045 a         2833 c         2580 g         2907 t
ORIGIN      
        1 gccccgccat caacggcaac gccaatggca ccacaccaat gctttcacgt accgcggttt
       61 ctgatttttc ttgcttgttc tgtttatcct cgccaagctt gccgctgatc atcgacatcg
      121 ctatcgtcac caccaggatg cccccggcga tacggaacga atcaattgat ataccaaaaa
      181 gttgcagaat cgtgtcgccg agaaaaagcg agatccacaa gataatggcc acagacaggt
      241 tggctgtaag gttagttttg tttcgcgctg ccgctgtctg ataactggtc atgctgataa
      301 agacgggaat aatccctacc gggttgacca gcgcaaataa cccgatgaaa aatttgaagt
      361 aaacgggaaa atcaaaaaag gtctgaatca cggttagctc cgaagcaaaa gccggataat
      421 gttagccata aataaggttg aaaagacgcg ctgacaatac gccttttgac agcatttttc
      481 acctcctaac tacttaaaat tgctatcatt cgttattgtt atctagttgt gcaaaacatg
      541 ctaatgtagc caccaaatca tactacaatt tattaactgt tagctataat ggcgaaaagc
      601 gatgctgaaa ggtgtcagct ttgcaaaaaa tttgatttgg atcacgtaat cagtacccag
      661 aagtgagtaa tcttgcttac gccacctgga agtgacgcat tagagataat aactctaatg
      721 tttaaactct tttagtaaat cacagtgagt gtgagcgcga gtaagctttt gattttcata
      781 ggttaagcaa atcatcaccg cactgactat actctcgtat tcgagcagat gatttactaa
      841 aaaagtttaa cattatcagg agagcattat ggctgttact aatgtcgctg aacttaacgc
      901 actcgtagag cgtgtaaaga aagcccagcg tgaatatgcc agtttcactc aagagcaagt
      961 agacaaaatc ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc cactcgcgaa
     1021 aatggccgtt gccgaatccg gcatgggtat cgtcgaagat aaagtgatca aaaaccactt
     1081 tgcttctgaa tatatctaca acgcctataa agatgaaaaa acctgtggta ttctgtctga
     1141 agacgacact tttggtacca tcactatcgc tgaaccaatc ggtattattt gcggtatcgt
     1201 tccgaccact aacccgactt caactgctat cttcaaatcg ctgatcagtc tgaagacccg
     1261 taacgccatt atcttctccc cgcacccgcg tgcaaaagat gccaccaaca aagcggctga
     1321 tatcgttctg caggctgcta tcgctgccgg tgctccgaaa gatctgatcg gctggatcga
     1381 tcaaccttct gttgaactgt ctaacgcact gatgcaccac ccagacatca acctgatcct
     1441 cgcgactggt ggtccgggca tggttaaagc cgcatacagc tccggtaaac cagctatcgg
     1501 tgtaggcgcg ggcaacactc cagttgttat cgatgaaact gctgatatca aacgtgcagt
     1561 tgcatctgta ctgatgtcca aaaccttcga caacggcgta atctgtgctt ctgaacagtc
     1621 tgttgttgtt gttgactctg tttatgacgc tgtacgtgaa cgttttgcaa cccacggcgg
     1681 ctatctgttg cagggtaaag agctgaaagc tgttcaggat gttatcctga aaaacggtgc
     1741 gctgaacgcg gctatcgttg gtcagccagc ctataaaatt gctgaactgg caggcttctc
     1801 tgtaccagaa aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg aaagcgaacc
     1861 gttcgcacat gaaaaactgt ccccgactct ggcaatgtac cgcgctaaag atttcgaaga
     1921 cgcggtagaa aaagcagaga aactggttgc tatgggcggt atcggtcata cctcttgcct
     1981 gtacactgac caggataacc aaccggctcg cgtttcttac ttcggtcaga aaatgaaaac
     2041 ggctcgtatc ctgattaaca ccccagcgtc tcagggtggt atcggtgacc tgtataactt
     2101 caaactcgca ccttccctga ctctgggttg tggttcttgg ggtggtaact ccatctctga
     2161 aaacgttggt ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag ctgaaaacat
     2221 gttgtggcac aaacttccga aatctatcta cttccgccgt ggctccctgc caatcgcgct
     2281 ggatgaagtg attactgatg gccacaaacg tgcgctcatc gtgactgacc gcttcctgtt
     2341 caacaatggt tatgctgatc agatcacttc cgtactgaaa gcagcaggcg ttgaaactga
     2401 agtcttcttc gaagtagaag cggacccgac cctgagcatc gttcgtaaag gtgcagaact
     2461 ggcaaactcc ttcaaaccag acgtgattat cgcgctgggt ggtggttccc cgatggacgc
     2521 cgcgaagatc atgtgggtta tgtacgaaca tccggaaact cacttcgaag agctggcgct
     2581 gcgctttatg gatatccgta aacgtatcta caagttcccg aaaatgggcg tgaaagcgaa
     2641 aatgatcgct gtcaccacca cttctggtac aggttctgaa gtcactccgt ttgcggttgt
     2701 aactgacgac gctactggtc agaaatatcc gctggcagac tatgcgctga ctccggatat
     2761 ggcgattgtc gacgccaacc tggttatgga catgccgaag tccctgtgtg ctttcggtgg
     2821 tctggacgca gtaactcacg ccatggaagc ttatgtttct gtactggcat ctgagttctc
     2881 tgatggtcag gctctgcagg cactgaaact gctgaaagaa tatctgccag cgtcctacca
     2941 cgaagggtct aaaaatccgg tagcgcgtga acgtgttcac agtgcagcga ctatcgcggg
     3001 tatcgcgttt gcgaacgcct tcctgggtgt atgtcactca atggcgcaca aactgggttc
     3061 ccagttccat attccgcacg gtctggcaaa cgccctgctg atttgtaacg ttattcgcta
     3121 caacgcgaac gacaacccga ccaagcagac tgcattcagc cagtatgacc gtccgcaggc
     3181 tcgccgtcgt tatgctgaaa ttgctgacca cctgggtctg agcgcaccgg gcgaccgtac
     3241 tgctgctaag atcgagaaac tgctggcatg gctggaaacg ctgaaagctg aactgggtat
     3301 tccgaaatct atccgtgaag ctggcgttca ggaagcagac ttcctggcga acgtggataa
     3361 actgtctgaa gatgcattcg atgaccagtg caccggcgct aacccgcgtt acccgctgat
     3421 ctccgagctg aaacagattc tgctggatac ctactacggt cgtgattatg tagaaggtga
     3481 aactgcagcg aagaaagaag ctgctccggc taaagctgag aaaaaagcga aaaaatccgc
     3541 ttaatcagta gcgctgtctg gcaacataaa cggccccttc tgggcaatgc cgatcagtta
     3601 aggattagtt gaccgatcct taaactgagg cactataacg gcttccacaa cagggagccg
     3661 ttttcttatg ccacttctca atgatctgct cgatttcagt gaccatccgc ttatgcctcc
     3721 gccctctgca caactatttg cagaacacct tcccaccgag tggatacaac actgcctgac
     3781 gctttctgct catgcgaccg ttcgccgccg tcgtttaccg ggggacatgg ttatctggat
     3841 ggtggtgcaa tgagccaatt accgatgttg ttcgccgtct gaacctgagc gcggatggcg
     3901 aagcggggat gaacctgctg gcccgcagcg ctgtcaccca agcgcgtcag cgcgtggggg
     3961 ccgccccagt ggaatggctc ttccgccaga ccgcacagac aggggcgcgg aacgttacct
     4021 gaaggatgac tggcacggcc tgcaactttt tgccattgat ggcgcacagt tcaggacacc
     4081 tgataaaccc gagctgcgtg aatattatgg gtctgccaac acatccacta agcggcagaa
     4141 cgcctacccg gttatgcgtc tggtggcctt aatgaacctg ggaagtcaca ttctgctgaa
     4201 tgccgtgacc gcaccttacc ggcagagcga aactgtgctg gcccactcca tgctcgccac
     4261 catcccggat aactctatta cgttgtttga taagcttttt tacagcgaag acctgctgct
     4321 aacgctgaac cagaagggtt gtaaccgcca ctggctactg cctgcgtgga agaatatcgc
     4381 atcagaaatg atagagctgg gaaacacggc gtcaccaggg accatcccga aacgactgga
     4441 gcatcttcgg ggtgctctgg aagtggtgtt cataacaaaa cgcccccgac catcaaggcc
     4501 gaggtcggtt aagatatcaa aaacccgtta tcctgtgaaa catagtgccg ctcctcttaa
     4561 gtgaacggca ttgcccttct ggggccgtat tttttgttta cccaaagcaa cttttccata
     4621 aaccgacagc attagccttc atcatatttg cgacgatgta taacgcctaa acacagggat
     4681 attgtacttt acaggtcaca agtcaacgtc ggtgcttaag agccctgtga ggcgtatagc
     4741 ggcgttaaaa aactgccgag aagggtatat agcccggaag aagtgcgtaa aacgaactga
     4801 caggataaaa gtgccctgct caccctgtca gtaaagaaat tcttattaat cgtggcgatg
     4861 cctttcctga atagccgtta atgagccgac ttgtaacgcc tctttatagt gtttacggca
     4921 tacagaaacg tatcgttcat taccaccaat taccacctgc tcaccttcgt tataaggtct
     4981 gcctgcttga tcaagacgca gcaccatgct tgctttacgg ccacaaaaac agatggtttt
     5041 taattcaacc agtttgtcgg accatgccag taagtattgg ctgccaataa ataattcacc
     5101 tcgaaaatcg gtacgtaaac cataacaaag tacgggtata tcgagttgat cgacaacctc
     5161 cgataattca tatacttgtt gtctggttaa aaactggcat tcatcaacca gtacgcaatg
     5221 aattgcctgc tgttcatgtt ccgcacgaat ctcatcaaat aatgatgaat tttggttaaa
     5281 taattttgca ggcgatgaca aacctatacg cgaactgact ttcccggcac caaagcgatc
     5341 atcaatttct gccgtatata cgacagtgcg catgccgcgt tcttggtaat tgtatgaaga
     5401 ttgcaacaat gctgtagact tacccgcatt cattgcggaa tagtagaaat atagctgtgc
     5461 catcagccac aggccctcaa tgatatgaaa taaagttggc tggagtttat cataattcgt
     5521 cagtttttca gctcaacagt atgcacagaa gtgggtatat gcgttctccc ttacgaagcc
     5581 ttgcataatc cttctgagct atcattacaa ctgcctcgct tgttataagc gggtaaatga
     5641 ctgctggtaa ctattcacaa tctttaacct gttgcgcaag taatagccct ctgttgacct
     5701 ccaggagata gtgcaatact aagtccatgc tcttattgcg actgttctac ttttcatcat
     5761 tcgcttaata gggaattctc gtaaacacaa ctaatacaga agactgaaag gccgtcagcc
     5821 tacgataatc tccccataaa atgtgacatg aatcaggaag ttttaacctc acgtgctgcg
     5881 aaatcatcgg tgtaaatagg gctatatgcc gcgtcttttc tggctaattt tatgaaaaga
     5941 tatttattgg cggcacaaaa taaagaacaa ttttgaattc cttacattcc tggctattgc
     6001 acaactgaat ttaaggctct attattacct caacaaacca ccccaatata agtttgagat
     6061 tactacaatg agcgaagcac ttaaaattct gaacaacatc cgtactcttc gtgcgcaggc
     6121 aagagaatgt acacttgaaa cgctggaaga aatgctggaa aaattagaag ttgttgttaa
     6181 cgaacgtcgc gaagaagaaa gcgcggctgc tgctgaagtt gaagagcgca ctcgtaaact
     6241 gcagcaatat cgcgaaatgc tgatcgctga cggtattgac ccgaacgaac tgctgaatag
     6301 ccttgctgcc gttaaatccg gcaccaaagc taaacgtgct cagcgtccgg caaaatatag
     6361 ctatgttgac gaaaacggcg aaactaaaac ctggactggc cagggccgta ctccagctgt
     6421 aatcaaaaaa gcaatggatg agcaaggtaa atccctcgac gatttcctga tcaagcaata
     6481 atcttttgta gattgcaatt gctaaaaatc ccgccatcgg cgggattttt tattgtccgg
     6541 tttaagacaa tttaataaga ataagctata aaaaaacggc gtcgattgtt caacgccgtt
     6601 tcgtggataa caccgatatg gatgttactt cttaatgccc atctcttctt caagccaggc
     6661 tttaaattcc gtgccaaggg tgttatgacg aataccgtat tcaacgaagg cctgcatgta
     6721 acctaattta ttaccgcagt catggctctt ccctttcata tgataggctt ccaccgtttc
     6781 tttttcgatc agcatatcaa ttgcgtcggt gagctgaatt tcatcaccag ctcccggagg
     6841 ggtttttgcc agcaacggcc aaatatccgc gctaagtacg taacgaccca caatagcgag
     6901 attagacggc gcaacatccg ctttcggttt ttctaccaca ccaaccatcg gtacactttc
     6961 acccggcgct aattcaacgc ctttgcaatc cacaacgcca tatgcggtca catcagcaac
     7021 ctgttcaacc atgatctggc tatgacccgt ttcatcaaag cggcggatca tctctgccag
     7081 gttatcctgt gacaaatcgg attcatattc atccagaata acatcaggca aaataacagc
     7141 taccggttca tcacccacta ccgggtgagc acacaatacc gcgtgtccca ggcctttcgc
     7201 cagcccctga cgaacttgca taatagtcac gtgcggcgga caaatagact gcacttcatc
     7261 aagcagttga cgttttacac gtttttccag cattgcttcc agttcaaaac tggtatcaaa
     7321 gtggttttca atcgagtttt tagatgagtg cgtaaccagc acaatttcag taatgccagc
     7381 cgcaatacat tcattcacga cgtattgaat taatggctta tcgacaagtg gcagcatctc
     7441 tttcgggatg gctttcgtcg ccggcaacat cctggttcct aatcccgcaa cggggataac
     7501 ggcttttttg actttcgtat taatggcagc catttaaatt ctcctggact gttcgtgttt
     7561 tgaacgtgtt catatttctg tatcgcatcc cagtatatca gcaccccgaa ggggctaagt
     7621 tctgaaaagg aggcgagtta gcataattaa gacaattact tatacgaatg gcgacgatag
     7681 taccaccagc cgacattagc aggtaatgca aatttagtcc gcgttatcgt ttgctcattc
     7741 tgcagacaac atcaagcgca gtcgaccacc ggttccccat atttggcatt gccaggcatc
     7801 gcatcgctgg ctcaattgat tcaaataagc gttacctaaa gtgcctaacg gaacaccatt
     7861 actgatttgc acctggtgtt cgccggtatt taacgtcgca ttcagacccg cagaaaccag
     7921 aatgagattt ttcagttcgc gatgataata gccaactaat agcggaaact gccccggcag
     7981 attggcctga cggagtaaat ggtttacctg cttcaataac gcgcccagct ctggcaaccg
     8041 ttgattttgg tgtgcaagct gttcctgtaa taatccgtta aacaatgcgc gtaataacaa
     8101 ggcagcaagt acgccattat gtccagctcg ggtgacatca aggcaataaa atgccagatc
     8161 gttttccgaa agtgcggcaa tatcaagcac caggccgggt ttgtccgcgg caaccaattg
     8221 acgataatta acccggcaat gggaaatcac ctgctgaacc ggcggttgta gttcctgtaa
     8281 taatttcgcc gctgcggcag ggttatcaac cgttgcatcc caatcgcgaa aaagcctttc
     8341 ctcttcctca acgcgcgaat taaacatgct gggatagaga caggcaaaaa ccatctcgcg
     8401 caagcgattc agatctttaa ctggtttcag caaaacatct tcaacgccca gacgtaacgc
     8461 tttggcaata tctgccatat tttcagtggc agatatcacc agaactgggg tctggtcgcc
     8521 tctgttacgt atatgctcca gcagtttaag cccgttcatt cgtggcatcg cgatatcaca
     8581 tatcatcagg tctggagtga aacctcccag caactcaagg gcatccaccc catcagccgc
     8641 cagtaccgtt gtcgctccca atgaggaaaa ccatgaatcc agaagcgagc gaaatacctg
     8701 ctcatcttca acaatgagaa tctgttttcc gaccaatggc tgcgtcatgt tctctcccct
     8761 gactggcttt actcaatagt ggcatgctat tgccacctgc gcctgtcaga attaacttaa
     8821 gtgtagataa aaattctggt caaatgttgg tgcgtaccaa cggcaaaagt tcgtccattt
     8881 tcctttccac tgccagctgt ccggccgcaa tggcagcgtg cgcgcgatgg aaatcaagcg
     8941 tagatatttg cgggcaaaca ggttgaatca gaatatcggg cggatcacct gccatgcggt
     9001 tccttttaag gcggttctcc agcacctgga tagaagtggt cataatctct gttgccgttg
     9061 gcgctgtcac cgcccgacgt gttgttatac tgcctaacct ttctttcaga cgcgcatgcc
     9121 acggcaaaga ttcaccattc tcgctatttt cttcactgac attaaaggag agcaaatctt
     9181 gttgtatcaa atgagcatcg tgctgcaggt cgaccgctat cacaatatca gcccccaata
     9241 cacgcgtgag ggaaatagga attgggttaa ctactgctcc atcaaccagc cagtagccgt
     9301 tatgtgcaac aggtgccatg agtcctggaa tactgcatga tgcgcgaata gcaagatgga
     9361 gatcgccttc agtaaaccat aattcacgtc ccgtacttaa attggtggca acagccgcaa
     9421 agcgacggga acaattttcg atctctgttt ccggcattat ttcgcggcat tgattgaaga
     9481 cacgctcgcc gcgcagtaac ccaccgcgct gccaggagag atccatcagg cgtaaaacat
     9541 cccaataact gaaagaggtc acccaatctt ccagcgcaga taatcgatcg catgcatagg
     9601 cagcgcccac cagcgaacca attgaacatc ctgcaacgat atcaatttca atacccactt
     9661 tttttagcgc attaataacg ccaatatgcg accaacctct cgccgcgcca gatcccagcg
     9721 ccagccctat ttttatcttt ctcattattc ccgcaagatt cccctggaat gcaatcgtag
     9781 ccacatcgct actgctcggt taacatagtg ctactcaggc ggttaatacg ccgttattgt
     9841 ttccagggag attatttgtg tctcagcttt gtccctgtgg tagtgctgtc gagtatagcc
     9901 tatgttgcca cccttatgtg tctggtgaaa aggttgcacc tgatccagaa catctcatgc
     9961 gttcgcgtta ctgcgctttt gtgatgcaag acgcagatta tttaataaag acctggcatc
    10021 cctcttgtgg agcagcggcg ttacgtgccg aattgatggc cggatttgcg catactgaat
    10081 ggttgggatt gaccgtattt gaacattgtt ggcaggacgc cgacaatatc ggatttgtca
    10141 gttttgttgc tcgatttacc gaaggtggca aaacaggtgc aataattgaa cgttcgcgat
    10201 ttttgaaaga aaacggtcaa tggtactata ttgacggtac acgtccgcag tttggtcgca
    10261 acgatccctg cccttgtggt tcaggtaaaa aatttaaaaa gtgctgcggc caataatggt
    10321 tgacggtacg gtttagcaaa cactctcaac aaggtttttc cagcaatgca ttcactccaa
    10381 cgtaaagttc tgcgtactat ttgtccggac caaaaaggtc tgatcgcacg tattaccaat
    10441 atttgctaca agcacgagtt aaatatcgta cagaacaatg aatttgttga tcaccgtacc
    10501 gggcgctttt ttatgcgcac ggaactggaa gggattttta atgattccac cctgctggcg
    10561 gatctcgata gcgcattgcc agaaggctcc gtgcgtgagc tgaatcctgc cggtcgtcgc
    10621 cggatagtga ttctggtcac taaagaagcg cattgccttg gcgatttgtt gatgaaagcc
    10681 aattacggcg gcctggatgt cgaaatcgcg gcagttattg gtaaccacga tactttacgt
    10741 tctctggttg agcgttttga tattccgttt gagctggtaa accatgaagg gttaagccgc
    10801 aacgagcacg atcaaaagat ggcagacgcc attgatgctt ataaacctga ctatgtggtg
    10861 ctggcgaagt atatgcgggt attaacgccg gaatttgtgg cacgcttccc gaataaaatc
    10921 atcaatattc accattcatt cctgcctgcg tttatcggcg cacgtcctta tcaccaggcc
    10981 tatgaacgtg gtgtgaagat tattggcgca accgctcact atgtgaatga caatctggac
    11041 gaaggcccaa tcatcatgca ggacgttatt catgtcgatc atacctacac agctgaagat
    11101 atgatgcgcg caggtcgtga cgtcgagaaa aacgtcttaa gtcgtgcgct atacaaagta
    11161 ctggcacagc gcgtctttgt ttacggtaat cggacgatta ttctttaatc gccagcaaaa
    11221 ataactggtt acctttaatc cgttacggat gaaaattacg caaccagttc atttttctca
    11281 acgtaacact ttacagcggc gcgtcatttg atatgatgcg ccccgcttcc cgataaggga
    11341 gcaggccagt aaaaagcatt acccc
//
LOCUS       DAJTYD010000123        11360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTYD010000123 DAJTYD010000000
VERSION     DAJTYD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.123"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            221..1261
                     /gene="gpr"
                     /locus_tag="OO585_002919"
     CDS             221..1261
                     /gene="gpr"
                     /locus_tag="OO585_002919"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6630441.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1301..1795)
                     /locus_tag="OO585_002920"
     CDS             complement(1301..1795)
                     /locus_tag="OO585_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6630442.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            1986..2870
                     /gene="yghA"
                     /locus_tag="OO585_002921"
     CDS             1986..2870
                     /gene="yghA"
                     /locus_tag="OO585_002921"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6630443.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3142..3567)
                     /gene="exbD"
                     /locus_tag="OO585_002922"
     CDS             complement(3142..3567)
                     /gene="exbD"
                     /locus_tag="OO585_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6630444.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDLVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3574..4308)
                     /gene="exbB"
                     /locus_tag="OO585_002923"
     CDS             complement(3574..4308)
                     /gene="exbB"
                     /locus_tag="OO585_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6630445.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4560..5747
                     /gene="metC"
                     /locus_tag="OO585_002924"
     CDS             4560..5747
                     /gene="metC"
                     /locus_tag="OO585_002924"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6630446.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            5887..6546
                     /locus_tag="OO585_002925"
     CDS             5887..6546
                     /locus_tag="OO585_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6630447.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6586..7485)
                     /gene="yqhC"
                     /locus_tag="OO585_002926"
     CDS             complement(6586..7485)
                     /gene="yqhC"
                     /locus_tag="OO585_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6630448.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            7679..8842
                     /gene="yqhD"
                     /locus_tag="OO585_002927"
     CDS             7679..8842
                     /gene="yqhD"
                     /locus_tag="OO585_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6630449.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            8947..9774
                     /gene="dkgA"
                     /locus_tag="OO585_002928"
     CDS             8947..9774
                     /gene="dkgA"
                     /locus_tag="OO585_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6630450.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            9974..10900
                     /locus_tag="OO585_002929"
     CDS             9974..10900
                     /locus_tag="OO585_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6630451.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            10951..11208
                     /gene="yqhH"
                     /locus_tag="OO585_002930"
     CDS             10951..11208
                     /gene="yqhH"
                     /locus_tag="OO585_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6630452.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
BASE COUNT         2934 a         2874 c         2854 g         2698 t
ORIGIN      
        1 tgagcgtaat agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc
       61 ggcatcgttg cattcctcaa ctggtgtttt ttaagcaaag cataagcgcg tgtttttgcc
      121 cagtttttcg tcactctgtg agccagacta cgggatacgc gctggcgaat cgctaaacta
      181 gaaacattgt ttcgaaattg aacggtggaa aggagaggtc atggtctggt tagcgaatcc
      241 cgaacgttac gggcagatgc agtaccgcta ttgcggaaaa agtggtttac gcctgcccgc
      301 gttatcgctc ggtttatggc acaatttcgg tcacgttaac gcgctggaat cacagcgtgc
      361 aatcctgcgt aaagcgtttg atttaggcat tacgcacttt gatttagcca acaattacgg
      421 gccgcctcca ggaagcgcag aagagaactt tggtcgcctg ctgcgggagg attttgccgc
      481 ttatcgcgat gaactgatta tctctaccaa ggctggctac gatatgtggc ccggccctta
      541 cggctctggc ggttcacgta aatacctgct cgccagcctc gaccaaagcc tgaagcgtat
      601 ggggcttgag tatgtcgata tcttttactc tcatcgcgtc gatgaaaata cgccgatgga
      661 agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt aaggcgctgt atgtcgggat
      721 ctcctcttac tcgccagagc ggacgcaaaa aatggtcgag ttgctgcacg agtggaaaat
      781 tccgctttta attcatcaac cttcgtacaa tttactgaac cgctgggtgg ataaaagcgg
      841 cctgctggat accctgcaaa ataacggcgt gggctgtatt gcctttactc ctctggctca
      901 gggattgctg accggaaaat atctcaacgg cattccgcaa gattcacgga tgcatcgtga
      961 agggaataaa gttcgtggtc tgacgccgaa aatgcttacc gaagccaacc tcaacagcct
     1021 gcgcttattg aatgaaatgg cacagcagcg tggacaatca atggcgcaaa tggcgttaag
     1081 ctggttgctg aaagatgatc gcgtgacgtc ggtattgatt ggtgtcagcc gcgcggagca
     1141 actagaggag aacgtgcagg cgctgaataa tctgacattt agcaccgagg agctggcgca
     1201 gattgatcag catatcgccg atggcgagct gaatctgtgg caggcgtctt ccgataaatg
     1261 acctgttaat aacgggcgcg cgaaccgcgc ccgaatcaga tcagtgatta tgacgagtca
     1321 gtcggtcaag atagcccatc acaaatgcag agagcacaaa cgtcagatgg ataatgacgt
     1381 accacatcag tttgttatcc gggacatttt tcgcatccat aaagacgcgc agtaagtgaa
     1441 tggaagaaat tgccacaatc gacgctgcta ctttgttttt cagcgacgtt gcgtccattt
     1501 tccccagcca gttcagcttc tctttgttct cggagatatc cagctgcgag acgaaattct
     1561 cataaccgga aaacatcacc atcaccagca aaccgccaac cagcgtcata tccaccagcg
     1621 acagcaacac gaggatcaaa tctgattccg ccatcgagaa gatattcggc agtacgtgaa
     1681 taatctcctg gaagaacttc agcgccaggg caactaacgc cagcgaaagg ccaaagtaca
     1741 cgggggcaag cagccagcga gaagcataca ttgcattttc aagaaaacgt tccatacatt
     1801 cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt gagtaaacag cacgcaacag
     1861 caaatcaaca tatacgttaa acctttgtcg cataattctg aggtttaggg atcttcctgg
     1921 ttcccttatc aacgcatcga gagcgtctac ccttaaaaga cgttgaacct gaagggagaa
     1981 aaacaatgtc tcatttaaaa gacccgacta cgcagtatta cactggtgaa tatcccaaac
     2041 agaaacaacc gacgccaggc atccaggcga agatgacacc ggtaccggat tgcggcgaga
     2101 aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc actggtgaca gggggcgatt
     2161 ccggaatagg tcgcgctgcc gccatcgctt acgcgcgtga aggggctgac gtggcgatca
     2221 gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa aaagatcatt gaagaatgcg
     2281 gacgcaaagc cgttctgctg ccaggcgatt taagcgatga gaaattcgcc cgttcgctgg
     2341 ttcacgaagc gcacaaggcg ttaggcgggc tggatattat ggcgctggtc gccgggaaac
     2401 aggttgccat tccggatatt gcagacctca ccagcgaaca gtttcaaaag acctttgcca
     2461 ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat ccccctgcta ccgaaaggtg
     2521 caagtatcat caccacttcg tcaatccagg cataccagcc aagtccgcat ttactggact
     2581 atgcggctac gaaggcggcg attctgaact acagccgtgg cctggcaaaa caggtcgcgg
     2641 agaaaggtat tcgggtgaat attgtcgcgc caggccctat ctggacggca ctgcaaattt
     2701 ccggcggaca aacgcaggat aagatcccgc agtttggtca gcaaacgccg atgaagcgtg
     2761 cggggcaacc ggcggaactg gcccctgtat atgtttatct ggcaagtcag gagtcgagct
     2821 acgtcaccgc agaagtgcac ggtgtgtgcg gcggcgagca tttaggttaa aaaaatgccc
     2881 ggttgtgaaa agcaaccggg catcattgtg aattcactct tacctgatgt ggcgaaaacg
     2941 ccttatccgc ctacaaacgc atgaagattc aacatgttgc aatagatttt gtaggcctga
     3001 taagcgtagc gcatcaggca ttgagcacct aatgccggat gcggcgtgaa cgccttatcc
     3061 ggcctacaaa cgcacgaaga ttcaacatgt tgcaatagat tttgtaggcc tgatgagcgt
     3121 agcgcatcag gcaattctac tttacttcgc tttggcggtt tcttcgccga ccagacctat
     3181 cttcaggtaa cccgcctgat gcagcgtatc cattaccttc atcaacgtct cgtaatcgac
     3241 ggttttatcc gctcggaaga agatggtggt gtctttcttg ccttcggtta acgcattcaa
     3301 cgccgtaatc attgtttcat cggtgaccag atcgttaccg ataaacatcg agttgtctgc
     3361 cttcaccgac agataaaccg gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg
     3421 caagttcacc ttcacatcta ccgtcgctaa cggtgccgcc accataaaga taatcagcag
     3481 aaccaacatc acgtcgataa acggcgtcac gttgatatca tgcatttcgc cgttatcgtc
     3541 gaggttttcg ttaagatgca ttgccatcgg atattatcct gcgcgtaatt tttgtgcgac
     3601 acgaaccgga tgcgcagcgg cgctggcttc cagatccagg tcacggcttt gcagcaacaa
     3661 tacctgcgct gcaacatcac ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac
     3721 gttatagata acgaccgcag gaatcgctgc cacgaggccg attgccgttg ctaacagagc
     3781 ttctgcgata cccggcgcaa cgactgccag gttagtggtc tgcgtttgcg cgataccaat
     3841 aaagctgttc atgatgcccc agaccgtacc aaacagacca acgaacggag aaatcgcgcc
     3901 gatggttgcc agatagccgt taccgcggcc catttgacga cccactgcgg cgacccgacg
     3961 ctccagacgg aagctggtac gttctttaat accttcgtta tcgtcgctgc cttctgacag
     4021 ttccagctca ttctgcgctt cattgaacaa atgcaggctt aagcttttgc taccaaaatc
     4081 agcggcgata tcgttggcct ggtttaagga acgcgcttca gccagcagtt gctgctcgcg
     4141 ctttaggcga cgcttctgat tgaagaactc tacgctctta ctgaagaaga ttgcccaggt
     4201 gactacggag gccaaaataa gcccaatcat cacgcactta acgacaatat cggcgtgctg
     4261 atacataccc cagacggaaa ggtctgtctg cattaaatta ttacccactg tgtatctcca
     4321 ggacgcaagt cacaaaatct gcgcataata atatcaaaac gacgtcgaat tgatagtcat
     4381 tctcattact atttgcatac tgccgtacct ttgctttctt ttccttgcgt ttacgcagta
     4441 aaaaagtcac cagcacgcca tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg
     4501 cgcccgcgaa tattcatgct agtttagaca tccagacgta taaaaacagg aatcccgaca
     4561 tggcggacaa aaagcttgat actcaactgg tgaatgcagg acgcagcaaa aaatacactc
     4621 ttggcgcggt aaatagcgtg attcagcgcg cttcttcgct ggtatttgag agtatggaag
     4681 ccaaaaagca cgcgacgcgc aatcgcgcca atggcgagtt gttctatgga cggcgcggaa
     4741 cgttaaccca tttttcctta caacaagcga tgtgtgaact ggaaggtggc gcaggctgcg
     4801 cgctatttcc ctgcggggcg gcagcggttg ctaattcgat tcttgctttt gtcgaacagg
     4861 gcgatcatgt gttgatgacc aacactgcct atgaaccgag tcaggatttc tgtagcaaaa
     4921 tcctcagcaa actgggcgta acgacatcgt ggtttgatcc gctgattggt gccgatatcg
     4981 ttaagcatct gcagccaaac actaaaatcg tgtttctgga atcgccaggc tccatcacca
     5041 tggaagtcca cgacgttccg gcgattgttg ccgccgtacg cagtgtggtg ccggatgcca
     5101 tcattatgat cgacaacacc tgggcagccg gtgtgctgtt taaggcactg gattttggca
     5161 tcgatgtttc tattcaagcc gccaccaaat atctggttgg gcattcagat gcgatgattg
     5221 gcactgccgt gtgcaatgcc cgttgctggg agcagctacg ggaaaacgcc tatctgatgg
     5281 gccagatggt cgatgccgat accgcctata taaccagccg tggcctgcgc acattaggtg
     5341 tgcgtttgcg tcaacatcat gaaagcagtc tgaaagtggc tgaatggctg gcagaacatc
     5401 cgcaagtagc gcgagttaac caccctgctc tgcctggcag taaaggacac gaattctgga
     5461 aacgagactt tacaggtagc agcgggctgt tttcctttgt gcttaagaaa aaactcagta
     5521 atgaagagct ggcgaactat ctggataact tcagtttatt cagcatggcc tactcgtggg
     5581 gcgggtatga atcgttgatc ctggcgaatc aaccagaaca tatcgccgcc attcgcccac
     5641 aaggcgagat cgattttagc gggaccttga ttcgcctgca tattggtctg gaagatgtcg
     5701 acgatctgat tgccgatctg gacgccggtt ttgcgcgaat tgtataacat tgccactttt
     5761 ggacaatttt gcagacattt aattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg
     5821 cgtcccggac gaatcaggag tacaataggc agataaaggc ttaaacgctg ttccacagga
     5881 aagtccatgg ctgttattca agatatcatc gctgcgctct ggcaacacga ctttgccgcg
     5941 ctggcggatc ctcatattgt tagcgttgtt tactttgtca tgtttgccac gctgttttta
     6001 gaaaacggcc tgctgcccgc ctcatttttg ccaggcgaca gcttgttgat actggcaggc
     6061 gcattgattg cccagggggt tatggatttt ctgcctacga ttgcgattct gaccgccgca
     6121 gcaagtctgg gctgctggct aagttatatt caggggcgct ggttagggaa taccaaaacg
     6181 gtgaaaggct ggctggcaca gcttcctgct aaatatcacc agcgcgccac ctgcatgttt
     6241 gaccgccacg gtctgctggc gctgctggct ggacgttttc ttgcatttgt ccgtacgctg
     6301 ctgccaacca tggcgggaat ttccggtctg ccaaaccgcc gcttccagtt tttcaactgg
     6361 ttaagtggat tgctgtgggt cagcgtggta accagttttg gctatgcctt aagtatgatt
     6421 ccgttcgtta aacgccatga agatcaggta atgacgttcc tgatgatcct gccaattgcc
     6481 ttgttaaccg ctggcttgtt aggcatgctg tttgtggtga ttaaaaaaaa atactgtaac
     6541 gcctgacgat tttccccgtt cccggctgct gtaccgggaa cgtatttaat tcccctgcat
     6601 cgcccgcatt cttgccgcat cttcccccgg cgtcacaccg aagtaacgtt taaactcacg
     6661 gctaaattgc gatgcgcttt catagccgac gcgcatcgct gctgcgctgg ccttcatgcc
     6721 gtcatggatg atcatcatcc gcgccttatg cagacggtaa ttcttcaaat actgcaacgg
     6781 cgaggtgctg gtgacagact taaaattatg gtggaacgcc gatacgctca tgttggcttc
     6841 tgccgccagt tgctcgacgc tcaggttttc ggtgtattta ttctcaatcc gtttcagcac
     6901 gcggctaatc agactgaagt gagtctggcg actgaccagc gccagtaacg cgccgccgca
     6961 aggtccggtc agcacgtagt acagaatttc gcggatgatc tgtttgccga gaatacgcgc
     7021 atccagtggt cgctccatca cgtcgagtaa ccgctccgcc gcgcataaaa tctcttctga
     7081 taacgtggcg gagttaatcc cgctggctgc catcgacggc tggaaatgct catcttcgcc
     7141 aatgtccatc aacagttcct gtaactgcaa aatatcgaca ttgagacgca accctgccag
     7201 cggcacctct gacgtggcat aggtttcgca ctcaaacggc aacggcaccg tcagcagcag
     7261 gtattcattg gcatcataac gaaacacgcg ttcattgata taaccgattt tatgcccgga
     7321 aaagagaatt atgatgccag gctcgtacat caccggtgta cgtgcgaaag gcgtctcacc
     7381 atacaacaaa cgcacatcgg gcaacagttc tgacaaacta ttttctttat ttttcagttt
     7441 attaacttta tccgccagca agcggcaaat ctcttcacgt ttcatatcgc gtaatttctt
     7501 aggaataatg cggcaatttg attgtgcgca attttgtagc atttctccag cactctggag
     7561 aaataggcaa gacattggca gaaatgagca ttgagagcca gggcgctggc gatcacaatg
     7621 aaaaacatca ggcagatcgt tctctgccct catattggcc cagcaaaggg agcaagtaat
     7681 gaacaacttt aatctgcaca ccccaacccg cattctgttt ggtaaaggcg caatcgctgg
     7741 tttacgcgaa caaattcctc acgatgctcg cgtattgatt acctacggtg gcggcagcgt
     7801 gaaaaaaacc ggcgttctcg atcaagttct ggatgccctg aaaggcatgg acgtgctgga
     7861 atttggcggt attgagccaa acccggctta tgaaacgctg atgaacgccg tgaaactggt
     7921 tcgcgaacag aaagtgactt tcctgctggc ggttggcggc ggttctgtac tggacggcac
     7981 caaatttatc gccgcagcgg ataactatcc ggaaaatatc gatccgtggc acattctgca
     8041 aacgggcggt aaagagatta aaagcgccat cccgatgggc tgtgtgctga cgctgccagc
     8101 aaccggttca gaatccaacg caggcgcggt gatctcccgt aaaaccactg gcgacaagca
     8161 ggcgttccat tctgcccatg ttcagccggt atttgccgtg ctcgatccgg tttataccta
     8221 caccctgccg ccgcgtcagg tggctaacgg cgtagtggac gcctttgtac acaccgtgga
     8281 acagtatgtt accaaaccgg ttgatgccaa aattcaggac cgtttcgcag aaggcatttt
     8341 gctgacgctg atcgaagatg gtccgaaagc cctgaaagag ccagaaaact acgatgtgcg
     8401 cgccaacgtc atgtgggcgg cgacgcaggc gctgaacggt ttgattggcg ctggcgtacc
     8461 gcaggactgg gcaacgcata tgctgggcca cgaactgact gcgatgcacg gtctggatca
     8521 cgcgcaaaca ctggctatcg tcctgcctgc actgtggaat gaaaaacgcg ataccaagcg
     8581 cgctaagctg ctgcaatatg ctgaacgcgt ctggaacatc actgaaggtt ccgacgatga
     8641 gcgtattgac gccgcgattg ccgcaacccg caatttcttt gagcaattag gcgtgccgac
     8701 ccacctctcc gactacggtc tggacggcag ctccatcccg gctttgctga aaaaactgga
     8761 agagcacggc atgacccaac tgggcgaaaa tcatgacatt acgttggatg tcagccgccg
     8821 tatatacgaa gccgcccgct aagcttttta cgcctcaaac tttcgttttc gggcatttcg
     8881 tccagactta agttcacaac acctcaccgg agcctgctcc ggtgagttca tataaaggag
     8941 gaacgtatgg ctaatccaac cgttattaag ctacaggatg gcaatgtcat gccccagctg
     9001 ggactgggcg tctggcaagc aagtaatgag gaagtaatca ccgccattca aaaagcgtta
     9061 gaagtgggtt atcgctcgat tgataccgcc gcggcctaca agaacgaaga aggtgtcggc
     9121 aaagccctga aaaatgcctc agtcaacaga gaagaactgt tcatcaccac taagctgtgg
     9181 aacgacgacc acaagcgccc ccgcgaagcc ctgctcgaca gcctgaaaaa actccagctt
     9241 gattatatcg acctctactt aatgcactgg cccgttcccg ctatcgacca ttatgtcgaa
     9301 gcatggaaag gcatgatcga attgcaaaaa gagggattaa tcaaaagcat cggcgtgtgc
     9361 aacttccaga tccatcacct gcaacgcctg attgatgaaa ctggcgtgac gcctgtgata
     9421 aaccagatcg aacttcatcc gctgatgcaa caacgccagc tacacgcctg gaacgcgaca
     9481 cacaaaatcc agaccgaatc ctggagccca ttagcgcaag gagggaaagg cgttttcgat
     9541 cagaaagtca ttcgcgatct ggcagataaa tacggcaaaa ccccggcgca gattgttatc
     9601 cgctggcatc tggatagcgg cctggtggtg atcccgaaat cggtcacacc ttcacgtatt
     9661 gccgaaaact ttgatgtctg ggatttccgt ctcgacaaag acgaactcgg cgaaattgca
     9721 aaactcgatc agggcaagcg tctcggtccc gatcctgacc agttcggcgg ctaacatgca
     9781 aattctcccg gtggcggtaa tgttccgcta ccggactttt cagaaatcat ttattcccct
     9841 cgcgtcccgc ccgttgttac tcttccttgt tcaggaatgc caaatataag gacatcatca
     9901 tgcagagccg gaagctctta aaagaacaac tcatctatat ccgggataaa cgcaacggag
     9961 aggtgaaaaa cagatgaaaa taatacttct gtttttagca gccctggcaa gttttaccgt
    10021 acacgcgcag cctccttcac agaccgtaga acaaacagtc cggcagattt atcagaacta
    10081 taaatcagat gccagtacgc cttatttcgg tgaaaccgga gagcgggcga taacttcagc
    10141 gcgtattcaa caggcgctta ccctgaacga caatcttacg ctgccgggca atattggctg
    10201 gctggattat gatccggttt gtgattgtca ggattttggc gatctggtgc tagaaagcgt
    10261 tgcgataacc caaactgacg ccgatcatgc cgatgccgtt gtgcgctttc gtatctttaa
    10321 agatgataaa gaaaagacca cgcagacact gaaaatggtg gcggaaaatg gtcgttgggt
    10381 cattgacgat attgtcagca atcatggcag tgtcttacaa gcagttaata gcgagaatga
    10441 aaaaacgctg gccgctttag cttcgttgca aaaagaacag ccggaagcct ttgttgccga
    10501 actctttgaa catattgctg attatagctg gccgtggacg tgggtggttt ccgactctta
    10561 ccgccaggcg gttaatgcct tctataaaac caccttcaag acggccaata atcccgatga
    10621 agatatgcaa atagaacggc aatttattta cgacaatccg atctgttttg gcgaagagtc
    10681 gctattttca cgcgttgatg aaattcgagt cctggagaaa accgccgatt ccgcccgcat
    10741 tcatgttcgt tttacgctga ccaatggcaa caacgaagag caagaactgg ttttacagcg
    10801 gcgcgaaggc aagtgggaaa tcgctgattt tatccgcccg aacagcggca gcctacttaa
    10861 gcagattgag gcaaaaactg ccgccagatt aaagcaatga gctgaattaa ataacaatta
    10921 gccggaacaa taaataaaag ggaacactat atgaaaacga ttttcaccgt gggagctgtt
    10981 gttctggcaa cctgcttgct cagtggctgc gtcaatgagc aaaaggtcaa tcagctggcg
    11041 agcaatgtgc aaacattaaa tgccaaaatc gcccggcttg agcaggatat gaaagcacta
    11101 cgcccacaaa tctatgctgc caaatccgaa gctaacagag ccaatacgcg tcttgatgca
    11161 caggactatt ttgattgcct gcgctgcttg cgtatgtacg cagaatgata aaaaaatccc
    11221 cggcagcatg tcagttgccg ggttataggc tgtttgctaa aaaaaagcca aacataagga
    11281 catgattatg cgacgctggc acacctgttt ttttattctg ctgttctgtg gcttcagcgg
    11341 aaacgtgttg tgcaaagaat
//
LOCUS       DAJTYD010000124        11286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTYD010000124 DAJTYD010000000
VERSION     DAJTYD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.124"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            289..1266
                     /gene="csiD"
                     /locus_tag="OO585_002931"
     CDS             289..1266
                     /gene="csiD"
                     /locus_tag="OO585_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6630453.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1286..2554
                     /gene="lhgO"
                     /locus_tag="OO585_002932"
     CDS             1286..2554
                     /gene="lhgO"
                     /locus_tag="OO585_002932"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6630454.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFNDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            2577..4012
                     /gene="gabD"
                     /locus_tag="OO585_002933"
                     /pseudo
     CDS             2577..4012
                     /gene="gabD"
                     /locus_tag="OO585_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4026..5292
                     /gene="gabT"
                     /locus_tag="OO585_002934"
                     /pseudo
     CDS             4026..5292
                     /gene="gabT"
                     /locus_tag="OO585_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5530..6891
                     /gene="gabP"
                     /locus_tag="OO585_002935"
     CDS             5530..6891
                     /gene="gabP"
                     /locus_tag="OO585_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6630455.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPVKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            6950..7612
                     /gene="csiR"
                     /locus_tag="OO585_002936"
     CDS             6950..7612
                     /gene="csiR"
                     /locus_tag="OO585_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6630456.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7613..8062)
                     /gene="kbp"
                     /locus_tag="OO585_002937"
     CDS             complement(7613..8062)
                     /gene="kbp"
                     /locus_tag="OO585_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6630457.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8146..8304)
                     /locus_tag="OO585_002938"
     CDS             complement(8146..8304)
                     /locus_tag="OO585_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6630458.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8487..8786
                     /locus_tag="OO585_002939"
     CDS             8487..8786
                     /locus_tag="OO585_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6630459.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8796..9320
                     /gene="ygaP"
                     /locus_tag="OO585_002940"
     CDS             8796..9320
                     /gene="ygaP"
                     /locus_tag="OO585_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6630460.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9367..9771)
                     /gene="stpA"
                     /locus_tag="OO585_002941"
     CDS             complement(9367..9771)
                     /gene="stpA"
                     /locus_tag="OO585_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6630461.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10439..10888
                     /gene="alaE"
                     /locus_tag="OO585_002942"
     CDS             10439..10888
                     /gene="alaE"
                     /locus_tag="OO585_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6630462.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGIYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10925..11269)
                     /locus_tag="OO585_002943"
     CDS             complement(10925..11269)
                     /locus_tag="OO585_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6630463.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         2663 a         2853 c         2979 g         2791 t
ORIGIN      
        1 gtttactaac ggtcattttg cagtgaagcc atttactgtt ttttatcgac cagataatct
       61 gttctctaat gttaactccc cctaacctgt tgctttagtt attcatttcc tgtctcactt
      121 tgccttaata ccctacgtta aatgttacta atttgttgtt tttgatcaca ataagaaaac
      181 aatatgtcgc ttttgtgcgc atttttcaga aatgtagata tttttagatt atggctacga
      241 aatgagcatc gccatgtcac cctacatctc ataagaggat cgcttctgat gaatgcactg
      301 atcgccgtac aaaataacgc tgtcgattca ggccaggact atagcggatt cactctcatc
      361 ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca ccgaacagac gaccaaacag
      421 tttctcgaac aggttgccga atggcccgtg caggcgctgg agtacaaatc gtttctgcgt
      481 tttcgggtag gcaaaattct tgacgatctg tgtgcgaatc agctgcaacc attgctgttg
      541 aagcccctgc taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg tatcgatgat
      601 gtggcgcagg cagatgagat ggtgaagctg gcaacggcgg tagcgcatct gattggtcgc
      661 tcaaatttcg atgcgatgag cggtcagtat tacgcgcgtt tcgtggtgaa aaatgtcgat
      721 aactcagaca gctatctgcg tcagccgcac cgcgtgatgg aactgcacaa cgacggcacg
      781 tatgtcgaag agatcaccga ttacgtgctg atgatgaaaa tcgatgagca aaacatgcag
      841 ggtggaaatt cgttgctgct gcatctcgat gactgggaac atctggacca ctatttccgc
      901 catccactgg cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa
      961 gatgttttcc atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc
     1021 gaccagttcg tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat
     1081 gccattgaaa ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg
     1141 ataaacaacc tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc
     1201 cgtgaactga tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat
     1261 cagtaagcgc aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat
     1321 cggcatgtcg accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct
     1381 ggaaaaagag tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc
     1441 cggggtctat tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg gaaaccgcgc
     1501 caccaaagcc ttttgcgatc aaaacgacat tcgctatgac aactgcggca agatgctgat
     1561 cgccacgtcc gatctcgaaa tggaacggat gcgcgcgttg tgggaacgca cggcggcgaa
     1621 cggtatcgag cgcgagtggt taaacgccga ggaactgcgc gagcgcgaac cgaatatcac
     1681 cgggctcggc ggcatttttg tgccgtccag cggcattgtc agctatcgcg aagtcacagc
     1741 ggcaatggca aaaatcttcc aggccagagg cggtgagatt atttataacg ccgaagtcag
     1801 cgccctcagt gagcataaaa acggcgtggt cgtccgaacc cgtcagggcg gcgaatatga
     1861 agcatcgacg ctgattagct gttccgggct gatggctaac cggctggtga aaatgctcgg
     1921 cctcgaaccg ggttttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga
     1981 gcataaccag attgttaacc atctgattta ccccatccct gacccggcaa tgccgttttt
     2041 gggcgtgcat ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct
     2101 ggcttttaaa cgcgaaggct atcgcaagcg cgacttctca tttaacgaca cgctggaaat
     2161 tttaggctcg tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga
     2221 gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg
     2281 gctttcgtta agcgatctcc agccgtggcc cgccggtgtg cgggcgcagg cggtatcgcc
     2341 ggacggcaag ctgattgacg attttctgtt tgtcaccacc ccgcgcacga tccacacctg
     2401 caatgcgccc tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa
     2461 ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cgacacgtag
     2521 tgtggatgcc ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga
     2581 aacttaacga cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg
     2641 ccaacaatgg cgggatcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg
     2701 tgccgaaaat gggcgctgat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc
     2761 ccgccaggcg tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac tggttcaatt
     2821 tgatgatgga gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggcaaaccac
     2881 tggccgaagc gaaaggcgaa atcagctacg ctgcctcttt tattgagtgg tttgccgaag
     2941 aaggcaaacg tatttatggc gataccattc ccggtcatca ggccgataag cgcctgattg
     3001 ttatcaagca gccgattggc gttaccgcag ctatcacgcc gtggaacttc ccggcggcga
     3061 tgattacccg taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg
     3121 ccagtcagac accgttctct gcgctggcgc tggcggagct ggcgattcgc gcgggcattg
     3181 cggctggcgt gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca
     3241 gcaacccgct ggtacgcaaa ctgtcgttta ccggttcgac cgaaattggc cgacagttaa
     3301 tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt
     3361 ttatcgtctt tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat
     3421 tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt ggcgtgtatg accgctttgc
     3481 cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc gacgggctgg ataaaggcgt
     3541 caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa gtggaagagc atattgccga
     3601 tgcgctggag aaaggtgcgc gtgtggtttg cggcgctaaa gcggatgaac gcggcggcaa
     3661 cttcttccag ccgaccattc tggtggacgt gccggccaac gccaaagtgt cgaaagaaga
     3721 gacgttcggc cccctcgccc cgctgttccg ctttaaagat gaagccgatg tgattgcgca
     3781 agccaatgac accgagtttg gccttgccgc ctatttctac gcccgtgatt taagccgcgt
     3841 cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc atcaataccg gcattatttc
     3901 caatgaagtg gccccgttcg gcggcatcaa agcctcgggt ctgggtcgtg aaggttcgaa
     3961 gtatggcatc gaagattact tagaaatcaa atatatgtgc atcggtcttt aactggagaa
     4021 tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag tcaggcgatt ccccgtggcg
     4081 ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg
     4141 aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc gggcacctgc
     4201 atccgaaggt ggtggccgcg gtggaagcgc agttgaaaaa actgtcgcac acctgcttcc
     4261 aagtgctggc ttacgagccg tatctggagc tgtgcgagat tatgaatcag aaggtgccgg
     4321 gcgatttcgc caagaaaacg ctgctggtta cgaccggttc cgaagcggtg gaaaacgcgg
     4381 taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat cgcttttagc ggcgcgtatc
     4441 acgggcgcac gcattacacg ctggcgctga ccggcaaggt gaatccgtac tctgcgggca
     4501 tggggctgat gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg cacggcataa
     4561 gcgaggatga cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc gcgccggaag
     4621 atatcgccgc catcgtgatt gagccggttc agggcgaagg cggtttctac gccgcgtcgc
     4681 cagcctttat gcagcgttta cgcgctctgt gtgacgagca cgggatcatg ctgattgccg
     4741 atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt tgcggtggag cagatgggcg
     4801 tggcgccgga tcttaccacc tttgcgaaat cgatcgctgg cgggcgtcac cgggcgcgcg
     4861 gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg taacccgatt
     4921 gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct gctgcaaaaa
     4981 gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga aaaacacccg
     5041 gagatcggcg acgtacgcgg gctgggagcg atgattgcca tcgagctgtt tgaagatggc
     5101 gatcacagca aaccggacgc aaaactcacc gccgggatcg tggcacgcgc ccgcgataag
     5161 ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct tgtactgctc
     5221 accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg ttttgatgag
     5281 gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt ccggcctacg
     5341 agaatccagg tcggataagg cgtttacgcc gcatccgaca atctgtacgt gaacaggaat
     5401 aaatctatgt cggccggata atgcggagcc gctctccggc aaaaagaatc aataacaatt
     5461 aaacgcgtga cccggcgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga
     5521 ggattcagga tggggcaatc atcgcaacca catgagttag gcggcgggct gaaatcacgc
     5581 cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc
     5641 gtcgccatcg ccgaagcggg cccggcagta ttactggcct atctgttcgc cgggctactg
     5701 gtggttatga ttatgcggat gttggcggaa atggcggttg ccacgcccga taccggttcg
     5761 ttttccacct atgccgataa agccattggt cgctgggcgg gctataccat cggctggttg
     5821 tactggtggt tttgggtact ggttatcccg ctggaagcca acatcgccgc catgatcctg
     5881 cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac ccttgcctta
     5941 actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg
     6001 tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt
     6061 tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc
     6121 aacggcttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca
     6181 gaaattgtca ccattgccgc cgcggaatcc gacacgccgg aaaaacatat tgtccgcgcc
     6241 actaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtcgtg
     6301 gcattaattc cgtggaatat gcccggacta aaagccgtcg gttcttatcg ctcggtactg
     6361 gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt actttccgta
     6421 accagctgcc tgaactcggc gctgtatacc gcttccagga tgctctactc cttaagccgt
     6481 cgcggagatg cgcctgcggt aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg
     6541 gtgttactct ccaccggcgc ggcattctta acggtggtgg tgaactatta cgtgcctgtg
     6601 aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt ttatttagtc
     6661 atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc
     6721 ttgcgcatgt ggctttatcc ctggctcacc tggctggtca tcggctttat tacctttgtg
     6781 ttggtagtga tgctattccg cccggcgcaa cagttagaag tgatctccac cgacttatta
     6841 gcaataggga ttatctgtac cgtgccaatt atggcggctg gaataagctg ataatgtggc
     6901 aaaaaacacc cgttcataat acgcgctgat aataatcagg agtcacacca tgaccattac
     6961 gtctctggat ggctatcgct ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga
     7021 tgaaaaatta cggatgagtt tgctgacatc gcgttatgca cttggcgttg ggccgttgcg
     7081 ggaagcactt tctcaactgg tggcggaacg gctggtcacg gtggtgaatc aaaaagggta
     7141 tcgggtggcg tctatgtcag agcaggagct gctcgatatt ttcgatgccc gcgccaatat
     7201 ggaagcgatg ttagtgagtc tggcgattgc ccgcggtagc gatgagtggg aggcagacgt
     7261 tctcgcaaaa gcgcatctgc tcagtaagct ggaagcctgt gacgccagcg agaaaatgct
     7321 tgatgagtgg gatctgcgtc atcaggcgtt tcatacagca attgtggcgg gctgtggttc
     7381 tcactatttg ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgct atcgatttat
     7441 ctggctgcgg cgaacggtgc tttcggtaga aatgctggag gataaacacg atcagcacca
     7501 gaccctgact gcggcggtac tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca
     7561 gcatttactg acgccaattc ccattatcca gcaggcgatg gctggcaatt aactactctt
     7621 ccggaatacg caacacttgc cccggataaa ttttatccgg gctttttagc atcggtttat
     7681 tcgcttcgaa gattttattg tacagattag cgttaccgta gacctgtttg gaaatggcac
     7741 tcagagtgtc gccagactta acggtataaa actggctggc agtggctggt gtcgccgttt
     7801 tcacctgatc atcaacactg gcaataccgg aaatattccc caccgcaaca aggattttct
     7861 ccttcgcctc ctgactcagg ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa
     7921 tgttcacttt atcggcatca ggtataccgg ttttgctcag atgctcctgc accttcttcg
     7981 cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca gagtttttct ccggcatctt
     8041 tcacaaaatt gaacagaccc atagctacct cattcgttaa cggaaacact cagaaagtgt
     8101 agcagaggaa cgcggcgcgt taacgccgcg ggggggagca caggtttaat cgcgggtttg
     8161 cacccagaac gcgtgaatca aaccggggat atacccaagc agtgtcagca gaatattaat
     8221 aatgaacgcc caaccgaacc ctttaccgag cagcacgccg agcggcggca gaatgatggt
     8281 gatgacgatt ctccagaaac ccatatgtac tccctataag aaaattactc attgtttaaa
     8341 aagagatttt atctcttaag cgtagtaatt ttagcggagc ctgccagttt cagcttacgc
     8401 ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt tagagtttac
     8461 ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag gccagtgccg
     8521 aacaggcagc ggccttattg aaagcaatga gccatcctaa acgattgctg attctgtgca
     8581 tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc ggactgagtg
     8641 cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc gacagccaac
     8701 gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc attatcgcca
     8761 ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc atttcgccgc
     8821 atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt gacgctgatg
     8881 aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg ctggaacagt
     8941 caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag gcaggtaaac
     9001 gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa atatttttac
     9061 tcgaagatag gattgatggc tggaaaagag ctggattgcc agtagcggta aataaatctc
     9121 aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata ttaatcggcg
     9181 ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt gttggtgccg
     9241 ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta gataagatgc
     9301 cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga catccggcct
     9361 cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct gagcaattgg
     9421 cttcggtgta cggccctgac cggtccaggt cttagtttca ccgttaacat cagtgaattt
     9481 atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag cagagctatt
     9541 acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc aggtgctaat
     9601 tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac gtctttcttt
     9661 agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga attcgcgagc
     9721 catcgcacgg agggttcgaa tgttatttaa actttgtaac attacggaca taataaagaa
     9781 aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta
     9841 tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag ctgttatttt
     9901 ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag taatttaggt
     9961 tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgat aaaaccgaag
    10021 tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg ctttctgctg
    10081 ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcacga caaacaccga
    10141 attctctacg cttatagcgt tacctcaccc ccaaactttc attagtgaaa ttaaaggcaa
    10201 ggtcatatcc aattgattta attgaaaata aatcatatcg gcatgtaaaa atccacaatg
    10261 tacaaaaaac gagctgttac ggaatatttt atctacaaaa aactgactaa ataaaagttt
    10321 ttcactaatt gattagtcat agccagcgat atacgctatg cgaaaatgca gatggcaatg
    10381 agatccactg ctttcatctc cattaacatc ccattacgct ttattaagga gcattagcat
    10441 gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga tggttgttta
    10501 ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct tcgaacagtc
    10561 tttttattcc agattggtag cgattccggt gaacatctta attgcatggc catacggtat
    10621 ataccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct ggataaaaaa
    10681 tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg cgatcttgtt
    10741 agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca tcgttgtttc
    10801 gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac tgtttaaagt
    10861 cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat cgccagtcgc
    10921 gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca acacgcgaac
    10981 tgaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag agcggaaatt
    11041 gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt gaactgcgtt
    11101 cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg cgattaacat
    11161 agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca tcgacagtaa
    11221 aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata tttgatcctc
    11281 gttatt
//
LOCUS       DAJTYD010000125        11275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTYD010000125 DAJTYD010000000
VERSION     DAJTYD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.125"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            52..207
                     /locus_tag="OO585_002944"
     CDS             52..207
                     /locus_tag="OO585_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630464.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            407..691
                     /locus_tag="OO585_002945"
                     /pseudo
     CDS             407..691
                     /locus_tag="OO585_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
     gene            complement(729..2462)
                     /gene="ggt"
                     /locus_tag="OO585_002946"
     CDS             complement(729..2462)
                     /gene="ggt"
                     /locus_tag="OO585_002946"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6630465.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            2582..3022
                     /locus_tag="OO585_002947"
     CDS             2582..3022
                     /locus_tag="OO585_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6630466.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(3009..3752)
                     /gene="ugpQ"
                     /locus_tag="OO585_002948"
     CDS             complement(3009..3752)
                     /gene="ugpQ"
                     /locus_tag="OO585_002948"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6630467.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(3749..4819)
                     /gene="ugpC"
                     /locus_tag="OO585_002949"
     CDS             complement(3749..4819)
                     /gene="ugpC"
                     /locus_tag="OO585_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6630468.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(4821..5666)
                     /gene="ugpE"
                     /locus_tag="OO585_002950"
     CDS             complement(4821..5666)
                     /gene="ugpE"
                     /locus_tag="OO585_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6630469.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(5663..5866)
                     /locus_tag="OO585_002951"
                     /pseudo
     CDS             complement(5663..5866)
                     /locus_tag="OO585_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            complement(5796..6467)
                     /locus_tag="OO585_002952"
     CDS             complement(5796..6467)
                     /locus_tag="OO585_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR6630470.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            complement(6864..7577)
                     /gene="livF"
                     /locus_tag="OO585_002953"
     CDS             complement(6864..7577)
                     /gene="livF"
                     /locus_tag="OO585_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6630471.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(7579..8346)
                     /gene="livG"
                     /locus_tag="OO585_002954"
     CDS             complement(7579..8346)
                     /gene="livG"
                     /locus_tag="OO585_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6630472.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(8343..9620)
                     /gene="livM"
                     /locus_tag="OO585_002955"
     CDS             complement(8343..9620)
                     /gene="livM"
                     /locus_tag="OO585_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6630473.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(9617..10543)
                     /gene="livH"
                     /locus_tag="OO585_002956"
     CDS             complement(9617..10543)
                     /gene="livH"
                     /locus_tag="OO585_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6630474.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(10591..>11275)
                     /gene="livK"
                     /locus_tag="OO585_002957"
     CDS             complement(10591..>11275)
                     /gene="livK"
                     /locus_tag="OO585_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6630475.1"
                     /translation="LDSSQGPTAAKYILETVKPQRIAIIHDKQQYGEGLARSVQDGLK
                     AANANVVFFDGITAGEKDFSALIARLKKENIDFVYYGGYYPEMGQMLRQARSVGLKTQ
                     FMGPEGVGNASLSNIAGDAAEGMLVTMPKRYDQDPANQGIVDALKADKKDPSGPYVWI
                     TYAAVQSLATALERTGSDEPLALVKDLKANGANTVIGPLNWDEKGDLKGFDFGVFQWH
                     ADGSSTAAK"
BASE COUNT         2719 a         3242 c         2813 g         2501 t
ORIGIN      
        1 ttagagatta ccagcagaaa attccttgtt cgaaatttag cagtgcaagc tatggaaaaa
       61 cttacaacgg aactacactc cttaagtgag atggaccgaa gacatgtagt ctctatttta
      121 gccgaaatag ctaatggcta tgatgatttt aatgacatgt tgatttattt agaattttac
      181 ccgaatcata agattctgat atcttaaata agaaaatccc gttcgacgtt ctgaaaaaga
      241 aatcataaaa tattagcaga tacttatttc gtgactatat cttatccgat gaactttttt
      301 caaaaccagc atttaataat atctttgacg aagatgaacg cgtctttgct caatgtatca
      361 cacaggaata ataatataac actcaacaat aatatttatt ataattatga ttacttatca
      421 cgacgcattc gcgaaagcga accattacct tgatgatgca gatctcccgg tcgtcattac
      481 tctacatgga cgctttagct agggctggta tttctgtttc gaagcacgag aatttctcga
      541 aactggagat gaggccgcgc gcttagctgg taacgcacct tttattattg ataaagacag
      601 tgctgaaatt cattctctgg gaacggcaaa gccgctggaa gaatatctac aggattacga
      661 aataaaaaag gctaccttcg gcttgccctg acaaaatagc cctcttccca cgaagagggc
      721 cgctaacctt agtaccccgc cgttaaatca tccaccgagc gcgggtcgga tgcgccgtac
      781 aactcaccgt ccggcccaac cataatgctt tgtgtactgc ccatcgcctc tttcagcgcc
      841 actttctgac cttttgcttc cagcagcttg agcgtatccg ggctaaaccc tttttcgaca
      901 cgcagctcgt ccggcaacca ctgatggtgg aaacgcggcg cattggtcgc ttcggcgacg
      961 ttcatgccat aatcgatgct attcaccacc atttgcagca ctgtagtgat gatccggctc
     1021 ccgccagggc taccggtgac cagccaggtt ttaccgtctt tcaccacaat cgttggtgac
     1081 attgacgaca gcggacgttt attcggcccg acggcgttgg catcaccgcc caccagcccg
     1141 taaacgttcg gtacgcccgg tttagcggag aaatcatcca tctgattatt aagcagaata
     1201 ccgctctcgc ccgcgacaat gcccgtaccg aaggtggtgt tcagcgtata ggtcaccgcc
     1261 accgcgttac catctttatc caccactgag taatgggtag tttgattact ctcataaggc
     1321 gcaagcttgc cggggcgaat ttcgctggat ggctgcgctt tattgatatc aatttgatcg
     1381 gcaatagatt tggcataggc tttattggtc agcgcctgcc acggtacttt gacaaaatcc
     1441 gggtcgccaa gatattccga gcggtcggcg taggcgtatt tctccgcttc tgccatgatt
     1501 tgcatcgcat cggcgctgcc aaagccgtat ttcttcatat cgaagttttc cagaatattg
     1561 aggatttgta cgatatggat cccgccggag gatggcggtg gcatggagta aacctgatac
     1621 ccgcgataat cgccacttat cggtgtgcgt tcgaccgctt tatacgccgc taaatcttct
     1681 ttagtgatca agccaccgtt tttctgcatc tcctgggcaa tctgttccgc tatcgtgcct
     1741 ttatagaatt cgtccgggcc gttttcggca atcatctcca ggctctttgc caggttcgcc
     1801 tgcaccagct tgtcgccctt tttcagcggt tcgccctctt tccagaagat agctttgctg
     1861 ttttcgtgat tcggcaacac ttcgctaccg taggttttga gatcgtctgc caaagcatcg
     1921 ttaacgataa aaccatcgcg tgccagtttg aacgcgggct gcaccacttt gttcaacggc
     1981 atagtgccgt acttatccaa cgccagcgag aaaccagcga ccgtaccggg cgtgccggaa
     2041 gccagatgcg aagtgagtga ttttttgctg tccgggttgc cctggtcatc aaggaacata
     2101 tcgcgggtcg ctttagcggg tgccatctcg cggaaatcga tagcggtggt attgccattt
     2161 ttcgagcgga tcagcataaa accaccaccg cccagattcc ctgcctgcgg atgcgttacc
     2221 gccagcgcgt agcccaccgc cacggcggca tcaacggcat tcccgccctc ctttagaata
     2281 tctaccccca cttgggtggc agtggcatcg acagacgcca ccatcccctg tttcgcgcgt
     2341 accggatgga agatatcttc ctccacgccg taagaaacag gaggcgcggc gacggcgcta
     2401 aaacaacttc ctgagagcag agcagcaatg gctacccggc gtaaaaacgt cggttttatc
     2461 atcgttattc tccagagatt aaggggcaac cccagctaag cctggtatat aactctgaat
     2521 taatcatcgt tttgtcggga agcgagtaaa cttaaaggat atcttcagag gaggatacgc
     2581 gatgaaacga cttctgattc ttacggcact cctgccgttt gtcggctttg cacagcccat
     2641 taatactctg aacaacccta accagccggg gtatcagata cccagccagc agcggatgca
     2701 aacccagatg caaactcagc aaatccagca aaaagggatg ctgaatcagc aactgaaaac
     2761 gcaaactcag ttgcaacagc agcatttaga aaaccagata aacaataatt ctcagcgggt
     2821 gttgcagtca cagccggggg agcgaaatcc cgcccggcag caaatgctgc ccaacaccaa
     2881 cggcgggatg ttaaacagca accgtaatcc ggatagttcg ttgaatcagc agcatatgct
     2941 gccggagagg agaaacggcg acatgctgaa tcagcccagc acgccgcagc ctgacattcc
     3001 gttgaaaact attgggccgt aaagttcgga ccaatcacgt caatcgcatc ggtacagatg
     3061 caatccacgc cccagcgcag caactctgcc gcgcgctggg gtttattgac ggtataaacc
     3121 agaatccgta atccggcgtc tttcaactgc atcactcgcg ctttatcgag taacttatga
     3181 ttgagatgaa tagagacgca gcccagccgc gcggtcagtt cgcgccagtc gtcgcgccac
     3241 tcatcaagca ataaaccacg cggcagttcc ggtgccgcca gctgtgcagc ttctaaagca
     3301 tcaatctcaa acgatgacag cagcggcggc gtcataccgg cccacagttg gcgtgccgcc
     3361 agcgccacca ttttgcccgt taatggtcca gtgccagtgg tgggtttgat ttcgatattc
     3421 gccatcatcc catgttcgcg acagcgttcc gccacctgcg aaagcaacgg cagcggctca
     3481 cctttaaacg ctttgctgta ccaactgccc gcatccacgc gcagtaaatc ctgccagttc
     3541 agttcaccca cgacgcccca gccgttgctg gtgcgttcga gattgtcgtc atggagcagg
     3601 aagatctcgc catctttcga taacttcgcg tcaaattcga tcatcttatg accgtatttt
     3661 gccccgacgt cgattgccgc cagggtgttt tccggggcca gcttaccgcc gccacgatga
     3721 gcgacgatgc ggggataagg ccagttactc atactcgttg tcctgtttca ccatcaaaaa
     3781 gatgcagctg attttccgcc agatgcagcc acagcgtgct gcctgccgtc gggcgctcct
     3841 gatgcgccag tcgcaccacc agcttctgct cgccccagcg tccgtgcgcc aggttatctg
     3901 cgccgaggat ctccagcgtg tccatcacca gcggtacgcc gccttctgcc tgcgagctta
     3961 gcacaatatg ttccgggcga atgccgagag tcattttacg cccggcatac tgacggtagc
     4021 caccgtttag cggcagctca ataccgccgt ccagttcgaa atgcgtgcct tcgttattca
     4081 cgcggcctgt cagcaggttc atcgccggac tgccgataaa actcgctaca aacaggctgg
     4141 cgggcttttc gtagacttca actggtgtgc caatctgttc ggcaacgccg ccgttcatca
     4201 ccattactcg ctgggcgagc gtcatcgctt caacctgatc gtgagtaacg tagagtgaag
     4261 tcgttttcag gcgacggtgc aactgttgca gttcaagacg catctgcacg cgcagcttgg
     4321 catcgaggtt agagagcggc tcatcaaaca ggaacaccgc cggatcgcgc acaatcgcgc
     4381 ggcccatcgc tacacgctga cgctgaccgc cggatagctc acgcgggcga cgtttgagca
     4441 gaccgtccag ctccagaatg cgcgccgctt ctttaacgcg ctcggcaatt tgctgcttgc
     4501 ccatgccgcg aattttaagc ccccacgcca tgttttcttc gacgctcata tgcggataaa
     4561 gcgcgtagtt ctggaacacc atcgcaatcc cgcgatcttt cggctccatt tcagtcacgc
     4621 gctggtcgtt gatccagata tcgccttcgg tcacccgctc cagcccggca accatacgca
     4681 gcagcgtcga tttcccgcag ccagacggcc cgaccatcac gataaattcg ccatcagcca
     4741 catcaagggt cagcggttta atgacctggg ttttgccatc ccagcttttg gttactgcct
     4801 gtaatttcag tcctgccatc ttatttctca ctatcgacca ggccgcgcac gaaggcacgc
     4861 tgcatcacta aaacaatcac tactggtggg atcagggtta acaacatcgc cgccatcact
     4921 gagttccatt ccgtggtgcc ttcgccagtg gcgatcatcc ctttgatccc tgccacggtg
     4981 gtgccgagat ccacgtcggt aataatcaac aacggccaca aatactggtt ccagccgtaa
     5041 ataaaggtga tcacaaacag cgccgccaga ttagttttgg agagcggaaa aacgatgtcg
     5101 caaaagaagc gcattggcga tgcgccgtcg atccgcgccg cttccaccag ttcatccggc
     5161 agcgtcataa agaactggcg aaacagaaac gtggtggtcg ccgaggccat cagcggcagc
     5221 gttaaaccgg cgtagctgtc gagcatcttc aggttggcga tgacttccac cgtcgggaag
     5281 atacgtactt caaccggcag catcagggtg ataaaaatca tccagaagaa gaggttacgt
     5341 agcggaaaac gaaaccagac aatggcaaat gccgagagca tcgagacggt aattttgccg
     5401 agcgtaatgc tgaacgccat cacaaagctg ttaagcaaca tccgccagaa cggcgcgcta
     5461 ttcgtaccta ccccgttaac ccagatgttg tggatatttt ccagcagatg tgtgccgggg
     5521 atgagcgtca tcggcgcggc atagacggcc tgtttatcca gcgtcgccgc gacaaacgcc
     5581 acgtacagcg ggaagagaat caccgcgatc ccgaggatca gcatggtatg gctgaatatc
     5641 gtcagccacg gacggttctc aatcattggt aacgcacctt gctttcaaca tagcggaact
     5701 gcaccaccgt cagcacgatg acgaggaaca tcaacaccac cgactgtgcg gcagacgaag
     5761 ccagatccag tccggtaaaa ccttcgcggt agatcttata aatcagcgtc gtggtggcct
     5821 gaaccggccc gccggacgtg gcggcgtcga tcaccgggaa ggtgtcgatt ttccagttgg
     5881 atccagccct gccagccgct ggcgtagccg cacttcatgc cggaggcttt cagtttcgcg
     5941 gcatagtccg ccagatcctg ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc
     6001 ttgaaggcgt ctttgttgta atagagaacg ggagtcgagc tgttgaatgg ctgggagagt
     6061 aagtggcccg ttttgctgtc ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca
     6121 tcgaattgaa tccccgcctc tttaaacacg tcatataccg gtttaatggc tttcgatgcc
     6181 atcatggtgg cggtgccaac ttcataaacc tgcaaaatag ccggagcgtt gccggtacga
     6241 aatgcggcaa tccccgcgct taaattctgt tcgtagttgc ctttataggt cggtacaatt
     6301 ttgtaatccg gattttcggc gttaaaacgt tgggccagag aatccacctc tttacccagt
     6361 tccccttcca tagaatgcca gaacggaatg gtcgtcactg cctgtgcatt ccccattaac
     6421 gccagtccga gcgccagtgc tgaagctgta taatgtaacg gtttcatcgt ttatctctct
     6481 tgttgtaccg aatgcgcgaa ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg
     6541 aattacagaa aaataacttt tttgttacat ttgtaagata gtaaggtgtc agaaagatga
     6601 caaggcggtg acggcgtggg tgagggaaaa tgggagatgg ggcacggata agcggaaaaa
     6661 tagaaggtct gaatcaaact ctacagatta ctcatcgttt catgccggat gcggcgtaaa
     6721 cgccttatcc ggcctacaag atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc
     6781 tgataagcgc agcgcatcag gcaattttac atttgtcacc tgtctcaaag gagtcttttg
     6841 actccttatc aatcaacgtg ttattacccg cctaaatacg cacttctcac tgcttcgttc
     6901 gccagcagcg catcgccggt atcggagagc accacatggc cgttttccag cacgtagccg
     6961 cgatccgcca gctttagcgc ctggttggcg ttctgctcga cgaggaagat agtcatcccc
     7021 tgctcgcgca gctgctcgat ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg
     7081 agcgatggct catcaagtag cagcaaacgc gggttgctca tcagcgcgcg accaatcgcc
     7141 agcatttgtt gctcaccgcc ggacatggtg cctgcccgct gaacacggcg ctcatgcaga
     7201 cgtggaaaca gttcatacac ccactttatg cgctcctgga actggtcgcg ttcggcaaaa
     7261 aagccgccca tcgccaggtt ctcttccacc gtcatccgcg agaagacgcg acgcccttcc
     7321 gggacaatcg ctaccgcttc gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta
     7381 tcatcaaaca caattcgccc gctggcggcg cgcggatcgc cgcataacgt gccgagcaag
     7441 gtggttttcc ccgccccgtt cgcgccaatc agcgtgacaa tctcgccctg attgatatgc
     7501 aggctcacct catgcagtgc ctggattttg ccgtagtggg cgctgacttt gtcaaaggac
     7561 aacatgactt tttccatctt atgcctcacc taaataggca cggatcacgt ccgggttatt
     7621 acggatttgc tccggcgtac cgtttgccag tggcgtcccc tgattgacca cgtaaattcg
     7681 gtccgaaatt cccatcacca gcttcatatc gtgttcaatc aacaagatag tggtgttgtg
     7741 atgattgcgc agttcggcaa tcagctcatc cagctctttc gtctctttcg ggttaagacc
     7801 tgccgcaggt tcgtcgagca ttaaaatctc cggctgcgtc accatgcagc gggcaatctc
     7861 aagacggcgc tggtcaccat aggccaggtt actcgcctga cggttggcgt gttccagcaa
     7921 accaatgcgc tcaagccagg tcgcggcgcg atcgagcgct tcgctctggg cgcgacggaa
     7981 ggatggcgtt ttcaacaggc cagagaacag cccggttttc agttgctgat gctgcgccac
     8041 cagcaggttt tcaatcaccg tcatttcacg gaacagacgc acatgctgga aggtacgcac
     8101 cacgcccatg cgggcaattt gctgccccgg taaaccttcc agatgctgat cgcgcagtaa
     8161 aatggtgcct ccagtgggtt tgtagaatcc ggtcaggcag ttaaagaccg tggtttttcc
     8221 ggcaccgtta ggaccgatta acgagacgat ctcctgcggg tacagttcaa gattgacgtt
     8281 gttcaccgcc agcaggccgc cgaagcgcat catcaggccg ttcacagata ataatggctg
     8341 actcatgcct gctctccttt cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg
     8401 ggcagcaagc cctgcggacg ccagatcatc atcagcacca tcaaaccacc gagcattaac
     8461 atgctgtatt cgttgaaatc acgcatcaac tcgcgcgaca ccaccagcaa aattgccgcc
     8521 agaatcaccg caaactgcga tcccataccg ccgagcacca ctatcgccag tacaaacgcc
     8581 gattcagcaa aggtgaagga ttccgggctg acaaagccct ggcgcgcagc aaacagcgtt
     8641 ccggcaaaac cggcaaacgc ggcactgatg gtaaaagcgg tcagcttgat acgacgcggg
     8701 cttaagccca gcgaacggca ggcgatctca tcttcacgca acgcttccca cgcacgcccc
     8761 agcggcatcc gcagcaggcg gttaatgaca aacaggctta gcaccaccag cagcaacgcc
     8821 accagataca ggaagatgac gcgatcggag ggatcgtatt tcaggccaaa gaaattactg
     8881 aacgtgtccc aaccgccttc acgggcggta cggctgaact caaggccgaa gaaagtcggt
     8941 ttcgggatct gactgattcc gttcgggccg ccggtaattt cggtgttatt gagcagcaat
     9001 atgcgcacaa tttcgccgaa gccgagggta acgatcgcca gataatcacc gcgcagacgc
     9061 agcaccggaa agccgagcag gaagcccgcc gccgctgcca ttaacccggc aatcggcaga
     9121 caagtccaga agcccaagcc gtaatagtga ttgagcagcg caaaggtgta agcgccgatg
     9181 gcgtaaaaac cgccgtaccc cagcaccagc agaccagaaa gaccaacaac cacattcagc
     9241 ccgagaccga ggataatgta gatcatggtc agagtggcga tatccaccgt cccacgtgac
     9301 accataaacg gccacgccac cgcaagcacc aacagcgcca cgaggaacag tttctgcttc
     9361 accgtggagc catcaatcgc gggcagaata aacttcggtc cggaaacgct ttttagccct
     9421 ttctggaaag ccggtcgcaa aagctggaag aaaaagacca ccgccgtgcc gataaacacc
     9481 cactgccaac ggatgtctga agccgtgtcg accaccagtt tggtgccatc cagctccagt
     9541 tgcacgccca taaagacgcc cgccaggaca aaaaacatcg cggcagagag cagcgccatt
     9601 gcaatatgca tcggtttcat actttctcta cctccgggcg acccagaata ccggtcggca
     9661 tcaccagcag caccagaatc agcagggcaa atgaaaccac atccttatat tccgtactca
     9721 gataggcaga agagagcgcc tccgcaatcc ccaggatcag cccaccaatc atcgcccctg
     9781 gaatgctgcc aatcccaccg agcaccgcag cggtaaaggc tttcatcccg gccataaagc
     9841 cgatataggg gttaatgacg ccgtagaact gaccgagcag tacgccagcc actgccgcca
     9901 tcgccgcgcc aataacaaag gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc
     9961 tcgccatttt cagatcttcc gcgcaggcac ggcaggcgcg ccccatacgg gaataacgaa
    10021 tgaaaatcgt cagcgccagc atggcgagga aagtaacaat ccagatcact gcctgcatgg
    10081 tggtaataga ggcagagaag ttttcgctat gccccaccac ccactgaccg ttaaacagac
    10141 tgggcagcgc cacgtcacgc gaaccttcag tcaggctaac gtagttttgc aggaagatgg
    10201 acatgccgat tgccgagatg agcgcaatca ggcgcttaga gttacgcacc gggcgatagg
    10261 ctacccgttc gatactccag ccgtaggcgc tggcaatgac gattgcgccg acgaatcccg
    10321 ccgccaccag cagccagccg gtatcaatgc ccatcatcat cagcgcggcg atgatcataa
    10381 atgagacgta gctgccaatc atataaacct cgccgtgggc gaagttgatc atgccgataa
    10441 tgccgtaaac catggtgtag ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc
    10501 cgttaaacat ctgctgcaag aaatacaaaa actgctcaga cataaggtaa cctttctaaa
    10561 cccgcccgca ttttacgggc ggtgggatga tcacttggct gccgtggatg aaccgtcggc
    10621 gtgccactgg aagacaccaa aatcaaatcc cttaagatcg cctttttcat cccagttcag
    10681 cggcccaatc acggtgtttg caccgttagc ttttaaatct ttcaccagcg ccagcggctc
    10741 atcgctgcca gtacgctcaa gggcagtcgc cagagattgc accgccgcgt aggtgatcca
    10801 gacataaggc ccggacggat ctttcttgtc tgctttcagc gcatcaacga tgccctggtt
    10861 tgccggatcc tggtcatagc gttttggcat agtgaccaac atgccttcgg cagcatcacc
    10921 ggcaatattc gacaatgatg cgttacccac accttccggc cccataaact gagttttcag
    10981 gccaacggaa cgggcctggc gcagcatctg ccccatttcc gggtagtaac cgccgtagta
    11041 aacgaagtcg atgttttctt ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc
    11101 ggcggtaata ccgtcgaaga agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac
    11161 cgaacgcgcc agcccttcgc catactgttg tttgtcgtga atgatggcga tgcgctgggg
    11221 cttcaccgtc tcaagaatgt attttgccgc cgttggcccc tgggaagagt ccagc
//
LOCUS       DAJTYD010000126        11228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTYD010000126 DAJTYD010000000
VERSION     DAJTYD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.126"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(37..273)
                     /locus_tag="OO585_002958"
     CDS             complement(37..273)
                     /locus_tag="OO585_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6630476.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="OO585_002959"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="OO585_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6630477.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="OO585_002960"
                     /pseudo
     CDS             complement(1240..1916)
                     /gene="bglJ"
                     /locus_tag="OO585_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(1874..2599)
                     /locus_tag="OO585_002961"
     CDS             complement(1874..2599)
                     /locus_tag="OO585_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6630478.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3218..3988
                     /locus_tag="OO585_002962"
     CDS             3218..3988
                     /locus_tag="OO585_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6630479.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     HHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3979..4452
                     /locus_tag="OO585_002963"
     CDS             3979..4452
                     /locus_tag="OO585_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6630480.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="OO585_002964"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="OO585_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6630481.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="OO585_002965"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="OO585_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6630482.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /locus_tag="OO585_002966"
     CDS             5888..6382
                     /locus_tag="OO585_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6630483.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="OO585_002967"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="OO585_002967"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6630484.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="OO585_002968"
     CDS             complement(9066..10088)
                     /gene="lgoD"
                     /locus_tag="OO585_002968"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6630485.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10227..11141
                     /locus_tag="OO585_002969"
     CDS             10227..11141
                     /locus_tag="OO585_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6630486.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
BASE COUNT         2920 a         2880 c         2625 g         2803 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aactaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca cccctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaatg
     1741 accgccgcaa atgagatccg cgacatggca tcctgaaatg actcctgact ggtataggta
     1801 tggagcttat aagggttacc cgcaaacttt ctgaataaac tctcaatacc gatactgctc
     1861 atgacgcatt tttctatgag tgcgacattt ctcttcttaa ttcggctgtg ttccattctg
     1921 cggcatctcc ataaacagaa ggcaagttaa gttctcttgc accctgcgcg ctatttatcc
     1981 atgcatacat ttcagcgttg ctgtgaattg ccagtcgacg catcgcactg tttttttggg
     2041 cgctgatagt tttattactt ttcttaagca acgaaactat ctggttgatg ccccatcctt
     2101 tacctaatag ccgcagtacc ttgcgctctg aaagagtgag aatgacggag cgatagctaa
     2161 aatcatgagt ttcattcatt gccggagagg tcagcatggc gctgatacgc tctgcgtgcg
     2221 tattgccgga acgtacggtc ttgaccagat tctcaatggg ttcaacatca gacaataacg
     2281 tcgccgtagg gcacatgagc aattctactg cctgggaata ccaggagcgt gacaccatga
     2341 aaacccagtg aatttcccga tattgtgaga ttaaagaata ataatgttca caaacagaac
     2401 gtggatcttc actttcaccg gctaaatcag caatgactaa tccggaacga cgtaattgta
     2461 ataaggtaag gtcctccgca gaggcgctgg atattatttc atattcaggg aagtgagtcc
     2521 tcatgacctc ttttaaccct gcttgcataa caggtatttt actgataaca attccatttt
     2581 tgcagcatcc tggcaacata tttcctccac atccactatc taaaaatcat gatggtttat
     2641 tcaggatatt cattccttaa atgtttccag caaattaata gattaagcga ataaattatc
     2701 cttagcattc actttcatat cagcatactc ctgctgcaca acaactccac tgcatgtgtc
     2761 agaaggttag catcaaatga taacctagtg aaatagcctg gagacattta aaatatctac
     2821 attaagttgt taggaatact taatgaaaaa cagaaatcac attcatttaa tttataatta
     2881 aatgatatcc cttcccgcag aatgaaaagt cacacaacca atatttattc aatgaaatct
     2941 gatggtttta acttgttaac attaaaaaac gtgcccggtt atctattatc ctgattcgta
     3001 aaggccaaac aataaattca ttatagtgat aatattcatg tgatagtttg tttgcggcga
     3061 gagataattc gctttttatc accgagcttg tcccgataac caggattaat cagcgcaatt
     3121 ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata caaatggatt ctcattatct
     3181 gaacaatacg cagcacgtct atgacaaagg gcgagttatg caaactgagc aacagcgagc
     3241 cgtaacacgg ctttgtatcc agtgtggatt atttcttttg caacatggtg cggaaagcgc
     3301 gttggttgat gagctttcct cacgactggg tcgggcgttg gggatggaca gcgtcgaaag
     3361 ttctatctct tcgaacgcca tagtgctgac aaccattaaa gatgggcaat gcctgacatc
     3421 gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg gtgactgaag tccagcacat
     3481 tgtgattctg gcggagcacc atctgctgga ttacaaaggc gtagagaaac gatttagcca
     3541 aattcagcca ttacgttacc caagatggct ggtagcctta atggttggcc tttcttgcgc
     3601 ctgtttctgc aaactcaata aaggtggctg ggatggtgcc atcatcacct tctttgccag
     3661 tacggccgcg atgtatatcc gccagctgct ggcacaacat catcttcatc cgcagatcaa
     3721 cttttgcctc accgctttcg ccgccaccac catttccgga ttgcttttgc aacttcccac
     3781 tttcagcaat acacccacta tcgcgatggc cgccagcgtt ctgctgctgg tgccgggatt
     3841 tccgttgatt aatgccgtcg ccgatatgtt taaaggccac atcaataccg gactggcacg
     3901 ctgggcgatc gccagtctgc tgacactggc tacctgcgtc ggcgtggtga tggcactgac
     3961 gatttggggg ctacgcggat gggtgtgatc gagtttctgt ttgcattggc gcaggatatg
     4021 atcctcgccg ccattccagc ggtcggcttt gcgatggtgt tcaacgttcc cgttcgggca
     4081 ttacgctggt gtgcgctgct tggcgcgatt ggtcatggtt cacgaatgat cttaatgacc
     4141 agagggctga atattgagtg gtcaaccttt atggcctcca tgctggtcgg caccattggt
     4201 attcaatggt cgcgctggta tctggcgcat ccgaaagtgt ttaccgtggc ggctgttatc
     4261 cctatgttcc cgggcatatc ggcttatacc gcaatgattt cggcggtaaa aatcagccag
     4321 ttaggttaca gcgaaccgtt gatgattacc ctgttaacca actttcttac agcttcatcg
     4381 attgttggtg cgttatccat cggtctttcc attcctggat tatggttgta ccgcaagcgc
     4441 cctcgcgtat aaaattgcct cgcttaaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt acccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgatt
     4861 ttatccgcct ggcggcgcta tggggtgtgg cactaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gtcaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagaactact ggcctggcag aaagaacaag gagcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgg ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtacgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagtgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgcttc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtt cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccatcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccggcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac ctcaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc gatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctat aagatggcac aactctgggc tccggaactg gcactctcca
     8341 ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcaa catgttgaca
     8401 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttata
     8461 aaggcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8581 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccaaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aacccttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccacgtaagc
     8881 tcgggatcgg catggtggaa attaaagtgg tggaacgtga aggataattc ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacac cttctgcggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa acagcgccca acacttcccc ggacgctgca tggcaacttt aataatctcg
     9061 ccttatcaga aagtaattac acccttaatc aactcacgat tgttaatcac atcgcgctcg
     9121 taggtttctg ccagcgtggc gaacggatag cgatgggtta acatcatgtc agcagtgatt
     9181 tttccttccg ccatcagtcg accgacttta gcaaaatctt ccggcgtggc gttgcggcta
     9241 cccatcatcg tcgtttcttt tttatggaat tccggatcgg agaactgcaa ctcaccttta
     9301 aacaggccga caaataccac cgtgccgccg tgacgaatca aattcacggt gttattcatc
     9361 gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca gcgaaccacc aaactgcgcc
     9421 cgtagctgcg cgtcaaaatc ttcggctgac gggtccagta aaggtaattc cagacgcgtt
     9481 gccacatgtt cacggcgcgc cggactggta tccgccacca ccacctgtgc gccatcggct
     9541 ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc cgaccaccag cacctgctcg
     9601 ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa tagcgaaagg ttcaatcaat
     9661 gctgccgcct gcgggtcaat accgtctgcg ggcaaaatgt tcgccaccgg cacgctcaaa
     9721 tactcactaa aaccgccatc ctgatgcacg ccaatgactg aaattttttc acagcaattg
     9781 gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa cataagggat cacagcaact
     9841 tgcagaccat ttttaagatc agcaatattt ttacccagcc taacaatctc cccacatatt
     9901 tcatggccta aaacacgtgg ataactaaaa aatggttgat ttccacccca ggcatgaata
     9961 tcggtaccgc aaatcccgac agacttaatt tttattaatg cttcattgtc acccggaata
    10021 ggtatctcgc gttgtttcca gactaattct ttcggctgct ggcaaattaa aacattcatc
    10081 gtagacatga ttcggctctc cattttttgt tgccttagat atcaatgaaa atgaaaaatg
    10141 gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat aaaaatgttt taaatcgggt
    10201 tttaatggac tcacaaggag gaaaatatga gtcgttcaca aaatttacgc cacaatgtga
    10261 ttaaccaggt cattgatgat atggcgcggg gtcatatccc ttccccactt ccatcgcaaa
    10321 gtgcgctggc ggaaatgtac aacatcagcc gcaccacagt gcgtcatatt ctcagccatt
    10381 tacgcgaatg cggcgtcttg acgcaggttg gaaacgacta tgttattgcg cgtaaacctg
    10441 accacgatga cggtttcgcc tgtactaccg cttcaatgag tgaacaaaac aaagtgtttg
    10501 agcaggcttt tttcacgctg ataaaccagc gccagttacg cccaggggaa actttttctg
    10561 aactgcaact ggctcgagcc gcgggtgtca gcccggtggt tgtaagagaa tatcttttaa
    10621 aattcggccg ttataatctt attcaaagcg aaaagcgcgg ccagtggagc atgaagcaat
    10681 tcgatcaatc ctacgccgag cagctgtttg agctacgcga aatgctcgaa acacattcac
    10741 ttcagcactt tctcaacctg cctgatcatg atccccgctg gctgcaggcg aaaaccatgc
    10801 tcgaacgtca tcggctactg cgtgacaaca tcggcaatag ctttcgcatg ttctcgcaac
    10861 ttgacagaga ttttcactcc ttactgcttt cagctgccga caatatcttt tttgatcaat
    10921 cgcttgagat catctcagtc atctttcatt ttcactatca atgggacgaa agcgatctta
    10981 aacagcgcaa tatcattgcc gtcgatgagc atatgaccat tctcagcgct ctgatttgcc
    11041 gcagtgacct cgatgccacc cttgcgctcc gtaaccattt gaattcagct aaacaatcaa
    11101 tgattcgatc cattaatgag aacacgcgtt atgctcatta agtacggatt aaaaacagca
    11161 aactattagc gggtgcacgg ttttatcaaa agataaatac cgggaaattc ccgcttacct
    11221 atgctcac
//
LOCUS       DAJTYD010000127        11116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTYD010000127 DAJTYD010000000
VERSION     DAJTYD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.127"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            63..661
                     /locus_tag="OO585_002970"
                     /pseudo
     CDS             63..661
                     /locus_tag="OO585_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(690..1178)
                     /locus_tag="OO585_002971"
     CDS             complement(690..1178)
                     /locus_tag="OO585_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6630487.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1191..3998)
                     /locus_tag="OO585_002972"
     CDS             complement(1191..3998)
                     /locus_tag="OO585_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6630488.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            complement(4036..4140)
                     /locus_tag="OO585_002973"
     CDS             complement(4036..4140)
                     /locus_tag="OO585_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR6630489.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            complement(4149..4523)
                     /locus_tag="OO585_002974"
     CDS             complement(4149..4523)
                     /locus_tag="OO585_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6630490.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4579..5089)
                     /locus_tag="OO585_002975"
                     /pseudo
     CDS             complement(4579..5089)
                     /locus_tag="OO585_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(5089..6273)
                     /locus_tag="OO585_002976"
     CDS             complement(5089..6273)
                     /locus_tag="OO585_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCR6630491.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6431..7540
                     /locus_tag="OO585_002977"
                     /pseudo
     CDS             6431..7540
                     /locus_tag="OO585_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            7789..10908
                     /locus_tag="OO585_002978"
     CDS             7789..10908
                     /locus_tag="OO585_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6630492.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSNKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
BASE COUNT         3039 a         2783 c         2495 g         2799 t
ORIGIN      
        1 ttaaataatg gccgtttctg gcctgcatgg catgatttgc gcttttggac gggagattca
       61 gtgtgctgat tggctatgta agggtatcaa caaatgacca gaatacagac ctgcaacgaa
      121 acgctcttgt ttgtgcagga tgtgaacaaa tatttgaaga taaattaagc gggacaaaga
      181 cagaccgacc gggattaaaa cgcgctttaa agcgccttca aaaggtgaca cgctggttgt
      241 ctggaaactg gatcgcctcg ggcgaagcat gaaacatctg atttctctcg tcggggaact
      301 acgggagcga gggattaatt ttcgcagtct gaccgacagc atagacacat cttctccaat
      361 ggggcgtttt ttcttccacg tgatgggtgc cctggctgaa atggaacgtg aattaattgt
      421 tgaacgtaca ctggccgggc tggcggcagc gcgcgcaagg gggcgcacag gcggacgtcg
      481 accgaagctg acaaaagaac agcatgagca aatagcaagg ctaatcaaaa acgggcacga
      541 cagaaaacaa ctggcaataa tttacggcat tggcacatcg acgatttatc gttatcaccc
      601 tgtaggcgat atacagactg aagaaacaac cgggcagact caggaaaatg aaaaccgcta
      661 atctgaccat tagcggtttt tgtgttaaat cagaacagcc ctttaactga actggccgcg
      721 ctgttaagag atgatgtcac cttatctttg aagccggaca gcatatcact gaacgatgag
      781 gattgcaggc gctcccgcaa atcctcatca cagcgttcaa gagtcagtga aaattctatc
      841 tttttcgcct taccgtagcg atcaaactcg gagcgggtcg tattcgtttc agtcaggaca
      901 tacatgccgt aaatctgccc gacgccatca atcaaaggcc agggtcgtcc tgtatacgcc
      961 tgcgtagtca gcagcgacag cgacacttcg ccacctgtaa tttcaggata aagcacacca
     1021 gaaagaacga tgcgatcatc acctgcaccg atatactgcc agcttgctga acggttaacg
     1081 cgttcatttt tcacatgccg ccagcttttg ttttgctgta actgctgatg cggcagcgtg
     1141 cgcagctcaa aaacaaacat gccgtagatc atcatcatgg ccatgactcc tcaatcttta
     1201 tcgtaaaaac agccacgccc ggcacgggcg cgccgttcca tttctgccct gaccatttca
     1261 ccgaccagtt tcgccagttc gcggggattc tgcgtaacaa cgttatgcag atgaacatga
     1321 atttcaccac caaatccgaa ggcaacaggc tcccggttac gggaagttac aggaactgat
     1381 gccactggag atcgtatagc ctccgccacc gggcgggagc tggccgcaac aacagggacc
     1441 agcgccggag gcagcggagc cggaaccacg ggtgtgatat taattgcggg ggcaggctta
     1501 ctgacctgcg caatcttccg ctcctgccac tccccacgaa cagcaagtgc tcggggcagg
     1561 tttttaaaga caatatcgcc ggggccaatg cgttttttcg tctcctcaac cagcttacct
     1621 gtgttatcag caattttgct gagtctgcgc agcgtaccgg tattgctgtc tgtgagcggt
     1681 ttattgtctt tggggttatc acctccggtg ccattgccat tttccacagg cttcggcgga
     1741 ttgattttcg ccagatcccc ctgaagcaag gcaaccttgt cctgaagaat ggccgcacgc
     1801 tgtgcgtctt cgattttctt gcgcgccctt tccgcttcat ccggaagcac accaagcttt
     1861 tcaagtatcc acgccagcgt atccagcaac atttttgcag gtgtcagaac aagttgtaac
     1921 gcaccgccaa gaacgttacc gaatatctcg ccagcactgg tacatttatc cagagtttcc
     1981 ttgctggact ccatcggtga cagcagcgat ttaaaccagt taaacacctg gctgatccca
     2041 ctcccgattg cgtcaaaaac aggaccaaac cgttcaaagg tttcgcgcaa cggggtcagc
     2101 ctttccataa tcccgctgaa caccccggca aaaaatgccc tgatgggatc ccagtatttc
     2161 cagataagaa cggcagctcc ggcaagcgca gccacgataa gaccaaccgg actgaacaac
     2221 gccccgatag cgcctcccag taaagaaacg gaacccgtca ccattcccca tagtgctggc
     2281 agaaccctga cagcattcat tgatccggtc aggagggaaa aaccaagacg cagttttgcc
     2341 agcgggccag caagcacacc aatagccagc gacaacgagc caaccgttgc agtcattgcc
     2401 agcaatgcac cgcctgcaat cagtagctgg cgcgtcagtg cgggatgggc ctgcgccagc
     2461 gccgtcacct ttgataccac acgcgtgagc cactgcgtga cagaacgcag cggaccgtca
     2521 atcagatctg caatgcggat gcgcaaccct tcccatgcac tgctgagtga tttcagatcg
     2581 ccgtcaaggt tgttggccat aacctttgcc gtgcgttcag cctcaccgcg cgcgccttca
     2641 agttcttttc tcagtttggg taaggagccg tcacctgccg catcaacgag ggccataaat
     2701 gatgtgaaag cctcttctcc ggcaatgtcc ttaaagaacg atacccggtc aacttccccg
     2761 tatttgcggg tagctttata aaggtcagcc agcacatcct ccatcgggcg cattttgccc
     2821 ccggcatccg agacagacac gccaagctct ttcagcgcct ctgctgccgc ctttggcggt
     2881 gatgccagac gagccaggct ggcacgcatt gccgtaccag catcactccc cctgataccc
     2941 atattcgcca gcacgcccgc catcgctgcg gcctgctcca gagatattcc cagcttaccc
     3001 gccaccggac ctgcatattt catggtttca cccagtgcgc gaaggtcagt gttggtacgg
     3061 gtaaacgctg cggtgagtgt gtcgccgacc cggtccatct ggtcagcaga aagaccgaac
     3121 tgcgtcagaa tatttgagcc aatatccgcc gtctcaccga ggtccatacc gccagccgtt
     3181 gccatgctca gtacgccagg gagcgcagcc tgaatggcct gtggtgtaaa gccagccatt
     3241 gcaagaaatg cctgtccact ggcggcatcg cctgcggtga actgcgtttc agagccaagt
     3301 tttaacgcct gctcacgcag cgccttaaac tgcgggctgt tctggtcgat tcgcgtcagt
     3361 gcctgaacgc gggacatctc tttgccgaac ccgatcgcag gctgcaaaaa acgcccggca
     3421 gcatagccgc ccgccgctgc cgcaccaatt gccagcgcac cacctgtttt cagttttccc
     3481 gctgtttcct gcgcgcgcga ataccgctca cgcgcccgtg ttacacgcgc aagcgcctgc
     3541 cgttcgcgtt caagctggtt gttgtactgt tcggtgcgtc tgatggcctg ctggatggtg
     3601 ttatcgctgc ctgtcaggga aatgccgtgg cgtttcagct ctccgccaag ctcccgcatt
     3661 ttctgaattt cccgtgtgcg cgattcattc aggcgttcaa gccgggtgct taactgctgc
     3721 atcagctttt gttgtttttc gctgagcact gtacccgtgc gttgtaactg attaagggcg
     3781 ttaagctggc gtcgtgcttt cacgatgcca gcatccgctt tactgacagc gtcacgggcg
     3841 cgctcaaatg atcgcgcctg acgctcgaga tttttgatcg ccccctgcgt tcgctggatg
     3901 gagtcaccaa actgccccat caggcggcgg gcgttttcgg caggccgggt cagcctgtca
     3961 acggcgctga aagcgacccg gatatcaaga gtcttcattg tctgcattcc cgctacgaag
     4021 tgccgcccgc tcacgtcagc taaccacttc gccgggcgtc atcatgaaga tttcggcggg
     4081 cgaccagtta aaaatggcgg caatatccgc caccagatct tcgatgtgct caaagcacac
     4141 cagggtgatt acgctgccgt ctcctgcacg ctcttcgcgc cagagtctgg ctcgctcata
     4201 aaatttacag ccacagcgca caactgaata aaatcgcgtg acgacatttt tttaatcatc
     4261 acttcatcca gtcgtggcga ggtcacgcga ggcaacagcg tgaacatggt atccgctttc
     4321 agattcagca catcagacag cgacagacca cgcagggatc cagcctgctc aatagccccg
     4381 gtgatctcca catacgtgat tttttcgcca ccacgctcaa ttggtcgggt cagttttacg
     4441 ccacgttcga cagccatatc ctcaactgcc gtcacattat ccgccacggt gttattccgg
     4501 gtttcagtat cgatgtcttt catctgttgt ctccttttca gtcagaggcg acgcactgcg
     4561 ccgccagcat attacttatc agccaagccc aagcgcggaa cggatgcgat cgggcacaat
     4621 gtccttgccg tccttccggt aaatgaagtt cagcaggtca atctcccaca acgggcgatc
     4681 gttaacactc agcttgtagt aggtgtttta atggcgtaag tgtgtgatgt ggcttcgccc
     4741 tgtttggctt cccccatatc aatttccgtc acacgtccgc gcatttcgac ttcatacagg
     4801 tcgctttctg catcggtgta gtattcaccc gcaaaacgca gcagcgtgcc gtcaatcgtg
     4861 ccgccatact taaggaacag ctcacgaact gcgcccccat gacaaagctc gcatcaagcg
     4921 cggagtcgtc cagaccgaga tcaatactta ccgcacccat catgccacca ccccggtagc
     4981 tgtcggtttt gcgcgtcagc ttaggcagag tgacggacgt cacattaccc acttcgtttt
     5041 caccatccac aaacagcgta aaaaagcgaa gatgttttgg cacagccatc aggcacctcc
     5101 cagcaccgca aatgcgggtt caaagtattc atcagtaaac gtctggtaaa gctccatgtc
     5161 ttccagtggc ggaacgggcg tatatttgta gcgaatacgc acacgtccct gacgtaaatc
     5221 cgtggtgctg ttatccacca cgtcatacca gcactccgcg ccaatcagtt tcccggcagt
     5281 caccagtgaa tccagttttg ccctgatggc actgataaca tccttcacat tcgcaggcgt
     5341 cagtggactg tcgatggttt caaactgcgc ttccgcaatt gaatcagcca gcacctgtgc
     5401 ggttcgggta tacacctcaa agatgtaggc gttcgtttcc ggtgtgcggt taccccagaa
     5461 gcggaacccg ttgcgacgaa taatggtcgt gatttctttg ttgttgaggc tgttggcatc
     5521 gctgtcttcg gcctgcaacg accagaacac atgcctcgac atccccagca catttttaac
     5581 cggaacgttg gacagcgatt tgtgccatcc ctgttcatgg tcaatgtacg cacgaaggcc
     5641 gcacgcatag gcaggcgcgg ggaacgtttc gtttttgcca tttttcgggt tgtaggcgat
     5701 gaagtccggc cataagagca tcacctcacg ttcgttgaat ttctggcggt aggtaattgc
     5761 ctcggccatc gtgttacagc catgacatga ggcatacaca aacgcgcgca gtttatctgc
     5821 aatcacgcac agggattttg ttaccgcctc cgtgtccagc tccggcgcgg ccagaatacg
     5881 cggacggtat ccgatgcttt catcctgctc tgcaacaagc agcgcataca tccccgtata
     5941 gctgccgtca tcctcagaac caccgataac cagttgatcc tgcgtctttc cgtcttcttc
     6001 tttgtgttca gccacgcgaa cgacgatcac ctttgtgctc acctggtctg cgatggcctt
     6061 aagcgcacga taaagcgtcc ccgttgtccc gcattttccc agcacgtcat tgacgcgggt
     6121 cagcagtgtg ggcttgttca gcgggaacag cttcgcgtcc gcatcatccg ccgttgccac
     6181 gataccgata acgctggaat caacatcgtt aatcgctgtt accaggtcgg tattctccgt
     6241 aacacgggca ccatgaaaac gagtttcact catagcttca gccccttgta tccgttaaat
     6301 gattcagcaa caatcatcac ccaccacgcg cgtaatctca cccctgcgcc gttctcccgc
     6361 cacggcgaca acaaaaagca gtaaccccct ccgcacgcac atgcgaccat gccgcacagg
     6421 gagggagcag atgaccgaca ccaccatgca attgctcagt cagggcacgg cccccgtgaa
     6481 aatgccggat tttgatattc tctcggaggg taaaacgctg tcaggcgtgg cggagcgcct
     6541 gatgagcctg taactgaccg acaaccgggg atttgaggcg gaccagctca ccatcacgct
     6601 ggatgatgcg gatggtcagt tgcagctacc gccacggggc gcgcgtctga cggttctcat
     6661 tggctggaaa ggggaaccgc tgacagaaaa aggcacttac attgttgatg aaatcgctca
     6721 cgaaggaccg ccggacaggc tgactgtttc agccagaagc gcagattttc gggatgaatt
     6781 taacgttaaa cgtgaggtgt cctggcatga tgtgaccgtt gagcgtgtgg tatccgccat
     6841 cactcatcgg tacggtctga aaccgcaaat cagcgaaatg ctgatggata tcgaaatcga
     6901 ccacgccgac cagaccgaag aaagcgacat gtcctttctt acgcgcatgg cggaaatgct
     6961 gggagcaatc gccacggtaa aaagcggcaa tctgttattc atcatgccag gtggtggcgt
     7021 gaacgcacag gggcagccgt tgccctcgtt cgccattaca cgcagcagcg gcgatcgcca
     7081 tcagttccgc attgctgacc gcgaggcgta tacgggggta cgcgcctact ggcttgatct
     7141 taattacggg aaaaagaaaa aagtcagcgt gaaacgccgc aaaccgccaa aacccaaaaa
     7201 ggagaaaagc agcagtcggg aaggcgacta tatggaaggt gcggaaggca acgtgtttgt
     7261 gttacgcaag acttatcaga acgagcaggc agcaagacgc gcagcggcgg caaagtggca
     7321 gcagctacaa cgcggagccg catcattctc catcacgctg gcgcgtggtc gtgcagaact
     7381 ctatcccgaa atgcatggca cagtgacagg ctttaaaagc gagattgatt atcaggactg
     7441 gactattgca aaagccgagc acaccattga taacagcggc tttaccacgc agcttgaact
     7501 tgaagcaaaa atcccggaat ggatagcaga aacagaataa atctcttaaa aatattattt
     7561 ttgagatgtg cttctacact gacagtaaga acaaaagata atgtatcagc ccactagatg
     7621 tcagaggttg gggcatcaac cagatgctaa caataatacg gatgtttttc ccacatgaca
     7681 gaggaagtca agtataatct tcacagtcca tcccgtccaa gggaatatga ctcaatagta
     7741 aaagttccag tttagttagt agacccacac tttatagaag agtaattcat gattggacaa
     7801 tttttaagtg caacagagat tttagcaaag aactatgttc gtaataaaat ggttaaaaat
     7861 ccattttatt cgaatttaaa atggaatttt attgaaaaaa acatcataag attaacatcc
     7921 tcaccggtta aatcagtact ttgcatatca gctttttcat ttgtactact ttatgttgga
     7981 tatttaaacg aattatttat aaaaaataat cttttacatt atttcccgtt taggcattca
     8041 ttaaccgaat ggcaaacaac cattttaagt ggccaattga caattattgg tattgtatac
     8101 ccccttgtta taggtttagt aagtgtctta ttccaaaaaa aggcagatag aaaaatagcc
     8161 caaactgctt atcagcgtta ctcaggattc atgcttgctg gactcagcgg gcttttctta
     8221 tcaggattta tacttctcag tgtactaatt aaaactgtct ttggaagtta tctttatggt
     8281 atagcttgtc taatcagcat attatggctt ttgataaaca tagttctttc tatttggttc
     8341 tttatcgtga gtcttgaaat tcttgatgat gtaaaacggc aaataattat aaagcgatac
     8401 attgccttcg aaatagtaat gccccatatt tgtaacaaaa tatcagccaa gcttaggtta
     8461 taccctatct atcaaaaaca taactattca aatttagaaa tcacgcaagc cgattacaaa
     8521 ggagaataca tatctgtggc tagcagttat tctaaggaag atgagttaag tttatatcac
     8581 cgtccttttc aacttactct taatcttatt aattaccaac taaaaaagaa gaatcatttt
     8641 gcgtcatttg tcattggtga taatcgtaca aaagaaacag aatccacggg taaaatactt
     8701 tttagcgtta aaaatatcaa accagacagc ttattaataa aaatacttaa gcaatgtttt
     8761 tatagggctc cgattaaagg aggagatttt tccgtaagtc ttacaatgca ggctataaca
     8821 gcagatacgt atatgtatct gcgggattct gatctcatta gttttgacaa tgctatctct
     8881 gcattaatta ataactttaa taatctctgc gatttgtatt tttttcaaga tgataatacc
     8941 aacaataact tcttactgat cactacagaa ttatttgaac ggagttttca atatgagttt
     9001 tcagatgaag tttataaaat atcaaataat tccatggata aaattaatct ttctgaaaga
     9061 ttttttgaat tatgtctttg gagcggagtg cgtattatca ataatagaaa acatcttatc
     9121 agcaatgaac tttgtatcta tatgggaatt actcgttcac aatggtcaat attaacagaa
     9181 tggttccgaa acaaccaatc actcttaaat gcctcgcttc gttcacgtta taatcgtata
     9241 ttaagaacct atatcactgt ttgggagcaa tatcaagaaa gcataaactt tcgtttctgc
     9301 aatacagaga attcagattt atttgaatta ttttgcaaaa cgcaactaca agaacttcca
     9361 tcgatgatta ttgatgcaac acagactcgc gatccttcta ctattgatac agccgtcgat
     9421 cttattaacc gatggcagca ctcaatgaat attgacagcc attcggtaga aaaatacagt
     9481 tacaaaggcc agctttttaa cccgggtttt ttcatatcca aaaaactaaa cttcaattca
     9541 gacagagagt ggttcaatat tgccatcata aatgcgttga ctgacatgcg tatatgcaca
     9601 tgtctttatc tgacatcaag aatcaatact tctgacaaac taatgacaca ttacattaaa
     9661 cttattttag agggtaaact tattgaccaa accggtggat atgaaactcc cactgaagaa
     9721 attgataatg caagccaact aatcaagata ttaattagaa tctgcctgtg gacatggtct
     9781 gaaaatatgg agcacaatgg gtggatgaat agtttagcaa ggcgtttacg tgactatgat
     9841 aaaacagaca tggtgatggg cagagtttac agtaatgttt ttgactgtgg atttattgat
     9901 atggaacaat catgggttca gctattatta attttttcta acaagaacga tagcgtatca
     9961 aaagaaataa aagaagcaat agaaaacaac tatataacat atcgtgagaa gcaacgactc
    10021 attggagtat tatcaaaaat ttgtaactcc atcgaataca ctaaaatcaa gctgacttta
    10081 acattggacg atctccaaac aaaaaaagaa aacttaagaa agttgcttca agagcatatt
    10141 aacatgctaa agaaagatct tgatatgaga cttcaggatg ctgccataga tgtacatcga
    10201 ctagatagca ctgcacgtaa aacatcagaa catcttcgta aacgcatcaa gaaaacgctc
    10261 cctctttcac ttttcaaatc tattgatttt aagcaggcta gcgattgttt cacaaaacat
    10321 aagatatcta ttaagataga taaagaaccg tatgccgaag gaatagaatc catcccttat
    10381 atcaatgaag gtgatattca agcagactta attctcaagg acatccagag aataatactt
    10441 tcaaatttat ttagcactgg ctgttcgcaa cataccgtaa tagaagattt caatatgttg
    10501 attgatcaca taaaatcatc tgctgacttg gctggcaaac tggttcttgt gatgagtaaa
    10561 gagatctttc agcaatataa tcgtatgctt ttcgataatc ccaacctcag agaactaatg
    10621 agaaaaaatg atgatggctc tatgaacata actactgaat ccggtacccg taaggtttac
    10681 ttcttaccat ttgttaatca accattttct cttgtggtaa aggataacta cttcactaaa
    10741 ttaattatta gagaatatga taataataag ttagtaaatg tcacttcaga gaatatcaaa
    10801 agtgactcag ataaattcaa gctaactctt aactatgagt tgaatatagt atttgaagga
    10861 aacgctgatt taaaaatagc tcactcgcaa agagtgacat cagaataata tctttgacgg
    10921 cgctccttag agtgccgttc gtttattaac taatgctaaa aatcattata tagagaaagt
    10981 catgtttaaa tcttagagaa tacaacctag aatagtgtaa gcactacaat aagagaggtc
    11041 gctatgttcc gttgtccgct ttgtggcgca tctgcccgta tccgcaccag tcgtccggaa
    11101 aatgattcaa acaccg
//
LOCUS       DAJTYD010000128        11016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTYD010000128 DAJTYD010000000
VERSION     DAJTYD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.128"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..771
                     /locus_tag="OO585_002979"
     CDS             <1..771
                     /locus_tag="OO585_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6630493.1"
                     /translation="PTPFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWN
                     MLNYVALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPS
                     GIKMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLN
                     KAGFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVI
                     SCSETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            1619..3187
                     /gene="cydA"
                     /locus_tag="OO585_002980"
     CDS             1619..3187
                     /gene="cydA"
                     /locus_tag="OO585_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6630494.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            3203..4342
                     /gene="cydB"
                     /locus_tag="OO585_002981"
     CDS             3203..4342
                     /gene="cydB"
                     /locus_tag="OO585_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6630495.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            4357..4470
                     /gene="cydX"
                     /locus_tag="OO585_002982"
     CDS             4357..4470
                     /gene="cydX"
                     /locus_tag="OO585_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6630496.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            4470..4763
                     /gene="ybgE"
                     /locus_tag="OO585_002983"
     CDS             4470..4763
                     /gene="ybgE"
                     /locus_tag="OO585_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6630497.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            4913..5317
                     /gene="ybgC"
                     /locus_tag="OO585_002984"
     CDS             4913..5317
                     /gene="ybgC"
                     /locus_tag="OO585_002984"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6630498.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            5314..6006
                     /gene="tolQ"
                     /locus_tag="OO585_002985"
     CDS             5314..6006
                     /gene="tolQ"
                     /locus_tag="OO585_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6630499.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            6010..6438
                     /gene="tolR"
                     /locus_tag="OO585_002986"
     CDS             6010..6438
                     /gene="tolR"
                     /locus_tag="OO585_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6630500.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            6503..7684
                     /gene="tolA"
                     /locus_tag="OO585_002987"
     CDS             6503..7684
                     /gene="tolA"
                     /locus_tag="OO585_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6630501.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAVKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            7817..9109
                     /gene="tolB"
                     /locus_tag="OO585_002988"
     CDS             7817..9109
                     /gene="tolB"
                     /locus_tag="OO585_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6630502.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLVFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            9144..9665
                     /gene="pal"
                     /locus_tag="OO585_002989"
     CDS             9144..9665
                     /gene="pal"
                     /locus_tag="OO585_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6630503.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            9675..10466
                     /gene="cpoB"
                     /locus_tag="OO585_002990"
     CDS             9675..10466
                     /gene="cpoB"
                     /locus_tag="OO585_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6630504.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            10631..10706
                     /locus_tag="OO585_002991"
     tRNA            10631..10706
                     /locus_tag="OO585_002991"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10664..10666,aa:Lys,seq:ttt)
     gene            10742..10817
                     /locus_tag="OO585_002992"
     tRNA            10742..10817
                     /locus_tag="OO585_002992"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10775..10777,aa:Val,seq:tac)
     gene            10820..10895
                     /locus_tag="OO585_002993"
     tRNA            10820..10895
                     /locus_tag="OO585_002993"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10853..10855,aa:Lys,seq:ttt)
BASE COUNT         2718 a         2583 c         2945 g         2770 t
ORIGIN      
        1 cctacacctt ttaacaccga cagtgttctg gcagatacgc agtttggttc gctaacgcgc
       61 cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt
      121 ccggtatgga atatgctcaa ctatgttgcc ttacaggaag ggcgtaacgg catggctgtc
      181 tttagcgaag ggttacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
      241 acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgcttttaag gcctgggcgg
      301 ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc
      361 ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga
      421 gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac
      481 aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc
      541 ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat
      601 ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc
      661 tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg
      721 gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc
      781 aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga
      841 tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat
      901 gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt cactctcaag
      961 ccacgtatat gtggctttat ttttaacaaa ataataaccc tgggtgagtt aattataata
     1021 taattataag ttaattaaat gttaatatcg gcgggggtgg atttatgcct ttattagtaa
     1081 tcctgaaact ctgcgtcgta ttagccagtg accaaaaaaa gaattaaggt caaccgtgct
     1141 gtttttgctt cgtctctttt tatctttaat tgccaaccga aactaatttc agccttataa
     1201 ctcacacatt ttaaacataa atgtcactaa agttacctta ttgaaacatg attaacataa
     1261 tttgtaggaa ttgatattta tcaatgtata agtcttggaa atgggcatca aaaagagata
     1321 aattgttctc gatcaaattg gctgaaaggc ggtaatttag ctataaattg atcaccgtcg
     1381 aaaaatgcaa atttgcttca acaaaaacct gtttattgta aggattttgc ggcgtaatat
     1441 atacgtggga tcaatttgag tttttattaa catgtttgca acctttcttt acgccgtttt
     1501 tgtgtgcatt cacatggtat gatgaaagtg ttcaaacaaa tttctattgg ggcatgcgtg
     1561 tgaccctttc taacggggtt cactctcgga gtcttcatgc gatgagcaag gagtcatgat
     1621 gttagatata gtcgaactgt cgcgcttaca gtttgccttg accgcgatgt accacttcct
     1681 ttttgtgcca ctgacgctcg gtatggcgtt cctgctggcc attatggaaa cggtctacgt
     1741 cctctccggc aaacagattt ataaagatat gaccaagttc tggggcaagt tgtttggtat
     1801 caacttcgct ctgggtgtgg ctaccggtct gaccatggag ttccagttcg ggactaactg
     1861 gtcttactat tcccactatg taggggatat cttcggtgcg ccgctggcaa tcgaaggtct
     1921 gatggccttc ttcctcgaat ccacctttgt aggtctgttc ttcttcggtt gggatcgtct
     1981 gggtaaagtt cagcatatgt gtgtcacctg gctggtggcg ctcggttcaa acctgtccgc
     2041 actgtggatt ctggttgcga acggctggat gcaaaaccca atcgcgtccg atttcaactt
     2101 tgaaactatg cgtatggaga tggtgagctt ctccgagctg gtgcttaacc cggttgctca
     2161 ggtgaaattc gttcacactg tagcgtctgg ttatgtgact ggcgcgatgt tcatcctcgg
     2221 tatcagcgca tggtatatgc tgaaaggtcg tgacttcgcc ttcgctaaac gctcctttgc
     2281 tatcgctgcc agcttcggta tggctgctgt tctgtctgtt attgttctgg gtgatgaatc
     2341 cggctacgaa atgggcgacg tgcagaaaac caaactggct gctattgaag ccgagtggga
     2401 aacgcaacct gcgcctgctg cctttactct gttcggcatt cctgatcagg aagaggagac
     2461 gaacaaattt gcgattcaga tcccttacgc actgggcatc attgcaacgc gttccgtgga
     2521 taccccggtt atcggcctga aagagctgat ggtgcagcat gaagaacgca ttcgtaacgg
     2581 gatgaaggcg tactctctgc tcgaacaact gcgttctggt tctaccgacc aggcggttcg
     2641 tgaccagttc aatagcatga agaaagacct cggttacggt ctgctgctga aacgctatac
     2701 gccaaacgtg gctgatgcga ctgaagcgca gattcaacag gcaactaaag actccattcc
     2761 gcgtgtagcg ccgctgtact tcgcgttccg tattatggtg gcgtgtggct tcctgctgct
     2821 ggcaatcatc gcgctctctt tctggagtgt catccgcaac cgcattggcg agaaaaaatg
     2881 gctgctgcgc gccgcgctgt acggtattcc gctgccgtgg attgctgtag aagcgggctg
     2941 gttcgtggct gaatatggcc gccaaccgtg ggctatcggt gaagtgctgc cgacagctgt
     3001 ggcgaattcg tcactgaccg caggcgatct catcttctca atggtgctga tttgcggcct
     3061 gtataccctg ttcctggtgg cagaattgtt cttaatgttc aaatttgcac gcctcggccc
     3121 aagcagcctg aaaaccggtc gctatcactt tgagcagtct tccacgacta ctcagccggc
     3181 acgctaagac aggagtcgtc aaatgatcga ttatgaagta ttgcgtttta tctggtggct
     3241 gctggttggc gttctgctga ttggttttgc agtcactgac ggtttcgaca tgggggtggg
     3301 catgctcacc cgtttcctcg gtcgtaacga caccgagcgt cgaattatga ttaactccat
     3361 tgcaccacac tgggacggta accaggtttg gctgatcacc gcgggcggcg cactctttgc
     3421 tgcctggccg atggtctatg ccgctgcgtt ctccggcttc tatgtggcga tgatcctcgt
     3481 gctggcgtct ttgttcttcc gtccggtcgg ttttgactac cgctccaaga ttgaagaaac
     3541 ccgctggcgt aacatgtggg actggggcat cttcattggt agcttcgttc cgccgctggt
     3601 aattggtgtt gcgttcggta acctgttgca gggcgtaccg ttcaacgttg atgaatatct
     3661 gcgtctgtac tacaccggta acttcttcca gttgcttaac ccgttcggcc tgctggcagg
     3721 cgtggtgagc gtagggatga tcattactca gggcgcaacc tatctgcaaa tgcgtaccgt
     3781 gggcgaactg cacctgcgta cccgtgcaac ggctcaggtg gctgcgctgg tgacactggt
     3841 ctgtttcgca ctggctggcg tatgggtgat gtacggtatc gatggttatg tcgtgaaatc
     3901 gacaatggac cattacgcag cctctaaccc actgaataaa gaagtggttc gtgaagctgg
     3961 cgcatggctg gttaacttca acaacacgcc aattctgtgg gctattccgg cactgggtgt
     4021 ggttctgccg ctgctgacca tcctgactgc acgtatggat aaagccgcgt gggcgtttgt
     4081 gttctcctcc ctgacgctgg cctgcatcat cctgacagcc ggtatcgcaa tgttcccgtt
     4141 tgtgatgccg tccagcacca tgatgaacgc aagtctgaca atgtgggatg caacttccag
     4201 ccagctgacg cttaacgtca tgacctgggt tgcggtggtt ctggtaccga tcattctgct
     4261 ctacaccgcc tggtgttact ggaaaatgtt cggtcgtatc accaaagaag atattgaacg
     4321 taacacccac tctctgtact aagtaaggag ctaaaaatgt ggtatttcgc atggattctg
     4381 ggaacgcttc ttgcctgttc gtttggggta atcaccgcgc tggcgcttga acacgtcgaa
     4441 tcaggcaaag ccggtcaaga agacatctga tgagtaagat tatcgcgacc ttgtatgcgg
     4501 taatggacaa gcgccccctg cgggcgcttt ccttcgtgat ggcgcttctg ttagcaggat
     4561 gtatgttttg ggacccatct cgttttgccg cgaagaccag tgaactggaa atctggcatg
     4621 gtttattgct gatgtgggcc gtctgtgctg gtgtgattca cggcgtgggc tttcgtccgc
     4681 agaaggttct ttggcaaggg attttttgcc cattgcttgc cgatattgtt ctcattatcg
     4741 ggctgatttt cttcttcttt taaatcagaa ttctctttaa aaaattatgg gccgctccag
     4801 gcccataaat ttttacgctc ccttaacttg ccctcattcc caaacctcaa tcgcgcgcgt
     4861 atagtagcag cgtttaaaag ctctaacttt tgttgcatta ccgggatgta aagtgaatac
     4921 aacgctgttt cgatggccgg ctcgcgtcta ctatgaagat accgatgccg gtggtgtggt
     4981 gtaccacgcc agttacgtcg ctttttatga aagagcacgc acagagatgc tgcgtcatca
     5041 tcacttcagt cagcaggcgc tgatggctga acgcgttgcc tttgtggtac gtaaaatgac
     5101 ggtggaatat tacgcacctg cgcggctcga cgatatgctc gaaatacaga ctgaaataac
     5161 atcaatgcgt ggcacctctt tggttttcac gcaacgtatt gtcaacgccg agaatacttt
     5221 gctgaatgaa gcagaggttc tggttgtttg cgttgaccca ctcaaaatga agcctcgtgc
     5281 gcttcccaag tctattgtcg cggagtttaa gcagtgactg acatgaatat ccttgatttg
     5341 ttcctgaagg ctagccttct ggttaaactt atcatgttga ttttgattgg tttttcaatc
     5401 gcatcctggg ccattattat ccagcggacc cgtattctta acgcagcggc gcgcgaagcc
     5461 gaagcgtttg aagataaatt ctggtctgga atcgaactct ctcgcctcta tcaagagagt
     5521 caggggaaac gggataatct gactggttcg gaacaaatat tttacagcgg gttcaaagag
     5581 tttgtgcgcc tgcatcgtgc caatagccat gcgccggaag ccgtagtgga aggggcgtcg
     5641 cgtgctatgc gtatctccat gaaccgtgaa cttgaaaatc tggaaacgca cattccgttc
     5701 ctcggtacgg ttggctccat cagcccgtat attggtctgt ttggtacggt ctgggggatc
     5761 atgcatgcct ttatcgccct cggggcggta aaacaagcaa cactgcaaat ggttgcgccc
     5821 ggtatcgcag aagcgttgat tgcgactgca attggtctgt ttgccgctat cccggcagtt
     5881 atggcctaca accgcctcaa ccagcgcgta aacaaactgg aactgaatta cgacaacttt
     5941 atggaagagt ttaccgcgat tctgcaccgc caggcgttta ccgttagcga gagcaataag
     6001 gggtaagtca tggccagagc gcgtggacga ggtcgtcgcg aacttaagtc cgaaatcaac
     6061 attgtaccgt tgctggacgt actgctggtg ctgttgctga tcttcatggc gacagcgccc
     6121 atcatcaccc agagcgtgga ggtcgatctg ccagacgcta ctgaatcaca ggcggtgagc
     6181 agtaacgata atccgccagt gattgttgaa gtgtctggta ttggtcagta caccgtggtg
     6241 gttgagaaag atcgcctgga gcgtttacca ccagagcagg tggtggcgga agtgtccagc
     6301 cgcttcaagg ccaacccgaa aacggtcttt ctgatcggtg gcgcaaaaga tgtgccttac
     6361 gatgaaataa ttaaagcact gaacttgtta catagtgcgg gtgtgaaatc ggttggttta
     6421 atgacgcagc ctatctaaac atctgcgttt cccttgcttg aaagagagcg ggtaacaggc
     6481 gaacagtttt tggaaaccga gagtgtcaaa ggcaaccgaa caaaacgaca agctcaagcg
     6541 ggcgataatt atttcagcag tgctgcatgt catcttattt gcggcgctga tctggagttc
     6601 gttcgatgag aatatagaag cttcagccgg aggcggcggt ggttcgtcca tcgacgctgt
     6661 catggttgat tcaggtgcgg tagttgagca gtacaaacgc atgcaaagcc aggaatcaag
     6721 cgcgaagcgt tctgatgaac agcgcaagat gaaggaacag caggctgctg aagaactgcg
     6781 tgagaaacaa gcggctgaac aggaacgcct gaagcaactt gagaaagagc ggttagcggc
     6841 tcaggagcag aaaaagcagg ctgaagaagc cgtaaaacag gccgagttaa agcagaagca
     6901 agctgaagag gcggcagcga aagcggcggc agatgctaaa gcgaaggccg aagcagatgc
     6961 taaagctgcg gaagaagcag cgaagaaagc ggctgcagac gcaaagaaaa aagcagaagc
     7021 agaagccgcc aaagccgcag cgcagaaaaa agccgaggca gccgctgcgg cactgaagaa
     7081 gaaagcggaa gcggcagaag cagctgcagc tgaagcaaga aagaaagcgg caactgaagc
     7141 tgctgaaaaa gccaaagcag aagctgagaa gaaagcggct gctgaaaagg ctgcagctga
     7201 taagaaagct gcagccgaca aaaaagcagc agaaaaagcg gctgctgaaa aggcagcagc
     7261 tgataagaaa gcagcggcag aaaaagccgc cgcagaggca gatgatattt tcggtgagct
     7321 aagctctggt aagaatgcac cgaaaacggg gggaggggcg aaagggaaca atgcttcgcc
     7381 tgccgggagt ggtaatacta aaaacaatgg cgcatcaggg gccgatatca ataactatgc
     7441 cgggcagatt aaatctgcta tcgaaagtaa gttctatgac gcatcgtcct atgcaggcaa
     7501 aacctgtacg ctgcgcataa aactggcacc cgatggtatg ttactggata tcaaacctga
     7561 aggtggcgat cccgcacttt gtcaggctgc gttggcagca gctaaacttg cgaagatccc
     7621 gaaaccacca agccaggcag tatatgaagt gttcaaaaac gcgccattgg acttcaaacc
     7681 gtaatcgcga tgttgactgt tcggacggtc aacatcaggc accggttgcc acggggttct
     7741 ggtagttttg tgtattttag tttgttaaca ttctgctaaa ttatcgtggg ccatcggtcc
     7801 agataaggga gatatgatga agcaggcatt acgagtagca tttggttttc tcatactgtg
     7861 ggcatcagtt ctgcatgctg aagtccgcat tgtgatcgac agcggtgtag attccggtcg
     7921 tcctattggt gttgttcctt tccagtgggc ggggcctggt gcggcacctg aagatattgg
     7981 cggcatcgtt gctgctgact tgcgtaacag cggtaaattt aatccgttag atcgcgctcg
     8041 tctgccacag cagccgggta gtgcgcagga agtacaacca tctgcatggt ccgcactggg
     8101 cattgacgct gtagttgtcg gtcaggtcac tccgaatccg gatggttctt acaatgttgc
     8161 ttatcaactt gttgacactg gcggcgcacc gggtactgta cttgctcaga actcgtacaa
     8221 agtgaacaag cagtggctgc gttatgctgg tcataccgcc agtgatgaag tgtttgaaaa
     8281 actgaccggc attaaaggtg cgttccgtac ccgtattgcc tacgttgttc agaccaacgg
     8341 cggtcagttc ccgtatgaac tgcgcgtatc tgactatgac ggttacaacc agtttgtcgt
     8401 tcaccgttca ccgcagccgc tgatgtctcc ggcgtggtca ccagacggtt ctaaactggc
     8461 ttatgtgacc ttcgaaagcg gtcgttccgc gctggttatt cagacgctgg caaatggcgc
     8521 tgtacgtcag gtggcttcat tcccgcgtca caacggtgca cctgcattct cgccagacgg
     8581 cagcaaactg gcattcgcct tgtcgaaaac cggtagtctg aacctgtacg taatggattt
     8641 ggcttctggt cagatccgcc aggtgactga tggtcgcagt aacaataccg aaccgacctg
     8701 gttcccggat agccagaacc tggtatttac ttctgaccag gccggtcgtc cgcaggttta
     8761 taaagtgaat atcaacggcg gtgcgccaca acgtattacc tgggaaggtt cgcagaacca
     8821 ggatgcggat gtcagcagcg acggtaaatt tatggtaatg gtcagctcca atggtgggca
     8881 gcagcacatt gccaaacaag atctggcaac gggaggcgta caagttctgt cgtccacgtt
     8941 cctggatgaa acgccaagtc tggcacctaa tggcactatg gtaatctaca gctcttctca
     9001 ggggatggga tccgtgctga atttggtttc tacagatggg cgtttcaaag cgcgtcttcc
     9061 ggcaactgat ggacaggtca aattccctgc ctggtcgccg tatctgtgat aataattaat
     9121 tgaatagtaa aggaatcatt gaaatgcaac tgaacaaagt gctgaaaggg ctgatgattg
     9181 ctctgcctgt tatggcaatt gcggcatgtt cttccaacaa gaacgccagc aatgacggca
     9241 gcgaaggcat gctgggtgcc ggcactggta tggatgcgaa cggcggcaac ggcaacatgt
     9301 cttccgaaga gcaggctcgt ctgcaaatgc aacagctgca gcagaacaac atcgtttact
     9361 tcgatctgga caagtacgat atccgttctg acttcgctca aatgctggat gcacatgcaa
     9421 acttcctgcg tagcaacccg tcttacaaag tcaccgtaga aggtcacgcg gacgaacgtg
     9481 gtactccgga atacaacatc tccctgggtg aacgtcgtgc gaacgccgtt aagatgtacc
     9541 tgcagggtaa aggcgtttct gcagaccaga tctccatcgt ttcttacggt aaagaaaaac
     9601 ctgcagtact gggtcatgac gaagcggcat actccaaaaa ccgtcgtgcg gtactggttt
     9661 actaagagaa ttgcatgagc agtaacttca gacatcaact attgagtctg tcgttactgg
     9721 ttggtatagc ggccccctgg gccgcttttg ctcaggcacc aatcagtagt gtcggctcag
     9781 gctcggtcga agaccgcgtc actcaacttg agcgtatttc taatgctcac agccagcttt
     9841 taacccaact ccagcaacaa ctttctgata atcaatccga tattgattcc ctgcgtggtc
     9901 agattcagga aaatcagtat caactgaatc aggtcgtgga gcggcaaaag cagatcctgt
     9961 tgcagatcga cagcctcagc agcggtggtg cagcggcgca atcaaccagc agcgatcaaa
    10021 gcggtgcggc ggcatcaacg acgccgacag ctgatgctgg tactgcgaat gctggcgcgc
    10081 cggtgaaaag cggcgatgca aacacggatt acaatgcagc tattgcgctg gtgcaggata
    10141 aatcccgcca ggatgacgca atggtggcat ttcagaattt catcaaaaat taccctgatt
    10201 caacttacct gccaaacgcc aattattggc tgggtcagtt aaactacaac aagggtaaaa
    10261 aagatgatgc ggcgtactat tttgcttcgg tagtgaaaaa ctatccgaag tcaccaaagg
    10321 ctgctgatgc gatgtttaaa gtcggcgtca tcatgcagga caaaggtgac accgcaaaag
    10381 cgaaagccgt ataccagcag gttatcagta aataccctgg tactgatggc gctaaacagg
    10441 cacaaaaacg tctgaacgcg atgtaatgca taacacacga ccagaagttg cattatttct
    10501 ggtcgtgtcg tgcgaatcat aagcagttga gtgatctgca tcgaaatttt tgttgcgctc
    10561 aagtctgaaa tcagtaatat atgccgccgt tgccacggga tatcaaacaa accgaaagca
    10621 acgaaaaagt gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc
    10681 aggttcgaat cctgcacgac ccaccactaa catagttagt tgtagtatcc agcgtagtat
    10741 tgggtgattg gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat
    10801 cccgtcatca cccaccaccg ggtcgttagc tcagtcggta gagcagttga cttttaatca
    10861 attggtcgca ggttcgaatc ctgcacgacc caccactaat tacgatgggt tcggtggaag
    10921 tagtttgtag tatccagcgc agtatcgggt gattagctca gctgggagag cacctccctt
    10981 acaaggaggg ggtcggcggt tcgatcccgt catcac
//
LOCUS       DAJTYD010000129        10928 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTYD010000129 DAJTYD010000000
VERSION     DAJTYD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.129"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            229..885
                     /gene="pphA"
                     /locus_tag="OO585_002994"
     CDS             229..885
                     /gene="pphA"
                     /locus_tag="OO585_002994"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR6630505.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(886..1077)
                     /locus_tag="OO585_002995"
     CDS             complement(886..1077)
                     /locus_tag="OO585_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6630506.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1182..1418)
                     /locus_tag="OO585_002996"
     CDS             complement(1182..1418)
                     /locus_tag="OO585_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6630507.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1536..2975)
                     /gene="rsmF"
                     /locus_tag="OO585_002997"
     CDS             complement(1536..2975)
                     /gene="rsmF"
                     /locus_tag="OO585_002997"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6630508.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3055..5688)
                     /gene="yebT"
                     /locus_tag="OO585_002998"
     CDS             complement(3055..5688)
                     /gene="yebT"
                     /locus_tag="OO585_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6630509.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5657..6940)
                     /gene="yebS"
                     /locus_tag="OO585_002999"
     CDS             complement(5657..6940)
                     /gene="yebS"
                     /locus_tag="OO585_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6630510.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7070..7567
                     /gene="msrC"
                     /locus_tag="OO585_003000"
     CDS             7070..7567
                     /gene="msrC"
                     /locus_tag="OO585_003000"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6630511.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7664..8362
                     /gene="proQ"
                     /locus_tag="OO585_003001"
     CDS             7664..8362
                     /gene="proQ"
                     /locus_tag="OO585_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6630512.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8382..10430
                     /gene="prc"
                     /locus_tag="OO585_003002"
     CDS             8382..10430
                     /gene="prc"
                     /locus_tag="OO585_003002"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6630513.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10622..>10928
                     /locus_tag="OO585_003003"
     CDS             10622..>10928
                     /locus_tag="OO585_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="metalloprotease; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6630514.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     "
BASE COUNT         2805 a         2717 c         2817 g         2589 t
ORIGIN      
        1 gattaaaaga tgaacgtaaa ttcattcaac gcattaattt taaagggttt tattgtttgt
       61 tgacgaaaac aggaatcgtg ttcggtctct ttttatctgt taaaagccag aagcatttcc
      121 ttcgctgact ttatagtcaa ccataacaca cactctactg tctgagtcca gcgtttttta
      181 acattcttgt taagattatg tgatctttag cacgggagga aaatattgat gaaacagcct
      241 gcgcccgttt atcagagaat tgcgggtcat caatggcgac atatctggct ttctggcgat
      301 atacacggtt gtcttgagca gttgcgccgc aaattatggc attgtcgttt tgatccgtgg
      361 cgagatttac ttatctcagt gggagacgtt atcgatcgtg ggccgcaaag tttacgttgt
      421 ctgcagttac tggaacaaca ttgggtttgt gcggtaagag gcaatcatga acagatggcg
      481 atggatgcgc tggcatccca gcagatgtct ttgtggttga tgaatggcgg cgactggttt
      541 attgcgctgg cagataatca acagaaacaa gcgaaaacgg cgctggaaaa atgtcagcat
      601 ttgcccttta ttcttgaagt acacagtcgc accggcaagc atgttattgc tcatgcggat
      661 tatccagatg atgtttatga atggcaaaag gacgttgatt tgcatcaggt cttgtggagc
      721 cgctcgcgat taggtgaacg ccaaaaaggg cagggaatta caggtgctga tcatttctgg
      781 tttggtcata caccgttgcg acatcgcgtg gatattggca acctgcatta tattgatacc
      841 ggtgctgtct ttgggggcga actgactctt gtgcaattgc aatgattaaa aatcaccgta
      901 ctcctgtgca ggtcgccaga aaccatctat aaaatcctca atcggaaaac aaccgccctg
      961 gcggatccgt tgatcgctca tagaataaag acactgctgt tccgtgttgt agacatccac
     1021 aacaatatct tcacaaccgc catccaggta gcaaacaaaa agtaccagcg cgaacatttc
     1081 atccccgaag tgtggtgccg taccgttaag tttaggagag attttacaac gggggaataa
     1141 ccaggacaaa taacccgcca tgccgacggg ttctttttgg atcaggcaag acgcataatc
     1201 caggcatcag attgttgatc gtaacgggta cggtaaagga cagagtgttc gccatttagc
     1261 agggcaggga tactggtttc agcatcccag gcatcgagtt gcatacataa atcggggtca
     1321 gatttacaag gaatggttaa cgttcgatca cccggcgatt caccgtgtaa ttcgccgtcg
     1381 tcgatttcaa aagcgcctat gcgcacactg gttttcatct tcgtgctctc catttagctg
     1441 ccggttgtgg tacgaccagt ccgatcgcac catttttcag cctagttgag gcagccgaat
     1501 gtgccagtca aaaagtgcgc tttttttgtg cgctgtcagg cgttaccggt aaaaagtttc
     1561 ccatcgcgca ccagttcacg cggatagctg tttttcaatc gcgaaccaat ccgtttggct
     1621 aaaccaatcg gttgatgctg gaaagtaacc aacacgtcat ccgccactgg cgcggcttgc
     1681 gggtaaacat cgcgcccgcg ataccactcc tccgcttcct gcggtgtcag ttcaaaagcg
     1741 ttcacattgt cgggggtggc aagggcaata actgcttcat gctgccagcg ataacctttg
     1801 ttgtgcgttt cggcaagttt aatccccaag cgagaaaatc ggactttacc gatcagggct
     1861 tcaatgccca ccgggaacaa ccacagttct ttgtcacgct gccagaggcg caggttttca
     1921 tcccagttta agccaacacc tgcagccgcc tggcgaattt gtccagcttc gcgatctttc
     1981 accgggctga acggaaaatt accgactttg tatttggggg cgggtaaggc gggaatcgcc
     2041 tgagttttac gcagacgagc aacgaagaag ccttcgcagt cgtaaatttg tgggaaaaca
     2101 tgcaaaaagc cttcttcggt cagcgctttg tttgcaccag ggaagagatc gccaagtggt
     2161 aaaaactcta ctgcgtcggg gtaagtctct ttcagccaca ggcaaacggc ttcgttttct
     2221 tcctggttta aggtacaggt cgagtaaacc agcgtaccac caggacgtaa tgcatgaaag
     2281 gcgctgtcga taagctcccg ttgtgtagct gcgatttcct gattgctttc tggtgaccag
     2341 ttttttagcg catcgggatc tttacgcacc acgccttcgc cggagcaggg agcgtccagc
     2401 aaaatggcat caaacatttc tggcactgcc gcaccaaaca cgcggccatc aaaatgtgtg
     2461 agcgcaacat tactgatgcc acagcggctg atattggcat gtaacacttt tacccgactg
     2521 gcggaaaact cattggcaag gattgcccct tcgttattca tccgcgcggc aatttgcgtc
     2581 gtttttgagc ctggcgcagc agcgacatcc atcacccgct gtggtgcatt accgtcagca
     2641 aacaaggcgg cgacgggcaa cattgaactg gcttcctgaa tataaaacag gccacttaaa
     2701 tgctcggcgg tactacccaa tggcaatgca tcttcattgt cgcgttcaat ccagaaacct
     2761 tcttcacacc acggaattgg cgtaagcgtc cagccataag gagcggttaa ttgcaggaaa
     2821 tcagcaacgg agattttcag cgtattaacg cgaatgctgc ggcgcaacgg gcgctgacag
     2881 gcggcaagaa aatcatcaaa tgagagcgtc gaaggcatag cttcgcgcat ttgtgtcaga
     2941 aacgcgtccg ggaaataaac ggtgtgttgg gccacgagca tgtaccactg gcaaaaaaac
     3001 aggcgcgcag tttagcataa acgctccggc gcaggcacgc cggagcagtg ggcattattt
     3061 gggaagcgca gttccccatt cacgccactc tttcggttca ctttcctgca acaggaagtg
     3121 tttgccttcc tgggctttcg gtgccagtgg cgtacccgga ggcgtggcga aggcgatgcc
     3181 gccacggata aactggttaa aggtgccggt tttcactacg ccgcccgtca gaccaaagtc
     3241 cagactgtaa cccgatgcca accagaagac ggaattgtta cgcaccaggt gttgatagcg
     3301 tttactgatg cgcatcgcaa tcatcacgcg atctgacaat gtccccagcg tcattccggt
     3361 aaccgtacct acttccagac cacggaacag cacaggcgta ccaataccta acgaaccggc
     3421 ttccggtgct tcgacaataa tgcttaagcc atccaggtaa cgcgaatcag taatagtagc
     3481 ttcttgcaac tcaaagtcac ggcgaggatt accccgacct ggttcgacat tgatatacgg
     3541 ctggaggata gtatctagat gctcaacgcc cgtggctgaa atttgcggtg tgaccaccga
     3601 gaagcgcgta ccgccgcggg cgaaggtctg gacatactcc ggatagagca ccgcctttgc
     3661 ctgtacttca ttgcgcgcgg taatcagatc cagcgtctgg atttgcccga tatcaatacc
     3721 gagatagcga attggcatcc cgaccgccag ttttccggca tcgaaagcgt gaagcgtaat
     3781 ctgcccacta accgcacggg ccgctgtttc ggaagcatac agaattcgtt tgtcgccttt
     3841 acgctgactg gcgctggcac cgctgaggtt atcgaagcta atggctccct ttaatgctct
     3901 ggagagcggg gatgcctgta cggtcaggcc actaccattc agctgaactt tcgccccgcc
     3961 ttctgcccag aacacgctat tgctggtcag aaggttgcga tactccggct taatatgcag
     4021 atcgatatca aacgcgttag ctcgcggacg cacggtaatc acttcaccaa cttcaaattt
     4081 acggtacagc actaccgatc ctgcctgcac atccggcagc gtctctgcac tcaaactcac
     4141 ggttgtggtg ggtaaatcgc taaggctgtt ctccagcgct ttttccagat tggcatacag
     4201 tggatagctg gctttcatct cacctttatc gcctggcaga atacgtatcc cgccgttaat
     4261 ccattctgag gcgctggcac caagaaactc aacgccatcc agccccacct tcacgtcgac
     4321 acggctgttg acgacaaatt tgctatcgcc ttttaccagt tcgcgatgct gaggctcgat
     4381 ggcgacggta aaggtaacgc ctttgctggt gagtttacga tcaataacct ggcctacctg
     4441 cacgccgtga agaatgagcg gctgacccgt atcaataccg taactttccg gcgcggtcag
     4501 ggtcagcgtc agaacatcag gttcatgcag cagtgctttt tcgcctggca caacaacgaa
     4561 ctctttgcgt ggctcgccat cgccgggtac caactcgaag gttttgccag tcagcagggc
     4621 gctgagattg gcatcgctaa gggataattt cggattacgt aattcgatgc gggtattttc
     4681 acgaagcagg gtaacgacgc tgggatcaac tgtcatttcc ccagtgactt taccaccagg
     4741 atttaaatcc agtttagtta gctgtccgac ttccagtccc tgatacatta atggcgtcga
     4801 gtcggcggtt aatccggccc cactcggcag ttccagtttg attattacgc cacgctggct
     4861 atgggcgagg tcttcataca gaccaaaggt atcttccgcc tcggcaggtt tcgactcttc
     4921 tggtgaatcg aaggcaatcg caccgttaac cagtgccgcc aggctttcca gtttcacctt
     4981 cgcgccactg atactgacat tggcatcaac gccggaaacg ttccagaaac ggctaccttt
     5041 tttcaccaga tcggtaaaac gccgctcgat caggacatca atcaccacgc cttgtttgtt
     5101 gggattgatg gcatagtcgt agacttttcc caccgggatc ttgcggaaat agaccaatga
     5161 accgctgttc agcgaaccga gatcgggggc ttgcaggtgg atcatcagat cgccattgtt
     5221 cagccgatat ttcggttggg tatcgagtgc gacaaagtga tcctgctctt tacctgtacc
     5281 cggcatcatg ccgatatagt tcccaccgac gagggcgtcc agcccggaga cacctgccaa
     5341 cgatgctttt ggcgtcacca gccagaattg agtctcttcg cgcagcgcat ctttcatatc
     5401 ggacttgatg ctgaccttga cttcaatctt acgaagatcg tcgctgaggc tgatatcctg
     5461 cactgttccg acttcaacgc cctgataacg aacaggcgta cggcccggga caataccatc
     5521 cgccgacata aagtcgatgg tgacggtatt acttcggtcc tgataactgt cccaaatcag
     5581 ccaaccggca atcattagcg cgatgaaagg cagcagccag aaaggtgaga tacggcgttt
     5641 atttttaatc tgcgcttcag tcgtcgaagc gggcgtttcc tgactcatgt gcatcccaaa
     5701 gtaagcggct gtccagccat tccacagcaa gaatagtcaa aattacggct gcgccaaaat
     5761 aaaacgcagc cggtcccata gtaaaagcga ggatctgatc gcgattaatc agcgacatgg
     5821 ttaaagatat gacaaacagg tcgagcatcg accagcgtcc aatccaggtc accatccgca
     5881 gtaacagaat gcgtgtgcgt aaaccttgct ggcatttaaa atgaatgctc aacagtaaag
     5941 tgaacatgac gatcacttta gtaaacggta ccagaatact ggcgataaac acgattccgg
     6001 cgaccgcaat gttgctactt gccagcgaca taatcccgga aagaatcgta tcttcctgcc
     6061 ggccaccatt cagataaatg atagaaatag gcaataggtt ggctggtaac aacaaaacga
     6121 ttgacgctaa cagcgccgcc cagcattttt gcaaactatg acgacgacgc aggcgtagcg
     6181 ggatatggca acgcgggcag cgaccacgct gatcaggata gccggtaaaa tggcacccaa
     6241 gacagacacg aagtttctcg tccctacgcg tagcggggcg ctgcggataa tatcgctccc
     6301 acagctcctc aacattaaga tgtgacaacg tcaccgtcgt taaaatcacc aacgccacaa
     6361 aagagaacaa gccgacaccc gcctggatat gggcataatc ctgtaccttt atagaagcaa
     6421 cgccaatgcc gaccaggtag atatccagca ttacccactc tttaagacgc tcaagcatca
     6481 gcagtaccgg acgtaaattc attcccagtc ggttaccaaa ccataaatat gctatggagg
     6541 acaccagaat gaggggggca ccaataacgc agaaaaagac catcgccccc gttatcgtat
     6601 cgccctgttt ggtcatttgc cagatgcctt gcatgacgtt tgcgtcgata cgaataccta
     6661 acagccagat atgcaacagc ggttcgcccc aggcaaacgg catcagcaac agcatggtga
     6721 aagccattgc cgccaggcgc gttagcgacc agtcgcgccc gtcgcgaatt tttgcctgac
     6781 agcgcggaca ataggcactc tgatgagaat ttatctcggg caggctaaac agcatgtcac
     6841 attgcgggca acgttggtaa tcaccacgcg gcagttcctc gccgattgac ctcactgtta
     6901 tcttttttgt cggcgtaatt tgtggtgtgt taagagccat gacgtgccaa atgtaataag
     6961 aattaacatt aatcttaacc catgatggga atgatcttga gcattaacgc atttaatgct
     7021 tattttaata ggctggcagt tccaggtaag acaaacaagt gattatataa tgaacaaaac
     7081 agaattttac gcggatttaa atcgcgactt taacgcgctg atggcgggag aaaccagttt
     7141 tctggcaacg cttgcgaaca ccagtgcgtt gttatacgag cgtctcactg acgtaaactg
     7201 ggcaggtttt tatttgcttg aggacgatac actggtactc ggaccatttc agggcaaaat
     7261 tgcctgtgtc cggatacccg tcgggcgcgg ggtgtgcggc actgcggttg cccgcaatca
     7321 agtgcagcgt atcgaggatg ttcacgcgtt tgacgggcat attgcctgtg atgcggcgag
     7381 taattctgaa attgttctgc cgctggtggt gaaaaatcag attattggtg ttcttgacat
     7441 cgatagtacc gtcttcggtc gctttacaga cgaggacgag caaggcttac gtcagcttgt
     7501 ggcacagctt gaaaaagtgc ttgcaacgac ggattacaaa aaattctttg cgagcgtcgc
     7561 aggataatca acggataacg tagcatttac tggtagcgtc attataatga cgcctgttca
     7621 tgcctgcgct tgttggctac gtccgttgta atcaggaaat ttcatggaaa atcaacctaa
     7681 gttgaatagc agtaaagaag taatcgcgtt tctggccgaa cgttttcccc actgtttcag
     7741 tgcggaaggt gaagcgcgtc cgctgaaaat cggtattttt caggatttgg tcgatcgtgt
     7801 tgccggggaa atgaacctga gcaaaacgca attacgatcc gctttacgtc tctacacttc
     7861 gagctggcgt tatctttacg gtgttaaacc cggcgcaacg cgtgtcgatc ttgacggcaa
     7921 cccatgcggt gagctggacg agcaacatgt agagcatgct cgcaagcagc ttgaagaagc
     7981 gaaagcgcgt gttcaggcac agcgtgctga acagcaagcg aaaaaacgcg aagctgccgc
     8041 agctgctggt gagaaagaag acgcaccgcg ccgcgaacgc aagccacgtc cgaccacgcc
     8101 acgccgcaaa gaaggcgctg aacgtaaacc tcgtgcgcaa aagccggtag agaaagcgcc
     8161 aaaaacagta aaagcacctc gcgaagaaca gcacaccccg gtttctgaca tttcagctct
     8221 gactgtcgga caagctctga aggtgaaagc gggtcaaaac gcgatggatg ccaccgtatt
     8281 agaaatcacc aaagacggcg tccgcgtcca gctgaattcg ggtatgtctt tgattgtgcg
     8341 cgcagaacac ctggtgttct gaaacggagg ccgggccagg catgaacatg ttttttaggc
     8401 ttaccgcgtt agctggcctg cttgcaatag caggccagac cttcgctgta gaagatatca
     8461 cgcgtgctga tcaaattccg gtattaaagg aagagacgca gcatgcgacg gtaagtgagc
     8521 gcgtaacgtc gcgcttcacc cgttctcatt atcgccagtt cgacctcgat caggcatttt
     8581 cggccaaaat ctttgaccgc tacctgaatc tgctcgatta cagccacaac gtgctgctgg
     8641 caagcgatgt tgaacagttc gcgaaaaaga aaaccgagtt aggcgatgaa ctgcgttcag
     8701 gcaaactcga cgttttctac gatctctaca atctggcgca aaagcgccgt tttgagcgtt
     8761 accagtacgc tttgtcggta ctggaaaagc cgatggattt caccggcaac gacacttata
     8821 accttgaccg cagcaaagcg ccctggccga aaaacgaggc tgagttgaac gcgctgtggg
     8881 acagtaaagt caaattcgac gagttaagcc tgaagctggc aggaaaaacg gataaagaaa
     8941 ttcgtgaaac cctgacacgc cgctacaaat ttgccattcg tcgtctggcg caaaccaaca
     9001 gcgaagatgt tttctcgctg gcaatgacgg cgtttgcgcg tgaaatcgac ccgcatacca
     9061 actatctttc cccgcgtaat accgaacagt tcaacactga aatgagtttg tcgctggaag
     9121 gtattggcgc agtgctgcaa atggatgatg actacaccgt tatcaattcg atggtggcag
     9181 gtggtccggc agcgaagagt aaagctatca gcgttggtga caaaattgtc ggtgttggtc
     9241 aaacaggcaa gccgatggtt gacgtgattg gctggcgtct tgatgatgtg gttgccttaa
     9301 ttaaagggcc gaagggcagt aaagttcgtc tggaaatttt acctgctggt aaagggacca
     9361 agacccgcac tgtaacattg acccgtgaac gtattcgtct cgaagaccgc gcggttaaaa
     9421 tgtcggtgaa gaccgtcggt aaagagaaag tcggcgtgct ggatattccg ggcttctatg
     9481 tgggtttgac agacgatgtc aaagttcaac tgcagaaact ggaaaaacag aatgtcagca
     9541 gtgtgatcat cgacctgcgt agcaatggcg gtggggcgtt gactgaagcg gtatcgctct
     9601 caggtctgtt tattcctgcg ggtcccattg ttcaggtccg cgataacaac ggcaaggttc
     9661 gtgaagacag cgataccgac gggcaggtgt tctataaagg cccgctggtg gtactggttg
     9721 accgtttcag tgcttcggct tcagaaatct ttgccgcggc aatgcaggat tacggtcgtg
     9781 cgctggttgt gggtgaaccg acgtttggta aaggcaccgt tcagcaatac cgttcattga
     9841 accgtattta cgatcagatg ttacgtcctg aatggccagc gctgggttct gtgcagtaca
     9901 cgatccagaa attctatcgc gttaacggcg gcagtacgca acgtaaaggc gtaacgccag
     9961 acatcatcat gccgacgggt aatgaagaaa cggaaacggg tgagaaattc gaagataacg
    10021 cgctgccgtg ggatagcatt gatgccgcga cttatgtgaa atcaggcgat ttaacggcct
    10081 ttgggccgga gctgctgaag gaacataatg cgcgtatcgc gaaagatcct gagttccaga
    10141 acatcatgaa ggatatcgcg cgcttcaacg ctatgaagga caagcgcaat atcgtttctc
    10201 tgaactacgc tgtgcgtgag aaagagaata atgaagatga tgcgacgcgt ctggcgcgtt
    10261 tgaacgaacg ctttaaacgc gaaggtaaac cggagttgaa gaagctggat gatctaccga
    10321 aagattacca agagccggat ccttatctgg atgagacggt gaatatcgca ctcgatctgg
    10381 cgaagcttga aaaagccaga cccgcggaac aacccgctcc cgtcaagtaa tatcaatcag
    10441 gcacaagaaa ttgtgcctga ttttttaaca gcggcaagat gccgtaaatc agatgctaca
    10501 aaatgtaaag ttgtgtcttt ctggtgactt acgcactatc cagacttgaa aatagtcgcg
    10561 taacccatac gatgtgggta tcgcatattg cgttttgtta aactgaggta aaaagaaaat
    10621 tatgatgcga atcgcgctct tcctgctaac gaacctggcc gtaatggtcg ttttcgggct
    10681 ggtactgagc ctgacaggga tacagtcgag cagcgttcag gggctgatga tcatggcctt
    10741 gctgttcggt tttggtggtt ccttcgtttc gcttctgatg tccaaatgga tggcattacg
    10801 atctgttggc ggggaagtga tcgagcaacc gcgtaacgaa agggaacgtt ggctggtcaa
    10861 tactgtagca acccaggctc gtcaggcagg gatcgctatg ccgcaagtgg ctatctacca
    10921 tgcgccgg
//
LOCUS       DAJTYD010000130        10887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTYD010000130 DAJTYD010000000
VERSION     DAJTYD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.130"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            296..1171
                     /locus_tag="OO585_003004"
     CDS             296..1171
                     /locus_tag="OO585_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6630515.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1179..1955
                     /locus_tag="OO585_003005"
     CDS             1179..1955
                     /locus_tag="OO585_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6630516.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2124..4385
                     /locus_tag="OO585_003006"
     CDS             2124..4385
                     /locus_tag="OO585_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6630517.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4454..5629
                     /gene="senB"
                     /locus_tag="OO585_003007"
     CDS             4454..5629
                     /gene="senB"
                     /locus_tag="OO585_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6630518.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6088..6438
                     /locus_tag="OO585_003008"
     CDS             6088..6438
                     /locus_tag="OO585_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6630519.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6581..6802
                     /locus_tag="OO585_003009"
                     /pseudo
     CDS             6581..6802
                     /locus_tag="OO585_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6823..6885)
                     /locus_tag="OO585_003010"
                     /pseudo
     CDS             complement(6823..6885)
                     /locus_tag="OO585_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7336..8748
                     /gene="ospC1"
                     /locus_tag="OO585_003011"
     CDS             7336..8748
                     /gene="ospC1"
                     /locus_tag="OO585_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6630520.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(8852..9000)
                     /locus_tag="OO585_003012"
                     /pseudo
     CDS             complement(8852..9000)
                     /locus_tag="OO585_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9076..10773
                     /gene="ospD3"
                     /locus_tag="OO585_003013"
     CDS             9076..10773
                     /gene="ospD3"
                     /locus_tag="OO585_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6630521.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKI"
BASE COUNT         3385 a         2295 c         2296 g         2911 t
ORIGIN      
        1 ttttcttcga catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt
       61 cagactccgg atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg
      121 gagtgaccat agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc
      181 gctagtgcat tctttttgtt ggtaatgata attatttaca tttattcttt gatgttttat
      241 cataattaat gttttatcaa taaatatatg gaaatatatt gtttcagatg ggaatatgag
      301 aaagttcatc cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca
      361 gaatgtcacc ataaagcagg atgcgccagg gcagaaagct ttcctgattg acgggcagat
      421 ttacgatttg cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac
      481 cgctcgacat cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct
      541 ttggcttatt caaaatctgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt
      601 gacgtcagac caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt
      661 ggttcgagat agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat
      721 gtatggcgac atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat
      781 tggccgggag cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc
      841 cactgcacct gtggtacaag aagctctgcg tgaaaccatc atagctatgc atgaaggcaa
      901 ccttgaaagc cagcaactga cgtcaatgct ctctgttcag cagcagcggg atcgctacat
      961 ggcccgccag cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc
     1021 gtcaaaaaac atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt
     1081 ggtgttgatg cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg
     1141 gtttgtagcg ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttcc
     1201 cacagtcggt aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt
     1261 tccttacgtt tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga
     1321 tcatgctgca atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg
     1381 gaatcgctca acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac
     1441 aacgtcctgt cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat
     1501 ccgatggaga aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca
     1561 gcaaccccac agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta
     1621 ttgctgcgcc ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa
     1681 gcagagtaaa aggccacctt aaccgttata aacgttaccc tggcgatgcc agaaaacgtg
     1741 cacgaaccgg cacagccgtt gtgactttta ccgtaaacac ggaaggcact attgtcagta
     1801 gtttcttgga aatctcgtca ggaacccttt ctcttgaccg cgaagctatt gctgttttag
     1861 aacgcgctca accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag
     1921 taaaaatgcc gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagttca
     1981 tttaactgaa atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc
     2041 gaaaaaatca aaaagacagt agatcataat taataaacaa atagttatac ttatctttat
     2101 taatattata tccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat
     2161 tattgctcag ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa
     2221 aaggagcggc agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg
     2281 aagctctcga gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac
     2341 ttgacgcaac agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag
     2401 caatcagtgt gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg
     2461 gtattaccca gagtttttac ggaacctcta cctccggaac aacggcgcat ggttcaacta
     2521 acaatatggc tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg
     2581 gtgacagcag tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta
     2641 ttggcgttga cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg
     2701 tcggtagtaa tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt
     2761 ttacggatac gggaagcatt ggcgttatgg ctgctgtgag tggcagttct gtgtactcta
     2821 atttctcaaa tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc
     2881 agaaccccaa atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg
     2941 aactttccgc tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact
     3001 attacattaa atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca
     3061 gtaccagtcg cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct
     3121 cagcgcaaaa tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg
     3181 acaatgaact ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca
     3241 ttcactcagc ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg
     3301 caccctccgg ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca
     3361 tctgggaggc agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg
     3421 cctgcgattc acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg
     3481 gtggcttcaa cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca
     3541 ttggctacag caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag
     3601 gaggcgcatc catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt
     3661 ttaacattga taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg
     3721 cgtaccgcag caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag
     3781 gtcgtaaatg cagtatggct gaggtgattg gcaatgactg ggagggcatt agcgatgaat
     3841 acagcgacaa tatgtacatc tacgttaact cggcaagcga cgttattgca aagggcttcg
     3901 aactggagat ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc
     3961 aaacagacca gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac
     4021 tgcccagaaa atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc
     4081 tgggcactat cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg
     4141 acgaacatac cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc
     4201 tctatggtac ttacgagtac aaccgcaacc tgacactgaa actttcggta caaaacctga
     4261 tgaacagaga ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg
     4321 agagccaccc agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt
     4381 tctgatgaca atttccgcca ggttcagaca atacgttttc tctcttatgt caattttatt
     4441 gcaggaacga aaaatgaata ttttcacttt atccaaagca ccgctatact tgttaatttc
     4501 actattttta cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt
     4561 tgccctgaaa acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga
     4621 taatgccagt aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa
     4681 aggctatctg tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc
     4741 ataccgggca ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac
     4801 ccagtcagcg tccctgcgta tcagtatgtt tgacacccca accggtaaac tggtaagaac
     4861 cagcgacagg ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcatttgcg
     4921 ccatatggtt tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa
     4981 aacggccgag ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc
     5041 cttaaaaggc gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga
     5101 aaaaacacag aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa
     5161 tcagacgctg ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca
     5221 gaatctggcg gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga
     5281 tcgttccggt acaaaagatg gcctctatat ttttgactta cgcgacggta aacagcttgg
     5341 ctatgtgcac acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact
     5401 gtatgtcacc aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat
     5461 cacccgtgaa tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga
     5521 taccctttac attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt
     5581 ggaaggagct aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cgggggaaaa
     5641 aacatgggat aaaaactccg ttattccact aaactggccg gactaggaaa agcaaacatc
     5701 tctgtcctgc cggtacgcct gttacgccgc tccatgaggc tgaggaatct ccagaaaacg
     5761 agacaggcac agcattgtac gatcaactga tttgtgccaa taattcaatg aaatcacagc
     5821 tatgcctgtt cacgtatagg aaattgaaaa actaagcgat accactgtcc gcgtcttatg
     5881 cctcgtgcgg taagctgttt ttcacatcac gccggtgttt ttttctttct gtggacggtt
     5941 ttaatactgt aatcggccgt tttttatact taccccgatt cccgggcgca cttatcctgc
     6001 atcaccccgg gatatacctc agcagactga tatcccgcgc gtttttcttc agcccctcca
     6061 cattcagtcc cgcgatggcc cggcggtata ccatccggcc gccacacagc acgcattcga
     6121 acgggtcacg actcaggaac tgtttcacca tttgtgcata gcacactttc gccactggtt
     6181 ccggtttatc ctgtttttat aacggtaatt gcagaccatg acaaaccgca gccgtcaggc
     6241 tgcctactcg agtcacaagg ctctctgtgt ccgatatgcc ggcagcgcat tacccatgaa
     6301 actggctgga acgtacacca taaggtgaag aaagttatgg ggggcggtga agaattatct
     6361 aaccttgtct tgttacatcc aaattgccat cgacagttac acagttgtga agccggctct
     6421 ctcaaaaagg gcttataaag gcttgagcgg tgtaccggga aaccggtatg caccgttctt
     6481 aggggagggc agtccagcaa tgaactgtcc ttacccgaca aacatccaaa acgaacggaa
     6541 cgtgctggaa tccgcatata acccgttgta aagtgaggaa atggacacct cacttgctca
     6601 tgagaacgcc cgcctgcggg cactgttgca gacgcaacag gacactatcc accagatggc
     6661 tgaatacaac cgcctgctct cacagcggat ggcggcttat gcttccgaaa tcaaccgact
     6721 gaaggcgctg gttgcgaaac tgcagcgtat gcagttcggt aaaagcccag aaaaacttcg
     6781 cgcaaaaacc gaacggcaga ttccattttc acgggcgatc tacgccacac aggctctggg
     6841 ttgcagtgac tgctccatta aggcgatttt gaactcataa gggaagtaac aaaaaaactg
     6901 caaaagtaca ataattatat atggccaaac taacctctat taaaaaaaca tcagtttttc
     6961 acacttagtt gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca
     7021 ctaactggaa gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt
     7081 cattgctgtt caataaaatt gatataatta ttaaattatg gaaataattt cacaaaaaag
     7141 attaacttga gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa
     7201 catcaaaagt atataatacc cttatctaat tacatgcagt atattgtata tatactcaaa
     7261 aattgtaatt cataccctac tatatcttgt ggggaaagat attaattaaa actgttttca
     7321 tataaggttc attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat
     7381 atagattcta tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac
     7441 gctgcacaac aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa
     7501 gatttcttta ggaaaactat agcagcacaa agttatagta gaatgttctc tcaaggctct
     7561 aactttaaat ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag
     7621 gctattgagc accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt
     7681 catcctcttt ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc
     7741 ttcagacatc aaagtaattc tgatttgtct gataaaattt taaacattaa gtcattcaat
     7801 aaaattcagt ctgaaggaat atgcacaaaa cgaaacacat acgctgatga tataaaaaaa
     7861 atagctaatc atgactttgt gttttttggc gttgaaatct ctagccatca gaaaaaacac
     7921 cccctgaata caaaacatca cactgttgat tttggtgcaa atgcgtatat cattgatcat
     7981 gactctccat atggatatat gacattaacc gatcactttg ataatgctat tccacctgtt
     8041 ttttaccatg agcaccaatc atttttagat aaattttcag aggttaataa agaagttagt
     8101 cgatacgtac atggaagtaa aggaattata gatgtaccaa tattcaatac taaagatatg
     8161 aagttagggc tcggattata cctgattgac tttattagaa aaagtgaaga ccaaagcttc
     8221 aaggagtttt gctatggaaa aaatcttgcc cctgtggatc tggatagaat cataaacttt
     8281 gtttttcagc cagagtacca tatacctagg atggtaagta cagaaaactt caaaaaagtt
     8341 aagattagag aaatatcctt agaggaggct gttacagcat ctaattacga agaaattaac
     8401 aagcaggtca ctaacaaaaa aattgctctc cagactcttt ttctttcgat tactaatcaa
     8461 aaagaggatg tcgccttata tatattatct aattttgaga taactagaca agatgttatt
     8521 tccataaagc atgagttgta tgatattgag tatctactta gcgctcataa ttcaagctgt
     8581 aaagtacttg agtattttat caataaggga ttggttgatg taaacacaaa gttcaaaaaa
     8641 actaatagtg gggattgtat gttggataac gcaataaaat atgagaatgc agaaatgata
     8701 aaactattat tgaaatatgg tgcaacatct gacaataaat atatttaatc aaaattgaat
     8761 atcgtttagc aaaaggcagt atttgcacaa aatatcgata tattgtttat tgtcagtatg
     8821 gctcaatgtg ataatagttg gaaagtttga tgggtttcgc cccgttgtag cggtagtcga
     8881 gctggaaggt cttcaggcac tgcttacagc gatagagcag cccccctgaa ctggaatggc
     8941 cgttccggat accccctgag tttcagagta acggggacaa accacatcaa tctttgccat
     9001 caatcatcca aagggcaaag agtacaacaa cactaagtct gcgtcacaac ccatcaatga
     9061 aaggaatata tacatatgcc atcagtaaat ttaatcccat caaggaaaat atgtttgcaa
     9121 aatatgataa ataaagacaa cgtctctgtt gagacaatcc agtctctatt gcactcaaaa
     9181 caattgccat atttttctga caagaggagt tttttattaa atctaaattg ccaagttacc
     9241 gatcactctg gaaaacttat tgtctgtcga cacttagctt cctactggat agcacagttt
     9301 aacaaaagta gtggtcacgt ggattatcat cactttgctt ttccggatga aattaaaaat
     9361 tatgtttcag tgagtgaaga agaaaaggct attaatgtgc ctgctattat ttattttgtt
     9421 gaaaacggtt catggggaga tattattttt tatattttca atgaaatgat ttttcattcc
     9481 gaaaaaagca gagcactaga aataagtaca tcaaatcaca atatggcatt aggcttgaag
     9541 attaaagaaa ctaaaaatgg gggggatttt gtcattcagc tttatgatcc caaccataca
     9601 gcaactcatt tacgagcaga gtttaacaaa tttaacttag ctaaaataaa aaaactgact
     9661 gtagataatt ttcttgatga aaaacatcag aaatgttatg gtcttatatc cgacggtatg
     9721 tctatatttg tggacagaca tactccaaca agcatgtcct ccataatcag atggcctaat
     9781 aatttacttc accccaaagt tatttatcac gcgatgcgta tgggattgac tgagctaatc
     9841 caaaaagtaa caagagtcgt acaactatct gacctttcag acaatacgtt agaattactt
     9901 ttggcagcca aaaatgacga tggtttgtca ggattgcttt tagctttaca aaatgggcat
     9961 tcagatacaa tcttagcata cggagaactc ctggaaactt ctggacttaa ccttgataaa
    10021 acggtagaac tactaactgc ggaaggaatg ggaggacgaa tatcgggttt atcccaagca
    10081 cttcaaaatg ggcatgcaga aactatcaaa acatacggaa ggcttctcaa gaagagagca
    10141 ataaatatcg aatacaataa gctgaaaaat ttgctgaccg cttattatta tgatgaagta
    10201 cacagacaga tacccggact aatgtttgct cttcaaaatg gacatgcaga tgctatacgc
    10261 gcatacggtg agctcattct tagcccccct ctcctcaact cagaggatat tgtaaatttg
    10321 ctggcctcaa ggagatatga caatgttccc ggacttctgt tagcattgaa taatggacag
    10381 gctgatgcaa tcttagctta tggtgatatc ttgaatgagg caaaacttaa cttggataaa
    10441 aaagcagagc tgttagaagc gaaagattct aatggtttat ctggattgtt tgtagccttg
    10501 cataatggat gtgtagaaac aattattgct tatgggaaaa tacttcacac tgcagacctt
    10561 actccacatc aggcatcaaa attactggca gcagaaggcc caaatggggt atctggatta
    10621 attatagctt ttcaaaatag gaattttgag gcaataaaaa cttatatggg aataataaaa
    10681 aatgaaaata ttacacctga agaaatagca gaacacttgg acaaaaaaaa tggaagtgat
    10741 tttctagaaa ttatgaagaa tataaaaatc tgaatattat ctcgtggtga tacactgaat
    10801 ttgaggtaac cggctcattt aaaccgtctg gtctgttacc tccggttcca caaaaataat
    10861 gtccatcatt ttgaagtgga cactatc
//
LOCUS       DAJTYD010000131        10768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTYD010000131 DAJTYD010000000
VERSION     DAJTYD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.131"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..829
                     /locus_tag="OO585_003014"
     CDS             <1..829
                     /locus_tag="OO585_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6630522.1"
                     /translation="DFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLE
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKATAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(871..2311)
                     /gene="aldA"
                     /locus_tag="OO585_003015"
                     /pseudo
     CDS             complement(871..2311)
                     /gene="aldA"
                     /locus_tag="OO585_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2466..3308)
                     /locus_tag="OO585_003016"
     CDS             complement(2466..3308)
                     /locus_tag="OO585_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6630523.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3580..7482)
                     /gene="hrpA"
                     /locus_tag="OO585_003017"
     CDS             complement(3580..7482)
                     /gene="hrpA"
                     /locus_tag="OO585_003017"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6630524.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7683..8288
                     /gene="azoR"
                     /locus_tag="OO585_003018"
     CDS             7683..8288
                     /gene="azoR"
                     /locus_tag="OO585_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6630525.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8339..9655)
                     /locus_tag="OO585_003019"
     CDS             complement(8339..9655)
                     /locus_tag="OO585_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6630526.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9645..>10768)
                     /locus_tag="OO585_003020"
     CDS             complement(9645..>10768)
                     /locus_tag="OO585_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HCR6630527.1"
                     /translation="RDAAAITLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLP
                     GFYHDTLGEENRALAFEKMQSFISRLYANKSQKFDYQHEDCTGPSADQWRLLSGGPVL
                     LSPVDLAYRFMRKAMKLFGTHSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLID
                     KIYLNSVGWRGIRQRKTHLQILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEP
                     AVSDILLRDYSELNVAQGQEMIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSG
                     LYELFPENEQVKNSLAGLANAIEPVGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMR
                     VRSQGEMDSLVRDAGFDKCTQRIDEWGIFTVSMAVRRDN"
BASE COUNT         2658 a         2913 c         2579 g         2618 t
ORIGIN      
        1 tgattttacg gaagattcac ttatcgttga tgggaaaagt atcgcggttt acgccgaaaa
       61 agaggctaaa aatattccgt ggaaagcgaa aggtgcagaa atcattgtcg aatgtactgg
      121 cttttatacc tccgccgaga aatcgcaggc gcatcttgat gctggtgcga agaaggtgtt
      181 gatttccgcc cctgccggtg aaatgaaaac tatcgtttat aacgtcaatg acgacactct
      241 ggatggcaac gacaccattg tttccgtggc gtcatgcacc actaactgtc ttgcgccgat
      301 ggccaaagcc ttgcatgaca gtttcgggat agaagtcggc acgatgacga ccattcatgc
      361 ctatactggc acccagtcac tggtggatgg cccacgtggt aaagatttac gtgcttcacg
      421 cgcagcggca gaaaatatca ttccccacac tacgggggcg gcaaaagcca ttggtctgga
      481 gatcccggaa ctgagcggca aactgaaagg tcatgcgcaa cgcgtgccgg tgaaaacagg
      541 ttcggtcact gaactggtat cgattctcgg aaaaaaagcg actgccgaag aggtgaataa
      601 cgcacttaaa caagcaacca ccaataacga gtcatttggt tataccgatg aagaaatagt
      661 ctcttccgat atcattggca gccatttcgg ttcggtgttt gatgccacgc aaacggaaat
      721 taccgccgtg ggcgatttac aactggtgaa aacggtcgcc tggtacgata acgaatatgg
      781 cttcgtcacg cagcttattc gcaccctcga aaaattcgct aaactctgac gcgcacaggc
      841 ggaggaaaaa acctccgcct cttttactca ttacgactgt aaataaacca cctgggtctg
      901 cagatattca tgcaggccat gtttaccatc tgcgccgcca ataccggatt tacgccatcc
      961 ggcgtggaag ccttgcatag cttcgaactt ttcacggttg atgtaagttt caccaaactt
     1021 cagcccttta atggctttca tcgcgacgtt cagattttgg gtatagattt gatgaggtca
     1081 ggccgtaatc actgtcatta gccattgaga tagcctcttc cagcgtgtca aatgcgacca
     1141 ccggcagaac cgggccaaag gtttcctcat gcataatcga catttcctgg cgaacatcca
     1201 gcagcaatgt cggcggataa taatatcctt tcccctctac cgctttgcca cccaacgcca
     1261 ctctcgcccc ttcttctact gcgcgcgcca ctttttgctc aaccctttcc aacgccgcgg
     1321 cgttaatcaa cggccccatc gcaatgtcgt tgcgttcagc ggggttacca aattgaaccg
     1381 cctgcatcgc ttcacccagc cgattgacga actgatcata aatgcctttc tgtacataaa
     1441 cacgttctgc acagttacac acttgcccac tattaatgac gcgtgaatca acgatggctt
     1501 tgactgccag ttcaagatcg gcatcgtcca ttacgatagc tggtgcttta ccccccaatt
     1561 ccagacacac tttggtgatg tttttcgccg cagtcgccat gatcttctca cctgcagaga
     1621 cgctgcctgt catactgacc attgcgacct ttgggttacc cgccagttct tgcccaacgg
     1681 tttcaccacg ccccagtaca aggttaaaca cgccgcgcgg aaggcctatt tcatcgacga
     1741 ttttggcgaa tgcaatcgca ttgtttggcg taaattcact aggtttaatg acgatggtat
     1801 taccggtcaa aagagcggga gccattttgc gggcaatgag gaagaacggg aagttccacg
     1861 gcagaatgcc ggtagtcaca ccaagcgcac gtttaaacaa aagaatattt tctcctggac
     1921 gatcgctttg aataatctcg ccctcgtaac gccgtgccca ctccgccatg taatcgatat
     1981 agtcggcagt aaaagccact tcgacttcag ccagctgctg gatcttgccc ccttcttcaa
     2041 caatcagcgc actgatttca ctggcgcgtt cgcggatccc ggcggagatt ttgcgcaacc
     2101 aactggcgcg ttcaatagca ggcaacgctt cccattctgg ttgtgcacgt tctgctgcat
     2161 cgattgcctt acgggcatcc tcggcctgac catcgggtat gcgggaaatg acagcctctg
     2221 tagcagggtt taccacatca atccatgcgt ctccacgcca ggtaacaaac tgtccatcga
     2281 tatacatagg atgttgaacg ggtactgaca tgggcgactc ctgtgattta tatgtttgtt
     2341 cttggtaaat tcattgttaa ttaacatcaa tcatcataga gacggcatcg tgaaaggaca
     2401 attctgtgaa gggcttcata aaaacaagtg taaaaggaat aaaaggcaga aataaaagtc
     2461 aggagtcatg cccgtttgca aacaagtggt tcacaaacgg gcaatttttg acgtaacgag
     2521 cgactttcca tcgcctcgat gagcaccgca tcgtgtttaa gcgtttgcca tgcatggatg
     2581 acttctgccg gaaaatcaac gtgaacgata aaatctcgcc cgcggggacc gtagccatcg
     2641 ctatcatcgc gtaaacgcgg cagctcgcca gtgagtagtg agagataacg ctcaaccggc
     2701 cataatcctt gtaactgatt aataaagatt acactgtctg cgttatttcc taactgagga
     2761 acgaatccgg gataacttaa ccagcgtggt gcatcgggat tgtccccagt catacggcgg
     2821 aacgtcgcca tcgtgcgccg ctgcatggtg gggtcctgaa caacgatagc cgtatgaact
     2881 cgttctacgg cctgattaaa tagcgcgatg ctaaagcgtg cgttttcacc gcagtttgtt
     2941 gactggtctt caatccagat tttttcatgc ggaatgtgcc agaactgatg agcgatatcc
     3001 gccaggatgg tcgcttctgc tctgccagtg gtgcggatag tgttgtagtg cggatgctgt
     3061 gcgatggcgc tatacaaaaa agttgtcgag tgaccgatac caccactaat cagtaaagga
     3121 atttgctgat cgcgagcaat cttacatgcc gcgtcgatag tcggcataac cgcattgcct
     3181 gcaaggatca cgcaatcggc ctgatacggc acctcaccgg agaaatcatc ctgcgccagc
     3241 cactgtccga taacatttat ggcatctata gttgccggcg aaagcgtcgg aaacggggta
     3301 atgttcatgg ttcctcctta tcgttccctt aagaatactg cgtgagataa tagtgagaag
     3361 ctgagcactc tgaaaggagg aagtcgtatg tccttaaccg ccactccgca agcctgacat
     3421 atcttttaag gaaatgctgt agataaacat tattcatctt gcagcagaga attgtatttt
     3481 tctgaattcc tgaattttta cccttaccgt tttagaatta ataaaaaacc cggcgctaaa
     3541 tgcttaccgg gttttagatt tattaggcaa atagcagggt taaccgctaa tctgctccat
     3601 cgcctgcaaa atacgcttat ctgaaatcgg ataaggcgta ccaagttgtt gagcgaagta
     3661 actaacgcgc agctcttcta tcatccaacg gatctctttc acgtcttcat cctcacgacg
     3721 tgcaggcggc agtttgttga tccattgctg ccacgcctgc tggacgtttt cgactttcag
     3781 catctgtgca cgatcgcgat gtggatcaac cgccagtttt tccagtcgtt tttcaatcgc
     3841 ctgcaaatat cgcagcgtgt cgcccagccg tttgaagccg ttaccagtga caaaaccgcg
     3901 atataccaac ccgcccatct gcgctttaat gtcagaaagc cccagtgcca tggtcatatc
     3961 cacccgccct ttcagacgtt tgttgatatt gaacactgcc gtaagaattt gctcgacctg
     4021 cttcgcaata tctaccaccg tgtcgttcag ttcggcacgc actttctcat gcagcgcagc
     4081 aaagccttct tccgtccaga ccgggccacc attggcgtcg atcagttgat ccacaccgca
     4141 ggagatacag tcgtcgatca gctccagcac tttgccatac gggttgaagt acagtcccag
     4201 cttggctttg ttcggtaact tttcatgtaa atacttgatc ggcgagggaa tatttagcag
     4261 cagtagacgg cgaagaccat tccacattgc ctgtttttgc tccagcgggt tatcaaacag
     4321 tttgatcgcc acgctgtcgc gttcatccac cagcgccggc cacgccttca ctttatagtt
     4381 gccacgcttc tgttcgtaac tttccggcag ctgaccaaaa ctccagatat gtaagccgct
     4441 ctgctcgata ccgtcatccg ccaccgcgga cagcgtttcc tgcactttgc ctttcagcgc
     4501 atctttcaga tcttgtagcg agcgcccttc ttttagcttc ttgtttttgt catccaccac
     4561 gcggaaggtg attttcaggt gatcgggcac ctgatcccag tgccagtctt cgcggtcaac
     4621 ggtaacgccg gtcatccgcc gtaactcgcg ctcaaggctg tcgagcaacg gtaactccag
     4681 cggtgtgacg cggcctaaaa acgcttcggc gtagtttggt gcaggcacaa aattacggcg
     4741 taccggtttc ggcaacgatt taatcagagc aatcaccaat tcgcggcgca gaccggggat
     4801 ctgccattca aacccgcttt cctcaacctg gttaagtaac ggcagcggaa tatgtacggt
     4861 cacaccgtcc gcgtctgcgc ctggctcaaa ctgatagctc aaacgcagct tgagattgcc
     4921 ctgatgccag aagttcgggt aatccagctt gctgattttt tccgcccctt ctttgatcaa
     4981 catgctcttt tcaaagttga gcaaatcagg cgtttcgcgg ctgacttttt tccaccagct
     5041 atcgaagtga cgagcggaga ttacatcgtg gctgatgcgc tggtcgtaga actcaaacag
     5101 cgtttcgtca tcaaccagaa tatcgcggcg acgtgatttg tgttccagct cttccacttc
     5161 ggcccgcagt ttcaggtttt cacggaagaa tgcgtgacgc gtctgccagt caccttccac
     5221 cagcgcatgg cgaataaaga gttcacgaca taacgccgga tcgatctggc tgtagttgac
     5281 cttgcgcgcg gcaacaatcg gcaaaccata aacagtgact ttttccgttg ccatcaccgc
     5341 gccctgcgcc cgttcccagt gcggttcgct gtaggtacgt ttaatcaaat gctgagcgac
     5401 aggctccacc cattccgggt cgatacgcgc agcaatgcgc ccccacaggc ggctggtttc
     5461 taccagttcc gccaccatta cccatttcgg cggttttttg aataaaccag aaccggggaa
     5521 gatggagaaa cgcgcgttac gtgcgccggt atattcttgt ttatcggcat ctttcatgcc
     5581 gatatgggaa agtaaacccg tcagcaacgc aatgtgaatt tcgcgatact ccgccggttc
     5641 gctgttaacc ggaatgccaa gttctttcac cacctgacgc aactgggtgt agatatcctg
     5701 ccattcgcgc acgcgcagat agttgagata atcggtacga cacaggcgac ggaaggcgtt
     5761 tgaagaaagc gccttttgct gctcgccaag ataattccac agatttacaa acgcgaggaa
     5821 gtcggattct ttgtcgtgga agcgacgatg tttttcgtcc gatgcctgct gtttgtccat
     5881 cggccgctca cgcgggtcct gaatggagag cgcggacgtg ataatcatcg cctcacgcac
     5941 gcagccatgt ttctgcgctt ccagcaccat acgcgccaga cgaggatcga ccggcagttg
     6001 cgagagctgg cgaccgagcg gcgtcagttt ataggcgctg gcctgttcat ccgtggtgat
     6061 cgcgcccagt tcttcgagca gacgcacgcc atcctggata ttgcgtttat ccggtgcttc
     6121 gacaaacggg aacgcagcga tatcgcccag ccccagcgcg gtcatctgca aaataaccga
     6181 ggccaggttg gtacgcagaa tctccggatc ggtaaactcc gggcgcgaga ggaaatcgtc
     6241 ttcggaatag agacgaatac agatcccttc ggacacacga ccacaacggc ctttacgctg
     6301 attggcagac gcctgtgaaa tgggttcaat cggcaaacgc tgcactttgg tgcgatagct
     6361 gtagcggctg atacgcgccg taccggggtc gataacgtat ttaatccccg gtacagtcag
     6421 cgaggtttcc gcgacgttgg tcgccagcac aatgcgtcgc ccgctgtgcg actggaacac
     6481 gcggttctgt tcgctattcg aaagccgcgc ataaagcggc aagatttcgg tatggcgtaa
     6541 gttcagcttg ttcagcgcat cggcggtatc gcgaatttcc cgctcgccgc tcataaagat
     6601 cagaatgtcg ccagggcttt cctggctcag ttcgtctacg gcgtcaaaaa tcgcctgcaa
     6661 ctgatcgcgc tcggtgtcat cggcttcttc aacaatcggg cgatagcgca cttccaccgg
     6721 ataggtccga ccggagactt cgataatcgg cgcattatta aagtggcgag aaaagcgttc
     6781 cgggtcgata gtcgcggaag tgataatgat ttttaggtca ggacgccgcg gcagcaactc
     6841 tttcaaatag ccgagcagaa aatcgatatt caggctgcgt tcgtgtgctt cgtcaataat
     6901 gatggtgtcg tactgcatca gcaggcggtc ttgctggatc tccgccagca ggataccgtc
     6961 ggtcatcagc ttgaccatcg tgttatcact tacgtgatcg ctgaaacgca ctttgtaacc
     7021 gatgcaaccg cctggctccg ttttcagctc ttccgcaata cggttcgcca ccgttcttgc
     7081 cgccagacga cgcggctggg tatggccgat cagtccttta atcccgcgcc ccagctccat
     7141 acagattttc ggtagctggg tcgttttacc ggaaccggtt tccccggcga cgatcaccac
     7201 ctggtgatca cgaatcgctt cgagaatatc ctgttttttc tgactaactg gcaaattgtc
     7261 cggataagtg atttccggtc gtgccgcttc acgcagcagg actttccctg ccgcctggtc
     7321 aatctctttc gccatctcct ggaaaatggc ctgttgtgca tcaggatttt taaccttctt
     7381 aacaccatgc agacggcgag aaaaacgcag tctgtcgcgc agcatcaacg aatccagccg
     7441 ctgctgcaaa gcagtaaagg tcaatttttg ttgttctgtc ataacgttag tgggcagtag
     7501 tctgcctgat taattttctt ttaaatgata gatatagagt accacatcgc ggctttctat
     7561 gcgttgttca ataaattcga acataggctt cgatttattg cgctatctct gtggcaggat
     7621 tgtgaataaa gtgtcagcaa gcaacggggc atcgccaatt caaacatcta taaggaaaca
     7681 ccatgagcaa ggtattagtt cttaaatcca gcatcctggc agggtactct cagtctaatc
     7741 agttgtccga ttattttgtt gaacaatggc gcgaaaagca ctccgctgat gaaatcaccg
     7801 ttcgcgacct ggctgcaaat ccgattccgg tactggatgg cgaactggtt ggcgctctgc
     7861 gtccgagcga tgcgccgctg actccgcgtc agcaggaagc tctggcgctt tccgatgagc
     7921 tgattgccga gctgaaagcc cacgacgtta tcgttattgc ggcaccgatg tataacttca
     7981 acatctcaac tcagttgaaa aactattttg acctggttgc acgcgcaggc gttactttcc
     8041 gctataccga gaacggtccg gaaggtctgg taacgggtaa aaaagccatc gttattacca
     8101 gccgcggtgg gatccacaaa gatggaccaa cggacctggt gacgccgtat ctgtccacgt
     8161 tcctcggctt tatcggcatt accgatgtga aatttgtctt cgccgaaggg atcgcatacg
     8221 gtccggaaat ggcagcgaaa gcgcagtctg acgcgaaagc ggccatcgac agcattgttg
     8281 ctgcataacc ggacacgatc ttgatgtgga ctgtagttca gtccacatct caatccactt
     8341 acctgttttc ccataatttc agcatcgctt tgtgatcttc cttaagcaca atatgcgaac
     8401 gtctggctcg aatataacta atcgcttcat caacggtttt acagtgtccg taacataaca
     8461 accatgccgc cactaccagc gcactgcgcg ataatcccaa cgcgcaatgg accagaacgc
     8521 tgccttgctc ttcgcgtaat gtttccagca tcgccacggc ctgtcggagc tccccctctt
     8581 ccggaaccac cagatccagc atcggtacac agaaatagag tcgatctttt gtcgctcgtc
     8641 cccgagggaa ttcaaaggtg acgtccagaa ccgcattctg tgccggaata tgtcgtggaa
     8701 acgcccctaa ataaacacca gcagtaattt tgctcaccgg ctccaggcga cgacaaaacc
     8761 aacgcataga cagccacatc ccgatgcgcc agggcaatgt cagccagtaa acggcgggcg
     8821 ggagtttccc ctgactatct ttccctgttg ttatcgcgcc aagcccaccg tacccacggc
     8881 caataatgag tagcgataat actggccaac ataaccagac tggccaccat aactgaatca
     8941 tcaccattag ctccatcaac acaatgcacg agcccgcgcc tacgacatat ggcagtgcta
     9001 ttttgattcg acgttgatca ggtttctgat aattccaacg acgatcgacg ggcaccatcc
     9061 agtcaatcaa catacctacc gccagccctg tgatgacatc aataaaatga tgctgccagg
     9121 tcgtcagagt agaaatagcg atgagtaaaa accatccgcc acagacttta cgccacctct
     9181 cagccagatg ctgacgaaag tgacgccaaa gtagccagca gagaataata tgcagcgaag
     9241 gagactggtt ataaggcaga tcaaacagtt caagttgcga aaatggccat cccgtcaccc
     9301 cactcacttc aggacggata aaactaaact tcagcggata gagcaaaaaa ccgcagcagg
     9361 ccattaccgt tgccagaata agccggtgga caagtcggcg ctgttcgaat gtcgtgctac
     9421 aaacgaacag cgaaaatcca tataaaagat ccagactcca gtaaggaaca atagtccagg
     9481 gaagaaaagg gatcgccgtt tcccaaccga atacctgact ggggatatca tggctgttaa
     9541 ggtcctgaac cgcggtgaac tgattaagag atccataggt gaagaaaaaa aacggggcca
     9601 gcaacaataa ccagccagcg ccttgtagca atacgttccg acgttcagtt atcacgacgc
     9661 accgccatcg aaaccgtaaa aataccccac tcatcaatcc gttgtgtgca tttatcaaat
     9721 ccggcatcac gcacgagtga atccatctcc ccttgcgaac gcacgcgcat tacccacggt
     9781 ttaccatctt tatgactggt taacaccccg gcaatcatct ccagttgagg gtgccacggc
     9841 tgcccggtgt agatgagaat gccgaccggt tcgatggcat tggcaagacc tgcgagtgag
     9901 ttttttacct gctcattttc gggaaaaagc tcatacagtc cagagacaat tgccagcgta
     9961 ggccgcggag ttaacgcgct gagttcctcc gggttaaacg catcgccctg ttcaaaacgc
    10021 acccgcccag acattccccg ttgggcaatc atctcttgcc cctgtgcaac atttaactcg
    10081 ctgtaatcac gtaacaaaat atcgcttacg gcaggctcgt ttgccagcgc atccagtaca
    10141 tagcgcccat gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt ggcgtggaga
    10201 tcggcaacgg cttgtttaat cagtatttgt aaatgggttt tgcgctggcg aataccgcgc
    10261 cagccaacac tgttcaggta gattttgtcg attaagcgcc cgaatgcgtt actaccttgc
    10321 ggttgatttt gatagacata atccagcgaa ctgcctgaat caaagccggt gctcattccg
    10381 agatgcaggc ccgcagagtg cgtcccgaac aatttcatcg cctttcgcat aaagcgatac
    10441 gctaaatcaa ccggcgataa tagcacgggt ccaccagaaa gtagccgcca ttgatccgct
    10501 gatggtcctg tgcagtcttc atgctgataa tcaaattttt gcgatttgtt agcatataaa
    10561 cgactaataa agctttgcat tttttcaaat gccagcgccc tgttctcttc acccaacgtg
    10621 tcgtgataaa agcctggcag cagatgcagc tctttcagag ggctacgtaa tctctgataa
    10681 aaatcaatct gcggttggcg atgcaccaca tagtcatcgc ctgatatcag aagttgcgtg
    10741 gggagcgtaa tcgccgccgc atctctaa
//
LOCUS       DAJTYD010000132        10735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTYD010000132 DAJTYD010000000
VERSION     DAJTYD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.132"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            83..424
                     /locus_tag="OO585_003021"
     CDS             83..424
                     /locus_tag="OO585_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6630528.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(415..1341)
                     /locus_tag="OO585_003022"
     CDS             complement(415..1341)
                     /locus_tag="OO585_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6630529.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1431..2429
                     /locus_tag="OO585_003023"
     CDS             1431..2429
                     /locus_tag="OO585_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6630530.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2426..2644)
                     /locus_tag="OO585_003024"
     CDS             complement(2426..2644)
                     /locus_tag="OO585_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6630531.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="OO585_003025"
     CDS             complement(2646..4661)
                     /gene="ligA"
                     /locus_tag="OO585_003025"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6630532.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIVGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="OO585_003026"
     CDS             complement(4732..5718)
                     /gene="zipA"
                     /locus_tag="OO585_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6630533.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5948..6709
                     /gene="cysZ"
                     /locus_tag="OO585_003027"
     CDS             5948..6709
                     /gene="cysZ"
                     /locus_tag="OO585_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6630534.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6894..7865
                     /gene="cysK"
                     /locus_tag="OO585_003028"
     CDS             6894..7865
                     /gene="cysK"
                     /locus_tag="OO585_003028"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6630535.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8249..8506
                     /gene="ptsH"
                     /locus_tag="OO585_003029"
     CDS             8249..8506
                     /gene="ptsH"
                     /locus_tag="OO585_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6630536.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8551..10278
                     /gene="ptsI"
                     /locus_tag="OO585_003030"
     CDS             8551..10278
                     /gene="ptsI"
                     /locus_tag="OO585_003030"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6630537.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10319..>10735
                     /gene="crr"
                     /locus_tag="OO585_003031"
     CDS             10319..>10735
                     /gene="crr"
                     /locus_tag="OO585_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6630538.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPV"
BASE COUNT         2612 a         2772 c         2657 g         2694 t
ORIGIN      
        1 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
       61 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
      121 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
      181 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
      241 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
      301 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
      361 gtctctggat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
      421 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
      481 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
      541 ctggcgttca acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
      601 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
      661 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
      721 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
      781 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
      841 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
      901 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
      961 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
     1021 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
     1081 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
     1141 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
     1201 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
     1261 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
     1321 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
     1381 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
     1441 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
     1501 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
     1561 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
     1621 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
     1681 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
     1741 acttgtgcac tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
     1801 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt
     1861 caccattgct ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
     1921 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
     1981 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
     2041 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
     2101 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
     2161 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
     2221 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
     2281 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
     2341 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
     2401 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
     2461 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
     2521 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac
     2581 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
     2641 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
     2701 tcctgcgcct tcgccagttt agatcctgca gcttcaccca caatcaccag atcggttttc
     2761 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
     2821 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
     2881 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
     2941 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
     3001 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
     3061 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
     3121 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
     3181 tgtgccgatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
     3241 agatctgccg gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
     3301 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
     3361 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
     3421 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
     3481 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
     3541 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
     3601 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
     3661 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
     3721 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
     3781 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
     3841 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
     3901 ctgaccggca acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
     3961 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
     4021 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
     4081 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
     4141 acttcaccac gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg
     4201 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
     4261 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
     4321 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
     4381 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
     4441 cgtatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
     4501 gtaatcagtt ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
     4561 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
     4621 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
     4681 aaacccccga caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt
     4741 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
     4801 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
     4861 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
     4921 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
     4981 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
     5041 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
     5101 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
     5161 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
     5221 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
     5281 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg
     5341 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
     5401 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga
     5461 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
     5521 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
     5581 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
     5641 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
     5701 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
     5761 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
     5821 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
     5881 ttgcgcaatt acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat
     5941 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
     6001 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
     6061 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
     6121 cccgactctc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
     6181 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
     6241 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
     6301 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
     6361 cgaatggcaa aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
     6421 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
     6481 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
     6541 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
     6601 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
     6661 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
     6721 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
     6781 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
     6841 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
     6901 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
     6961 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
     7021 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
     7081 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
     7141 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
     7201 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
     7261 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
     7321 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
     7381 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
     7441 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
     7501 ttgagccaac cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg
     7561 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
     7621 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
     7681 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga
     7741 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
     7801 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
     7861 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
     7921 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
     7981 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
     8041 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
     8101 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa
     8161 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
     8221 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc
     8281 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
     8341 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
     8401 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
     8461 aaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
     8521 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
     8581 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
     8641 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
     8701 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
     8761 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
     8821 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
     8881 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
     8941 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
     9001 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
     9061 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
     9121 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
     9181 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
     9241 ccaaccaacg aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa
     9301 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
     9361 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
     9421 ggcgttggtc tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa
     9481 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
     9541 gttcgtacca tggacatcgg cggcgacaaa gaactgccat acatgaactt cccgaaagaa
     9601 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
     9661 cgcgatcaac tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
     9721 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
     9781 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
     9841 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
     9901 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
     9961 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    10021 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    10081 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    10141 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    10201 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    10261 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    10321 gggtttgttc gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat
    10381 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    10441 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    10501 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    10561 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    10621 cgaaggcttc aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat
    10681 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggtt
//
LOCUS       DAJTYD010000133        10679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTYD010000133 DAJTYD010000000
VERSION     DAJTYD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.133"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..309)
                     /locus_tag="OO585_003032"
     CDS             complement(<1..309)
                     /locus_tag="OO585_003032"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6630539.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     A"
     gene            complement(518..1177)
                     /locus_tag="OO585_003033"
     CDS             complement(518..1177)
                     /locus_tag="OO585_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6630540.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(1504..1860)
                     /locus_tag="OO585_003034"
     CDS             complement(1504..1860)
                     /locus_tag="OO585_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6630541.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(1927..2221)
                     /gene="yebG"
                     /locus_tag="OO585_003035"
                     /pseudo
     CDS             complement(1927..2221)
                     /gene="yebG"
                     /locus_tag="OO585_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            2355..3533
                     /gene="purT"
                     /locus_tag="OO585_003036"
     CDS             2355..3533
                     /gene="purT"
                     /locus_tag="OO585_003036"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6630542.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(3589..4230)
                     /gene="kdgA"
                     /locus_tag="OO585_003037"
                     /gene_synonym="eda"
     CDS             complement(3589..4230)
                     /gene="kdgA"
                     /locus_tag="OO585_003037"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6630543.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4267..6078)
                     /gene="edd"
                     /locus_tag="OO585_003038"
     CDS             complement(4267..6078)
                     /gene="edd"
                     /locus_tag="OO585_003038"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6630544.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRRFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6313..7788)
                     /gene="zwf"
                     /locus_tag="OO585_003039"
     CDS             complement(6313..7788)
                     /gene="zwf"
                     /locus_tag="OO585_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6630545.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8125..8994
                     /gene="hexR"
                     /locus_tag="OO585_003040"
                     /gene_synonym="yebK"
     CDS             8125..8994
                     /gene="hexR"
                     /locus_tag="OO585_003040"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6630546.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9122..10564
                     /gene="pyk"
                     /locus_tag="OO585_003041"
     CDS             9122..10564
                     /gene="pyk"
                     /locus_tag="OO585_003041"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6630547.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
BASE COUNT         2572 a         2756 c         2685 g         2666 t
ORIGIN      
        1 tgccgattga cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg
       61 gtagccattt ttgatgtagg gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc
      121 ctttaagatg cgatcctgca aggcttgttg tgaggccatc acccgatgac cgtaactatt
      181 ttcttgttgc aggtagtcca ggacttctgg ctgagaacgc gtatcgtccc gcagccagta
      241 gtaattatcg atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt
      301 tggtagcatg ttattgttct ttctggttga aacatcttat aagggtggca aaactcaccg
      361 gggatgcaag cgaaacaggg gagtcattgc ttagatgatg acaggtaatg gcgcggatat
      421 cgaatgttat gcagacagag aaaatcagcc tgttcaaatg gctgtgcgat tctggatagc
      481 ccgaaatagt caacttcagg ctatccagag agcggaatta ttccgccaaa gtgcgttttt
      541 gctgttcgag atcgcgttca atgccgtcac gaacatcctg ggggattttc agcgcgtcac
      601 ccagtgcatt caggtaactg cgttccataa aatggtcaat atcaatagcc gcgcaactca
      661 ggaaatagat ttccagcgcc tcttcttcat tgcggacccc ggtagccaag cgttgtggat
      721 ccagcggttg ttcgattgcc tgctcaatga gtacacgccc ctgctcttcc acgccggctt
      781 cacgcaattg ctggtcgatt gccgcacgtt ctttggcatc aatatgacca tcacttttag
      841 cggcaaagac cagcgcaagg atcaaacgtt ctgtacgctc atccagcggc gtactttgcg
      901 cgccaaactg cggttcgtcc tgatgcgccg cgcgaatttt atctttgtat ttattccaca
      961 gcaccgtacc cgctaccgct ccgccgccaa ccagtaacgc gttggtgcca tatttcgtaa
     1021 gaagtttacg tgctgattta tttgcgacca gcagcccagc cagtccgcct aatgcgcctg
     1081 gcactaacag tttgcccaat ccctgatccg cagacgagga gatagaagaa ctgctttgcc
     1141 caagcaggga ttgcagttga tttaaccagt tagccatatt tgctcctcaa taaccatttt
     1201 tagtgcctgt agcatagcgg atgaggatgt cagaaaatgt atatcgagac aaaagatgcg
     1261 caaataagcg gggcggtagg ccgttattca aagaaattcg cgccaggtaa ggtatttact
     1321 gtatatcagg tgataagggt attttaggtg aaagtagttt gtcgttttga gtggcattgt
     1381 ttgatgctga ttgcccgatg cgacgctgtt gcgtcttctc aggcctacga ttgtggtgcg
     1441 ttctggggcg gacaaggcac tcgcgccgca tccgccaacg gtcaatcgaa ttatcagaag
     1501 ccattaacgc cgctgatatt ccgccattcc cgctttgcag tccacgctga cctggtaatg
     1561 aatatcggca cttttaccac gcacggttag cggtactgac catttatcat ctttaccctg
     1621 aatgtcctgc aaactgaccc aagccacggg atcggcctga ccgacaattt tttgatcatc
     1681 tgcccaacgc gccacgcgat tttgttgata atcacgtttt acgctcgcgg caattccggc
     1741 agcatccaga ccttcacact ttgggaaagt gaccgacttg ctggtttcat tattggcagc
     1801 gaaaactgat gcgcaggcag aaaccaacaa cagccctaaa aacgcccctc tttttttcat
     1861 gtttttctcc atagcacaat gattcaggag aaagcatggt acaaattgtc aggagcgcaa
     1921 gttgcttcag gccgcgtgtg aggcatcttc ctcttcggat tcagcaccga cgacctgtgg
     1981 gtggggacgg taatttgccg gttttcagaa tggtgctcag cacatctttt tgttctgcca
     2041 gccatagcga aagggcttca cgttgctcgt cttccatttg cactggagaa ttagtcagcc
     2101 aggtgtcaag cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc
     2161 tggtaaacga cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca
     2221 tttgcagcct ctcataataa ctgtgatttt atacagtgta tttcttttcg gttgagaaat
     2281 caaaatcaac aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc
     2341 aggggatatt cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga
     2401 tgttattagg ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg
     2461 tagaggtgat tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct
     2521 cccatgtcat taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac
     2581 cacattatat cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag
     2641 aggaaggact gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg
     2701 gtatccgtcg cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg
     2761 atagcgaaag ccttttccgc gaggcggttg ctgacattgg ctatccctgc attgtaaaac
     2821 cggtgatgag ctcttccggc aaggggcaga cgtttattcg ttctgcagag caacttgctc
     2881 aggcatggaa gtacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag
     2941 gcgtcgttaa gtttgacttc gaaattacct tgctaaccgt cagcgcggtg gatggcgtcc
     3001 atttctgtgc accggtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac
     3061 cacagcaaat gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc
     3121 tggcactggg cggttatggt ctgtttggtg tcgagctgtt tgtctgtagt gatgaggtga
     3181 ttttcagtga ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag
     3241 atctttcaga gtttgccctg catgtacgtg ccttcctcgg acttccggtt ggcgggatcc
     3301 gtcagtatgg tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca
     3361 cgtttgataa tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc
     3421 cggaaattga tggcagccgt cgtctgggtg ttgcgctggc gaccgctgag agtgttgttg
     3481 acgccattga acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc
     3541 aaaaaaacgc tacaaaaatg cccgatcgta gatcgggcgt tttgactttt acagcttagc
     3601 gccttctaca gcttcacgcg ccagcttagt aatgcggtcg taatcgcccg cttccagcgc
     3661 atctgccgga accagccagg aaccaccgat gcacagcacg cttttcagcg ccaggtagtc
     3721 acggtagtta gccggagaaa taccacccgt cgggcagaaa cggacctggg agaacggacc
     3781 cgcgatcgcc tgcagggctt tcacgccgcc gttagcttca gccgggaaga atttgaactc
     3841 tttcaaaccg tagtccatac ccagcatcag ttcggaaaca gtgctgatcc ccggaattag
     3901 aggaatagtc ccttcggtag cagctttcag cagcggctcg gtcagacccg ggctaattgc
     3961 gaactgtgca cccgcttcag tgacttctgc cagctgctgt ggattcagca ccgtaccggc
     4021 acccacaatc gcttcaggca cttctttggc gatagcacgg atagcgtcaa ctgcacactc
     4081 ggtacgcaga gtcacttcca gaacgcgcac cccaccagca accaacgctt ttgccatcgg
     4141 cactgcgtgt tccagttttt ttaccacgat aaccggtaca accgggccgg tggtcaggat
     4201 tgattctgca cttgttttcc agtttttcat cagagttttc tctcgcctga ttacaaattt
     4261 gtcgtcttaa aaagtgatac aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa
     4321 ggcgctgaat aattcacgtc ctgtccccac gcgtgacgcg ctcaggtcag gaatgtgcgg
     4381 ttcgcgagca gccagttccg cttcgtctac cagcagcgtc agttcgcctg tctgtccatt
     4441 cacacgaatg atgtccccgt cgcgcacttt tgccagcagc ccgccatcgt aggcttctgg
     4501 tgttacgtgg atagctgacg gcactttacc tgaagcgccg gagagtcgtc catcggtaac
     4561 taacgcaatt ttgaaacgcc ggtccaataa tacaccaagt ggcggcatga gtttatgtaa
     4621 ttctggcatt ccgttcgctt ttggcccctg atgacggaca acaacgacac aatcgcggtc
     4681 cagcaaaccc gcttcaaagg ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg
     4741 cgcttcaatc acctggttct caaccggcac ggcagaggtt ttcataaccg cacggcccag
     4801 gttaccgctt aacacttttg tcccaccatg atgagagaaa ggttgttcga aggaagcgat
     4861 cacattgctg tcgagtgatt tttccgcccc ttcccgccag tccagttcac cattattcag
     4921 ccatggttca agggtataac gagacagacc aaagcctgcc accgtattga catcttcatg
     4981 cagcaggcct gctttgagca gttcacgcac cagaaccggt acgccacctg ccgcctggaa
     5041 gtggttaata tcggccggac cgttcgggta gagacgtgcc atcagcggta caacatcaga
     5101 aaggtcagag aagtcatccc agttaatctg aataccggcc gcgcgcgcca tcgccaccag
     5161 gtgcatggtg tggttagtgg aaccaccggt cgccagcagt gcaacgatac cgttcaccac
     5221 cactttctca tcgatcatct taccgatcgg catccattca ttaccattac cggtcatgcg
     5281 tgtaacctga cgcgcagcgg cagcggtcaa agcatcgcgc agcggagaat ccggatgaac
     5341 aaaagaagag cctggcgact gcatccccat aaattccacc accatctggt tggtgttggc
     5401 agtaccgtag aaagtacatg ttcccggcgc atggtaagac gcggcttctg actccagtaa
     5461 ggccatgcgg tccactttac cttcggcata aagctggcga atacgcactt tttctttatt
     5521 tggcaaaccg cttgccatcg gtccagacgg cacaaacacc gcaggcaaat gaccaaacga
     5581 cagggctgcc atcgtcagac ccgggacaat cttgtcgcac acaccgagga acagagcacc
     5641 atcaaacatg ttatgggaca gccccaccgc cgcagacatc gctatcactt cgcggcttag
     5701 cagcgacaat tccattccat cctgcccctg ggtgacacca tcacacatcg ccggaacacc
     5761 gcccgcaacc tgaccaaccg cattcgcttc atgcagggct ttacgaatga tttctggata
     5821 gtgttcataa ggctggtgcg cggagagcat gtcgttatag gaggtgatga tggcgatatt
     5881 gttacgcaac atgcttttca aagaggcttt gtcttctggc tggcaggcag cgaaaccgtg
     5941 tgccaggtta ccgcatgcca actgcgaacg atgaacggtc gacgttttcg cttgttctat
     6001 ccgggcgaga taagcagagc gagtctcgcg cgaacgttca atgattcgat ttgttacgcg
     6061 taacaattgt ggattcataa aggctcctga aattgagttg tcagagcagg atgattcaca
     6121 acgcgtttca ttcagaggat ttatgagtga aacgcctgta accggagctc atagggcaaa
     6181 aacgtttcag tcagtgtaat aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag
     6241 tgtaaaaatt gttctacaat ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga
     6301 taagcgcaga tattactcaa actcattcca ggaacgacca tcacgggtaa tcatcgccac
     6361 cgaggcaacg ggtccccagg ttccggcctg atacggtttc ggcgcatcat tgtccatcgc
     6421 ccacgcctca gtaatggagt ctacccattt ccaggcttct tccacttcat cacgacgtac
     6481 aaacagtgcc tgaataccac gcatggtttc cagcagcaga cgttcatagg catccgccag
     6541 atgcgtctga ttaaaggttt ctgaatagct cagatccagc ttggtgattt gcaggttatg
     6601 tttgtggtca aggccaggaa ctttattcag tacctggata tccacgcctt catcaggttg
     6661 cagacggata gtcagtttat tctgcggcag atcctgccac gactctttaa acagattcag
     6721 ttcaggtgtt ttgaaataga ccacgacttc agaacatttg gtcggcagac gtttaccagt
     6781 acgcaggtag aatggcacac cggcccagcg ccagttatca atgtcgacgc ggatcgccac
     6841 gaaggtttct gtattgctgc tcttgttcgc accctcttct tccagatatc ccggcacttt
     6901 tttgccctgg gcgaagcccg cagtatattg cccacgtacg gttttttcgc gtacgttgga
     6961 gcggtcgatg cggcgcaggg acttcagtac tttcactttt tcatcgcgga tgctgtctgc
     7021 gctcaggtca gatggcggag acatcgcaat catgcagaga atttgtagca ggtggttctg
     7081 aatcatgtca cgcatctgac cggctttatc aaaatagccc cagcgccctt cgatccccac
     7141 ttcttctgcc acggtaatct caacatgatc aatggtgcga ttgtcccagt tattcacaaa
     7201 cagggagtta gcaaaacgca gcgccaacag gttcagcacc gtttctttac caagatagtg
     7261 gtcgatacgg taaacctggc actcctcgaa gtattcgcca acctgatcat tgatttcctg
     7321 cgaggtcgcc agcgacgtcc ccagcggttt ctccatgact acgcgtgccg gtttagcatt
     7381 cagttttgcc tcgccaagcc ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa
     7441 gtagttaatg gtgatacgat ttttttgatc cagcatcgcg ccgagacggt tgaatgcagc
     7501 agtgtcattg acatcgagat tacaaaaatc cagacgtgca ctcagggtgt cccataaacc
     7561 ttcatcaatg gtttctttca tgaaagtttc gagcgcctcg cggacaactt tggtatatgc
     7621 cgctttatcc cagtcagcac gccctacgcc gataatccgg gtgtccgggt tgagctgacc
     7681 ggctttttcc agttgataca gggaaggcag caatttacga cgcgcaaggt cgcctttcgc
     7741 gccgaaaatg accaggtcac aggcctgggc tgtttgcgtt accgccatgt cattctcctt
     7801 aagttaacta acccggtact taagccaggg tatacttgta attttcttac ggtgcactgt
     7861 actgctttta cgagcttgcg aaaactgtaa acgcttatcc acccgtgcga ttacgggaaa
     7921 agcgcgcaaa gcgcggcaaa actgataaaa aatcatcgtt ttccattcgg taaaaatctg
     7981 acactgatca tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg
     8041 gcacacaggc gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc
     8101 attacccgtg agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa
     8161 catttgagca aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg
     8221 atccattcga gtattgctgc tatggcactg gaagccaatg ttagcgaacc gacggtgaat
     8281 cgtttctgtc gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacag
     8341 agtctggcga atggcactcc ctatgttaat cgcaatgtca atgaagatga cagcgttgaa
     8401 tcatacacag ggaaaatatt tgagtccgca atggcaacgc ttgatcatgt ccgtcattca
     8461 ctggataaat ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc
     8521 tttttcggat taggctcttc agccgccgtt gcccacgatg cgatgaataa gttctttcgt
     8581 tttaatgttc cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat
     8641 tgtagcgacg gagacgtggt ggtgctgatt tctcacactg gaagaacaaa aaatctggtc
     8701 gagctggcgc agctggctcg cgaaaacgac gcaatggtga ttgccctcac ctctgcgggt
     8761 accccgctcg cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac
     8821 atttatatgc ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca
     8881 ggatttactt tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg
     8941 ctgaaggaat cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaaacga
     9001 ctgtcactgt cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg
     9061 ttatttcatt cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta
     9121 catgtccaga aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg
     9181 cgataataat cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc
     9241 tcacggctcg cctgaagatc acaaaatgcg cgcggataaa gttcgtgaga ttgccgcaaa
     9301 actggggcgt catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac
     9361 ctttaaagaa ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct
     9421 gggtaaaggt gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga
     9481 cgtcgtgcct ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga
     9541 agttcagggc atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa
     9601 aggtatcaac aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc
     9661 agacattaag actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg
     9721 tggcgaagat ctgaactatg cccgtcgctt ggcacgcgat gcaggatgtg atgcgaaaat
     9781 tgttgccaag gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat
     9841 cctcgcctct gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc
     9901 ggaactggtc ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt
     9961 aatcacggcg acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga
    10021 agtcatggac gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga
    10081 aactgccgca gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg
    10141 tgcggaaaaa atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa
    10201 tgtggaagaa gctattgcca tgtcagcaat gtacgcagct aaccacctga aaggcgttac
    10261 ggcgattatt accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc
    10321 tggtctacca attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta
    10381 tcgtggcgtt acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga
    10441 agcggttaat ctgctgcgcg ataaaggtta cttgatgtct ggtgacctgg tgattgtcac
    10501 ccagggcgac gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga
    10561 gtaatctcac ctgccccggc cgctgatggc cggggtattt agcaacacat aaattaggac
    10621 tacttattca atccaatcat aatcacagcc ccctgtaaaa tgaatacagg gatagcctg
//
LOCUS       DAJTYD010000134        10645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTYD010000134 DAJTYD010000000
VERSION     DAJTYD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.134"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..456
                     /locus_tag="OO585_003042"
     CDS             <1..456
                     /locus_tag="OO585_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6630548.1"
                     /translation="DMPMADFRCAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMA
                     AENKNINMTSYASSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIE
                     QKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            685..1275
                     /gene="uidR"
                     /locus_tag="OO585_003043"
     CDS             685..1275
                     /gene="uidR"
                     /locus_tag="OO585_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6630549.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1663..3474
                     /gene="uidA"
                     /locus_tag="OO585_003044"
     CDS             1663..3474
                     /gene="uidA"
                     /locus_tag="OO585_003044"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6630550.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3471..4844
                     /gene="uidB"
                     /locus_tag="OO585_003045"
     CDS             3471..4844
                     /gene="uidB"
                     /locus_tag="OO585_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6630551.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            4883..6148
                     /gene="uidC"
                     /locus_tag="OO585_003046"
     CDS             4883..6148
                     /gene="uidC"
                     /locus_tag="OO585_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6630552.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6374..7882)
                     /locus_tag="OO585_003047"
     CDS             complement(6374..7882)
                     /locus_tag="OO585_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6630553.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7983..9158)
                     /gene="manA"
                     /locus_tag="OO585_003048"
     CDS             complement(7983..9158)
                     /gene="manA"
                     /locus_tag="OO585_003048"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6630554.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9357..>10645
                     /gene="fumB"
                     /locus_tag="OO585_003049"
     CDS             9357..>10645
                     /gene="fumB"
                     /locus_tag="OO585_003049"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6630555.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEG"
BASE COUNT         2653 a         2493 c         2734 g         2765 t
ORIGIN      
        1 gatatgccaa tggcggattt tcgctgtgct tatgaactga atgtgttttc ttttttccat
       61 ctgtcacaac ttgttgcgcc agaaatggaa aaaaatggcg gtggcgttat tctgaccatc
      121 acttctatgg cggcagaaaa taaaaatata aacatgactt cctatgcatc atctaaagct
      181 gcggccagtc atctggtcag aaatatggca tttgacctgg gtgaaaaaaa tattcgggtg
      241 aatggcattg cgccgggggc aatattaacc gatgccctga aatccgttat tacaccagaa
      301 attgaacaaa aaatgttaca gcacacgccg atcagacgtc tgggtcaacc gcaagatatt
      361 gctaacgcgg cgctgttcct ttgctcgcct gcagccagct gggtaagcgg acaaattctc
      421 accgtctccg gtggtggggt acaggagctc aattaataca ctaacgaacc ggtaaacagc
      481 cgtatgtgtt gtttaccggg atagcgtgtg caattcatca acgtctctgc taaacaactg
      541 gcagccaaat cacggctatt ggttaaccaa tagctggatg aaaagcatac gaatagagtg
      601 tgccttcgca ctattcaaca gcaatgatag gcgctcacct gacaacgcgg taaactagtt
      661 attcacgcta actataatgg tttaatgatg gataacatgc agactgaagc acaaccgaca
      721 cggacccgga tcctcaatgc tgccagagag attttttcag aaaatggatt tcacagtgcc
      781 tcgatgaaag ccatctgtaa atcctgcgcc attagtcccg ggacgcttta tcaccatttc
      841 atctccaaag aagccttgat tcaggcgatt atcttacagg accaggagag ggcgctggcc
      901 cgtttccggg aaccgattga agggattcat tttgttgact atatggtcga gtctattgtc
      961 tctctcaccc atgaagcctt tgggcaacgg gcgctggtgg tcgaaattat ggcggaaggg
     1021 atgcgtaacc cacaggtcgc cgccatgctt aaaaataagc atatgacgat cactgaattt
     1081 gttgcccagc ggatgcgtga tgcccagcaa aaaggcgaga taagcccaga catcaacacg
     1141 tcaatgactt cacgtttact gctggatctg acctacggtg tactggccga tatcgaagcg
     1201 gaagatctgg cgcgtgaagc gtcgtttgct cagggattac gcgcgatgat tggcggtatc
     1261 ttaaccgcat cctgatcctc tcatttcgcg ggctggtgat aactgtgccc gcgtttcata
     1321 ctttaatttc cctgtacaaa atacaatcta gccgggatcg gagaattccc ccccaaaata
     1381 ttcaccgtag ccatatgtca tgagagttta tcgttctcaa gatgcccgaa cgaacgttcg
     1441 attgcttatt ttgtggcttc tgtaaacgct gttttaaaga ttaatgcgat ctatatcacg
     1501 ctgtgcgtat tgcagatttt ggttttttga tcgcggtgtc agttcttttt atttccattt
     1561 ctcttccatg ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga
     1621 ttaaaggttg accagtattt ttatttgaat gaggagtccc ttatgttacg tcctgtagaa
     1681 accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa
     1741 aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct
     1801 gtgccaggca gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac
     1861 gtctggtatc agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg
     1921 cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag
     1981 catcagggcg gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa
     2041 agtgtacgta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     2101 atggtgatta ccgatgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     2161 tatgccggga tccatcgcag cgtaatggtc tacaccacgc cgaacacctg ggtggacgat
     2221 atcaccgtgg tgacgcatgt tgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     2281 gtagcaaatg gtgatgtcag cgttgaactg cgtgatgcgg atcagcaggt ggttgcaact
     2341 ggacaaggta ccagcgggac tttgcaagtg gtgaatcccc acctctggca accgggtgaa
     2401 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     2461 ctgcgcgtcg gcatccggtc agtggcagtg aaggacgaac agttcctgat taaccacaaa
     2521 ccgttctact ttactggctt tggtcgtcat gaagatgcgg atttgcgagg caaaggattc
     2581 gataacgtgc tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac
     2641 cgaacctcgc attaccctta cgctgaagag atgctcgagt gggcagatga acatggcatc
     2701 gtggtgattg atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa
     2761 gcgggcaaca agccgaaaga actgtacagc gaagaggctg tcaacgggga aactcagcag
     2821 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     2881 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacgaga atatttcgcg
     2941 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     3001 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     3061 cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     3121 aaagaacttc tagcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     3181 gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     3241 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     3301 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     3361 aagaagggga tcttcacccg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa
     3421 cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgaatcaac
     3481 aactctcctg gcgcaccatc gtcggctaca gcctcggtga cgtcgccaat aacttcgcct
     3541 tcgcaatggg ggcgctcttc ctgttgagtt actacaccga cgtcgctggc gtcggtgccg
     3601 ctgcggcggg caccatgctg ttattggtgc gggtattcga tgccttcgcc gacgtctttg
     3661 ccggacgagt ggtggacagt gtgaataccc gctggggaaa attccgcccg tttttactct
     3721 tcggtactgc gccgttaatg atcttcagcg tgctggtatt ctgggtaccg accgactgga
     3781 gccatagcag caaagtagtg tatgcatatt tgacctacat gggcctcggg ctttgctata
     3841 gcctggtgaa tattccttat ggttcacttg ctaccgcgat gacccaacaa ccacaatccc
     3901 gcgcccgtct gggcgcggct cgtgggattg ccgcttcatt gacctttgtc tgcctggcat
     3961 ttctgatagg gccgagcatt aagaactcca gcccggaaga gatggtgtcg gtataccatt
     4021 tctggacgat tgtgctggcg attgccggaa tggtgcttta cttcatctgc ttcaaatcga
     4081 cgcgtgagaa tgtggtacgt atcgttgcgc agccgtcatt gaagatcagt ctgcaaaccc
     4141 tgaaacggaa tcgcccgctg tttatgttgt gcatcggtgc gctgtgtgtg ctgatttcga
     4201 cctttgcggt cagcgcctcg tcgttgttct acgagcgcta tgtgttaaat gataccgggc
     4261 tgttcactgt gctggtactg gtgcaaaact tggttggtac tgtggcatcg gcaccgctgg
     4321 tgccggggat ggtcgcgagg agcggtaaaa agaatacctt cctgattggc gctttgctgg
     4381 gaacctgcgg ttatctgcta ttcttctggg tttccgtctg gtcactgccg gtggcgttgg
     4441 ttgcgttggc catcgcttca attggtcagg gcgttactat gactgtgatg tgggcactgg
     4501 aagctgatac cgtagaatac ggtgaatacc tgaccggcgt gcgaattgaa gggctcacct
     4561 attcactatt ctcatttacc cgtaaatgcg gtcaggcaat cggaggttca attcctgcct
     4621 ttattttggg attaagcgga tatatcgcca atcaggtgca aacgccggaa gttattatgg
     4681 gcatccgcac atcaattgcc ttagtacctt gcggatttat gctactggca ttcgttatta
     4741 tctggtttta tccgctcacg gataaaaaat tcaaagaaat cgtggttgaa attgataatc
     4801 gtaaaaaagt gcagcagcaa ttaatcagcg atatcactaa ttaatattca ataaaaataa
     4861 tcagaacatc aaaggtgcaa ctatgagaaa aatagtggcc atggccgtta tttgcctgac
     4921 ggctgcctct ggccttacct ctgcttatgc ggcgcaactg gctgacgatg aagcgggact
     4981 acgcatcaga ctgaaaaacg aattgcgcag ggcggataag cccagtgctg gcgcgggaag
     5041 agatatttac gcatgggtac agagaggatt gttcgatttc aatagtggtt attattccaa
     5101 tattattggc gttgaaggcg gggcatatta tgtttataaa ttaggtgctc gtgctgatat
     5161 gagtacccgg tggtatcttg atggtgataa aagttttggc tttgccctgg gggcagtaaa
     5221 aataaaaccc tgtgaaaata gcctgcttaa attaggtcgc ttcgggacgg attatagtta
     5281 tggtagctta ccttatcgta ttccgttaat ggctggcagt tcgcaacgta cattaccgac
     5341 agtttctgaa ggagcattag gttattgggc tttaacacca aatattgatc tgtggggaat
     5401 gtggcgttca cgagtatttt tatggactga ttcaacaacc ggtattcgtg atgaaggggt
     5461 gtataacagc cagacgggaa aatacgataa acatcgcgca cgttcttttt tagccgccag
     5521 ttggcatgat gataccagtc gctattctct gggggcatcg gtacagaaag atgtttccaa
     5581 tcagatacaa agtattctcg agaaaagcat accgctcgac ccgaattata cgttgaaagg
     5641 ggaattgctc ggcttttacg cgcagctcga aggtttaagt cgtaatacca gccagcccaa
     5701 tgaaacggcg ttggttagtg gacaattgac ctggaatgcg ccgtggggaa gcgtatttgg
     5761 cagtggtggt tatttgcggc atgcaatgaa tggtgccgtg gtggataccg acattggcta
     5821 tcccttttca ttaagtcttg atcgtaaccg tgaaggaatg cagtcctggc aattgggcgt
     5881 caactatcgt ttaacgccgc aatttacact gacatttgca ccgattgtga ctcgcggcta
     5941 tgaatccagt aaacgagatg tgcggattga aggcacgggt atcttaggtg gtatgaacta
     6001 tcgggtcagc gaagggccgt tacaagggat gaatttcttt cttgctgccg ataaagggcg
     6061 ggaaaagcgc gatggcagta cgctgggcga tcgcctgaat tactgggatg tgaaaatgag
     6121 tattcagtat gactttatgc tgaagtaaga aataacgccg gagagaaaaa cctccggcgt
     6181 ttcagattgt tgacaaagtg cgcgtttttt atgccggatg cggcgtaaac gccttatcca
     6241 gcctacaaaa actcataaat tcaaagtatt gcaggaaaag gtaggcctga taagcgtagc
     6301 gcatcaggca atctctggtt tgttttcaga tgaaaacgcc gaagtgaaaa ttctccggcg
     6361 ttttggccgt gaattactgc tgcggaattg ccggtacagc cggaacgtta agagctggca
     6421 tcgcaaacat gccaacaaaa tcttctaacg acattttctg cccatttaac gttatctgac
     6481 cgttagtata ttgcaggctg gtggtgatgg tattgtcctg caaggtggtc agacggaaca
     6541 tctgccccat tgctgatgca ccttcaactt gctgtttcgc cagttttttc gcttgatctt
     6601 cctgataacc ttccagcttc gctacctgag tcataaactc agttgccata tccaccggaa
     6661 tggtcagttt cgcatccaga gatttaaccg aacgatctac ttcctgcgcc agcgtttgcg
     6721 gcgcttcttt agtcgttgcc ggatctttca ggaacagcga cagattcagc gcactttcac
     6781 cctgactgtt tttccagctt agcggcgcga cagtaatcac cggatcgcct ttcaacatca
     6841 gcggcagggc gctaaagaag gcttccgtca ctttctcctg ataaagttcg ggattgttgg
     6901 caatttctgg ctgtgccagc agcgcctgag tttgcgcgtt atattgctgg ctgaactgat
     6961 gccaggcttc accatcaatc tggccgactt ttaaagtcag cttgccgctg cccagatcct
     7021 gattctgtac cttcaggctg tttagcgagt aatccagttg gctattgatc gttttaccgt
     7081 cattgaccag atccgattta ccgctgatct ccatgccttc cagcagtgcc agttctttgc
     7141 cttccactga aatggtcatt ttttccagtg acagtttttg gtttcctacg cgctcaccaa
     7201 aacttgccag cgtgctggaa ccgtcggttt tcagattatt aaaggtcaac tgcactttct
     7261 ggttgtattc gttaactgcg tctatccgac cactttgcgc ctccccggaa agggagatgg
     7321 ctttgccgtc tctgtcagca tttaactgga actcgccgcc gctaaaggcg actttttcat
     7381 ccttttgctc gtaattcagt ggcttgagcg aaatatcgga actggaatca ccgctgtaac
     7441 caatgcgcga gttaatctca aaaggcgttt caccttttgc catatcaaac agtggtttgc
     7501 ttacttcgtt attaaccagc gtggtttgaa ttgatgccat cgacgggatc aggttcagtt
     7561 ttttaagctg ggcaagcggg aagggaccat gatcaaccga ttcgttgaag atgacgctct
     7621 gaccgctttt aatccacgga ttttctttcc cggcaatggg tttcaccaac agttgcaact
     7681 ggctgctgaa tacgccgcga tgatagtttt gataactcac ttccaggttg gattcaggag
     7741 ctgtcagttt tagttgcgcg ttcgcctgcg cgaccatgtc ttcgagatgg gtttcaatct
     7801 tcttgcctgt ataccatgcg ccgcctgtcc agactacgcc tagcgcaaca atgacgccta
     7861 ccgctaccag cgatttattc ataatgatta tccataaaat gaaatcaggc ggactggccg
     7921 cctgaaggtg ttataagcct ttaataagct tagcaagaga tgttaatttt ttcagtaagc
     7981 tcttacagct tgttgtaaac acgcgctaaa cggccgtggc ctttgacagt caccggtgat
     8041 tcgttggcgg caataaacgc tgattcaccc ggtttaagct gtaactgctg agaacctttc
     8101 cacaacgttg catcgccttc gacgcagaac aaaatggcgg cactctgctg gctaatggtg
     8161 gtttctttat cactaaggtc atgcagcgag aaggcaaaat catccactgg aatcgggaag
     8221 tccagttctg caccttgttt caccggctgg gtcaacaact ggttagccgg tttggcttcg
     8281 aatttcacat tggcaaccag ttccggaata tcaatgtact taggcgtcag acccgcacgc
     8341 agcacgttat cggagttcgc catcacttcc agcgccacgc cttgcaggta agcgtgcggt
     8401 gtttcagcga acaggaacat cgcttcgcca ggattcaatt tcaccacatt cagtaacagc
     8461 ggggagaaca ggccgctgtc ttccgggtaa aattcagaaa ttaaacgaat cgtttgccac
     8521 ggttcaccct gctgactgtc gagcgccgat tttaaaatcg ccagcgcgcg ggatttttct
     8581 tcaccctgca tattcaacag gctggcgaac agttcgctta aacgttcggc attaggctgt
     8641 tgtaaaaagt gagcaattgc cggatgtgca cctgcgactg gctggagtag ggagacaatc
     8701 tcggaaaact cacgaaacgc gttcatcgca aggaaaggcg tcagcgcaaa aaccagctcc
     8761 ggcttgtggt taggatcttt atagttacgc tcggcggcat ccatcgggat acctgcggca
     8821 ttttctttgg caaaaccgat ttcagaattg cgtttgtttg gatgaacctg aatggagagc
     8881 ggctgtgctg cgcataatac tttgaacagg aaaggcagtt cgccaaagcg tttggcaacg
     8941 gcctctccga gcagagtcga tttatcactc tcaatcacat cacgcagtga aacgatatct
     9001 ccggcggcat tctgcactcg tgaactgctt ttcggatgtg cgcccatcca cagctcggcc
     9061 atcggctggc tggacggatt ttccatacca taaagttcag tcaacgccgt tttgctgccc
     9121 caggcatagt tttgcactga gttaatgagt ttttgcatga tcaatccctg ttttaatgtg
     9181 gaaattaatc ccactattaa agcaagaatc ctacgggaag taacctggag ccgcaaaaag
     9241 tcgtactagt ctcagttttt gttaaaaaag tgtgtaggat attgttactc gcttttaaca
     9301 gggcaacgga acacccgccc agagcataac caaaccaggc agtaagtgag agaacaatgt
     9361 caaacaaacc ctttcattat caggctcctt ttccactcaa aaaagatgat actgagtatt
     9421 acctgctaac cagcgaacac gttagcgtat ctgaatttga agggcaggag attttgaaag
     9481 tcgcacccga agcgttaact ctgttggcgc gtcaggcgtt tcatgatgcg tcatttatgc
     9541 tgcgtccggc tcaccaacaa caggtggccg acattctgcg tgacccggaa gccagcgaaa
     9601 atgataaata tgtggcgctg caattcctgc gcaactccga catcgcggcg aaaggtgttc
     9661 tgccaacctg tcaggatacc ggcactgcga ttattgttgg taaaaaaggg cagcgtgtat
     9721 ggaccggtgg ctgtgatgaa gcggcgctgg cgcgcggtgt ctataacact tatatcgaag
     9781 ataatttgcg ctactcgcaa aacgcgccgc tggatatgta taaagaggtg aataccggca
     9841 ccaatctgcc agcgcagatc gatctttatg ccgttgatgg cgacgagtac aaattcctct
     9901 gtatcgccaa aggtggtggt tcggcaaaca agacgtatct ctatcaggaa accaaagcgt
     9961 tactaacgcc ggggaaactg aaaaattacc tggttgagaa gatgcgcacg ctgggtacgg
    10021 cggcctgtcc tccgtatcat attgcgttcg taattggtgg aacttctgca gaaactaacc
    10081 ttaaaacagt gaaactggct tccgctaaat actatgacga actgccaacg gaagggaatg
    10141 agcacggtca ggcattccgc gatgtggaac tggaaaaaga attgctgatc gaagcgcaaa
    10201 atctcggtct gggtgcgcag tttggtggta aatacttcgc tcacgacata cgcgtgattc
    10261 gcctgccacg tcacggcgca tcctgtccgg ttggtatggg tgtttcctgc tctgctgacc
    10321 gtaatatcaa agcgaagatc aaccgtcagg ggatctggat cgaaaaactg gaacataatc
    10381 caggtaaata tatccctgaa gagctgcgta aagcgggaga aggcgaagcg gtgcgcgttg
    10441 accttaaccg tccgatgaaa gagatcctcg cacagttgtc gcagtatccc gtttctacac
    10501 gcttatcgct taacggcacg attatcgtcg gtcgtgatat tgctcacgcc aaactgaaag
    10561 agcggatgga taacggtgaa gggctgccgc agtacattaa agatcatccg atttactacg
    10621 cgggtccggc caaaacgccg gaagg
//
LOCUS       DAJTYD010000135        10620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTYD010000135 DAJTYD010000000
VERSION     DAJTYD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.135"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            353..1711
                     /locus_tag="OO585_003050"
     CDS             353..1711
                     /locus_tag="OO585_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR6630556.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="OO585_003051"
     CDS             complement(1950..3335)
                     /locus_tag="OO585_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6630557.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSSREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OO585_003052"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OO585_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6630558.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="OO585_003053"
     CDS             complement(3729..4109)
                     /locus_tag="OO585_003053"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6630559.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="OO585_003054"
     CDS             complement(4464..4898)
                     /locus_tag="OO585_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR6630560.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="OO585_003055"
     CDS             complement(4886..5287)
                     /locus_tag="OO585_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630561.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="OO585_003056"
     CDS             complement(5547..6116)
                     /locus_tag="OO585_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR6630562.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6861..7004)
                     /locus_tag="OO585_003057"
                     /pseudo
     CDS             complement(6861..7004)
                     /locus_tag="OO585_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(7338..7604)
                     /locus_tag="OO585_003058"
     CDS             complement(7338..7604)
                     /locus_tag="OO585_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630563.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7745..7951
                     /locus_tag="OO585_003059"
     CDS             7745..7951
                     /locus_tag="OO585_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6630564.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8046..8648
                     /locus_tag="OO585_003060"
     CDS             8046..8648
                     /locus_tag="OO585_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630565.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            9848..>10620
                     /locus_tag="OO585_003061"
     CDS             9848..>10620
                     /locus_tag="OO585_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630566.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQ"
BASE COUNT         2547 a         2816 c         2672 g         2585 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttcg gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcttttt
     2461 ctgtccgggc tgtaagcgaa ccacactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc aatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 cgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagc
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ctgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttggg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtcg ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaatatgg ccggacatgg gatcgtcttt cagcgccgtt tgtactttcg cagccaggcc
     3661 gttgaagcta tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agcagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagctgcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggagg cagcaacgag cagctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgacactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata atcttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtttcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gccagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggt
     6001 acatccggga gacgcaggtc aacccgcaat gccgtcaggc ggggatgaac attcagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgtcgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gttctgtcga ccccttcggc tactaccgga
     6421 atacagaccg tatcgttacc atactgctgg cgctgattat tgtggtcctg ctgactactg
     6481 tctgcgtcag tgtgtgtata ctgtgtagct ggtgagataa cggttcatgt atcccctgag
     6541 ctgacagtgt accgtgtata ttaagcgtaa ccgacaggtt ctccttcata ccatattaaa
     6601 ttttccaggg attgccgctg ccttatttcg tcccatacag gaaaactcat ctgggtttct
     6661 gacaaatacg cgaaactcac agcccctcaa tatcaaccac acgttgtaag ccggtaatac
     6721 ctgaaaatca gcctattacc gcccacgcct ccggcaccat tcccttttga tttcgaattc
     6781 tttgttgtcg ctataccgct taatgttccc ggtcctccgg tgcagataac accggagtca
     6841 tcgtagcgct gcttcaatat tcaggtgacg tatttccaga cggaagccgg cgcgcgatcg
     6901 taaagtttgt tcattgtcag ttctgccagc cggttgtcaa cggcagagtt aaaggcttcc
     6961 agctccgcaa gggtaagttt ataacgcgtg tgggaattca cttttcccgc gtctcctgac
     7021 gtattacggg ctgccactta cctggtgtcc cgcaatacag ccttagtgtt ccgttatcgg
     7081 ggatccggac gcaccgcaac aaccggggaa gtatttctgg tttagagcag tgtcaagagg
     7141 gatttctcag cccctcactc accggctcag tctcagggca ggagcattaa tacgctcagt
     7201 tcatgtctta tacaggaatg ggcacagggg gagctggcgt gtcactgctg attttacgca
     7261 tcagcgttca tgtatctgca ctctgataca tgaacgattc acagcgctct gttaccggag
     7321 agatttactc tcttagctta ctctggcaaa tcctttacgt tactctctga tgacttctta
     7381 tatttataat accaatcaga cagccttatc ccatcggtga tatcagtctc ctcctcgcca
     7441 ggaacgggct gactcactgt aactacctcc gaaaacaact ctgcaattat cagaacgcct
     7501 gcttctctcc ctgtcctcac gaaaactatc ccctctttat cgcgcgtgcg tgcggaagca
     7561 tcttttcgca acaaccaccc ggtataccgg tacggctcag ccaccgcaac ctccccgttt
     7621 atctcctggc ggtcacattc ccgattattt tttacgtttc tccccggttg ttatgccggt
     7681 gaaggtggcg cgctgttttc atgaccacac cggctggttt aacaacatcc ggaggaacat
     7741 tctcatggct accccagttt cactgatgga tgaccagatg gtcgacatgg catttatcac
     7801 tcaactgacc ggcctgacag ataagtggtt ttacaggctc atcagggatg gggcctttcc
     7861 ggctcccatc aagctgggcc gcagctcccg ctggctgaaa agtgaagtgg aagcctggct
     7921 acaggcgcgt attgcacagc cccgcccgta atttctgacc gttatccgtt cacccgcagc
     7981 agacgtaatc cccggcctgc cggcggcatt ctgctgcctg ttatatcccc gtgaggaata
     8041 tgaaaatgaa acaacagtat cagacccgct acgaatggct ccacgaaagc taccagaaat
     8101 ggctgaccgg cttcacccgg cacgccgtat cctggggcgt gtgtcatccg aatatctact
     8161 acttccataa tctgacgccc ggttgggtgt cattcaatgg tgaaaaaccg gagattgcca
     8221 tcgtacccca gagcctgcac cggctgattt atggccctga caaacgggcc acgccgcccc
     8281 tggatgatga tctgatagtg aatttatgca ccagtgaaca tctgctggtt catcatccga
     8341 tgctggaagg cattctcctg tctgaatgtg aacgtctgag gcagcgttct ctggcaaata
     8401 aactgatcag cctcttccgt cagtttggtg gcacggagtt gcgcctcaaa ctggtctggc
     8461 tttgctggct tgatttaatg accggaaaca gccttgaaga ctggaaagag aacctgaaac
     8521 ggaaatcaga aaaagagctg gaggaatgga tcatcaaccg tcagaggcag agcgcagcac
     8581 tgacggatct gatggatcag tacgtgctcc tggcataccg cacaacggtt gacgatagcc
     8641 gcaactgatg ccggttgctt ccggaaggtg tgacaaaata cggaaatgat gatgctcccg
     8701 gcccccatgc tcacctgcag agcgtgttcg tcgtcatcat actgagccat gacgggcgtc
     8761 ttaagggcat cctgtcattg ctgagcaggc aacaccacca gaggagccac ggacagggat
     8821 gttctttaca gcccgataca cgccaggttc tcagcagact gaatgtgcag gtgcggttat
     8881 tcaccagaaa tggcatgctg cggtacatac ccatcctggt gacaatatgt acgtggcgac
     8941 agcgatcccg gcactgtgga agagactcac tcagcctgca ggatattctt tccggcatcc
     9001 gggaatgtcc gcctgaatgc aaacaggatg atatttgaat ccgtaatact gttctttcat
     9061 cataaataat ttatgcagat acagttaacc gttacagtag ccggtttgtt ttaccgggaa
     9121 ataatatccg tcagactgat accggaacat atctgaaaca cgttatgatg ttttgttatc
     9181 agaaatgttg tatattctta tccgtggcga cagtttaatg ccctctggta tcacctgata
     9241 atgtatatcc tgcgatgatt cgttatattt acgggacacg cctcttttca ttgtgctcct
     9301 tttcagactt cattttccgt aagtgttccc cggcataaag gaggtacctt tctggcgttt
     9361 ataactctct ttatcggtga cgccggtaat acagggaata ccacaccgac aaacgaggta
     9421 ttaacagacg atatttcagt gtccataatt tttctgtccg gttaaataac gtcagtctgc
     9481 tgccctctga ctgtaatcat caaagccgga atgcataccg ggttacaacg taaaaaaagt
     9541 gatgctggcc cccggtgcag ctggcgggcg aaacatgttt acctttcaat accccctgtc
     9601 tgcagtctcc actcttccat cctcttaagc tacgtcggcc acatgagtgc acttccatga
     9661 taacaggtat tcctgtcagc ataacgaagc cggttacctt cccttcctcc tgcatttcct
     9721 gtcagaatga ctatacagcg cgcagattgc ctttttattg accatcttaa tataccgtta
     9781 taaacttctt tttatcatat tattatcacc ttattatgtt taatttttaa gcagtcggag
     9841 aaagaggatg gttcagaaaa ttttgtcgga taaggttatg aatgaaagaa caaatgcgta
     9901 ttactcttat tatcttggag agcgtaatat ctccgtactg ccccttaatg tttatgatcc
     9961 cccggaacgt tttatcgctt acataaaaaa aaacagagaa aatctgaaca tcacactttc
    10021 agattttgaa cttgaacaaa taatttctgg tatgcgtctg aaggccctgg ccttcctggt
    10081 ccccctggaa aaaatatcct ggatcgcggg cagtgaacgc gcatgcttat tttcctggta
    10141 tctgttgatg cagtttatcc agaataacag ggcaaaaata agcgcagact tgcttcaaaa
    10201 gaataaactg tatcttaaag aagagtatct ggaagggaat gccttcccgt cggactcatc
    10261 gacacagttc agacaaatac tcagagttct cgatattctt tctgataaga atttgcggga
    10321 tgagtggata atccagacca aagacagatg gatgcgggca tttaaatcaa agagtccttt
    10381 cagttacctc ctcccggaga acgaacatga atgcatatgg acatggaatt atttgaaggg
    10441 aaaaaatatt gcactggaaa aactggccag ttttcctggt tctgctgata tttaccacgc
    10501 aattcacctt tcatttgaca tatgggttac ctgtccactc acctcacctg acgatataaa
    10561 aaatttcaga aacagtttca ataaggccaa ggcgcagcgc aagtacaaaa aaatgcagga
//
LOCUS       DAJTYD010000136        10606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTYD010000136 DAJTYD010000000
VERSION     DAJTYD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.136"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1806)
                     /gene="gss"
                     /locus_tag="OO585_003062"
     CDS             complement(<1..1806)
                     /gene="gss"
                     /locus_tag="OO585_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6630567.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDES"
     gene            2011..2877
                     /gene="yghU"
                     /locus_tag="OO585_003063"
     CDS             2011..2877
                     /gene="yghU"
                     /locus_tag="OO585_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6630568.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3000..3248)
                     /gene="hybG"
                     /locus_tag="OO585_003064"
     CDS             complement(3000..3248)
                     /gene="hybG"
                     /locus_tag="OO585_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6630569.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3261..3602)
                     /gene="hypA"
                     /locus_tag="OO585_003065"
     CDS             complement(3261..3602)
                     /gene="hypA"
                     /locus_tag="OO585_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6630570.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3595..4083)
                     /gene="hybE"
                     /locus_tag="OO585_003066"
     CDS             complement(3595..4083)
                     /gene="hybE"
                     /locus_tag="OO585_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6630571.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4076..4570)
                     /locus_tag="OO585_003067"
     CDS             complement(4076..4570)
                     /locus_tag="OO585_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6630572.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4570..6273)
                     /gene="hybC"
                     /locus_tag="OO585_003068"
     CDS             complement(4570..6273)
                     /gene="hybC"
                     /locus_tag="OO585_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6630573.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6270..7448)
                     /gene="hybB"
                     /locus_tag="OO585_003069"
     CDS             complement(6270..7448)
                     /gene="hybB"
                     /locus_tag="OO585_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6630574.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7438..8424)
                     /gene="hybA"
                     /locus_tag="OO585_003070"
     CDS             complement(7438..8424)
                     /gene="hybA"
                     /locus_tag="OO585_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6630575.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8427..9545)
                     /gene="hybO"
                     /locus_tag="OO585_003071"
     CDS             complement(8427..9545)
                     /gene="hybO"
                     /locus_tag="OO585_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6630576.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9734..10021)
                     /locus_tag="OO585_003072"
     CDS             complement(9734..10021)
                     /locus_tag="OO585_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6630577.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10140..10606)
                     /locus_tag="OO585_003073"
                     /pseudo
     CDS             complement(10140..10606)
                     /locus_tag="OO585_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2553 a         2942 c         2656 g         2455 t
ORIGIN      
        1 tgattcatca ccgcgcaaac acgtcccacc gtagttgccg cccacggtga aggtacatac
       61 ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt tgctgataga tgtttttctg
      121 ctcggcaaat ttaccgctgg ttttgtccag cacctcttca tgatggctga ccaggtcgat
      181 attgctacca cagcgaccgg caatcggttt cactgcgtaa cctgttttca ccaattcatc
      241 attaacagtg aaatcggtat ccagcaggta acgatggtgc gggaacagcg accagaggat
      301 cggcagaatc gctttgttgc cggggatcac cgtccacagc ggctcaaaga ctagcacttc
      361 cgggcgcagc aatacgtcga taagacgcac ttcgttttgc ggatgaccgg tacggattgg
      421 caccgcagca aactcacggt cgctaacttc acgaatctga tcaaacgcgg tttcccacgc
      481 ccaggttttc cacacgcagt taaccagtcg cccttcccca tcaatcagtt gcccggcagc
      541 atcccagccc agttcatcca atccacgcaa gatacgcgtt tcaaagcccg cctggtgcag
      601 cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata tctttgtcct gcatgatatg
      661 gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc aattcgttaa tcagcccttc
      721 cgccggattg aagccgttgc ctttatagcc ctgctccgcc caacgttcga ggatcaagcc
      781 cgcttcggta tgacaggagg cggagtcggc gttgtactcg taaaccttca ggccacgctc
      841 atccatgcag aaatccatac gaccagtgat catatggtga cggcgacgct gccaggagag
      901 acgcaaacgt ggccagagga ttttcggaat gtcgaacagc gccagcaggt tgtcatcttt
      961 cagtaccttg tcggttgcgt gaagatacat caggtgcagc tcgttggtgg ctttaattag
     1021 ctcctgctcg gcactctcgg taatggtgta gtaatgataa ggatcctgat tgatcacctg
     1081 accgttggcc tgcacatagg cgttttgcag cggatctttt tcatccagcc atttaccgtc
     1141 aaactggcct ttgttttcca ggcgcgctcc gctgattttc agcagctcgc ctgcaatttc
     1201 cggctgcggt aagctgtatt cagtatcttc cgtctggatc atccagccca gaatggtggt
     1261 gtcatcaaaa gtgtctttca gggtatagca gccgttttcg accaccatct ccagctcacg
     1321 cgtccactgt tgcccttgcg gcaacgggga atgaatcacg ttctgttccg caatacggac
     1381 tttgttgcca tgcaattggg taatgatggc gacatggcca gtgtctttaa attcaccgcc
     1441 tttatcccag ataagaagcg cacccgcgac cggcgcacgc ggcgagccgt taggaaatgc
     1501 ctgcaatggc aggatgttgt cattaaccac ttctcgcagg aagcgcagcg agaaaatctc
     1561 ccacgccata cccacgtcag taaagaccac accgtaattc agaaagagaa aacggcgagc
     1621 aaattcaacg cattgccact tgtggcccat atattcgtcg tcgatatagc tacggaatac
     1681 ggcgtcatct tcgtattcct gcggatcgag agaactgtaa tctgaagagt agattgctac
     1741 cccacctggg gcgtagccca ataatgtccc gaacggggca tcctggctgg tcgttccttt
     1801 gctcatcact ttacctttaa caatacaacc taagcagttg gcgtaattgt tgtgctctga
     1861 ttacctgctc agcgacatta accggacaga ggtcagacta atcatacacc tcatcgcagc
     1921 ggctggcgca aagggttaaa aaattcacat tctgtacagc aagtgacctg ccacactgct
     1981 tcaacactac cactcagaag gcaactcact atgacagaca atacttatca gcccgcgaaa
     2041 gtctggacgt gggataaatc cgctggcggc gcgttcgcca atatcaatcg cccggtttct
     2101 ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc cattgcaact ttattcgctg
     2161 ggaacgccga acggtcagaa agtaacgatt atgcttgagg agctgctggc gctgggcgtt
     2221 actggtgcag agtacgacgc ctggctgatt cgtattggcg atggcgatca attctccagc
     2281 ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc tgcgcgatca tacgcataat
     2341 ccgccgatct gtgtgtttga atctggttcg atcctgcttt atctggcgga gaaatttggc
     2401 tacttcctgc cgcaggattt ggcaaagcgt actgaaacga tgaactggct gttctggtta
     2461 cagggcgcgg caccgttcct cggcggtggt tttggtcact tttaccatta cgcaccggta
     2521 aagattgagt acgccatcaa ccgctttacc atggaagcca aacgtctgct cgacgtgctg
     2581 gataagcaac tggcgcagca taagtttgtt gcgggcgatg agtacaccat tgcggatatg
     2641 gcgatttggc cgtggtttgg caacgtggtg ttaggtggtg tgtatgatgc cgctgagttt
     2701 cttgatgcgg gcagttataa gcatgtacaa cgctgggcga aagaagtagg cgaacgtccg
     2761 gcggtgaaac gtgggcgtat tgttaaccgc accaacggac cgctgaatga gcagttgcat
     2821 gagcgccatg acgccagtga tttcgagacg aatacggaag ataagcgtca ggggtaaggg
     2881 ttggtgttcg tcgcagcaag ccatccaggc cggataaggc gttcacgccg catccggcaa
     2941 tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca ggcctacgaa agctaatcat
     3001 cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt ctaatgtggc tttggcttca
     3061 tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca gcacccactg gcccagtaga
     3121 tcggcagggt taccttcaca aatcagggcg atattcacgt cgcgcttgat accacatact
     3181 tcaacctgcg caagctggtg aatatcttca ccgacagcca gcacctggcc tggaacgcca
     3241 atacacatta ttaactccgg ttattcaact tcaatacttt tgacgatcag cgaatcgccg
     3301 gtatcgacac gcaaccgctc gccgtgacag agcggacact gcgcatcgtg ctgatgaatc
     3361 tccaccacct ggctgcaatc ccagcaccag gcctgggcgg gtttatagac gatatgtaaa
     3421 tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa aactaaaacg gacggcgctc
     3481 tcctcaacgc aggagagcgc gccaatttcc agccacacgg cggtgacgcg cttaacatcg
     3541 tgctgctccg cctgccgttg gataatttca acggcgctct ggcaaagcga caactcatgc
     3601 attttcgcca ctcctgcgac caaacagcag ggcgcgacgc cctgcgtgtg gtacatccgg
     3661 attcgtgact ggcagcgaaa ggatcattcg tgcgcagtca tccgtcaggc gttggccctc
     3721 ttcaatcgac atactgtgcg aaagcggtga catcagcgaa caggagaggt attgcgaaac
     3781 accgtccagt tcgccaacgg taaaggtcat ggtgccatac ggcagttgca gaccaatttt
     3841 ttcactcact ttgcgcagcg gccagagttg atccgggccg gggaagataa ctgcactcag
     3901 catccacggg gtgatcacac accccgtcca ctgaccttcg aacagcgtaa aatcagaaac
     3961 atacaccggc attgaaggat gcagaaaaga gagatcgtgc atcgaacgcc gggcaatttc
     4021 ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg aaacctgcta tctcttcagt
     4081 catgaatcgc ctcccgtggg atggcttcca cgcctgattc gcgcagcgca gccagaacct
     4141 gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt taagccgatg tgtggctcca
     4201 gcgattccgg gatcacgccg accagggtca gctttttcgg aaactcaccg gtgaagcgca
     4261 gggccgacaa tacgtcggcc aggccaagct gatgcggaga gattttgttg gtaaacaacg
     4321 ccggaacttc ttcatcccgc aggatcatca tcgttcccgg cgtgttcttt ttcgacacaa
     4381 tggcatccgc aataatcaga tgatcgcgat ttgccatgtc gccaagcagc tccattcccg
     4441 ccgtgccgcc atcgaggatc tcaacataat ccggcagaat gtatcgttgc tctaacgctt
     4501 cgacaatccg cacaccgatg gcttcatcgg tcagcaaaat attgccgacc cctaagacta
     4561 aaatacgcat tacagaacct tcactgaaac cacttcgttg ccgtcggcat ccactacgtg
     4621 taccgcacag gccatgcacg ggtcgaagga gtgaatggta cgcaccactt ccagcggttt
     4681 attcggatca gcaaccggtg tacccaccag cgactgctcg taaggaccga cgtcatcatt
     4741 gaagttacgc ggaccagagt tccaggttga tggaacaact gcctggtagt tactgataat
     4801 accgtcttta ataaccatcc agtgagagag cataccgcgc ggcgcttcga ggaagccaac
     4861 acctttgaac tcacccgttg ccggaatgtt cggtttcaca aaggtggtgt gatcgccttt
     4921 gccgatattg gtgatcagtg cactgtattg attttgcagg atatcctgca attcgcagca
     4981 gtgaacggta cgaccaataa tacggcccag cgtggagtgc agctgcgcca cttccagcgt
     5041 gttgccagtc agtttctgat aaatcgcaac gatttcattc agtttgttct gggtagattc
     5101 gcgacctgcc gccagtttca ccagcatatt agccagcgga cccacttcta ccgttttgcc
     5161 gtagaaagtc ggtgatttca cccaggaata tttcccgtcg tcagaccaac catcataagc
     5221 cggaatggtg gtaccttccc acggtgcctg cggcgcttcg tctttatacc aggagtgctt
     5281 cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa tgagaagtga tcggacgata
     5341 cgaggacaga tccgcattct caatgtagcc gcccgggaac aggaagctgc cgtttttact
     5401 gtcggtcggg aattccggca cgctcaggta gttcaccgca cctttaccgc gcgtcagcca
     5461 ttccgggtag aacgcggcaa taactgcggt atcaacctta taaacctgtt caacaaagtc
     5521 gctcagcttg tcgatgaaag acttgatgta catcaggcgc tcaaggttca gcacgcccaa
     5581 accgtcgagg ttgatcgggt tcgcgacacc acctaccgcc aggttctgaa tgtgcggcgt
     5641 tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc tggcactcca gcgcctgcag
     5701 gtagtgcgct accgcaatca ggttcacttc cggcggcagt ttcatcgccg ggtgacccca
     5761 gtagccattg gcgaaaatac ccaactgacc gctggcaacc agatctttga tcttgttctg
     5821 aactttagtg aactcttccg gactgttcag atgccaggtc gaaacgcctt tcagcatttc
     5881 ggaggctttg gttgggtcag cttgcagtgc agaagtgatg tccacccagt ccagcgccga
     5941 aagctgatag aaatgaacga tatggtcatg cgtggtgtgc gcagccagaa tgatgttacg
     6001 gatgtattgc gcgttaaccg gaacgtcgat attcagcgca ctttctgccg cacgaacgga
     6061 agacagcgcg tgagtggtag tacatacgcc acagatacgt tgcacaatca tccatgcatc
     6121 gcgcggatcg cggtttttca cgatctcttc catgccgcgc cacatggtac cggaagccca
     6181 tgctttcgaa acgacgccat tttcgatttc gcaatcgatg cgtaaatgcc cctcaatacg
     6241 ggttaccgga tcaatagtaa ttctctggct catgctttgc tcgcctcatg acgattatga
     6301 tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa tgagtacgat gtaagcgcaa
     6361 atctcaatag ccacaaaacc aatagaaatc aacagttctt ccctggtcgg gaagtaggcg
     6421 taaccgccgc ccgggttgaa tgccaccagc gaataggtca gacgccaggt tgcacaacct
     6481 aacagtgcgc tcagtgctga caggaacagc atgcgggaat cattacgcag cttcgccaca
     6541 cgcagaacga ccagcgggaa gagcatcagc aggacttcaa tccagaacat cacggagtag
     6601 aagtcaccgg caaacgctaa cgacagcttg tcgcgataga taagctcgcc aaagcgcagc
     6661 acgataaaaa tcgccagcaa cacactgatg gtgttggtca gcttgacgaa cagactcttt
     6721 tcatccggac cgttgccacg cagacccgcc tgcaccagcg aaccttcaaa gatgacaatc
     6781 gagaagccca tgatgaacgc cgtcagcagc gagaacagcg gcaacatttc atagctctgc
     6841 cacaacggat gcaccttgta gcccgccgag atcatcagtg accccattga agactggtgc
     6901 atggtcggca acagcgcgcc aagtgcaatg atgaagaaca tcaccttgtt cagacgcttg
     6961 agagaaacct tccagcccag acgttcaaac agtgccggag caaactccag tgccatcacg
     7021 ccgatataga tggtcataca gaccgccgtc tcgaacagta ccgagttcac gttgaagtga
     7081 cccggaatgt agaagtacgg caggttccag tagcgaccca cgtcgatagt gatcgacaag
     7141 ccacccagtg agtaaccaaa cagactcgcc aacagcgccg gacgcaccag cggatggtat
     7201 tgcccacggt taaagacgta taccgcccac gccagcgccc agccgccaca ggcaaagccg
     7261 gtaccaatca gcaggtcaaa cgcgatccac acgccccacg ggaagccgcc gttcaggtca
     7321 gagacagagc ccagaccgaa caccagacgc ttcacaatca ggagcataca gatgacgatt
     7381 aacggtccaa aaatcatgac cggtttactg atgattttgc cgcccagcgg ttgtggatca
     7441 tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg gtgttgcgac gaaccagcac
     7501 ggttaagccc gccagcacag ccagtggtag catcatgcct ttatacaggg tgtgttgaat
     7561 attttcggaa cgcgcaccgg tagaaagatc gtccagtttc ggcaggtcga gattttcata
     7621 aggcacaccc gtcagtacca gaacctgagt accgccgccc tctttctcgc cgtacagatg
     7681 cggataatat ttcggcaccg tatgcaggta agtgtcgcca gatttcagcg tctgacgcgg
     7741 atagtggtat tcgctgccag gcttcagcgc cagacgtttt ttcgcctccg ccatcagctc
     7801 ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact tctacgcagc caggcagacc
     7861 gcctttatcg agacgttcca cacctttctg gttgcacagc tcgcacttat gcagcgcacc
     7921 aaacgggttg ttgtagtcgt acttcggcac gttgtacgga caggcgacca tgcagtaacg
     7981 gcaaccggtg cacacgtctt tgtcgtaatg gacaatgccg gttttcggat cttttttcag
     8041 tgcagagacc gggcacacag agacacagtt cggatcgacg cagtgcatac actgtttctt
     8101 aatgtacgcg tagccgttct cctcctggtc tttgttgacc cctgtgccgc tggtccacac
     8161 ctgaatgatg ttattggtat acggcgacag tttgtcgttg ttcgaccagg tctgttcccc
     8221 ttgcgggtta cgttcaggga agttgatatc ctgacacttg gtgacgcaag cctggcagcc
     8281 tacgcacaag gtcgagtcgt acaacatccc cagcgatccc ggaattggcg ggcggttttc
     8341 agcagccgca tgactgacag acggcagcgc gcccgtcagc aatgccccgc aggaggctgc
     8401 tttaataaaa ttacgtctgt tcacggttat tctccccgtg agtcagcgtt atctttcttt
     8461 tgctgacgac ccagttcacg caccgccatc acgctgacac cggcaaccag cccaaccaca
     8521 ccgccgagca aaccaatagc gcctgcagag acgttaccgc cctctttagc gttaacatcc
     8581 ggtttctgtg aacgcggagt ctgattttcg acgttggcaa gctgatggat gcctttatgg
     8641 aagccgatac cttcttcgtt acagccatag catgggtgac caatcgctac cggccacacg
     8701 ccgccaacat cgcagaattg cagcgttgag cagttgccgt aagtttctgg ccctttacag
     8761 ccgaggtggt acaggcacca gccttcacgg tggccttcat caccgaactc tttggcaaaa
     8821 cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt gaatcagacg gccataggcg
     8881 aaggtcggac gatttttgtc atccagtttc ggcggtttgc cgtaagtgat gatgtgcgca
     8941 acggtcgcga ggaagttgtg cgggttcggc gggcagcccg gaatattgat aacggttttg
     9001 cctggcagaa cttcttgcag gctgactgcg ccagttgggt taactccagc tgcggcaaca
     9061 ccgccccacg cagagcagga accgatagcg ataatcgctg ctgcgccttc tgccgctttg
     9121 cggatgtgat ccacaatcgg ctcaccagca accatgcaat aaataccgtt atcttttaat
     9181 gggatggaac catccacaac caacacatac tgccctttgt acttctcaag tgcgttatgt
     9241 ttgttctctt cgacctgatg accgaaggcg gcggaaagca cttcgtgata ctccagagag
     9301 atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg cacgaagcag agattccgta
     9361 caaccggtgc actcctgcgc gccaatccag ataactggcg gacgctgcgg gttagtaacc
     9421 gattcggcca tctctgcagc ggctttgcta cttaacccca tggtggcggc taatgctgca
     9481 caaagcttca tgaaatcacg acggttaatg ccgtgagaat ggatgagggt gttatctcca
     9541 gtcatttata gttattccgt tgcgaagacc tggcatatat tttgcctcaa tcgcaaaatc
     9601 aataatgcga tcgatgcgcc atttaccaca catttattat ggttatcgat atcatgatac
     9661 tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt aattaattaa agcaagataa
     9721 tacgtatgtt tgatcaattt tcgaggcgat aaccggccat aaaaaagcgc aacgtagaac
     9781 aggaattatt ctcacgaaag aaatccatta ccatctcttt atccagtcca tagcggcgcg
     9841 tcagaatacc cgcttcccag gcgctaagct ggcgatctcc ggtcttttcg tacatccggt
     9901 gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca gaattttgtc acattaaccg
     9961 cactgtcggc atgcgttgct tttaatcccg ccattaagcc tttaccaaaa tggttattca
    10021 tcgttcacct cattcgctat atattatcat atatagcaat attttaggca aagaacgact
    10081 tttgcgcacg ctattttttc atgcttgttt aagacgtaat cccgtaagag ataaacccat
    10141 caggagagat atttatcgaa ccatttcagc gtcctttgcc aggcaagatc ggcggcagat
    10201 ttgtcataac gaggcgtgga atcattatgg aatccgtgat taacccccgg atagatatac
    10261 gcctcataga ccttattatt ggctttcaac gccgcctcat aagcgggcca gccctcgttg
    10321 attcgggtgt ccagttccgc gaagtggagt agtaaaggcg cttcaatctt cgccacatcg
    10381 gcagtgggtg cctgacgacc ataaaacggc accgcgcagg ccagttccgg atacgcgaca
    10441 gccgccgcgt tcgatacacc accgccatag caaaatccgg taatacccac ttatgcctgt
    10501 cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca tcagtttggt
    10561 tggatcaacc tgctgttgca gctcacgacc tttatcatca tttccc
//
LOCUS       DAJTYD010000137        10241 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTYD010000137 DAJTYD010000000
VERSION     DAJTYD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.137"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            158..505
                     /locus_tag="OO585_003074"
     CDS             158..505
                     /locus_tag="OO585_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6630578.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            495..857
                     /locus_tag="OO585_003075"
     CDS             495..857
                     /locus_tag="OO585_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6630579.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            854..1351
                     /locus_tag="OO585_003076"
     CDS             854..1351
                     /locus_tag="OO585_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6630580.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1359..2543)
                     /gene="emrD"
                     /locus_tag="OO585_003077"
     CDS             complement(1359..2543)
                     /gene="emrD"
                     /locus_tag="OO585_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6630581.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(2962..3051)
                     /gene="tisB"
                     /locus_tag="OO585_003078"
     CDS             complement(2962..3051)
                     /gene="tisB"
                     /locus_tag="OO585_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6630582.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            3616..3714
                     /gene="ivbL"
                     /locus_tag="OO585_003079"
     CDS             3616..3714
                     /gene="ivbL"
                     /locus_tag="OO585_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6630583.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            3820..5508
                     /gene="ilvB"
                     /locus_tag="OO585_003080"
     CDS             3820..5508
                     /gene="ilvB"
                     /locus_tag="OO585_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6630584.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQDIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5512..5802
                     /gene="ilvN"
                     /locus_tag="OO585_003081"
     CDS             5512..5802
                     /gene="ilvN"
                     /locus_tag="OO585_003081"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6630585.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            5876..6466
                     /gene="uhpA"
                     /locus_tag="OO585_003082"
     CDS             5876..6466
                     /gene="uhpA"
                     /locus_tag="OO585_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6630586.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6466..7968
                     /gene="uhpB"
                     /locus_tag="OO585_003083"
     CDS             6466..7968
                     /gene="uhpB"
                     /locus_tag="OO585_003083"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6630587.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            7978..9297
                     /gene="uhpC"
                     /locus_tag="OO585_003084"
     CDS             7978..9297
                     /gene="uhpC"
                     /locus_tag="OO585_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6630588.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9435..>10241
                     /gene="uhpT"
                     /locus_tag="OO585_003085"
     CDS             9435..>10241
                     /gene="uhpT"
                     /locus_tag="OO585_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6630589.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANI"
BASE COUNT         2267 a         2683 c         2874 g         2417 t
ORIGIN      
        1 cctcgtcctt cagggcgtgg aggatgtcaa tttgcgctat gatacccatc tgacctcctt
       61 gtagcggatg gcaaaagcat atcaggaaac gggcggacga aattcttaaa aaaaaccgct
      121 aaagatagag cgttagtgaa attgcaaagg agcaacaatg aaaatttccc gccttggaga
      181 agcgcccgat taccgcttct cgctggctaa tgagcgtact tttctggcgt ggatccgtac
      241 cgcactagga tttctggcgg cgggggttgg gcttgatcaa cttgcgccag atttcgccac
      301 gcccgtcatt cgtgaactgc tggcgttgtt gttgtgtctg ttttccggcg ggttagcaat
      361 gtatggctac ctgcgctggt tacgcaatga aaaagcgatg cgtctgaaag aggacctgcc
      421 ctacaccaac agcttattaa tcattagctt aatattgatg gtcgtggcgg tgatcgttat
      481 gggactggtg ttgtatgccg gatagccgca aagccagacg cattgccgac ccaggactgc
      541 agccggagcg cacatcactg gcgtggtttc gtaccatgct gggctacggc gcgttgatgg
      601 cgttggctat caaacacaac tggcaccagg cgggcatgtt attctggatt tcgattggca
      661 ttctcgccat cgtggcgctg atcctctggc actacacccg taatcgcaat ttaatggatg
      721 tcacgaatag cgatttttcc caatttcacg tagtccgtga caaatttttg atctccctcg
      781 cggtgttatc tctcgcaata ctgtttgctg taacgcatat acatcaactt atcgtattta
      841 ttgagagagt cgcatgacag gaagtcaggt cattgatgct gaagaggaca ggcataaact
      901 ggttgtagag tacaaggatg ctctgcaacc tgccgatttt tatcacaatt tcaaacagcg
      961 gggcatccgc tccgtacagc tcattcccta tcttgaattt gatgaccggg gcgatctgac
     1021 ggccgcttcg gtgacagcag agctgtgggg gaaattttta attgccctgt tcgagtgttg
     1081 ggtgcgggca gatatcagtc gtatctcgat tgagcttttt gacgccacct tacaaaaatg
     1141 gtgtggcggc gaaaacccgc aaccgcgccg tgactgccag gcgtgcgact ggcatcgcct
     1201 gtgcccgcac gctcggcagg agacgccaga cagcgtgctt tgtgcgggct atcaggcgtt
     1261 ttattcttac tccgcaccgc atatgcgggt gatgcgtgat ctgataaagc agcatcgttc
     1321 tcccatggag ctgatgacga tgctgcggtg acgtgcgctt aaacgggctg cccctgatgc
     1381 gacatccgcg tcgccagcgg cagccagcac agcacgatca acaatcccat taaggtcatc
     1441 aataacccca ggctaccctg accggtttgc ggcaacatcg cagagagcga cgccagcacg
     1501 ccggaaccaa tgttttgcag accgccgacc agcgcgccag ccgtgcccgc caggaagggg
     1561 aacggctcca tcgcgccgct ggtcgccagc ggaaacagca tcccggcacc gaaaaagaac
     1621 agcgcggcgg gaacgagcag cgtccagaca ttcatcacgc caaaccagtc ggggatccac
     1681 atcagcaagc cagccagcag gcagcagata accgactgcc acattaacgt ggagaagcgt
     1741 ttattgggac gtccggcaaa ccatgcgcca aaaaacgctg ccggaatcgg cagaataaac
     1801 aaaatactga ccgtcatact gctcagccct aacaccgcgc ccatcagcac gccggagcag
     1861 gcttcaaagg cggcaatccc ggccagaccg ccaatcagca tcagcaaata acagttaaaa
     1921 ccgctgttac cgaaaagcgt tttataactg gtaagcaggc gcgtgcgcgg tgcatcgacc
     1981 ggacgcgttt ccggcatcca gcgggccata ctgaaggtca caccaacaca aagaaccaac
     2041 aaaaagagat aacaggcgcg ccagttccac atcgtatcca gcagaccgcc gattagcggt
     2101 gcgagcaacg gactgacgag aatccccatg tttaacaggc tgttagcatg gcgcaactgt
     2161 gtccgttcat ataaatctcg cggcaaagta cgcgccatta cgccgccaac gccggtgccc
     2221 atcccctgca tcgcgctggc ggcaatcaat accgtcaaac tggaggtcgt gaccgcgacc
     2281 agcgttgcca gcataaaaat ggacattccg acgaggatca ccggtcggcg acccacacgg
     2341 tcggaaatcg ggccataaaa cagctgtgag acaccgtaag tcagcagata agcgcccatt
     2401 acgctctgca ccgccccttc acggacgttg agatcgcgcg ccatatcggc aatagctgga
     2461 atataaatgg tttgcgccat ctgaccgacg gccacgagta ataccaacat caataacaaa
     2521 ttgacgtttc tttgcctttt cattatcacg gatgctttta taaaaataat tagaataagt
     2581 cactacgcat tcccataaat gcgggagagg aatctatcac attgatgaat gttagccaga
     2641 tatacgccag gaatggggaa ttgtttagcg gcagaatatg taaacaaaag cggcaataaa
     2701 tgttgtcggg agggattacg tgcgaactgt aaccctctcc ccaaaggggc gagggggaaa
     2761 ccaccgttcc ctttcagaaa atgagctaaa cattgcacgg gacggtcccc tcgccccttt
     2821 ggggagaggg ctagggtgag gggcacaggc agcgcaggtg caaacattct gctggtagaa
     2881 gaagccccca actccaaccc gcacgctaaa tataaaaggg gagcggttac ccgctcccct
     2941 ttggtgcgac ttgaatctga attacttcag gtatttcaga acagcatcaa gcagttgcag
     3001 tgctgcaaca atgagtttga ggataagaat ggcgatatcc accaggctca tacgcgtctc
     3061 ctgtggttca ggagcactgc cagctgacgt acctttccgc tgcctgttgc cagctgttgt
     3121 gcacgcggta tctcaaggag aaacgttgtg cttgttgaca taacacagtg tgctcacgcg
     3181 gggttaaggc gctgacggca ccacccgttt cagccaggac ttagtgcgcc gggtaacgat
     3241 gcggcccccg cataatacat tgcgtacagt gatattatag aagtttactg tataaataaa
     3301 cagtgatatt tggacaaaac gcaaactgtg atcgatactc aactatgccc taaatacgtc
     3361 aaaattcgtg ccgaaattgc gcgttctgcg cggaacacgt atactttcag tgttgacata
     3421 atacagtgtg ctttgcggtt accagctgca ggcggctgat gaaacctcgc tccggcgggg
     3481 ttttttgtta cctgcaattc agtacaaaac gtgatcagcc cctcaatttt cccttgctga
     3541 aaaattttcc attgtctccc ctgtaaagct gtgcttgtat aaatattgtt aaacacaaaa
     3601 ccaacaaggt ccccaatgac tacttccatg ttcaacgcaa aactactacc aactgcgcta
     3661 tccgccgcag tggtcgtcgt gcgtgtggtg gtggtcgtcg gcaatgcgcc gtagggactg
     3721 gaacaacaca cgattccaaa accccgccgg cgcaaaccgg gcggggtttt tcgtttaagc
     3781 acctcccgga aagtcggccc agaagaaaag gactggagca tggcaagttc gggcacaaca
     3841 tcgacgcgta agcgctttac cggcgcagaa tttatcgttc atttcctgga acagcagggc
     3901 attaagattg tgacgggcat tccgggcggt tctatcctgc ctgtttacga tgccttaagc
     3961 caaagcacgc aaatccgcca tattctggcc cgtcatgaac agggcgcggg atttatcgct
     4021 caggggatgg cgcgcaccga cggtaaaccg gcggtctgta tggcctgtag cggaccgggt
     4081 gcgactaacc tggtgaccgc cattgccgat gcgcggctgg actccatccc gctgatttgc
     4141 atcaccggtc aggttcccgc ctcgatgatc ggcaccgacg ccttccagga agtggacacc
     4201 tacggcatct ctatccccat caccaaacac aactatctgg tcagacatat cgaagaactc
     4261 ccgcaggtca tgagcgatgc cttccgcatt gcgcaatcag gccgcccagg cccggtgtgg
     4321 atagacattc ctaaggatgt gcaaacggca gtttttgaga ttgaaacaca gcccgctatg
     4381 gcagaaaaag ccgccgcccc cgcctttagt gaagaaagca ttcgtgacgc agcggcgatg
     4441 attaacgctg ccaaacgccc ggtgctttat ctgggcggcg gtgtgatcaa tgcgcccgca
     4501 cgggtgcgtg aactggcgga gaaagcgcaa ctgcctacca ccatgacttt aatggcgctg
     4561 ggcatgttgc caaaagcgca tccgttgtcg ctgggtatgc tggggatgca cggcgtgcgc
     4621 agcaccaact atattttgca ggaggcggat ttgttgatag tgctcggtgc gcgttttgat
     4681 gaccgggcaa ttggcaaaac cgagcagttc tgtccgaatg ccaaaattat tcatgtcgat
     4741 atcgatcgcg cagagctggg taaaatcaag cagccgcatg tggcgattca ggcggatgtt
     4801 gatgacgtgc tggcgcaatt gatcccgcag gtggaagcgc aaccgcgtgc agagtggcac
     4861 cagttggtag cggatttgca gcgcgaattc ccgtgtccaa tcccgaaagc gtgcgatcca
     4921 ttaagccatt acggcctgat caacgccgtt gccgcctgtg tcgatgacaa tgcgattatc
     4981 accaccgatg tgggtcagca tcagatgtgg accgcgcaag cttatccgct caatcgccca
     5041 cgccagtggc tgacctccgg tgggctgggc acgatgggtt ttggcctgcc tgcggcgatt
     5101 ggcgcggcgc tggcgaaccc ggatcgcaaa gtgttgtgtt tctccggcga cggcagcctg
     5161 atgatgaata ttcaggagat ggcgaccgcc agtgaaaatc agctggatgt caaaatcatt
     5221 ctgatgaaca acgaagcgct ggggctggtg catcagcaac agagtctgtt ctacgagcaa
     5281 ggcgtttttg ccgccaccta tccgggcaaa atcaacttta tgcagattgc cgccggattc
     5341 ggcctcgaaa cctgtgattt gaataacgaa gccgatccgc aggctgcatt gcaggatatc
     5401 atcaatcgcc ctggcccggc gctgatccat gtgcgcattg atgccgaaga aaaagtttac
     5461 ccgatggtgc cgccaggtgc ggcgaatact gaaatggtgg gggaataagc catgcaaaac
     5521 acaactcata acaacgtaat tctggagctc accgttcgca accatccggg cgtaatgacc
     5581 cacgtttgtg gcctttttgc ccgccgcgct tttaacgttg aaggcattct ttgtctgccg
     5641 attcaggaca gcgacaaaag ccatatctgg ctactggtca atgacgacca gcgtctggag
     5701 cagatgataa gccaaatcga taagctggaa gatgttgtga aagtgcagcg taatcagtcc
     5761 gatccgacga tgtttaacaa gatcgcggtg ttttttcagt aaccgctcaa ggcttgaaca
     5821 acatcgcgct tatcgttaag gtaagcgcgt tttttttacc cgccaggaca agaccatgat
     5881 caccgttgcc cttatagacg atcacctcat cgtccgctcc ggctttgcgc agctgctggg
     5941 gctggaacct gatttgcagg tagttgccga gtttggttcg gggcgcgagg cgctggcggg
     6001 gctgccgggg cgcggtgtgc aggtgtgtat ttgcgatatc tccatgcccg atatctccgg
     6061 tctggagctg ctaagccagc tgccgaaagg tatggcgacg attatgctct ccgttcacga
     6121 cagtcctgcg ctggttgagc aggcgcttaa cgcgggggca cgcggctttc tttccaaacg
     6181 ctgtagcccg gatgaactca ttgctgcggt gcatacggtt gccacgggcg gctgttatct
     6241 gacgccggat attgccatta aactggcatc cggtcgtcag gacccgctaa ccaaacgtga
     6301 acgccaggtg gcggaaaaac tggcgcaagg aatggcggtg aaagagattg ccgccgaact
     6361 gggcttgtca ccgaaaacgg tacacgtcca tcgcgccaat ctgatggaaa aactgggcgt
     6421 cagtaacgac gtagagctgg cgcgccgcat gtttgatggc tggtgatgaa gacgttgttc
     6481 tcccgcttaa ttaccgttat tgcctgcttt tttatcttct ctgccgcatg gttttgcctg
     6541 tggagtatca gcctgcatct ggttgagcgc cctgatatgg cggtgctgtt atttccgttt
     6601 ggtctgcgtc tggggctaat gctgcaatgc ccgcgcggat actggccggt gctgctgggc
     6661 gcggagtggc tgctgattta ctggctaacg caggcggtcg gtttaaccca ttttccgtta
     6721 ttgatgatcg gtagtttact gacgttactg cccgtagcgc tgatctcgcg ctatcgccat
     6781 cagcgtgact ggcgcacctt gctgttacag ggggcggcgt taacggcggc ggcgttgttg
     6841 caatcgctgc cctggctttg gcacggcaaa gagtcgtgga atgcgctgtt gctgacttta
     6901 actggcggcc tgacgctggc cccgatatgt ctggtgttct ggcactatct cgccaataac
     6961 acctggctgc cgctcggccc gtcactggtt tctcagccaa tcaactggcg cgggcgacat
     7021 ctggtctggt acttgctgct gtttgttatc agtctctggc tccagttggg attgccggac
     7081 gaactgtcgc gctttacgcc attctgtctg gcgctgccga ttatcgcgct ggcctggcac
     7141 tatggttggc aaggggcgct gattgcgacg ttgatgaacg ccatcgcgct gatcgccagt
     7201 caaacctggc gcgatcatcc ggtggattta ttgctctcgc tgctggtgca aagtctgaca
     7261 gggttgttgc ttggcgctgg catccagcgg ttgcgtgaac ttaaccagtc gctgcaaaag
     7321 gaactggcgc gcaatcagca tctggctgaa cggttgctgg aaaccgaaga gagcgtgcgc
     7381 cgtgatgtgg cgcgtgagct gcatgatgat atcggtcaga ccatcactgc tattcgtact
     7441 caggcgggca ttgttcagcg gctggcggca gataacgcca gcgtgaagca gagcgggcag
     7501 ctcatcgaac aactatcgct gggcgtttac gacgcggtgc gccgtttgtt gggtcggtta
     7561 cgtccgcgcc agttggatga tctcaccctg gagcaggcca tccgctcact gatgcgggaa
     7621 atggagctgg aagggcgcgg tattgtcagc catctcgaat ggcgaatcga tgaatcagcg
     7681 ttaagcgaaa accagcgcgt gacgctgttt cgtgtctgcc aggaagggct gaacaacatt
     7741 gtgaaacatg ctgatgccag cgcggtcacc ctgcaaggct ggcagcagga tgaacggttg
     7801 atgctggtta ttgaagacga tggcagcggt ttgccgccgg gttccgggca acaaggtttt
     7861 ggcctcaccg gaatgcgcga gcgcgtaacg gcgctgggtg gcacattaca catttcctgt
     7921 ctgcacggca cgcgtgtcag cgtttctcta cctcaacgct atgtctaagg tttgatgatg
     7981 ttgccgtttc tgaaagcgcc tgccgatgcg ccattaatga ctgataaata tgaaattgat
     8041 gcccgctatc gctactggcg tcggcatatt ctgctgacca tctggctggg ttacgcgctg
     8101 ttttacttca cgcggaaaag ttttaacgcc gccgtaccag aaatccttgc taacggcgtg
     8161 ctcagccgta gcgatatcgg cctgttagcg accctgtttt acattaccta tggcgtgtcg
     8221 aagtttgtct ccggcattgt cagcgatcgc tcaaatgccc gttattttat ggggataggg
     8281 cttatcgcca cgggcattat caacattctg tttggcttct cgacgtcgct atgggcgttt
     8341 gccgtactct gggtgctgaa cgcctttttc cagggctggg gttcaccggt gtgtgcgcgt
     8401 ctgttaacgg cctggtattc acgtaccgag cgcggtggtt ggtgggcatt atggaacacg
     8461 gcgcataacg tcggcggcgc actcattccc attgtgatgg cagcggctgc actacattac
     8521 ggctggcgtg ccgggatgat gattgctggt tgtatggcga tcgtcgtggg aatttttctc
     8581 tgctggcggc tgcgcgatcg cccgcaggcg ttaggtttac cggcggtcgg tgaatggcga
     8641 cacgacgcgc tggagatagc tcaacaacaa gaaggggcag ggctgacgcg taaagagatc
     8701 ctcaccaaat atgtgttgct gaatccgtat atctggctgc tttcgttttg ctatgtgctg
     8761 gtctatgtgg tccgggcggc gatcaacgac tggggcaatt tgtatatgtc cgagacgctg
     8821 ggcgtcgatc tggtcacggc gaatacggca gtgacgatgt ttgaactggg tggatttatc
     8881 ggtgcgctgg tagcgggttg gggctcggac aaattgttta acggcaaccg agggccgatg
     8941 aatttgattt tcgccgccgg aattttgctt tcagtcggct ccctgtggct gatgccattt
     9001 gccagctacg tgatgcaggc aacctgcttc ttcaccattg gtttttttgt ctttggccca
     9061 cagatgttaa tcggtatggc ggcggcagag tgttcccaca aagaggcggc aggggcggcg
     9121 acggggtttg tcggcttgtt tgcttatctg ggggcgtcgc ttgctggttg gccgctggcg
     9181 aaagtactcg atacctggca ctggagcgga ttttttgtgg ttatctctat cgccgccggg
     9241 atttccgcac tgctgttact gccctttttg aacgcccaga caccgcgcga agcgtgatgc
     9301 atctcacctt ttcacttcat atccggcaaa actaagaaat tttccaggtt ttgcctggac
     9361 gctatctcag gcctgatttg ctgctgattt ttacaatgca tgcctcacgc aggtattcat
     9421 ttcaggagta acccatgctg gctttcctaa accaggttcg caagccgacc ctggaccttc
     9481 cgctcgaagt gcggcgcaaa atgtggttca aaccgttcat gcaatcctac ctggtggtct
     9541 ttatcggcta cctgacgatg tacctgattc gcaagaactt taacatcgcg cagaacgata
     9601 tgatttcgac ctacgggttg agcatgacgc agctggggat gatcggcctg ggtttctcca
     9661 tcacttatgg cgtgggtaaa acgttggttt catactacgc cgacggcaaa aacaccaaac
     9721 aattcctgcc gttcatgctg atcctgtctg ccatttgtat gctgggcttc agtgccagta
     9781 tgggcagcgg ctcggttagc ctgttcctga tgattgcctt ctacgcctta agcggctttt
     9841 tccagagtac cggcggttcg tgcagttact ccaccatcac caaatggacg ccgcgtcgta
     9901 aacgcgggac attcctcggt ttctggaata tttctcacaa ccttggcggt gcaggcgcag
     9961 caggtgtggc gctgttcggg gcaaattacc tgttcgatgg ccatgtcatc ggcatgttta
    10021 tcttcccgtc gattatcgcg ctgattgtcg gttttatcgg cctgcgttac ggcagcgact
    10081 ccccagaatc ttatggcctc ggcaaagctg aagaactgtt cggcgaggag atcagcgaag
    10141 aggacaaaga gacagaatct accgatatga ccaagtggca gatctttgtt gagtatgtgc
    10201 tgaaaaacaa agtgatctgg ctgctgtgct tcgccaacat t
//
LOCUS       DAJTYD010000138        10213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTYD010000138 DAJTYD010000000
VERSION     DAJTYD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.138"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            141..1997
                     /gene="kup"
                     /locus_tag="OO585_003086"
     CDS             141..1997
                     /gene="kup"
                     /locus_tag="OO585_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6630590.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2164..2583
                     /gene="rbsD"
                     /locus_tag="OO585_003087"
     CDS             2164..2583
                     /gene="rbsD"
                     /locus_tag="OO585_003087"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6630591.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2591..4060
                     /gene="rbsA"
                     /locus_tag="OO585_003088"
                     /pseudo
     CDS             2591..4060
                     /gene="rbsA"
                     /locus_tag="OO585_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4065..5030
                     /gene="rbsC"
                     /locus_tag="OO585_003089"
     CDS             4065..5030
                     /gene="rbsC"
                     /locus_tag="OO585_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6630592.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5055..5941
                     /gene="rbsB"
                     /locus_tag="OO585_003090"
                     /pseudo
     CDS             5055..5941
                     /gene="rbsB"
                     /locus_tag="OO585_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6067..6996
                     /gene="rbsK"
                     /locus_tag="OO585_003091"
     CDS             6067..6996
                     /gene="rbsK"
                     /locus_tag="OO585_003091"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6630593.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7000..7992
                     /gene="rbsR"
                     /locus_tag="OO585_003092"
     CDS             7000..7992
                     /gene="rbsR"
                     /locus_tag="OO585_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6630594.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO585_003093"
     CDS             complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO585_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6630595.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTAQRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9408..10100)
                     /locus_tag="OO585_003094"
     CDS             complement(9408..10100)
                     /locus_tag="OO585_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6630596.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2486 a         2458 c         2702 g         2567 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtgct gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag
     1681 acgcgccata tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgaaacg cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta
     1921 atgcgctgcg tgcaccagat caatttgaaa tcccgccaaa cagggttatc gaactgggta
     1981 ctcaggtcga aatctaacgc cagacgcctc ctttctccat aagggggcgt ttttgttttc
     2041 atggttaatc accatgtaaa acgtttcgag gttgatcaca tttccgtaac gtcacgatgg
     2101 ttttcccaac tcagtcagga ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa
     2161 aaaatgaaaa aaggcaccgt tcttaattct gatatttcat cggtgatctc ccgtctggga
     2221 cataccgata cgctggtggt gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt
     2281 atcgatatgg cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgttaca
     2341 aatgaaatgc aggtcgaggc tgccattatc gcggaagaga tcaaacaaca taatccgcaa
     2401 ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt
     2461 gaaattcgtt acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta
     2521 attcgcagcg gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc
     2581 tgaggccgtc atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt
     2641 aaaagccctc tcgggcgctg cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg
     2701 cgaaaacggc gcgggtaaat ccaccatgat gaaagtgctt actggtatct atgctcgcga
     2761 tgccggcacg cttttatggc tggggaaaga aacgacattt accgggccga aatcttccca
     2821 ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt tgaccattgc
     2881 cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac
     2941 catgtatgcc gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa
     3001 gctggtgggc gatctttcca tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag
     3061 ctttgagtcg aaagtcatca ttatggatga accgaccgat gcgctgaccg ataccgaaac
     3121 cgaatccctg ttccgcgtca tccgcgaact aaaatcgcaa ggccgcggta ttgtctatat
     3181 ctcccaccgc atgaaagaaa tcttcgagat ttgcgatgcc gttaccgttt ttcgtgacgg
     3241 gcaatttatt gctgagcgcg aagtggcatc actgaccgaa gattcgctga ttgagatgat
     3301 ggtgggtcgc aagctggaag atcaatatcc acacctggac aaagcgccgg gagatatccg
     3361 cctgaaagtc gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa
     3421 aggcgaaatt cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt
     3481 gctctacggc gcactaccgc gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt
     3541 tacccgttca ccgcaggatg gcctggcaaa cggcattgtg tatatctccg aagaccgtaa
     3601 acgtgacggt ttagtgttgg gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg
     3661 ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat gaacagtagg ctgtgagtga
     3721 tttcattcgt ctgtttaatg tgaaaacacc gtcgatggaa caggcaattg gtctgctttc
     3781 cggtggcaat cagcaaaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg
     3841 cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat
     3901 tctggtgtca tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca
     3961 tgaagggcat ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc
     4021 tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg
     4081 tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc
     4141 tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact
     4201 tattcaatat tctccagcaa acctcggtga acgccattat ggcggtcggg atgacgctgg
     4261 tgatcctgac ttcgggcatc gacttgtctg taggttctct gttggcgcta accggcgcag
     4321 ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gctgctctcg
     4381 cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt cgcgttcagg
     4441 cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca
     4501 acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta
     4561 ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg
     4621 cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg
     4681 aagcggcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc
     4741 tttgtggtct gctggcatcg ctggccggga ccattgaagt ggcgcgtctc tcctccgcac
     4801 aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta
     4861 cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg
     4921 gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca
     4981 aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac
     5041 aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa
     5101 gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg gtctccacgc
     5161 ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataagcttg
     5221 gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc
     5281 aggacttaac cgttcgcggc accaaaattc tgctgattaa cccgaccgac tccgacgcag
     5341 taggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc
     5401 aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa
     5461 tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag
     5521 gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg
     5581 ctcacaagtt taatgttctt gccagccagc agattttgat cgcactaaag gtttgaacgt
     5641 aatgcagaac ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga
     5701 aatggcgctg ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt
     5761 cggatttgac ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac
     5821 tatcgctcag ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct
     5881 gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta
     5941 gttttaatca ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg
     6001 taatataaag aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc
     6061 ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt
     6121 cttaatcttc aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt
     6181 gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc
     6241 gcgtttattg cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc
     6301 actgataaca ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg
     6361 ctgatttttg ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct
     6421 gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta
     6481 ttaatgcagc tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa
     6541 aataagacta tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg
     6601 gcgctggtgg acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt
     6661 gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt
     6721 actgtactga ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag
     6781 cgcgttcctg gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac
     6841 ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc
     6901 catgctgcag ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt
     6961 gaagagatcg acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt
     7021 gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc
     7081 gtcagtgaag cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca
     7141 tcagccctgg cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact
     7201 gccagtacca atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa
     7261 cgcggttata gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat
     7321 ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat
     7381 caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg
     7441 gctccgttcg atggcgacag cgatcttatt caggataact cgttgctggg cggagactta
     7501 gcaacgcaat atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg
     7561 gataaaactc cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt
     7621 ctcaacattc ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac
     7681 gccatgcgcc aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac
     7741 gctatggctg ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat
     7801 atcgcggtga ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc
     7861 actatccacc aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg
     7921 ataacccagc cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc
     7981 ggttcggctt agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa
     8041 gcatgaacat tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga
     8101 attgttcgac agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta
     8161 cagcaacgcc taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc
     8221 tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg
     8281 acatagccat ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg
     8341 attgcaaaga gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat
     8401 ggcgatagcc cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta
     8461 acgctgtcgg tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta
     8521 acatcaatgg cataaggaac ggtacactac cggtccccag acgggttgca atattgccta
     8581 cgataccgat cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg
     8641 tgtgtcgcgc atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca
     8701 aggcaatcca gctggcgaca atcttttccc cgaatagctc tattccgctt gagaagagaa
     8761 caaggctgag gccaaacagc aaaaagccag tgatatcgaa tctgcgttgt gcggtggtga
     8821 aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta
     8881 aaaatatcca gtgccaggtt gccctggtga ccagcacgcc gccaagaacg gggcctaaaa
     8941 ttggccccac cagacccggc atggcgacaa aattcaatac aggaagaagt tcattacgag
     9001 gataagcacg cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct
     9061 gaataacccg gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac
     9121 ccaacgtgaa cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta
     9181 gccatccgct taccggaata aacatcgcca ccgtcagcgt ataactgatg atggctgatt
     9241 gcatcgcgag aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa
     9301 tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca
     9361 tactacgctt ctttttatcg ctcattcaat gtcctgttat cgggttatca cttatcaggt
     9421 gagcgtagca gcgcctgaca agttttaaat gccgcgtcgc catcgctttg gataatcgca
     9481 tcgacaatcg cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag
     9541 taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc
     9601 atttcataga tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag
     9661 ttctttttta atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg
     9721 gtgccaaccg ttgctgccag taggcaggct tgcggctcca gacagatacg cataaccaga
     9781 aagtgatcga tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga
     9841 tcaagaaaat tccagtttga ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa
     9901 accatccctt ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca
     9961 aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt
    10021 aagatgcgtt gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac
    10081 tgttgtgcgc ttaaaggcat tactaatctt cctttttctt tttattcctc cttagtatgc
    10141 caccaggaag tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa
    10201 cggggctttt gct
//
LOCUS       DAJTYD010000139        10212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTYD010000139 DAJTYD010000000
VERSION     DAJTYD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.139"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(272..1303)
                     /gene="rsmC"
                     /locus_tag="OO585_003095"
     CDS             complement(272..1303)
                     /gene="rsmC"
                     /locus_tag="OO585_003095"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6630597.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1406..1819
                     /gene="holD"
                     /locus_tag="OO585_003096"
     CDS             1406..1819
                     /gene="holD"
                     /locus_tag="OO585_003096"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6630598.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1788..2234
                     /gene="rimI"
                     /locus_tag="OO585_003097"
     CDS             1788..2234
                     /gene="rimI"
                     /locus_tag="OO585_003097"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6630599.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2249..2926
                     /gene="yjjG"
                     /locus_tag="OO585_003098"
     CDS             2249..2926
                     /gene="yjjG"
                     /locus_tag="OO585_003098"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6630600.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3017..4606
                     /gene="prfC"
                     /locus_tag="OO585_003099"
     CDS             3017..4606
                     /gene="prfC"
                     /locus_tag="OO585_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6630601.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4999..5604
                     /gene="osmY"
                     /locus_tag="OO585_003100"
                     /pseudo
     CDS             4999..5604
                     /gene="osmY"
                     /locus_tag="OO585_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            5731..5892
                     /locus_tag="OO585_003101"
     CDS             5731..5892
                     /locus_tag="OO585_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6630602.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6014..7087
                     /locus_tag="OO585_003102"
     CDS             6014..7087
                     /locus_tag="OO585_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6630603.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            7084..7866
                     /locus_tag="OO585_003103"
     CDS             7084..7866
                     /locus_tag="OO585_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6630604.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8080..8943)
                     /locus_tag="OO585_003104"
     CDS             complement(8080..8943)
                     /locus_tag="OO585_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6630605.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVGSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8915..>10212)
                     /locus_tag="OO585_003105"
     CDS             complement(8915..>10212)
                     /locus_tag="OO585_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6630606.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
BASE COUNT         2412 a         2670 c         2750 g         2380 t
ORIGIN      
        1 tcgcatacca tcaattctta aaaagaattg ctaccacgga ggcgcattct agtggttttc
       61 agcttttcgt caatagttaa ttatcgccgg aggtgtaatt gctggaaaaa tgtccatcag
      121 gaaactagcg tgcaggtttg gtaggcatgc gggggcagat gccagatgcg acgctggcgc
      181 gtcttatctg gcctacgaag ggctaacgtg caggttttgt aggtcggata aggcgtttac
      241 gccgcatcca gcaaggcttg cggcgggata cttaaccttt tttcgcctgg cgggtcataa
      301 tggcgcgata taccttgaaa cgcccggttt gcgcaatcac ttcatggaag ccaaatgttt
      361 catccagcac gtccggataa ggcaggaagg cgttcgctac aattcgcagc tcgccgccac
      421 tattaagatg acgcaccgcg ccgcgaatca gcgtttgcgc cgcatccagg ctggtttgca
      481 tcccatcgtg gaacggcggg ttggagatga tcatatcaaa acgacctttc acctcggaaa
      541 agacgttgct ggcaaagact tcaccttcaa cacagttggc cgcaagtgtt gcgcggctgg
      601 cttctaccgc tggcgcagag acatcgcaca aggtgagacg aattttcggc gaatggcgcg
      661 caaaggcaac tgaaagcacc cccgcgccac agccgacatc cagcacttta cctttcgtgt
      721 gcggagttaa cgtcgagagc agcaactggc taccgacatc cagaccgtcg cggctaaaca
      781 cgccaggcag cgttttgacc gtcaggccat cgacgctgta ttcgccccag aatttatcgg
      841 catcaaatac cggctgtttt tccagacgac caaaatagag gccacagcga cgagcgctgt
      901 cgactttatt caacggcgca taatctgcca gcatctgctc ggcgctgcgc acgccgctgc
      961 ggttctcgcc aacgacaaaa atatctgtcc ccactggcag cagagaaagt aaattcatca
     1021 actggaactg ggcttccggt ttgttcttcg gccagtagta aatcagcgta tcgcaatctg
     1081 cgacgtcatc caccgtggcg accagactga aacgggcgtt atcccccatc tggcggctta
     1141 atacctgcca gtggtggaat tgctgggtat gagcacggct ggccgcggta tctaaacgcg
     1201 cgggcaggtc atcctgtaag tctccggcaa acagaatacg gctttgttcg aaatcatcac
     1261 tgtgacgcag caagacttca cttgccgggg taaatgcaga catggaatgc tcctcaattg
     1321 atactggcgg cgattatagc catatgttgg cgcggtatcg acgaatttgc tatatttgcg
     1381 cccctgacaa caggagcgat tcgctatgac atcccgacga gactggcagt tacagcaact
     1441 gggcattacc cagtggtcgc tgcgtcgccc tggcgcgttg cagggggaga ttgccattgc
     1501 gatcccggca cacgtccgtc tggtgatggt ggcaaacgat cttcccgccc tgaccgatcc
     1561 tttagtgagc gatgttctgc gcgcattaac cgtcagcccc gatcaagtgc tgcaactgac
     1621 gccagaaaaa atcgcgatgc tgccgcaagg cagtcgctgc aacagctggc ggttgggtac
     1681 tgacgaaccg ctatcactgg aaggcgctca ggtggcatca ccggcgctca ccgaattacg
     1741 ggcaaaccca acggcacgcg ccgcgttatg gcaacagatt tgcacatatg aacacgattt
     1801 cttccctcga aacgactgat ttaccagcgg cttaccacat tgaacaacgc gcccacgcct
     1861 ttccgtggag tgaaaaaaac tttgccagca accagggcga gcgttatctc aactttcagt
     1921 taacgcaaaa cggcaaaatg gcggcgtttg cgattacgca agtggtgctg gatgaagcta
     1981 cattgttcaa tattgtggtc gatcctgact atcagcgtca gggattggga agggtgctgc
     2041 tgaaacatct gatcgacgaa ctggaaaaac gcggcgtggc gacactatgg ctggaagtcc
     2101 gtgcttcaaa cgctgccgcc attgccctgt acgaaagttt aggctttaac gaggcgacga
     2161 ttcgccgcaa ttactacccc accacggacg gtcgcgaaga cgccatcatc atggcgttgc
     2221 caatcagtat gtaagacaag gtggaataat gaagtgggac tggattttct ttgatgccga
     2281 tgaaacgctg tttacctttg actcattcac cggcctgcag cggatgtttc ttgattacag
     2341 cgtcaccttt accgctgaag attttcagga ctatcaggcc gttaacaagc cactgtgggt
     2401 ggattatcaa aacggcgcga tcacttcatt acagcttcag cacgggcgtt tcgagagctg
     2461 ggccgaacgg ctgaaagttg aagcaggctt gcttaacgat gcctttatta atgcgatggc
     2521 ggaaatctgc acgccgctgc cgggcgcggt ttctctgctt aacgccattc gtggcaacgc
     2581 taaaatcggc atcatcacca acggttttag cgccttgcag caagtgcgtc tggaacggac
     2641 gggcctgcgt gattactttg atttgctggt gatttccgaa gaagttggca ttgccaaacc
     2701 gaataagaaa attttcgatt atgcgctgga actggcgggc aatcctgacc gttcacgcgt
     2761 gctgatggtt ggcgacactg ccgagtccga tattctcggt ggcatcaacg ccgggcttgc
     2821 gacttgctgg ctgaatgcgc accatctcga gcaaccagaa ggcatcgcgc ccacctggac
     2881 cgtttcatcg ttgcacgaac tggagcagct cctgtgtaaa cactgattgc ctcccccccg
     2941 ttgatgggta aaatagccgc aatttttcgt tttcaacaag cgcggcgcga tgccgcttac
     3001 tcaagaagaa agaattatga cgttgtctcc ttatttgcaa gaggtggcga agcgccgcac
     3061 ttttgccatt atttctcacc cggacgccgg taaaactacc atcaccgaga aggtgctgct
     3121 gttcggacag gccattcaga ccgctggtac agtaaaaggc cgtggttcca accagcacgc
     3181 taagtcggac tggatggaga tggaaaagca gcgtgggatc tccattacta cgtctgtgat
     3241 gcagtttccg tatcacgatt gcctggttaa cctgctagac accccggggc acgaagactt
     3301 ctcggaagat acctatcgta ccctgacagc ggtggactgc tgcctgatgg ttatcgacgc
     3361 tgcaaaaggt gttgaagatc gtacccgtaa gctgatggaa gttacccgtc tgcgcgacac
     3421 gccgatcctc acctttatga acaaacttga ccgtgatatc cgcgacccga tggaactgct
     3481 cgatgaagtt gagaacgagc tgaaaatcgg ctgcgcaccg atcacctggc cgattggctg
     3541 cggcaagctg tttaaaggcg tttaccacct ttataaagac gaaacctatc tttatcagag
     3601 cggtaaaggc cacacgattc aggaagtccg tattgttaaa gggctgaata acccggatct
     3661 cgacgctgcg gttggtgaag atctggcaca gcagctacgt gacgaactgg aactggtgaa
     3721 aggcgcgtct aacgagttcg acaaagaact gttccttgcg ggcgaaatca ctccggtatt
     3781 cttcggtact gcgctgggta acttcggcgt tgatcatatg ctggatggcc tggtggagtg
     3841 ggcccctgcg ccgatgccgc gtcagactga tacccgtacc gtagaggcga gcgaagacaa
     3901 atttaccggc ttcgtattta aaattcaggc caatatggac ccgaaacatc gcgaccgcgt
     3961 ggcgtttatg cgcgtagtat ccggtaaata tgaaaaaggc atgaagctgc gccaggtgcg
     4021 tactgcgaaa gatgtggtta tctccgacgc gctgaccttt atggcgggcg accgttcgca
     4081 cgttgaagaa gcgtatccgg gcgatatcct cggcctgcac aaccacggca ctattcagat
     4141 cggcgacacc tttacccagg gtgagatgat gaagttcacc ggtattccga acttcgcacc
     4201 agaactgttc cgtcgtatcc gcctgaaaga tccgctgaag caaaaacagc tgctcaaagg
     4261 gctggtacag ctttccgaag agggcgcggt gcaggtgttc cgtccgatct ccaacaacga
     4321 tttgatcgtt ggtgctgttg gtgtgctgca gtttgatgtg gtggtatcgc gcctgaagag
     4381 cgaatacaac gttgaagcag tatatgaatc agtcaacgtt gccactgccc gctgggtaga
     4441 atgtgcggac gcgaagaaat tcgaagagtt caagcgtaag aacgaaagcc aactggcgct
     4501 tgatggcggc gataacctcg cttacatcgc taccagcatg gtcaacctgc gcctggcgca
     4561 ggaacgttat cccgacgttc agttccacca gacccgcgag cattaattct tgcctccagg
     4621 gcgcggtagc cgctgcgccc tgtcaatttc ccttccttat tagccgctta cggaatgttc
     4681 ttaaaacatt cacttttgct tatgttttcg ctgatatcac gagcggtttc aaaattgtga
     4741 tctatattta acaaagtaat gacatttctg acggcgttaa ataccgttca atgcgtagat
     4801 atcagtatct aaagccgtcg attgtcattc taccgatatt aataactgat tcagaggctg
     4861 taatggtcgt tattcatcac ccatcgcttt tgtgatggcg gccattgact tctgtagagg
     4921 gtgaagtctc tccctattca gcaatgcaac ctcgtgttgc caggctcaaa ttacgagcaa
     4981 acatacagga ataaatcgat gactatgaca agactgaaga tttcgaaaac tctgctggct
     5041 gtaatgttga cctctgccgt cgcgaccggc tctgcctacg cggaaaacaa cgcacagact
     5101 accaatgaaa gcgcagggca aaaagtcgat agctctatga ataaagtcgg taatttcatg
     5161 gatgacagcg ccatcaccgc gaaagtgaag gcggcgctgg tggatcatga caacatcaag
     5221 agcaccgata tctctgtcaa aaccgagcaa aaagtcgtga ccctgagcgg tttcgttgaa
     5281 agctaggctc aggccgaaga ggcagtgaaa gtggcgaaag gcgttgaagg ggtgacctct
     5341 gtcagcgaca aactgcacgt tcgcgacgct aaagaaggct cggtgaaggg ctacgcgggt
     5401 gacaccgcca ccaccagtga aatcaaagcc aaactgctgg cggacgatat cgtcccttcc
     5461 cgtcatgtga aagttgaaac caccgacggc gtggttcagc tctccggtac cgtagattct
     5521 caggcacaaa gtgaccgtgc tgaaagtatc gccaaagcgg tagatggtgt gaaaagcgtt
     5581 aaaaatgatc tgaaaactaa gtaattcgtc gtaattcgtc ctcccgaact ttgtcgggag
     5641 gcgcgatgtg caccacacta aaaatatcgc attgagtgat tttcacgctc atatcaagcg
     5701 gtgacattaa ctatggtaaa ggagacgctt atgtttcgtt ggggcatcat atttctggtt
     5761 atcgcgttaa tcgccgccgc acttgggttt ggtggtttgg ccggtaccgc tgcaggcgca
     5821 gctaaaattg tctttgtcgt cgggattatt ctgttcctgg tgagtttgtt catgggccga
     5881 aaacgaccct agatttcaga ccattaagaa aattttatcc aaagccagtc cagcggactg
     5941 gcttttgcgg ttttagcgaa taataaattg cgttactttg tcattctttc acaacaaaaa
     6001 caggaaggca gaggtggggc agcgaatacc tgtaacgctt ggtaatattg cgccgttgtc
     6061 gctaaggccg ttccagcctg gacgaatagc tctggtgtgc gaaggcggcg gacagcgtgg
     6121 aattttcacg gctggcgtgc tggatgagtt tatgcgcgcg cagtttaatc ctttcgatct
     6181 ttatctcggc acatctgccg gggcgcagaa cctctcggcg tttatctgca atcagcccgg
     6241 ttacgcgcgt aaagtcatca tgcgctatac cacaaaacgc gaatttttcg acccattgcg
     6301 ctttgtccgt ggaggaaatc ttatcgatct cgactggctg gtggaggcca ccgcaagcca
     6361 gatgccgttg caaatggata ccgccgcgcg gttgtttgac agcggcaaat cgttttatat
     6421 gtgcgcctgt cgtcaggatg actacgcgcc taattacttt ttaccaacca aacaaaactg
     6481 gctggatgtg attcgcgcct ccagtgcgat acctggcttt tatcgtagcg gagtgtcgct
     6541 ggaaggcatt aactacctgg atggcgggat cagtgatgcg attccggtta aagaggcggc
     6601 aaggcagggc gctaaaacgt tggtcgtcat tcgcactgtg ccgtcacaaa tgtactacac
     6661 gccgcagtgg ttcaaacgca tggaacgctg gctgggtgac agtagcctgc agccgctggt
     6721 caatctggtg cagcatcatg aaaccagcta tcgtgacatt cagcaattta ttgagaaacc
     6781 accgggcaag ctgcggatat tcgaaattta tccgccgaag ccattacata gtatcgcgct
     6841 tggcagtcgg attccggcgc tgcgtgaaga ctataaactt gggcgtttat gcggttgtta
     6901 tttcctcgcc acggttggca agctattaac tgaaaaagcg ccgcttaccc gccatctggt
     6961 gccagtggtg acgccggaat cgatcgttat tccgcctgcg ccagtcgcca acgatacgct
     7021 ggttgccgaa gtgattgacg ctccgcaggc gaacgacccg acatttaaca atgaggatct
     7081 ggcttgattt gccgttttat cgacacccac tgccattttg atttcctgcc gtttagtggc
     7141 gatgaagagg ccagcctgca acgcgcggca caagcgggcg taggcaagat cattgttccg
     7201 gcgacagagg cggaaaattt tgcccgtgtg caggcgttag cggaaaaata tcaaccgctg
     7261 tatgccgcat tgggcttgca tccaggtatg ttggaaaaac atagcgatgt gtctcttgag
     7321 cagctacagc aggcgctgga aaggcgtccg gcgaaggtgg tggcagtggg ggaggtcggt
     7381 ctggatctct tcggcgacga tccgcaactt gagaggcagc agtggttact cgacgaacag
     7441 ctgaaactgg cgaaacgcta cgatctgccg gtgatcctgc attcacggcg cacacacgac
     7501 aaactggcga tgcatcttaa acgccacgat ttatcgcgca ctggcgtggt tcacggtttt
     7561 tccggcagcc tgcaacaggc cgagcggttt gtacagctgg gctacaaaat cggcgtaggc
     7621 ggtactatca cctatccacg cgccagtaaa acccgcgatg tcatcgcaaa attaccgctg
     7681 gcatcgttgt tgctggaaac cgacgcgccg gatatgccgc tcaacggctt tcaggggcag
     7741 cctaaccgac cggaacaggc tgtgcgggtg tttgacgtgc tttgcgagtt gcgcccggaa
     7801 ccagaagatg agattgccga agtgttgctt aataatacgt atgcgttgtt tagcgttagt
     7861 gggtagttta cccttcgtag gcagtatccg gcagtcggcg cataatgcct gatgcgacgc
     7921 ttgtcgcgtc ttatcatgcc tacaggtttg tgccgaaccg taggccggat aaggcgttca
     7981 cgccgcatcc ggcagttggc gcacaatgcc tgatgcgacg cttgtcgcgt cttatcatgc
     8041 ctacaagtct gtgccgaacc gtaggccgta tccggcatgt cacaaataga gcgccggaaa
     8101 tatcaaccgg ctcaccccgc gcacctttaa cgcatcagcc aacggctcaa cgtcttccgg
     8161 cgtggcgctc gcccagcttt gcgcctcgcc atacacgccg tgggcatgaa acgcattcag
     8221 gcgaaccgga acatcgccaa gtctcttgat aaacgtcgcc agctcatcga tgtgatgcaa
     8281 ataatccacc tggtctggaa ttaccagcaa acgcagttcc gccagcttgc cgcgctctgc
     8341 aagcaaacag atgctgcgct taatctgctg attatcgcgt ccggttaagt gttgatgaca
     8401 ttcgctcccc cacgctttga gatcgagcat tgcgccgtca cacaccggga gtaatttttc
     8461 ccagccggtt tcgctcaaca tgccgttact gcccaccagg caggtgaggt ggcgcagttg
     8521 cggatcgttt ttgatagcag taaacagcgc cactacaaac ggcagttggg tcgtggcttc
     8581 gccgccgctt accgttatcc cttcaataaa cagcaccgct ttgcggacat ggctaagcac
     8641 ttcgtccacg ctcatggatt gcgccattgg cgtggcatgt tgcggacaca tcttcaggca
     8701 ggtatcacac tgctcgcaga ccacagcgtt ccacagcact ttgccgtcaa caatctgcaa
     8761 cgcctgatgc ggacactgtg gcacgcactc tccgcaatca ttgcagcgtc ccatcgtcca
     8821 cggattgtga cagtttttgc agcgcagatt gcagccctgc agaaacagag ccagacgact
     8881 gcctggcccg tcaacgcagg agaaggggat aatcttactg actaaagcgc atctgctgtt
     8941 catggcttat cacgcgcggc tggcgttcca gaatacgagt gttgcgtgtg gcttcttcgc
     9001 ccagccaggt ggtgttggtg cgtgaacctt cggcgcgata tttttctaaa tccgacaaac
     9061 gcaccatata accggtaacg cgaaccagat cgttaccgct gacattggcg gtaaattcac
     9121 gcattccggc tttaaaggca ccgaggcaaa gctgtaccag cgcctgcgga ttacgtttga
     9181 tggtttcgtc gagcgtcaga atgtcgctga tgccggaata ataataagca tgatgcgggg
     9241 cgacagtttg cagatggatg atcggatctg gctcatcgcc atacggcaaa cgcgcgcccg
     9301 gcgtggtgcc gatatcggaa ctgatccccg actgtgcgtg taacatggcg cgtttttgcc
     9361 agccatattt cacgggggta ttggcgacaa actccgccag ttgcgcgctg atgcgataac
     9421 ccacttcatt tgcggcggct tctttaccgt agcgcgcggc aatcccttct ttctcacaca
     9481 gcaagttaac cgcttccgcc agcccataca tgccaaacat tggcacaaaa cgttcagggt
     9541 taatcagccc ttctttcacc aggaagctat tctcaaagaa gtgtgattgt tgatagagga
     9601 attcacaccg cgcatcgatg atggcgatct gctgctggca gtagtgcggt agagtgcgcg
     9661 taaagaagtc atccagcgat tcgctgcgct cggcaatggc tttcaggtta aggcgtacca
     9721 gcgtgctgcc accacccgcc agcggcagtg agttgtaaca gctcacaatc ccgtagcccc
     9781 cttttgtgaa aattttatca tgcaccggac cgttggcgat gtgcggtttg ctacattcac
     9841 agatgttctt cgccacttcc agcagcaggt catcaggggg gatttcagga tcgtagataa
     9901 aggtcaggtt cggtgaaacc tgcttcaact ctgcgtctgc acgtaagatc gcgcgggtaa
     9961 tgggcgaatc agacgggccg atattggcgt gcataaaggc gtctggcagg gttccgtcga
    10021 ggtaacgcca gaaacgtttt attcgaacat cgatctcgtc ttgtgttaga attctaacat
    10081 acggttgcaa caacgcatcc agttgcccta ggtagaccgg catcgatgtg accgacggta
    10141 cgtggtggta aagaatggtc agcagagaga gtgcgtcatc aagatctttc gcgccttcca
    10201 gctccagcca tt
//
LOCUS       DAJTYD010000140        10110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTYD010000140 DAJTYD010000000
VERSION     DAJTYD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.140"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OO585_003106"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OO585_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6630607.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OO585_003107"
     CDS             complement(1606..1929)
                     /locus_tag="OO585_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6630608.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO585_003108"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO585_003108"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6630609.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO585_003109"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO585_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6630610.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLHGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO585_003110"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO585_003110"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6630611.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO585_003111"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO585_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6630612.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OO585_003112"
     CDS             7529..8566
                     /locus_tag="OO585_003112"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6630613.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO585_003113"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO585_003113"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6630614.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OO585_003114"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OO585_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6630615.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..>10110)
                     /locus_tag="OO585_003115"
     CDS             complement(10047..>10110)
                     /locus_tag="OO585_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCR6630616.1"
                     /translation="RQLNIVLALLLVYCAVRIFY"
BASE COUNT         2500 a         2547 c         2614 g         2449 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgtataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccatgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga
//
LOCUS       DAJTYD010000141        10041 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTYD010000141 DAJTYD010000000
VERSION     DAJTYD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.141"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            205..357
                     /gene="hokA"
                     /locus_tag="OO585_003116"
     CDS             205..357
                     /gene="hokA"
                     /locus_tag="OO585_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6630617.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(545..757)
                     /gene="cspA"
                     /locus_tag="OO585_003117"
     CDS             complement(545..757)
                     /gene="cspA"
                     /locus_tag="OO585_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6630618.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1038..1328)
                     /locus_tag="OO585_003118"
     CDS             complement(1038..1328)
                     /locus_tag="OO585_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6630619.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1761..2471
                     /locus_tag="OO585_003119"
     CDS             1761..2471
                     /locus_tag="OO585_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6630620.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="OO585_003120"
     CDS             complement(2521..3495)
                     /gene="ghrB"
                     /locus_tag="OO585_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6630621.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3599..4258)
                     /locus_tag="OO585_003121"
     CDS             complement(3599..4258)
                     /locus_tag="OO585_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6630622.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4411..6744
                     /gene="bisC"
                     /locus_tag="OO585_003122"
     CDS             4411..6744
                     /gene="bisC"
                     /locus_tag="OO585_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6630623.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6713..7153)
                     /locus_tag="OO585_003123"
     CDS             complement(6713..7153)
                     /locus_tag="OO585_003123"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6630624.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7150..7713)
                     /gene="tag"
                     /locus_tag="OO585_003124"
     CDS             complement(7150..7713)
                     /gene="tag"
                     /locus_tag="OO585_003124"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6630625.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7871..8569
                     /locus_tag="OO585_003125"
     CDS             7871..8569
                     /locus_tag="OO585_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6630626.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8797..9999
                     /locus_tag="OO585_003126"
     CDS             8797..9999
                     /locus_tag="OO585_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6630627.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2397 a         2424 c         2586 g         2634 t
ORIGIN      
        1 ttttttagga ttatatcaag gagaagaaac aaacttatta agctagaata gctacgggtg
       61 cttgagactg tttgtctcag gtattcaccg aaaggcagac agagaaaagc cccacctgac
      121 tataaatcaa agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt
      181 ggaaatcaac acagggggct gggaatgccg caaaaatacg gattactttc attaatagtg
      241 atttgtttca cgcttttatt tttcacctgg atggtaagag attcactgtg tgaattgcat
      301 attaaacagg ggcgttatga gctggcggca tttttagcct gcaatttaaa agagtaagag
      361 tcttcggcgg gaaattattc ccgcctttct tacggcgttg cgcattctca ttgcacccac
      421 atttattctt cacaaaaata ataatagatt ttactacgcg atcgattatt tatttcctga
      481 aaaaaaataa aaaaaatccc cgccaaatgg cagggatctt agattctgtg cttttaagca
      541 gagattacag gctggttacg ttaccagctg ccgggccttt agcgccgctt tcgatggtga
      601 aggacacttt ctgaccttcg tccagagatt tgtaaccatc gttctggata gcagagaagt
      661 gtacgaacac atctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt
      721 tgaaccattt tacgatacca gtcattttac cggacatagt gtattacctt taataattaa
      781 gtgtgccttt cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac
      841 tacgaggggt atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt
      901 ctgtacgtca aaccgttgat gtgcattaag ccacgcattg gcgggtgatg caacaattat
      961 ttttcatatt tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca
     1021 ataaagtgga taaaagtcta ttccatcaac tgcttactta atgccgggtt ggcttgaatc
     1081 aaacgcatca attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt
     1141 accatggcga ctgatatgcc caaaacccgg gcgaaatcat cgatttttaa ccctgtccct
     1201 tttcgtaact gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc
     1261 gtttcatctt taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta
     1321 tattccattg aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt
     1381 aagaatagtc gggaaaagca gacacagcgg ggtgaagtca gggcatgctc tggctatgat
     1441 tcgattcagc cagtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat
     1501 atatctctta ccactcctgt tttgtaaaga tttcgaaaat ctaaagttct gcgaaggcgt
     1561 aaatctcggg ctacaaaaaa gcaacggcaa tatcattttt taacaatgaa acaacaaatt
     1621 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1681 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1741 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1801 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1861 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1921 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1981 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     2041 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2101 tgaacggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2161 ctgcgcactc tgcgctaaaa cagaccgatg atttgaaacc ggtctttgat caggcattca
     2221 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggtt gcacaaactt
     2281 tcacgcaaca actggtgatg gttggagact acattgctca acaaggtact caggtgagct
     2341 ttgtagcaaa tggtatccag ttcccgactt cgcagcaggc aagtgaatat aacaaactga
     2401 ttgcgccgtt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2461 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2521 ttagtccgcg acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2581 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2641 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2701 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2761 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg agggtttcat
     2821 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2881 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2941 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     3001 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3061 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3121 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3181 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3241 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3301 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3361 ttcggcaaaa attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg
     3421 aacggtgaaa tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3481 aacggacggc ttcatgcttc tctccattca ctgataaggc gctcacctta gcctgaaata
     3541 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3601 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3661 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3721 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3781 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3841 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc agggtcgcgc
     3901 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3961 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     4021 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4081 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagtccagac
     4141 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4201 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4261 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4321 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4381 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4441 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4501 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4561 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4621 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc acgtgagttg ggatgaggcg
     4681 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4741 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4801 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4861 gcggcacagg cgatcatgcc gtatgtcgtg ggtggcagcg aagtttatca acagcaaacc
     4921 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4981 aatacgctga aaattgcgtg gaatgcatct gatgagcagg ggctttctta cttttccgca
     5041 ctgcgtgaca gcgggaaaaa gctgatctgt attgatccaa tgcgatcgga aaccgtcgat
     5101 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5161 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt
     5221 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcc
     5281 aaaaccgccg aatgggcggc agggatttgt ggggttcacg cagcgaaaat tcgcgagttg
     5341 gcggctcttt tccaccaaaa cacgaccatg ctgatggctg gctggggaat gcagcgccaa
     5401 cagcttggtg agcaaaaaca ctggatgatc gtcacgctgg cggcaatgtt ggggcaaatc
     5461 ggcacacccg gcggcggttt tggtctttct tatcattttg ccaatggtgg taatcccacg
     5521 cggtgttctg cggtgctctc ttccatgcag ggtagcttgc cgggtggcag cgatgcggta
     5581 gataaaatcc ctgttgcccg tattgttgaa gcactggaaa accctggctg cgcataccaa
     5641 cacaatggca tgaatcgcct cttcccggat attcgcttta tctggtgggc gggtggcgcc
     5701 aacttcaccc atcatcagga cactaatcgc ctgatacgag cttggcaaaa accggagctg
     5761 gtagttatct ccgaatgctt ctggacggcg gcggcaaaac acgcggatat cgttctgcct
     5821 gcgactacct cttttgagcg taatgatctc accatgaccg gcgattacag taatcagcat
     5881 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5941 tttgccgagt taagtgaacg ctgggagaag ggcggttatg cacgttttac ggaaggaaaa
     6001 agtgagttgc aatggctgga aacgttttat aacgttgccc gacagcgcgg ggcaagccag
     6061 caggttgaat tgccgccatt tgctgagttc tggcaagcca atcagttaat tgagatgccg
     6121 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgcaggcg
     6181 catccattaa aaaccgccag cggtaagatt gaaatcttct cgcagcgtat tgccgattac
     6241 gcttatccgg attgcccggg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6301 gccgaaccgg aacagttaca ggtactttct gctcatccgg cgcatcgtct gcacagccag
     6361 ctgaattaca gttctctgcg cgaattgtat gcggtggcaa atcgtgagcc tgtcaccatt
     6421 catcctgacg atgccctggc gcgcggcatt caagatggcg atactgttcg gctgtggaac
     6481 gcacgcgggc aaattcttgc cggagtggtc attagcgagg gaattaaacc tggcgtgatt
     6541 tgtattcacg aaggggcatg gccggatttg gatttaaccg ctgacggtat ttgtaaaaac
     6601 ggcgcagtca acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6661 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6721 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6781 aatcgacaat gtgaaaaccc tgcgcctggt aaaaatttat cgccggttga tttttttgat
     6841 aaacctccag catcaggtgg ggatggcgct gctgcacata ttgcatcagc gccttaccaa
     6901 taccgcgcct gacggccttc ggtgcgacaa acatcgctgc cagaaatcgg ccttccataa
     6961 tgctgacaaa accgagaagc ttactgtctt cttcccagac ccagttttgc gcattggcaa
     7021 gatacgcatc ccgcaccagc ggaatgcaat cacgccagta attcgctttt ataaagggat
     7081 gtccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7141 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7201 cagcccacat gcctgcataa aggagtaaca gattgtggta ccgacaaact taaaaccacg
     7261 ttttttcagt gctttagata gggcgtcgga ggcgggcgta gatgtgggga tttcgctcaa
     7321 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcggcaaa
     7381 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7441 tttcccgcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7501 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7561 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7621 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7681 cgggtcctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7741 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7801 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7861 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7921 cgtggtaatc agcgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7981 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     8041 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8101 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8161 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8221 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8281 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8341 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8401 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8461 tatgttgctc aatcaaaata ttgccgccta tgccgcgcta actcaggcag aaaataccac
     8521 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8581 caattgaaac cattgaataa caatagttgt ggtgatagtg ggtgctaact tacaaataat
     8641 aaatttggtg aataattgtc gcgtcattca ttcctgaact aaggcatttc attccgttct
     8701 gatggcattt catgccgttt ttccccaggc ataaaatgca cttcgttatg gttgtcggca
     8761 gagatttttc ctttttatta ctgcaggaat actgccatga caccttcaaa ttatcagcgt
     8821 acccgctggc tgacactcat cggtactatc attacccagt ttgcgctggg gtcggtttat
     8881 acctggagcc tgtttaatgg cgcgctttct gccaagctgg atgcgccggt aagccaggtc
     8941 gctttctctt tcggcttgtt aagtctgggg ctggcaattt catcttctgt tgcggggaaa
     9001 ttacaggaac gttttggcgt taaacgcgtc actatggctt ccggcatttt gctaggatta
     9061 ggcttcttcc tgacagcgca ttctgacaac ctgatgatgc tgtggttaag cgccggtgtg
     9121 ctggtcggtc tggcggatgg cgcgggttac ctgctgacgc tctctaactg cgtgaagtgg
     9181 ttcccggagc gtaaaggtct gatctccgcg ttcgctatcg gttcttatgg tcttggtagc
     9241 ctgggtttca aatttatcga cacgcagctg ctggaaacgg tgggtctgga aaaaacattt
     9301 gtgatttggg gagcgattgc gctggtgatg attgttttcg gcgcaacgtt aatgaaagac
     9361 gcaccaaaac aggaagtgaa aaccagcaat ggtgtggtgg agaaagatta cacgctggca
     9421 gagtcgatgc gtaaaccgca gtactggatg ttagcggtaa tgttcctgac tgcctgcatg
     9481 agcggcctgt acgtgattgg ggtagcgaaa gatatcgccc aaagtctggc acaccttgat
     9541 gtggtttccg cagccaatgc agtaacggtt atttccatcg ctaacctttc aggtcgtctg
     9601 gtgctgggca ttctgtctga caaaattgcc cgtatccgtg ttatcaccat tggtcaggtg
     9661 atttcgctgg tgggtatggc ggccctgctg tttgcaccat taaatgcagt gacgttcttt
     9721 gcagcgattg cctgcgtggc atttaacttt ggcggcacta ttaccgtctt tccatcactg
     9781 gtcagtgagt tcttcggcct caataacctg gcgaaaaact acggtgtgat ttacctcggt
     9841 ttcggtatcg gtagcatttt tggttcgatt atcgcctcac tgtttggcgg tttctatgtg
     9901 accttctacg tgattttcgc cctgctgatt ctgtcattgg cgctttctac gacgattcgt
     9961 cagccagagc agaaaatgtt gcgtgaggcg catatttaga aatatgttgc tactgaaata
    10021 aatacgagga gggagacttc a
//
LOCUS       DAJTYD010000142         9957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTYD010000142 DAJTYD010000000
VERSION     DAJTYD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.142"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            260..457
                     /locus_tag="OO585_003127"
     CDS             260..457
                     /locus_tag="OO585_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630628.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(407..1600)
                     /locus_tag="OO585_003128"
     CDS             complement(407..1600)
                     /locus_tag="OO585_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6630629.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1736..3460
                     /gene="iucA"
                     /locus_tag="OO585_003129"
     CDS             1736..3460
                     /gene="iucA"
                     /locus_tag="OO585_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6630630.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3461..4408
                     /gene="iucB"
                     /locus_tag="OO585_003130"
     CDS             3461..4408
                     /gene="iucB"
                     /locus_tag="OO585_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6630631.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4408..6150
                     /gene="iucC"
                     /locus_tag="OO585_003131"
     CDS             4408..6150
                     /gene="iucC"
                     /locus_tag="OO585_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6630632.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6147..7484
                     /gene="iucD"
                     /locus_tag="OO585_003132"
     CDS             6147..7484
                     /gene="iucD"
                     /locus_tag="OO585_003132"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6630633.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7490..9685
                     /gene="iutA"
                     /locus_tag="OO585_003133"
     CDS             7490..9685
                     /gene="iutA"
                     /locus_tag="OO585_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6630634.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWIIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2396 a         2565 c         2644 g         2352 t
ORIGIN      
        1 tcataaaaaa catgtcagtg aaaaagaaac ctggcgttgg gttgaaggat ataataaagc
       61 aacagaactg gctcgccaat gccctgatac ccatgtattg tcaatttctg atcgtgaagg
      121 cgatttttat gacctgtttg aacgcgcaga gcagactccc ggaataaaag ctgactggct
      181 tgtcagaatg aaatttaata acagagcaac gctgaatatt aatggtaaac gggatcatag
      241 gttgttgcac gaaagaatca tggagattac accgcagcaa ctggttgaat tcactatacc
      301 tgatgggcgg gggcagcaag ccagaacact gcctgacatg ctttcatcag acgcaggtcc
      361 tctctcatat cctctgattt actccagtaa ggtgatgatc atatctttat ttgatagtac
      421 ggtaatgcca gataaataca gacacaatgg taaataatat agttgcaaat aatgctgcca
      481 taatcagcgc gattgcgaca cctgttttcg ataccagcgg caccagcaaa gcgcacagtc
      541 catagccaag tgtgtgactg gtagcgagcc agcccgcgcc tgcgccatca gccagcttat
      601 cattcagtag aagttgataa gcgggtgttg ccagagctgc tccaaatgac agcactgcac
      661 agcccgggta aaacaaccat agctgttcgg agagcatgat agccagacca ccactcatca
      721 acactccggc actcaggagc agggccaccg gagtcaggcg ctgcggacgg actaccccga
      781 actgcgcgat aagcgcagct actgcggaca gacccaacag ccaggccacc tgttggctaa
      841 tggcggtggt atccgtgacg aactggcgag taagggcagg cgaaagtcca agctgcatca
      901 tgctcaccgc cgcagccagt aatattgcgc aaagcagata aggcagacaa tcccgcttca
      961 gactgacgct cttgcactcc ggtgtgggct gtggtggtgt gccgggcaac cgcagcagca
     1021 tcagcagcgc cagcactggt gctgccatca gtagccccag tggcgccaga ggatgaatgg
     1081 ccagcattgc tgccgcgcac agcggcccga atagcctgcc gcaactcagg ccggagctga
     1141 tggttgccag agcggccatg cgattccctt ctccagctct ctgcaatgcc cagacctgac
     1201 aggctggcac cattgccgac acggtcagcc cgtaggcgat ccgggcgatg attaatattc
     1261 ccagtcccac cgctgttgtt atggcgctgg tagccatcag cacgcttccc agccctagca
     1321 gggtaaagct tatgccatat cctcccagag cccacaacac cacaggttta ctgccagtac
     1381 gggaaatttg ctttccccac cacggggacg atggcagaaa cagcattgag ccaagcatca
     1441 gcagggcagc ccatacagac agactcagat ttgtctgtat gaccagaaca gggagcacaa
     1501 ccagcagacc gttctgtccg ataccgagaa gcccggcact gaacgcaagt ggccagcagg
     1561 acagtgtttt ttggggcgta tcttcgatcc ctggtgacat tataatgctt caactccatg
     1621 tattaattgt gtttatttgt aaaattaatt tatctgacaa taacatttat tgttgataat
     1681 gagaatcatt atcgacataa ttgttattat tttactgtgt gggagctgtt tgactatgac
     1741 cctgccctct gaaaaaccag ccacagatgt ggctgcgcag tgcttcctga atgcactgat
     1801 tcgtgaaacc acagactgga aactgacaga atacccgcca gacgaattgc tcatcccgct
     1861 ggatgagcag aaatcgctcc atttcagagt ggcttatttc tccccaaccc agcatcaccg
     1921 ctttgcattt cctgcacgtc tggtcacggc atcaggcagt tatcctgtcg actttaccac
     1981 tctctcccgg cttattattg ataagctacg ccatcaactg tttctgcccg ttcccctctg
     2041 cgaaactttc caccagcgcg tgctggaaag ccatgtccat acgcaacagg caattgatgc
     2101 ccgtcatgac tgggccgctc tgcgtgaaaa agcgttgaat tttggcgagg ctgagcaggc
     2161 gctgctgaca ggacacgctt tccaccctgc gcctaagtct catgaaccgt ttaaccggcg
     2221 ggaggctgaa cgctacctgc ctgatatggc accccacttc ccactgcggt ggttttcggt
     2281 ggataaaacg caaatcgccg gtgaaagttt gcatctcaac cttcaacagc ggctgacgcg
     2341 atttgccgca gagaatgcgc ctcagttact caacgaatta agtgacaacc aatggctgtt
     2401 cccgttgcac ccgtggcagg gggaatatct tttgcagcag gggtggtgcc aggcacttgt
     2461 tgctaaaggg ctgattaaag acttaggtga ggccggcacg tcgtggctgc cgaccacctc
     2521 ttcccgttcc ctctactgtg ccaccagccg cgatatgatc aagttctccc tgagcgtacg
     2581 gctgaccaac tccatccgta ctctgtccgt gaaagaagtg aagcgtggga tgcgcctggc
     2641 acgcctggct caaaccgacg gctggcagat gctacaggtc cgcttcccga ctttccgggt
     2701 aatgcaggag gatggctggg ccgggctgct cgatcttaac ggcaacatca tgcaggaaag
     2761 tctgtttgcc ctgcgtgaaa atctgctggt ggatcagccg aaaagccaga ctaacgtact
     2821 ggtctccctg actcaggccg cacctgatgg cggtgattcg ctgctggttt cggcggtaaa
     2881 acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc gcccatgcat gggtcgatgc
     2941 atactgtcag caagtgctaa agccgctgtt tacggctgaa gcggattacg gcctggtgct
     3001 gctggcgcat cagcaaaata ttcttgtcca gatgcttggg gatctgccgg tcggatttat
     3061 ttaccgtgac tgtcagggca gcgcttttat gcctcacgcg acagactggc tcgattctat
     3121 tggcgaggcg caggcggaaa atattttcac ccatgagcag ttgctgcgct attttcctta
     3181 ttacctgctg gttaactcca cttttgctgt gaccgctgcg ctgggggctg ccgggctgga
     3241 cagcgaatcg aatctgatgg ctcgtgtacg agcatcgctg gctgaagtgc gtgatcaggt
     3301 gactcataaa acctgtctca actacgtgct ggaaagtccg tactggaacg taaaaggtaa
     3361 ctttttctgt tatctgaacg atcataacga gaacaccatt gttgaccctt cggtgatcta
     3421 tttcgatttc gctaacccgc tgcaggctca ggaggtctga atgtctgagg caaacattat
     3481 tcacagcaga tatggactgc gctgtgaaaa actcgacaag cccctgaatc ttggctgggg
     3541 actggacaat agcgcggtgt tgcactgtcc cggggagctg ccgacagggt ggctgtgcga
     3601 tgcgctggat cagatattta tcgccgcacc acaactgtca gcagtggctc tcccctgggc
     3661 cgaatggcgt gaggagccac aggcgctgac gctttttggg caggtaaaaa gcgacattat
     3721 ccatcgtact gccttctggc agttaccgtt atggttgagt tcaccggcaa accgggcctc
     3781 cggtgaaatg gtttttgatg cagagcgtga gatttatttc ccgcagcgcc caccccgtcc
     3841 acagggtgag gtttaccgcc gttacgatcc acgtattcgc aggatgctga gtttccgcat
     3901 tgccgatccc gtttctgatg cagaacgttt cacccgctgg atgaatgatc cgcgcgttga
     3961 gtatttctgg gagcaaagtg gctcactgga ggtacagacc gcctatctgg agcgccagct
     4021 caccggtaaa catgcattcc cgctgattgg gtgcttcgac gatcggccat tcagctattt
     4081 cgaaatctac tgggcggcgg aagaccgcat tggtcgtcac tattcgtggc agccctttga
     4141 ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc ggggcccact atgtgcaaag
     4201 ctggctgcgc gggctgacac attacctgct gctggatgag ccccgtacgc agcgcacagt
     4261 actggagcca cgcaccgata accagcgcct gttccgccat cttgagcctg cgggataccg
     4321 gacaattaaa gagttcgact tcccacataa gcgctcgcgc atggtgatgg cggatcgtca
     4381 tcacttcttc acggaggtcg gtctgtaatg aatcacaagg actgggattt tgttaaccgt
     4441 cggctggtgg caaaaatgtt gtctgagatg gagtatgagc aggttttcca cgcggaatct
     4501 cagggcgatg accactactg cattaacctg ccgggagcac aatggcgctt catcgctgaa
     4561 cgtggtatct ggggctggct gtggattgat gcccaaacgt tgcgctgcac ggacgaacca
     4621 gtactggcgc agacgctgct gatgcagcta aagccggtgc tgtcaatgag tgatgccact
     4681 gttgcagagc atatgcagga tttgtatgcc acgctactgg gcgacctgca actgctgaag
     4741 gcccgtcgcg gactgagtgc cagtgacctg attaatcttg atgccgatcg tctgcaatgc
     4801 ctgctgagcg gtcatcctaa attcgttttt aataaaggtc gccgtggctg gggcaaagag
     4861 gcgctggaac gatatgcgcc agagtatacc aacaccttca gactgcactg gctggcggta
     4921 aaacgtgaac atatgatctg gcgctgcgat aacgatctgg atattcagca gttgttgact
     4981 gccgcaatgg atccgcagga gtttacacgc ttcagtcagg tctggcagga aaacgggctg
     5041 gatcacaact ggctaccgct gccggtacat ccgtggcagt ggcagcaaaa aatcgccaca
     5101 gacttcatcg ctgattttgt cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag
     5161 tggctcgccc agcagtcgct gcgtaccctg accaacgcca gccgccgggg agggctggat
     5221 atcaagctgc cgctgaccat ctacaacacc tcatgctacc gggggatccc tggcagatat
     5281 atcgctgccg gaccactggc ttcacgctgg ctgcaacagg tttttgcgac cgacgccacc
     5341 ctggtgcaaa gcggcgcagt gatccttggt gaaccggcgg caggctatgt gtcccatgaa
     5401 ggctatgccg cgcttgcccg ggctccctat cgctaccagg aaatgcttgg tgttatctgg
     5461 cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc ctgttctgat ggcaacactg
     5521 atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat atatcgaccg ctccggactg
     5581 gacgctgaaa cctggcttac gcaattgttc cgggtggttg tggttccgct gtatcacctg
     5641 ctttgccgct acggtgtcgc gcttattgca cacggacaaa atataacgct cgccatgaaa
     5701 gagggggtac cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg gctggtgaaa
     5761 gaagcgtttc ccgaaatgga ctctttgcct caggaggtgc gtgatgccac atcccgtctg
     5821 agtgcggact acttaatcca tgatttgcag acgggtcact tcgtgacagt actgcgtttt
     5881 atttcgccac tgatggttcg tcttggcgta cctgaaaggc gattttatca actgctggca
     5941 gcagtgttga gtgattacat gaacaaacat ccacaaatgg cagagcgttt tgcgcttttc
     6001 tcactcttca ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact gacctggccg
     6061 gacctggatg gcggcagtcg catgctgccg aattaccttg agaatctgca aaatccgctg
     6121 tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt cgattttatt ggtgtcggga
     6181 cagggccatt taatctcagc attgctgcat tgtcacatca gatcgaagaa ctgaactgtc
     6241 tcttctttga cgaacatcct catttttcct ggcatccggg tatgctggta ccggattgtc
     6301 atatgcagac cgtctttctg aaagatctgg tcagtgcagt tgcacctaca aatccctaca
     6361 gctttgttaa ctatctggtg aagcacaaaa agttctatcg cttccttaca agcaggctac
     6421 gtacagtatc ccgtgaagag ttttctgact atctccgctg ggctgctgaa gatatgaata
     6481 acctgtattt cagtcatacc gttgaaaata ttgatttcga caaaaaaagt cgattgtttc
     6541 tggttcagac cagtcgggga gaatattttg cccgcaatat ctgccttggt acaggaaaac
     6601 aaccttattt accaccctgt gtgaagcatg tgacacaatc ctgtttccat gccagtgaaa
     6661 tgaatcttcg tcggccggac ctgagtggaa aacggataac cgtggttggt ggaggacaga
     6721 gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg gcggaaataa
     6781 actgggtctc acggcgtaat aattttaacg cactggatga ggctgctttt gctgatgagt
     6841 attttacacc tgaatatatt tcaggcttct ccggactgaa ggaagatatt cgccatcagt
     6901 tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta ctgaccattt
     6961 atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc cgtctgctac
     7021 ccagtcgctc ggtaataact ctggaaagta gtggtccggg ctggaggtta ttgatggaac
     7081 atcatctgga tcagggcaga gagagcctgg aaagtgatgt ggtgattttc gccacaggtt
     7141 accgttctgc attgccacaa atacttccct cactgatgcc cttgatcacc atgcacgata
     7201 agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg aaagaaaata
     7261 acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc cagctcagcc
     7321 tgatggcctg gcgatctgca cgtattctta atcgcgtact ggggcgtgat ttattcgatc
     7381 tcagtatgcc gcccgcactg attcagtggc gcagcggcag ccggaaaaaa ccgcagccgg
     7441 aggctgcttc tttaactcac tatacagcaa atattcagga ataacgatga tgcgcaaaaa
     7501 gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag ctgtcgccca
     7561 gcaaaacgat gataatgaga tcatagtgtc tgccagccgc agcaatcgaa ctgtagcgga
     7621 gatggcgcaa accacttgga ttatcgaaaa tgccgaactg gagcagcaga ttcagggcgg
     7681 taaagagctg aaagacgcac tggctcagtt aatccccggc cttgatgtca gcagccagag
     7741 tcgaaccaac tacggtatga acatgcgtgg ccgcccgctg gttgtcctga ttgacggtgt
     7801 gcgcctcaac tcttcacgtt ccgacagccg acaactggac tctgtcgatc cttttaatat
     7861 cgaccatatt gaagtgatct ccggcgcgac ggccctgtac ggtggcggga gtaccggagg
     7921 gttgatcaac atcgtgacca aaaaaggtca gccggaaacc atgatggagt ttgaggctgg
     7981 cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag cgcattgccg gtgctgtctc
     8041 cggcggaaat gaccatatct ccggacgtct ttccgtggca tatcagaaat ttggcggctg
     8101 gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc tgcagcactc
     8161 caatcggctg gacatcatgg gaaccggtac gctgaacatc gatgaatccc ggcagcttca
     8221 actgataacg cagtactata aaagtcaggg agacgacaat tacgggctta atctcgggaa
     8281 aggcttttcc gccatcagcg ggagcagcac accatacgtc agtaaggggc tgaattctga
     8341 ccgcattccc ggcactgagc ggcatttgat cagcctgcag tactctgaca gtgatttcct
     8401 gggacaggaa ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt actacccgtt
     8461 cccgacggta aatgcgaata aacaggcgac ggctttctcc tcgtcacagc aggataccga
     8521 ccagtacggc atgaaactga ctctgaacag ccaactgatg gacggctggc aaatcacctg
     8581 ggggctggat gctgagcatg agcgctttac ctccaaccag atgttcttcg atctggctca
     8641 ggcaagtgct tccggagggc tgaacaacca taagatttac accaccgggc gctatccgtc
     8701 atatgacatc accaatctgg cggccttcct gcaatccagc tatgacatta atgatatttt
     8761 taccgttagc ggtggcgtac gctatcagta tactgagaac agggtagatg atttcatcga
     8821 ctacacgcag caacagaaga ttgctgccgg gaaggcgata tctgccgacg ccattcctgg
     8881 tggttcggta gattacgata actttctgtt caatgctggt ctgctgatgc acatcaccga
     8941 acgtcagcag gcatggttca atttttccca gggggtggca ttgccggatc cggggaaata
     9001 ttatggtcgc ggcatctatg gtgcagcagt gaacggccat ctttccctga caaagagcgt
     9061 gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac tgggctggcg
     9121 ctttaccggt gacaacctgc ggactcaaat cgcggcatat tactcgcttt ccaataagag
     9181 cgtggaaagg aataaagatc tgaccatcag tgtgaaggac gacaggcgcc gtatttacgg
     9241 cgtggaaggt gcggtggact acctgatccc ggatactgac tggagtaccg gtgtgaactt
     9301 caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg acgtgaagga
     9361 atcaagtcca tcgaaagcga cagcttacat taactgggcg ccggaaccgt ggagtctgcg
     9421 tgtacagagc accacttctt tcgacgtaag cgatgcagag ggtaacgata ttaatggtta
     9481 cactaccgtc gattttatca gtagttggca gcttccggtg ggaacactca gcttcagcgt
     9541 tgagaacctc ttcgaccgtg actataccac tgtctgggga cagcgtgcac ctctgtacta
     9601 cagcccgggt tacggccctg cttcactgta cgactacaaa ggccggggcc gaacctttgg
     9661 tctgaactac tcagtgctgt tctgacgggg atgcttttgc aacaagggta tgctgtaaac
     9721 aacatggtcc ttggtggcca tgttgtttac agccagtata atatacagcc ttaacaagat
     9781 attccgtctc cggaatggac tgttcaccac tgaataacat tatttctgtt ttatggatct
     9841 ttgactgaaa tttatcgcat atcctcattt cattgtccgt ctgaaaacta accttacaag
     9901 taagcgtcgt ctcagcaccg tctggcagat cctgaaattc ctgagaggat gatctgc
//
LOCUS       DAJTYD010000143         9947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTYD010000143 DAJTYD010000000
VERSION     DAJTYD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.143"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1409)
                     /gene="espL1"
                     /locus_tag="OO585_003134"
     CDS             complement(<1..1409)
                     /gene="espL1"
                     /locus_tag="OO585_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6630635.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNA"
     gene            1931..3859
                     /gene="thrS"
                     /locus_tag="OO585_003135"
     CDS             1931..3859
                     /gene="thrS"
                     /locus_tag="OO585_003135"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6630636.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            3863..4405
                     /gene="infC"
                     /locus_tag="OO585_003136"
     CDS             3863..4405
                     /gene="infC"
                     /locus_tag="OO585_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6630637.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4502..4699
                     /gene="rpmI"
                     /locus_tag="OO585_003137"
     CDS             4502..4699
                     /gene="rpmI"
                     /locus_tag="OO585_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6630638.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            4752..5108
                     /gene="rplT"
                     /locus_tag="OO585_003138"
     CDS             4752..5108
                     /gene="rplT"
                     /locus_tag="OO585_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6630639.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5225..5349
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5231..5275
                     /gene="pheM"
                     /locus_tag="OO585_003139"
     CDS             5231..5275
                     /gene="pheM"
                     /locus_tag="OO585_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6630640.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5558..6541
                     /gene="pheS"
                     /locus_tag="OO585_003140"
     CDS             5558..6541
                     /gene="pheS"
                     /locus_tag="OO585_003140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6630641.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6556..8943
                     /gene="pheT"
                     /locus_tag="OO585_003141"
     CDS             6556..8943
                     /gene="pheT"
                     /locus_tag="OO585_003141"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6630642.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8948..9247
                     /gene="ihfA"
                     /locus_tag="OO585_003142"
     CDS             8948..9247
                     /gene="ihfA"
                     /locus_tag="OO585_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6630643.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(9551..9691)
                     /locus_tag="OO585_003143"
     CDS             complement(9551..9691)
                     /locus_tag="OO585_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR6630644.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2597 a         2279 c         2572 g         2499 t
ORIGIN      
        1 aatgcattca gaaagatatc gattgtttca tcgaacccat acaacatgga gatatagagc
       61 ccaggtgttt cgttagcact tttagtttgc agtaatttaa ccagattatc ttcatgaatg
      121 atatgattgt atatcagtga ctgaatttct tgcataaata tttttacgtt gtcagtatgt
      181 ccgttcatca tagccagaaa taaagcaggt gtaccaacgc tattctgcgc agttattatt
      241 tcaatgattt ctttttcagg gagtatgggc aactgtttga ataatgtaat aagctgttga
      301 gtctggcctg tggccattat taacaacatt gccgatggat gtataggggc aaatggcatt
      361 cgtgatgcaa tacccgcgaa aatagcccct ggaactccca caggtaatag gggcagaata
      421 gcaattgcac agttgcgaac atacctatca gcccaggtct gagcatcggg acctgaactg
      481 atgaacttat caagcgaaag aaagccccgt gctgtcctgt gggtgcctct gacggcaacg
      541 ttggtatcgt tcgggtcgta gacggacact acatagttta attgtccata cactaactgc
      601 tttattcgta gccttactga caaagtatga ttgtctatat ataaaatcgc ggacatatga
      661 ctgacattgt ttgccatcat tcgacaaaag aaaatatcta aagcgaagcc aaaatcctta
      721 ctcgcgataa cgcgactccc tgcaggagga tggcgaagta atgctctgaa ctccgcatcc
      781 cggactaagg ggacatgctg ctgtatggat tcaacggagg agtaatgttt aatcaggttg
      841 actttgcctt ttccatttct caatgaatca agggcgtatt gcaatgacag atggcgacaa
      901 aaaatagcgt cagaaggaga ttgcaaaaaa catgcactac cattgagatt tactgttggt
      961 tgtatagttt tttctctatg gattttttta gacagtttca tacaatggtt acgatcttcg
     1021 gcagttaact gttcctgctc aatataatcc agtatgcttc gcaaatggtt agccttaata
     1081 ggcgtggttt ccagaaataa gggaatgatt tcttttttat agtgtaatgt aataagtata
     1141 ttttctgcat ggatgggtga agtggcaaag ttaatgataa aaagggcagt ggagtaacaa
     1201 ttttgtgata tttttcgaca gtttgcggta aattcctgag taatacagta tgacagcatc
     1261 gaacttaagt cagaggtatc aagtagggtg atgagttggc gcgcaaattg attaataagc
     1321 tctgatgaag gcgttggtat gtcgctatcg atgtgcaatc tatgaggaga cttataatga
     1381 gttctgataa tgatatcgtt ttgactcata atctattcac cttatatata ctggaggttt
     1441 tgaggatgat gattaattat tatcgccaga acgaaatgtg cacgaaaaat tgctataaga
     1501 attgtgtcac ttttatcatt gtgatatgac cacttcgtca ttaatctcat gatcgtttaa
     1561 atactaccgc caaatcacgc aacgaacttt gctggattca gtttatgctg ctgtaaatcc
     1621 gctcgcagta aacctttcag acgcaccgtg atgttatcag tggttcttta agcgttttgc
     1681 tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa aggtacaatc
     1741 tccctttttt tagtgttaac atcgttcaac cgggtggaag gtgagaggtt aatgtctttc
     1801 atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt tcgcaatgaa
     1861 ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc actgcaaata
     1921 aggatataaa atgcctgtta taactcttcc tgatggcagc caacgccatt acgaccacgc
     1981 tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag cctgtatcgc
     2041 agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg acgcacaact
     2101 gtcgatcatt accgccaaag acgaagaagg tctggagatc attcgtcact cctgtgcgca
     2161 cctgttaggg catgcgatta aacaactttg gccgcatacc aaaatggcaa tcggcccggt
     2221 tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc aggaagatgt
     2281 cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg tcattaagaa
     2341 gaaagtcagc tggcacgcag cgcgtgaaac tttcgccaac cgtggggaga gctacaaagt
     2401 ctccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact tccatgaaga
     2461 atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc atcatttcaa
     2521 actaatgaaa acggcagggg cttactggcg tggcgacagc aacaacaaaa tgttgcaacg
     2581 tatttacggt acggcgtggg cagacaaaaa agcacttaac gcttacctgc agcgcctgga
     2641 agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt accatatgca
     2701 ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct tccgtgaact
     2761 ggaagtgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta aaggtccgtt
     2821 catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca aagatgcaat
     2881 gttcaccaca tcttctgaga accgtgaata ctgcattaag ccgatgaact gcccgggtca
     2941 cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc gtatggccga
     3001 gtttggtagc tgccaccgta acgagccgtc aggttcgctg catggcctga tgcgcgtgcg
     3061 tggatttacc caagatgacg cgcatatctt ctgtactgaa gaacaaattc gcgatgaagt
     3121 taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg agaagatcgt
     3181 cgtcaaactc tccactcgtc ctgaaaaacg tattggcagc gacgaaatgt gggatcgtgc
     3241 tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat atcaactggg
     3301 tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc tcgatcgtgc
     3361 atggcagtgc ggtacagtac agctggactt ctccttgccg tctcgtctga gcgcctccta
     3421 tgtgggcgaa gacaacgagc gtaaggtacc ggtaatgatt caccgcgcaa ttctggggtc
     3481 gatggaacgt ttcatcggta tcctgaccga agaattcgct ggtttcttcc cgacctggct
     3541 tgcgccagtt caggttgtta tcatgaatat taccgattca cagtctgaat acgttaacga
     3601 attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga gaaatgagaa
     3661 gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc tggtctgtgg
     3721 tgataaagag gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta aagacctggg
     3781 aagcatggac gtaaatgaag tgatcgagaa gttgcaacaa gagattcgca gccgcagtct
     3841 taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg cgcgccctaa
     3901 ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg aaggcgagca
     3961 gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg gagtagactt
     4021 agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt acggcaaatt
     4081 cctctatgaa aagagcaagt cttctaagga acagaagaaa aagcaaagag ttatccaggt
     4141 taaggaaatt aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa aactccgcag
     4201 cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt tccgcggtcg
     4261 tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag acgatttgca
     4321 agaactggca gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga tgatcatggt
     4381 gctcgctcct aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg agccttcggg
     4441 cttcacaggc tttgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg aagttattaa
     4501 aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa ccggtaaagg
     4561 tggttttaag cacaagcacg ctaacctgcg tcacattctg accaaaaaag cgaccaaacg
     4621 taaacgtcac ctgcgtccga aagccatggt ttccaaaggc gatctgggcc tggtaatcgc
     4681 gtgcctgccg tacgcataag ccgttaacgt ttttaacttt ttaattagaa tatagataca
     4741 ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt cacaagaaaa
     4801 ttttgaaaca agctaaaggc tactacggtg cgcgttctcg cgtataccgc gttgccttcc
     4861 aggctgttat caaagctggt cagtatgctt accgtgaccg tcgtcaacgt aagcgtcagt
     4921 tccgtcaact gtggattgca cgtatcaacg cagcagcacg tcagaacggt atttcttaca
     4981 gcaaattcat caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag atcctggctg
     5041 atatcgcagt attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg aaagcagctc
     5101 tggcataagc cagttgaaag agggagcttg tctccctctt ttcgtttcaa cgccatcaaa
     5161 acattgactt ttatcgccgt agccttttca ataaaggtct tttgaagagt accaaaaggt
     5221 aacgcaagca atgaatgctg ctattttccg cttctttttt tactttagca cctgaatcca
     5281 ggaggctagc gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc cagtggaggc
     5341 tttttttgta tgcgcgtttt gaaaaataaa aagccagtct atcaggcgta attgtcgcag
     5401 ccagtctgga tacggccagc gcggagaaac cggagcgtat ttaagtacgt gagaatttcg
     5461 agcactgccc gggaccaaaa tggcaagtaa aatagcctga tgggataggc tctaagtcca
     5521 acgaaccagt gtcaccactg acacaatgag gaaaaccatg tcacatctcg cagaactggt
     5581 tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt gccgcgttag ataatgtgcg
     5641 cgtcgaatat ttgggtaaaa aagggcactt aacccttcag atgacgaccc tgcgtgagct
     5701 gccgccagaa gagcgtccgg cagctggtgc ggttatcaac gaagcgaaag agcaggttca
     5761 gcaggcgctg aatgcgcgta aagcggaact ggaaagcgca gcgctgaatg cgcgtctggc
     5821 ggcggaaacg attgatgtct ccctgccagg tcgtcgcatt gaaaacggcg gtctgcatcc
     5881 ggttacccgt accatcgacc gtatcgaaag tttcttcggt gagcttggct ttaccgtggc
     5941 aaccgggccg gaaatcgaag acgattatca taacttcgat gctctgaaca ttcctggtca
     6001 ccacccggcg cgcgctgacc acgacacttt ctggtttgac actacccgcc tgctgcgtac
     6061 ccagacctct ggcgtacaga tccgcaccat gaaagcccag cagccaccga ttcgtatcat
     6121 cgcgcctggc cgcgtttatc gtaacgacta cgaccagact cacacgccga tgttccatca
     6181 gatggaaggt ctgattgttg ataccaacat cagctttacc aacctgaaag gcactctgca
     6241 cgatttcctg cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc cttcctactt
     6301 cccgttcacc gagccttctg cagaagttga cgtcatgggt aaaaacggta aatggctgga
     6361 agtgctgggt tgcgggatgg tgcatccgaa cgtgctgcgt aacgttggca tcgacccgga
     6421 agtttactct ggtttcgcct tcgggatggg gatggagcgt ctgactatgt tgcgttacgg
     6481 cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac agtttaaata
     6541 aggcaggaat agattatgaa attcagtgaa ctgtggttac gcgaatgggt gaacccggcg
     6601 attgatagcg atgcgctggc gaatcaaatc actatggcgg gcctggaagt tgacggtgta
     6661 gaaccggttg ccggtagctt ccacggcgtg gtcgttggtg aagtggttga gtgtgcgcag
     6721 catccgaacg ctgacaaact gcgtgtgaca aaagtgaatg tcggcggcga tcgcctgctg
     6781 gacatcgtct gtggtgcgcc aaactgccgt cagggcctgc gtgtggcggt agctaccatt
     6841 ggtgctgttc tgccgggtga tttcaaaatt aaagcggcga aactgcgtgg cgaaccgtct
     6901 gaagggatgc tgtgctcctt ctctgaactg ggaatttctg acgatcacag cggcattatc
     6961 gaactgcctg cagatgcgcc gattggcact gacatccgcg aatacctgaa actcgatgac
     7021 aacaccatcg aaatcagcgt aacgccaaac cgtgccgact gcttaggtat cattggtgtt
     7081 gcgcgtgacg ttgccgtgct gaatcagctg ccgctggttg aaccggaaat cgttccggtt
     7141 ggtgcgacca tcgacgacac gttgccgatt acagtcgaag cgcctgaagc ctgcccgcgc
     7201 tatcttggcc gtgtggtaaa aggcattaac gttaaagcgc caactccgct gtggatgaaa
     7261 gaaaaactgc gtcgttgcgg gatccgttct atcgatgcag ttgttgacgt caccaactat
     7321 gtactgctcg aactgggcca gccgatgcac gctttcgata aagatcgcat tgaaggcggc
     7381 attgtggtgc ggatggcaaa agagggcgaa acgctggtgc tgcttgatgg cactgaagcg
     7441 aagctgaatg ctgacactct ggtcatcgcc gaccacaaca aggcgctggc gatgggcggc
     7501 atctttggtg gcgaacactc tggcgtgaat gacgaaacgc aaaacgtctt gctggaatgc
     7561 gctttcttca gcccgctgtc tatcaccggt cgtgctcgtc gtcatggcct gcatactgat
     7621 gcgtctcacc gttatgagcg tggcgttgat ccggcactgc agcacaaagc gatggaacgt
     7681 gcgacccgtc tgctgatcga catctgcggt ggtgaggctg gtccggtaat tgatatcacc
     7741 aacgaagcaa cgctgccgaa gcgtgcaacc atcactttac gtcgtagcaa actggattgc
     7801 ctgatcggcc atcatattgc tgatgaacag gtaactgaca ttctgcgtcg tctcggctgc
     7861 gaagtgaccg aaggcaaaga cgagtggcag gcagttgcgc cgagctggcg tttcgatatg
     7921 gagattgaag aagatctggt cgaagaagtc gcgcgtgttt acggctacaa caacatcccg
     7981 gatgagccgg tacaggcaag cctgattatg ggtactcacc gtgaagctga cctgtcgctc
     8041 aagcgcgtga aaacgctgct caacgacaaa ggctatcagg aagtgatcac ctacagcttc
     8101 gttgatccga aagtgcagca gatgatccat ccaggcgttg aagccttact gctgccaagc
     8161 ccgatctctg ttgaaatgtc agcaatgcgt ctttctctgt ggaccggcct gctggcaacc
     8221 gtggtgtaca accagaaccg tcagcagaac cgtgtgcgca ttttcgaaag cggtctgcgt
     8281 ttcgtaccag atactcaggc accgttgggc attcgtcagg atctgatgtt agccggtgtg
     8341 atttgcggta accgttacga agagcactgg aacctggcaa aagagaccgt tgatttctat
     8401 gatttgaaag gcgatcttga atccgttctc gacctgaccg gtaaactgaa tgaggttgag
     8461 ttccgtgcag aagcgaatcc ggcactgcat ccggggcaat ccgcagcgat ttatctgaaa
     8521 ggggaacgta ttggttttgt tggagttgtt catcctgaac tggaacgtaa actggatctt
     8581 aacggtcgca ctctggtgtt cgaactggag tggaacaagc tcgcagaccg cgtggtgcct
     8641 caggcgcgcg agatttctcg cttcccggcg aaccgtcgtg acatcgcggt ggtggtcgca
     8701 gaaaacgttc ccgcagcgga tattttatcc gaatgtaaga aagttggcgt aaatcaggta
     8761 gttggcgtaa acttatttga cgtgtaccgc ggtaagggtg ttgcggaggg gtataagagc
     8821 ctcgccataa gcctgatcct gcaagatacc agccgtacac tcgaagaaga ggagattgcc
     8881 gctaccgtcg ccaaatgtgt agaggcatta aaagagcgat tccaggcatc attgagggat
     8941 tgaacctatg gcgcttacaa aagctgaaat gtcagaatat ctgtttgata agcttgggct
     9001 tagcaagcgg gatgccaaag aactggttga actgtttttc gaagagatcc gtcgcgctct
     9061 ggaaaacggc gaacaggtga aactctctgg ttttggtaac ttcgatctgc gtgataagaa
     9121 tcaacgcccg ggacgtaacc cgaaaacggg cgaggatatt cccattacag cacggcgcgt
     9181 ggtgaccttc agacccgggc agaagttaaa aagccgggtc gaaaacgctt cgcccaaaga
     9241 cgagtaatca gatctaacta aaaaggccgc tctgcggcct tttttctttc aataactaat
     9301 tgatttataa tgttttatat ctcttcgttc cacgatgcgt ccacatttag tggggggagg
     9361 ggagtaagcc cgctacattg tgggctttgt cttatagtct gatcactgtt tcaagtcggt
     9421 gaggacacca aaaagaaccc ccgcagtttt tacgctgcgg gtttgttgtt catgtctgtg
     9481 agatagggtg ccttatcgac cttaccctgg caaccgattg acgggggatt gctcccccgt
     9541 cgcggtttcc ttactgctta cactgtaaga acgccgcaaa ctccgctccc cagaagctca
     9601 tccgtatttc gcacagcgaa ccgtgcagca tccagatgat gaggattgcc gtcacgcaga
     9661 acgtgatggc cgtaagcgat ttttgcgaca tagcgcttgc tcctttttcg gggaggcgct
     9721 aacctatcac ttgcttaggg tagacggtta gggcctcggt taaacaaaaa tgttttccgg
     9781 ggcctttcca catctggcct tcaggtattc cctccgacca tcagccgaaa ggcacccgcg
     9841 cgtaatctat cgcttttttg ttactccggc aattctgctc gttaattctg aggtaagggc
     9901 aaactcatct gattgtttcc tctgtgtgaa gttggcagtt catgcca
//
LOCUS       DAJTYD010000144         9943 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTYD010000144 DAJTYD010000000
VERSION     DAJTYD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.144"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OO585_003144"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OO585_003144"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6630645.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQACIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OO585_003145"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OO585_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6630646.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OO585_003146"
     CDS             1309..1830
                     /locus_tag="OO585_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6630647.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMEMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO585_003147"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO585_003147"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6630648.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OO585_003148"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OO585_003148"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6630649.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OO585_003149"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OO585_003149"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6630650.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OO585_003150"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OO585_003150"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6630651.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OO585_003151"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OO585_003151"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6630652.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="OO585_003152"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="OO585_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="OO585_003153"
     CDS             complement(7360..8175)
                     /locus_tag="OO585_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6630653.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="OO585_003154"
     CDS             complement(8186..9445)
                     /locus_tag="OO585_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6630654.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..9943)
                     /locus_tag="OO585_003155"
                     /pseudo
     CDS             complement(9455..9943)
                     /locus_tag="OO585_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="multicopy suppressor of dominant negative ftsH
                     mutations; putative acyl-CoA synthetase; internal stop;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2184 a         2819 c         2664 g         2276 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgtgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atggaaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgatgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgattttac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgtggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgg
//
LOCUS       DAJTYD010000145         9727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTYD010000145 DAJTYD010000000
VERSION     DAJTYD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.145"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            7..1620
                     /gene="trg"
                     /locus_tag="OO585_003156"
                     /pseudo
     CDS             7..1620
                     /gene="trg"
                     /locus_tag="OO585_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(1658..2580)
                     /locus_tag="OO585_003157"
                     /pseudo
     CDS             complement(1658..2580)
                     /locus_tag="OO585_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2797..4140
                     /locus_tag="OO585_003158"
     CDS             2797..4140
                     /locus_tag="OO585_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6630655.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGPLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4365..6020
                     /gene="opgD"
                     /locus_tag="OO585_003159"
                     /gene_synonym="mdoD"
     CDS             4365..6020
                     /gene="opgD"
                     /locus_tag="OO585_003159"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6630656.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6160..6384
                     /locus_tag="OO585_003160"
     CDS             6160..6384
                     /locus_tag="OO585_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6630657.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            6447..6983
                     /gene="rimL"
                     /locus_tag="OO585_003161"
     CDS             6447..6983
                     /gene="rimL"
                     /locus_tag="OO585_003161"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6630658.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(6978..7958)
                     /locus_tag="OO585_003162"
                     /pseudo
     CDS             complement(6978..7958)
                     /locus_tag="OO585_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8082..9074
                     /gene="tehA"
                     /locus_tag="OO585_003163"
     CDS             8082..9074
                     /gene="tehA"
                     /locus_tag="OO585_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6630659.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9071..9664
                     /gene="tehB"
                     /locus_tag="OO585_003164"
     CDS             9071..9664
                     /gene="tehB"
                     /locus_tag="OO585_003164"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6630660.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         2476 a         2291 c         2494 g         2466 t
ORIGIN      
        1 acggtaatga atacaactcc ctcacagcga ttaggttttt tgcatcacat caggctggtt
       61 ccgttatttg cctgcattct tggcggtatc ttagttctat tcgcattaag ttctgctctg
      121 gcaggccgat cgcgatcagc gtgatgttac tgcggagatt gagattcgga ccgggttagc
      181 gaacagttca gattttttgc gttcagcccg gatcaatatg attcaggccg gggctgcgag
      241 tcgtattgcg gaaatggaag caatgaagcg aaatattgcg caagccgaat cggagattaa
      301 acagtcgcag caaggttatc gtgcttatca gaatcgaccg gtgaaaacac ctgctgatga
      361 agccctcgac actgaattaa atcaacgctt tcaggcttat atcacgggta tgcaacctat
      421 gttgaaatat gccaaaaatg gcatgtttga agcgattatc aatcatgaaa gtgagcagat
      481 ccgaccgctg gataatgctt ataccgatat tttgaacaaa gccgttaaga tacgtagcac
      541 cagagccaac caactggcgg aactggccca tcagcgcacc cgcctgggtg ggatgttcat
      601 gattggcgcg tttgtgcgtg ccctggtcat gacgctgata acatttatgg tgctacgtcg
      661 gatcgtcatt cgtccactgc aacatgccgc acaacggatt gaaaaaatcg ccagtggcga
      721 tctgacgatg aatgatgaat cggcgggtcg taatgaaatc ggtcgcttaa gtcgtcattt
      781 acagcaaatg cagcattcac tggggatgac agtagggact gttcgacagg gcgcggaaga
      841 gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg gacctgtcat ctcgcaccga
      901 agaacaagcg gcggctatcg aacaaactgc cgccagcatg gagcaactca ctgcgacggt
      961 gaaacagaat gcggataacg cgcatcatgc cagcaaactg gcgcaagagg cttctattaa
     1021 agccagcgat ggcgggcaga cggagtaaaa acgatgggcg ctatctccac gagttcgaag
     1081 aaaatttctg agatcaccgc cgtcatcaac agtattgctt tccagacgaa tattctggca
     1141 ctgaatgctg ccgttgaagc cgcgcgagcg ggtgagcaag ggcgtggatt tgccgttgtc
     1201 gccagcgaag tacggacact cgcaagtcgc agcgctcagg cggcgaaaga gattgaaggc
     1261 ttgatcagtg aatcagtcag gttaattgac ctggggtcgg atgaggtggc aacggccggg
     1321 aaaaccatga gcactattgt tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc
     1381 gccgccgcct cggatgaaca aagtagaggc ataacgcagg ttagccaggc gatttctgaa
     1441 atggataagg tgacgcaaca gaatgcttct ctggtagaag aggcctcagc ggcggcggtg
     1501 tcccttgaag aacaggcggc acgattaact gaggcggtgg atgtattccg tctgaacaaa
     1561 cattctgtgt cggcagaacc tcgcggagcg ggtgaaccag ttagtttcgc tacggtgtga
     1621 aaatgttcaa ggagggatcg acagatccct tcacctttca gaacggcatt gattttcgaa
     1681 tagcgttaat catcaactgg caaccagaag agaacatcgc atctacgcgg gtcagtattc
     1741 caatcggttc gcctgcacca tgtcccggaa caggctgggc caccagcgtg gcatgacgca
     1801 ggtcgtcttt taccgcgcca gaagggacaa accacacgta gtcgtattca accgtaagtt
     1861 gacgagatag cgaagcagac agcgtttcga tacaacccga aggaatttta catccctggc
     1921 tctgtactaa tgcatctgaa tgctggcgtg gcgcagtgcc ttctggtgat acaacgaccg
     1981 gccatttcag cactcggctt agcgttacgt tctcctgaag tagcgggtga ttagggcgga
     2041 caaccagctt cagcgattca agaaacagca gttcgtaatt aagcccggtc atcagttcag
     2101 gatctgacat ccgaccaatg ccgatatcga tttccccggt tttcaaaccc gccagaatca
     2161 tagggttact cattgtcgca acttgcaagg tcgtctcttt ttgttgctga tgaaactgac
     2221 ctataaccga aggtaatatc cccagtgccg cagtaggtag tgcaccaatc ctgacgacat
     2281 cattattaag accttcttta cgatgaagcg actgtccggc agtgttgatg gcgtcaagaa
     2341 ctctgactgc atgcgttaaa aattgttcgc cgggtaaggt aagttgcgcc ccctgacgac
     2401 cacgctcaaa caagcgagcg cccgtcagct gctacagttc attcaatgtc ttagagagcg
     2461 caggttgact caaattaagg gtttcagccg cgcccccaaa gttccttgtt gtgcgacagc
     2521 tacgaatgta tgaaggtggc gcaaacggat gcgctgacta aacagactat ttttttccat
     2581 aagcgatgtt aaaaacgaag cggtgtcgct gacaagtgaa gttgtttgat tatgataact
     2641 tgattgcaaa atataattaa caattaaagc aattgtgtta cagcaaaatg gataatattg
     2701 atgttttcgc ggcgagatca cagtttgtaa attcttcccg ccagagtgaa tgcggttacc
     2761 tacactccag attactgacc actggaggca gacactatgg cgaacagcat cacggcggat
     2821 gagattcggg aacagttttc gcaggcaatg tcagccatgt accagcaaga agttccgcag
     2881 tacggcccgc tgctggaact ggtagctgat gtgaatctgg ctgtgctgga aaacaatcct
     2941 caactgcacg aaaaaatggt aaatgcagat gagctggcgc gactgaatgt tgaacgtcat
     3001 ggggcgattc gcgttgggac tgcacaagag cttgctactc ttcggcggat gtttgccatt
     3061 atggggatgt acccggtgag ctattacgat ctctcgcagg caggggggcc ggtacattcg
     3121 acagcatttc ggcccattga tgatgcttct ctggcgcgta atcccttccg cgtttttacc
     3181 tccttactcc gccttgagct tatcgagaac gaaattttgc gccagaaagc ggcggagatt
     3241 ctgcgtcagc gcgatatctt caccccacgt tgtcgacaac tgttagagga atatgagcag
     3301 cagggcggtt ttaacgaaac acaggcacag gaatttgtgc aagaggccct ggaaacgttt
     3361 cgctggcacc agtcagcaac ggtagatgaa gaaacctatc gcgccttgca caacgaatat
     3421 cggttgattg ctgatgtggt ctgttttcct ggatgccata tcaaccacct gacgccacgt
     3481 acgctggata ttgaccgggt gcagtcgatg atgcctgaat gcggaattga acccaaaatt
     3541 ctcatcgagg ggccgccgcg ccgcgaggta ccgattttac tacgccagac cagctttaaa
     3601 gcactggaag agacggtgtt gtttgcgggg cagaaacagg gcacgcatac cgcgcgcttt
     3661 ggtgaaattg agcagcgtgg cgtggcatta acgccgaaag ggcgacaact gtatgatgat
     3721 cttctgcgta acgctggaac cgggcaggat aatctcactc accaaatgca tttacaggaa
     3781 accttccgca cttttcctga cagtgagttt ttaatgcgtc agcaagggtt ggcatggttc
     3841 cggtaccgtc tgacgccttc gggtgaggcg catcgtcagg cgattcatct tggagacgat
     3901 ccacagcctt taattgaacg tggttgggta gtggcgcaac ccatcaccta tgaagatttc
     3961 ttgcccgtta gcgcggcggg gatcttccag tcaaatctgg gtaatgaaac gcagacacgc
     4021 agtcacggta atgccagtcg cgaagcattt gagcaggcgt tgggttgtcc ggttttggat
     4081 gagttccagc tttaccagga agcggaagaa cgcagtaaac gtcgctgtgg tttgctttaa
     4141 attctgacca tccgcctttg caaaaatttg cctgatttta caaacgaatc aggctcatcc
     4201 catcgacata aaaaaaatgc cgatttatgc atattctctc agttcaacaa ttggattatt
     4261 aataaatatt gtctagagtg agcggtcata aataagcact ttcttgccgc tgaaaacgac
     4321 cagcgcggga ccattcacaa caccagaagg actcactttc aggtatggat cgtagacgat
     4381 ttattaaagg ttcaatggct atggccgccg tgtgcggtac cagcggcatt gcttctcttt
     4441 tttctcaggc ggcattcgcg gcagattctg atattgccga cgggcaaacc cagcgttttg
     4501 acttctccat tctacagtca atggcgcacg acttagcgca aacagcgtgg cgtggtgcgc
     4561 ctcgtccgtt acctgacacg ctggcgacaa tgacgccgca ggcttataac agtattcaat
     4621 acgacgccga aaaatcgctc tggcataacg ttgagaaccg tcaactggac gctcagttct
     4681 tccatatggg aatgggattc cgtcgccgcg ttcgtatgtt ttctgtagat ccagcaacac
     4741 atctggcgcg tgaaattcac tttcgcccgg agttgttcaa atacaacgat gcgggtgttg
     4801 atacaaaaca attagaaggg caaagcgatc tcggctttgc cggttttcgc gtgtttaaag
     4861 cccccgaact ggcgcgccgt gatgtagtat catttctcgg cgcgagttat ttccgcgccg
     4921 ttgatgatac atatcaatac ggtttgtcgg cccgcggcct ggcgatcgac acttacaccg
     4981 acagtaaaga agagttcccc gactttaccg ccttctggtt tgatacggta aaaccggggg
     5041 caactacctt taccgtttat gcgttgctcg atagcgccag cattactggt gcctataagt
     5101 tcactatcca ttgtgagaaa agtcaggtga ttatggatgt ggaaaatcac ctgtatgcgc
     5161 gcaaagacat taaacagctg ggcattgcgc cgatgaccag tatgttcagc tgcggtacta
     5221 atgaacgtcg gatgtgcgat acaattcatc cgcaaattca tgactctgat cgtctgtcca
     5281 tgtggcgggg caacggcgag tggatttgcc gtccgctgaa taatccgcaa aaattgcagt
     5341 tcaatgctta caccgacaac aacccgaaag ggtttggttt attgcaactg gatcgtgact
     5401 tctcccatta tcaggacatt atgggctggt ataacaaacg cccaagtctg tgggtggaac
     5461 cgcgtaacaa gtggggtaag ggcaccatcg gcctgatgga aatcccaaca acgggcgaaa
     5521 cgctggataa cattgtctgt ttctggcagc cagaaaaagc tgtaaaagca ggtgatgagt
     5581 ttgcattcca gtatcgtctg tactggagtg cgcaaccgcc tgttcattgc ccattagcgc
     5641 gcgttatggc gacgcgtacc ggcatgggcg gtttcccgga aggttgggcg ccaggtgaac
     5701 actatcccga aaaatgggcg cgtcgttttg ccgtcgattt cgttggtggt gatctgaaag
     5761 ctgccgcgcc aaaaggcatt gagccggtga ttacgctttc cagtggggaa gcgaagcaaa
     5821 tcgaaattct ctatattgaa cccatcgatg gttatcgtat tcagtttgac tggtatccga
     5881 cttcggactc cactgatccg gtcgatatgc ggatgtatct acgttgtcag ggggacgcta
     5941 tcagtgaaac atggctgtat cagtatttcc cgccagcgcc ggataaacgt cagtatgttg
     6001 acgaccgcgt gatgagttaa ttgttttttc ttcggcacct tcttcgggag gtgccgtctg
     6061 gttaaacacg atcccgctcg catttttccc taagttaaat gagtaatctg atggtgtgta
     6121 tttcagatac accttgtcag ccactaacag ggagtgcgta tgtttccaga ataccgagat
     6181 ttaatatccc gtctgaaaaa cgaaaatcct cgctttatgt ccttgttcga taaacacaat
     6241 aaacttgatc atgaaattgc cagaaaggaa ggtttcgacg gtcgagggta caatgcggaa
     6301 gtggtccgca tgaaaaaaca aaagctacag ttaaaagatg agatgctcaa aatcctgcag
     6361 caggagagcg tcaaaggggt gtaaactttc ctaagccgcc agccaggcgg cttttttaac
     6421 aacagcatgg attgactgga gataagatga ctgaaacgat aaaagtaagc gaatcacttg
     6481 aattacatgc tgttgcggaa agtcacgtca cacctcttta tcagttaatc tgtaaaaata
     6541 aaacctggtt acagcagtcg ctaaactggc cgcagttcgt tcagagtgaa gaggacacgc
     6601 gaaaaacggt gcagggtaat gtgatgttgc atcaacgcgg ctatgccaaa atgttcatga
     6661 ttttcaaaga agatgaactt atcggcgtta tctcgtttaa tcgtattgaa ccactgaata
     6721 aaactgctga aatcggctac tggctggacg aatctcatca ggggcagggg atcatttctc
     6781 aggcgctgca ggcattgatt catcattacg ctcagtctgg tgaacttaga cgcttcgtga
     6841 tcaaatgtcg ggtggacaat ccgcaaagca accaggtcgc tttgcgcaat ggttttatcc
     6901 ttgaaggttg cctgaaacag gctgagttcc tgaatgatgc ctatgatgat gtgaacttat
     6961 acgcgcgtat tatcgattca taataaccct gccagcggcg ttcgcgtaat gcgctcctcg
     7021 ccgttaatga cttttggacc acggacatga accgtgtcgc catcaaaggc ttcaacgacg
     7081 gcgtggtcgg ttaactccac atggttttca attatcaccg cacctgaaat acggctctca
     7141 ccttgtatta caacgtgctc gtcgagcaga atcggttcac cacgtaccac ggcatttccg
     7201 ccaatcaaga catgatgttt taacacacaa ttaccttcca caatggcata ttccgccacc
     7261 tgcgaactgt aatgaatcgt cggaatggca tcttcttcta tgccagcttt cacctgcgcg
     7321 tgaccgtaga ctttagcgca atcgcatagc cagacgttgt tctcttcatt accttcaaca
     7381 ctggcaaaat caaaaacttc ggcgcgatgt tcaataaaag cataccggac gacggcatcg
     7441 ccataaattt gtgcctggtg gacaatacgc gaggcgctaa cccttgcgcg atcataaatt
     7501 tggagcagga gttgatggtc gggcgttaag ccttgtgcgg cgacgatcat agaatgttga
     7561 tcaattaagg cgtgaccaaa tattctacat tgcccgtaaa ctaatgagtc atgaatggtg
     7621 acactgtcac ttatatatgc accctgactg atttcactat tatcgatcca gacgttatcc
     7681 gttgcgtaaa cctctcccca cagcacgctg gtgccggtga tacgcgtatt accagaaatc
     7741 accgttccgc cgaacgcaat agcattctgg tcgtaaatcc agcaatttcc ttcctatgct
     7801 aacaccgttt cgcgatcgat ccagccgcca gcggttcctg ctatcacatc gttaaaatcg
     7861 ctaatggcga taatctgccg taataacaca ctttttttag tgccatcttc ctgataacta
     7921 aaggctcgct gttcttcact tagacgatat ttgcgcatag cgttttctca caggtgactt
     7981 actataaccg tagcaaattc tgcggctctg gctatgctcg agaaaattcc ataaaatgca
     8041 tttcaaatat actttataaa ttaaacaaaa tgagtaagaa gatgcagagc gataaagtgc
     8101 tcaatttgcc ggcaggctac tttggtattg tgttggggac gatagggatg ggatttgcct
     8161 ggcgctatgc cagccaggtt tggcaggtca gccactggtt aggggatggg ctggtgattc
     8221 tggcgatgat catctgggga ttattaacta gcgcatttat tacccgactc atacgctttc
     8281 cgcatagcgt gctggcggaa gttcgccatc cagtgctgag cagttttgtg agtttgtttc
     8341 cggcaacgac gatgctggtg gcgattggtt ttgttccgtg gtttcgccca ctggcggtgt
     8401 gcctgttcag ttttggtgtc gtggttcagt tggcttatgc cgcctggcaa actgcgggat
     8461 tatggcgcgg atctcaccct gaagaagcta ccatgcctgg actgtatctg ccgacagttg
     8521 ccaacaactt tatcagcgca atggcctgtg gtgcgttggg ctacaccgac gccggtctgg
     8581 tgtttttagg cgcaggcgtt ttctcatggc taagccttga accggtgatc ttgcagcgtc
     8641 tgcgcagttc gggagaatta cccacggcac tgcggacatc actcggcatt cagctcgctc
     8701 ctgcgctggt ggcctgtagt gcctggttga gcgtcaacgg cggcgagggt gatacgctgg
     8761 cgaaaatgct ttttggttat ggactgctgc aactgctgtt tatgctacgt ctgatgccat
     8821 ggtatctctc ccagccattt aatgcttcat tctggagttt ctcgttcggc gtatctgcac
     8881 tggcaaccac cggtttgcat ctggggagtg gcagcgaaaa tggatttttc catacgctgg
     8941 cggtaccgct gtttatcttt accaatttta ttattgcaat actgctcatc cgtacttttg
     9001 cgcttctgat gcagggaaaa ttgttagtca gaaccgagcg cgctgtttta atgaaagcag
     9061 aggacaaaga atgatcattc gtgacgaaaa ctattttact gataaatatg aattaacccg
     9121 cacacactct gaagtactgg aagcggtgaa agtggttaaa ccgggtaaaa cgctggatct
     9181 gggctgtggc aatggtcgta acagtcttta cctggcagcc aatggttatg atgttgacgc
     9241 atgggataaa aatgccatga gtatcgccaa tgtagagcgc attaaatcca ttgaaaatct
     9301 ggataattta cacacccgag tcgttgatct gaataacctc acatttgata gacagtacga
     9361 ttttattctt tcgactgtgg tactgatgtt ccttgaggct aaaaccatcc cagggctgat
     9421 tgccaatatg caacgttgca ctaaacttgg tggttacaac ctgattgtgg cggcgatgga
     9481 taccgctgat tatccatgta ccgtcggctt cccgtttgcc ttcaaagagg gagaattacg
     9541 tcgatattac gaaggctggg agagggtgaa atacaatgaa gacgtcggcg agctgcaccg
     9601 caccgacgcc aacggtaatc gtattaaact gcgtttcgcc acgatgctgg cacgtaaaaa
     9661 atgacccgct aagaataagg cgcgtgaaaa ttccccacgc tgagatgatt tactgttctt
     9721 cttttcg
//
LOCUS       DAJTYD010000146         9647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTYD010000146 DAJTYD010000000
VERSION     DAJTYD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.146"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..600
                     /locus_tag="OO585_003165"
     CDS             <1..600
                     /locus_tag="OO585_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6630661.1"
                     /translation="RHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQMIALRIM
                     QQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSWIVQP
                     VANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSRIICRDKQLIFEEAP
                     QAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            597..1211
                     /locus_tag="OO585_003166"
     CDS             597..1211
                     /locus_tag="OO585_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6630662.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1268..2725)
                     /gene="pepD"
                     /locus_tag="OO585_003167"
     CDS             complement(1268..2725)
                     /gene="pepD"
                     /locus_tag="OO585_003167"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6630663.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2986..3444
                     /gene="gpt"
                     /locus_tag="OO585_003168"
     CDS             2986..3444
                     /gene="gpt"
                     /locus_tag="OO585_003168"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6630664.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3536..4780
                     /gene="frsA"
                     /locus_tag="OO585_003169"
     CDS             3536..4780
                     /gene="frsA"
                     /locus_tag="OO585_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6630665.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4838..5239
                     /gene="crl"
                     /locus_tag="OO585_003170"
     CDS             4838..5239
                     /gene="crl"
                     /locus_tag="OO585_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6630666.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5278..6333)
                     /gene="phoE"
                     /locus_tag="OO585_003171"
     CDS             complement(5278..6333)
                     /gene="phoE"
                     /locus_tag="OO585_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6630667.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6621..7724
                     /gene="proB"
                     /locus_tag="OO585_003172"
     CDS             6621..7724
                     /gene="proB"
                     /locus_tag="OO585_003172"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6630668.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7736..8989
                     /gene="proA"
                     /locus_tag="OO585_003173"
     CDS             7736..8989
                     /gene="proA"
                     /locus_tag="OO585_003173"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6630669.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9104..9179
                     /locus_tag="OO585_003174"
     tRNA            9104..9179
                     /locus_tag="OO585_003174"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9137..9139,aa:Thr,seq:cgt)
     gene            complement(9194..>9647)
                     /locus_tag="OO585_003175"
     CDS             complement(9194..>9647)
                     /locus_tag="OO585_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6630670.1"
                     /translation="PEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPD
                     GTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGG
                     WESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2330 a         2382 c         2537 g         2398 t
ORIGIN      
        1 cggcatattg cctcgttctc gcatcatggt ttgcctcaag gcagtgacga cgcgctacgg
       61 tatattgcgg aacatgacga tgcgctggcg tttgcgtgta ttaatcgcca gatgatcgct
      121 ttgcgcataa tgcaacaagt taaggccacg ggaagtccgg ttctggatgt ggcgcataac
      181 tttgttagcg cgtgtcaaat cggcgatcaa cagggctggt tgcatcgtaa aggtgccacg
      241 ccggatgaca acggtctggt gattattccc ggttcacgtg gtgattactc ctggattgta
      301 cagcctgttg cgaacgaaaa aacgctccac tcgctggcgc atggggcagg gcgtaaatgg
      361 gggcgcaccg agtgtaaatg gcgtctggca gcgaaataca cggtgacgca actctcgcgg
      421 actgaacttg gcagtcggat aatttgtcgc gataaacaac tcatctttga agaagcgcca
      481 caagcctata aatcggctga aagcgtggtg caatgtctgg tgcaggccgg tttaattatt
      541 cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa aaaaggatga
      601 tcttgctaca actctcctca gcgcaggggc cggaagaatg ttgtctcgca gtgaaaaagg
      661 cgctggacag gctaattaaa gaagctgccc gacaagacgt cgcggtaacg gtgttggaaa
      721 cagaaacggg ccgctactct gacacgctgc gttcagctct ggtttctctg gatggcgata
      781 acgcatgggc attaagcgaa agctggtgtg gcactattca gtggatttgt ccgagtccgt
      841 atcggcctca tcatgggcgc aaaaactggt ttctgggcat tgggcgtttt accgctgatg
      901 agcaggagca atccgatgcg atccggtatg agacgctgcg ttcgtcgggg ccgggcggtc
      961 aacatgtcaa taaaaccgac tcggcggtac gcgccacgca tttggcatcc ggtattagcg
     1021 tgaaggttca gtcagagcgt agtcagcatg ctaacaagcg gctggcacga ttgctgattg
     1081 cctggaagct ggagcagcag caacaggaaa atagcgcggt gctgaaatcg cagcggcgaa
     1141 tgttccatca ccagattgaa cgtggcaacc cgagacggac atttacaggg atggctttta
     1201 tcgaaggata atgaaggatg aaactgccgg aaggcgatta aacgccatcc ggcagcaaat
     1261 caaataatta cttcgccgga atttctttca gcagttcagt cagcagtgtc cagtaatgac
     1321 ctacgctttc gatgtgaact tgctcatccg gagagtgtgg accggtgata gttggcccga
     1381 tagaaaccat gtccatttcc ggatacggtt ttttgaacag accacattcc aggcccgcgt
     1441 ggataatctg gatgttcggc gtcttgttga acaggcgctg ataggtttca cgtaccagat
     1501 gcatcaccgg agaattagcg tccggctgcc agccaggata tgcgcctttc gcttcggttt
     1561 tcgcgccagc cagtttaccc agcgaatcca gcatgctcac cacgtagtct ttaccgctgt
     1621 cgatcagtga acggatcagg cagtgaattt ctacgttatt gtcagtcatg gtcaccacac
     1681 cgacgttcag ggaggtttca accacacctt tggctacatc ggagttgcgg atcacaccgt
     1741 tcggggtggc gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc agggcagctt
     1801 tatcgttcgc tacagagtcc agcaacaagg ccagattttt ctctttctct gccagctcgt
     1861 ttttcaggat ctcctgatag gtattcacca gagatttcag tgcgtcgact ttatcagctg
     1921 cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg ccgccgttga
     1981 aatcgataag gcgcaggtcc agttcttctg catgacccgc caggaagcgc accagcagtt
     2041 tgttggcatt acccaggcca acgtggattt caccgccgga gtgaccgcct ttcagacctt
     2101 ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct aaatgcaggt
     2161 tggaggtgaa gtcgataccc cccgcacaac ccatgtagat ttcaccttct tcttcggagt
     2221 cggtgttaat cagaatatca gcctgcaacc agttgctctg taagccaaaa gcaccgtcca
     2281 taccggcttc ttcggtcatg gtcagcagca cttccagcgg accgtgaacc acgttttcgt
     2341 cagccagaac cgccagtgca gaggccatac caatgccgtt atccgcaccc agcgtggtgc
     2401 cgcgcgcttt aacccattcg ccatcaatat aaggctggat aggatctttc gtgaagtcat
     2461 gcacggtgtc gttatttttc tgcggcacca tatcgaggtg ggcctgtaag acgaccggtt
     2521 tacgattttc catacctgcg gtagcaggtt tacgaatcag gatattacct acctgatcgc
     2581 gttcgacatg gaaacctttc tcttttgccc aaccaacaat gtattcagcg agttgctctt
     2641 catgatagga cgggtgcgga atagaacaga ttttggcaaa aatatcccac agcggctgtg
     2701 gagataattg agacagttca gacacgttaa gtctccttgt cgatcacccg caaaacagta
     2761 ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa gacaggcttg cgagatatgt
     2821 ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca
     2881 tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc
     2941 tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac
     3001 atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct
     3061 tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc gggtgcgtta
     3121 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     3181 gacaaccagc gagagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcata
     3241 gttattgatg acctggtgga taccggtggt actgctgttg cgattcgtga aatgtatcca
     3301 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     3361 gttgttgata ttccgcaaga tacctggatc gaacagccgt gggatatggg cgtcgtattc
     3421 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     3481 tttaacttca ggcaggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     3541 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     3601 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc ttgatggtaa
     3661 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggtattga
     3721 cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg aacgtaccga
     3781 cgatgattta tacgatactg tgattggcta ccgtggtggc aactggattt atgagtgggc
     3841 cacccaggcg atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc aactcagtgg
     3901 tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc atctgaaagg
     3961 agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag aggccgccca
     4021 gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg cgcccatcac
     4081 cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa tgtgtggtgg
     4141 tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg cgccgcgcgg
     4201 cattgtgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat ggaagctcac
     4261 ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg taccgtgggt
     4321 ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg ccgtgcgtct
     4381 ggcatatctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg tagttcatac
     4441 cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg acgttctggc
     4501 gagtcgtttg gggatgcatg atgcttccga cgaagcgtta cgcgtggagc tgaatcgcta
     4561 ttcattaaaa gtgcaaggat tgctgggacg tcgctgccca acgccaatgt tatcaggcta
     4621 ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct catcatctgc
     4681 tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg acaaaggtct
     4741 tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa aaatttgcta aattttgcca
     4801 atttggtaaa acagttgcat cacaacagga gatagcaatg acgttaccga gtggacaccc
     4861 gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg aaggtaagtg
     4921 cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga aaccggcacc
     4981 ggaagtgcgt gaattctggg gctggtggat ggagctggaa gcgcaggaat cccgttttac
     5041 ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagtg ttccggtaaa
     5101 agatactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg agaagctgcg
     5161 tgaactgctg acgacgctga atctgaagct ggaaccggcg gatgattttc gtgacgagcc
     5221 ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca atatctatta
     5281 aaactgatac gtcatcccaa tcgcgacaat atcatcatta ttaatattca atttgttatc
     5341 gctatccagt tggttgattt tataatcaac aaatgctgac atatttttgt tgaaataata
     5401 cgtagcaccg acgtcgatat aattaaccag atcttcacca ccgatgccct caatatcttt
     5461 ccctttcgat aagacataac ccagcgatgg acgcagacca aagtcaaact ggtattgagc
     5521 gaccgcttca aagttctgtg tcttattggc aaagccgcca gttattggcg tcattttgcg
     5581 agtttcagaa tagaaagttg ccagataaat attattggca tcgtatttca gacctgttgc
     5641 ccatgcttct gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt tggtgcgatc
     5701 tgagttggta taggccccac taatggcgaa atcgctgccg ccaaagtcat atgtcaatga
     5761 cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt tcccttgata
     5821 ttgcagggtt aagttcaggc catcgataac gccgaagaag tcggtgttcc gatacgtcgc
     5881 cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc gccgaggagt cgccaccaaa
     5941 ttccggaaac atatcggtcc aggcttccac gtcatacaac gcgcccaggt tacgaccata
     6001 atcgaaagaa cccaaatctt tatatttcaa cccggcaaaa gcgagacgcg ttttttgctg
     6061 tgcagtatca ctctctgctt tattaccggc aaactctgct tcccaacgac cataaccagt
     6121 cagttgatcg ttaatttgtg tttcgccttt aaaaccaaaa cggatataac tctggtcgcc
     6181 atctttactg tcgttatcac tcatataatg catggcttta actttgccat agacatccag
     6241 tttattaccg tctttgttat atatttctgc ggcctgtacg gatgcagatg ccacaatgcc
     6301 catcaccact aatgccagag tgctcttttt cattttcatt cctgatttta attaacgcgc
     6361 gaatattcag cgggagagcc ccgttgaaaa caggaaagtt tttaacctga gattgttaaa
     6421 gatatattac agattaataa tattcttaaa atgtggtaat ttattaaatc tgtaataaaa
     6481 gcgtaaacaa ctgccgctag gcttgctgat cccgcgcaac aaaacgccat gctttgctcg
     6541 cagatggttg gcaaccgacg acagtcctgc taaaacgttc gtttgatatc atttttccta
     6601 aaattgaatg gcagagaatc atgagtgaca gccagacgct ggtggtaaaa ctcggcacca
     6661 gtgtgctaac aggcggatcg cgccgtctga accgtgccca tatcgttgaa cttgttcgcc
     6721 agtgcgcgca gttacatgcc gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg
     6781 ccgccggacg tgagcacttg ggttacccgg aactgccagc gactatcgcc tcgaaacaac
     6841 tgctggcagc ggtagggcag agtcgactga ttcaactgtg ggaacagctg ttttcgattt
     6901 atggcattca cgtcgggcaa atgctgctga cccgtgctga tatggaagac cgtgaacgct
     6961 tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca
     7021 atgagaacga tgctgtcgct acggcagaga ttaaagtcgg cgataacgat aacctttctg
     7081 cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat caaaaaggtt
     7141 tgtataccgc tgacccgcgc agcaatccgc aggcagaact gattaaagat gtttacggca
     7201 ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga actggcggca
     7261 tgagtaccaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac accattattg
     7321 ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc gtcggtacgc
     7381 tgttccatgc ccaggcgact ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc
     7441 cggcgggtga aatcacggta gatgaagggg caactgccgc cattctggaa cgcggcagct
     7501 ccctgttgcc gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc
     7561 gcatttgcaa cctcgaaggt cgcgatatcg cccacggtgt cagtcgttac aacagcgatg
     7621 cattacgccg tattgccgga caccactcgc aagaaattga tgcaatactg ggatatgaat
     7681 acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagct ggccgatgct
     7741 ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg
     7801 cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat
     7861 tctcaacgct aacgcccagg atgttgctga cgcgcgagcc aatggcctta gcgaagcgat
     7921 gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgacg atgtacgtca
     7981 ggtgtgcaac ctcgccgatc cggtggggca ggtaatcgat ggcggcgtac tggacagcgg
     8041 cctgcgtctt gagcgtcgtc gcgtaccgct gggagttatt ggcgtgattt atgaagcgcg
     8101 cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg cggtgatcct
     8161 gcgcggtggc aaagaaacgt gtcgcactaa cgctgcaaca gtggcggtga ttcaggacgc
     8221 cctgaaatcc tgcggcttac cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc
     8281 gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc cgcgtggtgg
     8341 cgctggtttg cataaactgt gccgtgaaca gtcgacaatc ccggtgatca caggtggtat
     8401 aggcgtatgc catatttacg ttgatgaaag tgtagagatc gctgaagcat taaaagtgat
     8461 cgtcaacgcg aaaactcagc gtccgagcac atgtaatacg gttgaaacgt tgctggtgaa
     8521 taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt
     8581 gacattacac gcagatgcag ctgcactggc gcagttgcag gcaggccctg cgaaggtggt
     8641 tgctgttaaa gccgaagagt atgacgatga gtttctgtca ttagatttga acgtcaaaat
     8701 cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac aacactccga
     8761 tgcgatcctg acctgcgata tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc
     8821 cgctgtttac gttaacgcct ctacgcgttt taccgacggc ggccagtttg gtctgggtgc
     8881 ggaagtggcg gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc tggaagcact
     8941 gaccacttac aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg
     9001 tgatgcaaaa gtagccattt gattcacaag gccattgacg catcgcccgg ttagttttaa
     9061 ccttgtccac cgtgattcac gttcgtgaac atgtcctttc agggccgata tagctcagtt
     9121 ggtagagcag cgcattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccat
     9181 ttaaatcaat aagttacaca tcattagtac gttccttatt tcttgactgg gacaaatttg
     9241 ggaccgatgg gttcagaatc gagtctattt gccgtgcgtg ttcggtaagg tgattaggtg
     9301 cgaggtgagc atatcgacga accatttcga tagactccca gcctcccatt tcctgtaaca
     9361 ctgataacgg gactccggct tgaaccagcc aacttgccca ggtgtgtctc aagtcgtgaa
     9421 atctgaaatc atcaatacct gctcgtctca gcgccgcttt ccaggctgtg tttgcgtcat
     9481 accgcatctt cctgactgtt ggcgctttcg ttccgtctgg tttggtacag ctttccttat
     9541 acacaaatac ccaacggtga tgattcccga tttgtttttt caatacgcga catgcagtat
     9601 cattcagcgc aacgccaatt gcgcggtttg atttactctc ttccggg
//
LOCUS       DAJTYD010000147         9565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTYD010000147 DAJTYD010000000
VERSION     DAJTYD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.147"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..696)
                     /locus_tag="OO585_003176"
     CDS             complement(<1..696)
                     /locus_tag="OO585_003176"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6630671.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGN"
     gene            868..1521
                     /gene="rluE"
                     /locus_tag="OO585_003177"
     CDS             868..1521
                     /gene="rluE"
                     /locus_tag="OO585_003177"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6630672.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            1531..1992
                     /gene="nudJ"
                     /locus_tag="OO585_003178"
     CDS             1531..1992
                     /gene="nudJ"
                     /locus_tag="OO585_003178"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6630673.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2046..3152
                     /gene="mnmA"
                     /locus_tag="OO585_003179"
     CDS             2046..3152
                     /gene="mnmA"
                     /locus_tag="OO585_003179"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6630674.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3188..3829
                     /gene="hflD"
                     /locus_tag="OO585_003180"
     CDS             3188..3829
                     /gene="hflD"
                     /locus_tag="OO585_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6630675.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            3833..5203
                     /gene="purB"
                     /locus_tag="OO585_003181"
     CDS             3833..5203
                     /gene="purB"
                     /locus_tag="OO585_003181"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6630676.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5371..6042
                     /gene="phoP"
                     /locus_tag="OO585_003182"
     CDS             5371..6042
                     /gene="phoP"
                     /locus_tag="OO585_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6630677.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6042..7502
                     /gene="phoQ"
                     /locus_tag="OO585_003183"
     CDS             6042..7502
                     /gene="phoQ"
                     /locus_tag="OO585_003183"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6630678.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            7578..8699
                     /gene="roxA"
                     /locus_tag="OO585_003184"
                     /gene_synonym="ycfD"
     CDS             7578..8699
                     /gene="roxA"
                     /locus_tag="OO585_003184"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6630679.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(8748..>9565)
                     /gene="pepT"
                     /locus_tag="OO585_003185"
     CDS             complement(8748..>9565)
                     /gene="pepT"
                     /locus_tag="OO585_003185"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6630680.1"
                     /translation="GADDKAGIAEIMTALAVLQQKNIPHGDIRVAFTPDEEVGKGAKH
                     FDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIVGNNVHPGTAKGVMVNALSLAA
                     RIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMHYIIRDFDRKQFEARKCKMMEI
                     AKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILDIAQQAMRDCDIEPELKPIRGG
                     TDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEKAVQVIVRIAELTAQRK"
BASE COUNT         2319 a         2457 c         2456 g         2333 t
ORIGIN      
        1 gttgcctttg tgcaccagag tcacagagtc acgatcgtta gcaattgcgt attcgatcgc
       61 tgcacgaacc agacgtttgg tgccttcttc cgaacacggc ttaataccga taccacaatg
      121 ttccgggaag cgaattttct tcacccccat ctcttcacgc aggaatttaa tcactttctc
      181 ggcgtcggca gagtctgctt tccattcgat acccgcataa atgtcttccg agttttcacg
      241 gaagataacc atatcggtca gttcagggtg tttaaccggg cttggagtgc cctgatagta
      301 acgtaccgga cgcaggcaga tgtagagatc cagttcctgg cgcagggcaa cgttcagaga
      361 gcgaataccg ccaccaacag gagtggtcag cggaccttta atggcaacgc gatattcacg
      421 aatcagatca agggtttcag caggcagcca gacatcctga ccataaacct gtgtggattt
      481 ttcaccggtg taaatttcca tccaggagat tttacgctcg cctttatagg ctttctcgac
      541 tgcagcgtcg accactttca gcatggctgg ggttacatct acaccgattc catcaccttc
      601 aatgtaaggg ataatcggat tttcaggaac gttgagtttg ccgttttgca gggtgatctt
      661 cttgccttgt gccggaacaa ctactttact ttccattcac ctctccttcg agcgctactg
      721 gtttgctcgt ctgccaccac gttgcatatg cgtttgcgtc ctgcgatacg gatgctttag
      781 agcaattttt tgttaatgat ttgtaattgg cttgtcaata ctaccctatt gtttgccgtc
      841 atgaaagatg cgcgttatta ggctataatg cggcaattca taatctctga aaataccatg
      901 caaaaaactt cttttagaaa tcacctggtt aagcgattca gctcgcaacg ttctaccagg
      961 cgtaaacctg aaaaccagcc cacgcgtgtg atcctgttca ataaacccta cgatgttctt
     1021 ccgcagttca ccgatgaagc cggacgcaaa acattaaaag aattcatccc ggttcagggc
     1081 gtttatgcag caggtcgtct tgaccgcgat agcgaagggt tactggtgct gaccaataac
     1141 ggcgcgttgc aggcgcgttt aacccagccg ggcaaacgca ccgggaaaat ctattatgtg
     1201 caggtggaag gtattcccac acaagacgca cttgaagcct tgcgcaatgg cgtaacctta
     1261 aatgatggcc ctaccctgcc cgccggcgcg gagctggttg acgaaccagc gtggttatgg
     1321 ccgcggaatc caccaattcg tgaacgcaaa agtattccca ccagctggct gaagatcacc
     1381 ttatatgaag gacgtaatcg ccaggtgcgc cgcatgaccg cccatgttgg tttccccacg
     1441 ctgcgactga ttcgttatgc gatgggtgat tacactttgg ataaccttgc caatggcgaa
     1501 tggcgagaag caacagaata aggatgcaca atgtttaaac cacacgttac cgttgcttgc
     1561 gtagtgcacg cagagggtaa atttttagtc gttgaagaga cgattaatgg taaagcgtta
     1621 tggaaccaac ctgccgggca tctggaagcc gatgaaacct tagtggaagc tgccgcccgt
     1681 gagctatggg aagaaaccgg catcagcgcg caaccgcaac actttattcg tatgcatcag
     1741 tggattgcgc cagataaaac gccgtttttg cgtttcctct ttgccattga gcttgagcaa
     1801 atatgcccga cccagcctca tgacagcgat atcgactgct gtcgttgggt cagcgccgaa
     1861 gaaattttaa aggcgtcaaa tcttcgttcg ccgctggtgg cggaaagtat ccgttgttat
     1921 caaagcgggc aacgctatcc gctggagatg attggcgatt ttaactggcc ttttacaaag
     1981 ggtgtcatct aaaaggctgc gtgatagaat acgccgcctt gaagttcaat gtcgtgagtg
     2041 atccaatgtc tgaaaccgca aaaaaagtaa tcgtcggcat gtccggcggt gtcgattcct
     2101 ccgtttctgc ctggctgttg caacaacagg gatatcaggt tgaaggcctg tttatgaaga
     2161 actgggaaga agacgacggt gaggaatatt gcacagcggc agcggatctg gctgatgccc
     2221 aagctgtctg cgacaagctc ggcattgaac tgcacaccgt taactttgct gccgagtact
     2281 gggacaacgt cttcgaactg ttccttgccg aatataaagc cggtcgcacg ccgaatccgg
     2341 atattctgtg caacaaagag atcaaattta aagctttcct cgaatttgcc gccgaagatt
     2401 taggtgccga ttatatcgct accggtcatt acgtacgtcg ggccgatgtc gatggcaaga
     2461 gccgcctgct gcgtggtctg gacagcaata aagaccagag ctacttcctt tatacgctca
     2521 gccatgagca gattgcgcaa agcctgttcc cggtcggcga actggaaaaa ccgcaggtgc
     2581 gtaagattgc tgaagatctt ggtctggtca ccgcgaagaa aaaagactct accggcattt
     2641 gcttcattgg cgaacgtaaa ttccgcgagt tcctgggccg ttatctcccg gcgcaaccgg
     2701 gcaaaatcat taccgtcgat ggcgatgaaa ttggtgagca ccaggggctg atgtatcaca
     2761 ctctcggtca gcgtaaaggt ctgggtatcg gtggcaccaa agaaggcacc gaagaaccgt
     2821 ggtatgtggt ggacaaagac gtcgaaaaca acattctggt tgttgcgcag ggccatgaac
     2881 acccgcggct gatgtctgtc gggttgattg cccagcagtt gcactgggtc gatcgcgaac
     2941 cattcaccgg tactatgcgt tgcacggtaa aaacccgcta tcgccagacc gatatccctt
     3001 gcaccgtcaa ggcgctggac gatgatcgca ttgaagtgat tttcgatgaa ccggttgccg
     3061 ccgtgacgcc gggccagtct gccgtcttct ataacggtga agtgtgcctc ggtggcggta
     3121 ttattgagca gcgtctgccg ctgccggtct gattattatc tttacttaaa acaaggaagc
     3181 agtgaacgtg gcaaagaatt actatgacat caccctcgcc ctggccggta tttgtcagtc
     3241 ggcacgcctg gtgcaacaac tcgctcacca ggggcattgt gatgccgatg cgctacacgt
     3301 ctcactcaac agtattattg atatgaaccc cagctcgacg ctggcggttt ttggcggtag
     3361 cgaagccaac ctgcgcgtcg ggctggaaac cctgctcggc gtgctcaatg ccagcagtcg
     3421 ccagggctta aacgccgaat taacccgcta cacactcagc ttgatggtgc ttgagcgcaa
     3481 actctcctca gcgaaaggcg cgctcgacac tctgggcaac cggatcaacg gcctgcaacg
     3541 ccagctcgaa cacttcgatt tacagtccga aacgctgatg agcgcgatgg ctgctatcta
     3601 tgttgatgtg atcagcccgc ttggcccgcg cattcaggtc accggttccc ctgctgtact
     3661 gcaaagccca caagtgccgg cgaaagttcg cgcaaccctg ctggcaggca ttcgcgccgc
     3721 cgtgctctgg caccaggtcg gcggcggacg tctgcaactg atgttttctc gtaatcgcct
     3781 gaccactcag gcaaaacaaa ttcttgctca tttaaccccg gagttgtgat ctatggaatt
     3841 atcctcactg accgccgttt cccctgtcga tggacgctac ggcgataaag tcagcgcgct
     3901 gcgcgggatt ttcagcgaat atggtttgct gaaattccgt gtacaagttg aagtacgttg
     3961 gctgcaaaaa ctggccgcgc acgcagcgat caaggaagtt cctgcttttg ctgccgacgc
     4021 aatcggttac cttgatgcaa ttgtcgccaa tttcagcgaa gaagatgccg cacgcatcaa
     4081 aaccatcgag cgtaccacta accacgacgt taaagcggtt gagtatttcc tgaaagaaaa
     4141 agtggcggag atcccggaac tgcacgcggt ttctgaattc atccactttg cctgtacttc
     4201 ggaagatatc aataacctct cccacgcatt aatgctgaaa accgcgcgtg atgaagtgat
     4261 cctgccgtac tggcgtcaac tgattgatgg cattaaagat ctcgccgttc agtaccgcga
     4321 tatcccgctg ctgtctcgta cccacggtca gccagccacg ccgtcaacca tcggtaaaga
     4381 gatggcaaac gtcgcctacc gtatggagcg ccagtaccgc cagcttaacc aggtggagat
     4441 cctcggcaaa atcaacggcg cggtcggtaa ctataacgcc cacatcgccg cttacccgga
     4501 agttgactgg catcagttca gcgaagagtt cgtcacctcg ctgggtattc agtggaaccc
     4561 gtacactacc cagattgaac cgcacgacta cattgccgaa ctgtttgatt gcgttgcgcg
     4621 cttcaacacc attctgatcg actttgaccg tgacgtctgg ggttatatcg cccttaacca
     4681 cttcaaacag aaaaccattg ctggtgagat tggttcttcc accatgccgc ataaagttaa
     4741 cccgatcgac ttcgagaact ccgaagggaa tctgggcctt tccaacgcgg tattacagca
     4801 tctggcaagc aaactgccgg tttcccgctg gcagcgtgac ctgaccgact ccaccgtgct
     4861 gcgtaacctc ggcgtgggta tcggttatgc gctgattgcg tatcaatcca ccctgaaagg
     4921 cgtgagcaaa ctggaagtga accgtgacca tctgctggat gagctggatc acaactggga
     4981 agtgctggct gagccaatcc agacagttat gcgtcgctat ggcatcgaaa aaccgtacga
     5041 gaagctgaaa gagctgactc gtggtaagcg cgttgacgcc gaaggcatga agcagtttat
     5101 cgacggtctg gcgttgccag aagaagagaa agcccgcctg aaagcgatga cgccggcaaa
     5161 ctacattggt cgcgccatca ccatggttga tgagctgaaa taaactcgta tcagtgccgg
     5221 atggcgatgc tgtccggcct gcttattaag attatccgct ttttattttt tcactttacc
     5281 tcccctcccc gctggtttat ttaatgttta cccccataac cacataatcg cgttacacta
     5341 ttttaataat taagacaggg agaaataaaa atgcgcgtac tggttgttga agacaatgcg
     5401 ttgttacgtc accaccttaa agttcagatt caggatgctg gtcatcaggt cgatgatgca
     5461 gaagatgcca aagaagccga ttattatctc aatgaacatt taccggatat tgcgattgtc
     5521 gatctcggat tgccagacga ggacggtctg tcactgattc gccgctggcg tagcaatgat
     5581 gtttcactgc cgattctggt attaaccgcc cgtgaaagct ggcaggacaa agtcgaagta
     5641 ttaagtgctg gtgctgatga ttacgtgact aaaccatttc atattgaaga ggtgatggcg
     5701 cgaatgcagg cattaatgcg gcgtaatagc ggtctggctt cacaggtcat ttcgctacct
     5761 ccgtttcagg ttgatctctc tcgccgtgaa ttatctatta atgacgaagt gatcaaactg
     5821 accgcgttcg aatacaccat tatggaaacg ttgatacgca ataatggcaa agtggtgagc
     5881 aaagattcgt taatgctcca actctatccg gatgcggagc tgcgggaaag ccataccatt
     5941 gatgtactga tgggacgtct gcgcaaaaaa attcaggcac aatatccgca agaagtgatt
     6001 actaccgttc gcggccaggg ctatttgttc gaattgcgct gatgaaaaaa ttactgcgtc
     6061 tttttttccc gctctcgctg cgggtacgtt ttctgttggc aacggctgcg gtagtactgg
     6121 tgctttcgct tgcctacgga atggtcgcgc tgatcggtta tagcgtcagt ttcgataaaa
     6181 ctacgtttcg gctgttacgt ggcgagagca atctgttcta tacccttgcg aagtgggaaa
     6241 acaataagtt gcatgtcgag ttacccgaaa atatcgacaa gcaaagcccc accatgacgc
     6301 taatttatga tgagaacggg cagcttttat gggcgcaacg tgacgtgccc tggctgatga
     6361 agatgatcca gcctgactgg ctgaaatcga atggttttca tgaaattgaa gcggatgtta
     6421 acgataccag cctcttgctg agtggagatc attcgataca gcaacagttg caggaagtgc
     6481 gggaagatga tgacgacgcg gagatgaccc actcggtggc agtaaacgtc tacccggcaa
     6541 catcgcggat gccaaaatta accattgtgg tggtggatac cattccggtg gagctaaaaa
     6601 gttcctatat ggtctggagc tggtttatct atgtgctctc agccaatctg ctgttagtga
     6661 tcccgctgct gtgggtcgcc gcctggtgga gtttacgccc catcgaagcc ctggcaaaag
     6721 aagtccgcga actggaagaa cataaccgcg aattgctcaa tccagccaca acgcgagaac
     6781 tgaccagtct ggtacgaaac ctgaaccgat tgttaaaaag tgaacgcgaa cgttacgaca
     6841 aataccgtac gacgctcacc gacctgaccc atagtctgaa aacgccactg gcggtgctgc
     6901 aaagtacgct gcgttctctg cgtagtgaaa agatgagcgt cagtgatgct gagccggtaa
     6961 tgctggagca aatcagccgc atttcacagc aaattggcta ctacctgcat cgtgccagta
     7021 tgcgcggcgg gacattgctc agccgcgagc tgcatccggt cgccccactg ctggacaatc
     7081 tcacctcagc gctgaacaaa gtgtatcaac gcaaaggggt caatatctct ctcgatattt
     7141 cgccagagat cagctttgtc ggtgagcaga acgattttgt cgaggtgatg ggcaacgtgc
     7201 tggataatgc ctgtaaatat tgcctcgagt ttgtcgaaat ttctgcaagg caaaccgacg
     7261 agcatctcta tattgtggtc gaggatgatg gccccggtat tccattaagc aagcgagagg
     7321 tcattttcga ccgtggtcaa cgggttgata ctttacgccc tgggcaaggt gtagggctgg
     7381 cggtagcccg cgaaatcacc gagcaatatg agggtaaaat cgtcgccgga gagagcatgc
     7441 tgggcggtgc gcggatggag gtgatttttg gtcgccagca ttctgcgccg aaagatgaat
     7501 aaatatgtcc atacttcacg cattacgtta agcatccgtt ataatcggtt gcagatacca
     7561 gcctgtggat gcttaacatg gaataccaac tcactcttaa ctggcccgat tttcttgaac
     7621 gtcactggca gaaacgcccg gtggtgttaa aacgcggctt taataatttt attgacccga
     7681 tctctccaga cgagttggcg ggtctggcga tggaaagcga agttgacagt cgactggtca
     7741 gtcaccagga tggcaaatgg caggtcagcc acggcccgtt cgaaagctac gatcatctcg
     7801 gtgaaaccaa ctggtcatta ctggtacagg cagtgaacca ctggcatgag ccgaccgccg
     7861 cgctgatgcg accgttccgt gaactaccgg actggcgtat tgatgatctg atgatttctt
     7921 tttctgtacc tggcggcggc gtcggcccgc atctcgatca gtacgacgtg tttatcattc
     7981 agggtaccgg acgtcgtcgc tggcgagtgg gcgaaaagct gcaaatgaaa cagcactgcc
     8041 cacatccgga tctgttacag gtcgatccgt tcgaagccat catcgatgaa gagctggagc
     8101 ctggtgatat tctttatatt ccgccaggat tcccgcatga aggctacgcg ctggaaaatg
     8161 cgatgaacta ttccgtgggc tttcgcgcgc caaatacgcg ggaactgatt agtggatttg
     8221 ccgattatgt gctgcaacgt gaactgggcg gcaactacta cagcgatcct gatgttccac
     8281 ctcgcgctca tcctgcggat gttctgcctc aagagatgga taaactgcgt gagatgatgc
     8341 tcgaattgat caaccagccg gaacacttta agcaatggtt tggcgagttt atatcccagt
     8401 cacgtcatga actggatatc gcgccgccgg aaccgcctta tcagccagat gaaatctacg
     8461 atgcgctgaa acaaggtgat gtgctggtgc gcctgggtgg tctgcgcgta ttgcgcattg
     8521 gcgacgacgt gtatgccaat ggtgagaaga tcgattcccc gcaccgtccg gcactggatg
     8581 cactcgccag caacattgcg ctgactgcgg agaattttgg cgatgcgctg gaagatccgt
     8641 cattcctcgc gatgctcgcg gcgctggtca atagcgggta ttggttcttc gaagggtaag
     8701 tttggattta agccggatgc ggcatcacat gccgcatccc ttttcgctta cttccgttgc
     8761 gccgttaact cggcaatacg gacgatcacc tgcaccgctt tttccatacc ttccagagtc
     8821 acaaactcat gcttaccatg atagttgtaa ccgccagtga acaagttcgg gcacggtaat
     8881 cccataaacg acaactgcgc gccgtcggta ccgccgcgga tcggtttcag ttccggttca
     8941 atatcgcagt cacgcattgc ctgctgggcg atatcgagaa tatgcggatg ctcaaccact
     9001 ttctcgcgca tattgtagta actgtcttca atcaccagtt caatgtagca atcaggatgt
     9061 aaccctttgc ccactttttt ggcgatctcc atcattttac atttacgcgc ttcaaactgt
     9121 ttacggtcga aatcacggat gatgtagtgc atatcggccc gttcaacggt gcctttcatg
     9181 cccgccaggt gatagaaacc ttcatagcct tctgtcattt ccgggctttc atccgccgga
     9241 acttccgcat gaatacgtgc cgccagcgac agcgcattta ccatcactcc tttcgccgtg
     9301 cccggatgaa cattgttacc gacaattttg atattgaccg atgcggcgtt gaagttttca
     9361 aactccagtt cgcctacgcc accaccgtca acagtgtaag cccagcgggc atcgaaggca
     9421 tcaacatcaa aatgtttcgc ccctttgccc acttcttcat ccggggtaaa ggcgacgcga
     9481 atatcaccat gcggaatgtt tttctgttgc aataccgcca gcgcggtcat gatttctgca
     9541 atacctgctt tgtcatcggc accta
//
LOCUS       DAJTYD010000148         9546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTYD010000148 DAJTYD010000000
VERSION     DAJTYD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.148"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1153
                     /gene="kdpA"
                     /locus_tag="OO585_003186"
     CDS             <1..1153
                     /gene="kdpA"
                     /locus_tag="OO585_003186"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCR6630681.1"
                     /translation="LWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALSADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1176..3224
                     /gene="kdpB"
                     /locus_tag="OO585_003187"
     CDS             1176..3224
                     /gene="kdpB"
                     /locus_tag="OO585_003187"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6630682.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3233..3776
                     /gene="kdpC"
                     /locus_tag="OO585_003188"
                     /pseudo
     CDS             3233..3776
                     /gene="kdpC"
                     /locus_tag="OO585_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            3769..6453
                     /gene="kdpD"
                     /locus_tag="OO585_003189"
     CDS             3769..6453
                     /gene="kdpD"
                     /locus_tag="OO585_003189"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6630683.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            6450..7127
                     /gene="kdpE"
                     /locus_tag="OO585_003190"
     CDS             6450..7127
                     /gene="kdpE"
                     /locus_tag="OO585_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6630684.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(7343..7600)
                     /locus_tag="OO585_003191"
     CDS             complement(7343..7600)
                     /locus_tag="OO585_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630685.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            7723..>9546
                     /gene="speC"
                     /locus_tag="OO585_003192"
     CDS             7723..>9546
                     /gene="speC"
                     /locus_tag="OO585_003192"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6630686.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNV"
BASE COUNT         2117 a         2463 c         2671 g         2295 t
ORIGIN      
        1 gttatgggtg ctagtccctg tggcgttgtt gatcgcactg ttttttattc aacaaggtgc
       61 gctgcaaaac tttctgccct atcaggcagt gaataccgtt gaaggagcgc aacagctgtt
      121 acccatggga ccagtagctt ctcaggaagc gatcaagatg ctcggtacta acggcggtgg
      181 cttctttaat gccaactcgt cgcatccgtt tgaaaaccca accgcactga ccaacttcgt
      241 gcagatgctg gcgatcttct tgatcccaac agctctgtgc tttgcctttg gtgaagtgac
      301 gggcgatcgc cgccaggggc gcatgttgct gtgggcgatg tcagtgattt ttgtcatctg
      361 cgtaggcgtg gttatgtggg cagaagttca gggtaatcct catctgctgg cgctcagcgc
      421 agacagcagc atcaatatgg aaggtaaaga gagccgtttc ggcgtgctgg tcagtagcct
      481 gtttgcggtc gtgactacgg cggcgtcctg tggcgcggtg attgcgatgc atgattcgtt
      541 taccgctctc ggcggcatgg tgccgatgtg gctaatgcaa attggtgaag tggtgttcgg
      601 cggtgtcggt tctggtcttt acggcatgat gctgtttgtc ctgctggcgg tgtttattgc
      661 cgggctgatg attggtcgta caccggaata tctgggtaaa aaaatcgacg tacgcgagat
      721 gaaactgact gcactggcga ttctggttac tccaacactg gtactgatgg gcgcggcgtt
      781 ggcgatgatg accgacgccg gacgtagcgc catgcttaac cctggcccgc atggttttag
      841 cgaagtgctg tacgccgtgt cgtccgccgc taacaacaac ggcagcgcct ttgccggatt
      901 aagcgccaac tctccgttct ggaactgttt actggcgttc tgcatgtttg tcggtcgctt
      961 cggggtgatt atcccggtga tggcaattgc cggttcgctg gtgagtaaaa agagccaacc
     1021 cgccagctcc ggcacgctgc caacgcacgg cccgctgttt gttggcctgt taatcggcac
     1081 cgtgttgctg gttggcgcac tgacctttat ccctgccctg gcgcttggtc cggtggcgga
     1141 atatctctcc tgatgatatt gagtgagcac tgaatatgag tcgtaaacaa ctggcgctat
     1201 tcgaaccaac acttgtcgtt caggcgctga aagaagcggt gaaaaaatta aacccgcagg
     1261 cgcaatggcg caatccggtg atgtttatcg tctggatcgg cagtctgctg accacctgta
     1321 ttagcatcgc gatggcaagc ggtgcgatgc ctggcaatgc gctgtttagc gcggcaatta
     1381 gcggctggct gtgggtcacc gtactgttcg ctaatttcgc cgaggcgctg gcagaaggcc
     1441 gcagtaaagc gcaggccaac agtcttaaag gggtgaaaaa aactgccttt gcccgcaagc
     1501 tgcgtgagcc gaaatatggc gctgcggcgg acaaagttcc tgccgatcaa ctgcgtaaag
     1561 gtgacatcgt gctggtagaa gctggcgata ttatcccctg cgatggtgaa gtcattgaag
     1621 gcggcgcatc ggtcgatgaa agcgccatca ccggggaatc ggcaccggtg atccgtgaat
     1681 ccggcggcga ttttgcctcc gtcaccggcg gcacgcgtat tctttctgac tggctggtga
     1741 ttgagtgtag cgttaacccc ggtgagacat ttctggatcg gatgatcgcg atggtggaag
     1801 gcgcacagcg acgcaaaaca ccgaacgaga ttgccctgac tatcctgctg attgctctga
     1861 ccatcgtctt tttactggca accgccacgc tgtggccgtt ttccgcgtgg ggcggtaatg
     1921 ccgtgagtgt aacggtactg gtggcgctgc tggtctgtct gatcccaacc actattggcg
     1981 gcctgttgtc agcgatcggc gtcgccggga tgagtcggat gctgggcgcg aatgtgattg
     2041 ccaccagcgg acgtgcagtt gaagcggcag gtgacgttga cgttctgcta ctggataaaa
     2101 ccggcaccat cacactcggt aaccgtcagg cgtcggagtt tatccctgcg cagggcgtgg
     2161 atgaaaaaac gctggctgac gccgcacaac tggcttcgct ggctgatgaa acgccggaag
     2221 gccgcagtat tgtgatcctc gccaagcaac gttttaacct gcgcgagcgc gatgtgcagt
     2281 cgctccatgc cacctttgta ccgtttactg cgcaaagccg gatgagcggg atcaacatcg
     2341 acaaccgcat gatccgtaaa ggttctgtcg atgccattcg ccgccatgtt gaggctaacg
     2401 gcggtcactt ccctgccgat gttgatcaaa aagtcgatca ggttgcgcgt cagggggcca
     2461 cgccgctggt ggtggtggaa ggttcccgtg tgctgggcgt tattgcgctg aaagatatcg
     2521 tcaaaggcgg tattaaagag cgcttcgccc agctgcgcaa aatgggcatt aaaacggtga
     2581 tgattaccgg cgataaccgt ctgactgccg ccgccattgc tgcggaagcg ggcgtcgatg
     2641 attttctcgc cgaagcgaca ccggaggcca agctggcatt gattcgtcag tatcaggcgg
     2701 aaggccgttt ggtggcgatg accggcgacg gcaccaacga tgctccggcg ctggcgcaag
     2761 cagatatcgc ggtggcgatg aactccggca cccaggcggc gaaagaggcg ggcaatatgg
     2821 tcgatctcga ctctaacccg accaagttga tcgaggtggt gcatattggc aaacagatgc
     2881 tgatgacccg tggctcgctg accaccttca gcattaccaa cgatgtggcg aaatacttcg
     2941 ccattattcc ggcggcgttc gcggcaacgt atccgcagtt aaatgcgctg aacatcatgc
     3001 gcctgcattc gcccgactcc gccattctca gtgcagtcat tttcaacgcc ttgattatcg
     3061 tctttttgat tcctctggcg ttaaaaggcg tgagttataa accgcttacc gcttctgcca
     3121 tgttgcgccg taacttatgg atttacggtc tgggtgggct gctggtgccg tttattggta
     3181 tcaaagtcat tgatttactg ctgaccgttt gcggtctggt gtgaggttta ccatgcgtgg
     3241 cttacgtccg gcattatcga catttatctt tctgttattg attactggcg gcgtttaccc
     3301 gctgctgacc accgtgctgg ggcaatggtg gtttccctgg caggccaacg gttcgttgat
     3361 tcgtgaaggt gatacggtgc ggggttcggc attaatcggg cagaatttta ccggcaacgg
     3421 cagaaatgcc ctataatcca caggcttctg gcgggagcaa tctggcggtc agtaaccctg
     3481 agctggataa acaaatagcc gcacgcgttg ctgcattacg ggccgctaac ccggatgcca
     3541 gtacgaacgt tccggttgaa ctggtgacgg catcggcaag cgggctggac aataatatca
     3601 ccccgcaagc ggcggcctgg cagatcccac gcatcgcgaa agcgcgtaat ctcagcgttg
     3661 aacagctcac gcagctgatc gcaaaataca accaacaacc gctggtgaaa tatatcggcc
     3721 agccggtcgt caacattgtc gaactcaatc tggcgctgga taagcttgat gaataacgaa
     3781 cccttacgtc ccgaccccga tcgtctgctg gaacaaactg ccgcgccgca tcgggggaag
     3841 ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga cctgggcgat gctggcagaa
     3901 gcccagcgac tgcgggcgca agggctggat attgtggttg gcgtggtaga aacccacggg
     3961 cgaaaagata ccgacgcaat gctggaaggg ctggctgttc tgccgccaaa acgcctggcg
     4021 taccgtgggc ggcacatctg cgagtttgac ctcgatgccg ccctcgcccg ccgtccggcg
     4081 ctgatcttaa tggacgaact ggcgcacagt aatgcgctag gttctcgtca ccctaaacgc
     4141 tggcaggaca tcgaagagct gctggaagca ggcattgatg ttttcactac cgtcaacgtt
     4201 cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca ccggaattca ggtacgggaa
     4261 accgtgcccg atcctttttt cgatgccgcc gacgacgtgg ttctggtgga cttgcccccg
     4321 gacgatctgc gccagcggct gaaagaaggc aaagtctata ttgccgggca ggcggagcgc
     4381 gccattgaac attttttccg caaaggcaat ctgatcgccc tgcgcgaact ggcactgcgc
     4441 cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc gggggcatcc tggcgaagag
     4501 aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg gccataacac cggtagtgag
     4561 aagctggtcc gcgcggcggc gcggctggcg tcacggctgg gtagcgtctg gcacgcggtg
     4621 tatgttgaaa cccctgccct gcaccgctta ccggaaaaaa aacgtcgggc aattctcagc
     4681 gccttacgtc tggcgcagga gctgggcgcg gagacggcaa cactttctga tccagcggaa
     4741 gaaaaagcag tagtgcgtta tgcccgtgaa cataatctcg gcaagattat tctcggtcgc
     4801 ccggcctcgc gccgctggtg gcgtcgggaa acgtttgctg accgactggc gcgcatcgcc
     4861 cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac ccgcccgcac gattaacaac
     4921 gcgccggata gccgctcttt taaagacaag tggcgggtac agattcaggg atgcgtggtt
     4981 gccgccgcgt tatgcgccgt tatcacttta attgccatgc agtggctgat ggcgtttgat
     5041 gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg tggtggcgct attttatgga
     5101 cgctggcctt cggtggttgc taccgtcatt aatgtggtga gtttcgatct cttttttatc
     5161 gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc tgctgacctt cgcagtgatg
     5221 ttaaccgtcg ggctggtgat cgggaacctt actgctggcg tgcgttatca ggcgcgggta
     5281 gcccgttacc gcgagcaacg cacacggcac ttatatgaaa tgtcgaaagc tctggcggtg
     5341 ggccgcagtc cgcaggacat cgccgccacc atcgaacaat ttattgcctc cacgtttcat
     5401 gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat tgcagccgtt aacacatccg
     5461 caaggaatga cgccatggga cgataccatc gcgcagtgga gttttgataa aggcctgcct
     5521 gcgggcgcgg gcaccgacac gttacccggt gtaccgtacc agattttgcc gctaaaaagc
     5581 ggcgagaaaa cctacgggct ggtggtggtg gagccgggga atctgcgcca gttgatgatc
     5641 ccggaacagc agcgcctgct ggagacgttt acgctgttag tcgccaatgc ccttgagcgg
     5701 ctgacgctaa ccgccagcga agaacaggcg cggatggcaa gcgaacgtga acagatccgc
     5761 aacgccctac tggcagcgct ttcgcatgat ttacgcacgc cgctcacggt gctgttcggt
     5821 caggcagaaa tcttaacgct cgatctggca agcgaaggat cgccccacgc ccgccaggcc
     5881 agcgagattc gtcagcatgt gctgaacact acccgactgg tgaataatct actggatatg
     5941 gcgcgaattc agtccggcgg ctttaatttg aagaaagagt ggttaacgct ggaagaagtg
     6001 gtcggtagcg cgctgcaaat gctggaaccg ggtttatcgt cgcccatcaa tctttctctg
     6061 ccagaaccgc tgaccttaat ccacgttgac gggccactct ttgaacgggt gctgattaat
     6121 ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg aaattggtat cgatgcccac
     6181 gttgagggcg aaaatctaca actggatgtc tgggataacg gccccggtct tccgccaggc
     6241 caggagcaga cgatatttga taagtttgct cgcgggaata aagagtcggc agtaccgggg
     6301 gtagggcttg gactggcaat ttgtcgggcg atagtggatg tacacggggg cactattacc
     6361 gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta cacttcccca gcaaactgcc
     6421 cctgaacttg aagaatttca tgaggatatg tgacaaacgt tctgattgtt gaagatgaac
     6481 aggctattcg tcgctttctg cgcacggcgc tggagggcga cgggatgcgc gtctatgagg
     6541 ccgaaacgct gcaacgcggc ttgctggaag cggctacccg taagccagat ttgattattc
     6601 tcgatctcgg cctgcccgat ggtgatggga ttgagtttat ccgcgacctg cgccagtgga
     6661 gcgcggtgcc ggtgattgtg ctttccgcac gcagcgaaga gagcgacaaa atcgccgcgc
     6721 tggatgccgg agcggatgat tatctgagta agccgtttgg cattggcgaa ttgcaggccc
     6781 gtctgcgcgt cgctttacgc cgccactccg ctaccgccgc gcccgatccg ctggtgaaat
     6841 tttccgatgt taccgtcgat ttagccgccc gcgtgattca ccggggtgag gaagaggtgc
     6901 atctcacacc aattgagttc cacctgctgg cggtgctgct caacaatgcc ggaaaagtac
     6961 tcacccagcg ccagctcctt aaccaggtgt gggggccaaa cgcggtcgaa cacagtcact
     7021 atttgcgtat ttatatggga catctgcgac aaaaactgga acaggatccc gcccgcccac
     7081 gccatttcat tactgaaacc ggtattggct atcggtttat gctttgaata ttaattttaa
     7141 tacagcctgc cttttattaa ttaaagccgt aataataaat acggcttttt atcttaaaca
     7201 acacacaaaa ataacaattc aatattttat attactgagt aaaaagctca tcattaaata
     7261 aattaagatt gatcattttt tattgatcac cttcacagtt caaccgtatt tcctggatag
     7321 aatatcttca ccttcattca catcaggaaa ggtaaattaa atggaaaata acagccgcac
     7381 tatgccccat ataaggcgga caactcatat tatgaagttt gctcatcgca atagcttcga
     7441 ctttcacttc ttcaatgccc gttagtctac cgactaaggg cacttcagcg tacaggtctt
     7501 cctgactctc tgtattacag gcatatgcct gtattccgca gtgctcattc tctgttaacc
     7561 cttgtattat cagccaccgg actggtaagg gtttctgcat tctgaaaaaa gcaatctacc
     7621 tgatttattt atcagcgggg taactttgct tttttccggc acgatcgatt tctcattcga
     7681 gaaattgagg acctgctatt acctaaaata aagagatgaa aaatgtcaaa attaaaaatt
     7741 gcggttagtg attcttgccc ggactgtttt accacgcagc gagaatgtat ctacattaat
     7801 gaaagtcgta atatcgatgt ggcggcaatc gttttatcgc tcaacgatgt tacatgcgga
     7861 aaactcgatg aaatcgatgc cacgggttat ggcatcccgg tatttattgc tactgaaaat
     7921 caagaacgtg tacccgcaga gtatttgccc tgtatttcgg gtgtctttga gaattgcgaa
     7981 tcgcgacgag aattttatgg tcgtcagtta gaaaccgctg ccagccatta tgaaactcaa
     8041 ctgcgcccac ctttcttccg cgcactggtc gattatgtca atcaaggtaa cagcgcgttt
     8101 gattgccctg gtcatcaggg cggcgaattt ttccgtcgcc atccggcggg gaatcagttt
     8161 gtggaatact ttggtgaggc gctgttccgt tccgacttgt gcaacgcaga cgtagcaatg
     8221 ggtgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg
     8281 ttcaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa caaagtggtt
     8341 ttaaacgccc tgctgacacc gggtgatctg gtgctgtttg accgcaataa ccacaaatct
     8401 aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga aaccgcacgt
     8461 aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt
     8521 gagctgatcg cggaagtcgc accgcagcgg acaaaagagg ctcgtccttt ccgcctcgcc
     8581 gttattcagc taggcaccta cgacggtacg atttataacg cccgccaggt ggtggataaa
     8641 attggtcatc tgtgtgacta cattctgttt gactccgcat gggtcggcta tgagcaattt
     8701 atcccgatga tggctgactg ttcaccgctg ttgctggatc ttaatgagaa cgatccgggc
     8761 attctggtta cgcaatctgt gcataaacag caggctggtt tttctcaaac ttcacaaatc
     8821 cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
     8881 aacgccttta tgatgcatgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
     8941 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta
     9001 aatggcatta atgcccgcaa gctgatcctc gataactgtc agcatattcg tccgttcgta
     9061 cctgaactag tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
     9121 ctgcgcttct tccagtttgt accaggggaa cactggcact cttttgaagg ctatgcagag
     9181 aatcaatact ttgtcgatcc atgcaaactg ttgctgacaa ccccaggtat tgatgcacgt
     9241 aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgcgaa
     9301 aatggcgtag tgccggaaaa atgcgatctt aactccatcc tcttcctgct gactccggca
     9361 gaagatatgg ccaaacttca gcaacttgtt gccctgctgg tacgcttcga aaaactgctt
     9421 gagtccgacg cgccattagc agaagtgctg ccttccatct acaaacagca tgaagagcgc
     9481 tacgccggtt atacccttcg tcagttatgc caggaaatgc atgatttgta tgcccgtcac
     9541 aacgtg
//
LOCUS       DAJTYD010000149         9382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTYD010000149 DAJTYD010000000
VERSION     DAJTYD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.149"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..660)
                     /locus_tag="OO585_003193"
     CDS             complement(<1..660)
                     /locus_tag="OO585_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6630687.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LP"
     gene            1002..1415
                     /gene="msrB"
                     /locus_tag="OO585_003194"
     CDS             1002..1415
                     /gene="msrB"
                     /locus_tag="OO585_003194"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6630688.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1457..1729
                     /locus_tag="OO585_003195"
     CDS             1457..1729
                     /locus_tag="OO585_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6630689.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(1890..2531)
                     /gene="pncA"
                     /locus_tag="OO585_003196"
     CDS             complement(1890..2531)
                     /gene="pncA"
                     /locus_tag="OO585_003196"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6630690.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2542..3558)
                     /gene="ansA"
                     /locus_tag="OO585_003197"
     CDS             complement(2542..3558)
                     /gene="ansA"
                     /locus_tag="OO585_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6630691.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(3725..5581)
                     /gene="sppA"
                     /locus_tag="OO585_003198"
     CDS             complement(3725..5581)
                     /gene="sppA"
                     /locus_tag="OO585_003198"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6630692.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            5742..6293
                     /locus_tag="OO585_003199"
     CDS             5742..6293
                     /locus_tag="OO585_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6630693.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAILEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6410..7453
                     /gene="selD"
                     /locus_tag="OO585_003200"
     CDS             6410..7453
                     /gene="selD"
                     /locus_tag="OO585_003200"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6630694.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQACVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7458..>9382
                     /gene="topB"
                     /locus_tag="OO585_003201"
     CDS             7458..>9382
                     /gene="topB"
                     /locus_tag="OO585_003201"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6630695.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSP"
BASE COUNT         2338 a         2342 c         2501 g         2201 t
ORIGIN      
        1 tggcagtact ttacctacag ctttagcagc accggtagag gacgggatga tgttctggga
       61 agcgccgcgg ccgccgcgcc agtctttgtg agacgggcca tcaacggttt tctgagtagc
      121 ggtagtagcg tgaacggtgg tcatcagacc ttcgatgatg ccgaagttat cgttgataac
      181 tttagccagc ggagccaggc agttggtggt gcaggaagcg ttggaaacga tgtcctggcc
      241 agcatatttg tcgaagttag cgcctttaac gaacatcgga gtgttgtctt tagacggacc
      301 agtcataacc actttcttcg caccagcggt gatgtgttta cgagcagttt cgtcagtcag
      361 gaacagacca gttgcttcag cgacaacgtc aacaccaact tcgtcccatt tcaggttagc
      421 cggatcacgt tcagcggtaa cacggatttt tttaccgtta acgatcagat gaccgtcttt
      481 cacttcaacg gtaccgtcga aacggccgtg agtggagtca tatttcagca tgtatgccat
      541 gtaatcagcg tctaacaggt cgttgattgc aacgatctcg atgtcagaac gtttctgagc
      601 agcacggaaa acaatgcgac cgatacggcc aaaaccgttg atacctactt tgatagtcat
      661 atattccacc agctatttgt tagtgaataa aaggttgcct gtaaaattac aaaaacctta
      721 cgcagcgtca agcggaatcg tgtcaatcat tgcgacaaat caatcctgtg cctaagcatt
      781 acgcgactga ctcgcctcac tcttcctttg ggcttgaaac cacatggggt cggcgccccg
      841 aattttaaag ggcaattacg ataaaaatgt gattttcatc acgatttagg tgcagccatt
      901 tcgtcgagat caagtttagg acaaaatttt gccagttggt gttaatgttt tgttagaatc
      961 agtcacgtaa tgtgagcacg attaaagtga gatgtgagca aatggctaat aaaccttcgg
     1021 cagaagaact gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag aatcatggga
     1081 cagagccgcc atttacgggg cgtttactgc ataacaagcg tgacggcgta tatcactgtt
     1141 tgatctgcga tgccccgctg tttcattccg aaaccaagta tgattccggc tgtggctggc
     1201 ccagtttcta cgaaccggtg agtgaagaat ccattcgtta tatcaaagac ttgtcacatg
     1261 gaatgcagcg catagaaatt cgttgcggta actgtgatgc ccatctgggg catgtctttc
     1321 ccgacgggcc gcaaccaaca ggcgaacgtt attgtgttaa ctctgcttct ttacgcttca
     1381 ctgatggcga aaacggcgaa gaaatcaacg gttgaaaaaa cgattcagca acttattcca
     1441 caggagcgga aaaattatga atcttgatga cattatcaac agcatgacgc ctgaggtata
     1501 ccagcgtttg tcgaccgccg ttgaactggg gaaatggcct gatggcgttg cgttgaccga
     1561 ggaacaaaaa gagaattgcc tgcaactggt gatgctgtgg caagcccgcc ataataccga
     1621 agcacagcat atgacaattg acacaaatgg tcagatggtc atgaagagca aacagcagtt
     1681 aaaagaagat tttggtatct cagcaaagcc tattgctatg tttaagtgaa tgagttatct
     1741 agtatttaag cctgttatta agggagggca aatatacttg ttcggacgtc gtgttttagc
     1801 tgcaagtagg ttaaatgcaa cactgacaag tattatctgg cctacaatgt cattgaggaa
     1861 atgtaggcca gagatgaatt caactaatat tacccctgtg tctcttccca gtctgccagc
     1921 gtatatagcg ttgccccagc tgctgacatc tccataaacg cgtgtgcact gtcctggggc
     1981 tggatattca cgccacgaca accatcggta atcacgttca ccttataacc taactgtaac
     2041 gcgtccagca cggtaaactt cacgcaatag tcagtagcca ggcccataac gatcaattca
     2101 tcgatttcat gatcgcgtaa ccagtcatcg agagaggttt tctgccgacg gccgttatca
     2161 aaaaaggcac tgtaactgtc aactaaagga ttttcgcctt tatggaacac cgctgcgatc
     2221 gctttttggt tcagtaacgg atgtaattgt gcgccttcac tgttctgcac acagtgatct
     2281 ggccagaagg tttgtggcaa accgtcgagt tggcctggcg tataaggctc tacaccgtgc
     2341 tgactggcaa aactgccgtg attcgccgga tgccagtcct ggctggcgat aaccgcttct
     2401 ccgcgcgact ggcaccagtc aataaggcgg ttagcaacct ccaccgtact gtcaccttcc
     2461 ggtacggcga gcgcgccacc agcacagaaa tcattttgta aatcgaccag taacagggcg
     2521 cgatggggca ttgcagtctc cttaatcatc cggcgtcagt tcgccgcgca ggttttggct
     2581 catggccttg cgaatggttt cagtatccag ttcctggctc agcaggtaat gcagtttggt
     2641 tagcgtggct tctacagtca tatctgcacc gccaataacg ccggcatggg cgagggcgtt
     2701 accggtggca taaccaccca tattcacttt accggacata cattgtgtca ggttgaccac
     2761 cacaataccg cgatcgctgg cttcttgtaa ttcctgcagg aaggctttgt tttgtggcgc
     2821 attacccacg ccataggagc gcagaatcaa tgctttcact ggttggcgca gaaaattgcg
     2881 cacgacatca gcagaaatcc ccggataaat cgtcactacg ccaattggtt gtggcgtgat
     2941 tggatgaacg attaattccc cttcaccgtg cggggcgggt ggcgtattca aacgacgaat
     3001 atggatacca gcttccagta acggaggaag gtttggagag gcaaacgcat caaaaccatc
     3061 ggcatgggct ttggtagtgc ggttgccgcg atacaatcgg ttattgaaaa agagcgttac
     3121 ttcgttgatc ggataattcg ccgcaacgta caacgcattc agcagattaa tttgtccgtc
     3181 agagcgtaac tcagccagcg ggatttgtga ccctgtcaca atgaccggtt taccgagatt
     3241 ctcgagcatg aacgacagcg cagaggcggt atacgccatc gtgtcggtac cgtgtagaat
     3301 gacaaaacca tcatagtcgt catagtgcgc tttaatatct tcagcaatat gctgccagtc
     3361 atccggcgtc atatcagaag aatccatcaa cggcgtatat tcatgaatgg tgaaatctgg
     3421 catctccggg cgatggaatt ccggcatcag cgccagttgg cgttgtagat gacctgacac
     3481 cggaatataa ccctgctcgg aacgctgcat cccgatggtc ccgcccgtgt aggcgacgta
     3541 aattgatttc ttttgcatga tattgatatg aatgatatcg aaagagcaaa agtatatacg
     3601 tgaggcagga ggagcgcacc tgatactatg cggataagga aatatggggc gagacagcgg
     3661 gaagaggatt cgtcgaggta aaaaataatg tggccgacga tctgtcggcc actcagtaca
     3721 agacttaacg cacgttggcg caggtcaggc aaaacgcata acggttttgt gggtcgttaa
     3781 acgcggccag cttgtcgctt tcacttttaa cggtagaggc taccgaggca agcggtgcag
     3841 gtaacatggc ctggaacgca tctggcaaca ttgcccggac agaaccagac atgttgtcca
     3901 tcactttgtc gaagaaggtc ggttcatcaa cgtagtattc cagatgccac tgtttcactt
     3961 ttgcgagctc tgctgctttg gcaaccgcat catcgaaatc cccgagacta tcgaccagcc
     4021 cgttagcttt tgcatcctga ccggtccaga cgtggccctg ggcgatttta tcaatttgct
     4081 ccggcgtcga atgacgcgca tcagcaacca gcgtgataaa gcgtttatag ccattctcaa
     4141 tgcttaattg catcatctgc tgcgcttccg gcggcagtgc cctggtgata gaaacatccg
     4201 ccagcggtga agttgagaca ccatcagtat gaacaccaat cgaatccaga ctattttcta
     4261 cggtggtgat cacgccgaag ataccgatag aaccggtcag ggtgctgggg ttagccacaa
     4321 tgtaattagc tggcgtggaa atccagtaac caccagatgc cgccatgccg cccatcgata
     4381 caaccacagg cttacccgct gcccgggctg ctgccagttc agcgcgaatc acttcagacg
     4441 cggtaacgct gccgcctggg ctattaacac gcaggacaat cgctttcact ttcgggtcaa
     4501 ggcgagcgtc gcggatttgt gccgcagtgg tatcaccgcc aacattcccc tgagtttcct
     4561 cgccatccat aattgcgcca ttagcgaaga cgacaccgat gctgtcaccg gtatctgccg
     4621 gcgttttcaa tgcgtaatcg taataactga tggcgcgata atttttatca gtcttactcc
     4681 agccgaactc tttggtcaga gctttttcga tttcggcact cgatgccagt gcatcgacca
     4741 gcttgttttc cagtgcatat ttcgcggtat cgccaccggt tttggttaaa ccctcaagca
     4801 acccttgcgc gctagggaat acctgctgag cagggatctg tcggttagcg gcaacagtat
     4861 tcagatagtt ttgccacagc tcaccaatcc agcggctgtc agcttcgcgg gctgccggtg
     4921 acatatcatc acgaataaac ggttcaacgg cagatttata cgtacccacg cggaacacat
     4981 gggtggaaac tttcagctta tccagcaacg atttgtagta cagaccgttg gtagcaaaac
     5041 cgtgcagatc aaccacgcct tgcggtgaca gccaaatttt attggcgaaa ctggcgagat
     5101 aatattgccc ctggctgtag ttctcgccaa cggcataaac cggtttcccg ctgtcacgaa
     5161 actctttcag ggctttgccg atgtactgca tagacggctg gtcgccgcct gcgaagtttt
     5221 tcagatccat cacgataccg gtgatattgt ggtcgtcctt cgcctggcga atggtgttga
     5281 cgatatcaaa cagtgagttt tcctgcagac gatcggaact ggcaccaagc agctggcggc
     5341 ttaatttact aaaccgctga gaactgtcgg gtttatcgac gatcacaccg gaaatgtcca
     5401 gcagcagtgc gccacgactg gccgtttctt tcgaatcacc accactgacc tgcatccaga
     5461 tccccacacc aaccagtacg aggaaaataa agaacaggtt aagtaccatt tcacggacga
     5521 aattcagcag acgccacgtc catttaaaaa atccggcaat aaatcgccaa agggttcgca
     5581 tgtattctcc caacttaagg tcacacctgt cacaggcgca atagatgtgg ttatcgtaat
     5641 gacccaaccg acacttgtca gcaggaattg cggactgtgc tgtaacaaaa tccactctcg
     5701 tgttaatttt gtgaataaat atcacgacag gagttaatca aatggatgca ctcgaactat
     5761 tgatcaatcg ccgtagcgcc tcccgcctgg ctgaacccgc gccaacgggt gaacaactgc
     5821 aaaacatcct gcgtgcgggt atgcgtgcgc cggaccataa gtccatgcaa ccgtggcatt
     5881 tttttgtgat tgaaggggaa gggcgcgagc gtttcagcgc catactggaa cagggggcga
     5941 ttgctgccgg tagtgatgac aaagctatcg acaaagcccg taatgcgccg ttccgcgcac
     6001 cgctcatcat cacggtggtg gcgaaatgcg aagaaaatca taaagtcccg cgatgggaac
     6061 aggaaatgtc tgccggatgc gcggtcatgg cgatgcaaat ggcagccgtt gcccagggat
     6121 ttggcggcat ctggcgcagt ggcgcattaa ctgaaagtcc ggtagtgcgt gaagcattcg
     6181 gttgccgtga gcaggataaa attgtcggtt ttctctacct cggcacgccg cagttgaaag
     6241 catctacgtc gattaacgtc ccggacccga cgccgtttgt aacttatttc tgataattgt
     6301 cgcgaaactg tctggattgt gagcaaaggc gcaggaattc agacactctc acttatcact
     6361 tcacggaatg agggttacca tacccggatt gcattgacag gagatgtcca tgagcgagaa
     6421 ctcgattcgt ttgacccaat acagccacgg agctggttgc ggctgtaaaa tttccccaaa
     6481 agtgttggaa accatcctgc acagtgagca ggcgaagttt gttgatccga atttgcttgt
     6541 gggtaatgag acccgcgacg atgcggcggt gtacgatctg ggcaatggca ccagcgttat
     6601 cagtaccacc gacttcttta tgccgatcgt tgataatcct ttcgattttg gccgcattgc
     6661 ggcgactaac gccatcagcg atatcttcgc gatggggggc aaaccgatta tggcgattgc
     6721 gatcctcggc tggccgatta acaaactttc cccggaaatt gcccgcgaag taaccgaagg
     6781 tggacgctat gcatgccgtc aggcgggtat tgcgctggct ggcggtcact ccatcgatgc
     6841 gccggagccg atttttggtc tggcggtaac ggggatcgta ccgaccgagc gggtgaagaa
     6901 aaacagcacc gcacaagccg gatgcaaact gttcctgacg aaaccgctgg ggatcggcgt
     6961 tcttaccacg gctgagaaaa aatcactgct gaaaccagaa catcagggac tagcgacgga
     7021 agtgatgtgc cggatgaaca tcgcaggcgc gtcctttgcc aacatcgaag gcgtaaaagc
     7081 gatgaccgac gttacgggct ttggtctgct gggccatttg agcgaaatgt gtcagggcgc
     7141 aggtgtgcag gcgtgcgttg actatgacgc gatcccaaaa ttgccggggg ttgaagagta
     7201 cattaagttg ggcgcagtgc ctggcggcac cgaacgtaac tttgccagct acggtcatct
     7261 gatgggtgaa atgccgcgtg aagtgcgcga tttgctgtgc gatccgcaaa cttctggcgg
     7321 tttgctgctg gcggtcatgc cggaagcaga aaatgaggtc aaagctacag ccgccgagtt
     7381 tggcattgaa ctgacggcaa ttggcgaact ggtgccagcg cgcggcggtc gtgccatggt
     7441 tgagattcgt taattcaatg cggttgttta ttgccgaaaa accgagtctg gcgcgcgcca
     7501 ttgctgatgt cctgcccaaa ccgcaccgga aaggcgatgg ctttatcgag tgcggtaatg
     7561 gtcaggtggt gacctggtgt atcggtcacc tgcttgagca ggcgcagcca gacgcctacg
     7621 acagccgcta tgcgcgctgg aatcttgcgg atttgccgat tgtcccggaa aagtggcaat
     7681 tacagccccg accctccgtg accaaacaac ttaacgtcat caaacgcttc ctgcatgaag
     7741 ccagcgaaat cgttcacgcc ggggacccgg atcgtgaagg gcaactgctg gtggatgaag
     7801 tgctggacta tctgcaactg gcaccggaaa agcgccagca ggtgcagcgt tgcttgataa
     7861 acgacctgaa cccgcaggcg gttgagcggg cgatcgaccg tcttcggtcc aacagtgagt
     7921 ttgtgccatt gtgcgtttct gcgctggcgc gagcgcgtgc cgactggctg tacggcatca
     7981 atatgacccg tgcgtatacc attctcggtc gcaatgccgg ttatcagggc gtactttccg
     8041 tgggacgcgt gcagacaccc gtgctcgggc tggtggtgcg ccgcgatgaa gagattgaaa
     8101 acttcgtggc gaaagacttc tttgaagtca aagcacatat cgtgacacct gccgatgagc
     8161 ggtttaccgc tatctggcaa ccgagcgaag cgtgtgaacc gtaccaggat gaagaagggc
     8221 gcttgttaca tcgtccactg gcggagcatg tggttaaccg cattagtggt caaccagcaa
     8281 ttgtcaccag ctataacgat aaacgggaat cagaatccgc gccgctgcct ttttcgcttt
     8341 cggcgttgca gattgaagcg gcaaaacgct ttggtctgag cgcgcagaac gtgcttgata
     8401 tctgccagaa gctgtacgaa acgcacaagc taatcactta tccgcgttcc gattgtcgct
     8461 atttgccaga agaacatttt gccggacgcc acgcggtgat gaatgccatc agcgttcatg
     8521 caccagatct gttgccgcaa ccagtggtag atccagatat acgcaaccgc tgttgggatg
     8581 acaaaaaggt cgatgcacac cacgccatca tcccgaccgc acggagttct gcgatcaacc
     8641 tgacggagaa cgaagcgaag gtctataacc tgattgcccg tcagtacttg atgcagttct
     8701 gcccggatgc ggtgttccgc aagtgtgtta tcgaactgga cattgccaaa ggcaaatttg
     8761 tcgctaaagc gcgttttctt gctgaagcag gctggcgcac gctgttaggc agcaaagagc
     8821 gcgatgaaga aaacgacggt acgccattgc ctgtggtggc gaaaggcgat gagttgctgt
     8881 gtgaaaaagg tgaagtggta gagcggcaaa cccagccgcc gcgccatttt accgatgcaa
     8941 cactgctttc ggcgatgacc gggatcgcgc gctttgtgca ggacaaagat ctgaaaaaga
     9001 tccttcgtgc gaccgatggt ctggggacag aagcaacccg tgccgggatt attgaactgc
     9061 tgttcaagcg tggttttctg accaaaaaag ggcgctatat ccactctacc gacgccggaa
     9121 aagcgttatt ccattcgctg ccagaaatgg cgacgcgacc ggacatgacc gcgcactggg
     9181 aatcggtgct gacgcaaatc agcgaaaagc agtgtcgcta tcaggacttt atgcagccgc
     9241 tggtggggac gctatatcag cttattgatc aagccaaacg tacgccggtg cggcagtttc
     9301 gcggcattgt ggctccgggc agtggtggca gtgctgataa gaaaaaggct gcaccgcgta
     9361 aacgtagtgc gaaaaaaagt cc
//
LOCUS       DAJTYD010000150         9378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTYD010000150 DAJTYD010000000
VERSION     DAJTYD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.150"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(45..1328)
                     /gene="aroA"
                     /locus_tag="OO585_003202"
     CDS             complement(45..1328)
                     /gene="aroA"
                     /locus_tag="OO585_003202"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6630696.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1399..2487)
                     /gene="serC"
                     /locus_tag="OO585_003203"
     CDS             complement(1399..2487)
                     /gene="serC"
                     /locus_tag="OO585_003203"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6630697.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2686..3377)
                     /locus_tag="OO585_003204"
                     /pseudo
     CDS             complement(2686..3377)
                     /locus_tag="OO585_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3507..5267
                     /locus_tag="OO585_003205"
     CDS             3507..5267
                     /locus_tag="OO585_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6630698.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5673..6530
                     /gene="focA"
                     /locus_tag="OO585_003206"
     CDS             5673..6530
                     /gene="focA"
                     /locus_tag="OO585_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6630699.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6585..8867
                     /gene="pflB"
                     /locus_tag="OO585_003207"
     CDS             6585..8867
                     /gene="pflB"
                     /locus_tag="OO585_003207"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6630700.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9059..>9378
                     /locus_tag="OO585_003208"
     CDS             9059..>9378
                     /locus_tag="OO585_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HCR6630701.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDT"
BASE COUNT         2397 a         2369 c         2228 g         2384 t
ORIGIN      
        1 gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc tgcctggcta
       61 atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt gggatcaaga
      121 atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat ccggtgatca
      181 ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg aatgtaatcg
      241 tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc aaacaggcga
      301 tcggtttctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc ttttgcaaat
      361 aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc catatcaata
      421 gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat ggtcgcgccc
      481 attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg tccaataccg
      541 gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga agccgaagat
      601 gcatcgcctt cgaccaaata agtacccgga gactgataag actgtccgcc ttttacgaca
      661 aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag attgagtgtg
      721 atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt atcttccggc
      781 gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga gccatcaacg
      841 tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt ttcttgttcc
      901 aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc aatcgggcgt
      961 tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca aagagctgcc
     1021 gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc accttctgcg
     1081 tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga aagcgtatag
     1141 cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct atccagcaga
     1201 ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg gttagaaacg
     1261 ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg taacgtcagg
     1321 gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg gctgtgggga
     1381 ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga agtctgtcag
     1441 cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc cgccgaccac
     1501 acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga acaatttgtc
     1561 aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag ctttcgccac
     1621 gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg ctttttgctg
     1681 attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa agaccagacc
     1741 agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat cgttgaagat
     1801 ggaataatcc agtatcgacg gacacgcgat attcgctttg cccagcaaat cttcacgaac
     1861 gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt aaatcacgcc
     1921 ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg cggcgaccac
     1981 cacatctttg ccgaagtctg gcgtctcgtc gatggcgata ccgtcgatgg tttcattcgg
     2041 gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg gcttaaccgc
     2101 gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt attttttcgc
     2161 ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg tggttttatc
     2221 accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat ggcagaataa
     2281 taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg cttcctctgc
     2341 aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac caagaccgtt
     2401 ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca ttgccggacc
     2461 agaactaaaa ttgaagattt gagccatttc ccctcaccac gttgcgttgc tattaacccg
     2521 cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg ttttatcatt
     2581 cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgaggct tgcatcacac
     2641 aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg tcacccgttt
     2701 tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt gttttccccc
     2761 gctttcatat gaatgatttc actacaacga atacaggcat agtccgccga ctccggcacc
     2821 actttgtaac gctgcgtaca atcactgggt aaaataagta aacccggttt caccttgtca
     2881 tcttcaaaga aatagacact gatttgcccg ttggtttcga gaatcgccag acgtacctgc
     2941 cccagctgct ccacgccacg caatcgtagc tccataaaga actcaaattc cgtcatgttg
     3001 gagttattga gtttcgacca ggccagctcg ccatcttcaa taataacaac cggcttacct
     3061 tccagtagat cttccagttt ttcactgtgc gccatcaacc acattaccag gcggtataac
     3121 aacgccagag tgataaagac gataagtacc ggcaccatcg gcacatcatc ataaaacgcc
     3181 acatctcccg ccgctgaacc cagcgtgaga atgattaaca cttcaaacag cgacatctgt
     3241 cgcacaccgc gtcttccggt cattttgagg aacaaaaaaa ccagtacaaa ggtataaaga
     3301 ctacgtagtg caacttcgcc aaggaaatca aaaggcactt tatcaaatgc catacggtgg
     3361 agatcgaacg ccttcatttt tatgctctta tcgtggttta ttcgctaaaa gcatagtcaa
     3421 cagttcatcg gcatggcggc ggtgatagcg cctttgagat aaaacccgta ttattgcgcg
     3481 ctttccgtac gactaaagtg attttcatga cgcaaacatt tatccccggc aaagatgccg
     3541 ctctggaaga ttccatcgct cgcttccagc aaaaactttc agacctcggc tttcagattg
     3601 aagaggcctc ctggctgaat cctgtgccta acgtctggtc tgtacatatt cgcgacaaag
     3661 agtgcgcact gtgttttacc aacggtaaag gcgcaaccaa aaaagcggcg ctggcttctg
     3721 cacttggtga atatttcgag cgtctctcaa ccaactactt ttttgctgat ttctggctgg
     3781 gcgaaaccat cgccaacggt ccgttcgtgc attatcccaa cgaaaaatgg ttcccactga
     3841 ccgaaaatga cgatgtgcca gaaggactac tcgatgaccg tctgcgcgcg ttttatgatc
     3901 cggagaatga actgaccggc agcatgctga ttgacctaca atccggtaac gaagatcgtg
     3961 gtatttgcgg cctgccgttt acgcgtcagt ccgacaatca gaccgtttat attccgatga
     4021 atatcattgg taacctgtac gtctccaacg gtatgtccgc aggtaatacc cgcaacgaag
     4081 cacgcgttca gggattgtct gaagttttcg aacgctacgt gaaaaaccgc attattgctg
     4141 aaagcatcag cctgccagag atcccggcag acgtgctggc gcgttaccca gcggtggttg
     4201 aagcgatcga aacactggaa gcagaaggtt tcccgatctt cgcatatgat ggttcccttg
     4261 gcggccagta tccggtgatt tgcgtggtac tgttcaatcc tgctaacggt acctgctttg
     4321 cctctttcgg tgcgcatcct gattttggcg tagcactgga acgtaccgtg accgagctgc
     4381 tgcaaggtcg tggcctgaaa gatttggatg tgtttactcc gccaaccttc gatgatgaag
     4441 aagtcgctga acataccaac ctcgaaacgc actttatcga ttccagcggt ttaatctcct
     4501 gggacctgtt caagcaggat gccgattatc cgtttgtgga ctggaatttc tccggcacca
     4561 cggaagaaga gtttgctacg ctgatggcta tcttcaacaa agaagataaa gaagtttata
     4621 ttgccgatta cgagcatctg ggcgtttatg cttgccgtat tatcgtgcct ggcatgtccg
     4681 atatttatcc ggctgaagat ctgtggctcg cgaataacag tatgggcagc catttacgtg
     4741 aaacgattct ttcgctacca ggcagcgagt gggaaaaaga agattacctg aacctcatcg
     4801 agcaactgga tgaagaaggt tttgatgact ttacccgcgt gcgtgagctg ttgggtctgg
     4861 cgaccgggtc ggataacggt tggtacaccc tgcgtatcgg tgaattaaaa gccatgctgg
     4921 cgctggctgg tggcgatctg gaacaggctc tggtctggac cgaatggacg atggagttta
     4981 actcatcagt atttagtccg gaacgcgcca actattatcg ctgcctgcaa acgttgttat
     5041 tactggcaca ggaagaagat cgccagccgc tgcaatatct gaatgcgttt gttcgcatgt
     5101 acggcgcaga tgccgtggaa gccgccagtg cggcaatgag cggcgaagcg gcgttttacg
     5161 gcctgcaacc ggtagatagc gatctgcacg cgtttgctgc acatcagtcg ttgttgaagg
     5221 cctacgaaaa gctgcagcgc gccaaagcag cattctgggc aaaataaaat caaatagcct
     5281 acgcaatgta ggcttaatga ttagtctgag ttatattacg gggcgttttt ttaatgcccc
     5341 gctttacata tatttgcatt aataaaataa ttgtaattat aaggttaaat atcggtaatt
     5401 tgtatttaat aaatacgatc gatattgtta ctttattcgc ctaatgctcc cttttaatta
     5461 actattttag cggaggatgc ggaaaaaatt caactcattt gttaattttt aaaatttatt
     5521 tttatttgga taatcaaata tttactccgt atttgcataa aaaccatgcg agttacggac
     5581 ctataagcca ggcgagatat gatctatatc aatttctcat ctataatgct ttgttagtat
     5641 ctcgtcgccg acttaataaa gagagagtta gtgtgaaagc tgacaaccct tttgatcttt
     5701 tacttcctgc tgcaatggcc aaagtggccg aagaggcggg tgtctataaa gcaacgaaac
     5761 atccgcttaa gactttctat ctggcgatta ccgccggtgt tttcatctca atcgcattcg
     5821 tcttctatat cacagcaacc actggcacag gcacaatgcc cttcggcatg gcaaaactgg
     5881 ttggcggcat ttgcttctct ctggggctga ttctttgtgt tgtctgcgga gctgatctct
     5941 ttacttccac cgtgttgatt gttgttgcta aggcgagtgg gcgcatcacc tggggtcagt
     6001 tggcgaaaaa ctggctaaat gtctattttg gcaacctggt cggcgcactg ctgtttgtac
     6061 ttttaatgtg gctttccggc gagtatatga ccgcaaatgg tcaatgggga ctaaacgtcc
     6121 tacaaaccgc cgaccacaaa gtgcaccata cttttattga ggccgtctgt cttggtatcc
     6181 tggcaaacct gatggtatgt ctggcagtat ggatgagtta ttctggccgc agcctgatgg
     6241 acaaagcgtt cattatggtg ctgccggtcg cgatgtttgt tgccagcggt tttgagcaca
     6301 gtatcgcaaa catgtttatg atcccgatgg gtattgtaat ccgcgacttc gcatctccgg
     6361 aattctggac cgctgtcggt tctgcaccgg aaaatttttc tcacctgacc gtgatgaact
     6421 tcatcactga taacctgatc ccggttacga tcggtaatat tatcggcggt ggtttgttgg
     6481 ttgggttgac atactgggtc atttacctgc gtgaaaacga tcaccattaa tggttgtcga
     6541 agtacgcagt aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc gagcttaatg
     6601 aaaagttagc cacagcctgg gaaggtttta ccaaaggtga ctggcagaat gaagtaaacg
     6661 tccgtgactt cattcagaaa aactacactc cgtacgaggg tgacgagtcc ttcctagctg
     6721 gcgctactga agcgaccacc accctgtggg acaaagtaat ggaaggcgtt aaactggaaa
     6781 accgcactca cgcgccagtt gactttgaca ccgctgttgc ttccaccatc acctctcacg
     6841 acgctggcta catcaacaag cagcttgaga aaatcgttgg tctgcagact gaagctccgc
     6901 tgaaacgtgc tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc tgcaaagcgt
     6961 acaaccgcga actggatccg atgatcaaaa aaatcttcac tgaataccgt aaaactcaca
     7021 accagggcgt gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa tctggtgttc
     7081 tgaccggtct gccagatgca tatggccgtg gccgtatcat cggtgactac cgtcgcgttg
     7141 cgctgtacgg tatcgactac ctgatgaaag acaaactggc acagttcact tctctgcagg
     7201 ctgatctgga aaacggcgta aacctggaac agactatccg tctgcgcgaa gaaatcgctg
     7261 aacagcaccg cgctctgggt cagatgaaag aaatggctgc gaaatacggc tacgacatct
     7321 ctggtccggc taccaacgct caggaagcta tccagtggac ttacttcggc tacctggctg
     7381 ctgttaagtc tcagaacggt gctgcaatgt ccttcggtcg tacctccacc ttcctggatg
     7441 tgtacatcga acgtgacctg aaagctggca agatcaccga acaagaagcg caggaaatgg
     7501 ttgaccacct ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg gaatacgatg
     7561 aactgttctc tggcgacccg atctgggcaa ccgaatctat cggtggtatg ggcctcgacg
     7621 gtcgtaccct ggttaccaaa aacagcttcc gtttcctgaa caccctgtac accatgggtc
     7681 cgtctccgga accgaacatg accattctgt ggtctgaaaa actgccgctg aacttcaaga
     7741 aattcgccgc taaagtgtcc atcgacacct cttctctgca gtatgagaac gatgacctga
     7801 tgcgtccgga cttcaacaac gatgactatg ctatcgcttg ctgcgtaagc ccgatgatcg
     7861 ttggtaaaca aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc atgctgtacg
     7921 caatcaacgg cggcgttgac gaaaaactga aaatgcaggt tggtccgaag tctgaaccga
     7981 tcaaaggcga tctcctgaac tacgatgaag tgatggagcg catggatcac ttcatggact
     8041 ggctggctaa acagtacatc actgcactga acatcatcca ctacatgcac gacaagtaca
     8101 gctacgaagc ctctctgatg gcgctgcacg accgtgacgt tatccgcacc atggcgtgtg
     8161 gtatcgctgg tctgtccgtt gctgctgact ccctgtctgc aatcaaatat gcgaaagtta
     8221 aaccgattcg tgacgaagac ggtctggcta tcgacttcga aatcgaaggc gaatacccgc
     8281 agtttggtaa caatgatccg cgtgtagatg acctggctgt tgacctggta gaacgtttca
     8341 tgaagaaaat tcagaaactg cacacctacc gtgacgctat cccgactcag tctgttctga
     8401 ccatcacttc taacgttgtg tatggtaaga aaactggtaa caccccagac ggtcgtcgtg
     8461 ctggcgcgcc gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag aaaggtgctg
     8521 tagcgtctct gacttccgtt gctaaactgc cgtttgctta cgctaaagat ggtatctcct
     8581 acaccttctc tatcgttccg aacgcactgg gtaaagacga cgaagttcgt aagaccaacc
     8641 tggctggtct gatggatggt tacttccacc acgaagcatc catcgaaggt ggtcagcacc
     8701 tgaacgttaa cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaac ccggaaaaat
     8761 atccgcagct gaccatccgt gtatctggct acgcagtacg tttcaactcg ctgactaaag
     8821 aacagcagca ggacgttatt actcgtacct tcactcaatc tatgtaatta gatttgactg
     8881 aaatcgtaca gtaaaaagcg tacaataaag gctccacgaa agtggggcct tttttagcac
     8941 gagagccttt tttgtcagct atctatactt taaggtgact gccaaaacag actcgacgta
     9001 gccttcgagc tgcgcaccaa cacggcctca gatgggccac atctggagaa ataccgcaat
     9061 gtcagttatt ggtcgcattc actcctttga atcctgtgga accgtagacg gcccgggtat
     9121 tcgctttatc acctttttcc agggctgcct gatgcgctgc ctgtattgtc ataaccgcga
     9181 cacctgggac acgcatggcg gtaaagaagt taccgttgaa gatttgatga aggaagtggt
     9241 gacctatcgc cactttatga acgcttccgg cggcggcgtt accgcatccg gcggtgaagc
     9301 aatcctgcaa gctgagtttg ttcgtgactg gttccgcgcc tgcaaaaaag aaggcattca
     9361 tacctgtctg gacaccaa
//
LOCUS       DAJTYD010000151         9264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTYD010000151 DAJTYD010000000
VERSION     DAJTYD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.151"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(58..594)
                     /gene="rcdA"
                     /locus_tag="OO585_003209"
     CDS             complement(58..594)
                     /gene="rcdA"
                     /locus_tag="OO585_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6630702.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            678..1886
                     /locus_tag="OO585_003210"
     CDS             678..1886
                     /locus_tag="OO585_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6630703.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1886..2701
                     /gene="ybjI"
                     /locus_tag="OO585_003211"
     CDS             1886..2701
                     /gene="ybjI"
                     /locus_tag="OO585_003211"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6630704.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            2787..3071
                     /locus_tag="OO585_003212"
     CDS             2787..3071
                     /locus_tag="OO585_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630705.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3112..4342)
                     /gene="mdfA"
                     /locus_tag="OO585_003213"
                     /pseudo
     CDS             complement(3112..4342)
                     /gene="mdfA"
                     /locus_tag="OO585_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4627..5223
                     /gene="ybjG"
                     /locus_tag="OO585_003214"
     CDS             4627..5223
                     /gene="ybjG"
                     /locus_tag="OO585_003214"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6630706.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5281..6039
                     /gene="deoR"
                     /locus_tag="OO585_003215"
     CDS             5281..6039
                     /gene="deoR"
                     /locus_tag="OO585_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6630707.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6086..7288)
                     /gene="dacC"
                     /locus_tag="OO585_003216"
     CDS             complement(6086..7288)
                     /gene="dacC"
                     /locus_tag="OO585_003216"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6630708.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKDVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7535..8160
                     /gene="gstB"
                     /locus_tag="OO585_003217"
                     /pseudo
     CDS             7535..8160
                     /gene="gstB"
                     /locus_tag="OO585_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8157..>9264)
                     /gene="yliI"
                     /locus_tag="OO585_003218"
     CDS             complement(8157..>9264)
                     /gene="yliI"
                     /locus_tag="OO585_003218"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR6630709.1"
                     /translation="QSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPDNH
                     GMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLSYS
                     EVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLFIA
                     LGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGMAM
                     NPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQ
                     PVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTD
                     RGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2235 a         2389 c         2294 g         2346 t
ORIGIN      
        1 attgttttca ttaacccgtt tacataaatg ggttttttgt tatctaaaat ttatctacta
       61 ccctgcaacc ctctcaacca tcctcaaaat ctcctcgcgc gatagcggct tacggtcggt
      121 gacaaaatgc agcgtcatcc cctcaataaa cgcatcaagc gcgcgggcgg ttccgggttc
      181 aaaccattgt tcgagcgttt gctgactgcg ctgcatccag ttttgcatta ccgtttttaa
      241 tagcggtttt cggctagcca gcgcgtagag ctggtacatc agctccatat tatccggcgt
      301 tgcaacctgt gagctgtaga tcatatcggt gatagcctgg catgcgcctt gagcatcact
      361 aacatcgcta aaaaatgcct gatattgccg ggacataatc tcagtaaaac tgctgaacgc
      421 ctccagcaac aactcatcaa ttcctgaaaa atagtaggtc atcgatccca acggtacccc
      481 ggcaagggta gcaattttgc ggtgcgtaac agcatgtatt ccgtaaagtt tcaccgcctc
      541 cagcgtggcc tggataattt tttctcgccg ttgcggatcg ttagcgcgac gcatgtcatt
      601 tcctctgtct gcaattgtgt acaaatgtac acaaccttgc taatgttgtc ttccctcttc
      661 ttattttgac gtgatctatg accgtaaatt cttcacgtaa tgcattgaaa cgccgaacct
      721 gggcgctgtt tatgttcttc tttttgccag gcctgttaat ggcgtcctgg gcaacccgta
      781 cgcctgctat ccgcgatatt ctctctgtct cgatcgctga aatgggcggt gttctctttg
      841 gtctgtcgat cggttcgatg agcggtattc tctgctcggc gtggttagtg aaacgctttg
      901 ggacacgtaa tgtcatcctg gtcacgatgt cctgcgcatt gatcgggatg atgatattaa
      961 gtctggcact ctggctgaca tcgcccctgc tctttgccgt tggtctcggc gtctttgggg
     1021 caagttttgg ttctgcggaa gtggcgataa acgttgaagg tgccgccgtt gagcgagaaa
     1081 tgaataaaac ggttttgccg atgatgcacg gtttttatag cctgggcacg ctggcaggcg
     1141 ctggtgtcgg gatggcactg acggcctttg gcgttccggc aacggtgcac attttattgg
     1201 cggcgctggt aggtatcgcg cctatttata tcgccattca ggcaatccct gacggtacgg
     1261 gcaaaaatgc tgccgatggc acccagcatg gcgaaaaagg cattcctttt tatcgcgata
     1321 tccagttgct gttgattggt gttgtagtgc tggcgatggc ctttgccgaa ggttctgcca
     1381 acgactggtt acccttatta atggttgacg gtcacggttt tagccctact tccggctcgc
     1441 tgatttatgc cggttttacc ctggggatga ccgttggacg cttcactggc ggttggttca
     1501 tcgaccgtta cagtcgcgtt gccgtggttc gggccagtgc actaatgggg gcgttgggta
     1561 ttgggctgat tatttttgtc gatagcgcct gggtcgctgg ggtgtctgtt gtactctggg
     1621 gactgggtgc ctcgctgggc ttcccgctga ccatttctgc cgccagcgat accggccccg
     1681 atgcaccaac ccgcgtcagt gtggtagcaa cgaccggtta tctggctttc ctcgtcgggc
     1741 cgccgctgct gggctatctc ggcgaacatt atggattacg tagtgcaatg ctggttgtac
     1801 tggcgctggt tattctcgcg gctattgtcg cgaaagccgt cgccaaaccc gataccaaaa
     1861 cgcagacggc gatggagaat agttgatgag cattaaatta attgcggtag acatggatgg
     1921 tactttctta agcgatcaaa aaacctataa ccgtgagcgg tttatggctc agtatcagca
     1981 aatgaaagca caaggcattc gctttgtggt cgccagcggg aatcaatatt atcagttgat
     2041 ctctttcttc cctgaaattg ctaatgaaat tgcctttgtg gctgaaaacg gcggctgggt
     2101 agtgagtgaa ggcaaagatg tttttaatgg cgagctgtcg aaggatgcct ttactactgt
     2161 cgtggaacat ttgctgacgc gcccggaagt ggaaattatt gcctgcggaa aaaatagcgc
     2221 ctatacgctc aaaaaatatg acgatgccat gaaaacggtg gcggaaatgt attatcaccg
     2281 tctggaatac gttgataact ttgacaactt agaagatatt ttctttaagt ttggcctgaa
     2341 tctttccgat gaactgattc cacaagtaca aaaagcatta catgaggcca tcggcgatat
     2401 tatggtgcca gtccacaccg gcaacggcag catcgatctg attatccccg gcgtacataa
     2461 agccaatggc cttcgccaac tgcagaaatt atggggaata gacgacagcg aagtggtggt
     2521 ctttggcgat ggcggtaacg atattgagat gctgcgtcag gcaggcttta gttttgcaat
     2581 ggaaaatgcc ggcagcgcgg tcgtcgcagc ggcaaaatac cgggcaggct ccaataaccg
     2641 tgaaggcgta ctggatgtga tcgataaagt gcttaaacac gaagcgccat ttgaccaata
     2701 aaaacggcag cacgcccggc aacacaatac ctgaatgaag gctgcataat tcgcggaaat
     2761 taacccgctg cattatttca ggcgaaatga ttatgaaaaa ttgtctactg ttgggcgcgc
     2821 ttttaatggg ctttactggt gtggcgatgg cgcaaagtgt caccgtcgat gtgccgagtg
     2881 gatacaaagt ggttgtagtt cctgattcag tcagcgttcc acaagcggtc agcgtcgcga
     2941 ctgtgccgca aaccgtctat gtggctcctg cgccagctcc ggcttaccgc ccgcatcctt
     3001 atgctcgcca tcttgccagc gtaggtgaag ggatggttat cgaacaccag attgacgacc
     3061 atcaccatta attcgagaat gcctgatcgc acaaagcagt caggcatttt tttacccttc
     3121 gtgagaattt cccatctgtt tatcttttaa aaagataacc atcagcgaca gccacaaaat
     3181 tccgttgaca aggttgaaga gattaaactg tccgttgccc ccgttcagcc aggcatgttt
     3241 gctgatttca ataccaacgg taaagatcag catttgcagc attcccatcg ccgcagaaac
     3301 cgtaccttta ctcatatcgc tggcaaacag ggttaatcgc accagtcccg cattcgccag
     3361 accaataccg aaagcataaa tacttaaccc ggcagtcatc cataaatacg cgtgcgatga
     3421 gataaccgtt gccgcagcag cgaccaacag accaatcata atcggccagc cgcccataat
     3481 tatcagcgaa cgtacggtgc ggcacgaggt cagacgcgct aacagcaagt tacccgcaat
     3541 taacgccccg aaaataggca cttgcagcaa gccatattca tagctgctca actgctcgcc
     3601 ggtaatgatg ataatcggcg actgggcgat ccacgccagc aatggcaggc taacgaatcc
     3661 cagcgccagc gcccccgcca caaagcggcc gttcttcagc accagcttat agtcacgacc
     3721 gagttctttc agtgacagtt tctcgcctat acgcgtggcg gtttcaggca tagctcgttg
     3781 cagaccgaaa aaggagatcg ctgccaatgc ggcaaacaaa acaaacatcc cctcccaggg
     3841 cagcacatgg atccacgccg cgcccaccag cggaccaagt agcggagcaa tcagcgccac
     3901 gttcgccatc agcgcggtga tcttgataca aaccgcctct tcgaaagatt cctgaattgc
     3961 ggcgtatccc acagcgccaa tgaaacagag gcttatgccc tgcaagaagc gcaacagagt
     4021 gaattgttca atgttttgcg ccagtaatat tgccagacag gtgatgataa accacaccac
     4081 ccccgccagc atcaccggac ggcgaccaat acgatccgac agcggcccca ggagccattg
     4141 taaaaacatc ccgcccgcca gatatgcggt cattgaagta ggaacccaat caatgcccgc
     4201 ctgatattgt tccaccacgg ccaacatacc gggttgaatc atatctgccg atataggttg
     4261 aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca agcctggcac
     4321 cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc agagtttaat
     4381 aaaagcgcag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa gcacattctt
     4441 acattattgc gagtatagct acgctttctt taagttttat ttaacctatg cccgttacaa
     4501 tcacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta cccagtttaa
     4561 cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac tttgcgtcgg
     4621 gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc gacgccagac
     4681 tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat taccgtggtg
     4741 ccgctgctgg ccgcggtgct ttggttgtgg gggcttacag cacaacggca actggtgata
     4801 aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg acatcttttt
     4861 ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca tgcggcggat
     4921 gactcattcc caagcgacca cggtacggtg attttcacct ttgcactggc atttttatgc
     4981 tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat tgcctggtcg
     5041 cgcgtttatc ttggcgtcca ctggccgctg gatatgctcg gtggattgct ggcaggtatg
     5101 attggctgcc ttagtgccca gattatctgg caagcgatgg ggcataaact ctatcaacgt
     5161 ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac
     5221 tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt cagagggatt
     5281 atggaaacac gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa acgcagcgat
     5341 aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac gattcgtcgc
     5401 gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt tctggaaccg
     5461 catagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga agaaaaacgc
     5521 cgggcggcaa aactggctgc gacactggta gaacccgatc agaccctctt ttttgactgt
     5581 ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt taccgccgtt
     5641 tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg cgcatttctg
     5701 tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt tcagcaaacg
     5761 ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca tgtcagtaaa
     5821 ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat gtcgatggcg
     5881 caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtccgtcc ggcgcgcatg
     5941 ggtgacctga aacgctttga tattgtggtg agtgattgtt gcccggaaga tgagtatgtg
     6001 aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac cggatgacgt
     6061 ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac tgatggaatt
     6121 tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca ttttccatca
     6181 cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg gtcccgacaa
     6241 cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag ctcgccttca
     6301 ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc ccgagattca
     6361 cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca ggtttaattg
     6421 gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac tcattaaaac
     6481 ggatacggtc agttttcgcc cccaacacta cggagattaa acgcatatcg ccctgggtag
     6541 ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg ccatcaacat
     6601 tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg ttgaaggtga
     6661 actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat gctttaccca
     6721 gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccaggccg tgcaccgtct
     6781 ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaga ccaataaatg
     6841 actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta ccggactgga
     6901 taatcacatc tttgttcaag tctgccaccg aaacctgatc gcccggtttg aggaacatta
     6961 ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg accgtcacca
     7021 tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag ctggtcatga
     7081 ttttagtcag gctcgcggga tccagtttct catccgcatt gccttctgcc agcactttac
     7141 cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct tcaacggttt
     7201 gttcggccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac
     7261 cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt caaaaaaaat
     7321 acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa cttaaagaaa
     7381 ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc tgcttctggt
     7441 gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt
     7501 cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg gtcggaataa
     7561 ttcaaccaac gtaaaaaagt attgctgacg ctcgaagaac tggaactacc ttatgagcaa
     7621 attctcgcgg gccgtgagtt tgggataaat cacgatgctg attttctggc gatgaaccct
     7681 aacgggctgg tgccgttgtt gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac
     7741 gccattgtcc gctatctggc ggcacagtac gggcaaaaac gcctgtggat cgactcaccg
     7801 gcacgtcgtg cggaagcgga aaaatggatg gactgggcaa accagacgct cagtaatgcc
     7861 catcgaggga tcctgatggg attagtcaga acaccaccgg aagagcgcga tcaggccgcc
     7921 attgatgcca gttgcaaaga gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca
     7981 aaagtaaaat ggttctctgg cgacgagttt ggtgtgggcg atatcgctat cgcaccgttc
     8041 atctacaatt tgtttaacgt tggcctgacc tggacaccgc gtccaaatct gcaacgctgg
     8101 tatcagcaac tcactgaacg ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa
     8161 ttgcgtgggc taactttaag taattcccca ctggactcgt cggtgagaac gtataaataa
     8221 ccgtcgggtc cagtgcgaac atcacgaatt cgctgccctc tgtccgttaa aatacggcca
     8281 tcttctgtca ctttgtcgcc gttgacgctc atcacaatga catctttatc tttcagcgcg
     8341 ccaataaata atttttgctg ccactggggg aatttatcgc tgttatagaa ggccatgccg
     8401 ctcacagcgg gcgaatcttt ccagtaaaaa acaggttgct cggtcccggc gacgatctcc
     8461 cctttcgctt ccggtatctt aaagcctgaa tagttgattc cccaggttgc cagcggccag
     8521 ccgtagtttt tgcctttttg cgggatatta atttcatcac caccgcgcgg gccatgttca
     8581 ttcagccaca gtgcattact ccacggattc atcgccattc cttgcggatt acgaatgcca
     8641 taagaccaga tctcggcgcg cgcaccggat tcctttataa aaggattatc atccgggatt
     8701 tcgccctgat cggtcagacg caccagtttg ccctgtaatt tatccagatc ctgcgccgtc
     8761 gggcgctgat tgttttcgcc cagagcaata aaaagataac ctttaccgtc gaataccagc
     8821 cgcccgccaa aatggttgcc ggtagacagt tttggcatct ggcggaagac ggtgcggaag
     8881 tcggtcactt ttgagagatc atcacttaag cggccataac ccacagcagt tccggctttg
     8941 ccatcatcgc caacttcgga ataacttaac cagatgcggc gagactgagc aaaatcaggc
     9001 gctaaaacca cgtccagcag gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa
     9061 agcggcgcag ataatccttt tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg
     9121 attaacatac cgtgattatc gggtaaaaag gccagtgccc agggatggtc gagtttgtct
     9181 tgcagtactt cgacatttac cgttgcagga gccgcccaga gagcggaaga aagacaaata
     9241 aggggcacaa ggaaaaagga ttgt
//
LOCUS       DAJTYD010000152         9263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTYD010000152 DAJTYD010000000
VERSION     DAJTYD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.152"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1272)
                     /gene="tdcG"
                     /locus_tag="OO585_003219"
     CDS             complement(<1..1272)
                     /gene="tdcG"
                     /locus_tag="OO585_003219"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6630710.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKV"
     gene            complement(1344..1733)
                     /locus_tag="OO585_003220"
     CDS             complement(1344..1733)
                     /locus_tag="OO585_003220"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6630711.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1747..4041)
                     /gene="tdcE"
                     /locus_tag="OO585_003221"
     CDS             complement(1747..4041)
                     /gene="tdcE"
                     /locus_tag="OO585_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6630712.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4075..5283)
                     /gene="tdcD"
                     /locus_tag="OO585_003222"
     CDS             complement(4075..5283)
                     /gene="tdcD"
                     /locus_tag="OO585_003222"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6630713.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5309..6640)
                     /gene="tdcC"
                     /locus_tag="OO585_003223"
     CDS             complement(5309..6640)
                     /gene="tdcC"
                     /locus_tag="OO585_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6630714.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6662..7651)
                     /gene="tdcB"
                     /locus_tag="OO585_003224"
     CDS             complement(6662..7651)
                     /gene="tdcB"
                     /locus_tag="OO585_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6630715.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7750..8688)
                     /gene="tdcA"
                     /locus_tag="OO585_003225"
     CDS             complement(7750..8688)
                     /gene="tdcA"
                     /locus_tag="OO585_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6630716.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            8877..9221
                     /gene="tdcR"
                     /locus_tag="OO585_003226"
     CDS             8877..9221
                     /gene="tdcR"
                     /locus_tag="OO585_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6630717.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         2465 a         2375 c         2116 g         2307 t
ORIGIN      
        1 cactttatcg agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc gcgcggcgtt
       61 tactgctttc acggcattaa tggcattacg ttcaatgcac gggatttgta cctgtccggc
      121 aaccggatcg caggtcagcc caaggttatg ctccatcgcg atttccgccg cattgcatac
      181 ctgcgccgga ctgccgccca gtagttcagt taaccctgcc gccgccattg aacaggccac
      241 gccaatctcc ccctgacagc cgacctccgc gccagagatg gaggcgttca ttttatacag
      301 cgcgccaata gcccccgctg ccagaaaata gcgggcaatt gaccgctcgt ttaccggacg
      361 acggaactta tcgtaataag ccagcactgc cggaataatg ccgcacgcac cgttagtcgg
      421 tgccgttacc acgcgcccgc cagctgcgtt ttcttcacta accgccagcg cgtacatgtt
      481 gatccagtcg atgacattca tcggatcgtt agagatgtta tcgctagaaa ccagctgacg
      541 acgcagcgct acggcacgac gcggcacatt gagcggacca ggcagcacgc cttcagtgtt
      601 catcccgcgt tcaataccgt catgcatcac ttgccagata cgggcaaacc ctgcgtcaat
      661 ttccgctttg ctgcgcagcg ccagctcgtt gtgcatcatc aggccagata tagacaggcc
      721 gttgtaatca cacattttca gcagttcacc tgctgagtgg aaatcgtaag gtacggacgt
      781 ttcgacatcg tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga
      841 gtaataggtt ttacttaata gcgcttcctg tcccttccag gcagtgatcc gcattccgtt
      901 ctcatggcga ggcaacattt cgggatggaa gataatgttc tttgctacag gaaaatcaac
      961 aatatgcgca ccagatgcca ctggcagccg cccgctgcgc gttactaact ctataaatgc
     1021 agggatctca tcaatgacaa catcctgcgg actgtttcct gccagtccca tgataatggc
     1081 gacatccgtg gcatggcctt tgcccgtcag tgacaacgcc ccatacagat cgaccacaat
     1141 atggctcgtc gcggttaata agccgctact ttccagccga tcaataaaac tttttccggc
     1201 attcattggc cccacggtat gcgaactgga gggaccaatc ccaattttga aaatatcgaa
     1261 tgcactaatc atatccacac cctcggattg ccgttcagtg aagtggagcg gaacgacctt
     1321 gcgaccgtcc cgctcacgag gctttacgca ctacgtactg cgatagcttc aatttccagc
     1381 ttcacatctt tcggcaaacg cgcaacctgc acacagctcc gggtcgggta ggtcgcctga
     1441 tgctcatcga agaactgctt atagacttca ttgatggtgg caaaatcatt cagatcggtg
     1501 atgaacacgg tcatcttgat gatatcgccc acgctcagcc cggcagcaac cacgatcgct
     1561 ttgacgtttt cgaggcttaa acgcgcctga tcttgcacat cagccgggat ctcaccggtc
     1621 tgcgggcaaa ccggaatttg cccggaggtg aagaccatgc tgcctaaatc aacgccctga
     1681 acataagggc cgattgcgcc tggggcacgt tgcgtttcga taatcttttt catacatcct
     1741 ccggcgtcag agcgcctggg taaaagtacg tgaaataaca tcctgttgct gttcacgggt
     1801 cagtgcgttg aagcgcacgg cgtagccaga gacacggatt gtcaggttag gatatttttc
     1861 cgggtgctcg atggcatcca gcagcatttc ccgattcatt atgttgacgt tgaggtgttg
     1921 accgccttcg acatccgctt cgtggtggaa atacccatcc agcaggccga caaggttagt
     1981 tttacgtact ggatcttctt tgcccagcgc cgcaggaacg attgagaagg tgtacgagat
     2041 cccatctttg gcgtaggtga acggcagttt cgccaccgac gtcaatgagg ccacggcacc
     2101 tttgcggtca cgaccatgca tcgggttagc gcccggcgcg aacggtgttc cggcgcgacg
     2161 accgtccggc gtattaccgg ttttctggcc gtacaccacg ttagaagtga tagtcagaat
     2221 cgactgggta gggacggcgt tgcgataggt tggcagcgct ttaattttct tcataaagcg
     2281 ttcaaccagg tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccgt actgcggata
     2341 ttcaccgtcg atttcaaagt ccaccgccag gccgttttcg tcacggattg gtttcacgcg
     2401 ggcatatttg atggcagaca gggagtccgt cgccaccgac aggcccgcga tgccgcatgc
     2461 catagtgcga tagacatcac gatcgtgcag cgccatcagc gaagcttcgt agctgtactt
     2521 gtcgtgcatg tagtgaatga tattcagcgc gctgatgtac tgcaccgcca gccagtccat
     2581 gaagtgatcg aggctgtcca tcactttgtc gtaatccagc acgtcgtcca tcagcggtgc
     2641 tgttttcggc ccgacctgaa tcttcagctt ctcgtccacc ccgccgttaa ttgcgtagag
     2701 cagcgttttc gccaggttag cgcgtgcacc aaagaactgc atttgcttac caatcaccat
     2761 tgggctgacg cagcaggcaa tcgcgtaatc gtcgctgttg aagtcagtac gcatcagatc
     2821 gtcattttca tactgcaagg aagaggtgac gatcgacacc tgcgcggcat attttttgaa
     2881 ggcaatcggt aattcttccg accaaagaat ggtcaggtta ggttccggtg ccggccccat
     2941 agtgtgcagg gtgtgcaaat agcggaagga gtttttggtc accagcgtac gaccgtccag
     3001 ccccatcccg ccgatcactt ccgtcgccca gattgggtcg ccggagaaca gcgaatcaaa
     3061 ttccggtgta cgcaggaagc gtaccatacg gatcttcatg atgaagtgat cgatcagttc
     3121 ctgtgcctgc tgctcattga gtacgccagc ttcaaagtcg cgctcaatgt agatatcgag
     3181 gaacgatgcc gtgcggccca gcgacatcgc gccgccattt tgcgatttca ctgccgccag
     3241 ataagcgaag tagagccact gcaccgcttc ttgcgcattc tgcgccgggc gagagatatc
     3301 aaagccatat ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat gctctgccag
     3361 ctcctcacgc agacggatgg tggcttccag atcctcgcct ttttccagac gagactggag
     3421 atcggcaaat tgcagttcgc gttcacgtac cagataactg atgccataca gcgctacgcg
     3481 gcgatagtca ccgataatgc gcccacggcc atagccatct ggtaaaccgg tcagcacgcc
     3541 agatttacgg cagcgcagca tatccggtga gtaaacatca aatacgccct ggttatgggt
     3601 tttacgcaga tcggtaaaca gatattcaaa ttcactgtcc atttcacggc cataggcgtg
     3661 gaatgaactt ttaatcatat taatgccacc gaacgggtgt agcgcacgtt tcaacggcgc
     3721 atccgtttgc aggccaacaa ttttttccag cggctggtta atatatcccg catcatgagc
     3781 ggtaattgtg gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg cgttttcgat
     3841 acggatacct tccattactt tttcccacaa ttccgtggtg gcaggcgtcg cttcggcgag
     3901 gaaagattca tcgccttcat acggtgtata gttatgttga ataaaatcgc ggacattaat
     3961 ttcgtttttc cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca gcttatcgct
     4021 ggtatcaata tctaccttca tgaaaaataa tctctctaca atacttcaac taaattatgc
     4081 aaattctgcg ggcgcgttaa ctttgcctaa atgaatggca tccaaagcaa tcattttttc
     4141 ttcgttagtc ggaataacag cgcaaatgac acgcgcattt tcactggaaa caattcgctc
     4201 accacaggag ttagagcgat tattcatttc tgtatcaatc tctacgccta atacagccaa
     4261 atgttccatg accagacgac gaattaagct tgaattctct cctattccgc cggtgaatat
     4321 aattccatcc aggcgacgta atgaagctgc gtgtccggca atatgacggg caattcggtg
     4381 aacaaaggtt ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg ctttttccag
     4441 aacacgtaaa tccgaagaaa gaccggaaat acctaataat cccgactctt tattcactac
     4501 gcgttccaag tcacccaagc tctggttggt ttggctggcg acccaggaca tcgcaccaaa
     4561 atcgacatcg ccactgcggg tacccatcat caagccttcc agcggcgtca ttcccattga
     4621 ggtgtcaaca ctctgaccgt tgcgaactgc gcagattgac gcgccattgc caagatgcgc
     4681 cacaaccagg ccggagtcat cttccgctag attcagcaaa gaatgtgcgc gctgggaaac
     4741 atagcggtgc gacgtgccgt ggaaaccata acggcgtaca cctaactctt cataatattt
     4801 ccacggcagg ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa
     4861 taccgccacc tgagttacgc ccggaaataa ttgctgcgcc gattcaatac cacttaaatt
     4921 ggcgtaatta tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacctcatc
     4981 ggtaataatg gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa
     5041 ggccacactg tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc
     5101 ttcgtagctg tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt
     5161 ttctgagtta ataccgtcgg caatacctga cattaatact tcacagtcgc tggcatcgag
     5221 tacggaaaac ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt
     5281 catctctttt ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg
     5341 tcagcagacc aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag
     5401 acggtgcttt acggatggca tacatcggca acaggcacag cagggatgcg ataatcggtg
     5461 cgcccatggc ttcaataagg tcgaggatgt tcgggttggc gtaggcaaca acccaggtgg
     5521 agcccatgat gaagatcatg ctgatagtgt tcagtttacc cagcgacact ttagttttgt
     5581 cgcctttata accgaacttc agaatcagac cattcaagcc ttccagcgtc cccagatagt
     5641 gaccgaagaa agatttgaag atagccacga gtgcgatgat ggaagccgca tattccagtg
     5701 taatcgcgaa cgttgttttg gtaccggtca tggacgcaaa gtggttagcc agataagaaa
     5761 gcactggaat attctgcgct ttggcttccg ccatgttggc cggagacagc gtaaacaggc
     5821 agctaaaggc aaagaacatc accactgcaa ccatcagcat gctggcacga gaaatgattt
     5881 gggaacattt acgttcggtg aagtcgcgac cgaagtcttt ctcatactct tcacgtttag
     5941 aaaccacgaa ggaagagacg attggcgaga agttaaagga gaaaaccatg atggaaatcc
     6001 ccagccagac agtgatcagg ataccgtcat gaccggttaa cgacagcgaa ccgaggtcaa
     6061 cctggtcgat aactgcagag ttccagtaag ggatcagcga cagagaaatc agcaccaggc
     6121 tggcgataaa cggccatacc aggtagctca tcactttaac catcagatcc ttaccaaacc
     6181 agatgacgaa agccatcagc agcaacagga acagcgccac aaagccgcga ttcagcggtg
     6241 caaatccgag ctggttttcc cagaacgtca taaaggtatt ggtaatagta acgccataaa
     6301 tccacagcag tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt
     6361 taccaaaatg ctcttccacc gtttccgtaa tgttgccgga agggttagag ccagaaagac
     6421 acagacgcgc cagcgcccgg tggcaataaa atgcgatggg gtatgccaat accaacatca
     6481 gaagaatcgg gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc
     6541 cgattgccgt accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt
     6601 gttttgtctg gctggataca atgctatctg aagtactcat atcctatcct caacgaatta
     6661 attaagcgtc aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg
     6721 aaataatact gacggttttt ctgttttgaa tatattggtc taatttaccg cttaataatg
     6781 cagcacatgc cagagcacct gcgccttcgg tgacgacttt attgcgctga attaaggcaa
     6841 tcatactgtt tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa
     6901 cgatttcgta agttaaatta cccgggcggg agacatcaca accatccgcc agggtgccgg
     6961 tagttcggtg cgtggttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt
     7021 ctgactgtac gccaataaca cgaatggtcg ggttaataga tttaattgcc accgcaatac
     7081 cagcaattaa accaccacca ccaattggca caatcacgtt atcgacatca tagagatctt
     7141 ccataatttc cagaccaatc gttccctggc cagcaatcac tttcggatca tcgtaaggtg
     7201 ggataaaaat acgaccttcc atttcgacaa tttcgctcac tttagcgata gtgtcgttga
     7261 agttatcacc atgcagaacg acttctgcgg agtagtcgca cgttgccgct actttggatt
     7321 ttggcgcacc ttttggcatc accactttac cgtcgatacc cagcatcgcg caggagagcg
     7381 aaaccccttg cgcatggttg cccgcagaac aggccaccac gcctttgcgt ttttccgcat
     7441 cggtcagtga acttaattta ttaaatgcgc cacgaatttt aaatgaaccc gtacgctgca
     7501 tattttcaaa cttcaggaat atttcacctt tgcaacgttc actaaaatag ttggagcgag
     7561 gcatgcctgt tttataaatt cgcccagcca gtcgttgttt cgcttcaata atatcatcaa
     7621 tagcaaccgg cagatcgtat gtaatatgca ttataaaacc tcttcgcctg aattaaatat
     7681 gtagataacc gtaaccgaca cctgcaaaac agacaggtgg attatttatg tttaaataaa
     7741 aacaaataac taaccaactt ctattaatag ccttcgtcta cacccattat aagatgaata
     7801 ctctttggct aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca
     7861 taccgcggca tattgtgcca caggtaatgt ttcttcaact ggaatagtaa taaattgatt
     7921 tgaaccaaaa ggtgacgtca tatcacaagg aattacagtt aagaaatcag cattgagaac
     7981 aagattataa attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt
     8041 tctttgtaac gtagtaagaa gctcgctgta gtaccccata ttggtttgtg gcaacaccca
     8101 ctgttcgttc ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc
     8161 taccagcaca aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc
     8221 tgcacttaac gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga
     8281 agacagttgc gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt
     8341 gatcatccct gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac
     8401 caccgcctca gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat
     8461 ggattcggaa cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt
     8521 ccgcaccact aattccacac caaaataatc ttcaatatcg ttaatgattt tactgacggc
     8581 cggttgagtt aaccctaatt cttttgcagc cgagccgata gagccacttc taatgacttc
     8641 ctgaaagact accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt
     8701 taattacctc attgacggca tgaagtgtat caaaatgaaa tgaacaggat atgtgcgacc
     8761 actcacaaat taactttcat tactttccag ggtatcgtta ttacaattaa atatctttaa
     8821 atatcaacaa gttaaaacat aaaaatcagc ataaaccctg atttttatca aaaaaaatga
     8881 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
     8941 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
     9001 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
     9061 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
     9121 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
     9181 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
     9241 cgacgaatag cattttgtgt tga
//
LOCUS       DAJTYD010000153         9260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTYD010000153 DAJTYD010000000
VERSION     DAJTYD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.153"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..321)
                     /locus_tag="OO585_003227"
     CDS             complement(<1..321)
                     /locus_tag="OO585_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6630718.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIP"
     gene            646..852
                     /locus_tag="OO585_003228"
     CDS             646..852
                     /locus_tag="OO585_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6630719.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(914..2257)
                     /locus_tag="OO585_003229"
     CDS             complement(914..2257)
                     /locus_tag="OO585_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6630720.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(2580..3218)
                     /gene="msrA"
                     /locus_tag="OO585_003230"
     CDS             complement(2580..3218)
                     /gene="msrA"
                     /locus_tag="OO585_003230"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6630721.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            3424..5157
                     /gene="tamA"
                     /locus_tag="OO585_003231"
     CDS             3424..5157
                     /gene="tamA"
                     /locus_tag="OO585_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6630722.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            5154..8924
                     /gene="tamB"
                     /locus_tag="OO585_003232"
     CDS             5154..8924
                     /gene="tamB"
                     /locus_tag="OO585_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6630723.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAQEEVVEPKIENPQPDEKPLGETLKDLFSRPVL
                     PEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDSNQG
                     IVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGPVDM
                     DLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMRTAV
                     KGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTLNGI
                     NTAKEFPEWPSKLNGLIKTSGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGNSYM
                     QWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGT
                     VEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLV
                     TLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPWSLT
                     RDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLKPFM
                     PETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLT
                     AELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIFTRG
                     EKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTL
                     AGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATP
                     NLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVH
                     VGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLF
                     SGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLR
                     GQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQVVVS
                     GYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            8927..>9260
                     /locus_tag="OO585_003233"
     CDS             8927..>9260
                     /locus_tag="OO585_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6630724.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDR"
BASE COUNT         2310 a         2269 c         2570 g         2111 t
ORIGIN      
        1 cggaattgcg tcgtcggtgt taacaatggt ggtggtctgg taacgatcgt gcggtaactt
       61 tgctgattta atcgcttcaa tcagcgtcgc gtttttctct tttgcagagg tgcgaccagc
      121 aatatgttgc agtactcgca cttttcccac taactgcgcg ctgttccagg ttttgtagct
      181 aaactgatct ttatcaagca ccaactcgcc ccgatcggta atgccaatcg gcggcactcg
      241 ctgaccggtt tcgaactgat gtgcggaagc catcatcggc aacagcaggc aggtgagtgc
      301 cagaatcttg cgtagggtca tggtgtttcc ttcttatgat atgcaggtga tccgaccact
      361 tggatctgag ttttaatcat atgtgctatt tatcgaattt cccgcaagtg tgatgccagt
      421 ttgcggtcaa gcgcacaaat catatgaaaa atgaatgctt atactgaaga ccgcgcttcg
      481 gtaaaaagat aattctgaat aattgtaacc tttaggtaaa aaaagttata cgcggtggaa
      541 acattgcccg gatagtctat agtcactaag cattaaaatt tgcgtctcat aatagttggg
      601 ccgattgtgg caccgcacag gcgtaatact cagcaggaga taacaatgaa aattttccaa
      661 cggtacaacc cacttcaggt ggcgaagtac gtaaagatcc tgttccgtgg acggttatac
      721 atcaaggacg ttggcgcttt tgaatttgat aagggtaaga ttcttatccc aaaagtgaag
      781 gataagctgc atttgtcagt gatgtccgaa gttaaccgtc aggttatgcg tctgcaaaca
      841 gagatggctt aaccaaagtg ctatgcagta ataagacggc tcctgattca ggagccgttg
      901 atgtttctgg gggttacgcg acgctttctt ctttatcttt agcgtcaggc agttttggcg
      961 aaagggcggt ggccttgctg tcgatccggg tcaccagcag ctggtcgatg cggtagttat
     1021 cgatatccac cacttcaaat ttgtagccgg cgaatttcac cgaatcggtg cgtttcggga
     1081 tcttacgcag cataaacatc ataaagccgc cgatggtttc gtagttgccc gactgcggga
     1141 actcgtcaat atccagcacg cgcatgacgt cgtcaattgg ggtgccgccg tcaatcagcc
     1201 atgagttctc atcacgggcg acaatctgct cttccagccc ctgaccgacc agatcgccca
     1261 tcagcgtggt catcacgtcg ttgagggtga tgatccccac caccagcgcg tactcgttca
     1321 tgatcaccgc gaagtcttca cctgcggttt taaaactttc caacgcctct gaaagggtta
     1381 acgtatccgg cacaatcagc gtgttgcgaa tttgtacgcc gctgttcagt gccaggcttt
     1441 ggttagccag cacgcggttc agcaggtctt tagaatcgac ataaccgatg atgtggtcaa
     1501 tatcttcatt acagacgagg aacttagagt gcggatgttc cgccacctta ttcttcaggc
     1561 tttgctcatc ttcgtggaga tcaaaccaaa tcacgttttc acgcggtgtc attgaagacg
     1621 gaacggtacg ggattccagc tcaaagacgt tttcaatcag ctcgtgttcc tgtttacgta
     1681 acacgcccgc cagcgcaccg gcttccacta ccgcgtagat gtcatcagaa gtgatgtcat
     1741 ctttacgtac cattggcagt ttgaaaatac ggaagatcat gttcgccagg ccgttgaaga
     1801 accacaccag cggggtgcaa acgtacaggc agaagcgcat cgggttgatg atacgcaaag
     1861 ccaccgcttc tggcgcaatc ataccgatgc gtttcggggt taaatccgca aacaggataa
     1921 acatgccagt cactaacgag aaagagagaa taaagctcag ttgctcagag agctctgccg
     1981 acatatagcg ggagaacagg ctgtgaaaag ctggagaaaa tgccgcatca ccgacgatac
     2041 cgccgagaat cgccactgcg ttcagaccga tttggaccac ggtaaagaac atgccgggat
     2101 tttcctgcat attcagaacg cgttgggcat ttatattgcc ttcatcagcc agcagtttaa
     2161 gtttgatttt gcgtgaggcg gcaagcgaga tctcggacat cgagaagaac gcacttacag
     2221 cgatcaagca gagtatgact aaaatactgt ttaacatatc ttatccgacc tttcaggcca
     2281 gatcctcgga agggaaagtt gattaatttg tgtgaaatac acattgaaag ccgattcgaa
     2341 gaaagtgaac cggaattttc agtggctagt atagcgtaag gtactgtaaa gtcgccagag
     2401 ggttaaaatt cagactgctg ataaatgcaa tactgcctga tgcgctatgc cgatcaggcc
     2461 tacgcagtcc ttgcaatata ttgaatttgg acgattttgt aggcctggta agtcgtttaa
     2521 gccgcatcag gcatagacaa agagcacttt atcaacaaac tacatttgta cccgtaacgc
     2581 tatgcttccg gcggcagaca gacgccaatt ccgccaattc cacagtaacc atatgggttt
     2641 ttatgcagat attgctggtg gtcatcttcg gcataataaa acggtgtggc gttagcgatt
     2701 tccgtggtga tgtgacgatc gtcatcggcg gcaagcatcg ccgcctgaaa acgttccaga
     2761 ctggcgcgag ctgcggcatc ctgttctggg gtcagcggat aaatcgctga acgatactgc
     2821 gtgccgtggt cattgccctg acgcattccc tgggcgggat cgtgattctc ccaaaatacc
     2881 tgtagcaact gctcatagct gatgacggaa ggatcgtaaa caatgcgtac cgcttcggca
     2941 tgacccgtat caccggagca cacttcccga taagtcggat ttggcgtata gccgccggta
     3001 tagcctgcgg cggtgctgta aacgccgggt aactgccaga acagacgctc cacaccccag
     3061 aaacaaccca tcgcaaaaat ggcaatctcc attccgtcag gttcattggt cattgagtga
     3121 ccgttgaccg catgcagcgt ggctacgggc atcggggtgt tacgtccagg cagggcatcg
     3181 gcgggggaaa ccagatgctt tttatcaaat aaactcatgg tgtcgctctc ccgaaaatcg
     3241 gtcattgggg ttaaggttgt aacaagaggc gtatttgcac acaataacca ccgtgaatag
     3301 gtctaaagta aaacataaga aatatttggg gtttagtctg ctttttaatc catattactg
     3361 gatttttgtt aagccgttta acggcgttcc aggggcagga aaaaaggata ttcaggagaa
     3421 aatgtgcgct atatccgaca gttatgctgt gtaagcttac tctgcttaag cggatctgcc
     3481 gtcgccgcga acgtccgtct acaggtcgag gggttatcgg gacagctgga aaagaacgtt
     3541 cgtgcgcagc tttctacgat tgaaagtgat gaagtgacgc cagaccgccg ctttcgcgca
     3601 cgcgtcgatg atgccatccg tgaaggtctg aaagcgctgg gttattacca gccgaccatt
     3661 gaatttgatc tccgtccacc gccaaagaaa gggcggcagg tattgatcgc caaagtcacg
     3721 ccaggtgtgc cggtgttaat tggcggcacc gatgtggtat tgcgcggcgg cgcgcggacc
     3781 gataaagact atttgaaatt gctcgatact cgcccggcta ttggtacggt actgaaccag
     3841 ggcgattatg aaaatttcaa aaagtcctta accagtattg cgttgcgtaa aggttatttc
     3901 gatagcgaat ttaccaaagc gcagctgggc attgcgctcg gcctgcataa agccttctgg
     3961 gatattgatt ataacagtgg cgaacgttac cgctttgggc atgtgacctt tgaaggatca
     4021 caaatccgcg atgaatacct gcaaaatctg gtgccgttta aagagggcga tgagtatgaa
     4081 tcgaaagatc tggcggaact gaaccgccga ctttctgcta ccggctggtt taactcggtg
     4141 gtggtggctc cacaatttga taaagcgcgc gaaacgaaag tattaccatt gacgggcgtg
     4201 gtttcgccgc gaacagaaaa caccatcgaa accggggtcg gttactctac ggacgtggga
     4261 ccgcgcgtga aagcgacgtg gaaaaagccg tggatgaact catacggtca cagtttgacc
     4321 accagtacca gtatttccgc gccggaacag accctcgact tcagctataa aatgccgctg
     4381 ctgaagaatc cactggaaca atattatttg gtgcagggcg gttttaagcg cactgacctg
     4441 aacgataccg aatctgactc cactacgctg gtggcttctc gctactggga tctctccagc
     4501 ggttggcagc gtgccattaa cctgcgctgg agtctcgacc actttactca gggtgaaatt
     4561 accaacacca cgatgctgtt ttatcctggg gtgatgatta gccgcacgcg ttcttgtggt
     4621 ggcctgatgc caacctgggg cgactcgcaa cgctactcta tcgactactc caacacggcc
     4681 tggggctcag atgtcgattt ctccgttttc caggcgcaga acgtctggat ccgcacactg
     4741 tacgatcgcc atcgtttcgt tacacgcggc acgctgggct ggattgaaac cggtgatttc
     4801 gacaaagtac cgccggatct gcgttttttc gccgggggcg atcgcagtat tcgcggctac
     4861 aaatacaaat ctatcgcccc gaaatacgcc aacggtgacc tgaaaggggc ctcgaagttg
     4921 ataaccggat cgctggaata ccagtacaac gtgaccggaa aatggtgggg cgcggtgttt
     4981 gtcgatagtg gcgaagcggt aagcgatatt cgccgcagcg actttaaaac cggtaccggg
     5041 gtcggcgtgc gctgggaatc gccggtcggg ccaatcaaac tcgattttgc cgtaccggtc
     5101 gcggataaag acgaacacgg gttacagttt tacatcggtc tggggccaga attatgagtt
     5161 tatggaaaaa aatcagcctc ggcgtggtta tcgttatctt actgttgctg ggatcggtgg
     5221 cgtttctggt gggcaccacc agcggcctgc atcttgtatt taaagcggcg gatcgctggg
     5281 tgccaggact ggatattggc aaggtcaccg gcggctggcg cgatctcacc ttgtctgacg
     5341 ttcgttatga gcagccaggc gtggcggtaa aagcgggtaa tctgcatctg gcggtcggtc
     5401 ttgagtgcct gtggaacagt agcgtttgta ttaatgacct ggcgctgaaa gacattcagg
     5461 tcaacatcga cagtaaaaaa atgcctcctt ctgaacaggt tgaagaagag gaagatagcg
     5521 gtccgctgga tctctccacg ccgtatccca tcaccctgac acgggtggcg ctggacaacg
     5581 tcaacatcaa gattgatgac accacggtgt cggtgatgga cttcacctcc ggcctgaact
     5641 ggcaggagaa aaccctgacc ctgaaaccga cgtcgctgaa aggcctgctg attgctctgc
     5701 cgaaagtggc gcaggaagaa gtggtcgaac cgaaaattga aaatccgcag ccggatgaaa
     5761 agccgctcgg cgaaacgctg aaagatctct tttctcgccc ggtattgccg gaaatgaccg
     5821 acttgcattt gccgcttaac ctgaacattg aagagtttaa aggcgagcag ttacgcgtga
     5881 cgggcgacac ggacatcacc gtgcgcacca tgctgctgaa agtgagcagc attgacggca
     5941 atactaaact ggacgccctg gatatcgatt ccaaccaggg gatcgtcaac gccagcggca
     6001 cggcgcagct gtcagacaac tggccggtgg atatcaccct caacagcaca ctgaacgtgg
     6061 agccgttgaa aggtgaaaaa gtgaagctaa aagtgggcgg cgcgctgcgc gaacagctgg
     6121 agattggcgt taatctttcc ggtccggtgg atatggattt acgcgcccag acgcgactgg
     6181 cggaagccgg attgccgctc aacgtggaag tgaacagcaa acagctttac tggccgttca
     6241 ctggtgagaa gcagtatcag gcggatgatc tgaaactgaa acttaccggc aaaatgaccg
     6301 attacacgct ctctatgcgt acggcagtga agggacagga gatcccgcca gccaccatta
     6361 cccttgatgc caaaggtaat gaacagcagg tcaatctcga caaactcacc gtcgcggcgc
     6421 tggaagggaa aactgaactc aaggcgttgc tcgactggca gcaggccatt agttggcgcg
     6481 gtgagctaac gcttaatggc attaacaccg ccaaagagtt cccggagtgg ccgtcgaaac
     6541 tcaatggctt gattaaaacc agcggtagcc tgtacggcgg cacctggcag atggaggtgc
     6601 cagaactgaa gctgaccggt aacgtcaaac agaacaaagt gaacgttgac ggcacgctga
     6661 aaggcaacag ttatatgcag tggatgatcc ctgggcttca tctggaactg gggccaaaca
     6721 gtgccgaagt gaaaggcgag ctgggggtaa aagatctcaa tcttgatgcc accatcaacg
     6781 cgccggggct ggataacgcg ctgccggggc ttggcggtac agcgaaaggg ctggtgaaag
     6841 tacgcggcac ggtggaagcg ccacaactac tggcagatat caccgcgcgc ggcctgcgct
     6901 ggcaggaact ttccgtgacg caggttcgcg tggaaggcga cattaaatcc accgatcaga
     6961 tcgccgggaa actcgacgta cgcgttgagc aaatttcgca gccggatgta aatatcaacc
     7021 tcgtcaccct gaatgccaaa ggcagcgaaa agcagcacga gctacagttg cggattcagg
     7081 gcgagcctgt ctccgggcag cttaatctgg caggaagttt tgatcgcaaa gaagaacgct
     7141 ggaagggaac tcttagcaat acccgcttcc agacgctggt cggcccgtgg tcgctgaccc
     7201 gcgatattgc gctggattac cgcaataagg agcaaaaaat cagcatcggg ccacactgct
     7261 ggcttaaccc gaatgcggaa ctgtgcgtgc cgcaaactat cgatgcgggg gccgaagggc
     7321 gtgcggtggt gaatctcaac cgcttcgact tcgccatgct gaaaccgttt atgccagaaa
     7381 ccactcaggc cagcggtatc ttcacgggta aagcggatgt tgcctgggac accacgaaag
     7441 aggggctgcc gcagggcagt atcacccttt cggggcgtaa cgtgcaggta acgcaaaccg
     7501 tcaacgatgc ggcgctgccg gtggcgtttc agacactgaa tctgacggcg gaattgcgta
     7561 acaaccgtgc cgaattgggc tggaccatcc gcctgaccaa taacggccag tttgatggac
     7621 aggtgctggt gaccgatccg caaggccgcc gtaatcttgg tggcaacgtc aatatccgta
     7681 acttcaacct tgcgatgata aaccccatct ttactcgtgg ggaaaaagca gcggggatgg
     7741 tgagtgccaa cttgcgtctg ggtggtgatg tgcaaagccc gcagttgttt ggtcagcttc
     7801 aggttacggg tgtggatatc gacggcaact ttatgccgtt tgatatgcag ccgagccagc
     7861 ttgcggtcaa ctttaacggt atgcgctcga cgcttgccgg tacagtacgg acccagcagg
     7921 gtgaaatcta cctgaacggt gatgccgact ggagccaaat tgaaaactgg cgggcacgag
     7981 taacggcgaa aggcagtaaa gtgcggatca ccgtgccgcc gatggtacga atggatgtat
     8041 cgccagatgt tgtattcgag gctacaccaa acctgtttac cctcgatggt cgcgtggatg
     8101 tcccgtgggc gcgcatcgtg gtgcacgatc tgccggaaag cgcagtaggc gtctccagcg
     8161 atgtggtgat gcttaacgat aacctgcaac cggaagagcc gaaaacggcg tcgattccga
     8221 ttaacagtaa cctgattgtc cacgttggca acaatgtgcg cattgacgcc tttggcctga
     8281 aagcgcggct gacgggcgat cttaacgtcg ttcaggacaa acaagggctg ggcctgaacg
     8341 ggcagatcaa catccctgaa gggcgcttcc atgcctatgg tcaggatctg attgtgcgta
     8401 agggtgagct actgttctct ggtccgccag atcaaccgta tcttaatatt gaagctattc
     8461 gtaacccgga tgctacagaa gacgacgtaa tcgccggagt tcgcgtcact ggtctggcgg
     8521 acgaaccgaa agcggagatc ttctctgacc cggcgatgtc gcaacaagct gcattgtctt
     8581 atttgctacg tggacaaggg ctggagagcg atcagagcga cagtgcggca atgacctcga
     8641 tgctgattgg tctgggggtt gcgcaaagtg gccagattgt gggtaaaatc ggcgagacgt
     8701 ttggcgtaag caatttagcg ctcgacaccc agggagtagg cgactcctcc caggtagtgg
     8761 tcagcggcta tgtattgcca ggcctgcaag tgaaatacgg cgtgggtata tttgactcta
     8821 tagcaacact cacgttacgt tatcgcctga tgcctaagct atatctggaa gccgtgtctg
     8881 gtgtagacca ggcactggat ttgctctatc agttcgagtt ttagcaatgc gaatatttgt
     8941 ctacggcagt ttacgccaca aacaaggcaa cagtcactgg atgaccaatg cccagttact
     9001 gggcgatttc agtatcgata actaccagtt gtatagcctg ggccactatc caggcgcagt
     9061 tccggggaac ggaacggtac acggtgaagt ttatcgtatt gacaacgcca cgctggccga
     9121 acttgatgcc ttgcgcacca ggggcggtga atacgcgcgc cagttgattc agacgccgta
     9181 tgggagtgca tggatgtacg tttatcaacg acccgtcgag ggattaaagc taattgaaag
     9241 cggcgactgg ttagacaggg
//
LOCUS       DAJTYD010000154         9226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTYD010000154 DAJTYD010000000
VERSION     DAJTYD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.154"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..185
                     /gene="fklB"
                     /locus_tag="OO585_003234"
     CDS             <1..185
                     /gene="fklB"
                     /locus_tag="OO585_003234"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6630725.1"
                     /translation="AEFPVNGVIPGWIEALTLMPVGSKWELTIPQELAYGERGAGASI
                     PPFSTLVFEVELLEIL"
     gene            494..1906
                     /gene="cycA"
                     /locus_tag="OO585_003235"
                     /gene_synonym="dagA"
     CDS             494..1906
                     /gene="cycA"
                     /locus_tag="OO585_003235"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6630726.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(1951..2613)
                     /gene="ytfE"
                     /locus_tag="OO585_003236"
     CDS             complement(1951..2613)
                     /gene="ytfE"
                     /locus_tag="OO585_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6630727.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(2721..3686)
                     /locus_tag="OO585_003237"
     CDS             complement(2721..3686)
                     /locus_tag="OO585_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6630728.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(3794..4654)
                     /gene="qorB"
                     /locus_tag="OO585_003238"
     CDS             complement(3794..4654)
                     /gene="qorB"
                     /locus_tag="OO585_003238"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6630729.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLTDSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4743..5123
                     /locus_tag="OO585_003239"
     CDS             4743..5123
                     /locus_tag="OO585_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6630730.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5252..7195)
                     /gene="cpdB"
                     /locus_tag="OO585_003240"
     CDS             complement(5252..7195)
                     /gene="cpdB"
                     /locus_tag="OO585_003240"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6630731.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIIGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7385..8125
                     /gene="cysQ"
                     /locus_tag="OO585_003241"
     CDS             7385..8125
                     /gene="cysQ"
                     /locus_tag="OO585_003241"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6630732.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            8337..>9226
                     /locus_tag="OO585_003242"
     CDS             8337..>9226
                     /locus_tag="OO585_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630733.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKK"
BASE COUNT         2254 a         2219 c         2348 g         2405 t
ORIGIN      
        1 ccgctgaatt cccggttaac ggcgtgatcc ctggctggat tgaagcactg actctgatgc
       61 cggtaggttc taaatgggaa ctgactatcc cgcaggaact ggcatatggc gagcgcggcg
      121 caggtgcatc catccctccg ttcagcaccc tggtgtttga agtcgaactg ctggaaatcc
      181 tctaagcagc gcattctgtt cccctcgaac cagaggggag caggcattca gcaataaacc
      241 cttcagtttg ccaaacggcg ctattttgtg ttgcaaagac cccgtaagcg tgtatttttg
      301 tgagatgttt cgcgttatca ccgtgatatg acactcactt taaacataaa attaacatta
      361 gatctaaatc ttagtattca tcccgcgtat tgttacctaa tatcgatgag tcccgataca
      421 gattcgtcgt atcatagact gactaaaggc cgtagagcct gaacaacaca gacaggtaca
      481 ggaagaaaaa aacatggtag atcaggtaaa agtcgttgcc gatgatcagg ctccggctga
      541 acagtcgcta cggcgcaatc tcacaaaccg acatattcag cttattgcca ttggcggtgc
      601 cattggtacg gggttgtttc tggggtctgg caaaacgatt agccttgccg ggccgtcgat
      661 cattttcgtt tatatgatca ttggttttat gctctttttc gtgatgcggg caatggggga
      721 attgctgctt tcgaatctgg aatacaaatc ttttagtgac ttcgcttccg atttactcgg
      781 gccgtgggca ggatatttca ctggctggac ttactggttc tgctgggttg taaccggtat
      841 ggcagacgtg gtggcgatca cggcttatgc tcagttctgg ttccccgatc tctccgactg
      901 ggtcgcctcg ctggcggtga tagtgctgct gctgacgctc aaccttgcca ccgtgaaaat
      961 gttcggtgag atggagttct ggtttgcgat gatcaaaatc gtcgccatcg tgtcgctgat
     1021 tgtcgtcggc ctggtcatgg tggcgatgca ctttcagtca ccgactggtg tggaagcgtc
     1081 attcgcgcat ttgtggaatg acggcggctg gttcccgaaa ggtttaagtg gcttctttgc
     1141 cggattccag atagcggttt tcgctttcgt ggggattgag ctggtaggta caacagctgc
     1201 ggaaaccaaa gatccggaga aatcactgcc acgcgcgatt aactccattc cgatccgtat
     1261 cattatgttc tacgtcttcg cgctgattgt gattatgtcc gtgacaccgt ggagttcggt
     1321 agtcccggag aaaagcccgt tcgttgagct gtttgtgttg gtaggtttgc ctgcggctgc
     1381 cagcgtgatc aactttgtgg tgctgacctc tgcggcgtct tccgctaaca gcggcgtctt
     1441 ctctaccagc cgtatgctgt ttggtctggc gcaggaaggt gtggcaccga aagcgttcgc
     1501 taaactttct aagcgcgcag taccagcgaa agggctgacc ttctcctgca tctgcctgct
     1561 cggtggcgtg gtgatgctgt atgtgaatcc tagcgtgatt ggtgcgttca cgatgattac
     1621 aaccgtttcc gcgattctgt ttatgttcgt ctggacgatt atcctttgct cgtacctggt
     1681 gtaccgcaaa cagcgtcctc atctgcatga gaagtcgatc tacaagatgc cgctcggcaa
     1741 gctgatgtgc tgggtatgta tggcgttctt tgtgttcgtg gtcgtgttgc tgacactgga
     1801 agatgacact cgccaggcgc tgctggttac cccgctgtgg tttatcgcac tggggctggg
     1861 ctggttgttt attggtaaga agcgggctgc tgaactgcgg aaataaccgc attatcatgc
     1921 tggatggcgc aatgccatcc agcttttaga tcactcaccc gccagcgcgc gtgggaacag
     1981 tacattgttt tccagactga tgtgatccat caggtcatca atcagttcat taatgccgtt
     2041 atacatcgct ttccaggtgg tgcaggcttc tggcggcggt gtgacgttat tggtagtgtg
     2101 tttgatcact tccagcagtt cgcccgcttc atcgtgctcg ctttccatta cgctgattgg
     2161 ccccattgcc tggctgccca tgccttgttt gatcatcggg aagaggatct gctcttcttt
     2221 catcatgtgg ctggaaagct cttcatgcag catggtcagg tattttgtca gcccttttgg
     2281 cacgctcggt ttgtcggcgt gaacgcgctc gactttagtc gcttgcagga tcagctccgg
     2341 cagttgctcg cggtgacgat cgtggtagcg cacgatgata tggtcaatga tttctgccag
     2401 cggggcgcta cgccagtctt tctcaatcgg ttgttcagca agctttgcca gttcagcttc
     2461 aatgacctca acatccagtt ctttacgtgc cgccgcgcgc gccagcgtct gcttaccgcc
     2521 acagcagtaa tccatattat atttacgaaa cagagctgaa gcgcgaggaa tagagagcgc
     2581 cagttcacct aaaggttggt cgcgataagc catagctgat acctcattct taataagata
     2641 agatgtattt taaatacatc tttaaggcaa aagctataac ccttacgtag cagaggctta
     2701 tttaactcac agccagtatg tcaactttct gagacagatt cagttaaagg cttttccggt
     2761 tttgctctga ctacaatgac cacgccaacc actaacagcg cgataccgct cagcgtcatc
     2821 agcggcggca tttgctggcg tatcaaaaag gtgtacagca aacctgccag tgtttcgaaa
     2881 acgatcagcg gcccgagaat cactgtcggt aatcgctggc tggcgacgtt ccagcagagt
     2941 gcgccaaccc atgagcaaag cacggctatc gcaaccatca gactaataaa caccagcgga
     3001 cgggggccaa aaggtaggga gaagtccggc gtttgcgtat tcagccagta acaggcgacg
     3061 agatagccga tgagagaaac cggcagtgtg accagcgcct gcgccgtcgc ccacatcatc
     3121 ggatgtttgt cgggattctc ccgcagccag cgggcgttgc gcagggcata ccatgcccag
     3181 cagaccacgg aaactaacgc tagcacgatg cctgaggtat aacgtgccca gtcaaaatcg
     3241 gggagtccgt ggtttaactc agcaatattc acacacgcca ggccgatgcc aatacaaacc
     3301 agtgccgggg cgagttttcc ccacgcgagt ttgccgtcgc gctggctata aagcagattg
     3361 gcaaaaacgg gaatcaccac cggcagggtg ccgataatca tcgtggaaac aggcgcacca
     3421 gtacgttgaa tggcactggc aaggcagaag taatagatga ggttgcccat catagtgagc
     3481 atcaaggcgg taagccagtc gcgacgcgcc aactgacgca gacgcacgcg tcccagccag
     3541 gcaatgggca gcgcaattaa ccctaacgcc agataacgcc ccatcgactg caacatcgcc
     3601 gggtattccg gtacgatcaa cggcccgaca aaaataagcc cccacatcaa ccctgctaac
     3661 agggcgtaca gcacgccgct aatcattact ggcatccatt ggtctgtcag aagaaagttc
     3721 aggaagcaga catgattgct cccggcatcc cgtcattatt agagaggatg cgggaggatg
     3781 ccactttaat taactagtta ttaacattaa aaagatggct tacgctttcg gctaacgttg
     3841 tcgttgggcg gccaatcaat ttgctaagcg ttttgctgtt atcaaacaga ccacctttcg
     3901 atgcgccaac gtcagaatcc gtcagcatat ccgccagtcc gtcgggcagt ccgacgcttt
     3961 tcagtgccgc ggcgaaatcg gcttcgctca gattttgata ggtaacctgt ttgccgctct
     4021 gtttggtcag ctctgccgct aactgtgtca acgtccaggc actatcgccc gccagttcgt
     4081 aaaccttgcc ttcgtgaccg gcttcgctaa tcacgcgtgc cgcagctgcc gcataatctg
     4141 cccgcgttgc tgaggcgatt ttgccatcgc ccgccgcacc gataaatacg ccgtgttcca
     4201 gtgctgccgg ggcgctggcg aggtagtttt cgctgtacca gccgttgcgc agcagggtgt
     4261 aaacgatgcc agaatcagcc aacattttct ccgtctcaat gtgctcatcg gcgaggccga
     4321 gcggggaggt atctgcatgt agcaggctgg tataagcgat aaatttcacg ccagccgcct
     4381 ttgcggcatt aataacatta cgatgctgcg gggcacgttg acccacttcg ctggaagaga
     4441 tcagcagtag tttttccact ccctgaagtg cagatgtcag tgcggcttca tcgccgtagt
     4501 cagcctgacg cacggtaatg ccttgtgctg ccagagcctg ggcttttgcc ggattacgaa
     4561 cgatagccac tatttggctg gcaggaaccg ttttcatcaa ggattcaata atatagtgac
     4621 caagttggcc agtggcacca gtaatagcga tcatgggaag tctccatcgt tttgtcttgt
     4681 gtttaaatac gctaacacct aaacttactt ttagtaagta cgtacaaaaa ggtaagtatg
     4741 aaatgagtca ggttagcctg tcgcaacaac tgaaagaggg taatctcttt gcggaacagt
     4801 gcccgtcgcg cgaggtgttg aaacacgtca ccagccgttg gggggtgttg attctggtgg
     4861 cgctacgcga aggtactcat cgctttagcg acctgcggcg caaaatcggc ggggtgagcg
     4921 aaaagatgct tgcgcagtcg ttacaggcgt tagaacagga tggttttctt aaccgtatcg
     4981 cgtatccggt ggtgccaccg catgtggaat atagcctcac gccgttgggc gagcaggtga
     5041 gcgaaaaggt tgctgcactg gcggactgga ttgagttgaa tttgcccgag gtgttggcgg
     5101 tgcgggatga acgtgcggca taactgccgg atgcgctgag cttatctggt ctacggtgca
     5161 tggctggtag ggcggataag atgcgccagc atcgcatccg ccaataaatg ccggatgcgg
     5221 ttgataattt atagaccgaa aaagaagtgt tttacttact caaatccacc tgataaatcg
     5281 caaacccgat atcatcggtc gcgactttat tcatcggata ttgccctttc tctttaataa
     5341 acgctgcggc tttatctgac ggggaagttt cgaaacggat atccagtttc ttatcgccag
     5401 ctatcggtgc taaacgccag ttgttatctg ccgccgggtg aatttctccc gcacgtttcg
     5461 actcatcagc aatccacgct gtcagcaccg agcggttctc atccggtgaa gcaaaagcga
     5521 tatgtctgtc gcccgtaccg gcaaatttgc cgccgtaagc gcgatagtta ttggtggcaa
     5581 cgaggaacat agcgttcgga tcaatcggct tgccattaaa ggtcaggttc ttaatccgct
     5641 ctgcattggc attaatcatc tggcactcgc cgtcatagcg ggcgggttgg gtaacatcaa
     5701 tctgataatt cacaccatca ataacatcaa agttataagt gcggaaacca tcccagttga
     5761 tgagtgactg tggtttcgtg ctgttaggat caatctggtt aaactgtccg gcggagcact
     5821 ccagccactc tttcacctct ttaccgctgg ctttcactac aatcagcgta ttggggtaga
     5881 gataaagatc ggcggcatta cggaaggtca actggccttt ttccacctcc acatagcttg
     5941 ccgggtcatt tttgcgacca ccaactttaa acggtgcggc agctgaaagc accggcagtt
     6001 ttgccagatc cggatcgccc tgaatgtaat gttcgacata cgctttttgc gcgttgttca
     6061 cgacttgcac ggtcggatcg tcctgcacca gcgccagata gctatacata ttgtcggcag
     6121 atttaccgat tggcttgctg acgaactggc gtgtggcatc gtgatcggct ttgagtgttt
     6181 ctaccagctt gctgtcttcc gccgcgaggg attttttatt ggcgatgtcg taaatcggcc
     6241 gtgcttccgc tttcgcctgc gtcacctgcc atttaccgct gtcattactg agttgtaagt
     6301 cgaccacccc aagatgatcg ccccacatgc ccggcattac cgccggaaca ccattcagcg
     6361 tgcctttggt gatatcagcc tcttcgatat cagcaaaatc tttacccggg aaaacggcgt
     6421 gagcatggcc aaacataatg gcgttaacgc ccggaatttc actgaggtaa taaactgagt
     6481 tttccgccat tactttatac ggatcggcag acagcccgga atgcgccaga acgacaacga
     6541 catcggcacc tttctcgcgc atttcaggca cgtatttgcg cacggtttcg gtaatatcat
     6601 tcaccgtcac ttttccggat aaattagctt tatcccagcc tatgatttgc ggcggcacga
     6661 cgccaatata gccaatcttc agcgtctgtt tttttccgtc tttatcgacc acttcggtat
     6721 ctttaattaa atacggtgta aacattggct gtttggttct ggcgtcaatg acgttggcat
     6781 ttacataagg gaatttcgct cccgccagcg catttttcag gtaatccaga ccgtagttaa
     6841 attcatgatt gccgagtgta ccgaccgtat aatccagcgt attcagcgcc ttataaaccg
     6901 gatgaacatc acctgctttt aatcctttcg ccgacatgta atcggccagc ggactcccct
     6961 gaatcaaatc gccgttatcg accagtacgc tgtttttcac ttcattgcgg gcatcgttaa
     7021 tcaggcttgc cgtacgtacc agtccgaatt tttccgtggc ggtgtctttg taataatcga
     7081 aatccatcat gttgctatgc agatcagtgg tttccatgat acgcagatcg accgtcgctg
     7141 cattcacact ggcggcaatc agcgtggcca ggagcgttgc gctaaactta atcatcaggg
     7201 acatcctttt atcatcggga atacgaaaga aaagggagaa taaacgtctt acttatagaa
     7261 cagtgaagaa tgccacaatt ttacgctttg aaaatgatga cactatcaca gttggcgcat
     7321 tcattaacga tagggtataa gtaaaacaat aagttaacac cgctcacaga gacgaggtgg
     7381 agaaatgtta gatcaagtat gccagcttgc acggaatgca ggcgatgcca ttatgcaggt
     7441 ctacgacggg acgaaaccga tggacgtcgt cagcaaagcg gacaattctc cggtaacggc
     7501 agcggatatt gccgcacaca ccgttatcat ggacggttta cgtacgctga caccggatat
     7561 tccggtcctt tctgaagaag atcctcccgg ttgggaagtc cgtcagcact ggcagcgtta
     7621 ctggctggta gacccgctgg atggtactaa agagtttatt aaacgcaatg gcgaattcac
     7681 cgttaacatt gcgctcattg accatggcaa accgatttta ggtgtggtgt atgcgccggt
     7741 aatgaacgta atgtacagcg cggcagaagg caaggcctgg aaagaagagt gcggtgtgcg
     7801 caagcagatt caggtccgcg atgcgcgccc gccgctggtg gttatcagcc gttcccatgc
     7861 agatgcggag ctgaaagagt atctgcaaca gcttggcgaa catcagacca cgtccatcgg
     7921 ctcttcgctg aaattctgcc tggtggcgga aggacaggcg cagctgtacc cgcgcttcgg
     7981 accaacgaat atttgggaca ccgccgctgg acatgctgtg gccgcagctg ccggagcgca
     8041 cgttcacgac tggcagggta aaccgctgga ttacactccg cgcgagtcgt tcctgaatcc
     8101 tgggttcaga gtgtctattt actaaattca gatggcagaa acagtgtatt tcctgattct
     8161 gccatcctga tttctcccaa cctaaaaagt tataaataaa aagagattgt atttaaagtg
     8221 caaaaattca attgctaata agttacattt taataatgag cgttttttga tagtttactt
     8281 ctataatgag atatttaatg gcgacataaa ataaccaaat aaaataaggt agccatatgt
     8341 tacccaggat cagacacaat aattttattg gtgcggtgga gttattcgta aaatcatcgc
     8401 atacaaaaac acattcaaac aattttttta ataatattca gcatgcattt aagaaaaaag
     8461 attgggtttc gaattatgat agcctgttaa ctttgtggga gttttttcgt tgcgcaactc
     8521 agattgataa agatagttat caagtgcttt catcaaaaaa tgaaactgtt aaagccatgg
     8581 ataagtttct tatcagtttc agccttaaag ataacggtgc cgaatataca atgacattgc
     8641 gaggcagtgg atttgaatac gaagaaatcc ccataacaat aaatgagtat aactcattca
     8701 tggatttcaa aaaccgtgaa tttccgttag aacaaaacag aagattgtat gcttgggata
     8761 ttctgcagaa aaaacagtct gacataccga aaaggattaa aggttatatt caccaggcga
     8821 ttggtgacgt gtctttaggt tatgccttat tggaggatat agtaagcaaa ttaaaacggg
     8881 gtaaatttga actccaaata ccaggaggtg gaattaaaga atgcgatgga tggtatattt
     8941 acgaaaaaat aattgatgat aattttgcca tagtcataga atctttgggc tttgcgctta
     9001 aaatctatgg aggggatgaa cgttttcgta atggttcctc tgttgttttg gaggatgaag
     9061 attactctct tatttataat tttctggtta atgcaggttg ccaacaagtc gaattagccg
     9121 aacaagtgga tgcgatagta agtgcgaatc tggccgtaga tagtgatatt accgaagagg
     9181 agatttgcga gaaatataaa tcaaccattg aggcgtttaa aaaaga
//
LOCUS       DAJTYD010000155         9089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTYD010000155 DAJTYD010000000
VERSION     DAJTYD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.155"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            52..936
                     /locus_tag="OO585_003243"
     CDS             52..936
                     /locus_tag="OO585_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6630734.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO585_003244"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO585_003244"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6630735.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO585_003245"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO585_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6630736.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO585_003246"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO585_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6630737.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO585_003247"
     CDS             5160..6443
                     /locus_tag="OO585_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6630738.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO585_003248"
     CDS             6590..8065
                     /locus_tag="OO585_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6630739.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPSQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVSDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            8159..8245
                     /locus_tag="OO585_003249"
                     /pseudo
     CDS             8159..8245
                     /locus_tag="OO585_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8246..>9089
                     /locus_tag="OO585_003250"
     CDS             8246..>9089
                     /locus_tag="OO585_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6630740.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2394 a         2181 c         2106 g         2408 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tagtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgaccagg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgagtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgccct gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggatcgt atacgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaactgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat
     8281 cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga
     8341 cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta
     8401 tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca
     8461 gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa
     8521 tacccatggc ttaaacctga ctgctcataa atacccgggc ttacgcggca cactacaaac
     8581 cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca
     8641 ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga
     8701 gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa
     8761 tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta ttggtatcga
     8821 tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga
     8881 tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtgtatgttg cagggcagct
     8941 aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct tttacactca
     9001 tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg attcggggcg
     9061 tgaagtggcc aaattcagtg tgccacttc
//
LOCUS       DAJTYD010000156         9028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTYD010000156 DAJTYD010000000
VERSION     DAJTYD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.156"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..2026)
                     /gene="fdeC"
                     /locus_tag="OO585_003251"
                     /pseudo
     CDS             complement(1..2026)
                     /gene="fdeC"
                     /locus_tag="OO585_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            3142..3243
                     /locus_tag="OO585_003252"
     CDS             3142..3243
                     /locus_tag="OO585_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630741.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            3607..3870
                     /locus_tag="OO585_003253"
     CDS             3607..3870
                     /locus_tag="OO585_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR6630742.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            3870..4010
                     /gene="ykgO"
                     /locus_tag="OO585_003254"
     CDS             3870..4010
                     /gene="ykgO"
                     /locus_tag="OO585_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCR6630743.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(4045..4272)
                     /locus_tag="OO585_003255"
                     /pseudo
     CDS             complement(4045..4272)
                     /locus_tag="OO585_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5095..5637
                     /gene="ecpR"
                     /locus_tag="OO585_003256"
                     /gene_synonym="matA"
     CDS             5095..5637
                     /gene="ecpR"
                     /locus_tag="OO585_003256"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCR6630744.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINCRFLRKDIKKDKITDQEMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            5663..6298
                     /gene="ecpA"
                     /locus_tag="OO585_003257"
                     /gene_synonym="matB"
     CDS             5663..6298
                     /gene="ecpA"
                     /locus_tag="OO585_003257"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCR6630745.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            6356..7024
                     /gene="ecpB"
                     /locus_tag="OO585_003258"
                     /gene_synonym="yagY"
     CDS             6356..7024
                     /gene="ecpB"
                     /locus_tag="OO585_003258"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCR6630746.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            7050..9028
                     /gene="ecpC"
                     /locus_tag="OO585_003259"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             7050..9028
                     /gene="ecpC"
                     /locus_tag="OO585_003259"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
BASE COUNT         2442 a         2217 c         2063 g         2306 t
ORIGIN      
        1 tgtaatgtgc ccagctgata ctgctcgctg aaagcacgca cacacgcaca acaacgtttc
       61 cggcacgggt cgaagaaacc gtagcgctgt aaacgcctgg ttttatctcc gaaattgtac
      121 caatggtgac accattagtg tcttgcggaa caaatcgcaa gcggctggcc tctcccgtca
      181 ccgggttacc atcagtatcc accgcagtaa gcgtcagcgt gtgggattgc tgaccatcag
      241 caaccacatc tcctgagagt tcattcacgc tcagagtaga cttcgacaca tccatcatcg
      301 tggcgcgcaa ttctgcagtg acggttttgc tcatgccatc aacgctaaca gtaatcgttg
      361 cctcacctga ttgcgttccg gtagtgaaga cagactgata caccccagct tcagtttcag
      421 tgaactcacc cagtgttggc tttgcctgtg atttagtggc cttcagggaa cgagtcacaa
      481 tatttccagc cggtttgaaa gttagttcag tcttgatctg atctttcatg cccgtgactg
      541 gctggccctc ggcgtcgcgc agagacagca ccagcggttt ttgctcatta ccgttagcaa
      601 gcatttgaat acggctctga ccgtcaagcg ttaacgccgt gcgatcggcg ctcataccag
      661 ctccggtaat gaccacctct gtctgcacgc gttttgaggt attgcctttg ttatcgtagg
      721 caaccgcaga aatcgcataa taattgtctt tgcctggacg ataagccggg agcgttactt
      781 gccactgact accctgaccg gtaattttgc caccttcagc cagtaatgac ggcgcttccc
      841 actgcacatt tttcagtccg tgagttgctt tgctgaccac aagcccgagg gaaagtgtct
      901 gacagccctt gccttcaata cgctcaggca gagcaatacg gatcacttca gatttgcggt
      961 actcaagaac gatgttgtta ttacgctcaa ccaggtcata gcggctgcct gctagcattc
     1021 gacgctcgcg aatgctgtct gtatcgagtt gtttctccag aggttcgcca atccgataat
     1081 taacttccag gccaaagcga gtgtcattct caccactctt gccctgctta tgcccggcac
     1141 tcagggtcag aagaggcaca ggcgtgtaat tcacttcagc ggaaatcgca tgtggatctt
     1201 tctggcgttt atctttgcca aacagcccaa cttcatcgcc ataatactgc tcatacatca
     1261 ggcttgcgcc aagctgcggt caggcgggta aatagccctc agcacgaata tcccagccat
     1321 tcgccgggcg ttcctgataa tcctcaatat ccggcgattt tttccagcca gaagcccgaa
     1381 tataaccatt ggcgctcagt ttcagataat cgcgccagta ttccgcacca acaccaatgc
     1441 gggtatgact acgggataaa tcatggtcga taaaggtgtt cacccccccc attcagtcat
     1501 ttcctgaaaa atgacgccag ccaaaaccaa tatttgactg agtacgatcg tctgtacgat
     1561 gtattgcccc ctgagtgaac aacatatttg tcggcgtatc ataaatcgga taaagcattt
     1621 ccagcgaaga atccttcagc gagaaatctt tatcgacatt cagtttgacg cgcgctgtac
     1681 catatttccc gagccactcc tgtatttcct ggttagcttt agcggtggcc attccggtaa
     1741 taaaattacg tgtcgcatcg ctatctggct gactgcttaa aaatgtcccg gcatttgcgg
     1801 caaacgacgc gacatttttc tccacgttat tatcagcagt taccgtagta tttcccatgc
     1861 tcaaccgtgg ctgaaccgca tgctgcgcac gtgccgccat tactggggta aaggtgacag
     1921 cgagtggaaa aagaacctga acagagatat ttgcccacgc cacgcagcgg gccagaactg
     1981 aataacgaaa tcgtggttgt ttatgacctg ttttataacg tgacattgag ataatttcct
     2041 gatgaacgaa ttaacgaaca taggcagaca caaataatgg cgtctgcttg tcagaaaaac
     2101 cggggatttc ccggtttcct tttccgcaga aaaggaaata cctttatgta agaaagtgca
     2161 ccgccacctg atatatccag tgtctgtagc cactcatcag gtggttgggc gcgggattat
     2221 atttttattt atgggtaaaa atttagcttt ttgtgcaagt cattaaatag atacctaagt
     2281 tatatttctt actctgcgga cagtgaataa ttcattattc agactgaata ataatgacct
     2341 gcctttaatt agcaactatt ggaaatacgt cgaagtaggt atatacgcca ggataaaccg
     2401 atatatttat tatggatatt cattttcctt catcggactg taaattgcgg ggatatcgaa
     2461 cttacactac tcatcgcaac actcaaacag atttatgaat ttcgttcata acccctgaca
     2521 aatcctgccg tctaatcgag cttttccctt tacgttatgc ttctcatgaa cgagaacaca
     2581 acttattcat gaattaacca ttccggataa actatggaat caaattcagg catcgaaagg
     2641 aactggctgg tttccgttaa tagattaaat atctattact ctcccgagag taaaactatc
     2701 aaatcaatga gtaaccgata cagcctcaca aattctttca gataattaac ccgtcactgc
     2761 ggcacgttct ctcgtaaggc gtgcctgcag tgtaatccag tatgtatccg gcatagttga
     2821 cggtttaata gaactcaatt cctctccctg gtcaccaaca acgatatctt gtataacacc
     2881 ccaggagata cgacattatg atgagatgcg atatgctcct cagcaccaat caacgccagg
     2941 actgggccag tcttgagatc agactcattc ccgggactga cttcagtcga catatatcaa
     3001 tgacgttggt gcaataaaag atagccgatg taatacatct attcaaattt gtgatacatg
     3061 tcaaaaaact ggttgaccaa actcgttatt tatataaggg cacttacgaa gtgcgctctt
     3121 ttttaaagcg aggaagtacc aatgaaagag aataaagtac aacaaatcag tcataaacta
     3181 attaatatcg ttgtttttgt cgcaattgta gaatacgcct atttatttct ccatttctat
     3241 taataacggc aataaactgt tcacttcagt gatatttaaa atatgcattc tctccctttt
     3301 tgtaagtaat tattatatcc gtgggagagg aatacacgct gtcaggtaat caatcatact
     3361 gcgataaatc atcggccagt aaagtggaga taacctccat tgtcgaaaaa tccatactct
     3421 cagcgaaacc atcatcaatc actcatccag gcgtttatgg gagcgtcgcc aacggctgtt
     3481 aacaatgcca gacttccccg ttgcggaaat tccacatacc actaatggtc acagggattg
     3541 ggtgttgaaa tgatccggat gagcatgtat ctttatggtt atgctataac ataacaggta
     3601 aaaatgatga agcccaatat ccatcctgag tatcgtactg tggtgttcca cgacaccagt
     3661 gttgatgagt actttaaaat cggctcgact atcaaaacag accgtgagat tgagctggat
     3721 ggcgtaacgt atccgtacgt gacaattgat gtctcttcta aatcgcaccc gttctataca
     3781 gggaagctga gaacagtggc atcagaagga aatgttgcac gattcaccca acgttttggt
     3841 cgttttgtta gcacgaaaaa ggggtcgtga tgaaagttct taactctctg cgtaccgcaa
     3901 aagaacgcca tccagactgt cagattgtga agcgaaaagg acggctatat gtgatttgta
     3961 aatctaatcc acgttttaag gccgttcagg gtcgtaagaa aaaacgttga ttcaaaattc
     4021 gacggattaa cgatatttgt ctgattaata atcagatcgg attaatgttg gtgtgtttat
     4081 aacaccaaca ttaattttcc tggggatata ttcttcctgt tcatttgagg ccaactgcct
     4141 gacgtttctc tacgaatatt ccattatctt aatgttgact tgttgaccag cttcgcccct
     4201 gtatgctggc atcaactctc ttttagactg aacacgccac tcagtctcct ccctttgcgg
     4261 cgcagcctgc attttcactc aaactgttaa gatgataaat gtggtaaatc tgttggtact
     4321 aacataaaaa cgtttacgcc acaggaacag tctgatccac cggtaacccc gtcgccgacg
     4381 ttcgagtgcc agttagagta acgcgcacag ataactgcac tcagtgccct gacaaagagg
     4441 ccatcgttcc tgtgacagct ggcagccttc gtttaacttc acttaatctg gctcttgagg
     4501 gcttaccgaa cagatgacgt acatacgccc gttcaatttt ccatcactta ttgtaatgaa
     4561 cacctataac cattttgtgc ggcatgttaa tccattaaaa tttcttactg attggcaaat
     4621 catcttcaat gacagctcat tatagtttta tattctatcc cttaccctta aaacttgttt
     4681 ttttactagt ccatcacaca gcgcattaag actattccta acacttcagg gcaaagttcc
     4741 tgaccaatat aaaatgcaag taagaattga acgttatatt gccaataacc ttatgaaacc
     4801 aaatgtcttt ttcttcttat caaaaaaaca atattttcag tttttctaaa tattgactta
     4861 accattgaat tccttttccg ttcacatatt gacactcatc gaaaaaaaaa cataaattta
     4921 agcccaatcg aaaataatta aacttaatct cgtttaacct ttattgatat gtactacgta
     4981 tcttatttac ttccggttta ctaaggaaac tgaatgcacc tgtaaaaatt acaggtttgg
     5041 aaagtagtga catggcaaag tgattacagt agggactatg aggttaaaaa ccatatggaa
     5101 tgtcaaaacc gttctgataa atacatctgg tctccccatg acgcctactt ctataaagga
     5161 ctatctgaac tgattgtgga tatcgacaga ttaatttatc tatcgttgga gaaaattaga
     5221 aaagatttcg tgtttatcaa tctcaatacg gattctttat ctgaatttat aaaccgtgat
     5281 aatgaatggt tatccgcggt aaaggggaaa caggtcgtat tgattgcggc cagaaagtca
     5341 gaagccttag caaattattg gtactacaac agcaatatta ggggcgtggt atacgctgga
     5401 ctgagtcgtg atattagaaa agaactggcc tatgtgatta attgcaggtt cctgagaaaa
     5461 gatattaaga aagataaaat cacggaccag gaaatggaaa ttatccgcat gacggcccag
     5521 ggaatgcaac ctaaatcgat tgccagaatt gaaaattgta gtgtgaagac agtgtatacc
     5581 catcggcgta atgctgaggc caagctgtac tcaaaaatat ataagttggt tcagtaaact
     5641 ccaggcaagt tagttttaaa aaatgactca ctgggacatc acgtcctcaa ttcaactcgg
     5701 gaagaaatgc aatgaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggcat
     5761 gggtgtggcg caggctgctg acgtaacagc tcaggctgta gcgacctggt cggcaacagc
     5821 caaaaaagac accaccagta atctggttgt gacgtcactc ggtagcttgg cgttccagta
     5881 tgccgaaggc attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg ctatcgaggg
     5941 tgactcaacg gctaccgcct ttaaactgac ctcacgtctt atcaccaaca cattaactca
     6001 gttggatacc tcaggttcca cactgaatgt gggcgtggat tataacggcg cggcagtcga
     6061 aaaaactggc gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag
     6121 cccgctggct aacggttaca atgccagcaa tcgtaccacc gcacaggatg gtttcacctt
     6181 caccatcatc agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc
     6241 ggaaggcatc tggagcggcg acgttagcgt acagttcgac gcgacctgga ccagttaatc
     6301 tctctgatgt accagcaggg gtagcccccc tgctttattc ctggacggaa ttgttatgaa
     6361 aaagcacctt ctgcctctcg ctctgctgtt ttccggaata tctccggccc aggcgctgga
     6421 tgtcggcgat atatcatcgt ttatgaacag tgacagcagc acgctgagca aaacgatcaa
     6481 aaacagtacc gacagtggtc gccttatcaa tatccgtctc gaacggctct cttcaccgct
     6541 tgacgacggg caggttatct caatggacaa gccggatgag ttgctactca ctcccgccag
     6601 cttgctgcta cccgcccaag ccagcgaagt gatccgcttc ttctataagg gacccgcaga
     6661 tgaaaaagag cgctactacc gcattgtctg gtttgatcag gccctcagtg atgcgcagcg
     6721 cgataatgcc aaccgcagcg ctgtggccac tgcttccgcc cgcatcggca ccattctggt
     6781 cgtcgcccct cgtcaggcga actaccactt tcagtacgcc aacggctccc tgacaaatac
     6841 aggaaatgcg acgctgcgga tcctcgccta cggcccctgc ctgaaagccg ccaacggtaa
     6901 ggagtgtaaa gagaattact acctgatgcc gggcaagtcg cgtcgtttta cccgcgtgga
     6961 cactgcggat aacaaaggac gggttgcact ttggcagggt gataagttca ttcccgtgaa
     7021 atagatagct gtgcagatgg ataacgacaa tgcctttacg acggttctcc ccaggactga
     7081 aagcccagtt tgccttcggc atggtctttt tgttcgttca gcccgatgcc agcgctgctg
     7141 acataagtgc gcagcaaata ggtggggtga ttattccgca ggccttcagt caggcgcttc
     7201 aggacggcat gagcgtcccg ctctatattc atctcgccgg tagtcagggt cgccaggacg
     7261 atcagcgaat cggcagcgct tttatctggc tggatgatgg acagctacgc atccggaaaa
     7321 tacagctgga agagagtgaa gataacgcca gtgtcagcga acaaactcga cagcagctga
     7381 tggctctggc gaacgccccg ttcaatgagg cccttaccat cccctgactg acaacgcgca
     7441 gttggatctc agcttgcgcc aactgctgct gcagctggtg gtcaagcgcg aagcgctggg
     7501 caccgtacta cgctcacgta gcgaagacat cgggcagtcc agtgttaaca ccctcagcag
     7561 taatctgagc tataacttgg gcgtctataa caaccagttg cgtaacggcg ggagcaacac
     7621 atccagctat ctgtcgctga ataacgttac tgcgctgcgc gaacatcatg tggtgctcga
     7681 cggctcgctg tacgggatcg gtagcggtca acaggacagt gaattatata aagcgatgta
     7741 tgaacgcgat tttgccggtc accgatttgc cggtggaatg ctcgacacct ggaacttgca
     7801 gtcattaggg ccgatgaccg ccatttcagc agggaagatt tacggccttt cctggggaaa
     7861 ccaggccagc tccaccatct tcgacagcag ccagtcagcc acgccagtga tcgccttttt
     7921 accggcggcg ggcgaagtac atctcacccg tgatgggcgg ctactaagcg ttcagaactt
     7981 cactatgggc aatcatgaag tggatacccg gggtctaccg tacggtattt acgatgtgga
     8041 agttgaggtg atcgttaacg gtcgcgtgat cagcaaacgc actcaacggg tcaataagct
     8101 gtttagccgg ggcgcggcgt cggtgcacca ctggcgtggc aggtatgggg cggtagcttt
     8161 catatggatc gctggtcgga aaacgggaaa aagacgcgac cagctaaaga gagttggcta
     8221 gcaggtgcct cgacctccgg ctcattgagt acgcttagct gggcggcaac gggatatgga
     8281 tacgataatc aggcggtggg tgaaacccgt ctgacgctgc cgcttggggg ggcgatcaac
     8341 gttaacctgc aaaatatgct ggccagtgac agctcatgga gcagcatcgg cagcatcagc
     8401 gctactctac cgggaggctt tagttcgctg tgggttaacc aggaaaaaac ccgtattggc
     8461 aatcaattgc gacgtagcga tgccgacaac cgtgcaatcg gcggcacact caacctgaac
     8521 tcactgtggt cgaagctggg cacattcagc atcagctaca atgatgaccg ccgttacaac
     8581 agccattatt acacggcaga ttactatcaa aatgtctaca gcggtacctt tggttcgctt
     8641 ggcctgcggg ccggtattca gcgctataac aacggcgaca gcaacgccaa tacagggaaa
     8701 tatatcgctc tcgatctctc gctaccactg ggcaactggt ttagcgcagg gatgacccat
     8761 caaaacggct acaccatggc aaacctgtca gcacgcaaac agtttgatga agggaccatt
     8821 cgcactgttg gtgccaatct gtcacgtgcc atctccggcg ataccggtga tgacaaaacc
     8881 ctcagcggtg gggcgtatgc acagttcgac gctcgttacg ccagcggaac gctgaacgtc
     8941 aatagcgcgg cggacggcta cgtcaatacc aacttgaccg ccaatggcag cgtcggctgg
     9001 cagggtaaaa acattgctgc cagcgggc
//
LOCUS       DAJTYD010000157         8961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTYD010000157 DAJTYD010000000
VERSION     DAJTYD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.157"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            202..711
                     /locus_tag="OO585_003260"
     CDS             202..711
                     /locus_tag="OO585_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6630747.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            708..2126
                     /gene="phrB"
                     /locus_tag="OO585_003261"
     CDS             708..2126
                     /gene="phrB"
                     /locus_tag="OO585_003261"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6630748.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2168..3649)
                     /gene="dtpD"
                     /locus_tag="OO585_003262"
     CDS             complement(2168..3649)
                     /gene="dtpD"
                     /locus_tag="OO585_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6630749.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            3920..4663
                     /gene="ybgI"
                     /locus_tag="OO585_003263"
     CDS             3920..4663
                     /gene="ybgI"
                     /locus_tag="OO585_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6630750.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            4686..5342
                     /gene="pxpB"
                     /locus_tag="OO585_003264"
     CDS             4686..5342
                     /gene="pxpB"
                     /locus_tag="OO585_003264"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6630751.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5336..6268
                     /gene="pxpC"
                     /locus_tag="OO585_003265"
     CDS             5336..6268
                     /gene="pxpC"
                     /locus_tag="OO585_003265"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6630752.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6258..6992
                     /gene="pxpA"
                     /locus_tag="OO585_003266"
     CDS             6258..6992
                     /gene="pxpA"
                     /locus_tag="OO585_003266"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6630753.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            7028..7819
                     /gene="nei"
                     /locus_tag="OO585_003267"
     CDS             7028..7819
                     /gene="nei"
                     /locus_tag="OO585_003267"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6630754.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7816..8862)
                     /locus_tag="OO585_003268"
     CDS             complement(7816..8862)
                     /locus_tag="OO585_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6630755.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
BASE COUNT         2216 a         2254 c         2493 g         1998 t
ORIGIN      
        1 taattaagct ttgagaactg ttattttcta aaaataaaaa atacaaaaaa tgaatgcagc
       61 tcgggaaata gtagaataat tctcgctcag atgaagttga taatattttt accaacaaaa
      121 tacttccccg caaaaactct gccccataaa attcatttac catcgtctac acttactcct
      181 ttataccgtt caggagcagt aatgaatcta caacgatttg atgacagcac cctaatccgt
      241 atctttgccc ttcatgagtt acaccgactg aaagaacatg gcttaacgcg cggggcgctt
      301 ctcgattatc acagccgcta taaactcgtc tttctggcgc attctcagcc ggagtaccgc
      361 aaacttggcc cgttcgtggc tgatattcac cagtggcaaa atctggatga ctattacaac
      421 cagtaccgcc aacgcgtagt tgttttgctt tctcaccccg ccaacccgcg cgatcacacc
      481 aatgttttga tgcacgttca gggttatttt cgcccgcata ttgattccac agaacgccag
      541 cagctggctg cgcttatcga cagttatcgc cgtggcgagc aaccacttct tgcgccgctg
      601 atgcgtatca aacactatat ggcgctttat cctgacgcct ggctttcagg gcagcgttat
      661 ttcgaacttt ggccgcgtgt gattaacttg cgccattcag gagttttatg actacccatc
      721 tggtctggtt tcgccaggat ttacgtttgc acgataatct cgcactggct gccgcctgca
      781 gcaattcgtc tgcacgcgtg ctggcgttgt atatcgctac accacgccag tgggcgacgc
      841 ataacatgtc gccgcgtcag gctgaactta tcaatgctca actgaatggg ctacaaatag
      901 cgcttgcgga aaaaggtatt cctttattgt tccgtgaagt ggatgacttt gtcgccagtg
      961 tcgaaatagt taaacaggtg tgcgcggaaa acagcgttac ccacctgttt tataactatc
     1021 agtatgaagt gaatgagcgg gcgcgggatg tggaagttga aagagcgctg cgtaacgtgg
     1081 tgtgtgaagg atttgatgac agcgtgatcc tgccgcctgg cgcggtgatg accggtaatc
     1141 acgagatgta caaagtcttt acgcctttta agaatgcctg gctgaaacgg ctgcgggaag
     1201 ggatgccgga gtgcgtcgct gcgccaaaag ttcgtagtag cggatcgata gagccctcgc
     1261 catccattac gctgaattat cctcgtcagt ctttcgatac tgcgcatttc ccggtggaag
     1321 aaaaagcggc gattgcgcaa ttacgccagt tttgccagaa cggtgccgga gaatatgagc
     1381 aacaacgaga ttttccggca gtggaaggca ccagtcgttt gtccgccagc ctggcaacgg
     1441 gcgggttatc gcctcgccag tgtttgcatc gcttgttggc ggaacagccg caggcgctgg
     1501 acggtggggc cggtagtgtc tggcttagtg agctgatctg gcgcgagttc taccgtcatc
     1561 tgatgacgta ttacccctcg ttgtgtaaac attgtccgtt tattgcctgg acggatcgtg
     1621 tgcagtggca gagcaatccc gcacatttac aggcctggca ggaaggcaaa acgggatacc
     1681 cgattgtcga tgctgccatg cgtcagctta acagcactgg ctggatgcat aaccggctac
     1741 ggatgattac agccagtttt ctggtgaaag atttgttgat cgactggcgc gaaggcgagc
     1801 gatatttcat gtcgcagctg attgatggtg atttggcagc caataacggt ggctggcagt
     1861 gggccgcttc aaccggaacc gatgcagcgc cgtattttcg tattttcaac ccgacaaccc
     1921 agggcgagaa atttgaccgt gagggcgagt ttattcgtcg atggttaccg gagctgcgcg
     1981 atgtaccagg gaaagcggtg catgagccgt ggaagtgggc gcagaaagca ggtgtgatgc
     2041 tggattatcc gcaaccgata gttgatcaca aagaggcaag gctgcgaacg ctggcagcgt
     2101 acgaagaagc gaggaaagga gcctgataaa acgcagcgag cgttttatca ggtatggtgc
     2161 tggcctatta tgactccagc gccagcgcgc ggtttctgaa tttcagcgcc tgatacagcc
     2221 aaatcatcag taccagtcct acacacgcca gtgcgcccca ggtaatttga tcaaatactt
     2281 caatatatgc attgatggag tagttgatcg ccccggaagc atcaaacgaa gcctgcgatg
     2341 tctgatcggc aatcacgccc gcgagatagt tcgcaatcgc gccggaaagc agcatataaa
     2401 tgccggttaa tacgccggtc acaccgggga tttcaatgcg cgtaatttgc gacatggcaa
     2461 ccgggtcgat aaacagttcc gcaaagccca tcaccgccag gcctaatacc atcagtggca
     2521 gagaagagtg accatacatt gcggaccagc gggcgcttaa ggtcagaatg cagaacccgg
     2581 cgctcatcag gccgagacca agagcaaatt ttccccagat gcgcacggta cgattacctg
     2641 cgacgctttc tttaaccacc cacgccagga acacaccgca cagcataact gcgaaggcat
     2701 taatcgactg gaacatagca gtcggaacgg tataaccaaa catatcgcgg ttaacgaagc
     2761 ggtcgatata aaggctaatc gagctgccgc cctgttgtgc gaaggcccag aacaacatac
     2821 tgaagaaggt gagcgtcaca atcagcccca gctccttacg ctgtttctgg ttttctgctt
     2881 tgcgataaat ttttgccagt acacccaggc caataatagt cgcgacaatt aaggcgtata
     2941 ccgaccactc tttccagaac aatacggtaa tcagcgcagg cgttgccacc agcagaacca
     3001 gcagccatcc ccagttaggc aggagaaagt ttgtcgcacg cagtactttt ttattaacgc
     3061 cgcgggtatg tgtgaaatga cgattgccac ataagaaaat gaccagaccc gcgatcatac
     3121 ccaccgccgc caggccaaag cccatcgccc agctgtactc ttcctgggcg tagccacagg
     3181 caataggtgc gataatagac cccacgttac ccgctgcata catcagcgag aaaccgccat
     3241 cacgacgcgg atcggttggc tcatacagct cgccgagcag gcaactgaca ttggatttaa
     3301 acagaccata gccacaaaca attatcgcca gggacagata gaggaatgac ggatggatct
     3361 cactggcacc caacaccaca tgaccgatcg ccatcaataa cgcccccagc atcaccgcca
     3421 tgcgattgcc gagaacttta tccgccaaaa agccaccgag gattggcgtg acatacacca
     3481 gcgaacagta ggcgctaaat aactcgtagg cgtgagtatc gttgtatttt agttgattgg
     3541 tgagatagag aatcagcagg gcacgcatgc cgtaaaagct aaaatattcc cagatttgca
     3601 gcgcaacgac atagtaaata gcgcgcggct gtgatgcgtg tttattcata ataattccac
     3661 aattgacggc aacgtcacgt aatagttgcg cggcgtgctt gtgtttcctt aaagttaaaa
     3721 ctttgataca gatctgatta tttatgcaat atgctgtctg attgcataaa tatacattag
     3781 ctgaagcgtg atgatataaa caatttgcgt cttttggcgt cgggtttgtt tcttaatatg
     3841 tcaaaagatt ggctatcgac gaacaggcga ggtaacgcta tgttaacggt gtagctatca
     3901 gaatacggaa ggcgcgaaga tgaaaaacac cgaactggaa caactgatta acgaaaaact
     3961 gaacagcgcg gcgattagtg attacgcgcc gaatggtttg caggtggaag gcaaagagac
     4021 ggtgcaaaaa attgttaccg gtgtcaccgc cagccaggct ttgctcgatg aggcagtgcg
     4081 tctgggcgcg gatgcggtca ttgtgcatca tggctacttc tggaaaggag agtctccggt
     4141 cattcgcgga atgaagcgta accgtttaaa aacgttgctg gcgaatgata tcaacctgta
     4201 tggctggcat ttgccgcttg acgcacatcc tgagctgggc aataacgcac agctggcggc
     4261 gttactgggg atcacggtca tgggcgaaat tgagccgttg gtgccgtggg gagagctgac
     4321 catgcctgtg ccgggactgg aactagcctc ctggattgaa gcgcgtctgg gacgtaagcc
     4381 gttatggtgt ggcgataccg gacctgaggt ggtccagcgc gtcgcctggt gcacgggcgg
     4441 cgggcaaagt tttatcgata gcgccgcgcg ttttggcgtg gatgctttta ttactggcga
     4501 agtttctgaa cagaccattc attcagcccg cgagcaggga ttgcattttt atgctgcagg
     4561 tcaccatgcc actgaacgtg gtggtattcg cgcattgagc gagtggctga atgaaaatac
     4621 cgatcttgat gtgaccttta ttgatattcc taatcctgca taacgaataa tcagagggat
     4681 cgaaagtgca acgagcgcgt tgttatctga taggtgaaac ggcggtagtg ctggaactgg
     4741 aaccgccggt gacgctggct agccagaaac ggatctggcg actggcgcag cgtctggtgg
     4801 atatgccgaa tgtggttgaa gccattcccg gcatgaacaa tatcacggtg attttgcgta
     4861 atcctgagtc gctggcgctg gatgccatag agcttttgca acgctggtgg gaggagagcg
     4921 aggccctgga acctgagtct cgctttattg aaattccggt ggtttacggt ggtgcaggcg
     4981 gaccggattt ggcggtggtc gcggcgcatt gcgggttgag cgaaaaacag gttgttgaat
     5041 tgcactcctc cgtggaatac gtggtctggt ttttaggttt tcaaccgggc ttcccgtatc
     5101 tcgggagttt gccggaacaa ctacacacgc cacggcgcgc tgaaccgcgc ttgctcgttc
     5161 cggcaggttc tgtcgggatc ggcgggccgc agactggtgt ttatccgctg gcaacgccgg
     5221 gtggctggca gttgattggt catacctcac tcagcctgtt tgatccggcg cgtgacgaac
     5281 ccatcttatt acgtccggga gacagcgtgc gctttgtacc gcagaaggag ggagtatgct
     5341 gaagattatt cgtgcgggca tgtataccac tgtgcaggat ggcggtcgtc acggttttcg
     5401 ccagtcgggt atcagccact gcggcgcact ggatatgcct gcgttacgca ttgctaacct
     5461 gctggtgggt aatgacgcca atgcccccgc gctggagatc acgctcggtc agttaacggt
     5521 tgagttcgaa actgatgggt ggtttgctct gacgggtgcc ggttgcgaag cgcggctgga
     5581 tgataacgcc gtctggaccg gctggcgctt gccgatgaaa gcaggccagc gtttaacgct
     5641 taaacgcccg cagcacggca tgcgcagtta tctggcggtc gcgggtggta ttgatgttcc
     5701 gccggtaatg gggtcatgca gcaccgatct caacgtgggg attggcgggc tggaaggccg
     5761 tttactgaag gatggtgacc gactcccgat tggcaaatcg aagcgtgatt ttatggaagc
     5821 gcagggcgtt aaacagctgc tgtggggcaa ccgcattcgc gccttgccgg ggccggaata
     5881 tcatgagttc gatcgcgcct cgcaggatgc attctggcgt tcgccctggc agcttagccc
     5941 gcaaagtaac cgcatgggct atcgcttaca ggggcaaatt ttaaaacgca ccaccgatcg
     6001 cgaactgtta tctcacggtt tgttaccggg tgtggtgcag gtgccgcata acgggcagcc
     6061 gattgtgttg atgaacgacg cacagaccac cggtggttac ccgcgtattg cctgtatcat
     6121 tgaggctgat atgtaccatc tggcgcaaat tccgctcggt cagccgattc attttgtcca
     6181 gtgttcactg gaagaggcac tgaaagcgcg gcaagatcaa caacgttatt tcgaacaatt
     6241 agcgtggcgg ctacacaatg aaaattgacc tgaacgccga tctgggcgaa ggctgcgcca
     6301 gcgacgcaga gctattaacg ctggtttcct ctgccaatat tgcttgtgga tttcatgcag
     6361 gcgatgccca aaccatgcag gcttgcgtgc gtgaagcaat aaaaaatggt gtcgcgattg
     6421 gcgctcaccc gagttttccc gacagggaaa attttggtcg cagcgccatg cagctgccgc
     6481 cagaaaccgt ttacgcccag acgctgtatc aaattggcgc gctggcaacg attgcccgtg
     6541 cgcaaggcgg cgtaatgcgt catgtcaaac cgcacggcat gttgtacaac caggcggcga
     6601 aagaagcaca actggcagac gccatcgcca gagcggtata cgcttgcgat ccagcattgg
     6661 ttctcgttgg gctggcagga agcgagctga ttcgtgcagg caagcaatat ggcctgacaa
     6721 cgcgcgagga agtgtttgcc gatcgcggtt atcaggctga cggctcgctg gtgccgcgaa
     6781 gtcagccagg cgcgttgatt gaaaacgaag aacaggcgct ggcgcaaacg ctagagatgg
     6841 tgcaacacgg cagagtcaaa agtatcaccg gcgaatgggc aacggtcacg gcgcaaacag
     6901 tctgcctgca tggcgacggc gagcacgcac tggcgttcgc ccgccgacta cgctctacat
     6961 ttgccgaaaa ggagattgtt gtcgcagcat aaccccgatt aataaagaat gaaaaaagga
     7021 tatcaccatg cctgaaggcc cggagatccg ccgtgcagcg gataacctgg aggcggcgat
     7081 caaaggcaaa ccactaactg atgtctggtt tgccttcccg cagttaaaaa cttatcaatc
     7141 acaacttatc ggtcaacacg ttacccatgt gggaacgcgt ggtaaggcgt tgttaactca
     7201 tttttccaac gacttaacgc tctacagcca taatcagctt tacggcgtct ggcgcgtggt
     7261 tgataccggc gaagagccgc agaccacgcg agtattgcgg gtaaaactgc aaacggctga
     7321 caaaaccatt ctgctttata gcgcctcgga tattgagatg ttgaggccgg aacaactgac
     7381 cacgcatccg tttttacaac gcgttggtcc cgatgtgctg gatccgaatc tgacgccgga
     7441 ggtggtaaag gaacggttat tgtcaccgcg ctttcgtaac cgtcagtttg caggattact
     7501 gctcgatcag gcgtttctgg ctgggcttgg caattatttg cgggtggaga tcctctggca
     7561 ggttgggttg actggaaatc ataaagcgaa agatctcaat gcggcgcaac tggatgcact
     7621 cgcacacgcg ttactggaga ttcctcggtt ttcttacgct acgcgggggc aggtggatga
     7681 gaataagcat catggggcgc tgtttcgctt taaggttttt catcgtgatg gcgaactgtg
     7741 cgaacgctgt ggcggcatca ttgagaaaac cacgttgtcc tcgcgcccgt tttactggtg
     7801 tccaggctgc cagcactagg ccgaccgctt cggcgcatag gttgaaataa accgcgcaat
     7861 ggctggcccc gtcagcaaaa tactgaacag gcgcagggtt tgcatcgcca taataagcgc
     7921 catatcggca ttgctccctg cggcgatgac cgccacggta tcaagcccgt cgggactggt
     7981 ggcgaggtag gcagtcataa aatcaatatg cataaaccgg gtcagtcccc acgccatacc
     8041 tgcacaaata gccagcagag caaaaatcga cagcaggatt tgcggtagtg ggcgtagtgc
     8101 ccgcagtaag atttgtttat cgaaaccaag accaatccgc cagccaattg ccatatacgc
     8161 catcgccagc agccattccg gtagttcgat ggtgatgagc tgaccagact ggagcactgc
     8221 gcccgccagc atcgggatga gcatcgtgcc ggaaggaaga cgcagcatgc atcccgccgt
     8281 accggcaacg actgcgagca aaatcgtaag caggagatta atgctcactg gcgggaacca
     8341 gacgatttgc tggttaaccg cttcagcgtt atcacccagc atcatgcagg tgaccagaac
     8401 agcggccccc gcgacgaaca gcactcgcag atattgcata aacgccacca ggcgaatatc
     8461 tgcgccgtaa tcttgcgcca tagcgaccat tgccgctgcg ccgcctggag aagaacccca
     8521 ggcaccggta tttccaggca gtgagctata gcgcaccaat aaccaaccca cgatggcgct
     8581 ggaaagcagc gtcaccagta aaatagcaag cacgatcggc cagttggcgg ccagcgtggt
     8641 gagaatcgaa ccagtgaggt tttgcgcaat catgcagcca agaatggcct gcgcggcgag
     8701 aaaagcggag cggggaagtt gcagagtaat tccgcgcata ctgaagatga tcccggcgat
     8761 catcggccca agtaatagcg ccgccgggag atgtaccgcg aggaaaccaa tagaaaggag
     8821 aagtgatagc acacataaca ttccccactg caaaactggc atccttcgct ccctcattat
     8881 tttctctttg gtgtatcgat acagcataaa gaaagccggg ccaggaaaaa ataacagtaa
     8941 tcaaggaaaa gaggaaaatc a
//
LOCUS       DAJTYD010000158         8829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTYD010000158 DAJTYD010000000
VERSION     DAJTYD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.158"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..189
                     /locus_tag="OO585_003269"
     CDS             <1..189
                     /locus_tag="OO585_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6630756.1"
                     /translation="ISSLVNSLKGVSGRLLRRDRPDIAVRYYYKGVLWSPGYFANSCG
                     GAPISVIRQYIEQQQTPG"
     gene            complement(320..1643)
                     /locus_tag="OO585_003270"
                     /pseudo
     CDS             complement(320..1643)
                     /locus_tag="OO585_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1643..3259)
                     /locus_tag="OO585_003271"
     CDS             complement(1643..3259)
                     /locus_tag="OO585_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6630757.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3555..4271
                     /locus_tag="OO585_003272"
     CDS             3555..4271
                     /locus_tag="OO585_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6630758.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4268..5929)
                     /locus_tag="OO585_003273"
     CDS             complement(4268..5929)
                     /locus_tag="OO585_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6630759.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6125..6553)
                     /gene="ibpB"
                     /locus_tag="OO585_003274"
     CDS             complement(6125..6553)
                     /gene="ibpB"
                     /locus_tag="OO585_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6630760.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6665..7078)
                     /gene="ibpA"
                     /locus_tag="OO585_003275"
     CDS             complement(6665..7078)
                     /gene="ibpA"
                     /locus_tag="OO585_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6630761.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7072..7144)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7384..7716
                     /locus_tag="OO585_003276"
     CDS             7384..7716
                     /locus_tag="OO585_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6630762.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7718..8728)
                     /locus_tag="OO585_003277"
                     /pseudo
     CDS             complement(7718..8728)
                     /locus_tag="OO585_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
BASE COUNT         2241 a         2338 c         2216 g         2034 t
ORIGIN      
        1 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
       61 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
      121 aatagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
      181 cctggttagg tggaaaaccg cgccttatat ccccgccctg aaggacgggg ttttacggcg
      241 caccggataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
      301 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
      361 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgat aacgtaatcg
      421 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
      481 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
      541 ctggtccgtt gtgcccgacc agacacggga tctcgaccat cgcttcatca tcaaagttat
      601 ggattgcccc gttgttaggc acaatcagca acatccgctc ctgagtgttg aaggcaattg
      661 ccgccgccag atcgacgatg tatgacgcat gttcgtcaat ctccagcttg gccgccgctg
      721 aatttcctgc cgctgtaatg gcgcggcaag catcgaaaac ctgtttctcg cgatgttcca
      781 ttcacctcat tcgcgcgggt atgttcaggg ttggaatgct gaacaacata atccgggaag
      841 agataatatt tcagataggt gttcggcaat gtatccgggt ccagtgcctg gacatcccgc
      901 gcttttgcat aggtgtcgtt ccagctcgct tcagtatgtg tatcttgctg cggtggaaca
      961 taaccgtgct tagagacata ttgccgcagc tggggcatca ggtcgttgcc ctgcaaatct
     1021 tcaattgatg tccaccagcc aaagtggttc aggccgtagt agcgcacgcg catctgtttg
     1081 cgatcttgca gcccaacaat ttgcgccatc cggctttcaa taccgattgg catgtcacag
     1141 atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac aattgctgcc
     1201 gggttggagt agttgagcat ccaggcattt ggtgaatatt tttccatata atccaccagt
     1261 tccaggacgc cgccaatgga acgcatgccg tacgctattc cgccgggtcc gcaagtttcc
     1321 tgaccaacaa cgccgtggcg cagcgggatt ttttcatcca gttcgcgcat cgggtatttg
     1381 ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga atgccacttc aggatcggtg
     1441 gtgtaactaa acgcaatgtc cggcgctttc tctttaagga tgactttaca ggcttcggca
     1501 atcacttcct gccgcgcacc atcgttatca taaaatttca gtgcacgaag cgggaaacgg
     1561 tcctgattcg ccaggagcat caacacgatg cctggcgtaa aggtgcttcc accgcctgcg
     1621 acaaccactg agaatttggt cattatactg cctccgtaat ggcaacattt tctgctgatt
     1681 gatgagaatt aattaagcta tcgagctgtt cacgcagctg ggatacatgc agaccaatga
     1741 tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt
     1801 cgtcatccag cgtttgtgac atgtcatgca gtgcaatacg taaacgcgtc gcgcaattgt
     1861 taatgctgga gatattgccg accccgccca gggcttgcag gataccggca gcctgatcca
     1921 gctctttttt tggctctgca gcggtggttt ggcctcgcga ggctttgtat tcggcttttg
     1981 agtagagttt cacttccgca tcttcacgtc ccggcgtgca catgttgaat tgcagaatca
     2041 gtgtgcggaa aaccacgaag tacagcaggg taaagcacaa cccaatggcg atttgggtca
     2101 gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa
     2161 tcagacctcc gcccatgttg cccaccacac caaagagata catcacggtc gacattgagg
     2221 ccgccagcac ggcgtgtacc gcaaacagca acggtgaaat gaacaggaag gtaaattcca
     2281 gcggttcggt aattcccacc agcatggcgg ttaaggttgc gggaatcagc aagcccgcca
     2341 cttttacccg attttccggt gcggcagtga agtacatcgc taaagaaatg cccacggcac
     2401 caaagatttt tgagttaccg tgcagggcaa aaccgccttc cgggaacaac gatttcagcg
     2461 gctcggcgct cagactgaac tcttgcagat gctgcgccca gtacatctga atgccgcctt
     2521 caacagctgc cggaccaaag ataaactgtc cgtagatgaa gtggtgtaaa ccggttggga
     2581 tcagaatacg ttcgaggaag gtgtaaaccc accccccaag cgcacccgcc gaacgcagga
     2641 acgcttgcag agattcaatc cccatttgta cttttggcca accgagcaac gtcagccagg
     2701 cacaggggat catcaccagg aaggcgataa tcaccacata agacgtcccc tggaaaatgc
     2761 cgagaaaaac cggcagtttt ttatcgaaca gacggttatg cagcgccgtc acaatgccgg
     2821 aaatgataat tgcgccgata atgctggtat cgagggtttt aatcccggcc atcattgtca
     2881 gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt ccccagatca
     2941 ttcccatcgc gttgatgaaa tagttccagg tcaggaaact caccatcacc gccagacaag
     3001 cacgcccctg cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca
     3061 tattacggaa taccgtccaa ccgccctctt caataatgtg tacgatttgc gcgaataaac
     3121 tgttcggatc ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag atggcaagac
     3181 ccaccacaat cccggcgaag ggaaacagca gcactggcgt gaacatcgcg ccgccaaagc
     3241 gttgaatttg actgagcatt tccacattct cttttctcaa ttctgtaagt aggaagcctg
     3301 attggttatg cggcaggcct ggagcgagga taggaagatg cgatcggcag aacatgacga
     3361 cccaatggga ttcgtgatcg ctttcatgct tttcgttggg cggtagagat ctacgttttc
     3421 gcgaaaaagt agacatctgt gagcactggt acacagcatc gaacacatct ttaaaaaaaa
     3481 gatgtttttt caatcgatta agcagaactt gtgggcgcat tacccgggct tgcaggcaaa
     3541 aaagagatct agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaatt
     3601 ccgcggattt cacgctaaac agccttcttc ccggtgaaaa aaagttggcg gaagaatttg
     3661 cagtatcccg gatgaccatc cgtaaagcga ttgacctgct ggtagcgtgg gggctggtgg
     3721 tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtgctgcat caaaccgcca
     3781 gcctgaccgg actggtggag gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc
     3841 tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca
     3901 acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctgatgctgg
     3961 aagacagcta tatgccggtt aaactgttcc gtaatctttc gctgcaacat ctggaagggt
     4021 cgaagtttga atatattgaa caagagtgtg ggattttgat tggcggtaat tatgaaagcc
     4081 tgacgccggt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc
     4141 cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg aattattcag
     4201 tgatgttcag aaatgccagc gaataccagg tggagtacca tttacggcga ctccacccgg
     4261 aaaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg ataattcgca
     4321 gaaacatcac taacggatag acagtggcgt aagagagcgc cggcgcacca ctggttggat
     4381 gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc
     4441 acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg
     4501 gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca ttgaccaggg
     4561 tattcacaaa atccccacca gatttcagac caacgaccga gaggaacagc acgatcccca
     4621 gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg ccgatactgc
     4681 cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc agtttcaacg
     4741 cggccgggaa cccaggcaca aagacgggaa tagaacctaa caatacgcca agcccgatgc
     4801 caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat
     4861 tggcaacggc atcaattgcg gacggacgcc ccaccagatt gaggatatcg ccgaactgca
     4921 ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa
     4981 cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg
     5041 tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt ggacagcgaa gtatcgacct
     5101 cctgaccaat caccagttgc gcattatgta aatccgctgg ctgccccacc agatgcagca
     5161 aatcgcccag ttggataatg gtatctggcg aaggaacttt tagggtttct tcgcgtttca
     5221 gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaata gctaaatcat
     5281 gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg ccattggtgc
     5341 gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata acccgcaaca
     5401 tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg taactcatcc
     5461 ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caagatctgc tgccctgccc
     5521 ccagcgctgg cgtattggta accgcaccgg agaaaatccc cagcactacc ggcagtggaa
     5581 tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag
     5641 caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctactt
     5701 gaatcccgat ggtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat
     5761 cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca
     5821 gcacgccgcc aatacctaat cctatgccgc gaaatttgac gttgccgata aacaaaccga
     5881 cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct
     5941 cttgagataa cattttaatt gctacggatt atggctgaat cggtgaagca gatttgcctt
     6001 atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc ttaaacagtc gaaatttaac
     6061 gcattgttga agaagaaaga caaaaaaagc cccaccagaa tggcggggca aagagaatag
     6121 ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt
     6181 caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt gcgccagaga
     6241 cttccatatt ttcagccagc gtaaagctca ggctaaatgg ctgattcata agcccttgat
     6301 gcagccattt tttctcttct tttggctgct ccggcgtgcc ttttacgctc aggcgcgtac
     6361 cttccagttg aatctctaaa tcttcctgac ggaaacctgc cagcgcaagg gtaatgcggt
     6421 agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctggctt tcaccggcgt
     6481 tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt ggggataaat
     6541 cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc aaatccgaag
     6601 attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgtcag gcggccttag
     6661 ggaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg cgttcgagat
     6721 cgatatacag caaaccattt accaggttag ccccacgaac atgaatgttc tcagctaact
     6781 ggaatttgcg ttcaaagttg cgttcagcga tgccctggta cagataggtg cgctcttttt
     6841 gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta atttccagtt
     6901 cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg tctaccagtt
     6961 caacgttata cggagggtag ccgccattac tctggctctg gttgttttct aagtggttaa
     7021 acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag ttacgcataa
     7081 tcaatagctc ctgaaatcag cgagaatgta ataccttcca caatggacag gtcaggtagc
     7141 cagaacaccc atcaggcgcg tcctcatcgg ctacgatgta aaaatgggtc tggaaatgaa
     7201 cttttcaagc cttatcagcg gacttttttt tgcagtttat ggtctattgc atagactgag
     7261 ggggcagcac gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat gatgatgtta
     7321 ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac agatgaattt
     7381 attatgataa gaaatgtgtt gttagcgttc atgatatgca gcggaatgac attactcgga
     7441 ggatgctcca gcgtgatgtc ccataccgga gggaaagaag gaacgtatcc cggcacccgc
     7501 gccagcgcaa ccatgattgg cgacgatgaa accaactggg gcactaagtc gctggcgatt
     7561 ctcgatatgc cgtttaccgc tgtgatggac acgcttttac tgccgtggga tgtgttccgc
     7621 aaagacagct cggtgcgctc acacgttgaa aaaagcgaag ccaacgcgca agccaccaat
     7681 gcggttattc caccggccag aatgcctgat aactgattta gcgatcagtt tcggtgatcc
     7741 acagctggcc gccttccatc cacgccagcc attgaccatc cggcgagaag accacggcgt
     7801 ccgcagaagg tggattggcg tggttttcgg ttaaatactc aacctcgcca ctttgcgcat
     7861 gggcacaagc aattcgatta tccagcacaa agcccaacca ttctcctgac ggatgccagt
     7921 taaatgcaga ctgaatatcc gttttgttat gggttaactg gcgcggctcg ccgccctgtg
     7981 gcgagataag ccacagttgc acaatgccgt tatcatcacg cattaaaaac gcgatttgcg
     8041 taccctgcgg attacagcgc acccagtggc gcgggacgtt gactaacccc ggataagccc
     8101 gatggtgggt aaaggttaaa cgtcgctgca cgacgccgcg cggtggcgcg ggcagggtgg
     8161 tttccgttcc acttaacggc gcatcacctg ccgctttcca gccagcttca tcttgcggta
     8221 actcaacgat aaacagctcc ggcactttct cgccctttgg cgaaagtgtg tcgccaataa
     8281 acgccagcgc gtgatttcct acccatcctt cttcataagc acgattgatt tcatcgctgc
     8341 caggctgcgg cgtgggcgtg gttttactca ccagcacgca ccagtggcta ccgctgtatt
     8401 cacgcggatg ctgcttttgt acgttgaccg ggccaaacgg cgcagcaaca ccgacgtttc
     8461 gcaaatccag cgccggatcg agttcatgca ttacatggtc gttataggta aagctcaccc
     8521 tttcaccgtt cgggctaaag acatgcacat ggctgccgcc gcgcagcgct cctggcgtat
     8581 acggcgcggt aatatccatt gcatcgagat tgctcacctt gccgccttca gcaatgactc
     8641 cgcgtcgatg atggaaatcg taataccatg tttcatcagg attttccggg ccatggatga
     8701 aaacatattt ctctgacttc ggatgaacca cggcagcctg acggctgcaa attgaaaacc
     8761 gcccctgttc gtcctgttgt tgccgcacgc tccgcagaat atgtatgctc tgaggacctg
     8821 ttttattaa
//
LOCUS       DAJTYD010000159         8690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTYD010000159 DAJTYD010000000
VERSION     DAJTYD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.159"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..409
                     /locus_tag="OO585_003278"
     CDS             <1..409
                     /locus_tag="OO585_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630763.1"
                     /translation="IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEM
                     HGDAALVEAIRNDRMPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMG
                     VAKPVHVLTPIASVRRIVNMVALAVVEAQTQPL"
     gene            701..1036
                     /gene="eutS"
                     /locus_tag="OO585_003279"
     CDS             701..1036
                     /gene="eutS"
                     /locus_tag="OO585_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6630764.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            1049..1528
                     /gene="eutP"
                     /locus_tag="OO585_003280"
     CDS             1049..1528
                     /gene="eutP"
                     /locus_tag="OO585_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6630765.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            1503..2204
                     /gene="eutQ"
                     /locus_tag="OO585_003281"
     CDS             1503..2204
                     /gene="eutQ"
                     /locus_tag="OO585_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6630766.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            2201..3010
                     /gene="eutT"
                     /locus_tag="OO585_003282"
     CDS             2201..3010
                     /gene="eutT"
                     /locus_tag="OO585_003282"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6630767.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            3000..4016
                     /gene="pta"
                     /locus_tag="OO585_003283"
     CDS             3000..4016
                     /gene="pta"
                     /locus_tag="OO585_003283"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6630768.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            4055..4348
                     /gene="eutM"
                     /locus_tag="OO585_003284"
     CDS             4055..4348
                     /gene="eutM"
                     /locus_tag="OO585_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6630769.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            4455..4742
                     /gene="eutN"
                     /locus_tag="OO585_003285"
     CDS             4455..4742
                     /gene="eutN"
                     /locus_tag="OO585_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6630770.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4754..6157
                     /locus_tag="OO585_003286"
     CDS             4754..6157
                     /locus_tag="OO585_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6630771.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            6168..7004
                     /gene="eutJ"
                     /locus_tag="OO585_003287"
     CDS             6168..7004
                     /gene="eutJ"
                     /locus_tag="OO585_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6630772.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6994..8616
                     /gene="eutB"
                     /locus_tag="OO585_003288"
     CDS             6994..8616
                     /gene="eutB"
                     /locus_tag="OO585_003288"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HCR6630773.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
                     RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSN
                     ADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAV
                     IGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQS
                     ICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQV
                     TMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDC
                     CYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLNL
                     RPSPEFERWLESMGIMANGRLTKRAGDPSLFF"
BASE COUNT         1985 a         2276 c         2583 g         1846 t
ORIGIN      
        1 tattgagccg cgcgttgctc tgttgtcgca ctccaacttt ggttcttctg actgcccgtc
       61 gtcgagcaaa atgcgtcagg cgctggaact ggtcagggat cgtgcgccag atctgatgat
      121 tgatggtgaa atgcacggcg atgcagcgct ggtggaagcg attcgcaacg accgtatgcc
      181 ggacagccct ttgaaaggtt ctgccaatat tctggtgatg ccgaacatgg aagctgcccg
      241 cattagttac aacttactgc gcgtttccag ctcggaaggt gtgactgtcg gtccggtgct
      301 gatgggcgtg gcgaaaccgg ttcacgtgtt aacgccgatc gcatcggtgc gtcgtatcgt
      361 caacatggtg gcgctggccg tggttgaagc gcaaacccaa ccgctgtaat tcttttttaa
      421 ctctcacgct taccctgaat attcagggta agcggtttgg ctgcaatata ttagtaaagc
      481 ttattactga gtttgcgaat aataaaaaag cagtctatat aatatctcga tattatttta
      541 tttatattca tgcgttgcat atgaaagttt atgcaccaca gcgaatatct ctccattctt
      601 agtgatctac ctcacctttt aaacgcgctt gccgaatttt gttatttact ctgacgaaaa
      661 attgtcacga tacacgaaag tttttcacag gcggcgactc atggataaag aacgcatcat
      721 tcaggaattt gtgcccggca aacaggtcac actggcgcat ctcatcgcgc atcccggtga
      781 ggaactggcg aagaaaatcg gcgttccgga tgcgggcgca attggcatta tgacgctaac
      841 tcccggcgaa acggcgatga tcgccggtga tttagcgctg aaagccgctg atgtgcatat
      901 cggttttctc gaccgcttca gcggtgcatt ggtgatttat ggctcggtgg gcgcagtaga
      961 agaagcgtta tcacaaaccg tcagcggttt aggtcgctta ttaaattata cgctttgtga
     1021 aatgaccaaa agttaatacg aggcattcat gaaacgaatt gcgtttgtgg gtagtgtcgg
     1081 ggcaggaaaa acaacgttat ttaatgcgtt gcaggggaat tataccctcg ccagaaaaac
     1141 acaggccgtg gaatttaatg ataagggcga tattgatacg ccgggtgaat attttagcca
     1201 tccccgctgg tatcacgcct taattaccac gctacaggat gtagacatgc tgatctatgt
     1261 ccatggcgca aatgacccgg aaagtcggtt gcctgccggg ttgctggata ttggcgtcag
     1321 caaacggcaa atcgccgtca tcagtaaaac ggacatgcca gatgctgacg tcgccgcaac
     1381 gcgaaagtta ttgcttgaga cgggtttcga agagccgatt ttcgagctaa acagccacga
     1441 cccgcaaagc gtgcagcaac tggtggatta tctggcatct ctcaccaaac aggaggaagc
     1501 aggtgaaaaa actcatcaca gcgaatgata ttcgtgaagc acacgcgcgc ggcgaacagg
     1561 cgatgtccgt ggttctgcgc gccagcatta ttaccccgga agcccgtgaa gtggcggatc
     1621 tactgggttt taccatcacc gaatgtgatg aatcgatccc ggtaacggcg tccgtgcccg
     1681 ccagcgtgcc agccgacaaa accgaaagcc agcgcatccg cgaaaccatc attgcccagc
     1741 tgccggaagg ccagtttacc gaaagcctgg tcgcacagct gatggaaaaa gtgatgaagg
     1801 aaaaacagtc gctggagcag ggcgcaatgc agccgagctt caaatcggtg accggcaaag
     1861 gcggcatcaa agtgattgat ggcagcagcg tcaagtttgg tcgtttcgac ggcgcagagc
     1921 cgcattgcgt gggcttgact gatcttgtga ccggagacga cggcagcagc atggccgccg
     1981 gttttatgca gtgggagaac gcattcttcc cgtggaccct gaactacgac gaaatcgaca
     2041 tggtgctgga aggggtgctg catgttcgcc acgaaggcca aaccatgatc gccaaagcag
     2101 gcgacgtgat gtttatcccg aaaggctcca gcatcgaatt tggtatgaca tccagcgtga
     2161 aattcctgta tgtcgcctgg ccggctaact ggcaatccct atgaaagatt tcatcaccga
     2221 agcatggcta agagcgaacc atacgctcag cgaaggcgca gagatccatc tccctgcgga
     2281 cagtcgcctg acgccctctg cccgggagtt gctggaaagc cgccatctgc gcatcaagtt
     2341 tattgacgag cagggccgcc tgtttgttga cgatgaacag cagaagccgc agcccgttca
     2401 tgggctgacc agtagcgatg aacatccgca ggcgtgctgc gaactgtgcc gccagccggt
     2461 ggcaaaaaag ccggatacgc tgactcacct gtcggcggaa aaaatggtcg ccaaaagcga
     2521 tccgcgtctg ggttttcgcg cggttctcga cagcaccatt gcgctggcag tgtggctgca
     2581 aattgaactg gcggaaccgt ggcagccgtg gctggcggat atccgttcgc gtcttggcaa
     2641 cattatgcgc gccgatgcgc tgggggaacc gctgggctgc caggcgattg tcgggctttc
     2701 tgacgaagat ctgcatcggc tttctcatca gccgctgcgc tatctcgacc acgatcatct
     2761 ggtgctggaa gccagccacg ggcgcgatgc tgcgttgctc aatctgctac gcaccaaagt
     2821 gcgggaaacc gaaaccgtag cggcgcaggt ctttatcacc cgcagctttg aagtattacg
     2881 cccggacatt ttgcaggcgc tgaaccgcct ttccagcacg gtctacgtga tgatgatcct
     2941 gagcgtaacg aagcagccgc taacggtgaa gcagatccaa caacgactgg gggaacgcaa
     3001 tgattattga acgttgtcgt gaactggcgt tgcgagcgcc cgccagagtg gtttttccgg
     3061 atgcgttaga tcaacgtgtg ctgaaagccg cgcaatattt acatcaacaa ggtctggcaa
     3121 cgcccattct ggtcgccaat ccgtttgaac ttcgtcagtt tgcgctcagt cacggcgtgg
     3181 cgatggacgg gctacaggtg attgatccgc atggcaacct cgcaatgcgg gaagaatttg
     3241 ctcatcgctg gctggcccgc gcgggcgaaa aaacgccgcc ggatgcgctg gaaaaactta
     3301 ccgaccctct gatgttcgcc gcagcaatgg tcagcgccgg taaagcggat gtctgtatcg
     3361 caggcaacct ctcttccacg gcaaatgtgc tgcgtgccgg attacgcatt atcggcttgc
     3421 agccaggctg taaaacgctc tctttcattt tcctgatgtt gccacagtac agcggcccgg
     3481 cgttgggctt tgccgattgc agcgtggtgc cacagccgac ggcggcgcag ctggcggata
     3541 tcgcgcttgc cagtgccgaa acctggcgcg ccatcaccgg agaagagccg cgcgtggcga
     3601 tgctgtcgtt ttccagcaat ggtagcgccc gtcacccctg tgttgctaac gtccagcagg
     3661 cgacagaaat cgtccgtgag cgcgcaccaa agctggtggt ggatggcgag ttacagtttg
     3721 acgccgcctt cgtgccggaa gtggcggcgc aaaaagcgcc tgccagcccg ctacagggca
     3781 aggccaatgt gatggttttt ccgtcgctgg aagccggaaa tattggttac aacatcgcac
     3841 aacgactcgg cggatatcgt gccgtcgggc cactgataca aggacttgcc gtgccgatgc
     3901 acgatctctc tcgtggttgt agcgtgcagg aaattatcga gctggcgctg gtggcagctg
     3961 tgccgcgtca gacagaagtg aaccgcgaaa gcagtttaca aacactggtt gaatgaacgg
     4021 tcccgttctg gaccctgata aagaggaaag cacaatggaa gcattaggaa tgatcgaaac
     4081 ccggggcctg gttgcactga ttgaggcttc tgacgcgatg gtgaaagctg cgcgcgtcaa
     4141 gctggttggt gttaagcaga ttggcggcgg cctgtgtact gccatggttc gtggtgatgt
     4201 tgcagcctgt aaagccgcga ccgatgcagg tgccgcagcg gcacagcgta tcggcgagct
     4261 ggtatctgta cacgttatcc cgcgtccgca cggcgacctg gaagaagtct tcccgatcgg
     4321 cctgaaaggc gatagcagca acctgtaatc accctgtgtg acgtcgattc gggcgttacg
     4381 ctctctcctt tttcatttta ccttctgcgg agggtagggg gagcttttac ctgaaatacg
     4441 ccacggaggc gggtatgaaa ctggcagtcg tcactggaca aattgtttgt accgtacgcc
     4501 atcacggact ggcgcatgac aaattgctga tggtggaaat gattgatcca caaggtaatc
     4561 ctgacgggca atgcgccgtc gccatcgaca atattggcgc gggaaccggg gagtgggtgt
     4621 tactggtgag tggcagttcc gcccgccagg cgcataaaag cgaaacgtca ccggtcgatc
     4681 tgtgcgtgat tggcattgtc gatgaggtgg tgtctggcgg tcaggtaatt ttccacaaat
     4741 aaggcagaac atcatgaatc aacaggatat tgaacaggtg gtgaaagcgg tactgctgaa
     4801 aatgcaaagc agtgacacgc cgcccgccgc cgttcatgag atgggcgttt tcgcgtccct
     4861 ggatgacgcc gttgcggcag ccaaagtcgc ccagcaaggg ttaaaaagcg tggcaatgcg
     4921 ccagttagcc attgctgcca ttcgtgaggc aggcgaaaaa cacgccagag atttagcgga
     4981 acttgccgtc agtgaaaccg gcatgggccg cgttgaagat aaatttgcca aaaacgtcgc
     5041 tcaggcgcgc ggcacgccag gcgttgagtg tctctctccg caggtgctga ccggcgacaa
     5101 cggcctgact ttaattgaaa acgcgccctg gggcgtggtg gcttcggtaa ctccgtccac
     5161 taacccggcg gcaaccgtaa ttaataacgc catcagcctg attgccgcgg gcaacagcgt
     5221 catttttgcc ccgcatccgg cggcgaaaaa agtctcccag cgggcgatca cgctgctcaa
     5281 ccaggcgatt gttgccgcag gcgggccgga aaacttactg gttactgtgg caaatccgga
     5341 tatcgaaacc gcgcagcgtt tgttcaagtt tccgggcatc ggccttctgg tggtgaccgg
     5401 cggcgaagcg gtagtagaag cggcgcgtaa acacaccaat aaacgtctga ttgccgcagg
     5461 cgctggcaac ccgccggtag tggtggatga aaccgccgac ctcgcccgtg ccgctcagtc
     5521 catcgtcaaa ggcgcttctt tcgataacaa catcatttgt gccgacgaaa aggtactgat
     5581 tgttgttgat agcgtagccg atgaactgat gcgtctgatg gaaggccagc acgcggtgaa
     5641 actgaccgca gaacaggcgc agcagctgca accggtgttg ctgaaaaata tcgacgagcg
     5701 cggaaaaggc accgtcagcc gtgactgggt tggtcgcgat gcggcgaaaa tcgcggcggc
     5761 aatcggcctg aacgtgccgc aagaaacgcg tttgctgttt gtggaaacca ctgcagaaca
     5821 tccgtttgcc gtgaccgaac tgatgatgcc ggtgctgccc gtcgtgcgcg tcgccaacgt
     5881 ggcggatgcc attgcgctgg cggtgaaact ggaaggcggt tgccaccaca cggcggcaat
     5941 gcactcgcgc aacatcgaaa acatgaacca gatggcgaat gctattgata ccagcatttt
     6001 cgttaagaac ggaccgtgca ttgccgggct ggggctgggc ggggaaggct ggaccaccat
     6061 gaccatcacc acgccaaccg gtgaaggggt aaccagcgcg cgtacgtttg tccgtctgcg
     6121 tcgctgtgta ttagtcgatg cgtttcgcat tgtttaagga gtaacagatg gcgcacgacg
     6181 aacaatggct caccccacgt ctgcaaacgg cggcaacgct gtgtaaccag acgcccgccg
     6241 cgacggaatc tccgctgtgg ctgggggtgg atctgggcac ctgcgatgtg gtgtcgatgg
     6301 ttgtcgaccg cgacggtcag ccggtggcgg tatgcctcga ctgggccgat gtggtccgcg
     6361 acggcatcgt ctgggatttc ttcggcgctg tcaccattgt tcgtcgccat ctcgacacgc
     6421 tcgagcagca attcggtcgc cgttttagcc atgcggcgac ttcatttcct ccaggtacgg
     6481 acccgcgtat ttccatcaac gtgctggagt ctgctggact ggaagtgagc catgtgctgg
     6541 atgagccaac cgcagttgcg gagctgctgc aactggacaa cgccggtgta gtggatatcg
     6601 gcggcggcac taccggcatt gccatcgtga aaaagggcaa ggtgacgtac tcggcggatg
     6661 aagcaaccgg cggacatcac atctctctga ctctcgccgg aaatcgccgt atttcgctgg
     6721 aagaggcgga gcagtacaag cgcggtcacg gtgaagagat ttggcctacg gtgaaaccgg
     6781 tgtacgaaaa aatggcggac attgtcgccc ggcatattga agggcagggc attactgatt
     6841 tatggctggc gggcggctct tgtatgcaac cgggcgtggc ggaactgttt cgcaaacaat
     6901 tcccggcgtt acaggtgcat ttaccgcagc acagtttgtt tatgaccccg ctggcgatcg
     6961 ccagtagtgg gcgagagaaa gcggaggggc tatatgcaaa atgaattgca gaccgcgctc
     7021 tttcaggcgt tcgataccct gaatctgcaa cgggtaaaaa catttagcgt tccaccggtg
     7081 acgctttgcg gtccgggctc ggtgagcagt tgcggacagc aagcgcaaac gcgtgggctg
     7141 aaacatctgt tcgtgatggc agacagcttt ttgcatcagg cagggatgac cgccgggctg
     7201 acgcgcagcc tggctgttaa aggcatcgcc atgacgctct ggccatgtcc ggtgggcgaa
     7261 ccgtgcatca ccgacgtgtg tgcagccgtg gcgcagttgc gtgagtcagg ctgtgatggg
     7321 gtgatcgcat ttggcggcgg ctcggtgctg gatgcggcga aagccgtggc gttgctggtg
     7381 acgaacccgg atagcacgct ggcagagatg tcagaaacca gcgttctgca accgcgcttg
     7441 ccgctgattg cctatgaaga tgactgcgtg acgcggctga ttcaggacga tgttaacgaa
     7501 acggcctaca accagattaa aaactggagc atcagcgaac tgcgtgagta tgtgctgagc
     7561 gatgaaacta gcgtggacga cattgccttt acccgcaaag ggctgacctc ggaagtggtc
     7621 gcggcggtag cgaagatttg ctccaacgcg gacctgatct acggcgcgaa gaaaatgccg
     7681 gtaatcaaaa aggccaatac caccatcggt attccgggca ccttcagcgc ccgtttgcag
     7741 ccaaatgaca ctcgtgacga cgtgcaaagt atcgccgcgc aaatctacga agggctttcc
     7801 ttcggggtgg gcgatgcggt gatcggcgtc aacccggtaa ctgacgacgt ggaaaactta
     7861 agccgcgtgc tggataccat ttatggcgtg atcgacaaat tcaacatccc aactcagggc
     7921 tgcgtactgg cgcacgtcac cacccagatc gaagcgattc gtcgcggcgc gcctggcgga
     7981 ctgattttcc agagtatctg tggcagcgaa aaagggctga aagagtttgg cgtggaactg
     8041 gcgatgctcg acgaagcgcg cgcagtgggt gcagagttca accgtatcgc cggggaaaac
     8101 tgcctctact tcgaaaccgg acaaggctct gcgctatccg ctggcgctaa cttcggcgct
     8161 gaccaggtga cgatggaagc acgtaactat gggctggcgc gtcattacga tccgtttatc
     8221 gtcaacaccg tggttggctt tattgggccg gagtatctct acaacgaccg ccagattatc
     8281 cgtgctggct tagaagatca ctttatgggc aagctgagcg gcatctctat gggctgtgac
     8341 tgctgctaca ccaaccacgc tgacgctgac cagaacctca acgaaaacct gatgatcctg
     8401 ctcgccaccg caggctgcaa ctacatcatg gggatgccgc tgggtgatga catcatgctc
     8461 aactaccaga ccaccgcatt ccacgatacc gccactgtgc gtcagttact caacctgcgc
     8521 ccgtcaccag agtttgaacg ctggctggaa agcatgggca ttatggcaaa cggtcgcctg
     8581 accaaacggg cgggcgatcc gtcactgttc ttctgatgac gcggagataa cacatcatgg
     8641 atcaaaaaca gattgaagaa attgtacgca gcgtgatggc gtcaatggga
//
LOCUS       DAJTYD010000160         8556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTYD010000160 DAJTYD010000000
VERSION     DAJTYD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.160"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(269..831)
                     /locus_tag="OO585_003289"
                     /pseudo
     CDS             complement(269..831)
                     /locus_tag="OO585_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1035..1241)
                     /locus_tag="OO585_003290"
     CDS             complement(1035..1241)
                     /locus_tag="OO585_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6630774.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1348..1911)
                     /locus_tag="OO585_003291"
     CDS             complement(1348..1911)
                     /locus_tag="OO585_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6630775.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3286..5121)
                     /locus_tag="OO585_003292"
     CDS             complement(3286..5121)
                     /locus_tag="OO585_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630776.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5222..5509
                     /locus_tag="OO585_003293"
     CDS             5222..5509
                     /locus_tag="OO585_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6630777.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5481..7010
                     /locus_tag="OO585_003294"
     CDS             5481..7010
                     /locus_tag="OO585_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6630778.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7180..8397)
                     /locus_tag="OO585_003295"
     CDS             complement(7180..8397)
                     /locus_tag="OO585_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6630779.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         2651 a         1934 c         1594 g         2377 t
ORIGIN      
        1 gctcgactga tttatcttta cgtcgcttac actgcaaatg ttcagaaatt agtctatgca
       61 gctcaccagt aaaatcagca atcagagaag gaattgcctt attcatacca gggcaaggta
      121 ttaatttaaa ctgtaataat ttaattttag gatgtgtagc agcagcccga tacagagttg
      181 caaggacaca cttttgccag agggcgttac aggaaagctt aatgtttgat tctgtataca
      241 taataaatca ccttatagtt acaataggtc aaaaaccgct gtagccaaag ttacgctggc
      301 ctgatgcttt agtgtcgagc ttcgtcaaat aatccagacg taccaataag cgctggtact
      361 gctctgggaa attaggatcg ttaatatcca ggatgtaacg tccattagca gggaaatgaa
      421 ctaatccctg gtgatcttcc agttccaacc ctaaggcact gtaccaggct ccggtaatta
      481 accctctcag ggtgtcagag cgctcatagt ttcccagatg gtaataggcg tctttgttaa
      541 aaaaaggcag acgtgataat gacatttaaa ggactgcgta tattctttcg cccagataat
      601 acaaaccgac gttgcgtagg tgcgtttgcc ttgttgtttt ttacggtgtt gatctgcatt
      661 aattttcgca ttcagcgagt tacggaaacg agatatctct ccggggatca ggttagggaa
      721 acaggtaatg atatccccgt tgtcaatcac aggagggtaa tggagatccc cccgtattgc
      781 tgttattctg gaagtgtttt taatattcgt attgatagtt ctcagagata tatcacactc
      841 tagatatttt aattaagtat cagttcgata tcggcatatt gtacactacc tcaggtagat
      901 gatgagatca gatacaggga tcaactgacg gtgagcgcag tccccacatt ttatccaggg
      961 cttactgcat attcccaaca gccattcgtt agccaggtag acgaataccc tgatttgccg
     1021 ctggttttgc tttcctatcg aagggcccat acatcatcac gcccacgaaa caaccgacga
     1081 aataacgcaa ctttctcatc cgtggataat acgaaaacgc actgcacagg gctctgcggt
     1141 ttacgtcgcc attcaatccc atgagcttca agtaatgaga ccagccgggt attttctgcc
     1201 cgcagtactg ctagttcatc gctgtcagat ttataaacca tatgcgttat cacttgtacc
     1261 tgtctcccgg cggcaccacg actgaaattc gtgtgaaggc aaatccacgc gataacttac
     1321 gatagttcgg acataagccc ccataattca gttatgtcgt ggtcgacccc ggttactttt
     1381 ttcgggaagc ctgctacggc ccagaataat accgtgttct ttctgatcct gtttagccag
     1441 atagctaagg gcgtaacgta agtcgtctac cgcagattta tcactatagt gaatcacgtg
     1501 atcgatacgc accgggtatt tgtctttagc ccggcacaga tgaaaataac cctccccttc
     1561 cgtgatccgc ctccagatat ctcccagttg cctggatatc tgataagagt tcttgtgacg
     1621 ttgtccatcc aggtagccga taaaatgaat atgaagccct ttgtctggtg tatattccat
     1681 tacccagtaa tatcccgcca acatcgtctg ggtttcgcta agtaaacggt atatttccat
     1741 acacatactg tgcttacagg aatgcccgaa actgggcgtg tctttcctgt aagcaaaatc
     1801 aattctgaaa ggtaacagtt tagaaaaacg ttgaaacata ccatccatgt gttcattcac
     1861 gtctttcaga atcatgaaat ccattttgta attgggattg gcattataca ttttatagtc
     1921 cttactttat aaaactcaca ggaagagaga attacgtaaa tactgagagt agagaaacgc
     1981 cgtaccacgg cattaataaa cgaagtaaac cagcagcaca gacaacctga taaaactgtt
     2041 ttgtattacc gcattactgt gatgaggtaa tacttcagaa tacagtgaga ctctcttaaa
     2101 ctgagaacca gtacgaatta caatggaaaa acacacctca tagaattgag tttcaggaag
     2161 caccactaaa ttctttacag gaagtaatct gacattcaca aaactcagta tcggctagct
     2221 acaggcaaca tcgctattaa tattgtcgaa ccattaagta aagccgagag agaccacggg
     2281 tatttaatca ttagcataaa agaataatgg tatacagaac atacccacac accataagaa
     2341 gattgttcca tacagaaaag atcatattta cctataccta taaactgtaa cggagtcata
     2401 acacgctcta atagtgttcc gatacgtaac cagtgttaag tacgtacagg acaacattac
     2461 acacataatt caccatacag cttcccccat atgcttcact gcgtagtgta tggagatata
     2521 tgcattacac acatacagaa tacttagcat aaagaggagt gacattacca cgtaaagaga
     2581 taagttatac aacaaacata caaacaatac ggcacagaca cacaatacca taactccaac
     2641 taataatact aattaacctt aattatttat ggtatatgaa ataaatgtga ttctcccgga
     2701 actaactaac ctaaccagac agatgatcac tgtataaaaa cttcagttaa tactcatttg
     2761 ttttgtcgac tcagtatata atgagtgacc agtaatcagg ataatcacac ttacataacc
     2821 tgtaaaaaag aacaagaaga ataagccact aacataatgc aaaatggcat ctgaatcgat
     2881 cgcagtttgg cattcgaatc gatcatgatt tggcccaatc cgtgatcgaa tagagcggaa
     2941 tacgcaacat aattcaccag acaagagctt acaccgaatt acagattatg tattaatgtc
     3001 aaaactggca tattttacag atacaatgaa catgcaaccc atcaatctaa taggggcggt
     3061 atgtacatct gcccatgaat gggatggcgc taagaataca aaacaattag acatagatca
     3121 caaaaacaca ccattctaat tgaaaaacaa aacaaacaaa taaatcatac tattatagaa
     3181 ttttctcttg ccagacatag ataatacccc tgctaatttt gctaatggtt agcaaaatta
     3241 gcagggtata taaagattat tgacccaaaa cacactcaga tgaattcacg acagctcagg
     3301 agggttattc ctgatgtcat cttcacaaat aatggttaac gcatcatata tcattctgcg
     3361 tctgttcctg ttttcggcct tatcgccata caacatcatc acatttttaa tttcatcgac
     3421 cggtgatttc acatagtgaa ctaatacacc aagcttctca acacattgca gtaaagtata
     3481 tttcttactg cctgtgaaaa gccctatcat agtttcttta cgttcgcgac tggataatac
     3541 tttgttttcc ataatgactt ctgccagcgt cttaccatca taaacatact ctttatctat
     3601 cagaatattt atcttatcca gcaccttctc tacgtcatca agcaaacctg taatatatgg
     3661 gggcgcaata tatggtttcg tactgatctt ttcaagcata caattataca tcttaataac
     3721 acgcatcagg gtaaaattgt tcgcatcata ctgtatttca ccctccttat atacaggatc
     3781 aataagtggt attttttcaa taaatgcatc ctgataggct tttacatggt caagccaata
     3841 aaacaacgag ccatacttta tattttttct atttacttta acctccagtg ctgtccgaag
     3901 caacgataat gcccccctga catatcctga aggtaattca ccaaacaatc cttttctttc
     3961 catgtcatca taatgctgca gcacatcatt aattttattt ttccttatca gacaaatgta
     4021 caccgcaagt gtttcgtagc agctggtcac atgaacagcc tcattctgca actgcccaaa
     4081 atagcttata atctttttca gatatgcgtc atcacaacca ttcttatcat taatattaaa
     4141 tacaagatct ttcagacggg aaatatattc ctgagattta tcatcaagtg aaacactata
     4201 acgagacaat gttccttctg cactgttttg aaacttataa aacatttcca gtaatttttt
     4261 tgcataaggc gttgtatcaa agctggtaaa gtcataaaga tattgcgcag cgtaaccata
     4321 caaaccatga atccagagtc cttctctcac caacactctg agataatatg caaggctttg
     4381 aagtatcttc gtctttcgtt gtatatcaag taagacactt aatgaataaa atgcattata
     4441 gaatttatca atattcctga gcactttctc cagttcatcc ttatccagct tcttctcccg
     4501 gtaatccttt ttatattcat ttttgatagc catgatatat tgttgcatca ggctcttgct
     4561 tttcccgtta tccttccgta tagttcctgt atatttcccc agagcataca gctttccatt
     4621 ttcatgaaaa tcttccttgt tgaatcccag ttcagccaga tattcccgaa gataacgttt
     4681 aacagcagct tcatcgcttt ctgtatcagc gtgatcaaga caaggacaat acgatttctg
     4741 taaaaaatta taaatatgat aataaaaagc agagtcattc tctggtctgt atgtattata
     4801 catatctaca acaagaaaaa taaccccaaa agtgcgcaga caatagctgc gaaggaattc
     4861 aggttttagt gtggaatgca ctggataaga attcatcatg taataaaaat gttcatatcg
     4921 ggcatatatg atccgccgtt gctcctgact gaacacccct tcagcatcaa taatacgact
     4981 gatctcatta taacgttgat cccagaattt ttgatgcatt tcaaaattgg tactcttccg
     5041 cttgtgtgcc ggattctcgc cttccttaaa caaccccaaa acagtacaaa tatcatacaa
     5101 catatttgta aatgtgcgca tatgctgtgc tctccggttg gtttgcattt tatcgcagag
     5161 gacattctga gcttaacgta tcaaactgac acaacgacat gcaatgcata tggagaacaa
     5221 tatgtcaaat caaaccggac agctttacac gccttcacgc atcctgcgta tggctgaact
     5281 tacccgactg ctgggtatat caagatctac tatttacgtg aaaataaatc cggcctcgaa
     5341 ataccacgac cctacctttc ccaaaccaat aaggcttggt agtgcatccg taggctggcg
     5401 ggcatctgcc atcgatgagt ggctgatgtt acatacggca ccagcctggt ctgaacctga
     5461 aaacaaacta aaggaactta atgatgacga caaaactatc actccctgaa cttgacacaa
     5521 ctaaactgcc tgacaggtgc caggcattac ttgatgaaat gcatgaagaa acaggaatac
     5581 gccgtgaaat acttctgtct gttatgctga cggtcatggc tgcctcggtc caggatacgc
     5641 atgaagttga actttccgga gggcaacgta ccagccttca gatatttatg tgtctttcat
     5701 cagcatcagg cagtggaaaa acctctgcct gcgcaaaatt aatcgctcct gttcatgaaa
     5761 cagaagaaga actacatcag gcatatatcg acgataaaaa aaactatgac cgaatgatgg
     5821 aaatgtggac aacagataaa aaaatcctgg agcggagata taaaaaggaa attgaaagat
     5881 ccccggaaaa tgctgctgct gcacgagcag cacttgaaaa gtgtatagca aataaacccg
     5941 aacctcccgt acaacaggtt ctcatcgtga atgacgcgac accagaaggg atagctttga
     6001 aactcagcca gtcaccctct cttctgttgc tgtctgatga aggaggtact attctggata
     6061 agcgtttcga acgcaaatcg gcactgtaca acacattatg gagtgggcaa ccagtcaacg
     6121 ttgaacgggc atccagaccc gggttccggc tgaatgatgt ccgtctcact atacttatac
     6181 tgactcaacc ggtaatattt aataagtttt ttactctcac tggcgatcag atccggggca
     6241 atggctttct ggcccggttg ctattttgcg aacccggagc aattaaaata atgacgacag
     6301 aaccgcatac tgccactcct gttgtgacgc aacaatgtgc aagctgtttt cgtaagaaaa
     6361 gttttggcag tcagatcaga gactctctga gagcatccag agaaagacgt gcaaaaagta
     6421 aagagcgcat ctgcatgaca ttatcaaatg cagcatcccg cgcactggag cgctttcaca
     6481 aagaaaatat ggatactgtc agacagaatc ctcatatggg tacatttgaa gacattattg
     6541 tcagaaaaag agagcaggtt gtcaggatag cagcacttct ggaactggag aaagatccag
     6601 actcgacagt aataacgctg gaaagtacta atagcgccat ttatctcatt gaattttatt
     6661 tcaagcatct tatatataaa cttgaatccc ttcgggaaat atccccggct gagaaacttg
     6721 ataaatggct tcagaaaaga attatcacaa cagcaggtta catattccag aaaagttata
     6781 ttttacaata tgccccatat gctctcagga aaaaatgtgt tctggatgaa gcacttgaca
     6841 tactggcgga acaaaggaaa ataaggattg atgataacct ggttgtttat ataggaaaca
     6901 cgattacacc atccgaactg gcaaaaaaac taaatattcc tgcattcgat gccggagttt
     6961 tcatttgtga tcatcaaaac atactgaagt atcaccgaaa cagactatag atactatcgg
     7021 acaaaaaata aattatcaca agtgtattat tcgttataat aatgaataat cgttatctga
     7081 gtgcaatgcc aggttttcat tacgaactgg cattgcattt atttttcaaa aaagaaagca
     7141 gtaaccattt ggctactctg agtatgccag tccatgaaac taagcgaatt ccttagtgtt
     7201 tttagcaagt tgagacatca cataatccgc ccatgcctgc atcatcggta cacgctgctc
     7261 cagtagatca gtacgatgat atgccgcctc aaccttattt ttcagcgtat gagcgagcgc
     7321 cctttccgcc agatcccgcg aatacccctg ttcactacac cagtccctga atgttgagcg
     7381 aaaaccatgt gccgtggcaa ctcgccccgg aatgtcactg acggctttct ttttacgcag
     7441 aaaacttgtc aacaccatat cggaaaggat ctgctgcttt ctgggtgaag ggaacaccag
     7501 ttcatcatgc aggccacgta tattttccag aatgtaaata gcctgccggg ataaaggaac
     7561 acgatgctgt agcctagctt tcattctttc tgcaggtata gtccataccc gcttatgaaa
     7621 atcaatttca gcccagcgca ttcccctggc ttcgcccgag cgagttgctg taagtatcac
     7681 cattaataac agtgcgcggg taacattata aggttcatca ctatacacac tggtcgccac
     7741 aaaaagcggt aactgcctcc agggcattgc gggttggtgt tcatcacgtc ctcttgtctg
     7801 ctgaggaagc aaatggtcaa ccacatcaac aggatttgcc acacaaaaac cgtgcgccca
     7861 tccccactgc ataacaacat gaatgcgctg tttaacccgg cttgccgttt ctgacaaggt
     7921 taaccagact ggacgcagtg tttctgccac gtccgcagcc gtaatcgaat ccagcgtttt
     7981 tgctcccagt tgaggaaacg cgtaattctc aagcgtcgat aaccactgcc ttacatgctt
     8041 tggattctcc catccaggag acagttctgc atgtacacgc ctggctgcat cggcaaatgt
     8101 tgggataacg actttctcag attcagcctt tttaatctcc agaggatcat cacctgcagc
     8161 aagttgctct cgcattatcc gggcagtacg tgcagcttca gcaatactga cctctgggta
     8221 agttcccaat ccagcattac gtcttttttg tgtcaccgga cttacataac gaaaaaccca
     8281 tttcccccgc ccctttactg aagaaggatg aagggtcagt ccggtaattc ccccatgggg
     8341 caatggtttg tcatcaggtt tgatatgtct tgctttcgta tccgtcaata ctgccatacg
     8401 ctaattcccg tcactctggt atgccatcca gtatgccatt agtccctcgc ttcactaaga
     8461 atacattaga taacatcgga caacaaaaag tgtaatacat tgtttataaa gaaattatat
     8521 aacacccctg gattaagtca gatttatttc aggcgg
//
LOCUS       DAJTYD010000161         8464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTYD010000161 DAJTYD010000000
VERSION     DAJTYD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.161"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            44..919
                     /locus_tag="OO585_003296"
     CDS             44..919
                     /locus_tag="OO585_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR6630780.1"
                     /translation="MFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVTVEDAVRG
                     ACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVWTDFHALA
                     LRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGMDFRAQAD
                     AIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVLKAYDIIS
                     HRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATMHALFNEP
                     LQGESANTSNIVEIF"
     gene            919..1515
                     /locus_tag="OO585_003297"
     CDS             919..1515
                     /locus_tag="OO585_003297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630781.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            1549..1950
                     /locus_tag="OO585_003298"
     CDS             1549..1950
                     /locus_tag="OO585_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6630782.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(2057..2938)
                     /locus_tag="OO585_003299"
     CDS             complement(2057..2938)
                     /locus_tag="OO585_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630783.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(2922..4571)
                     /locus_tag="OO585_003300"
     CDS             complement(2922..4571)
                     /locus_tag="OO585_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6630784.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(4864..5097)
                     /locus_tag="OO585_003301"
     CDS             complement(4864..5097)
                     /locus_tag="OO585_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCR6630785.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(5108..5296)
                     /locus_tag="OO585_003302"
     CDS             complement(5108..5296)
                     /locus_tag="OO585_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630786.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(5450..7864)
                     /locus_tag="OO585_003303"
     CDS             complement(5450..7864)
                     /locus_tag="OO585_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR6630787.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(7861..>8464)
                     /locus_tag="OO585_003304"
     CDS             complement(7861..>8464)
                     /locus_tag="OO585_003304"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCR6630788.1"
                     /translation="AYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHF
                     NIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVYCDPPYDGTFS
                     GYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFTHHYIKVKRSIG
                     VAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         2281 a         2094 c         1756 g         2333 t
ORIGIN      
        1 atccagtctg acccacagtg cttatccgtt ctggtccggt gcgctgttca accgtgggcg
       61 caacaaagct gacaaggtgg acatcgacct gtcccacagc aatctggccc ccggccggct
      121 gtgcgcagac gggcaatacc gccagatagt caccgtggaa gatgcggtgc gcggcgcctg
      181 taacctgttc gaccttgacc agttgcgcat ggagtacagc cctgacgaat accagaacct
      241 gctgatgtgc gagttcgtgg acgatctcgc gtccgtgttc ccgctcagcg aattgcaggc
      301 gtgcatggtg gacagctggg aagtctggac cgactttcat gcactggccc tgcgcccgtt
      361 tggctggcgc gaagtgtgga tcggttatga cccggcaaaa ggtacgcaga acggcgacag
      421 cgccggatgc gtggtggtgg caccgccagc cgtgccaggc ggtaagttcc gcattcttga
      481 gcgtcaccag tggcgcggaa tggactttcg cgcccaggct gacgccatca aaaaactgac
      541 tgaacagtac aacgtgacct atatcggcat cgactcaacc ggcgttggtc acggggttta
      601 cgagaacgtg aaagcgtttt ttcctgccgt ccgggagttt gtctacaacc ccaacgttaa
      661 aaacgccctg gtactcaagg cctacgacat tatcagccac cgccgtcttg agtttgacgc
      721 cgggcacacc gacattgcgc agtcattcat ggcaatccgt cgcgccacca ccgccagcgg
      781 caaccgcccg acctatgaag ccagccgcag cgaagaagcc agccatgccg atctggcctg
      841 ggcaacaatg cacgcactgt ttaacgaacc gctgcagggc gagtccgcca ataccagcaa
      901 tattgtggag attttttgat gggaaagagt aagaagaacc gcgctgcggc gacgaaacag
      961 acccagcata aaaaccagac ttcagccgaa gcattcagct tcggcgatcc cgttccggtt
     1021 ctggaccgcc gtgaactact ggactatgtg gaatgcgtac agacagatcg ctggtatgag
     1081 ccgccagtga gttttgacgg actggcgcgc accttccgcg ccgccgtgca tcacagttca
     1141 ccaattgcgg tgaaatgcaa cattctgatc agtacctaca tccctcaccc gctgctcagc
     1201 cagcaggctt tttcacgttt tgtgcaggat tatctggtat ttggtaacgc ttacctggag
     1261 aaacgcacaa accggttcgg agaggtcatc gctcttgagc ctgctctggc aaaatacacc
     1321 cgacgcggat tagacctaga tacctactgg tttgtgcaat acggcatgac cacgcagccg
     1381 tatcagttca cgaaaggaag catctttcat ctgatggaac cggacatcaa ccaagagatc
     1441 tacggcctgc caggttacct ttctgccatt ccgtcagccc tgctcaacga gtccgccacg
     1501 ctgttccgcc gatagtatta cattaacggc agtcatgcag gcttcatcat gtacatgacc
     1561 gatgccgcgc aaaaccagga ggatgtgaac aacctccgca atgcgatgaa aagcgccaaa
     1621 ggaccaggta acttccgcaa tctgtttatg tactcgccta acggcaaaaa ggacgggctt
     1681 cagatcatcc cgttgtcaga agtcgcggcg aaggatgagt ttctgaacat caagaacgtg
     1741 agccgggatg acatgatggc ggcgcatcgt gtgccaccgc aaatgatggg gattatgcct
     1801 aataatgtcg gggggtttgg ggatgtggag aaggcgagcc tggagtttgt acgtaacgag
     1861 ttaataccgt tacaaaaaag aatgatggaa ataaataaat tggtaagtga gaacattgtt
     1921 aaatttgata gttataaact agctacttaa cgtaatcact aaatgaggtt ctttaacaca
     1981 gctaaaaaat ttattaatag ccagcagttt ttatgaattt cttcagcgtg cagatacacg
     2041 ctgaagatgc aaaaaatcat atgagacgac cgtctggact tctgttacct aaatgtaatt
     2101 cacagagtat ccacctaatc acatcaggtc gaattaaccc agctacaaaa accgaattcc
     2161 aattattttt acctttatgt ttttcatcca acagcaacaa ctcgcgagtc aacaactcat
     2221 atacatcgct ataagtagaa agactcttat tcagtcctct aataacaagc tcaggatata
     2281 agtaaaacga ctcccattgt tcagacaaat accccttaat attattcttg ataacaacat
     2341 tatcaagatg atacttaaca ttgttatatt ctggtgacat tgggtcaatt atgattttta
     2401 catcaattgt aggtgttcgc cctaatttag aaaaattagc acatattaat ctttgtttta
     2461 atatattatc aaaatatgga tgcagcatcc gatggcctgc agaattagct atagtagtac
     2521 ttttgaaagt attacatttg caagcaggaa ccaaattctt agtgaataaa gaaaattctg
     2581 gatagttttc cttgggtaaa acatgatcca aagtcccgct atgcattgaa ccacacattg
     2641 gacatagaga gttagaatta gctgctctta tatcatcaat tatagataaa cactttgcgg
     2701 gattgttata ataatatctc aaagcttcag caatattgtt actcaatacg ccacaagaat
     2761 tgacattaag atacccttta ttctttatgt acgatctata tgcattaagt ataaatgtat
     2821 cctgtccatt cagaacagaa tgatgtttta atctcttatt tgcagatagt tttttaaatg
     2881 cagaataatc tttaaaaaca ggtcttttta atttattcat ttcaattgcc catttcattt
     2941 ctcaacgcag ttaatacatt aagtgataag tcctcgctat atttagattc aacatcatcc
     3001 caagacatat tctttgcaat tatattgttt tttactttat ttaaaatttg agttggttca
     3061 ctctcaccga aaacaaaata tgaaatattt cctatattcg ccccaaaagt actcaatctc
     3121 ggtttttcgc taacaattac cccatcatct cgtcttctga gtattgtcac ttgctcctta
     3181 aaaacctccc gaacaagata aaccgaatgc gttgctatga tagctgaaga gcctgtcttt
     3241 tctaataaat tatcaagcaa tgccataaat ttattaataa atgaaggatg gagatgtgtt
     3301 tctggctcat ctattaatag aagagtccca ttctcaatat acaaacagac ttgtacacaa
     3361 aacttaataa acgatatttc cccactactc aaagggtaac atttattttc aataagcctc
     3421 aaaggctcgc cagagagaat aatttcagaa tataatgata gtctagattc ttcgcctcga
     3481 gaattaagct catttaattt aacaaatggt gaattttcac cgccacattt gattactaaa
     3541 tcgttgttac tatttagaac actcagtgca tcaacaaata tattccacct ttgattatag
     3601 ccgatagcgt cgttactcct cgctagttgc actattaaat cattcaagtt taatttggat
     3661 tttgattcac gatttaaagt caatcttttg taccatatca atgattttat tcttttatcg
     3721 gttggaaatg aacacttact ttcatttgtt ggagaaaaag caatgagcct actaatttga
     3781 attcgttctt caatgctcac accgttagaa tcaactacag ccaccaaatt atcctttcct
     3841 ttaatcgcag ccaatgcgat ttctctaagc gtttggcttt ttcctacacc attctcgcct
     3901 ataattatgg aaattctttt tggtaaatca tgctcgtgat caaaattaaa aacataagat
     3961 acatcttctt cattaacttt attcgaatag ctaatcttga tgtgctgtga taaattaccc
     4021 aactcctcat attgaatacc ggaaagaact gttcctgcat ttttgaaagt aaaatacgac
     4081 tcagactctc taatgaaaga tttattaaag atatcagaag agattgcttt tttcctcaaa
     4141 ttctctgcac tagcgtcact tttcttagaa acaacatcat taatgaaatt taatatatat
     4201 ttggattcat tcaccccaaa aaatcttact agtcttcgat aattattcat atcctgaagc
     4261 atgacaaaat aatcattaaa cccctcatcc tttaaattag attcttcctt tttcttcact
     4321 cgccttatca gttctgcttt tccatttcgt tcctcctctg aattcaaaaa accaatataa
     4381 ccattcagtg aaattatatc cttacactct tttttattaa aaatagatat ttcgactcta
     4441 ctcaaatatc caaaatcatt ccaatcgctt cttgatggga ctatttttat ataattacca
     4501 gaatcagata tctgttgatt atttgtaatt aaaaaatata aactataatt tttatttttc
     4561 ttgactaaca ttattaccct ctcacttcat tagtgatgat ttattttgga attattttac
     4621 acacttatac ctagcgcgca atcgtatccc cgccacgcct gcccgcttta tgtagaggtt
     4681 ttcatgcacc tgcatgatct acgcaaaagc ccgccagttc tggcgggcct tagcaaaaac
     4741 gatcctcaaa cgatcatgcg atctcatgcg gcatagacat gcactacaga gctaacgcct
     4801 cgcaagggct cgttgttcaa ccttgctgac gccagaagct agttcagacg ccagcaacgt
     4861 ttcttaatgc agccagctgt cgtcttccca caccttctgc ataattttca tcacttgttt
     4921 tctttcttcg tccagttgca atccagtaag ttccacaccg ttagagctac ctttgcggat
     4981 acgaattacc gttttgggat acagagggcg cagattgcgg taaagctcgg attcaagagc
     5041 gtccagggta gactggctaa tcttctgctc tttatcgatc attatttcaa tgcgcataaa
     5101 agtcacctca gctgatgaca tccattgagc ggttgtattc gtgggttctg atttttgcca
     5161 tgagttcatc agtcaattca gaaacccact gcagagccag ccccttctct tcatcactac
     5221 actcactagc cgctacaagc ttaagaaaaa aatcaatgcg ctggagcttc aaagactcca
     5281 aaaaatagtc ctgcatcttt cctcctatga caccacacgc aatactgtat gcataaccac
     5341 tgtttatatt tacagcatat aataatctta ctgatgtaaa acgttttttt acgttcatca
     5401 gcctgatatg cctggtatta ttaagagcac gaattgttaa cccgcgtaat taatacaggt
     5461 tccgccactg atcatcttcc tgcaaacgct ggttccgata gaagatacgc aggcctgctc
     5521 ctgacggaat actgcctccg cgaaggagta aatcgacctc tttctcgctg ccatcaaatc
     5581 ctctggactt cagctcatac acgagctgca gtcgctgatg gtctgtaatt cgctgtttgt
     5641 agtctttacg ccgtttcggt ttcaccaggc gtaaccttgc tgccagttcc cggcgctctt
     5701 ttttgctcat actgtgcagg taatcgtgca actccttgtc atccatgctg gtaatgtccg
     5761 ttctggggtt cccatcagct gatttatctt tctcctgttg gttcaaattt tcagcaagga
     5821 gacagttatt gccacgagtc caaggggcgc aagcgccctg gtcggctgcc gcctcctgaa
     5881 cgtcaacggc tttacgaacc attttccact tcactgcatg agtgcagatc ttgccttctg
     5941 caatgggtga ccagatgcca taaatacgaa taccgtgatc gccataggcg gtcggctctt
     6001 cattgatttc ataagcggtt ctgatcaagt gatatttgcg aggaaccagc acgccgccct
     6061 gcctcataat gtaggtggca aaacagccag catcagcagc agccaggatg gcatcaaggc
     6121 gcgggttatc cagtaccggc gcacctgctt ttttgtcacc ctgctgcctt gccgcctgac
     6181 cagccaacaa ccgcagttca cggtaagcct gacgccctgg aataccaaag aagcggaatt
     6241 gctgaacacg atgcagagac gcccagggat taacgtattc agcgttatca cgcagggatt
     6301 tacccgtttc cttgctgatc tcgccagcca gaccacgccc gtcaatgttc ttactgatgt
     6361 atttcgcgat gtagcttgtc ggcgttcctt tgcgcgggtt tatcagctca gatttaaagc
     6421 gtggaccagt gttattaccc agttcctcgc ggtcttcacg gatggcaaac ttacgcaaca
     6481 atgcagtaat ggcgcggcgg tcttttttgc gcataaaaca caacaggtgc cagtgaactg
     6541 taccgtcatg atgcggctca gccacccgca cgccatacca gcgcaacccg gctttgtgca
     6601 tcgccttacg aaatgcagca aacatgccga ccagataatc gctgctttgt cttaccgtcg
     6661 catttgtcca ggttgggttt ggtctgccgt tattgagcgt ggaatggaaa cgtgacggac
     6721 aggtaatggt gtagaaaacg gcgcagtcac cgcgcatttc cgcgataagc tccagacctt
     6781 taacacaggc catcatctca ttgcggcggt gcgccgggtt gctgctgctg gcgtttacca
     6841 cgtcttccat atccagcgtg tcgccctctt cgttcaccag ttcatgagaa cggaaaaact
     6901 ccaatgactt gcggcgctgc tcacgtttat gcatcacggc ttcatagctg acatagggag
     6961 atgctttttt gctgaccagg cagacagcac gcaactgctc ttcccgccat tcgcaacgca
     7021 ttttccataa tttccggtac caccagtcgg cgcacaacat acgcgccagc gaacccggaa
     7081 tgagttcata gggcacgggt ttacggcggt ttcttttccg acggagttgc tcaaacgcag
     7141 gcgggatgac atccagacgc agggtttccg ctgccacctt ttcccatgtc ttgcggattt
     7201 cttctggctt aacgtcatcg gtggcataca aatcaccaca agctgcatca aggcacatgc
     7261 tcatatgcgc agcgacaagg gtagacaggc gtttcacctg atcctgactc atttcaggca
     7321 gaatcagcag gccgtccagc cctacatggc ttgccataaa acgaaaagat gcagatagct
     7381 ggctgtcgcg tacatgctcc agtcgttcca gacatggctt aatcgtctca cgtaaatagc
     7441 gggaataagc ctttggcctg cccaggctgc tgaagtattc aatacgttgc atcagcggct
     7501 tgctgatatg ggaaggctgg gcgttgacgt ccgccagaat gaccatatcc ggattaaaac
     7561 gctgctgctc atgcgccagc tttgcccggc taatgagctt atcctgctcc atttcgtgct
     7621 ggacaggatc acgtgattca taaaaaaaat aacgctccca gacctgatca ctcagtgcct
     7681 cgcggcgcaa ctgttcctgc tcgttatcgg cagcgtacag agtgatcagg tttgaaagcg
     7741 cagaaaccgg cgcaacttcc gccgggtcca gataagggtt aatggccttt ttcgggctgt
     7801 tccatgagaa cgctgcggca gcctcgttaa agccgcagca gttgttcata tcggcatggc
     7861 tcatgcacgt actccgtaca cggcagaact atccacgcca cgcgaataat caaatcccac
     7921 ccagcagcgc ggcccggaaa cagcaatgat ttctgttgct gatttaccct cgccagctgc
     7981 cacaccgatg ctgcgtttta ccttgatata gtggtgagta aaattgcgat acagcgaacg
     8041 gatcagggat gtgtcactgt tagaaacaat gaccggatgt ccttctgatg accgatgttc
     8101 aagaacggat gctaggtgat actggtcatc ttcagtgaaa ccatcagtgt gatagccgga
     8161 aaacgtaccg tcatacggtg gatcgcaata caccacatcc cccgccttca acatcgccag
     8221 cgtttcatca aagctggcgc agataaacgt tgctcgctgg gctttttctg caaatgcgcg
     8281 aatttctttt tcagggaaat acggattttt ataattgccg tagggaatgt tgaaatgccc
     8341 gctcttgtta tagcgacata aaccacggta accgtgacga ttgagataca ggaaatatac
     8401 cgctttcatg aaatcagtaa tttcagttga gtaattaaac tcctgcctta tgttgtaata
     8461 agcc
//
LOCUS       DAJTYD010000162         8340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTYD010000162 DAJTYD010000000
VERSION     DAJTYD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.162"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..66
                     /locus_tag="OO585_003305"
     CDS             <1..66
                     /locus_tag="OO585_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR6630789.1"
                     /translation="RYQAKYVSAIWQKLIPSQADF"
     gene            125..328
                     /locus_tag="OO585_003306"
     CDS             125..328
                     /locus_tag="OO585_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR6630790.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            328..1353
                     /locus_tag="OO585_003307"
                     /pseudo
     CDS             328..1353
                     /locus_tag="OO585_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1381..1470)
                     /locus_tag="OO585_003308"
     tRNA            complement(1381..1470)
                     /locus_tag="OO585_003308"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1434..1436),aa:Ser,seq:cga)
     gene            1564..2361
                     /gene="mtfA"
                     /locus_tag="OO585_003309"
     CDS             1564..2361
                     /gene="mtfA"
                     /locus_tag="OO585_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6630791.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2462..2537
                     /locus_tag="OO585_003310"
     tRNA            2462..2537
                     /locus_tag="OO585_003310"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2495..2497,aa:Asn,seq:gtt)
     gene            2851..>8340
                     /locus_tag="OO585_003311"
     CDS             2851..>8340
                     /locus_tag="OO585_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6630792.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYILGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFNNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPSWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLKALNTNENGIAEVTLKGTVLGVYTAEAILLNGKSDTKIVNIVPDTSNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTKPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLPNGNGYTTTVNIAPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNGVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGETTSLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQLTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLK"
BASE COUNT         2392 a         2002 c         2023 g         1923 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgag tcaggctgat
       61 ttttaatgtt caaccctaat tgccgctaac cgtatatagt tagcggcggt tatgagatat
      121 agctatgagc agcttatttt taaccgaaga tgaattgcta atattaacgg gctgcaaata
      181 tgcaagccac cagcgaaaat ggttaatgga aaacgagctt ccgttctata ccaatcgtag
      241 tggcaaaccg attgtcagcc gggatctatt tacctgcaat aaaactttac caccacgcga
      301 ggtagagccg aattttggtg cgatctgatg ggaagacgaa ggaaaaatcc tgagcacgaa
      361 aaattacctc caaatgtata cccaaataaa tatagttatg tatggaaacc aacatccagc
      421 gaatctgtaa cactaaccgc catcaaggat ggtttagctg ctttatggaa aaagtatgag
      481 gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc gcttgtggga aaaattcctc
      541 gccagcgcct attacagtga cctcagtcca agaacacaaa aagattatct gcaacatcaa
      601 aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca gaacacatcc
      661 gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat gaagtcccgc
      721 gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc aggtgtaagt
      781 aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca ggcagtatta
      841 agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg tgcagcgagg
      901 gtttccgatg tgttatcgct gaaatgggag cagattggaa acgacgggat ctttatccag
      961 caagggaaaa caggaaaaaa acagataaaa gcatggagtc cacgattaca ggcggcgatc
     1021 gaaaaagcaa aacagttacc aacatccgcc tatgtaatca gcaatcaata cggcaaccga
     1081 tatatgtaca aaggctttaa cgaaatgtgg gtagaagcaa gaaatcgcgc aggcaaaatt
     1141 tcaggtattt taaccgactt cacctttcat gatctgaagg cgaaaggaat ttcagactat
     1201 gaaggaagca gtcgggataa gcaacttttc tctggtcaca aaaccgaggg gcaagtgcta
     1261 atctatgaca ggaaggttaa agtttcaccg acacttgatg tcccgttacc tgaaaatatt
     1321 ccaagtaatt ccacgtgtga tttttgtcac tgacttaatg atgtataagt gattgaattt
     1381 tggcggagag agggggattt gaacccccgg tggagttgcc cccactccgg ttttcgagac
     1441 cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat
     1501 ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa
     1561 acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg
     1621 caggaagcac tatcgatccc ccttttaacg tgtctgacgg aacaggaaca aagcaaatta
     1681 gtcactcttg ccgaacgttt tttacagcaa aagcggcttg ttcctttaca gggctttgaa
     1741 ctggattcat taagaagctg ccggatagca cttctatttt gcctacccgt tctggagtta
     1801 ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc
     1861 gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt
     1921 cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat
     1981 gcttctggtt ttaacctgat tattcatgaa gttgctcata agctggacac ccgtaacggc
     2041 gatcgcgcca gcggagttcc ctttattccg ttgcgtgagg ttgctggctg ggaacacgat
     2101 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tggttggcga gaatgcggcg
     2161 agcattgatg cttatgcagc cagcgatcct gctgaatgtt ttgccgtact ttctgaatat
     2221 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgc
     2281 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt
     2341 tcggcgacga atgttcatta attaacaact ttgcatatta attaatcaat tgaaatgact
     2401 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttcacacga
     2461 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     2521 tccagtcaga ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc actgacctta
     2581 gttgaacatt gttttttaac ggatagcggg tttttaacat cttaagcgcc ctcgaccttt
     2641 atggttgagg gcgttttgct atgaacgcca tcaccatttt cccctcgatt ataaaacttg
     2701 agttattcag tagtctcccc tcttgcaact cacacccaaa actgcctaac gaaaagttat
     2761 taattttcaa tcatattgct atcagtattt acattttttc gctgtgctag aaagggcgca
     2821 tttatgttag ctcgttcagg gaaggtaagc atggctacga agaagagaag tggagaagaa
     2881 ataaatgacc gacaaatatt atgcgggatg ggaattaaac tacgccgctt aactgcgggt
     2941 atctgtctga taactcaact tgcgttccct atggctgcgg cagcacaagg tgtggtaaac
     3001 gccgcaaccc aacaaccagt tcctgcacaa attgccattg caaatgccaa tacggtgccc
     3061 tacatccttg gagcgctgga atcggcccaa agcgttgccg aacgtttcgg tatttcggtg
     3121 gctgagttac gcaaactcaa ccagtttcgt acgtttgctc gaggttttaa taatgtccgc
     3181 cagggtgatg aactggatgt cccggcacaa gttagtgaaa aaaaattaac cccgccgccg
     3241 ggtaatagca gtgacaacct cgagcaacag atagccagta cttcacagca aatcgggtct
     3301 ctgctcgccg aagatatgaa cagcgagcaa gcggcaaata tggcgcgtgg atgggcctct
     3361 tctcaggctt caggcgcaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg
     3421 ctgggcgtgg atgaagattt tagcctgaag aactcccagt tcgattttct ccatccgtgg
     3481 tatgaaacgc ctgataatct ctttttcagt cagcatactc tccatcgtac tgacgagcgt
     3541 acgcagatta acaacggctt aggttggcgt catttcactc ccacatggat gtcaggcatc
     3601 aacttctttt tcgaccacga tcttagccgt taccactccc gcgccggcat tggcgcggag
     3661 tactggcgcg actatctaaa attaagcagt aacggctatt tgcgactgac caactggcgc
     3721 agcgcacctg aactggacaa cgattatgaa gcacgcccgg ccaatggctg ggatgtacgc
     3781 gcagaaagct ggctaccctc ctggccgcac cttggcggta aactggtcta tgaacagtat
     3841 tatggcgatg aagtggccct gttcgataaa gacgatcggc aaagtaatcc tcatgccata
     3901 accgctggac ttaactatac ccccttcccg ctgatgacct tcagcgcgga gcaacgccag
     3961 ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt ttacctggca acctggcagc
     4021 gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac ggcgtagcct tgcaggcagc
     4081 cgttatgatc tggtggatcg caacaacaat atcgttctgg aatatcgcaa aaaagaactg
     4141 gttcgcctga ccctgacaga ccccgtgaca gggaagtcag gagaagtgaa atcactggtt
     4201 tcgtcgctac aaaccaaata tgccctgaaa ggctataacg tcgaagccac cgcactggaa
     4261 gctgccggtg gcaaagtggt cacaacgggt aaagatattc tggttaccct gccggcttac
     4321 cggttcacca gtacgccaga aaccgataac acctggccga ttgaagtcac cgccgaagat
     4381 gtcaaaggca atttgtcgaa tcgtgaacag agcatggtgg tcgttcaggc acctacgcta
     4441 agccagaaag attcctcggt atcgttaagt acccaaacat tgaacgcgga ttcccattca
     4501 accgccacac tgacttttat tgcgcatgat gcagcaggta atcctgttgt cgggctggtg
     4561 ctctcgacgc gtcacgaagg tgttcaggac atcacccttt ctgactggaa agataatggt
     4621 gacggaagct atacccagat cctgaccaca ggagcgatgt ctggcacgct gacgctgatg
     4681 ccacagctga atggtgtgga tgcggctaaa gcccccgccg tggtgaatat catttctgtt
     4741 tcgtcatccc gaactcactc gtcaattaag attgataagg accgttatct ctccggcaat
     4801 cctatcgagg tgacggtaga actgagagat gaaaatgaca aacctgttaa ggaacaaaaa
     4861 cagcaactga ataacgcagt cagcatcgac aacgtgaaac caggagtcac tacagactgg
     4921 aaagaaaccg cagatggcgt ctataaggcg acctataccg cctataccaa aggcagtgga
     4981 cttactgcga agctattaat gcaaaactgg aatgaagatt tgcataccgc tggatttatc
     5041 atcgacgcca acccgcagtc agcgaaaatt gcgacattat ctgccagcaa taatggtgtg
     5101 ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac
     5161 ccaatcaatg atcataccgt cacgtttgcg gtattaagcg gatcggcaac ttccttcaac
     5221 aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga cttttgatct gaaaagtagt
     5281 aagcaggaag acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc
     5341 gtcagttttg tcggcgactc gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa
     5401 gtggttgctg acggcaatga cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc
     5461 aacctgctca atgacgtcat ggtcactttc aatgttaatt cagcagaggc gaaactgagc
     5521 caaaccgaag tgaatagcca cgacgggatc gccacagcta cgctgaccag tttgaaaaat
     5581 ggtgattata gggttacggc ctctgtgagc tctggttccc aggctaatca acaggtgaat
     5641 tttatcggtg atcaaagtac tgctgccctg accctcagtg tgccttcagg tgatatcacc
     5701 gtcaccaaca cagctccgca acatatgact gcaaccttgc aggataaaaa tggcaaccca
     5761 ctaaaagata aagaaatcac cttctctgta ccaaacgacg tcgcaagtcg gttctcgatt
     5821 agcaacagcg gaaaaggcat gacggatagc aacggggttg caatcgcctc cctgaccggc
     5881 acgttagcgg gcacgcatat gatcacggct cgtctggcta acagcaatgt cagcgataca
     5941 cagccaatga cgtttgtggc ggataaagac agagcggttg tcgttctgca aacatcgaaa
     6001 gcggaaatca ttgggaatgg cgtggatgag acaactctga cagcaacagt gaaagatcct
     6061 tttgataacg tggttaaaaa tctgtcagta gtcttccgca cctcccccgc agacacgcaa
     6121 ctgagtctga aggcgcttaa tactaatgag aacggtattg ctgaagttac ccttaagggc
     6181 acggttttgg gtgtttatac agccgaagcc atactgctta acggtaaaag cgatacgaaa
     6241 atcgtcaata ttgtgcccga taccagcaac gcgcaggtca ccctgaacat cccagctcaa
     6301 caggtggtga cgaataacag cgacagcgtg cagctgacgg cgacggtgaa agatccgtcg
     6361 aatcatccgg tggcgggaat aacggtgaac ttcaccatgc ctcaggacgt ggcggcaaac
     6421 tttacccttg aaaataacgg tattgccatc actcaggcca atggcgaagc gcatgtcacc
     6481 ctcaaaggta aaaaagcggg catgcatacg gttaccgcaa cgttgggtaa caataatgcc
     6541 agcgatgcgc aacctgtcac attcgtggcg gataaggaca gcgcagttgt cgttctgcaa
     6601 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctaac agcaacagtg
     6661 aaagatcctt ttgataacgc agtaaaagat ctacaggtta ccttcagtac caagccggca
     6721 gatactcaac ttagtcagag cacaagcaat actaacgaca gtggtgttgc tgaagttacc
     6781 cttaagggca cggttttggg cgttcataca gtcgaagcca cacttcctaa cggcaacggc
     6841 tatacaacga cagtcaatat tgcgcccgat accagcaacg cgcaggttac gctgaacatc
     6901 cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacggc gatggtgaaa
     6961 gatccgtcga atcatccggt ggcggggata acggtcaact tcaccatgcc acaggacgtt
     7021 gcggcaaact ttaccctcga aaataacggc attgccatca cccaagccaa tggggaagcg
     7081 catgtcacgc tgaaaggtaa aaaagcgggt acgcatacgg ttaccgcaac gctgggtaat
     7141 aacaatacca gtgattcgca gccggtaacg tttgtggcgg acaaaacctc ggctcaggtt
     7201 gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact
     7261 gcaacggtta aagatcagtt cgacaatggg gtgaataatc ttccggtaac attcagctca
     7321 gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg
     7381 caggccactc tcgcaggcgt tgcctttggt gagaagacgg ttactgcatc actggctaat
     7441 aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt
     7501 atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc
     7561 agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg
     7621 aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc cgtgacgaac
     7681 gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg
     7741 agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt
     7801 aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca
     7861 gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt
     7921 gtcccccagc aggaggtaac cctcagcgtt tcaccaagtg aaggcgtgac ccccagtaat
     7981 aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac cgctacaaaa
     8041 gccggggttt atcaattgac ggcaaccctc gaaaatggcg attcgatgca acaaacagtg
     8101 acctatgtgc cgaacgtcgc gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg
     8161 attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac agagggcaat
     8221 gcgatagcca acactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg
     8281 ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt cacgctgaaa
//
LOCUS       DAJTYD010000163         8325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTYD010000163 DAJTYD010000000
VERSION     DAJTYD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.163"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            129..371
                     /locus_tag="OO585_003312"
     CDS             129..371
                     /locus_tag="OO585_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630793.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(701..1630)
                     /gene="fdhE"
                     /locus_tag="OO585_003313"
     CDS             complement(701..1630)
                     /gene="fdhE"
                     /locus_tag="OO585_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6630794.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1627..2262)
                     /gene="fdoI"
                     /locus_tag="OO585_003314"
     CDS             complement(1627..2262)
                     /gene="fdoI"
                     /locus_tag="OO585_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6630795.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2259..3161)
                     /gene="fdoH"
                     /locus_tag="OO585_003315"
     CDS             complement(2259..3161)
                     /gene="fdoH"
                     /locus_tag="OO585_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6630796.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3174..6224)
                     /gene="fdnG"
                     /locus_tag="OO585_003316"
     CDS             complement(3174..6224)
                     /gene="fdnG"
                     /locus_tag="OO585_003316"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5637..5639),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6630797.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6418..7251
                     /gene="fdhD"
                     /locus_tag="OO585_003317"
     CDS             6418..7251
                     /gene="fdhD"
                     /locus_tag="OO585_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6630798.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7405..>8325
                     /locus_tag="OO585_003318"
     CDS             7405..>8325
                     /locus_tag="OO585_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR6630799.1"
                     /translation="MKRNLLSSAIIVAIMALSLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         1963 a         2134 c         2198 g         2030 t
ORIGIN      
        1 aactgttaca tcgtatctgg aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc
       61 tttctttaaa gattcggcaa gggtactttt ctctccatag attcaaaaat gatattggcg
      121 agatatttat gaactctctg gcaggtatag atatgggcag aattttactc gatttatcga
      181 atgaggtgat taagcaactt gatgatctcg aggtgcagcg taatcttcct cgtgcggacc
      241 tattaaggga agcggtagat caatacctga taaatcaatc gcaaacagca agaaccagtg
      301 ttcctggcat ctggcaaggg tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg
      361 aggaatggta atgggaaaaa atgatgtcaa tcagattgct gacaacgtgc gcgttgttca
      421 tgccggatgc ggcgtgaacg ccttatccgg cctacaaagt catgcaaatt caatatattg
      481 aagtttattg gtaggcctga taagcgcagc gcatcaggca atcatacgct tgtcatcagt
      541 gacataccca taaccttatc gatgtgttgg agggaagaac gaggcaaaca gacaattgcg
      601 gcatgatctt gaatgggtaa cgcggaatac gagagatggt gtcgtcacac cctatgagct
      661 gtaatccggg cgaccagcgc cacccggaaa acgtcagaga ttactcccct tcacccggaa
      721 acaggaacgg gttgatggaa ctgcgggcat agccttcttg ctccattcgc gcgtccagta
      781 ccagagaagc gaggtcatct gccacggctt caactttcgg ttctttttcc tgataaagaa
      841 tcttcaggta agtgccgcag tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat
      901 ccagcgacca gtaatgcagt ttgccgctct gttcacagtt gctgcatttt acgcgcacta
      961 cgtgccattc ggtttcacac aggttgcagt gcaggtaacg cagaccctga gtggtgccaa
     1021 tttgcaccat gctggacacc ggcatagaac cacaaaccgg gcaatattga cgttgctcgc
     1081 cgtattcagc gcgggctttg ccggggatca gattggccat ctgcgcccag tagagcgaca
     1141 gtgcagccca gataaacggc gctttatcgc tgctgacgga tgagaaatca gaggcaaaca
     1201 gtgcgctggc catatcttcc agctcctgag tcgatgcctt ctccagattc tcaatcactg
     1261 ccagcgccgg gccgctcatt tcaggtttca gctcagcaat cagcgccatc agcagctttt
     1321 gccagtgctt atcacgcggc agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg
     1381 cttctttaat gcgcgcggtc agatccatct ccagcggatg gtcgtacagc accacttcct
     1441 gggcgtgggc gataagcgca gcaaagcgca ggtaatcatc cagcggatta ttttctgcca
     1501 gctcgcgcag acgctcggcg cggcggttgt ataaattctt gagccgaggg aacaataacg
     1561 gcggaatcat atccgccgta cgtttctcgc tcgaacccag ctcatcttgc gggattatgc
     1621 gaatactcat tcagctttct tttccgttgt cttgcggacc tcacggtacc agcgcgggtg
     1681 atgtttcttc gcccaggcgc tggtaaccca tccttccacc atggcggtaa tcgtgccttt
     1741 cacccaaagg gcggcgtaga tatgcaccat gataaccaca attaacgcca ctgcggcaaa
     1801 tgaatgcagc attaacgcga atcggatcac cgggattgag aaagcaggcg caaaataagg
     1861 acgccagata atcacgccgc tcaccagcag cagaaccagg aaaataatcg ccgcccagaa
     1921 aacgcatttc tgaccgaagt tataacgccc ggtgtcacct acttcctcgt tgacgacgat
     1981 cttacgaata ttcttcgccc aaaagatatc atcccgattg attaggttgt gatgccagta
     2041 acggaaaaac atgatgatga acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat
     2101 tcgcgccagc tgcggtgtgc ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa
     2161 gcccagcccg ctcaccgccg ctaggatgaa gcagaaggcg gtgatccagt ggttgatacg
     2221 ttccggcgcg gtgtagcgca cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc
     2281 tcttcgtgca gattattctc ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg
     2341 aagatactgg ctgcgaaggt agccgcaaag ccaacagctg cgagcggttt ccagatgcct
     2401 ttccagaatt tcacggtttc gctgatttcc gggttctccg gcaagccatg atacagattt
     2461 ggcttgtcag catggtgcag cacgtacata acgtgtgtac caccgacgcc tgccggatcg
     2521 tacaggcccg cattgtcgta accgcgggtt ttcagctccg ccacgcgctc gctcgccagc
     2581 gttttcatcg actctttcgt accgaagtga atcgcgcccg ttgggcaggt cttcacgcag
     2641 gctggttctt gcccaaccac cacgcggtca acgcacagcg tacatttgta gacgcggttg
     2701 tcttccgggt tgaggcgcgg aatgtcgaac ggacagcccg caatgcaata accgcagcca
     2761 atgcactgct cggactggaa gtcgacgata ccgttggcat actgaataat tgcaccttcc
     2821 gccgggcacg ctttcagaca gcctggatcg gaacagtgca tacagccgtc tttgcggatc
     2881 agccattcca gtttgtcgtt ctgctccact tccgagaagc gcatcaccgt ccacgatttg
     2941 gcacttaaat cattggggtt gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg
     3001 tcgttccact ctgaacacgc cacctgacag gctttacagc cgatacaggt ggtgacgtcg
     3061 atgagtttcg ccacttcttc ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta
     3121 gtcgcggaac gacgaatgat atcttgagat tgataagcca tatgtcgtct ccgttacacc
     3181 ttttccacat tcacaaggaa ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg
     3241 aatggcgtta aagtattggc aataaagcct tttttagcta cgccttcata gccccagtga
     3301 atcggaatac cgatggtatc gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg
     3361 gtcaccaccg ctttggcttt gatatagcca cggttggagg agactttcac ggtatcgccc
     3421 tgggcaatgc caagtttatt cgccagcgac tccccgattt ccacaaactg ctctggctgc
     3481 aaaattgcgt tcaacagcgc gtgtttggtc cagtagtgga agtgctcggt cagacgatag
     3541 gtggttccga catacgggaa cttatcggct ttacccaatg cttcggcgtc gtctttaaag
     3601 atacgtgcag ccgggttcga gataacgttt ggatgcagcg ggttagttcc cagcggcgtt
     3661 tcaaacggct cgtagtgttc cgggaacgga ccttccgcca tcttatcgag ggcaaacaga
     3721 cgccccatgc cttcctgctg catgataaac ggcccgacgc cgctacccgg aggcgctgcg
     3781 ctgtagtccg gaatatccca gccggtccac ttagtgccgt cccatttcag caactgacgc
     3841 ttcggatccc acgggttacc ctgcggatct gcggaggcgc ggttatacag aatgcggcgg
     3901 ttaagcggcc atgcccatgc ccagcccagc gtgttaccga ggccagacgg atcggcgtta
     3961 tcacgacgcg ccatctggtt gccttccggc gtccagctac cggcgaaaat ccagcagcca
     4021 caggaggttg taccgtcatc gcgcaattgg gcgaacgagc taagttgttg gcctttcttg
     4081 acgataaccg caccggttgc cggatcggta atatcggcca gcgccttacc gttgctctcc
     4141 atcgctactt cttccgactt cggctcatgc ggaatggcgt agttccaggt catgttcagc
     4201 acctgatccg ggttcgcgcc accctgttcg gcatacatct tgcgcaagcg caggaagata
     4261 ccggagagga tctcaccgtc agtcagcgca atccccgggg cgtccgcacc tttccagtgc
     4321 cactgcaacc agcgacctga gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat
     4381 ggcagacgga acacttcggt ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg
     4441 ttctgccaga agttagaggt ttcagtgttc agcgggtcga tggtgacgag gaacttcagt
     4501 ttcgacagac aaccgatcac tttgtttttg ttcgggaatg aggcaacagg gttaaaaccc
     4561 tggcagatat agccattgac cttgccctct ttcatcatct cgaagtattg caggacgtcg
     4621 tagcctttat cccacttcgg caaccagtca aagccccagc tattttccgc cgtcgcttta
     4681 tcaccaaaga aggccttcat catagagacg aagaatttcg ggtagttgcc ccagtagttt
     4741 acctggcctt ccagcagcgg ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc
     4801 tgcttctcgc ttggcagcgt catgtaacct ggcaggctct gcgacagcag ccccaggtcc
     4861 gtcagcccct gaatattgga gtgaccgcgc agggcgttaa cgccgccgcc tgccatcccc
     4921 atattgccga gcagcagctg gatcatcgcc atcgtacgaa tgttttgcgc accaacggag
     4981 tgttgcgtcc agccgagggc atacaggaac gaggcagttt tatcgtgagc actggtttct
     5041 gcgatgtatt cgcagacttt caggaacgcg tcttttggcg taccacagat gttttcaacc
     5101 acatctggcg tgtagcggga aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt
     5161 tgcagcgtgg tatcgcgttt ggcgaagccg ttttcgtcca gttcgtaagt ccaggaggat
     5221 ttatcgtact tgcgtttttc cgcgtcatag ccggtgaaaa ggccatcttc aaagccgtaa
     5281 tcctcacgca cgatcaggct ggcgttggta taggcttcgg tgtattcgcg gttgaatttt
     5341 tcattgttca gcaggtacag caatacgcct gacaggaaag caatgtcagt accggaacga
     5401 ataggggcat agtagttagc taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc
     5461 agcttcgcgc cgttgtgaat tttggcttcc atcgcccagc ggaacccgac cgggtgagct
     5521 tcagcggcgt taccgcccat caccacgacg aggttggcgt tcttgatgtc gacccagtgg
     5581 ttggtcatcg caccgcgacc aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag
     5641 acacgcgcct ggttgtcgac cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa
     5701 tagccggttt cgttactcga cgcggaagca cacagcatcc cggtggagag ccagcggtta
     5761 acagtcacgc cttcggcgtt ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt
     5821 ttggcgatgc gatcaaacgc ctcttcccaa ctgatttgct gccatttatc agaacctggc
     5881 gcacggtatt ccggaaactt cagacggctt tcggagtgga tgaaatccac caggccagcg
     5941 cctttcggac aaagtgcacc gcggttgacc gggtgatccg gatcgccttc gatatggaag
     6001 atagatgctt tggcgttttt tgctccgtca ccgaggctgt acatcaacag cccacagcct
     6061 acggaacaat aggtgcaggt attacgggtt tcgcgggtgc gcagcagttt atactgccgg
     6121 gtttccgcga gtgctacgct gggtgcaaaa cctagtgccg ctgccgtggt gcctgccata
     6181 ccgccagcgc agatcttaaa gaactgcctt ctgctgacct gcatggattg ctccttgttt
     6241 cgacattgtc acgtcccatt tacattcgct tgctgcgtgt gcagggagtg ggagttattt
     6301 ttctttgcgg aaggggccgc aaaggtccag aattggctcg atttccctcc atccaggaag
     6361 ggtttgtaac agaataccat aatgttggtg tgtgtgttct tatctggtta agagaaagtg
     6421 aaaaaaacac agcgaaaaga aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt
     6481 gagttatggc ggcgtgatga tttacaacac ccacggttag atgaggtcgc ggaagaagtt
     6541 cccgttgcgc tggtctacaa cggcatttcg catgtggtga tgatggcgtc gcccaaagac
     6601 cttgagtact ttgcgctcgg tttttcgctt tccgagggga ttatcgaaag tccgcgcgat
     6661 atcttcggca tggatgtcgt tccttcctgt aatggtcttg aagtacaaat tgagctttcc
     6721 agccgccgct ttatggggtt gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt
     6781 ggcgtatgcg gcgtggagca acttaatgac atcggaaaac cggtgcagcc gctaccgttc
     6841 acccagacgt ttgatctcaa caaactggat gatgcattac gtcatctcaa cgattttcag
     6901 ccagtggggc aactgactgg ttgtactcac gccgctgcct ggatgttgcc atctggcgaa
     6961 ctggtcggcg ggcatgaaga cgtgggtcgc catgtggcgc tggacaaact gttaggccgc
     7021 cggtcacaag aaggggaaag ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt
     7081 tatgaaatgg tgcaaaagtc ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc
     7141 gcgaccacgc ttgctgtaga agtggctgag cgctgtaatc tgacactggt aggtttttgt
     7201 aaaccgggtc gggcaacggt ttatacccat ccgcagcgtt tgagcaatta attaaaataa
     7261 gtcctgcgat atatattaaa ttgcaggact tattcatttc gtgaatttta ttatttttat
     7321 ttataaataa aacgcgtgta gtggcgtttt cttttttcat ttattatgtt cactctctta
     7381 tctatatatt tataaggcaa ttaaatgaaa aggaatttat tatcttccgc tattatagtc
     7441 gccatcatgg ccctcagtct gacgggttgt gatgataaaa aagccgaaac agaaacgccc
     7501 ccgcctgcca atagtcaacc tgccgcacca gctcctgaag ctcccgttgc aaaagcagaa
     7561 gctaaacctg aaacacctgc gcaaccggtg gtcgatgaac aagcggtttt cgacgaaaaa
     7621 atggatgtct atatcaagtg ctataacaag ttacagatcc ctgtacagcg cagtctggcg
     7681 cgttatgttg actggctgaa agattttaaa caggggccta ccggtgaaga gcgtactgtt
     7741 tatggcatct acggcattag tgaatccaac ctcgctgagt gtgaaaaagg cgtaaaaagt
     7801 gctgtggcgt taacgcctgc gctgcaacca attgatggcg tggcggtgag ttatatcgat
     7861 gctgccgtgg cgttgggtaa taccattaac gaaatggata aatattacac ccaagaaaat
     7921 tataaagacg atgcgtttgc gaaagggaaa acgctgcacc agacattctt aaaaaatctg
     7981 gaagcctttg aacctgtagc agaatcttat catgcggcga ttcaggaaat taacgataag
     8041 cgccagcttg cggaactgaa aaatattgaa gaaagagaag ggaaaatatt ccactactat
     8101 tctctggcag tcatgatttc agcgaaacaa attaataacc tgatatcgca agagaagttt
     8161 gatgttgatg cggcaatgaa gaaagtgtct gaactggaaa cgctggtggc gcaggccaaa
     8221 gaagcggata aaggcggcat gaatttctcg tttattaatt cggcaggcca gtatcaactc
     8281 gaggctaaaa aatacgttcg ccgcatcaga gataaagtcc cgtac
//
LOCUS       DAJTYD010000164         8297 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTYD010000164 DAJTYD010000000
VERSION     DAJTYD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.164"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..770
                     /gene="ppsR"
                     /locus_tag="OO585_003319"
     CDS             <1..770
                     /gene="ppsR"
                     /locus_tag="OO585_003319"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6630800.1"
                     /translation="LGHAVMSQFPVTISSITLPFVENESRARAVKDQIDAIYHQTGVR
                     PLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQEMKLDPTPIAHRTHGLNPNNLN
                     KYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSRCGKTPTSLYLAMQFGIRTANYP
                     FIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIREERRENSRYASLRQCRMEVAEV
                     EALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRMY"
     gene            926..1972
                     /gene="aroH"
                     /locus_tag="OO585_003320"
     CDS             926..1972
                     /gene="aroH"
                     /locus_tag="OO585_003320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6630801.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARTSHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2104..2295
                     /gene="hemP"
                     /locus_tag="OO585_003321"
     CDS             2104..2295
                     /gene="hemP"
                     /locus_tag="OO585_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6630802.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2299..3735)
                     /locus_tag="OO585_003322"
     CDS             complement(2299..3735)
                     /locus_tag="OO585_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6630803.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(3798..4511)
                     /gene="ydiV"
                     /locus_tag="OO585_003323"
                     /pseudo
     CDS             complement(3798..4511)
                     /gene="ydiV"
                     /locus_tag="OO585_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            complement(4757..5221)
                     /locus_tag="OO585_003324"
     CDS             complement(4757..5221)
                     /locus_tag="OO585_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6630804.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5299..6048)
                     /gene="btuD"
                     /locus_tag="OO585_003325"
     CDS             complement(5299..6048)
                     /gene="btuD"
                     /locus_tag="OO585_003325"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6630805.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6048..6599)
                     /gene="btuE"
                     /locus_tag="OO585_003326"
     CDS             complement(6048..6599)
                     /gene="btuE"
                     /locus_tag="OO585_003326"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6630806.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6662..7642)
                     /gene="btuC"
                     /locus_tag="OO585_003327"
     CDS             complement(6662..7642)
                     /gene="btuC"
                     /locus_tag="OO585_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6630807.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(7851..8180)
                     /locus_tag="OO585_003328"
     CDS             complement(7851..8180)
                     /locus_tag="OO585_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630808.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
BASE COUNT         2167 a         2214 c         1919 g         1997 t
ORIGIN      
        1 tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt acgctgccgt
       61 ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca atttatcacc
      121 agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt cgcgccatca
      181 tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc ccgctacaac
      241 aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt aaccctaata
      301 atctcaataa atatgatgcg cgcattgcgg cgattgatta cacccttgcc cacgatgacg
      361 gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt tctcgctgtg
      421 gtaaaacccc caccagtctg tatctggcaa tgcaatttgg tatccgcacg gcaaactacc
      481 cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa ccgcttcagc
      541 ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc gaggaacgtc
      601 gggagaacag tcgctatgcc tcgcttcgtc agtgcagaat ggaagtcgcg gaagtggaag
      661 ccttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg gtagaagaga
      721 ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag agaactagtg
      781 cgctggtttg ttttttgtta tcatgctaac cacccggcga ggtgtgacac acctcgcact
      841 tgaaatcagc tgcgattggt ttatcgtgat gcgcatcact tcccggtagt cctgccgtag
      901 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
      961 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgtcgc
     1021 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctgaatggtg aagataagcg
     1081 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     1141 cacccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     1201 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaaggg ctaatctccg atccggattt
     1261 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     1321 aaatgagctg ggtgtcccta ccgcgactga gtttctcgat atggtgaccg gtcagtttat
     1381 tgctgattta atcagttggg gcgcgattgg cgctcgtact accgaaagtc agatccaccg
     1441 cgaaatggct tcagcactct cctgtccggt aggttttaaa aatggtaccg atggcaacac
     1501 gcggattgcc gtggatgcta tccgcgcagc ccgcaccagc catatgttcc tctcgccaga
     1561 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     1621 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     1681 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     1741 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     1801 tacggcgatt gctggaatta tggcggaaag tttcctgcgc gaaggaacgc aaaaaatcgt
     1861 cggcggtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     1921 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     1981 ccatgcctga tgggatgggc atttctgcgc aacttgttgt cttctcaaca cattactgct
     2041 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatt
     2101 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggatgccaa tcacaagacc
     2161 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     2221 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     2281 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     2341 tggacgactg acatagtcat catctctgtc gctgaaagga tttcgcaacg cctcatgcag
     2401 gcggtgcaat tccatcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     2461 gttgcgcaat accagagctg ggttaacgct ttgtatcagt tgctgacgct cactatcgct
     2521 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagtcatcaa atgccgcacg
     2581 atcaataaac tcatcacgta gcggtgacgc cgcgctgtgc tgctcggtca gactcagcat
     2641 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     2701 tagcgcgtta tcctcttttt gctccgtcat gaagcccagt ttctgccgca tccgttgtcc
     2761 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     2821 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     2881 attatcaaag ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     2941 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     3001 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     3061 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     3121 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     3181 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     3241 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     3301 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     3361 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     3421 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     3481 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     3541 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     3601 cgatggaata ctcagcgtgt tagccagttc gatattatgc caaatcagcc gggcattatt
     3661 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     3721 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     3781 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     3841 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     3901 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctagatc
     3961 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     4021 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     4081 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     4141 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     4201 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     4261 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     4321 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     4381 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     4441 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     4501 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     4561 taaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     4621 ctttttcagg ggtctttttt tagattatcc tgattataaa cgaataatcc atttacggaa
     4681 tttttgtctg caaaatacta ctgtattcag gagtaaatgg acggcgatgt atcatcgccg
     4741 tccatttttc gacatactag attcgtctcg cctgccagaa atttttctgc caatagacat
     4801 tatcaagtga ggaacgcatc actcccttgc tggtagaggc gtggataaat tggttgttgg
     4861 tatcgtaaat acctacatgc aaaccatttt gtccggaccc cgttttgaaa aagaccaggt
     4921 cacctggcag caacttgtct ttatcaattt gcgtgccaat agaggcttgt tgtttggttt
     4981 cacggggcag ctgcaaatcg aaacgatcgc gcatcgtcac aaccacaaat cccgaacagt
     5041 ccacaccgcg ccgcgtcatg ccaccataac gatacggcgt gccatgccag ctttgtagct
     5101 ggtcgttcaa accggcaata acggtaatcg aatcagaaag tctggcattt ggcggcggtg
     5161 ctttatggtg gctacacccg gccagaaaca gtgctgtgat caaaataagg cagaaacgca
     5221 ttccgtacgg ttcctctgtt ttttattctt gcattaattt agcgtcgtaa ttacccgatt
     5281 ttcaagatac taatgaaatc agatggtcaa aatcagcatt ctgtgacctt cgatatccag
     5341 acggcgaaaa ttcatcccat aggcctgcgc cagatttggc ggcgtgagca cctcttctct
     5401 gcgtccactg gccagcattt ttccaccttt tagcaaccac gcccgatgcg catgacgcaa
     5461 cgtgtggttg agatcgtgac tgctcatcac aatcgccagt ccttgctgac acagcgcgct
     5521 cagaattttg tctaacgcac tttgttgcgc aacatcaaga ctgttcatcg gctcatcaag
     5581 aagcagcaat tggcctgcgg gattggcttg tggtgtgatt tgcaacacca ccgcagcaag
     5641 acgtacgcgt tgccattcac cgccggaaag ttgattggtg ctacgaccga gtttgtcatc
     5701 aagagccagc gcccctgcga catcattcag tagttcggta cgcgttttat cgtgctgatg
     5761 cagtgtcagg tagtgccaga ccggcattgc aaacggcggc gtctgctgtt gtgaaagata
     5821 ggcgcgatgc agcgcgagtt ttgttgcgga ccatgcttcc aggggttgcc ccgcgaactg
     5881 aatgcttccc ttaccgctgg tcattccggc cattcgtgca agcaaggtac tcttacccgc
     5941 gccattcggc cccaccaggt gcaggatctc cccagcccga acctcgccag aaagtggccc
     6001 caggcgggta gattccgcaa catcctgtaa ctgcatcaca atagacatta ttttgccaac
     6061 gccagtttaa tgctttccat cacaatgggg tcttccggcg tcatatccgg ggaaaaacgc
     6121 tggatgacca atccgtccct gccaactaaa aatttctcaa aattccataa aatatcatca
     6181 gggtacagcg gtgcacgacc tttgctgacc atacgggcat agaatccgct ctcttccggc
     6241 gcgactgcgg tcggcgctgc ggcaatcaat ttttgataca gcggatggcg tccttcgccg
     6301 ttaacttcaa tcttactgaa catcgggaac gtcacccccc atgtggtggt acagtaagtt
     6361 ttaatctctt catcgctgcc cggttcttgt tccagaaact ggttgcacgg gaatcccagc
     6421 accacaaaac ctcgatcggc ccaggctttc tgaatattct ccaactgctc atattgcggc
     6481 gttaagccac actttgaggc gacattgaca atcaacagca cattaccggc gtacttctcc
     6541 agcgtggtca cttcaccgtc gatatctttc actacggtcg tcagaatgga atcttgcatc
     6601 gtttctcctg ggtgtggtca gtaaaaatct tagcttttaa tcatagaccg tctttttgcg
     6661 gctaacgtct tgcttttaac aataaccaga taaacaccgg cgcacccaac gttgcggtga
     6721 ccacgccaat aagcagctct gcggcagcta atgccaggcg cgctacaata tcggccagca
     6781 gcaatgcgct cgcccctgcc agcgcgcagc cgggaagtaa tacgcgatga tcggttaaac
     6841 cacacaaccg aagaatatgt gggatcacca gaccaataaa gccgatagca cccgccagcg
     6901 ccacactgac gccaaccatc cagccggtcg cggccaccag cacattgcgc cagaaccaca
     6961 ggggtaaacc cagttgccgc gccgagatct cgccaagtgc taacatattc atcggcctgg
     7021 actgacaaca gatccacaac aacacgggga tcaatgccag catcagccag ctttgccgcc
     7081 agtctacgcc gccaaaaccg cccatcatcc agtacatcag ctgacgtaaa tcaacagagg
     7141 tggaaaagta gatagcccac gtcattagtg cgctacagat aatccctaat gcaacgccag
     7201 ccagcaataa ccgactggtc gaaagatgac gacgggcgaa acgtaagagt attaaagtaa
     7261 tgataagcgc gccagcaatc gcacacagcc ctagcgccca gttggggagt tgcccttgcc
     7321 caagcaatac cgcggcgata agccccacgc ctgcgccgtt agagacgcca agtagtccag
     7381 gttctgccag aggattttca aacaacgcct gcattacagc gccggatata gccagcgccg
     7441 caccaaccaa caatacagcc agcgtacgtg gcaggcgaat ttgccagacg aacagttcgc
     7501 cacgaggagt aaaccagtca cctagcgaga tccattgttc accggcgcaa aggcttaaaa
     7561 gaagcgccag cagcatcaaa actgacaggc ataataacca gcgaatattt tgtcgctgtt
     7621 gttggcgggc aagtgtcagc atggtatccg ttctgctgaa gtgtcatggc gttgatttta
     7681 cggtgactct tcgacagtga aaagaaaaaa ggccgcagag cggccataaa cacaagtaaa
     7741 aatcaacaag ttagataatt atcaaagact tacagacaca caaaaacaca gccaaccaca
     7801 acaaataaca gggatgtggt cactttgtgg atcatatcgc cgccataaat ttacttaata
     7861 acagtaacca cagccacaag cacggccatc gcagacagga caatacctgt gagtaaccaa
     7921 gtctgatttg caaatgattt ctgaagttca gtacgatgct cagccatctc aactttcaga
     7981 gactggcgta aatcagccat ctcagacttc agggattggc gcgattctgc catctcaagc
     8041 ctcaaccctt cgcgaatctc cagtacatca gatttggtag caaaggattc gcttcttgtg
     8101 gtgagagtaa tcaggttgtt tttgatttca gtcacatcac tttccaggca actgacccgc
     8161 cgttcaaggt cgtcattcat gccatcacct ccatcattac cacttcctgt tacttggttg
     8221 ttggatacat tataggcatt ctctttcttt tttctgaacc gcgaaatatg tgcaacattc
     8281 tctgccatca cttctca
//
LOCUS       DAJTYD010000165         8184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTYD010000165 DAJTYD010000000
VERSION     DAJTYD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.165"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(313..891)
                     /gene="mocA"
                     /locus_tag="OO585_003329"
     CDS             complement(313..891)
                     /gene="mocA"
                     /locus_tag="OO585_003329"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6630809.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            994..1764
                     /gene="yqeC"
                     /locus_tag="OO585_003330"
     CDS             994..1764
                     /gene="yqeC"
                     /locus_tag="OO585_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6630810.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1812..3437
                     /locus_tag="OO585_003331"
     CDS             1812..3437
                     /locus_tag="OO585_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6630811.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHINRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OO585_003332"
                     /pseudo
     CDS             complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OO585_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OO585_003333"
     CDS             complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OO585_003333"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCR6630812.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5896..7107)
                     /locus_tag="OO585_003334"
     CDS             complement(5896..7107)
                     /locus_tag="OO585_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6630813.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7165..>8184)
                     /gene="dpaL"
                     /locus_tag="OO585_003335"
     CDS             complement(7165..>8184)
                     /gene="dpaL"
                     /locus_tag="OO585_003335"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6630814.1"
                     /translation="KKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSFE
                     HLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAEC
                     IVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMRE
                     MGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIVN
                     VGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRII
                     SGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEGK
                     HAVAP"
BASE COUNT         1934 a         1992 c         2134 g         2124 t
ORIGIN      
        1 cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga
       61 tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc
      121 ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga gaattaaaat
      181 cactcctgaa aattattatt gccagcccat atatcacttt ttctcttttc tcaaaatcag
      241 tgattcctta tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt
      301 taactacgtt ttttaaattt tagtatacct tttttgcact gtaataaagt cgtccggagt
      361 atcaatatct aaaattattt ctgcattttc tacttcgacg gaataatgag ctcccataag
      421 gagtgcctga cgcatattgg tgatattggg gcgcttgatt gcctgcatca ggcatggttt
      481 tgacactaaa atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt
      541 tcgtaacgac cagatttttc tgaaaatatc gatggtgagg gttggcatgt caccgtgggt
      601 gagaaaacaa tgttctgttt gcactgcggg tactgcggcc tttactgatg ttagtaaacc
      661 ctgcgcataa tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg
      721 cagttcatta ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt
      781 tttgatactt gtatcaagaa ttgtcccctg ttgccagggt aacatcattt tccattgccc
      841 cattcttgat gataatccgg cagcggtaat tatacagtcg atggctgaca tagtggttcc
      901 attatttata ggggctaata acgcctgata attgcgttac ttcctgatgg attaagttgg
      961 ggataattta tcccagagag gtcataaaga ctcgtgaaaa gtatagttga cccatcggca
     1021 ttattcattg atttaggtgc gcagaaacgt cccactgtga tttctgttgt tggtgctggg
     1081 gggaaaacca gcttgctttt ttggctggca gaactgctcc aggctagcgg caggcgtgtg
     1141 ttaatcacga cgacaacaca tatgtttatg ccaacgtctc actggcccgt ggttttctgt
     1201 cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc ccatttcatt ttgttttcac
     1261 agctggaaag cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat tgatgcactg
     1321 gtgcaacgac cagagtgtga cgtaattctc attgaagcag atggctcgcg tgcaatgccg
     1381 ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa gcagttgctg cgtgattgct
     1441 gtgatgggag gacatatttt gggcgcgaaa gtgagcacag aaaatgtcca tcgctggtcg
     1501 cagtttgctg atattactgg gttaacacct gatgcaacct tgcaactgag cgatctcgtt
     1561 gcgctggttc gccaccctca gggggcgttt aaaaacgtac cgcaaggttg tcggcgagtc
     1621 tggttcatta accgtttttc tcaatgtgag aatgcgattg cacaaagcga gctccttcaa
     1681 ccgctgcaac aacacgacgt agaggcaatc tggctaggcg atatacaaga acatcctgca
     1741 atcgcgcgca gatttgtgaa ttagcggccc gggtattccg ggttactgaa aggatatcca
     1801 ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa aattgtccgt
     1861 ttgcgatggc gcaaattgtt gatagccgag gctcgactcc ccgccattct gcacaaatgt
     1921 tagtgcgcgc cgatggttct atcgtcggta caattggtgg cggaatggtt gagcggaagg
     1981 tgattgaaga gtcacttcag gcattgcagg aacgtaagcc gcgattattc catggacgta
     2041 tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg tcagtattta
     2101 tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat atcaaccggg
     2161 cgatagccca gagtgccgcg ctattaggat ttgatattgc cgttgccgat atttatcgcg
     2221 aaagcctcaa tcctgaacta ttcccaccat caaccacgct tctccatgct gagtcgtttg
     2281 gtgcggcagt ggaagcactg gatattcgcc ctgataattt tgtcctgatt gccacgaata
     2341 atcaggatcg tgaagccctc gacaaactca ttgaacagcc cattgcatgg ttggggttgc
     2401 tggcaagtcg ccgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa ggcgtggctg
     2461 aagaacatat tgcccgttta catgcgcccg ttggttacaa cataggtgcg gaaacgccgc
     2521 aggagatcgc catcagcgtg ctggcagaaa tattacaggt gaaaaataac gcgccgggtg
     2581 ggctgatgat gaaaccttct catccttccg gacaccagct ggtggtgatt cgcggtgcgg
     2641 gagatatcgc cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa gtgatcatgc
     2701 tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag gccgtgttcg
     2761 atggcgaaat gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct gcggaagcga
     2821 tgaaacttac cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt tcattgcttg
     2881 atgaactgaa accgctttgc gtggtggacg ctattctggc gaaacagaat ttgggaacgc
     2941 gggcagatat ggcaccagta acaatcgcgc ttgggccggg ctttactgca gggaaggatt
     3001 gtcatgcggt aattgaaaca aatcgcgggc actggctcgg tcaggtgatt tactctggtt
     3061 gtgcgcagga gaataccggt gttcctggca atattatggg gcataccacc cgacgggtga
     3121 tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa attaggcgat ctggtgaaag
     3181 agggcgatgt gattgcctgg attggtgagc atgaaattaa agcaccgttg acggggatgg
     3241 tgcgtggctt gttgaacgac ggcctggcag tggttggtgg ttttaaaatt ggtgatatcg
     3301 atcctcgtgg tgaaaccgct gatttcacca gcgtttctga taaagcccgg gcgattggcg
     3361 gcggcgtact tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg acaaaagaag
     3421 tgctggaagt ggcttaaatg aaaatggccg gatggtatta cccacccggc cttaaaatta
     3481 ctgacaaatc accgttccgg ttttgccttc aatcccttct ttcgctttgc tcaatacggt
     3541 aatcagtgct tcacgacctg cgcgagagcg agcaaacgag gcggcagctt ccactttagg
     3601 cagcatagaa cctttcgcaa aatgaccttc ttcgataaag cgttcagcat cacttaatga
     3661 caggcgatcg agccactgtt cattctcttt accgaagtta atggccactt tttctaccgc
     3721 agtcagaatg atcagcatat cggcatcaat catttctgct aaacgggcgc tggcccagtc
     3781 tttatcgata accgcgctgg caccacgcag atggttacct tcacgaataa cagggatacc
     3841 accaccgcca acggtgatca ccacctggcc tgcatctacc agagctttaa cagtttcttt
     3901 ttcaatgata tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc gaccagcatc
     3961 ttctttcaga gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg taaagaacga
     4021 accgatcggc ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt cgacctgagt
     4081 aaccagcgtt gctacaggtt tattaatgcc ccgagaaagc agttcttctc ttaacacgtt
     4141 ttgtagatcg taaccaatat aaccctagct taaggcaaca cagacagaca tcggcagcat
     4201 tggcgagtgt gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca tgcccacctg
     4261 cggcccgtta ccatgagtga cgataacttc atgtccttgg gcaattaaat caacaattgc
     4321 ctgagaagta atttttaccg ctttcatttg tccggccagg tcatcgccca gcgcattccc
     4381 gcccagggcg agaacaattt tcttactcat ttttcctcac tcatcattct tgattgtgta
     4441 tagatccctg acagtggtta gagcacggga gggacaaacg gtttgcgtcg caggaaacga
     4501 cctcgcccgg ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc acgggataag
     4561 gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc ggcgttgtcg
     4621 tggagatggc gatgctgaat ttgttggctc tgacgtgggt cgataatcac cacgtcgcca
     4681 tcggaaccgg gcgctaataa tcctttttgc ggccacaggc caaacagcct ggcgggcatt
     4741 gcgctggtta attcaacaaa gcgttccggt gttatacgtc ccgtcatcac gccactggaa
     4801 aacagtaact gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg actgaaatcg
     4861 cctttagaaa tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt cgccaccacg
     4921 tcaatcgcac catcgctgat gccacaccac agtttgtcct gctcgcgtac gttacgcagc
     4981 ggtgggctaa gaatgaactt catgccatct tctgtatcgt aactgcgttc gtccaacagg
     5041 agatattgtg ggcaggtttc aacccagact ggctggtgat tcgcacgggc aagacgcaga
     5101 taatccagac ctaagccgtt agacaggtgc acgatataga gcggggcgtt accggcaatt
     5161 tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc attccagagg gcgactcaag
     5221 gcatgatagc gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt gctggcgata
     5281 gccgcatcat tttccgggtg cacggtggtc agcgcgccgg attcatgcaa gcggcgtaat
     5341 gcctgcaaaa cctcgtcatc gttgagtttg tattgatagg ttaaatagag tttaaaactg
     5401 ctcagtcctt cctcgaccat catcgggatt tcgtcgagga ttgcgtgatt aatgtgctgg
     5461 atcacaccgt gaaagctgta atcgatgacc gctttatggg cggcataacc acgataaacc
     5521 tccagttgat ggcgtaaccg acagccgttt gggccaaatc ccatatggtc aataatggtt
     5581 gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca actgcgcgcg
     5641 atgccgacat caatattgaa atgcgtatgg acatccacgc cgcctgggaa aacgtaacag
     5701 ccagtggcat caatttcttc atacgggagc tgcggcgaaa tattgttgcc caactggcga
     5761 acaatcccgc tttcaatcag caaatcctgt ttggcttgtc catctgcgtt aacgacagtg
     5821 ccgtttttga tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa ccggagggga
     5881 ataagtgaag agtaattact ctgttgccag ccagcttaac gggattgcag cgtacatcgc
     5941 ggcacaggtc accaggtgag atttccaggt tttttcgtta ggtgcatgcg cttcaggttc
     6001 tttacccggg ccaaagccga tcaccggaat gccgtgacgg cccatgatag atacgccgtt
     6061 agttgagaag gtccacttat caacaaccgg cgctttgcca aacagccctt cgtaggcatt
     6121 caccagtgct ttaacggtga agtgatcttc ttccactttc caggtcggga agtagcattc
     6181 ggttgggtaa accaggccag tccaggacgg acggtcgtag ttgtacatag aaacaaccgc
     6241 gttagctttc tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt cccaggtttc
     6301 gccccaggtc agacggcggt caatagagac ggcgcagcta tctgctacag cgcaacggct
     6361 tggggatgtg aagaagattt cagaaacggt gagggtgcct ttgccgagga attcgtcata
     6421 acccagacgt tgggagagtt cttgtaattc gccaagaatc ggacccattt tgaaaatggc
     6481 gttgtcaccg cgttctggcg cagaaccgtg gcagctaaca ccctgaacat caatacgaat
     6541 ttccatacga ccgcgctgac cacggtatat ctggcagtcg gttggttcgg tactgaccac
     6601 aaattccggg cgaatgccgg attgttcaat aatgtactgc cagcacagac cgtcgcagtc
     6661 ttcttcctgc acagtaccgg taaccagcag ggtatattca tcttccagac cgaggtcttt
     6721 aatgatttta ccggcataaa ccatagatgc catgccgcct tcctggtcgg aagtaccgcg
     6781 accaccgatt agctcatcag tttccatgcc ttcgtacgga tcgaagtccc agtttttgat
     6841 gttgccaatg ccgacggtat cgatatgagc gtccattgcc accagacgcg ggccgtggcc
     6901 gatataaccg agaacgttgc ccatcgggtc gatttcaact ttatcgaagc cgactttttc
     6961 catctcttct ttaatacgat gtactacgcg tttttcgtcg cagctttcac tgggaatagc
     7021 aaccatgtcg cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt tttcaagaat
     7081 cagtttgaat ggaatattct tagccattat ttttctccag attttttact ccccgttttg
     7141 cagggagtag ttccaatagg gtgattaagg tgctacagcg tgtttgcctt cccagacaac
     7201 ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca ctacggcatc
     7261 tttgttcagc gccagttttt ccatcaggct ttgacgttgc gggtgataat gaaccgctgc
     7321 gagaacgccc aaaccgacag cgccggattc accggagatg atgcgcgggt cgttgccgta
     7381 cggattaccc agcacgcgca tacctaatgc ggcaacgctg tcctggcagg agatgaattg
     7441 ggtggcgcag ttacgtagga tttcccagcc cagcgggtta ggttcgccac aggccaggcc
     7501 cgccatgatg gtggccatat caccgccaac gttgacgatg tcgcctttga cgccggagcg
     7561 ataaatacag tcagctttgt caggttcaac aataatgctg tgcagatttt gcgggctgta
     7621 gacgtcgacc agataaccca gcacaccacc ggccattgct ccgacaccgg cttgcagcag
     7681 aacgtgcgtc ggggttacgc ccatttcacg catttgctcg acggcttcat ctgccagggt
     7741 tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa ccttcccatg ccgtgtcctg
     7801 taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg tatcgtcata
     7861 gttcatatcc gtgacgatgc actcggcacc gaggttcaga atggcgtcaa cgcgttcctg
     7921 agcagaacct ttcggcatgt aaatcaccgc attctgtccg agttgctgtg ctgcccacgc
     7981 cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt cgccgatggc
     8041 atttttcagg tgctcaaatg acagcgtttc gatatcaaga tgatattttt cacacaataa
     8101 ttgagcgatg gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga atcgttttga
     8161 ttcgtctttg acgagaattt tctt
//
LOCUS       DAJTYD010000166         8168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTYD010000166 DAJTYD010000000
VERSION     DAJTYD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.166"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..659)
                     /locus_tag="OO585_003336"
     CDS             complement(<1..659)
                     /locus_tag="OO585_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR6630815.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     T"
     gene            887..1495
                     /locus_tag="OO585_003337"
     CDS             887..1495
                     /locus_tag="OO585_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6630816.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1593..2984
                     /gene="selA"
                     /locus_tag="OO585_003338"
     CDS             1593..2984
                     /gene="selA"
                     /locus_tag="OO585_003338"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6630817.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2981..4825
                     /gene="selB"
                     /locus_tag="OO585_003339"
     CDS             2981..4825
                     /gene="selB"
                     /locus_tag="OO585_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6630818.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            5015..6166
                     /gene="yiaY"
                     /locus_tag="OO585_003340"
     CDS             5015..6166
                     /gene="yiaY"
                     /locus_tag="OO585_003340"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6630819.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            6331..7869
                     /gene="aldB"
                     /locus_tag="OO585_003341"
     CDS             6331..7869
                     /gene="aldB"
                     /locus_tag="OO585_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6630820.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT         1904 a         2087 c         2299 g         1878 t
ORIGIN      
        1 tgtgtgcgcc cgaagcggtc caccagcccg gtcagtaccc ggtacggcgg cagcggcgca
       61 ggcagcacct catccgcttc cggcacccgc tcacaccagc cgagcagcca ccacggcccc
      121 tgcggactgt ttgtcgccag ataacgatgc ggacttaagc ggagttcttc cggcagcgcc
      181 tgccagagtg cggccagccg gtgaccttca tccagtttcg ccacgccgcc gcgcaccagc
      241 cacagtgact cgctgcggct gtaaccgtcc tcaccgggaa acaggtgctc aaaatacagg
      301 cttctgccgc cgttatcact gagtatcagt gtgttatcgc gcagctgtaa gcggatatcc
      361 gcaggcattt tccagccggg gccgaggctc cccaccggcg cgggcgtttt tgtccggtaa
      421 ctgctgtagg tgcgggagag gatgaacggc agcgggccgg gcagggcgat gtcggtttca
      481 ccgggaagga cctttgcacc gagcagggga ttgaccggat ggccggacgt cactccgccg
      541 gggcacaccg aacaggccac gccggtgggg gcaccaatgc gcaccccggc tgaaccctga
      601 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatt
      661 tctcatcctt gttttatcgg gttatttgtc tgctgacagg caaaacccta cagatttata
      721 agaaagcctg aagtgagata tccatcagag tgaaataatt aggaaaatat ttatatcacc
      781 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      841 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      901 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      961 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc
     1021 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat
     1081 catcgctgaa tatattgagt taatgaatgt cgctccggcg atgttgccgc gcgatccgct
     1141 ggagtcgttg cgggtgcgca aaattgaggc gctggcggat ggcattatgg atgccgggct
     1201 ggtatcggtg cgtgaacagg cgcgtccggc ggcgcagcag tctgaagatg aattgttacg
     1261 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
     1321 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgctg tcggatatct
     1381 caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt
     1441 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
     1501 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
     1561 atgtgagagg gccttgatta gccaggtttc ctatgacaac cgaaacgcgt tccctctata
     1621 gtcaacttcc ggctattgat cgcttattgc acgatagctc cttcctttct ttgcgtgata
     1681 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1741 tgattcgtga cagccagacg cttcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1801 cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1861 tgctgcatac caaccttgga cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1921 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgctgga cgcggacatc
     1981 gcgatcgggc gctggcgcaa ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg
     2041 tcaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     2101 tggtagtatc tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     2161 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     2221 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     2281 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     2341 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     2401 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     2461 gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag
     2521 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     2581 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     2641 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2701 gtttacaggc tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2761 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2821 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2881 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2941 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     3001 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     3061 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     3121 gccggatggt cgcgtgccta gttttatcga cgtacccggt catgaaaagt ttctttccaa
     3181 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     3241 cgtgatggcg cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     3301 gacagtggcg ctgaccaagg ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg
     3361 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     3421 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccaggacg
     3481 tgaacacgcc agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg
     3541 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     3601 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc
     3661 aacagaaacc gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3721 agagcagatt aaccgtggcg actggctgct tgccgatgcg ccgccagagc cgttcacacg
     3781 ggtgattgtc gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca
     3841 ccacgccgcc agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact
     3901 ggtcttcgac accccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc
     3961 tgcccgcaac acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa
     4021 acgtaaacca gagtatctgc aatggctggc ttccctcgcc agggcacaga atgatagcga
     4081 agcgttagcc gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg
     4141 ccagctcaac ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg
     4201 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     4261 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     4321 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     4381 aagcggcgac atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt
     4441 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttagtg acgagccgtg
     4501 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcttgacgct
     4561 aagccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     4621 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4681 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4741 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga
     4801 cgcattatta tttccggaaa aataaggaaa tgattaatta atttttaaaa taaattaata
     4861 caaatttctt atgattttaa aaaaagcaca ttgtttaatg aatacaatgt gctttttatt
     4921 agattaattt tgataattaa taataatgat catgttttcg aatgaaaatc ctcagtaagc
     4981 ttcccaccct tttttacact ttcaggagtg tgttatggca tcttcaactt tctttattcc
     5041 ttctgtgaat gtcatcggcg ctgattcatt gactgatgca atgaatatga tggcagatta
     5101 tggatttacc cgtaccttaa ttgtcactga cagtatgtta acgaaattag gtatggcggg
     5161 cgatgtgcaa aaagcactgg aagaacgcaa tatttttagc gttatttatg atggcaccca
     5221 acctaacccc accacggaaa acgtcgccgc aggtttgaaa ttacttaaag agaataattg
     5281 cgatagcgtg atctccttag gcggtggttc tccacacgac tgcgcaaaag gtattgcgct
     5341 ggtggcagcc aatggcggcg atattcgcga ttacgaaggc gttgaccgct ctgcaaaacc
     5401 gcagctgccg atgatcgcca tcaacaccac tgcgggtacg gcctctgaaa tgacccgttt
     5461 ctgcatcatc actgacgaag cgcgtcatat caaaatggcg attgttgata aacatgtcac
     5521 tccgctgctt tctgtcaatg actcctctct gatgattggt atgccgaagt cactgaccgc
     5581 cgcaacgggt atggatgcct taacgcacgc tatcgaagca tatgtttcta ttgccgccac
     5641 gccgatcact gacgcttgtg cactgaaagc cgtgaccatg attgccgaaa acctgccgtt
     5701 agccgttgaa gatggcagta atgcgaaagc gcgtgaagca atggcttatg cccagttcct
     5761 tgccggtatg gcgttcaata atgcttctct gggttatgtt catgcgatgg cgtaccagct
     5821 gggcggtttc tacaacctgc cacacggtgt atgtaacgcc gttctgctgc cccatgttca
     5881 ggtattcaac agcaaagtcg ccgctgcacg tctgcgtgac tgtgccgctg caatgggcgt
     5941 gaacgtgaca ggtaaaaacg acgcggaagg tgctgaagcc tgcattaacg ccatccgtga
     6001 actggcgaag aaagtggata tcccggcagg cctacgcgac ctgaacgtga aagaagaaga
     6061 tttcgcggtt ctggcgacta atgccctgaa agatgcctgt ggttttacta acccgatcca
     6121 ggcaactcac gaagaaattg tggcgattta tcgcgcagcg atgtaatcat catttccaca
     6181 acggctggca aattgtcagc cgctttttca actatctctg taacccttgc ccgtaaattc
     6241 gtgatagccg tcgtaaagct gttaccgact ggcgaagatt tcgccagtca cgtctaccct
     6301 tgttatacct cacaccgcaa ggagacgatc atgaccaata atcccccttc agcacagatt
     6361 aagcccggcg agtatggttt ccccctcaag ttaaaagccc gctatgacaa ctttattggc
     6421 ggcgaatggg tagcccctgc cgatggtgag tattaccaga acctgacgcc ggtgactggg
     6481 cagctgctgt gcgaagtggc gtcttcgggc aaacgagaca tcgatctggc gctagatgct
     6541 gcgcacaaag tgaaagataa atgggcgcac acctcggtgc aggatcgcgc ggcgattctg
     6601 tttaagattg ccgatcgaat ggaacaaaac ctcgagctgt tagcgacagc tgaaacctgg
     6661 gataacggca aacccattcg cgaaaccagt gctgccgacg tgccgctggc gattgatcat
     6721 tttcgctatt tcgcctcgtg tattcgggcg caggaaggtg ggatcagtga agttgatagc
     6781 gaaaccgtgg cctatcattt ccatgaaccg ttaggcgtgg tggggcagat tatcccgtgg
     6841 aacttcccgc tgctgatggc gagctggaaa atggctcccg cgctggcggc gggcaactgt
     6901 gtggtgctga aacccgcacg tcttaccccg ctttctgtac tgctgctaat ggaaattgtc
     6961 ggtgatttac tgccgccggg cgtggtgaac gtggtcaatg gcgcaggtgg ggaaattggc
     7021 gaatatctgg cgacctcgaa acgcatcgcc aaagtagcgt ttaccggctc aacggaagtg
     7081 ggccaacaaa ttatgcaata cgcaacgcaa aacattattc cggtgacgct ggagttgggc
     7141 ggtaagtcgc caaatatctt ctttgctgat gtgatggatg aagaagatgc ctttttcgat
     7201 aaagcgctgg aaggctttgc actgtttgcc tttaaccagg gcgaagtttg cacctgtccg
     7261 agtcgtgctt tagtgcagga atctatctac gaacgcttta tggaacgcgc catccgccgt
     7321 gtcgaaagca ttcgtagcgg taacccgctc gacagcgtga cgcaaatggg cgcgcaggtt
     7381 tctcacgggc aactggaaac catcctcaac tacattgata tcggtaaaaa agagggcgct
     7441 gacgtgctca caggcgggcg gcgcaagctg ctggaaggtg aactgaaaga cggctactac
     7501 ctcgaaccga cgattctgtt tggtcagaac aatatgcggg tgttccagga ggagattttt
     7561 ggcccggtgc tggcggtgac caccttcaaa acgatggaag aagcgctgga gctggcgaac
     7621 gatacgcaat atggcctggg cgcgggcgtc tggagccgca acggtaatct ggcctataag
     7681 atggggcgcg gcatacaggc tgggcgcgtg tggaccaact gttatcacgc ttacccggca
     7741 catgcggcgt ttggtggcta caaacaatca ggtatcggtc gcgaaaccca caagatgatg
     7801 ctggagcatt accagcaaac caagtgcctg ctggtgagct actcggataa accgttgggg
     7861 ctgttctgat ataagaagct ggtcgcattg ggtattcatt gcctgatgtg acgcttacgc
     7921 gtcttatcat gcctacggga acctgaccgt aggccggata aggcgtacac gccgcatccg
     7981 gcataaacaa agcgcacgtt gttacaattt acgttttaaa cctacgtgcg caataatcca
     8041 gactacgcca acagaagtgc gtttctgggg cgtgttttgt caggagttta atctcgcgtt
     8101 tcgatggtga gcgggcgaac taggccgtaa atcaccattg gtgatccgag gccaatcagc
     8161 aataaacg
//
LOCUS       DAJTYD010000167         8138 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTYD010000167 DAJTYD010000000
VERSION     DAJTYD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.167"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..250
                     /locus_tag="OO585_003342"
     CDS             <1..250
                     /locus_tag="OO585_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6630821.1"
                     /translation="GMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRL
                     NGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(389..1018)
                     /locus_tag="OO585_003343"
     CDS             complement(389..1018)
                     /locus_tag="OO585_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6630822.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1141..2787)
                     /gene="fumB"
                     /locus_tag="OO585_003344"
     CDS             complement(1141..2787)
                     /gene="fumB"
                     /locus_tag="OO585_003344"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6630823.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRI
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2865..4205)
                     /gene="dcuB"
                     /locus_tag="OO585_003345"
     CDS             complement(2865..4205)
                     /gene="dcuB"
                     /locus_tag="OO585_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6630824.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4776..5495)
                     /gene="dcuR"
                     /locus_tag="OO585_003346"
     CDS             complement(4776..5495)
                     /gene="dcuR"
                     /locus_tag="OO585_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6630825.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5492..7123)
                     /gene="dcuS"
                     /locus_tag="OO585_003347"
     CDS             complement(5492..7123)
                     /gene="dcuS"
                     /locus_tag="OO585_003347"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6630826.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7304..7534
                     /locus_tag="OO585_003348"
     CDS             7304..7534
                     /locus_tag="OO585_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6630827.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7546..7818
                     /locus_tag="OO585_003349"
     CDS             7546..7818
                     /locus_tag="OO585_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6630828.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8045..>8138
                     /gene="ghoS"
                     /locus_tag="OO585_003350"
     CDS             8045..>8138
                     /gene="ghoS"
                     /locus_tag="OO585_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6630829.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLM"
BASE COUNT         2025 a         1967 c         2079 g         2067 t
ORIGIN      
        1 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
       61 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
      121 tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc tggataaata
      181 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
      241 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
      301 acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgct
      361 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
      421 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
      481 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
      541 gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt atcgccagcg
      601 ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgaacga
      661 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc agcttgtcat
      721 acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg actttcgcgt
      781 aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc gtatagagca
      841 gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca ataatcaccg
      901 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
      961 ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     1021 tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     1081 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     1141 ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt
     1201 gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     1261 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     1321 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg tgtcggtaac
     1381 ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag
     1441 atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc
     1501 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     1561 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     1621 aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat
     1681 ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag
     1741 ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg
     1801 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca
     1861 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     1921 aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac
     1981 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc
     2041 gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc
     2101 gatatggtac ggcgggcagg ctgcagtacc gaggatacgc attttctcga cgaggaagtt
     2161 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc
     2221 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     2281 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     2341 attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     2401 ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcgat
     2461 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat ttcttaagaa
     2521 ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     2581 ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt gctgcgccag
     2641 cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     2701 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     2761 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     2821 gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac
     2881 atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga
     2941 ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     3001 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     3061 gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac aatcgccgcc
     3121 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcataggcc
     3181 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     3241 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     3301 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     3361 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     3421 aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg
     3481 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag cgtcgcggta
     3541 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
     3601 ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat accgattaac
     3661 gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg
     3721 acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
     3781 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
     3841 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
     3901 atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
     3961 agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
     4021 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga
     4081 aggtggaaga caaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
     4141 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
     4201 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
     4261 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
     4321 ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact
     4381 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg
     4441 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt
     4501 tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt gtgggtgaaa
     4561 atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa
     4621 aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata attatatttg
     4681 atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg
     4741 gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt
     4801 gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg
     4861 atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca
     4921 cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg
     4981 tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga
     5041 ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa
     5101 tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag
     5161 cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta
     5221 atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta
     5281 tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc
     5341 aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct
     5401 gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc
     5461 attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc ccgtcccagg
     5521 gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc
     5581 cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg
     5641 tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat
     5701 cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac
     5761 cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca
     5821 gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg tttaaaatca
     5881 gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg
     5941 gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg gctgttttga
     6001 gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca
     6061 atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt
     6121 cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga
     6181 tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg
     6241 taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct
     6301 gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca
     6361 gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga
     6421 cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt
     6481 agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca
     6541 gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt
     6601 gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt ttatgatttt
     6661 catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga
     6721 cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac
     6781 gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca ataaacagca
     6841 gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt
     6901 tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct
     6961 tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc agatgcacca
     7021 ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta ctcaatttca
     7081 tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac
     7141 aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg
     7201 ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg
     7261 ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact
     7321 ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat
     7381 atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa
     7441 gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg
     7501 cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa
     7561 ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga aattgtcttt
     7621 gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa
     7681 gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa
     7741 cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac gcgggagtat
     7801 gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg ctctccgcgg
     7861 agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagcgcg
     7921 tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga
     7981 atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg ataggagtca
     8041 tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt atccatcatc
     8101 ttattcctca gtgttcttaa ggttaagatc attgatgt
//
LOCUS       DAJTYD010000168         8124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTYD010000168 DAJTYD010000000
VERSION     DAJTYD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.168"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..613)
                     /locus_tag="OO585_003351"
     CDS             complement(<1..613)
                     /locus_tag="OO585_003351"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6630830.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGS"
     gene            849..1121
                     /locus_tag="OO585_003352"
     CDS             849..1121
                     /locus_tag="OO585_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6630831.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(1087..1494)
                     /gene="nudG"
                     /locus_tag="OO585_003353"
     CDS             complement(1087..1494)
                     /gene="nudG"
                     /locus_tag="OO585_003353"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6630832.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            1581..2192
                     /locus_tag="OO585_003354"
     CDS             1581..2192
                     /locus_tag="OO585_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6630833.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(2201..3508)
                     /gene="ynjE"
                     /locus_tag="OO585_003355"
     CDS             complement(2201..3508)
                     /gene="ynjE"
                     /locus_tag="OO585_003355"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6630834.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(3575..4228)
                     /locus_tag="OO585_003356"
     CDS             complement(3575..4228)
                     /locus_tag="OO585_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6630835.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(4228..5763)
                     /locus_tag="OO585_003357"
     CDS             complement(4228..5763)
                     /locus_tag="OO585_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6630836.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCTLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALAAKWVGYVRQ
                     GLR"
     gene            complement(5736..6902)
                     /locus_tag="OO585_003358"
     CDS             complement(5736..6902)
                     /locus_tag="OO585_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6630837.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(6912..7460)
                     /locus_tag="OO585_003359"
     CDS             complement(6912..7460)
                     /locus_tag="OO585_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6630838.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(7460..>8124)
                     /locus_tag="OO585_003360"
     CDS             complement(7460..>8124)
                     /locus_tag="OO585_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6630839.1"
                     /translation="LIILLFAMLLAWALLPGVHEFINRSVAAFAAVDQQGIERFIQSY
                     GALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLSWTSSMAGAALCFFIA
                     RVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPFDPISYAAGLTSIRFR
                     SFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTVVIFMAKKIWLERQKR
                     ND"
BASE COUNT         1953 a         2219 c         2138 g         1814 t
ORIGIN      
        1 gactaccgcc aaatgaaagg cccttaccgg tgaagacgca ggcggtattg ttggagagct
       61 ttttcatcat ccccgccata aagccgactt cacgaccacc aacgccgata tcacctgccg
      121 gaacgtcagt atccgcgccc agatgacgat aaagctcggt catcagcgcc tggcagaaac
      181 gcatcacttc gctttcgctt ttacctttcg ggtcaaaatc gctaccgcct ttaccaccgc
      241 ccatcggcag ggtagtcagg gcatttttga aggtttgttc gaagccgagg aatttgagaa
      301 tggagaggtt aactgacgga tggaagcgca taccgccttt gtacgggccg atggctgagc
      361 tgaactgcac acgccatgca cggttgacct gtacctggtt gcgatcatca acccatacca
      421 cgcgaaactg gatcacgcgc tccggttcaa ccagacgctc cagtaatgac atctggcgat
      481 attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga acggcttgcg
      541 cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac tccagagaat
      601 atgtctgatc catagatata aaacccttat atattaatac gattgtgctt atgttgttta
      661 tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta agcaagaaaa
      721 aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat gcgataactc
      781 agacgctaaa gttttgccac ccggctaccg ttatcagttg cagagacaat aacgttaagg
      841 agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta attgcactgg
      901 ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg ttcagttcag
      961 gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa aattattctg
     1021 aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgggat gacaaaaccc
     1081 ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta atagtggaat
     1141 gtcagtaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga ccagcgcctg
     1201 atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat gaagatggat
     1261 aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag ttgcttcgat
     1321 gcccaattct tcgtttaact cacgcaccag tgcctgctgc tggctttcat ccagctcgac
     1381 tttaccaccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc gttgcgcgag
     1441 taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt tcataaatta
     1501 cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa tcagaaaggc
     1561 tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt aaaccgttgt
     1621 tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt ttaacattag
     1681 tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg tatctggcgg
     1741 cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg ctggcgcggc
     1801 gcagagagct taccgacgcg ggcggctttc ttgatatttc tctcgatttt ctcttttacg
     1861 cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg gcaggcggct
     1921 ggttgttgtt tgcgtttatc agcacgggca gcagttttct cgcctttgct gcactggcag
     1981 caaaacatca gattgataac cccggttatg cacataagtc gttttactat ctggggggat
     2041 taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc cctgcatggt
     2101 ttgcgtggat cttcggtgcg ttgtgctgga tgacgacctt tacgcgggtg tggagtgggt
     2161 atctgacgct gaagtcgctc cagcgtcagt aattgcaatg ttatttgcta ctgtccgggc
     2221 cgcgttcacc ggttgctacc ggattttttg gatcgctgct ccattcgtac cagccgccgt
     2281 catacacgga aacattcttc caacccatgg cgcgtgcgta cataaaggtt tcggacgcgc
     2341 gccagccggt gccgcagtag aatgaaactt gctgctctgg tttgatattc cacgctttcc
     2401 acatagcggt aatatcatcg gcgctgcgca tggtgccatc cgggttatga aaatcttcca
     2461 tatgcgtcga gtcgctacca gcgtgtcccc aacgtgctcc ggctatttca ccttttggtt
     2521 taatatagct gtaaccgctg gtcgtaccga taaattctgg ccacgaacga atgctcacca
     2581 gcgatgcatc ctggcgatgc agcagtccac gggcctgttc catatcgagc atcaactgcg
     2641 gttgtgccgg gatcttcacg ccgaaatccg gttccgcttt cactttcggt ggcgttcctc
     2701 gctcaacagg cagtcccgcg tcggaccagg tttgccagcc gccatccagc agacgcacat
     2761 ctttcacgcc tgcgtagagc ataatttgcg ccacacgcgc tccagcgtat acgtcacgcc
     2821 cgtacagaat gaccgtggtg tcatggcgaa tgccgtgttt tgccagcatc gcttttagtt
     2881 gttcatcaga aactttgttc cacagcggtt cgctttccac ttcgttggta tcgatgtagt
     2941 cagcaccggg aatatggctg ataaggtaaa gcttaggagc gccccaggcc gcttcaatga
     3001 ctttccagtc accggcaggt ttcgccgtaa cctctttacc ttgttgcagg tcgtgcagcc
     3061 attgcggata aaccagctgc tcaaagtgcg gcagtttttg tagacgggaa ggttcgctta
     3121 gcgcgtcact cagaatggag atatgcgtaa aacctgcttt ttgcagtcgc gttttgacgg
     3181 cgtcgacatc tttgtcatta ccgtacagcg ccaccggggc atcggctttc aggttatgtt
     3241 gcttgatcca cgcgttgagc tgttcggtgc tcattttgtc gagccagctg gcagagaggt
     3301 ttaaggcggc aggttcatga ccagaagggc catttaaggt ttgtggccag ccgttataaa
     3361 acgcgctggg gcgagtatct atcgctttgc cattttgttg ttgaagctgg tcaagtgtaa
     3421 gaggcttcgc cagttcagcg gcccacgaag aagcgcaagc cagccctaaa gccattgcca
     3481 gcgcggtcat ttgagaaaca cgtttcatcg aaaatcccga cattgtagtt ttgagtgcgc
     3541 cattctgaac tgacgcattg agaaaaactt tgcgttatcg cagtttgttg taattttctg
     3601 accactgcgc catatccaga acagaactat cagcaggaac atcctggaga tcgtgcgtta
     3661 cctgaacgac ggggatcgcc agttcgcgaa cttcgctgaa cacccactgg cgaaaattat
     3721 cgcgcagagc cacatcaaga cggctgaatg gctcatccag gagtaacgct tttggctgag
     3781 cgagaagggc gcgcagcagg gccacccgcg ctcgctgacc gcctgataat gtcgcagggt
     3841 cctggtgata agtttcagcc agtcccgctc gatcaagcgc gtctttcacg gcattgcgcc
     3901 tggcagtccc cttgagcgtc gacggtagcg ccagcagtaa attttgcccg acgctgaact
     3961 ggtcaaataa cagtgcatcc tggaaaagaa tgccaatctg acgctgtgcg gtgggtagca
     4021 tgtcaatccg ttgctcattg agccatagct cacctgtaca agaaaactgt ccagccagtg
     4081 caccaatcat ccatgaaaac agagtggatt ttccacagcc agacggcccc attaacgtga
     4141 cgatgtcacc tttatcaacc gtaaagttaa cgtttgtcag caagcggctt tctggtaaac
     4201 gtagcgaaac atttttcacg cagagcatta gcggagtcct tgtctgacat aacctaccca
     4261 ttttgcggct aacgcggtca gggcaaaaat aataagcggt aacaacagtt gccataaagc
     4321 ctgggcggcg agaataccgt tgctgccgcc gctgcttaat gccaccgcct cagtggtgag
     4381 cgtcggaaaa cgccccgcgc ccagccacag cgtcggcata tactgcgcaa tactgactga
     4441 aaatcccacc gcgaaggcaa tcagcacagg gcgcaacatg agtgtgcatt tcacgtaaaa
     4501 gaagattttg gcccgcgacc agcccagtgt ttgcgcaatc aaaattaacc gtgaatcaat
     4561 gcgctgccag gcgggttgca ggataaacag catccacggc attacccaca gcagatgccc
     4621 ccagaccacc gccgtccagc ttccatccag gttcagccat agcgccagcg tgtactggcc
     4681 cgccaccagt ggcagagcgg gcaatagaat gggcaaccat agccacaact ggcgacgctg
     4741 tggcccccat tccagccaca gcagtaacag gagcaaagcg atgaatgttg ctaccagccc
     4801 cattgtcaga ctgttgatga gtgcttcact attgatcgtc gactgatccg cgaggatcgc
     4861 cagcagaacc acacacagca caccggttaa gggtaaaaaa ctcgccagcg tagtgcctgg
     4921 taataaaggt gtggcaggct tgcgaatgcc atctacgcgg ggaatagtgc gccgccagct
     4981 gcgccatagc agatagctca gcaaaacgta ggcggcgagt aacagcatca gcagcagact
     5041 ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga gttaaccact gccagctaat
     5101 tactgccagc gtcggtggat tacctggccc aagaataatc gccacatcca cgaccgaaag
     5161 cgaccaggca acaatcgcca gcatcgccat tgccagcgaa ggcgcgacgg agggcaacag
     5221 cagccagttc aggcattgcc agcggctgta gcccagcgaa tccagcacaa tgacctgctg
     5281 caacagccgt ttttcgctca atactgccgc taagatccac agcagaaatg cgctttcttt
     5341 cactgcaagg gtgaggccca gaccgatgcc aaatcggtcc attggcggag tgaaatacgg
     5401 gaaatagtca tatagcagcc ctccgtcagc aaagagcagc agggcgctgg tggcaaaagc
     5461 cacatgggga atggcgagca gccacggcag acgggcgcac atacgctgcc atttcggccc
     5521 aggccatagc gccacaatca ccagcagggc aatcaacaat gccccgaccg ccgcgatggt
     5581 tgtcgacacc agcgttgcca gtaatgcatg cggtaactgt ggatcggcaa ataacgcctg
     5641 ccagtgtgtc aacgacaacg caggcgatat cagcgtgaga gctgccggga tcagcggtgc
     5701 ataaatcacc gccaccatcg cccacagcaa aaagattaat gcgtaccgta acggcgtaac
     5761 cattcttgtt ccagtgcatt cacccatcct gcgtgcggtt cagccagtac cggcggcaga
     5821 tcctgcggca ttcttgattg caatatttcg cgctgcccgt caggcagttt ttgcggatcg
     5881 agaacagaag gatcgtccca gacaacggga tctgctttac gcagttgcgc atcgggtgag
     5941 agcagaaaat tggcaactac cttcgccgca gcactggcat tcgcgttggc tggaatggtg
     6001 acgaaatgca cgttgccaat catcccctcg cgaaagccaa aactgtaact gcttgccggc
     6061 aaatcgccgc tggcgatttt ttgctgcgca tgcgcggggt taaaggtcag cgacaggcgc
     6121 aatgtgccgg ctttcagcag agcatccatc cgcgcgggcg aaggcgggaa atcctttcct
     6181 tcgcgccaca aatacggatg cagtgcatca agatattgcc acaagggcgc agtgacacgg
     6241 gcgaaagtcg catcgtccgg cgcttctttt aatgcggcgg gatctggcgt cagcataatc
     6301 agcaactgtt caagaaacgc cgtgccggta aagtccggtg ggcgcggata agtaaccgtg
     6361 ccgggattgg ctttagcaaa ctccagtaag gcttgcggcg tttgtggtgg ctgtggcgta
     6421 acatcgcggc gggcgataaa cgtcagttgt gcgccgcccc agggcgattc cgcaccttgt
     6481 gtcggcactg aaaaatcttc ccgcaccggc agctgtgtgt cgacatagcg ccagttgggc
     6541 agagtctccg cccagcccgt ttgtaataaa ttggcctctt ttaaggtgcg gaagttttcg
     6601 ccgttcaccc agagcagatc caccgagccg cccgttttac gaccggcagc agcttcggtc
     6661 tgaatgcgct tcaccgcgtc tgcggcatcc gcgagacgaa caatcttcag gtttatagcg
     6721 taatgggttt tcatctcgcc gctaacccag tcgagatagc ggttaattgc ggtatcgccg
     6781 ccccaggcgt taaaccagac ggtttgccct ttggcctcat ttttaatttc ctgccagtca
     6841 ctggcatgtg ttgccaaaga aaacagcgat aacaatccca gcaaccaccc acaatggcgc
     6901 atcttctctc cttaagaacg tttttccgga atacggcaca gcccctgagc gggaatgtcc
     6961 atggcagaat taaaacgagc cgacaggttt tgcaggccga tcaatgccgt cagttcggtg
     7021 agcgcctgag cgtcaaaatg cgcagccagt cgggtacgca gggcatcatc gactgttggc
     7081 ggtgtgacgc ttgcggcttc ggcgtactcc agcgccagcc gttcttcatc gctaaagagc
     7141 gggctttgcc gccagtcagc cactgccagc agtttatcgg tgctgccggt acgctcggcg
     7201 actttcatac tggtgatatc cacacaaaac tcacacaggc acatttgcgc aatgcgcgcg
     7261 ctgacaagcg atcgcactac cggatcgagc ggtgagcgtt tgcgctccag ccacccaaca
     7321 aacattgaca ccaaataaaa gataaacggg atccgccccc accagcgaat cgggcttagc
     7381 acctcgccgt aatggcgacg atgcagccac gcctgcggac gtaaaatgaa aggaattttg
     7441 ctaagcggcg gtaaacccat cagtcattcc tcttctggcg ctcaagccat attttcttcg
     7501 ccataaaaat caccacggtc agggcaaaca gaataaacag tccggtgaca aaccagaaag
     7561 taccgcctgt taacatgctg cccgcccagg aataaacaat agtcgccggt aattgaccaa
     7621 gcccggtggc gataaaaaac gagcgaaaac gtattgaagt cagaccggca gcatagctga
     7681 ttggatcgaa agggacaaaa ggcaataacc gacagaccag gatggtgtgt ttgccgtagc
     7741 gagtgaaaaa gccgtccata ctgtcaagca cggttttgcc ggttaatttt tccaccactt
     7801 cgcggcccat cactctggcg ataaaaaagc acagcgccgc gccggccatc gaactggtcc
     7861 acgacagcaa gccgccccag aacgcgccaa acagcgacgc attggcaaag gtgatcaaaa
     7921 acgcaggtag cggtgcagca atggcctgta aaatcatcaa caagaacgag acaactgccg
     7981 ccagtgcgcc gtaagactga ataaagcgtt ctataccctg ttggtccacg gcggcaaacg
     8041 ccgcaacgct gcgattgata aactcatgga cgccgggaag cagcgcccat gcgagcagca
     8101 tagcgaacaa caggatgatg aggg
//
LOCUS       DAJTYD010000169         8095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTYD010000169 DAJTYD010000000
VERSION     DAJTYD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.169"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..190
                     /locus_tag="OO585_003361"
     CDS             <1..190
                     /locus_tag="OO585_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630840.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OO585_003362"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OO585_003362"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6630841.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OO585_003363"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OO585_003363"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6630842.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO585_003364"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO585_003364"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6630843.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OO585_003365"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OO585_003365"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6630844.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OO585_003366"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OO585_003366"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6630845.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OO585_003367"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OO585_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6630846.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OO585_003368"
     CDS             7144..7353
                     /locus_tag="OO585_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6630847.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO585_003369"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO585_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6630848.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1839 a         1978 c         2211 g         2067 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaag
//
LOCUS       DAJTYD010000170         8039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTYD010000170 DAJTYD010000000
VERSION     DAJTYD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.170"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..825)
                     /locus_tag="OO585_003370"
     CDS             complement(<1..825)
                     /locus_tag="OO585_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6630849.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDY"
     gene            917..1003
                     /locus_tag="OO585_003371"
                     /pseudo
     CDS             917..1003
                     /locus_tag="OO585_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1304..1393
                     /locus_tag="OO585_003372"
                     /pseudo
     CDS             1304..1393
                     /locus_tag="OO585_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1417..5931
                     /locus_tag="OO585_003373"
                     /pseudo
     CDS             1417..5931
                     /locus_tag="OO585_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5932..7581
                     /locus_tag="OO585_003374"
     CDS             5932..7581
                     /locus_tag="OO585_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6630850.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7586..>8039
                     /locus_tag="OO585_003375"
     CDS             7586..>8039
                     /locus_tag="OO585_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630851.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDT"
BASE COUNT         1963 a         1925 c         2259 g         1892 t
ORIGIN      
        1 gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt
       61 gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt
      121 cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg
      181 tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg
      241 taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt
      301 aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc
      361 caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag gtaaacatgt
      421 taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct
      481 gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga
      541 gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga
      601 gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac atattttcag
      661 taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca
      721 gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa
      781 accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc
      841 attgtttata tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgctcc
      901 tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt
      961 gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt
     1021 catcaagcca gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag
     1081 catggttgta aattcaacta acccagtgcg ttgacaaaaa attagcgcaa gaagacaaaa
     1141 aatcaccttg cgctaatgct ctgtatcagg tcactaatac cagctaagta gttgattcat
     1201 agtgactgca tatgttgtgc tttgtcgcat tttgtagtct attatttaat ctgtaacaac
     1261 gataatacaa tgaattttaa ttggtttttg tgattttgtc cttcttctga catggaagga
     1321 cacccgatgg atacccaacg attccaatcc caatttcatt ggcatttatc gtttaaattt
     1381 tctggcgcga tagcagcgtg cttgtctctg tctcttgtgg gaacagggct tgctaatgct
     1441 gatgattcgc ttccttccag taactatgcg ccgcccgccg ggggaacatt ctttttgctt
     1501 gctgacagca gttttggcag cagtgaagag gcgaaagtgc gactggaagc gccggggcgt
     1561 gattatcggc gctatcagat ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt
     1621 cctgacccga tggcattttt gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg
     1681 caatatctgg gcgacgggct gaacaatacg ctgacctggc tgtgggataa ctggtacggc
     1741 aaatctcgcc gcgtaatgca gcgtactttc tcttctcagt cacggcagaa tgtgactcag
     1801 gcattacccg aattacatct cggcaatgcc attattaaac cttcccgtta tgtacagaac
     1861 aaccagtttt ccccgctgaa aaaatatccc ttggtggaac agttccgtta tccactatgg
     1921 caggctaaac cggtcgagcc gcagcaaggg gtaaaactgg aaggcgcatc cagcaatttc
     1981 atctcgccgc agccgggtaa catttatatt cctctcggcc aacaagagcc gggactgtac
     2041 ctcgtcgagg cgatggttgg tgggtatcgg gcgacgacgg tggtgtttgt ttccgatacc
     2101 gtggctctta gcaaagtgtc aggcaacgag cttctggtgt ggaccgcggg taaaaaacag
     2161 ggtgaagcga agcccggctc agagatcttg tggactgacg gtcttggcgt gatgacccgc
     2221 ggtgtgaccg atgacagcgg taccttgcag ttacaacata tatcgccaga acgttcatac
     2281 attctgggta aggatgctga aggcggcgtt tttgtctccg agaacttctt ctacgaaagc
     2341 gaaatctaca acacccgctt gtatattttt accgatcgcc cgctatatcg cgcaggcgat
     2401 cgtgtcgatg ttaaagtgat gggccgcgag ttccacgatc cgttgcattc atcccccatc
     2461 gtcagcgccc cggcgaagct ttcggtgctg gacgctaacg gcagtctgtt gcaaaccgtc
     2521 gatgtcacgc tggatgcgcg caatggcggg cagggaagtt tccgcctgcc agaaaatgcc
     2581 gttgccggag gttatgagtt acgtcttgct taccgcaatc aggtctatag cagcagtttt
     2641 cgcgtggcaa actacatcaa gccacatttc gagattggtt tagctctcga caaaaaagag
     2701 ttcaaaactg gcgaagcggt cagcggcaaa ctgcaactgc tctacccgga tggcgagccg
     2761 gtaaaaaatg cccgcgtgca gttaagtttg cgcgctcagc aattatcaat ggtcggtaac
     2821 gatttgcgtt atgccggacg tttccccgtg tcgctggaag gcagcgaaac ggtgtccgac
     2881 gccagcggtc atgtggcgtt aaatctcccc gccgccgata aaccgagccg ttatttgtta
     2941 accgtctccg ccagtgacgg cgcggcgtat cgtgtcacca ccaccaaaga gatcctcatt
     3001 gaacgcggtc tggcgcatta ctcattaagt actgccgcac aatacagtaa tagcggcgag
     3061 tcggttgtgt tccgttatgc cgcgctggaa tcttcaaaac aggttcctgt tacgtatgaa
     3121 tggttgcgtc tcgaagaccg cacgagccat agcggagagc taccgtcagg cggcaaatcc
     3181 tttaccgtca atttcgctaa acctggcaac tacaatctga cattacgcga taaagacggc
     3241 ttaattctcg ctgggttaag ttatgccgtc agcggtaagg gcagcacggc gcatacgggt
     3301 acggtagata tcgtggcgga taaaacgctg taccagccag gcgaaaccgc gaagatgctg
     3361 attacctttc cggagccaat tgatgaagca ttattgacgc tggaacgcga tcgcgtggaa
     3421 cagcagtcgc tgctttcgca tccggcaaac tggctaacgc tacaacgttt aaacgatacc
     3481 cagtatgaag cccgggttcc agtgagcaat tcctttgcgc ctaacatcac tttttcggtg
     3541 ctgtataccc gtaatggtca gtacagtttt cagaacgccg ggatcaaagt tgccgttcct
     3601 cagcttgata tccgggtgaa aacggacaaa acccattacc agccaggtga actggtcaat
     3661 gtcgaattaa cctcgtcgct gaaaggtaaa cctgtttctg cgcagctaac ggtaggcgtg
     3721 gtcgatgaaa tgatctacgc gctgcaacca gaaatcgcgc cgaatatcgg caaatttttc
     3781 tatccgctgg ggcgtaacaa tgtgcgtacc agctccagtc tgtcgtttat cagctacgac
     3841 caggcgctct ccagcgagcc ggttgcgcct ggcgcaacta accgcagcga gcggcgagta
     3901 aaaatgcttg aacgtccacg gcgtgaagag gtggataccg cggcatggat gccgtcactc
     3961 acaaccgata aacaaggcaa agcgtatttc acgttcctga tgcctgattc gttaacccgc
     4021 tggcgtatca ccgcgcgtgg gatgaacggc gacgggctgg tcgggcaggg gcgtgcttat
     4081 ctgcgttcgg aaaaaaatct ctacatgaag tggagtatgc caacggtgta tcgcgtgggc
     4141 gacaaaccgg cggcaggact gtttatcttc agtcagcagg ataacgaacc ggtggcgctg
     4201 gtgactaaat ttgcaggcgc tgagatgcgc cagacgctga cgctgcacaa aggggcgaat
     4261 tatatttcgc tggcgcagaa tattcagcaa tctggcttgt taagtgcaga actgcaacaa
     4321 aatgggcaag tgcaggacag cattagcaca aaactgtctt ttgtggacaa cagcaggccc
     4381 gttgaacagc agaaaaatgt catgctcggc ggtggcgaga acgcgctgat gttgcccgag
     4441 caggcgagta atatccggct acaaagtagt gaaacgccgc aggagatttt ccgcaacaat
     4501 cttgatgcgt tagtcgatga accgtggggt ggggtgatca acaccggtag ccgtctgatc
     4561 ccgctcagtc tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt
     4621 cgtcagatga ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc
     4681 tttacctggt ggggtgaaga tggcaatggt gacgccttcc ttacggcatg ggcatggtac
     4741 gccgactggc aggccagcta ggcgctcggc gtaacgcaac aaccggaata ctggcagcat
     4801 atgctcgaca gttatgccga gcaggcggat aacatgccgt tattgcatcg ggcgctggtg
     4861 ctggcgtggg cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa
     4921 gctatcgccc ggcgcggaac taaagatgaa gatttctctg aggaagacat ccgcgatatc
     4981 aatgacagcc tgatcctcga tacaccggaa tctccactgg cagatgcggt ggcaaacgtc
     5041 ttaaccatga cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag
     5101 caatatgcgt gggataaagc ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg
     5161 ctcaatagcg gtggtgacgc tacccagacg gccgctattt taagtggttt gaccgctgag
     5221 caatccacta ttgagcgcgc gctggccatg aactggctgg cgaaatatat ggcgacaatg
     5281 cctccagttg ttttgcctgc gcctgcgggc gcatgggcta aacataagtt aactggaggg
     5341 ggcgaatact ggcgttgggt tggtcagggc gtgccggaca ttctctcttt tggtgacgaa
     5401 ttatcgccgc aaaatgtgca ggtccgctgg cgtgagccgg caaaaatggc tcaacaaagt
     5461 aacattccgg tgaccgttga acgccagttg tatcggctta tccctggtga agaagagatg
     5521 agctttattc tgcaaccggt gaccagcaat gagattgaca gcgatgcgct gtatctcgat
     5581 gacatcacgc ttaccagcga gcaggatgca gttctgcgct acggtcaggt ggaagtaccg
     5641 ctgccaccgg gagccgacgt tgagcgcaca acatggggca tttcggtcaa taaacccaac
     5701 gtcgcgaaac agcaggggca attgctggaa aaagcgcgaa atgaaatggg cgaactggcc
     5761 tatatggtgc cggtgaaaga actgacggga acggtcactt tccgccattt gctgcgcttc
     5821 tcgcaaaaag ggcaattcgt tctgcctcct gctcgttatg tgcgttccta tgcacctgca
     5881 cagcaaagtg ttgcggcagg gagtgaatgg accgggatgc aggtgaaata agtgaactgg
     5941 cgcagaatcg tctggctatt ggcgttggtt actcagccaa cgcttgcgga ggagacgccg
     6001 ttacaactgg cactgcgtgg tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc
     6061 gtgaccacag tctcagcatt acctgactcg ctcaccacgc cgttagggag tttgtggaaa
     6121 ctgtatgttt acgcctggct ggaggatacc catcaaccgg agcagcccta ccagtgtcgc
     6181 ggaaactccc cggaagaggt ttattgttgt caggctgggg agagcataac gcgcgatacc
     6241 gcgctggtac gttcctgtgg gctctatttt gcgccgcagc ggttgcatat tggcgcagat
     6301 gtgtgggggc aatactggca acagcgtcag gcgcctgcgt ggttagcgtc tcttactacg
     6361 ctgaagccag aaacctcggt aacggttaaa tcgctgcttg attcattagc gacgttacct
     6421 gcgcaaaaca aagctcagga agtcttactg gatgtggtgc tggacgaggc gaaaatcggt
     6481 gtcgcatcca tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat
     6541 aaacaggaaa tccgtcaggg tggttttgcc ggctggttaa ccgatggtac accgctgtgg
     6601 gttactggca gtggcaccag caaaacggta ctaacccgtt atgcgacagt gcttaatcgg
     6661 gttttgccag tgccgacgca ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc
     6721 cgctatccgc tgaagaaaat cacagcggaa aagagtacga cggcagtaaa acccggcgta
     6781 cttaatgggc gctatcgtgt gacgtttact aatggtaacc acattacgtt tgtcagccac
     6841 ggtgagacga ccttactgag cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt
     6901 gaagagtacg ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc tgaggcagcg
     6961 aaagccatga ccgtggcgat tcacactttt ttacagcaaa acgccaatcg ggaaggcgat
     7021 tgcctgacca tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg
     7081 ggggcgcgaa cgatgaccgc ctggacgcag gatctcatct atgcaggcga tccggttcat
     7141 tatcatggca gtcgagctac cgaagggaca ctttcctggc ggcaagccac ggcccaggcg
     7201 gggcagggag agcgttacga ccagatactc gcgtttgctt atcccgacaa cagccttagc
     7261 cgctggggag cgccacgctc aacctgccag ttattaccca aagcgaaagc ctggctggcg
     7321 aaaaaaatgc cgcagtggcg gcgtatatta caaggcgaga cggggtacaa cgaaccagac
     7381 gtgtttgcgg tctgtcgttt agtctcaggt ttcccctata ccgatcggca gcagaaacgg
     7441 ctgtttattc gtaacttctt tacgcttcag gatcggctgg atttaaccca tgagtatctg
     7501 cacctggcct ttgatggtta tcccaccggg cttgatgaga actatatcga aacgctgacc
     7561 cgacaattat taatggacta atgctatgcg aaagattttt cttccgttgt tactggtggc
     7621 actttcgcct gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg caccgctctc
     7681 tggctggcat ccggcagagg gcgaagatgc cagtttttca caatcgatca attacccggc
     7741 gtcgtccgtc aacatggcag atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa
     7801 gaattatgct gcggcgggta aaattcagca gggccggctg gtggtcaacg gtgccagtat
     7861 gccgcagaga gttgaatccg atggttcatt tgcacgccct tatattttca ctgaaggcag
     7921 caacagcgtg caggtcatca gcccggatgg gcaaagccga caaaaaatgc agttttactc
     7981 aacgccgggt acaggaacga ttcgtgcacg tttacggctg gttctctcgt gggatacgg
//
LOCUS       DAJTYD010000171         7903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTYD010000171 DAJTYD010000000
VERSION     DAJTYD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.171"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(122..853)
                     /gene="dnaQ"
                     /locus_tag="OO585_003376"
     CDS             complement(122..853)
                     /gene="dnaQ"
                     /locus_tag="OO585_003376"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6630852.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            918..1385
                     /gene="rnhA"
                     /locus_tag="OO585_003377"
     CDS             918..1385
                     /gene="rnhA"
                     /locus_tag="OO585_003377"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6630853.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1382..2104)
                     /locus_tag="OO585_003378"
     CDS             complement(1382..2104)
                     /locus_tag="OO585_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6630854.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2138..2893
                     /gene="gloB"
                     /locus_tag="OO585_003379"
     CDS             2138..2893
                     /gene="gloB"
                     /locus_tag="OO585_003379"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6630855.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2965..4323
                     /gene="mltD"
                     /locus_tag="OO585_003380"
     CDS             2965..4323
                     /gene="mltD"
                     /locus_tag="OO585_003380"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6630856.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4372..4968)
                     /locus_tag="OO585_003381"
                     /pseudo
     CDS             complement(4372..4968)
                     /locus_tag="OO585_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(4999..5799)
                     /locus_tag="OO585_003382"
     CDS             complement(4999..5799)
                     /locus_tag="OO585_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6630857.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6040..6954
                     /locus_tag="OO585_003383"
     CDS             6040..6954
                     /locus_tag="OO585_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6630858.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(6951..7744)
                     /gene="dkgB"
                     /locus_tag="OO585_003384"
                     /pseudo
     CDS             complement(6951..7744)
                     /gene="dkgB"
                     /locus_tag="OO585_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2106 a         1898 c         1919 g         1980 t
ORIGIN      
        1 cgttgggaac gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc
       61 ttttgaatcg tttgctgcaa aaatcgccca agccgctatt tttagcgcct tttacaggta
      121 tttatgcccg ccagaggcag cttccgccct tcttctccac cagatcaaga cgggcttcat
      181 gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa
      241 tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc tccttccatc gcaaaagcca
      301 tcgacgtttg accaccggtc atcgccagat aaacttccgc aaggatctgt gcatcgagta
      361 atgccccgtg tagcgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga
      421 ggctgttgcg cttaccagga aacattttcc tcgccaccgc aaggctatcg gtgaccttac
      481 agaaggtatt ggtcttcgga atatcgcgct taagcaacga aaactcgtag tccataaagc
      541 cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcggatatag tccatgaact
      601 catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta
      661 cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc
      721 ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat
      781 agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa
      841 ttgcagtgct catagcggtc atttatgtca gacttatcgt tttacagttc gattcaatta
      901 caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg
      961 gcaatccagg acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct
     1021 ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg
     1081 cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc
     1141 gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa accgcagaca
     1201 aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcccta gggcaacatc
     1261 aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg
     1321 aactggctcg tgctgcggca atgaatccca cactggaaga tacgggctac caagtcgaag
     1381 tttaagcttg tggtttacga cattgccggg tggctccaac cgcttggcga attcgtggct
     1441 tgtttttact ctgtttcatc gggtttagcg ttaaaggaat agtccgtttc cgggcaacaa
     1501 taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa tagttttcct ccgtgtttgt
     1561 tccacgggag aacgtggaaa cggctggcgt gcagcacttc aaaattcaac aaagagagcc
     1621 agtccagttg ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt tttcgcaata
     1681 ccggcacaag tttgcgtaat cccattaaac taatgggatt gaagccacta atcaccagcc
     1741 agccatcatc aatcaatacc cgatcggttt cacgcaataa acgatgcgga tcggtgcacc
     1801 acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa
     1861 gtggatccgc ctgcacctgg acgggcattc cttgcgcaga aacattcact tgatgagaaa
     1921 ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agatgaaaac
     1981 catacatttt agtgaaccat gggttgagct ggcgctccag cgccttgcga taaagctctc
     2041 cccagggcaa atcgccccag cgatcaggag ccacgacagt ttgagggact cttgccggtt
     2101 tcatcacaac cttccgtttc acactaagag gtaatctatg aatcttaaca gtattcccgc
     2161 ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga
     2221 tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc aaccggaggc
     2281 catatttctc acccaccatc atcacgatca cgttggcggc gtaaaagaac tggtggaaaa
     2341 gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa caacacaggt
     2401 agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc
     2461 gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac
     2521 gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca gcatcacaaa tgtatcaatc
     2581 acttaataag ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac
     2641 gttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca taaatgatta
     2701 ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata acactacccg taattctgaa
     2761 aaatgagcgg caaattaatg tttttttaag aacggaagat attgatttaa ttaatgtaat
     2821 taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa ggtcaaagaa
     2881 agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc
     2941 ttttaagcaa ctattgacac acacatgaag gcaaaagcga tattactcgc ctctgtcctg
     3001 ctcgtgggtt gccagagtac cggcaacgtt caacagcacg cacagagcct ttctgcagct
     3061 ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga tgggacgtct
     3121 atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat gggaattccg
     3181 gaaaatgacc ggattcgcga acagaaacag aaatatttac gcaataagag ctatctccac
     3241 gatgtaactt tacgggcaga gccgtatatg tactggatag cagggcaagt taaaaaacgt
     3301 aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga tcctcacgca
     3361 acgtctggcg ccaatgccgc aggcatctgg cagatcattc cgagcacggg gcgcaattat
     3421 ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc
     3481 gcgctgaaca tgatgcagcg tctgaacaag atgtttgacg gcgactggct tctgaccgta
     3541 gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt
     3601 gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct
     3661 aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt acgtctgcca
     3721 acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga gcagcccggt tgaaatggcg
     3781 aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa cgctggcgta
     3841 aaaggctcca cgctgggcgc aagtggcccg cagtacgtga tggtgccaaa gaagcatgca
     3901 gatcaactgc gtgattctct cgcttcaggc gaaattgctg ctgtacagtc gacgctggtt
     3961 gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga cacgctttca
     4021 agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa caaactgcgc
     4081 ggatctaagc tgaagccagg ccaaagtttg acgattggtg caggcagtag cgcacagcgg
     4141 ttggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc gctttcaagc
     4201 attgctaaac gccacggcgt gaacatcaaa gatgtgatgc gctggaacag tgacactgcg
     4261 aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacaacat gccagattcc
     4321 tgacaaacca gataataaaa aaggcaccga ttcccccggt gcctttttta tttatgccgc
     4381 tttatgcgct tctaccatga tgatatcact ggtgaaagag ccatcagtct gcaaggaaaa
     4441 atacgttctc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc
     4501 ttctggcgta cgcattctca cgacccatga agaaaattcc agcggtaact tatctgtaat
     4561 taaattatca actatcagat tggcttcatt gattaacgtc aaccacgcac cgctggcgta
     4621 gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc tgtaaccaga tgtcgcgcac
     4681 tgggtgaccc ggagacatta catccatcac aatcagccta ccgccaggtt tcaatatcct
     4741 attcacttct cgcagtgctg caccaacatc atgccagtga tgggcagaat aacggctgat
     4801 aacaatatca aatgcgttat cggcaaatgg cagacttgcg gcatatccct ggcgggtggt
     4861 gatatttttc agttgccggg cttcggcagc ttgtgccaca acatccagca tgtgggccga
     4921 taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg ccaaacgttg gtctgccctg
     4981 tggcagacct gacatacctt atttatcagg cttgccggga ctgaattcaa cgagtagcgg
     5041 attgtgatcg gaagcgcgcg taaccagtac agaagcttca ctgacgttca gaccacggta
     5101 gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac
     5161 ctggcgcagc gacatttccc gcgcaaagcg atataacgcg ttcatccttc tacggctcca
     5221 ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg tgagctatct gatcaccaat
     5281 aggaagtaac tgcttactat agacatccac gcccagactg aagttgacgg cgtgtatatt
     5341 aaccaccatc aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg ccgacttcgc
     5401 cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa tacactggat gtgccgccga
     5461 aagggtcatt acgccagaag gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc
     5521 aagatagtta gcggtcgcaa actgtactaa ctctggcgtt gtctgcgctt cctgcaataa
     5581 caccaggtgt gcatctttgc cgtagttctt taataccgac aaccattcag cgcgttgctg
     5641 tttgtatatg ttccacacca ggatccgaat acgctcttcg gtacttaacg gttccccagg
     5701 tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt aagatccttt ccgcaggttg
     5761 tccggcaaca tagcgcatgg cataggtgtt ttttcgcact tttgactttg aaacctctat
     5821 tacgtcaacc cttccgcact cgggagggtt ctactattat agggatttta gctcactctt
     5881 tcaacgagca attgtatatt gttatgtaag caagtgctta caccggtcag aattttgctc
     5941 attatggaga acattaaatc gcatgacata tcacgcatcc tgatggttta atgacaaata
     6001 aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa
     6061 ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta
     6121 gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc tggagatgaa acttggcgtt
     6181 agcctgctta atcggaccac gcgacaactt agcctgacgg aagaaggcga gcgttatttt
     6241 cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag aatcagaaat tatggagacg
     6301 cgtaatacac cgcgtggact gttacggatc gatgccgcaa ctccagtggt gctgcacttt
     6361 ctgatgccgt taattaagcc tttccgtgaa cgctatccgg aagtcacttt gtcactagtc
     6421 tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat acgcgctggt
     6481 acgttaacgg attccagctt acgtgccagg ccgttattta acagttatcg aaaaattatc
     6541 gcctcccccg attatatttc ccgctacggg aagccagaaa cgatcgacga tttaaagcaa
     6601 catgtttgcc tgggattcac tgaacccgct tccctcaata cctggccgat agcctgtagt
     6661 gatggacaat tacatgaggt gaaatacggt ttgtcatcca atagtgggga aacactgaaa
     6721 cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg actacatgat cgacagagaa
     6781 atcgctcgcg gagaattggt ggagttaatg gcagataaag tgttgccagt ggaaatgcca
     6841 ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc gcatccgggc ttttatcgat
     6901 ttccttagcg agcatgtaaa aacagctccc ggaggagctg tcagagaggc ttaatcccat
     6961 tcaggagcca gaccttccgg gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct
     7021 tttttatctt cggcatcaag ctgtaaattt tgtgccttaa gattactttc caggttttta
     7081 cgtttagtag aagaaggaat tactgagtaa ccttccccca tagcccacgc cagaatcact
     7141 tgtgccggag tcgcattgtg tttagctgcg atacgagcaa taacctcatc tttcagggcc
     7201 ttaccatacg ccagcgtcat ataggaagta atatgtatgc cgtgctgttt agcccaggca
     7261 accactttac ggttttgcag ataaggagag agttcaatct ggttagtagc gatgttttca
     7321 gcgccaacag cagcaatcgc cttttccatc aatgggatcg tgaagttgga aataccgatc
     7381 tcacgcgtca gcccttgttt tttggcttcc agcagcgcct gcataacccc ttcaacagag
     7441 acttcatcgt ttggtgacgg ccagtggatt agcgtcagat caacataatc ggtacgcaat
     7501 ttttgcaggc tctctttcag acttgggatc aatttgtctt tgctgagatt ttcaatccag
     7561 attttagtgg tgatgtagag ttcatgacgt ggcacgccac tttctgcaat cgcctgacct
     7621 actgcggctt cgttatcata gatttgtgcg gtatcaattg cgcgataacc aagttcaagc
     7681 gccgttttca cagatgaaat aacaacgtag tctttcaacc taaaccaaat gcagggatag
     7741 ccataatatg cctcttttaa tgctattttc gttaactgag gtgattatgg gcttcagact
     7801 gttgaggaaa aaagcgtaaa atgcagagga tttttgcgat tctggcaata atagatatac
     7861 agacgaaaaa aaacctcgag cgttaactcg aggcttctta ata
//
LOCUS       DAJTYD010000172         7850 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTYD010000172 DAJTYD010000000
VERSION     DAJTYD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.172"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(95..1765)
                     /gene="hscC"
                     /locus_tag="OO585_003385"
     CDS             complement(95..1765)
                     /gene="hscC"
                     /locus_tag="OO585_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6630859.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKQEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="OO585_003386"
     CDS             complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="OO585_003386"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6630860.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="OO585_003387"
     CDS             complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="OO585_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6630861.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="OO585_003388"
     CDS             complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="OO585_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6630862.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="OO585_003389"
     CDS             complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="OO585_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6630863.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="OO585_003390"
     CDS             complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="OO585_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6630864.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6574..>7850
                     /locus_tag="OO585_003391"
     CDS             6574..>7850
                     /locus_tag="OO585_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6630865.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVT"
BASE COUNT         1959 a         1954 c         1941 g         1996 t
ORIGIN      
        1 atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg
       61 gatgcagcgt ttacgccgca tccggcagcc gtgtttatgg gatctcaatg gctaaataat
      121 cgctcgcccg ccgccgaact tcatccacgc gggccatgtc gtttgactgt agcgccgcat
      181 caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgct cgcgcccaca
      241 actcttcctg tttggcttta aaggtgcgat tgatgagcat atcgcgcgga taaattttca
      301 atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc gctcaatgtt accgggctgt
      361 ggttaatcac tctggactta acgctgccgt cttccagaag cacgtcaact tcaagcaacc
      421 cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg
      481 gcacatcgaa ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga
      541 cgttaaccgt aattgaatcc tgctccgggt gcatggtcga gtaagtttct acacgcgaca
      601 ccggtactgt ggtgtttcgt tcaataatcg gcgagaaaat gccggaaacg ccctggcggt
      661 taacttcaac gcccaacgag taagggcaaa tatcagtgag gattacctct tcaatatctt
      721 cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcggat
      781 cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca
      841 tttgtgacgc gccgccaacc agcaccagac tgtcgatttg actcggcttc agacgcgcat
      901 cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt
      961 ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcttc ctgatactgc cagcgaatgt
     1021 gcaatggcga ttgattgcta cattttgccg cttccacaca ggcgtacaag gctgccagtt
     1081 cactctcgtt aagcgtggtc ctggcaactt ccgcgcgttt taaaacctca tcgaccagca
     1141 tatgggtaaa atcttcgcca ccgaggaagc tatcgccagc ggaggcgtga atttcaatca
     1201 ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaaaacca
     1261 gcgagcgggt attttgttgc gtatgcaggc catatgccat cgcagctgct gtgggttcat
     1321 taattaagcg taccgcattt aacccggcta actccgcagc taaacgggta tgcttgcgtt
     1381 gttcatcgct gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa
     1441 attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa
     1501 atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcgcgttta aataacgctg
     1561 ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat
     1621 tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta
     1681 attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtgccg agatcgatac
     1741 caatggtgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag
     1801 attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa
     1861 tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt
     1921 ggcattcggt ttattgtcag tcagataata ataatcaaca accgtcatac cctgggtata
     1981 tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa
     2041 cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc
     2101 tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc
     2161 aatcgcgcgg aaacgctcgg tgtcttcaat gtggatttgc gctttatgag taacatccag
     2221 accggccatc accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac
     2281 gtaaatatta aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat
     2341 cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt
     2401 agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc
     2461 catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc
     2521 atccagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt
     2581 taccgcgccg cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac
     2641 attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc
     2701 aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca
     2761 atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg
     2821 taacgccagg atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact
     2881 tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc
     2941 cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc
     3001 cataaagatc acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat
     3061 catggtcatt ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc
     3121 cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaacgc
     3181 gcgagcgatt gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc
     3241 atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcgcttt
     3301 atcgcgttta agcactttca cctgcgccag agtcaggttt tcaataatcg acaaatgggg
     3361 gaacaactca aaatgctgga acaccatccc gacgcgggaa cgcagctttg ccagatcggt
     3421 tttcttgtca ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag
     3481 gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac
     3541 cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca
     3601 ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac
     3661 aacgacgcgc taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc
     3721 tgcgtaccat cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac
     3781 acatacacca gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc
     3841 atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc
     3901 aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc
     3961 tctgaatagt aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata
     4021 tcattttttg gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac
     4081 cacagcaaaa ccataactaa aggaatcgag cggaatatgt taacgtaggc tttggcaaac
     4141 cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata
     4201 ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat
     4261 ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc
     4321 caggcaggcg aactttacgt tcaaccagcg tcattaccag catgataatc gcgttaatca
     4381 acacatacgc cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca
     4441 atttacccgc ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt
     4501 tcaccaggtt catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca
     4561 gcagtacgta tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt
     4621 gcccacgcgg caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa
     4681 acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct
     4741 taaaccacat gccgattttc tccggcaaca gctccgggat caccagatac caggtaaaga
     4801 attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga
     4861 gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa
     4921 tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat
     4981 aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca
     5041 taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac
     5101 ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg
     5161 tgctacgtaa caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt
     5221 gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
     5281 ttcaggtttt tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca
     5341 ccggaagtct gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcatct
     5401 ttacgcaaca tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg
     5461 tctggtttct tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca
     5521 acggcacgac cgctttccag ggtgcggaaa gagtcaccgt gatctttggc gctgatgatg
     5581 cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg
     5641 gaagtgacga ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccgcccttt
     5701 ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg
     5761 cgttcgacgt tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt
     5821 ggaatacggt tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt
     5881 ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt
     5941 tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca
     6001 ccgtttttgg cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt
     6061 gccagtcctg cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata
     6121 tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt
     6181 gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct
     6241 aaatgtaaag atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga
     6301 agcaaaattc ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg
     6361 gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa
     6421 atacttaggg gcaaattaat tacgattgta attttaggct gaaaaagtca tatttcggac
     6481 agaattatct tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc
     6541 tttttacctg tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta
     6601 aatgttcaat tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt
     6661 tatctcgcac aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc
     6721 gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt
     6781 ctgcacagta gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc
     6841 gtggcggaac agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc
     6901 tttggtgggc tcatcagcgc ctgttatgcg ttacgcgata gtgatcagat agtcatcagc
     6961 gttggggcat ccggggcaat tatgggaata gctggcgctg cgatagcaac acagcttgct
     7021 tcaggtgcgg gcacatacca taaaaaccag cggcgagtat tttctctgtt gggtatggtg
     7081 gcgctgacac tgttgtatgg tgcccggcaa acaggaatag ataatgcttg tcacattggc
     7141 ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaaccga
     7201 ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg
     7261 cttgcgcagc agcagatgga tgagtcagtt ttacaagtca ggcaaagcct gcgtgaagcg
     7321 ttttatccac aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa
     7381 cgcaacgccc tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat
     7441 ttgctcgctg agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg
     7501 tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc
     7561 ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt
     7621 aaagatcagg gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact
     7681 tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg
     7741 attaccaccg gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg
     7801 gtgatgaatc gcaccagtaa tagtgtgtca gcgattgatc tggtgactta
//
LOCUS       DAJTYD010000173         7820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTYD010000173 DAJTYD010000000
VERSION     DAJTYD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.173"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            270..413
                     /locus_tag="OO585_003392"
     CDS             270..413
                     /locus_tag="OO585_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6630866.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            426..635
                     /gene="cspE"
                     /locus_tag="OO585_003393"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             426..635
                     /gene="cspE"
                     /locus_tag="OO585_003393"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6630867.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            801..1610
                     /gene="rlmA"
                     /locus_tag="OO585_003394"
     CDS             801..1610
                     /gene="rlmA"
                     /locus_tag="OO585_003394"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6630868.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OO585_003395"
     CDS             complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OO585_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6630869.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2601..3059)
                     /locus_tag="OO585_003396"
     CDS             complement(2601..3059)
                     /locus_tag="OO585_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6630870.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OO585_003397"
     CDS             complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OO585_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6630871.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OO585_003398"
     CDS             complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OO585_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6630872.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OO585_003399"
     CDS             complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OO585_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6630873.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6275..>7820
                     /gene="yoaE"
                     /locus_tag="OO585_003400"
     CDS             6275..>7820
                     /gene="yoaE"
                     /locus_tag="OO585_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6630874.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDVDNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1988 a         1914 c         1940 g         1978 t
ORIGIN      
        1 acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt
       61 agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc
      121 ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg
      181 tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat
      241 ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga
      301 gtaaacacgt tgatgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca
      361 gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt
      421 ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct
      481 tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct atccagggta
      541 atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga
      601 aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga
      661 atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc
      721 ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt
      781 agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag cctctttcgc
      841 gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt
      901 atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa
      961 tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa
     1021 ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct
     1081 gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg
     1141 gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt
     1201 tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac
     1261 gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg
     1321 tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg
     1381 aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta cagcagagtg
     1441 cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga
     1501 cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt
     1561 tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt
     1621 gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga atttccgctt
     1681 ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt gacataatca
     1741 aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac
     1801 caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga
     1861 aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa
     1921 tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga
     1981 caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg
     2041 caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac
     2101 ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag
     2161 cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag
     2221 caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca ggcatagcga
     2281 ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc
     2341 aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg
     2401 gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt atttcattgc
     2461 tttattgata acgaattcat ttagatttat atatcaatga aacgttaaaa taaataacaa
     2521 ataagggaag aggtaatata gcctaagcta tgtctgatta ttaaaaatac actaaacata
     2581 gctttggcta aattcataac ttattgagtt aaaacgataa gcttataaat tctttccaga
     2641 ttgtcgatat ttcgaacgcg gattaacagc cgacgctgtt ctaattgcat caccaacacg
     2701 ccatcttccg acaagttcat cgctttgatt cggctatatt caatccagac attggcgaag
     2761 aaaaaacctt tttgtttaaa gatgatcttc ggaacgcgga tccagaatat ataaaaaccc
     2821 atcagagcca gtgcgcttaa taaccatgtc gttattaatg caccatgatt cgtgacgttg
     2881 ttatagataa gaatgacaat caatccgacg aagataacgc tatcgatgcg accacgccgg
     2941 agcaaaggaa ttgccagcag ggtggggccg ttacggcggg gcatgatgaa ctgatcgtag
     3001 atcgcgaagg ccaggagtgc ggcgatgaaa agaatcagta ccaggtccgt gattgtcatt
     3061 aatcctccag ataaaaaaac ggggccaaaa ggccccggta gtgtacaaca gtcttacagt
     3121 cccagcaggc cgcaagcgta accagcgata ccgatgacga agaagccaac gatgatccac
     3181 agcgggttaa cttttttgcg cagtagccac atacaagcaa aggtcagcag cagtggtacc
     3241 aggcctggca ttaactggtc cagaatagtc tggacagtag taacgtgttc tttgcccgtc
     3301 tggtcagtaa tgcgagagac aaccagcggg atgttgacat gtgtccactt gttaaccaat
     3361 gcccccatga caaacaggcc gaggatagac gccccttccg tcagtttttg caggaagcca
     3421 ccacccatat ctttaacgat atcgatacct ttggagtaac catacgctac gccgtagtaa
     3481 cgggttgcca gacgcaccag gttaaacagg atgaagaaca gcagcggacc taacaggctg
     3541 ccgctcatcg cgataccggc acccagtgct gcaaataccg gacgtacggt tccccagaag
     3601 atcgggtcgc ctacaccagc cagcggcccc atcaaaccga ctttgatacc gttgatagca
     3661 ccgtcgtcga tctctgcgcc attagcacgc tgttcttcca gcgccagggt tacgccgaga
     3721 atcggcgcag ccacgaacgg ctgggtgtta aagaactcca ggtgacggcg aatagcttgt
     3781 ttacgagctt cgttgttctc agggtagagg cgacgaattg ccggtaccat agagaagcag
     3841 aaacccagtg cctgcatacg ttcgaagttc catgaaccct ggaagaggtt agaacgcagg
     3901 aagacgccac gaatatcact ttgagtgagt tttttctcgg tggtagtttg agttgtatca
     3961 accatttcgc tcacctgtta gtccagttcg ttatcgagat cgttgttacc agcagcctga
     4021 gcaggcgcac cggctacgcg gttgtatttc gggctaagtt ggatgtagag cactgccata
     4081 acagtaccga tcacacccag agcaaccagg ttaaagttgg tgaatgctgc ggttacgaag
     4141 ccgaggtaga agaacggcat caggtagcca gcacgcatca tgttgataac catcgcataa
     4201 ccaaccacca cgatcatgcc accggcgata ttcagaccat tggtcaccac ttccggaatc
     4261 gcattcagca tgttctgtac ttcgctggta ccaacagaca gcgcaacgat gacggccgga
     4321 atagccacac gcattgcttg caggaacaga gaagaaacgt ggatccagga aatcgctgtc
     4381 aggttgccgt tatcagcagc cttatccgca gcgtgctgga aagcaacggt aatagtacga
     4441 acgatgatgg tcagtacctg gcccgcagcg gccagaggga ttgccagtgc gatacctgca
     4501 ccaatgctct gatgacctgc gataaccaga atggtagaaa tgatagaagc cagagcggcg
     4561 tcaggcgcaa ctgcagcacc gatgttcatc cagcccagcg cgatcatttc cagcgtacca
     4621 ccgataataa taccggtttt catatcccca agaacgatac ccaccagggt acacgcgatt
     4681 agcggacggt gaaactgaaa ttcatcgagg attgatccca tacctgcgat acaggctacg
     4741 ataaatacca gcacaatttg aagagtggta atctccattg tacttctcct gtttacgtaa
     4801 gacttaagtg tgaaaactga gtgataatca acacaatacg ttacttatcg attttgctga
     4861 tcagatccat cattttcagt ttcggatcgg tggaaacctt acggacttcc agctcaatac
     4921 cgcgcgcatt cagtttcttg aacgcctcga tatctttttc atcaaccgaa accgcgttat
     4981 tcacctgggt tttaccctga cggaatgcca taccaccgac gttaacagag gtgattttca
     5041 cgccgccttc aacgagacgc tctacatctg ttgggttggt aaataacagc attacgcgtt
     5101 cgccagcata tttcgggttg ttgtagacgc gaatcatttt ggcaacatca actacgtgtg
     5161 ctgttacgcc cggaggtgca acctgggtga gcagtgtctt acgaacggta tccgcagcca
     5221 cttcatcact aacaacaata atacgggaga cattggtttc tttggtccag cgggtggcga
     5281 cctgaccgtg aatcagacgg tcgtcgatac gcgcaaggcc aataaccatg tagtcgtttg
     5341 gccccattgg ttttgccgga gttggagccg cttttggtgc tgcggcagcg ggtgctggcg
     5401 cggctttttc aaccggtttg gctttcagtg ctttcacacc ttcacggcct gtttctactg
     5461 ccagcgcaac cagttcatca aagcttgggt catcatcacg agccattaac gtttccacga
     5521 gcattggaat gttaacgcct gcaatgactt cataatgctc tttgtcgacg acaatgcggc
     5581 tggcagcatt gaacgggctg cctccccatg tatcaacgag aaacagcacg cctttagtgg
     5641 tgtcgagttt tgccaactga gcgttgtact tttcaatcag cgtttcggca ttttcacctg
     5701 gaacgaaatc gatccagccg acgttttcct gctcgcctaa cagcatttct gccgttttaa
     5761 gcaactgctc tgcagcccaa ccatgtgtgc ctataacaat agcaatggtc acttgctacc
     5821 tcctttatta tcgttaacac ctcaacgtgc cagatgtatt tttgaatcgt cgtccacaat
     5881 cgaatcgatt cagataaggg ctacaaccaa aaacccctgc gtttcgcgat ttattttaga
     5941 tatcgaaaaa attattttat gtgatgaaga tccgtaattt aactttcgat tagcagaaat
     6001 ttcgaaaggt aaaatatcct tgtcacattc gtttgcaaag gaaggtaaat ctttgccaaa
     6061 tcagaggcgt ctctgatatg tttaactccc gtttaacaac catggagtat agggcagtag
     6121 cccgcagtat ggatcgtcac cgacgtcatt tcagcatcag gccttttaac gcctgccttt
     6181 ctggcactct atgccgcacc tttcgtttgc attttgtcgt tacgcctgca ttatttctgg
     6241 cgtcgaatag ctattcctta agcaggagct tgtcatggaa ttcttaatgg acccctcaat
     6301 ttgggcgggg ctactcacgc ttgttgttct cgaaattgtg ctgggtatcg ataacctggt
     6361 cttcatcgcc attcttgctg acaaactgcc gccaaaacaa cgcgataaag cgcgtttgct
     6421 ggggttatca ctggcgctga ttatgcgtct ggggctgctg tcgctgattt catggatggt
     6481 cacgctgacc aaaccgctat ttaccgtcat ggatttctcc ttctccggac gcgacctgat
     6541 tatgttgttc ggggggatat tcttgctgtt caaagcaaca accgaactgc atgaacggct
     6601 ggaaaaccgc gatcatgatt ccggccacgg taaaggctac gccagtttct gggtggtcgt
     6661 cacacagatt gtcatccttg acgccgtctt ctcgttggat gcggtaatta ctgcagtagg
     6721 gatggttaac catctgccgg tgatgatggc ggcggtagtg attgcgatgg cggttatgtt
     6781 gctggcatcc aaaccgctga cgcgattcgt taaccagcac cccacggtgg tggtgctctg
     6841 tctgagcttc ctgttaatga ttggtcttag tctggtggca gaaggtttcg gtttccacat
     6901 tccgaaaggt tacctgtatg ccgcgattgg cttctcgatc atcatcgaag tgtttaacca
     6961 gattgcgcgt cgcaacttta ttcgccacca gtcgactttg ccgctgcgag cgcgtactgc
     7021 cgatgccatc ctgcgtttga tgggcgggaa acgtcaggcc aatgttcagc acgatgtcga
     7081 taacccgatg ccgatcccgg aaggtgcatt tgccgaagaa gaacgttaca tgattaacgg
     7141 cgtactgacg ctggcgtcgc gttctctgcg cgggatcatg acgccgcgcg gtgaaataag
     7201 ctgggttgac gctaatctcg gggtcgatga aatccgcgag caactgctct cttcaccgca
     7261 cagtctgttc ccggtatgtc gcggtgaact ggatgaaatc atcggtattg tacgtgctaa
     7321 agaactgctg gtggcgctgg aagagggcgt tgatgtggcg gcgattgctt cggcgtctcc
     7381 ggcgattatc gtcccggaaa ccctcgatcc gatcaacctg ttgggcgtgc tgcgtcgtgc
     7441 tcgcgggagc tttgttatcg tgaccaacga gtttggtgtg gtacaaggtc tggtcacgcc
     7501 gctggatgtg ctggaagcca ttgcgggtga attcccggac gctgacgaaa cgccggaaat
     7561 cattactgat ggtgacggct ggctggtaaa aggcggtaca gatttgcatg ccttgcagca
     7621 ggcgcttgat gttgagcacc ttgccgatga cgatgatatc gcgacggtcg cgggcctcgt
     7681 gatctcggca aatggtcaca ttccccgtgt gggcgatgtg attgatgtag ggccactgca
     7741 tatcaccatc attgaagcca atgattatcg tgttgatctg gttcgcattg ttaaagagca
     7801 accggcgcac gatgaagatg
//
LOCUS       DAJTYD010000174         7753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTYD010000174 DAJTYD010000000
VERSION     DAJTYD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.174"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..997
                     /gene="ruvB"
                     /locus_tag="OO585_003401"
     CDS             <1..997
                     /gene="ruvB"
                     /locus_tag="OO585_003401"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6630875.1"
                     /translation="RLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAK
                     LRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLTNLEPH
                     DVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAG
                     SLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIA
                     NRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPV
                     GLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPEMP"
     gene            complement(1047..1832)
                     /gene="znuB"
                     /locus_tag="OO585_003402"
     CDS             complement(1047..1832)
                     /gene="znuB"
                     /locus_tag="OO585_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6630876.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(1829..2584)
                     /gene="znuC"
                     /locus_tag="OO585_003403"
     CDS             complement(1829..2584)
                     /gene="znuC"
                     /locus_tag="OO585_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6630877.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            2663..3595
                     /gene="znuA"
                     /locus_tag="OO585_003404"
     CDS             2663..3595
                     /gene="znuA"
                     /locus_tag="OO585_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6630878.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            3611..4933
                     /gene="mepM"
                     /locus_tag="OO585_003405"
     CDS             3611..4933
                     /gene="mepM"
                     /locus_tag="OO585_003405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6630879.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5053..6024
                     /gene="lpxM"
                     /locus_tag="OO585_003406"
                     /gene_synonym="msbB"
     CDS             5053..6024
                     /gene="lpxM"
                     /locus_tag="OO585_003406"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6630880.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(6113..>7753)
                     /locus_tag="OO585_003407"
     CDS             complement(6113..>7753)
                     /locus_tag="OO585_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6630881.1"
                     /translation="IDGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTS
                     ESATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCIS
                     RVYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININ
                     NNWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGK
                     EYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIY
                     HYNSSYLRFAFTLTQDSPLVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVID
                     RPTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLKLTLLRSQHK
                     PGEVVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPV
                     KPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYH
                     YVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2105 a         1793 c         1902 g         1953 t
ORIGIN      
        1 ccgtctgatt tctgccggta ccactttgcc ggaagatgta gcagatcgcg ccattcgccc
       61 taaattactg gaagagtatg ttggtcagcc gcaggttcgt tcacagatgg agattttcat
      121 caaagcagcg aaactgcgcg gcgatgccct cgatcatttg ttgatttttg gtcctccggg
      181 gttgggtaaa actacgcttg ccaacattgt cgccaatgaa atgggcgtta atttacgcac
      241 gacttctggt ccggtgctgg aaaaggcggg cgatctggca gcgatgctca ctaaccttga
      301 accgcatgac gtgctgttta ttgatgagat ccaccgttta tcgccagttg ttgaagaagt
      361 gctgtacccg gcaatggaag actaccaact ggatatcatg attggtgaag gtccggcggc
      421 acgctccatt aaaattgatt tgccgccgtt taccctgatt ggcgcaacca cgcgcgcagg
      481 ttcgctgaca tcaccgttgc gcgaccgttt tggtattgtg caacgtctgg agttttatca
      541 ggtgccggat ctgcaatata tcgttagtcg cagtgcacgc tttatggggc ttgagatgag
      601 tgatgacggc gcgctggaag ttgctcgtcg cgctcgcggt acgccacgta ttgccaaccg
      661 tctgctgcgt cgagtgcgtg atttcgccga agtgaagcac gatggcacca tctcggcaga
      721 tatcgctgct caggcgctgg acatgttgaa tgtcgatgct gaaggtttcg attatatgga
      781 ccgcaaattg ttgctggcgg taatcgataa gttctttggt gggccggtag gtctggataa
      841 cctggcggcg gctattggcg aagaacgtga aaccattgag gatgtgctgg aaccttattt
      901 gattcagcaa ggctttttgc agcgtacacc gcgtgggcgt atggcgacga cgcgggcgtg
      961 gaatcacttt ggcataacgc cgccagaaat gccgtaagtc ggattggtcg ctcgcgccgc
     1021 atccgacaaa tgtgttcagc gatagattag ctggcctgct ttttcatcat actgataata
     1081 aataacagtg ctgcacatag caccaccgac ggacccgccg gcgtatcgta aaatgcggaa
     1141 aaggttaaac cgcccgttac ggcaaccatc cccaccaaca cagcgacacc tgccatctgt
     1201 tccggcgtgc gggcaaagcg acgtgcagta gcagcaggaa taatcagcag tgaagtaata
     1261 atcaacgcgc caacaaattt catcgctaca ccaatcgtca atgccgtcac cagcatcaac
     1321 aacaatttca cgcgctgtaa tttcacacca tcaacaaacg ccagatccgg gctaatcgtc
     1381 atcgacagca aattgcgcca ttgccagaac aaaatagcca ccacgatgac cacgccaatc
     1441 gcaatagcga tgagatcttc tggcgtcact gccagcaaat caccgaacag gtaagccatc
     1501 aaatcaacac gaatgttaga catcagacta acgaccacca ggcccagcga cagggcactg
     1561 tgcgccataa tccctaataa cgtgtcaagc gccagctgtg gacgtttctc cagccatacc
     1621 agaccgcccg ccaggagcag cgtaacggca attaccgcat agaatggatt cacgtccagc
     1681 aacaaaccaa acgcgacgcc aagtaatgag gcatgagcca gcgtatcacc gaaataagac
     1741 atacgacgcc agactacaaa cgaacccagc ggacccgcgg cacaggcgag catgatcccg
     1801 gctaaccaac cgggaaataa taattcaatc atgagcgatc atttccccga cgcaaaacaa
     1861 ttcgtccctg taaatcgtga cgatgattat gatgatggcg atagataccc agttgttcag
     1921 caccacgagg accaaacata gaaataaact ccggatgtag ggaaacaact tccggtgtgc
     1981 cggaacaaca aatgtggtga ttcagacaca gcacttcatc ggtttttgcc attaccagat
     2041 gcagatcgtg agagaccatt aaaacgccac aatccagttc gcgacgcaac tggtcaataa
     2101 ggtcatataa cgccacctgg ccatttacat ccacgccttg agtgggttca tccagcacta
     2161 ataattgcgg tcgatttaac aatgctcgcg ctaacagtac acgctgtgtt tcgccaccag
     2221 agagcttttg catcggtgcg ttaatcagat gcccggcctg gacacgtttc agtgcaggca
     2281 aaatatcttc tttatgtgtg ccagggcgta agcgtaaaaa acggtttacg gtcagtggca
     2341 acgtggtgtc gagatacagc ttctgcggta catagccgat gcgtagtttt ccgttgcgct
     2401 tgataacccc ttcatcgggt gttaccagcc cgagcactac ccgtaccagt gtcgacttac
     2461 ctgcgccatt tggcccaagt aaagtcaaaa tttttccagg tttaagttcc agcgacacat
     2521 cagagaggac gcggcgttgg ccaaaagaaa ccgagacatt ttccagggaa accagacttg
     2581 tcatgttaat tttagtcttg cagtagtcat gagatgttat aatatcacac ttctcatatt
     2641 cattacgatg attggtcgca ttatgttaca taaaaaaacg cttcttttcg cagcattatc
     2701 cgccgctctc tggggaggtg caacacaggc cgcagatgcc gccgttgtcg cttcgcttaa
     2761 acccgttggg ttcatcgctt ctgccattgc tgatggtgta acagaaacgg aggttttact
     2821 gcctgacggc gcttcagaac atgattattc actgcgtcca tcggatgtaa aacgcttaca
     2881 gaacgcggac ttagtcgttt gggttggccc ggagatggaa gcgtttatgc aaaaaccggt
     2941 aagcaaatta ctaggagcga agcaggtaac gattgcgcag cttgaagatg tgaaactgct
     3001 actgatgaaa agtattcacg gcgatgatga tgatcacgac cacgcggaaa aaagtgaaga
     3061 agatcaccat cacggcgatt tcaacatgca tctttggctt tccccagaga tagcgcgggc
     3121 tacagcggtt gcaatccatg gaaaattagt ggaacttatg ccgcaaagtc gagccaaact
     3181 tgacgccaac ctgaaggatt ttgaggcaca attagcctca accgaaacgc aggttggtaa
     3241 cgagctcgcg ccgctcaagg ggaaaggtta tttcgttttt cacgatgctt acggctattt
     3301 cgaaaaacag ttcggactga caccgcttgg tcattttacc gttaaccctg agattcaacc
     3361 tggcgcgcag cgtttacatg aaataagaac acagttggtt gagcaaaaag caacctgcgt
     3421 ttttgctgag ccacagttca ggccagcggt cgttgagagc gtcgcacgag ggacatccgt
     3481 tcgtatggga acgttggatc ccttggggac gaatatcaaa ctgggtaaaa caagctattc
     3541 agaattcctg agtcaattag ccaaccagta tgcgagctgc ctgaaaggag attaatgagg
     3601 aagtgattac gtgcaacaga tagcccgctc tgtcgccctg gcgtttaata atttaccgcg
     3661 accacaccgc gttatgttgg ggtcgctcac cgttcttact ctggccgtcg ctgtctggcg
     3721 gccctatgtt tatcaccgcg atgccacgcc aattgtcaaa accattgagc tggaacagaa
     3781 cgaaattcgt tcgctcttac ctgaagccag tgagccgatt gatcaagctg cacaagaaga
     3841 tgaagccatt ccccaggacg aactggatga caaaatcgcc ggtgaagcgg gcgtgcatga
     3901 atatgttgtt tccactggcg atacgctaag cagcattctc aatcagtatg gtattgatat
     3961 gggtgatatc acccaactgg ctgcggccga caaagaattg cgtaacctga aaatcggtca
     4021 acaactctcc tggacattaa ccgcggacgg cgaactgcag cgcctcacct gggaagtgtc
     4081 tcgtcgtgaa acccgaacct atgaccgtac tgccgctaac ggttttaaaa tgaccagcga
     4141 aatgcagcaa ggagagtggg ttaacaatct gctgaaaggt accgtcggag ggagctttgt
     4201 tgccagcgcc agaaacgccg gtttaaccag cgccgaagtg agcgcagtga ttaaagccat
     4261 gcagtggcaa atggatttcc gcaaactgaa aaaaggcgat gaatttgcgg tgttaatgtc
     4321 tcgagagatg cttgatggta aacgtgagca aagccagctg ctgggcgtac gtttgcgttc
     4381 agaaggtaaa gattattacg caatccgcgc tgaggatggc aaattctacg accgtaacgg
     4441 tactggtctg gcgaaaggat tcttgcgatt cccaacggcg aaacagttcc gtatctcgtc
     4501 taactttaac ccgcgtcgta ctaatccggt gaccggtcgc gttgcaccac acagaggtgt
     4561 tgatttcgcc atgccgcagg gtacgccagt gctttcagtg ggtgacggtg aagtggtggt
     4621 tgccaaacgc agtggcgcag caggttatta tgtggctatt cgtcatggtc gcagctacac
     4681 cacgcgttat atgcacttgc gcaagattct ggtgaaaccg ggacagaagg tgaaacgtgg
     4741 cgaccgtatc gcgctttccg gtaataccgg acgttcaacc gggccgcatc tgcactatga
     4801 agtatggata aaccagcagg ccgtaaaccc gctgacggca aaactgccgc gtaccgaagg
     4861 gctgaccggc tccgatcgtc gcgaattcct ggcgcaggcc aaagagattg tgccgcagct
     4921 acggtttgat taattaacat ccattcgcag ccggtacgca gtcagtaccg gcttttttta
     4981 tttggtgcgg ggcaagttgc gccgctacac tatcaccaga ttgatttttg ccttatccga
     5041 aactggaaaa gcatggaaac gaaaaaaaat aatagcgaat acattcctga gtttgataaa
     5101 tcctttcgcc acccgcgcta ctggggagca tggctgggcg tagcagcgat ggtgggtatc
     5161 gctttaacgc cgccaaagtt ccgtgatccc attctggcac ggctgggacg ttttgccgga
     5221 cgactgggaa aaagctcacg ccgtcgtgcg ttaatcaatc tgtcgctctg ctttccagaa
     5281 cgtagtgaag ctgaacgcga agcgattgtt gatgagatgt ttgccaccgc gccgcaagcg
     5341 atggcaatga tggctgagtt ggcaatacgc ggaccggaga aaattcagcc gcgcgttgac
     5401 tggcaagggc tgcagatcat cgaagagatg aggcgtaata acgagaaagt tatctttctg
     5461 gtgccgcacg gttgggccgt cgatattcct gccatgctga tggcctcgca agggcagaaa
     5521 atggcagcga tgttccataa tcagggcaac ccggtttttg attatgtctg gaacacggtg
     5581 cgtcgtcgct ttggcggtcg tctgcatgcg agaaatgacg gtattaaacc attcatccag
     5641 tcggtacgtc aggggtactg gggatattat ttacctgatc aggatcatgg cccagagcac
     5701 agcgaatttg tggatttctt tgccacctat aaagcgacgt tgcccgcgat tggtcgtttg
     5761 atgaaagtgt gccgtgcgcg cgttgtaccg ctgtttccga tttatgatgg caagacgcat
     5821 cgtctgacga ttcaggtgcg cccaccgatg gatgatctgt tagaggcgga tgatcatacg
     5881 attgcgcggc ggatgaatga agaagtcgag atttttgttg gtccgcgacc agaacaatac
     5941 acctggatac taaaattgct gaaaactcgc agaccgggcg aaatccaacc gtataagcgt
     6001 aaagatcttt atcctatcaa ataaaaaaaa gcctctcaaa ttattgagag gctgtactcg
     6061 ataaaaaatc tcaatgagtg ttcccactat tttgagtttt taggttagta cgctattgag
     6121 ttaatcgtag cgtgataatt tcaaaaggtt tgaactcaag attgcaatat tgattgcaat
     6181 aaaaactgtc atctaactta ttttcttgaa gatcgacata ttgaacgtag tgataattac
     6241 atacccacga aagcatggct gaaatttcta atccatgact ctcatataat cttacgatta
     6301 taccgtcgcc gtcttcagct aattttattg tttcgatgat aacgctgtta gtatcgatag
     6361 caaaaaatga tagtgaaggg ggtaattcac ccgggtgcgg tttgacaggg acgacctgta
     6421 agggatgatt cattatatag gcttgattaa taacaccacc cacacggtaa tctccatgat
     6481 gaggaaataa ggagaatatg aatttttgtt taccaatatc tgcaaaatta ttaatcagga
     6541 aattatcata attgtctgtc ttaacaactt ccccaggttt atgctgcgaa cgcagcaaag
     6601 ttaatttaag cgtctggtct ttgacacgat agccatattt gttttttgct attaaagcta
     6661 tgccagtttc tcgatcagaa aggtcgaccc agtgatgtgc tggtatttca tctttggcaa
     6721 aagcgaacga atcatcgcta tgcgttggtc tgtcaattac accgaactga atttgacatt
     6781 gtgcaagaga tgtttgtact gacaccggga atttgacttt gagagaaaca ttaggttcgt
     6841 gccaatcaat aaaagtttgg acatcaacga gtggactgtc ctgtgtcagg gtaaatgcga
     6901 agcgaagata agaggaattg tagtgataaa tcagcgtcat cgtagtttta ggcccgctga
     6961 ttacagttgt cattttttca gcgagtaaaa catatgattc cccatcacga taatttgaag
     7021 caaaatccca ggccgcgaaa aaacctgcat cttcgtggta tgcacggatc tcactgtgca
     7081 tattttcagt aatgtattct ttaccatatc ttttatcata tagcgaaatt aaatcaccgt
     7141 tttctgaaaa aagaagttta atgtaattat tttcaatact tcgagattct gccttcagac
     7201 catttacgat cttattcttt ggatcaatca cagcataacc ataagagttt actcgtgctt
     7261 tgagccagtt gttgtttata ttgatccatt cgttacgagc aaaacaggtg gtattaaata
     7321 aaatgtgcgg atctttataa gaagaagtat caattttact taataaagta gactgcgtat
     7381 cagaaataat tttctcggtt tttgcttcta gtttaatata ttccttttca gtttcaagat
     7441 aaactcttga aatacatgat ccgggaagga tgtcatggaa ttgcaaggtc agcatctctt
     7501 tccatatttc atcaaactca ctgcgaagaa ttgatgtcat attattagta atagttgtaa
     7561 aaaattcagc atcatgtaat ttattttcca tatttcgatt atgagcttta gttgctgact
     7621 ctgatgtaaa gcacccttga tgagcctcga aataaagttc acctgaaata atgggcaagc
     7681 tttcgcgaaa agcgtcggcg taagtgaaga acttatcaac acggctgaaa tcaacatgag
     7741 gtaagccatc aat
//
LOCUS       DAJTYD010000175         7699 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTYD010000175 DAJTYD010000000
VERSION     DAJTYD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.175"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..267
                     /locus_tag="OO585_003408"
     CDS             <1..267
                     /locus_tag="OO585_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6630882.1"
                     /translation="FPFIAIAGSTAQGGVIHFYGQIVEPACDVSTQSSPVEMNCPQNG
                     SVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEYN"
     gene            363..896
                     /gene="ymdB"
                     /locus_tag="OO585_003409"
     CDS             363..896
                     /gene="ymdB"
                     /locus_tag="OO585_003409"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6630883.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            838..2319
                     /gene="clsC"
                     /locus_tag="OO585_003410"
     CDS             838..2319
                     /gene="clsC"
                     /locus_tag="OO585_003410"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6630884.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLVAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2327..3484)
                     /gene="mdoC"
                     /locus_tag="OO585_003411"
     CDS             complement(2327..3484)
                     /gene="mdoC"
                     /locus_tag="OO585_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6630885.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            3878..5413
                     /gene="mdoG"
                     /locus_tag="OO585_003412"
     CDS             3878..5413
                     /gene="mdoG"
                     /locus_tag="OO585_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6630886.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5406..>7699
                     /gene="mdoH"
                     /locus_tag="OO585_003413"
     CDS             5406..>7699
                     /gene="mdoH"
                     /locus_tag="OO585_003413"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6630887.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMAT"
BASE COUNT         1928 a         1831 c         2027 g         1913 t
ORIGIN      
        1 tttcctttta ttgccatcgc aggaagcacc gcgcaagggg gcgtgatcca tttttatggc
       61 caaattgtgg aaccggcatg tgacgtcagc acccagtcat cacccgtaga aatgaactgc
      121 ccacagaatg gttctgttcc gggaaaaacc tattccagca aagcgttaat gagcggcaat
      181 gtcaaaaacg cgcaagtagc ctcagtaaaa gtgcagtata ttgataaaca gaaaaagctg
      241 gctgtaatga acatcgaata taactaagtt tctgaacaac gcacgttgct gagtagaaaa
      301 atgcgattta accaaaaagc ctgctgtaca cttaagaaac aagaaggtgt taaggaggcg
      361 ttatgaaaac gcgtattcat gttgtgcagg gtgatattac caaactggcc gttgatgtga
      421 ttgtgaatgc ggctaatccg tcattaatgg gaggcggcgg cgtcgatggg gccattcatc
      481 gcgcagcggg tccggccctg ctggatgcct gtttaaaagt caggcaacag cagggcgatt
      541 gccctacggg gcatgccgtt attacgcttg caggcgatct tcccgctaaa gccgtagtac
      601 acaccgtcgg gccagtctgg cgtggtggtg aacaaaacga agaccagctt ttgcaggatg
      661 cctatctcaa tagtctgcga ctggtggcgg caaacagcta tacgtcagtg gcatttcctg
      721 caatcagtac tggggtttat ggttaccctc gtgcggcagc ggctgaaatc gcagtaaaaa
      781 ccgtttcaga atttattacc cgtcacgctt tacccgaaca ggtatacttt gtctgttatg
      841 atgaagaaaa cgcccacctc tacgaaagac tccttaccca acaaggagat gaatgatttg
      901 ccccggctgg cgagcgcggt gctgccactg tgttcgcaac atcccggtca gtgtggtctt
      961 ttccctctgg agaaaagtct ggatgcgttt gccgcccggt atcgtctggc cgaaatggca
     1021 gagcatacgc tggatgttca gtattacatc tggcaggacg atatgtcggg tcggttactg
     1081 ttttccgccc tgttagtcgc agcaaagcgt ggcgttcgcg tccgtttgtt gctggacgac
     1141 aacaatacgc ccggacttga cgatatttta cgcttgcttg acagccatcc ccgcattgaa
     1201 gtccggcttt ttaatccttt ctcgtttcgc ttgctgcgtc cgcttggtta tatcaccgac
     1261 ttttcccgtc tcaatcgccg tatgcacaat aaaagtttca ctgtcgatgg cgtggttacc
     1321 ttggtgggag gacgaaatat tggtgatgcc tattttgggg caggggaaga gccacttttt
     1381 tcggatttag atgttatggc aataggaccc gtggtagagg acgttgccga tgatttcgcc
     1441 cgctactggt attgcaaatc ggtttcaccc ttacagcagg tgctggatgt cccggaaggt
     1501 gaaatggcgg atcgcatcga gttacccgcc tcctggcata acgatgccat gacgcatcgt
     1561 tacttacgcc aaatggaatc cagtccgttt ataaatcatc tggttgatgg aacgttgccg
     1621 cttatctggg cgaaaacacg tttattaagt gatgatccgg cgaaagggga gggcaaggca
     1681 aaacggcatt cactgttacc gcagcgcctg ttcgatatca tgggctcacc cagtgaacgc
     1741 atcgatatta tctcttccta ttttgtaccg acacgcgcag gtgtggcgca actcttacgg
     1801 atggtgagaa aaggggtaaa gattgcgatc ctaaccaatt ctcttgccgc taacgatgtt
     1861 gctgtcgtcc atgccggata cgcgcgctgg cgcaaaaaat tgctccgcta tggcgtggaa
     1921 ttatatgaac tcaagccgac gcgtgaacaa agtagtacgt tacacgatcg cggcataacc
     1981 ggtaattccg gtgccagcct gcatgctaaa acctttagca tcgatggtaa aacggtgttt
     2041 atcggctctt tcaatttcga tccacgttcg acattgctca atactgaaat gggcttcgtg
     2101 atagagagcg aaacgctggc acagttaatt gataaacgct ttatacagag ccagtatgat
     2161 gcggcctggc agctccgtct ggacaggtgg ggacggatca actgggttga tcgtcatgca
     2221 aagaaagaga ttattctcaa aaaagaaccc gccaccagtt tctggaagcg ggttatggtc
     2281 agactggcgt cgatattgcc cgtggaatgg ttattgtaaa tgtggcttaa cgggctggtg
     2341 ctttatcgtt ctcacgctta acaaccggtt taccagaaaa caaaaacttc agtaacggga
     2401 tgcgtaaatg aatttcatac agaattatcg caatccctac tacgaatatc aggccacaga
     2461 gaaaaccaag ccagttggag gtgatgtgcg gtgtaatgta tgcgccgaaa aacagcgtta
     2521 acgggtggtg aaccagatag ataaatagcg atgcgttaac aaagtaagtc acccgcgctg
     2581 actggaagtt aagcaaacgg tggccaaagg agaagaccac attcaccatc cacagaccga
     2641 ggaccatggt gatcaccgac tcggtttcgt acatccaggc atcgccactg ccatagcgtt
     2701 ggttgagtaa ataagcgaca aatgccaatg ctgctgcaag ggtacagcca cgagacggcg
     2761 tggtaaacaa ggctttaaga tgagggaaaa tgaaagccag tgcaccgagg ataaagaacg
     2821 gcaaataaaa tagcgtttgc atgacaataa aattgaacat gccattactc agaatgggcg
     2881 gatacacaat aaaaatcgtt cttcttatta ccgcatagcc gatgccgagg cataaaaaaa
     2941 tcaccgatag ttttaccatc gagaattttt tattcgtttt atcagaattt tctaaattat
     3001 ttctgatgcg tttaaatatc catacgcaca gcgtcgtcat gactactaac accagtaaaa
     3061 accacaggtg tgatattaat tcccaggcca acgtattata tttgtcatac aatgacagcc
     3121 caggccaact ttccgctttc cctttgacgt attgcagcat aataaattgc ggcaatgtca
     3181 gtaaggggat ggctgttaac atcgggatac ctacacgttc gacacgtact ttccaccatt
     3241 ttttcaaggg atagcgtaaa aaaagcatgt aggaaaagta gccggatata acgaaaaata
     3301 ccagcatgcg gaacgagtgg atgaagtcat taaaaagggt cagccacaat gacggttcgg
     3361 cgctattcac atgccatgta tggctcgaat agattaaaga aatatgaaaa gggatcccta
     3421 acaacatcag ccaggcgcgg atggagtcga ggaaatattc acgttgcgcg ggtactggat
     3481 tcatatatgg ttaactaatc tcggattttt cgtcttatcc ctgtcgggtt atgcctttag
     3541 gcttgttgcc atagcgacac cgacctgacc gcgccaggcg caggcttcaa ggtttttatg
     3601 catagcataa tcgctaccac taaccagaat ggaagcgtct gtaagacggt tgataaataa
     3661 aattgctggc aaaccctaca cgaagtcgat gcttctgtct ttaggagaag cacggaaagt
     3721 gaaaacggtt gcaatcaggt gcttaatcca tgagccagcg tgctgaacga taccgggatt
     3781 ctgttgtcgg aatggctggt tatccattaa aatagatcgg atcgatataa gcacacaaag
     3841 ggggaagtgc ttactaatta tgaaacataa actacaaatg atgaaaatgc gttggttgag
     3901 tgctgcagta atgttaaccc tgtatacatc ttcaagctgg gctttcagta ttgatgatgt
     3961 cgcaaagcaa gctcaatcct tagccgggaa aggctatgag gcgcccaaaa gcaacttgcc
     4021 ctccgttttc cgcgatatga aatacgcgga ctatcagcag atccagttta atcatgacaa
     4081 agcgtactgg aacaatctga agaccccatt caaactcgag ttctaccatc agggtatgta
     4141 cttcgatacc ccggtcaaaa taaatgaagt gactgccacc gcagtcaaac gaatcaaata
     4201 cagcccggat tatttcactt tcggcgatgt tcagcatgac aaagacacgg taaaagacct
     4261 tggttttgcc ggttttaaag tgctttaccc gatcaacagc aaagataaaa acgatgaaat
     4321 cgtcagcatg ctcggggcca gctatttccg cgtgattggt gcaggtcagg tttatggcct
     4381 ttctgcccgc ggcctggcaa ttgataccgc cttgccatcg ggtgaagaat ttccacgctt
     4441 caaagagttc tggatcgagc gtccaaaacc gactgataaa cgtttaacca tttatgcatt
     4501 gcttgactcg ccgcgcgcga caggtgctta caaattcgtg gttatgccag ggcgtgacac
     4561 ggttgtggat gtgcagtcga aaatctatct gcgcgataaa gtcggcaaac tggggggtgc
     4621 accgttaacc agtatgttcc tgtttgggcc gaaccaaccg tcgcctgcaa ataactatcg
     4681 tccggagttg cacgactcta acggtctctc tatccatgcc ggtaatggcg aatggatctg
     4741 gcgtccgttg aataacccga aacatttagc ggttagcagc ttctcgatgg agaacccgca
     4801 aggcttcggt ctgttgcagc gcggtcgtga tttctcccgc tttgaagatc tcgatgatcg
     4861 ttacgatctt cgtccaagcg catgggtgac tccgaaaggg gagtggggta aaggcagcgt
     4921 tgagctggtg gaaattccaa ccaacgatga aaccaacgat aacatcgtcg cttactggac
     4981 gccggatcag ctgccggagc cgggtaaaga gatgaacttt aaatacacca tcaccttcag
     5041 ccgtgatgaa gacaaactgc atgcgccaga taacgcatgg gtgcaacaaa cgcgtcgttc
     5101 aacgggggat gtgaagcagt cgaacctgat tcgccagcct gacggtacta tcgcctttgt
     5161 ggtcgatttt accggcgcag agatgaaaaa actgccagag gataccccgg tcacagcgca
     5221 aaccagcatt ggtgataatg gtgagatagt tgaaagcacg gtgcgctata acccggttac
     5281 caaaggctgg cgtctggtga tgcgtgtgaa agtgaaagat gccaagaaaa ccactgaaat
     5341 gcgtgctgcg ctggtgaatg ccgatcagac gttgagtgaa acctggagct accagttacc
     5401 tgccaatgaa taagacaact gagtacattg acgcaatgcc catcgccgca agcgagaaag
     5461 cggcattgcc gaagactgat atccgcgccg tacatcaggc gctggatgcc gaacaccgca
     5521 cctgggcgcg ggaggatgat tccccgcaag gctcggtaaa ggcgcgtctg gaacaagcct
     5581 ggccagattc acttgctgat ggacagttaa ttaaagacga cgaagggcgc gatcagctga
     5641 aggcgatgcc agaagcaaaa cgctcctcga tgtttcccga cccgtggcgt accaacccgg
     5701 taggccgttt ctgggatcgc ctgcgtggac gcgatgtcac gccgcgctat ctggctcgtt
     5761 tgaccaaaga agagcaggag agcgagcaaa agtggcgtac cgtcggtacc atccgccgtt
     5821 acattctgtt gatcctgacg ctcgcgcaaa ctgtcgtcgc gacctggtat atgaagacca
     5881 ttcttcctta tcagggttgg gcgctgatta atcctatgga tatggttggt caggatttgt
     5941 gggtttcctt tatgcagctt ctgccttata tgctgcaaac cggtatcctg atcctctttg
     6001 cggtactgtt ctgttgggtg tccgccggat tctggacggc gttaatgggc ttcctgcaac
     6061 tgcttattgg tcgcgataaa tacagtatat ctgcgtcaac agttggcgat gaaccattaa
     6121 acccggagca tcgcacggcg ttgatcatgc ctatctgtaa cgaagacgtg aaccgtgttt
     6181 ttgctggcct gcgtgcaacg tgggaatcag taaaagccac cgggaatgcc aaacactttg
     6241 atgtctacat tcttagtgac agttataacc cggatatctg cgtcgcagag caaaaagcct
     6301 ggatggagct tatcgctgaa gtcggtggcg aaggtcagat tttctatcgc cgccgccgtc
     6361 gccgcgtgaa gcgtaaaagc ggtaatatcg atgacttctg ccgtcgctgg ggcagccagt
     6421 acagctacat ggtggtgctg gatgctgact cggtaatgac cggtgattgt ttgtgcgggc
     6481 tggtgcgcct gatggaagcc aacccgaacg ccgggatcat tcagtcgtcg ccgaaagcgt
     6541 ccggtatgga tacgctgtat gcgcgctgtc agcagttcgc gacccgcgtg tatgggccac
     6601 tgtttacagc cggtttgcac ttctggcaac ttggcgagtc gcactactgg ggacataacg
     6661 cgattatccg cgtgaaaccg tttatcgagc actgcgcact ggctccgctg ccgggcgaag
     6721 gttcctttgc cggttcaatc ctgtcacatg actttgtaga agcggcgttg atgcgccgtg
     6781 caggttgggg ggtctggatt gcttacgatc tcccgggttc ttatgaagaa ttaccgccta
     6841 acttgcttga tgagctaaaa cgtgaccgcc gctggtgcca cggtaacctg atgaacttcc
     6901 gtctgttcct ggtgaagggt atgcacccgg ttcaccgtgc ggtgttcctg acgggcgtga
     6961 tgtcttatct ctccgctccg ctgtggttta tgttcctcgc gctctctact gcattgcagg
     7021 tagtacatgc gttgaccgaa ccgcaatact tcctgcaacc acggcagttg ttcccggtgt
     7081 ggccgcagtg gcgtcctgag ctggcgattg cactttttgc ttcgactatg gtgctgttgt
     7141 tcctgccgaa gttattgagc attttgctta tctggtgcaa aggaacgaaa gaatacggcg
     7201 gcttctggcg cgttacatta tcgttgctac tggaagtgct tttttccgtg ctgctggctc
     7261 cggtacgcat gctgttccat acggtcttcg ttgtcagcgc gttccttggc tgggaagtgg
     7321 tgtggaattc accgcagcgt gatgatgact ccacttcctg gggtgaagcg ttcaaacgcc
     7381 acggctcaca gctgctgtta gggttagtgt gggctgttgg gatggcgtgg ctggatctgc
     7441 gtttcctgtt ctggctggca ccgattgtct tctcgttgat cctgtcaccg tttgtttcgg
     7501 tgatttccag ccgtgccacc gttggtctgc gaaccaaaca ctggaaactg ttcctgatcc
     7561 cggaagagta ttcaccgccg caggtgctgg ttgataccga tcgcttcctt gagatgaatc
     7621 gtcaacgctc ccttgatgat ggttttatgc acgcggtgtt taacccgtca tttaacgctc
     7681 tggcaaccgc aatggcgac
//
LOCUS       DAJTYD010000176         7619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTYD010000176 DAJTYD010000000
VERSION     DAJTYD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.176"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..414
                     /gene="rutR"
                     /locus_tag="OO585_003414"
     CDS             <1..414
                     /gene="rutR"
                     /locus_tag="OO585_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6630888.1"
                     /translation="IWLAPLKAFREDFAPLAAIKEYIRLKLEVSRDYPQASRLFCMEM
                     LAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQHLIFMIWASTQHYADFA
                     PQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(454..4416)
                     /gene="putA"
                     /locus_tag="OO585_003415"
     CDS             complement(454..4416)
                     /gene="putA"
                     /locus_tag="OO585_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6630889.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            4471..4680
                     /locus_tag="OO585_003416"
     CDS             4471..4680
                     /locus_tag="OO585_003416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630890.1"
                     /translation="MMLQFCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            4839..6347
                     /gene="putP"
                     /locus_tag="OO585_003417"
     CDS             4839..6347
                     /gene="putP"
                     /locus_tag="OO585_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6630891.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(6722..6805)
                     /gene="yohP"
                     /locus_tag="OO585_003418"
     CDS             complement(6722..6805)
                     /gene="yohP"
                     /locus_tag="OO585_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6630892.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            7029..>7619
                     /locus_tag="OO585_003419"
     CDS             7029..>7619
                     /locus_tag="OO585_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCR6630893.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQT"
BASE COUNT         1695 a         2029 c         2084 g         1811 t
ORIGIN      
        1 atctggctgg caccgttaaa agcgtttcgt gaagatttcg ccccgctggc ggcgatcaaa
       61 gagtacatcc gtctgaagct ggaagtctca cgcgattatc cgcaggcttc gcgcctgttc
      121 tgtatggaga tgctggcagg cgcgccgctg ttaatggatg aactgacggg cgatttgaag
      181 gcattaattg atgagaaatc ggcgctgatt gccggttggg tcaaaagcgg caaactcgcg
      241 ccgattgatc cgcagcattt gatttttatg atttgggctt ccactcaaca ttacgccgat
      301 ttcgcccctc aggtggaggc ggtgacaggc gcaacgttgc gcgatgaggt atttttcaat
      361 caaacggttg aaaacgtgca gcggattatt attgagggga ttcgaccacg ttaaagatgc
      421 cggaggaggg tgtaacatcc tccggctacc tgtttaaccg atcgtcatta aactggcgtt
      481 accgccagcg gcagcggtat tcacactcag cgaacgttcg atatacagcc gttccagaag
      541 gatattgctt tcgccacggg caaaaccctg caccgaaaca attgctccat cccgcgcagc
      601 aacggcttca cacaatgcgc gaagctgatc cgaatcaccg tggaagatca ccgcatcaaa
      661 gggttgagcg gttatatttt ccgctttcgc cagttgaata cgttcgctga ctgccgatgg
      721 caatgccttt actagctgac gatgcagcgc gtcatccggc cacagtacct ggctgcccac
      781 cgccagcacg gcggcgagct gagtcagcgc atcctgctca tcatcggcaa tacacaacac
      841 gcgctcacgc ggcagcagcg tccaggtgtt gcgttcaccc gtcggccccg gcagcaatcg
      901 ttgtattcct gcctgcgcca gctcgccata ttgcgtacat aacgcctgca attctggacg
      961 atttgctgcc cattcccgca gtgcatttag cggctgagtc aatgcggctt tcaactgtgc
     1021 atcgaccgga tactctgcat cctgacgcgc gagcgtcact gccagcgcac tttccgggcg
     1081 attcgccagc agacggtaga gatagagcgg accgcctgct ttcggcccgg taccggacaa
     1141 cccttcgccg ccgaacggct gcacaccaac cactgcgccc accatattac ggttaacata
     1201 caggttacca acatgggccg agccagtgac ctgggcgatg gtttcatcaa tacgcgtatg
     1261 gacaccaagc gtcagaccat agccggaagc gttaatctgc tcgatcagct ctggtagctg
     1321 gttacggttg taacgcacca catgcagcac cggaccaaag acctctttgt gcaattcggc
     1381 aaagtcatcc agttcgatca gcgtcggggc gacaaaggtg ccgctttgcc attcacgggc
     1441 atcttcgctg ttttcccgca ccgcctggaa caccggacgg cctttgctac gcatggtctg
     1501 aatatggcgc tcaatattgg ctttcgcttc gctatcaatc accggaccga tatcggtggt
     1561 caggcgaccc ggattaccca tccggcattc ggccattgca ccgcgcagca ttttcaacgt
     1621 gtggtcggca atctcatctt gcaggcacag cacgcgcagt gccgaacaac gctgacccgc
     1681 actgtcgaac gccgaggcca gcacatccac gacgacttgt tcggtcagtg ctgaagaatc
     1741 gacaatcatc gcgttcatgc cgccggtttc agcgatgagc ggaatagggc gaccctgagc
     1801 gtccaggcgg ctggcgatat tgcactgcag taacgtagcg acttcggttg aaccggtaaa
     1861 catcaccccg cgcacgcgat catcacccgt cagttgcgcg cccacggttt ccccctgacc
     1921 tggcagcaat tgcaccacgc cagggggtac acccgcttcc agcaaaatgg cgatcccttg
     1981 cgcggcaatc agcggcgttt gttctgccgg ttttgccagc acgctgttac ctgccgccag
     2041 tgcggcagcg atctgcccgg tgaaaatagc cagcgggaag ttccacggac tgatacacac
     2101 cacaggccct aatggacggt gggtttcgtt agcgaaatca tcccgcacct gtccggcgta
     2161 gtagtggaga aaatcgaccg cttcgcgcac ttcggcaata gcgttactga aggtttttcc
     2221 ggcttcacgc accagaatac caatcagttg ctgcatctgg ctttccatta gcacggcagc
     2281 gcgatgcaaa atcgccgcgc gttcagccgg aggcgtggca aaccagattg gcgcgttatt
     2341 aaccgcactt tccagcgcct gttctacttc acgcggcgtg gcttcacgca caaagcccac
     2401 aatatctttc ggttccgcag ggttaataac gggcgacatc tcacctgccg ctaccggttg
     2461 ttccagcatt ggcaaggcct gccatttttg cagtgcacta ttgagcaggg cagaggagag
     2521 cgaggccaga cggtgttcgt tagcgagatc cagccctgcc gagttgtcgc gcccgtgacc
     2581 gtaaagatcg cgcggcaggg gaattttcgg atgcggtaat ccagtttgcc cttcctgttg
     2641 cgccagtttt tctacagcag tgaccggatc ggcgaccagt tcatccagtg gcaaagaggt
     2701 gtcggcaata cggttaacaa acgaggtgtt agcaccgttt tccagcaggc gacgcaccag
     2761 atacgccagc agcgtttcat atgtgccaac cggggcataa atacgacacg gtcggttaag
     2821 tttaccgtcg gcaactttcc ctgtgacctg ctcgtacagt ggctcgccca taccatgcag
     2881 gcactggaac tcgtactgac ccggatagta gttctgacct gccagttgat aaatcgctgc
     2941 cagcgtatgg gcgttgtgcg tcgcgaactg cgggtagatt agattcggca ccgccagcag
     3001 ctttttcgca caggcgagat aagaaacgtc ggtatacacc ttgcgggtat aaaccggata
     3061 gccttcaagg ccgtccatct gcgcacgctt gatttcacta tcccagtacg cgcctttcac
     3121 caggcgaatc atcagacggc gacggctgcg ggtggcgaga tcaaccaggt aatcgatcac
     3181 caacgggcag cgtttttgat aagcctgaat aacaaaaccg atgccgttcc agcctgccag
     3241 ttccggctcg aaacagagat tttccagcag atcgagggag atctccagac gatcggcctc
     3301 ttcggcatcg atgttaatac caatatcata ctgacgcgcc agcagggtga gtgatttcag
     3361 acgcgggtaa agctcttcca ttacccggtc atactgggcg cggctgtagc gcggatgcag
     3421 cgccgacagc ttgattgaaa tgcccggacc ttcatagatg ccacgaccgt tagacgcttt
     3481 accaatggcg tgaatcgcct gctgatagga aaccatatac gcctgtgcgt ctgcggcagt
     3541 cagtgcagct tcgcccagca tatcgtaaga gtaacggaaa cctttctctt ccagcttgcg
     3601 ggcattggct aacgcttccg cgatggtttc acccgtgacg aactgctcgc ccatcaggcg
     3661 catcgccata tccacacctt tgcggatcag cggttcaccg cttttaccga taatgcggtt
     3721 cagcgagcgg gagaggctgg cttcgttatg ggtggaaacc agtttgccag taaacaacag
     3781 cccccaggta gcggcattaa caaacagtga cgggctacga ccaatgtgtg actgccagtt
     3841 acagttgctg attttgtcgc gaattaacgc gtcgcgggtg gctttgtcgg gaatacgcaa
     3901 caacgcttcc gccagacaca tcagcgccac gccttcctgc gatgacagcg aaaactcctg
     3961 caataaccct tggaccatac ctgcgcgacc actggcattt ttttgattac gcagtttatc
     4021 ggccagttga tatgccagtt tatgcgcctg ttcagcaact ggctgcggca ggcgggcttg
     4081 ttccagcagc atagaaaccg cttcggtttc cgggcgacga taggccgcgg tgatcgcggc
     4141 gcgggaaacc gactggggca aaatttgctc ggcaaagtcg aggaatggct ggtgtggttc
     4201 ctctgccgga gtcggtgctt catcgctctc attggcagca ccagaaagca gcgcaggtag
     4261 ctctggcaga gtatcgctgt tttccagttg ttcgagataa gaaaaaatcg cctgcttaat
     4321 taaccagtgt ggcgtgcgat cgatacgtgt cgcggcagac ttaatacgct cacgcgtcgc
     4381 gtcgtccagc ttaaccccca tggtggtggt tcccatgcca ttactcctgt tgttctgaaa
     4441 ggtgcaactt aacgttatcg tgaaatatcc atgatgttgc aattttgtgc aaccatgtta
     4501 aatgtgacct gcgtagcaag cttaaaaatg aatgaaatgt taataaaaga aattgatatg
     4561 acagggatta aaaaaataac tcagactttt tctctgcggc agttaacatt tttgaaaggt
     4621 gcaaccgcaa aaaatgtgag agagtgcaac ctgatgaaaa atagtgtcgc tgagcactaa
     4681 aatttaatgt aaatggtgtg ttaaatcgat tgtgaataac cagcgcttcc ggcaggatac
     4741 ggtcgccctg gtaaaaaata aactctgtta ccccgttccg gttgcagata taacggcaag
     4801 tttcgacatt gccgataata attttttgga gactttagat ggctattagc acaccgatgt
     4861 tggtgacatt ttgtgtctat atctttggca tgatattgat tgggtttatc gcctggcgat
     4921 caacgaaaaa ctttgacgac tatattctgg gcggtcgtag tcttgggcca tttgtgacgg
     4981 cattatcggc gggtgcgtcg gatatgagcg gctggctgtt aatggggttg cctggcgcgg
     5041 tttttctttc cgggatttcc gaaagctgga tcgccattgg cctgacatta ggcgcgtgga
     5101 tcaactggaa gctggtggcc gggcggttgc gtgtgcatac cgaatacaat aataacgcct
     5161 taacattgcc ggattatttc accgggcgtt ttgaagataa aagccgcatt ttgcgcatta
     5221 tctccgcgct ggttattttg ctgttcttca ccatttattg cgcttcgggc attgtggcag
     5281 gcgcgcgtct gtttgaaagt acctttggca tgagctacga aacggctctg tgggcgggag
     5341 ctgcggcgac gatcctttac acctttattg gcggtttcct cgcggtgagc tggactgaca
     5401 ctgtacaggc cagcctgatg atttttgccc tgatcctgac accggttatc gtcattatca
     5461 gtgtcggtgg ctttggtgac tcgctggaag tgatcaaaca aaagagcatc gaaaacgttg
     5521 atatgctcaa aggtctgaac tttgttgcca ttatctcact gatgggttgg gggctgggtt
     5581 acttcgggca gccgcacatt ctggcgcgtt ttatggcggc ggattctcac cacagcattg
     5641 tccatgcgcg tcgtatcagt atgacctgga tgatcctctg cctggcaggg gcggtggctg
     5701 tcggcttctt tgggattgct tactttaatg agcacccggc ggtagcaggc gcggtaaacc
     5761 agaacgccga gcgcgtgttt atcgaactgg cgcaaattct gtttaacccg tggattgccg
     5821 ggattctgct gtcggcaatt ctggcggcgg taatgtcaac cttaagttgc caactgctgg
     5881 tgtgctccag tgcgattacc gaagatttat acaaagcgtt tctgcgtaaa catgccagcc
     5941 agaaagagct ggtgtgggta gggcgtgtga tggtgctggt ggtggcgctg gtggcgattg
     6001 cgctggcggc aaacccggaa aaccgcgtgc tgggcttagt gagctacgcg tgggcaggct
     6061 ttggcgcggc gtttggtccg gtggtgctgt tctcggtgat gtggtcacgc atgacgcgta
     6121 acggtgcact ggcggggatg atcatcggtg cgctgacggt tatcgtctgg aagcagttcg
     6181 gctggctggg actgtacgaa attattccgg gcttcatctt cggcagtatc gggattgtag
     6241 tgtttagttt gctgggtaaa gcgccgtcag cggcgatgca aaaacgcttt gccgaggccg
     6301 atgcgcacta tcattcggct ccgccgtcac ggttgcagga aagctaaggg acttagcctg
     6361 cggcggtttt gtttggcttc agcagcgggt tgcgctccct taatgtgcct cgccatataa
     6421 attgaatggt gcagggagcg cgcagggggc ggccaatcgc cgccgccccc tgtgcacccc
     6481 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggctac
     6541 cgggtcgggc gcgaggtaac gtccttgtaa gacgcgccct cagcccacat ccatgtgggc
     6601 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccg gagctgtttg
     6661 caaatcaagt acacaaaata tctagttcct gacctgacaa cttatatgcc atatctgatt
     6721 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
     6781 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgtt gaataagtat
     6841 aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc ggaagatatc
     6901 attcacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
     6961 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc ttacctgatt
     7021 attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg ctgttattga
     7081 tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag caaacgcccg
     7141 ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca gacagccaat
     7201 ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca ctacagaacg
     7261 caccggatat gcaggtggct gaacagcgca ttcagctagc tgaagcccag gcgaaggcag
     7321 ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg caaaaaatgt
     7381 cggcagaagg cttaatgggg ccgtttgctc tgaacgatcc ggccgcaggt acgaccggcc
     7441 cgtggtacac caacggtact tttggcttaa cggcgggctg gcatctcgat atctggggaa
     7501 agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg
     7561 agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag tggcaaacc
//
LOCUS       DAJTYD010000177         7557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTYD010000177 DAJTYD010000000
VERSION     DAJTYD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.177"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            555..3968
                     /locus_tag="OO585_003420"
     CDS             555..3968
                     /locus_tag="OO585_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6630894.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4032..5666
                     /locus_tag="OO585_003421"
     CDS             4032..5666
                     /locus_tag="OO585_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6630895.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5675..6745
                     /locus_tag="OO585_003422"
     CDS             5675..6745
                     /locus_tag="OO585_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6630896.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6870..7085)
                     /locus_tag="OO585_003423"
     CDS             complement(6870..7085)
                     /locus_tag="OO585_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630897.1"
                     /translation="MGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDE
                     AMNTQVLSSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2203 a         1721 c         1779 g         1854 t
ORIGIN      
        1 cccaaatatc atggtattga tacgggagat tatgccgtac aggtacgaca cgttatgtcg
       61 tgccaaaggg gagtggatag aaaaaatggc gaagatgcga aaagttgatt aatgcattga
      121 aaaaaatgaa ttaataatga acttgggtaa aagcgccatc aaaatacgac acgacctgtc
      181 gtattttgtg ttagccggga aaagatatct gctacttact aaaatgattt agtgctgaaa
      241 atatttataa gccaataaat agacaggtat tacggagtaa cgcccaggct attttaacga
      301 gcctgctgtt ttattggctc aaaaaacata tcccatagat gatcagccat acccgcgtta
      361 gtgagcaagg tatcccttga gtagccaggg ctgaagacgc ccattgatgg gcgaaaacca
      421 aggttctggc cgttaaagcg gctatgtcgt taaacacatc accaccatat gccatccggc
      481 tcacttcgtc ggccaaatta gtgtgatatt ctttccagaa tttctgttgt atccacaaca
      541 taaggcgatg attgatggag cagtctctca attttgaaat gttgcgcagc cagtggccgg
      601 aactggcaga actcgcatgt atggcagagc gctatgttca ctccgatccg gaaagctgcc
      661 tggttaagct gcgcaactac accgaattga tggtgcgctg gttgtatcgt caggagcggt
      721 tgccggaagg tattaaggct aatctttacg atttaatgaa cgctgatgtc tttaccagca
      781 tgatgccgga agccatcatc atgaaaatgg atgcgctgcg tatccatggc aaccgtgccg
      841 cgcacggcgg acgtatcaaa gctaaagata cttactggct gctcaaagaa gcgtatttgt
      901 tgggaatttg gctgtatgtt cgctacgccc acggtaatgt tgatgactgc cccaaattta
      961 cactccctcc attaacacaa tcttcaggtc gtgcagatga aaaacgtctg gaagatgcaa
     1021 tcaaggctca ggatgaaagc cgtgagcgcg aactggcgct acaacgcgca ctacagcaag
     1081 aacaggaaaa ggccgaacac ctcactcaac gcctgaatga agccagagcg cgtaaccagc
     1141 atgttgccga tattctctct attgatgaag cggaaacccg ccgtcgcctg attgactctc
     1201 gcttacttgc agccgactgg aatgtagggg aagaacttaa aaacacggat caagtcacac
     1261 aagaacatcc agtcaaagag caacccacca cctctggtga cggttatgca gactatgtct
     1321 tgtgggatga ggcacacaaa ccgttggcgg tggtggaagc aaaaaaaacc agcgtcaatg
     1381 ccgagcaagg acgaattcag gcccggctgt atgcagactg gctggaaaaa gaatacggtc
     1441 agcgtccggt catcttctac accaacggct atgatatctg gctgtgggat gaccataaaa
     1501 ctcatggtta tcccccgcgt cgggtgtttg gcttctacag caaggaaagc ctgcaatatc
     1561 ttattcagca gcgtgaaacc cgtctgccgc tgaacagagt gccacacgta aaagataacg
     1621 aaggtaaggc cgttgccgga cgtttgtacc agctcgaaac cattgcccgc gtcagtgaac
     1681 ggtttaccaa caagtaccga caatcgttaa ttgttcaggc tacgggtaca ggcaaaaccc
     1741 gcgtggcaat tgcactaagc aaactgatga ttgatgcccg ctgggtaaaa cgcgtcctgt
     1801 ttctttgcga ccgtaaagaa ctgcgtaaac aggcggcaaa tgcctttaat caattcacca
     1861 atgagccgct gtatgtggtc gggaaatcga aaaaagcaga caggcagaat gccagaatct
     1921 acatcgctac ctatcccggt atgatgaaaa tcatggacca tttcgatgtc ggttatttcg
     1981 atttgatcat cgccgatgaa tcccaccgct ctatctacaa cgtctacggc gatctgttta
     2041 agtattttga tgccctccag attggcctga cggccacgcc aattgacatg gtgagtaaaa
     2101 ccactttcgg cctgtttggt tgtgaaggac gtattcctac cgccaactac agcctggaag
     2161 atgccatcgc cgataacaat ctggttccct atgaggttgt cacacacaca acagaattcc
     2221 tgcgcgaagg tatcaaacgg gagaaattaa gcgacgcaca aatccgcgag ctggaagaac
     2281 aaggcataga tcccaatacg ctggagtttg atggcaaggc gctggatgag gcgatctaca
     2341 acaaagacac caaccgctat atcctgcgta acctgatgga aaatggcctg aaagatcggg
     2401 acggccagtt gcctggtaaa accatcattt tcgcccgtaa ccacaaacac gcgctgctgt
     2461 tgaatgaatt gttcgacgat atgtacccac agtttgccgg gcgcttctgc caggtcatcg
     2521 acaactacga tccccgcgcc gagcaactga ttgatgattt taaagggctg gatgaaagca
     2581 ccaacaaaga gctgaccatc gctatctccg ttgacatgct cgataccggt attgatgtcc
     2641 cggaaattgt gaatctggtc tttgccaaac cggtcaaatc aaaagtgaag ttctggcaga
     2701 tgattggccg tggtacgcgc ctctgtccgg ggttgtacgg ttacgacgat aacggcaaac
     2761 cgctggataa acagaaattc cgcattttcg atcactgggg caattttgag tatcacgagc
     2821 tgcataccga agaggcggaa gtcacagcga caaaatcgct tgcgcaaaaa cgcttcgaag
     2881 cgtgggttat gctgggggcc gctgcgcagc gtaaattcga caaacaggcg gtggatttag
     2941 ttgctcacca gcttcgcgag caaatcaacg cactggatga aaagtcgatt gccgtacagg
     3001 aaaaatggca gcagaaagcg cagtacagtg atgaaaaggt gctgcgccag ctttctccga
     3061 aaacacagca ggatctgcta tctgttctgg ccccgttgat gcagtggctg gacgtgcgcg
     3121 ggcaaagcga tgccatacgc tttgatatgg atattctggc ggcgcaaact gcccgttata
     3181 ccaacccgga agagctggat gttctctggc cgatcatcgt cgaaaaagtg gagcgcctgc
     3241 cgccgcattt ggcgcaggtg cagcaacagg gacaacgaat caatcagctt cgtaatttaa
     3301 gctggtggaa gcaggccagc ctggaagagc tggaggatat tcgcattcat ttgcgcggta
     3361 tcatgcacct gatggaaaaa gacgcgacgc ctaaatttgg ttcgatacag gtggatatta
     3421 ccgaagatgc gaacctgatc cagacggaga cccgcaaaac taatatccgc tcgattgatt
     3481 tcaaactcta tcgccagcag gttcaggggg cgctggagcc gctgttccag caaaacccgg
     3541 ttctgaagaa aatccgcaac ggcgagccag tcacgcaaaa cgagctggat gagctggcga
     3601 agctggtgct gatccagaac cccaacgttg atattcgtgc gttgaaagag ttttatccgc
     3661 aggcaaccgc cagcctggat aaacttttgc gtaccatcat cgggatggac agcgacgcgg
     3721 tggaagtgcg ttttgcccag ttcgccgctg ataacagcct gaccagccaa caactgcgct
     3781 tcctgtcatt gctgaaaaac cacattcgcg attacggcac cattgaaatg cggcagctct
     3841 ttgaacagcc ttttacgcat atccacaacg aaggcgttac cggtgtgttc cctgatatag
     3901 cgcaaatcgc ccgcctgcaa aagatagtcg aagagctggg tgttgtgacc gacgcagcga
     3961 cggtataata cacgcatgac aagccccggc agcggggctt ttgtatttaa aacaggatat
     4021 aaacgctaac aatgattact ggcgacttaa aaagtaaaat cgacggacta tgggaagatt
     4081 tctgggtggg tggtatcacc aacccgctga ccgtaatcga acagatcacc tatctgatgt
     4141 attcccggat gctggatacc caggaacaac gcgacgaaaa gcgtaaacaa atcgcgggta
     4201 ttgattttaa accgcgtttt gcgccagaac agcaggagtt ccgtttcagt cactatagca
     4261 accttggctc ggatgagatg atggaagtgg tgcgcgatgg cgtattccag catttccgtc
     4321 agctcggcca ggccgatgct tcgaaggtga cgctgctggg caactttatg aaagatgctc
     4381 gtctggagat tgttaagccg tcattgctga ccaaagcggt tgaggtaatc aaaaacctgc
     4441 cattggatcg cggcgacacc aaaggcgacc tttacgaata cctgttaagc aagctgacaa
     4501 ctgccggaat caacggacag ttccgcacac cgcgccacat tatccgcacg atggttgaaa
     4561 tgatggagcc gaacccggcc cgcggcgaga cgatttgcga tcccgcctgt ggcaccggtg
     4621 gttttctggc aaccagctat gaatatctgc tggagaagta cagctcgctg gaatccattc
     4681 atactgagat tggcaccaac gaacgtggcg agctggaaga gcaaaaaatc tttaccggcg
     4741 atctgctgac accgtggcgt aaccatgtgg ataacaacat gttccacggt tacgactttg
     4801 acaccacaat gctacgtatc gccgccatga acctgattat gcacggcgtg gatgcgcctg
     4861 atatccacta tcaggacaca atgagccaga gtttcagcac aaacttcccg caggccagta
     4921 aaaacgcctt caacctgatt ctggcgaacc cgccgtttac cggttcactg gacgaggaag
     4981 atatcgactc cacgctgtcg gcaatggtga aaaccaaaaa aaccgaacta ctgttcctgg
     5041 cgcgtattct gcaaatgctg aaagtgggcg ggcgcagtgc cactatcgtg ccgcagggcg
     5101 tgctgtttgg ctctagcaag gcgcaccagt cacttcgcaa aacgctggtg gaagataacc
     5161 aactggaaac ggtgatcaat ctgccttctg gtgtatttaa accttacgct ggcgtggcga
     5221 cggcgatcct gatctttacc aaaggcggtc aaacggatga ggtctggttc tacgatctac
     5281 aaaatgacgg ctacagcctg gatgataagc gcaacccgat aaaagacaac gatctgccgc
     5341 atctgctggc aagctggaag cattaccgta ctttacgcgg gctaccggtt gataacttta
     5401 tgggtaagaa gttagcctcg ttgcttaaac agcagtaccc ggaagggatt aatgctggcg
     5461 ttgattttaa agatcgcacg caggcggcgt ttgttgtacc gaaagcggat attgctgcgc
     5521 agaaatacga tctatccatc aaccgttata aagaagtcgt gtatcaggcg gaggaatatg
     5581 aagatccgaa ggtgatattg aagcggttaa aggatctgga aaaagagatt ctggcggatt
     5641 tggatgagct ggaggggatg ctgtgagttc ggcgatggtg aagttagggg atgttattaa
     5701 tgttcactac ggaaaagcat taaaagctga tcagcgtgta tcgaatggaa gtgtccatgt
     5761 gtttggtagt tcaggaattg tcggcaacca cgataaaacg ctttgcagct atcccacgat
     5821 aattattggc cgcaaaggca gcgttggtgc tattacctgg gctccaagcg gaggctggat
     5881 cattgatact gcttattacg ttgagatcaa agataataat aagttagatc tccgatactt
     5941 gttttatatt ctgagcggaa ttgatctcac taagaaaacg attaccacaa gtattcctgg
     6001 attaaataga gacgatctct atgatacatt tattaaatta ccaccttttg aagagcagaa
     6061 acgaattgtg gatcttctcg ataaagctga gggtatacgc caaaaacgtg agcaatccat
     6121 caaactggct gatgattttt tgcgtgctac gtttgctaca atgtatggga atcctattac
     6181 aaaccccaaa aaatggcctg tccatcttat gggagaaatt attgaattta aaggtggaaa
     6241 tcaaccacct aaaagtgatt ttatttttga acctaaacag ggttatatta gattagtaca
     6301 aattagagat tttaaatccg ataaatatgc gacgtacatt ccgcaagaaa aagcaaaacg
     6361 tattttcgaa gttgatgatg ttatgattgc tcgttatggg ccacctgtgt ttcaaatact
     6421 tagaggttta tccggcagct acaatgtagc tttaatgaaa gcttcaccca aagaaaatat
     6481 taggaagggg tttatattct atcttctaca acttccggaa tatcatgatg ttgttgtaaa
     6541 aaactctgaa agaacagcag gacaaaccgg tgtaaatctt gaactattaa acaaattcaa
     6601 tgttcccctc ccacctatat actatcaaga tgagatatta gatagattag ctagaattga
     6661 aaaatttaaa gaaaaaatag aaatatcatt aaatcattta gaaatacaat ttttatcact
     6721 acagaagaga ttaataggtt tttaaatcct tagtatcgaa ataaacatta atttattata
     6781 ttaaattaag atattttagg aaattaaggc gtcactctta ggtaacgcct ttttttacta
     6841 tctcgccccc taagaaggtt atatcttact taatacctgt gactattttt gatataatca
     6901 taaatacaca ctaatttgca aaaattactt gacaatacct gagtattcat agcttcatct
     6961 atatgttttt tcaatatttt gagaagctct cccatatcgc gcttgattaa tccaggctcc
     7021 atataaggca tcattcttaa aaagaaactg aaatcataag caagatataa ctcattgacc
     7081 cccaccttga cctcaggaac ggtttcagag cgtcgtttat cccatccttg agactgaaaa
     7141 ctaataggag tgatatttaa tcgtgataaa acgccacctg agagttctgt ttcattaatt
     7201 atactcccat caggattaat taatttagcg tgatttaaga agtgaaaaat aggaatgcgc
     7261 gcattgcctt tgcatatatt tggcaaaaca tcattaacaa cacatcccca atcataatat
     7321 ccattttcaa atacaacctc tcggatgaga tctacacgct ccagtccaac caatccttta
     7381 gttgccatcg attccgattt ttttaccatc gaaatagcac catatcccac aacaaactca
     7441 gttgtactga ccccttcttc aattttctct gaatccctca catataattt tcctttcgga
     7501 tcatcagtta agaccaactc ataaagttca tcttttatca ttcggaataa atgtgct
//
LOCUS       DAJTYD010000178         7474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTYD010000178 DAJTYD010000000
VERSION     DAJTYD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.178"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..340
                     /locus_tag="OO585_003424"
     CDS             <1..340
                     /locus_tag="OO585_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6630898.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            715..1149
                     /locus_tag="OO585_003425"
     CDS             715..1149
                     /locus_tag="OO585_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6630899.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1160..1801
                     /locus_tag="OO585_003426"
     CDS             1160..1801
                     /locus_tag="OO585_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630900.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1798..2148
                     /locus_tag="OO585_003427"
     CDS             1798..2148
                     /locus_tag="OO585_003427"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630901.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRIETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2842..3453
                     /locus_tag="OO585_003428"
     CDS             2842..3453
                     /locus_tag="OO585_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630902.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3991..4533
                     /locus_tag="OO585_003429"
     CDS             3991..4533
                     /locus_tag="OO585_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR6630903.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4526..4885)
                     /locus_tag="OO585_003430"
     CDS             complement(4526..4885)
                     /locus_tag="OO585_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630904.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5319..6143
                     /gene="dinD"
                     /locus_tag="OO585_003431"
     CDS             5319..6143
                     /gene="dinD"
                     /locus_tag="OO585_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6630905.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6435..7052
                     /locus_tag="OO585_003432"
     CDS             6435..7052
                     /locus_tag="OO585_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6630906.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7049..7474)
                     /gene="ligB"
                     /locus_tag="OO585_003433"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7049..7474)
                     /gene="ligB"
                     /locus_tag="OO585_003433"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1999 a         1835 c         1909 g         1731 t
ORIGIN      
        1 gcagacggag gcgtttttga atgcctatgg gctgagtcga ttcgcgccgc tgggttatga
       61 cccgcgcgat ttacccatcc gtgatttagc cgggtacagg aagaagggga accacgacgg
      121 cgacccgata attttctaca ccttcccggc agcgtttgag caggagattg caaagggctt
      181 caataccaaa cagtttgccg aagttctaaa gaatgcagga atgcttacac caccaacgag
      241 cgggcgaggc tatcaggggc gagtaaggga ggatgggcga cagattcgcg tctatgtact
      301 caatttcatg gcagaggaaa gcagccagcc agaagaataa ttatttcttc acatgcgtag
      361 ttttagtgtt gtctcggttg tctttgttgt ctcaatttgt aattaagatt gatttataag
      421 gattttattt taatttctga gacaacaccg agacaacatt tagccatttt gagacaacag
      481 ggcgaaagtg tgtttttagt ctggcaggca gtgagacaac atttatgccg ggtgagacaa
      541 cacgaaattg caaatgttgt ctcagcaaaa cccagacgtg gcgcggcctg tagagccgtg
      601 agacaacaaa gacaacagag acaacactat ttttgcttac tatgaaaaaa aatcggcagg
      661 tgtcccacat taagaaatcg tcattagcaa aactaataat ccagtgaggg gaaaatgaca
      721 gcacaaattt cagcatacgg gcggctggtg gcagatcccc agacccgcac cacaacgaac
      781 ggtaacaaca tggctatggc gcggctggcg gtgtctctcc cctgtaatgc ggcagaggcg
      841 ggagagtcaa ccttctggct gggcgttatt gcttttggga agcaggccga tgcgctggca
      901 aagcaccaga aaggcgacct tgtgagcgtg gcaggcaata tgcaactcaa ccagtggaca
      961 gggcaggacg gcggtacaca gcaggggtat caggtagtag ccgacagtgt gatcagcgcc
     1021 agaacggtac gccccggcgg gaaggcagga cagcggggac aggcaacgga cgcgctgaga
     1081 cgtgcgcagc agccatcccg tgatgaatac gaccaacgcc caccattcga cgatgaaacg
     1141 ccattctgag agggaagata tgagtacgca acccatcatt attgagttcg gcagctatcg
     1201 ctggcccaga catcttgcaa tacatgaagc agatcatgcg atagccgcat ggtttttcgg
     1261 catacaggag gtagaaattg cgttatcggg taataaccga gtggcaaaaa cctaccttag
     1321 cggcgatgtt gatgaatgtg gagctgtcgc ggtatggcac gccaactatc cacggaatgt
     1381 ggctgaggtt aagtcttaca ccctctcgaa tgatgacacc aggttacgtc tgatagacca
     1441 ggtatgccgg gctatggtta tctctctggc tggtatcgtc gcgcaatcac gctatacggg
     1501 ggatgatatt aacgcactga tggaaacgtc cgggaaagca gacatgaaaa atgttcgtga
     1561 tttatcagag gtataccggg tggctggtgg ggaggatgct gatatacaga gaatatccct
     1621 ttcccgtgct gaggcgctga ttgccctgaa atggtgggat gttatcgcgc tggcgaaaat
     1681 actggagaac cgtaaccata tgcctgccag caacttccag cgcattatgg gtgatattga
     1741 caagcctgta ccaaagccta tgacgctggc tgaactggat gaattggtgg gtgaagaatg
     1801 aagccataca ttctggcagc cggcctgatc tcctcggcac cggcactatc tgctatccat
     1861 tgtgggggct ttaccataca gccagaccgt tatcggacaa cgattaacgg cgatgtggtt
     1921 catattgttg atgcgcaatt ttacggcgca ccgcgagact ttagccaggc cgccataaag
     1981 ttattgccgg atgccattac cactaccgat aggcaatacc gcatagagac gcagggaggc
     2041 aatgccttgc ttgagttgat gaccagagaa aacccgccac gtgtgcttaa tcgtgagcgc
     2101 tgcgatgccg ggttaacaag tttcggttgg tacacttctg cgccgtgatg ttcgttccga
     2161 gcgcagccta aaatattgga ctgttcttga cggatttaaa actctaatgg agccaagtcc
     2221 ttgccagtag cgggctagga gcctaattgt gattcaggtc gtcaaaaata ttcaggtttg
     2281 cggcattggt tcgcagtacg cagtccggta cgcggcaaaa ctgcgcattt aacgtgcgta
     2341 ccacctgacg aattctaacg ccggcattgc aaacagatgc gtgcgcaaaa tggctaagac
     2401 gtctgatatt gctggctttg gctcaatcag tttggaggtg gcggtatcag tacgatgcta
     2461 atttttgcta aggttttagt acgcaactca ggtacgcact ctcaccagat acgaaaccga
     2521 ttcgcagttg cgtaccagcc tgtaaggcgc attatcgctg ggtttctaca gtcgaatgca
     2581 gtacgcaaag tggaaaatcc tccgaatacg aaacccgagc attgcgcagt aaattgcgaa
     2641 ttgcgcagtt aacaaaatat ctccgcaaaa cgaaacggat atcaatgcgc aaaccatcct
     2701 caacccccgc gcggttactg gctttattga tgtgaaattg caaaattggt gcgcaattta
     2761 cagtaaaata cgcatattat ttcgtattca atttgcgcac cgttcgcagt taatgcgcag
     2821 taattcgcag gaggccagcc agtgagagac agaacgcgca gctataccac cgacctgccc
     2881 cgtatagggt tgccattcct tgcgaacatg cgtagcaggc tggcggatgc tgaaccgggt
     2941 acgcagcttt atacccagac cgaaagcgga acgttgtatg ccttccggca agccgacagc
     3001 tacgccatga caatcaacgg cgtgacgcgg gctattcgca ccacaaccac gaaggccggg
     3061 tatggcgtcc gggaatggta cgcttgcccc cactgtatga agcgggctgc aaagctgtat
     3121 atcggcaaga aagacattgg ctgccgggcg tgctggaaac ttcactataa aagccagagt
     3181 gccgacatac tggacaggat gcgcatgaag atacgccagc agcgatgcgc gatatggggc
     3241 agcaatgacc ttgccaataa tctgtttaat gacattcgca tgttcccaaa accaaagggg
     3301 atgcgctggg ctacgtttga caggaagcgg gcagagttat cagttatgga gatggcttac
     3361 tggcaggcat tcagcccggt agtggacaga atcacgggcg ttattgagcg taaagcagga
     3421 aaagcagcca gaggcattgg cctgactcta taaaagcaaa acgcccgcac ggggcgtcag
     3481 agaacgggat agacttctgc gacaatttgc attcgccgcc aatatagcaa gccgtccaca
     3541 tcaagcaata aactatcacc gggaggggga ggccaaatct ctgcaatcct gagccttaag
     3601 gactgcccgc cccatcgttt tttcataccc gcgaaaaatg aaatttaatc cgggcgtgtt
     3661 tcatccttcc agagggtatg acgtatgaca ccacgcgaaa tagccttatt gaccatcgcc
     3721 aaacttgagc atggaggcca ccagcttaca caggcagatc aacgggagat agaacgctca
     3781 gttaatgccg atatagcccg gcgcgacagg ttccgcgaaa tgatgcgagc acctgcctac
     3841 cagtggaaga agccagcgcc gcgcaggtag atacgggatt attccgcatg taacttatgc
     3901 gtattaagca aatttccttt attcaccttt acagaattgt gagtacgtca gtatcgttat
     3961 ccttccgatt cgcatttatt caggatttgc atgtaccagc taaagataac catccgagac
     4021 agcaaaccac ctatatggcg gcgtgtgctg gttccagagc aaatcccctt tagtgaactt
     4081 cacgcggtga tccagttggc ttttggctgg aatgacgaac acctgtatat gttcgagaaa
     4141 gggcgtaaag gtgatcccgg tagcgagtat cgcgtatggg gtgaggatga aagcatgggt
     4201 aatgcggcaa tcacaccact atgggcagcg ctccagaatg atggtgacaa gctggtttac
     4261 acgtatgact ttggcgactg gtgggattgc gtcattgtgc tggagaagca aacacacgat
     4321 acgagcaatc agcccattag ctgcctgcgt gggaaaggca ccaccccggt ggaaaactca
     4381 ggcggcttgc atggttacaa tgaattgcta ttacaggcca gagagtctga taatcctgag
     4441 caggccgaga tccacaattt cctgatgctg gatattgagc gccgggttta cgacctgagc
     4501 agcatcaacg acagattgca gactcttaac tgacccgcat atattcgcct gatattgcgt
     4561 tatcagcgcc gccagtatcg cgcataacag cgcactgacc gggcaaaccg tagcgcacga
     4621 ccgttgcgtt agtctcaccg ccgcgccgta tctggcgcga ttcatacact gtcacccgct
     4681 caagcagtcc accagcaacc atgctttcca gtgtgcgccg ggttgattcg agctggtgac
     4741 gcttatcgaa cgacaccatg ccatgaagca ggtaggccac acccgacaca tcgaacggcg
     4801 gcgcaccaat ctcaccagtc acccattcga ggttatccgg ttcaaagtag ctaagtatct
     4861 cttttttgcg gctggtcatt ctcatagctg gcaggttcct tattgtggga tagcactatc
     4921 atacaataag gcgcaaaaga taagaggcca gaacagcaag catgagagca taaaacgagc
     4981 agccaaagac agcgcctcaa aataccgtgt acacaagggt gtacctactg agaaaacgca
     5041 ttacaagaaa cacagcaacc acgggcgttt aaagccacta ctcaataaac agattcaact
     5101 cgtaacgcca attcttactt ttcagccttc acaaatgccg cccactcaaa cagagcggca
     5161 tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg ctccagattt tgcgatgttt
     5221 ttttgcccag cacactgaga acgtgagata ctcacaactg tatataaata cagttacaga
     5281 tttactttct ttgcaatcga tatcacatgg agtgggcaat gaacgaacat catcaacctt
     5341 ttgaagaaat acggcattat ggcactgaag ggcaagaatt ttggtcagct agagaattgg
     5401 caccgttact ggattatcgg gactggcgca attttcaaaa ggtactggcc cgcgcgactc
     5461 aagcgtgcga agccagcaat caagctgcat ctgaccattt cgttgaaacc accaaaatgg
     5521 ttgtattagg ttcaggggct caacgagaac ttgaagatgt tcatctctcc cgctatgcct
     5581 gttacctggt agtacaaaac ggcgaccctg cgaaaccggt tattgcggca gggcaaactt
     5641 attttgctat ccagacccga cggcaggagc tggctgacga tgaagctttc aggcaacttc
     5701 gtgaagacga aaagcgtctt tttctgagaa atgaattgaa agaacataac aaacaattgg
     5761 ttgaggcagc gcagcaagct ggggtagcga cagctatcga tttcgccata tttcagaatc
     5821 atggttaccg ggggctgtat ggtggattag atcagaaagc tattcatcag cggaaggggc
     5881 tgaaaaagaa tcagaatatc ctggatcata tgggttcaac agaactggcg gctaatctct
     5941 ttcgagctac ccaaacagaa gaaaaactca agcgggatgg cgtgaattca aaacagcaag
     6001 caaacaccac acactttgac gtgggtcgca aggtgaggca aaccattcag gaacttggcg
     6061 gaaccatgcc tgaggagttg ccgaccccgc aggtcagcat caagcagttg gaaaacagtg
     6121 taaaaattac agagaagaaa tagcagaaac taaaaactct ttttcttaat tgagataccc
     6181 gatgcgtaag ccaaagtcca ggtgcagtga acacaatggc taaacattgc acctttcttt
     6241 tccacccctc aattttaacc tattttttct tatgcatttt ctcagacaag aactcagaat
     6301 aatgcatcta cgctacagaa aatagcgatt tcacataact acaagttatc aatttcctct
     6361 taccttaaaa aatctcaatc gtgacaatgc gcacaaatcg ctaccctgcc agacagattt
     6421 ttagggagag aaccatgctg ttacacattt tgtatttggt tggcatcact gccgaagcca
     6481 tgacgggggc gctggcggcc ggacgacggc gcatggatac atttggcgta attattattg
     6541 cgaccgccac cgcaattggc ggagggtcag tgcgcgatat tctgctgggc cactatccgc
     6601 tcggctgggt caaacacccg gaatatgtga ttatcgtcgc taccgccgca gtactcacca
     6661 ctatcgttgc tcctgtaatg ccttacctgc gcaaagtgtt tctggtgctc gatgcgctcg
     6721 gactggtggt cttttctatc atcggcgcac aggttgcact ggatatgggg cacggcccaa
     6781 ttattgccgt tgtcgcggcg gtaaccaccg gcgtgtttgg cggcgttttg cgcgatatgt
     6841 tctgtaaacg catcccactg gtattccaga aagagttgta tgctggtgtc tcatttgcct
     6901 ccgccgtgct gtacatcgcg ctgcaacact atgtttctaa ccatgatgtg gtgattatct
     6961 ccaccctggt attcggcttt tttgcccgct tactggcgct acgtcttaag ctgggattac
     7021 cggtctttta ctacagccac gaaggtcact aaggttcaaa acctgtgatc tgctgggcag
     7081 ccagccaact gcccagcttc ttgatttgcg cattttcctt ccattcaata acctgtctgg
     7141 cgcgcccaga tccagtcccc ggcagctgct gtcagaactg ctccgtgcta aataaaagtt
     7201 gcgactagga ccgttcatca ctggcattaa gcgccgcccg ggttagcggt attcccattg
     7261 ccatcaccca gcgagtaaaa ggctgcttac gagccagatt aaactgatgc cataactgcg
     7321 cacttttact tttcgcgatc cccggcgtgt tctgtaattg ctctggcgtt aataaaagcc
     7381 aagaaaagat atgctcaaag cgatgagtct gatgcagcgc gcgccaaccg gcctcaccaa
     7441 tgccatccag cccaagaacc tgttttgacc ccag
//
LOCUS       DAJTYD010000179         7308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTYD010000179 DAJTYD010000000
VERSION     DAJTYD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.179"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            149..265
                     /locus_tag="OO585_003434"
     CDS             149..265
                     /locus_tag="OO585_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6630907.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            629..1471
                     /locus_tag="OO585_003435"
     CDS             629..1471
                     /locus_tag="OO585_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6630908.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1474..2562
                     /gene="capU"
                     /locus_tag="OO585_003436"
     CDS             1474..2562
                     /gene="capU"
                     /locus_tag="OO585_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6630909.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2567..3517
                     /gene="virK"
                     /locus_tag="OO585_003437"
     CDS             2567..3517
                     /gene="virK"
                     /locus_tag="OO585_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6630910.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3582..4526
                     /gene="lpxM"
                     /locus_tag="OO585_003438"
                     /gene_synonym="msbB"
     CDS             3582..4526
                     /gene="lpxM"
                     /locus_tag="OO585_003438"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6630911.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4680..5192)
                     /locus_tag="OO585_003439"
                     /pseudo
     CDS             complement(4680..5192)
                     /locus_tag="OO585_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5223..6815)
                     /locus_tag="OO585_003440"
     CDS             complement(5223..6815)
                     /locus_tag="OO585_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6630912.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRYILSV
                     LPEWPSNRVDELLPWNVALTNK"
     gene            complement(6846..7196)
                     /gene="tnpB"
                     /locus_tag="OO585_003441"
     CDS             complement(6846..7196)
                     /gene="tnpB"
                     /locus_tag="OO585_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6630913.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(7193..>7308)
                     /locus_tag="OO585_003442"
     CDS             complement(7193..>7308)
                     /locus_tag="OO585_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6630914.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
BASE COUNT         1887 a         1625 c         1730 g         2066 t
ORIGIN      
        1 ttatcggtgg caatagcctg tgcaaatttt ttataacccg ggatcgtgtc aggattcaga
       61 gtatgataaa gacagatttt catggctgcc ggtttccgtc gtgcaaaaaa catgctgatt
      121 ttacgaagga atgccttact gaacaacctt gcccggaata ccccccgaaa tcagatggaa
      181 caatcagaaa aatgctctgc agttatggaa taaagaaggt ggggtggtac gccgggaggg
      241 cgatccgctg gaaatggcgc agtagctgca ggcctgtcat gatgccggaa tagaagttcg
      301 tgtacaggtt aatgaaagcg tcaccccgta aataaactct gcgctgaaat tccttttttt
      361 cattggagca cctgtgttgt tctgaggtga acatatcacc tctgttcagg tagccaaatt
      421 cagtgtgcca cctcaaggtt cttagtgagt cgtttttaaa agatatgata atatatcatt
      481 gtttatgaat tgttgatgtt taatttagta aaatatatca agtatgccat gggtttgcta
      541 tgttattcat ccagtatctg gaaataaaat gcaccagctt tattgggttt tccttttgtc
      601 tctgcttact aggtgccaga aactttaaat gttaaacgaa gggggcattt tgttcaaagc
      661 caatcatgtt ccggtattaa tgtatcatca tgtcagtcat tgccccggac tggttacact
      721 ttctcccgtg acattccgga agcaaatgaa atggttggct gaaaataact ggaaaacact
      781 gtcatcagat gaactggagt ttttttatcg gggcggaaaa ctcccccgga aaagcgttat
      841 gttgacattt gatgatggat accttgacaa ctggtttcag gtatatcctt tattaaaaga
      901 gtttaatctc aaagcacata tttttttaat taccggtttt attgggaatg gtccggttcg
      961 tcactctcct ggaaaggaat attctcatcg cgattgcgaa catcaaatag cgacagggaa
     1021 tgctgataat gttatgcttc gctggtctga agtaaacgaa atgttgcaat ctggtctggt
     1081 cgaatttcat gttcatacgc atactcatac acgctgggat aaaaagttca gttcacgaga
     1141 agaacagtgt aaacatcttc ggcaggactt gttgagtggg agagaatatt tgaaagaaat
     1201 gacaggaaaa tgcagtaaac atttgtgttg gccggaaggt tattataaca aggattatat
     1261 tcaggtcgct gaagaacttg gattctatta tttatataca acagaaagaa gaatgaatgc
     1321 tcctgctaaa ggaactacac ggataggaag aataagcacc aaagaacggg aaagttgcgc
     1381 atggctgaaa agacgacttt tttattatac aacaccgttt ttttcatcgt tgctggcctt
     1441 tcataagggg ccaagattac cggatgatta ataatgaata tactatttac ggaatcatca
     1501 ccgaatatag gtggtcagga attacaggct gttgctcaaa tgaaggccct gaagaaaatg
     1561 gggcattcag ttctgcttgt ctgcagggaa aacagcaaaa ttgcttttga agccagtaaa
     1621 ttgggaattg atatcacatt cgcgttattt cgaaacagtc ttcacatccc tactgcatgg
     1681 agattactcg gaatagttca tggttttcag cccaatgcaa tcgtttgtca cagtgggcat
     1741 gatagcaata ttgttggttt agtacggtta ttcacttgga aacatccatt cagaattatc
     1801 aggcaaaaga catatttgac acaaaaaaca aaagtttttt caataaatca tttttgcgat
     1861 gaggtgattg ttcccggaac aagtatgaag acacatctgg agcaggaagg atgtcgaacc
     1921 cgggttactg ttgtgcctcc aggctttgat ttccagaaat tatacgttga ttcccgaaac
     1981 agtttgcctc caaatgttct ttcttggctg gcgtcccgaa ggggatgccc cgttattgct
     2041 caggtaggaa tgttgcgccc ggaaaaaggg cacgaattta tgttgaattt actgttccat
     2101 ttaaaaatga atggacgaca gttctgttgg ttgattgtgg ggtctggttc gcctgaactg
     2161 cgggagcatt tacagtatca gattgacagt atgggcatgc atgatgatgt ttttattgct
     2221 gacaatgttt ttcctgccgc ccccgtatat cgggttgcca gtctggtggt tctgccttca
     2281 gaaaacgaat cgtttggtat ggtactggca gaagcatcgg cattttctgt gcctgtactg
     2341 gccagtcaga ttggtggaat ccctgatgtt attcagaaca accagaccgg gacattgtta
     2401 ccagcaggta ataagcacgc atggatgtgc gccctgaatg atttttttaa tgaccctggg
     2461 cgtttttatc agatggctcg ccaggcaaaa caggatatag aagagcggtt tgatattaat
     2521 aaaactgcgt taaaaatact cacattagcg aagcacaagt aacaatatgt tttctgtaag
     2581 taacttatca tttatcggtt tccttaaaag gattgttttt tcctcagatt cactcccggg
     2641 gaagtgggaa cacagaaaat tccggttcat gtacattttg cgatgtgcta taaatccggt
     2701 tgccagtatt cgatattatt acgaactgcg ttccttgccg tgcattgagg atattctggc
     2761 aatacacccc acgttgccag caagaattca tcgcccttat ttgcataaag gggggcgcgc
     2821 ctggacccgt ggacaatata ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa
     2881 atattctaaa tttttgtttc cacaaaaatc ggtatcatta gtccaattca tcgggaaaga
     2941 tggagagaat tttgatatcc agtgttcttc cagtggtttt gaccgggaag gagagttgat
     3001 gttgtcattg ttttacaata aaacagttat tacccggtta acattttcag tcattctgac
     3061 tcaaaatgga cacattgctt ttattggcgg attacaggga gcccctaaaa ataccggacc
     3121 tgatgttatt cggtgcgcaa cccgggcctg ttatgggatt ttcccaaaac ggattatttt
     3181 tgaagcattt tgtgcgttga tgaaagcttg caatatctct gagtgtctgg cagtcagtga
     3241 gcacagtcat gttttccggc aattgagata ctggtatcag aaacgcaaaa cctttgttgc
     3301 ggtctatagt gatttctggg agtctgtagc aggaaaaacc tgtggtgact ggtacagatt
     3361 accaacccag gtgattcgaa agccgctaag cgatatagcc agtaaaaaac gctctgagta
     3421 ccgaaaacga tacgctttgc tggattatat ccatgaaact actatccgct ctttggatgc
     3481 atatcctgta cactcagaac atcaggattt aaattaaggt ttgatgtatt ctctgaataa
     3541 aacagtaatg ataattttgg tagaggcatt cgcactaaat aatgaaaaaa tataaatctg
     3601 agtttattcc tgaatttaag aaaaattatc tttcccctgt ttactggtct acatggttcc
     3661 ttttgggaat gattgcaggt atttcaatgt ttcccccttc attcagagat cctgtcttgg
     3721 caaaaatagg gcgttgggcg ggcagattga gcaaaaaagc tcgtcgcagg gcgacgatta
     3781 atttatcgct ttgtttccca gaaaagagtg atacagaacg ggaaataatt gtcgacaaaa
     3841 tgtttgccac agcattgcaa tccatagtga tgatggcaga actggcaatt cgtggtccgg
     3901 aaaagttcca gaaacgagtg ttctggaaag gcctcgaaat tcttgaggaa attcgataca
     3961 ataacagaaa tgtgattttt ctggttcccc atggctggag cgtggatatt cctgcaatgt
     4021 tgctggcagc tcagggggaa aaaatggccg ccatgtttca tcagcaacga aatccagtga
     4081 ttgattatgt ctggaattct gtacggcgta aattcggggg gcgcttacat tcccgggagg
     4141 atgggataaa accatttatt cagtcagtac gccagggata ctgggggtat taccttccag
     4201 atcaggatca tggtcctgaa tacagtgaat ttgctgattt ttttgcgacc tataaagcga
     4261 cattaccaat tattggacgt ctgatgaaca tcagtcaggc tatgattata ccgcttttcc
     4321 cggtttatga tgaaaaaaaa catttcctga ctattgaagt tcggccacca atggatgcat
     4381 gcattgccag cgcggacaat aaaatgattg cccgacaaat gaacaaaaca gtggaaattt
     4441 tggtggggtc acatccggaa cagtatatct gggttttaaa attgttaaaa acgcgcaaat
     4501 caaacgaagc ggacccgtac ccttgaaatt ataaagtaca ggtatttcca ctagttgttt
     4561 cttacaggtt accaatcgaa acacatcccc cttccgtgtg ttgcccgttt ggcacggagg
     4621 actgtgtaga tgcagttcgt taaatttaat ttatgcctaa atagctgcgc ttaataccgc
     4681 tacacttttg ccagtccatg tttgcctccg gggaatgggc tgacggtttc cataaaatgg
     4741 cgaacttttt tcaacagttg ccacattgag cggcactgat gattacgcgt tattgtgtcg
     4801 tgaagtgcct gccatagccg ttcagcatga ttcacccatg gcgagtaaac cggctgataa
     4861 atgaccctga acttcgggtt ctccttcagc cagctctgtg tttcccggct tttgtggata
     4921 atgtagttgt ccacgatcag cgtgatggtt ttcgcccgac ggtatgtcgc tttaagccgc
     4981 ttcagcaggc tgatgaacag cgccgaactt ttgctgttgc cgcccacata gctgacttta
     5041 cctgtcccgc tgtgcagcgc tccggccaga taatattttt cattctgtcc cggcgtgacc
     5101 acccgttttt gctgtccgcg cagttgccag tccgcaccga ttttgggatg aagatggata
     5161 tccacttcat cttcataaaa gacctgatgt tcgtaagcgt caacggagca ccgtattgac
     5221 gcttatttat tggtgagtgc tacgttccat ggcaggagtt cgtcaacacg gttggaaggc
     5281 cattccggca gtacgctcag gatatagcgc agatacgctt ccggatcgat accgttcagt
     5341 cggcaggtgc cgatcagccc gtacagtagc gcaccacgct ctccaccgtg atcgctgccg
     5401 aagaacataa agtttttctt tccgagacag actgcacgaa gcgctctttc cgcggcatta
     5461 ttatccgcct cagccagacc atcatcactg aagttgcaca acgcaaccca gtgcttcctg
     5521 atataccgga acgcatctct cagacggcat tttttcgata acgtgtgttc tttctcctgc
     5581 atcagcttat acagggaagt cagtagcggt ttactctgca tttgcctgac cgccaggcgt
     5641 tcagacaccg gcaacccgcg tatttcgtgc tcgatggcgt acagctcacc gattagtttc
     5701 agggcttctt ccgctgtcgc gcttttggta ctgatatata catcgtggac tttgcgccgc
     5761 gcatgagccc agcatcctgc ttccgtcaac gcgccgcctt ctcgttcggc actgaacagc
     5821 cgatcgtaac cattaaacgc atctgcctgc aggatacccc ggaagggact aaggtgctgc
     5881 tccggatgtt taccctgatg gtccggtgag taggcgaacc agaccgccgg aggctctggc
     5941 gaaccggcat tcctgtcatc ccggacatac gtccagatat atcctgtttt cgccttcttc
     6001 ctgcccggtg ccagcacttt tactggtgtg tcatcagtgt gaactttgcg gctgttcatt
     6061 acataacggt acagagcatc attcagcggc gtcattaact ggcagcacgc gtcaacccag
     6121 ttggagagta atgcacggct cagttcgaca ccctgacggg caaaaatttc actctgacga
     6181 tacagtggca ggtgttcgca gtattttccc gttaacacgc gggcaagtaa ccccgggccc
     6241 gcgataccac gctctatcgg acgggatggt gccggtgctt caacgatgca gtcacatttt
     6301 gtacaggctt tttttacccg ttctgtgcgg atcactttca gagcgctgct caccagttcc
     6361 agttgttctg cgctgacttc ccccagataa tccagctcac cgccacactc cgggcaacag
     6421 ctttcttctg gctccaggcg atttatttcg cggggcagat gctccggtaa cgggcgacga
     6481 tgacgagact gtcgcaactg gcggggaacc tgcggatcgt cttcccgccc actgtaacga
     6541 tcactgtcct gttcgcgttg tttcagcagg gcctcagcct gttcaacttc acgacgcagt
     6601 ttttcagaac gggtaccgaa cagcatccgg cgcagttttt ctatctgagc ccgcagatgt
     6661 tctatttccc gttcatcttc ttcgatcttt tcttcggcgc gggccagtgc agagcgcagg
     6721 aaggcctccg tctcttcaac cagactcagt tgctggtctt tctgacggag ctggcattcc
     6781 agttctgcaa tgcgaatgag gtatttttga ttcatggccg tttttataat ccggccatga
     6841 catttttaca acattgtcag tgcattaagg cgggatgttt ttggctgacg ccagtccagc
     6901 ttatcgagga gcattgccag ttgcgagcgg gtaatggata ccttgccgtc acgcacagca
     6961 ggccagataa actggccttc ctccaggcgt ctggtgaaca ggcacagacc atcagcatca
     7021 gcccacagga ttttaatcat gtcaccccgt cggccacgga agatgaacag gtgaccggag
     7081 aagggattat cattcagcac atgctgtacc tgttcaccca gcccgttgaa agacttacgc
     7141 atatcggtta tcccagcaac gagccagatg cgggtgcctg acgggagtga gatcatcgtc
     7201 ccctcccggt cagttcacgg atcaacactg tgagcagctc tggtgaagga ttttccagcg
     7261 tcatgttacc gtgacggaac tccaccttgc aggagctggc actaactg
//
LOCUS       DAJTYD010000180         7255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTYD010000180 DAJTYD010000000
VERSION     DAJTYD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.180"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..173
                     /locus_tag="OO585_003443"
     CDS             <1..173
                     /locus_tag="OO585_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HCR6630915.1"
                     /translation="LTGNSSVMGVHIESAARHPSTIGVAVSTGCWAHRRGTLLVHADL
                     TFENLSHTRSAL"
     gene            170..775
                     /gene="ttdB"
                     /locus_tag="OO585_003444"
     CDS             170..775
                     /gene="ttdB"
                     /locus_tag="OO585_003444"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6630916.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            824..2287
                     /gene="ttdT"
                     /locus_tag="OO585_003445"
     CDS             824..2287
                     /gene="ttdT"
                     /locus_tag="OO585_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6630917.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(2330..3343)
                     /gene="tsaD"
                     /locus_tag="OO585_003446"
     CDS             complement(2330..3343)
                     /gene="tsaD"
                     /locus_tag="OO585_003446"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6630918.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            3580..3795
                     /gene="rpsU"
                     /locus_tag="OO585_003447"
     CDS             3580..3795
                     /gene="rpsU"
                     /locus_tag="OO585_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6630919.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            3906..5651
                     /gene="dnaG"
                     /locus_tag="OO585_003448"
     CDS             3906..5651
                     /gene="dnaG"
                     /locus_tag="OO585_003448"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6630920.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            5846..>7255
                     /gene="rpoD"
                     /locus_tag="OO585_003449"
     CDS             5846..>7255
                     /gene="rpoD"
                     /locus_tag="OO585_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6630921.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQM"
BASE COUNT         1717 a         1948 c         1948 g         1642 t
ORIGIN      
        1 ggctgactgg caacagttca gtgatgggcg tacatatcga atctgccgcc cgccatccgt
       61 caaccatcgg cgttgctgtc tctaccggct gctgggcgca tcgtcgcggc acactgctgg
      121 ttcatgccga tctcaccttt gaaaatctgt ctcacacccg gagcgcgtta tgaaaaagat
      181 cctgacaacc ccgatcaaag ctgaagatct gcaagatatt cgcgtcggcg atgtgatcta
      241 cctgaccggt acgctggtga cctgccgtga cgtttgtcac cgccgcttga tcgaactgaa
      301 acgtccgatc ccttacgatc tcaacggcaa agcgattttc cacgctggcc ccatcgtgcg
      361 caaaaacggc gataaatggg agatggtttc cgttggcccg acaaccagta tgcgtatgga
      421 aagttttgaa cgtgaattta tcgagcagac cggcgtgaaa ctggtggttg gcaaaggcgg
      481 aatggggccg ctgaccgaag aaggctgcca gaaattcaag gcgctacatg tgattttccc
      541 ggcaggctgc gcggtgctgg cggcaaccca ggtggaagag attgaagaag tgcactggac
      601 agagctcgga atgccggagt cgctgtgggt ctgccggggc aaagagttcg gcccgctgat
      661 tgtctctatt gatacccacg gcaacaatct gatagccgaa aacaaaaagc tgttcgccga
      721 acgccgcgat cccatcgtgg aagagatctg cgagcacgtc cattacatca aataaccctc
      781 ccggagaggc tcgccccctc tcctttttcg caggcataac acgatgaaac cttccactga
      841 atggtggcga taccttgcgc cgctggcggt catcgccatt attgctctaa ttccggttcc
      901 cgcagggctg gagagtcata cctggctcta ctttgccgtt tttactggcg tgatcgttgg
      961 actgatcctc gaacccgtgc cgggtgccgt ggtggcgatg gtgggtatct ccatcatcgc
     1021 catactctct ccctggctgc tgttcagccc cgaacagctc gcccagccag gctttaagtt
     1081 caccgccaaa tccctctcct gggcagtttt tggtttttct aattcggtta tctggctcat
     1141 tttcgccgcc tttatgtttg gcacaggcta tgaaaaaacc gggcttggac gccgcattgc
     1201 gctgattctg gtgaaaaaga tgggacatcg cacgctgttt ctcggctatg cggtgatgtt
     1261 ctccgagctg atcctcgcac ctgtaacacc gtccaactcg gcgcgtggtg cggggattat
     1321 ctatcccatc atccgtaacc tgccaccgct ctatcaatca caaccaaacg acagcagttc
     1381 gcgcagcatt ggctcgtaca tcatgtggat ggggattgtt gccgactgcg tgaccagcgc
     1441 cattttcttg acggcgatgg caccaaactt gctgttaatt ggactgatga aaagcgcatc
     1501 tcacgccacg ctgagttggg gcgactggtt cctcgggatg ttgccgctca gtattttgct
     1561 ggtgctgctg gttccctggc tggcttatgt gctgtacccg ccggtactga agtctggcga
     1621 tcaggtgccg cgctgggcag agacggaact gcaggcaatg ggcccgctct gttcgcgtga
     1681 aaaacggatg ctggggctga tggtaggcgc gctggtgctg tggattttcg gcggtgatta
     1741 tatcgatgcc gcgatggtcg gttacagcgt ggtggcactg atgctgcttc tgcgcattat
     1801 cagttgggac gacattgtca gtaataaagc ggcgtggaac gttttcttct ggctggcctc
     1861 gcttatcacc ctcgctaccg gactcaacaa cactggtttt attagctggt ttggcaaact
     1921 gttagcaggc agcttaagcg gttattcgcc aacgatggtg atggtggcgt tgattgtggt
     1981 gttttatctg ctgcgctact ttttcgccag cgccacggcg tatacctccg ccctcgcacc
     2041 gatgatgatt gccgctgcgc tggcgatgcc ggaaatcccg ctgccggtgt tctgcctaat
     2101 ggttggcgcg gcaattggtc tggggagcat tcttacacca tacgccaccg gacccagtcc
     2161 gatttactac ggtagtggtt atctgccaac ggcggattac tggcgactgg gggcgatttt
     2221 tgggctgata ttcctcgtgt tgctggtgat taccggctta ctgtggatgc ccgtggtgtt
     2281 gctttaagtg aggacggtgc cggaaattgc tctccggcac ccaatagttt tacgcagccg
     2341 gtaactccgc cagcggccag cgcggacgca cgctaacgcc gagatccgcc gtcgcgcctg
     2401 ctttaaaccg caccattccg gcataggcga tcatcgcgcc gttatcagta caaaactccg
     2461 gacgcgcgta gaacacttcg ccgcggcgtt ttttcatcat ttccgccagc ttcgcccgta
     2521 acgtgcggtt agcactcacg ccgcccgcca tgaccagtcg cttaaagccc gtctgatcca
     2581 acgctcgctt acacttaatc atcaacgtat cgaccaccgc atcttcaaag gcgcgggcga
     2641 tatcagcacg cgtctggtcg tcggtgccgt tgtcacgaat cgtgttcgcc gcaaaggttt
     2701 tcagaccaga aaagctgaaa tccagccccg gacggtcggt catcggacgc gggaaaacaa
     2761 agcgcccggc agtaccctga gccgccattt tcgacagtaa cggtccgcca ggataatcca
     2821 gccccagcag cttcgcggtt ttatcaaacg cttcaccggc ggcatcatcg atagactcgc
     2881 cgagcagctc gtactgacca atgccagtca cgctgattaa ctgcgtatga ccgccggaca
     2941 ccagcagcgc gacaaacgga aattccggcg ggttatcttc cagcatcggc gctaacagat
     3001 gcccttccat atggtgtacc gggattgccg gaacgttcca ggcaaacgcc agagaacgcc
     3061 ccacggttgc gccaaccagc agtgcgccga ctaatccagg gcctgcggta taggccacag
     3121 catcaatgtc ttttgccgtt aagccagact cctttagcgc cgcctggatc aacggtacgg
     3181 ttttgcgcac gtgatcgcgg gaggccagtt caggcacgac gccgccgtag tcagcgtgca
     3241 atttcacctg actatacaat tggttggcta acaaaccttt ttcatcgtcg taaatggcga
     3301 tgccggtttc atcgcaggaa gtttcaatac ccagtacacg catgacttgt tttacctcgc
     3361 tttattaccg cgcagtgtag gaccaatgcg ggttgatgta aaactttgtt cgcccctgga
     3421 gaaagcctcg tgtatactcc tcacccttat aaaagtccct ttcaaaaagg ccgcggtgct
     3481 ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga
     3541 tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat taaagtacgt
     3601 gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt
     3661 gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc
     3721 gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc
     3781 actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt tgtagttgta
     3841 aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc taaaaatcgg
     3901 ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctactggca cgcactgaca
     3961 tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt
     4021 gttgtccatt ccacaacgag aaaaccccgt cattcaccgt taacggtgag aaacagtttt
     4081 accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg aactacgaca
     4141 agcttgagtt cgtcgaaacg gtcgaagagc tggcagcaat gcacaatctt gaagtgccat
     4201 ttgaagcagg cagcggcccc agccagatcg agcgccatca gaggcaaacg ctttatcagt
     4261 tgatggacgg tctgaatacg ttttaccaac aatctttaca acaacctgtt gccacgtctg
     4321 cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgctcgc tttgcgattg
     4381 gttttgcgcc ccccggctgg gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc
     4441 gccagtcatt gattgatgcg gggatgttgg tcactaacga tcagggacgc agttacgatc
     4501 gtttccgcga gcgggtgatg ttccccattc gcgataaacg cggtcgggtg attggttttg
     4561 gcgggcgcgt gctgggcaac gataccccca aatacctgaa ctcgccggaa acagacattt
     4621 tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac gctgaaccca
     4681 atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa tacggcatta
     4741 attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc
     4801 gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgt gatgccgcat
     4861 ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg ccgtcagcta cgctttatgt
     4921 ttttgccgga tggcgaagac cctgacacgc tggtacgaaa agaaggtaaa gaagcgtttg
     4981 aagcgcggat ggagcaggcg atgccactct ccgcatttct gtttaacagc ctgatgccgc
     5041 aagttgatct gagtacccct gacgggcgcg cacgtttgag tacgctggca ctgccattga
     5101 tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat
     5161 taggcatact tgatgacagc cagcttgaac gattaatgcc aaaagcggca gagagcggcg
     5221 tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg tatacttata gggttgctgg
     5281 tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat gaaaataagc
     5341 tccctggact tggcttattc agagaactgg tcaacacttg tctctcccag ccaggtctga
     5401 ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc cttgaaaaac
     5461 tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc accgactcac
     5521 tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc gctcgtgagc
     5581 gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac caggagctgg
     5641 cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc cgacagccgc
     5701 actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg cggtaaacaa
     5761 ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa caccaacctc
     5821 atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc tgaaacttct
     5881 tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg accatctgcc
     5941 ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca acgacatggg
     6001 cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg aaaacaccgc
     6061 ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg
     6121 gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac
     6181 ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc aggttcaatg
     6241 ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg atcgtgttga
     6301 agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga acgcagaaga
     6361 agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag atctggacga
     6421 tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg acaacagcat
     6481 cgacccggaa ctggctcgcg aaaaatttgc ggaactacgc gctcagtacg ttgtaacgcg
     6541 tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct caggaagaga tcctgaaact
     6601 gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc tggtcaacag
     6661 catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt
     6721 tgagcagtgc aaaatgccga agaaaaactt cattaccctg tttaccggca acgaaaccag
     6781 cgatacctgg ttcaacgcgg caattgcgat gaacaagccg tggtcggaaa aactgcacga
     6841 tgtctctgaa gaagtgcatc gcgccctgca gaaactgcag cagattgaag aagaaaccgg
     6901 cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc
     6961 ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt ctggttattt ctatcgccaa
     7021 gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggca acatcggtct
     7081 gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca cctacgcaac
     7141 ctggtggatc cgtcaggcga tcacccgttc tatcgcggat caggcgcgca ccatccgtat
     7201 tccggtgcat atgattgaga ctatcaacaa actcaaccgt atttctcgcc agatg
//
LOCUS       DAJTYD010000181         7214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTYD010000181 DAJTYD010000000
VERSION     DAJTYD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.181"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            599..883
                     /locus_tag="OO585_003450"
     CDS             599..883
                     /locus_tag="OO585_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6630922.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            871..1356
                     /locus_tag="OO585_003451"
     CDS             871..1356
                     /locus_tag="OO585_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6630923.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1390..2568
                     /gene="ltrA"
                     /locus_tag="OO585_003452"
     CDS             1390..2568
                     /gene="ltrA"
                     /locus_tag="OO585_003452"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6630924.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2651..2805
                     /locus_tag="OO585_003453"
                     /pseudo
     CDS             2651..2805
                     /locus_tag="OO585_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2859..3341)
                     /locus_tag="OO585_003454"
                     /pseudo
     CDS             complement(2859..3341)
                     /locus_tag="OO585_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3597..4577)
                     /locus_tag="OO585_003455"
     CDS             complement(3597..4577)
                     /locus_tag="OO585_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6630925.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4577..5776)
                     /locus_tag="OO585_003456"
     CDS             complement(4577..5776)
                     /locus_tag="OO585_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6630926.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6098..6436)
                     /locus_tag="OO585_003457"
                     /pseudo
     CDS             complement(6098..6436)
                     /locus_tag="OO585_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6503..>7214)
                     /gene="istB"
                     /locus_tag="OO585_003458"
     CDS             complement(6503..>7214)
                     /gene="istB"
                     /locus_tag="OO585_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6630927.1"
                     /translation="AAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTRMAA
                     FPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYE
                     AVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQKEA
                     KLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGES
                     YRLRQKRKAGVIAEANPE"
BASE COUNT         1918 a         1644 c         1728 g         1924 t
ORIGIN      
        1 gcggcatccg gcatataggc agcatgaacg caacacaggc tctcgtacgg aacgtgggaa
       61 cctgtcgtct cgatgctaag ggagcggcgc ggagcagagg cactgcaagc gcctgagtac
      121 cgatgcgaga cacaggggca gatggcttcg tagtagtgag gaagtgcctg taatgggcat
      181 ggagcgaagg gggtcagtcg aggcgtttga ggttaccaat cgaagctata cccggtaatg
      241 gcgcaggttg acctgcactt ggccaagtgg attgcccgca aacacaagcg agcgcgaggt
      301 agtctggtgc gtgcattcga gtggttactg cgaattcgtc acgactgccc gacgctgttt
      361 gcacactggt gcttagctta cgatacttga atggttcaac gacaagagcc gtatgacgcg
      421 agagtgtcac gtacggttcc gtgggagcgt aggggggcgg tgcccctgcg ccacccgata
      481 tcccctttga gtgcggttac gtccacagag gtaaatgccg ctgacgcggc atctgtaatc
      541 tgttactttg tgtacatcat ttgctatata ttgtcaatat gctattgttg taaagcaaat
      601 gatatacgga ggctttatga aatcaggtgt tcagctcaat ctcagagcaa gagaatcaca
      661 gcggatactc attgatgctg ctgcagaaat tcttcataag tcgcgtacag atttcattct
      721 ggaaatggcc tgtaaagctg ctgaagatgt tattctcgac cggcgggtgt ttaattttaa
      781 tgacaggcaa tatgaagagt tcattgaaat gcttgatgct ccggttgcag atgatcctgc
      841 catcgaaaaa ttgctggcaa ggaaacctca atgggatgtg taactgcacc agaaccttta
      901 tcctcatttc atcaggtcgc tgaatttgtc agcggtgaag ctgtacttga tgactggtta
      961 aagcagaagg ggctaaaaaa tcaggcaatc ggagccaccc ggacttttgt tgtatgcaga
     1021 aaaggtacgc aacaaatagt cggtttttac tcactggcca ctggtagtgt caatcataca
     1081 gaagctacag gtaaccttcg gcgcaatatg ccagacccca ttcctgtaat tatacttgca
     1141 cgcctggctg tagatgtgtc atttcgagga aaagggcttg gcgcggattt acttcatgat
     1201 gcagttcgtc gttgttacag ggttgccgaa aatatcggtg tgcgtgcaat tatggttcat
     1261 gcgcttacgg aaaacgcaaa acagttttat attcatcatg gttttaagcc atcaaaaact
     1321 caggtgcaaa cattgtttct taaactccct caatagggag taatgcactg gtctgggcat
     1381 catatctgga tgtgcgtcgc aacaagggag ccccccggat gcgacgggca gactttgaaa
     1441 atgttcgacc agcaacgtga tggcaatcta tacaagatct ggaaccggct atgttcggga
     1501 acatggtttc cgccaccagt gctggaaaaa cggatcccga agtcgaatgg caaggaacgc
     1561 attctgggga tcccaacagt atccgatcga attgctcagg gagcgataaa acttttcatg
     1621 gaagaaaagc ttgacccgat tttccatgct gattcatatg gttaccgacc aggcaaatca
     1681 gctcatgacg ctctgaaaca atgtgccatc cggtgctggc gttatagctg gatactggaa
     1741 gtggacatca gtgcgttttt cgaccatgtg aaacatgatc tggtgctcaa agctctggaa
     1801 caccacggga tgcctaaatg ggtcatcctg tactgtcgac gctggatgga agcgccaatg
     1861 cagagttgtg aaaatggaga attaataacc cgaacacgag gcactccgca gggcggggtc
     1921 attagtccgc tacttgctaa cttgttcctt cactatgcat ttgatttgtg gatggaaaga
     1981 gaatatcggg gggtaccgtt tgagaggtac gctgacgata ttgtagtaca ttgttcacga
     2041 atgagtgatg caacaaggct aaagaacagg ttatcggagc gcttctcgga agtcgggctg
     2101 gtcctgaatg cagggaaaac gaatatcgcc tacattgaca cgtttaaaag gagaaacgtc
     2161 gcaacgagtt tcacctttct cggatatgac ttcaaagtgc gtacgctgaa gaatttcaaa
     2221 ggcgaacggt accgaaaatg catgccgggt gcgtcaaatg cagcaatgcg caaaataaca
     2281 gaaacaatca agaagtggcg tatacatcgc tcaacagctg agagtttgct ggattttgcg
     2341 aggcgctaca atacgatagt gagaggctgg atcgagtact acggaaagtt ctggtccaga
     2401 aatttcaact atcgactgtg gagtgcaatg cagtcacgtc tgctcaagtg gatgcagtct
     2461 aaatacagac tttcgaaccg gaaggctcag cgaaagctga cgctggtaag gaaggagtat
     2521 ccgaagctat ttgttcactg gtatttacta cgtgcatcaa atgagtgatc aagagccgta
     2581 tgacgcgaga gtgtcacgta cggttccgtg agcagccggg gagtgaaatt ctctccggct
     2641 gactcgactc gcgggttccg acagcgatga cgaacatgcg gcggtgttgt actcgctgat
     2701 cggcacatgc cgtctgaaca atgtggaacc agaaaatggc tgcgttacgt cattgagcat
     2761 atccaggact ggccggcaaa ccgggtacgt gatctgttgc cctagaaagt tgatctgagc
     2821 tctcagtaaa tatcaatacg gttctgacga gccgcttact tttggcgtaa ttctgctgcg
     2881 cctttactga acacaatgcc gtattcagca agaagtccac gaatgtgatt tatcagttgt
     2941 gtcctttcca ttgaaaccaa ccgacgcata ttcaaaaaag tttggacgtc ctgttgctcc
     3001 agagttttta ttggtacggg tcgtatagtt ccatgttggc aggcctcagc tattgcctgt
     3061 gcgtcgttat aatcattttt ctggcaccgc tgataagctt taacatgttg tgccggaagc
     3121 aatattacat catggtcgag tttcctgatt tcccgggccc agtaatgaga tgccccgcca
     3181 gcctccatcg caacagtcat tttaggctgt tgcaccagcc agttaagcag ttgattgcga
     3241 gaaagtttgg tctttcctgc gggtttgcca tgagcattga tactgaaaag ataaaagttc
     3301 gttttagcca gatcaatgcc ggtgattttc tgttcagaca tggtatcccg cttacttttg
     3361 tggttaaagt ttatccatca tggcgcaatt gaaggctgcc gtgaaagagg gggaagacta
     3421 tctcatcggt tactgttaaa cggctaagag aaactatctt tttcaagcaa agaagatacg
     3481 gtgaaacagg cgatttcacc gtgaaatact gttcaaaaaa aacgctaata aaagcaaaat
     3541 ttcttttaaa acagccaatt aaaaactggt gagttgctgg cgaaaaactt gaattttcac
     3601 agcgaattca gattatcttt tagtacaaga gcaatcatac gatctaactt atcctgaact
     3661 tccaacggta atctgccgaa ttcatatgag aacgtacgtc cctttactcg ttttcgagca
     3721 aatttatctt tactatcaaa tttccataac aaagttacaa ccgctttatc ctttactcct
     3781 gaatcaagca atgaagacat ttccttggtt ataagccgca taatactgtt tttaacctcg
     3841 tcttcagaaa gattgtcatc agcagtaata agctcaatac tcggcgagat attcttcaca
     3901 acttcatcaa tagatatatc acccagcctc agtcgttcac ttaattcagc cagttgcctg
     3961 taatcggcgt atgtcagctc agactgaaca ggaaaaagag acacaaaatc tttaggtacg
     4021 gacgctgctt ggagggcacg agtcacctta gctgccgaca atccctctct ttctgcaatt
     4081 tgcttctggt tcataccggc ctctttgaga cgtactaaac gcaacccaat ttcacgaata
     4141 ttatgttcaa gtgacgtctg caaatctttc gccagatgct gagcttcaga aacagtaagc
     4201 tcatcatccg ttacaagtac acgtaatcca accttgcata gcaaagctgc tgcccgacga
     4261 cgggaaccat ccaaaatctc aataagatcg ccagtccgaa tcccaataca tggatagaat
     4321 tgttgtaaac gtatagttct ggtgatgtct tttagtgagt ccggcgtcag ggctgtctgt
     4381 tcgcggccat tcacctcttc aaccacaaaa gtttgagtgt ccactttgtc atgatcaacc
     4441 ttgatctcag taaattttgc ctttcggcca gtattgaggg taaatacatg aactggagcc
     4501 gatatttctt cagtattatt caggatattt gtattcagcg tacggccaat agtcggtcgg
     4561 tgctttctgt tttccatcag tagttggccc ttataaattc aatacgatca aaaactgctt
     4621 tagcgaaatc ttctgctgcc atacgtgcat ttttcaaagc ttcaccactg ccaacataag
     4681 taacaggatt cgcagatatc acggtatcaa acgactcgcc gcttctctca aaaccatcaa
     4741 gacgtggcat tgaaacatca agcatgtcac caccgaaaat ctcttttgtc aggctatggc
     4801 agtatttatg atctgactta ttcgccagtt tcgacatgaa gccaatattt gcctgcaaac
     4861 ggcatgaaca tccatcctgt tcaataatct gtacaagttc aggtaaacga gccaaatatt
     4921 taagcgtgga atggaaatca acttgtgctg gcggcaccgg tgtaaacatg atatctgcgg
     4981 cagcaatggc atttttcaaa aatgcgtcca gatgcgggcc tgtatcaata aggatgaagt
     5041 cgaaatcatg cttcagctta tcaataatat tctcacgcaa tatcgcgtgt ttgttttgtc
     5101 ctaatagatg ctcctcgcac agagtatccc agtttgatgc aataaaggcg tcgtcaatag
     5161 aagccggcat aacataaacg ccagggataa ctgacggaac gataaaatct tccagtaatt
     5221 cctcacgact cacgttctgc aacatagcct gcggtgcggt tgtatcaacc agccccaccg
     5281 catgcaagta gttcagaaac atagtggctg atgactgggg atctaaatca aggagcagaa
     5341 tacgtaaatc ttctgacagc aggtgaggat gagcacgcag ggcgtgagct acggaaacac
     5401 tactcacggt tttagacaca ccgcctttta gactaccgat gaatatggaa tatgcctcac
     5461 tataacgatc gcgatattta ggaatgccac gatgtgcata gatatcgata atgttctgga
     5521 tggttaatgc atattttgtc gcggttcctg ctgggcgttt ttcaaactga tagccctgag
     5581 cctccatctc accgactgca ccatccacgc ttgcgcgcgt taattttgga agcttagcta
     5641 cagcagactt tgaatagact tgataaaaaa catcagcctg aaactcctgc ttttgctcct
     5701 gaatctgttt tgttagggcg agcaacattt tatctgcacg ctgcgcaacc ttacttaact
     5761 gctcaaaact agtcattccc cctccgtgct atgtaggttt ttaacttatt acaaaaaaaa
     5821 ctgcattagc agggtaatgc atgcaagtgc atattgtaca tgcttatatt aaaaatgcgc
     5881 gcattgctga tttattaatc atcggaatga atattactgt aagatagtat gcctttccgg
     5941 gagaaagacg ctgatttccc ccgaataatc cagttcacca ccacactccg gacaacagct
     6001 ttcttcaggc tccagatggt gtatttcgcg ggaaacatgt tccggtaacg gacggcgatg
     6061 acgtgactgt cgcaacaggc gggggtagtg cttccaatca gtagaacatg tgcttttcaa
     6121 tgaaggagcc gatgactttt tcgtggattt ccacggaacg cgagaagcag attgttttac
     6181 gtgccagccg cttgatgcgg gtgcggaggg tccggttgtt acgttcaata cgttgcgtga
     6241 agattttgcc ggtcaggtgt ttctccttcg gcacttctct ggcgtagctg ccccagtcat
     6301 cgctggtcac catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct
     6361 catcattccg tggcccgaaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat
     6421 accagagcca gtgttgtgtc aacgacggat gaaaagtgat ccacttatat ctccaccaac
     6481 ggcccaatat tgatccaccg ttttactcag gattagcttc tgctataacc ccggcctttc
     6541 gtttctgtct gagtcgatag ctttctcctt tgatttgaac gacatgtgag tggtgtaaga
     6601 tacggcccag catcgctgag gtcagtgctg catcaccggc gaacgtttga tcccactgcc
     6661 cgaacggtag attggatgtc aggatcattg cgctcttttc ataacgttta gcgatgacct
     6721 ggaagaacag ttttgcttct ttctgactga acggcagata gcctatttca tcaatgatga
     6781 gcaggcgggg ggccattact ccacgctgaa gcgtcgtttt ataacggccc tgacgttgtg
     6841 ccgtagataa ctgaagtaac agatctgctg ctgttgtgaa gcgaacttgg atacctgcac
     6901 ggactgcttc atagcccatc gcgattgcca gatgggtttt ccccacacct gatggcccca
     6961 gtaatacgat attttcatta cgttctatga agctgagtga gcgtaacgac tggagttgct
     7021 tctgcggtgc tccggtggcg aatgtgaagt catactcttc gaacgttttc accgccggga
     7081 aggctgccat tcgggtatac atcgcctgtt tacgttgatg acgtgccagt ttttcttcat
     7141 gaagcagatg ctccaggaag tccatataac tccattcctg gtctactgcc tgttgtgaca
     7201 gcgcaggcgc tgcg
//
LOCUS       DAJTYD010000182         7203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTYD010000182 DAJTYD010000000
VERSION     DAJTYD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.182"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(235..789)
                     /locus_tag="OO585_003459"
                     /pseudo
     CDS             complement(235..789)
                     /locus_tag="OO585_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            932..1054
                     /locus_tag="OO585_003460"
                     /pseudo
     CDS             932..1054
                     /locus_tag="OO585_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OO585_003461"
     CDS             complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OO585_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6630928.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2164..2478)
                     /locus_tag="OO585_003462"
     CDS             complement(2164..2478)
                     /locus_tag="OO585_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630929.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2556..2666)
                     /locus_tag="OO585_003463"
                     /pseudo
     CDS             complement(2556..2666)
                     /locus_tag="OO585_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OO585_003464"
     CDS             complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OO585_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6630930.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGVPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4038..4175)
                     /locus_tag="OO585_003465"
                     /pseudo
     CDS             complement(4038..4175)
                     /locus_tag="OO585_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4257..4555)
                     /locus_tag="OO585_003466"
                     /pseudo
     CDS             complement(4257..4555)
                     /locus_tag="OO585_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5144..5303)
                     /locus_tag="OO585_003467"
                     /pseudo
     CDS             complement(5144..5303)
                     /locus_tag="OO585_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5307..6461)
                     /locus_tag="OO585_003468"
                     /pseudo
     CDS             complement(5307..6461)
                     /locus_tag="OO585_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OO585_003469"
     CDS             complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OO585_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6630931.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6828..>7203)
                     /locus_tag="OO585_003470"
     CDS             complement(6828..>7203)
                     /locus_tag="OO585_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6630932.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
BASE COUNT         1855 a         1678 c         1560 g         2110 t
ORIGIN      
        1 aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga cgatctgagt
       61 ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg gataactttg
      121 cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa acaatatgct
      181 cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac aggcctaagc
      241 gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt gccgtcgcca
      301 gcgattgtaa tcacattcga tataattaaa cactgttgcc cgcattattt cccggctgat
      361 aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt ccacgcaggc
      421 attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc acttcagcag
      481 cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga taacgttccg
      541 gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt gcgccgtcag
      601 gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga ccacagccag
      661 atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact tctggttcgg
      721 accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc catgtgcgcg
      781 gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact acgtgcatcg
      841 aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt gagcagccgg
      901 agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg acgaacatgc
      961 agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc cagaaaaatg
     1021 gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac gcgatctgtt
     1081 gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac gagccgctta
     1141 cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa atacaaaagc
     1201 tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat agcaccgctc
     1261 aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc tgtcatcaga
     1321 aaggcgattc taaatttgta atataatcac tcatattccc acgtaatttc tttagatcaa
     1381 tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca ttgcttgttg
     1441 tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc tttttgaaat
     1501 aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg tctagagaaa
     1561 tgtgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg ccgatttttc
     1621 ggctgtgttc tgcacaaaac ttatcaatac attggtttac cacccgggat acgtagactt
     1681 ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa acagagctca
     1741 aaatactagt atttattttg gttgatgata tttttactat ccttttcccc attgtacagc
     1801 ggataatctc atcaaccttt cctgaaaaat atcttttgtg agataaactg attccaaaat
     1861 tattaaaaga tgttccaagc attataatca ctttgtcatg taaaatagta acattgtcat
     1921 tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc cttggctcaa
     1981 tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta tgaaaaaact
     2041 tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc attcataatg
     2101 atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt cgtagtgtca
     2161 gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct tcacgacatt
     2221 cgtgttgagg ctattcatga aaactccacg ctgaaaacgc ctttttgtat aaacggtcac
     2281 ggtatgcccg gagccactgt tgctgatgaa ttcgataaaa ccttggcgtt tcttgaagct
     2341 atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc cgacaccctg
     2401 ggcgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc caaccttgcc
     2461 tctctggcgc tggagcatga agtgatttag ctgggacgta gccactgaca gcttctataa
     2521 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
     2581 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
     2641 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
     2701 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
     2761 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
     2821 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
     2881 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
     2941 tcggggagag ctccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
     3001 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
     3061 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
     3121 cgatattccc tttccctgcg gccagaaata atcctggtac cccctcatta tctcttacat
     3181 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
     3241 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
     3301 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
     3361 attcaccgta tttacgcgct gcatcagcat ttccatgcca aatcaatgaa aacaaattat
     3421 ggcggttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
     3481 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     3541 tagcggtatt tgtcacgtta atgatattac gtttcattcc tttattttca ttagcagtat
     3601 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     3661 ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt gtaatgtaat
     3721 agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc tatattcacc
     3781 aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc cctaagattc
     3841 aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg accgtcaagg
     3901 cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt aaggggcgtt
     3961 gtccatttct gtgatgctgc ctagatttaa ctgcctgctc cggcttatct acatctaaat
     4021 atgtgagcct aataccgcta cactttggcc agactatgtt tttctccggg aagtgggcta
     4081 accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg gcactgatga
     4141 ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga cttcaggcct
     4201 tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt aatctagggc
     4261 tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag agtacggatt
     4321 tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg ggaattgctg
     4381 ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc attgagtacg
     4441 gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg agacttttgg
     4501 ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca ttcataaatt
     4561 caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca aacttatgta
     4621 caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt cacgttgaaa
     4681 tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct cttggggtag
     4741 tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa tagtattgtt
     4801 ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc
     4861 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt cttattccac
     4921 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga aatccccgga
     4981 agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta agttgtcaag
     5041 catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg
     5101 tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc tggttttcaa
     5161 attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg taatcgcact
     5221 cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg ttctccgtgg
     5281 agacattcca ttttcagcga gtgaattttc atctgttgct gtatccagtc atacagtgac
     5341 tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc tgaacatccc
     5401 cggacttctg cctcgatggc atacagttca ccgatacgca gcagggcttc cgttattctg
     5461 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     5521 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     5581 gccggaggca tttccgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5641 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5701 ttaccgggca tcagcacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     5761 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     5821 atttctgact ggcggtataa cggcagatag cctgcatatt ttccagcgac aacatgggcc
     5881 agaagccccg ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcacg
     5941 atatggtcac accggcaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg
     6001 ctgctgataa gctccagctg ctctgacaca tcacatccca gagaactgag ttcgccacca
     6061 caggccgggc agcattcctc ttccggacgg atgacccggg tttcacgggg aagtgaggca
     6121 ggtaacggtt tacgggctga agactggcgc aggggagatg gcagtaccgg gtcatattgc
     6181 tcacccagcg tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttccagt
     6241 atctgccgtt cggtttttgc gcgaagtttt tctgagcttt aaccgaactg catgcgctgc
     6301 agtttcgcaa ccagcgcctt cagccggttg atttcggaag cataagccgc cacccgctgt
     6361 gagagcaggc ggttgtattt cgccatctgg cggatggtgt cctgttgcgt ctgcaacagt
     6421 gcccgcaggc gggcattctc atgagcaagt gaggtgtcca tatcctcact ttacaacggg
     6481 ttatatgcgg atgccagcgc gttccgttcg tttcgggtgt ttccagtcga tgccctcaag
     6541 aagcatggat aactgggccg gagtaaggtg caccttgccg tcacgggtga ccggccagac
     6601 gaagcggccc cgctccaggc gtctggtaaa gaggcacagt ccgtcactgt cagcccacaa
     6661 cacttttatc tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc
     6721 atccttcagg acgttctgaa cttttgaggc caggccgtta aagccatttc gcatatcggt
     6781 gatacctgca accagccaga tacgcgaacc tgcagggaaa gatatcatca gtggctgctc
     6841 ccttttattt cgcggataag tatctgtaat aacgccggcg tcagtttacc tttaagcctg
     6901 agagttccgg ccggcagaac cagctcacag cacagactgt cggacggtgt atttatctgc
     6961 tctggttcct gtgccggagc cgggatttta ttatccggct ccggcgttaa cgtcacggga
     7021 agcagtgcag gcatattttt tccggaaggc agcaggccac ctttccggta ttgatggcgc
     7081 cagttgaaga gcaggttatc gttgattccg ttttcacggg cgatctgcgc cacacaggct
     7141 ccgggctgca gtgactgctc cactaaggcg attttaaatt cataaggaaa attaggccgc
     7201 cgg
//
LOCUS       DAJTYD010000183         7122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTYD010000183 DAJTYD010000000
VERSION     DAJTYD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.183"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..649
                     /locus_tag="OO585_003471"
     CDS             <1..649
                     /locus_tag="OO585_003471"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6630933.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            740..1987
                     /gene="tnaB"
                     /locus_tag="OO585_003472"
     CDS             740..1987
                     /gene="tnaB"
                     /locus_tag="OO585_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6630934.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2119..3294
                     /gene="mdtL"
                     /locus_tag="OO585_003473"
     CDS             2119..3294
                     /gene="mdtL"
                     /locus_tag="OO585_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6630935.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3269..4228
                     /gene="yidZ"
                     /locus_tag="OO585_003474"
     CDS             3269..4228
                     /gene="yidZ"
                     /locus_tag="OO585_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6630936.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4385..5134
                     /locus_tag="OO585_003475"
     CDS             4385..5134
                     /locus_tag="OO585_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6630937.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5156..5722
                     /gene="yieF"
                     /locus_tag="OO585_003476"
                     /gene_synonym="chrR"
     CDS             5156..5722
                     /gene="yieF"
                     /locus_tag="OO585_003476"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6630938.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5776..7113)
                     /gene="adeP"
                     /locus_tag="OO585_003477"
     CDS             complement(5776..7113)
                     /gene="adeP"
                     /locus_tag="OO585_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6630939.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1692 a         1823 c         1803 g         1804 t
ORIGIN      
        1 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
       61 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
      121 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
      181 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
      241 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
      301 cggtcacgcg gcattcgttg atgccggtaa actgctgccg aatatcccgg cagaccagtt
      361 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
      421 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
      481 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
      541 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
      601 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
      661 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
      721 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
      781 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
      841 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
      901 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
      961 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     1021 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     1081 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     1141 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     1201 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     1261 cgattactcc atcctgcgcg atgccaccag caccactgcg ggaacgtctt acttcccgta
     1321 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     1381 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     1441 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     1501 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     1561 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     1621 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     1681 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     1741 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     1801 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     1861 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     1921 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     1981 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     2041 atgtccaaat ggcaactcgc ctgatcctcc ttcaccacgt atgctttgcg tcaccttact
     2101 atcaggacgc tttagcccat gtcccgcttt ttgatttgta gttttgccct ggttttactt
     2161 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     2221 gccagcgaag cgcagttgca tattgcgttc tccgtatatc tggcggggat ggcagctgcg
     2281 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agccggtcgc catacccggc
     2341 gcggcgctat ttattattgc ctcggtgttc tgttcactgg ctgaaaccag cacgttattt
     2401 cttgcaggcc gatttctaca ggggttgggc gcaggctgtt gttacgtagt ggcgttcgcc
     2461 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcatt actcaacggt
     2521 attacctgca tcattccggt gttagcgcca gtgctcggac atctgattat gcttaaattc
     2581 ccgtggcaga gtctgttctg ggcgatggca atgatgggca tcgcggtact gatgttgtct
     2641 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     2701 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     2761 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     2821 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     2881 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     2941 caggtgttat tccttgcagc ggggatcacc cttgccgttt caccttccca tgcggtttct
     3001 ctgtttggta tcacactgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     3061 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     3121 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     3181 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttgct gattatgttc
     3241 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     3301 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     3361 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     3421 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     3481 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     3541 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     3601 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     3661 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     3721 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     3781 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     3841 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     3901 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     3961 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gctgcgcaac ccgacaatct
     4021 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     4081 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     4141 cctgctgtgg cataaacgga acagtcataa tccgaagatc gtctggttac gggaaaccat
     4201 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     4261 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     4321 gtccatcatt ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg
     4381 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     4441 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     4501 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     4561 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     4621 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     4681 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     4741 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     4801 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     4861 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     4921 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctggtgtggt ccgtggcggt
     4981 cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag
     5041 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     5101 gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     5161 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     5221 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     5281 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     5341 ggttgaagct ctggcggaac agatccgtca ggctgacggt gtggtgatcg tcacgccgga
     5401 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     5461 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     5521 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     5581 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatcggc aaaccggaga
     5641 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     5701 tattcagcga gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt
     5761 aatgcgctat cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc
     5821 aacgatgatt acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca
     5881 gtaggagata aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac
     5941 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact
     6001 tgaggtcatc aacacgccaa cgtagatcaa cgcgccagct gcagcgtagc ctggcaccat
     6061 tcccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     6121 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     6181 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     6241 catgcgcggg aatttcccct tctcatccgc cagacctgct ttatcggtca cgccaatcag
     6301 cgtaccggag gagtcaaaca agttgaccaa catgaaagag aaaatcaccc ctgccagccc
     6361 gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc
     6421 agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac
     6481 gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa
     6541 gccgaggata cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc
     6601 cgagttagcg acaatcacac ctgcgttttt cagccccatc atgccgataa acaggccgat
     6661 accgctggta atacccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac
     6721 gcggaaaatg gtcaaaagaa gcagtccgac agcgccccag aagattgcgc ccataccgat
     6781 ctgccacggc agccccattg cctggacaac cacaaaagcg aaaaaggcgt taagccccat
     6841 cgcaggagcc agcgccaccg gaaggtttgc aaacaatccc atcataatgc taccgaaagc
     6901 agcaatcaga caggttgtta cgaagacggc actggtgtcc atgccagcaa cgcccagaat
     6961 ttgtgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     7021 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     7081 gccagaagcc tgggttgtgt gttgttgact cattatctat tt
//
LOCUS       DAJTYD010000184         7074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTYD010000184 DAJTYD010000000
VERSION     DAJTYD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.184"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..151
                     /locus_tag="OO585_003478"
     CDS             <1..151
                     /locus_tag="OO585_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR6630940.1"
                     /translation="PDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFG
                     VKSER"
     gene            514..1551
                     /gene="dusA"
                     /locus_tag="OO585_003479"
     CDS             514..1551
                     /gene="dusA"
                     /locus_tag="OO585_003479"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6630941.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1685..1927
                     /gene="pspG"
                     /locus_tag="OO585_003480"
     CDS             1685..1927
                     /gene="pspG"
                     /locus_tag="OO585_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6630942.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2092..3075)
                     /locus_tag="OO585_003481"
     CDS             complement(2092..3075)
                     /locus_tag="OO585_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6630943.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3158..4573
                     /gene="dnaB"
                     /locus_tag="OO585_003482"
     CDS             3158..4573
                     /gene="dnaB"
                     /locus_tag="OO585_003482"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6630944.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4626..5705
                     /gene="alr"
                     /locus_tag="OO585_003483"
     CDS             4626..5705
                     /gene="alr"
                     /locus_tag="OO585_003483"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6630945.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5958..>7074
                     /gene="tyrB"
                     /locus_tag="OO585_003484"
     CDS             5958..>7074
                     /gene="tyrB"
                     /locus_tag="OO585_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6630946.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIA"
BASE COUNT         1682 a         1742 c         1870 g         1780 t
ORIGIN      
        1 tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa ttaattagct tcgcgaagtc
       61 gtttgaccca acctcaaagg aagagattaa aatacttaca gacacttcta aattaattta
      121 tgatttgttc ggggttaaat cggagagata atatgtgaag ttcttcgata gtatggaagg
      181 cattatataa aaggacccaa tatttattgg gtcctttttt ctctatcaat gctattagca
      241 gggagatata tcaccaaagt ttaatgtgtg attttttatt tatcgtcgaa cctggattgt
      301 ttatcattgg ccttaacaaa gttaacggct aataagatta tttccatcac ttcgtgagag
      361 cttcatgcct tgagaggatc tcaattttct tttgcaatga gacaggcgct tcctgttgtt
      421 atggtatagt accccgctat tgagcctcct gaatagtgat gctgaataac ataaccccat
      481 gatatatcga taaaataatc tctacatttg aaaatgcacg gtaattctga aatgcaaaaa
      541 atcaaccaaa ccagcgcaat gcctgaaaaa actgacgttc actggagtgg tcggtttagc
      601 gttgcaccaa tgctcgactg gacggacaga cattgccgct atttcttgcg tctgctttcc
      661 cgcaatacgt tgctgtatac cgaaatggtg accacagggg cgattattca cggtaaaggt
      721 gattacctgg cgtacagtga agaagaacat ccggtagcgt tgcaactcgg cggtagcgat
      781 ccggcggcgc tggcacagtg tgcgaagctg gcagaagcgc gtggatatga tgagatcaac
      841 ctgaatgtcg gctgcccgtc tgaccgggtg cagaacggca tgtttggtgc gtgtctgatg
      901 ggtaatgcgc agctggttgc cgattgcgtg aaagcgatgc gcgatgtggt gtcgattccg
      961 gtgacggtga aaacgcgtat tggcatcgat gaccaggaca gctatgaatt tctctgcgat
     1021 ttcatcaaca ccgtttccgg caaaggcgag tgtgggatgt tcatcatcca tgcacgtaaa
     1081 gcctggcttt cggggttaag cccgaaagaa aaccgtgaaa tcccgccgct cgattatccg
     1141 cgtgtgtatc aactgaagcg tgactttccg catctgacaa tgtcgattaa cggtggtatc
     1201 aagtcgctgg aagaggccaa agcacacctg caacatatgg atggcgtgat ggtcgggcgc
     1261 gaggcgtatc agaatccggg tattctggcg gcggtagacc gggagatctt tggttcctcg
     1321 gataccgatg cctatccggt ggcggtagtg cgcgccatgt atccgtacat tgagcgtgaa
     1381 ctcagccagg ggacgtatct cggccatatt acccggcata tgttgggctt gttccagggt
     1441 attcctggcg cgcggcagtg gcggcgttat ttaagtgaaa atgcccataa agcgggtgca
     1501 gacattaatg tgctggaaca cgcgctcaaa ctggtggcgg ataagcgtta acttttcacc
     1561 aaaaagtagt caaattcacc acgccctgcg caccgtcgcg gggcgttttg ctgttaaatc
     1621 aatagattat ttttggcatg attcttgtaa tgccagcaag agatttcata tttgggagag
     1681 catcatgctg gaactacttt ttgtgattgg cttttttgtc atgctgatgg tcaccggcgt
     1741 ttcgttgctg ggcattatcg ccgcgctggt tgtggcgacg gccattatgt tcctcggcgg
     1801 tatgctggca ttgatgatta agttgctgcc gtggttacta ctggcgattg cggtggtgtg
     1861 ggttattaag gcgattaaag caccaaaagt gcctaaatat cagcgttatg accgctggcg
     1921 ttactaaggg attgtgcgga tgatcacaac ctaaggtttt atccttagaa caaaatagga
     1981 attgataatc aaatctgtca ctattgcgcc tctaacagat tcatcgtgct gtaccctaca
     2041 tacagccgac tataaaaaga aagggcttcc caggtggaag ccctatttct tttatggaat
     2101 cagcaggctg gaaccttgcg tcgcccggct ttccagaatc tcatgcgcac gctgcgcatc
     2161 cttcagcgga tatttctgct gctcggcgac atcgacctta atcacaccgc tggcaatcaa
     2221 agagaacagt tcattactgg cctcggttaa ttcctcccgt gtggtgatat agccttgcag
     2281 ggaagggcgt gtcacataca acgagccttt ttgattgaga atgcctaagt tcacaccggt
     2341 aaccgcacct gatgagttgc caaaactgac cattaagccg cggcgttgca ggcaatccag
     2401 cgaccgttcc caggtgtctc tgcccacgga atcgtacact acgcgcactt tcttaccgcc
     2461 ggtgatctct ttcaatcgct cgaccagatt ctcttcacga tagttaataa cctgccacgc
     2521 gccggctttt agcgcgctct gcgctttttg cgcggttcct acggtgccga taagtttcgc
     2581 gcccagagct tttgcccact ggcaggcaat caagccaacg ccgccagccg ctgcgtggaa
     2641 caggaactgt tcatcgggtt taatttcata ggttttgcgc agcagataat aaaccgttaa
     2701 gcctttcagg aaagatgctg cagcttgctc aaaagaaata gccgcaggca gaatcgccgc
     2761 tttatccgca ttaatgttat gcacagagct gtaagcgcct aacgccgact gcgcatagac
     2821 tacacgatcg cctgccttaa tatgctttac accactgccg actttactca cgatgcctgc
     2881 cgcttcggta cctgatccgc tgggtagtga tggcggcggg taaaggccgc tgcggatata
     2941 cgtgtcgata aaattgatgc cgatggcttt attttcgacc tggatttcat tctctgccgg
     3001 atcggcagga gtgaactcta cggcttgaag tacttccggg ccaccgtgct tgtgaaattc
     3061 aattcgtgtt gccatgtgtc ctccagaacg tatcgtcagg gtctgcttca tatggtaaag
     3121 tttcgaccca ttctttatct cggtaactcc attcactatg gcaggaaata aacccttcaa
     3181 caaacagcag gctgaacccc gcgaacgcga tccacaagtt gccgggctga aagtgcctcc
     3241 gcactcgatc gaagcggagc agtcggtgtt gggcggttta atgctggata acgaacgctg
     3301 ggacgatgta gccgagcgtg tggtggcaga cgatttttac acccgcccac accgccatat
     3361 ctttactgaa atggcgcgtt tgcaggaaag cggtagtcct atcgatctga ttacccttgc
     3421 ggaatcgctg gaacgccagg ggcaactcga tagcgtcggt ggttttgctt atctggcaga
     3481 gctgtcaaaa aatacgccaa gtgcggcgaa catcagtgct tatgctgaca tcgtgcgtga
     3541 acgtgccgtt gtccgtgaga tgatctcggt tgcgaatgag attgctgaag ccggttttga
     3601 tccgcagggg cgtaccagcg aagatctgct ggaccttgct gaatcccgcg tctttaaaat
     3661 tgccgaaagt cgtgcaaaca aagacgaagg gccgaagaac atcgccgatg tgctcgacgc
     3721 aaccgtggcg cgtattgagc agttgtttca gcagccacac gatggcgtta ccggagtaaa
     3781 caccggttat gacgatctca acaaaaaaac cgctggcttg cagccgtcgg atttgatcat
     3841 cgtcgccgcg cgtccgtcga tgggtaaaac aacatttgcg atgaacctcg tcgaaaacgc
     3901 ggcgatgttg caggataaac cagtacttat cttctcgctg gagatgcctt cagaacagat
     3961 tatgatgcgt tctctggcgt cgctgtcgcg cgttgaccag actaaaatcc gtaccgggca
     4021 gctcgatgat gaagactggg cgcgcatttc cggcaccatg ggtattttgc tcgaaaaacg
     4081 caatatctat atcgatgact cctccggcct gacgccaacg gaagtgcgtt cccgcgcacg
     4141 acgtattgcc cgtgaacacg gcggcatcgg gcttatcatg atcgactacc tgcaactgat
     4201 gcgcgtaccg gcgctttccg ataaccgtac gctggaaatt gcagaaatct ctcgctcgct
     4261 gaaagcactg gcgaaagaac tgaacgtgcc ggtggtggcg ctgtcccagt tgaaccgttc
     4321 tctggaacaa cgtgccgaca aacgcccggt caactccgac ctgcgtgaat ctggctctat
     4381 cgagcaggat gcggacttga tcatgtttat ctatcgtgat gaggtgtatc acgaaaacag
     4441 tgatttaaaa ggcatcgcgg aaattattat cggtaaacaa cgtaacggcc caatcgggac
     4501 ggtacgcctg acctttaacg gtcaatggtc gcgcttcgac aactatgcgg ggccgcagta
     4561 cgacgacgaa taataattat tttatgaatt aggcaattaa agcaaacact tatcaaggaa
     4621 cacaaatgca agcggcaact gttgtgatta accgccgcgc tctgcgacac aacctgcaac
     4681 gtcttcgtga actggcccct gccagtaaaa tggttgcggt ggtgaaagcg aacgcttatg
     4741 gtcacggtct tcttgagacc gcgcgaacgc tccccgatgc tgacgccttt ggcgtagccc
     4801 gtctcgaaga agctctgcga ctgcgtgcgg ggggaatcac caaacctgta ctgttactcg
     4861 aaggcttttt tgatgccaga gatctgccga cgatttctgc gcaacatttt cataccgccg
     4921 tgcataacga agaacagctg gctgcgctgg aagaggctag cctggacgag ccggttaccg
     4981 tctggatgaa actcgatacc ggtatgcacc gtctgggcgt aaggccggaa caggctgggg
     5041 cgttttatca tcgcctgaca cagtgcaaaa acgttcgtca gccggtgaat atcgtcagcc
     5101 attttgcgcg cgcggatgaa ccaaaatgtg gcgcaaccga gaaacaactc gctatcttta
     5161 ataccttttg cgaaggcaaa cctggtcaac gttccattgc cgcgtcgggt ggcattctgc
     5221 tgtggccaca atcgcatttt gactgggtgc gcccgggcat cattctttat ggcgtctcgc
     5281 cgctggaaga tcgctccacc ggtgccgatt ttggctgtca gccagtgatg tcactaacct
     5341 ccagcctgat tgccgtgcgt gagcataaag ccggagagcc tgttggttat ggtggaacct
     5401 gggtaagcga acgtgatacc cgtcttggcg tagtcgcgat gggctatggc gatggttatc
     5461 cgcgcgccgc tccatccggt acgccagtgc tggtgaacgg tcgcgaagta ccgattgtcg
     5521 ggcgcgtggc gatggatatg atctgcgtag acttaggtcc acaggcgcag gacaaagccg
     5581 gggatccggt cattttatgg ggcgaaggtt tgcccgtaga acgtatcgct gaaatgacaa
     5641 aagtgagcgc ttacgaactt attacgcgcc tgacttcaag ggtcgcgatg aaatacgtgg
     5701 attaatcgtt ctgtaatatt tgattgtctg tgccggatgc ggcgtgaatg ccttatccgg
     5761 ccaataaaat cctaaaaatt caataagttg atgttctttc atgctcttat aaaggtcgtg
     5821 cctctggcgg atgttcgttt gtcatgagtc tcactctgtt gctaattgcc gttcgctcct
     5881 gaacatccac tcgatcttcg ccttcttccg gtttattgtg ttttaaccac ctgcccgtaa
     5941 acctggagaa ccatcgcgtg tttcaaaaag ttgacgccta cgctggcgac ccgattctta
     6001 cgcttatgga gcgttttaaa gaagaccctc gcagcgacaa agtgaattta agtatcggtc
     6061 tgtactacaa cgaagacgga attattccac aactgaaagc cgtggcggag gcggaagcgc
     6121 gcctgaatgc gcagccccat ggcgcttcgc tttatttacc gatggaaggg cttaacagct
     6181 atcgccatgc cattgcgccg ctgctgtttg gtgcggacca tccggtactg caacaacagc
     6241 gcgtagcaac cattcaaacc cttggcggct caggggcatt gaaagtgggc gcggatttcc
     6301 tgaaacgcta cttcccggaa tcaggcgtct gggtcagcga tcctacctgg gaaaaccacg
     6361 tagcaatatt cgccggggct ggattcgaag tgagtactta cccctggtat gacgaagcga
     6421 ctaacagcgt gcgctttaat gacctgttgg cgacgctgaa aacattacct gcccgcagta
     6481 ttgtgttgct gcatccatgt tgccacaatc caacaggtgc cgatctcact aatgatcagt
     6541 gggatgcggt gattgaaatt ctcaaagccc gcgagcttat cccattcctc gatattgcct
     6601 atcaaggatt tagtgccggt atggaagagg atgcctacgc tattcgcgcc attgccagcg
     6661 ctggattacc cgctctggtg agcaattcgt tctcgaaaat tttctccctt tacggcgagc
     6721 gcgtcggcgg actttctgtt ctgtgtgaag atgccgaagc cgctggccgc gtactggggc
     6781 aattgaaagc aacagttcgc cgcaactact ccagcccgcc gaatttgggt gcgcaggtgg
     6841 tggctgcagt gctgaatgac gaggcattga aagccagctg gctggcggaa gtagaagaga
     6901 tgcgtactcg cattctggca atgcgtcagg aattggtgaa ggtattaagc acagagatgc
     6961 cagaacgcaa tttcgattat ctgcttaatc agcgcggcat gttcagttat accggtttaa
     7021 gtgccgctca ggttgaccga ctacgtgaag aatttggtgt ctatctcatc gcca
//
LOCUS       DAJTYD010000185         6931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTYD010000185 DAJTYD010000000
VERSION     DAJTYD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.185"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..249
                     /locus_tag="OO585_003485"
     CDS             <1..249
                     /locus_tag="OO585_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6630947.1"
                     /translation="PAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGLAALDST
                     ATPCYDRDTAQALVNVGAQIAAMTLGELASWLAENLQS"
     gene            246..2246
                     /locus_tag="OO585_003486"
     CDS             246..2246
                     /locus_tag="OO585_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6630948.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2371..2832
                     /locus_tag="OO585_003487"
     CDS             2371..2832
                     /locus_tag="OO585_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6630949.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2873..3343)
                     /locus_tag="OO585_003488"
     CDS             complement(2873..3343)
                     /locus_tag="OO585_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6630950.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3390..4109)
                     /gene="yehT"
                     /locus_tag="OO585_003489"
     CDS             complement(3390..4109)
                     /gene="yehT"
                     /locus_tag="OO585_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6630951.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(4106..5791)
                     /gene="btsS"
                     /locus_tag="OO585_003490"
     CDS             complement(4106..5791)
                     /gene="btsS"
                     /locus_tag="OO585_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6630952.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            6013..6078
                     /locus_tag="OO585_003491"
                     /pseudo
     CDS             6013..6078
                     /locus_tag="OO585_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            6306..6554
                     /locus_tag="OO585_003492"
     CDS             6306..6554
                     /locus_tag="OO585_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6630953.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(6670..>6931)
                     /locus_tag="OO585_003493"
     CDS             complement(6670..>6931)
                     /locus_tag="OO585_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6630954.1"
                     /translation="QHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQDAVD
                     LEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
BASE COUNT         1760 a         1778 c         1694 g         1699 t
ORIGIN      
        1 ccagcgaaaa gcgtcattat cctcgtgagc gatttttacg aagggggttc atcatcatta
       61 ctgacgcatc aggtgaaaaa gtgtgtccag agcggcatca aagtgctggg actggcagcg
      121 ctcgatagca cagcaacacc ttgctatgac cgcgatacgg cccaggcgct ggttaatgtc
      181 ggcgcacaaa tagccgccat gacactgggc gagctggcat catggcttgc ggagaatctt
      241 cagtcatgaa ttcactacgt ccggaattat tagaactgac accgcaggcc ctgacggcgt
      301 taagcaatgc cggttttgtt aagcgcagtc ttaaggaact ggaaaatggc aacgtcccgg
      361 agatcagcca tgagaacggc gctttaatcg ccaccttcag tgacggtgtc cgtacccagc
      421 tggcgaacgg ccaggcactg aaagaggctc agtgcagctg cggggccagc ggtatgtgcc
      481 gtcatcgcgt gatgctggtg ttaagttatc aacgactttg tgctaccgcc cagcctacgg
      541 gaaaagaaga ggaatgggat ccggcaatct ggctggaaga actggctacc cttcccgatg
      601 ccacccgcaa acgcgcacag gcgctggtcg ctaaaggcat caccattgag ttgttctgtg
      661 cgccgggtga aattccctct gcccgcttac cgatgagcga tgtgcgtttt tattcccgca
      721 gcagtattcg tttcgcccgt tgtgattgta ttgaaggcac actttgcgaa catgtcgtac
      781 tggcagtaca ggcctttgtc caggcaaaag cgcagcaagc ggaattgact catttaatct
      841 ggcagatgcg cagcgaacac gtcacatcat ctgacgatcc gtttgccagc gaagaaggca
      901 aaacgtgtcg tcaatatgtt caacaactaa gccaggcatt atggctgagc ggcatcagcc
      961 agccgcttat tcactacgag gccgctttca gccgcgcgca gcaagcagcg gaacgctgca
     1021 gctggcgatg ggtgagtgaa tcgctacggc aactgcgcgc cagcgttgat gccttccacg
     1081 cccgcgccag ccactatcat gccggagaat gcttacgtca gcttgcggca ttaaacagtc
     1141 gattaaactg cgcacaagag atggcccggc gcgacagtgt tggtgaagtt cctcctgtgc
     1201 cgtggcgcac ggtcgttggc tctggcattg ccggagaagc aaagcttgat catctgcggc
     1261 tggtgtcttt aggtatgcgt tgctggcagg atattgagca ttatggttta cgcatctggt
     1321 ttaccgatcc cgacaccggc agtattttgc atctttcgcg tagttggccg cgaagtgaac
     1381 aggaaaactc accggcagct acgcgtcggc tgtttagttt tcaggctggc gcactggcgg
     1441 gtgggcaaat tgtttcacaa gcagcaaaac gcagtgccga tggcgagctg ctgttagcta
     1501 cccgcaaccg cttaagcagc gttgtgccgc tgtcgccaga cgcctggcaa atgttgagcg
     1561 cgccgttacg ccagccgggc attgtggctt tgcgggaata tttacgccag cgtccccccg
     1621 cctgcatacg gcctcttaat caggtggata acttatttat tctgccggtc gctgagtgta
     1681 tttcactcgg ttgggacagc ggccgccaga cgctggatgc gcaggtaatc agcggcgaag
     1741 gtgaagataa tctgctgacg ttatcccttc cggcatctgc ctgttctcct tttgccgttg
     1801 aacgcatggc ggcgcttttg caacaaacag acgaccccgt gagtctggtt tctggctttg
     1861 tcagctttgt agaggggcaa ttgacactgg aaccacgggt gatgatgaca aaaacccgtg
     1921 cctgggcgct ggacgcggaa actgcgcctg tggcaccgct accttctgcc agcgttttgc
     1981 ctgtgccgtc taccgctcat cagttgctga tgcgctgcca ggcgttactt attcaactgc
     2041 tccataacgg ctggcgctat caggaacaga gcgctattag tcaggcagag ttgctggcga
     2101 atgacctcac cgcggtgggt ttttatcggc tggcacatgt gttggtacaa tttcgtaata
     2161 cagaaagcga ggcacgggta gaagcaatga ataacggtgt tttgctttgc gaacaattat
     2221 tccccatgct tcagcaacaa ggatgaaata gtgcttttta ctaagagttc tactccagtt
     2281 ccggactgct cacgccacgg tattaggcat atcctatata gcccctggtg agagtcacca
     2341 gtttcttgat taaataaaat ggagttttac atgaaggctt tcaataagct gttttccctc
     2401 gttgttgcat ctgttctggt tttctctctt gctggctgcg gtgacaaaga agaatcgaag
     2461 aaattcagcg ccaatctgaa cggcactgaa attgccatta cctatgtcta caaaggtgac
     2521 aaggtgctta agcaatcttc tgaaaccaaa attcaatttg cctccattag tgcaaccacc
     2581 aaagaagatg ctgccaagac acttgagccg ttaagcgcca aatacaaaaa catcgcgggt
     2641 gttgaagaaa aattaaccta taccgatacc tacgcgcagg aaaacgtgac tatcgatatg
     2701 gaaaaagtgg attttaaagc cctgcagggt atttcaggaa tcaacgtttc tgctgaagat
     2761 gccaaaaaag gtatcactat ggcgcaaatg gaactggtga tgaaagccgc tggttttaaa
     2821 gaagtgaaat aatctgtcgg cggccatgtt tcgtatggcc gccatacccg tcttagcttt
     2881 tcttcacatg ctggcgcgct gccagtccac gcagaaaata acgtaaaaat tgatcgccgc
     2941 attcgcggaa gtttttatga tccggtgcgc gcatcatcgc tgtaatttcc ggcatcgaaa
     3001 cgcggaactg ctgttcggtg aggatagcca gaatgtcatc ggttttcagc gaaaacgcga
     3061 tgcgtaattt tttcagcacg atgttgttat taatgcgacg ttccggctcc agtgccggag
     3121 cagactcatc cttgccgcgt ttttcataaa tcaggccatt gaggaatgac gacaaaacaa
     3181 tgtccggaca acgctgaaaa ccctcttcgt cttctttacg tagccagacg gcgatctgtt
     3241 ccgcggtggc ttcgacatta cccagcgcca gaatacgcac caggtcatta ttattggctt
     3301 tcaaaatgta gcgcacgctg cgcagaatat cgttacttag catgaggcct tcaggtgttg
     3361 atgaggcaaa aagccatttt agcagtcttt tacaggccaa tcgcctcttt taagcttttc
     3421 agataacggc ggctgaccgg cacggttaag ccattacgca aaatcaactc ggcctggccg
     3481 ttatcttcca gacgaatctc ctgtaaatgc gcgaggttaa ccagatactg acgatggcag
     3541 cgcagtagtg gtgtacgact ttccagggta cgtaatgtca attcggtaaa gccctctttc
     3601 ccttcgtggc tggtaacgta gacaccgctc atccgactgc tgacaaatgc cacatctttc
     3661 atttgcagca aataaatccg actatgcccc gtacaaggga taaatttcag cgcctgttga
     3721 ttttccggta acagcgaaac atcctgcttg ctgcgctcct gacgcaatcg cgccagcgtt
     3781 ttctccagtc gcgcttcatc aattggcttc agcagataat caaaggcatg ttcttcaaag
     3841 gctttaatag cgtattcgtc aaacgcagtg agaaaaacaa tatacgggcg atgttccggg
     3901 tcaagcatcc ccaccatttc cagaccactg atgcgcggca tctggatatc gagaaacagc
     3961 acatccgggc gcagtttatg caccgcgccg atcccttcca cggcgtttga acactctcca
     4021 acgatttcaa tatcgctctg ctcctgcaaa aatacacgca ggttctcccg tgctaacggt
     4081 tcatcatcga caattaagac tttaatcatg cctcgtccct ccatggtagt tgtaacgtta
     4141 ttcgggtgta actatcaggc tcacaggcga cgcttattcc atagtcatcg ccaaaccgtt
     4201 cacgtaaacg cttatccacc agattcatcc ccagcccact ggcattggtt accggttgat
     4261 acaaaccggc attgtcttcg atctccagca tcaaatgttg cccctcacgt cgggcgctga
     4321 ttgccactcg ccctgtatcc agcagttgtg atgtcccatg tttaatggcg ttttccacta
     4381 tcggttgcag ggtaaacgcg ggcaattgct gctgggataa ttcttgcgga atagcaatgt
     4441 tgacctgcaa ccgcgactgg aagcgcgcct tttcaatttg cagataagcg ttcacatgtt
     4501 caatttcgtc ggcgagagta acaaactccg aaggccgctt taagtttttg cggaaaaaag
     4561 tggaaagata ctgcaccagc tggctggcct gttcgctgtc gcggcggatc accgctttaa
     4621 tggtgttaag cgcattaaac aaaaaatgag gattcacctg ggcgtgaagc agtttgattt
     4681 ctgactgggt gagcatcgct ttttgccgct catattgccc ggcaagaatc tgcgccgaaa
     4741 gcagttgcgc aatcccctcg cccagcgtgc ggtttattga actgaataaa cggtttttgg
     4801 cttcatacaa tttgatggtg cccatcaccc gctgattttc accacgcaac ggaattacca
     4861 gcgtcgaccc cagtttgcat tgcggatgca aagagcaacg ataaggtact tcgttgccat
     4921 cagcgtagac cacttcaccg gtttcaatcg cttttaaggt gtaagtcgaa gaaatcggtt
     4981 tgccgggtaa atggtggtcg tcaccaattc cagtaaaggc cagcaatttc tctcgatcgg
     5041 taatcgcgac tgcaccaata tccagctcct gatacagcac ctgtgccact ttcatgctgt
     5101 tcacttcgtt aaacccctgg cgcaaaatgc cttccgtcga ggcagccact ttcagcgcag
     5161 tggcagaaaa agccgaagtg tatttttcaa acatcgcgcg tttatcgagc aatatacgca
     5221 taaacagcgc cgcgccgacg gtattggtga ccatcattgg tgcagcaata ttactcacca
     5281 gacgcaccgc atcttcataa ggtcgggcga tcgccaggat gatcaacatt tgcaccattt
     5341 cagcgacgaa cgtgacggca ccggcggtaa tggggttaaa gactttatca gtacgcccgc
     5401 gacgaatcag gatgctgtgt accaggccgc cgagcaatcc ttcaacgatg gtcgagatca
     5461 tgcaacttag cgcggtcatg ccccccatcg aatatcgatg taacccgccg gtcaggccaa
     5521 ccagcccacc gacgaccgga ccgccgagta agccgcccat taccgcgcct atcgcacggg
     5581 tattggcaat agaatcgtca atgtgcaacc caaaccaggt gcccatgatg cagaagatgg
     5641 aaaagacgat gtagcagaga aatttatgcg gcagacgaac cgtgacctgc attaacggta
     5701 tgaataatgg cgttttactc attaaccacg caatgactaa aaaaacgcac atctgctgaa
     5761 gcagcagcaa caccagatta aaatcgtaca tacccgcaaa ccacacttcc ctttaaaacg
     5821 cgtaacatac attgcctgcg tttaactttc tttgaactct tgcagaaaaa tgagaattcg
     5881 tgagtacgat cactcaaaat cgcctggcaa aaataaaatc accctataga tgcacaaaaa
     5941 acgggcaaaa ctacctggtt cgcaaaactg cgtctaaagt taaaccggga cctcgcgagc
     6001 aagggtgaga cgatggcgct ttacacaatt ggtgaagtgg cgttgctttg tgatattaac
     6061 cctgtcacgt tacgcgcgtg acgcaacatt ttaaaaaatc gacgaagatc gtccaggagc
     6121 gccgtttttc aagggttgga tagatgattc gtttaccatg tcgacgaact tcgacgaatt
     6181 tcgccgataa aaagtgtccc atacatgccc cattgatgca ccataaacaa tatcagaaat
     6241 tgatactggt tatccataca gataaaaata acacctgtac ataactacag ttacaggagg
     6301 gtgttatgcg tgttgagatc tgtatcgcta aagaaaaaat cactaaaatg ccaaacggtg
     6361 ctgtggatgc gttaaaggaa gaattaaccc gacgcatcag taaacgttat gacgatgtag
     6421 aggtgatcgt aaaagccacc agcaacgatg gcctttctgt tacacgcacc gcagataagg
     6481 attctgcaaa aacatttgtt caggagactc tgaaagatac ctgggagtct gctgacgagt
     6541 ggtttgttca ctaataaaca cgtaaaatcg gtaacggctg gaaatcattc aatactcgca
     6601 ctatcgaaag tttaccagcc aaccgcagca cgtcctgcat acgtcgtgtc tacggttttt
     6661 ctttttcgct tacattgtgt ctggttcttc cggccactca atatcaggtg cagttgatgt
     6721 atcaacacag ttcagcaaca cccgatactt cttccaggct tccagcaacg agttttcttc
     6781 ctccgttgcg atctccagat ctacagcatc ctgaagtggc gcaatatgct cactgaattc
     6841 ctggatgtag aactgtgtgg tgacggtctt ccagccattc ggctcctgct gtatcgaagc
     6901 ataccaggct atttcaatat cgctatgctg c
//
LOCUS       DAJTYD010000186         6823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTYD010000186 DAJTYD010000000
VERSION     DAJTYD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.186"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(26..1162)
                     /locus_tag="OO585_003494"
     CDS             complement(26..1162)
                     /locus_tag="OO585_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6630955.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1165..1527)
                     /locus_tag="OO585_003495"
     CDS             complement(1165..1527)
                     /locus_tag="OO585_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6630956.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2064..3977
                     /gene="mtlA"
                     /locus_tag="OO585_003496"
     CDS             2064..3977
                     /gene="mtlA"
                     /locus_tag="OO585_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6630957.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4207..5355
                     /gene="mtlD"
                     /locus_tag="OO585_003497"
     CDS             4207..5355
                     /gene="mtlD"
                     /locus_tag="OO585_003497"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6630958.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5355..5942
                     /gene="mtlR"
                     /locus_tag="OO585_003498"
     CDS             5355..5942
                     /gene="mtlR"
                     /locus_tag="OO585_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6630959.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(5954..6163)
                     /locus_tag="OO585_003499"
     CDS             complement(5954..6163)
                     /locus_tag="OO585_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630960.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6448..6810
                     /locus_tag="OO585_003500"
     CDS             6448..6810
                     /locus_tag="OO585_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6630961.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1698 a         1728 c         1770 g         1627 t
ORIGIN      
        1 agttccataa tctcccccct ccccctcaat gatccaaata aagataatgc atccagctgg
       61 tcattcttag cagcactttc cgcatcaccg aaacatggct gaaatggtcg gagtaaaccg
      121 ccacctgggc gtagatgccg tcgggtaagg catcgatatc atcgttaggg tccagttcaa
      181 tggttccctg cacaccgtcc gtgccgggca cgaccgttaa tgattgcaat cccccctgcg
      241 cctgataaga accgcctggc acgacaggta aaatactagt cagtttgccg tgaaacacct
      301 gcccaggtag cgcgttaaac accacttccg catcatcgcc gggtttcaga cgtaacagcg
      361 agttttgccg aaattgggcg acaatttgcc gtttttgctc ggggatgaag accatcaccg
      421 gacgcagcgg caaggcagct gcgtatgtac ctgggcggat cagtacctga gtaacgtagc
      481 cattgctcgg cgcgcgaatg acagtctgct caaggttata ttttgcttca gtaagttgcg
      541 ctcttaagct cacaatctgc gattgctcgc cgttaaccat actgtcgagc tggctctgga
      601 tctgcgcctg ctccgccacc gagcctttca ccagcgcatc ctgcgcgagg aaattttgcc
      661 gcgcatcgtc gatgtcacgt tccgagaacg gattcaccgc cgcctggctg cctttcaagt
      721 aacgttgata atttttaaac agacggtcgc gctccgctga aacctgggtg gtgttggcct
      781 gcgcttcagt gagctgcgca cgcagcgtct ttatattatg cgtcgccgtc atcaggtcag
      841 cctgaagtct gtcaactcgc gcctggtaac gaaccgggtc gagcttaaaa agcacctcgc
      901 ccttttgaat aagctgatta ttcttgtcag tgacttcagt aacaattccc gtcacctgtg
      961 gcgtgatagg gatcgctatc actgcctttt gcgcggtaaa agtgtaaggg tggttgtagt
     1021 tcatcaacaa aatcaaacca ctcaccagaa acacgcctcc cagcgccgcc gtcgccagcg
     1081 tccactgatt taccggaatg cggaagattt taaagaccgc ccacgccagc gccacgtaag
     1141 ttaaaacaat caatagatcc ataattagat ctccgggaac gtggaatagt cagtcttttt
     1201 ctcggcggcg agttgatgct ccagccggga aattcggtca gaaagtgagg cgatttccgg
     1261 gtcagtcgct ttctcctgcg acgcaacatg tgactgcata ccccagccac gctccggttg
     1321 atagagcgtc gcccagatcc acagaaacgg ccagataaca tgcagagtaa acaggctcac
     1381 ccagcccgcc gtatgaatag cgtcggcatg ggggtgattg cgctttttgg caatcagata
     1441 agggatgtca tgtatcgcga tgattccata aaaaatcacc agaaagacga agatcagcac
     1501 tcccaacgcg aaatagttta gaaacatatc tgcctcggat tcacgtttat cagtgttgtt
     1561 tttgggctgg cagccagaag ggagtcaggc tgatattttg acaataatcc gggttcgcga
     1621 ttctcgccat aacaccaaag aataattttt agaggtgatg agttgcttag ttacataacg
     1681 attgtatgac gaaggcataa catgctgtag atcacatcag gtgaacgccg taagaaaata
     1741 tcttgtgatt cagatcacaa agattcaaca aaccatcaaa acaaaaatgt gacactactc
     1801 acatttaaat gccattttta gcgaaaatcg ccgccttgtt gcttttttac acaagcgttt
     1861 tgtgatgaac gtcacgtcaa ttacctctct acctcctata tttatgtgat taatatcaca
     1921 caaaaggccg tcgactggac agttaaccga ttcagtgcca gatttcgcag tatctacaag
     1981 gtccggctac ctctgccgcc acattaacaa aaaacctcgg gcttccagcc tgcgcgacag
     2041 caaacataag aaggggtgtt tttatgtcat ccgatattaa gatcaaagtg caaagctttg
     2101 gtcgtttcct cagcaacatg gtgatgccaa atatcggcgc gtttatcgcg tggggtatca
     2161 tcaccgcgtt atttattcca acagggtggt taacgaacga gacgctggcg aagctggtcg
     2221 ggccgatgat cacttatctc ctgccgctgc tgatcggtta taccggtggt aagctggtag
     2281 gcggcgaacg tggcggcgta gtcggtgcca tcaccaccat gggcgttatc gtcggcgcag
     2341 acatgccgat gttcctcggt tctatgattg caggtccgct gggcggctgg tgcattaagc
     2401 acttcgaccg ctgggtagac ggtaagatca aatccggttt tgagatgctg gtgaataact
     2461 tctccgcagg catcatcggg atgatcctcg ctattctggc attcctcggc attggcccga
     2521 ttgttgaagc cctgtccaaa atgctggctg cgggcgttaa cttcatggtt gtccatgaca
     2581 tgctgccgct ggcgtctatc tttgttgagc cggcgaaaat cctgttcctc aacaacgcca
     2641 ttaaccacgg tatcttctcg ccgctgggta ttcagcagtc ccatgaactg ggtaaatcaa
     2701 tcttcttcct gattgaagct aacccaggtc caggtatggg cgtgctgctg gcgtacatgt
     2761 tctttggtcg tggtagcgct aaacagtctg cgggcggtgc ggcaatcatc cacttcctgg
     2821 gtggtatcca cgaaatctac ttcccgtatg tgctgatgaa tccgcgtctg atcctcgcag
     2881 tcatcctcgg cggtatgact ggcgtgttca cgctgactat cctgggcggt ggtctggttt
     2941 ctccggcatc tccgggttct atccttgctg tactggcgat gacaccaaaa ggggcttatt
     3001 tcgctaacat cgcgggtgtg tgtgcggcga tggctgtctc cttcgttgtc tctgctattt
     3061 tgctgaaaac cagcaaagtg aaagaagaag atgatattga agcagcaact cgtcgtatgc
     3121 aggacatgaa agctgagtct aaaggcacat ctccgctgtc tgcgggcgat gtgactaacg
     3181 acctgagcca cgtacgtaaa atcatcgttg cctgtgatgc cggtatgggt tcaagtgcga
     3241 tgggcgcagg cgtgctgcgt aagaaaattc aggatgcagg tctgtcgcag atttcggtca
     3301 ctaacagcgc gatcaacaac ctgccgccag atgtggacct cgtcatcact caccgtgacc
     3361 tgaccgaacg cgctatgcgc caggttccgc aggcacagca tatttcgctg accaacttcc
     3421 tcgacagcgg cctgtacacc agcctaaccg aacgtctggt tgctgcccag cgccatactg
     3481 aaaacgaagt gaaagtgaaa gacagtctga aagacagctt tgacgattcc agtgctaacc
     3541 tgttcaaact gggcgcggag aacatcttcc tcggtcgcaa agcagcaacc aaagaagaag
     3601 cgattcgttt tgctggcgag cagctggtga aaggcggtta cgttgagccg gaatacgttc
     3661 aggcgatgct ggatcgtgaa aaactgaccc cgacttatct gggtgagtct atcgcggtgc
     3721 cacacggtac ggttgaagcg aaagatcgcg tactgaaaac gggcgtggtg ttctgccagt
     3781 acccggaagg cgtgcgcttc ggtgaagagg aagatgacat tgcccgtctg gtgattggta
     3841 ttgcagcccg taacaacgag cacattcagg ttatcaccag cctgaccaat gcactggatg
     3901 atgagtccgt catcgagcgt ctggcacaca ccaccagcgt ggatgaagtg ctggaactgt
     3961 tggcaggtcg taagtaatcc aatcccaccc tctccacatg gagaaggtgg ggttaattgc
     4021 ctgatgcgct acgcttatca ggcctacagg atgcatcacg atttgttgaa tttgcacgtt
     4081 cttgtaggcc ggataaggcg cttacgccgc atccggcgct gcccctctcc tcacggagag
     4141 ggtttgggtg agggaaaagc ctcaccccag ccctctcggg taaaaacatt gatgaaggtt
     4201 aatactatga aagcattaca ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa
     4261 ctgctggcag acgcgggtat ccaactgacg tttgccgatg tcaatcaggt ggtacttgat
     4321 gccctgaatg cccgtcatag ctatcaggtt catgtggttg gtgaaaccga gcaggtagat
     4381 accgtttccg gcgtcaatgc tgtcagcagc attggtgatg atgtcgttga tctgattgct
     4441 caggtcgatt tagtcactac cgccgttggc ccggttgtgc tggaacgtat tgctccggct
     4501 atcgccaaag ggctggtgaa acgcaaagaa caaggtaatg aatccccgct gaacatcatc
     4561 gcctgtgaaa acatggtacg cggtaccacg cagctgaaag gccatgtgat gaacgccctg
     4621 ccggaagacg ccaaagcgtg ggtagaagaa cacgttggct ttgtcgattc cgccgttgac
     4681 cgcatcgtac cgccttcggc ttcggcaact aacgatccgc tggaagtgac ggtagaaact
     4741 ttcagcgaat ggattgtcga taaaacgcag ttcaaaggcg cactgccgaa catcccaagc
     4801 atggagttaa ccgacaacct gatggcattt gtcgaacgta aactcttcac cctgaacacg
     4861 ggtcatgcta taaccgcgta cctcggaaaa ctggccggtc atcagaccat tcgtgacgct
     4921 attctcgacg agaaaatccg cgcggtggta aaaggtgcga tggaagaaag tggtgcggta
     4981 ctgatcaagc gctacggctt tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc
     5041 ggtcgttttg agaacccgta tctgaaagat gatgtagagc gcgtaggccg tcagccgctg
     5101 cgtaaactga gtgctggcga ccgtctgatc aagccactgc tcggtacgct ggaatacagc
     5161 cttccgcaca agaatctgat tcaggggatt gctggtgcaa tgcacttccg cagtgaagat
     5221 gatccgcagg ctcaggaact ggcagcactg atcgctgaca aaggtccgca ggcggcgctg
     5281 gcacagattt ccggtcttga tgccaacagt gaggttgtat ccgaggcggt aaccgcttat
     5341 aaagcaatgc aataatggtg gaccaggcgc aggacaccct gcgcccgaat aacagattgt
     5401 cagatatgca ggcaacaatg gaacaaaccc aggcctttga aaaccgtgtg cttgagcgtc
     5461 tgaatgctgg caaaaccgtg cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg
     5521 cggtaaatct tctggtgctt caggtattcc gcaaagacga ttacgcggtg aagtatgctg
     5581 tagaaccgtt actcgacggc gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca
     5641 tttacgggtt gggcgtcatt aaccgccagg aatacgaaga tgcggaactg ctgatggcat
     5701 tgcgtgaaga gctaaatcac gacggcaacg agtacgcctt taccgacgac gaaatccttg
     5761 gaccctttgg tgaactgcac tgcgtggcgg cgttaccacc gccgccacag tttgaaccag
     5821 cagactccag tttgtatgca atgcaaattc agcgctatca acaggctgtg cgatcaacaa
     5881 tggtcctttc actgactgag ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt
     5941 aagcaacgtc tgcttactgc ccctctacct gcttcggccg ataaagccga cgataatact
     6001 ccagacgttg tagatataac ggcacgcttt catcgggtat tccggacgga atcgcgttac
     6061 ggggaggaag ttttttcaga tactcccgga acgcctggct tgatgccatg aaatctacgg
     6121 ctttatcgat aagaagcgga acgttttcac ctaatttgcc catgatgata tctccgtatt
     6181 accccgcgcc gggaatgcgc ggccaccaat tttagtttag gttcccggta aagatgacat
     6241 taaggaaaac gtgctgaatc ctcaataatc atccgctgct gtttattatc ttttttctat
     6301 agatttctta attaatcaac gaattgcgta gtttttgagc atgttaatga ttacgttgca
     6361 gaactattac aatgtcatca gttgtgaaca aagcacctgg tcgcgcatac taggggctat
     6421 aaatttatct ttatcagaag ccatcacatg aaagaagtcg aaaaaaacga aatcaaacgt
     6481 ctcagcgatc gcctggacgc catccgccac cagcaggccg atctgtcgct ggttgaagcc
     6541 gcagacaaat atgccgagct ggaaaaagag aaagccacgc tggaagcaga aattgctcgc
     6601 ctgcgtgagg ttcatagcca aaaactgagt aaagaagcac agaagctgat gaagatgccg
     6661 tttcagcgcg caattaccaa aaaagagcag gctgatatgg gcaagctgaa gaaaagtgtt
     6721 cgcggactgg tggttgtgca cccaatgacc gcgctgggcc gtgaaatggg cctgcaggag
     6781 atgactggat tctctaagac tgcgttttaa gcgcctcaga cga
//
LOCUS       DAJTYD010000187         6779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTYD010000187 DAJTYD010000000
VERSION     DAJTYD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.189"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO585_003501"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO585_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6630962.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO585_003502"
     CDS             2089..2238
                     /locus_tag="OO585_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630963.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2994..3434)
                     /locus_tag="OO585_003503"
     CDS             complement(2994..3434)
                     /locus_tag="OO585_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6630964.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3652..4041
                     /locus_tag="OO585_003504"
     CDS             3652..4041
                     /locus_tag="OO585_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6630965.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4085..4489
                     /locus_tag="OO585_003505"
                     /pseudo
     CDS             4085..4489
                     /locus_tag="OO585_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4596..5432)
                     /locus_tag="OO585_003506"
     CDS             complement(4596..5432)
                     /locus_tag="OO585_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6630966.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5432..5707)
                     /locus_tag="OO585_003507"
                     /pseudo
     CDS             complement(5432..5707)
                     /locus_tag="OO585_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5782..6255)
                     /locus_tag="OO585_003508"
     CDS             complement(5782..6255)
                     /locus_tag="OO585_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6630967.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6276..6779)
                     /locus_tag="OO585_003509"
                     /pseudo
     CDS             complement(6276..6779)
                     /locus_tag="OO585_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1582 a         1755 c         1771 g         1671 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttttacatca ttccttcagc tcatacgtca
     2641 ctttcatcct ctgtgccatt tgtacatcac ccccttcctg aacgtgctga cagcgggttt
     2701 tccggctgcg ccggcaacgc ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt
     2761 tcggatggga agaacgccgg aaacaggact taccccggct gaatggtgtg aatttccgcg
     2821 ctatgcactt gtgcgcatac tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg
     2881 ggttttcggg tggtttgttg cctgttttac cggtttcccg tcagaaacgc cctgagggcc
     2941 tctcaggcgg tgctgataac agatgttatg gtaaatcaga gtcacctgca ctgttactct
     3001 gaaccggagc gaaacagccg cgaccagaat ccccgctttt tttcctgtgc cagcgcgtca
     3061 gccagacggc taatttcggc ctgtagttgt tccctctcct catttcgcag gcggttttct
     3121 gcttcccgcg ccatcttgtc ttcaagcatt agggtaacag tctgctgcag agcgtccagt
     3181 ttctgctgaa tgacgtctag ctgagcattt tccgcctggc taacgacaga tttggagtgt
     3241 ccctccttgt gcctttttgg tgtctcagaa gtgcgcagct cgccataggc ccgaatcagt
     3301 tcactggtct cgaactcccg gcgatcattt tttcctgtgc gatagctgac aaggccttta
     3361 gccatgtcac gatataattg cgagcgcgac tttcctgtta atttcagtgt ctcttgaact
     3421 gtgtgccatg ccataaaagt gacagtgtct cctgtgtgta ccgtattgta acagcagaac
     3481 atattactgt tttaacacaa aaggtctgag gctgcccgtg aggcgtgaaa aatacacaaa
     3541 aaacctgttg acggtgtggt tttgacgggc gtagtatttc gaatactgta tataaacaca
     3601 ttgttataaa tacagttttt gctgttttta aaagtcaaag aggataattt tatgagtaca
     3661 gagttaaata gcggtactgt cggtggtttt ggtcacatgg gaccaaatga cccctacaat
     3721 gatggtcata gcggctctgg aagttcggga ggtagtttta acggtggtgg taatggtggc
     3781 catgataata gtagtggttc atcttatact gcatcctttg gtctcatcac tggccgacac
     3841 ccgataactg tcccaggtac gaagaatatt aattctgtcg atctttctgc tgtcattaat
     3901 aacattatac atcatgaagg tgcacgcaat aacatgtacc tagatactgc gaacaatgta
     3961 agagtgggag tgacgggcac tggctggcaa tgtctagcaa cggcaggcat ttcggctgag
     4021 ggtaaaagaa ctttccgcta agcgatagac tgtatgtaaa cacagtattg caaggacgcg
     4081 gaacatgcct catgtggcgg ccaggacggc cagccgggat cgggatactg gtcgttacca
     4141 gagccaccga cccgagcaaa cccttctcta tcagatcgtt gacgagtatt acccggcatt
     4201 cgctgcgctt atggcagagc agggaaagga attgccgggc tatgtgcaac gggaatttga
     4261 agaatttctc caatgcgggc ggctggagca tggctttcta cgggttcgct gcgagtcttg
     4321 ccacgccgag cacctggtcg ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg
     4381 ggcgcggcgg atggccgaaa gtgccgcctt gctggttgat gaagtactgc ctgaacaacc
     4441 catgcgtcag tgggtgttga gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg
     4501 cgggaagggg cggaatccta cgctaaggct ttggccagcg atattctccg gtgagattga
     4561 tgtgttccca ggggatagga gaagtcgctt gatatctagt atgacgtctg tcgcacctgc
     4621 ttgatcgcgg ccgcgatagc tagatcgcgt tgctcctctt ctccatccgc gttccaagct
     4681 gcggaaaggc acccataagc gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc
     4741 gcacgagaga atgcgtccgc catctgtgca atgcgtctag gatcgagaca aaggtcgtct
     4801 ctgtcagccg gatcgtagaa catattggcg gcgccaaagc ccacttcacc gaccagaccg
     4861 acgggatcta tcaccagcca gccgcgactg gagaacatga tgttttcatg atgcagatcg
     4921 ccatgtagcc cacgcagttc cgaggcattg ctcatcattt gatcggctat aatcgccgcg
     4981 tggacgtagt cagtttgaca acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct
     5041 gcaaagcgat cccggatcgg gagaagggca gaaggcaggg gttcctcaga tgcggcatac
     5101 agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg
     5161 atgtgagaga gcattcgctc cccggcatat tcgagcaaca tcagattgtt ctcacgaccg
     5221 agcaaccgga ctgctcccct cccattgcgc cataccagat agtcggcccc gcgcagttca
     5281 tcagcaatgt cttctatagg tttcaatccc ttgacgattg caggagtccc gtctggcaat
     5341 gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc
     5401 caatgagcag gaaaaacagg cggcatgaac atcaacccca agtcagaggg tccaatcgca
     5461 gatagaaggc aaggcgttcg cggtcggggg cttcgatccc caatacattg aataggacag
     5521 cgaaggcgcg ctctgcttca tctggcgctg cccagttctc ttcggcgtta gcaatcatga
     5581 gtgccaaatc ggcatagcga tctgctgttc cgagccgccc aaggtcgatc agacccgtgc
     5641 attgaagagt tttagggtcc accatgaagt tcggcatgca gggatcacca tggcaaacaa
     5701 ccatatcgtc gctatcaatc gcggaaaaag gcgtagtgcg cgaagcgaac gaagcttttt
     5761 gccgtcgatt gcatagcttt gttaaccctt tttccaaatt tgatagcaat agttaatgtt
     5821 tgaagtaaag tgttgctcaa aaacaacttc gaaggtattt ggaatactcg ggaagaaaac
     5881 atccccctct ggctcgatgt cgatcgtcga taagtggagc gtagaggcca tgggtaatgt
     5941 ttctcggtaa atttctccgc caccagacac tataacgtga ccggtgaatt cagctagcct
     6001 gtccatggcc tcttcgattg actgaaatac aactacattg tcatcatttg atgtccaacc
     6061 tgagcgggta acgaccgcgt atttcctatt ggggagtgcg cccatagatt caaacgtctt
     6121 gcgacccacc agaagccact gattgtaggt caatgcttta aaaagtagct gctccccttt
     6181 cgcggaccag ggtatgtctg gaccgcaacc aatcacgccg tttttcgctt tcgcagctat
     6241 caatgatact ttcaaggttc tcatcctggg ttaacggtgc gctcttggtc gagccgcacc
     6301 ggtagctcta gttcgacacg agccaaaaga tcgagctgcg gcgtactctt gtcctcgtcc
     6361 ggtaagaagt cgggattgac ggcattgcgg gacaccacat caacggcgcg tccgaacatt
     6421 cgcgacagcc tgcgctcaaa cggacattga tcaaccgata ggctgtgaac agcgccaagt
     6481 tgctgcccca ttgacggcca cgctttgagc aaatccgctc cagacagatc agccgccggt
     6541 actcccggaa ttgccgttat caccaagcat gcaccctcct gttcctcctg ccagttgatg
     6601 acctcggggc aagccacacc tcgacctttg agccaaatga ggcggtcacg ctctccagcg
     6661 agctcaccgc ggcgggaagc aggtgcgatt ttcgcgaagg catgcccgtc accacgtcga
     6721 aaaacaaaat caccagattc tccgcctctg acaggcaacc agtcagaatg cgattcacc
//
LOCUS       DAJTYD010000188         6760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTYD010000188 DAJTYD010000000
VERSION     DAJTYD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.190"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..298
                     /locus_tag="OO585_003510"
     CDS             <1..298
                     /locus_tag="OO585_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6630968.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(449..3772)
                     /gene="mscM"
                     /locus_tag="OO585_003511"
     CDS             complement(449..3772)
                     /gene="mscM"
                     /locus_tag="OO585_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6630969.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(3794..4762)
                     /gene="psd"
                     /locus_tag="OO585_003512"
     CDS             complement(3794..4762)
                     /gene="psd"
                     /locus_tag="OO585_003512"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6630970.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(4859..5911)
                     /gene="rsgA"
                     /locus_tag="OO585_003513"
     CDS             complement(4859..5911)
                     /gene="rsgA"
                     /locus_tag="OO585_003513"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6630971.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6006..6551
                     /gene="orn"
                     /locus_tag="OO585_003514"
     CDS             6006..6551
                     /gene="orn"
                     /locus_tag="OO585_003514"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6630972.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1563 a         1817 c         1814 g         1566 t
ORIGIN      
        1 gcttccttac ctgttcctcg cgctggcttt cccattcttt aaagcacgtc aggatctcga
       61 cagaccattt gtgattttca aaacgcgtat gtcggcaatg attgcgacgg tggttgtcgt
      121 actggtggtg acatttgcga acgtcttcac cattattccg cctgtggttg aagccggaga
      181 ctgggacagc acattgtgga tgattggcgg ccctgtcttc ttctcgctgt tagcgatggc
      241 gatttaccag aactattgca gtcgcatggc gaataaacct gagttagctc tcgactgatc
      301 atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt tgtcaggaag taatgcgtat
      361 ggatgatact ctccagggcc ggtgataact gaagatgatg ttaagctgct aaatgagcca
      421 cgttgacatc atttggcagc ttcatgcatt acaaacttcc cgcctgacga cctttgcccg
      481 cagacgtcag cgttctcccc gtttgtttac cgttgaggct ttccagacgc atctggaagg
      541 gcgggaatgg catatcgata ccgtgggcat ggaagccagc cagaatcagc tggtggatct
      601 catggcgtag cggcatacgg tgacccatct cagcggcata aatacgcagc tcgaaaatct
      661 gaatcccttg ttgcagatcc accaggaaga cttccggtgc cgggttgtcg atcaccagcg
      721 agcagcgacg cgctgcggtg agcaggattt ccgtcacttc ttcactgttg gcatcagcag
      781 gggccggaat cgtcaacact acgcgcgtaa ccgagtcaga gagcgaccag ttgataaact
      841 gctcggtaat aaacgccttg ttcggcacga ttatctcttt acggtcccag tcgctgatgg
      901 tggtggcgcg ggtgttaatt ttcgtcacgc taccggtgag atcgcgaatt gtcaccgtat
      961 cgccaatgcg aatcggtttt tcgaacagga taatcagacc agagataaag ttggcgaaaa
     1021 tttcctgcaa accaaaaccg agaccaacac cgagcgcggc gaccagccac tgcaatttcg
     1081 accattcaat accaatcatc gagaagccga ccagcccgcc aatcagcatc agcagatatt
     1141 tggtgatggt ggtgatggcg tagcctgtac ccggcgttaa atccaggtgc tgcaaaatcg
     1201 ccagttccag cagcgcgggc aggttgcgca ccagctgcgt ggtgatgata aacaccagaa
     1261 tggcaatcag caccgcaccg agggtaattg gctccagact ttctacgcct tgtaccgtgg
     1321 aggtgacatc ccacagcgaa atattttcga ggaagccgaa agcggaatgg atttctgacc
     1381 acagcacaat gacagaaagc agggcgatca acatcaaaat tgagcgcacc agccgcaagg
     1441 attgcgcact gatggcatcg agatcgactt cgctttcatc gacttcaatt gctccttccg
     1501 ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg ttgcgctaac atctctgccc
     1561 ggcgatgctt cgcccggtca aacgccagcc tgcggcgctg gatcagcatc cagcggcgga
     1621 taacgtgata aaccaccagt agcaggaacc agatggcaac cgaggtttca agcctcgcta
     1681 acagcgcctg tgccgttgcc agataaccca cagccgacgc cagaatggca accaacggcg
     1741 cgccaatcat catgttccac agcatatggt tggtaatgtt gtcgccgctg ccctctttgt
     1801 tgagatacag tgggatcccg gcctttttca ggctgagggt gaccaccgcc agcgcaccgc
     1861 aaatgaggat aaagcaaagc cgtcccagcg aaccggagaa ttcacggtcg tcgaggttat
     1921 cgaacatcat cagcgccata atcagcggca caataagccc gatgctcatc aggtagtagc
     1981 gcatcccacg ggaaacacgt tcgcgcggcc agccaaaatg agcgataaac aagccgttcg
     2041 ggcgggcaaa ggtggcgcaa atcatcacta cccacagcag cggcacggta gccgttacac
     2101 catcgccaat cgcgaccgcc agcggataag gccacgcctc gcgcaagccg taacccagcg
     2161 tcatccacag caccggtaac ggtgacgcga cgagaatcga ccagaaaaga gtgcgcaacg
     2221 tcagccagaa gtgatcctga gtcactttgc caactttcgc cgccgaacgt tcaagaaaac
     2281 gggtgaaata gcggcgcgag taaatactgc aaccgaccag aatcaacgcg ccaaacagcg
     2341 gcaaaattgt ctctttgctg gtcagcatca tcacactggc tttgcccaac tgactgaagg
     2401 tgtccagcga aatgagacga cgcagatcct gggcgatttc cagcggccag gcgatggtca
     2461 tcgggcgcac gtcagaggtc cagaacagat agcggtgcgt tgcttcgttc acctctttca
     2521 gcgcatcctc cagttgcccg ttggagactt tcagcttggt cagttccaat agtagcgtgt
     2581 cgccaccctg caacaatgag ttcagcaact cacgctgagt gcgcagttgt gcttccagaa
     2641 tacggttttg ctcggcggtc agcggctgac cgtcggcctg atggatttgc cgtagcagcg
     2701 gctgtttatt aagcagatcc tcataacgta accgttgcac acgcaactgc gccatttcgg
     2761 tatcaagctg ttgtggtttt ggcatttctg gcagccgtgc cacctgcgcc cgcagcgctt
     2821 cgccgagcag attggacgat cccagccatt gcgactgttc acgcagcgta ttcaacgcct
     2881 gccggacctg taacgtctgg ctggcagcct gacgctgttg cgaggcaacg agatccatcc
     2941 gctgcgcctg ttgattcaaa gccgccgata gttcgcggtt aattttgaat tgcgcgacga
     3001 tatctttcgg caaatcggcg ctgttttctg ccagcaattc ggtactttcc agcgcccgct
     3061 ccgcctcaag ctgacgttgg ctgtttaatt gattacgcaa ggcctgcaaa tacgcatcca
     3121 gttgctggct ctctttttcc gccagctctg agcgtaagcg cgctaattcc tggcggttat
     3181 tggcagacag ctgcgccagc tccagttcat caacgagcgc cttaagacgt gcagagtcag
     3241 actgcaacgc gaaattttgt gcctgattga gcggagtatt gccggtaagc gttcccaggc
     3301 ggcgctcgat ctcatttaac tgacggcggg cgtcggtttg ctgttgcggc agttgattca
     3361 gcgaatcggc aatctcgcgg gtgcgctcct gctcttgctg ggcctgacgg cttttatcca
     3421 gcaactggct gctgacctgg agaatttcct gattcagcgc gtcggtagac attcccggcg
     3481 acacgctgcg cggctcgtca cgcatgttgt ttaattgtgc gcgcagagta gcggagagtt
     3541 tcggataatt atcgataact tgctgatatt gtttgatgcg ctcaagggaa ccttttcgtt
     3601 cctcaagcgc atttaaggca gactggagcg cctctacgac ttccggctgt gcgggtttcg
     3661 ccgcttttgc ctgctccagt tcctgagtga tttgtttgct atcgggggcc gtcgcggcgt
     3721 acgcccccca actgaggcac caggccatca gaaaagtgat aatcaggcgc acgtcagcgt
     3781 ttcctttgat ggattagacc tggtcttttt tatcgtcaac caatgggctg gcgtcgtgtt
     3841 ctgcttcgat ctcttcagca ggaagtgggg cgggttcagc gtctggcgta acaaaggttt
     3901 cggtagatac tgccagcggc tggccaattt tcgtgacaga caggctttcc agttgctcaa
     3961 ccagattcac tttacccggt gcaaacaggt tgataacggt ggaaccgagt ttaaagcgac
     4021 ccatttcctg gcctttcagc agtgccacag aaccgtcgtt ttccccggca ggccaggtcc
     4081 agcgcttgat gataccttcg cgcggcggcg taatggtgcc cgcccagacc gtctcaatgc
     4141 tgccaacaat cgtcgctccg accagaatct gcgccattgg gccaaattcg gtatcgaaaa
     4201 ggcaaatcac gcgttcgtta cgggcaaaca gattcggcac gttctgagcg gtgagatggt
     4261 taacggagaa gagatcgccc ggcacgtaga tcatctcacg cagaataccg ttgcacggca
     4321 tgtgtacgcg atggtagtca cgcggggaga ggtaagtggt cacaaacgta ccgttgcgga
     4381 acaggtccgc catcagatag ttgcctgcca gcagggcttc gaggctgtag ttgtggcctt
     4441 tggcttgcag gattttatct tcttcgattt tacccaactg gctgataacg ccatcggcag
     4501 gcatgaccag tacattcgga tcggtatcga ttgggcgtac ttcgtcacgc agcggacgga
     4561 caaagaattc gttaaaggtg cggtagctgg cggtgtccgg cttttgcgcc tctttcatgt
     4621 cgaccttgta gtatttaacg aacagatcga taaccagttt tgtcagccat cctgcccgct
     4681 tgcttgcgcc ccaacccgcc aggcgagtaa gccatagttt cggcagaatg tactgtagcg
     4741 aaagtttaaa tgaatttaac aaggtagcct ccaggccatt gttttgtcgt tcctgatccg
     4801 gccaacatgc cggatcctga aaaaaagggg acgattctaa cgacggttag cttaattgtc
     4861 agtcatccgt atcagaaaag tttttacgcg tttttacctg cgccatgctt tccagaatac
     4921 ggtgatagtt ttcgaaacgg gtttccgcga ttttcccttc ctcaaccgct tcccggatag
     4981 cgcagcccgg atcggtatcg tgtttgcaat cgcgatattt acacagacct aaatagtcat
     5041 ggaattcgac aaagccctga gtgatttgtt ccggctccag gtgccagagg ccgaactcac
     5101 gcacaccagg ggaatcaatc acatcaccgc cgtgcgggaa gtgatacagc cgagcggcgg
     5161 tcgtggtgtg ctgaccgagc cccgagttgt cagagacatc gttggtcagg atctcttttt
     5221 gcagccccag caacgcattc agcaggctgg atttaccgac gccagactgc ccggcaaaaa
     5281 tgctgatgcg cccggtcaac gcctcttcca gcggttttag cccatcctga gtatggctgg
     5341 aaaccatcaa tacgcgataa ccgatattgc ggtagatatc catctgctcg ttgacgaacg
     5401 ccatgccttc gtcgtccagc aggtctatct tgttgagcac aataatcggc tcaatctgca
     5461 aggtttcgca ggccaccagg taacggtcga taatattgag tgacagctcc ggcaaaatgg
     5521 cggagacaat gacaatctgg tcgatgttgg cggcaatagg ttttacgccg tcgtagaaat
     5581 ccggacgcgt caacaccgag gtacgctcat gcaccgcttc cacgatccct ttgacattta
     5641 cgccttccgc cgccggttta cccggacgcc agactacacg gtcgccggtc accagcgaac
     5701 ggatggtacg gcgaatattg cagcggtgaa cgtcgccatc ggcggattcc acatcagcgt
     5761 gcataccaaa gcggctgatg acgatacctt catcaggctc gccaaacaga ttgtcgtcgt
     5821 agtcgggctt ctccttagac gttttaagac gacgctggtg attggcgttc acgcggcgct
     5881 gctggccttt ggagagttta tttttactca atcgtacaga ctcctggtcg cccctgatgg
     5941 gcaaaacatc tatgatacac gcaattgtgg atcaatatag tcactgtgaa tgggtggaaa
     6001 atagcatgag tgccaatgaa aacaacctga tttggatcga tcttgagatg actggtctgg
     6061 atcccgagcg cgatcgcatt attgagattg ccacgctggt gaccgatgcc aacctgaata
     6121 ttctggcaga agggccgacc attgcagtac atcagtccga tgaacagctg gcgctgatgg
     6181 atgactggaa cgtgcgcacc cataccgcca gcgggctggt agagcgcgtg aaagcgagca
     6241 cgatgggcga tcgggaagct gaactggcaa cgctcgaatt cttaaaacag tgggtgcctg
     6301 cgggaaaatc gccgatttgc ggtaacagca tcggacagga ccgtcgtttc ctgtttaaat
     6361 acatgccgga gctggaagcc tacttccact accgttatct cgatgtcagc accctgaaag
     6421 agctggcgcg ccgctggaag ccggaaattc tggatagttt taccaagcag gggacgcatc
     6481 aggcgatgga tgatatccgt gaatcggtgg cggagctggc ttactaccgc gagcatttta
     6541 tcaagctgta aaatttttcc tggtcacgta agcgccggaa tccacttgcg gcggcgtgat
     6601 tttgacgcta aattgaacgt cttgctgata atttcagcac ttgagataaa aacgcaaaaa
     6661 aaactttttt ggggggttgc agagggaaag atttctcgta taatgcgcct cccgtaacga
     6721 cgcagaaatg cgaaaattac gaaagcaaaa ttaagtagta
//
LOCUS       DAJTYD010000189         6682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTYD010000189 DAJTYD010000000
VERSION     DAJTYD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.191"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..2688
                     /gene="ehaG"
                     /locus_tag="OO585_003515"
     CDS             <1..2688
                     /gene="ehaG"
                     /locus_tag="OO585_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCR6630973.1"
                     /translation="FTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATY
                     FGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSA
                     KHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAP
                     TYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANG
                     AISAASSDAINGSQLYTTNKYIADALDGDAEVNADGTITAPTYTIANAEYNNVGDALD
                     ALDDNALLWDETANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMM
                     IEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVA
                     KGDSSVAIGQGSYSDVDTGIALGSSSVSSQVIAKGSRDTSITENGVVIGYDTTDGELL
                     GALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLA
                     VGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTT
                     TRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDER
                     VAQNTQSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSV
                     AIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLK
                     SSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSVQE
                     TKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGES
                     AVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3056..4711
                     /gene="lldP"
                     /locus_tag="OO585_003516"
     CDS             3056..4711
                     /gene="lldP"
                     /locus_tag="OO585_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6630974.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4711..5487
                     /gene="lldR"
                     /locus_tag="OO585_003517"
     CDS             4711..5487
                     /gene="lldR"
                     /locus_tag="OO585_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6630975.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            5484..6674
                     /gene="lldD"
                     /locus_tag="OO585_003518"
     CDS             5484..6674
                     /gene="lldD"
                     /locus_tag="OO585_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6630976.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1553 a         1752 c         1788 g         1589 t
ORIGIN      
        1 ttcactgcag ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca
       61 gtcactgcaa ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat
      121 atcgccacct acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca
      181 acctacaaaa tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac
      241 tcctcattta gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc
      301 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
      361 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
      421 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
      481 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
      541 accactcttg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
      601 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
      661 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
      721 gctgatgcgc tggatggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
      781 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ctctggatgc gcttgatgat
      841 aacgccctgc tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat
      901 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
      961 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     1021 attatcaatc agctcgctgg taacaccgac gcaacctata tccaagaaaa cggtgcgggt
     1081 attaactatg tgcgtactaa cgacgacggc ttagcgttca acgacgccag cgcacagggt
     1141 gttggcgcta cagctatagg ttataactct gtcgccaaag gcgatagcag cgtagctatt
     1201 ggtcagggca gctacagcga cgttgatacg ggtatcgccc taggtagcag ctctgtttcc
     1261 agccaagtga ttgccaaagg ctcccgtgac accagcataa cggaaaatgg cgttgttatt
     1321 ggttacgaca ccacggatgg cgaactgctc ggtgcattgt ctatcggtga tgacggtaaa
     1381 tatcgtcaaa tcatcaacgt agccgatggt tccgaagccc atgacgccgt tacggttcgt
     1441 caattgcaga atgcgattgg tgcggtcgca accacgccga ctaaatactt ccacgctaat
     1501 tcaacggaag aagattcact ggcagtggga actgactcgc tggcaatggg tgcgaaaacc
     1561 atcgtgaatg gcgataaagg tattggtatc ggttatggtg cctacgtgga cgcgaatgca
     1621 cttaacggca ttgccattgg tagcaatgcg caagtcattc atgtcaacag tattgcgata
     1681 ggtaatggtt ctacgaccac tcgtggcgct caaaccaatt ataccgccta caacatggac
     1741 gcaccgcaga actctgtcgg tgaattctca gtcggtagtg cggatggtca acgtcagatc
     1801 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     1861 actgatgagc gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact
     1921 aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt
     1981 agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat
     2041 agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc
     2101 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     2161 atcaccaacg tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     2221 tcttctgaag caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc
     2281 aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc
     2341 gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag
     2401 caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     2461 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     2521 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     2581 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     2641 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     2701 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     2761 aggcgctccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac
     2821 cagacatctc cccccacaag aattggccct accaattctt cgcttatctg acctctggtt
     2881 cacaattccc caattaaaac tcacatcaat attgccaata cacaacattt agttaaccat
     2941 tcattgtcat tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca
     3001 aggagatgag caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa
     3061 tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc
     3121 gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc
     3181 cgcctcatgg acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt
     3241 cgctaacgcg ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg
     3301 gatcattatt gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat
     3361 cattcgctcg tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg
     3421 tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac
     3481 cgccgcattg ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat
     3541 tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca
     3601 agtaacaggc atcgacagtt ttgagattgg ccagatggtg gggcgtcagc taccgtttat
     3661 gaccattatc gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaaaga
     3721 gacgtggcct gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc
     3781 caacttcatt gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct
     3841 gacgctgttc ctcaaacgct ggcaaccagt tcgcgtattc cgcttcggtg acttgggggc
     3901 gtcacaggtt gatatgacgc tggcccacac cggttacacc gcgggtcagg tgttacgtgc
     3961 ctggacaccg ttcctgttcc tgaccgctac cgtaacactg tggagcatcc cgccgtttaa
     4021 agccctgttc gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta
     4081 cctcgataaa ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc
     4141 cgtgtttaag tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct
     4201 ctcgattgtc tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct
     4261 gaaagaactg gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc
     4321 gaactattcc ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt
     4381 caccttcttc tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc
     4441 atctaacgcc ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga
     4501 tctgttgctg gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca
     4561 atctatcgct atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg
     4621 ctttactgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca
     4681 ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac
     4741 gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag
     4801 ttgcccgctg agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag
     4861 gcgctggcaa aactagtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt
     4921 attcgctggc gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg
     4981 atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc
     5041 agcaccgcat ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt
     5101 tgctttgaag caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt
     5161 catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc
     5221 ttcgatgtcc tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg
     5281 gttttttcac aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat
     5341 gccgacgggg cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa
     5401 cgattcgatg aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat
     5461 gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag
     5521 cgcaacgcat tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat
     5581 acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga
     5641 aaaacatgtc cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg
     5701 tagcactggc accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag
     5761 ccaaagcggc ggacacgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga
     5821 ttgaagaagt cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg
     5881 atcgcggctt tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg
     5941 ttttcaccgt ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga
     6001 gcggcccaaa tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt
     6061 gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca
     6121 aaccaaccgg actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct
     6181 catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga
     6241 tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt
     6301 ctaaccacgg tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta
     6361 ttgcagatgc ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc
     6421 ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc
     6481 tgtatgcgct ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa
     6541 aagagatgaa agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag
     6601 attcgctggt gcaagggctg ggtaaagagt tgcctgcggc actggctccc atggcgaaag
     6661 ggaatgcggc atagtcgttt gc
//
LOCUS       DAJTYD010000190         6505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTYD010000190 DAJTYD010000000
VERSION     DAJTYD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.192"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(89..775)
                     /locus_tag="OO585_003519"
     CDS             complement(89..775)
                     /locus_tag="OO585_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6630977.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO585_003520"
     CDS             complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO585_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6630978.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO585_003521"
     CDS             complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO585_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6630979.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO585_003522"
     CDS             complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO585_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6630980.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(5228..6056)
                     /locus_tag="OO585_003523"
                     /pseudo
     CDS             complement(5228..6056)
                     /locus_tag="OO585_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
BASE COUNT         1681 a         1729 c         1483 g         1612 t
ORIGIN      
        1 tttaaagaag agctcgtacg aaggtacggg ctttttttcg tatattgcac acaccggggg
       61 gatgagaagc cccgaccggg atacttagtt aatgcaccgg ttcactcttt gtcgccgcga
      121 ctaatgggcg aattttgtca cgagtctccc agaccggagg aatttcctcc aatttagtga
      181 cagaagctcg tacaaaaggc acccgtgcaa gagtcataat tgtatccaac caacggtcac
      241 gaataatgcg atccgccctt tcatgactcg ggtcatccag ctcaataaca cagactattt
      301 tattgttctt ctttgaaacc agaacataat ccattgccat gccattataa attgcaaaga
      361 aacgctttct ttcttcttcg ctaaactcag gttgttctgg aaagttgatc aagcgcccca
      421 gagagacctg gcaatggata tgacaataat gtccaaacca tttttccaga ttatcgaaat
      481 acattttttc acaacgagtg attaaccact cttttctttc caataaaata ccttcggcaa
      541 ccattccctg aaataactca tctcgctctt gccaacgaga gtcacgatag gttttctgcg
      601 gtatatagct ccttgtcgac gccttagcgg caggggaagt tgcagatgaa gggacgggtt
      661 gcgatttagc gatgattacc gatgaggcaa cttttcggag tgtcaatttt ctggcaacag
      721 caaaaccggc gataaatgca attgtcaaac aagttacaaa agccataaag ctcataatta
      781 attttcacct atccatgttg attatatttc attaccaatg ttactcgctg cagcgtgtta
      841 catgccaaca atgcaacgat caatatatag ttagtatcat gttttgatta ttacaaccat
      901 ccctatttag ccattgcggc ttgccttcca caaaaaaata acaacacaca atattagcaa
      961 agaattaatt aagattaacc ttacatttat aatattatcc ctaaagaata aatcagagga
     1021 atatattcgg tgttaaacag tcagtcaaag tatagcccat tgttgattaa acctcataag
     1081 caaaattggc accagatctc ctgtacaggt tgaatggccg aaaaaagata ggcagatgct
     1141 catttttttg gcttctggaa gaaaaactct ccgcgctggc agtattacac tggtctgatg
     1201 ctatgacgaa aaatgcctgg taagagtatc taaaccagac tccagttcag cttatcaaca
     1261 cgctcaaagt cgcccccctg aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa
     1321 gcccggttcg cccgggctct gcaccgataa cacacttaac tgtatgcatg cagcgtacgt
     1381 tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca
     1441 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     1501 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     1561 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     1621 aaggtacgtc cacgcatata ggcgaacggc gcgatttcca ccttccctat ttccggtcgc
     1681 aggcagtact gcataaagga agcccctaag cgccggacca gcacgtcgta gaccgggcga
     1741 aaatagggag caaacttttc tgcgatatca ccaggtaaga agccaagatc ttcatcggct
     1801 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc
     1861 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     1921 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     1981 gaggtatcgc gactgtcgcg ggccatacca atggcttcta cgccgctcat ctgcacaagc
     2041 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     2101 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     2161 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     2221 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcgca
     2281 ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag gttgaaaatg caaagtgacg
     2341 agatgactac cggaggagaa aactccgcga gtggtggcgc gttgattatc taaaacatgt
     2401 ccagtacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     2461 tccgctgcta cttaataagt acaacagatc tcgcatttta ttgcaacaat atatttactt
     2521 atatttaact ataaaacact atttcagtga cattagtttc tactggaaag atgacagagt
     2581 gatgacagtg atgaaaaaag ctgtgtgctt tcagcaggat ttgcatcagg catcagtgca
     2641 cgatcgccgg atgcggcgtg aacgccttat ccagcctacc aaacccgcac tgattgtagg
     2701 catgataaga cgcgcaagcg tcgcatcagg catctgcgca caaatgcctt atccgcctaa
     2761 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     2821 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     2881 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     2941 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3001 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3061 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3121 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3181 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3241 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3301 ttatcattct ggctatcggg attggcagtg ccgtctttaa agcccagcaa attaatcggc
     3361 gtctctttgc ctttactacg cgctgcgtga tcggaaataa acccttcccg cttccagcgc
     3421 acgctgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     3481 tgggtgttgg cgcaaatttg tagcaacaca tcaccatggc ataacgccgc atccagcgag
     3541 tcgttgggga aacgcgtcat cttctgcaac tttttcggca tctgtggcgc aaggccaaac
     3601 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     3661 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgcc
     3721 gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgttcaaga
     3781 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     3841 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     3901 gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     3961 agcgccagcg cacctatcac tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     4021 tcatctttat actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat
     4081 cttccgcctg cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     4141 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     4201 agttggcatc gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca
     4261 aatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     4321 tgtagcgatc ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg
     4381 cgccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     4441 ccacatctgt ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa
     4501 acaatgcttt ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt
     4561 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
     4621 caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
     4681 cagctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca
     4741 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
     4801 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac
     4861 cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaaaccag
     4921 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
     4981 ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
     5041 tcggttcgca ctgcttatcc gtcacggtca ctttgacctg cggcacatca gcggcgttag
     5101 ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcagaagt
     5161 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
     5221 gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcata gcaaatgcca
     5281 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggcgcttcct
     5341 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcaccgcac
     5401 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
     5461 tggcaccagc tgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
     5521 atgcaccaag attgaggcga ataccgcccc agtagagcag aaagccgagc accacggctg
     5581 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
     5641 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
     5701 ccagcgccca gccatgatga tttccacgct acaatgcgct atcgactgcc tgttccagtt
     5761 gcacttacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
     5821 gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg
     5881 gtttcgttaa tgaagatccc caggcccagg cacaacgcag cggcaagcaa cacgccaatc
     5941 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
     6001 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
     6061 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
     6121 cgattatcat tacggtaaag aaaaatacaa gcggaatgtt tataaaatga tgttggaatg
     6181 taaacaaaga gaggtaatgc caggggatat aaacgattat tccagggtag aaagatggga
     6241 ttgtttgcaa agaaccatgt gttcttgccg gatgccgcgt aaacgccata tccagccgac
     6301 aaaactgtcc agactcaatg catcagagct tccatgtaaa actgataagc gggtgtatca
     6361 agcaatttcg tccatccgcg attatcaaat ctaacttttt gcccggaaca taataacagc
     6421 tcgggtgttg gtggcgtcaa aatcgctgaa gcgttccccg aaggccggga cagcccacat
     6481 ggatgtgggc tgagggcgcg tctta
//
LOCUS       DAJTYD010000191         6253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTYD010000191 DAJTYD010000000
VERSION     DAJTYD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.193"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..178)
                     /locus_tag="OO585_003524"
     CDS             complement(<1..178)
                     /locus_tag="OO585_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6630981.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSP"
     gene            complement(172..1410)
                     /gene="preT"
                     /locus_tag="OO585_003525"
     CDS             complement(172..1410)
                     /gene="preT"
                     /locus_tag="OO585_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6630982.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1807..2046)
                     /locus_tag="OO585_003526"
     CDS             complement(1807..2046)
                     /locus_tag="OO585_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6630983.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2049..2768)
                     /gene="sanA"
                     /locus_tag="OO585_003527"
     CDS             complement(2049..2768)
                     /gene="sanA"
                     /locus_tag="OO585_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6630984.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(2918..3802)
                     /gene="cdd"
                     /locus_tag="OO585_003528"
     CDS             complement(2918..3802)
                     /gene="cdd"
                     /locus_tag="OO585_003528"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6630985.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPYAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(3932..4627)
                     /locus_tag="OO585_003529"
     CDS             complement(3932..4627)
                     /locus_tag="OO585_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6630986.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYGSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4624..5022)
                     /locus_tag="OO585_003530"
     CDS             complement(4624..5022)
                     /locus_tag="OO585_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6630987.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5261..6211
                     /gene="dusC"
                     /locus_tag="OO585_003531"
     CDS             5261..6211
                     /gene="dusC"
                     /locus_tag="OO585_003531"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6630988.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         1630 a         1587 c         1576 g         1460 t
ORIGIN      
        1 gcggcgagac ttcgttggcg ataaaaaagc cgatcgtttt aaacaccaca ccgccccaac
       61 ctgtgtcgta ggctttggca cacatctcat agcagttgcc taccggcgaa gaagagaggc
      121 agaacgggtt gggaaacttc acgccgcaaa aagtaatcga aagatctttc gttaacatga
      181 gcaagctccc tctaaatagt gatgaatcgc ctcggcggct tctttcccgg ttttcacggc
      241 atagaccatg gttttgtcac cctcaacaat atcgccagca gcaaagactt gcgggtcgcg
      301 ggtctggtaa ttttgtgttt tgatggtgtt acgctgcggc tctaactccg caaaggcatc
      361 tagtctggca tgctgaccga cggcgagaat aattttatct gccgccatcg tcagttcgcc
      421 cgataaccgt acatgcttaa acgtgacttt attgccttcc acggctactg gcgtgaatcc
      481 atcgatgatc gaaacgccca gctcccgggc gctggtaaac tctttttcgc ttgccggaaa
      541 ctcatctaac tcttcacgcg ctacgcaagt taccgcctga cagccgagaa ctttcagcgt
      601 gctggctacg tccatcgtga catcaccgcc gccgataatt aatgcgcttt gtggaatgct
      661 gatatcgcct tgcgcctgtc gtgcacgttg caagaagtcg acggcaatct caacgtcact
      721 atgctcaaac agcggtagcc cggaaccgct tgataacccc acggtgacca gtaccgcgcg
      781 gttttctgct ttcagctgct caagggtgag tgtgttaccg acttcgttgt tgcacttaat
      841 ggtcacgccc attttttcaa tacgggcgat ctctgcatcc agtactgatt gcggtaaccg
      901 gaattgcgga atgccgttac gcaaccagcc accgggttgt gcttctttct catagatcgt
      961 gacgtcataa ccctggtttg tcagtgtcac actggcctgc aatccggcag gacctgcgcc
     1021 aataatcgcg actttgccga gcgttttagt accgggctga taaatttcca tcccggtttg
     1081 ttgttcaaaa tcagtaacaa aacgctgtaa gcggccgata tcaatgggag catcaacacc
     1141 ggcacgggta caaccgcttt ggcataattt ctccgtcggg cacactctgg cacaaacggc
     1201 accgagggca ttattttcgc gaattgtctc ggcagcgcct ttaaaattac gaaagtagat
     1261 tgagcgaata aatttccccg gatcggtctg tgccgggcaa gcctgactac agggagcgtc
     1321 gtgacataat aaacagcgag aggcttcttt aatcgccagt aacggcgtaa aagccggagt
     1381 gagttcatcc agataatttt gttgcggcat gtgatcttcc ttaatgctat gagtaattaa
     1441 ggcgaacgct aaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgggttatt
     1501 cggcccatcc atgggcctca ccccttatgg ggccgctgca ggcagcgttc aaatccgctc
     1561 catgcggatt tgtcgctcac cccagtcact tacttatgta agctcctggg gactcactca
     1621 cttgtcgcct tcctgcaact cgaattattt agagtataaa tattgcgtgc tgatattaag
     1681 gctatgcgcg tggggaaatt tatactgtga aaggattcac aaatagctgc ggttcagaat
     1741 gttatatccg cagcttattg agtgaagtaa taattattct caataattaa cagaaaagag
     1801 aaaatattaa cgatagaaaa taagataaac aatcatgccg atagataaaa ttccgaagcc
     1861 gatatatgcc accatataag cgaaaaagag tccgggattt tttgctcgcc agacatacac
     1921 cggttccggt gcatttttaa cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc
     1981 ttcgcggaaa caaaatatcg gaataaggaa aaaaacggca acgacaaaaa actgctgtac
     2041 atccataact actttccttg tttcttttgt aattcaagta actgttcggg tgtgacggcg
     2101 ggatacccct gcgcatcttc cggcacctgg tgcatagccg gaatagggac cagcggccct
     2161 aaaaaacgcg gttcacgttt aaaaatataa aggtcagcca gcgcaccgaa acgggcggca
     2221 aattcgcgaa tacgtactga cagcatatct ttcggtgacg gtacggcata acactgagcc
     2281 tgaatcccca tatgcagcgc aataaataat gcccgctcac agtggaaacg ttgggtgata
     2341 ataatgaaat cattagtatc gaaaactttg cgtgtacgca cgatggaatc cagcgtacga
     2401 aagcctgcgt aatcgagaac aatatctgat gggtcgacac cagcagcgat taaatctttg
     2461 cgcatggtca tcggctcatt ataactttgc aatgcgttat cgccgctcag taatagataa
     2521 tttaccttac cgctgttata ggcattaatc gctccttgaa tgcggtagcg ataatactga
     2581 ttaattacgc cagtacgata atattttgct gttccgagca ccacaccgac ctggcggtag
     2641 gggagatcct gcaattcgtc gtagatataa ggcgcggttt tccagctcat ccagcgatcg
     2701 aggccgagca cagtcaacaa cagcaagccg atcaggacta acaggctgag gaacacgcgc
     2761 tttaacatgc aggagactcg atctaaggaa aatttccatc aggctacttt acccgctggt
     2821 tttgcgcaag aaacggcaaa ctgtttgccg ggaatttcga caccaggaga gggctggcaa
     2881 gccggacaag gcgttcacgc cgcatccggc accaggctta agcgagaagc actcggtcga
     2941 tactgtgaca gccgagagct ttcagcgttg cggaggtggc atcccactga atcaacggcg
     3001 catcggcttt ttctgccaga accgcgcgct ggatatccgg gtaatcataa cccttgagat
     3061 tcaacagaat taacgctccc tgcaacggtg gcagagtcgg gttgaatgcg gcgttttcag
     3121 cgtagctgcc actgaaaata cgaccgtctt tacattccag cgcgacaccg cttggcgact
     3181 tactgtaagg catgtgcgaa cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac
     3241 cagtcagcgc atagccgtga tcctgctcgt ccatcagcag cgttttaatc tccagatctt
     3301 tcggcccaaa ggcatacggc agatagtcac gcagcgcgtg tgcctcgcgg cccggcagat
     3361 gaatacgcag atccagaccg ctgttcagtt cattcataaa ctgacggcag tgaccacaag
     3421 gcgtgtagtt aacggtgatg gctgcaagcg ctttttcacc actcaaccag gcgtggctga
     3481 tcgcgctttg ttcggcatga acggtttgct gcattgtcgc accaataaat tccatattgg
     3541 caccgaaata ccaggttccg ctcacaccgc gcgcaattgc gccaacatta aaattcgaca
     3601 atggcgtacg cgcacaggcc gctgccagcg gaagtagtgc gaatgccagc gcgtcttcgt
     3661 ccagccccgt tgcgctcttc agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt
     3721 acttgtctgc cagaataggt tccagtgcag attgcaagtt atccgcaagt tgggcaaaag
     3781 cggtttgaaa acgtggatgc atggcgttgc ctcataatca tgtaatggga taacagttta
     3841 cgaacccgtt atggctttat atgtgatctg aatctcatta attatgcaac gcgtgaaact
     3901 tacatcaaaa atgcgcgacg catcgcaaat tttagcccat tactgccaga ataatcggga
     3961 aaaggaacgg tgcgatcagc gaagtaatta tcccgcataa caccagcgct agcgaactaa
     4021 atgcaccttc ctgataatcc agctcggcgc agcgcgccgt accgagggcg tgcgaggcag
     4081 tccccattgc cagaccgcgc gcagctttgg tacgaatacg catcgcatta agcaatgtat
     4141 ggccaaatac cgcgccgagg atgccgacga aaatcacgca aactgcgctg attgccggaa
     4201 taccgccaat actgccgcca accgccattg caataggcgt ggtgactgat ttcggcagga
     4261 ttgacgcggc gatttccggt gaagcgccca tcaacaatgc cacggaagtc cccgtcacca
     4321 tcgcaaccac gctgccgata aaacagatgg tgatgatcga tttccagcgc gcgcggatct
     4381 ggtgtagctg ctcatataaa ggataggcca gcgcgacgac cgccggttgc agcagatcgt
     4441 ttagcacttc gctaccttta aagtagctgc cgtaagagat gccggtcagc attaaaaaag
     4501 ggatgatcac caccattgct accagcaacg ggttaagcaa gggaaactta taccgtgccg
     4561 ccagtttgcg ggcggcaaaa aagacgatca atgtcagtgg taatgaccac cagatattag
     4621 ccatcattct tctgatcctt tctgacctac gactttacgt tcaccgtgca ccagttgcga
     4681 actccagctc accaccagaa aaaccaccag tgtactgaca gcacaggaaa ccactaccgg
     4741 gccaaactgt gcgcggagca aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa
     4801 caatagcgcc atatagcgaa tcagtacgta gcaccccgga ttgacccatt ttgccggaag
     4861 aatttgcaag gccagcagga caaacaggat cagcatcccg atgatgctgc ccggaatggt
     4921 taccggtagc agggaagcaa tgaaaatgcc tgcatacagg caggcataaa tcaggacgaa
     4981 agcgcgtaaa tattgccaga taatgttcag tgtcttactc ataatcaaca ccccagatga
     5041 gatgaattca tcatacaatt aattcgtaaa acgtgctacc gatcacatta agaaatcagc
     5101 gcttattttt tcgctccccg gcattacgca atgcgcaacg aactcaggca tacatcaagc
     5161 atagagaggg attacagttt gcagcaatcc ctctttgcag acctttaatc cgttgaccat
     5221 aggcgctaac atagcgcctc atttttttgc gggtgaagat atgcgtgtgt tactggctcc
     5281 gatggagggt gtgcttgact ctctggtgcg tgaattgctg accgaagtta acgactacga
     5341 tctgtgcatc accgagtttg tccgcgtggt ggatcaactg ctgccggtaa aagtttttca
     5401 tcgcatttgc cctgagctac aaaacgccag ccggacacca tccggtacgc tggtgcgcgt
     5461 gcagttgttg ggtcagttcc cacaatggct ggcagagaat gccgcccgtg cggtggaatt
     5521 aggttcctgg ggcgtggatc tcaattgcgg ctgcccgtcg aaaacggtca acggtagcgg
     5581 tggtggggcg acgttactca aagatcctga actcatctac cagggtgcaa aagcgatgcg
     5641 tgaagctgta ccggcgcatt tgcccgtcag cgtgaaagtg cgtctgggct gggacagcgg
     5701 tgagaagaaa tttgaaatcg ccgatgcggt tcaacaggct ggtgctacgg agctggtggt
     5761 gcatggacgg acaaaagagc agggttaccg cgcggagcat attgactggc aggcgattgg
     5821 cgagattcgc cagcggctga atattccggt gattgccaac ggtgaaatct gggactggca
     5881 gagtgcgcaa caatgtatgg cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc
     5941 gctcaatatt cccaacctga gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc
     6001 ggaggtggtt gctttgctgc aaaaatatac ccgtctggaa aagcagggcg ataccgggtt
     6061 atatcacgtt gcgcggatta aacagtggtt gagttatttg cgtaaagaat acgatgaagc
     6121 aacggaatta tttcagcatg ttcgggtgtt gaataattcc cctgatattg caagggctat
     6181 tcaggcaatt gatatcgata agctgcgata atagtggaat cgcagtttat cagagataat
     6241 gaatcgctta ttc
//
LOCUS       DAJTYD010000192         6211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTYD010000192 DAJTYD010000000
VERSION     DAJTYD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.194"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            155..463
                     /locus_tag="OO585_003532"
     CDS             155..463
                     /locus_tag="OO585_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6630989.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(522..1616)
                     /gene="yaiW"
                     /locus_tag="OO585_003533"
     CDS             complement(522..1616)
                     /gene="yaiW"
                     /locus_tag="OO585_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6630990.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(1629..2849)
                     /gene="sbmA"
                     /locus_tag="OO585_003534"
     CDS             complement(1629..2849)
                     /gene="sbmA"
                     /locus_tag="OO585_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6630991.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            3201..4358
                     /gene="ampH"
                     /locus_tag="OO585_003535"
     CDS             3201..4358
                     /gene="ampH"
                     /locus_tag="OO585_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6630992.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(4359..4982)
                     /gene="iprA"
                     /locus_tag="OO585_003536"
     CDS             complement(4359..4982)
                     /gene="iprA"
                     /locus_tag="OO585_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6630993.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(5070..>6211)
                     /locus_tag="OO585_003537"
     CDS             complement(5070..>6211)
                     /locus_tag="OO585_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6630994.1"
                     /translation="ELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDA
                     NTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQD
                     TVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAK
                     WIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSAT
                     MSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKV
                     DGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEG
                     SAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         1548 a         1607 c         1502 g         1554 t
ORIGIN      
        1 attttgtgac tcacaacgaa gattcacgct tcgggaatat tcccaacatc aatttaccat
       61 gattctgata ttgacaatct aaatgacctg tcagacttgc cataagccgc tggtatcaca
      121 ccagaagaga agcagatgac ttccggaggt ttttatggct gatttcaccc tgtcaaaatc
      181 gctgtttagc ggaaaatatc gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt
      241 gtttgtgctg ttttgctttt gggctggggc gcaattgctg aacctgttag tgcatgcgcc
      301 cggcgtctat gagcgtttaa tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg
      361 tttaggtgtc ggcaccattt tcgggctgat cccgttttta gtaggctgcc tcatttttgc
      421 agtggtggcg ctatggctgc actggcgaca tcgccgccag taagatttac gttgccagat
      481 acggcctaaa acgccttatc tggcatgaca ttcctgtttg atcactgttt catacaacgc
      541 gcccgccgtt cgtctacgcg cttcgcaaac caggccgtag tcaggttgcg ggtgattttc
      601 gggctttcca gttgaatgcc aggcaacatt tcgcggggta gtgatttacc cgttttcgct
      661 tcggcaagtt gataaacttt cttgtacagc gccgtctcct caaaagagaa gctgtcacct
      721 ttctctaact gacggcgaat ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc
      781 gttgccagtt cggttttccc gggttcttta ctgtcgtaac gaattaaatc accgtccagt
      841 gccagcttca cgccgctggc cttactgacc gcgttctgaa acgcggcatt acggctggcg
      901 taccagccag cgttaaaatc agcaaagcgg tatatcggtg cgctatagct ggcgggataa
      961 ttcagtaaat ggtaagtacc aaaccacaac ccgccgcggc gactgaagac ttcctgacga
     1021 actgtaccgt ccattttcca cggataccct ttggtatgct gttcggcaaa agcaatgctg
     1081 acctgcatcg gcccaccggt gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt
     1141 ggtaccatgc tgatcagatc gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa
     1201 tctaaccgtt cgctataact tttgccgttt ggcgatttga tttttagcgc ggtatgaacc
     1261 agaaaagcag gaatgtgcat ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg
     1321 cttaagcccg gaacggccgg atctgcctga taatttgact cctgctgcgc caccgccagc
     1381 accgagcaga cgttttccag cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga
     1441 tctttcgccc aggcatcgcg gtctttcacg ctcgcaggca tcttctgccg cacgacgctc
     1501 gctacatcta tcgctttttc gccctttttc aacggttgcg gagcctgact actacacccc
     1561 gctaaaacca gaaccgccaa caatgacagc gaagatagat gattgacgcg cgacattact
     1621 tctccttttt agctcaaggt atgggttact tcctgaattt tgtcaccatc cagctcatgt
     1681 tcaaagctgc gcagacgttt gtagatagac atcaactcaa ccagtgtggt ccatgagtta
     1741 atcaggtact ggaaagcacc gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg
     1801 ccgagcgtaa tcgtaccggc aacaattgac ggaaacagca agaacaaacc gaaaacgtta
     1861 tcgacctgca aatagagaat gcgggcgatg ttgaaataca tatagtgaaa atagaggcga
     1921 aaatagtttt tccgtacggc gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg
     1981 gcatcgtctt caccataaac cagctcttta cggtaggcgg cctctactcg ctggttttta
     2041 aactccagcc ccggcagttt gatccctacc actgccagca atccggtccc catcagcgac
     2101 cagacgatag cggcaatcac cagaccatac ggaatgtgcc cgataatagg cagctccggc
     2161 acatgcgcgg agagcgttac cagcaccggc aggaaggcga ccaacgtcat gatggcgttg
     2221 ataaaactga cgcccatatt ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca
     2281 cgctgtgcgg caccttcgat atgacgcagt tgttgccagt tcgccatgta atattcgttc
     2341 atcgccgtac gccagcggaa cacatagtga ctgacaaaga agttgttcag tacactgatc
     2401 accaccgcga tcagcgcaat ccccagaaag acgcccactt cgcggtaaaa ttgttcgata
     2461 gtgactttat gcggcgaact tagcgcggtt tgaatcagat catagaacgg tgcataccag
     2521 gcgttgacgg cgactccgac ttccaccaaa aaccaggtga cgaagatgat cagtgcagta
     2581 ccgagtattg accagtattg ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa
     2641 agtcctacgc aaacaatgta gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc
     2701 gcggcgctaa tcgggatctg cccggaagcg ccggtgatac gcgccaccca gtctccccca
     2761 ccggcttgcc agaagataac ggcaatcaat gcccaaacaa aggccgagag aaaaaacgct
     2821 cccggctttg ggaaaaaaga cttaaacata tgcactcctg ctaacttctt atcgttttaa
     2881 caactgctgt gtattaaccg catgaccact acaaagcgtc agccaaatga aaccgcccat
     2941 tgtgacccat agcaaggcgc ttagttcgcc agcctgacca taagaattgt ttcgacggat
     3001 ttcacaaacc aaagattgtt tacataagcg gattaacacg cgcaggatca caaatgcagg
     3061 cattggtgag caggaaaaag atcgtgttta cgcgccgtta cgtttttaac actattattg
     3121 cactaattcg ccctttgcaa tccatcaatg agtagtataa atacgctcag ttaccttcat
     3181 tcaatctatg gacaccaccg ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg
     3241 cgtcattgac ctccgtccat gcggcgcagc cgatcactga accggagttt gcctctgata
     3301 ttgtcgatcg ttatgccgat catatttttt acggcagcgg tgccacgggg atggcgctgg
     3361 ttgttatcga cggtaaccag cgcgtctttc gcagttatgg cgaaacgcga cctggtaata
     3421 acgttcgccc gcagctggat tcggtcgtgc gtattgcttc cctcaccaag ctaatgacca
     3481 gtgaaatgct ggtgaaattg ctcgaccagg ggaccgtgaa gttaaacgat ccgctaagta
     3541 aatatgctcc gccaggcgca cgcgtgccaa cctacaatgg gacgcccatt accctagtga
     3601 atctggcaac ccataccagc gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc
     3661 cggtgtttgt ctggccaacg cgcgagcaac gctggaaata cctttctacg gcgaagctga
     3721 aagccgcgcc aggcagtcag gcggcgtact ctaaccttgc gttcgacctg ctggcagatg
     3781 cactggcgaa tgcgtccggc aagccttata cccagttgtt tgaagagcag attacccgtc
     3841 cattggggat gaaagacacc acctacaccc cctcaccgga tcagtgccgc cgtttaatgg
     3901 ttgctgagcg cggtgccagt ccgtgcaata acacgctggc ggcaattggc agcggcggcg
     3961 tctattccac gcctggcgat atgatgcgct ggatgcagca gtatttatca tctgattttt
     4021 atcagcgcag taaccaggca gatcgcatgc agacgttgat ttatcagcgc gcgcagttta
     4081 ccaaagtgat tggcatggat gtccccggca aagcggatgc gcttggttta ggctgggtat
     4141 atatggcccc gaaagagggt cgtccgggga ttattcagaa gacaggcggt ggtggcggtt
     4201 tcattaccta tatggcgatg atcccgcaga agaatattgg cgcgtttgtg gtggtcactc
     4261 gttcgccgtt gacgcgcttt aaaaatatga gcgatggcat caatgatctg gtgaccgagc
     4321 taagcgggaa taaaccgttg gttatccctg catcctgatt aatactctga aggcaaacgg
     4381 ttgatagcga ccagtttgcc tttattcatt tcgatatagc cgcctttacg caaagcagcg
     4441 agaacttctg cgacgacaga acgcgatatg cgtgtacgtt gatggatata attcatcaca
     4501 ccaatacgtg atcgcaattc ttcattccag tcaatcattg ataataatgt tgcgcggatt
     4561 tgttcgtagg aattatgccc aatgagctgc acgtcgcgta attccagaat tctattttgc
     4621 caggctaacc agtaaaaagc gtctcgccag agttgatttt gttcaataag cgtaatggtt
     4681 tgtttggctg gtagatgata tcccgtacaa tttccttctg atattaattt gtatggtata
     4741 tcgtttttca ttaaaccatc agccagcccc ataatataag aagcctgggt aataccgata
     4801 agtacgtttt cttctctacg cagagagata acgccctcta aaataaagaa agtatcttcg
     4861 ctattgacat cactggaaaa tataacttgc ttttcattat taaactcgaa gcgcgtaccg
     4921 tatctggaca aacatttatc gagcttacca aattcctgaa gaggtttaac tacagataac
     4981 atttgcgcgt cctttgcagt aatgcccgtc aaatccttga cgggcattat ttagattaaa
     5041 ttaccagaat ttcttcggag tgaagaatat taccaagtat atttaacacc cacgttcgcg
     5101 gaccagtctt gatctacgtc accaccaccg aggtagttag catcggtata ggcgctgaag
     5161 ttcttggtga agctgaactg agtacccaga ccaacacgta ccgcagaccc ttcagtaccg
     5221 ttatcgatgg aatcaccgtt cacatcgtta tcgttgtttg agtcgtcgta gacgtaagcc
     5281 agtttgaagt acggagtcag agcctgatct tcgctgtagg tgaaggtata acctgcatct
     5341 acacccagtt cataacgcat gctgtcgtaa gactgaccgt caactttcat gtcgttgctc
     5401 agctggtagt catcaccaga ctggaacaga ccagaaacgc tgccgtaagg agtctcgtaa
     5461 ccagcatcac ccagtttgaa gtcgtaaccg gctttcaaac cgaagcccca cgcgtcagag
     5521 ttggtgctac cgtcaacgta agtaccgttg ctcatggttg cagacaggtc gttgttgaag
     5581 tgagagtagc tcaagctacc atcaacaaag acgttgttcg cgaagtgagc agaagagtag
     5641 atgtaggcag tctggctgtc ttgatccacc tgaccagaac ggtcattcat gtcaccttta
     5701 gcgaagcctg cagccgcacc gacgatccac ttagcgttgt taccgtcaat tttggtatca
     5761 acaccgacca tgatgccgtt aacatcctga tcatagttga tggtgccgtt gtcgccgttg
     5821 aagttaccac cgaagtagct tacccatgcg ccgccgttat cagccaggcc atgacgagag
     5881 ttggtcagac gagtaccaac ggtgtcttgt tccaggttcc agatattggt gttcgcagac
     5941 gggatgctca gcgccatgtt agcgtagtcg gtcagctcca tctgttgcag aacaacggtg
     6001 ttaccgcgct gttcagcctg ataggtgtat gcacccaggt cagctttgtt agcagcagag
     6061 aaggtcgcgt tggtgttagc gtcgtttacc cgaataagtt ctttatcttt gtaatcagcg
     6121 atagtgccag cgccagttgc gttgtcgata cgaactttgt agttaccggt tgcagcaaca
     6181 acgttatcaa cagagctgtt atccagttca g
//
LOCUS       DAJTYD010000193         6050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTYD010000193 DAJTYD010000000
VERSION     DAJTYD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.195"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1900
                     /gene="dnaK"
                     /locus_tag="OO585_003538"
     CDS             <1..1900
                     /gene="dnaK"
                     /locus_tag="OO585_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6630995.1"
                     /translation="IDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLV
                     GQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQ
                     KMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVK
                     RIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGD
                     THLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVN
                     LPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILV
                     GGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLS
                     LGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQ
                     FNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQK
                     MVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTAL
                     ETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAE
                     FEEVKDKK"
     gene            1989..3119
                     /gene="dnaJ"
                     /locus_tag="OO585_003539"
     CDS             1989..3119
                     /gene="dnaJ"
                     /locus_tag="OO585_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6630996.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3223..3375)
                     /gene="mokC"
                     /locus_tag="OO585_003540"
     CDS             complement(3223..3375)
                     /gene="mokC"
                     /locus_tag="OO585_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6630997.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            3961..5127
                     /gene="nhaA"
                     /locus_tag="OO585_003541"
     CDS             3961..5127
                     /gene="nhaA"
                     /locus_tag="OO585_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6630998.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLCPSV"
     gene            5193..>6050
                     /gene="nhaR"
                     /locus_tag="OO585_003542"
     CDS             5193..>6050
                     /gene="nhaR"
                     /locus_tag="OO585_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6630999.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRIC"
BASE COUNT         1512 a         1446 c         1611 g         1481 t
ORIGIN      
        1 tatcgacctg ggtactacca actcttgtgt agcgattatg gatggcacca ctcctcgcgt
       61 gctggagaac gccgaaggcg atcgcaccac gccttctatt attgcctata cccaggatgg
      121 tgaaactctg gttggtcagc cggctaaacg tcaggcagtg acgaacccgc aaaacaccct
      181 gtttgcgatt aaacgcctga ttggccgccg cttccaggac gaagaagtac agcgtgatgt
      241 ttccatcatg ccgttcaaaa ttattgctgc tgataacggc gacgcatggg tcgaagttaa
      301 aggccagaaa atggcaccgc cgcagatttc tgctgaagtg ctgaaaaaaa tgaagaaaac
      361 cgctgaagat tacttgggtg aaccggtaac tgaagctgtt atcaccgtac cggcatactt
      421 taacgatgct cagcgtcagg caaccaaaga cgcaggccgt atcgctggtc tggaagtaaa
      481 acgtatcatc aacgaaccga ccgcagctgc gctggcttac ggtctggaca aaggtactgg
      541 caaccgtact atcgcggttt atgacctggg gggtggtact ttcgatattt ctattatcga
      601 aatcgacgaa gttgatggcg aaaaaacctt cgaagttctg gctaccaacg gtgataccca
      661 cctgggtggt gaagacttcg acagccgtct gatcaactac ctggttgaag aattcaagaa
      721 agatcagggc attgacctgc gcaacgatcc gctggcaatg cagcgcctga aagaagcggc
      781 agaaaaagcg aaaatcgaac tgtcttccgc tcagcagacc gacgttaacc tgccgtacat
      841 cactgcagat gcgaccggtc cgaaacacat gaacatcaaa gtgactcgtg cgaaactgga
      901 aagcttggtt gaagatctgg taaaccgttc cattgagccg ctgaaagttg cactgcagga
      961 cgctggcctg tccgtatctg atatcgacga cgttatcctc gttggtggtc agactcgtat
     1021 gccaatggtt cagaagaaag ttgctgagtt ctttggtaaa gagccgcgta aagacgttaa
     1081 cccggacgaa gctgtagcaa tcggtgctgc tgttcagggt ggtgttctga ctggtgacgt
     1141 gaaagacgta ctgctgctgg acgttacccc gctgtctctg ggtatcgaaa ccatgggcgg
     1201 tgtgatgacg acgctgatcg cgaaaaacac cactatcccg accaagcaca gccaggtgtt
     1261 ctctaccgct gaagacaacc agtctgcggt aaccatccat gtgctgcagg gtgaacgtaa
     1321 acgtgcggct gataacaaat ctctgggtca gttcaacctg gatggtatca acccggcacc
     1381 gcgcggcatg ccgcagatcg aagttacctt cgatatcgat gctgacggta tcctgcacgt
     1441 ttccgcgaaa gataaaaaca gcggtaaaga gcagaagatc accatcaagg cttcttctgg
     1501 tctgaacgaa gatgaaatcc agaaaatggt acgcgacgca gaagctaacg ccgaagctga
     1561 ccgtaagttt gaagagctgg tacagactcg caaccagggc gaccatctgc tgcacagcac
     1621 ccgtaagcag gttgaagaag caggcgacaa actgccggct gacgacaaaa ctgctatcga
     1681 gtctgcactg actgcactgg aaactgctct gaaaggtgaa gacaaagccg ctatcgaagc
     1741 gaaaatgcag gagctggcac aggtttccca gaaactgatg gaaatcgccc agcagcaaca
     1801 tgcccagcag cagactgccg gtgctgatgc ttctgcaaac aacgcgaaag atgacgatgt
     1861 tgtcgacgct gaatttgaag aagtcaaaga caaaaaataa tcgccctata aacgggtaat
     1921 tatactgaca cgggcgaagg ggaatttcct ctccgcccgt gcattcatct aggggcaatt
     1981 taaaaaagat ggctaagcaa gattattacg agattttagg cgtttccaaa acagcggaag
     2041 agcgtgaaat caaaaaggcc tacaaacgcc tggccatgaa ataccacccg gaccgtaacc
     2101 agggtgacaa agaggccgag gcgaaattta aagagatcaa ggaagcttat gaagttctga
     2161 ccgactcgca aaaacgtgcg gcatacgatc agtatggtca tgctgcgttt gagcaaggcg
     2221 gcatgggcgg cggcggtttt ggcggcggcg cagacttcag cgatattttt ggtgacgttt
     2281 tcggcgatat ttttggcggc ggacgtggtc gtcaacgtgc ggcgcgcggt gctgatttac
     2341 gctataacat ggagctcacc ctcgaagaag ctgtacgtgg cgtgaccaaa gagatccgca
     2401 ttccgactct ggaagagtgt gacgtttgcc acggtagcgg tgcaaaacca ggtacacagc
     2461 cgcagacctg tccgacctgt catggttctg gtcaggtgca gatgcgtcag ggtttctttg
     2521 ccgtgcagca gacctgtcca cactgtcagg gccgcggtac gctgatcaaa gatccgtgca
     2581 acaaatgtca tggtcatggt cgtgttgagc gcagcaaaac gctgtccgtt aaaatcccgg
     2641 caggggtgga cactggagac cgcatccgtc ttgcgggcga aggtgaagcg ggccagcatg
     2701 gcgcaccggc aggcgatctg tacgttcagg ttcaggttaa acagcacccg attttcgagc
     2761 gtgaaggcaa caacctgtat tgcgaagtcc cgatcaactt cgctatggcg gcgctgggtg
     2821 gcgaaatcga agtaccgacc cttgatggtc gcgtcaaact gaaagtgcct ggcgaaaccc
     2881 agaccggtaa gcttttccgt atgcgcggta aaggcgtcaa gtctgtccgc ggtggcgcac
     2941 agggtgattt gctgtgccgc gttgtcgtcg aaacaccggt aggcctgaac gagaagcaga
     3001 aacagctgct gcaagagctg caagaaagct tcggtggccc aaccggcgag cacaacagcc
     3061 cgcgctcaaa gagcttcttt gatggtgtga agaagttttt tgacgacctg acccgctaac
     3121 ctccccaaaa gcctgcccgt gggcaggcct gggtaaaaat agggtgcgtt gaagatatgc
     3181 gagcacctgt aaagtggcgg ggatcactcc ccgccgttgc tcttactcgg attcgtaagc
     3241 cgtgaaaaca gcaacctccg tctggccagt tcggatgtga acctcacaga ggtcttttct
     3301 cgttaccagc gccgccacta cggcggtgat acagatgacg atcagggcga caatcatcgc
     3361 cttatgctgc ttcattgctc tcttctcctt gaccttacgg tcagtaagag gcactctaca
     3421 tgtgttcagc atataggggg cctcgggttg atggtaaaat atcactcggg gcttttctct
     3481 atctgccgtt cagctaatgc ctgagacaga cagcctcaag cacccgccgc tattatatcg
     3541 ctctctttaa cccattttgt tttatcgatt ctaatcctga agacgcctcg catttttgtg
     3601 gcgtaatttt ttaatgattt aattatttaa ctttaattta tctctttatc gcaattattg
     3661 acgacaagct ggattatttt tgaaatattg tcctaacaaa taacgccgac tgacaagaaa
     3721 ttaattatta cttttcctaa ttaatccctc agaaatcctc accttaagct atgattatct
     3781 gagcttaggg tcactcgtca gcgcttacag ccgtcaaaaa ctcatctcac cgctgatggc
     3841 gaaaattcta caaaggctcg taaaaaacga attattccta cactataatc tgattttaac
     3901 gatgattcgt gcggggtaaa atagtaaaaa cgatttattc acctgaaaga gaaataaaaa
     3961 gtgaaacatc tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt
     4021 gccgctatcc tggcgatgat gatggccaac agcggcgcaa ccagtggatg gtatcacgac
     4081 tttctggaga cgccggttca gctccgggtt ggttcactcg aaatcaacaa aaacatgctg
     4141 ttatggataa atgacgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa
     4201 cgtgaactga tgcaaggatc gctagccagc ttacgccagg ccgcatttcc agttatcgcc
     4261 gctattggtg ggatgattgt gccggcatta ctctatctgg cctttaacta tgccgatccg
     4321 attacccgcg aagggtgggc gatcccggcg gctactgaca ttgcctttgc acttggagtg
     4381 ctggcgctgt tgggaagtcg tgttccgtta gcactgaaga tctttttgat ggctctggct
     4441 attatcgacg atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg
     4501 atggcctctc ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt
     4561 ggtgtacgcc gcacgggcgt ctatattctg gttggcgtag tgttgtggac tgcggtgttg
     4621 aaatcggggg ttcacgcaac cttggcgggg gtaattgtcg gcttctttat tcctttgaaa
     4681 gagaagcatg ggcgttctcc agcgaagcga ctggagcatg tgttacaccc gtgggtggcg
     4741 tatctgattt tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggcgtcacg
     4801 ctggatggct tgacctccat tctgccattg gggatcatcg ctggcttgct gattggcaaa
     4861 ccgctgggga ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct
     4921 gagggaacga cttatcagca aattatggcg gtggggatcc tgtgcggtat cggttttact
     4981 atgtctatct ttattgccag cctggccttt ggtagcgtag atccagaact gattaactgg
     5041 gcgaaactcg gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta
     5101 cgcgttcgtt tgtgtccatc agtgtgacag gacggtttac cggggagcca taaacggctc
     5161 ccttttcatt gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt
     5221 attacttctg gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt
     5281 taactccaca aaccattacc ggacagatcc gggctctgga agagcgcctg caaggcaaat
     5341 tatttaagcg caagggacgt ggtctcgaac ccagcgagct gggagaactg gtctatcgct
     5401 atgccgataa aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag
     5461 aatccaattt attgtttgac gttggcgtgg ctgatgcact ttccaaacgc ctggtcagta
     5521 gcgtgctgaa tgctgcagtg gtagaaggcg agccgattca tcttcgctgc ttcgaatcca
     5581 cccacgaaat gctactggag caattaagcc agcataaact ggatatgatc atttccgact
     5641 gtccgataga ctctacgcag caggaaggcc tgttctccgt gagaattggc gaatgtggcg
     5701 tgagtttctg gtgtacaaat ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac
     5761 ggcgactttt gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta
     5821 actcccaggg attaaacgta gaaatcctcg gcgagtttga tgatgccgcg ttgatgaaag
     5881 cttttggtgc aatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt
     5941 atgccgataa aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg
     6001 ctatttttgc tgagcggatg attcagcacc cggcggtaca gcgaatctgc
//
LOCUS       DAJTYD010000194         6048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTYD010000194 DAJTYD010000000
VERSION     DAJTYD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.196"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1262)
                     /gene="tcyP"
                     /locus_tag="OO585_003543"
     CDS             complement(<1..1262)
                     /gene="tcyP"
                     /locus_tag="OO585_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6631000.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPL"
     gene            complement(1407..1985)
                     /locus_tag="OO585_003544"
     CDS             complement(1407..1985)
                     /locus_tag="OO585_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6631001.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKH"
     gene            complement(2148..2816)
                     /gene="hxpB"
                     /locus_tag="OO585_003545"
     CDS             complement(2148..2816)
                     /gene="hxpB"
                     /locus_tag="OO585_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6631002.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            2963..3499
                     /locus_tag="OO585_003546"
     CDS             2963..3499
                     /locus_tag="OO585_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6631003.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3540..4400)
                     /locus_tag="OO585_003547"
     CDS             complement(3540..4400)
                     /locus_tag="OO585_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6631004.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4506..4796)
                     /gene="ghoS"
                     /locus_tag="OO585_003548"
     CDS             complement(4506..4796)
                     /gene="ghoS"
                     /locus_tag="OO585_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6631005.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(4897..5826)
                     /gene="pfkB"
                     /locus_tag="OO585_003549"
     CDS             complement(4897..5826)
                     /gene="pfkB"
                     /locus_tag="OO585_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6631006.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1579 a         1509 c         1471 g         1489 t
ORIGIN      
        1 ataagcggtt caacggagat taacagcgcc accagggtta ctggcaggcc catcgcaggc
       61 agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc tgcggaacta
      121 acggtaacaa taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt aatgccaacc
      181 gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc gttctgacca
      241 atggttgcac cgaaagaggc ggcgaaactg gcgatggatt caggaacgcc cagacgacgc
      301 gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt aaaggcaaac
      361 gtcagcacag gccatacctt acggaagtac ttcagcggac tcacgccatt aatgcccagc
      421 agaatgccat gcactgcaaa cataatcagc agaccgaggt aggacgcgac aacgaaactt
      481 cccagtttga tgatgtcttg caagttagaa cctgcaacca ctttggtcat tagagccaga
      541 acgccgtaag gggtcaactg catgacaagg cgaaccagtt tcatcaccca gctttgtagg
      601 gtatcgatag cggctaagac gcgttcacct ttcggcgcat catccttcag cagtttcaat
      661 gcagctacgc cgaggaatgc ggcaaaaatt accacgctga taattgacgt cggattggct
      721 ccggtaagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg cggaacgctc
      781 agatcagaga ctttaccaac atagttactt tcaatggcgt tcagacgtgc agtttctgca
      841 ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag cacaccgacc
      901 agcgccgcaa tcagcgtggt aaacaaaagc gtaccgatgg tcagaaaact gattttgcct
      961 aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa gactaacggc
     1021 ataacgatca tttgcagcag ttgaacatag ccgttaccaa cgatgttaaa ccactgtaca
     1081 gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag gccaaaaacc
     1141 acacccatca ccagacccac cagcactttt ttcgccagac tccactgttt atggcgggtc
     1201 tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa tggaaagttc
     1261 atccccgttc tcctgatctt attatttcta tcactgcgta gcagtcatag tgttggcgca
     1321 agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat tccagaaagg
     1381 tgatttttgt ctttttagta ttcaacctaa tgcttaataa gccgatgaaa ctggatctgc
     1441 aaggtattga ttatttgcga tgaccaacga acagcgctgt tctcgggtaa tgaatggggc
     1501 atccataccg accagacaaa taatgccata cagataaaac gttccagttg gttgcccttt
     1561 tggggaacta ggataggcaa gcggaaacgc cagcggcatg gccagagcag gggaacaccg
     1621 gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac ccagcaccat tccttgtagc
     1681 gcatcagccg gaatgaacca gccttccgga accttaaggt aaaaggttgc cagcagcgca
     1741 aataccgcca gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc gatcggtttt
     1801 gatatccatt ttaatcgctg cccaagaaac gactttgggt gatcgatgtc cggtaacaaa
     1861 cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag cacgggcgtc
     1921 agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg accttccgcc
     1981 gtcatgataa aactcactaa actgtcgata cgtacagtat aggattttta tacagtgcac
     2041 ggaagtggtg acggtgtaac tctttctctt aaattgacga ttaatccgtc agttgaatgc
     2101 aaattcagat aaagaaccag gggcagcgtg atcactgccc ctggtcatta accgagaagg
     2161 tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac aaaacgtgga
     2221 tcattttgcg cttctggcgc aggaacgacg atggaacgca tgcgggctgc tttagaagcg
     2281 atcatgccat ttaccgagtc ttccagcgcc acgcaggtca gtgggtcaac gcccagtttt
     2341 gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt aaggcagttt ttcagccgag
     2401 gcgagggcat cgaaactgtc gcgtaagtca aacatggtca acactttttc cagcatatgt
     2461 agtggtgacg cggaggccag tcccaccaat aaaccttgtt ctttgcataa cgcaacagct
     2521 tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc acgggcaata
     2581 acccgttcta ctacttcctg acggcttggc ccattccacg gttgccgggc gtaccaaaga
     2641 tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg acgggagata
     2701 tccaccccca ggcttgccat cacatccagt tcggctcgat cccataaagg ttctgagtcg
     2761 ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt tgacattaca
     2821 acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt aagcgcctat
     2881 catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa gtgaaacgat
     2941 gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacacattg ctgttttgaa
     3001 aaggattttg ggttgggtga tttttatacc tgcattgatc tctacattga tttctttgct
     3061 gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca tgctcgactt
     3121 tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc tgttctggta
     3181 taactccccg acgcccaatt ttaatggtgg cgtgaacgtt atgttctggg tgattttcat
     3241 cctgattttt gtcggactgg cgttgcagga ttccggtgcc cgaatgagtc gccaggcgcg
     3301 cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag gggaagaggg
     3361 gctaacgcgt gaacaaattg agtcccgtat tgttgttccg caccatacta ttttcctgca
     3421 gtttttctca ctgtatatcc tgccggttat ttgtattgct gcaggttatg tgttcttttc
     3481 tctgcttggg tttatttaat caaaaggccg cacagagcgg ccattatctt caacccatat
     3541 catgctgcta ataatctatc cagtgactgc tgagcaataa ccaaatgctg accgccaaat
     3601 aaccttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc aaggaaatcg
     3661 gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg ttcagtatgc
     3721 agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggcagg gtcgaaaatg
     3781 tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg caacagagaa
     3841 ggctgcggtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc gtcgatattg
     3901 ccgaaagcga tccctttctc tgctgccagt tccaactgcc agccaatccg ttgttcagca
     3961 aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga gagcgagtta
     4021 tcaaaatcga ggccaaattg tggttggtca ctccattgat gtaaacgcgc aatttgctga
     4081 ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc catcaccaga
     4141 aaactgtagt cacggtcagc gcctactgcc caaaccttag gcacggtgac ggttttacta
     4201 cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag ttccctttca
     4261 tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc atggacttct
     4321 ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc gctcaaaaga
     4381 cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca taaggttagc
     4441 gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta ttgcccaaaa
     4501 accatttatt ttctgttgct attccattcc tcccaggtcg tgatggtgat ttcaggatct
     4561 ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc ttcactttgg
     4621 gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc atcatccgtc
     4681 atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat ttcagtcaat
     4741 gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact ggccatagtt
     4801 cacccctttg ttatcacatg acttttgagc atagtcggag aaacgcgttg ccgacaggtt
     4861 ggtggtgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt aagcgtaaat
     4921 tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg ctgcactccc
     4981 cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca gtttcagtgt
     5041 catcgcgccg accatgctgt caccagcgcc aacggtactc tggcttttca ccggtggtgg
     5101 caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac caagggaaac
     5161 gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc ggacatcgtc
     5221 cggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag gcttaaccaa
     5281 ctcgatgtta ccaattgcca gtgctgcact taacgcttcg ccagaactgt cgacgatgca
     5341 gcggatccct tgtttttgcg aagcggaaat cagttgggtt aatttttcca gcttcacacc
     5401 tggcggcagg cttccgctta tgactaggat ggccccggat tcaatttcca gaacttgctc
     5461 ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa aacgatactg
     5521 ctcaccgctt gcttccacat gtacatgtaa attctgccgg gtccagtctt tggcttctac
     5581 agtagcgacg gggacatttt catccgccaa cagtgaaacc aggtgttcgc cggtcgcgcc
     5641 acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc gggcgacgtt
     5701 gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc cttcgggata
     5761 aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg tatagatacg
     5821 taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcg gaaagtgaag
     5881 aaattaacaa tatgatttat tggagcttta tttaaaattt tgcagataaa tatatataaa
     5941 taaaaatctg ttttcgccac atgcttattc aaaaaatgaa ataagaatac attgctaagt
     6001 tattcgcgcc tttttataat tcgttacctt tccagggacg ctaatctt
//
LOCUS       DAJTYD010000195         6004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTYD010000195 DAJTYD010000000
VERSION     DAJTYD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.197"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            211..1464
                     /gene="avtA"
                     /locus_tag="OO585_003550"
     CDS             211..1464
                     /gene="avtA"
                     /locus_tag="OO585_003550"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6631007.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(1615..2088)
                     /locus_tag="OO585_003551"
     CDS             complement(1615..2088)
                     /locus_tag="OO585_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6631008.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(2190..3038)
                     /gene="yiaJ"
                     /locus_tag="OO585_003552"
     CDS             complement(2190..3038)
                     /gene="yiaJ"
                     /locus_tag="OO585_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6631009.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            3239..4237
                     /gene="yiaK"
                     /locus_tag="OO585_003553"
     CDS             3239..4237
                     /gene="yiaK"
                     /locus_tag="OO585_003553"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6631010.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            4249..4717
                     /locus_tag="OO585_003554"
                     /pseudo
     CDS             4249..4717
                     /locus_tag="OO585_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            4835..5308
                     /gene="yiaM"
                     /locus_tag="OO585_003555"
     CDS             4835..5308
                     /gene="yiaM"
                     /locus_tag="OO585_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCR6631011.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            5311..>6004
                     /locus_tag="OO585_003556"
     CDS             5311..>6004
                     /locus_tag="OO585_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6631012.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFR"
BASE COUNT         1443 a         1540 c         1503 g         1518 t
ORIGIN      
        1 aaagtgctgg tcatctgggc tgggcaacag tgacttttcc ggcttcccgt tcatcagtac
       61 ctcgggaagc cgccaaccag gataaaatgt cagccctaat cagcgttgca ggataaagca
      121 ccgctcactc ttcaacagac cgatttgcac cccagcaaat gtagcgttat tgttaccttc
      181 cttgctacag agttcgacag atatcccgct atgacattct ccctttttgg tgacaaattt
      241 acccgccact ccggcattac gctgctgatg gaagatctga acgacggttt acgcacgcct
      301 ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc cggaaatgca ggactacttc
      361 cagacgctac tgaccgacat gctggaaagt ggcaaagcga ctgatgcact gtgtaactac
      421 gacggtccac aggggaaaac ggagctactc acactgcttg ccggaatgct gcgcgagaag
      481 ttgggttggg atatcgaacc acagaatatt gcactaacaa acggcagcca gagcgcgttt
      541 ttctacttat ttaacctgtt tgccggacgc cgtgccgatg gtcgggtcaa aaaagtgctg
      601 ttcccgctgg caccggaata cattggctac gctgacgccg gactggaaga agatctgttt
      661 gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc agtttaaata ccacgtcgat
      721 tttgagcatc tgcatattgg cgaagaaacc gggatgattt gcgtctcccg gccgacgaat
      781 ccaacaggca atgtgattac tgacgaagag ttgctgaagc ttgacgcgct ggcgaatcaa
      841 cacggcattc cgctggtgat tgataacgct tatggcgtcc cgttcccggg tatcatcttc
      901 agtgaagcgc gcccgctatg gaatccgaat atcgtgctgt gcatgagtct ttccaagctg
      961 gggctgcctg gctcccgctg cggcattatc atcgccaatg aaaaaatcat cactgccatc
     1021 accaatatga acggcattat cagcctggca cctggcggta ttggtccggc gatgatgtgt
     1081 gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa cagtcatcaa accgttttac
     1141 taccagcgtg ttcaggaaac tatcgccatc attcgccgct atttaccgga aaatcgctgc
     1201 ctgattcata aaccggaagg agccattttc ctctggctat ggtttaagga tttgcccatt
     1261 acgaccgagc agctctatca gcgcctgaaa gcacgcggcg tgctgatggt gccggggcac
     1321 aacgtcttcc cgggactgga taaaccgtgg ccgcatacgc atcaatgtat gcgcatgaac
     1381 tacgtaccag agccggagaa aattgaggcg ggggtgaaga ttctggcgga agagatagaa
     1441 agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc tacgcttatc aggcctacat
     1501 gatttctgca atatattgaa tttggaagaa tttgtaggcc ggataaggcg tttacgccgc
     1561 atctggcata aacaaagcgc actattccgg cgttgagaaa cgccggaaaa cgtttcaaac
     1621 aggctgctgc cgtagccgat gccgctgcac tttctcgaca tccatgcact gcaacgcctt
     1681 tgtcgggcag gcttcaacac aggccggccc cgtctcccga tgccagcaca gatcgcactt
     1741 aatcgccctc gcctttttgc gcgaagaaac gacctccatc gcaccaaacg ggcaagccag
     1801 catacagctt ttacagccaa tgcaacgtgt ttgttcaacg aaaatatgcc catgttcgcg
     1861 gcttatcgcg tcaacagggc agacattcgc gcacggtgca tcttcacact gatgacaggc
     1921 tactgccgtg gtccagctgt ggtctttaat gacacgaata cgggaaataa actcgtctgg
     1981 tgacaacgca gcgcaatcct gattctcctg atgcgacact gcgcaagcca cttcacaggt
     2041 acggcaaccg atacatttcg tcgcatccgc aataataaac cggttcatga tcactccttt
     2101 gcactaaaac agcagagtgc cagagagcgc gtccctactg ctttgatcca acgaaggaaa
     2161 tgttaaagtt ttggcacttg tccaaagcgt tatgtgatcg cgcccaggtc atccctgaca
     2221 gtaaatccca gttcattaga aatagcctgc gcggtttcac gcagtggttt caggagattt
     2281 ttctctccca cctgtttcag acgtgatgtc gaaagcgaaa tcgacacggc gtacggcacc
     2341 cgcccatgaa tatcaaacac cggaacagca atacaggaga cgccgagttc gttttcttct
     2401 ctgtccatcg ccgctccgct ttcacgaatg tgcgccagtt cgtcgaacat cgcgggcagc
     2461 tcggtaatgg tattgcgggt taacggctgg atctcatgct gatggttttc ccagtatgac
     2521 ttcacgtagt ccgggtgacc aaacgccatg tagatcttgc ccattgcgga acagtagagc
     2581 ggcatatgct ggccaatata ggcacgggtt cgcagcatcc cggttgtggg ttccagctta
     2641 taaatcaaaa tagcgtgatc gtcttcgcgg ctggagaagt taatggtttc accagtggcg
     2701 atgttcagtg cctcaagatg cggagcggcg atatgaatga tattcagcga agacagcgcc
     2761 ttctgcccga cggcaataaa tttggtagtc aggcggtaac tccctgcggc gggcgcggtg
     2821 gtcacatagc cgcaggactg taatccctgc aataagcgat ggacggtact cttatttaaa
     2881 ccagccagct ccgaaagatg cgccaacgga caaccgtttg gatagttgct caaaatctca
     2941 atcagcatca acccgcgaaa cagactctgg cttccggctg gacgctcttt ttcctgcgcc
     3001 atctcgttct ctttttttcc catcacttct ttccccattt tgtcgcgtcc tgatggtagc
     3061 gcaaagtgtg ccgtagttca cgatctcgac agataattta taaccacttg atttttatgt
     3121 cttttgaaat tcatcaatca gattgctttg ttaaaaagtg atcgatatat ttgaaatcaa
     3181 gtttcgcata ttgaaatttt aagccaaaaa agcgatcaaa aaaacaagga agcctgggat
     3241 gaaagtgaca tttgagcagt taaaagcagc ctttaatcgg gccttaattt cacgcggcgt
     3301 tgacagcgaa acggctgacg cctgtgcaga gatgttcgcc cgcaccaccg aatccggcgt
     3361 ttattctcac ggcgttaatc gtttccctcg tttcattcaa caactggaaa acggcgatat
     3421 cattcctgat gcccaaccca aacgtataac cagcctcggc gcaattgaac agtgggatgc
     3481 ccagcgttcg atcggcaacc tgacggcgaa aaagatgatg gatcgcgcca ttgaactggc
     3541 tgccgatcac ggtattggtc tggtggcact tcgtaatgcc aaccactgga tgcgcggcgg
     3601 cagctacggc tggcaggcgg cggaaaaagg ctatattggc atttgctgga ccaactccat
     3661 cgccgtaatg ccgccgtggg gcgcaaaaga gtgtcgcatt ggcactaacc cgctgatcgt
     3721 cgccattcct tccacgccga tcaccatggt cgatatgtcg atgtcgatgt tctcttacgg
     3781 catgttagaa gttaaccgcc tggcgggccg tcagctcccg gtcgatggtg gctttgatga
     3841 tgagggcaat ttgaccaaag aacctggcgt tatcgagaag aatcgccgca ttttgccgat
     3901 gggctactgg aaaggttctg gcatgtcgat tgtgctggat atgatcgcta ctctcctttc
     3961 cgacggcgca tcggttgccg aagtcaccca ggacaacagc gacgaatacg gcatttcaca
     4021 aatttttatt gccattgaag tggacaagct tatcgacggt cccacccgcg atgccaagct
     4081 gcaacgcatc atggattacg ttactaccgc tgaacgcgct gacgaaaacc aggccatccg
     4141 cttacccggc cacgaattta ctaccctgct ggccgaaaac cgccgtaacg gcatcactgt
     4201 tgatgacagc gtgtgggcca aaatccaggc gttatgagga gataagtcat gatttttgga
     4261 catatcgcgc agcccaatcc gtgccgtttg cccgccgcca ttgaaaaggc gctcgatttt
     4321 ctgcgcgcta ccaacttcaa cgtcctggag ccgggcgttg tcgaaatcga cggcaagaat
     4381 atttatgcgc aaattatcga tttaaccaca cgcgaagcgg tggaaaaccg tccggaagtg
     4441 catcgtcggt atatcgatat tcagtttctg gcgtggggtc aagagaaaat cggcattgct
     4501 attgatacag gaaataataa agtcagcgaa tcactattag agcagcgcga tattattttt
     4561 tatcatgtca gtgaacatga atcatttata gaaatgatac cgggcagcta cgctattttc
     4621 ttcccgcagg atgttcaccg accaggttgt attctgcaaa caggcctctg agatacgaaa
     4681 aattgtggtt aaagtcgcat taacagcgct gaattaatca aataatcgtc tacgccagaa
     4741 cgactgggta atgttattgc tctaactata ttaataattt aggttgctgc acatgcaact
     4801 tgaattatga cagacatagc ctcaaggaat agctatgaaa aaaatacttg aagtaatact
     4861 ggcgattaat ctcgccgtac tttcctgtat tgtgtttata aacattattt taagatatgg
     4921 ttttcagaca agcattttat ctgtagatga attgtcacgt tatttatttg tctggttaac
     4981 atttattggc gcgattgtgg cttttatgga taacgcccac gttcaggtca cctttctggt
     5041 ggagaaactc tcccccgcat ggcaacgacg agtcgctctg gttacccatt ccttaatctt
     5101 atttatttgt ggcgcactgg cctggggcgc aacactaaaa acaattcagg actggagcga
     5161 ttattcaccg atcctcggtt tgcccatcgg cctgatgtat gccgcttgtc tgcccaccag
     5221 cctcgttatc gcattctttg aactgcgtca tttatatcaa ctcatcacgc gtagcaattc
     5281 tctaacgtcg ccaccgcaag gagcttaatc atggctgtgc tgatttttct gggctgtctg
     5341 ttgggtggta ttgctatcgg tttgcctatt gcctgggcac tgttgttgtg cggggcggcg
     5401 ttaatgttct ggctggacat gtttgatgtc cagatcatgg cacaaacgct ggtgaacgga
     5461 gccgatagct tctccctgct ggcgattccg ttctttgtgc tggcgggtga aatcatgaat
     5521 gcgggcggct tgtcaaaacg cattgttgac ctgccgatga aactggtggg gcataaacca
     5581 ggcgggctgg gctacgtcgg cgtgctggca gctatgatta tggcgagcct ttctggttct
     5641 gccgttgcgg ataccgccgc cgttgccgct ctactggtgc cgatgatgcg cagcgccaac
     5701 tacccggtca accgggcggc ggggctgatt gcctctggcg gcattatcgc gccaattatt
     5761 cccccttcca ttccgtttat tatcttcggc gtttccagcg gattatccat cagtaagctg
     5821 tttatggcgg gcattgcccc tgacatgatg atgggcgcaa cgctgatgct tacctggtgg
     5881 tggcaggcca gtcgccttaa tttaccccgc cagcaaaaag caacgatgca ggaaatctgg
     5941 cactcttttg tctccggtat ctgggctctg tttcttccag tcatcattat tggcgggttt
     6001 cgct
//
LOCUS       DAJTYD010000196         5992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTYD010000196 DAJTYD010000000
VERSION     DAJTYD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.198"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(64..511)
                     /locus_tag="OO585_003557"
                     /pseudo
     CDS             complement(64..511)
                     /locus_tag="OO585_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
     gene            668..1597
                     /gene="metA"
                     /locus_tag="OO585_003558"
     CDS             668..1597
                     /gene="metA"
                     /locus_tag="OO585_003558"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6631013.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1866..3467
                     /gene="aceB"
                     /locus_tag="OO585_003559"
     CDS             1866..3467
                     /gene="aceB"
                     /locus_tag="OO585_003559"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR6631014.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            3497..4794
                     /gene="aceA"
                     /locus_tag="OO585_003560"
                     /pseudo
     CDS             3497..4794
                     /gene="aceA"
                     /locus_tag="OO585_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            5075..>5992
                     /locus_tag="OO585_003561"
     CDS             5075..>5992
                     /locus_tag="OO585_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR6631015.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQ"
BASE COUNT         1454 a         1590 c         1529 g         1419 t
ORIGIN      
        1 aaaaaatgcc gggtgcgacg ctggcgcgtc ttctccagcc tgggcaggca cggcaggggc
       61 ggcttattcc cccacgtacg ccagattcag caacggatac ggtttcccca aatcgtccac
      121 ctcagagcgt cccgtaacct taaaacccac cttcttatag aacccaaccg cctgctcatt
      181 ttgctcatta acgttggttg tcagttccgg tgccatcgag agcgcatgct ccaccagcat
      241 ccgacccacg ccgcagccgc acacatcagg atcgataaac agcgcatcca tatgctgccc
      301 acttaacaac ataaacccaa ccggctgatc ccgttcatta accgcgatcc acaacggcgc
      361 ttccggcagg aaggaacgaa ctagctagca cctccagctc ggcccgatac tctgctgata
      421 aaaaatcgtg tgtggcatcg acagatcgac accaaatcgc aacgagttcc tccccttcct
      481 catgccgtga gcggcgaata ctaataacca ttttctctcc ttttagtcat tcttatattc
      541 taacgtagtc ttttccttga aactttctca ccttcaacat gcaggctcga cattggcaaa
      601 ttttctggtt atcttcagct atctggatgt ctaaacgtat aagtgtatgt agtgaggtaa
      661 tcaggttatg ccgattcgtg tgccggacga gctacccgcc gtcaatttct tgcgtgaaga
      721 aaacgtcttt gtgatgacaa cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt
      781 tctgatcctt aacctgatgc cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct
      841 ttcaaactca cctttgcagg tcgatattca gctgttgcgc atcgattccc gtgaatcgcg
      901 caacacgccc gcagagcatc tgaacaactt ctactgtaac tttgaagata ttcaggaaca
      961 gaactttgac ggtctgattg taaccggtgc gccgctgggc ctggtggagt ttaatgatgt
     1021 cgcttactgg ccgcagatca aacaggtgct ggagtggtcg aaagatcacg tcacctcgac
     1081 gctgtttgtc tgctgggcgg tacaggccgc gctcaatatc ctctacggca ttcctaagca
     1141 aactcgcacc gacaaactct ctggcgttta cgagcatcat attctccatc ctcatgcgct
     1201 tctgacgcgt ggctttgatg attcattcct ggcaccacat tcgcgctatg ctgactttcc
     1261 ggcagcgttg attcgtgatt acaccgatct ggaaattctg gcagagacgg aagaagggga
     1321 tgcatatctg tttgccagca aagataagcg cattgccttt gtgacgggcc atcccgaata
     1381 tgatgcgcaa acgctggcgc aggaatattt ccgcgatgtg gaagccggac tagacccgga
     1441 agtaccgtat aactatttcc cgcacaatga tccgcaaaat acaccgcgag cgagctggcg
     1501 tagtcacggt aatttgctgt ttaccaactg gctcaactat tacgtctatc agatcacgcc
     1561 atacgatcta cggcacatga atccaacgct ggattaatct tctgtgatag tcgatcgtta
     1621 agcgattcag caccttacct caggcacctt cgggtgcctt ttttatttcc gaaacgcacc
     1681 tcaggaggtg aataaatttt attcacattg ttatcaataa gttatcaagc attttcaatt
     1741 aaaatggaaa ttgtttttga ttttgcattt taaatgagta gtcttagttg tgctgaacga
     1801 aaagcgcaca acgatccttc gttcacagtg gggaagtttt cggatccatg acgaggagct
     1861 gcacgatgac tgaacaggca acaacaaccg atgaactggc tttcacaagg ccgtatggcg
     1921 agcaggagaa gcaaattctt actgccgaag cggtagaatt tctgactgag ctggtgacgc
     1981 attttacgcc acaacgaaat aaacttctgg cagcgcgcat tcagcagcag caagatattg
     2041 ataacggaac gttgcctgat tttatttcgg aaacagcttc catttgcgat gctgattgga
     2101 aaattcgcgg gattcctgcg gacttagaag accgccgcgt agagataact ggcccggtag
     2161 agcgcaagat ggtgatcaac gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg
     2221 aagattcact ggccccggac tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg
     2281 cagttaacgg taccatcagc tataccaatg aagcaggcaa aatttaccag ctcaagccca
     2341 atccagcggt tttgatttgt cgggtacgcg gtctgcactt gccggaaaaa catgtcacct
     2401 ggcgtggtga ggcaatcccc ggcagcctgt ttgattttgc gctctatttc ttccacaact
     2461 atcaggctct gttggcaaag ggcagtggtc cctatttcta tctgccgaaa acccagtcct
     2521 ggcaggaagc ggcctggtgg agcgaagtct tcagctatgc agaagatcgc tttaatctgc
     2581 cgcgcggcac catcaaggcg acgttgctga ttgaaacgct gcccgccgtg ttccagatgg
     2641 atgaaatcct tcacgcgctg cgtgaccata ttgttggtct gaactgcggt cgttgggatt
     2701 acatcttcag ctatatcaaa acgttgaaaa actatcccga tcgcgtcctg ccagacagac
     2761 aggcagtgac gatggataaa ccattcctga atgcttactc acgcctgttg attaaaacct
     2821 gccataaacg cggtgctttt gcgatgggcg gcatggcggc gtttattccg agcaaagatg
     2881 aagagcacaa taaccaggtg ctcaacaaag taaaagcgga taaatcgctg gaagccaata
     2941 acggtcacga tggcacatgg atcgctcacc caggccttgc ggatacggca atggcggtat
     3001 tcaacgacat tctcggctcc cgtaaaaatc agcttgaagt gatgcgcgaa caagacgcgc
     3061 cgattactgc cgatcagcta ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc
     3121 gcgcaaatat tcgcgtagcc gtgcagtaca tcgaagcgtg gatctctggc aacggctgtg
     3181 tgccgattta tggcctgatg gaagatgcgg cgacggctga aatttcccgt acctcaatct
     3241 ggcagtggat ccatcatcaa aaaatgttga gcaatggcaa accggtaact aaagccttgt
     3301 tccgccagat gctgggcgaa gagatgaaag tcattgccag cgaactgggc gaagaacgtt
     3361 tctcccaggg gcgttttggc gatgccgcac gcttgatgga acagatcacc acttccgatg
     3421 agttaattga tttcctgacc ctgccaggct actgcctgtt agcgtaaacc accacataac
     3481 tatggagcat ctgcacatga aaacccgtac acaacaaatt gaagaattac agaaagagtg
     3541 gactcaaccg cgttgggaag gcattactcg cccatacagc gcggaagatg tggtgaaatt
     3601 acgcgattca gtcaatcctg aatgcaccct ggcgcaactg ggcgcggcga gaatgtggcg
     3661 tctgctgcat ggcgagtcga aaaaagctac atcaacagcc tcggtgcact gactggcggt
     3721 caggcgctgc aacaggcgaa agcgggtatt gaagcagtct atctgtcggg atggcaggta
     3781 gcggcggacg ctaacctggc ggccagcatg tatccggatc agtcgctcta tccggcaaac
     3841 tcggtgccag ctgtggtgga gcggatcaac aatactttcc gccgtgcgga tcagatccaa
     3901 tggtccgcgg gcattgagcc gggcgatccg cgctatgtcg attacttcct gccgatcgtt
     3961 gccgatgcgg aagccggttt tggcggtgtc ctgaatgcct ttgaactgat gaaagcgatg
     4021 attgaagccg gtgcagcggc agttcacttc gaagatcagc tggcgtcagt gaagaaatgc
     4081 ggtcacatgg gcggcaaagt tttagtgcca actcaagaag ctattcagaa actggtcgcg
     4141 gcgcgtctgg cagctgacgt gacgggcgtt ccaaccctac tggttgctcg taccgatgcc
     4201 gcggatctga tcacctccga ttgcgacccg tatgacagcg aatttattac cggcgagcgt
     4261 accagtgaag gcttcttccg tactcatgcg ggcattgagc aagcgatcag ccgtggcctg
     4321 gcgtatgcgc catatgctga cctggtctgg tgtgaaacct ccacgccgga tctggaactg
     4381 gcgcgtcgct ttgcacaagc tatccacgcg aaatatccgg gcaaactgct ggcttataac
     4441 tgctcgccgt cgttcaactg gcagaaaaac ctcgacgaca aaactattgc cagcttccag
     4501 cagcagctgt cggatatggg ctacaagttc cagttcatca ccctggcagg tatccacagc
     4561 atgtggttca acatgtttga cctggcaaac gcctatgacc agggcgaggg tatgaagcac
     4621 tacgttgaga aagtgcagca gccggaattt gccgccgcga aagatggcta taccttcgta
     4681 tctcaccagc aggaagtggg tacaggttac ttcgataaag tgacgactat tattcagggc
     4741 ggcacgtctt cagtcaccgc gctgaccggc tccactgaag aatcgcagtt ctaagcaaca
     4801 acaaccgttg ctgactgtag gccggataag gcgttcacgc cgcatccggc aatcggtgca
     4861 cgatgcctga tgcgacgctt gcgcgtctta tcatgcctac agccgttgcc gaacgtaggc
     4921 cggataaggc atttacgccg catccggcaa tcggtgcacg atgcctgatg cgacgcttgc
     4981 gcgtcttatc atgcctacaa ccgttgccga acgtaggctg gataaggcgt ttacgccgca
     5041 tccggcaatt ctctgctcct gatgagggcg ctaaatgccg cgtggcctgg aattactgat
     5101 tgctcaaacc attttgcaag gcttcgacgc tcagtatggt cgattcctcg aagtgacttc
     5161 cggggcgcag cagcgtttcg aacaggccga ctggcatgct gtccagctgg cgatgaaaaa
     5221 ccgtatccat ctttacgatc atcacgttgg tctggtcgtg gagcaactgc gctgcattac
     5281 taacggccaa agtacggacg cggcattttt actgcgcgtt aaagagcatt acacgcggct
     5341 gttgccggat tacccgcgct tcgagattgc ggagagcttt tttaactccg tgtactgtcg
     5401 gttatttgac caccgctcgc ttactcccga gcggcttttt atctttagct ctcagccaga
     5461 gcgccgcttt cgtaccattc cccgcccgct ggcgaaagac tttcaccccg atcacggctg
     5521 ggaatctcta ctgatgcgcg ttatcagcga cctgccgctg cgcctgcgct ggcagaataa
     5581 aagccgtgac attcattaca tcattcgcca tctgacggaa acgctgggga cagacaacct
     5641 cgcggaaagt catttacagg tggcgaacga actgttttac cgcaataaag ccgcctggct
     5701 ggtgggcaaa ctgatcacgc cttccggcac gttgcccttc ttactgccga tccaccagac
     5761 ggacgacggc gagttattta ttgatacctg cctgacgacg accgccgaag cgagcattgt
     5821 ttttggcttt gcgcgttctt attttatggt ttatgcaccg ctgcccgcag cgctggtcga
     5881 gtggctacgg gaaattctgc caggtaaaac cactgctgaa ttgtatatgg ctatcggctg
     5941 ccagaagcac gccaaaaccg aaagctaccg cgaatatctc gtttatctac ag
//
LOCUS       DAJTYD010000197         5939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTYD010000197 DAJTYD010000000
VERSION     DAJTYD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.199"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1034
                     /locus_tag="OO585_003562"
     CDS             <1..1034
                     /locus_tag="OO585_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6631016.1"
                     /translation="LSLSSVALAAPKGICTPDNGVFHSTLDFSGYLITANENKVGTTF
                     NTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHSNINYYDLNPNLDVG
                     IAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKAIVYFYIKKTFAGKL
                     IIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINNLQPVYFDFKEIPA
                     ADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNV
                     GIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTAT
                     ATITVEIVR"
     gene            1282..2322
                     /gene="pstS"
                     /locus_tag="OO585_003563"
     CDS             1282..2322
                     /gene="pstS"
                     /locus_tag="OO585_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6631017.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            2409..3368
                     /gene="pstC"
                     /locus_tag="OO585_003564"
     CDS             2409..3368
                     /gene="pstC"
                     /locus_tag="OO585_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6631018.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            3368..4258
                     /gene="pstA"
                     /locus_tag="OO585_003565"
     CDS             3368..4258
                     /gene="pstA"
                     /locus_tag="OO585_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6631019.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            4349..5122
                     /gene="pstB"
                     /locus_tag="OO585_003566"
     CDS             4349..5122
                     /gene="pstB"
                     /locus_tag="OO585_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6631020.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5137..5862
                     /gene="phoU"
                     /locus_tag="OO585_003567"
     CDS             5137..5862
                     /gene="phoU"
                     /locus_tag="OO585_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6631021.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1466 a         1413 c         1549 g         1511 t
ORIGIN      
        1 tgctctcgtt aagtagtgta gcacttgcag ctcctaaagg tatctgtacc cctgataatg
       61 gcgtatttca cagtacgctt gatttctcgg gatatcttat taccgcgaac gaaaataagg
      121 ttggaacgac atttaacacc actgtaacta atggatcctc atatcctgga cgctgtcatt
      181 gtgatactgg taacgtagga gaattcccct atatctatta tacgtcaaaa ataaaccagg
      241 cattaactta tgctggggtt cactctaata ttaattatta tgatcttaat ccgaaccttg
      301 atgttgggat tgctatagat attcttgggg ttgggtatgt gaatgcacct tttgaatatc
      361 atgctaacaa cccgtctggt aatacaaagt acaattgcaa ccgcattgaa cctttaagta
      421 tatctagtgg ggctaaagcg atagtatatt tttatatcaa gaagacattt gcaggaaaat
      481 tgattattcc tgaaacaaag atagtgacat tgtatggaac aattagccgt gacaccccgg
      541 tggattactc acagccgatg gctgatgttt atattcgggg cgatattaca gctccgcaaa
      601 gttgcgaaat aaataattta cagccagttt attttgattt taaagaaata ccagctgcag
      661 atttttcatc tgttgttgga agtgcggtaa taacacataa aattactaaa acggtcacta
      721 ttgagtgtga aaacctgggg atactgaata ccgatgatat cagtacgtct ttttatgcta
      781 ccgaacccaa tacggacaat tcaatggtcg taacttcaaa ctctaacgtg gggataaaaa
      841 tttacgacaa aaataataag gaaatcaaag tgaacggtgg tgagttgcca acagacatgg
      901 gtaaatcaac agtgtatggt gaaaaatcag gtagtgtaac tttttcagct gctcctgcga
      961 gccttactgg agctcgccca gccccggggc aatttactgc aacagcgacg ataactgttg
     1021 agattgtgcg ctaataggtc tgacaacgat tgttcatcac atcgttgtca tctcacccat
     1081 ttctttatca cttttaaatg catcatgaca aaaagatgac atgtgggatt ttcatcttca
     1141 catttaaaat cagtaagtta tttggtaatt cctttctgtc ataaaaatgt catattcctt
     1201 acatataact gtcacctgtt tgtcctattt tgcttctcgt agccaacaaa caatgcttta
     1261 tgaatcctcc caggagacat tatgaaagtt atgcgtacca ccgtcgcaac tgttgtcgcc
     1321 gcgaccttat cgatgagcgc tttctctgtg tttgcagaag caagcctgac aggtgcaggt
     1381 gcaaccttcc ctgcgccggt gtatgccaaa tgggctgaca cttaccagaa agaaaccggt
     1441 aataaagtta actaccaggg tatcggttct tccggtggcg taaaacagat tatcgctaat
     1501 accgttgatt ttggtgcctc tgacgcgccg ctgtctgacg aaaaactggc tcaggaaggt
     1561 ctgttccagt tcccgaccgt gattggcggc gtggtgctgg cggttaacat tccagggctg
     1621 aagtctggcg aactggtgct ggatggtaaa accctcggcg acatctacct gggcaaaatc
     1681 aagaagtggg atgatgaagc catcgccaaa ctgaatccgg gtctgaaact gccttcacaa
     1741 aacattgctg tagtacgccg cgcagatggc tccgggactt ccttcgtctt caccagctac
     1801 ctggcgaaag tgaacgaaga gtggaaaaac aacgttggta ctggctctac cgtaaaatgg
     1861 ccgatcggtc tgggcggtaa aggtaacgac ggtatcgccg cgttcgttca gcgtctgccg
     1921 ggtgcaattg gttatgttga atatgcttac gcgaagcaga acaacctggc gtacaccaaa
     1981 ctgatctccg ctgatggtaa accggttagt ccgaccgaag aaaacttcgc taatgcagca
     2041 aaaggtgcag actggagcaa aaccttcgct caggatctga ccaaccagaa aggcgaagat
     2101 gcatggccta ttacctctac cacgttcatt ctgatccaca aagatcagaa gaaaccagaa
     2161 caaggcacag aagtgctaaa attcttcgac tgggcgtaca aaaccggggc taaacaggcg
     2221 aacgacctgg attacgccag cctgccggat agtgtagttg aacaggttcg cgctgcgtgg
     2281 aagaccaata ttaaagacag tagcggtaag ccgctgtact aataaaactc caggccgggt
     2341 acggtgtttt acgccgcatc cggcattaca aaatgacttt gtaaacgcgt ttaactgaag
     2401 agtaacttat ggctgcaacc aagcctgctt ttaacccacc gggtaaaaag ggcgacataa
     2461 ttttcagcgt gctggtaaaa ctggcggcgc tgattgtgct attgatgttg ggtggcatta
     2521 ttgtctctct gatcatctcc tcctggccga gcattcagaa atttggtctg gctttcctat
     2581 ggaccaaaga gtgggatgca ccgaacgata tctacggggc gctggtgccg atctacggta
     2641 cgttggtgac ttcgtttatc gcgctgctga tcgccgtccc ggtgagtttc ggtatcgccc
     2701 tgttcctgac tgagcttgcg cctggctggc tgaaacgccc gctgggtatc gccattgagc
     2761 tgctggcagc cattccaagt atcgtttacg gcatgtgggg cctgtttatc tttgcgccgc
     2821 tgttcgccgt ttactttcag gagccggtcg gcaatatcat gtcgaatatc ccgattgttg
     2881 gcgcgctgtt ctctggcccc gcatttggta tcggtatcct cgcggcaggc gtgatcctcg
     2941 ccatcatgat tattccgtac attgcggcgg taatgcgtga tgtgttcgaa caaaccccgg
     3001 tgatgatgaa agagtcggcc tacggtattg gctgcaccac ctgggaagtt atctggcgta
     3061 tcgttcttcc gttcaccaaa aatggtgtta tcggcggcat catgctgggg ctgggccgcg
     3121 cgctcggtga aaccatggcg gtgaccttta tcatcggtaa cacctaccag ctcgacagcg
     3181 cctcgctgta catgccgggc aatagtatca cctctgcgtt ggcgaacgaa tttgcggaag
     3241 cggaatccgg tctgcacgtt gccgcactga tggaactggg cctgatcctg tttgtgatta
     3301 ccttcatcgt cctcgccgca tcgaagttta tgattatgcg cctggctaag aatgaggggg
     3361 cacgctaatg gctatggttg aaatccaaac cactgcggcg ctggctgaat ctcgccgcaa
     3421 aatgcaggcg cgtcgccgcc tcaaaaaccg tattgcgctg acgctctcga tggcgacgat
     3481 ggccttcggc ctgttctggc tgatctggat tttaatgtcc accatcactc gcggtatcga
     3541 cggtatgtcg ctggcgctgt tcactgaaat gacgccgccg cccaatacgg aaggtggtgg
     3601 tctggcgaac gccctggcgg gtagcgggct gttaattttg tgggccacgg tattcggtac
     3661 gccgctgggc attatggcgg ggatttatct ggcggaatat ggtcgtaaat cctggctggc
     3721 agaagtgatt cgcttcatta acgacattct gctctccgcg ccgtcgattg tggttggtct
     3781 gtttgtttac accatcgtgg tggcgcagat ggagcacttc tctggctggg cgggcgtgat
     3841 tgccctggcg ctgttgcagg tgccgattgt tatccgcacc accgagaaca tgctgaaact
     3901 ggtgccggac agcctgcgtg aagcggctta tgcgctgggt acaccgaagt ggaagatgat
     3961 ctctgcgatt acgctgaaag cgtcggtgtc cgggatcatg accggtatcc tgctggcgat
     4021 tgcccgtatt gctggtgaaa ccgcgccgct gctgtttacc gcgctctcca accagttctg
     4081 gagcacggac atgatgcagc cgatcgccaa cctgccggtg acgatcttta agtttgcgat
     4141 gagcccgttt gcggaatggc aacaactggc ctgggccggg gtattgatta ttaccctgtg
     4201 cgtactgctg ctgaacattc tagcgcgcgt tgtttttgcg aagaataaac acggttaaca
     4261 ctttgccgga tgcggcgtga acgccttatc cggcctacgg taagcctgat tagcgaagtg
     4321 catcaggcac gatgaggaaa agattgcaat gagtatggtt gaaactgccc cgagtaaaat
     4381 tcaggttcgt aatttgaact tctactacgg caaattccat gccctgaaaa acatcaacct
     4441 ggatatcgct aaaaaccagg ttacggcgtt tatcgggccg tccggctgcg gtaaatcgac
     4501 gctgctgcgt accttcaaca aaatgtttga actgtacccg gagcagcgtg cggaaggtga
     4561 aattctgctt gatggcgaca acatcctgac caactctcag gatatcgcac tgctgcgtgc
     4621 gaaagtgggc atggtgttcc agaaaccgac gccgtttccg atgtccatct acgacaacat
     4681 cgcttttggc gttcgtctgt ttgagaagct ctcccgtgcc gacatggacg agcgcgtgca
     4741 gtgggcattg accaaagccg cattgtggaa cgaaaccaaa gataaattgc accagagcgg
     4801 ttactctctc tctggtggtc agcaacagcg tctgtgtatt gcgcgtggta tcgccattcg
     4861 cccggaagtg ctgctgctcg acgaaccgtg ttcggcgctc gaccctatct ctaccgggcg
     4921 tattgaagag ctgatcaccg aactgaagca ggattacacc gtggtgatcg tcacccacaa
     4981 catgcagcag gctgcgcgtt gttccgacca cacggcgttt atgtacctgg gcgaattgat
     5041 tgagttcagc aacacggacg atctgttcac caagccagcg aagaaacaaa cagaagacta
     5101 catcaccggt cgttacggtt gattcaggag tgcgttatgg acagtctcaa tcttaataaa
     5161 catatttccg gccagttcaa cgccgaactg gaaagtatcc gcacgcaggt gatgaccatg
     5221 ggcggcatgg tggagcagca gctttctgat gcaatcaccg cgatgcataa ccaggacagc
     5281 gatctggcga agcgcgtgat cgaaggcgac aagaacgtca acatgatgga agtggcgatc
     5341 gatgaagcct gcgtgcgcat tatcgccaaa cgtcagccga cggcgagcga cctgcgactg
     5401 gttatggtga tcagtaaaac cattgccgag ctggagcgta ttggcgacgt ggcggacaaa
     5461 atctgccgta ctgcgctgga gaaattctcc cagcagcatc agccgttgct ggtaagtctg
     5521 gagtcgctgg gccgtcatac catccagatg ctgcacgacg tgctggacgc gttcgcgcgg
     5581 atggacattg acgaagcggt acgtatttat cgtgaagata aaaaagtcga tcaggaatac
     5641 gaaggtattg ttcgtcaact gatgacctac atgatggaag attcgcgtac cattccgagc
     5701 gtacttactg cgctgttctg cgcgcgttct atcgaacgta ttggcgaccg ctgccagaat
     5761 atttgtgagt ttatcttcta ctacgtgaag gggcaggatt tccgtcatgt aggcggtgat
     5821 gagctggata aactgctggc gggaaaagat agcgacaaat aattcaccag acaaatccca
     5881 ataacttaat tattgggatt tgtggtaatg ctgccaactt actgatttag tgtatgatg
//
LOCUS       DAJTYD010000198         5925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTYD010000198 DAJTYD010000000
VERSION     DAJTYD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.200"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(92..622)
                     /locus_tag="OO585_003568"
                     /pseudo
     CDS             complement(92..622)
                     /locus_tag="OO585_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(965..1615)
                     /gene="zinT"
                     /locus_tag="OO585_003569"
     CDS             complement(965..1615)
                     /gene="zinT"
                     /locus_tag="OO585_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6631022.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1872..2507)
                     /gene="msrQ"
                     /locus_tag="OO585_003570"
     CDS             complement(1872..2507)
                     /gene="msrQ"
                     /locus_tag="OO585_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6631023.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(2508..3512)
                     /gene="msrP"
                     /locus_tag="OO585_003571"
     CDS             complement(2508..3512)
                     /gene="msrP"
                     /locus_tag="OO585_003571"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6631024.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(3621..4034)
                     /gene="hiuH"
                     /locus_tag="OO585_003572"
     CDS             complement(3621..4034)
                     /gene="hiuH"
                     /locus_tag="OO585_003572"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6631025.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4167..4838
                     /gene="hprR"
                     /locus_tag="OO585_003573"
     CDS             4167..4838
                     /gene="hprR"
                     /locus_tag="OO585_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6631026.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4838..>5925
                     /gene="hprS"
                     /locus_tag="OO585_003574"
     CDS             4838..>5925
                     /gene="hprS"
                     /locus_tag="OO585_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6631027.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIR"
BASE COUNT         1669 a         1210 c         1366 g         1680 t
ORIGIN      
        1 gttgatagtg gaaagggtgc attgtgcact tgcgtaaaca tttatgacgc gttgatgtcc
       61 atggttcaca tttccttgtc aggggatcga cttatttgcg gcgaggcatc attcgtaaca
      121 gagtattatc tttctgaatg tagtgatgaa agagtgctgc gccagcgtga gctgcgatta
      181 aaaagtaacc tatatttgcc caggtttcgt gaatatttta tatcagtgct ttaatttcgc
      241 tgtttggggt aacaaaggga gacacattga aaccaaggaa actccacgat tttccactgt
      301 aagccatcaa agcaatcccc agcagaggaa gtgcaaggaa ggttatataa agcatgatgt
      361 gcattagcga agccgctttc atttgccagg caggtggcgg tggcacaata gaaggatcac
      421 tataacggtg ttttataatc aggcgtgaaa acattaacac ccaaacgaat atacccgcat
      481 tgtaatgtgt ttctcgcatc agcagataag tactactacc tttaggaaac cagccacgga
      541 gttccatcgc ggcataggtt attgcaacga aaagcagcgt tatccaatgt aaataaattt
      601 gagtttttga gaatcggttc atttttgtag ctcctgtaat ttttacagtc aagctatccc
      661 acgaacctta aaaattcctt atacagatgt ggttttaaga atttgttatt taaaaaattg
      721 atagtgcaca ttgtaaccag ccaatataag caatggcgtg agcacaggta tctttctctg
      781 ttggccgtat tgtgtatgga atccgttatt ggttaactca atgcgggtta gcttattaat
      841 atacagacac gtcatagtga tgagcgtgtc tgtagctagc ttgcgttcag tggcgtttgt
      901 ggatggacca gtggcagtta tgatgactta ctaaagcggc atcgaggcgt tatcatgaga
      961 atactcaatg agacatcatt tcctcgacca cttcttcgct actcaactga tatggataat
     1021 acgttggcca gttttccatt tcatttaata aagattgctg tgaatcatta cccataaaaa
     1081 tgtgaaaatg agatgatttt cgtggtgcaa ttatatgatc gctaaattgt atatatttag
     1141 gggctttgct ttcaggatct ttacattcga ataagtaacg aacgcctttc ttgcctgatt
     1201 tataggtgag tattttgtat ccatcgtaat catatttaca ggatgttgtt tcattatttc
     1261 tatggaattc aacaatgccg tcctcaatgc caatcatctc gatatctgtt gcataacctt
     1321 tgtgataata atctttaatt tcagcaaatg ttttagtttt atctgcatcc gctttcttct
     1381 gaaagacggg gtcaagtttg ccactttgca gtaaaggata aacggattgc cagaccccat
     1441 cccagtcact gagcgttcgg ttttgtacat tggcatcatc aaaaacacca ttagcagctt
     1501 tttgttcgac ctctgttaag ggtttgccgt gtgagtgatg accatgcgaa aaggcaggag
     1561 cgctaacaat aaagacacct aacgcaacag ccagtttgtg aagacgaatc gccaaaacag
     1621 tttcctccag agtcattgta tatgatgtgt aatgttatat tgtaacatat ggcaaaatgc
     1681 aacaactctt aggagatgag agcatagttt tctcaggtgt acttatctat cttattgaaa
     1741 taaaatgaat tatattgata atttgaggtt gatgacaaac gaaacataat ctgatgtgat
     1801 ctgcgtaggc cggatagggc gctcgcgccg catccggcag ggataacgcg tactttgtcg
     1861 gcaatatgaa gttatacaga taatttgttg tgaacctgct tgcgtaaccg gttaaaaagg
     1921 gaacgcaact tcttataccg taaggctaaa agcagtacag ccagcccagc gtagatgagc
     1981 ggctgcggtg aaataatctt cactgaccac agataatgta ttggggccag gatcgcgaca
     2041 agatagacga aattatgcaa ctgttgccag tgcttgccga gttttcgctg catcgcctgg
     2101 gttgaagtaa acgctaaagc aagcaaaata acccagctga taattcctaa cgttaaataa
     2161 ggccgggtaa ttaactcctt gcctaataac gccagattat tcacgcccag ctccagcaat
     2221 gcgtaactgg ttaaatgcag tgtcgcccag gcaaagcacc ataagcctaa caggcggcga
     2281 gtgcgtatca ataacggctg ttttgcgtag cgtgccagag gtgtgattaa caaggtcgcc
     2341 agcaaaaatt tcagtgcagt gcgaccggta aaatgctgaa tatctttcac cggatcggca
     2401 cccagtccac cgtgattgat cgcccagacc agccagagaa acggcaacaa tccggcaaga
     2461 tgcaggctaa ctttcagcca tgtcacctgt tttgctgtca gacgcattca gaaattctcc
     2521 cgcaaatcca ggccacgata cagcgatgcc acctggtcgg cgtaaccatt aaacagtagc
     2581 gttggctggc gctgaacatc gaggatgccg cctgaaccaa taaatcgttc ggtagcctgt
     2641 gaccagcgcg ggtgatcaac atgcggatta acgttggcgt aaaaaccgta ttcgtcaggc
     2701 gctgccagat tccaggtggt tggcggacgc tcgcgggtca gcttaatact gactatcgat
     2761 ttaatccctt taaagccata tttccacggc acaatcagtc gtaccggcgc gccattttgt
     2821 ggcggtaacg ccttgccata aacacccacg gtcatcagtg tgagcggatg cattgcttcg
     2881 tcgagacgca atccttcgac ataaggatat ttcagcccgc cgccgataaa gcggtcctgc
     2941 tggcctggca tttgttccgg tgcataaatt gtttcgaaag cgacatactt cgcattgctg
     3001 gtgggttcgg caagcgccag caatttgtgc agcggaaaac caatccacgg caccaccatc
     3061 gaccatgcct ccacgcagcg catacgataa atacgctctt ccagcgggaa gcgacgggtt
     3121 aaatcatcgt gatcgagggt caatggtttt gccacttcgc cgctgatttt cagtgtccat
     3181 ggatcggttt tcaggctacc agcattagcg gcgggatcgg ctttatccag cccgaattca
     3241 tagaagttgt tataaccgga gactttatct actggcgtca gtggcaggtt attttgccag
     3301 gcggcaggct tgctgaactc cagcggtttt ccggcggggg cgggcgggcg atcgttccct
     3361 ttaaaccagc taagcagatc ggcatgcgca gcgtgaggca aagaaagtgc agctgcactg
     3421 atgcccagtg cttttaacac ctgccgacgc ttcataaaga ataccgactc ggccgtaaca
     3481 tctgattctt ttaaaaattg attctttttc ataagccatc acactttttt gtcgttagcc
     3541 agcaagagcc gtttactgtt aatctgtcag ggagtgtcct ccctaacagt agcagagatc
     3601 tgcttttaca tatttatgca ttaactgcca cgataggttg aatacccata ttggctaagt
     3661 aataaaggca catgataatg ctcgttcact ttattaatat gaaattcaac ggggatctca
     3721 gggaagaaac tttcaagatt ttgtttcttg aaatagtccc cggttttaaa tacgacacgg
     3781 taatcgcccg tagttgcagt ttgctcgggc cacagtgctt taattcgtcc atccttatct
     3841 gttttggcgg tattaagttg taaccagccg ttatccgcct tcttttcaag agtgactgtc
     3901 acgtcagcag cgggctttcc ggtttgctgg ttcaaaatgt gcacgctaag aatgttttgt
     3961 tgtgcggcat aaaccaaaga aggtaatgaa aatgctgccg ttgctaccga gagtactaaa
     4021 taacgcttta acatgtttat atccttgtca tgtgaatgag ttttgaggat agcgaaaaag
     4081 cggccagggt aaccttacag cagcattaca attttgtaat gaaccaggct gtttctataa
     4141 catatgattt atggcatatt attttcatga agattttact tattgaagat aatcaaagga
     4201 cccaggagtg ggtaacgcag gggctttccg aagcgggtta tgtcattgat gccgtttctg
     4261 atggcagaga tgggctttat cttgcgctga aggatgatta tgcattgata attctggata
     4321 ttatgcttcc gggtatggat ggctggcaga tcttacaaac gttaagaaca gcaaagcaaa
     4381 cccctgttat ttgccttact gcaagggatt ctgtcgatga cagagtcaga gggctggaca
     4441 gtggagcaaa tgattatctg gtaaaacctt tttcattttc tgagttgctg gcaagggttc
     4501 gggcacaatt aaggcaacat cacgctttga attcaacatt agaaatcagt ggcttaagaa
     4561 tggactctgt aagtcaaagt gtgagcaggg acaatatcag tattacactg acgcgcaagg
     4621 agtttcagtt actttggcta ctggcctcca gagctggcga aattataccc agaacggtta
     4681 ttgcgagtga aatttgggga atcaactttg atagtgatac caatacggtg gatgtcgcca
     4741 ttcgcaggct ccgcgcaaaa gttgatgatc cttttcctga aaagctaatc gccacaatcc
     4801 gggggatggg ctattcattc gtagcggtaa aaaaataatg aaaagattgt ctataaccgt
     4861 ccgtttaacc ttgcttttta tattgctgct gtctgttgct ggcgctggaa ttgtctggac
     4921 tctctataat ggcctggcaa gtgagttgaa atggcgcgat gatacaacac tcattaaccg
     4981 gacagcacag atcaagcagt tgttaattga tggggtaaat ccagatacgt tacctgtata
     5041 ctttaaccgg atgatggatg ttagtcagga tatcttgatc attcatggtg atggcatcaa
     5101 taaaattgtt aaccggacaa atgtcagtga tgacatgtta aataacatac ctgctagtga
     5161 gacaatcagc gcagctggca tttacagaag cattattaat gatactgaga ttgatgcttt
     5221 acgaattaat attgatgaag tttcgccatc attaacggtt actgtggcta aattggcttc
     5281 agctagacat aacatgcttg aacagtataa aattaatagc attataattt gcattgtcgc
     5341 cattgtactt tgctcagtat taagtccgct gttaatcaga acgggattac gagagatcaa
     5401 aaagttgagt ggtgtaacgg aagcgctgaa ttataacgat agccgagagc ctgttgaggt
     5461 tagcgcatta ccgagagaac taaaacctct tgggcaggcg ttgaataaaa tgcatcaagc
     5521 cttagtcaaa gattttgaac gcctaagtca atttgctgac gatctcgctc atgaacttag
     5581 aacgcccatt aatgcattac tgggtcagaa tcaggttacg ctcagtcaaa ccagaagtat
     5641 cgctgaatat caaaaaacaa ttgccggtaa cattgaagag ttggaaaata tttcgcggtt
     5701 aacagagaac atactgtttc ttgcccgggc agataaaaac aatgttttgg tgaaactgga
     5761 ctcgctttct ctcaataagg aagtcgaaaa tttgttggat tatcttgaat atctttcaga
     5821 cgagaaagag atttgcttta aggtcgagtg caatcagcaa atctttgcgg ataaaatttt
     5881 actgcaacga atgttatcga atcttattgt taatgccatt agata
//
LOCUS       DAJTYD010000199         5875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTYD010000199 DAJTYD010000000
VERSION     DAJTYD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.201"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(83..619)
                     /gene="ssb1"
                     /locus_tag="OO585_003575"
     CDS             complement(83..619)
                     /gene="ssb1"
                     /locus_tag="OO585_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6631028.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            873..3695
                     /gene="uvrA"
                     /locus_tag="OO585_003576"
     CDS             873..3695
                     /gene="uvrA"
                     /locus_tag="OO585_003576"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6631029.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3730..4086)
                     /locus_tag="OO585_003577"
     CDS             complement(3730..4086)
                     /locus_tag="OO585_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6631030.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLRPEEKRKLLVQL"
     gene            complement(4090..4506)
                     /locus_tag="OO585_003578"
     CDS             complement(4090..4506)
                     /locus_tag="OO585_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6631031.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLANMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4617..5330)
                     /gene="aphA"
                     /locus_tag="OO585_003579"
     CDS             complement(4617..5330)
                     /gene="aphA"
                     /locus_tag="OO585_003579"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6631032.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5693..>5875
                     /locus_tag="OO585_003580"
     CDS             5693..>5875
                     /locus_tag="OO585_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631033.1"
                     /translation="MVINFITRDGDDDMNISYMNSNKTTSLPVELDALNNKDISYAKD
                     FSYVKDFFLYIETLLKI"
BASE COUNT         1436 a         1501 c         1494 g         1444 t
ORIGIN      
        1 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
       61 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
      121 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
      181 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
      241 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
      301 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
      361 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
      421 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
      481 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
      541 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
      601 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
      661 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
      721 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
      781 cattcaggtc aatttgtgtc ataattaacc gtctgtgatc gccggtagca ccatgccacc
      841 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
      901 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
      961 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     1021 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     1081 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     1141 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     1201 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     1261 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     1321 tactcgcgcc aatcattaaa gaacgcaaag gcgaacacac caaaacgctg gagaacctgg
     1381 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     1441 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     1501 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     1561 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     1621 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccacga ctgttttcgt
     1681 ttaacaaccc ggcaggtgcc tgcccaacct gtgacggcct tggcgtacag caatatttcg
     1741 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     1801 gggatcgccg caacttctat tacttccaga tgctgaaatc gctggcagat cactataagt
     1861 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     1921 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccatcc
     1981 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacagaat
     2041 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     2101 aagggacgcg tctgcgtcgg gaagcgcgcc acgtttatgt cgagaatacg ccgctgcctg
     2161 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactagctg
     2221 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     2281 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     2341 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     2401 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     2461 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     2521 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     2581 aagtggtcgc agaaggtccg ctggaagcga tcatggcggt gcctgaatcg ttgaccgggc
     2641 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     2701 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     2761 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     2821 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     2881 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     2941 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     3001 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     3061 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     3121 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     3181 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     3241 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     3301 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     3361 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     3421 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     3481 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     3541 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     3601 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     3661 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     3721 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtcgta aattcactgc
     3781 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     3841 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     3901 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     3961 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     4021 gttatgcacg ctctgttctg cgcctagttt tgccatgcaa tattgtagca actccgaaat
     4081 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     4141 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     4201 caatacaagc gatgttccct gcattgagga tttgatatga gaaggcatat cgtctgctcc
     4261 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     4321 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     4381 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata ttagccagct gattcagaat
     4441 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     4501 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     4561 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     4621 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     4681 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     4741 atatcattat cagaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     4801 tttgtatttt gccctggttt atcgcccgca aagatcaccg gattcatgtt ggtggcagga
     4861 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg actacgacca
     4921 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     4981 tcttttggaa tgctgaattc atcccagcca ttgttcattt tttcccagaa cacaggattt
     5041 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa gcccgggctg
     5101 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     5161 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     5221 acgttagtac cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     5281 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     5341 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     5401 aacgccagct gcaggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     5461 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     5521 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     5581 tttgatagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     5641 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgat
     5701 taattttatt actcgcgatg gagatgatga tatgaatata tcatatatga atagcaacaa
     5761 aacgacatct ttacctgttg agctagatgc actaaataat aaagatatct cttatgccaa
     5821 agatttttct tatgtcaaag atttcttttt atacatagaa acactgttaa aaatt
//
LOCUS       DAJTYD010000200         5871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTYD010000200 DAJTYD010000000
VERSION     DAJTYD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.202"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(23..604)
                     /locus_tag="OO585_003581"
     CDS             complement(23..604)
                     /locus_tag="OO585_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631034.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(651..4508)
                     /gene="sigA"
                     /locus_tag="OO585_003582"
     CDS             complement(651..4508)
                     /gene="sigA"
                     /locus_tag="OO585_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6631035.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4869..>5871)
                     /locus_tag="OO585_003583"
     CDS             complement(4869..>5871)
                     /locus_tag="OO585_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCR6631036.1"
                     /translation="ILLPSVGAQLSNPDNITDNADRISDEWKAFDRAIESNHGVPEAA
                     ARLKEHLRDFRNTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSVDGKVSDADLTYL
                     EEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWSLGFPPKFQRVDL
                     FNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTNLDDNWTLGLVAQNLIPRSIETK
                     EVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNRQFAAIGAEFNAW
                     KWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNTYGAVAQFQFTF"
BASE COUNT         1452 a         1305 c         1305 g         1809 t
ORIGIN      
        1 ggctaaaata ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa
       61 gtactgagct tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc
      121 cctgaaataa tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca
      181 tatttttaat gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac
      241 tgagtctgat gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt
      301 cagtgaccag catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg
      361 tgattgtaat gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc
      421 cgtatcggaa ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt
      481 tttgcctgat agtttctttt tttattcgct catgtttaag gcataacagg cagataaata
      541 atgtcagaaa aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat
      601 acatattctc tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag
      661 taacggaagt tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca
      721 aactcaagac caaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga
      781 atacgaccgt ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt
      841 tcaccgttgg caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag
      901 tccttaccgg agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag
      961 tccttatcct tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat
     1021 acggccccat aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag
     1081 cggtagcctg cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagc
     1141 ccagcaaagg tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata
     1201 taggcaccag agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg
     1261 ccactgaatg cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa
     1321 aggtcaagtc catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg
     1381 tcactgtatc caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccgacc
     1441 tcgccattgg tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg
     1501 aaggctttgt aattaccgga catatacgat tttgctgcac tggaagatgc tgtattttca
     1561 gccaccttat atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc
     1621 acaggagata tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg
     1681 cttccttttg gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc
     1741 tgtatatcga ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta
     1801 tccgattgat ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca
     1861 tccagtgtgt ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa
     1921 agactgtcgc gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg
     1981 ttatacatgc tggctgaggc agcaggagca tcaatacctc cggtataaga agtatcaaag
     2041 ccacccagca aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta
     2101 tactggtcaa caccaaacag aatagatgct tttttgtctg attttatatt accattgacc
     2161 caggcataat tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac
     2221 atatagattg ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt
     2281 ccggccagtt caatagcttt atctacatac atttttccct gggcggtaac agtagctcct
     2341 ttgttgataa tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat
     2401 ttatcaccaa cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg
     2461 cctgctgaat ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt
     2521 cttcctgaga acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta
     2581 gcctctatgt gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa
     2641 tttaatttcc ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc
     2701 tgattgttgg acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt
     2761 atggcagcag aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct
     2821 ttaaatatgg catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag
     2881 atatcgtttt tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc
     2941 tgggtagtat tgataatatg atttatgctt atattacctg atacattccc atggtacata
     3001 tatgcatcag tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt
     3061 gttcccgcat ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt
     3121 gttccgccat ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg
     3181 gcatttattt ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca
     3241 gcactaagga tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca
     3301 tccagagtac cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat
     3361 tcaacggtgg tattattatc aatatctatc ccggcacctt tataagactt atcttttccg
     3421 tacactttat atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc
     3481 tgcttgagcg ttaattcccc tcctcctgag acagacaggt ccttatcttt tataatcgat
     3541 gataactctt gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg
     3601 tttaccagaa tattattaaa taacgagtag tttgtccagg ctatattggt actactgata
     3661 aaaccaactg tactggtaac accatggaaa acccattttt gttgttcttt atcatataga
     3721 taagaaccag aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta
     3781 taaatgtaac tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga
     3841 ttatagtttt gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca
     3901 cctgctctga agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc
     3961 gcatcttcgg gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg
     4021 aatttattca gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat
     4081 gctgtctgat cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca
     4141 gtaacactgt acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa
     4201 tcaggtacag gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca
     4261 tttgttgcac ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc
     4321 catatattat ttatatccag cattgctgca aatgctgttt caccataact tccatatatt
     4381 gaggataaaa taataccgag gataactttc tttcttgatg taccgacact aactttccgg
     4441 gtcagttcag aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt
     4501 ttattcatac gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca
     4561 acggggaact atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt
     4621 tatatttgta tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg
     4681 tcagatattg atcacctggt gtttgtattt tgacctttgc gatcaaaatt tgatctttgt
     4741 tgtttccggt caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat
     4801 gtccgacaga cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca
     4861 ggtggaactc agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg
     4921 atcaggcctg caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg
     4981 aatgcactgc cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat
     5041 ttccaggcat taaattctgc gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa
     5101 ccgctggccg gggtcagatc cacatcaaat gcggtggtga acatcgcgtt gtgccaggag
     5161 acaccggccg tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt
     5221 gtctcaatac tacgggggat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc
     5281 aggttcgtac tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg
     5341 cctttgaagg cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg
     5401 cgctggaatt tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta
     5461 gccagagcaa tacccacatc agtgatcagt gccgcgcggg caaaggcctg tgatgtcagg
     5521 cggcttttgt ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca
     5581 gcatcagaga ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca
     5641 gagaggcgat caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt
     5701 gtgttacgga agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg
     5761 ttactttcga tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg
     5821 gtgatattat ccggattgga gagctgcgcg ccgacggacg gaagcaggat g
//
LOCUS       DAJTYD010000201         5757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTYD010000201 DAJTYD010000000
VERSION     DAJTYD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.203"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..1059
                     /locus_tag="OO585_003584"
                     /pseudo
     CDS             1..1059
                     /locus_tag="OO585_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1447..1823)
                     /gene="rnpB"
                     /locus_tag="OO585_003585"
     ncRNA           complement(1447..1823)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO585_003585"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1856..3001)
                     /gene="garK"
                     /locus_tag="OO585_003586"
     CDS             complement(1856..3001)
                     /gene="garK"
                     /locus_tag="OO585_003586"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6631037.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTVL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3098..3988)
                     /gene="garR"
                     /locus_tag="OO585_003587"
     CDS             complement(3098..3988)
                     /gene="garR"
                     /locus_tag="OO585_003587"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6631038.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4018..4788)
                     /gene="garL"
                     /locus_tag="OO585_003588"
     CDS             complement(4018..4788)
                     /gene="garL"
                     /locus_tag="OO585_003588"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6631039.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4804..>5757)
                     /gene="garP"
                     /locus_tag="OO585_003589"
     CDS             complement(4804..>5757)
                     /gene="garP"
                     /locus_tag="OO585_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6631040.1"
                     /translation="PSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWL
                     TFAWGWEHVFTVMGVIGFVLTALWIKLIYNPTDHPRMSAEELKFISENGAVVDMDHKK
                     PGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILK
                     VGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTN
                     NTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIG
                     YLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1524 a         1543 c         1278 g         1412 t
ORIGIN      
        1 aaactaaaac agacctctat tcaaatggtc actttactta acgcgttaca accaggtgga
       61 aaatgtatta tcactggtga ttttcaaaaa gaattagcgt acttacaaaa tgtaattctt
      121 tataatgact cgtttcttcg tctggatttt ttaggttata acgcccaaat tattcaacga
      181 tcggacaata cctgcgaact taccattaat gaaccgttaa aaaaccagga aatatctaca
      241 ggcaatatca atgttaattg tccattaaaa gatatttaca atgaaatcag gaggttgaac
      301 gcaaccttta gttgtgggac tggagatata gttgatctat cctctctgga cttacgtaat
      361 gtcgatttag attattatga tttcacagat aaacatatgg ctaatactat tctaaatcct
      421 tttaaattgg attcaacaaa ttttactaat gccaacatgt ttcaggttaa ttttgttagt
      481 tcaacacaaa acgccacaat ctcctgggat tatttactaa aaataacgcc tgttttaata
      541 agtattagcg atatgtattc tgaagaaaaa atcaagtttg tcgaaagttg tttaaatgag
      601 cttggagaca ttaccgaaga acaattaaaa attatgagat ttgcaattat aaaatctata
      661 ccaagggcaa ctcttacaga taaattagaa aatgaattaa caaaagaaat atataaaagc
      721 tcatcgaaaa tcaacaattg cttgaacaga attaaattac cagagatgaa agaattctca
      781 tcaaaaaaaa acatatgatt acatcgatat aatcattgaa gattatgaaa atatcaaaga
      841 aaatgcttat ctggtcgtcc cccaaattaa ttatactatg gatttaaaca tagaagactc
      901 cagctcagaa gagttaatac cctcgagaaa gacgaaaatt cttcggacaa tggctttgaa
      961 gtcgaagaat ataacgcata tgaagcatat aactcagaga atctatattt taccagagag
     1021 gaatatacgt atgattacga cctttcaaat gcaatataga cttaataact tcttacaaaa
     1081 tctataatta ataccgtaga tattcattca tcttaccctg tgcaccaaaa gcaaaaactc
     1141 actgacgcgg gtttgaaact gatggcaacc gcaaaaatgc ctgatgcgct acgcttatca
     1201 ggcctacgcc atctctgcaa tatattgaat ttgcgtgctt ttgtaggcag gataaggcgt
     1261 tcacgccgca tccggcatga ataaagcgca ctcaacaatc tgaaacccgc cggaacggtt
     1321 tattacgtac atcaggtgaa actgaccgat aagccgcttt cttttgggta tagtgtcgtg
     1381 gacagtcatt catctttctg cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg
     1441 taaatcaggt gaaactgacc gataagccgg gttctgtcgt ggacagtcat tcatctaggc
     1501 cagcaatcgc tcactggctc aagcagccta cccgggttca gtacgggccg taccttatga
     1561 acccctattt ggccttgctc cgggtggagt ttaccgtgcc acggactgtt accagccgcg
     1621 cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc cgcttgcgcg ggccatcggc
     1681 ggtttgctct ctgttgcact ggtcgtgggt ttccccccca ggcgttacct ggcaccctgc
     1741 cctatggagc ccggactttc ctcccctccg cccgtctccc ccgaagagga cgacgacgaa
     1801 gcggcgactg tctggtcagc ttcggcgcgc agtatagagg gtttgcgcgc ccttgtcacc
     1861 ccgcgttgcg cattccaatc gccagtgtcg cggcgatatt acgtgaagca cggcagatat
     1921 tgtcataagc cccgcggaat gcttcgtcca acgtacctat gctggtcaat acgctgaaga
     1981 ccgcatcaat gccatgctga tgtacaacgc caacatcatc ggtcaggcta cccgcaatgc
     2041 caatcaccgg tttatggtac ttcttcgcca cgtttgcgac accaatcggt accttgccgt
     2101 gaatactctg gctgtcaata cgcccttcac cggtgatcac cagcgtacaa tcgtgaatat
     2161 gttcttccag attcagcacc gtagtgacga tttcaatacc acttttcagt tccgcaccaa
     2221 gaaacgccat cagcgccgcg cccataccgc ccgcagctcc tgcgccgggg acatctttca
     2281 catcaacatg cagcgctttt ttaatgacct cggcatagtg agagaggtta ttgtccagct
     2341 caacaatcat cgcttcactg gctccctttt gtgggccaaa gatgcgcgat gcgccgttat
     2401 cgcccaccag cggattggtg acatcacaag cgacgcgaat gacgcaatct tttaagcgcg
     2461 gatcgaggcc ggaaatatca atatcattca gagtattaag actaccgccg ccaaaaccaa
     2521 tttcattgcc gttggcgtcg cataatttcg cccccagcgc ctgtaccatg cctgcgccgc
     2581 catcatttgt agcgctgccg ccaatgccga taataatgtt tgtcgcaccg ctctccagcg
     2641 cctgcaggat taactcgcct gtgccgcgtg aagtggtcac gagtggatcg cgtttttccg
     2701 caggtaccag ctccagccca ctggccgccg ccatttcaat aaacgcggtt ttgccatcgc
     2761 cggagatccc ccaactggca ttcactttct cgcccaacgg ccctgtaacc caggcgtgac
     2821 gttcggaacc ctgggtggct gcaatcatcg cttccaccgt tccttcgcca ccgtcggcaa
     2881 ccggaacaga aacgtactgt gcatcaggaa aaatttcccg aaatcctttt tctatcgcct
     2941 gcgcaacctc gctggcagat aaactttctt tataagagtc tggggcgatt acgattttca
     3001 tacctatgcc tgttaccaca tgacgccgga gggcgtttct cttattcggc gtggattcca
     3061 ggcccggatt gcaatacgcc atccgggcac gacgtcatta acgagtaact tcgactttcg
     3121 ccagtttttc gtagtagcac gccagagcgc tatgatccgc cgttcctaaa ccatctgcac
     3181 gcagtgcctg catcatctcc ataaccgcag ctgtgagcgg cagttgcgcg ccgacgccgt
     3241 gagaagtatc cagcgcattc gccagatcct taatatgcag atcaatgcgg aagcccggct
     3301 tgaagttgcg gtccatcacc atcggcgctt tggcatccag cacggtactg cccgccagcc
     3361 caccgcgaat tgcctgataa accaggtccg ggttaacgcc cgctttagtt gccagcgtta
     3421 acgcttctga catcgcagca atattcagcg ccacaatgac ctgatttgcc agtttggtga
     3481 cgttacctgc accgatttcc ccggtatgca ccacggaacc cgccatcgct ttcatcaaat
     3541 catagtattt gtcgaaaata gccttgtcgc cgcccaccat cactgacagc gtaccgtcga
     3601 tggctttcgg ttcaccgccg ctcaccggag catccagcat atcaatgcct ttcgctttca
     3661 gcgcttcgct gatttcacgg cttgccagcg gtgcgataga actcatatcg atcaataccg
     3721 tacctggctt cgcgccttca ataatgccat tctcacccag cgccacctct ttcacatgag
     3781 gggagtttgg cagcatggtt atgatgacgt cgcactgttc agcgatcgct ttagccgtag
     3841 acgctgtttc tgcacctgca gcaatcacgt cagcaatagc ttctgggtta cggtcagcaa
     3901 ccaccagcga gtaacctgct ttcagaaggt ttttactcat tggtttaccc ataatcccca
     3961 ggccaataaa accaactttc atagtcatat caatcatctc tcttgttgcg gtggtggtta
     4021 ttttttaaag gtatcagcca gtttctgagt ggcagagcgg aagacgccaa gatcgctgcc
     4081 gacagccaca aacgtcgcgc cccattccag ataacgacgc gcatcggctt cgaccggcgc
     4141 gagaataccg ctgggtttgc cgtgcgcact ggcacggtta aaaatatgct gaattgcttt
     4201 ttgtacatcc ggatgcgagg cattgccgag atggcccaat gccgcagcca gatcgctggg
     4261 gccgacgaag ataccgtcta caccttcggt agcggcaata gcatcgacgt tatctacgcc
     4321 ctgctgactt tctatctgga ccagaatagt gatgttcttg ttcgactgag cgaaataatc
     4381 cgccacggtg ccaaacatat tggcgcggtg agaaacggag acgccgcgaa tgccttccgg
     4441 cgggtaacga gttgatgcca ccgcctgctc tgcttcctct tttgtttcta caaaaggaat
     4501 caggaagtta tagaaaccga tatccagaag acgcttaata attaccggct cgttggtcgg
     4561 cactcgcact actggcgcgc tggcgctgcc tttcagggcc attaactgcg gaataaacgt
     4621 ggaaatatcg tttggcgcat gttcgccatc cagcaccagc cagtcaaacc cagccaaacc
     4681 aagaacttca gtgctaatcg ggttagagag tgctgaccag caaccaattt gtacctgttt
     4741 cgcagccagt gcggctttga atttattcgg gaaaacatcg ttattcatcg cttatacctt
     4801 tgcttatttc tgcaattcca tacgtttaat gtcgccaact acgaagaggt agcagaccat
     4861 cgccatcagc gctgaacatc ccacgaaaac cagtgctgca ttgaaggagt gcagttcact
     4921 taccaggtag ccaatcacca gtggagtgac aatggaggca acattgccaa agacgttaaa
     4981 gacgccgcca cagaggccaa caatctcttt cggcgcggtg tcagaaatca ccggccagcc
     5041 cagagcacca aatcctttgc caaaaaaagc cagcgccatc agcatgacca ccagcgtggt
     5101 gttgttggtg tagttacata agatgatggt ggaagccagc aacattccca gcacaatcgg
     5161 tagcttacgt gccagggtca gggataaacc gcgtttgatc agataatccg agaagacacc
     5221 tcccagcacg ccgcccgcaa aaccacacag tgccggaatc gaggcgacca gacccacttt
     5281 cagaatcgac atgccttttt cctgcaccag ataaatcggg aaccaggtga ggaagaacca
     5341 ggtgatggtg ttgataaaat attgtccgaa aaatacgccc aacatcatgc ggttagagag
     5401 caattgcttg atgtaatgca gtttgggacc gcttgctgcc gcgctgcccg gctttttgtg
     5461 gtccatatcg accaccgcgc cattttcaga gataaacttc agctcttccg cagacatacg
     5521 tgggtgatct gtcgggttat aaatcaactt gatccacagc gccgtcagca caaaaccaat
     5581 cacccccata acggtgaaga cgtgctccca gccccaggcg aaagtcagcc agccaagcag
     5641 aggcgaaaag agcgccagcg agaaatattg cgccgagtta aagatggcgg aggcagtacc
     5701 acgttctttc gtcgggaacc aggcggcgac aatacgggcg ttcgccggga atgatgg
//
LOCUS       DAJTYD010000202         5754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTYD010000202 DAJTYD010000000
VERSION     DAJTYD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.204"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(623..2332)
                     /locus_tag="OO585_003590"
     CDS             complement(623..2332)
                     /locus_tag="OO585_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6631041.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="OO585_003591"
     CDS             complement(2764..3483)
                     /locus_tag="OO585_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6631042.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3608..4171)
                     /locus_tag="OO585_003592"
                     /pseudo
     CDS             complement(3608..4171)
                     /locus_tag="OO585_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4155..4413
                     /locus_tag="OO585_003593"
                     /pseudo
     CDS             4155..4413
                     /locus_tag="OO585_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4522..5100)
                     /locus_tag="OO585_003594"
     CDS             complement(4522..5100)
                     /locus_tag="OO585_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631043.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKDLYKCV"
     gene            complement(5521..5754)
                     /locus_tag="OO585_003595"
                     /pseudo
     CDS             complement(5521..5754)
                     /locus_tag="OO585_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1606 a         1253 c         1040 g         1855 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccatac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtag
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac ggtcaggaat caggctgcat acgctgtgcc
     2701 aaatctaatc gtagttattc taaccagttt tccagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt gcggtagccc ttggctttta ttctcagcag cctgatcttg ctgttcagtg
     3721 cttccgcatt tccgtttgat acgccatggc gcatggcgtt caggataccg tacaaccttt
     3781 tccctatcgt tttagccaca ttcttcatca tggggacgtc actgttggcc gccagcgaca
     3841 gccatctctg ccagtcgttg cgtctttcct cgctccatgg tctgctccag atatcctttg
     3901 ccagttcttt cagcgcccag cacaggctcg tcagcttcat ctgcgcacgc agccatatca
     3961 gcttttcctg cctggattcg gtcatccatt tatcgctgtg ctgccacagg aagcgagttc
     4021 cctttgcctg acgacgactt tcaacaggaa gatgcggatg ttcattctgg cgggttttat
     4081 ccaccacctc acccggttgt ttcgccacat gaaagcgatc gaaggctatt ttctcaaccg
     4141 cattgggtaa gtggatgcgc gctgctctta gtttgctgag ccagtttagc attgccttct
     4201 acatgaccga tgcctgagcc ggtataccga actttattgt cctcaaccag tcatgttata
     4261 agcaagaaat gcactcagcg gatagagcga cataatctga acctgcggac acatcttaag
     4321 cggcttaccc gcaaaaccat atgcttctca aagtctgatg atatgcacta taagatcatc
     4381 ggctggtatc tgacaatcaa ccaccaccac taaatctgcg tcacgacctg caactgagtc
     4441 tggttgaaca ggctgaggcc ctgctgaagc aacgcgaacc tctcgactct tgttggattt
     4501 taccaaatag tttttctaaa attaaacaca tttatataag tcctttgctg aacaaaacgc
     4561 agcccgccat cctttttcat tcataaattc agcaatggca gataaaactt tttcatcaag
     4621 catataaaaa ataatgcaag tagactcaat atgcctcata caaataaggc cgcacatatc
     4681 cgtaatatat ttgtcaatgc tgctataatt tgcatggtaa tatgccatgc tattatctga
     4741 tgattttaca tatgcagtat gacatatttt tccttcacga ttaacaaaac tgacaaaacc
     4801 actttcacca ggagtaatat ataaagatga atttaacgtg cgctcactaa tattcattaa
     4861 ttgttgaaat tcaatggaat agttttcagt taatgtatct attatcttgg ctttgtcttt
     4921 ctttgttatc tcattgtctt tttcggcaag aaaaattgcc actttatgac atgcattcag
     4981 ctcgttaatt ttacctgata aaactgcttt tcttaccaca tcgttgaact catcgatagt
     5041 gtgtttttta ttatatatat catgaaaaat atagcctaaa gacttaaacg agactttcat
     5101 tttatattct ccttaaatat taacaggata tatatatcac attttcctct atattttcag
     5161 aaaccagcta tcattttccc cattactggt acgtcgcttc agatatttcc gcagcaagcg
     5221 ataaccctgc gcactatgag gatgccattc attataatgt ttgaacacct tcgcgagact
     5281 actttactgc tgttaacacg tcaggttcga acataacact gatttaatca tatttcatcg
     5341 ttttgacgaa gttttctgcc attccatttt gcaccgccgt gtgtttgggg gcaaggccga
     5401 ccatctgtgt ttcattcgcc agatataagt gatgagatgg cattctccct ccttcacggc
     5461 agaagcattt tgaccgttta ggggatgcgg acgattttca tgcctgctaa gatactttcg
     5521 ttactgatgc tgtccgtttt ccaccatttc atcatcactg attggcgttg tgtttttggt
     5581 cagcactcca gctttacgtt tctcttttaa ccggtaactc tctcctttga tattcagcgt
     5641 gtagcagccg atctaggatc gctgttgcca gcacgtgatc tccgaacatt tctccccagt
     5701 ctgcgacccc tttgtttgac gtcagtatga tgctcgcttt ttcatatcga cagt
//
LOCUS       DAJTYD010000203         5744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTYD010000203 DAJTYD010000000
VERSION     DAJTYD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.205"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..382)
                     /locus_tag="OO585_003596"
                     /pseudo
     CDS             complement(1..382)
                     /locus_tag="OO585_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(568..3876)
                     /gene="icsA"
                     /locus_tag="OO585_003597"
                     /gene_synonym="virG"
     CDS             complement(568..3876)
                     /gene="icsA"
                     /locus_tag="OO585_003597"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6631044.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4405..5607
                     /gene="virA"
                     /locus_tag="OO585_003598"
     CDS             4405..5607
                     /gene="virA"
                     /locus_tag="OO585_003598"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6631045.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1777 a         1298 c          949 g         1720 t
ORIGIN      
        1 gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta ggatcttcac
       61 cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg ttactaattt
      121 atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct acttggaaat
      181 gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt acagaactac
      241 tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt gctcgacctt
      301 gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga ggcgcataac
      361 cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct gtgtaacgcc
      421 aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga tcgcaggcat
      481 accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga gaaatgcagg
      541 acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca aaataccttg
      601 ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga tgcttaggtt
      661 atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt tgcctatctg
      721 gtgatttgac atgccattca taattacacc atattgcttt gagctccatt tccaattcac
      781 ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactat gactaattaa
      841 ggatgtacgc aatcccaatt ttgcttgaat attattgacc ccagaggatg tgaccatagt
      901 gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct ggacttgtgg
      961 attgagatac aatgcgttgt caatattatt atgagtagtc cagcgtatgg ttgggtagat
     1021 atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac cagttaaccc
     1081 atctcctttg actgtattat taaaccaacc gtactgcaac caagcatcca tatatagacc
     1141 tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat aaccgctcac
     1201 agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct gatatccata
     1261 agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct tcaaaatatc
     1321 tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat cagctaaatt
     1381 atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga gctgacctgt
     1441 agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa acatcgtgtt
     1501 cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt gaatactacc
     1561 ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac tcatttgagt
     1621 agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag ttagatacca
     1681 cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat aactaccagc
     1741 aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg aaataatttg
     1801 aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa cataggtaat
     1861 accagacgca tggcctgtaa tttccagccg gtcagtttta ctgttgtcat tgccgagaac
     1921 cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa cataggtctg
     1981 accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca gagtcatgtt
     2041 acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga ttagcacagt
     2101 cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca aggtagcacc
     2161 tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca tttttagaat
     2221 gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa tgagagttcc
     2281 ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat ggctgagatt
     2341 atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga actctccatg
     2401 actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct ggtcactgat
     2461 atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga tagatatatt
     2521 agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt ccttctgcgc
     2581 ccagttattt ataatagctt ccacttttac attgttgcca aatgacatat ttccagaagc
     2641 ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag aaacgtcacc
     2701 tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag cacataaatc
     2761 accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca gaatggatga
     2821 attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta atgaattgtt
     2881 tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat cttctttacc
     2941 tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag catcccccca
     3001 atgtccacca ttaccgccta aaatagctcc attgttgatt atagacaggt tatctcctgt
     3061 gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc cgttgcctcc
     3121 agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat cacctccatt
     3181 accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag tccctttgtt
     3241 gattatgggt agattgactc ctgtgacggc atcacctcca ttccccccat cactatcact
     3301 ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa acagattgtc
     3361 tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc cgctaccacc
     3421 aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt caccaccatt
     3481 accgccacaa ctattaccat cattattatc accagcacca ccatgacctg cgctaatcat
     3541 aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg aagaagttat
     3601 ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat caacaggtgt
     3661 taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg aaagaggaat
     3721 agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag gagaaaaaga
     3781 caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct gtgaacattg
     3841 ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca gtaagtggtt
     3901 gataaacccc taaaaagatt ccgttttatc cggaataaag ggacgatata tgcaaaaaca
     3961 tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg caacgggaga
     4021 aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc gataataaat
     4081 taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt tctgtacgct
     4141 tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct gccccttgat
     4201 gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct accagactct
     4261 ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt aaagagaaga
     4321 ataacattcc attttattat gttcccaatt taattatatt aatatatatt ggcatcaata
     4381 ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca tgaaagaaat
     4441 gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa taaattgagt
     4501 ttttgtgacg ttttactgaa aataataact ttcggaatat attctccaca cgaaactcta
     4561 gccgaaaaat atagcgaaaa aaaactaatg gatagtttct caccatcact atcacaagat
     4621 aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg gctatgcctt
     4681 aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc cactcaactt
     4741 tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt caacttagga
     4801 aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa gcctgcacca
     4861 gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga tgattacttt
     4921 cccgttaaag acactgattt taatagtgtt tggcatgata tttacagaga tattcgagca
     4981 tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact aaaattaatt
     5041 gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa agagcttcaa
     5101 atgctaagtt acaaccaatt aaataaaata atcaattcaa acttccccca acaagaccta
     5161 tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc atttatatcc
     5221 gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt ggaacacata
     5281 tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc tgaacaacga
     5341 gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa aaacgacact
     5401 gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt accatcgcac
     5461 ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt tcatgctgga
     5521 acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg tcaagtatca
     5581 tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag actgtaaaaa
     5641 aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat ggggaccaat
     5701 agaatctcag cccatccttt ttatatcatt atgaaccgcc ccgg
//
LOCUS       DAJTYD010000204         5708 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTYD010000204 DAJTYD010000000
VERSION     DAJTYD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.206"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..457
                     /locus_tag="OO585_003599"
     CDS             <1..457
                     /locus_tag="OO585_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631046.1"
                     /translation="NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLP
                     AIKLGTTCRRAGDGLKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRK
                     NRLPVEVVKIPLAGPLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            454..855
                     /locus_tag="OO585_003600"
     CDS             454..855
                     /locus_tag="OO585_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631047.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            866..1606
                     /locus_tag="OO585_003601"
     CDS             866..1606
                     /locus_tag="OO585_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6631048.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1665..2051
                     /locus_tag="OO585_003602"
                     /pseudo
     CDS             1665..2051
                     /locus_tag="OO585_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
     gene            2060..2388
                     /locus_tag="OO585_003603"
                     /pseudo
     CDS             2060..2388
                     /locus_tag="OO585_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2360..5401
                     /locus_tag="OO585_003604"
     CDS             2360..5401
                     /locus_tag="OO585_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6631049.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5401..>5708
                     /locus_tag="OO585_003605"
     CDS             5401..>5708
                     /locus_tag="OO585_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631050.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     "
BASE COUNT         1391 a         1310 c         1851 g         1156 t
ORIGIN      
        1 gaataccgtt gcgggagata accaggtgaa ggggggcccc ctgaaactgg tgcgtcagcg
       61 cgtccgggta tttaaagcca gtccggcagg aaaaatgtat gcccgtatcc gtgttaaccg
      121 gggcaatctg cccgccatta agcttggcac aacatgccgg cgtgccggtg acggactgaa
      181 ggtggggaaa tacttttttc gtggtgcgtt tatccagcag ctggcgaacg gtcgctggca
      241 tgttctcagg cgtcttcctg aagcgtgttt tgaaacagga aaggacagta aggggcggcc
      301 cagaaaaaat cgtctgcccg tggaggttgt gaaaattccg ctggccggac cgctgacaca
      361 ggcatttgag gatgcccgcg accgcatcat tgccgcggaa atgccgaaac agctggggta
      421 tgccctgaaa cagcaactga ggatacattt gggatgaaca ggcacacaca aatccgccag
      481 gcagtactgg caagcctgaa gggcgaatgc ggggggaatg tcgtgctttt tgacgggctt
      541 ccggcattta ttgatgcgca ggaactgccc gccgtggcgg tgtggctgag tgatgcgcag
      601 tacaccggaa aaatgacgga tgaggacgac tggcaggctg ttctgcatat tgccgttttc
      661 gtccgtgcac aggcgccgga ttctgagctg gatacgtgga tggagaacac catatttccg
      721 gccatgaagg atattccggt gctttccgga ctcatcgaca ccatgatccc actcggtttt
      781 aactatcaac gtgataatga gatggccacc tgggcgatgg cggaaatcac gtaccagatc
      841 acgtacacga attaaggagg tggcaatgac cacaccaaat ccactggcaa agacgaaagg
      901 tgcgggaacg acgttctgga tgtacaccgg caagggcgat gcgtttgcga accctttgtc
      961 ggacactgac tggctgcgtc ttgcgatggt gaaggatctg caacctggcg aaatgaccgc
     1021 tgatgcagaa gatgacactt atctcgatga tgaagatgca gactggaaaa cgacaaccca
     1081 ggggcagaaa tccgtcggtg atactttggc gacgctggcc tggcgtccgg gtgacagcgg
     1141 gcagaaaaaa ctggttcagt tgttcgattc cggtgaagtc tgcgcgtttc gtatcaaata
     1201 ccccaacggt acggttgatg ttttccgtgg ctggctgagt tcactgggta aaaccattac
     1261 ctcaaaagag gtgatgaccc gtactgtaaa aatcagcggt gtggggcgtc cgtatctggc
     1321 agaggagggc gctgaaaccg tgggtgttac cggactgacg gtggcaccgg catctgtcag
     1381 tgtcaaagcg ggagcaacca ccacactgac ctttacagta aaacctgacg gggccagtga
     1441 caaagcgatc agtgtgcatt cgtcagatcc tcagactgct tcggtgaccc tgagcgggct
     1501 tgtggccacg gtgaaaggcg tgaagcaggg cagtgtcagc atcgtgggca tgacctctga
     1561 cggagagttt gtggcagtgg ctgcggtgac cgtcagcgca ccataacagg acgatactca
     1621 tcattgcccc ggttatccgg ggctttttgc atccggagaa catgatgttt ctgaaacagg
     1681 atacgtttaa ttatgaaaaa cagtccgtgg tgctcagtga gctgtccggg ctgcagagaa
     1741 ttgaatatct gacgtttgtt cagcagcgaa cggcaaagtt tgatgcacag gagggagaac
     1801 tgccggaggc tgaacgacag attgcttttc tgcgtatggg aatggatatc aatgcctggc
     1861 tggtttcccg ctcactgtgg aatgctgagc agtctcagga tgttgagacg ctttgagcat
     1921 ccattatgac aacatggtcg tatgatgcgc tgggcgcggg ggcggagagg gttctgtcgc
     1981 tgagcggtat ggggaccatt gagaatgccg gggatgatga tcatgaggcg ctgacgccgg
     2041 aaaagtcctg acgcgggaaa tgcagtttgt catgcggctt gcccgggagt tccggcgggc
     2101 agactggcgg cggatgctgt cggaaatgtc ggccactgag cttggtgagg gggcgattat
     2161 ttccggatgc agagcttcag tgatgtgtgg atggatgcgc agtttgcctc gctgaaggca
     2221 ttgatcgtga gaatggtgtc cggcagcagt gatgctgcgg tggctgattt cagcctttta
     2281 ccggaagaga acgggatacc ggagcgaacg gacgaagaac tgatgcatct tggggaaggt
     2341 atttccggag gtgtgcgtta tggaccagat agccaacctg gtcattgatt tggggattga
     2401 tgcggcagag tttaaaaatg aaattccccg tatcaaaaac cttctgaatg gtgcagccgg
     2461 tgatgcagaa cggtcttctg cccgtatgca gcgttttatg gagcgtcaga ctcaggcggc
     2521 ccggcagaca atgcatgcgg cttcttcggc tgcaacagca gcatcagccc atgcgcagac
     2581 ggtggagaag aatgcacggg ctcatgaacg catggcccgt gaggtggaac aaacccgtct
     2641 gcgcgtggat gccctgaatc agaaaatgcg cgaggaacag gcgcaggcca gggcactggc
     2701 ggaggcgcag gataaagcgg ctgccgcctt ttatcgccag attgacagtg tgaaacaggc
     2761 cggtgcgggg cttcaggaat tacagcgtat tcagcagcag atccgacagg ccagaaacag
     2821 tggcggggtt ggtcagcagg attatctggc gctgatttcg gagatcacgg cgaaaacccg
     2881 tgccctgacg caggcagagg aacaggccac ccggcagaaa gcggcgttta tccgccagct
     2941 taaagagcag gcaacccgcc agaatctgtc gtcttctgag ttgcttcgcg ccagggcggc
     3001 tcagctgggg gtaagcagtg ctgcagaagt gtatatccgc aaaatggagc gggcaggaaa
     3061 agccacacat tcgctggggc tgaaaagtgc ggcagcccgg cgggagctgg gggtgttaat
     3121 cagtcagatg gcgcgcggca attttggtgc gctgagggga tccgggataa cgctggctaa
     3181 ccgtgccgga tggacaggcg cactgatgtc gccgaaaggc atgatgactg gcggcgttat
     3241 tggcggactt gtcgcggcgg tcctgggtct gggtaaagcc tggcatgacg gccggaagga
     3301 gggcgaggaa tttaaccgtc agctggcgct gacgggacat tatgccggtg tcactgtcgg
     3361 gcagttgtgg aaactcagcc gggccatatc cgggaatggt atcacgcaac atgcggcagc
     3421 cggtgcgctg gcacaggtgg tgggcagtgg ggcatttcat ggaaacgata ttggtatggt
     3481 ggcgaaagct gccgcacaga tggagcgatc ggttggccag tcggtcagcg ataccataaa
     3541 tcagtttaag cggctgaagg atgatcctgt aaatgccgcg aaggcgctgg acaatgcgct
     3601 gcattttttg actgccactc agcttgagca gatacgcgtc cttggggagc aggggcggtc
     3661 cagtgatgcg gcccggatcg ccatgtctgc gctggcagag gaaaccggta aacgcacatc
     3721 tgatattgat aataatctca atgcgctggg tagtacgctg caaaccttgt ctgactggtg
     3781 gaagcagttc tgggatgcgg ccatgaatat tggtcgtgaa gactcgctgg atgcgcagat
     3841 tgatgcgtta caggaaaaaa ttcagcgcgc gaaaaaatat ccgtggacaa acgcctccac
     3901 acaggtggag tacgatcagc agcgtcttaa cgatcttcag gagaaaaaac gccggaagga
     3961 tttgcaggat gcaaaagcgc aggcagaacg gaattaccag gagcaacaga aacgccggaa
     4021 tgctgaaaat gccgcgctga accggatgaa tgaaacggaa gctgcacgac atcagcggga
     4081 aattgcgcgt attaatgcca tgcagtacgc cgaccaggct gtcagggatg cggcgataca
     4141 gcgtgaaaat gaacgttacg aaaaagccat taagaaaaat acacgggcaa cccgtaatga
     4201 tgaggccacc cggttattgc tgcagtacag tcagcagcag gcacaggtgg aagggcagat
     4261 tgctgctgcc agacagtcag caggcattgc cactgaaagg atgactgaag cgcataaaca
     4321 gcttctggcc ctgcagcagc gcatcagcga cctggacggg aaaaagctga cggcagatga
     4381 aaagagtgtg ctggcccgta agaatgagct gattcaggcg ctgacgctgc tggatgtgaa
     4441 acagcaggag ctgcagaaac agacagcgct taacgacctg agaaaaaaaa cggttcagct
     4501 gaccagccag ctggcagaca aagaacgtgc actgcgtgag cagcacaatc tggatattgc
     4561 cactgcaggt atgggggata agcagcggca gcgctaccag gcacagttgc gcatccggca
     4621 ggaataccgg caacagttgc aacagcttga gaatgacagt cgccagaaag gcacttacgg
     4681 gacggaggac taccggaggg ctgaggaggt gctgaagggg agcctgaagc gacaactgaa
     4741 tgaaaacaaa cgctactggc aggaactgga agtggcgcag ggcgactgga aaaacggtgc
     4801 catgcgggcg tttcagaatt ttacggcgga tgcggataat gcggtgggaa cggcagaaca
     4861 gatgtttaca gtggcattca gcagtgccgg taatgcactg gcgacattct gtaccaccgg
     4921 taagctgaat tttaaatcct tcacctcttc cctgttgtca gatatggcca gaattatggc
     4981 acagatggcc atgatgcagg cggtaaaggg cgtcggctct ttattcggct tcacgactaa
     5041 tgctgatggc ggtgtttacc agtctgctga tttgagtcgc tacagtggca cggtggttaa
     5101 ccgtccgacg ttttttgctt ttgcaaaagg cgcgggtgtg atgggggaag cgggacctga
     5161 agccattctg ccactgcgtc gcggtgctga tggtaagctg ggggttgtgg cggatactgg
     5221 tggttcaggc atggtgatgt ttgccccgca gtacaacatc gggatcaata acgacggcac
     5281 gaacgggcag ataggtccgg cagcactgaa ggtggtttat gacctcggga aaaaagcggc
     5341 cgcggacttt atgcaacagc aggcccgtga tggtggccgg ttaagtggag cataccggta
     5401 atggagacgt ttcgctggaa ggtgcgcccg gatatgaatg tggtatcaga gccaaaagtg
     5461 gtggcagtga agctgggtga tggttatgaa cagcgtcgcc cggcgggact gaataatctg
     5521 ctgtcgactt acagcgtgac gatacgtgtt cgtaaaggtg aacacccacc tttaaaagcc
     5581 tttctggaac ggcacggtgg cgtccgcgca tttcagtgga cgccacctta tgactggaaa
     5641 ccgatcaggg tggcttgtcg taaatggtcg gcaagcgtgg gggcgttatg ggtgactgta
     5701 acggccga
//
LOCUS       DAJTYD010000205         5639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTYD010000205 DAJTYD010000000
VERSION     DAJTYD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.207"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            21..143
                     /locus_tag="OO585_003606"
     CDS             21..143
                     /locus_tag="OO585_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR6631051.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            159..1004
                     /locus_tag="OO585_003607"
     CDS             159..1004
                     /locus_tag="OO585_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6631052.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1027..2538
                     /locus_tag="OO585_003608"
     CDS             1027..2538
                     /locus_tag="OO585_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6631053.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGGRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2563..3561
                     /locus_tag="OO585_003609"
     CDS             2563..3561
                     /locus_tag="OO585_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6631054.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3594..4688
                     /locus_tag="OO585_003610"
     CDS             3594..4688
                     /locus_tag="OO585_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6631055.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4700..5572
                     /locus_tag="OO585_003611"
     CDS             4700..5572
                     /locus_tag="OO585_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6631056.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
BASE COUNT         1265 a         1323 c         1688 g         1363 t
ORIGIN      
        1 aaaaccctac aggaggcact atgcctaaaa aaatgagaac aacccgtaat ctattattaa
       61 tggctacgct attaggtagc gcactatttg ccagggctgc ggataaagaa atgaccattg
      121 gtgcaatata ccttgatacc tagggatatt acgctggagt gcgtcagggc gttcaggatg
      181 cggcaaaaga ttcttcagta caggtacagt taattgaaac taacgcccag ggtgatattt
      241 cgaaagaaag tacctttgtt gataccctcg tggcgcgtaa tgtcgatgcc attattttat
      301 cggcagtgtc tgaaaatggc agtagccgta ccgttcgtcg cgccagtgaa gcgggtattc
      361 cggtgatttg ctacaacacc tgtattaatc aaaagggtgt cgataaatat gtctcggcgt
      421 atctggtcgg cgatccactg gaatttggta aaaaactgag taacgctgcc gccgattatt
      481 ttattgccaa taaaattgac cagccgaaaa ttgccgtcat caattgcgaa gcctttgaag
      541 tttgtgtgca gcgacgtaaa ggatttgaag aagtattaaa atcccgcgtt cccggcgcgc
      601 aaattgtcgc taatcaggaa gggactgttt tagataaagc gatttccgtt ggtgaaaaac
      661 tgattatctc cacgccggat ctcaacgcca ttatggggga gtcgggcggt gcgacactcg
      721 gcgcggtaaa agcggtacgt aatcaaaatc aggccggaaa aattgctgtt ttcggttcgg
      781 atatgacaac cgaaattgct caggagctgg aaaacaatca ggtgctgaaa gcggtagtgg
      841 atatttccgg taagaaaatg ggcaatgctg ttttcgcgca aacattgaag gttatcaata
      901 aacaagccga cggtgaaaaa gtgattcagg tgccaatcga tctctatacc aaaacggaag
      961 atggtaaaca gtggctggca acgcacgttg atggtctgcc ctaatcgctg attcgcagag
     1021 gtccctatgt tcacggcaac agaggcagtc ccggtagcaa aagtggtggc aggaaataag
     1081 cgttaccccg gcgtcgttgc gctggataac gttaacttca cgctcaataa aggcgaagtt
     1141 cgtgcgctgt taggtaaaaa cggcgcgggc aaatcgactc tcattcgaat gcttaccggc
     1201 agcgaacgcc cggatagcgg tgatatctgg attggcgaga cgcgactgga aggtgacgaa
     1261 gctacgttga ctcgccgtgc cgctgaactg gggggtcgtg cggtttatca ggaattaagt
     1321 ctggtggaag ggctgacggt ggcagaaaac ctctgccttg gtcaatggcc tcgccgcaac
     1381 ggcatgattg attacctgca aatggcgcag gatgcccaac gttgcttaca ggcgctgggc
     1441 gttgacgtta gtcctgaaca acttgtttca acgctaagcc cggcgcaaaa gcaactggtg
     1501 gaaattgcac gggtgatgaa gggcgagccg cgcgtggtca ttcttgatga acccaccagc
     1561 tcgcttgcca gtgcagaagt tgaactggtg atcagtgcgg tgaaaaagat gtcggcactg
     1621 ggcgtggcgg tgatttatgt cagccaccgg atggaagaaa tccgccgcat tgcctcctgt
     1681 gccaccgtta tgcgcgatgg tcaggtggcg ggcgatgtga tgctcgaaaa cacctccacg
     1741 catcatattg tgtcgctaat gctagggcgt gatcacgttg atattgcgcc ggttgcccct
     1801 caggaaattg tggatcaggc cgtactggaa gtccgtgcgt tacgccataa gcccaagctg
     1861 gaggatatca gctttacgtt acgtcgcggc gaagtgcttg gcattgctgg cctgctgggg
     1921 gcagggcgca gtgaattgct gaaggcgatt gttgggctgg agacgtatga acagggcgaa
     1981 attgttatca acggcgagaa aatcacgcgc cccgattacg gcgacatgct gaaacgcggc
     2041 attggctata cgccagaaaa ccgcaaagaa gcggggatca ttccctggtt gggcgttgac
     2101 gaaaatacag tgctgaccaa tcggcaaaaa atcagcgcca acggagtgct gcaatggtcc
     2161 accatccgcc gcctgaccga agaggtgatg cagcggatga cggtcaaggc cgccagtagc
     2221 gaaacgccca tcggcacgct ttctggtggc aatcagcaaa aagtggtgat cggtcgttgg
     2281 gtctatgccg ccagccagat tttgttgctc gacgagccaa cgcgcggcgt cgatatcgaa
     2341 gccaaacagc agatttaccg tattgtccgt gagctggctg ccgaaggaaa aagcgtggtg
     2401 tttatctcca gtgaagtgga ggagttgccg ctggtgtgcg accgcatcct gttgttacag
     2461 cacggtacgt tctcgcagga gtttcactcg ccggtcaatg tggatgagct gatgtccgcc
     2521 attctgtctg tgcactgatg aattttaccg aggaacctga agatgtctgc ttcgtcatta
     2581 tcattgccgc tgggcaagag cgtctcgctc aaacaatttg tcagtcgcca tattaatgag
     2641 atcggtttgc tggtggtaat cgccattctt tatctggtct tctccctgaa tgcgcctggc
     2701 tttatctcat tgaataacca gatgaacgtg cttcgcgatg ctgccaccat cgggatcgcc
     2761 gcctgggcaa tgacgctgat tattatctcc ggtgaaattg atgtcagcgt tggaccgatg
     2821 gtggcttttg tctcggtgtg cctggcattt ttgctgcaat ttgaagttcc gttggcgatt
     2881 gcttgtctgc tggtgttgct gttaggcgcg ctgatgggaa cgctcgccgg ggtgctgcgc
     2941 ggcgtgttta acgtgcccag tttcgttgcc acactggggc tgtggagcgc cctgcgcgga
     3001 atggggctgt ttatgaccaa cgcactgcca gtgccgattg acgaaaacga ggtgctggac
     3061 tggctgggcg gacaatttct cggtgtgcca gtatccgcgc tgatcatgat ggtgttgttt
     3121 gcgctgtttg tgttcattag ccgaaaaacc gccttcgggc gctcggtttt tgccgttggc
     3181 ggtaatgcca cggcggcgca gttgtgcggt attaacgttc gtcgagtgcg cattcttatc
     3241 tttacccttt cgggattatt agcggcggtg accggcattt tgctggcggc acgcctcggt
     3301 tccggtaacg caggtgccgc aaacggtctg gagtttgacg tcatcgccgc ggtcgtggtc
     3361 ggcggtacgg cactttccgg cggtcgcggt tccctgttcg gtacattgct tggtgtgctg
     3421 gtgattacgc taatcggtaa cggtctggtg ctgctcggta ttaactcctt tttccagcag
     3481 gtggtacgcg gcgtcatcat cgtggtggcg gtgctggcga atatcttgct gacccagcga
     3541 agcagtaaag cgaaacgcta aaccttaacg cccctggcgc gggtgcggca gggatgaaaa
     3601 ttaatcgtat tgttaatgag ggatttatga aaacgatgct ggcagcttat ttaccaggaa
     3661 attcaaccgt cgatctgcgg gaagttgcgg tgccgacgcc ggggattaac caggtactga
     3721 tcaaaatgaa atcctccggg atttgcggaa gcgatgtcca ctatatctat catcaacacc
     3781 gtgccacggc ggcggcaccc gataaaccgt tataccaggg atttatcaac ggtcatgaac
     3841 catgcgggca gattgtggcg atggggcaag gctgtcgcca ttttaaagag ggtgatcgcg
     3901 tgctggtgta tcacatttct ggctgtggtt tttgcccgaa ctgccgccgc ggctttccta
     3961 tctcttgtac tggcaaagga aaagcggctt acggctggca gcgtgacggc ggtcatgccg
     4021 aatacttgct ggcggaagaa aaagatctga tcctcctgcc ggatgcgctt agctacgaag
     4081 atggtgcgtt tatcagttgc ggcgttggta cagcgtatga aggaattttg cgcggcgaag
     4141 tttccggcag tgataacgtg ctggtggtcg gtctggggcc agtcggcatg atggcgatga
     4201 tgctggcgaa aggtcgcggt gcaaaacgga tcatcggcgt tgatatgctg ccggaacgtc
     4261 tggcgatggc aaaacagtta ggggtgatgg atcacggcta tttagcaacc accgaaggtc
     4321 tgccgcagat tatcgccgaa ctcacccacg gtggcgcgga tgttgcgctc gattgttctg
     4381 gtaatgccgc aggtcgcttg ctggcactgc gatccaccgc tgattgggga cgggtggttt
     4441 acatcggaga aaccggaaaa gtggagttcg aggtcagcgc cgatctgatg caccatcaac
     4501 ggcggattat cggctcctgg gtgaccagtc tgttccatat ggaaaaatgc gcccatgatc
     4561 tgacggactg gaaactgtgg ccgcgtaacg ccattaccca tcgcttctcg ctggaacagg
     4621 ctggagatgc ctatgcgctg atggcgagcg gcaaatgcgg gaaagttgtg attaacttcc
     4681 cggattaagg agttagcgga tgaccatcta taccttatct cacggaccgc taaagctgga
     4741 tgtctctgac cagggcggcg taatcgaagg cttctggcgt gacacgacgc cgcttttacg
     4801 tcctggtaaa aaaagcggtg tggcaaccga tgcttcctgt tttccgctgg tgccgtttgc
     4861 aaaccgggtg agcggcaatc gttttgtctg gcaggggcgt gagtatcaac tgcaaccgaa
     4921 tgttgagtgg gatgcgcact atctacacgg cgatggctgg ctgggcgagt ggcaatgtgt
     4981 ctcgcacagc gatgatagct tgtgtctggt gtatgaacat cgcagcggtg tctatcacta
     5041 tcgggtaagt caggcgtttc atttaacggc ggatacgctg acggtgacgc tctctgtcac
     5101 caatcaaggg gcagagacgc tgccatttgg taccggctgg catccttatt ttccgttgtc
     5161 accacaaacg cggattcagg cgcaggcgag cggttactgg ctggagcggg agcagtggct
     5221 ggcgggtgag ttttgcgagc agctaccgca ggaactggat tttaaccagc tcgcgccgtt
     5281 gccgcgccag tgggtgaaca atggttttgc cggatggaat ggtcaggccc gcatcgagca
     5341 gccgcaggaa ggttatgcca tcatcatgga aacgacgcca cctgcaccgt gttatttcat
     5401 ctttgtttcc gaccctgcgt ttgataaagg atatgcgttt gatttcttct gtctggaacc
     5461 gatgagccat gcgccggatg atcatcatcg cccggaaggg ggtgatctca ttgcgctagc
     5521 accaggggaa tcaacaactt cagagatgtc gttgcgggta gagtggctgt aatttattaa
     5581 atgccggata aggcgttcac gccgcatccg gcatctttcc taacgccgat cgagagaaa
//
LOCUS       DAJTYD010000206         5624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTYD010000206 DAJTYD010000000
VERSION     DAJTYD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.208"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1926)
                     /locus_tag="OO585_003612"
     CDS             complement(<1..1926)
                     /locus_tag="OO585_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6631057.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPSDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAVGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFT"
     gene            2168..3229
                     /locus_tag="OO585_003613"
     CDS             2168..3229
                     /locus_tag="OO585_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6631058.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3342..4841)
                     /gene="ansP"
                     /locus_tag="OO585_003614"
     CDS             complement(3342..4841)
                     /gene="ansP"
                     /locus_tag="OO585_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6631059.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5108..>5624
                     /locus_tag="OO585_003615"
     CDS             5108..>5624
                     /locus_tag="OO585_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR6631060.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLTLENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGH"
BASE COUNT         1467 a         1456 c         1367 g         1334 t
ORIGIN      
        1 ggtgaataag ccgacgagag tataagacgg cgcttttgcc gtattttcat catctgccat
       61 aatatcgccc atataacgcg cttctgtgcc tgcataccaa ccatcttccg gtacataacc
      121 tatcgacgca aagcccatat tacgggcgat ccctggcatc cgattaccgt tacaatcctg
      181 ttcattgcaa acattgctgc gataggtcgc atcaagccag gtccaggacg cgtttacgcg
      241 aaaatctcct gcgaaacgtt gatcccatgc cagttcagcg ccttgacgac gggtctttcc
      301 ggcatttttg taagtcgtac gcccaccgct actgctatcg acaacaattt catcatcagt
      361 gtcggtctga aacaatgcga gactaagcag cccatcacca atacgcgttt tactgccgat
      421 ctcaattgta tcgttggtgg atggttttaa accaaagttc ataccgcttt gcccatcagc
      481 acgataagac agctcattaa tcgtcggcgt ttcaaaacct cgcccgactg ccagatagat
      541 attccaggca tcggtcattg catattttaa cgaaccggca ggtagccatt tatgataact
      601 ggcatcaccg ctgtcatcgc cgttacccgg agtaacgtaa tggtcgttgg aatcaaacca
      661 cacggagctg tagcgcacgc cagcatccag cgacagtttt tcgctcagct gccactgcgt
      721 ctgtaaatag ggatcgatgt tccacatcag attgcgttcg tcgcgacgca actcaccttt
      781 ttgcccgtac tccggcatgc cgctattcag gcggaagtta ttgtagccct tgcggttttc
      841 actcatgttt tcgtagttca ggccggtagt gaacgtgacc ggaacgccca gttcaccacg
      901 gtgtgtccag cggctgtcta tcccctggta atggcgttgc agggtaatca cgccgcccgc
      961 atgtgacggg ttaagttgtg gtgccatggg tattgactgg tactgggtcg tttctcgctc
     1021 tccggcatac atcatcacac tcatatcatc ttgcgcgctc aggctacgct catagcgcaa
     1081 ccctgcctga gtttgcttga tggtttttcg cgtgtcgtac tgttctgcac gaggcgcttg
     1141 ttgtggatta gccttccatt ctgctttggt tagcccacct gggtcatctg ctttgatatc
     1201 cacactattg aaaatcagac ttaatttgct ggcttcatca atgcgtacgc ccagtttggc
     1261 attggctaaa tttttctgtg cgccactatg gtcacgatag ccgtgggtcg taaaacgcgt
     1321 ggttgagacg gtgtaatcga catcgctagg ctgtgtgccg tctcccgttg cgcccgttgc
     1381 tttcagccca tagcgccagc tgccaaaact gccgtagtaa ctactggctt caatggttgg
     1441 tggctgttgt ccggtctggg tggtgacatt cattacccca ccagacgcgt tgccatacag
     1501 ggcagagaag gggccacgca gcacttccac attttgcaca ctgcttaaat cgatgttgga
     1561 tgtttgccct tgcccgtcgg gcatggtggc gggaataccg tccacataca ggcgaatacc
     1621 gcgaataccg taagtggagc gggagccaaa tccgcgaatc gacagctgta aatcttgcgc
     1681 atagttctgc cggttttgta cctgcaaacc aggcacgccg gtcagtgatt cggacaagtt
     1741 aatgcgcggt gttgccaggc gcatctcctc gccatccacc acgcttactg ctgctggggt
     1801 atccagttct gaaaccacct gcggtgcggc actgacaatc atagtctgtt catcagcggc
     1861 aaaaacaacg ggggaaagga caagcagtgc gggcaaaacg gtctgtcgga cggaaaaaat
     1921 cttcatgaaa aaagccaggt taagaatggg aaaacgctgt catggtaata aaattgtaaa
     1981 cttatgcaaa acgaaacggc acgatacgtt aattaattga gaaaattgta gtcgtaacgg
     2041 caagaaatgc tccacatttg agaaaataat gattaccatt cccatttata acaagagcgt
     2101 gacgatgatt acgcttagcg aagcattgtg aagcagcaaa aatattggtt catcaaaggg
     2161 agtcgtcatg catttacgtc atctgttttc atcgcgcctg cgtggttcat tactgttagg
     2221 ttcattgctt gttgcttcat cattcagtac gcaggccgca gaagaaatgc tgcgtaaagc
     2281 ggtaggtaaa ggtgcctacg aaatggctta tagccagcaa gaaaacgcgc tgtggctcgc
     2341 cacttcgcaa agccgcaaac tggataaagg tggcgtggtt tatcgtcttg atccggtcac
     2401 tctggaagtg acgcaggcga tccataacga tctcaagccg tttggtgcca ccatcaataa
     2461 cacgactcag acgttgtggt ttggtaacac cgtaaacagc gcggtcacgg cgatagatgc
     2521 caaaacgggc gaagtgaaag gccgtctggt gctggatgat cgtaagcgca cggaagaggt
     2581 gcgcccgctg caaccgcgtg agctggtagc tgatgatgcc acgaacaccg tttacatcag
     2641 tggtattggt aaagagagcg tgatttgggt cgttgatggc gagaatatca aactgaaaac
     2701 cgccatccag aacaccggta aaatgagtac cggtctggcg cttgatagca aaggcaaacg
     2761 tctttacacc actaacgctg acggcgaatt gattaccatc gacaccgccg acaataaaat
     2821 cctcagccgt aaaaagctgc tggatgacgg caaagagcac ttctttatca acatcagcct
     2881 tgataccgcc aggcagcgtg catttatcac cgattctaaa gcggcagaag tgttagtggt
     2941 cgatacccgt aatggcaata ttctggcgaa ggttgcggca ccggaatcac tggctgtgct
     3001 gtttaacccc gcgcgtaatg aagcctacgt aacgcatcgt caggcaggta aagtcagtgt
     3061 gattgacgcg aaaagctata aagtggtgaa aacgttcgat acgccgactc atccaaacag
     3121 cctggcgctg tctgccgatg gcaaaacgct gtatgtcagt gtgaaacaaa aatccactaa
     3181 acagcaggaa gctacccagc cagacgatgt gattcgtatt gcgctgtaat agatatggcg
     3241 agggtgaaaa cggtgagact tgcctgcctt ttatcacggc gtatccggca ctttcgtcag
     3301 taatctgaat ttgcccggca cgtagccggg caattttgca attaactcgc cgtttcaggc
     3361 ttaaacacaa tttcctgttt ttcttcatct tcctcgacga ctggcgcagt gctgtgaatt
     3421 tcagcaacgc gtttgcgcac accaaaccag cctataacca gcagaatacc gataatcggc
     3481 agcgccgcga tagtataagt cccgttcggg taatcgaacg ccatcagcac aaggacactc
     3541 agtaaaaaca gtaatgtcag ccaggaagtg aagggcgcgc caggcagttt aaaactgaca
     3601 tccgctgctt tgccttcttt aatcgcttta cgcaggcgca tctggcacac gatgataaac
     3661 gcccatgaag cgatgattcc cagcgacgcg aagttcaaca caatctcaaa tacgcgcgac
     3721 ggcaccagat agttgaggaa tacgccgacg acatacacaa ccagtgtcgc tagaatcccg
     3781 gcatacggca catgctgacg actcattttc gccataaaac tcggtgcgga accgcccatc
     3841 gccattgagc gcagaatacg tccggtgcag tacagacctg agttcaggct ggagagggca
     3901 gcggtcagca ccacaatgtt cataatgctg ccgatatatg gcacacccag tttagagaaa
     3961 aacgtcacga acggactttg ccccgcctga tacgcgctcc acggcaataa cataaccagc
     4021 aacaccacgg agccgacgta aaacaggcca atacgccaaa tcacactgtt aatggcttta
     4081 ggcaccatgg tctgcggatc tttacattct ccggcagctg tacccaccat ttcaatggag
     4141 gcaaaagcaa acactacgcc ctgaatcaac accagcgcag gcagcaaacc gtgggggaag
     4201 aagccgccat tatcggtgat taaatgaaag ccagtggtgt tgccatccag cggctgacca
     4261 ctaccgagga acactgtacc cacgaccaaa aaggtcacaa tggcgagcac tttaataagc
     4321 gcaaaccaga actccatctc cgcaaaccat ttcacaccga tcatattcat ggtgccaacg
     4381 atggtaagtg cagcgagcgc aaagacccac tgcggcacgc cgccaaacgc accccagtaa
     4441 tgcatataca gagcgacggc ggtaatatca acaatccccg tcatcgccca gttgatgaag
     4501 tacatccagc cagcaacata agcggctttc tcacccaaaa actcacgggc ataagagaca
     4561 aaactgccac tggaagggcg gtgtagcacc agctcaccca atgcacgcag aataaaaaac
     4621 gaaaacaagc cacaaattaa ataaaccagt gccagtgctg gacccgccat ttgcagtcgg
     4681 gctcctgcac ctaaaaacaa gccggtgcca atcgcgccgc caatggcgat catctgtacc
     4741 tggcgattgc ccatcgcttt gtgatacccc tcttcgtggg cattaagcca gcggcgtttc
     4801 gcggcgtgtt gatctgaagt gtcggtgtcg tgtttactca ttgctctccc tgattgcttt
     4861 aatgaaaaag tcatataagt tgccatgaac aatgtttatt catcctggcg atgatagtta
     4921 tcaccattat tctttatggg tataaaacta tcggcgcagc atcctacccg ttatcgatat
     4981 acgatgcaaa acatcccctt acaatcctga aggggattca tacaactgac gaaaaaatga
     5041 caaatacttt tgctggttaa cctgtgtact gtcctacact taatctttaa aagattgtga
     5101 gggacatatg attaaggtgt atggcgtacc cggatggggc tctacaatca gtgagctgat
     5161 gctaacactg gctgatattc cttatcagtt cgttgatgtc agtggttttg accacgaggg
     5221 aacttcacgc gagttattga aaaccctgaa cccgttgtgt cagattccga cactgacgct
     5281 ggaaaatgac gagattatga cggagacagc ggcgattgca ttgatggttc tcgatcgtcg
     5341 cccggacctt gcgccaccgg ttgggcgcgc cgaacgtcag cagtttcaac ggctattggt
     5401 ctggctggtt gctaatgtct atccaacatt cactttcgcc gattaccctg agcgttgggc
     5461 tcctgacgca cctgaacagt taaagaagaa tgttattgaa tatcgtaaat cgctttatat
     5521 ctggctgaat tcacaattga ccgctgaacc ttatgcgttt ggtgagcaac taacgctggt
     5581 ggactgttat ctttgcacta tgcgcacatg ggggcctggg catg
//
LOCUS       DAJTYD010000207         5617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTYD010000207 DAJTYD010000000
VERSION     DAJTYD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.209"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..189
                     /gene="raiA"
                     /locus_tag="OO585_003616"
     CDS             <1..189
                     /gene="raiA"
                     /locus_tag="OO585_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6631061.1"
                     /translation="ATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEAR
                     RAATSVKDANFVEEVEEE"
     misc_feature    292..417
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            293..340
                     /gene="pheL"
                     /locus_tag="OO585_003617"
     CDS             293..340
                     /gene="pheL"
                     /locus_tag="OO585_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6631062.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            440..1600
                     /gene="pheA"
                     /locus_tag="OO585_003618"
     CDS             440..1600
                     /gene="pheA"
                     /locus_tag="OO585_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6631063.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGLADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1643..2764)
                     /gene="tyrA"
                     /locus_tag="OO585_003619"
     CDS             complement(1643..2764)
                     /gene="tyrA"
                     /locus_tag="OO585_003619"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6631064.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2775..3845)
                     /gene="aroF"
                     /locus_tag="OO585_003620"
     CDS             complement(2775..3845)
                     /gene="aroF"
                     /locus_tag="OO585_003620"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6631065.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4053..4418
                     /locus_tag="OO585_003621"
     CDS             4053..4418
                     /locus_tag="OO585_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6631066.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4568..5086
                     /locus_tag="OO585_003622"
     CDS             4568..5086
                     /locus_tag="OO585_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6631067.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5076..>5617
                     /locus_tag="OO585_003623"
     CDS             5076..>5617
                     /locus_tag="OO585_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6631068.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRH"
BASE COUNT         1505 a         1444 c         1275 g         1393 t
ORIGIN      
        1 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
       61 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
      121 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
      181 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
      241 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
      301 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
      361 tgaaacagaa tgcgaagacg aacaataagg cctcccaaac cggggggcct tttttattga
      421 taacaaaaaa ggcaacacta tgacatcgga aaatccatta ctggcgctgc gagagaaaat
      481 cagcgcgctg gatgaaaaat tattagcatt actggcagag cggcgcgaac tggccgtcga
      541 ggtgggaaaa gccaaactgc tctcgcatcg cccggtacga gatattgatc gtgaacgcga
      601 tttactggaa agattaatta cgctcggtaa agcgcaccat ctggacgccc attacattac
      661 tcgcctgttc cagctcatca ttgaagattc cgtattaact cagcaggctt tgctccagca
      721 acatctcaat aaaattaatc cgcactcagc acgcatcgct tttctcggcc ccaaaggttc
      781 ttattcccat cttgcggcgc gccagtatgc tgcccgtcac tttgagcaat tcattgaaag
      841 tggctgcgcc aaatttgccg atatttttaa tcaggtggaa accggcctgg ccgactatgc
      901 cgtcgtaccg attgaaaata ccagctccgg tgccataaac gacgtttacg atctgctgca
      961 acataccagc ttgtcgattg ttggcgagat gacgttaact atcgaccatt gtttgttggt
     1021 ctccggcacg actgatttat ccaccattaa tacggtctac agccatccgc agccattcca
     1081 gcaatgcagc aaattcctta atcgttatcc tcactggaag attgaatata ccgaaagtac
     1141 gtctgcggca atggaaaagg ttgcacaggc aaaatcaccg catgttgctg cgttgggaag
     1201 cgaagctggc ggcactttgt acggtttgca ggtactggag cgtattgaag cgaatcagca
     1261 acaaaacttc acccgatttg tggtgttggc acgtaaagcc attaacgtgt ctgaccaggt
     1321 tccggcgaaa acgacgttgt taatggcgac cgggcaacaa gccggtgcgc tggttgaagc
     1381 gttgctggtg ctgcgcaacc acaatctgat tatgacccgt ctggaatcac gcccgattca
     1441 cggtaatcca tgggaagaga tgttttatct ggatattcag gccaatcttg aatcagcgga
     1501 aatgcaaaaa gcattgaaag agttaggtga aattacccgt tcaatgaagg tattgggctg
     1561 ttacccaagt gagaacgtag tgcctgttga tccaacctga tgaaaaggtg ccggatgatg
     1621 tgaatcatcc ggcactggat tattactggc ggttgtcatt cgcctgacgc aataacacgc
     1681 ggctttcact ctgaaaacgc tgtgcgtaat cgccgaacca gtgctccacc ttgcggaaac
     1741 tgtcaataaa cgcctgctta tcgccctgct tcagcaactc aatcgcctcg ccgaaacgct
     1801 tatagtaacg tttgattaac gccagattac gctctgacga cataataatg tcggcataaa
     1861 gctgcggatc ctgagcaaac agtcgcccga ccatcgccag ctcaaggcgg taaatcggcg
     1921 aagagagcgc cagaagttgc tcaagctgaa cattttcttc tgccagatgc agcccataag
     1981 caaaagtagc aaagtggcgc agagcctgaa taaacgccat attctgatcg tgctcgacag
     2041 cgctaatacg atgcagccga gcgccccaga cctgaatttg ctccagaaac cattggtatg
     2101 cttccggctt acgtccatca caccagacca caacttgctt tgccaggcta ccgctgtccg
     2161 ggccgaacat cgggtgtaac cccagtaccg ggccatcgtg cgccgccagc atggcctgta
     2221 atggtccatt tttcactgat gccagatcaa ccagaataca atctttcggt aaaggcggta
     2281 atttgccgat aacttgctca gtaacgtgga ttggcacact aacaatcacc attccggcat
     2341 cggcaacaat atcagccgct cgatcccagt catgttgctc cagaatccgc acctgataac
     2401 ccgagagtgt cagcatcttc tcgaacaggc gtcccatctg accgccaccg ccgacgataa
     2461 ccaccggacg cagtgacgga caaagtgttt taaatccttt gtcgttttca ctggagtaag
     2521 attcacgcat cacccgacgc aaaacatcct caatcagatc tggcggtacc cccagagctt
     2581 ccgcctctgc acgacgcgag gccaacatag atgcctcgcg ctccggaaca taaataggca
     2641 gtccaaagcg gcttttcacc tcgcccactt cagcaaccag ttccagacgc ttcgctaata
     2701 aattcagcag cgctttatcg acttcatcaa tttgatcgcg taatgcggtc aattcagcaa
     2761 ccataataaa cctcttaagc cacgcgagcc gtcagctgcc cgttcagatc ctgatgaatt
     2821 tcacgcagca aggcatcggt catttcccag ctaatgcagg catcggttac ggatacaccg
     2881 tatttcattt cactgcgcgg ttgctcagaa gactgattgc cctcgtggat attactttcg
     2941 atcatcagac caataattga gcgattgcca tctttgattt gagcaaccac ggattctgcc
     3001 accgcaggct gacggcgata atctttattg gaattaccgt ggctgcaatc taccatcaga
     3061 gacgggcgca gtcccgcctg ttccatctct ttttcacatt gcgcaacatc cgcagggcta
     3121 tagttcggcg ctttaccacc gcgcaggatg acatgcccgt ccggattccc ctgagtttgt
     3181 agcaacgcaa cctgccctgc ctggttaatg ccaacaaaac ggtgcggctg ggcggcggcg
     3241 cgcatagcgt taattgctgt tgccagactg ccgtcggtgc cgtttttaaa accaaccggc
     3301 atggaaagcc cggaggccat ttcacggtga gtttgcgatt ccgttgtacg agcaccaatt
     3361 gctgaccagc taaacagatc gcccaggtat tgcgggctat tcggatctaa cgcttccgtc
     3421 gccagtggca gtcccatatt caccagctca agcagcaatt tacgcgcgat ctgcagcccg
     3481 gcttctacat caaaagagcc atccatatgg ggatcgttaa ttaacccttt ccagccgaca
     3541 gtggtacggg gtttttcaaa atagacgcgc attaccagat agaggctatc gctgacctct
     3601 gcggcaaggg ctttaaatcg acgagcatat tccagagcag tttccagatc atgaatggaa
     3661 caaggaccac aaactaccag cagacgagga tcgcgcccgg cgataatatc tgaaatggtt
     3721 ttacgcgagt cagcaatctg ggcttcttgt tgcaggctca atggaaaagc ggccttcagt
     3781 tgttccggag tcattaaaac ctgttcgtcg gtaatatgta cgttattcag cgcgtctttt
     3841 tgcatgatgg cgatcctgtt tatgctcgtt tgcgatagtt gatcctcagc gaggatgacg
     3901 taacgataac acataaagta aagttttcaa tccatatttc gtacgctttt ctttacacga
     3961 acaatttact cgcgctttga tgcttatttc gtacaaataa atttacaccc cctttaaaaa
     4021 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc gcccccttat
     4081 tggctttact ggttagcgga tgtcagattg atccttatac tcacgcgcca accttgacca
     4141 gcaccgactg gtatgatgtc ggtatggaag atgcgatatc gggcagcgcc ataaaagatg
     4201 acgatgcatt tggcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
     4261 gacaaaagaa aacttgccag accgatttta cttatgcccg gggactttcc ggtaaaagct
     4321 ttcctgccag ctgtaacaat gttgaaagtg ccagccaact gcatgaagta tggcaaaaag
     4381 gggctgatga aaatgccagc acaatacgat taaattaatt aaaaccccac aaataaatta
     4441 aacataagat tttactcatt gtgttttatt tctcacattg atgacgatcg catgaaaaaa
     4501 tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag aaggtgggga
     4561 tccctgaatg cgtttttctc accgactgtt cttacttctg attctcctgc tgacgggagc
     4621 accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg
     4681 tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat gtatcttttc
     4741 atcttcacgt tttagtaccg ccctacaaga gaatgccgcg acatctttgc cttatctgcc
     4801 tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt tttattttgg
     4861 caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa aggcgttatt
     4921 attaattgcc aaacaaaata ccgagtgcat tattggtagc gccttttgtc tgatcatcca
     4981 caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt
     5041 caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat aattctctta
     5101 ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc atatttatca
     5161 ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgaccctg aaacagtacg
     5221 cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa gcagctgtcg
     5281 tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag caagggcaat
     5341 tttcaactgc agaagtacgt gataagcagc aaaatattct ggcgtcctgg cattacaccc
     5401 gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc cccgccccca
     5461 tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta accgctcgcg
     5521 acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt tgtattctgc
     5581 tggcatcagg catcgcaatt accctcaccc gccattt
//
LOCUS       DAJTYD010000208         5441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTYD010000208 DAJTYD010000000
VERSION     DAJTYD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.211"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..915
                     /gene="adeQ"
                     /locus_tag="OO585_003624"
                     /gene_synonym="yicO"
     CDS             <1..915
                     /gene="adeQ"
                     /locus_tag="OO585_003624"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6631069.1"
                     /translation="IPLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSH
                     GVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIG
                     EVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDS
                     VSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYA
                     TAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMK
                     VCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            968..1420
                     /locus_tag="OO585_003625"
     CDS             968..1420
                     /locus_tag="OO585_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6631070.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1631..2821
                     /gene="nepI"
                     /locus_tag="OO585_003626"
     CDS             1631..2821
                     /gene="nepI"
                     /locus_tag="OO585_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6631071.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2862..3155)
                     /locus_tag="OO585_003627"
     CDS             complement(2862..3155)
                     /locus_tag="OO585_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631072.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3377..4195
                     /gene="nlpA"
                     /locus_tag="OO585_003628"
     CDS             3377..4195
                     /gene="nlpA"
                     /locus_tag="OO585_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR6631073.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4222..5121)
                     /locus_tag="OO585_003629"
     CDS             complement(4222..5121)
                     /locus_tag="OO585_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6631074.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5232..>5441)
                     /locus_tag="OO585_003630"
     CDS             complement(5232..>5441)
                     /locus_tag="OO585_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631075.1"
                     /translation="IGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWS
                     YHALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1300 a         1238 c         1393 g         1510 t
ORIGIN      
        1 attcccttaa gtttacgtat tggtatcacc agcggaattg gattatttat cgccttaatg
       61 ggattaaaaa atactggcgt tattgtcgcc aataaagaca cgctggtgat gattggcgat
      121 ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca
      181 tcacgtcatt ttcatgccgc ggtgctggtt tctatagtgg tgacgtcttg ctgtggatta
      241 tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc
      301 attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat cattttctcc
      361 tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac tgataaagcg
      421 ggcttaatag atggtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc
      481 gtcagttcgg tggcgggtgc gtttatcggc acctcgtctg ttactgccta tattgaaagt
      541 acttctggtg tggcagtcgg tggccgcacg gggctgactg cggttgtggt tggcgttatg
      601 ttcctgttgg ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc
      661 ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt taactgggat
      721 gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg
      781 atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt gtgcaccggg
      841 cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt
      901 attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg
      961 agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg
     1021 ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agtgatcggc tgaatatcga
     1081 tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt
     1141 acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat
     1201 ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt
     1261 acaaaaagcc cgtcttgccg cgccgattac cgatgctcaa gtcaggctgg cgctgggttt
     1321 tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc gctataacgc
     1381 gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg tcttatcagc
     1441 ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc cggtaatctt
     1501 tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag ttgcaaaaac
     1561 gttaaatgca tcacacattt caatgttaaa gtggtcggct tttcccctga aacatgccac
     1621 gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca tcacccgacc
     1681 gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta tcgttgagtt
     1741 tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg aaggggttgc
     1801 cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt ttattaccca
     1861 gacaattcag gctactgacc gctgctacgt tgttattttg tttgccgttt tgctgacgct
     1921 ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg gtcgtgcctg
     1981 tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt
     2041 gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct
     2101 ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt
     2161 taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat cattgccttc
     2221 actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac aacgtccggg
     2281 tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg ctttcttcac
     2341 gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct taacgctggt
     2401 gctgttgagt tttggtatcg ccagctttat tggtacgtcg ctttcgtcgt tcattcttaa
     2461 acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga gtgcgttggt
     2521 actgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta tctgggggct
     2581 aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc tggccgatca
     2641 ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata cctgtggcgc
     2701 ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga tgttgtccgg
     2761 cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga agaaatcctg
     2821 atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc cgggcattct
     2881 accttaccca acgcttccca ataattgctc tgattattac gttccatcgc cacgatggcc
     2941 tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca cgcttcatct
     3001 gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt ggttttctct
     3061 tttgcggact gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg catagcgaaa
     3121 aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta caaatgtgta
     3181 tcgaatgaag cataagtgta gtttgctttc atcgctctca tcattctgta cgaaacgcgc
     3241 ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt tttattctta
     3301 tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag gtaatcatta
     3361 ataaaaggat aaaaaaatga aactgacaac acatcatcta cggacagggg ccgcattatt
     3421 gctggccgga attctgctgg caggttgcga ccagagtagc agcgatgcaa aacacattaa
     3481 agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa aggtggcaaa
     3541 agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc taccgaacga
     3601 tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag catcgaccct ttcttgaaca
     3661 agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg tcttccctat
     3721 ggcgggttat tccaaaaaaa tcacaaccgt ggcgcaaata aaagaaggcg cgacagtggc
     3781 gatccccaac gacccgacca accttggtcg cgcactttta ctgttgcaaa aagagaagct
     3841 aattactctg aaagagggaa aagggttatt acataccgcg ctggatataa ccgataatcc
     3901 tcgtcatttg cagattatgg aactcgaggg ggcgcagctt ccgcgcgtac tggatgatcc
     3961 caaagttgat gtagcgatta tcagcaccac ttacattcag cagacagggc tttctccggt
     4021 gcacgacagc gtatttattg aagataagaa ttcgccgtat gtgaatattt tggtggcacg
     4081 ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc aatcacccga
     4141 agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct ggtaattctc
     4201 actcatgccg cgccggacga tttatttttc tggccggcga cgggaatcca ttgaaatcaa
     4261 aatcaccgat gacagaatca gcagcgttcc cagccagtcc ggtaatgtga acgtgatccc
     4321 cagcaacagc aaagagagta gcgcgctact taatggttct gcacagctca aaatgctggc
     4381 ttttggaccg ccaattaatt gtgctccttt caggtacaga ctaaatgtca gggacgtacc
     4441 aatgaccacc aaataaaaaa acgccagaat caaactgccg ttaacgacaa agtttgttcc
     4501 ttgtctggca taaaaaggca acagaatcag accgccaatc agcatactcc agccgacgac
     4561 tggtaacgtg ccatagcggg caattagcgt tgagggatag gtggtataga atgcagcagc
     4621 aaatgccgag gcaatgcccc agaacaacgc ggcaggagag agcgataatg acgtcggatt
     4681 accgtgtgtc accaataaaa aagtcccgac cagcgatgtc aaaatagcgc agaaaaccag
     4741 aatgcccggg cgcgatttac gcaccagtga gaaccaggcg acgataatcg tcggtgagag
     4801 gaattgcagc accgttgccg tggctgcgtt cgatttttcg atggttagca aaaaagtgag
     4861 ctgtacagtt agcgcgccaa ccacggaaaa aatcagcagg ctaatggcat ctttatgatt
     4921 gttaataata gaaaagattt tatcgccatg aacaaatgac agcgtcagta gaatcaaacc
     4981 ggcgaatatc aaacgcgtca tagtcaaaaa ctgcgacgac atctggcttt gctccatgat
     5041 gtattgcgcg cagacccctg aacttcccca caacacggcg gcaatcagaa cgttcagcat
     5101 cccctttctg gtggaaccca tttttccctc tgcatactgt tttgtcgttt atgtatgcag
     5161 gcatgatagc aaaaatggcg aggatggctc tgtttgcgcc acgccgattt ttcttagccc
     5221 agactggtga gctaaatatc tttaataaac agcaggcaaa tcatcgcaat acccaacatc
     5281 cctatcgcca gccagaacaa cgcatggtag ctccagattt caaccataat tccgtcaaca
     5341 gagccggcga taatccagcc gacgcgtgaa gtatttgcat ataacgttgt cgcagagcct
     5401 attttttcag gcattaagtc ctgaaaataa agcatgccga t
//
LOCUS       DAJTYD010000209         5418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTYD010000209 DAJTYD010000000
VERSION     DAJTYD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.212"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            760..2673
                     /locus_tag="OO585_003631"
                     /pseudo
     CDS             760..2673
                     /locus_tag="OO585_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2801..3523)
                     /locus_tag="OO585_003632"
     CDS             complement(2801..3523)
                     /locus_tag="OO585_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6631076.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3670..>5418
                     /gene="gyrA"
                     /locus_tag="OO585_003633"
     CDS             3670..>5418
                     /gene="gyrA"
                     /locus_tag="OO585_003633"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6631077.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLV"
BASE COUNT         1296 a         1354 c         1559 g         1209 t
ORIGIN      
        1 gaagttaccc tgggccgcag caactccctg atgaatgtcg gcgatacgca ttgccaggac
       61 gatccgcaag actgctacgg tctgacgata gggagtattg ataagtacca gaatcaggca
      121 gagctaaacg ttggctccac ccaacaaacc tttgtgcact cattgacggg ctttcagaat
      181 ggcactttaa atatcgatgc tggtggcaat gttactgtta atcaaggtag ttttgctggc
      241 accatcgaag gtgctggtca gctcaccatt gcgcaaaacg gcagctacgt gctgtcgggg
      301 gcgcagtcga tggcgctaac cggcgatata gtcgttgatg atggtgcggt gctttcgctg
      361 gaaggcgacg cggcagatct cgccgctctc caggacgatc cgcagtcgat cgtgttaaac
      421 ggcggtgtgc tcgatctctc tgatttctcc acctggcaga gcggcacatc atacaatgat
      481 ggccttgaag tcagtggcag cagcggaacg gttatcggca gtcaggatgt ggtagatctt
      541 gcaggtggcg acaatttgca tatccgcggc gacgggaaag atggcgtcta cgtggtggtc
      601 gatgcgagcg acgggcaggt aagtctggca aacaataata gttatttggg cacaacacaa
      661 atcgcctccg gtacgctgat ggtgagcgac aactcgcagc ttggagatac ccactataac
      721 cgccaggtta tctttaccga taagcaacaa gaaagcgtga tggagattac tgccaatgtc
      781 gatactcgct ctacgacgac tgagcatggg cgtgatattg aaatgcgcgc cgacggtgaa
      841 gtggcagttg atgcgggggt agacacgcag tggggcgcac tgatggctga cagcagcggg
      901 cagcatcagg atgagggtag cacatttact aaaacggggg cgggtacgct ggagctgacc
      961 gccagcggta caacgcagtc ggcagtacgt gtcgaagaag gcaccctgaa aggcgatgtt
     1021 gcggatatct ttccttatgc ttcgtcactg tgggtcggtg atggggcgac gttcgttact
     1081 ggcgcggatc aggatattca gtctatcgat gctacttcca gcggcactat cgatatcagc
     1141 gatggtacgg ttttgcgtct gaccgggcag gatacttccg tcgcccttaa tgcctcattg
     1201 tttaacggcg atgggacgct ggtgaatgct accgatggcg tgacgttgac aggtgagctt
     1261 aataccaacc ttgaaactga cagcctgacc tatcttgccg acgtgacggt taatggtgat
     1321 ctgaccaata cgtccggtgc ggttagcctg caaaatggcg tcgctggcga tacgctaacg
     1381 gtaaacggtg attatactgg cggcggtacg ctactgttcg acagcgaatt aaacggcgat
     1441 gactcggtaa gcgatcaatt ggtgatgaac ggtaatactg ctggcaacac aactgtggtg
     1501 gttaactcca ttacagggat tggtgagccg acatcgacag gcattaaagt ggttgatttc
     1561 gcagctgatc ccacgcagtt tcaaaacaat gcacagttca gtctggctgg cagtggctac
     1621 gtcaacatgg gagcgtatga ctacacgcta gtggaagata acaacgactg gtatctgcga
     1681 tcgcaagaag taacgccgcc atcgccacct gatccagacc cggacccaac acccgatcct
     1741 gacccaacac ccgatccaat ccccgcttac cagccggtgc tgaatgccaa agttggcggt
     1801 tatctcaata acctgcgggc ggcaaatcag gcgtttatga tggagcgacg cgatcacgct
     1861 ggtggcgatg gtcagacgct gaatttacgt gttatcggcg gacgttatca ttacacggcg
     1921 gcagggcaac tggctcagca ggaagacact tctacggtgc agcttagcgg cggcttgttt
     1981 agcgggcgct ggggcacgga tggcgagtgg atgcttgggg cggttggtgg ctacagtgat
     2041 aatcagggcg acagccgctc gaatatgacc ggaactcgcg ccgataacca gaaccacggt
     2101 tatgccgttg ggctgacctc aagctggtat cagcacggta attagaagca gggggcctgg
     2161 ctggatagct ggctgcaata cgcgtggttt aacaatgatg tttccgaaca tgaagatggc
     2221 gtggatcgtt accattcgtc ggggattatc gcctcgctgg aagccgggta tcagtggtta
     2281 ccggggcgtg gtgtggtgat tgaaccgcag gcgcaggtga tttatcagga cgtgcagcag
     2341 gatgatttta ccgccgctaa ccatgcgcgc gtgtcacaat cgcaggggga tgatattcag
     2401 acgcggctgg gtttacacag cgaatggcgt accgcggttg gtgtcacacc aacattagat
     2461 ctgaattatt atcacgatcc ccatgcgacg gaaattgaag aagatggcag cactatcagt
     2521 gacgatgcgg tgaagcgacg gggtgaaata aaagtgggag tcacgggcaa tatcagtcag
     2581 cgagtgtccc tgcgcggcag cgtggcgtgg cagaaaggga gtgatgattt tgcccagacg
     2641 gcagggtttt tgtcgatgac ggtgaaatgg taatcactgt ctagtcggca cggttgccgg
     2701 atgcggcgta aacgccttat ccggcctacg gggagcattt gtaggcctga taagacgcgg
     2761 caagcgtcgc atcaggcatt ggctcggcca aacaacaacc tcacttattc tgcgtgtgca
     2821 gcatatagtt cacatccacg ccggggccga gtttaaaagt attagtgagc gggttgtaat
     2881 gtagcccagt tatatgtcgc tctttaaggc ttgtctgatc tacccagcca agcagttctg
     2941 ccggtttaat aaacttcttc acatcatgcg tgcctttggg caccatgcgc aaaatatatt
     3001 ccgcgccaac caccgccatc agccatgact tgccgttgcg gttaagcgtc gagaaaaaga
     3061 catcgccgcc tggtttcacc agttgcgcac aggctctgac cactgactgc ggatcgggga
     3121 cgtgctccag catctccatg caggtcacca catcatactg cccggcatgt tttgccgcgt
     3181 gctcttccac ggtttcctgc acataatcca cctgaatgcc gctttccagc gcgtgcagtt
     3241 ttgccacctg caatggctca aagcccatat ccagaccggt caccgtcgcg ccttcgcgcg
     3301 ccatactctc ggccagaatg ccgccgccac aaccgacatc gagcaccttt ttgccaaata
     3361 aaccgccagc acgctcggca atatagccca gacgcagcgg gttaatgcgg tgcaacggtt
     3421 tgaactcacc ttcaagatcc caccagcggg aggcgacggc ttcaaattta gcgatctctt
     3481 cgtggtctac gttatggttt tccggcgatt tttcggcatt cattggcact tctactccgt
     3541 aattggcaag acaaacgagt atatcaggca ttggatgtga ataaagcgta taggtttacc
     3601 tcaaactgcg cggctgtgtt ataatttgcg acctttgaat ccgggataca gtagagggat
     3661 agcggttaga tgagcgacct tgcgagagaa attacaccgg tcaacattga ggaagagctg
     3721 aagagctcct atctggatta tgcgatgtcg gtcattgttg gccgtgcgct gccagatgtc
     3781 cgagatggcc tgaagccggt acaccgtcgc gtactttacg ccatgaacgt actaggcaat
     3841 gactggaaca aagcctataa aaaatctgcc cgtgtcgttg gtgacgtaat cggtaaatac
     3901 catccccatg gtgacgcggc ggtttatgac acgatcgtcc gtatggcgca gccattctcg
     3961 ctgcgttaca tgctggtaga cggtcagggt aacttcggtt ccatcgacgg cgactctgcg
     4021 gcggcaatgc gttatacgga aatccgtctg gcgaaaattg cccatgaact gatggccgat
     4081 ctcgaaaaag agacggtcga tttcgttgat aactatgacg gcacggaaaa aattcccgac
     4141 gtcatgccaa ccaaaattcc taacctgctg gtgaacggtt cttccggtat cgccgtaggt
     4201 atggcaacca acatcccgcc gcacaacctg acggaagtca tcaacggttg tctggcgtat
     4261 atcgatgatg aagacatcag cattgaaggg ctgatggaac acatcccggg gccggacttc
     4321 ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag aagcttaccg taccggtcgc
     4381 ggcaaggtgt atatccgcgc tcgcgcagaa gtggaagttg acgccaaaac cggtcgtgaa
     4441 accattatcg tccacgaaat tccgtatcag gtaaacaaag cgcgcctgat cgagaagatt
     4501 gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg cgctgcgtga cgagtctgac
     4561 aaagacggta tgcgcatcgt gattgaagtg aaacgcgatg cggtcggtga agttgtgctc
     4621 aacaacctct actcccagac ccagttgcag gtttctttcg gtatcaacat ggtggcattg
     4681 caccatggtc agccgaagat catgaacctg aaagacatca tcgcggcgtt tgttcgtcac
     4741 cgccgtgaag tggtgacccg tcgtactatt ttcgaactgc gtaaagctcg cgatcgtgct
     4801 catatccttg aagcattagc cgtggcgctg gcgaacatcg acccgatcat cgaactgatc
     4861 cgtcatgcgc cgacgcctgc agaagcgaaa actgcgctgg ttgctaatcc gtggcagctg
     4921 ggcaacgttg ccgcgatgct cgaacgtgct ggcgacgatg ctgcgcgtcc ggaatggctg
     4981 gagccagagt tcggcgtgcg tgatggtctg tactacctga ccgaacagca agctcaggcg
     5041 attctggatc tgcgtttgca gaaactgacc ggccttgagc acgaaaaact gctcgacgaa
     5101 tacaaagagc tgctggatca gatcgcggaa ctgttgcgta ttcttggtag cgccgatcgt
     5161 ttgatggaag tgatccgtga agagctggag ctggttcgtg aacagttcgg tgacaaacgt
     5221 cgtactgaaa tcaccgccaa cagcgcagac atcaacctgg aagatctgat cactcaggaa
     5281 gatgtggtag tgacgctctc tcaccagggc tacgttaagt atcagccgct ttctgaatac
     5341 gaagcgcagc gtcgtggcgg gaaaggtaaa tctgccgcac gtattaaaga agaagacttt
     5401 atcgaccgac tgctggtg
//
LOCUS       DAJTYD010000210         5341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTYD010000210 DAJTYD010000000
VERSION     DAJTYD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.213"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(892..1833)
                     /gene="fabY"
                     /locus_tag="OO585_003634"
                     /gene_synonym="yiiD"
     CDS             complement(892..1833)
                     /gene="fabY"
                     /locus_tag="OO585_003634"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6631078.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1878..2315)
                     /gene="dtd"
                     /locus_tag="OO585_003635"
     CDS             complement(1878..2315)
                     /gene="dtd"
                     /locus_tag="OO585_003635"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6631079.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2312..3185)
                     /locus_tag="OO585_003636"
                     /pseudo
     CDS             complement(2312..3185)
                     /locus_tag="OO585_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3179..3778)
                     /gene="yihX"
                     /locus_tag="OO585_003637"
     CDS             complement(3179..3778)
                     /gene="yihX"
                     /locus_tag="OO585_003637"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6631080.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3919..4806)
                     /locus_tag="OO585_003638"
     CDS             complement(3919..4806)
                     /locus_tag="OO585_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6631081.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4796..>5341)
                     /locus_tag="OO585_003639"
     CDS             complement(4796..>5341)
                     /locus_tag="OO585_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631082.1"
                     /translation="FGIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQ
                     KSLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFG
                     TKVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLI
                     EPTDEQETASYRYIREQSHEN"
BASE COUNT         1444 a         1346 c         1252 g         1299 t
ORIGIN      
        1 tctgataaat gaagaaaaac cgtattcatc gccattacca ccaccttacc tatattactc
       61 attaatgtat gtgcgaaaat tacgccggaa aatcattaaa aaaaagcgga tttttgcatg
      121 ttgttttgtt tttgcgaggc gttttccaga aataataaga gtgttgcagt tgtttcattt
      181 attactcaaa aatggtttag ttatcgccca ggatgaatta tgggtgctat gaatatatta
      241 tattcagaga tatttatttg cataatctga atggtgtagc cgaaaaacgt cagcatccta
      301 acagcaccag gatttaaggt gaaattaatc tttcatcaca gaccggtcag tactgccacc
      361 aaccggtctt tacttgctca tcgttataat tttgttggta acagttcggg aaagttgttt
      421 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      481 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      541 agtaaatgaa tgatactgac aatgatgcca gcccattatc tttgcataat gtttgtgagc
      601 tatagatttt aaaaatattt atgaggaaag tatggcacca gaggaggttt tgactatcaa
      661 aaatcgctca aacctgaact aagatgatga gttaagcatt atctgcgata attgccactc
      721 agctctacaa ataatattta aatgctcctg aacagtgatg tagcaagaca cgtatggtgc
      781 caacgaagaa taaaaattta tagagttctt attcaatatt accggcatag acagggaatg
      841 atgtaaatga ccggaatgaa tgagagaggc agcaccgccc ctctccataa gctactcttc
      901 ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga tatacgtgcc
      961 ttcaaacact gcacccggcg tctcgtcgcc aaagatttca acttgcatct gcacccgtgc
     1021 ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaagat cggctaccgc
     1081 atgaggttta ccgctaatcg gcttgctgta gcggatatgc gcatcggcca gaataatcgt
     1141 tccgccgagg tggcgttcgc gcagcatcag ccagataagc ccccaaccgg tgagcgttgc
     1201 cagcgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattac cggtttccgg
     1261 catggtggtg ataaattttt gcccggtata ttgctgaatg cgcacgccca ttttttcact
     1321 aagcgggatg tgttcgtacc acgcctgttg cagctgcgcg caccagtcgc cgcgatgcaa
     1381 aatatcatcc agagtggcaa caggtttaat catcaaaaaa tggcgaatcg gcgtggtggt
     1441 tggtgtggtg atttctccct gattaacaaa ccccagcttg gcgaaaaact ccaccgcgtc
     1501 ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg actccagggt
     1561 catcgccatc aacgtcccca gccctttgtc ctgcacgtcg ggatgaacgg ccataaagcg
     1621 aatggacgct tcattgtcgg cattaatata cagtcggcct accgccacca gattcccctg
     1681 ctcgtcgacg accatctgat gatgcgccat cgcatcccac gcgtcgcgtt ccgaaccttt
     1741 tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac gctctaactc
     1801 ttcttctgtt tgtggaaccc gaaggtgata catagcgata ctctctcttg tttcccgtga
     1861 caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc attgaccagc
     1921 gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt tcatctcttg ctggcggcag
     1981 cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcgcc tttggagaaa
     2041 cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac caccagtaca
     2101 ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct aaagatgcgg
     2161 tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc cttttcgaca
     2221 cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc cacggtgacg
     2281 ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct tcttgttcag
     2341 ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg ccaagcaaga
     2401 cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc agcacaccgt
     2461 aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc tttcctgctt
     2521 cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc gcggttaggt acgcggatgg
     2581 taggaacaat gctgtacagc aaccagaagg agatccacga caacagcaac ggaaaaatac
     2641 gcagcacgtt gtcgatgaca gtattgagat cgctcgccca gcgcagggag agcaaatagg
     2701 aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc atccagtaca
     2761 cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg ccagatggta ttcaacgcgc
     2821 tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcc ccaacggcgg
     2881 tcatcttgtt ggaattggca acaaattgtt cgatataccg ctgaataaca tcgccagtag
     2941 caggcagaaa gttggcaaaa ataaagtgac gtaactgaat gctgacgtcg gaaaacatgg
     3001 gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag agcaacgacc
     3061 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     3121 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     3181 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     3241 taatgcccag ctgattggct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3301 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3361 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3421 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3481 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3541 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3601 cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3661 tatgaaaact cttcttaagc gtagccagtg gaacacgcgt taaatcgctc caggctccca
     3721 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3781 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3841 ggcaataagg gtaatcttgt gatgctgatt gcgaaaaagc agagtaattt cactggcttt
     3901 ttcgcaatag atgaatggtt aagattcagg tgtaaattgc caacgagttt cgatgaccag
     3961 cggcctatct ggtgtcacca attccggcgt tagatttatt gcgttaggtg cctgagtcag
     4021 cgggttaata caggtggcat cgggctgttt atcaaatacc accagactgt ttgcagaaga
     4081 gatcatggtc atcgcgagtt taccaggcca cagtaactta accttcaccc cgttataaaa
     4141 accaaaacag tcatcccagg gaccggcttg aggagagata cgatttccag ttggcagttc
     4201 atcactaccc gcttcttctt gccagtccgc atcaaaaagc acctgcaacg attctgtatt
     4261 ttgcggagtc agctttttcg caaaccacgg atgccagcca gcggaggccg ggaatgtatc
     4321 ggcatatgaa tggacttcca gttgcaaaac cagcgcatcg ttctccagca aaaaagtctg
     4381 aatcacttcc ccttgccagg gccacggtga agccaggggc atacgtaaag tcagggaatc
     4441 tgctgttttg tcgatgattt cccacgttga gtaacaagcc ataccatgca acgcatgtgg
     4501 tggtttattg gcgggtaaag aataacattg cccaccagca ttcagcgtcg cattccccag
     4561 tcgccctgcc cacggcacca ttgggaaaca gccatactga aaagctctgc gctgcggctg
     4621 ccattggcgt aatacttcag aaccaaatgc ctttaacgag gttatcctgg caccatcatg
     4681 cggataaatc tgcaggtgaa tattgccttt ttccagcgtc actaactctc ctacaggccc
     4741 aggccatggc gcgtcaaacg cctgttgtag cgccaggtaa tgctcgtttt ttcgttcagt
     4801 tttcatgaga ttgttccctg atgtagcggt aagaagcggt ttcttgttca tcggtcggct
     4861 caatcagcca gacgcaaaca aaggaaagaa ctgagacagc aaataaaaac agagcgacat
     4921 accacggttt tcctccccct agcgccagca aacttgaggc gataaggggg cttagtccgc
     4981 caagaatcgc ggaaaattga taagcaaaag aaaggccggt atatcgtact ttggtaccga
     5041 acatgcgggt gaaaagtgtt ggctgcacgg caaacatcat cgtcggacct aagttgtagc
     5101 cgaggatcat actgcaccag atgatgagcg tacttttgct atccagtaat aagaagaaag
     5161 gaaacgcgaa caggacacaa aatcctgctc ccgacaaata cagacttttt tgacctatac
     5221 gatctgataa ccatccaacc aatggacaac tgaaaatacc gatcgccgag gcaatcaaca
     5281 tacccgtcag gggaatatct cgcgacaata ctaattggct ggaaatatag acaataccaa
     5341 a
//
LOCUS       DAJTYD010000211         5322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTYD010000211 DAJTYD010000000
VERSION     DAJTYD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.214"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..1386
                     /locus_tag="OO585_003640"
     CDS             1..1386
                     /locus_tag="OO585_003640"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6631083.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTCYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFN
                     IGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLD
                     ITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQ
                     IDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKK
                     AVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWY
                     RDLIANNGENI"
     gene            complement(1443..1661)
                     /gene="marB"
                     /locus_tag="OO585_003641"
     CDS             complement(1443..1661)
                     /gene="marB"
                     /locus_tag="OO585_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6631084.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1693..2076)
                     /gene="marA"
                     /locus_tag="OO585_003642"
     CDS             complement(1693..2076)
                     /gene="marA"
                     /locus_tag="OO585_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6631085.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2096..2530)
                     /gene="marR"
                     /locus_tag="OO585_003643"
     CDS             complement(2096..2530)
                     /gene="marR"
                     /locus_tag="OO585_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6631086.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2742..3407
                     /gene="marC"
                     /locus_tag="OO585_003644"
     CDS             2742..3407
                     /gene="marC"
                     /locus_tag="OO585_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6631087.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3432..4622)
                     /gene="ydeA"
                     /locus_tag="OO585_003645"
     CDS             complement(3432..4622)
                     /gene="ydeA"
                     /locus_tag="OO585_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6631088.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4772..>5322)
                     /gene="yneK"
                     /locus_tag="OO585_003646"
     CDS             complement(4772..>5322)
                     /gene="yneK"
                     /locus_tag="OO585_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6631089.1"
                     /translation="YLRQFDLLVLNFISRGNFVILPRLWSNSEVHRWFVNKDPNLITA
                     ILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDFFLFFANML
                     LHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNR
                     PRSPVRPPIPATAKTPDLPERH"
BASE COUNT         1435 a         1192 c         1304 g         1391 t
ORIGIN      
        1 ttggaaggtg gctggaatga aggaggaaaa ggcatcagta tcgctgatgt gatgactgct
       61 ggcgctcacg gggtgccgcg tgaagtgaca gaaggcgtta tcgacgggct taattatccc
      121 aatcatgaag caattgattt ttatcatcgc tataaaacag atattcagtt atttgccgag
      181 atgggattca aatgctttcg aacttccatt gcctggacac gaatctttcc gcaaggtgac
      241 gaacaggagc cgaatgaaga gggtttacaa ttttatgatg atctgttcga tgaatgcctt
      301 aagcagggaa tggaacctgt ggtgacgctt tcacattttg agatgcctta tcatctggtg
      361 acaaaatatg gtggctggcg aaaccgtaaa ctgatcgact tttttatccg cttcgcatca
      421 acggtcttca cgtgctataa agaaaaagta aagtactgga tgacgtttaa cgaaatcaat
      481 aatcaggtga atttcagcga aagcctgtgt ccatttacta attccggtat cttgtattcg
      541 ccagaggaag atatcaatga gcgcgaacaa ataatgtacc aggcggtaca ttacgagtta
      601 gttgccagtg ccctggcggt acagactgga aaatcgatca atcctgaatt taacattggc
      661 tgtatgatcg ccatgtatcc catctatcct ctgacgtgtg tacccaacga tatgatgatg
      721 gccacgaaag caatgcatcg tcgttactgg tttactgatg ttcatgctcg tggatattat
      781 ccgcaacata tgctgaatta ctttgccagg aaaggattca acctcgatat cacaccagaa
      841 gataacgcga ttcttgccag tggttgtgtc gactttatcg gctttagcta ctacatgtct
      901 tttacgacac aattttcgcc cgataacccg caactggatt atgttgaacc acgagatttg
      961 gtcagcaacc cttatatcga tacatccgaa tggggatggc aaattgatcc ggcagggcta
     1021 cgttattcac tcaactggtt ctgggatcat ttccagttgc cgctgtttat tgtcgaaaat
     1081 ggatttggtg cggttgacca gagacaagct gacggcacgg tgaacgatca ctatcgcatt
     1141 gattactttg cttcccatat tcgggaaatg aaaaaagccg tagttgaaga tggtgttgac
     1201 ttaattggct acaccccgtg gggctgcatt gacctggttt ctgccggaac aggggaaatg
     1261 aaaaagcgct acggaatgat ttatgtcgat aaagacaacg aagggaaggg aacgctggaa
     1321 cggatacgta aagcgtcgtt ttactggtat cgggatctca tcgccaacaa tggcgaaaat
     1381 atttgagact tgaggaagat attacggaga tgtcaggctc tgtgatcaga gcctgacaat
     1441 aactacatag cgtgttgatt ataatagggc acgccgagcg catccgactt atcactgcca
     1501 gtacccatgt gatttaaatc aaacggtggt tgttcctgcg atgggggaac aaccacgaca
     1561 ttggcacaag aagtaacaac tggctgcgtg gtttgttccg caacgccctg cgcggaaaag
     1621 agaataagcg cagctgctat tgcggatgaa agtggtttca tgattgcctc agtgacgttg
     1681 tcacgttatc aactagctat tgtaatgatt taatggatgt aaaaagcgcg attcgccctg
     1741 catattggtc atccggtatt tatgtggcgg aacatcaaag taatttttga aggttcgggt
     1801 cagagtttgt tgcgactcga agccatatcg ttctgccaga tagagtatcg gctcgttact
     1861 ttccttcagc ttttgcgcga tttccgtcat cttacggctg cggatgtatt ggcctaatga
     1921 atgaccggtt tcttttttaa acatccgttg caggtgccat ttagagtaac ctgaacgctc
     1981 tgacactttc tccagtgaca gtggcgattc caggttgtcc tcgatccagt ccaaaatgct
     2041 atgaatggta atagcgtcag tattgcgtct ggacatcgtc atacctcttt tttgtttacg
     2101 gcaggacttt cttaagcaaa tgctcaagtg ttgccacttc gtccgccgtc aggttttttg
     2161 ttaattcttg atgcaggtcc tggccaacta attgatggca ttgttcgcat attgccgcgc
     2221 cgctggtggt aagttttacc agtacgccgc gcttatcatt cgggttcggc aacctttcaa
     2281 cccagccttt acagaccagg cgatccagca tacgggtcag tgctccaagg tcgacagaca
     2341 acactttttt cagttcaacc ggagtaatac acgccgcgca gcggatagag cagagcacct
     2401 taaactgtgc cgcggtaata tccagcggag acagatactc gttaagcaga cgatctttct
     2461 tctgattaac catatggatt aagcgaccca atggaataat ttcattgaac agatcgctgg
     2521 tacttttcac attagttgcc ctggcaagta attagttgca gggggtaata ttgcccaggc
     2581 aagtataagt caaataaatg aattgaccga tgccacgttt tgctaaatcg gttcaagttt
     2641 tggtcaatgc gatgcaaggt tagctaaaga gtgacacttt atccttgttt accccttata
     2701 cttttcgctg ataatccaga tacactggat aacaaccacc aatgttagat ttgtttaaag
     2761 caattggcct ggggctggtg gtgttactgc cgttagctaa cccattaaca actgtcgcgt
     2821 tatttcttgg cctggcaggc aacatgaaca gtgccgaacg taatcgtcag tcgttgatgg
     2881 cctcggtgta cgtatttgcc atcatgatgg tggcgtatta cgccgggcaa ctggtgatgg
     2941 atacatttgg catttcgatt cccggtctgc gaattgcagg cggcttaatc gtcgcgttta
     3001 tcggttttcg gatgctcttt ccgcaacaga aagcgattga ttcaccggag gcgaaaagca
     3061 agtcggaaga gctggaagat gaacccagtg ccaatattgc ctttgtgccg ctagcaatgc
     3121 caagtaccgc tggtccggga accatagcga tgattattag ctcggcgtcc acagtgcgtc
     3181 agagttcaac cttcgctgat tgggtactga tggttgcgcc gccgctgatc tttttcctgg
     3241 tggcggttat tttgtgggga agcctacgca gctccggcgc aattatgcgg ctggtgggca
     3301 agggtggcat tgaagcgatc tcccgtctga tgggattcct gctggtatgt atgggtgtgc
     3361 agtttattat taacggcatc ctggaaatca ttaaaacgta ccattaaaaa aggcccgaat
     3421 gggcctttca actattgcgt ctgttcttcg agtgtcactg gccagcggcg aaatataatg
     3481 attgaccata ttaacgcggc aaaagcaggc accgcgccca cataaccaat catcgacatt
     3541 gaccagtgca aactcacctg attacctacc aacgcacccg ccccgattcc aatattaaat
     3601 atgccggaga atagcgccat cgcgacgtcg gtagcatctg gtgccagcgc cagcacttta
     3661 acctgcatac caagcccgat gatcatcatc gcgatccccc agaaaatact cagcaccccg
     3721 aggtgtattt cactgttcgc cgcaggtagc agcagtgcca gacacaccag caacagcgca
     3781 atcgccgtac tcaccaacgc agacgcatac tgattaccca gtttaccgaa aatcacgctg
     3841 ccaataatgc ccgcaccacc gagtaataac agtaatgccg tggcaaagtt ggcgctgaat
     3901 cccgcaatgt tttgcacaaa aggctcgata tagctgtatg ccgtgtaatg ggcggtgaca
     3961 accaccacag ttaacaaata aatgctcatc aatgccgggc ggcggaacaa tagcgggagg
     4021 cttttcaatg aaccggaatg ctcactgggc agtaagggaa gtaacttaat caggcacaaa
     4081 agggtgataa gcgccccaat accaatcgcg aagaaggtca ttcgccaacc gaaatactgc
     4141 cccacaatgc gcccgagagg taaacctaag accatcgcca gtgctgtacc ggtggcaatt
     4201 aaactcaagg cctgtgcccg cttcccggcc ggagccatac ggatcgccag agacgccgta
     4261 atcgaccaga aaatcgcatg tgcaaaagcc acaccaatgc gactgatcac cagaacggta
     4321 aagctccacg ataaaaacga cagtacgtgg ctggcaataa acaccacaaa caggcagatc
     4381 agtaatttgc gccgttcaac ctgactggtc attaacataa aaggcaatga cattagcgct
     4441 actacccatg cgtaaatggt caacatgatg ccgacctgag cggtttgcat gtgaaaactt
     4501 tgcgcaatgt cagagagcag gccaacaggg acaaattcgg tggtgttgaa gatgaaggcg
     4561 gcgactgcca gcgtaacgac ccgtagccac gccactttgc gggaaacagt gtttgttgtc
     4621 ataacagatt atcgggtgag ttgcgaaaaa gtgatgaaat gatcttaaaa cggttaatga
     4681 tgtgaataca atgcatttgt gatctggatc gcgttttccg tttctctgac gctgattctg
     4741 ttccgcgtca gagagtcggt aacatcacgg tttaatgacg ttcagggagg tctggcgttt
     4801 tggcggttgc gggtataggt ggtctgacag gggagcgagg cctgtttttc accaggtctt
     4861 cgtgagtaac ttttggcggc gtttcatttt ttacaaacag tttgagggtc ctgcgcgcca
     4921 tatgcttgat aattttatca tcacttaatg atgctggcat caacacgtca ttcagatcac
     4981 tttcattggg attcataaat tggtgatagt gcaataacat attggcgaaa aataaaaaaa
     5041 agtcctgaaa atgaggagat tcttgttctg ctaaaaggga taaaaagcag atgcagactt
     5101 cgggtagtac atgtttgtta gaacctaatg aatccatcag gctttgcaat aaatcctctt
     5161 ttaattcgcc gtctgttatg tcgagaatgg cggtgatcaa gttaggatct ttattgacga
     5221 accatctgtg aacctcagag ttactccata atcgggggag tatgacaaaa ttaccgcgag
     5281 agataaaatt caggacaagc aaatcaaact ggcgtaagta at
//
LOCUS       DAJTYD010000212         5189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTYD010000212 DAJTYD010000000
VERSION     DAJTYD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.215"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            484..1179
                     /gene="minC"
                     /locus_tag="OO585_003647"
     CDS             484..1179
                     /gene="minC"
                     /locus_tag="OO585_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6631090.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1203..2015
                     /gene="minD"
                     /locus_tag="OO585_003648"
     CDS             1203..2015
                     /gene="minD"
                     /locus_tag="OO585_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6631091.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2019..2285
                     /gene="minE"
                     /locus_tag="OO585_003649"
     CDS             2019..2285
                     /gene="minE"
                     /locus_tag="OO585_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6631092.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2764..2883)
                     /locus_tag="OO585_003650"
     CDS             complement(2764..2883)
                     /locus_tag="OO585_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6631093.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2844..3029)
                     /locus_tag="OO585_003651"
     CDS             complement(2844..3029)
                     /locus_tag="OO585_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631094.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3130..3303
                     /locus_tag="OO585_003652"
     CDS             3130..3303
                     /locus_tag="OO585_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631095.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3305..3649
                     /locus_tag="OO585_003653"
     CDS             3305..3649
                     /locus_tag="OO585_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6631096.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3653..3988
                     /locus_tag="OO585_003654"
     CDS             3653..3988
                     /locus_tag="OO585_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6631097.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4045..>5189)
                     /locus_tag="OO585_003655"
     CDS             complement(4045..>5189)
                     /locus_tag="OO585_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6631098.1"
                     /translation="EAFRQGDARIAAGAFEYQLKQQGKNWYLTSYQSVNEEDNSSEGN
                     SEPTETPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLR
                     QIGGHNAWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHS
                     SVSDYRSKGSVRGYCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYS
                     AKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIEND
                     ANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGA
                     SNLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
BASE COUNT         1409 a         1195 c         1231 g         1354 t
ORIGIN      
        1 tcatcatcaa tccgtaatca aaaccgctga cctggacgag ttacgatgac ctgacagaag
       61 tatcagacgt cttctgcggt ttctatgatt atgatagagc aattctatgg cggatattaa
      121 gataacgggt atctgttctt taatgatgct gcaaaaaatg gaaagttaag aaagcctggc
      181 gtggtgaaaa aatacacttg cctcaatact ggatatttaa acaggtctgg agatgctgtc
      241 acgattaggg tgcggaaaaa ggaagcgtaa ataagaacaa tcaaaagtac gacggcaatg
      301 ggttgattga caagggtatt ttttaagcta tgaatcagcg ccatttatca cagaatagac
      361 ttttactctg aataaatggg agggtgactt gcctcaatat aatccagact ataacatgcc
      421 ttatagtctt cggaacatca tcgcgcgctg gcgatgatta atagctaatt gagtaaggcc
      481 aggatgtcaa acacgccaat cgagcttaaa ggcagtagct tcactttatc tgtggttcat
      541 ctgcatgagg cagaacctaa ggttatccat caggcgctgg aagacaaaat cgctcaggcc
      601 cccgcatttt taaaacatgc ccccgttgta ctcaacgtca gtgcactgga agacccggta
      661 aactggtcag cgatgcataa ggcggtgtcg gcaaccggtt tgcgggttat tggcgtaagt
      721 ggctgcaaag atgcgcaact taaagccgaa attgaaaaga tggggctgcc tatcctgacg
      781 gaaggaaagg aaaaagcgcc gcgtccagct cccgcaccac aggctccagc gcaaaataca
      841 acgccggtca caaaaacgcg tttaatagat accccggtgc gttccggtca gcgtatttat
      901 gctccacaat gtgatctgat tgttacaagc cacgttagcg ctggggccga attgattgcc
      961 gatgggaaca ttcatgtcta tggcatgatg cgcggtcgtg cgcttgcagg ggcaagtggt
     1021 gaccgggaaa cgcaaatatt ttgtacgaac ctgatggcgg aactggtgtc catcgcaggt
     1081 gaatactggc tgagtgatca aatcccagca gaattttatg gcaaagcggc gcgactgcag
     1141 ttagtcgaaa acgctttgac cgttcaaccg ttaaattgat ccctttttaa caaggaattt
     1201 ctatggcacg cattattgtt gttacttcgg gcaaaggggg tgttggtaag acaacctcca
     1261 gcgcggccat cgccactggt ttggcccaga agggaaagaa aactgtcgtg atagattttg
     1321 atatcggcct gcgtaatctc gacctgatta tgggttgtga acgccgggtc gtttacgatt
     1381 tcgtcaacgt cattcagggc gatgcaacgc taaatcaggc gttaattaaa gataagcgta
     1441 ctgaaaatct ctatattctt ccggcatcgc aaacacgcga taaagatgcc ctcacccgtg
     1501 aaggggtcgc caaagttctt gatgatctga aagcgatgga ttttgaattt atcgtttgtg
     1561 actccccggc agggattgaa accggtgcgt taatggcact ctattttgca gacgaagcca
     1621 ttattaccac caacccggaa gtctcctcag tacgcgattc tgaccgtatt ttaggcattc
     1681 tggcgtcgaa atcacgccgc gcagaaaatg gcgaagagcc tattaaagag cacctgctgt
     1741 taacgcgcta taacccaggc cgcgtaagca gaggtgacat gctgagcatg gaagatgtgc
     1801 tggagatcct gcgcatcaaa ctcgtcggcg tgatcccaga ggatcaatca gtgttgcgcg
     1861 cctctaacca gggtgaaccg gtcattctcg acattaacgc cgatgcgggt aaagcctacg
     1921 cagataccgt agaacgtctg ttgggagaag aacgtccttt ccgcttcatt gaagaagaga
     1981 agaaaggctt cctcaaacgc ttgttcggag gataagttat ggcattactc gatttctttc
     2041 tctcgcggaa gaaaaacaca gccaacattg caaaagaacg gctgcagatt attgttgctg
     2101 aacgccgtcg cagcgatgca gaaccgcatt atctgccgca gttgcgtaaa gatattcttg
     2161 aggtcatttg taaatacgta caaattgatc ctgagatggt aaccgtacag cttgagcaaa
     2221 aagatggcga tatttctatt cttgagctga acgtgacctt accggaagca gaagagctga
     2281 aataagcccg ctgtaaaaac gcatttatct tcaaggcaga gtcatttctg ccttgagttt
     2341 ttcatccctc tcatccacgt tgtggtaaag cggcgagtat tcttgctgat actcctcatt
     2401 gctatttcca cctccccctt tacaccttaa ggctgttccc acgcacagaa caaaatgttt
     2461 atgcctggtt gagtaaataa cctcattgtt agtgtggata cactggggga tgataacccc
     2521 gcaatttcat tattcattgt tctcccatag gatgaggttt cccgccgtaa ctggcgggct
     2581 ttttttgccc cagaattttc ccttttagcc ccctgtaaac gaaaactgcg ccgaagcgca
     2641 gtttaatcgt tgatggtcag aacgaccagt tcacgatata tactgcgggt atcgactgat
     2701 ttcgattcca tgccattgga taaatgatag tagcgtctaa gtgacctaag ctctgctgca
     2761 acatcagccc gcaggcacca gaccagggga ttcatcctga agagacagcg caggtgtatt
     2821 gtgttcacca ctcaccaaag acatcatgat gaaatgatga tatcccgcat aagaaagagg
     2881 cattttttaa acgcagtgcg ctgaagtgta gttggataaa aaatcactct tttcagataa
     2941 tactgtcgta cgcttatctt tcgacaatga ggccgtcggc aaaataaaat gactaatgta
     3001 atcgttgctg atgaatatct tctgctcaca taaaaatcac acaataatat tgataaatca
     3061 gattatctta cctttacaac attgcgttaa cagtcaatga cacttcttga ataaaaatat
     3121 tgccaatcaa tgagttattc ctcatttaag attattctca ttcacgtaaa aaacataatt
     3181 cctatcatca cagccacgct cattttgaat tatctgaata atagcgagcg ttctttagtt
     3241 aagcagatct taattgaaga cgaaattatt gtgcgtgcaa cttacttaat accggacatc
     3301 taagttgaag aaaaaaatac ttgccttcgg gcttatctca gcattgttct gctccacgcc
     3361 agcgatggcg gatatgaatc gcaccacgaa aggtgcattg ctaggcgcag gcgttggttt
     3421 acttactggc aatggtgtta atggcgtact gaaaggtgca gctgttggcg ctggtgttgg
     3481 tgcagtaaca gaaaaaggcc gcgacggtaa aaatgcgcgt aaaggtgcaa aagtaggtgc
     3541 cgccgttggt gctgtcactg gcgttctgac cggcaatggc ctcgaagggg cgattaaagg
     3601 tgccgtgatt ggcggtacgg gtggtgcaat tctgggtaaa atgaaataag gaatgatgat
     3661 gaaaaaattt gctttactgg cgggcctgtt tgttttcgca ccaatgacct gggcgcagga
     3721 ctacaatatt aaaaacggtt taccgtcaga aacctatatt acttgcgcag aagccaacga
     3781 aatggcaaaa actgacagcg cacaggttgc agaaattgtt gcggtaatgg gtaatgccag
     3841 cgttgccagc cgtgatttaa aaattgagca atctccggaa ctgagcgcca aagtggtaga
     3901 aaaactgaat caggtttgcg ctaaagaccc gcaaatgctg ttgatcacgg caattgatga
     3961 caccatgcgt gctatcggca aaaaataata tttcatatgt accgcgtcga tgaatacgac
     4021 gcggtttttt agctaacggc gggatcagaa cttatatttc aggcccacca tcactgcggt
     4081 gtcattgtag ccattatcac ccagctgcac gcccacattc ccccagacgc tggccgctgg
     4141 attcaggttg ccatttacgc caagtttaat ttcccccaaa ttactcgccc catcttgttc
     4201 gactttcaca ccgttcattg agacagcaaa atctttactg ttatggatcc agttcatttc
     4261 aacaaaaggt tcaaagtcgt caccgtgcgg accgctgttt ttctcctgag tacgaataaa
     4321 ggtgcggaaa ccaagtcggg tctggatgtt gttatttgcg tcattctcaa tacgtgagcc
     4381 attggcttcg gtgtgcgcat tatgatcgac gcccatccag atagcctgtg cctgtggctg
     4441 gaatatccac gtgtatttcg ccgcttccag accaaaggat ttattcaggg caaagccgta
     4501 acccgcttcc agcgagacgg ttgcgccttt cgcactatag gattcatagg ctaattcatc
     4561 ccctttcacc gagtttttaa accagctata ttgcgcccag gcgtcaatgt atgcgccttt
     4621 tttactgatg tcatcggcaa accaagtggc atacagccct gcgcaatagc ctctgacact
     4681 ccctttcgaa cgataatccg acacgctgga atgagttgag ttgtagtcgc gggcataacc
     4741 agccatcact cccaaacgcc aactgtcact gtcggtaaaa ccaccggtta acagatcggc
     4801 ccctaactgc gagacgtagc gatgcgaggt cgttctcaac tgtccgttgc tgtcacgcca
     4861 ggcattatgc ccgccaattt gacgtagcca aagtcggctt gaacgctcct gttcagttac
     4921 aggatcgatg tagcgcattt cacccgcacg gtcgttcaga cgcataacaa aaagagtgtt
     4981 agcggctgcc aggttggcga cgtaacttcc agcttcgggg cgcaggacgg gggtaggcgt
     5041 ttctgttggt tctgaatttc cctctgagct gttatcctcc tcattcactg actgataact
     5101 tgtcaaatac cagtttttgc cttgttgttt tagttgatat tcgaaagctc cagctgcaat
     5161 ccttgcatca ccttgtctga aggcttctg
//
LOCUS       DAJTYD010000213         5179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTYD010000213 DAJTYD010000000
VERSION     DAJTYD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.216"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1835
                     /gene="xdhA"
                     /locus_tag="OO585_003656"
     CDS             <1..1835
                     /gene="xdhA"
                     /locus_tag="OO585_003656"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6631099.1"
                     /translation="HNGGNLLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMES
                     VTSLAWMEDDSRITIVSSTQIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVL
                     EEPMAAFLTSKLGGIPVKVSLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVL
                     SNTGAYASHGHSIASAGGNKVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFA
                     VESMLDDAATALGIDPVEIRLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEK
                     RRAECQNQQGNLRRGVGVACFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQ
                     GADTVFSQMVAETVGVPVSDVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLL
                     KEKIIAHAAVMLHQSAMNLTLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSA
                     ESSIKTTTNPPAFGCTFVDLTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMG
                     MGIGWALFEEMIIDAKSGVVRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSL
                     GEPPIIPVAAAIRNAVKMATGVAINTLPLTPKRLYEEFHLAGLI"
     gene            1846..2724
                     /gene="xdhB"
                     /locus_tag="OO585_003657"
     CDS             1846..2724
                     /gene="xdhB"
                     /locus_tag="OO585_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6631100.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            2721..3200
                     /gene="xdhC"
                     /locus_tag="OO585_003658"
     CDS             2721..3200
                     /gene="xdhC"
                     /locus_tag="OO585_003658"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6631101.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(3240..5018)
                     /locus_tag="OO585_003659"
     CDS             complement(3240..5018)
                     /locus_tag="OO585_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6631102.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
BASE COUNT         1333 a         1325 c         1250 g         1271 t
ORIGIN      
        1 tccataacgg tggcaattta ctgaaacaaa gcacgatgtc gacgggtaat gtccaacaaa
       61 caatcgatgc cgccgactac caggtacagg ggcactatca gacccccgtt attcaacatt
      121 gtcacatgga aagcgtaaca tcgctggcgt ggatggagga tgactcgcga attaccatcg
      181 tttccagcac ccagatcccg cacattgttc gccgcgtggt tggtcaggcg ctggatattc
      241 cctggtcatg cgtacgagtc atcaaaccat ttgtcggtgg cggttttggt aataaacagg
      301 atgtactgga agagccaatg gcggcattcc tgaccagcaa gcttggcggc attccggtga
      361 aagtttccct tagccgtgaa gagtgtttcc tcgcaacccg tacccgccac gcttttacca
      421 ttgacgggca aatgggcgtg aaccgcgacg gaacattgaa aggttatagt ctggatgttc
      481 tgtctaacac cggcgcttat gcatctcacg ggcactccat cgcttctgcg ggggggaata
      541 aagtcgctta cctttatcct cgttgtgcct acgcttacag ttcaaagacc tgctatacca
      601 acctcccctc ggctggtgcg atgcgtggtt atggcgcgcc acaagtcgta tttgccgttg
      661 agtctatgct tgatgacgcc gcgacagcgt taggtattga tcctgttgaa attcgtttac
      721 gcaacgccgc ccgcgaagga gatgctaatc cgctcacggg caaacgtatt tacagcgcag
      781 ggttgccgga gtgtcttgaa aaaggccgga aaatctttga atgggaaaaa cgccgtgcag
      841 aatgccagaa ccagcaaggc aatttgcgcc gcggcgttgg cgtcgcctgt tttagctaca
      901 cctctaacac ctggcctgtc ggcgtagaaa tagcaggcgc gcgccttctg atgaatcagg
      961 atggaaccat caacgtgcaa agcggcgcga cggaaatcgg tcagggtgcc gacaccgtct
     1021 tctcgcaaat ggtggcagaa accgtggggg ttccggtcag cgacgttcgc gttatttcaa
     1081 ctcaagatac cgacgttacg ccgttcgatc ccggcacatt tgcctcacgc cagagctatg
     1141 ttgccgcgcc tgcgctgcgc agtgcggcac tattattaaa agagaaaatc atcgctcacg
     1201 ccgcagtcat gctacatcag tcagcgatga atctgaccct gataaaaggc catatcgtgc
     1261 tggttgaacg accggaagag ccgttaatgt cgttaaaaga tttggcgatg gacgctttct
     1321 accaccctga acgcggcggg cagctctctg ctgaaagctc catcaaaacc accactaacc
     1381 caccggcgtt tggctgtacc tttgttgatc tgacggtcga tattgcgctg tgcaaagtca
     1441 ccatcaaccg catcctcaac gttcatgatt cagggcatat tcttaatcca ctgctggcag
     1501 aaggtcaggt acacggcgga atgggaatgg gcattggctg ggcgctattt gaagagatga
     1561 tcatcgatgc taaaagcggc gtggtccgta accccaatct gctggattac aaaatgccga
     1621 ccatgccgga tctgccacaa ctggaaagcg cgttcgtcga aatcaatgag ccgcaatccg
     1681 catacggaca taagtcactg ggtgagccac caataattcc tgttgccgct gctattcgta
     1741 acgcggtgaa gatggctacc ggtgttgcaa tcaatacact gccgctgacg ccaaaacggt
     1801 tatatgaaga gttccatctg gcaggattga tttgaggata acatcatgtt tgattttgct
     1861 tcttaccatc gcgcagcaac ccttgccgat gccatcaacc tgctggctaa caacccgcag
     1921 gccaaactgc tcgccggtgg cactgacgta ctgattcagc tccaccatca caatgaccgt
     1981 tatcgccata ttgttgatat tcataatctg gcggagctgc ggggaattac gctggcggaa
     2041 gatggctcgc tacgtatcgg ctctgcaacg acatttaccc agctaataga agatcctata
     2101 actcaacgtc atctcccggc gttatgtgct gcggccatgt ccattgctgg actgcagatc
     2161 cgtaacgtcg ctacctacgg tggaaatatt tgcaacggtg ccaccagcgc agattctgcc
     2221 acgccaacgc taatttatga cgcgaaactg gagatccact ccccgcgcgg tgttcgtttc
     2281 gtcccgatta atggctttca caccgggccg ggcaaagtgt ctcttgagca tgacgaaatc
     2341 ctcgtcgcct ttcattttcc gccacagccg aaagaacacg cgggcagcgc gcattttaaa
     2401 tatgccatgc gcgacgcaat ggatatttca acgattggct gcgccgcaca ttgccgactg
     2461 gataacggca atttcagcga attacgcctg gcatttggtg ttgccgcgcc aacgccgatt
     2521 cgctgccaac atgccgaaca gactgcacaa aatgcgccat taaacctgca aacgctggaa
     2581 gctatcagcg aatctgtcct gcaagatgtc gccccgcgtt cttcatggcg ggccagtaaa
     2641 gagtttcgtc tgcatctcat ccagacgatg accaaaaaag tgattagcga agccgtcgcc
     2701 gcggcggggg gaaaattgca atgaatcaca gcgaaacaat taccatcgaa tgcaccatta
     2761 acgggatgcc ttttcagctt cacgccgcac caggcacgcc gctctcggaa ttactccgcg
     2821 aacaaggact gctaagtgtc aaacaagggt gctgcgtggg tgaatgtggt gcctgtacgg
     2881 tgttggtcga cggcacagca atagacagtt gcttatacct tgccgcctgg gctgaaggaa
     2941 aagagatccg cacgctggaa ggtgaagcga aaggcggaaa actttctcat gttcagcagg
     3001 cttatgcgaa atccggcgca gtgcagtgcg ggttttgtac gcctggcctg attatggcta
     3061 ccacggcaat gctggcgaaa ccacgcgaga agccattaac cattacggaa attcgtcgcg
     3121 gactggcggg aaatctttgt cgctgcacgg ggtatcagat gattgtaaat acagttctgg
     3181 attgcgagaa aacgaagtaa aaggatatcc ggcctgaatt caggccggat tcactgaggt
     3241 tatgtgttta acaactcata tttcttaatc ttgcgataga gcgtagcaat gccgatgccc
     3301 agttcatcag caacttgctt cttgctgtta tgacgtgaaa gcgcctcgcg gatcatttgc
     3361 ttttccatct cctccagcgc cgtgccgccc gcatcatcga gtgacaggtg cgcctcactg
     3421 acctctgtta catcactttg ctccgttgtg ccattattca gcagatttgg cggcaatagc
     3481 gtgctgtcga taacttcacc tgaaggaact acgttaacca gatattccat caaattgctt
     3541 aactcgcgca ggtttccggg ccaacgatgc ttacgcaata tttcgacgac atcgggagca
     3601 atgccaggat aaaccgatcc cagacgacgg gtatgcagat gtaaaaagta atgcaccaat
     3661 agttcaatat cttcctgacg ttcacgcagc ggtggcagag ttatcgggat aacattaagt
     3721 cggtagaaga gatcttcgcg gaatttacct tcggcaatga actgggccaa attctgatta
     3781 gttgcagaaa tgatgcgaat gtcgacttgt attgggctac tggcaccaat cggcagaatt
     3841 tcacgcgcct caatagcgcg cagtaattta gcctgcaaca ttaatggcat atcacctatt
     3901 tcatcgagaa acagcgtgcc cgtattcgcc gcctgaatca accctgtttt accgttggca
     3961 gaagcgccag taaatgcgcc tttaacataa ccgaacagtt cgctttccag aagctgctcc
     4021 ggaatcgcgg cacagttgat agcaataaag ggtttattcc gtcttccgct caacttatgg
     4081 attgctcggg cgacgacttc tttacccgtg ccgctttcac caaccaccat aacgctggat
     4141 gggctgggtg caatacggct aatgagtcgt tttaattgcc gcataacacg gcactcgcca
     4201 accaattgtt caatatgtgg ttcatcaggt gcatttgcta cagaaaaact ggtatgcgat
     4261 tggtgaaatg ccattaaaaa taattgtcgg ccctgaatgt tatgcaattg accaatgatt
     4321 aattcacttt tatcgtccca cgaaacaata tgctgcatat gtccatgagt aaaattactc
     4381 tcaaatgtta atggtctgaa acggataggt ttcccaataa tattattttg tacaacacca
     4441 agtgttttta aggcagtctg attaacaaac tgaacccgac tttcatcatc aacaactaat
     4501 acgccctgat ccatattatc gatcatggtc gcgaatattt tactgatgtt atctcctggc
     4561 ccctgatcct ccagaagttt cgaaacaaaa atggtggata tatggcgaac ataatcagaa
     4621 aattcgcgta aattatcact gatatgctct tgttgctcgt gggtaacggc aatcaaactt
     4681 atcaccccaa cacaacgatc ctgtaaaatg acaggcgtac ccagaaatgc tttttcacgg
     4741 caattttctt tactatcgca accttcgcaa aggggatcga agcgagactg tgtcacaact
     4801 ttttcagttt tcgtttccag gacgtggcgg agcaggcgtg agttgccgct caactggcga
     4861 ccaagaaact tcccatacgc gcccgttccg gcgacgcgac acaagttttc atcaacgatc
     4921 tcaacctcaa gctgcaaaac gctggcaagc attctggcaa aacgctgaat tgtcggttga
     4981 atttgcatca atactgactg cgtagtagca agctccatag ctttaccttc cagacttact
     5041 taaaagtcga tcattgaaga cgttgatggt tcacagatca tgatgatatt aactcaggcg
     5101 aaattggctt tgataaaaac ataagatttt tatcattttc taatgaaatt atggaagaga
     5161 tatcacattt ctatatcaa
//
LOCUS       DAJTYD010000214         5162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTYD010000214 DAJTYD010000000
VERSION     DAJTYD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.217"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..343)
                     /gene="bamD"
                     /locus_tag="OO585_003660"
     CDS             complement(<1..343)
                     /gene="bamD"
                     /locus_tag="OO585_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6631103.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGL"
     gene            478..1458
                     /gene="rluD"
                     /locus_tag="OO585_003661"
     CDS             478..1458
                     /gene="rluD"
                     /locus_tag="OO585_003661"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6631104.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1455..2186
                     /gene="pgeF"
                     /locus_tag="OO585_003662"
     CDS             1455..2186
                     /gene="pgeF"
                     /locus_tag="OO585_003662"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6631105.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2316..4889
                     /gene="clpB"
                     /locus_tag="OO585_003663"
     CDS             2316..4889
                     /gene="clpB"
                     /locus_tag="OO585_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6631106.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1303 a         1226 c         1406 g         1227 t
ORIGIN      
        1 tcaggccacg catgtacatg acataatcga tattcggatg ggtcgggtta aggcgaataa
       61 aacgatcgat ggcagcctgt gctaacggca aatcggcgtt tttatagtag gcgtagatga
      121 gatccagctg cacctgctgc gaatacggac caaacggata gcgattatct aatgcttcca
      181 gttgcgttat tgcctgtctc cagttaccgt cctgcagctt ttgttgtgca gtcgcgtaaa
      241 tttcatttgg tggattatca ggtacttctt cctttgaccc cgagcaaccc gccaaaaaca
      301 ggcttagtgt ggcggctgcc accagatatt tcatgcgcgt catgacgttt tgactttcct
      361 caaaatgtaa tacgggagat tctctgttcc tgctcccggt taagaccagc tacaatagca
      421 cactatatta aacggcaaag ccgtaaaacc ccaacgataa acgaagaagc agtatatatg
      481 gcacaacgag tacagctcac tgcaacggtg tccgaaaacc aactcggtca acgcttagat
      541 caggctttgg ccgaaatgtt cccggattat tcacgttcgc gaataaaaga atggatcctc
      601 gaccagcgcg tgctggtaaa cggcaaagtt tgtgataagc cgaaagaaaa agtattgggt
      661 ggcgagcagg ttgccatcaa cgctgagatt gaagaagaag cgcgttttga accgcaggat
      721 atcccgctgg atatcgtcta tgaagatgaa gatatcatcg tcattaataa accgcgcgac
      781 ctggtggtac atcctggcgc gggtaacctg gatggcacgg tactgaatgc gttgcttcat
      841 tattatccgc ccattgccga tgtaccgcgt gcgggcatcg tccatcgtct ggataaagac
      901 accactggcc tgatggttgt ggcaaaaacc gttccggctc agacgcgttt agtcgaatct
      961 ttgcaaaggc gtgaaattac tcgtgagtat gaagcggtgg caattggtca tatgaccgct
     1021 ggcggtacag tagatgaacc aatcagccgc cacccgacca aacgtactca catggcggtg
     1081 catcccatgg gcaaaccggc ggtcactcac tatcgcatca tggaacactt ccgtgtgcac
     1141 acgcgtctgc ggttgcgtct ggaaaccgga cgtacgcacc agatccgcgt gcatatggcg
     1201 catattaccc atccgctggt gggcgatccg gtttatggtg gccgtccgcg tccgccaaaa
     1261 ggtgcttcgg aagcgtttat ctccacgctg cgtaagtttg accgccaggc gcttcatgca
     1321 actatgctgc gtctttatca cccgatctcc ggcatcgaaa tggaatggca tgcgcctatt
     1381 ccagaagata tggtggagtt gattgaggtt atgcgcgccg atttcgaaga gcataaggat
     1441 gaagtggact ggttatgagt aagctgattg tcccgcagtg gccgctgcca aaaggtgttg
     1501 cggcctgtag ctctacccgt atcggcggcg tgagcttgcc accttacgac tctctcaacc
     1561 ttggcgctca ttgtggtgat aacccggagc acgtagagga gaatcgcaag caactttttg
     1621 ctgcgggaaa tttgccttca aaaccggtct ggcttgagca ggtacacggc aaagatgtgc
     1681 ttaagctcac tggcgaacct tatgcctcaa aacgggcgga tgcctcttat agcaatacgc
     1741 ccggcacggt ttgcgcagtg atgactgccg actgcctccc tgtgctgttt tgcaatcgag
     1801 cgggaacgga agtcgccgcc gttcatgctg gctggcgtgg actgtgcgca ggcgtgctgg
     1861 aagagacggt ttcctgtttt gctgataaac ctgaaaacat tctcgcctgg ttagggccgg
     1921 caattggtcc acgcgcgttc gaagtggggg cggaggttcg cgaggcgttt atggcagtag
     1981 acgctaaagc aagtgcagct ttcattcagc atggtgataa gtatctggcg gatatttatc
     2041 agcttgcccg gcagcgtctg gcgaacgtgg gtgttgagca aattttcggc ggcgaccgtt
     2101 gtacatatac ggaaaatgag actttcttct cttatcgtcg cgacaagacc accggtcgta
     2161 tggcaagttt catttggctg atataaccta aagaatcaag acgattcggt acgcgtgatt
     2221 ttcttttcac attaatctgg tcaataacct tgaataattg agggatgacc tcatttaatc
     2281 tccagtagca actttgatcc gttatgggag gagttatgcg tctggatcgt cttactaata
     2341 aattccagct tgctcttgcc gatgcccaat cacttgcact cgggcacgac aaccaattca
     2401 tcgaaccact tcatttaatg agcgccctgc tgaatcagga agggggttcg gttagtcctt
     2461 tattaacatc cgctggcata aatgctggcc agttgcgcac agatatcaat caggcattaa
     2521 atcgtttacc gcaggttgaa ggtactggtg gtgatgtcca gccatcacag gatctggtgc
     2581 gcgttcttaa tctttgcgac aagctggcgc aaaaacgagg tgataacttt atctcgtcag
     2641 aactgttcgt tctggcggca cttgagtctc gcggcacgct ggccgacatc ctgaaagcag
     2701 caggggcgac caccgccaac attactcaag cgattgaaca aatgcgtgga ggtgaaagcg
     2761 tgaacgatca aggtgctgaa gaccaacgtc aggctttgaa aaaatatacc atcgacctta
     2821 ccgaacgagc cgaacagggc aaactcgatc cggtgattgg tcgtgatgaa gaaattcgcc
     2881 gtaccattca ggtgctgcaa cgtcgtacta aaaataaccc ggtactgatt ggtgaacccg
     2941 gcgtcggtaa aactgccatc gttgaaggtc tggcgcagcg tattatcaac ggcgaagtgc
     3001 cggaaggatt gaaaggccgc cgcgtactgg cgctggatat gggcgcgctg gtggctgggg
     3061 cgaaatatcg cggtgagttt gaagaacgtt taaaaggcgt gcttaacgat cttgccaaac
     3121 aggaaggcaa cgtcatcctg tttatcgacg aattacatac catggtcggc gcgggtaaag
     3181 ccgatggcgc aatggacgcc ggaaacatgc tgaaaccggc gctggcgcgt ggtgaattgc
     3241 actgcgtagg tgccacgacg cttgacgaat atcgccagta catcgaaaaa gatgctgcgc
     3301 tggaacgtcg tttccagaaa gtgtttgttg ccgagccttc tgttgaagat accattgcga
     3361 ttctgcgtgg cctgaaagaa cgttacgaat tgcaccacca tgtgcaaatt actgacccgg
     3421 caattgttgc agcagcgacg ttgtctcatc gctacattgc tgaccgtcag ctgccggata
     3481 aagccatcga cctgatcgat gaagcagcat ccagcattcg tatgcagatt gactcaaaac
     3541 cagaagaact cgaccgactc gatcgtcgta tcatccagct caaactggaa caacaggcgt
     3601 taatgaaaga gtctgatgaa gccagtaaaa aacgcctgga tatgctcaac gaagaactga
     3661 gcgacaaaga acgtcagtac tccgagttag aagaagagtg gaaagcagaa aaggcatcgc
     3721 tttctggtac gcagaccatt aaagcggaac tggaacaggc gaaaatcgct attgaacagg
     3781 ctcgccgtgt gggggacctg gcgcggatgt ctgaactgca atacggcaaa atcccggaac
     3841 tggaaaagca actggaagcc gcaacgcagc tcgaaggcaa aactatgcgt ctgttgcgta
     3901 ataaagtgac cgacgccgaa attgctgaag tgctggcgcg ttggacgggg attccggttt
     3961 ctcgcatgat ggaaagcgag cgcgaaaaac tgctgcgtat ggagcaagaa ctgcaccatc
     4021 gcgtcattgg tcagaacgaa gcggttgatg cggtatctaa cgctattcgc cgtagccgtg
     4081 cggggttggc ggatccaaat cgcccgattg gttcattcct gttcctcggc ccaacgggtg
     4141 tggggaaaac tgagctttgt aaggcgctgg cgaactttat gtttgatagc gacgaggcga
     4201 tggtccgtat cgatatgtcc gagtttatgg agaaacactc ggtgtctcgt ctggttggtg
     4261 cgcctccggg atatgtcggt tatgaagaag gtggctacct gaccgaagct gtgcgtcgtc
     4321 gtccgtattc cgtcatcctg ctggatgaag tggaaaaagc gcatccggat gtcttcaaca
     4381 ttctgttgca ggtactggat gatggtcgtc tgactgacgg gcaggggaga acggtcgact
     4441 tccgtaatac ggttgtcatt atgacctcta acctcggttc cgatttgatt caggaacgct
     4501 tcggtgaact ggattatgcg cacatgaaag agctggtgct cggtgtggta agccataact
     4561 tccgtccgga attcattaac cgtattgatg aagtggtggt cttccatccg ctgggtgaac
     4621 agcacattgc ctcgattgcg cagattcagt tgaaacgtct gtacaaacgt ctggaagaac
     4681 gtggttatga aatccacatt tctgacgagg cgctgaaact gctgagcgag aacggttacg
     4741 atccagtcta tggtgcacgt cctttgaaac gtgctatcca gcagcagatc gaaaacccgc
     4801 tggcacagca aatactgtct ggtgaattgg ttccgggtaa agtgattcgc ctggaagtta
     4861 atgaagaccg gattgtcgct gtccagtaat gataaaacga gcccttcggg gctcgttttt
     4921 gtctataagt tagacggaaa agactatatt taagatgttt tgcctgaaaa atgagcgaac
     4981 gataaagttt ttatattttt cgcttgtcag gccggaataa ctccctataa tgcgccacca
     5041 ctgacacgga acaacggcaa acacgccgcc gggtcagcgg ggttctcctg agaatctcaa
     5101 cagagaaaag caaaaaaatg cttgactctg tagcgggaaa gcgcattatg tacaccccgc
     5161 gc
//
LOCUS       DAJTYD010000215         5098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTYD010000215 DAJTYD010000000
VERSION     DAJTYD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.218"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..139
                     /locus_tag="OO585_003664"
     CDS             <1..139
                     /locus_tag="OO585_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631107.1"
                     /translation="EEQVNWLRKRGVRYCQGWFLAKAMPPQVFMQWMEQLPARELTRG
                     Q"
     gene            complement(142..465)
                     /gene="soxS"
                     /locus_tag="OO585_003665"
     CDS             complement(142..465)
                     /gene="soxS"
                     /locus_tag="OO585_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6631108.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            551..1015
                     /gene="soxR"
                     /locus_tag="OO585_003666"
     CDS             551..1015
                     /gene="soxR"
                     /locus_tag="OO585_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6631109.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            1561..2910
                     /gene="ghxP"
                     /locus_tag="OO585_003667"
                     /gene_synonym="yjcD"
     CDS             1561..2910
                     /gene="ghxP"
                     /locus_tag="OO585_003667"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6631110.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3062..4711
                     /locus_tag="OO585_003668"
     CDS             3062..4711
                     /locus_tag="OO585_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6631111.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4865..>5098)
                     /locus_tag="OO585_003669"
     CDS             complement(4865..>5098)
                     /locus_tag="OO585_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6631112.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1053 a         1226 c         1440 g         1379 t
ORIGIN      
        1 tgaggagcag gttaactggc tgcgcaaacg cggcgtgcgc tattgccagg gatggtttct
       61 tgcgaaggcg atgccgccgc aggtgtttat gcaatggatg gagcaattac ccgcgcggga
      121 gttaacgcgc gggcaataaa actacaggcg gtggcgataa tcgctgggag tgcgatcaaa
      181 ctgccgacgg aaaacgcggg agaaggtctg ctgcgagaca taacccaggt ccattgcgat
      241 atcaaaaatc ggacgctcgg tggtgcgcaa ctcaacggcg gccagtaaca ggcgacgttg
      301 gcgaatgtaa tcgccaagcg tctgatgcgt caccgtgcgg aacattcgtt gcaagtacca
      361 ctttgaatag cctgattttt ttgcgactac atcaatgtta agcggctggt caatatgctc
      421 gtcaatccat gcgataagat cctgaataat tttctgatgg gacataaatc tgcctctttt
      481 cagtgttcag ttcgttaatt catctgttgg ggagtataat tcctcaagtt aacttgaggt
      541 aaagcgattt atggaaaaga aattaccccg cattaaagcg ctgctaaccc ccggcgaagt
      601 ggcgaaacgc agcggtgtgg cggtatcggc gctgcatttc tatgaaagta aagggttgat
      661 taccagtatc cgtaacagcg gcaatcagcg gcgatataga cgtgatgtgt tgcgatatgt
      721 tgcaattatc aaaattgctc agcgtattgg cattccgctg gcgaccattg gtgaagcgtt
      781 tggcgtgttg ccagaagggc atacgttaag tgcgaaagag tggaaacagc tttcgtccca
      841 atggcgagaa gagttggatc ggcgcattca taccttagtg gcgctgcgtg acgaactgga
      901 cggatgtatt ggttgtggct gcctttcgcg cagtgattgc ccgttgcgta acccgggcga
      961 ccgcttagga gaagaaggta ccggcgcacg cttgctggaa gatgaacaaa actaaagcgc
     1021 cacaagggcg ctttagtttg ttttccggtc tttgtctttc tctctatccc gctggtacac
     1081 aggagggttt cccccgacgt caacacacct cattcgagca cgtggtggag gttccggttg
     1141 gtgttgatgc tttaattgta tgtcaccgac gtttcttcgc cagtgtaaaa gtatactttt
     1201 taaccgcaat atttttgtca tctcagacga ttttttatcg caatcctgaa cggtatacgg
     1261 ctcgataacg ctgcaatctt gcgcaccgac gataacgttt gcgcatcaat tgcctgtttt
     1321 ttcatcgtca agacaataaa agagaaaaaa gcagcaaact tcggttgaaa aagccgctat
     1381 gatcgccgga taatcgtttg ctttttttac cacccgtttt gtatgcgcgg agctaaacgt
     1441 ttgctttttt gcgacgcagt aaattgtcgc aaacctggag caggaagata acgtttcgct
     1501 ggcaggggat tgtccgccac gcatcttgac gaaaattaaa ctctcagggg atgttttctt
     1561 atgtctacgc catcagcgcg taccggcggt tcactcgacg cctggtttaa aatttcacaa
     1621 cgtggaagca ctgtccgtca ggaagtggtt gccgggttaa caacgtttct ggcgatggtc
     1681 tactcggtca tcgtcgttcc aggtatgttg ggtaaagcag gcttcccgcc tgcggcagtt
     1741 ttcgttgcaa cctgtctggt tgccggactc ggttctatcg tgatgggtct gtgggctaat
     1801 ctgccgttgg cgattggttg cgccatctcc ctgacggcgt ttaccgcatt cagcctggtg
     1861 ctggggcaac atattagcgt acctgtcgcg ctgggtgccg tgttcctgat gggtgtgctg
     1921 tttacggtaa tttctgccac gggtatccgt agctggattt tgcgcaactt gccgcacggt
     1981 gtggcgcacg gcacggggat cggtatcggt ctgttcctgc tgctcattgc cgctaatggt
     2041 gttggtctgg tgattaaaaa cccgcttgat ggtctgcccg ttgcgctggg tgatttcgcg
     2101 accttcccgg tgattatgtc actggtaggt ctggcggtga tcatcggcct ggaaaaactg
     2161 aaagtccctg gtggcattct gctgaccatt atcggtatct caattgtcgg tttgatcttc
     2221 gatcctaacg tccatttctc cggcgttttc ggcatgcctt cattgagcga tgaaaacggc
     2281 aattcactga ttggcagcct ggacattatg ggcgcgctga atcctgtagt cctgccaagc
     2341 gttctggcgc tggtgatgac ggcagtattt gatgccaccg gaactatccg tgccgtcgcc
     2401 ggccaggcga acctgctgga taaagatggg cagatcatcg acggtgggaa agcactgacc
     2461 actgactcca tgagcagcgt tttctctggc ctggtgggtg ctgctccggc agcggtatac
     2521 atcgagtctg cggcgggtac ggcggcgggc ggtaaaaccg gtttgacggc tatcaccgtt
     2581 ggcgtgctgt tcctgctgat tctgttcctc tctccgctct cttacctcgt tccggggtat
     2641 gcaacggctc cggcgctgat gtacgttggc ctgctgatgc tgagcaacgt ggcgaaaatc
     2701 gactttgctg attttgttga tgcgatggcg ggtctggtta cggcggtatt catcgtgctg
     2761 acctgtaaca tcgtaacagg catcatgatc ggcttcgcga ctctggtgat tggtcgtctg
     2821 gtttccggcg aatggcgcaa gttgaacatc ggtacggtcg ttatcgccgt ggcgctggtg
     2881 accttctatg cgggtggctg ggctatctaa tctttcatcg gattctgaaa acgggtggca
     2941 atggctgccc gtttttattt tctccgcaca ttgtgtgagc ttttgcgata ttctgaaaaa
     3001 atgagaattc aggcataacg cctttctcag atcataaaaa aacatcgcaa acagggaacg
     3061 catggaaatc ttcttcacca tactgataat gaccctcgtg gtctcgctgt ccggggtggt
     3121 cactcgtgtc atgccctttc agatcccgct tccgcttatg caaatcgcca tcggtgcgct
     3181 actggcgtgg ccgacgtttg gtttgcatgt ggagtttgat cctgaactct ttttagtctt
     3241 gtttatcccg ccgttgctgt tcgctgatgg ctggaaaacg ccgacccgtg aatttctcga
     3301 acatggtcga gagattttcg gcctcgcgct ggcgctggtg gtggtcaccg tggtcggcat
     3361 tggcttcctt atttactggg tggtgccggg cattccgctg atccccgcct ttgcgctggc
     3421 ggcggtactt tctccgaccg atgctgtggc gctctccgga attgttggcg aagggcgcat
     3481 cccgaaaaaa atcatgggca ttttgcaggg cgaagcgttg atgaatgacg cctccggcct
     3541 ggtgtcgttg aagtttgccg tggcagtggc gatggggacg atgatcttca ccgtcggcgg
     3601 tgcgacggtc gaatttatga aagtagccat tggtggtatt cttgccggtt ttgtggtgag
     3661 ctggctgtat ggtcgctcgc tgcgattcct cagccgctgg ggcggtgatg aacccgcgac
     3721 gcagatcgtt ctgctgttct tgctgccatt cgcttcttat ctgattgccg aacatattgg
     3781 cgtttccggc atcctcgctg cggttgccgc cgggatgacc atcacccgct ccggtgtgat
     3841 gcgccgtgcg ccgctggcaa tgcgcctgcg cgcaaacagc acctgggcga tgctggaatt
     3901 tgtctttaac ggcatggtgt tcctgctgtt aggtctgcag ctgccgggta ttctggagac
     3961 gtcgctgatg gcggcagaaa tcgaccctaa cgtcgaaatc tggatgctgt ttaccgatat
     4021 tattctgata tatgcggcgc tgatgctggt ccgtttcggc tggctgtgga cgatgaaaaa
     4081 gttcagcaac cgcttcctga agaagaagcc gatggagttt ggttcgtgga ccacacgaga
     4141 aatcctgatc gcgtctttcg ccggggtgcg tggggcgatc actctggccg gtgtgctctc
     4201 tatcccgctg ctcttgccgg atggtaacgt cttcccggcg cgctatgagc tggtgttcct
     4261 ggcggctggc gtcattctct tctcgctgtt tgtcggtgtg gtgatgttgc ctattctgct
     4321 acaacacatt gaagtcgcgg atcattcgca acaattgaaa gaggaacgta ttgcgcgagc
     4381 ggcaacggca gaagtggcga ttgtggcgat ccagaaaatg gaggagcgtc tggcggcgga
     4441 taccgaagag aatatcgata accagctgct tacggaggtc agttctcgcg tcattggtaa
     4501 cctgcgtcgt cgcgccgatg gacgtaacga cgttgaaagt tccgtgcagg aagagaacct
     4561 tgagcgtcgc ttccgtctgg cggcattgcg ttctgaacgt gctgaactgt accacctgcg
     4621 cgccacgcgg gagatcagca acgaaacgct gcaaaaatta ctgcacgatc tcgatttgct
     4681 tgaagcgttg ctaattgagg aaaatcagta agccgaaagc gacgtaacca ggcccgaggt
     4741 aatctgaaaa aggtgcaatc ttttgcacct ttaacaggtt agctcgggct aacattcatg
     4801 attctaaaac aaataagtta tctctcttgt aaagtcaatt cattggctgt tcttcgactt
     4861 aacctcaatc tgcgtgttgc ataatatctg cagtaatctt catagcgact gcattaatat
     4921 tatcctcagt ttgtgacatg ccagtatgag aggtacaatc ttcttcaaag aatttctgta
     4981 gtgttaccag ggtatcacta tcaatgggga tattttcctc tccacggagt atggaaatgc
     5041 ccattccctg gacgctagcc tcatcaaaat taacccgata gagacgggta ttaaacat
//
LOCUS       DAJTYD010000216         4944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTYD010000216 DAJTYD010000000
VERSION     DAJTYD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.219"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..237)
                     /locus_tag="OO585_003670"
     CDS             complement(<1..237)
                     /locus_tag="OO585_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCR6631113.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARF"
     gene            495..1274
                     /gene="deoC"
                     /locus_tag="OO585_003671"
     CDS             495..1274
                     /gene="deoC"
                     /locus_tag="OO585_003671"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6631114.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1401..2723
                     /gene="deoA"
                     /locus_tag="OO585_003672"
     CDS             1401..2723
                     /gene="deoA"
                     /locus_tag="OO585_003672"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6631115.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2775..3998
                     /gene="deoB"
                     /locus_tag="OO585_003673"
     CDS             2775..3998
                     /gene="deoB"
                     /locus_tag="OO585_003673"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6631116.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4078..4797
                     /gene="deoD"
                     /locus_tag="OO585_003674"
     CDS             4078..4797
                     /gene="deoD"
                     /locus_tag="OO585_003674"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6631117.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1191 a         1259 c         1359 g         1135 t
ORIGIN      
        1 aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac cttcaaacat
       61 atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct gcggataagg
      121 cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc taagcactgg
      181 gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag tgggcatctt
      241 cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat gttcactttg
      301 atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt tgaaccagat
      361 cgcattacag tgatgcaaac ttgtaagtag atttccttaa ttgtgatgta tatcgaagtg
      421 tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta ttggcgacaa
      481 gccaggagaa tgacatgact gatctgaaag caagcagcct gcgtgcactg aaattgatgg
      541 acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg tgtcatcaga
      601 ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt atcccgattg
      661 ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg gtaaccaact
      721 tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg gcaatcgcct
      781 acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg ggtaacgagc
      841 aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg aatgtactgc
      901 tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt aaagcgtctg
      961 aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa gtggcagtga
     1021 acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg ggcgtagaaa
     1081 aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg cagaaatatc
     1141 tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac taccgctttg
     1201 gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac ggtaagagcg
     1261 ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca ggcctacgag
     1321 acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc gcctcatact
     1381 cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa aaacgtgatg
     1441 gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc gacaacacta
     1501 tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat atgacaatgc
     1561 ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc gactggaaaa
     1621 gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc ggcgatgtca
     1681 cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg atgatctccg
     1741 gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc cctggcttcg
     1801 acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc gtggcgatta
     1861 tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc cgtgatatta
     1921 ccgcaaccgt agactccatc ccgctgatca ctgcctcgat cctggcgaag aaactggcgg
     1981 aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt atgccgacct
     2041 acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc gctggcgtgc
     2101 gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca ggtaacgcgg
     2161 ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg cgtctgtttg
     2221 atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg aaagatgacg
     2281 ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca gaagtctttg
     2341 gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac gcgaagtatc
     2401 tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt gtcagtgaaa
     2461 tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt cgtcaggcat
     2521 ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc gaccaagtag
     2581 acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg caggaagcgg
     2641 cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc acaccaactg
     2701 tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt tgaagcactg
     2761 agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc ggcatcggcg
     2821 ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt catatcgcag
     2881 aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat ctgccaaatc
     2941 tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt ccggcgggaa
     3001 tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg tcatccggta
     3061 aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt gagtggggat
     3121 acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg gtcgaacgcg
     3181 ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt ctggatcagc
     3241 tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct gactccgtgt
     3301 tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa ctgtgcgaaa
     3361 tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc gctcgtccgt
     3421 ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac ctggctgttg
     3481 agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt caggtggttt
     3541 ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa gtgaaagcaa
     3601 ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg ggtgataaca
     3661 ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt cgcgacgtcg
     3721 ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg atgtctttgc
     3781 tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg acctggaccg
     3841 gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa gtaaaaccgg
     3901 gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca aaatattttg
     3961 gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg cttgatgccc
     4021 tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga taaaacaatg
     4081 gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt gatgccaggc
     4141 gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg tgaagtgaac
     4201 aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat ttccgtaatg
     4261 ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat caccgatttc
     4321 ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca cgtaaaactg
     4381 cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg catccgtttt
     4441 aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc ggtagacgcg
     4501 gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga cctgttctac
     4561 tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg cgtggaaatg
     4621 gaagcggctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct gaccatctgt
     4681 accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg ccagaccacc
     4741 ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa agagtaattg
     4801 tgtttcgctg aaaggcgatt gtcgtgtgaa gccggagtgg gagactgctc cggcttttta
     4861 gtatctattc atcaatagca actgatttat agatttacta ttatttttca tatggataga
     4921 aaataaaatc tattcaagta tcta
//
LOCUS       DAJTYD010000217         4926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTYD010000217 DAJTYD010000000
VERSION     DAJTYD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.220"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1487)
                     /gene="dhaR"
                     /locus_tag="OO585_003675"
     CDS             complement(<1..1487)
                     /gene="dhaR"
                     /locus_tag="OO585_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6631118.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLR"
     gene            1715..2824
                     /gene="dhaK"
                     /locus_tag="OO585_003676"
     CDS             1715..2824
                     /gene="dhaK"
                     /locus_tag="OO585_003676"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6631119.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            2796..3428
                     /gene="dhaL"
                     /locus_tag="OO585_003677"
     CDS             2796..3428
                     /gene="dhaL"
                     /locus_tag="OO585_003677"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6631120.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3439..4857
                     /gene="dhaM"
                     /locus_tag="OO585_003678"
     CDS             3439..4857
                     /gene="dhaM"
                     /locus_tag="OO585_003678"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6631121.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1236 a         1262 c         1243 g         1185 t
ORIGIN      
        1 cgcagaggcg ggatggtaat ttcaaatgca tgcagcgcgt aatacagctg gcgactaaaa
       61 cgattttgtt ccaccagcat tgcgaggtcc gcggtcgttg ttgcaatcac tttgacatca
      121 attggtatta aacgccgcgc atccagtcgc gtgataacac cctgcttgat aacctgaagc
      181 aaagcagact gtaactccac cgccagatat tcaatctttt caagaaacag cgtgccgccg
      241 tgcgccagtt ccagccgact cagacggcca ttttcattgt ccgtacgatc gccaccaata
      301 aattcttccg ccagtgccgc atcaccatat aactcacaat tgacggcgat ataaggacct
      361 gcagcacgct cgctttcatt atgaattgcc tgacttagca gtgccttgcc cacgccctct
      421 tctccacaaa gcaggacagg aaagctactg cgcgccgcct ggcgaccaaa atgaatcaag
      481 cggcgggttt gcggatcgtc ctgtggcata tgagcaaagg tatggctgac ttttcctaat
      541 tgactggtca tcaactgccg catctgttcc acaggatgga gcaacaaaat aaagctggtt
      601 ccctgcgttt ctattatcgg tttaagggtt atcaccgcat caataaactg atgctgactt
      661 tcaaaggttg cttctacgtg tttgagcgga tgtgcctgtt ttattgcttg ttgcaatacg
      721 gcgggtaacg ttaagagttc agtgattgcc cgtccctgac ttgccgtcgc gtcaaggcgc
      781 aagacccgcg ccgcctgggc attgataaat tgcaaattac cctgctcgtc ccagctaatc
      841 acgccatcat ccatactttc taacagggca ttaagttgat ttaaatgacg gttagtttca
      901 gcgagcaaac tgtccgtcag cagtaaattt ccgacctcgc gggcgattgc caacgtcaac
      961 ggcaaatcag ctgcggtagt ttgctcaacc ggacacgcca gcgctattgt tcccgtcaat
     1021 cggcccttgc tgtcaaacaa cggcgttgca caaaaggccc agttccagag tgcctgtttg
     1081 aaatgttgat cggccatcgt tttcacggcc tgaccagaga tagccgctaa cgatagcgca
     1141 caagtaccaa taattccctc ggcgcaatac gtgccgtcat ggaatccaag tgcacttagc
     1201 tgctgcaagg tttgcggatc gccattacgg ctgagaatgc aggcggtttc atcgaggata
     1261 aacagcgcac actctcgcgg tgccatatat tcccaggcat cttccagcgc agcctgcccg
     1321 agcgtcagca tcgctttctt acgccgataa atagaagcaa atgtcacgcc ctgggcctga
     1381 tgtggtacgc tccatgtctc ccgtttcatc agcttattgc atcgctccca ggaggttgca
     1441 attaagggag atatgccccg accatcgttg ttaaaagcgc cactcatatc ccgcatccct
     1501 tatgacaaaa ggtgttccgg ctcgcaattt taagtattaa tacacgtaaa agtttcaata
     1561 tggaacattc attaaagcat atgtttcact atgaaacata atttgggcta attttctttt
     1621 cccttgcctg atgcacaatg gattcatcgg cagtcagtgg tcgccgtgtc gttgaacatc
     1681 atccatgccc taccgtaatt gctggagcaa aataatgaaa aaattgatca atgatgtgca
     1741 agacgtactg gacgaacaac tggcaggact ggcgaaagcg catccatcgc tgacactgca
     1801 tcaggatccg gtgtatgtca cccgagctga tgcccctgtt gcaggaaaag tcgccctgct
     1861 gtcgggtggc ggcagcggac acgagccgat gcactgtggg tatatcggtc aggggatgct
     1921 ttcgggggcc tgtccgggcg aaattttcac ctcaccgacg cccgataaaa tctttgaatg
     1981 cgccatgcaa gttgatggcg gcgaaggtgt actgttgatt atcaaaaatt acaccggcga
     2041 tattcttaac tttgaaacag cgaccgagtt actgcacgat agcggcgtaa aagtgaccac
     2101 tgtggtcatt gatgacgacg ttgcggtaaa agacagtctt tatactgccg ggcgacgcgg
     2161 cgttgccaac accgtattaa ttgaaaaact cgtaggcgca gcggcggagc gtggcgactc
     2221 actggacgcc tgtgcggaac tggggcgtaa gttgaataat caaggccact caataggtat
     2281 cgctctcggt gcctgtaccg ttcctgccgc gggcaaacct tcttttaccc tggcggataa
     2341 tgagatggag tttggcgtcg gcattcatgg tgagccgggt attgaccgcc gctccttctc
     2401 ttcccttgat caaaccgtcg atgaaatgtt cgacaccctg ctgggaaatg gctcatacca
     2461 tcgcacttta cgtttctggg attatcaaca aggcagttgg caggaagaac aacaaaccaa
     2521 acaaccgctc cagtctggcg atcgggtgat tgcgctggtt aacaatcttg gcgcaactcc
     2581 gctttctgag ctgtacggcg tctataaccg cctgaccaca cgttgccagc aagcgggatt
     2641 gactatcgaa cgtaatttaa ttggcgcgta ctgtacctca ctggatatga ccggtttctc
     2701 aatcacctta ctgaaagttg atgacgaaac gctggcactc tgggacgccc cggtccacac
     2761 cccggccctt aactgggata aattaggaga aagcaatgtc actgagcaga actcaaattg
     2821 ttaactggct cactcgttgt ggcgatattt tcagcaccga gagcgagtat cttaccggac
     2881 tggatcgcga aattggcgac gctgaccacg ggctaaatat gaaccgaggc tttagcaaag
     2941 tggtggaaaa actccctgct atcgcagata aagatatcgg tttcattctc aagaataccg
     3001 gtatgacgct gctttccagc gtcggtggtg ccagtggtcc gctgttcggt accttcttta
     3061 tccgcgccgc acaggcgacc caggcacggc aaagcctgac actggaagag ctttatcaga
     3121 tgttccgcga tggcgcggac ggcgtaatca gtcgcgggaa agccgaacct ggcgataaaa
     3181 ccatgtgtga tgtgtgggtg ccggtggtgg aatcgttacg tcagtccagc gagcaaaatc
     3241 tctctgttcc ggtggcgctc gaagctgcca gtagcatcgc cgaatccgct gcacaaagta
     3301 cgattacgat gcaagcccgc aaaggccgcg ccagttatct cggtgaacgc agtattggtc
     3361 accaggatcc cggcgcgacc tcggtgatgt ttatgatgca aatgttggcg ttagccgcaa
     3421 aagagtaagg aattggtgat ggtaaacctg gtcatagttt cacatagcag ccgactggga
     3481 gaaggtgtcg gtgaattagc ccgtcagatg ttaatgagtg atagttgtaa aatcgccatt
     3541 gccgcgggaa ttgacgatcc acaaaatcct attggtaccg atgccgtcaa agtgatggag
     3601 gccatcgaat ctgttgctga tgccgaccat gtgctggtca tgatggatat gggtagcgca
     3661 ttattgagtg ctgaaactgc gctggaattg ctggctccca agatcgccgc aaaagtacgt
     3721 ttgtgtgctg cgccgttggt cgaaggtaca ctggcagcaa cggtcagcgc ggcctcggga
     3781 gcggatatcg acaaagttat ctttgacgct atgcatgcgc tggaagccaa acgtgaacaa
     3841 ctgggtttac cgtcctccga cactgaaatc tctgacacat gtcctgcgta cgatgaagaa
     3901 gcccgttctc tggcggtggt cataaaaaac cgtaacggcc tgcatgtacg tccggcctcc
     3961 cggctggttt ataccttatc gacatttaat gccgatatgt tgctggaaaa aaacggcaaa
     4021 tgcgtcacac cagagagtat taaccagatt gcgttactac aagttcgcta taacgatacg
     4081 ctgcgcctga ttgcgaaagg gccagaagct gaagaggcac tgatcgcttt ccgtcagctg
     4141 gctgaagata actttggtga aacggaggaa gtcgctccac ctactctgcg tcccgttccg
     4201 cctgtttcgg gtaaagcctt ttattatcaa ccagttttat atacggtaca ggcaaaatca
     4261 accctgaccg tggaagaaga acaagatcga ttacgccagg ctattgactt cacgttatta
     4321 gatctgatga cgttaacagc gaaagcagaa gccagcgggc ttgactatat tgccgcaatc
     4381 ttttctggtc accatacact gttagatgat ccggaactgc tggcggcggc aagcgaactc
     4441 cttcagcatg aacattgcac ggcagaatat gcctggcagc aagttcttaa agaacttagc
     4501 cagcaatacc agcaactgga tgatgaatat ctacaagctc gctatattga tgtggacgat
     4561 cttctgcatc gcaccctggt ccacctgacc caaacgaaag aagaactccc gcagtttaac
     4621 tcgccaacta ttctactggc ggagaacatt tatccttcca cagtactgca actggatccg
     4681 gcggttgtaa aaggtatctg ccttagcgcc ggaagtccgg tatcccacag cgccctaatc
     4741 gcccgtgaac tggggattgg ctggatttgc cagcagggtg agaaactgta tgcgatacaa
     4801 ccagaagaaa cgctaacgct ggacgttaaa acgcaacgtt tcaaccgtca gggttaagcc
     4861 actgttcgga tggcatcgtt ctgatgtcat ccggcaattt actcagcact aatggttttt
     4921 aactac
//
LOCUS       DAJTYD010000218         4904 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTYD010000218 DAJTYD010000000
VERSION     DAJTYD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.221"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            718..2571
                     /locus_tag="OO585_003679"
     CDS             718..2571
                     /locus_tag="OO585_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6631122.1"
                     /translation="MAFKHYDVVRAASPSDLAKQLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTVGLNALTLTDITKNATYGVEIESLVLEINAPASS"
     gene            2721..2936
                     /locus_tag="OO585_003680"
     CDS             2721..2936
                     /locus_tag="OO585_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6631123.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2941..3291
                     /locus_tag="OO585_003681"
     CDS             2941..3291
                     /locus_tag="OO585_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6631124.1"
                     /translation="MTQNYKLIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            3355..3888
                     /locus_tag="OO585_003682"
     CDS             3355..3888
                     /locus_tag="OO585_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6631125.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            4187..4624
                     /locus_tag="OO585_003683"
     CDS             4187..4624
                     /locus_tag="OO585_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6631126.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GNGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
BASE COUNT         1263 a         1159 c         1348 g         1134 t
ORIGIN      
        1 ctgtgccgga tacatcacag tttctggaac cttaggtact acagtatcag tcagggtgct
       61 atattttcag atgtgatgaa agcctgtcag caggcagggc gtatcggaaa tgacccagta
      121 gagaaaacgt tgactcagat accggtgctg agttaccggg aaaccggcat cacatgaccg
      181 ctatccttcc aggcccatcc gctccggtgg gcctttttac tgcagaaaac aggttccccg
      241 ttaaatgcta tgttgctcac aattcagtaa gttgacagtt gcctgtcaga ctgggcattt
      301 gttaaaaaaa tttcgcatgg tgaatccccc tgagcggagg ggcgactggt gacggtataa
      361 tctctgatta tcaaaacgag aatgacgcgg gtttagtggc accgggctga actcaccggg
      421 aggcacccgg caccatgtgc atgatgatac agatacgcgg ctttagcccc tctccggagg
      481 ggctttctta tggacaaaaa aagcccgcgc tgggagacgc gggcggcaag gaataaacaa
      541 taaaacgtga agtaatattt cagctggcga ataatacccc atagtaatca ctctgcgcaa
      601 ctgcgcggtc tttttcgaat tgcgggctgt agtctccctt ctgccattgt cctgtaactt
      661 ccggacttca gcctgctcct tatctgactc acaacattat cccgcccggg aggattcatg
      721 gcatttaaac actatgacgt ggtcagggcg gcatcgccgt cagaccttgc gaaacaactg
      781 acacaaaaac tgaaggaggg ctggcagccg tttggtagtc cggtggccat aaccccttat
      841 accctgatgc aggcgattgc agcagaaggt gatgtggtcg tcagtggtgc aactgagccg
      901 gagtggtact acgtcatcgt actggccggg caatccaatg ccatggctta cggtgaaggg
      961 cttccgcttc cggattcata cgatgcgccc catccgcgca ttaagcaact ggcccgtcgt
     1021 aacacagtga ctcccggtgg tgaagtatgc gtatttaacg acatcattcc tgctgaccat
     1081 tgtctgcatg atgttcagga tatgagtacg attaaccatc cccgggctga cctgagcaaa
     1141 gggcagtacg gctgtgtcgg acagggctta catattgcca aaaaactgct tccgtatatc
     1201 cctaataatg cggggatcct gctggtacca tgctgtcgtg gtggttcggc attcacccag
     1261 ggcacggagg ggacattcag cgagtccacg ggagccagtc aggattcggc tcgctgggga
     1321 gtgggtaagc cgttatatca ggatctgctt ttccgcacga aggcagcatt gcagaaaaac
     1381 ccgaaaaacg ttttgctggc gatatgctgg atgcaggggg aattcgatat gacgaatgcc
     1441 agttacgccc agcagccagc agcatttctt gcaatggtac agcagttccg tgctgacctt
     1501 gccgggctgg cagcgcagtg tcacggtgga agtccggcat cagtcccctg gatttgtggc
     1561 gacacgacat acgcgtggaa acaagaacac ggtacgcaat atgaagtggt atatggtgca
     1621 tataaaggta aagaatccca gcagatttat tttgttccct ttatgaccga tggtagcgga
     1681 gttaatacac cgacaaacaa cccgtcagaa gatcctgata ttgccgggtc tggttattac
     1741 ggttcggcat cccgaacgaa caaaaactgg gtatcatcaa atcgcccgac gcatttcagc
     1801 tcatgggcgc gtcgtggcat tattcccgat cgtatggcaa ctgctattct gaacgtagcc
     1861 ggtcgcacct tagccttcat tagtggtaag gcaccggaaa tcaaaccctc gcccggcggc
     1921 gacacgccat cggggccgtc tgaagatgca tccgtacgca caatctccct gttgccgaca
     1981 gccggagatg ctgctgcgca gggctggagc attaagaatg gcggaattca gttgtcagat
     2041 ggtgtattta agatcaccaa gcagagcaat aaagcctggt ccctgacgcg cccggtggat
     2101 gacgcagtct ccctgctgac acggggtggc agactgagct gtaagtttcg actgtcaggc
     2161 gcactgacca acaaccagtt cggtctggga atttatctgt ataccgatgt agcgttacct
     2221 gacgtcgtgg cgatgaccgg gactggtaac ccgttcctga tgtcgttctt cacccagacc
     2281 acagacggca aactgaatct gatgcatcac aagaaagccg gaaacacaaa gttgggcgag
     2341 ttcgggaatt acagtaacga ctggcagacg ctggagctgg tgttcaccgc cggcagtgcc
     2401 acggttactc cgaaactgaa tggagtggct ggcccggcat tccaggtcat aaaagacagt
     2461 ctgacagtgg gactaaatgc actgacgctg acggatatta ccaaaaatgc aacgtatggc
     2521 gttgagatag aaagtctggt gctggagata aatgcaccgg catcatcata aaaagtgagc
     2581 cagtcaaatg gaaggtatcg ttaaactcac cggtagtgtc agtgggtcgt ctgagacgcc
     2641 tgcatgagtt atcagagcca tcagtactta actggtggct tttttattgt tgtcagcttc
     2701 cggataacgg gagacggggt atgtaccaga tggaaaaaat cacaacaggt gtgtcataca
     2761 ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg gtttccccgt
     2821 ctcagtgggc ggcaataggc gtgctgggga gtctgctgtt tgggctgctg acatatctga
     2881 ctaacctgta tttcaaaatc agagaggacc gtcgtaaggc tgcacgggga gagtaattca
     2941 atgactcaaa actataaact gattgtgaaa gggatccgca attttgagaa taaagttacg
     3001 gtaactttag cgttacggga caaaaaacgc tttgacggtg aaatttttga cctggacatc
     3061 tcgctggacc gtgttgaagg tgccgcgctg gagttttatg aggcagcagc cagaaggagc
     3121 atcagacagg tcttcctgga tgttgctgcc gggttatgtg aaggggatga gcagtcgccg
     3181 gaaaagcgcc ccgtaatttt agaggcgcag aatgtatgga taacctacaa aggaaagcta
     3241 ccgggaagaa ttactggttc tctgaagact cctccggaat cacaacctta agtcactgac
     3301 cggaacagat aaacctgtcc gtgggcagaa accgataaat cctgataaat atccatgaac
     3361 gcaaaaatca gatacggcct gtcggctgcc gttctggcgc tgattgccgc tggtgcgcct
     3421 gcgcctgaca ttctcgacca gtttctggat gaaaaggaag gtaaccacac cacggcatac
     3481 cgtgatggcg cgggtatctg gaccatttgc cgcggtgcca tcctggtgga tggcaaacct
     3541 gtcgttccgg gcatgaagtt gtcgaaggaa aaatgcgacc gggttaacgc cattgagcgt
     3601 gataaggcgc tggcatgggt ggagaaaaac atcagagtgc cattgagtga accccagaaa
     3661 gcggggatcg cgtcattctg tccgtacaac attggccccg gtaagtgttt tccgtcgacg
     3721 ttttataaac gaattaatgc aggtgatcgc aggggagcgt gtgaggcgat tcgctggtgg
     3781 attaaggacg gtggcagaga ctgccgtatt cgctcaaaca actgttacgg tcaggtatcc
     3841 cgtcgcgacc aggagagtgc gctggcgtgc tggggaattg acagataagc agaatatttt
     3901 gctgaaaaat gcggtttgct cacacggacg gataacacga aatcctgcga actgacaaaa
     3961 actaagagaa taaaagtaaa aaccccgttt gttggctgca agcggggttt tgtgtttcct
     4021 gactccggaa aagtcaaagg agaaagtgtg tttgatttta gcaaactgat tcgggagatt
     4081 cgagtgatgg ctgaaaaatt atccacctgg aagttcattc ttatctggct ggtgtttgtg
     4141 attatggcct ccggttattt catcggtcag atacgctggt ggtgaaatga accgcgtact
     4201 gtgcgtggtc atcattgccc tgctggtggc ctgtggtgcg cttagtctgg ggctgaatca
     4261 ttaccgtgat aacgccataa cctacaaaga gcagcgcgat aaagccacat ccacaatcgc
     4321 tgatatgcag aagcgtcaac gtgacgtagc agaactcgat gccagatata caaaggagct
     4381 tgctgatgct aacgcgacta tcgaaagcct ccgtgctgat gtttctgctg ggcgtaagcg
     4441 cctgcaagtc gccgctacct gtgcaaagtc aacgaccgga gccagcggca tgggcaatgg
     4501 agaaagccca agacttacag cagatgctga actcaattat taccgtctcc gaagtggaat
     4561 cgacaagata accgcgcagg tcaactacct gcaggagtac atcaggacgc agtgcttaaa
     4621 ataattttaa tttcactgaa atttaatacg tgactttcag gaaaatgcct cgcagatgcg
     4681 gggcattttt gtacaggtat ttcaccgcgc accgcagtgc actcaaccac gtcgaaccaa
     4741 accctttgga atgagccttt gagtagtcag ttagtgctgg tgagccttga cgggctgatc
     4801 tcctatgcag caaaggttca tctcaaagta aggcgaacgc tatgacaaac caagattcta
     4861 tagacctatc tgatcttcgt ggaatggtca gttttccaga caaa
//
LOCUS       DAJTYD010000219         4893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTYD010000219 DAJTYD010000000
VERSION     DAJTYD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.222"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..880
                     /locus_tag="OO585_003684"
     CDS             <1..880
                     /locus_tag="OO585_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6631127.1"
                     /translation="VVTSYAKAVDVKLAQWIEDNVTFPSTMVDRIVPAVTEDTLAKIE
                     QLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSH
                     SFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVDLQDYANRLIERY
                     SNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQ
                     GNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLS
                     LLAHGAKATVAKYSVK"
     gene            complement(916..1119)
                     /gene="ydfZ"
                     /locus_tag="OO585_003685"
     CDS             complement(916..1119)
                     /gene="ydfZ"
                     /locus_tag="OO585_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6631128.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1297..1981)
                     /gene="ydfH"
                     /locus_tag="OO585_003686"
                     /pseudo
     CDS             complement(1297..1981)
                     /gene="ydfH"
                     /locus_tag="OO585_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2070..2816)
                     /gene="ydfG"
                     /locus_tag="OO585_003687"
     CDS             complement(2070..2816)
                     /gene="ydfG"
                     /locus_tag="OO585_003687"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6631129.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            2953..>4893
                     /gene="dcp"
                     /locus_tag="OO585_003688"
     CDS             2953..>4893
                     /gene="dcp"
                     /locus_tag="OO585_003688"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6631130.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAI"
BASE COUNT         1213 a         1156 c         1276 g         1248 t
ORIGIN      
        1 cgttgtcact tcatacgcga aagctgttga tgtaaaactg gcacaatgga tcgaagataa
       61 cgtgactttc ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct
      121 ggcgaaaatc gaacaactta ccggtgtgcg cgatcctgcg ggcgttgcct gtgaaccttt
      181 ccgccagtgg gtaatagaag ataactttgt tgccggacgt ccagaatggg aaaaagcggg
      241 agccgaactg gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg
      301 cagtcattca ttcctggcgt atttgggtta tcttgccgga tatcagcaca ttaatgactg
      361 catggaagat gaacattatc gttatgcggc gtatggcttg atgttgcagg aacaagcgcc
      421 gacgctgaaa gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgaacgcta
      481 tagcaatccg gcgttacgtc atcgaacctg gcagattgcg atggatggta gccagaaatt
      541 gccacagcgg atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct
      601 gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa
      661 tccgatagaa atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc
      721 cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtgatgattt
      781 gccagacaat agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca
      841 tggcgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg
      901 gcggcattca cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc
      961 ttcacaacct tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc
     1021 agtatcaatc gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc
     1081 agtggtgatt gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat
     1141 ttacagtcga agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt
     1201 ttgtttttga tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa
     1261 aagcctctca tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct
     1321 gttttcctgg cggatctggc cacggattcg ctgatttcct gcaaatgttg cgtcattgca
     1381 cgttctaccg catcgccatc acgtttttgc agggcagaga aaatatcaag atgctggcgt
     1441 aacagcattt ctggtggaga aacatggtcg aaactcatat agcgcacgcg atcaacggtc
     1501 gctttcaggt tctcaatggt atcccacgcc agttgacagt cggcaatctg cgtcaggagt
     1561 tgatggaagt tgtcatcaag ttcaaaaaat catccagttg cttgtgctca atggcaatgc
     1621 gttgctggtg aagattttgt tccagttggt agcactggct ttcggtaatc atgctcgccg
     1681 cccgacgcgc caccgcgcac tcaatggcct gacggataaa actgccgttg cgcacctggg
     1741 ccatggaaat tttgttgacg tagctgccac gctgtggacg aatttgaatc aggccgtttt
     1801 ccgccagttt aataaaggct tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa
     1861 cttctttttc cgacaacggt gtgcctggag caatcaggca atggacaatg tcgcgacgaa
     1921 ggatacgata aatttgctga ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca
     1981 ttcgttcttg cttgtgagtg agttaactgc atgagtctac cacttttttg tgacaagcaa
     2041 taacccggca gttacgccgg gtataaaaat tactgacggt ggacattcag tccggcatag
     2101 ctttgggtaa ccggcatcat ttccagggta ttgatattga cgtgagcagg cagcgttgac
     2161 acccaccaaa cggcttcgct gacatcttct ggcgtcaatg caacggtatt ttgataggtt
     2221 ttttccgctt taccgtcatc gcctttaaag cggacattgg aaaactcggt gccacccacc
     2281 agacccggtt cgatgtcggt gacgcgcacc gccgtaccat gcaggtccgt acgcagattc
     2341 aggctaaact gacgaacaaa cgctttcgtc gcaccgtaaa cgttaccacc ggcataaggc
     2401 cagctacctg ccgttgagcc aatgttaata atatgaccat gattacgttc aaccataccc
     2461 ggtaagacgg cgcgcgtcat atataccagg cctttgttgt tggtatcaat catcgtttcc
     2521 cagtcttcaa cgctggcttt atgcgcaggc tccatgccca acgccaggcc ggcattattt
     2581 accaggatat caatattgca ccactcggca ggaagcgatg ccagcatctc ttcaatagcg
     2641 gcgcggttgc gaacgtccag ttgggcgatg tacagattat ctcccagttc gtcttttaac
     2701 tcctgcaacc gttcctggcg acggccagtg gcgataactt tatgcccttg ttgaataaaa
     2761 cgacgagtaa tgcattcacc aaaacctgcc gttgctccag ttactaaaac gatcatctca
     2821 ctgttcctca acgcttttgt gtgtcaataa catagcacgt gatgcttaag agcgggtaat
     2881 actggtttgt aagattgcac tccgttatca gtaagcctac gctatggaac acctcgcgtt
     2941 caggagaaga aaatgacaac aatgaatcct ttccttgtgc aaagcacact accgtatctg
     3001 gctccccatt ttgatcaaat tgccaatcat cactatcgcc cggcattcga tgagggaatg
     3061 cagcaaaagc gggcagaaat tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac
     3121 aatactattc tggcactgga acaaagcgga gaattactta cccgcgttac cagcgtcttt
     3181 tttgcgatga ctgcggcgca taccaatgat gaattacagc gtcttgatga acagttttcc
     3241 gctgaactgg cggaactggc taatgatatc tatctgaacg gtgaattatt cgcgcgggta
     3301 gatgctgtct ggcagcgccg tgaatccctg gggcttgata gtgaatccat ccgcctggtg
     3361 gaggtgattc atcaacgttt tgtccttgcc ggagccaaac ttgcgcaagc tgataaagca
     3421 aaattaaaag tactgaatac agaagctgcg accctgacca gccagtttaa ccagcgatta
     3481 ctggcagcaa ataaatccgg cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg
     3541 agtgagcaag agattgcgct ggcggcagag gcggctcgcg agaaaggtct ggataacaaa
     3601 tggctgattc cgctgctgaa taccacccaa caaccggcgc ttgccgagat gcgcgatcgt
     3661 gcgacgcgtg aaaaactgtt tattgcgggc tggacgcgag cggaaaaaaa tgatgccaat
     3721 gatacccgcg ctatcattca acgtctggta gagattcgcg cacagcaggc gaaactgctt
     3781 ggttttcctc attatgccgc atggaaaatc gccgatcaga tggcaaaaac gccagaagca
     3841 gcactcaact ttatgcggga aattgttcca gcggcgcgtc aacgtgctag cgatgaatta
     3901 gcctccatac aggcggttat cgataagcag caaggcgggt ttagcgcgca gccgtgggac
     3961 tgggcatttt atgccgaaca ggtacggcgg gagaaatttg atcttgatga ggcgcagctc
     4021 aagccatatt ttgaattaaa cacggtgttg aatgaaggtg tattctggac cgcgaatcag
     4081 ctcttcggta ttaagtttgt cgaacgtttt gatattcctg tctaccatcc tgacgttcgt
     4141 gtgtgggaaa tttttgatca taatggcgtg ggactggcgt tattttacgg tgatttcttc
     4201 gcccgtgatt caaaaagcgg cggtgcatgg atgggcaatt ttgttgagca atcaacgctt
     4261 aatgaaacgc atccggtaat ttataacgtt tgcaattatc agaaacccgc tgccggtgag
     4321 cctgcgttgt tactctggga tgatgtcata accttattcc atgaatttgg tcatacgctg
     4381 cacggccttt ttgcccgcca gcgttatgcc acgctttccg gcaccaacac gccgcgtgat
     4441 tttgtcgaat ttccgtcgca aatcaacgaa cactgggcaa cgcatccgca ggtattcgct
     4501 cgctacgccc ggcattatca gagcggggca gcaatgcctg acgaactgca acagaaaatg
     4561 cgtaatgcca gcctgttcaa caaagggtat gagatgagcg aactgcttag cgccgcactt
     4621 ctcgatatgc gctggcattg cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt
     4681 gaattgcggg cgctggtggc ggaaaatatg gatcttcctg ctataccgcc acgctatcgc
     4741 agcagttatt tcgcccatat ttttggtggc ggatatgctg caggttatta cgcttatctg
     4801 tggacgcaaa tgttggccga tgatggttac cagtggtttg ttgagcaggg cggattaacg
     4861 cgtgaaaatg ggcagcgttt tcgcgaggcg atc
//
LOCUS       DAJTYD010000220         4789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTYD010000220 DAJTYD010000000
VERSION     DAJTYD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.223"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            135..698
                     /gene="ahpC"
                     /locus_tag="OO585_003689"
     CDS             135..698
                     /gene="ahpC"
                     /locus_tag="OO585_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6631131.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAEIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            869..2434
                     /gene="ahpF"
                     /locus_tag="OO585_003690"
     CDS             869..2434
                     /gene="ahpF"
                     /locus_tag="OO585_003690"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6631132.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(2555..2983)
                     /gene="uspG"
                     /locus_tag="OO585_003691"
     CDS             complement(2555..2983)
                     /gene="uspG"
                     /locus_tag="OO585_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6631133.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3204..4442
                     /locus_tag="OO585_003692"
     CDS             3204..4442
                     /locus_tag="OO585_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6631134.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            complement(4673..>4789)
                     /locus_tag="OO585_003693"
     CDS             complement(4673..>4789)
                     /locus_tag="OO585_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Regulates the synthesis of nucleoside triphosphates
                     for nucleic acid synthesis, CTP for lipid synthesis, and
                     GTP for protein elongation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6631135.1"
                     /translation="LGLRVGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
BASE COUNT         1259 a         1147 c         1257 g         1126 t
ORIGIN      
        1 gcggtgcaaa gttcacaaag ttgtcttacg aaggttgtaa ggtaaaactt atcgatttga
       61 taatggaaac gcattagccg aatcggcaaa aattggttac cttacatctc atcgaaaaca
      121 cggaggaagt atagatgtcc ttgattaaca ccaaaattaa accttttaaa aaccaggcat
      181 tcaaaaacgg cgaattcatc gaaatcaccg aaaaagatac cgaaggccgc tggagcgtct
      241 tcttcttcta cccggctgac tttactttcg tatgcccgac cgaactgggt gacgttgctg
      301 accactacga agaactgcag aaactgggcg tagacgtata cgcagtatct accgatactc
      361 acttcaccca caaagcatgg cacagcagct ctgaaaccat cgctgaaatc aaatatgcga
      421 tgatcggcga cccgactggc gccctgaccc gtaacttcga caacatgcgt gaagatgaag
      481 gtctggctga ccgtgcgacc ttcgttgttg acccgcaggg tatcatccag gcaatcgaag
      541 ttaccgctga aggcattggc cgtgacgcgt ctgacctgct gcgtaaaatc aaagcagcac
      601 agtacgtagc ttctcaccca ggtgaagttt gcccggctaa atggaaagaa ggtgaagcaa
      661 ctctggctcc gtctctggac ctggttggta aaatctaaat ttccttcgtc tttcgcgcta
      721 ccaactccgg cgcgaaatac ttcggaaatt cacctaattc ttcgggtgct gcggcacccg
      781 attttcttcc ccgcactatg atgcaagctg catccaggta gccgtagagg ccgcttgcat
      841 gatgatgttt aaagcccagg agataaacat gctcgacaca aatatgaaaa ctcaactcaa
      901 ggcttacctt gagaaattga ccaagcctgt tgagttaatt gccacgctgg atgacagcgc
      961 taaatcggca gaaatcaagg aactgttggc tgaaatcgca gaactgtcag acaaagtcac
     1021 ctttaaagaa gataacagct tgccggtgcg taagccgtct ttcctgatca ccaacccagg
     1081 ttccaaccag gggccacgtt ttgcaggctc cccgctgggc cacgagttca cctcgctggt
     1141 actggcgttg ctgtggaccg gtggtcatcc gtcgaaagaa gcgcagtctc tgctggagca
     1201 gattcgccat attgacggtg attttgaatt cgaaacctat tactcgctct cttgccataa
     1261 ctgcccggac gtggtgcagg cgctgaacct gatgagcgta ctgaacccgc gcatcaagca
     1321 cactgcaatt gacggcggca ccttccagaa cgaaatcacc gatcgcaacg tgatgggcgt
     1381 tccggcagtg ttcgtaaacg ggaaagagtt tggtcagggc cgcatgacgt tgactgaaat
     1441 cgttgccaaa attgatacgg gcgcggaaaa acgtgcggca gaagagctga acaagcgtga
     1501 tgcttatgac gtattaatcg tcggttccgg cccggcgggt gcagcggcag caatttactc
     1561 cgcacgtaaa ggcatccgta ccggtctgat gggcgaacgt tttggtggtc agatcctcga
     1621 taccgttgat atcgaaaact acatttctgt accgaagact gaagggcaga agctggcagg
     1681 cgcactgaaa gttcacgttg atgaatacga cgttgatgtg atcgacagcc agagcgccag
     1741 caaactgatc ccagcagcag ttgaaggtgg tctgcatcag attgaaacag cttctggcgc
     1801 ggtactgaaa gcacgcagca ttatcgtggc gaccggtgca aaatggcgca acatgaacgt
     1861 tccgggcgaa gatcagtatc gcaccaaagg cgtgacctac tgcccgcact gcgacggccc
     1921 gctgtttaaa ggtaaacgcg tagcggttat cggcggcggt aactccggcg tggaagcggc
     1981 aattgacctg gcgggtatcg ttgagcacgt aacgctgctg gaatttgcgc cagaaatgaa
     2041 agccgaccag gttctgcagg acaaactgcg cagcctgaaa aacgtcgaca ttattctgaa
     2101 tgcgcaaacc acggaagtga aaggcgacgg cagcaaagtc gttggtctgg aatatcgaga
     2161 tcgtgtcagc ggcgatattc acaacatcga actggccggt attttcgtgc agattggtct
     2221 gctgccgaac accaactggc tcgaaggcgc agtcgaacgt aaccgcatgg gtgagattat
     2281 cattgatgcg aaatgcgaaa ccaacgtcaa aggcgtattc gcagcgggtg actgtacgac
     2341 ggttccgtac aagcagatca tcatcgccac tggcgaaggt gccaaagcct ctctgagtgc
     2401 ttttgactac ctgattcgca ccaaaactgc ataagaagaa gtaagattca cctgcaattg
     2461 cttagccgcc ggggtcaaac ctagcggctt ttttatggca ttaaaaagcc ctgccgggat
     2521 gttccaggca gggcggcata aatactttta tctgttagcg aacgaccagt acaggcagat
     2581 tggcgtggcg gatcacgctc gaggcgttag aacctaacag atgggtcgaa atcgatgggt
     2641 tgcgagaacc gataactaca acatcagccc ccagttcttc tgccaactca ttgacttcat
     2701 cccgcacgct accaaaacgg acatgttgtt taatgcggga aggatcgatg gtgaagtggc
     2761 tgaccatcgt ttgcagacgt tcttctgctt catgttgcag atgctcttca aaacgacgca
     2821 catcagcggc aaaacggtcc aggctcaggc tggctgaccc gggtagtacg tgaagtagat
     2881 gaataactcc gtcatcctgg gtgaggaatt cagcgtggcg aacagctttg tcgctcaatt
     2941 ccatttcaaa tacatcaact ggcataatga ttgtcttata cataaccctt tctccctgtt
     3001 aatcatgaac aaatcattcg ccatgattat aatatttatc cctgatattt gtctggttct
     3061 ttttccttac gaacggtttc tgtgattaat atattctcac tgaacaccag gaattctccc
     3121 aaaacctgcg gtaccgcccg ttttcccgct gtgatagcta cccttaaaga ctgactcttt
     3181 tttgaactgt ctctggaggt tgcatgaaag cattgactta tcacggccca catcacgttc
     3241 aggtagaaaa tgttcccgat ccgggcattg aacaggcaga tgatattatt ctgcgtatta
     3301 cggcaacggc gatctgtggc tctgatctcc atctttatcg aggcaaaata ccccaggtta
     3361 aacatggcga tatttttggt catgaattta tgggggaagt cgttgaaacc ggaaaggacg
     3421 taaaaaattt gcaaaaaggc gaccgggtgg taattccgtt tgtcattgct tgtggcgact
     3481 gttttttctg tcgaatgcag caatatgccg cctgcgaaaa taccaatgcg ggtaaaggcg
     3541 ctgcgctcaa taaaaaacag ataccagctc ccgcggcatt gtttggttat agtcacctgt
     3601 atggtggcgt tcctggtgga caggcggaat atgtccgcgt ccctaaaggg aatgtggggc
     3661 cgtttaaagt accgcctttg ctttcagatg ataaagccct tttcctttct gatattctgc
     3721 caacggcatg gcaggcagca aaaaatgcgc agatccaaca aggttcaagc gttgcagtct
     3781 atggtgccgg tccagtggga ttgttgacaa tcgcctgtgc acggttgctc ggtgcggaac
     3841 agatttttgt tgttgatcat catccctacc gcttgagttt cgccgctgac cgctacggcg
     3901 cgatcccgat taatttcgat gaagatagcg atccggcaca gtcaattatt gaacaaacgg
     3961 caggtcaccg gggcgtggat gcagtaatag acgccgtcgg ttttgaagcg aaaggcagca
     4021 ccacggaaac ggtgctgact aacctgaaac tggagggcag cagcggtaaa gcgttgcgcc
     4081 agtgtattgc ggcggtcagg cgtggcggca ttgttagcgt accgggcgtc tacgctggat
     4141 ttattcacgg tttcctgttt ggtgacgcct ttgataaagg gttgacgttt aaaatgggac
     4201 agacccacgt tcacgcatgg ctgggagaat tattaccgtt aattgagaaa ggattactga
     4261 aaccagaaga aattgttacc cactatatgc cgtttgaaga ggccgcccgg ggatatgaga
     4321 ttttcgaaaa acgtgaagag gagtgccgta aggtgattct ggtacccggt gcacaaagcg
     4381 cagaggaggc gcagaaggca gtttcaggtc tggtgaatgc gatgccgggg ggaacaatat
     4441 gatcgtcagg agtggttttc gaggtaaagg acagccatga cgataatcgc cgccataatc
     4501 agaaatccta tcaggatgta aaatgcttct gccatggtta ttcccacaaa cgaaacggaa
     4561 taattttgca gcaaaagtga acagtgagaa ccaggaaaaa ttgctgattt tgcgtaaaga
     4621 ggatgcgagt gcatcctctg ggcaaagcga gttatcgctt gtgctgatgg gattaaagca
     4681 ggtagtcgcc agcagcttct ggctggtatt cgagttccag cacttcaagg tgggttgcaa
     4741 cgccgcccgg aagttcccag tgaatggaat cgccaacgcg cagccccag
//
LOCUS       DAJTYD010000221         4603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTYD010000221 DAJTYD010000000
VERSION     DAJTYD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.224"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..148
                     /locus_tag="OO585_003694"
     CDS             <1..148
                     /locus_tag="OO585_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6631136.1"
                     /translation="FGSWCADLNLHCITAEFPPISSDEASEKYLFAMANLLRWHPKDA
                     IRPS"
     gene            complement(123..1088)
                     /gene="ycjG"
                     /locus_tag="OO585_003695"
                     /pseudo
     CDS             complement(123..1088)
                     /gene="ycjG"
                     /locus_tag="OO585_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            1207..1713
                     /gene="tpx"
                     /locus_tag="OO585_003696"
     CDS             1207..1713
                     /gene="tpx"
                     /locus_tag="OO585_003696"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6631137.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(1757..3298)
                     /gene="tyrR"
                     /locus_tag="OO585_003697"
     CDS             complement(1757..3298)
                     /gene="tyrR"
                     /locus_tag="OO585_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6631138.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(3446..4507)
                     /locus_tag="OO585_003698"
     CDS             complement(3446..4507)
                     /locus_tag="OO585_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6631139.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(4504..>4603)
                     /locus_tag="OO585_003699"
     CDS             complement(4504..>4603)
                     /locus_tag="OO585_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6631140.1"
                     /translation="EAFRPQVMDVDKPLPHIRLDAALEFLIGDKLR"
BASE COUNT         1095 a         1332 c         1089 g         1087 t
ORIGIN      
        1 tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt ttccgcccat
       61 ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca
      121 tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc taccgccagc
      181 caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa agcggcgcta
      241 atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg tgcacgcgct
      301 tcagtcgcca gcgccagcgc ttccgtcaga ccaccggttt tatcgagctt aatgttaacc
      361 atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact ctcatcagca
      421 caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc aggaaggggt
      481 tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc caacccttat
      541 gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc tgtgcgaatt
      601 gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa tttcgcgcct
      661 gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat gactaccgtc
      721 tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag cgattgctgc
      781 tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc tgcgccggca
      841 gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga cacgacgctc
      901 ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg cgtgcattcg
      961 ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga
     1021 cttccccggg caatcacaaa cggggtatgt aagggctagg cttcctcaaa taccttaacg
     1081 gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg
     1141 ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta aacaggaaag
     1201 ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc caattccatc
     1261 ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct gtctgacgtc
     1321 accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag tattgatacc
     1381 ggtgtttgcg ccgcatcagt acgtaagttt aaccaactgg caaccgagat cgacaacacc
     1441 gttgtgctgt gtatttctgc cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa
     1501 ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct gcaagccttc
     1561 ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt
     1621 gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac cgagccggat
     1681 tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg tgttgcacca
     1741 tcaggcatat tcgcgcttac tcttcgttct tcttctggct taagccatat tcccgcaact
     1801 tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc gtgctgggat
     1861 aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt tcgtccagcg
     1921 aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa
     1981 tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatat atagcgttct
     2041 ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc aggtcagcgg
     2101 ccagtttcgg acgcggcacg ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta
     2161 acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc aacacgttca
     2221 gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc agattcttct
     2281 gcgtagcgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca acccgacgga
     2341 aagtgccatc attaagaaaa cgcagtaatt tcgcctgcat ccgtggtgac atttccccta
     2401 tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc ttcccttccg
     2461 gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca cagttcagcg
     2521 ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca aagagatctt
     2581 tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg
     2641 cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg aaggcgctga
     2701 cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat cgcaacatca
     2761 ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca ggcgtaatct
     2821 ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct tgcggttcgc
     2881 tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta tggttgcgca
     2941 ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata tccactttgc
     3001 ttttcatatc gacagagagc acaggttcgg gcaacgcctc cagtaacgcg ctcaacgcca
     3061 gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca cctgcaatac
     3121 ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca aaattgaggt
     3181 agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt agcacgagta
     3241 gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc agacgcatgg
     3301 gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt ggatagcagg
     3361 aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga
     3421 tatccggtca gcacccgata ttgcattatt tttcgctggg cgttttgcct ttttgcagcg
     3481 tttctttcac ctgaccgata agctgacgac ggaaatcccc gaggcgaggt ttgtcatcgt
     3541 caatccacgg cagcgggcgg caaagctcca tagctttaat cccgagtcgt gccgtcagaa
     3601 gtccagcacc aattccctga gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca
     3661 tccagtccat ccccacttcg cgcaccagtt cgctggctcc ggcaaaagcg atattcagca
     3721 ataccagctt aaacagacgc aaacggctgt aataccccag ttcaatgcca tacagcgtgg
     3781 cgatgcgatt aattaaacgc agattgcgcc aggcgataaa cgccatatcg accaacgcca
     3841 gcgggctgac cgcaatcatc aacgttgatt ccgccgccga acggcagatt tcgcgccgcg
     3901 cctgggcatc taaaactggc tggaccaaat gcgcatacaa actgaccact tcacggtcgt
     3961 tttgcgtttc atggattgag gcataccagc gttgcagcgc tggatgagac tgatcaatac
     4021 ccgcctgctg cgccagtttt tcgcaaaatg cgcggccttt gcccgtgccg tggctgtgca
     4081 acaaatcgcg cgcttcgtcg cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc
     4141 actctgttac cacagaacct acgccagcgc cgataatcaa tgccccagcg gcacatccac
     4201 ccagcgccac ccagtcctga gtttgccagg cattcattgt ccactgtaca ccctgcccga
     4261 caacgcttgc gccaaacaga gccagcccgc ccatcaccat tttgcgccac aggctgcgtt
     4321 tcggacgtaa cgctgcatcc attaccgctt caacttgccc ctcttcttca ggcgcttcat
     4381 cgagcgtggc cggggcaaaa ttttgcgcct gattttcgtc aaaggtctgc tgcgccctga
     4441 attttggatt ctgatcgacc tccagcggac cgtcgaaatc aatacgtggt tttaacggtt
     4501 cggtcatcgc aatttatctc ctattaaaaa ttccagcgcg gcatcaagac gaatatgcgg
     4561 cagcggtttg tcgacatcca tcacctgcgg gcgaaacgct tca
//
LOCUS       DAJTYD010000222         4499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTYD010000222 DAJTYD010000000
VERSION     DAJTYD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.225"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            4..546
                     /locus_tag="OO585_003700"
     CDS             4..546
                     /locus_tag="OO585_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6631141.1"
                     /translation="MATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQLHETLPDGAV
                     IHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGAS
                     MVLGGVAQMLAPKDRTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVLYGEMRVGSRVV
                     SQEISTADEGDGGQVVVIGR"
     gene            607..4005
                     /locus_tag="OO585_003701"
     CDS             607..4005
                     /locus_tag="OO585_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6631142.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRVLQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            4072..>4499
                     /locus_tag="OO585_003702"
     CDS             4072..>4499
                     /locus_tag="OO585_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6631143.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKH"
BASE COUNT         1052 a         1126 c         1393 g          928 t
ORIGIN      
        1 gcactggcca cacagctccc ggcgtttcgt cagaaactga gcgacggctg gtatcaggta
       61 cggattgccg ggcgggacgt cagcacgtcc gggttaacgg cgcagttaca tgagactctg
      121 cctgatggcg ctgtgattca tattgttccc agagtcgccg gggccaagtc aggtggcgta
      181 ttccagattg tcctgggggc tgccgccatt gccggatcat tctttaccgc cggagccacc
      241 cttgcagcat ggggggcagc cattggggcc ggtggtatga ccggcatcct gttttctctc
      301 ggtgccagta tggtgctcgg tggtgtggcg cagatgctgg caccgaaaga cagaactccc
      361 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
      421 gttgcccagg gcaatgttct gccggttctg tacggtgaaa tgcgcgtggg gtcacgcgtg
      481 gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt ggtgattggt
      541 cgctgatgca aaatgtttta tgtgaaaccg cctccgggcg gttttatcgt ttatggagca
      601 tgacgaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
      661 aagtccacgc agctgctgag tgtgatcgat gccatcagcg aagggccggt tgaaggtccg
      721 gtggatggat taaaaagcgt gctgctgaac agtacgccgg tgctggacag tgaggggaat
      781 accaatatat ccggcgtcac ggtggtgttc cgggccggtg agcaggagca gacaccgccg
      841 gagggatttg aatcctccgg ttccgagacg gtgctcggta cagaagtgaa atatgacacg
      901 ccgatcaccc gcaccattac gtctgcaaac atcgaccgtc tgcgctttac cttcggtgta
      961 caggcactgg tggaaaccac ctcaaagggg gacaggaatc catcggaagt ccgcctgctg
     1021 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggtaaa
     1081 accacttcac agtatctggc ctcggtggtg gtggataacc tgccgccgcg cccgttcagt
     1141 atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa caaaacgctc
     1201 tggtcgtcat acaccgaaat tatcgatgtg aaacagtgct acccgaacac ggcactggtc
     1261 ggcgtgcagg tggactcgga gcagttcggc agccagaagg tgagccgtaa ttatcatctg
     1321 cgcgggcgtg ttctgcaggt gccgtcgaat tataacccgc agacgcggca atacagcggt
     1381 atctgggacg gaacgtttaa accggcatac agcaacaaca tggcctggtg tctgtgggat
     1441 atgctgaccc atccgcgcta cggcatgggg aaacgtcttg gtgcggcgga tgtggataaa
     1501 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg ctttggcggc
     1561 acagagccgc gcatcacctg taatgcctgg ctgaccacgc agcgtaaggc gtgggatgtg
     1621 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     1681 ttcgtgcagg accgaccatc agataaggtg tggacctata accgcagtaa tgtggtgatg
     1741 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgcc
     1801 gttgaggtga actggattga cccggataac ggctgggaga cggcgacaga gcttgtggag
     1861 gacacgcagg ccattctccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     1921 accagccggg ggcaggcaca ccgcgccggg ctgtggctga ttaaaacgga gctgctggag
     1981 acgcagaccg tggatttcag cgtcggcgca gaagggcttc gccatgtgcc gggtgatgtt
     2041 attgaaatct gcgatgatga ctatgcgggg atcagcatcg gcgggcgcgt gctggcggtg
     2101 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccatc ctccggtacc
     2161 acgctgataa gcctggttga cggaagtggc aatccggtca gcgtggaggt ccagtccgtc
     2221 accgacggcg tgaaggtaaa agtgagccgt gttcctgacg gcgttgccgg atacagcgta
     2281 tgggggctga agttgccgac gttgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     2341 aacgatgacg gcacgtatgc catcaccgcc gtgcagcatg taccggaaaa agaggccatc
     2401 gtggataacg gggcgcactt tgacggcgac cagagcggca cggtgaatgg tgtcacgccg
     2461 ccagcagtgc agcatctgac cgccgaagtc actgcagaca gcggggaata tcaggtgctg
     2521 gcgcgatggg acacgccgaa ggtggtgaag ggcgtgagct tcctgcttcg cctgaccgtg
     2581 gcagcggatg acggcagtga gcggctggtc agcacggccc ggacggcgga aaccacatac
     2641 cgcttcacgc agctggcgct gggacggtac acgctgacag tccgggcggt aaatgcgtgg
     2701 ggacagcagg gcgatccggc gtcggtatcg ttcaggattg ccgcaccggt agcaccgtcg
     2761 cggattgagc tgacgccggg ctattttcag ataactgcca cgccgcatct tgcggtttat
     2821 gatccgacgg tacagtttga gttctggttc tcggaaacgc ggattaccga tatcaggcag
     2881 gttgaaacca cagcccgcta ccttggcgcg gggctgtact ggatagccgc cagtatcaat
     2941 atcaaaccgg gccatgatta ttacttttat atccgcagtg tgaacaccgt tggcaaatcg
     3001 gcattcgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttacct ggattttttc
     3061 aaaggcaaga taaccgaatc ccatctcggt aaagagctgc tggaaaaagt tgatctgacg
     3121 gaggataacg ccagcagact ggatgagttt tcgaaagagt ggaaggacgc taacgataaa
     3181 tggaatgcga tgtggggcgt caaaattgag cagaccaaag acggcaaaca ttatgtcgcg
     3241 ggtattggcc tcagcatgga ggacacggag gaaggcaagc tgagccagtt tctggttgcc
     3301 gctaaccgta tcgcgtttat tgacccggca aacgggaatg aaacgccgat gtttgtggcg
     3361 cagggcaacc agatattcat gaatgacgtg ttcctgaaac gcctgacggc tcccaccatt
     3421 accagcggtg gaaatccgcc ggcattttcc ctgacaccag acggaaagct gaccgctaaa
     3481 aatgcggata tcagcggtaa tgtgaatgca aattcaggga cgctcaacaa tgtcacgatt
     3541 aatgaaaact gtcagattaa ggggaaactg tcagccaatc agattgaagg cgatattgtc
     3601 aaaacggtca gcaagtcttt cccccgcacg aacagttatg ccagtggcac catcacggta
     3661 agaatcagtg atgatcagaa atttgaccgg caggtcatga taccgccagt gttattccgc
     3721 ggtggtaagc atgagaattt caacagtaat aaccaacagt catactggta ttcaacctgc
     3781 cggttaagag tgacccgcaa tggtcaggag atttttaatc agtccacgac ggatgctcag
     3841 ggcgtatttt cctcagttat agatatgcct gccggacagg ggacgctgac actgacattc
     3901 accgtatctt catcaggagc gaataactgg acaccaacaa ccagtatcag cgatctgctg
     3961 gttgtggtga tgaaaaaatc cacagcaggt atcagtatca gctgaatttt ataacccaga
     4021 acgggcgtca gaaatgacgc cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa
     4081 ctttatgccg ccattttgtc cgcagccatt tgtctggccg tatccggtgc gcctgcatgg
     4141 gcgtctgaac atcagtccac gctgagcgcg gggtatcttc atgcccggac gaacgttccc
     4201 ggcagtgatg atctgaacgg gattaacgtg aaataccgtt atgagtttac ggacacactg
     4261 gggatggtga cgtcgttcag ctatgcagga gacaagaatc gccagattac ccgttacagc
     4321 gatacccgct ggcatgaaga ttccgttcgt aaccgctggt tcagcgtaat ggcggggccg
     4381 tctgtgcgcg tgaatgaatg gttcagcgcg tatgcgatgg cgggtgtggc ttacagccgt
     4441 gtgtcgactt tctccgggga ttatctccgc gtaactgaca acaaggggaa aaagcacga
//
LOCUS       DAJTYD010000223         4498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTYD010000223 DAJTYD010000000
VERSION     DAJTYD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.226"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..495)
                     /gene="hyfE"
                     /locus_tag="OO585_003703"
     CDS             complement(<1..495)
                     /gene="hyfE"
                     /locus_tag="OO585_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6631144.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLV"
     gene            complement(507..1614)
                     /locus_tag="OO585_003704"
                     /pseudo
     CDS             complement(507..1614)
                     /locus_tag="OO585_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(1626..1736)
                     /locus_tag="OO585_003705"
     CDS             complement(1626..1736)
                     /locus_tag="OO585_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6631145.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(1753..2700)
                     /gene="hyfC"
                     /locus_tag="OO585_003706"
     CDS             complement(1753..2700)
                     /gene="hyfC"
                     /locus_tag="OO585_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6631146.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(2711..4498)
                     /gene="hyfB"
                     /locus_tag="OO585_003707"
                     /pseudo
     CDS             complement(2711..4498)
                     /gene="hyfB"
                     /locus_tag="OO585_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
BASE COUNT         1235 a         1282 c         1185 g          796 t
ORIGIN      
        1 caccagatgg gagccgtttt ccatcaggca gtaaccaaaa atttgccgca ggatattgcg
       61 ctggctgaca atgcacagca ggccaagcag aaaatgacct aacgctaccg ccagcgccgg
      121 tttcagcccg gtagccatcg gtagcttcac gggctgaacg acaaatgcgc aaagcaggac
      181 aattaacgcc gcgagcagtg ccatcattgc cggaccgaat aacgcttttt ccgggatgtt
      241 ctggggaata tttcgtgcag cgtaagtcat gattaacggt accagcagca ctttggtgat
      301 aaaggcgctg gcggaccaga tcagcagttg ctctgcggcg aacaggcacg agagagtggc
      361 gaaaatagac accagcacca gcgactggca ggcgtaaaat ccgcatgaca ggcgatagct
      421 tttgacgcta atcacaaaca gcgatgtcag catcatcagt cccgccagat tatttacgat
      481 catagaaccg atcattcatc atctccttac tgcaaccagg tcgcggcgat tacgctggaa
      541 atcagcgaca tcacaatcag tacaatcaac accaggcgca ttgaccctgg cagcggtgcg
      601 gcatcggcga cggcctcgct cggtttgcca ggcacaacgc gaccaaacca gcgaataaac
      661 caggcgaaac tggcgaccga ttcaatcatc agcagaatca tggcgggcag caggatccag
      721 tactccactg acaacgcaaa accggcggca aacagcggga atttactgaa gaagccgttg
      781 aacggcggca caccggtaat cgccagcgct gccacgcaga aacccacgcc tggcaacggc
      841 agggagtgca atacgccacg cagacgcggc aacaagcgcg tgccgcagct gtaactcagc
      901 gcacctgcta caaggaaaaa caggctttta gcgaacgcgt ggttgacgat gtaggcgatg
      961 ctgccctcca gtgccagccg cccactttca ccatcgatgc ggcgtggaga taggctctga
     1021 tcggtgttgg cgcttccatt gcgtccggta gccacgcttg catcggtagc tgggccgatt
     1081 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccatgtaact
     1141 cgctcatcgc gctaagcaca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     1201 acaaccccaa cgaaccgata tgggtgataa gcaacgcttt tagtgttgaa cgctgcgctt
     1261 tatcgctctg gtaataactg atcaacgccc aggagcagcc gccctaattt caaaaaacaa
     1321 caactgaccg agcagcgtcg aggagagtac cagtcccgcc atcgcgccga taaacaccag
     1381 caaaaatgcg taataacgat tcgtgccgtt atgcgggtgt tcgcgatttt tatccgtcag
     1441 ataacccgtg gagtagatcg tgaccagcaa accgagaaac accaccacaa acagaatcag
     1501 cgtactcacg cgatcaatga ccaggccaaa caatgccaca tcccccacgt tgaccaacgt
     1561 caacgtgacg gcaactttat cggcctgata aaacgcggag atcagcgaca acatgcacag
     1621 cgtggtcagt gcggcgaaca aaacccccat tcggcggccc gacgttgtgg cgaaaacgaa
     1681 acgaccagtg cgccgataaa aggcagcatt aacgtcgtca gagcaagatt ttccatattc
     1741 cgtcagtgct ccttacagac cggttaacca gaagacccat gcaagcgcag caaggctgaa
     1801 gccaagccag gtcacatggt gaatgagtaa aaaacgcccg cgtgccagcg tgttttctgc
     1861 gattgaggcc agtacaaaaa tcagcaaaac cttgagcagc gtaacgacaa gtgaagtcag
     1921 caggcaggcg agagaaagtt cttgcgcgcg cccaaagggc agaaacaggg ccacaaacag
     1981 tgctgccatc acgacctgtt tcagccccag cccccatttc gctagcgcca gcccggcacc
     2041 ggaatattcg gtcagcgggc cttcctgtaa ttcctgttct gcttcagcaa catcaaaggg
     2101 aatttttccc atctcaatga agcaggcaaa accacaggcc agtaacgcca gtacggtggt
     2161 tagtggcgag ttccagcccg ttgccagcgt attgctgatc atctcgatat gcgtggaacc
     2221 tgctatcagc gccaatacca gcagtgagag aatgagcatg ggttcgacca gaatgccgag
     2281 cgtcaactcg cgactggcac cgactcccgc aaacgggctt ccggtatcca gtccggaaag
     2341 agcaaagaaa aaacgaaaca gggcaagaag atagataagg gtgatcagat cgccgccgcc
     2401 cgcaaaaggg gaaacggtaa taaaaagtgg tagggccatc gccagcacca gcatgctgct
     2461 gattaatacc cacggcatca ggcggaacat cagacctgaa gatataggcg caacttcctg
     2521 gcgtttaaac agtttgtgga tctcgcgata atcctgccag atccccggcc cgcggcggga
     2581 gtgcatacgc gcgcgtatct gccgggaaat acccgtaaaa agcggggtta gcagcagcag
     2641 aatcacggcc tgtgctaacg caaaaatgac cagatatccg tcgcaaagag tttgtctcat
     2701 ggtgatttcc ttagacggca atagcgatta gcagcacaac cagtgcggca actagataca
     2761 ggcaatagag acgaaagtcg ccgctttgca gatgctgtat ttctttggcc agccgttggg
     2821 tggcgctcac gatggggcgg atcacccgct catcccagaa gggttctgtg ctctgagccc
     2881 tggcggtgac gtgcgcaaga tctttattta gcctcagcgt agggtcgagc tgttgtcgta
     2941 gacgaaataa cgcagagaag accacacgca gcggctgcat cacgccattg cctgacggag
     3001 ccatcgcgtt ttcccagccg tagccgcatg cccaggcatc tcctgtgcga cgaaacgctg
     3061 cgcggcgcga acgacaccac agccagtaaa ggccaggcag caaaggcatc gccagtagta
     3121 acagcaacag taatgaagga gtgacccgtg tatgaaacgt gccgggtaca agtgctattc
     3181 cgctggcgac agtgacggga ggggtattgg taaacgcatg ggcaatatgc attatcttcg
     3241 gtgccagcca actggcacta agcgcaatta atacgcagag tgccgcgagc agtagcatgg
     3301 cgacgatcat cgtatttggc acttcctgtg cctcttcagc gtgtgtactg cgcggcgcac
     3361 cacagaaagt aataccgtac attttgacga agcacattac tgccagccca ccagtgactg
     3421 ccagcattac catagcaata ggacccgcaa gttgtagcgc tacggcttcc acacgactta
     3481 gtgagaacag cgactgccag gtgtaccatt cgctgataaa accattcagc ggaggaatgg
     3541 ctgatatcgc gaggcaacca atcaggcatg ctgcggctgt ccacggcatc cgtttcgcca
     3601 gtgcccccat tttttccatg tcgtgggtat gcaaacgcga aataatcgcg cccgcgccga
     3661 gaaatagcag ccctttgaac agcgcatggt tgagcagatg aaacagtgcg ccgagcagtc
     3721 caaccacggt gagcagcggg tcgtgcagtg acagaccgtc catcgccaca ccgactgcca
     3781 gcaaaataat gccgacgttt tcgacggtac tccaggccag cagccgtttg atatcctgtt
     3841 ccgccagcgc atatagcacg cccaggagcg cggagattgc gccgatcgcc atcaccagaa
     3901 tgccccacca cagaggcaaa cccgtttgcg ccagcagatc catcgctact ttcaggatgc
     3961 cgaaaatacc gattttgacc attacgccag acatcaacgc cgaagcgtgc gatggtgcgg
     4021 cagggtgagc gcgcggcaac cagctgtgca acggcatcat cccggctttc gcgccaaaac
     4081 cgaaaaaggc cagcagaaac atcgccgacg ccagccccgg agaaagtgaa agcgtgcgaa
     4141 actggcaaaa tcgaggctgc cgctttcgcg ccccatcagc aagaaggcga tcataatcag
     4201 caccgatccg gcgtgggcga taaaaaagta gagcatgcca gcgttgatcg acgttttatc
     4261 ctgcctggca atgaccagaa accaggaaga cagcgacatc atttcaaaca gcacgatgaa
     4321 ccaaaaagcg ttgtccatca ccagcagggc aaccatcgat gcgatgaaaa gattcataaa
     4381 gaagcccatc gccgccgcgc ctttgccctc gtattcgcgc atataagtca atgaatagag
     4441 cgaacaaacc accaccagca aggagataac cagcaccata aataccgaga ggctatcc
//
LOCUS       DAJTYD010000224         4404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTYD010000224 DAJTYD010000000
VERSION     DAJTYD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.227"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            228..600
                     /locus_tag="OO585_003708"
                     /pseudo
     CDS             228..600
                     /locus_tag="OO585_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1162..2109)
                     /gene="icsP"
                     /locus_tag="OO585_003709"
     CDS             complement(1162..2109)
                     /gene="icsP"
                     /locus_tag="OO585_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6631147.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGTHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3490..3642)
                     /locus_tag="OO585_003710"
                     /pseudo
     CDS             complement(3490..3642)
                     /locus_tag="OO585_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3698..4390
                     /gene="ospZ"
                     /locus_tag="OO585_003711"
     CDS             3698..4390
                     /gene="ospZ"
                     /locus_tag="OO585_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6631148.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1353 a          861 c          850 g         1340 t
ORIGIN      
        1 tgcgatctgg ttcaacaata tgcagatggt acacaacctc tttaaccagc ccatccggat
       61 tattcagagc ccttccgcaa tccggaataa aagtgctgtt ttaccgtgaa ccctctgaaa
      121 atcagcgagc agtgttttaa ccagccgacg ttcagagcgt actgacagac ggtggacaat
      181 ttgaataaac agttcaatca gaccgtagat gctctcccgt tggcgacatg ctgggagcgg
      241 tggaacaccg cttcggcaac gagcttccgg cgtctccagt gaagtgactg acggataatg
      301 gttcatgcta ccgggctaat gaaacacgcc agttcacccg gatgttggga ctttgttgaa
      361 ccgaagaaca cggcggtgcg gagtccggag agtaacagaa tagcaaagag cttcgtgaaa
      421 acaataaagt gtgactacat cagtatcatg cccaaacctg acgggttaac ggcagcaaag
      481 aaccttgcag aggcattcga gcattataac gaatggcatc cgcatagtgc gctggattat
      541 cgctcgccac gggaatatct gcggcagcgg gccaatgata acaggtgtct ggaaatatag
      601 gggcaaagcc acccacgaac gatccagcat ataatcaaga aattagattt catttaacat
      661 aagatagatt atgtgcacca agaggcattt ccttaatttc tgaatttaca ggctcttttg
      721 ctctctttgc tatgctgtgg atttggaaag atagagtgct ggctgattat ttaggtattt
      781 tcatcagcat gtccctgata gcactgttcc atcagcgctt tcaggcgata atagtagacc
      841 ttgggtagcc tctgctcatg tgtaacttga taccccgaga acagccacga tgatctaagc
      901 ggttcttatt tctccggtct cagggtcggt aatgccaaca gtaagtccac agtaatcaac
      961 gaagagtttt ttgaaccgcc ccggaaatcc tggagactaa acgccctgag aaagaggtaa
     1021 acagaatgac taaaaataca cgtttttccc ccgaagtccg tcaacgggca gttcgtatgg
     1081 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttttagc catgaaaacg
     1141 taatcaactc ggtgaaccaa ttcaaaaaat atactttata cctgcggaag tgagaaaacc
     1201 aatatattca atacttgcac tatttttaat ggtgccagat atattggtac tttgctcata
     1261 aagagatgta tcaccttttt tattagaaat gtaatttctg gagccttcta tataaaattt
     1321 tgcactgggg gtgacatagt atccaatatt cgcagcaaca gagcagtaat tttggttttt
     1381 tatttcatct ttaaaaattg tctcagtctg atagtgttta tctgtatcag atgaacgaac
     1441 ccaaccacta tatttcagtt ctgctccaaa ctcaaaattc tcatggcggt aattagcagt
     1501 taatccaata taaggtattt tgaagagctg tttgtaacct attgttcttt caccactagg
     1561 atgtgtccct tttttattcc tgttcccccc gttttcacta taaatataac tccctcccat
     1621 tgcattaaaa ctgtaacgac tctcttggta acctgctatt agtccaagtc gataatccaa
     1681 attatttaat aaccaacctt taagattcag ctcgaattcg ttagcatcac ggagatgcgt
     1741 attgggatgc cagctctggt cggtccatac ctgaggggta ttggaattgt tccagtcttt
     1801 atcaaccatg cttgctttct ggttacctaa agtagtccaa ccggaaactc cgaaagacac
     1861 ttttggaatt agcttccaat cgatgccacc tctaacaata gtcgcattac tgtatttcca
     1921 gtccagttga ctaatttttc tccccccttc cttgggatga taaacgcgtt cttttgtttt
     1981 gccactcaga gatcctagac taatatccgt actgatgtta tccggtatga aaagtggata
     2041 gttagtggta gcatgtgtag taaagatcgc aggtacacaa agtgcaagta caaggaattt
     2101 taatttcatc gagaactcta cttttttggt tgaaatgtcc atgatcttct tactttataa
     2161 gataaacgtc aatgccaagc attattttaa ttaaatattt gtttatgtta ccaagaacat
     2221 atcttttatt ataatattta ttgaactttt gtaaaaataa aaaaatacct cattgtgcga
     2281 ataaagtaac gggggccata gatttctcta cctacctttt ggtgttcata cctgaggctg
     2341 atagtttcag gattccgcga caattctact caccttcttg ttctctcgcc aatatgaaat
     2401 caatcagaaa agcgttccat aagaacaacg ataagacaat aaattgtcat cacggctgca
     2461 atagccaacg tgcctttcag taccaacatg acaacctcct gtatttgacg taacgtgtgc
     2521 tgataaatat gtatttgtat ctcaactgac tcaatacaac acttaagtat agtttttcag
     2581 attttgtttt tcccgtgaaa tattatggaa atggcagaag caatacattg aattccgatt
     2641 cgagtgaata cggctagcgg gcacctcact ttagcactga agccatcaac ttttatttga
     2701 cttatttcaa tggaaagctt tctcactatc agtacaggta attcccatcc ttttaaagtc
     2761 gagagtcaga ctgcgactaa tacagttaaa gtgaccaaac tcagttatcc tctacacatt
     2821 ttacctcatg tttacttgga caattcattg gtgcttttat aaaagaataa atattctact
     2881 aatttaatgc atcacctaaa atagtatttt ttataataga actgtatcta tagtatatta
     2941 ttaaagctta attatttatt atgctttaat gtggttgtcc gattaaggac taatccttga
     3001 aaacgccgta tagggcggga gttcactcta ttgcgataac gtaaccgtac aatattgtgc
     3061 gtttcccaaa ttgaccacta ctgaactatt ctgggattta aatgcaataa aatggttggt
     3121 tgaaggtcgt ggtgatgatg ttagtccttt tgagaatatt ccagtgccag atcttactta
     3181 taataaagag gcttggcagt ttggataagc tattgtcctt attccactaa tacaaacttt
     3241 ttgtaaagta tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa
     3301 acaattacac cataaaagga agagggagcc ccaattaagt tatcaagcat gtttatgata
     3361 cctgcagggt ataaattttc tggctgcaag cagacacata taaaacgtct ttattgatcc
     3421 tacccacata atatggacgc agtcgtaagt gaggctctgt gagcctaccc acgtaatatg
     3481 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc caccacacca
     3541 acggtgccgt cgccagcgat tgtaatcgca ctcgatataa ttaaacactg ttgtccgcac
     3601 tatttcccga tagataaagt gttctccgtg gagacattcc acttaggaac aattatattg
     3661 ttcatggtaa tgtttatatt tgagtataga gattaaaatg attagtccca tcaagaatat
     3721 taagaacgta tttccaataa atactgcaaa tactgaatat atagtgagaa atatttatcc
     3781 tagagttgaa catggttact ttaatgaatc accaaacata tatgacaaga agtatatctc
     3841 tggtataacc agaagtatgg cacaattaaa aatagaggag tttattaatg agaaatccag
     3901 acgacttaat tatatgaaga caatgtattc tccatgtccg gaagattttc agcctatttc
     3961 cagagacgaa gccagtatac cggaaggaag ctggttgaca gttatatctg gaaaacgtcc
     4021 tatggggcaa ttttctgtgg atagtttgta ccatcctgac ttacatgcat tatgtgagct
     4081 cccagagatt agttgtaaga tcttccctaa agaaaatagt gattttttgt atataattgt
     4141 tgtattcaga aatgattctc cccaaggaga actacgggca aatcggttta tagaactata
     4201 tgatataaaa agagagatta tgcaagtgtt aagagatgag tcgccagagt taaagtctat
     4261 taaatctgag attattattg ctcgtgaaat gggagaactt tttagttatg catcagagga
     4321 aatagatagc tacataaaac aaatgaatga caggttttct caaatcaagg ctcgaatgtc
     4381 tgttacttaa tgtatattaa ttgt
//
LOCUS       DAJTYD010000225         4314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTYD010000225 DAJTYD010000000
VERSION     DAJTYD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.229"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..1381
                     /gene="sad"
                     /locus_tag="OO585_003712"
     CDS             <1..1381
                     /gene="sad"
                     /locus_tag="OO585_003712"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR6631149.1"
                     /translation="TPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRDWRE
                     TNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAEHGP
                     AMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLKHAP
                     NVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGAAIG
                     AQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFIIEEG
                     IASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLLLGG
                     EKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFGLSA
                     TIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHEFCN
                     IQTVWKDRI"
     gene            1445..2371
                     /gene="glsB"
                     /locus_tag="OO585_003713"
     CDS             1445..2371
                     /gene="glsB"
                     /locus_tag="OO585_003713"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6631150.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            2371..2730
                     /locus_tag="OO585_003714"
     CDS             2371..2730
                     /locus_tag="OO585_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6631151.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2869..4287
                     /locus_tag="OO585_003715"
     CDS             2869..4287
                     /locus_tag="OO585_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6631152.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
BASE COUNT          973 a          969 c         1222 g         1150 t
ORIGIN      
        1 tactccggca actcatgcaa tttcgataaa tcctgccacg ggtgaacaac tttctgtgct
       61 gccgtgggct ggcgctaacg atatcgaaaa cgcacttcag ctggcggcag caggctttcg
      121 cgactggcgc gagacaaata tagattatcg tgctgaaaaa ctgcgtggta tcggtaaggc
      181 tctgcgcgcc cgtagcgaag aaatggcgca aatgatcacc cgtgaaatgg gcaaaccaat
      241 caatcaggcg cgcgctgaag tggcgaaatc ggcgaatttg tgtgactggt atgcagaaca
      301 tggtccggca atgctgaagg cggaacctac gctggtggaa aatcagcagg cagttattga
      361 gtatcgaccg ttggggacga ttctggcgat tatgccgtgg aactttccgt tatggcaggt
      421 gatgcgtggc gcggttccca tcattcttgc aggtaacggc tacttactta aacatgcgcc
      481 gaatgtgatg ggctgtgctc agctcattgc ccaggtgttt aaagatgcgg gaatcccgca
      541 aggcgtatat ggctggctga atgccgacaa cgacggtgtc agtcaaatga ttaaagattc
      601 gcgcattgct gctgtcacgg tgaccggaag tgttcgtgcg ggagcggcta ttggcgcaca
      661 ggctggagcg gcactgaaaa aatgcgtact ggaactgggc ggttcggatc catttattgt
      721 gcttaacgat gccgatctgg aactggcggt taaagcggcg gtagccggac gttatcagaa
      781 taccggacag gtttgtgcag cggcaaaacg ctttattatc gaagagggaa ttgcttctgc
      841 atttaccgaa cgttttgtgg cagctgcggc agccttgaaa atgggcgatc cccgtgatga
      901 agagaacgct ctcggaccaa tggctcgttt tgatttacgt gatgagctgc atcatcaggt
      961 ggagaaaacc ctggcgcagg gtgcgcgttt gttactgggc ggggaaaaga tggctggggc
     1021 aggtaactac tatccgccaa cggttctggc gaatgttacc ccagaaatga ccgcgtttcg
     1081 ggaagaaatg tttggccctg ttgcggcaat caccgttgcg aaagatgcag aacatgcgct
     1141 ggaactggct aatgatagtg agttcggcct ttcagcgacc atttttacca ccgacgaaac
     1201 acaggccaga cagatggcgg cacgtctgga atgcggtggg gtgtttatca atggttattg
     1261 tgccagcgac gcgcgagtgg cctttggtgg cgtgaaaaag agtggctttg gtcgtgagct
     1321 ttcccatttc ggcttacacg aattctgtaa tatccagacg gtgtggaaag accggatctg
     1381 acgcgacata ttcagctctg atatactcgc aggtcttttc agacctgcgg tccaggagta
     1441 gaaagtggca gtcgccatgg ataatgcaat tttagaaaac atcttgcggc aagtgcggcc
     1501 gctcattggt cagggtaaag tcgcggatta tattccggcg ctggctacag tagacggttc
     1561 ccgattgggc attgctatct gtaccgttga cggacagctt tttcaggccg gagacgcgca
     1621 agaacgtttt tccattcagt ctatttccaa agtgctgagt ctcgttgtcg ccatgcgtca
     1681 ttactccgaa gaggaaatct ggcaacgcgt cggcaaagat ccgtctggat caccgttcaa
     1741 ttctttagtg caactggaaa tggagcaggg tattccgcgt aatccgttta ttaatgctgg
     1801 tgcgctggtg gtctgcgata tgttgcaagg gcgattaagc gcaccacggc aacgtatgct
     1861 ggaagtcgtg cgcggcttaa gcggtgtgtc tgatatttca tacgatacgg tggtagcgcg
     1921 ttccgaattt gaacattccg cgcgaaatgc ggctatcgcc tggctgatga agtcgtttgg
     1981 caatttccat catgacgtga caaccgttct gcaaaactac tttcattact gcgctctgaa
     2041 aatgagctgt gtagagctgg cccggacgtt tgtctttctg gctaatcagg ggaaagctat
     2101 tcatattgat gaacctgtgg tgacgccaat gcaggcgcgg caaattaacg cgctgatggc
     2161 gaccagtggt atgtaccaga acgcggggga gtttgcctgg cgggtggggc taccggcgaa
     2221 atctggcgtt ggtggcggta ttgtggcgat tgttccgcat gaaatggcca tcgctgtctg
     2281 gagtccggaa ctggatgatg caggtaactc gcttgcgggt atcgccgtcc ttgaacaatt
     2341 gacgaaacag ttagggcgtt cggtttatta atgcagtctc tcgatccact cttcgcgcgt
     2401 ttatcccgtt caaaatttcg ctctcgcttt cgtctgggca tgaaagagcg tcagtattgc
     2461 ctggagaaag gcgcgccagt catcgaacaa catgcggcgg attttgtcgc taaacgtctt
     2521 gctccggcat taccggctaa tgacggtaag caaacaccca tgcgcgggca tccggtgttt
     2581 atcgctcagc acgccaccgc gacctgctgt cgcggttgtc tggctaagtg gcataacatt
     2641 ccgcagggtg tttcgttaag cgaagaacaa caacgttata tcgtggcggt aatctatcac
     2701 tggctggttg tacagatgaa tcagccatga gaattgcgga aaacgataaa atagcattcc
     2761 cagggtacct ttatttgccg ccgctgttag cgaacatgct atcctttatg agaatttact
     2821 tattacgttg agataattgc cacccgcaat tgtatggaca gataattaat gcatgtacac
     2881 cccatttcaa ctttccgttt gttccaggaa ggtcatctgc tacgtaatag catcgctatt
     2941 tttgcgctaa ccacgctgtt ctattttatt gttgcagagt tacggctggt tcacgaactt
     3001 tctcttttct ggccgctgaa tggcgtaatg gcgggggtgt ttgcccgcta tgtctggctt
     3061 aatcgactgc attactatgc gatcagttat gtggcgatgc tggtttatga tgccataacc
     3121 accgaatggg gactggtttc actggttatc aatttctcca atatgatgtt tattgttacc
     3181 gtcgccttac tggtcgtgcg ggataagcgt ctggggaaaa ataagtatga gccagtaagt
     3241 gccttacggc tatttaatta ctgtctgatt gccgcattat tatgcgctat tgtcggggcg
     3301 attggttcgg tcagtattga tagtctggat ttctggcctt tgcttgcgga ctggttcagt
     3361 gagcaattct caacgggcgt gttgatcgtg ccttgtatgc tgacgttggc tattcctgga
     3421 gtgctgccgc gctttaaagc agaacagatg atgcccgcca tcgcgcttat tgtgtcggtt
     3481 attgcctcgg tagtcattgg cggagcgggg agtctggcgt tcccgctgcc tgcattaatc
     3541 tggtgtgcag tgcgctatac gccgcaggta acatgtctgt tgacctttgt caccggtgcg
     3601 gtggaaatcg tactggtggc aaattcggtg attgatatct cggtcggttc gccgttctcc
     3661 attccggaaa tgttctccgc acgtctcggt attgccacga tggcgatatg cccaattatg
     3721 gtttctttta gcgtggcagc gatcaattcg ctaatgaagc aagttgcgct gcgagccgac
     3781 tttgattttc tgactcaggt ttactcacgg tccggtcttt atgaggcgct gaaaagtcca
     3841 tcgctgaaac agacacaaca tctgacggtc atgctgcttg atatcgacta tttcaaaagc
     3901 attaacgata actatggaca tgaatgtggc gataaagtgt taagcgtgtt tgcccggcat
     3961 attcagaaga ttgtcggtga taaggggctg gtggcgcgca tgggcggcga agaatttgct
     4021 gttgcagtgc cgtcggtgaa tcctgtcgat ggtctgctaa tggcggaaaa aatccgtaaa
     4081 ggcgttgaac tgcaaccatt cacctggcaa caaaaaacgc tttatctcac ggtaagtatt
     4141 ggcgtcggta gtggtcgcgc atcgtaccga acgctgaccg atgattttaa taaattgatg
     4201 gtcgaagccg atacgtgtct gtatcgctcg aagaaagatg ggcgcaaccg taccagcact
     4261 atgcgttacg gtgaggaagt tgtctgacat ctttattcat tccgcgcgat ttga
//
LOCUS       DAJTYD010000226         4294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTYD010000226 DAJTYD010000000
VERSION     DAJTYD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.230"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            140..586
                     /locus_tag="OO585_003716"
     CDS             140..586
                     /locus_tag="OO585_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6631153.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            597..2048
                     /locus_tag="OO585_003717"
     CDS             597..2048
                     /locus_tag="OO585_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6631154.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2038..3108
                     /locus_tag="OO585_003718"
     CDS             2038..3108
                     /locus_tag="OO585_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6631155.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            3108..>4294
                     /locus_tag="OO585_003719"
     CDS             3108..>4294
                     /locus_tag="OO585_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6631156.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLVLLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     VEP"
BASE COUNT          972 a         1104 c         1155 g         1063 t
ORIGIN      
        1 taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa attgtcattg
       61 tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg attaaaatat
      121 attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc tgaatattca
      181 gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac aaaacggttt
      241 tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa tgcactctac
      301 tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta aacaaccgtt
      361 cgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg gcgaagacgt
      421 caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg tcaaaatcat
      481 cggcacactg tgtcgcaaaa ttattcacaa ggaatttatt catcaactgc aacagggcga
      541 taccgaccaa gtgcttgcct tgttaaatca aaccctcagc tcataaggaa gtggcgatgg
      601 agtcatcctt acgtattgtc gcgatcacca actgccccgc cgggatcgct cacacctaca
      661 tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata aaagtggaaa
      721 ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc gctgccgatt
      781 acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg tttaccggga
      841 agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag attttcagcg
      901 aattaccgac aaactcgcag ctttttgccg cagatagtgg cgtgaagctt ggcaaacagg
      961 aaatgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg gcgctgccgt
     1021 ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag ttcggtttgc
     1081 cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctg ggtagttgaa tccatcggtt
     1141 accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg tcgattgccg
     1201 ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac aaagcgctgc
     1261 tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg gcgattggct
     1321 atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg ctgctcggtt
     1381 caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac tacgttatcg
     1441 ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc attcctccat
     1501 caatgaagtt tgcagcagcg tttctggtcg gtgcgatgct ggcgttcgat atgggcggcc
     1561 ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac atttatgact
     1621 ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt cttgcgacgt
     1681 tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt gccattgtgg
     1741 tcggtgccac agtcgcaacc gaaccggcta taccttacgc tctggcagct ccgttgccga
     1801 tgattaccgc caatacgctg gcgggtggca ttaccggcgt gctggtgatc gctttcggaa
     1861 tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg atgtcgccgg
     1921 tgggttcgtt ttatctggtg ctggcgattg gcctcgcgct gaacatttca ttcattatcg
     1981 tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag cttgtccatg
     2041 aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga tgaacaggaa
     2101 gttcgcgaca ttctgataaa cacgctggaa ccttgcgtta atgagatcac ctttgatggt
     2161 ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt tgtcgggcat
     2221 atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct gcgtttcacc
     2281 accattggtg gctggtggaa tcagtcgatg ctcaaccacc gggtaaccat acgcacacac
     2341 aagggagtga aaatccctgg tgtgattggt tccgtcgcgc ctcatgcgtt aacggaaaag
     2401 caaaggcaac ggccgctgtc atttgatgag atgttcattg atattggcgc aaacagtcgc
     2461 gatgaggtgg aaaaacgcgg tgtggcgatg ggcgatttta ttagcccgga agccaatttt
     2521 gcctgctggg gcgaagataa agtggtcggc aaggcgctgg ataaccgcat cggctgcgca
     2581 atgatggctg aactattgca gacggtgaat aatcccgaaa ttacgctgta tggcgtcggc
     2641 agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca cattaaaccg
     2701 gatgtagtga tcgtgctgga taccgctgtc gcaggcgatg ttccgggcat tgataacatt
     2761 aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa gcgctacttc
     2821 cccaaccaga aactggtagc agcgttaaaa agctgtgccg cacataacga tttaccgctg
     2881 caattttcca ccatgaaaac cggtgcgacg gatggcgggc gctacaacgt gatgggcggc
     2941 gggcgtccgg ttgttgcgct gtgcctgcca acccgctatc tgcacgctaa cagcggtatg
     3001 atttcaaaag ccgattacga tgctctgctc acgttgatac gggattttct gaccacctta
     3061 accgcggaga aagtcaacgc gtttagccag ttccgtcaga tggattaatg ctaaacgaac
     3121 gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggt gagctggcgc
     3181 aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc aactttaccc
     3241 ttaacggcaa agcccgcatt tccgccagtg gtagtgcggg ctatcagctg gaaatcttcg
     3301 agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgactatcgg ctgctggtac
     3361 tgttattact gaataccttt actccccgtg cgcaactcgc ctcggcgctt aatttgccag
     3421 aaacgtgggt cgcagagcgt ctgccccggt taaaacagcg ttatgaacgc gcttgttgcc
     3481 tggccagccg ccccggtttg ggccatttca ttgatgagac agaagagaag cgagttatct
     3541 tgctggcgaa cttgctgcgc aaagatccgt ttttaattcc gctggcgggc gtaacacgag
     3601 acaaccttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg ctcatgcagg
     3661 gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa ctgaccgatg
     3721 agtggccgca atatcccggt gacgagataa aacaaatcgt tgaacaaagc ggcatgtttc
     3781 ttggtgataa cgctgtaaga accctgacag gtttgataga gaaacagcat cagcaagcgc
     3841 aggtaatttc agccgatcat gtgctggggt tgctgcaaag ggtgccagac atcgcgtcat
     3901 tgaatattat tgatacgcag ttggttgaga atattaccgg gcatttatta cgttgccttg
     3961 ccgcaccggt gtggattgct gagcaccgcc aaagtagcat gaataacctg aaagcagcct
     4021 ggcctgcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa cagctcgata
     4081 ttcccctttt cgacagcgat ctgatcggtt tgtattttgc ctgtgcgctg gagcggcatc
     4141 aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc actattaatc
     4201 agctcgccat tgagcgcgat gttttaaatt gtcgggtaat tattgcccgt agcttaagcg
     4261 aacttgttgc cattcgcgaa gaggttgagc cgtt
//
LOCUS       DAJTYD010000227         4195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTYD010000227 DAJTYD010000000
VERSION     DAJTYD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.231"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OO585_003720"
     CDS             complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OO585_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6631157.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            1291..1827
                     /gene="sutR"
                     /locus_tag="OO585_003721"
     CDS             1291..1827
                     /gene="sutR"
                     /locus_tag="OO585_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6631158.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1900..3861
                     /gene="rlhA"
                     /locus_tag="OO585_003722"
     CDS             1900..3861
                     /gene="rlhA"
                     /locus_tag="OO585_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6631159.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3953..4183)
                     /locus_tag="OO585_003723"
     CDS             complement(3953..4183)
                     /locus_tag="OO585_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR6631160.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1093 a         1092 c         1054 g          956 t
ORIGIN      
        1 cgtctttcag ggcgtgtagg atgtcaatat tggtttctgt cagcgattaa attcaacacc
       61 acgtaacaaa cgcctccggc aattaatccc cagaacgcag aaccaatccc gaccagcgtc
      121 aatccacttg ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc
      181 agcgcctgat acaaactgcc gccaatggta ctcaacagcg ccagacctgc cagcatctgg
      241 atccaactta cgggcagggc agccatcatc tcggtaatgg cactaccaaa cagacctgcg
      301 agcaaataga aaatgcctgc aacggcagct gccagccaac gttgatcttt atccggatgc
      361 gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga ataaacgccg
      421 aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga aacaggagcc
      481 gaatatccag ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc caccgtcacc
      541 agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa
      601 gtggggagaa cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg cgcgatgacg
      661 atcacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac ggccttggtt
      721 gccagccata ccagcaacat acttccacac aacgtaaatt gaccgtccag actggcaaac
      781 gcctgtaaac caaagcgtaa taaaatctcg gcaagcattg ccgccgcaag cgagtgcgga
      841 ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat taacgcgttg
      901 gtgacaataa aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac
      961 aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca taatgtcaga
     1021 gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga gatttgtgca
     1081 gtggtggctc tggcgacaat cgctgcttgc cagattattg ccgctgaact ggcgtagcca
     1141 attaataccg ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa cagacgcata
     1201 gtcacctcgt acgttatagc gtacaggcac cgtagcattt gtccgttata acgcacaagt
     1261 gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt tttatccacc
     1321 acacttaaac aactacgcca gcagcgcggc tggagtcttt cgcgactggc agaagcgacg
     1381 ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg agtccagtcc gacggtcgcg
     1441 acattatgga aaattgccac cgggttgaac gtaccatttt caacatttat ttctccgccg
     1501 cagtccgcta cgccttcagt ttatgatcca caacagcagg cgatggtgat cacttcgctg
     1561 tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc atctggtgca
     1621 ataagcgaat cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga
     1681 caacttgatc tgtgcgttga tggggaatgg cagaccctta attgtgggga aggcgttcga
     1741 tttgctgcag acgtcacgca tatctatcgt aatggtgggg agcaaaccgt acattttcat
     1801 tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg
     1861 gctaaaatag ccgccatttt tcagctactg gataagaatg tgaccgtatc ttctcatcga
     1921 cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat tttgcacggt
     1981 gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc cagtaatagc
     2041 ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc
     2101 acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct gattactgac
     2161 ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct ggaacttgat
     2221 attccgccga ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt tgaaaaagcg
     2281 aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagagct gaatcttgat
     2341 cagatccgcg cgattcacca ggctacggac gcgaccattg aattctttat tcatggggca
     2401 ctgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc
     2461 aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga cgatcagggg
     2521 cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca gactgccaac
     2581 ctcggcgcgc tgattgatgc tggtgtacgc tccttcaaga ttgaagggcg ttacaaagat
     2641 atgaactacg tgaagaatat caccgcccat tatcgccaga tgcttgatgc cattattgaa
     2701 gaacgtggcg atctggcgcg cgcttcatca ggtcgtactg aacatttctt tgttccatcg
     2761 acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg taaaggcgat
     2821 attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt attgaaagtg
     2881 gcgaaagatc atctcgatgt tgccgtcacc gagccactgg caaatggtga tggcctgaac
     2941 gtgatgatta aacgtgaagt catcggtttt cgtgccaata cggtcgagaa aaccggagaa
     3001 aatcagtacc gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat tcgtccacat
     3061 cacccactaa accgtaatct tgatcataac tggcagcagg cactgacaaa aacctccagc
     3121 gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact gattctgacc
     3181 ctcaccagtg aagagggtgt cagcatcacg catacgctgg acgggcagtt cgacgaagcc
     3241 aataacgccg aaaaagcaat gaacaatctg aaggatggtc tggcaaaact ggggcaaacc
     3301 ctctattacg cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt accaaacagt
     3361 ctgttaaacc agttccgccg tgaagctgct gacatgctgg atgctgcgcg tcttgccagt
     3421 taccagcgcg gcagccgtaa accggttgct gagcctgcgc cggtttatcc gcaaacgcat
     3481 ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca tcgctatggt
     3541 gtgcagctga ttgacgcggc gtatgaggca catgaagaga agggcgaagt cccggtgatg
     3601 atcaccaagc attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat
     3661 atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt attaacgcta
     3721 aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa tcacatactg
     3781 aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgagct gctgaaaaca
     3841 ttgcctaagc gaaaagggta aaacgccagt tttctggtta ctcacaactc attgaatctg
     3901 catgatattg cctgccgggt aaggcgtcac gccgcatccg gcatcaaacg actcaacgtt
     3961 gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg
     4021 actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc tgatgatcca
     4081 cattctttac ccacacaaat tcatgtcctt tgtgccctgc catgagttgt cctgagaaca
     4141 gcgcacacgt taataagaca accgataacg ccttcgtaaa catcctgcca ccttt
//
LOCUS       DAJTYD010000228         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTYD010000228 DAJTYD010000000
VERSION     DAJTYD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.232"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO585_003724"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO585_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6631161.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO585_003725"
     tRNA            complement(324..399)
                     /locus_tag="OO585_003725"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO585_003726"
     tRNA            complement(406..480)
                     /locus_tag="OO585_003726"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO585_003727"
     tRNA            complement(597..681)
                     /locus_tag="OO585_003727"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO585_003728"
     tRNA            complement(690..765)
                     /locus_tag="OO585_003728"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO585_003729"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO585_003729"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6631162.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO585_003730"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO585_003730"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6631163.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO585_003731"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO585_003731"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6631164.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNVVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1040 c          932 g         1132 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gtaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc acattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJTYD010000229         4171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTYD010000229 DAJTYD010000000
VERSION     DAJTYD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.233"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1190)
                     /locus_tag="OO585_003732"
     CDS             complement(<1..1190)
                     /locus_tag="OO585_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6631165.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATEHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAA"
     gene            1235..1564
                     /locus_tag="OO585_003733"
     CDS             1235..1564
                     /locus_tag="OO585_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6631166.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            1551..1943
                     /gene="tnpB"
                     /locus_tag="OO585_003734"
     CDS             1551..1943
                     /gene="tnpB"
                     /locus_tag="OO585_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6631167.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            1950..3551
                     /locus_tag="OO585_003735"
     CDS             1950..3551
                     /locus_tag="OO585_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCR6631168.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(3686..3946)
                     /locus_tag="OO585_003736"
     CDS             complement(3686..3946)
                     /locus_tag="OO585_003736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631169.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(3994..>4171)
                     /locus_tag="OO585_003737"
     CDS             complement(3994..>4171)
                     /locus_tag="OO585_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCR6631170.1"
                     /translation="GDERGDIRNAQIVKAVFGAQGMNVALKDVMLCWGEDEDKPEVDP
                     FAALEDALSFAAQS"
BASE COUNT          973 a         1221 c         1076 g          901 t
ORIGIN      
        1 ctggcagcaa tatttctgtt caggcgttcc tgtgtggccg ccagacgggc ctctgcagca
       61 atctgcatct ccgtcccgcg ggctgccgcc acggcctgct gagcacggta cacggctgcc
      121 cttgcccgcg ccgtggcaat ctgcgttccc ctgaactgtg cttccgccag tgcaacttca
      181 ttacgtgcag ccgtcacaag tcctgccgtg gcagacatcg ctccggaggc catattgcca
      241 aagtaccggg caaccccgac ggcaaccagc gcccccacag ctgttgccac attatcaatc
      301 tgtccggcaa caccgttcag cacgccggag agcgtttttg tcaccccgca ggcctcattc
      361 acttcgccca cccaggccat aaaggcgttt tccaccttcg tgatactacc ggaaaccgtt
      421 tccggcatgg ccgcatattc atcacgtaat atccccagct ggctgattaa cgcagggacc
      481 actttatccg ctgtcagttt tccgtcatcc gccattgcct tcagatcttt acgggccaca
      541 cccatccccg cagcaagcgc gcggatcacc cggtcgccat tttcgttaac agcattaaat
      601 tcttcgccac gcagtacacc ctgtgccagt gcctggctga actgggtgat caccgaaccg
      661 gattccgcaa ctccagcccc tgacagtttc agtcctgtcg aaatggcctc cgtcaccttc
      721 agcacatcat cagcactgta accatattca cgcatcgagg cagccgaacg ggcaaacagg
      781 gccgcattat ccgaaaacgc ggtacctgtt cgctgactga tatccatcag cactttctgt
      841 gatgacgaga attcatccga tgactgagat gcctgtttca gacgggcatt cacggaaccc
      901 cactcatcgg ccagcgaaat caggtgttcg gtggcaaagg caccggcaaa tgcgccagcc
      961 attccgacag cagaaccgcg aatttccgtc aactgactgt tcagttctgc aagggcgcgg
     1021 cgctgttccc gtgcggcagc agcggcatga cgtccgccat tctgcagggt ccggtaatat
     1081 tcactgccca tacgggacgc ccgctggatc tccgactgga atgactgtga atttgccgaa
     1141 attttgagtg tcgttacggg gaagtcatct tcacagggat cttcggtcag ttagtattac
     1201 cgacatccat tcaccaactt accggagata atcgatgtca aaaccccgat ggaccattga
     1261 acagaaaagg cagcacgttg ctgcctggcg cgccagtggt cttacacgtc agcaatactg
     1321 tgaactcaac gatattccct tcacatcact tcgcgaatgg ccgcaggatg tcgcaaaagc
     1381 tgaacgccgg gcaaatgaac cgaccgttct tcccgttcat attgcgccac cactgcatgc
     1441 tgatgtacca cagcccgtca cgaatgagcc agtcacgctc ttccttcccg gtggagtacg
     1501 gatgtgctgt cagccgtcac aacttaccga tgtcttcagg gcactcaaat atgctcagtc
     1561 ctgataatgt cttcatcgca attaaacctg ttgacatgcg ccggggcatc gactcactga
     1621 cgcagtatat acaggatgaa ctccggtcga catggcatga gggagccgcc ttcgtctttg
     1681 ttaacaaagt ccgttcgcgt atcaaagttc tccggtggga taaacacggg gtgtggttgt
     1741 gtacccgccg tctgcacaaa ggcagcttcc gctggccacg tgcaaatgac gctgcctggc
     1801 acctcactcc cgacgagttt aactggctga ttgccggtgt tgactggcag caggttaagg
     1861 gacatgacct gacgaaatgg gtctggcaga atgaacctga actgcgccct gaaaacacgc
     1921 aaaatacact gctaactcag tgaaaaataa tgaatatccg tatctggagt ggtatactcc
     1981 cctgtatgga tatctccgct ctcaacacca cgaatgacat cgaaaaactg cgtgctatgg
     2041 cacttgccat ggtacaagaa gtcatgtcgg agaatgccga aaaagagcgg gaattactgg
     2101 agaaaagccg gcgcatccag cttctggaag aaatgctgaa actggttcgt caacagcgct
     2161 tcggaaaaaa atgtgaaacg ctggctggta tgcaacgctc cctattcgaa gaggatgttg
     2221 atgccgatat cgccgcgctt accgcacatc tggataaact gctcccgcaa tcccctgaag
     2281 aagatgaaaa agcgtcccgt tcacgcccga tacgcaaacc cttaccggtt catcttccac
     2341 gggtggaaaa aatgatccag ccggacactg accattgccc tgaatgtgac gagccgctgc
     2401 actatatccg cgatgcggtg agtgaaaagc tggagtatat tcccgctcac tttgtggtga
     2461 accgttatgt ccgtccgcaa tacagttgtc cctgttgcca gaaggtgttc agcggtgaaa
     2521 tgccggcaca tatcctcccg aaaagtgccg ttgagccatc agtcatcgca caggtgatca
     2581 tcaataaata cggtgaccac ctgcctctgt atcgccagca acaggtcttt gcccgttcag
     2641 atgtcgggct gcccgtcagt tcgatggctg acatggttgg cgcggcgggt gccgcattat
     2701 ctcccctggc ggcgttactc catcgcgagt tgataaaccg tccggtggtg catgcagatg
     2761 agactaccct gaagatcctg aacacgaaga aaggcggtaa atcctgctcc ggttatctgt
     2821 gggcatacgt cagtggagaa aggacgggac cgtcagttgt gtgcttcgac tgccggaccg
     2881 gacgtagcca tgagtatcct gaaaactggc ttcagggctg gggcgggacg ctggttgtcg
     2941 acggacataa agcttaccgg actctggcaa acaaagtgcc ggagatcacg ctggccggat
     3001 gctgggccca tgcccgcagg ggcttcgccg acctgtataa aatcagtaaa gatccacggg
     3061 ctgccatagc cgtgaagaaa atcgcggggt tgtaccgtct tgagaagaag atcagtagcc
     3121 gccccgtgga aaaaatccgc cagtggcgac agcgttatgc ccgtccgata ctggaagaac
     3181 tgtggtcatg gcttgaagag caggaaccgc aatgttctcc gggaaaggca ttacacaaag
     3241 ctattgccta tgcgctgtct catcgcgtgg aactgagccg cttcctggaa gatggtgcgg
     3301 tgccgctgga taataatgtg tgtgaacggg ccatcaaaaa cgtggttctg ggcagaaaat
     3361 cgtggctgtt cgccggttcg cagatggcgg gagaacgcgc cgcgcaaata atgagcttgc
     3421 tggaaaccgc gaaacgcaac ggtctggagc cgcatgcctg gttgacagac gtcctgatgc
     3481 gtctgccgga gtggccggag gagcgactgg cagagttgct gcctcttgag ggatttacct
     3541 tctccgggtg agtgatacct gccgtcaggt gttcgtgcac cgggccataa cctgcagtcg
     3601 ggagttgaac tcctgactgc aggaaatgag ccagaccagc aaccacgcgg gctgacagcg
     3661 aataagaacc tgaatcagcc cggagtcatt cccgggtcag tacatcatca cctgcaatta
     3721 atccccgagt cagtacttcg cggcctgacg cgatcacgct actcagtact tcagagcccg
     3781 cctccggtac ctgcctggcg atcgcctctg tgcaacaaaa taaatcaacc gttatcaaac
     3841 cacgagcgtt cgtatgcgcc gccgatgacg cgcgcctgaa aaacgcgtca atatgagttc
     3901 cccggaagct tacaattttg ataatcagtt cacgtaacgt cgccattcac ctttctccgg
     3961 gcgtaaaaaa accgcctcag cggttctcat cattcatgac tgtgctgcaa agctcagcgc
     4021 gtcttccagc gccgcaaacg gatccacctc cggcttatcc tcatcctcgc cccagcagag
     4081 catgacgtcc ttcagtgcaa cattcatccc ctgtgccccg aaaaccgctt tcacgatctg
     4141 tgcattacgg atatccccgc gctcatcacc c
//
LOCUS       DAJTYD010000230         4068 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTYD010000230 DAJTYD010000000
VERSION     DAJTYD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.234"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1046)
                     /locus_tag="OO585_003738"
     CDS             complement(<1..1046)
                     /locus_tag="OO585_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6631171.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OO585_003739"
     CDS             1537..1647
                     /locus_tag="OO585_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6631172.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OO585_003740"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OO585_003740"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6631173.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="OO585_003741"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="OO585_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1089 a          829 c          953 g         1197 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcatctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat atcgcaat
//
LOCUS       DAJTYD010000231         3969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTYD010000231 DAJTYD010000000
VERSION     DAJTYD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.235"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..924
                     /locus_tag="OO585_003742"
     CDS             <1..924
                     /locus_tag="OO585_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6631174.1"
                     /translation="RVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRF
                     MPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGL
                     GIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLL
                     ECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYV
                     GIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKE
                     SRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(909..1646)
                     /gene="trmN"
                     /locus_tag="OO585_003743"
     CDS             complement(909..1646)
                     /gene="trmN"
                     /locus_tag="OO585_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6631175.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1778..3112
                     /gene="srmB"
                     /locus_tag="OO585_003744"
     CDS             1778..3112
                     /gene="srmB"
                     /locus_tag="OO585_003744"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6631176.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3321..>3969)
                     /locus_tag="OO585_003745"
     CDS             complement(3321..>3969)
                     /locus_tag="OO585_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6631177.1"
                     /translation="REAAKKGSDPDGELRVVSGETLLSYRMPQVLQRFRQRAPKVRLS
                     LQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELGEQSLVLVASPQIADVDFTEP
                     GRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIELLSIESIKRCVAANIGVSYLP
                     RFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVSPAMHTFIQCIEECFLPG"
BASE COUNT          994 a         1100 c         1014 g          861 t
ORIGIN      
        1 cgggttgcca atctcgaatt taatcagttc caccctaccg cactgtatca cccacaggca
       61 cgcaatttcc tgttaacgga agcactgcgc ggcgaaggcg cttatctcaa gcgcccggat
      121 ggcacgcgtt ttatgcccga ttttgatgag cgcggcgaac tggccccgcg cgatattgtc
      181 gcccgcgcca ttgaccatga aatgaaacgc ctcggcgcag attgtatgtt cctcgacatc
      241 agccataagc ccgccgattt tattcgccag catttcccga tgatttatga aaaattgctc
      301 gggctgggga ttgatctcac acaagaaccg gtaccgattg tgcctgctgc acactatacc
      361 tgcggtggtg taatggttga tgatcatggg cgtacggacg tcgagggctt gtatgccatt
      421 ggcgaggtga gttataccgg cttacacggc gctaaccgca tggcctcgaa ttcattgctg
      481 gagtgtctgg tctatggctg gtcggcggca gaagatatca ccagacgtat gcctgatgcc
      541 cacggcgtca gtacgttacc accgtgggat gaaagccgcg ttgagaaccc tgacgaacgg
      601 gtagtaattc agcataactg gcacgagcta cggctgttta tgtgggatta cgtcggcatt
      661 gtgcgcacaa caaaacgtct tgagcgcgcc ctgcggcgta tcaatatgtt gcaacaggaa
      721 atagacgaat attacgccca cttccgcgtg tcgaataatt tgctggagct gcgtaatctg
      781 gtgcaggttg cggaattgat tgttcgctgt gccatgatgc gcaaggagag tcgtggcctg
      841 catttcacac tggattatcc ggaactgctc acccattccg gtccgtcgat cctttccccc
      901 ggcaatcatt acataaacag ataaaaagcc tgggtcagcg ccgtatacgc ttcggaatag
      961 ttctggtctg gcccacgaat gactaagcga tcgctaaagc attctcccgc ctgcggggag
     1021 aatgccagca gcacccgatg cggcagtcgc gcttcgtttt ccgccacatc cgtccgcaaa
     1081 cgtaaatgcc agcccatgct taatgccagc tccgtaaaac cattaccaat ctgctctggc
     1141 agcactacgc agaaaaatcc ctcttcggta atgcactccg ccgcacaggt cagcagcgat
     1201 gggtgatcaa gcgtagtggt atagcgagcc tgttcccgtt gagggatcgc gcactctact
     1261 ccctgctgat agtaaggtgg gttactgatg attaaatcga agcgtactgt ctgctgtgtg
     1321 agccactgct gaatatccgc cgtatggacg ttaatccgct ctgcccacgg ggactggttg
     1381 atattttcct gcgcctgcgc ggcagcttca ctttccagct caacagcatc aattatcacg
     1441 ctgtcactgg ttcgctgcgc cagcatcagt gccagcaacc cgctacccgc gccgatatca
     1501 aggcaacgtt tcaccccagc caccggtgcc catgcgccca gcaaaatgcc atccgttccc
     1561 actttcatcg cacagcgatc gtgagcaaca aaaaactgtt taaaagtaaa tccattacga
     1621 cgaagcacgg atgtagactg tgacatgaaa ataaaacctt gcaggaaaaa cggcgatagc
     1681 accgggtgag aacaataccc gagaagcgat atccatacaa acagatgaag attgcagccg
     1741 taacgtctat aatcagcgcc ccacacagag gtagaacatg actgtaacga ctttttccga
     1801 acttgaactc gacgaaagcc tgctggaagc cctccaggat aaaggtttca ctcgcccgac
     1861 cgccattcag gctgccgcca ttccgcctgc gctcgatggc cgtgatgtac tcggttctgc
     1921 gccgacaggc accggtaaaa cggcggcgta tctgctgcca gcgttgcagc acctgctcga
     1981 tttcccgcgt aagaaatccg gtccgccgcg tattttgatc ctcaccccaa ctcgcgagct
     2041 ggcgatgcag gtggccgatc atgcccgcga actggcgaaa catacgcatc tggatatcgc
     2101 caccattacc ggcggcgtag cctatatgaa ccacgcggaa gtgttcagcg aaaatcagga
     2161 catcgtggtc gccacgaccg gacgtctgct gcaatacata aaagaagaga acttcgattg
     2221 ccgcgcggtt gaaacgctga tcctcgacga agcagaccgt atgctggata tgggcttcgc
     2281 tcaggatatc gaacatattg ctggcgaaac gcgctggcgt aaacagaccc tgctcttttc
     2341 ggcaacgctg gaaggcgatg cgattcagga ctttgccgag cgtctgctgg aagatccggt
     2401 ggaggtttct gccaatccgt ccacccgtga gcgcaaaaaa attcatcagt ggtattaccg
     2461 cgccgatgac ctggaacata aaaccgcatt gctggtgcat ctgttaaaac agccggaagc
     2521 gacccgctca attgtgtttg tgcgtaagcg tgaacgtgtg catgagctgg caaactggct
     2581 gcgcgaagcg ggcatcaaca actgctatct cgaaggtgag atggtacagg gcaagcgtaa
     2641 tgaagcgatt aaacgcctga ccgaaggccg cgtaaacgta ctggtagcaa ccgatgttgc
     2701 cgcgcgcggt atcgacattc cagacgtcag ccacgtcttt aacttcgata tgccgcgcag
     2761 tggtgatact tatttgcacc gtatcggacg taccgcgcgc gccggtcgta aaggcaccgc
     2821 gatttcgctg gtggaagccc atgaccatct gctgcttggc aaagttggcc gttatattga
     2881 agagccgata aaagctcgcg tcattgagga actacgcccg aaaacgcgta cgccaagcga
     2941 gaagcagacc ggcaagccat cgaagaaagt actggcaaaa cgcgccgaga agaaaaaagc
     3001 taaagagaaa gagaagccgc gggtgaaaaa acgccatcgc gacaccaaaa atattggtaa
     3061 gcgccgtaaa ccgagcggaa cgggcgtgcc accgcaaacg acagaagagt aatcttaatt
     3121 ccgggtttaa gcccggaatt aaaagtgctg gcaaacgcaa aactgcctga tgcgctacgc
     3181 ttatcaggcc tacatggctc atgcaatata ttgaatttgc acgatcttgt aggccggata
     3241 aggcgttcac gccgcatccg gcatgaacaa aacgcacttc gtcgacaagt tgggtgccgg
     3301 gtttaagccc ggcattattt ttatcccggc aaaaaacact cttcaataca ctgaataaaa
     3361 gtgtgcatcg ccgggctaac cgcttttcca gcatggtgtg cgcacatagc cgtaatggtc
     3421 tgcgactgtt cgccaaaagg caattcaatt aattctccgc tctcaagctc tttttccacc
     3481 gcaaagcggg gcaagtaact gacaccgata ttcgccgcga cacaacgttt gatactttca
     3541 atgcttaaca gctcaatggt gttttccacc gtgatccgcc gctgacgcag cgtgctctca
     3601 aatatctgcc ggaagacaca ttgcggttcg ttgataataa agctacaggc gttatgtctt
     3661 cccggctcag taaaatcgac atctgcaatt tgcggtgaag ccaccagcac cagcgattgt
     3721 tcacccaact ctcgtcggtt cagggcatca tcattcccta cgcgataaaa gacgccgaca
     3781 tcggcctcat cattcagtag cgcatcacgg atcacgtaac agttcagcga ctgcaacgat
     3841 aaacgcactt ttggtgcgcg ttgccgaaaa cgctgcaaca cctgcggcat tcggtaagag
     3901 agcaacgttt cgcccgaaac aacgcgtagt tccccgtccg gatctgaccc ctttttggca
     3961 gcctcacga
//
LOCUS       DAJTYD010000232         3934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTYD010000232 DAJTYD010000000
VERSION     DAJTYD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.236"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..354
                     /locus_tag="OO585_003746"
     CDS             <1..354
                     /locus_tag="OO585_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR6631178.1"
                     /translation="NASELTRWAGRKCPSGRVMGLANKGWIKGTPQDGGWIGWMIKPL
                     GRWSLVMEIDEGFAVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVIDA
                     ITASELINDIEALFE"
     gene            364..606
                     /locus_tag="OO585_003747"
     CDS             364..606
                     /locus_tag="OO585_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6631179.1"
                     /translation="MDKELPWLADNAQLELKYKKSKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKRLSGVMKIFHWSGSPPSRRPST"
     gene            2130..3934
                     /locus_tag="OO585_003748"
                     /pseudo
     CDS             2130..3934
                     /locus_tag="OO585_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543415.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1021 a          998 c         1016 g          899 t
ORIGIN      
        1 aacgccagcg aactgacccg ctgggcaggc agaaaatgcc cgagcggtcg ggtcatgggg
       61 ctggcgaata aaggctggat aaagggcacc ccgcaggatg gaggctggat cggctggatg
      121 atcaaacccc tgggccgctg gtcgttagtc atggaaatcg atgaaggttt tgcggtaggc
      181 atgtcgccag ccgaactcag cgctgagcag ctcttaagca agctgtggct atgggaaggc
      241 aaagcagaaa gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtaatc
      301 gatgccatca ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag
      361 agcatggaca aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa
      421 aaaagcaaaa cgccgctcag tcatcgcaat tggccgggcg aaccggtgtc tgttatcact
      481 gaaagcatca ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaga
      541 ttgtctggcg ttatgaaaat ttttcactgg agtggcagtc cgccatcacg caggccatca
      601 acttgatcgg cgaacacaaa ccctcagtcc cggcccggac aatggcggcg ctagcctgta
      661 tcgcgcaaaa tgacagccaa cagttgctcg acgaaatcgt ccaacaagag gggctggaat
      721 atgcgactga ggtggtaatt gcacgcctgt ttattgcgcg gtgttatgag agtgatcctc
      781 tggtagtgac attgcagtat caggacgagg attatggcta tggttatcgc tcagaaacct
      841 ataacgaatt cgatctccga ctgcgtaagc atctctctct ggcagaggaa agctgctggc
      901 agcgttgcgc cgacaaactc attgccgcac taccaggaat aaccaaagtt cgccgccctt
      961 ttattgcgct gatcctcccg gaaaaaccag aaatagccaa tgagttggta ggccttgaat
     1021 gcccgcgaac tcattttcat tctaagaagt ggttaaaagt tgttgctaat gaccccacag
     1081 cggtgagaaa actcgaacac tactggagcc aggatatatt tagcgatcga gaagccagct
     1141 acatgtcgca tgaaaaccac ttcggctacg cggcctgcgc cgcccttttg cgcgaacaag
     1201 gactggcagc cattccgcgc ctcgcgatgt atgcccataa agaagattgc ggcagtctgc
     1261 tggtacaaat taaccatccg caagtcatcc gcaccttgct actggtggct gataaaaaca
     1321 aacccagcct gcaacgtgta gctaaatacc ataaaaactt cccccatgcg acgctcgccg
     1381 cactggcaga actgctggcg ttaacagaac caccagcccg ccctggttat ccaatcatcg
     1441 aagacaaaaa gctgcctgca cagcaaaaag cacgcgatga atactggcgt acgctgttac
     1501 aaacgctgat ggcatcgcag ccacaactgg cagaagaggt gatgcagtgg ttaagtactc
     1561 aagccagggc agtgctgaat agttatttat cggcaccgcc caaaccggtt attgatagta
     1621 ccgataacag caatctgcct gaaatcctcg tttcgctacc gtggcgtagt aagaaaaaaa
     1681 tgacagctcc acgtcttgat ttggcaccgc tcgaattaac tccgcaagtt tactggcaac
     1741 caggcgaaca agagaggctt gccgccactg agtctgcccg ttatttcagc acggaatctc
     1801 ttgcgcaacg catggaacaa aaaagtggac gagttgtatt acaggaactg ggttttgggg
     1861 atgatgtatg gctgtttctg aattatatac tccccggaaa actggatgct gcacgcaatt
     1921 cactcattgt tcagtggcat tactaccagg ggcgggttga agagatcctg aatggctgga
     1981 actccccgga agcacaatta gcagaacagg cgctccgcag cggtcacata gaagcgttaa
     2041 ttaacatatg ggaaaatgac aactactcac gttatcgtcc ggaaaagagt gtctggaacc
     2101 tgtatttatt ggcacagttg ccgcgtgaga tggctttgac cttctggctg cgtatcaatg
     2161 agaaaaagca tctgttcgcg ggtgaggact attttctcag tatcctcgga ttggatgcgc
     2221 taccaggtct gctgttggct ttttcacatc gtccaaaaga aacatttccg ttaattttaa
     2281 attttggcgc aacagaactg gcgctgcctg ttgcccgcgt ctggcgacgt ttcgcggcgc
     2341 agcgtgatct ggctcgccag tggattttac aatggccgga acatacggct agtgcactta
     2401 tccctcttgt ctttaccaaa cccagcgata atagcgaagc cgcattactt gccctgcgtt
     2461 tactgtacga acagggacat ggcgaattgc tacaaaccgt ggcaaaccgc tggcagcgta
     2521 cagatgtatg gtctgccctg gagcagttgc ttaaacaggg tccaatggac atttacccgg
     2581 cacgcattcc aaaagcccct gatttctggc atccggcaat gtggtccagg cctcgactta
     2641 tcactaataa tcagcctgtt accggtgacg ctctggaaat tatcggcgaa atgctgcgct
     2701 ttacctaggg gggacgtttt tatagcgggc tggaacaact gaaaacgttc tgccagccac
     2761 aaacgctggc cgcttttgcc tgggatctct tcactgcctg gcaacaagcg ggggcccccg
     2821 caaaagacaa ctgggcattt ctggcattac gtctctttgg tgacgaaagc acggcacggg
     2881 atctgacgac acagatcctc gcctggccac aagaaggcaa atctgcccgt gctgtcagtg
     2941 gtctgaatat acttaccctg atgaataatg atatggcgct gatgcagctg catcatgtat
     3001 cacaacgggc gaaatcacgc cccttacgtg aaaacgcagc ggaatttctt caggtggtcg
     3061 cagaaaatcg cgggctaagc caggaagagt tagcggacag attagtccca accctgggcc
     3121 ttgatgatcc gcaggcgttg agttttgatt ttggtccccg gcagtttacc gttcgcttcg
     3181 atgaaaacct caacccggtt atctttgatc agcaaaacgt tcgccagaaa agcgttcccc
     3241 ggttgcgcgc cgatgacgat caactgaaag cgcccgaggc actggcccga ctaaaagggc
     3301 taaaaaaaga tgctactcag gtgagcaaaa acctgctccc gcgtcttgaa gctgccctac
     3361 gtaccacccg acgctggtcg ctggcagatt ttcattctct gtttgttaat catcccttta
     3421 cccgtctggt tacccagcga ttaatatggg gggtttatcc ggcaaatgaa ccgcgttgtt
     3481 tactcaaagc ctttcgtgtg gccgcagagg gggagttctg caatgcgcaa gatgagccaa
     3541 ttgacctgcc tgcggacgct ctgattggca ttgcccaccc gttagaaatg acagtagaaa
     3601 tgcgcagtga atttgcacag ctttttgccg attacgaaat tatgccgcct tttcgccaat
     3661 tgtcgcgccg cacggtgctg ctcacacctg acgagtcaac cagtaacagc ctgactcgct
     3721 gggaaggtaa atccgctacc gttgggcagc ttatgggaat gcgatacaaa ggctgggagt
     3781 caggttatga ggacgcattt gtctatgacc tgggtgagta ccggctggtc cttaagtttt
     3841 cacccggttt taaccactac aatgttgata gcaaagcgct aatgagcttc cgttctcttc
     3901 gagtgtaccg tgacaataaa tccgtcactt ttgc
//
LOCUS       DAJTYD010000233         3837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTYD010000233 DAJTYD010000000
VERSION     DAJTYD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.237"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(521..712)
                     /locus_tag="OO585_003749"
     CDS             complement(521..712)
                     /locus_tag="OO585_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6631180.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            780..1103
                     /locus_tag="OO585_003750"
     CDS             780..1103
                     /locus_tag="OO585_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6631181.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            1066..1809
                     /locus_tag="OO585_003751"
                     /pseudo
     CDS             1066..1809
                     /locus_tag="OO585_003751"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1781..2341
                     /locus_tag="OO585_003752"
     CDS             1781..2341
                     /locus_tag="OO585_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6631182.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            2348..2581
                     /locus_tag="OO585_003753"
     CDS             2348..2581
                     /locus_tag="OO585_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6631183.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
     gene            complement(3051..3518)
                     /locus_tag="OO585_003754"
     CDS             complement(3051..3518)
                     /locus_tag="OO585_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631184.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          912 a          877 c         1146 g          902 t
ORIGIN      
        1 accaggggga aacgcctggt atctttaagt cctgtcgggt ttcgccacct ctgacttgag
       61 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc caccggcgcg
      121 gcctttttcg ggctcttgtt ttttgctcac atgtttcttt tggctttatc ccctgattct
      181 gtggataact gtgttaccgt ctttgagtgc gctgataccg ctcgccgcag ccgaacgacc
      241 gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc ggaagctaca ttttctcctt
      301 acgcatctgt gcggcatttc acacccggca tggtgcactt ttcatacaat ccgcactgat
      361 gccgcataag taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
      421 cccgacaccc gctaaaacct gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
      481 ctcacaggcc agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccatcat
      541 ccccgaagcg tgcgaggcag ctgcggtaaa gctcgtcagc gtggtcgtga agcgcttcgc
      601 agatgtcagc ctggtcgtcg gcgtccagtt cattcagttt ttccagcagg gtcagcgtct
      661 ggcttctgat gaagcgggcc atgttaaggg cggttttttc ctgtttagtc acagttctgt
      721 ttctccatgt gtggggagtc tgtcggaggt ttttaatgat accggtgaaa cgcgaaagga
      781 tgctcacgat acgggtcacg gatgaggaac atgcgcggct gctggcacgt tgtgagggaa
      841 aacaacttgc gtcatggatg cgtaaggtct gcctgggggc accgccatcg aaaacgtcag
      901 gtcttccgac actggcaccg ccgctgttgc gccagttcgc ttcggtcggc aataacctga
      961 atcagatagc ccggaagata aacagcggac agtggtcggg gcatgaccgg gttcatgtgg
     1021 tggctgcact gatggccatc ggacgtgaac tgagtgagct gagggatgag gtccgtaaac
     1081 agggagagcg ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg
     1141 gttgattacc tgctgggccg ggagcgtaac cgggaagggg cgagagttct gcggggtgct
     1201 cccgaagagg tgcgggaact catcgatgcc acgccgtttg cgaaaaaata cacctcgggg
     1261 gttctgtctt ttgcagagca gaccctgcca ccgggagagc gtgaaagggt gatggagagc
     1321 tttgagcggg tactgatgcc cggtcttgaa aaaaatcagt acagcatcct gtgggtggag
     1381 caccaggaca aggggcgtct tgagctgaat tttgtcatcc cgaatatgga gctggcgagc
     1441 ggaaaacggc tccagcccta ttacgaccga gcggaccgtc cgcgaatcaa tgcctggcag
     1501 acactggtga atcaccatta cgggctgcac gacccgaacg caccggagaa caggcggaca
     1561 cttgtcaccc cgaataatct gccgaaagcg aaacaggagg ccgcagaagc gattacgagg
     1621 ggactggagg ctctttacca tgccggagcg ctaaaaacgc gtcaggatgt cactgaggcg
     1681 cttacagcgg caggttttga ggttgtgagg acaaccagga gcagtatcag tatcgccgac
     1741 ccggagggcg ggcgtaatct caggctgaaa ggggcgctgt atgagcaatc ttttacaaat
     1801 ggggacagac ttcgagaaga aactgaaaga gcgtgccgtc tctacagaga acatactgaa
     1861 cagcgagttc aggaagctcg aagcgtctgt agacagggaa ctgaacttaa acgggcagaa
     1921 aatcagagac gctatcagcg cacacacagc agcggtgaaa gaacaactgg agacgctgaa
     1981 cagtgccgtg aacgcgcagt tcagcgccac agagaacgaa ctggcccggc ggcaggaaga
     2041 gctgctctgg aagctggtca aagggcggat actgtacccg tcgctgacgg cgctttgcgt
     2101 cacgggcggg atttttctgg cgtcatgggg gctgattcag tggcaggaaa gccggattgc
     2161 ggcgaacatc ctggctatca gggaccagga agagacgctg gcgaaactcc gggaaaaaac
     2221 atggggtgtg acgtatcacg agggcaggaa cgggaaattt ctggtattgc cgtccggggt
     2281 gaagggggaa aacaactgga cggtggagaa gaagaacgca gtaagactgg tcagggagta
     2341 agtgagtatg acagagctgg aaatgcactt actgaacgcc ttagagcaat tgcagcagga
     2401 ctatacgcag cggctgaaag actgggagag cgccttcacg gatttgcagc atatgttcga
     2461 gctgacacga caggagaacg ggcaactgag ggaacagtgc gacgacttga gtcagcaggt
     2521 gcagagatta gtcgagcagg tggaccactt gagtcggtta tttatcacga acagcagata
     2581 agggatgaac aggaaaggca ggcacgcctg gaacgggagc gtgcgcggtc ctggcatgaa
     2641 gagcaggaac gggaagcacg ggtatggcgt ggcccgtcaa tagggatatg acggtgagtt
     2701 gttccgggag gttgccagat ggtaccggaa cgcgttaaaa acgcatccgg gcgcgattct
     2761 ggcgtgtcgt ttttgttgct gtgttttgcc tctttgccag atcgcgctca ttcctcagcc
     2821 tcatcccgcg atgtctgcgt atgtcatcaa ctgaaaagat ggaaaaaaaa cagtgtcatg
     2881 aggtttacgc aggcaaaacc agcgttattc acatggctga atagctatgc atacggttaa
     2941 aatttatcag gtgcgatcgc ggcagctttt cgggtggttt gttgctattt ttacctgtca
     3001 gcagccgtga tcgcgctgaa cgcatttcag cggtgcgtac aattaagata ttacgttaaa
     3061 ttgtgaggga atggtcccca tatactgact ttatcatagg gatagggttg gtagtgggtc
     3121 gattgggttt cggcattgtt tccgttaatg ccaattgcat ttttgtattt gatgcaacag
     3181 aaggcgtgag attctcctgg gcctggaaag gctaagtgtt tagaattgat ggcaaaatat
     3241 cttccgtccg atttttgaag tagttgttgt ttggctgtct cccaaagggt gctgttacaa
     3301 acttcttgaa tttgtagttt ttgcagggct cttattactg cgttgtcatg ttccaattct
     3361 ttaggggagt gtattattct ctcttttaat aagctgttta ccacagggtt tagttgttgt
     3421 ctaaagcacg cagataatgc cattgctaca caattttgtt cccgaggaag catgtttggc
     3481 ggggtatgcc gtgtttgatg tgatatgtct attctcatag aaggtctctg gcttatagca
     3541 tcaacaaata cggctataaa acaaaatttt catagagtga ttcaagatct tcttgagatc
     3601 cttttttgcg gcgcgtaatc tgctgcctgc aaacaaaaaa accaccgcta ccaacggtgg
     3661 tttgtttgcc ggaatctaga gctaccaact ctttcttccg aaggtaactg gcttggtgga
     3721 gcacagatac caaatactgt ccttctagtg tagccgcagt taggccacca cttcaagaac
     3781 tcttaatatc tcaatccacc ttgtctagtt accaatggct gctgccagtg gcgttaa
//
LOCUS       DAJTYD010000234         3836 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTYD010000234 DAJTYD010000000
VERSION     DAJTYD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.238"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..214
                     /locus_tag="OO585_003755"
     CDS             <1..214
                     /locus_tag="OO585_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HCR6631185.1"
                     /translation="KGEAEAARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVR
                     ERPWCSVGTAAAVGIFIGALLSMRKS"
     gene            462..707
                     /gene="nrdH"
                     /locus_tag="OO585_003756"
     CDS             462..707
                     /gene="nrdH"
                     /locus_tag="OO585_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6631186.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            704..1114
                     /gene="nrdI"
                     /locus_tag="OO585_003757"
     CDS             704..1114
                     /gene="nrdI"
                     /locus_tag="OO585_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6631187.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1087..3231
                     /gene="nrdE"
                     /locus_tag="OO585_003758"
     CDS             1087..3231
                     /gene="nrdE"
                     /locus_tag="OO585_003758"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6631188.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENVQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3241..>3836
                     /locus_tag="OO585_003759"
     CDS             3241..>3836
                     /locus_tag="OO585_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6631189.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYY"
BASE COUNT          920 a          994 c          992 g          930 t
ORIGIN      
        1 caaaggggaa gctgaagccg cacgcagtaa agctcaggcg ttgctgaaag aaaccagggc
       61 gcgaatgcat ggtcgtactc gcgtccagca agccgcgcgc gatgccgttg gctgcgcgga
      121 ttcttttgtt cgtgaaagac cctggtgtag cgtgggtaca gcagctgcgg taggtatttt
      181 tatcggcgca ctgttaagca tgcgcaaatc gtagtgcaaa aatgataata aatacgcgtc
      241 tttgaccccg aagcctgtct tcggggtttc tttttgcctg gtgaatcaca aaaatccccc
      301 tcccccgtca cgctcatatc cagggtaatt tcgatcacta tttgctatat attgtgtagt
      361 tgaatctttt ttcaactaca tctagtatct ctgtatcaac agagagacaa cccgacgcgt
      421 atcatcgcgc cgtatcttca ttttaaacgg aaatacgaat catgcgcata actatttaca
      481 ctcgtaacga ttgcgttcag tgccacgcca ccaaacgggc gatggaaaac cgggggtttg
      541 attttgaaat gattaatgtc gatcgcgttc ctgaagcagc ggaagcgttg cgtgctcagg
      601 gctttcgtca gttgccggta gtgattgctg gcgatcttag ctggtctggt ttccgtccgg
      661 acatgattaa ccgtctgcat ccagcgccac acgcggccag tgcatgagcc agctcgtcta
      721 cttctccagc agctccgaaa acacgcagcg ttttatcgaa cgtttaggtc tgcccgcggt
      781 gcgcatcccg ctcaatgagc gggaacggat tcaggtagac gagccttaca tcctgatcgt
      841 gccctcttac ggcggcggcg gtacggctgg cgcggtgcca cgacaggtaa ttcgcttttt
      901 aaacgacgag cacaaccggg cgttgcttcg cggcgttatt gcttctggta atcgcaactt
      961 tggtgaggcg tatggccgcg ccggagatgt gattgcccgg aaatgcggcg tgccgtggct
     1021 gtaccgtttt gaactcatgg gtacgcaaag cgatatcgaa aacgttcgta aaggagtaac
     1081 cgaattttgg caacgacaac cgcagaatgc ctgacgcagg aaacgatgga ttaccacgcg
     1141 ctgaatgcga tgcttaacct ctacgatagc gcaggtcgca ttcagttcga taaagaccgc
     1201 caggccgttg acgcctttat ggcgacgcat gtgcgtccga acagtgtgac cttcagtagc
     1261 cagcagcagc gcctgaactg gctggtcaac gaaggttact atgatgaaag cgttcttaat
     1321 cgctactctc gcgactttgt cattacgctg tttgcctacg cacacaccag cggttttcgt
     1381 ttccagacat tcctcggggc atggaagttt tacaccagct atacgttgaa gacattcgac
     1441 ggtaaacgtt atctggaaga ttttgccgat cgagtcacga tggtggcgct gacgctggca
     1501 caaggcgatg cgacgctggc gttgcaactg accgatgaaa tgctgtcagg acgctttcag
     1561 ccagccacgc cgacattcct caactgcggt aagcagcagc gcggcgaact ggtttcctgt
     1621 tttctgctgc gtattgaaga caatatggag tcgattggcc gggcggtaaa ttccgcactg
     1681 caactgtcga aacgcggcgg cggcgtggca tttttgctgt cgaatctgcg agaagcgggc
     1741 gcgccaatta aacgtattga aaatcaatct tctggcgtaa ttccggtgat gaaaatgctg
     1801 gaagacgcat tttcctatgc caaccaactc ggcgcgcgtc agggggcagg tgcagtctat
     1861 ttacatgctc atcatcccga tattctgcgt tttctcgata caaaacggga aaatgccgac
     1921 gaaaaaatcc gcattaaaac actgtcgctg ggcgtggtga tcccggatat cactttccat
     1981 ctggcaaaag agaatgtgca gatggcgctg ttttcgccct atgacgttga gcgagtttat
     2041 ggcaagccgt ttgccgatat cgccatcagc gaacactatg acgaactggt tgccgatgaa
     2101 cgcatccgca aaaaatacct caacgcccgt gatttcttcc agcggctggc agaaatccag
     2161 tttgagtccg gctatcccta catcatgtat gaagacacgg taaaccgtgc taaccctgtc
     2221 gccgggcgca taaatatgag taatctctgc tcagagattt tgcaggtcaa ctgtgcctca
     2281 gagtatgacg agaatctcga ctatgcccgc acaggccatg atatctcctg caatttaggt
     2341 tcgttgaata ttgcgcacac catggattcc cccgattttg cccgcacggt agagactgcc
     2401 gtgcgcggtt taacggcggt gtcagatatg agtcatatcc gcagcgtgcc gtccatcgaa
     2461 gccggaaatg ccgcctcaca cgccatcgga ctggggcaga tgaatttaca cggctatctg
     2521 gcgcgagaag gcatcgctta tggttcgccg gaagcactgg atttcaccaa tctctatttc
     2581 tatgccatca cctggcacgc actgcgtacc tcgatgttgc tggcgcgcga acgcggtgaa
     2641 accttcgccg ggttcaaaca gtcacgttat gccagtggtg aatattttag ccaatatctg
     2701 caaggtaact ggcagccgaa aacggcgaaa gttggcgaac tgtttgcccg cagcggtatt
     2761 acgttaccta cccgtgagat gtgggcgcaa ctgcgcgacg acgtgatgcg ctacggcatt
     2821 tataaccaga atcttcaggc ggtgccgcca accggttcta tctcttatat caaccatgct
     2881 acgtcgagta ttcatccgat tgtggcgaaa gtagagatac gcaaagaggg caaaacagga
     2941 cgcgtttact accctgcccc gtttatgact aacgagaatc tggcgctgta tcaggatgct
     3001 tacgaaatcg gcgcagaaaa gatcatcgac acctacgccg aagcgactcg ccatgtcgat
     3061 caggggctgt cgctgacgct gtttttcccc gataccgcca ccactcgcga tatcaacaaa
     3121 gcgcagattt acgcctggcg caagggtatc aaaacgctct attacattcg cctgcgtcag
     3181 atggcgctgg aaggcactga aattgaaggc tgcgtctcct gtgcacttta aggaatatct
     3241 atgaaactct cacgtatcag cgccatcaac tggaacaaga tatctgacga taaagatctg
     3301 gaggtgtgga atcgcctgac cagcaatttc tggctaccag aaaaggtgcc gctgtcgaac
     3361 gatattcctg cctggcagac attaactgtc gtagaacaac aactgacgat gcgcgttttt
     3421 actggcctga cgctgctcga cacgctgcaa aatgttatcg gcgcgccttc gctgatgccc
     3481 tatgcactca cgcctcatga agaagcggta ttatcgaata tcagctttat ggaagcggtt
     3541 catgcccgct cttacagttc gattttctcg acgctgtgtc agaccaaaga tgtcgatgcc
     3601 gcctacgcct ggagtgaaga aaacgcaccg ttgcagcgaa aagctcagat tattcagcaa
     3661 cattatcgcg gtgatgatcc gctgaaaaag aaaatcgcca gtgtgtttct tgaatctttt
     3721 ttgttctatt ccggtttctg gctgccgatg tatttttcca gccgcggaaa gctgaccaat
     3781 accgcagatc tgatccgtct gattatccgc gatgaagcag tccacggtta ctacat
//
LOCUS       DAJTYD010000235         3819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTYD010000235 DAJTYD010000000
VERSION     DAJTYD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.239"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..295)
                     /gene="ymfL"
                     /locus_tag="OO585_003760"
     CDS             complement(<1..295)
                     /gene="ymfL"
                     /locus_tag="OO585_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCR6631190.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNAD"
     gene            complement(339..539)
                     /locus_tag="OO585_003761"
     CDS             complement(339..539)
                     /locus_tag="OO585_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6631191.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            630..1304
                     /locus_tag="OO585_003762"
     CDS             630..1304
                     /locus_tag="OO585_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6631192.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            2834..3370
                     /locus_tag="OO585_003763"
     CDS             2834..3370
                     /locus_tag="OO585_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HCR6631193.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3361..3723
                     /locus_tag="OO585_003764"
     CDS             3361..3723
                     /locus_tag="OO585_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631194.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGDWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
BASE COUNT         1182 a          820 c          808 g         1009 t
ORIGIN      
        1 tatcggcgtt gtccacatcc tcgatgtcag gaagagacac aaagacgcca tttgcagact
       61 gcgccacagc atcagcaatg aagtgagtgc caccagcacg ctgtaaaacc attgcccatc
      121 ccagcgggaa aatctgatcg ccatctgcac gaaggcggtt gaataaagcg ttttctgtta
      181 catcgagcca gtcagccgct tcagcgtaac cacccggcaa ctttgcgata gtttttctga
      241 cagctttcac gtaccactca ggctgttttt ctattttcca gtgatgctta cccacgatta
      301 gcctcatcgt tctgtggtta aaaattgaaa gtgttctgct aatctttcgg atagatatcc
      361 ggtcttaagt cagatttcgt aattgcacct gacgtgcatt gctcaagttt tttagccagc
      421 acaaaactgg cttttttata gccattgaaa accagccgta agtagccttg tgttgagcca
      481 acttttccgg ccaactcacc ctgctgttct ttggttaaag agtcccaata cgctttcata
      541 caatatgtac ctccagtgta cacatcacat gattgaaatg aaccttcaag atacttgtac
      601 cttagcggta caagggtttt aatttcgtta tgaaaacaat ccatgacatc cggcggtcta
      661 acgccagaaa actgagagat ggtgttggcg ggaattcttc ctttgccact atgattgatc
      721 gcgagccaac ccagaccagc aggtttatgg gagatggtgc tactaaaaat atcggtgaca
      781 gcatggcacg acacattgaa aaatgtttcg acctgcctat cggatggctc gatcaagaac
      841 accagacaac gaacatcaca aaaaaacctg atgtttcaat cactaacaaa caaataacgt
      901 tagtccctgt catatcatgg gtacaggccg gagcatggaa agaagttggc tattctgagg
      961 ttgatttgag cacagcagaa acttatccct gccctgtacc ctgtggcgaa atgacttata
     1021 tcttgcgggt gattggtgat tcaatgattg atgagtaccg tccgggagac atgatttttg
     1081 ttgatcctga agtccctgct tgccacggtg acgacgttat tgcgttgatg cacgatacag
     1141 gcgaaaccac cttcaagcga ttgatagaag atggaacaca gcgttatctc aaagcattac
     1201 acccaaactg gcctgagccc tacattaaga ttaacggtaa ttgctctata attggcactg
     1261 tgattttctc gggaaaacca agaagataca aaataaaggc ctaatcaata tttatgaacc
     1321 tgcttcggca ggttttttta tacttgacaa tgtaccccat ggacacacaa tgtaccccca
     1381 tggacacaca atgtaccaaa acgaaacaac aaacaggcag gatgcccacg aagtagccgc
     1441 ctggggcata tgaagtccaa gatgattcgt tagcaacaaa acgcccatag gacgcttcgc
     1501 tctttaacaa tttgaatatt ttcagcgtca atgatctgtt tagctcccta caagtaaatg
     1561 tagcgtagca ccatgataaa tcctgctagt gtttagttaa aaatcgcgcg agagcaagaa
     1621 cgacgtgagg gcaggcaata ctgatagtgt tattgctatt agtttatcaa aaatatttag
     1681 acgacccaga tgaatatttt taatacatac tccccctaat ttaacaggag taaatgttga
     1741 tgaataacga gacatctata ctcaacaatt gtatgatatg aacaattata taacataatg
     1801 taggaaaaat aaaaatggat tggcatgacc ttatatcaaa ggtaattatc ggacttgtta
     1861 caggctcaat cgcagcttac ttaactgcgc gatatgcact cggccgttat tataaagaaa
     1921 aatggtggga caaaagaata gatttgtata cgtaattagt tgactgtgtt tatcttctaa
     1981 aaaacacagc tgactattgg tataaacatg agttacatca cgcttcacaa ttcccaacct
     2041 actttagtta caaatcaaaa gaagaaaatg aaatccttag caaatctttc tctaatgagc
     2101 ttgaaaaact cagtagaata ggtgagctat cttcattatt cttaacagaa gagtgtgggg
     2161 ataaaatccg ttcattcatt gaaaatgaag aaaatctatt tatacaatgg gtgtttgata
     2221 gtatagagtt gatagatgcc cacaagtcaa gacttgataa attaaaagta ttactagacg
     2281 atattatctc tgaagcaaag agagagctta aggtcaataa aaaacataac agcctaaaga
     2341 atataaagtg gtccataact atgccagacc aaaaaaatga tgagccaatt aattatcacg
     2401 acaatcatta aaacacttta gaacccgctg ggcgggttct attcaaatat actttgcaca
     2461 tcacaaataa aactataacg tccttcttct tttcctaata tcttatatct acttatttat
     2521 aagcggctta cagatcttca acagataaac ctaatgaagg cgacaatgtt ctgatgaata
     2581 gccacctttt tactcaactg aaagcgcgtt ctgtcccttg tcattaagtg ccagttcgtt
     2641 aagtccaaaa cctccggaac gcgctttcaa ttgagtgggg aaaataactg gcgatggcag
     2701 ccgcccgttg cactaagtgc ctctggatgg ggcatttact gaaacgaaac caaaactttt
     2761 aatcgcctta tggcgaggga ttcgtgcaac caaaaatcgt gcggtgcagc gcaccaatta
     2821 aggagaacta acgatgagtt ttatccagac tcttaccggt aagaagttca attatatcaa
     2881 ctccaccatt gacgatattt atatcgagga tatcgccggt gcgctttcaa atatctgccg
     2941 cttcgcggga cacttgccgg atttttacag cgtggcgcag cattccgttc tgtgcagtca
     3001 acttgtggcg ccagagttcg cctttgaagc gttaatgcat gatgcaacag aagcgtattg
     3061 tcaggacatc cccgcgcaac tgaaacgcct tcttcctgac tataaacgga tggaagaaaa
     3121 aatagacgcc gtaatccgtg agaaatacgg gttacccccg gttatgagca cgcccgtgaa
     3181 atatgccgat ctcatcatgc tggcaaccga acgccgcgat cttgggcttg atgatggctc
     3241 tttctggcct ctactagaag gtatcccagc aacagagatg ttcaaagtta ttccactgtc
     3301 gccaggccat gcctacggga tgtttatgga acgttttaac gaattatcgg agttacgcaa
     3361 atgcgcatga atgttttcga aatggaaggg tttcttcgcg ggaaatgtgt accgcgagat
     3421 ctgaaagtga atgaaacgga tgctgaatac ctggtgcgta aattcgatgc gcttgaagct
     3481 aaatgtgcgg cactggaaaa caaagtaata ccagtgtcaa ctgaactgcc accagcaaat
     3541 gaaagtgttt tgttattcga tgctaacgga gaaggctggc taattggctg gcgttctctc
     3601 tggtacacct ggggacaaaa agaaaccgga gactggcagt ggacatttca ggtcggggac
     3661 cttgaaaacg tcaatatcac tcactgggca gtaatgccaa aagcaccgga ggctggagca
     3721 taatgaccac ttttaccgac aaagaactga ttaaagaaat taaagagcgt atcagcagcc
     3781 ttgacgtgcg agacgatatt gagcgccgtg cttatgaaa
//
LOCUS       DAJTYD010000236         3786 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTYD010000236 DAJTYD010000000
VERSION     DAJTYD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.240"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..442)
                     /locus_tag="OO585_003765"
     CDS             complement(<1..442)
                     /locus_tag="OO585_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6631195.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFA"
     gene            complement(597..1223)
                     /gene="dsbA"
                     /locus_tag="OO585_003766"
     CDS             complement(597..1223)
                     /gene="dsbA"
                     /locus_tag="OO585_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6631196.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1240..2226)
                     /gene="srkA"
                     /locus_tag="OO585_003767"
     CDS             complement(1240..2226)
                     /gene="srkA"
                     /locus_tag="OO585_003767"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6631197.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2303..2572)
                     /locus_tag="OO585_003768"
     CDS             complement(2303..2572)
                     /locus_tag="OO585_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6631198.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2642..3226
                     /gene="mobA"
                     /locus_tag="OO585_003769"
     CDS             2642..3226
                     /gene="mobA"
                     /locus_tag="OO585_003769"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6631199.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3208..3735
                     /gene="mobB"
                     /locus_tag="OO585_003770"
     CDS             3208..3735
                     /gene="mobB"
                     /locus_tag="OO585_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6631200.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1016 a          899 c          871 g         1000 t
ORIGIN      
        1 acgcgaatcg caatgtcgct tccacgggcg atttattttt cgcggcgata aatagcggtt
       61 gtgtcagttc attattcact aagttcactt ttgccgctgc cagtgctggg atgacattac
      121 catgcattaa cattgtgatg ggcaacggac cgtgctcaac atccacatca aatgcaactt
      181 tctggcctgg cttgatattg agatcgggtg caccgttatc aaatgaaatt tgcatttgaa
      241 aatgggaatt aaaaaagcct ttgtcaaagt ttttataact taacgtcata tcgtaggcat
      301 gttcaccttt ctttttagca tcgttaaaat ctttaataaa tttttcgaca ccaggctgaa
      361 tttgcgtacc tgtgtaccat atgccaccac cccagattac ggctaacgca acaataacac
      421 ctgtagctga cttacgtatc atataaatat tccctgataa aacagcaaaa ctttgaatat
      481 ccacttatgc tgaaataatg tccagcggca ggatgtatta tcagaatgta tttatattta
      541 ttaaataata aaaaaagccc gtgaatattc acgggcttta tgtaatttac attgaattat
      601 tttttctcgg acagatattt cactgtatca gcatactgct gaacaaaaac atccatattg
      661 ctggtatcca taccctgcgg attcagctga tatttaccgt taacaaacat cgccggaaca
      721 ccgcgcaatt gcacgtcagc tgcagctttt tcctgctgag ctaccagaga tttcaccacg
      781 aagctgttcc acgccgcgtc gtactcttca cctttaatac ctgcgttgat aaatacatcg
      841 cggatatcag aagcagaacg aatggtctgg gttttctgta cgccttcaaa cagcggaaca
      901 gtcactttgt cttccacgcc cagcgccatc gccacagccc atgcctgagt cagatctttg
      961 cccaggtcac cacccatgaa gttgacgtgg tatttagtca tcttcacgcc ttccggcagt
     1021 tttttcttca cattatcaga aatatgcaga acttcttcaa actgatagca gtgcggacag
     1081 aagaaagaga aaaactccag cacttgcggc gcgccagcta ccggtttttc cagggtagtg
     1141 tactgtttac catcttcata ctgcgccgcc gatgcgctaa acgctaaaac taaaccagcc
     1201 agcgccagcc aaatcttttt catgatctac tctctccgat taatacatag gtgttaattg
     1261 taaagggggt tcttgtagaa cttttgcctg ttctataaaa gtcgccgttt gtcgcagcca
     1321 gtaatcttcc ccggttaacc acgggaaatt tttcgggaac gcgggatcag cccaacgccg
     1381 cattagccag gcaagataat aaaccaaacg catggcgcgt aaaggttcaa tcagtccgat
     1441 ttcagcggtg tcgaactcgc taaattcttc ataagcttca ataatagttt ccagttgcat
     1501 ccgctgctcg gctttatcgc cattgagcaa catccacaaa tcctgaatag ctggaccatt
     1561 acgtgcatca tccagatcaa caaacattgg accatcgcgc cagagaatat tcccggcgtg
     1621 gcagtctcca tgtaagcgca gaacggtgga atcttcccgc cagtgcgctg taacggcggc
     1681 gatcagctca tctgtcgctt tcaggaatgc cgctttcaac ccggaaggta tcagtgtagc
     1741 gtcctcaaac agcttgcgtg gctcaatgag atattcattc agaccgatgg tcgggcgatg
     1801 gataaaaagc tgtttgcgcc ccgtctggtg catacgccct aaataacgcc caaccgcttc
     1861 catctgatcg atattatcag cttcgaactg gcgaccaccg acgcttggaa aaacagcgaa
     1921 ataaaatccc tgatgattca ataaagtctg accgttaaag gccacaggtg ctgcgaccgg
     1981 aacttcatca tttaccaact gcaacgcaaa ttgatgttct tcgaggattt gatcggctgt
     2041 ccaacgttca gggcgataaa atttgacgac aatacgtcga cgatcttcgt cctgaaattg
     2101 atagacacgg ttttcatagc tgttaagcgg ggtaagaccg gaatccaccc ggatcccttg
     2161 ctcaaataga gcatccatga tggtatccgg gtgtagtgtc tggaaagtaa aagcgctgtt
     2221 gttcatccca tcatccggaa aacattacga atgatgaagg atatcatttc acgatgattt
     2281 tggtggcgcc gcttacaagc ttttactctt taattacgcc gcgagcgcgt aacagcgccg
     2341 ttttgaagtc ttcttcataa tctttctgca aacccgggat caccgcatct ttcgcggaat
     2401 cacgcatttt caggtgatag atcagaatgt catccgtcaa atccgccagt tcgccgtcaa
     2461 aacctgactc tttcgccagt ttctgcaaaa attgcaacag gttaaggtct ggctcttttt
     2521 gccaggctgg ctggaggaat tcaataactt cattcagacg tttacatttc atagtgatgc
     2581 tccttactct tgagacagac acgttagcag ggtcaatccc acaataaaag aggcgatatc
     2641 ggtgaatctg atgacgacga taacaggcgt tgtgctggca ggcggtaaag ccagacgaat
     2701 gggtggcgta gataaaggat tgcttgaatt aaacggcaaa ccattatggc aacatgtcgc
     2761 tgacgcgctt atgacgcagc tctctcacgt cgtggttaat gccaatcgtc atcaggaaat
     2821 ctatcaggca agcggtctga aagtgattga agattcactg gcggattacc caggccctct
     2881 ggcaggaatg ctttcagtaa tgcagcagga agctggtgag tggtttttgt tttgcccgtg
     2941 cgatacgcct tacattcccc ctgatttagc agcccggctt aatcatcagc gcaaagatgc
     3001 gcctgtcgtg tgggtccatg acggtgaacg cgatcacccg actattgctc tggtaaaccg
     3061 cgctattgag cctttattac tggaatatct gcaagcagga gaacgccggg taatggcatt
     3121 tatgcgtctg gctggcggtc atgcggttga tttcagcgat cataaagatg catttgttaa
     3181 cgtgaatacg ccagaggagc ttgcccgatg gcaggaaaaa cgatgatacc gttactcgcc
     3241 tttgccgcgt ggagtggtac cggaaaaact acgcttttga aaaaactgat cccggcatta
     3301 tgcgccagag ggatccgtcc agggctgatt aagcatacgc accatgatat ggatgttgat
     3361 aagccaggca aagatagcta tgagctgcgc aaggctggcg cggcacaaac catcgttgcc
     3421 agccagcaac gatgggcctt gatgacggaa acaccagacg aagaagagct ggatctacag
     3481 tttctcgcaa gtcgaatgga tacctcaaag ctggatttga ttctggtcga agggtttaag
     3541 catgaagaga tcgcaaagat tgtgctgttt cgcgatggag ccagacatcg acctgaagaa
     3601 ttagtgatag acaggcatgt tattgctgta gccagtgatg tgccgcttaa tcttgatgtc
     3661 gcattactgg atattaatga tgttgagggg ctggctgatt ttgtggtgga gtggatgcaa
     3721 aagcagaacg gataagcaag agtaggctgg atcagacgca gccagtgtcg catccgacct
     3781 ttttct
//
LOCUS       DAJTYD010000237         3723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTYD010000237 DAJTYD010000000
VERSION     DAJTYD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.241"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..329
                     /locus_tag="OO585_003771"
                     /pseudo
     CDS             1..329
                     /locus_tag="OO585_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            446..595
                     /locus_tag="OO585_003772"
     CDS             446..595
                     /locus_tag="OO585_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631201.1"
                     /translation="MSHYKVQQMLSCQKRRQKVQPVMQSGMQRPQQKKPGSQQKAHSQ
                     RNKAG"
     gene            628..1291
                     /locus_tag="OO585_003773"
                     /pseudo
     CDS             628..1291
                     /locus_tag="OO585_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            1377..1889
                     /locus_tag="OO585_003774"
     CDS             1377..1889
                     /locus_tag="OO585_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631202.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            1965..2177
                     /locus_tag="OO585_003775"
     CDS             1965..2177
                     /locus_tag="OO585_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6631203.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(2319..2644)
                     /locus_tag="OO585_003776"
                     /pseudo
     CDS             complement(2319..2644)
                     /locus_tag="OO585_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(2780..3028)
                     /locus_tag="OO585_003777"
     CDS             complement(2780..3028)
                     /locus_tag="OO585_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6631204.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3383..>3723)
                     /locus_tag="OO585_003778"
     CDS             complement(3383..>3723)
                     /locus_tag="OO585_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6631205.1"
                     /translation="KVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRG
                     IDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARV
                     RSVEEILHAL"
BASE COUNT         1006 a          795 c         1032 g          890 t
ORIGIN      
        1 tcagaaaatc cggatgaagc cgggcgttac agtatggacg ttgagtatgg tcagtacagc
       61 gttattctgt tggtggaggg attcccgccg tcacatgccg ggactatcac cgtgtatgaa
      121 gattctcaac ccggtacgct gaatgatttt ctcggtgcca tgactgagga tgatgcccgt
      181 ccggaggcac tgcgtcgctt tgaactgatg gtggaagagg tggtgcgtaa cgcagaggag
      241 gcgaagaaga atgccggaga ggcggagacg tcagcgagga atgccggcat atcagccagt
      301 caggcagaag agagcgctgc aaatgctgac acttcagcag gggatgcatc ggagtcagcc
      361 cggcaggcgg cagaaagtgc agcctcagca aagcagtcag aggaggcgtc ctcgtcctcg
      421 gcctctgcgg ccgctcaaaa agccagtgag tcattacaaa gtgcaacaga tgctgagttg
      481 tcaaaaaaga cggcagaaag tgcagccggt aatgcagtca gggatgcaac gaccgcaaca
      541 gaaaaagccc gggagtcagc agaaagcgca cagtcagcgg aacaaagcag gatagcggcg
      601 gaagaggccg taaaccgaat ccccaccgtg gtgggacctc ccgggccaaa gggggaaccg
      661 gggcccgcgg gtcctcaggg gccgaaggga gataaaggag agcgtggcga caccggcccg
      721 gcaggggcca aaaggtgaca aaggcgatcc gggagtggct ggaccagcag gtccggcagg
      781 tgcgccgggg ccgaaaggcg ataaaggtga tccgggagta gcaggtccag caggtccggc
      841 ggggccacag ggaccgaagg gagacacagg agcccccggg caaggaacag aactgcttac
      901 tactgccaat acatggactc aggcacaaac ttttaatggt ggtattaatg gcaatttgac
      961 ggtgaccgga aacggctcat ttaacgatat tcagatccgt tcggataaac gcaacaagcg
     1021 aaatctggta aaactggata atgcgttaga tcgtctggag gcacttactg gttatcttta
     1081 cgagatacag tactctgccg acggttggca aacgtcggtt ggtttaattg ctcaggatgc
     1141 acaaaaagca ttgcctgaac tggtaactga agacgcagac gttatatctg gtgaaaaacg
     1201 tctgcgtctt aactacaacg gcataattgc attgttagtc gagggcttta aaacacttcg
     1261 tcatgagatt aaagaactcc gggagaagta aacgacagct gttgtagttt ctggtttcta
     1321 ctgaatttaa attgtggggt gacactcacc ccacgcattc agaaggggga gatgagatgg
     1381 gggtaacatc gggatgggta ggctcttcgg ctaagagcga aacaggtgag cagtggatgg
     1441 gagccgccgg aaccaaactg ggactgagta aaccttttat gatgagccaa atggttgggc
     1501 gaactatggg ctgtaaaatt gcaactgagt actataaatg gaaatcctct gacaaggttg
     1561 ataactgggg cgcagttggc gctgactggc ctttagaaga aaaaagtaaa ggtacaatta
     1621 caaacgccgc aagctgcgga tcagggaggc tggtaggggc tgtcgttaca ctttctcact
     1681 ttttgacgaa ctctacaccg acagctgctg tttatttagc cggtggtaaa gcaggtaaca
     1741 tcaccgtaaa cgtaggtggt gctacacaaa ccatgattta tcagggcgtt gttagtgggt
     1801 tccagtatta ctggtcaggt tctgttagtt ccgctttcgt ggaggcaatg aaaaagacgg
     1861 gagtacccca ggatctaaaa attagttaaa tggtaaataa ataattttaa aaacttcacg
     1921 ttctacatac cggagacgcc ggatatatag gatattgttt taagttgcca gagaaatttt
     1981 tccggacgga tgctgataat aatgatgtgc caatgacggc agcatcatgg atggcactga
     2041 gtgaagcgac agagcaggcg atgtttgcga agggagtgga gattaataca cggcagttgc
     2101 agatgaaagc cgaggttgag gcgctgacag acctgaaggc gatccgcagt tatgttgtcg
     2161 gatggcctgc tggctgaata aaaaaacggg accacgacca gtcccggaac catgagtttt
     2221 agggtattag tttgttatca tagttagcgt gctaagtatg ccatgtcagg ttgattagtg
     2281 aagtgatgtt gttcgcattt ttgcacggcg gagaatattc agatttttgg aaatccatat
     2341 ttttcccgtg cgcgattaca tgcttcattt acgatgctat tttcgccgga cattgcgaat
     2401 tccctgcatg tggacggttt ttgtaaacag agcaatatgc gttttctccg gggggggccg
     2461 gcaagagcgc cacatcgggg atttttctga ttggtgccgc gcatacagcg atgaaaagga
     2521 gatatttgct cagtgagact gaccgaaacc tttccgccag catcgtcagc ttctgcccag
     2581 taaaaagaga cgcggaaaat gcacaacagg caccacacgt catgcatgga ttcagattgc
     2641 tcataattca ccagtacagc tataaatcgt aaagagaaca gcggtacatc gtatgtaaga
     2701 acgtaccgcg gctggctggt gaacttccga tagtgcgagt attgaatgat ttccagccgt
     2761 taccgatttt acgtgctaat tagtgaacaa accactcgtc agcagactcc caggtatctt
     2821 tcagagtttc ctgaacaaaa gttttagctg aatctttatc ggcggtgcgc gtaacagaaa
     2881 ggccatcgtt gctggtagct tttacgatca cctctacatc gtcataacgt ttactgatgc
     2941 gtcgggttaa ttcttccttt aacgcatcca cagcaccggt tggcatttta gtcatttttt
     3001 ctttggctat gcagatttca atacgcataa aagtccctct acactgtgtt tgtatacagt
     3061 atcattttta actgtatgga taaacagtat taagggtgaa ggaaagtgat tttttggggc
     3121 atgcatgggg caaaaatatt tgttgtgggg cattttgggg catgattggg acactttgtt
     3181 acatatgaac tttgccgatt tttatatgac atggaaatac aacgctttga ttcttctaaa
     3241 taaaaaacat gctcttgggc gttctttagt gatttttaaa aattccgtat cacgcagtta
     3301 aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt aggatgcgac
     3361 cattgcggta gaccgatgta aatcataacg cgtgaaggat ctcttccacg ctacgcacac
     3421 gggcgatgcg cgggtagata tgattaatgc tgttattgtg ttgctcggcg ctggcggcac
     3481 tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg cgggcggtgg
     3541 attcaacacc gatattggtc gagatcccac ataacactat tgtatcgata cctcggcggc
     3601 gcagttgcaa ctccagatcc gtaccgtaaa acgcacccca ctgacgtttg atgatttcga
     3661 tatcactgtc agttgcacct aatgcagctg gatgttgcca ccagttttcg ggcaacactt
     3721 tag
//
LOCUS       DAJTYD010000238         3619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTYD010000238 DAJTYD010000000
VERSION     DAJTYD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.242"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..238
                     /locus_tag="OO585_003779"
     CDS             <1..238
                     /locus_tag="OO585_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6631206.1"
                     /translation="SGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALF
                     SLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(216..839)
                     /gene="tetR(B)"
                     /locus_tag="OO585_003780"
     CDS             complement(216..839)
                     /gene="tetR(B)"
                     /locus_tag="OO585_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6631207.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            921..2126
                     /gene="tet(B)"
                     /locus_tag="OO585_003781"
     CDS             921..2126
                     /gene="tet(B)"
                     /locus_tag="OO585_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6631208.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2239..2832)
                     /gene="tetC"
                     /locus_tag="OO585_003782"
     CDS             complement(2239..2832)
                     /gene="tetC"
                     /locus_tag="OO585_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6631209.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2965..3336
                     /locus_tag="OO585_003783"
     CDS             2965..3336
                     /locus_tag="OO585_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6631210.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3346..>3619)
                     /locus_tag="OO585_003784"
     CDS             complement(3346..>3619)
                     /locus_tag="OO585_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6631211.1"
                     /translation="SSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQANTVRNR
                     NVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT          977 a          674 c          750 g         1218 t
ORIGIN      
        1 aagtggtatt caatattttc atgaaatggg aattgacgtt ccttccaaac attcacgtaa
       61 aatctgttgt gcgtgtttag attggagtga acgccgtttc catttaggtg ggtacgttgg
      121 agccgcatta ttttcgcttt atgaatctaa agggtggtta actcgacatc ttggttaccg
      181 tgaagttacc atcacggaaa aaggttatgc tgcttttaag acccactttc acatttaagt
      241 tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg ctctgcacct
      301 tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc agtagtaggt
      361 gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta
      421 aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat
      481 aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa
      541 tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta
      601 cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct
      661 aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat
      721 gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg
      781 acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa tctagacatc
      841 attaattcct aatttttgtt gacactctat cattgataga gttattttac cactccctat
      901 cagtgataga gaaaagtgaa atgaatagtt cgacaaagat cgcattggta attacgttac
      961 tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta cgtgaattta
     1021 ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat gcgttaatgc
     1081 aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc
     1141 tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt tcaagtgcgc
     1201 tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact ggggctgtcg
     1261 cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg ttcggttggt
     1321 taggggcaag ttttgggctt ggtttaatag cggggcctat tattggtggt tttgcaggag
     1381 agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc gctttccttg
     1441 tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat accgaagtag
     1501 gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg cccattttgt
     1561 tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg tgggtgctat
     1621 ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta gcgggtcttg
     1681 gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact aaatggggcg
     1741 aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc tttttagcgt
     1801 ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt ggtgggatcg
     1861 ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt
     1921 tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca ttactgtttg
     1981 ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt attggtttag
     2041 cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct caagctcagg
     2101 ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa
     2161 tataatgacc ctcttgataa cccaagaggg cattttttac gataaagaag atttagcttc
     2221 aaataaaacc tatctatttt atttatcttt caagctcaat aaaaagccgc ggtaaatagc
     2281 aataaattgg ccttttttat cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg
     2341 cataaaccag ccattgagta agtttttaag cacatcatca tcataagctt taagttggtt
     2401 ctcttggatc aatttgctga caatggcgtt taccttacca gtaatgtatt caaggctaat
     2461 tttttcaagt tcattccaac caatgatagg catcacttct tggataggga taaggttttt
     2521 attattatca ataatataat caagataatg ttcaaatata ctttctaagg cagaccaacc
     2581 atttgttaaa tcagtttttg ttgtgatgta ggcatcaatc ataattaatt gctgcttata
     2641 acaggcactg agtaattgtt ttttattttt aaagtgatga taaaaggcac ctttggtcac
     2701 caacgctttt cccgagatct catctattga aacagcttga tagccttttt caacaaacaa
     2761 tattcgtgct gagttaacca gtgattgata ggtactctta aaattttctt gttgatgatt
     2821 tttattttcc atgatagatt taaaataaca taccgtcagt atgtttatgg tatcatgatg
     2881 atgtggtcgt gacaatctta agaacattta ggttatttta tgtatattga acagcattct
     2941 cgctatcaaa ataaagctaa taacatccaa ttaagatatg atgataagca gtttcataca
     3001 acggttatca aagatgttct attatggatt gaacataatt tagatcagtc tttactgctt
     3061 gatgatgtgg cgaataaagc gggttatacc aagtggtatt ttcagcggct gttcaaaaaa
     3121 gtaacagggg tcacactggc tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt
     3181 gagttgaggt tgacgaaaaa aactatcctt gagatcgcat taaaatatca atttgattcc
     3241 caacaatctt ttacacgtcg atttaagtac atttttaagg ttacaccaag ttattatcgg
     3301 cgtaataaat tatgggaatt ggaggcaatg cactgagaga tcccctcata atttccccaa
     3361 agcgtaacca tgtgtgaata aattttgagc tagtagggtt gcagccacga gtaagtcttc
     3421 ccttgttatt gtgtagccag aatgccgcaa aacttccatg cctaagcgaa ctgttgagag
     3481 tacgtttcga tttctgactg tgttagcctg gaagtgcttg tcccaacctt gtttctgagc
     3541 atgaacgccc gcaagccaac atgttagttg aagcatcagg gcgattagca gcatgatatc
     3601 aaaacgctct gagctgctc
//
LOCUS       DAJTYD010000239         3596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTYD010000239 DAJTYD010000000
VERSION     DAJTYD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.243"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..307)
                     /locus_tag="OO585_003785"
     CDS             complement(<1..307)
                     /locus_tag="OO585_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6631212.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     "
     gene            complement(438..1802)
                     /gene="sdaA"
                     /locus_tag="OO585_003786"
     CDS             complement(438..1802)
                     /gene="sdaA"
                     /locus_tag="OO585_003786"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6631213.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(1986..2564)
                     /locus_tag="OO585_003787"
     CDS             complement(1986..2564)
                     /locus_tag="OO585_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6631214.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(2568..>3596)
                     /gene="pabB"
                     /locus_tag="OO585_003788"
     CDS             complement(2568..>3596)
                     /gene="pabB"
                     /locus_tag="OO585_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6631215.1"
                     /translation="YDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLL
                     SHNDVNARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVN
                     LAQRFHATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTR
                     PIKGTLPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELF
                     VVEPFPAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRR
                     NAWCGSIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVT
                     KILRQLEK"
BASE COUNT          893 a          938 c          920 g          845 t
ORIGIN      
        1 gcaccaggcc aatggatcgc acagtttgga gttttgccgc ctgtttacgt aatggcaaat
       61 gggcgacaga gcagggcaga ccaatcagcg gaagcaagac ttcgctacct gcctgtaggg
      121 gaagcagtac tttatctaat tcctctacag cgtgattggc gaattgtact acccgttgtt
      181 gatttaagtt acgctgtgaa ataaatcgca cgctcagggt cgaagcgagc gtaacgagtg
      241 cgcaaatcgt acaaacaatc attcgattac gtcgataggt tttaatgatc cgttgtgctt
      301 tttgcatgcg ggccaccctg taagcgtatg gcgacaaacg cctgacgcaa cagtggatgt
      361 gtagtgggga atattacagc agacgagaaa gcgggtataa attcgcccat ccgttgcaga
      421 tgggcgagta agaagtatta gtcacactgg actttgattg ccagaccacc gcgtgaggtt
      481 tcgcggtatt tggcgttcat gtccttaccg gtttcgtaca tcgtttcgat gaccttatcc
      541 agcgagacgc gcggtgcact ggtgcggcgc agagccatcc gtgcggcgtt aatcgccttc
      601 acagaggcaa tggcattacg ctcaatgcac ggcacctgaa cctgccctgc aaccgggtcg
      661 caggttaaac caaggttgtg ttccatgcca atttccgccg ccacgcaaac ctgttccggg
      721 ctaccgccca gcagttctgc aagacccgca gcagccattg aacaggcaac acccacttcg
      781 ccctggcaac caacttccgc accggaaata gaggcgttca ttttatacaa tgcaccaatc
      841 gcgcccgctg ccataaagta acgggtatag atgtccgggc tgaccgattc aataaagtgg
      901 tcatagtaag ccagcactgc cggaacgata ccgcaggcac cgttggttgg cgcagttacc
      961 acacgaccac cggcggcgtt ttcttcgtta actgccagcg caaacatgtt tacccagtca
     1021 atgacattca tcggatcgtt agacagttta tcgctggaaa ccagcatccg gcgcagggca
     1081 gacgcacgac gtggcacgcg cagcgggcct ggcagtacac cttcggtgtt catcccgcga
     1141 tcgatacatg cctgcatggt ttgccagaca tgcgcgaaat actcgtcgat ctctttcttg
     1201 ctgtgcagcg ccagttcgtt ctgcatagcg agaccagaca gcgaatagcc ggtttcatta
     1261 cagtacgcga gcagttcggt ggcagatttg aacggatacg gcacgctcac ttcgttggca
     1321 gcatcctgac caaagtgttc ttcatcgacg ataaaaccgc cgccgatgga ataataagtt
     1381 ttgctgtaga cgacttcatc gccgttatag gcgtggattt gcataccgtt ttcatgcagc
     1441 ggcaggttgc cgttatgaaa acgcatcccg ttgtcgcgcg ggaaatccac ttcatgccgt
     1501 ccctgtgcca gcagcagacg ttcgcgctct tctacgtcgc gaataaaacc gggaatactg
     1561 tcgatatcca cggtggcagg ttcgttacct gcaagaccca taataatggc aatatcggtg
     1621 tggtggcctt tacccgtcag cgacagtgaa ccataaacgt ccacggcaac gcgagtaacg
     1681 ctatccagta agcctttttc gaccagatca tcgacgaact gtttacccgc cttcataggc
     1741 cctacggtat gggaagatga gggaccaatc cccaccttaa acatgtcgaa tagactaatc
     1801 acgataatac tcctgacaag gtgactggac ttccagtaac gaactaataa cagcgcatag
     1861 tgtaagaggg aacgcggcga ctggcttaac tattcacatg aattaaacta atcaaaaacc
     1921 gcgatttaac taatattgcg atgattgtct caagtagtgt tttgtagatg taaagataag
     1981 tatagtcagg gtttcacacc aatttgcagc gccagctcac gaattatgcc tgcggtcatt
     2041 ccccatacaa aatactgttc gtaccaggac agccataccc gatgtgaatc gccacggcgg
     2101 tagatatcta aagggtgata acgacccaga tgtaatgcct gggcgagcgg catttcaaac
     2161 accgccgaga cttcatcttc actggcgcga tacggcagat cgggcgggat aatgccgatc
     2221 actggggtta cctgatagcc agttacgcta tcgacgggcg gcagcacgcc gataacttca
     2281 acggcggaag gcggtatagc gacctcttct tcagcttcgc gcagcgcggc ggcgataact
     2341 gatgcgtccg tgtcatcgac tgcacctcca gggaatgcca cttgtccagc gtgtttacgc
     2401 agatgaattg aacgctgagt cagcaacaac cccggttgcg gtcgacggac gatggggatt
     2461 aacacggcag cctgacgatg atttagggtt tcccggttaa tttgcgggcg caaaagttga
     2521 aagcgagata aaaaatcatc aagcgtcagg ctacggtatt ccacgtctta cttctccagt
     2581 tggcgtaata tcttagtaac tttatcaaaa gtttcctgat attccgcttc ttcctggcta
     2641 tcggcgacaa ttccaccgcc cgcagagcag tatatttgtc cgttaatggc agtcagcgtg
     2701 cggatagtaa tgctggtatc catgttgccg caaaagctca aatagccaat gctgccgcac
     2761 caggcattac gtcgctgcgg ttccagttcg tcgataattt ccatagcccg tactttcgga
     2821 gccccggtta ttgagccacc aggaaaagct gcgcgcagca gatcgctggc gtgtaactgt
     2881 tctggtagtc gcgccgttat agtgctgacc agatgatgca cggcagggaa gggttccacc
     2941 acgaagagct ctggtacttt taccgaaccg gctacggcaa cacgaccgat atcattacgc
     3001 attaaatcga caatcatcag attttcggca cgatctttcg ctgagttcgc cagtttttct
     3061 gcttgtttgc tatcttcctg aggatcgggc aggcgtggta gcgtgccttt aatcgggcgg
     3121 gtctggattt cactattatc acaaagaata aaccgctctg gcgaaaggct taaaattgca
     3181 ccctgttcaa gacgtaaaaa agcgctaaat ggcgcgcggt tggcctgatt aagctgaagg
     3241 aatgcctgcc attcatcgcc agaataggtc gcatgaaaac gctgggcgag attcacctga
     3301 tagcaatcac cgctgtgcag atattcctgt acctggcgaa atttttcgcc gtactgctcg
     3361 cgggtcatat tggattgcca gtcggaagtg agcgtgaaat cttcctgcgg cgagaattgc
     3421 tggctttcca gccaggcccg acgggcattg acatcattat gactcagcaa agaaactgta
     3481 tgacgctggt ggtcgacaat gagcgcccaa tcgtagatac ccactgccat atccggcaga
     3541 acgatatctt gttccgcaat ttctggcagt gactcaaaac ggcggcccag atcgta
//
LOCUS       DAJTYD010000240         3592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTYD010000240 DAJTYD010000000
VERSION     DAJTYD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.244"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..2123
                     /gene="ybiO"
                     /locus_tag="OO585_003789"
     CDS             <1..2123
                     /gene="ybiO"
                     /locus_tag="OO585_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6631216.1"
                     /translation="PAHAVSIPGVTTTTTTDSTTEPAPEPDIEQKKAAYGALADVLDN
                     DTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEVSRHYGEALSARFGQ
                     LYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCALPLYRKMGLWARQKN
                     RERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRTIAFQQSLFLNAFAL
                     IEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIGYGLIVAVPIISNQV
                     NVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLAFFSLFIRAFALVWH
                     WLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSGMFSRWLAKTITLSP
                     HTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFDFWNWLQNGAGQKTV
                     DILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRTLLTLFRNALAVIIS
                     TITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGVFIQFENGMNTGDLV
                     TIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGIGSVVANYDVDRHED
                     ADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTLRVSFTTLPLKQWTV
                     RFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2183..3109)
                     /gene="rlmF"
                     /locus_tag="OO585_003790"
     CDS             complement(2183..3109)
                     /gene="rlmF"
                     /locus_tag="OO585_003790"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6631217.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3385..3592
                     /locus_tag="OO585_003791"
                     /pseudo
     CDS             3385..3592
                     /locus_tag="OO585_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
BASE COUNT          807 a          948 c          931 g          906 t
ORIGIN      
        1 cacctgccca cgcggtatcc atacccggcg ttacaaccac aacgacaacg gactcaacga
       61 ctgaaccggc cccggaaccg gatatcgaac aaaaaaaagc ggcctatggc gcactggcgg
      121 atgtgctgga taacgacacc tcgcgtaaag aattgatcga ccagttgcgt actgtcgccg
      181 cgacgccccc tgcggaaccg gtcccgaaga tcgtgccgcc gacgctggtt gaagagcaaa
      241 ccgtgctgca aaaggtcacc gaagtcagcc gccattatgg tgaagccctt tccgcccgct
      301 tcgggcaact ttatcgcaat attaccggct ccccgcataa gccgtttaat ccacaaacat
      361 tcagcaatgc gctgacccat ttttcaatgt tagcggtatt agtgtttggt ttttactggc
      421 tgattcgcct gtgcgcactg ccgctgtatc gcaaaatggg cctgtgggcg cggcaaaaaa
      481 atcgtgagcg cagtaactgg ttgcagcttc cggcgatgat tatcggggcg tttattatcg
      541 acctactgtt actggcactg acattatttg tcggccaggt attaagcgac aacctgaatg
      601 caggcagtcg caccatcgct ttccaacaaa gtttgtttct caacgccttt gccctgattg
      661 aatttttcaa agctgtacta cgcctgattt tttgcccaaa cgtggctgaa ctgcgcccgt
      721 tcacgattca tgacgagacc gcccgttact ggagtcgtcg cctgagctgg ttaagcagtt
      781 tgataggtta tggcctgatt gtggccgtgc cgattatctc taatcaggtg aatgtacaga
      841 taggtgcgct ggcgaacgtc atcattatgc tgtgcatgac cgtctgggcg ttgtacctga
      901 tctttcgcaa taaaaaagag attacccaac atttgctcaa ctttgcggag cattcgctgg
      961 cctttttcag cctgtttatc cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct
     1021 attttatcgt gctgtttttc ttttcgttgt tcgatccggg caacagcctg aaatttatga
     1081 tgggtgcaac ggtgcgcagc ctggcgatta ttggtatcgc agcgtttgtt tccggtatgt
     1141 tttcccgctg gctggcgaaa accatcaccc tctcgccaca tactcagcgt aactatccgg
     1201 agttgcaaaa acggctgaat ggctggcttt cggcggcgct gaaaacggcg cgtattctga
     1261 ccgtctgcgt ggcggtaatg ttgctgttga gcgcatgggg attgttcgat ttctggaact
     1321 ggctgcaaaa cggcgcgggg cagaaaaccg tagatatcct gatccgtatc gcacttattc
     1381 tttttttctc ggcggttggc tggacggtgc tcgccagttt gatcgaaaac cggctggctt
     1441 cggatattca tggccgcccg ctacccagcg cccgtacgcg taccctgctg acgctgtttc
     1501 gtaacgcgct ggcggtgatt atcagtacca tcaccatcat gattgtgttg tcggaaatcg
     1561 gcgtcaatat cgcgccattg ctggcaggtg ccggggcatt aggtctggct atctcgtttg
     1621 gttcgcaaac gctggtgaaa gatatcatta ccggggtgtt tattcagttt gaaaacggca
     1681 tgaacactgg agatttggtg actatcgggc cgttgaccgg cacagtggaa cggatgtcga
     1741 ttcgctccgt gggcgtgcgc caggataccg gggcgtatca catcattccg tggtcttcga
     1801 taaccacctt tgctaacttc gtccgcggta ttggttcggt agtggcaaac tacgatgttg
     1861 atcgccatga agatgctgat aaagccaatc aggcactgaa agatgcggta gcggaattga
     1921 tggaaaacga agaaattcgc gggctgatta ttggtgaacc gaattttgcc gggattgtcg
     1981 gcttaagcaa taccgcgttt acactgcgtg tttcgttcac cacgctgcca ctcaaacagt
     2041 ggacggtacg ctttgccctc gacagccagg tgaaaaaaca tttcgacctg gtgggcgttc
     2101 gcgcgccagt gcagacttat taggtgctgc ctactccggg cgcgaccccg gctgaaccgt
     2161 taccgccggg ggagccaacg ctttaacgct ggcgattgac aaaacggcgg cgctgctcgt
     2221 cgttcataaa ggtccaggca ataaagcgac tctgcttttg cccctgggcc atctcttttt
     2281 ttaccacctt caccgcgccc acgtcggtca gagcacgata caacggcggt aagttttcac
     2341 cacgagatac cagcgaggta aaccacatca cctgcttcgc gaagcctttg ctctcttcaa
     2401 tcatcttttt gataaaggcg acttcaccac cttcacacca caactcctgt tgctgaccac
     2461 caaagttcag tgcatcgtct ttgttcagcc ccaggttacg gcgtttacgc tcactacctg
     2521 cccgcgctgc agcggcggaa tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct
     2581 cgtttttatg gatgatgccg ttaaaaattg ccccactctc tttttgccga cgcagacgaa
     2641 tggcgcggtt aagccccgga ttagcactga taatcgcctg cgcactgctt aacgcctggc
     2701 tgctggtttc actaccggta aaacgccagc catattcatg tacgccaatc agcggataaa
     2761 tacagttcgc accaacgccg atatccagaa tgctggcatt cgctggaatt gttccgcttg
     2821 cttcagccag taaatcggca aggtgatgaa tataatccgc ccggccgggt actggtgggc
     2881 agagaaaacc gtcggggata tcccagttcg ctacggcgta aaaatgggcc agcaacgcct
     2941 tattgagcgc cttcaccgcc agcggattgg caaagtctac gctttgctcc ccggcgggtg
     3001 taagcgtgag gaattgcctg agttcaggat tgacctgaca aagcgtggcg agatcgtagc
     3061 ggctgtgatg acggttgcgc ggatgcaacc ccggtttctg ggcggacata gcattctcct
     3121 ttaagcagcg gcgtaagata cccgcataac gcgacagggt aaatatctgg ctgcgtttat
     3181 cattccccaa aaccttaaca tggctatggt ttatggcaat tcatcataaa aatcagcatt
     3241 atgcatgtca ttgatttgtc ttattttgta aaaatatatc gttcaaaaaa taagcacatc
     3301 tctgtcaatt ttttgcgtca tttgcatcgc gcaggcgacc tacacttatc aggcactacc
     3361 cgttgtattt atgaggatgt cattatgaaa aagtgcctca cactactgat tgccaccgtc
     3421 ctgagcagaa tctctctcac ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt
     3481 cagctacggc ccgcggtacc gtttcggcaa ctggcgcatc aaacctaagc gatctggagg
     3541 ataaactggc agaaaaagcg cgcgaacaag gcgcgaaagg ttatgtcatt aa
//
LOCUS       DAJTYD010000241         3578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTYD010000241 DAJTYD010000000
VERSION     DAJTYD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.245"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            4..516
                     /locus_tag="OO585_003792"
     CDS             4..516
                     /locus_tag="OO585_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6631218.1"
                     /translation="MATQLPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAV
                     IHIVPRLVGAKSGGVFQAVLGAAVMAVAIWMPGVGIMASNLLFSLGASMTLGGVAQML
                     APKARTPRTQTTDNGKQNTCFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEG
                     DGGQVVVIGR"
     gene            577..>3578
                     /locus_tag="OO585_003793"
     CDS             577..>3578
                     /locus_tag="OO585_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6631219.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFSVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMVWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTL"
BASE COUNT          818 a          891 c         1148 g          721 t
ORIGIN      
        1 gcactggcca cacagctccc ggcgtttcgt cagaaactga gtgacggctg gtatcaggtg
       61 cgcattgccg ggcgtgatgc aggtgaaacc gaattgtctg cccgtcttaa tgagccgctg
      121 gcaaatggtg cagtgatcca catagtaccg cgtctggtgg gagctaaaag tggcggtgtg
      181 tttcaggcgg tgctgggggc agctgttatg gcggttgcta tatggatgcc gggggtagga
      241 attatggcga gtaatctgct gttttctctc ggtgccagta tgacgcttgg cggtgttgca
      301 cagatgctgg caccgaaagc cagaactccc cgtacacaga caacggataa cggcaaacag
      361 aacacctgtt tctcctcact ggataacatg gttgcccagg gcaatgttct gcctgttctg
      421 tacggtgaaa tgcgcgtggg gtcgcgggtg gtatctcagg agatcagcac ggcagacgaa
      481 ggggatggtg gtcaggttgt ggtgattggt cgctgatgca aaatgtttca tgtgaaaccg
      541 cctgcgggcg gttttgtcgt ttatggagcg tgaggaatgg gtaaaggcag cagtaagggg
      601 cataccccgc gcgaagcgaa ggacaacctg aaatccacgc agttgctgag tgtgattgat
      661 gccatcagcg aagggccggt tgacggtccg gtggatggat taaaaagcgt gctgctgaac
      721 ggtacgccag tcctggacag cgaggggaag acaaactttt ccggtgttac ggtggtgttc
      781 cgcgccggcg agcaggagca gacaccgccg gaggggtttg aatcttccgg ctcagagact
      841 gtgctgggta cggaagtgaa atacgacacg ccgatcaccc ggaccatcac gtcggcaaac
      901 attgatcgtc tgcgcctgac cttcagtgtg caggcactgg tggaaaacac ctcaaagggg
      961 gaccggaatc cgtcggaagt ccgcctgctg gttcagatac agcgtaacgg tggctgggtg
     1021 acggaaaaag acatcaccat taagggcaaa accacttcgc agtatctggc ctcggtggtg
     1081 gtgggtaacc tgccgccgcg cccgtttaat atccggatgc gcaggatgac accggacagc
     1141 accacagacc agctgcagaa caaaacgctc tggtcgtcgt acaccgaaat catcgatgtg
     1201 aaacagtgct acccgaacac ggcgctggtc ggcgtgcagg tggattcaga gcagttcggt
     1261 aaccagcagg tgagtcgcaa ttatcatctt cgcgggcgca ttctgcaggt gccgtcgaac
     1321 tataacccgc agacgcggca atacagcggt atctgggacg gaacgcttaa gccagcatac
     1381 agcaacaaca tggtctggtg tcggtgggac atgctcactc atccgcgcta cggcatgggg
     1441 aaacgtcttg gtgcggcaga tgtggacaaa tgggcgctgt atgtcatcgg ccagtactgc
     1501 gaccagtcag tgccggacgg atttggcggc acggagccgc gcatcacctg taacgcttac
     1561 ctgaccacac agcgtaaggc gtgggatgtt ctcagtgatt tctgctcggc gatgcgctgt
     1621 atgccggtat ggaacgggca gacgctgacg ttcgtgcagg accgaccgtc ggataaagtg
     1681 tggacctata accgcagtaa tgtggtgatg ccggatgatg gcgcgccgtt ccgctacagc
     1741 ttcagcgccc tgaaggaccg ccataatgtc gttgaggtga actggattga tccggacaac
     1801 ggtcatgaga cggcgacaga gcttgtggag gacgcgcagg ccattctccg ttacggtcgt
     1861 aacgtcacga agatggatgc ctttggctgt accagccggg gacaggcgca ccgcgccggg
     1921 ctgtggctga ttaaaacgga actgctggaa acgcagaccg tggacttcag cgtcggcgca
     1981 gaagggcttc gccatgtacc gggtgatgtc attgaaatct gcgatgatga ttatgccggt
     2041 atcagcaccg gtggtcgcgt actggcggtg aacagccaga cccggacgct gacgctcgac
     2101 cgtgaaatca cgctgccgtc ctcctgtacc acgctgataa gcctggttga cggaaatggc
     2161 aatccggtca gcgtggaggt tcagtccgtc accgacggcg tgaaggtgaa agtgagccgg
     2221 gttcctgacg gcgttgcaga atacagcgtg tgggggctga agctgccgac gctgcgccag
     2281 cgcctgttcc gctgcgtgag tatccgtgag aacgacgacg gcacgtatgc catcaccgcc
     2341 gtgcagcatg tgccggaaaa agaggccatc gtggataacg gggcacactt tgacggtgac
     2401 cggcgcggca cggtgaatgg tgtcacgccg ccagcggtgc agcatctgac cgccgaagtc
     2461 accgcagaca gcggggaata tcaggtgctg gcgcgatggg acacgccgaa ggtggtgaag
     2521 ggtgtgagct tcctgcttcg cctgaccgtg gcagcggacg acggcagtga gcggctggtc
     2581 agcacagcca ggacgacgga aaccacatac cgcttcaggc agctggcgct ggggaattac
     2641 agtctgacag tccgggcggt aaatgcccgg gggcagcagg gcgatccggc gtcggtatcg
     2701 ttccggattg cggcaccggc agcgcctgtc actattgaac tgataccggg gtattttcag
     2761 ataacggcgg tcccgaaact ggctgtatac gacccgacgg tgcagtttga gttctggttt
     2821 tcggaaaagc ggattgcgga tatcaggcag gttgaaacca gcgcccgcta tcttggcacg
     2881 gcgctgtact ggatagccgc cagtatcaat atcaggccgg gccatgatta ttatttttac
     2941 gttcgcagtg tgaacaccgt tggcaaatcg gcatttgtgg aggccgtcgg tcgggcgagc
     3001 gatgatgcgg aaggttatct gagtttttat aaagggttga tcaataacac gcatctcggc
     3061 aaggagttgt ggacgcagat tgataacggt cagcttgcgc cggacctgac tgaaatcagg
     3121 acgtccatta cgaatgtcag caatgaaatc acgcaaaccg tcaataaaaa actggaaaat
     3181 cagagtgctg caatccagca gatacagaaa gttcaggttg atacaaataa taacctgaac
     3241 agcatgtggg ctgtgaagct gcaacagatg aaggacggac gcctttatat tgcgggtatc
     3301 ggtgccggta ttgagaatac gccagcagga atgcagagtc aggtgctgct ggcggcagac
     3361 aggattgcga tgattaatcc tgcgaatggc aacacaaagc cgatgtttgt tggtcagggc
     3421 gatcagatat tcatgaacga cgtgttcctg aaacgcctga cggctccgac cattaccagc
     3481 ggcggtaatc ctccggcatt ttccctgaca cctggcggac ggctgacggc gaaaaatgcc
     3541 gatatcagcg gtaacgtgaa cgcgaactcc gggacgct
//
LOCUS       DAJTYD010000242         3564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTYD010000242 DAJTYD010000000
VERSION     DAJTYD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.246"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..340)
                     /locus_tag="OO585_003794"
     CDS             complement(<1..340)
                     /locus_tag="OO585_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR6631220.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPRAVFWMWVAAFIG
                     MATSFAECSLA"
     gene            619..1572
                     /gene="tal"
                     /locus_tag="OO585_003795"
     CDS             619..1572
                     /gene="tal"
                     /locus_tag="OO585_003795"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6631221.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            1687..2274
                     /gene="mog"
                     /locus_tag="OO585_003796"
     CDS             1687..2274
                     /gene="mog"
                     /locus_tag="OO585_003796"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6631222.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2309..2875)
                     /gene="satP"
                     /locus_tag="OO585_003797"
     CDS             complement(2309..2875)
                     /gene="satP"
                     /locus_tag="OO585_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6631223.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3024..>3564)
                     /locus_tag="OO585_003798"
     CDS             complement(3024..>3564)
                     /locus_tag="OO585_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631224.1"
                     /translation="HRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
BASE COUNT          940 a          911 c          910 g          803 t
ORIGIN      
        1 gtgcaaggga acattcggca aacgaggtcg ccatgccgat aaacgcggca acccacatcc
       61 aaaagacggc tctaggtcca ccggtggtaa tagccagcgc aacgccggcc aggttgccgc
      121 tacccacgcg cgccgcaaga ctggtacaca atgactgaaa tgaggttaaa ccgcctggct
      181 gtggatgaat gctattttta agacttttgc caaactggcg gatgtagcga aactgcacaa
      241 atccggtgcg aaaagtgaac caacaacctg cgccgaagag caggtaaatc attaccgatc
      301 cccaaaggac gctgttaata aaggagaaaa aatctggcat gcatatccct cttattgccg
      361 gtcgcgatga ctttcctgtg taaacgttac caattgttta agaagtatat acgctacgag
      421 gtacttgata acttctgcgt agcatacatg aggttttgta taaaaatggc gggcgatatc
      481 aacgcagtgt cagaaatccg aaacagtctc gcctggcgat aaccgtcttg tcggcggttg
      541 cgctgacgtt gcgtcgtgat atcatcaggg cagaccggtt acatccccct aacaagctgt
      601 ttaaagagaa atactatcat gacggacaaa ttgacctccc ttcgtcagta caccaccgta
      661 gtggccgaca ctggggacat cgcggcaatg aagctgtatc aaccgcagga tgccacaacc
      721 aacccttctc tcattcttaa cgcagcgcag attccggaat accgtaagtt gattgatgat
      781 gctgtcgcct gggcgaaaca gcagagcaac gatcgcgcgc agcagatcgt ggacgcgacc
      841 gacaaactgg cagtaaatat tggtctggaa atcctgaaac tggttccggg tcgtatctca
      901 actgaagttg atgcgcgtct ttcctatgac accgaagcgt caattgcgaa agcaaaacgc
      961 ctgatcaaac tctacaacga tgctggtatt agcaacgatc gtattctgat caaactggct
     1021 tctacctggc agggtatccg tgctgcagaa cagctggaaa aagaaggcat caactgtaac
     1081 ctgaccctgc tgttctcctt cgctcaggct cgtgcttgtg cggaagcagg cgtgttcctg
     1141 atctcgccat ttgttggccg tattcttgac tggtacaaag cgaatactga taagaaagag
     1201 tacgctccgg cagaagatcc gggcgtggtt tctgtatctg aaatctacca gtactacaaa
     1261 gagcacggtt atgaaaccgt ggttatgggc gcaagcttcc gtaacatcgg cgaaattctg
     1321 gaactggcag gctgcgaccg tcttaccatc gcacctacac tgctgaaaga gctggctgag
     1381 agcgaagggg ctatcgaacg taaactgtct tacaccggcg aagtgaaagc gcgtccggcg
     1441 cgtatcactg agtccgagtt cctgtggcag cacaaccagg atccaatggc agtagataaa
     1501 ctggcggaag gtatccgtaa gtttgctatt gaccaggaaa aactggaaaa aatgatcggc
     1561 gatctgctgt aatcattctt agcgtgaccg ggaagtcggt cacgctacct cttctgaagc
     1621 ctgtctgtca ctcccttcgc agtgtatcat tctgtttaac gagactgttt aaacggaaaa
     1681 atcttgatga atactttacg tattggctta gtttccatct ctgatcgcgc atccagcggc
     1741 gtttatcagg ataaaggcat ccctgcgctg gaagaatggc tgacatcggt gctaaccacg
     1801 ccgtttgaac tggaaacccg cttaatcccc gatgagcagg cgatcatcga gcaaacgttg
     1861 tgtgagctag tggatgaaat gagttgccat ctggtgctca ccacgggcgg aactggcccg
     1921 gcgcgtcgtg acgtaacgcc cgatgcgacg ctggcagtag cggaccgcga gatgcctggc
     1981 tttggtgaac agatgcgcca gataagcctg cattttgtac caactgcgat cctttcgcgt
     2041 caggttggtg tgattcgcaa acaggcgctg atccttaact tacccggtca gccgaagtct
     2101 attaaagaga cgctggaagg tgtgaaggac gctgcgggta acgttgtggt acacggtatt
     2161 tttgccagcg taccgtactg cattcagttg ctggaagggc catacgttga aacggcacca
     2221 gaagtggttg ctgcattcag accgaagagt gcaagacgcg acgttagcga ataaaaaaat
     2281 ccccccgagc ggggggatct caaaacaatt agtgggattc accaatcggc agaacggtgc
     2341 gaccaaactg ctcgttcagt acttcaccca tcgccagata gattgcactg gcaccacaga
     2401 tcagaccaat ccagccggca aagtggatga ttgcggcgtt accggcaatg ttaccgatcg
     2461 ccagcagggc aaacagcaca gtcaggctaa agaaaacgaa ttgcagaacg cgtgcgcctt
     2521 tcagcgtgcc gaagaacata aacagcgtaa atacgcccca cagacccagg tagacaccaa
     2581 ggaactgtgc atttggcgca tcggtcagac ccagtttcgg catcagcaga atcgcaacca
     2641 gcgtcagcca gaaagaaccg taagaggtga atgcggttaa accgaaagtg ttgccttttt
     2701 tgtactccag cagaccagca aaaatttgcg cgatgccgct gtagaaaatg cccatggcaa
     2761 gaataatacc gtccagagcg aaataaccca cgttgtgcag gttaagcaga atggtggtca
     2821 tgccgaagcc catcaggccc agcggtgccg gattagccaa cttagtgttg cccataattc
     2881 ctcaaaaatc atcatcgaat gaatggtgaa ataatttccc tgaataactg tagtgttttc
     2941 agggcgcggc ataataatca gccagtgggg cagtgtctac gatcttttga ggggaaaatg
     3001 aaaattttcc ccggtttccg gtatcagacc tgagtggcgc taaccatccg gcgcaggcag
     3061 gcgatttgca gtacggctgg aatcgtcacg cgataggcgc tgccgctgac cgctttaacc
     3121 ccatttagtg ccgcacctac agggcctccc agccccgcgc cgcgcagcaa accatgccca
     3181 agtacgctca ttgctgcgtg ggtgcgtaaa atgcgggtca gttggctgga aagcaaatgc
     3241 gacacgcctt ttgccaataa tttgtctttc atcagcagcg gcagcagctc ttccagctca
     3301 ttcaccctcg catcgaacgc gtgcagaaac tcctgcttat gttcctcgtc cattttcttc
     3361 caggtattac gcagaaattg ttccagtaac tgttgctcaa tttcaaacgt agacatctct
     3421 ttgtcggctt tcagcttcaa tcgttttgaa acatcgagca aaatggcccg atacaattta
     3481 ccgtgtccgc gcagtttgtt ggcgatacta tcgccaccaa aatgctgtaa ttctccagca
     3541 atcagctgcc agttgcggcg atgt
//
LOCUS       DAJTYD010000243         3563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTYD010000243 DAJTYD010000000
VERSION     DAJTYD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.247"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..285
                     /locus_tag="OO585_003799"
                     /pseudo
     CDS             1..285
                     /locus_tag="OO585_003799"
                     /inference="COORDINATES: protein motif:HMM:NF019996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(381..1118)
                     /locus_tag="OO585_003800"
     CDS             complement(381..1118)
                     /locus_tag="OO585_003800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631225.1"
                     /translation="MALPFAADACLVASSFEADADEADDDAELADDAAAVFEDSALVS
                     DAFAFVSDVFAAEADLAAALACSVASPAFVVAVEADDAALSADFPAAVALAEACPALV
                     DAALADDAAAVFEPAAAEALCPAAVSEDLAFVSDVFAAFAEFPAAVAEEAALLALEDA
                     FVSDDFAASFEAAASRAEVAASDAFVVAVDAEVAADCCDAAAFVSDVFAALALVAAAL
                     FEDSAAAALFDASVAFVDAVPALEVAD"
     gene            1076..1288
                     /locus_tag="OO585_003801"
     CDS             1076..1288
                     /locus_tag="OO585_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631226.1"
                     /translation="MRRPDRRQQRRVAPRRHPRRRQRQLAVRRRQLRAKVRRNLQQRA
                     PRQRQNGQRILHPPWRLRMRARRKRG"
     gene            1237..1389
                     /locus_tag="OO585_003802"
                     /pseudo
     CDS             1237..1389
                     /locus_tag="OO585_003802"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1366..1545
                     /locus_tag="OO585_003803"
                     /pseudo
     CDS             1366..1545
                     /locus_tag="OO585_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2224..2896
                     /locus_tag="OO585_003804"
                     /pseudo
     CDS             2224..2896
                     /locus_tag="OO585_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            3069..3491
                     /locus_tag="OO585_003805"
     CDS             3069..3491
                     /locus_tag="OO585_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6631227.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVT
                     WLSPEGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIA
                     TEEETSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
BASE COUNT          954 a          932 c          989 g          688 t
ORIGIN      
        1 tctgaaaatc cggatgaagc cgggcgttac agcatggacg ttgagtacgg tcagtacagc
       61 gttattctgt tggtggaagg attcccgccg tcacatgccg ggaccatcac cgtgtatgaa
      121 gattcccgac ccggtacgct gaatgatttt ctcggtgcca tgacggagga tgatgcccgt
      181 ccggaggcac tgcgccgttt tgagctgatg gtggaagagg tggcgcgtaa cgcgtccgtg
      241 gtggcacaga acacggcagc cgcgaagaag tcagccagtg atgccagcac atcagcccgt
      301 gaggcggcaa cccatgcgac tgatgctgca ggctcagcac gcgcagccag cacgtcagcc
      361 ggacaggccg cgtcgtcggc tcagtcagcg acttccagcg caggaacggc atcaacaaag
      421 gccactgaag catcaaaaag tgctgccgct gcagagtcct caaaaagcgc ggcagctacc
      481 agtgccagtg ccgcgaaaac gtcagaaacg aatgctgcag cgtcacaaca atcagcagcc
      541 acttctgcat ccaccgcgac tacgaaagcg tcagaagctg ccacctcagc ccgggatgcg
      601 gcggcttcaa aagaggcggc aaaatcatca gaaacgaacg catcctcgag cgccagtagc
      661 gcagcttcct cggcaacagc ggcaggaaat tccgcgaagg cggcaaaaac gtcagagacg
      721 aacgccaggt cttctgaaac ggcagcggga cagagcgcct cggctgcggc aggctcaaaa
      781 acagcggctg cgtcgtctgc cagtgcagcg tcaacaagtg ccgggcaggc ctcagccagt
      841 gccaccgccg ccggaaaatc ggcagaaagc gccgcatcgt ctgcttcaac agccacaacg
      901 aaggctggcg aagccactga acaggccagc gcagcagcga ggtctgcttc cgcagcgaag
      961 acatccgaaa cgaacgcgaa agcgtcggaa accagcgcag aatcctcaaa aacggctgcc
     1021 gcatcgtcag ccagttcggc gtcgtcatcg gcctcatcgg cgtctgcttc aaaagatgag
     1081 gcgaccagac aggcgtcagc agcgaagggt agcgccacga cggcatccac gaaggcgaca
     1141 gaggcagctg gcagtgcgac ggcggcagct cagagcaaaa gtacggcgga atctgcagca
     1201 acgcgcgccg agacagcggc aaaacgggca gaggatattg catccgccgt ggcgcttgag
     1261 gatgcgagca cgacgaaaaa ggggatagta cagctcagca gtgcgaccaa cagcacttcc
     1321 gagtcactgg cggcaacgcc aaaagccgtt aaggccgcgt atgacctggc taacgggaaa
     1381 tacaccgcac aggatgcaac gacagcacag aaagggataa tccagctaag cagcgcgacc
     1441 aacagcacgt ctgaaacgct ggcggcaacg ccaaaggcag taaaagcagc caatgacaat
     1501 gctgagaaac gtctgcagaa agatcagaac ggtgcggata tccctggcaa agacaccttt
     1561 acgaaaaata ttggtgcctg ccgtgccttc ggtgggtcag taagcacaac aacaggaaac
     1621 tggacgactg cacagtttat cgagtggctg gattctcagg gagcatttaa ccatccatac
     1681 tggatgtgca agggttcctg gtcttatggc aataataaaa tcattactga tactggctgc
     1741 ggtaatattc atctcgccgg agctgtcatt gaagtaatgg ggataaagtc agcgatgacg
     1801 atccacatta ccacaccgac cacctccact ggtggtggaa caactaacgc ccagtttacc
     1861 tatattaatc acggaacaga ttattcacct ggctggcgaa gggactataa ctccagaaat
     1921 aagccaacgg catcagagat cggggcgtta ccgtcaggtg gaacagcagt atcatcagtt
     1981 aatctgtctt caaaaggtcg ggtaaccgcg ctgacagaca atacgcaggg ggcaacaggt
     2041 cttgagttat acgaggtgta taacaacgga tatccaacag cgtatggaaa tatcattcac
     2101 ctgaaaggga tgacagccgt tggcgaaggt gagttactca tcggctggag tggtacaagc
     2161 ggtgctcatg ctccggcatt tattcgttca cgacgggata cgaccgacgc aaactggtcg
     2221 ccgtgggcgc agctttacac ctcggctcat cctcctgcag agttttatcc agtcggtgca
     2281 ccaatcccgt ggccatcaga taccgttccg tctggttatg ccctgatgca ggggcagact
     2341 tttgacaaat ctgcttaccc gaaacttgca gccgcttatc cgtcaggcgt gatccctgat
     2401 atgcgtggct ggacgattaa gggcaagccc gccagtgatc gagccgtatt gtctcaggaa
     2461 caggacggca ttaaatcaca cacccacagc gccagcgtat ccagtacgga tttggggacg
     2521 aaaaccacat cgtcgtttga ttacggcact aaatccacga ataacactgg tgcgcatacc
     2581 catagtgtta gcggtacggc tgcttcagcc ggtgcacata cccattcgat gacatttgtt
     2641 tcaggtggtt ccagtggtgc tccgggaagt ggatcacctg attattctaa atacagtgtt
     2701 aacacttctt ctgcaggcgc tcatacgcac tctgtatcgg gtactgctgc aagcgcaggt
     2761 gcacacgcac atactgtcgg tattggtgct catacgcact ccgttgcgat tggttcacat
     2821 ggacatacca tcaccgttaa cgctgctggt aacgcggaaa acaccgtcaa aaacatcgca
     2881 tttaactata ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca
     2941 taaaaattta taatctgctg gccggaacta atgaatttat tggtgaaggt gatgcatata
     3001 ttccgcctca tacaggtctg ccagcaaaca gtactgatat tgcaccgcca gatattccgg
     3061 caggcttcgt ggctgttttc aacagtgatg aggcatcgtg gcatctcgtt gaagaccatc
     3121 ggggtaaaac ggtttatgac gtagcgtcag gggacgcgtt atttatttct gaactcggtc
     3181 cgttaccgga aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca
     3241 cagcctgggt gaaagatgca gaagcagaaa aactgttccg gatccgggag gcggaagaaa
     3301 caaaaaacag cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag
     3361 atctggaaat cgcaacggag gaagaaacct cattgctgga agcctggaaa aaatatcggg
     3421 tgttgctgaa ccgtgttgat acatcaactg cacctgatat tgagtggcct acgaaccctg
     3481 tcagggagta atcattggga ttatgccgca gcacgtctta agcaagaacg tgctgcggtt
     3541 ggatgctatt ttttccctga agc
//
LOCUS       DAJTYD010000244         3520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTYD010000244 DAJTYD010000000
VERSION     DAJTYD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.248"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            318..2090
                     /gene="aspS"
                     /locus_tag="OO585_003806"
     CDS             318..2090
                     /gene="aspS"
                     /locus_tag="OO585_003806"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6631228.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2208..2660
                     /gene="nudB"
                     /locus_tag="OO585_003807"
     CDS             2208..2660
                     /gene="nudB"
                     /locus_tag="OO585_003807"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6631229.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2689..3429
                     /locus_tag="OO585_003808"
     CDS             2689..3429
                     /locus_tag="OO585_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6631230.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3461..>3520
                     /locus_tag="OO585_003809"
     CDS             3461..>3520
                     /locus_tag="OO585_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6631231.1"
                     /translation="MMAIILGIDPGSRVTGYGVI"
BASE COUNT          872 a          859 c          992 g          797 t
ORIGIN      
        1 tcaagcatgc tgactcctga aaaaacaaaa tcgtgcctca caccttaatg cgattagccg
       61 atggcggaac gacaaatttt gttaattttt atgagttcgg gcaacacctt tatagcaaac
      121 tggcgtagag tctgatttca ggcccgatat aaagtggtaa cgaggcgttc cagcgcgggc
      181 taggctctta cggtgcggca gaatttccag tataatagcc gccttttttc attcagttgt
      241 gacatacagc taacgctgcg acttggtcac ctgcgcagca gggggcatcc gccggcagct
      301 aagttaaggg atatctcatg cgtacagaat attgtggaca gctccgtttg tcccacgtgg
      361 ggcagcaggt gactctgtgt ggttgggtca accgtcgtcg tgatcttggt agcctgatct
      421 tcatcgatat gcgcgaccgc gaaggtatcg tgcaggtatt tttcgatccg gatcgtgcgg
      481 acgcgttaaa gctggcctct gaactgcgta atgagttctg cattcaggtc acgggcaccg
      541 tacgtgcgcg tgacgaaaaa aatattaacc gcgatatggc gaccggcgaa atcgaagtgc
      601 tggcgtcctc gctgactatc atcaaccgcg cagatgttct gccgcttgac tctaaccacg
      661 tcaacaccga agaagcgcgt ctgaaatacc gctacctcga cctgcgtcgt ccggaaatgg
      721 ctcagcgcct gaaaacccgc gctaaaatca ccagcctggt gcgccgtttt atggatgacc
      781 acggcttcct cgacatcgaa actccgatgc tgaccaaagc cacgccggaa ggcgcgcgtg
      841 actacctggt gccttctcgt gtgcacaaag gtaaattcta cgcactgccg caatccccac
      901 agttgttcaa acagctgctg atgatgtccg gttttgaccg ttactatcag atcgttaaat
      961 gcttccgtga cgaagacctg cgcgctgatc gtcagcctga gtttactcag atcgatgtgg
     1021 aaacttcttt catgaccgcg ccgcaagtgc gtgaagtgat ggaagcgctg gtgcgtcatt
     1081 tgtggctgga agtgaagggc gtggatctgg gcgatttccc ggtaatgacc tttgcggaag
     1141 cagaacgccg ttatggttct gataaaccgg atctgcgtaa cccgatggaa ctgaccgacg
     1201 ttgccgatct gctgaaatct gttgagtttg cagtatttgc ggggccggcg aacgatccga
     1261 aaggccgtgt tgccgctcta cgtgttccgg gtggcgcatc gctgacccgt aagcagatcg
     1321 acgaatacgg taacttcgtt aaaatctacg gcgcgaaagg tctggcttac atcaaagtta
     1381 acgaacgcgc gaaaggtctg gaaggtatca acagcccggt agcgaagttc cttaatgcag
     1441 aaatcatcga agccatcctg gatcgtactg ccgcgcaaga tggcgatatg attttcttcg
     1501 gtgccgacaa caagaaaatt gttgccgacg cgatgggcgc actgcgcctg aaagtgggta
     1561 aagaccttgg tctgaccgac gaaagcaaat gggcaccgct gtgggttatt gacttcccga
     1621 tgtttgaaga cgacggtgaa ggcggcctga cggcaatgca ccatccgttc acctcaccga
     1681 aagacatgac tgctgcagaa ctgaaagctg caccggaaaa tgcggtggcg aacgcttacg
     1741 atatggtcat caatggttac gaagtgggcg gtggttcagt acgtatccat aatggtgata
     1801 tgcagcagac ggtgtttggt attctgggta tcaacgaaga ggaacagcgc gagaaattcg
     1861 gcttcctgct cgacgccctg aaatacggta ctccgccgca cgcaggtctg gcattcggtc
     1921 ttgaccgtct gaccatgctg ctgactggca ccgacaatat ccgtgacgtt atcgccttcc
     1981 cgaaaaccac ggcggcagcg tgtctgatga ctgaagcacc gagctttgct aacccgactg
     2041 cactggctga gctgagcatt caggttgtga agaaggcaga gaataactga tatgactcaa
     2101 atacacgaaa tcattcgcgt tgcatcaagg cggcaattga gtgaactccc atgagcatag
     2161 ataactatgt gaatgggatg agcgaaggca gtcaacgaag aggcagcgtg aaggataaag
     2221 tgtataagcg tcccgtttcg atcttagtgg tcatctatgc acaagatacg aaacgggtgc
     2281 tgatgttgca gcggcgtgac gatcccgatt tctggcagtc ggtaaccggc agcgtggaag
     2341 agggtgaaac cgcgccgcaa gctgccatgc gcgaagtaaa ggaagaggtc accattgatg
     2401 ttgtcgctga acaactgacc ttaattgaca gtcagcgcac ggtagagttt gaaatttttt
     2461 cacatttacg tcatcgctat gcgccgggcg tgacgcgtaa tacggaatca tggttctgtc
     2521 ttgcgcttcc gcacgagcgg cagatcgttt tcactgaaca tctggcttac aagtggcttg
     2581 atgcgcctgc tgcggcggcg ctcactaagt cctggagcaa ccggcaggcg attgaacagt
     2641 ttgtaattaa cgctgcctga acaggcaggg ctatctggag atatttttat ggcaggtcat
     2701 agtaaatggg ccaacaccag acatcgtaaa gctgcgcagg atgctaagcg cggtaaaatc
     2761 ttcactaaaa tcattcgtga gctggtaacc gcggctaagc tgggcggtgg cgatccggac
     2821 gctaacccgc gtctgcgtgc ggcggtggat aaagcactgt ctaacaacat gacccgtgac
     2881 acactgaacc gcgcaattgc acgtggtgtg ggcggtgatg atgatgcaaa catggaaacc
     2941 atcatctacg aaggttacgg tcctggcggc acggcaatca tgattgaatg tctgtctgac
     3001 aaccgcaacc gtaccgttgc tgaagtgcgt catgcattca gcaaatgtgg cggtaacctc
     3061 ggtacagatg gttccgtagc ctatctgttc agcaaaaaag gcgtgatctc cttcgagaaa
     3121 ggcgatgaag acaccatcat ggaagcagct ctggaagcag gtgctgaaga cgttgtgacc
     3181 tatgatgacg gcgcgattga tgtctacact gcatgggaag aaatgggtaa agtgcgcgac
     3241 gctctggaag cggcaggtct gaaagcagac agcgcggaag tttccatgat cccgtccacc
     3301 aaagctgata tggatgcaga aaccgcaccg aaactgatgc gtctgatcga tatgctggaa
     3361 gattgcgacg acgtgcagga agtttaccat aacggtgaaa tctctgatga gattgcagcg
     3421 actctctgat gaggcctgct aaacagcaaa acggagacgc gtgatggcta ttattctcgg
     3481 cattgacccg ggttcgcgcg tgaccggcta tggcgtcatc
//
LOCUS       DAJTYD010000245         3456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTYD010000245 DAJTYD010000000
VERSION     DAJTYD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.249"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            78..440
                     /gene="queD"
                     /locus_tag="OO585_003810"
     CDS             78..440
                     /gene="queD"
                     /locus_tag="OO585_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6631232.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            518..1786
                     /locus_tag="OO585_003811"
     CDS             518..1786
                     /locus_tag="OO585_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6631233.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1777..2037
                     /locus_tag="OO585_003812"
     CDS             1777..2037
                     /locus_tag="OO585_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6631234.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFSITYRFG"
     gene            2054..2629
                     /locus_tag="OO585_003813"
     CDS             2054..2629
                     /locus_tag="OO585_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6631235.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2777..>3456)
                     /locus_tag="OO585_003814"
     CDS             complement(2777..>3456)
                     /locus_tag="OO585_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6631236.1"
                     /translation="FPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHWGNA
                     LTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNVTRD
                     PLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISGHLL
                     APEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEALVT
                     NIHADCQ"
BASE COUNT          779 a          898 c          910 g          869 t
ORIGIN      
        1 aagtcgtttt agataatgcg aaaaaacagc ctttccggta ctctacggcg gttttatcgt
       61 cctgtagaga aattatgatg tccaccacgt tatttaaaga tttcaccttc gaagccgctc
      121 accgcttacc acacgtcccg gaagggcata aatgtggtcg cctgcacggg cattccttta
      181 tggtgcgact ggaaattacc ggggaagtcg atccgcatac gggctggatt atcgatttcg
      241 ctgaactaaa agcggcgttt aagccaactt atgagcgcct cgatcaccat tatctcaatg
      301 atattccagg tctggaaaac ccaactagcg aagttttagc aaaatggatt tgggatcagg
      361 ttaaacccgt tgtgccgctg ttaagtgcgg tgatggtaaa agaaacctgc accgctggtt
      421 gtatctatcg cggcgaatga taagagtgtg tcggcggtca atttccctta agtaacgcta
      481 tgttagggta ttgtgttcag gatatctggg gcatgacatg gaagacgact gcgacattat
      541 tattattggt gccggtattg caggcaccgc ttgcgcgtta cgctgcgcgc gagcgggttt
      601 atccgttttg ttactggaac gcgctgaaat ccccggcagc aaaaatcttt ccggcggacg
      661 gttatatacc catgcactcg cggaactcct ccctcagttt catctgaccg cgcctcttga
      721 acgacgcatc actcacgaaa gcctttccct gttaacgccc gatggcgtaa cgacgttttc
      781 cagcttacag cccggcggtg aatcctggag tgtattacgt gcacggttcg atccgtggct
      841 ggttgccgaa gccgaaaaag aaggtgtcga atgtatcccc ggagcgacgg tggatgcact
      901 gtatgaagaa aacggcagag tctgtggcgt tatttgtggt gacgatattc tccgcgcccg
      961 ttatgtggtg ctggcagaag gtgccaacag cgtcctggct gaacgtcacg ggttagtgac
     1021 tcgtcctgct ggcgaagcga tggcgttggg gatcaaagaa gtgctgtcgc tggaaacatc
     1081 cgctattgaa gaacgttttc atctggagaa taacgaaggc gcagcgttgc tgttcagcgg
     1141 cggaatctgt gatgacttac ccggcggcgc atttctttat actaatcaac aaacgctctc
     1201 gttagggatt gtttgcccgc tctcttccct tacgcaaagt cgtgttccgg caagcgagct
     1261 gctgactcgc tttaaagcgc atccggcagt gcgcccgctt atcaaaaaca cggaatcact
     1321 ggagtatggt gcgcatctgg tgccagaagg tggcttgcac agtatgccgg tgcaatacgc
     1381 cggtaacggc tggcagctgg tgggcgatgc gttgcgcagt tgcgtcaata ccggaatttc
     1441 cgtgcgcggc atgggtatgg cgctgactgg cgcgcaggcg gcacaaacgc tgataagcgc
     1501 ctgccagcac cgcgagccgc aaaatctgtt tgcgctttat catcacaacg tagagcgcag
     1561 cctgctatgg gatgttctac aacgttatca gcatgttccg gcgcttttgc aacgccctgg
     1621 ctggtatcgg gcgtggcctg cgttaatgca ggatatttcc cgcgatttat gggatcaggg
     1681 tgataaacct gttccaccgc tgcgccagtt attctggcgt catttacgtc gtcatggcct
     1741 gtggcatctg gcgggcgatg ttatcaggag tctgcgatgt ctgtagccca taatctctgg
     1801 cgcgttgctg atgcgccgca cattgttccg gctgactccg ttgagcgtca gatggtacaa
     1861 tggttgatta acgcctgtcc ggcaggtctt ttttcgctca caccggaagg tgacttacgc
     1921 gttgattatc acggctgcct ggagtgtggc acctgccgtt tgctgtgcga cgaatccaca
     1981 ctacaacagt ggcgctatcc gccttccgga ttcagcatca cctaccgctt tggataaata
     2041 ataaggataa tttatgcccc tcttacacct gctccgccag aatccggtga ttgctgccgt
     2101 taaagataat gccagcctgc aactggcaat cgactctgaa tgccagttta tttccgtgtt
     2161 gtacggcaat atctgcacca tcagtaatat cgttaaaaag attaagaacg ccgggaaata
     2221 tgcttttatt catgttgatt tgctggaagg cgcgtcaaat aaagaagtcg tgattcagtt
     2281 tttgaaactg gtgaccgagg cggacggcat tatcagtacc aaagcctcaa tgttgaaagc
     2341 ggcaagagcg gaaggttttt tctgtattca tcgcctgttt attgttgatt caatttcgtt
     2401 tcacaacatt gataagcaag ttgcgcaatc gaatccggat tgtattgaga tcctgccagg
     2461 ctgtatgccc aaagtgctgg gctgggtgac agagaaaatc cgccaaccgc tgattgccgg
     2521 tgggctggtg tgcgatgaag aagatgcgtg taatgcgatt aacgcgggtg tcgtggcgct
     2581 ttccaccacg aataccgggg tctggacgtt agcgaaaaaa ttactttgac gggataatcg
     2641 taaccaattg aatttggttt gatttgtagg ccgcacgcca catccgacat tcagcgcctg
     2701 atgcgacgct tgacgcgtct tatcaggcct acaagtcccc aggccagaac cgtaggcagg
     2761 atcatccggc aacacattat tggcaatctg cgtgaatgtt tgtcaccagt gcctccagca
     2821 ccactttcca gtcatcaacc acgccaacat ctgcctgtga aaataccgca gcgctggcgt
     2881 catgattaat cgccaccaca aacttgctat tgcgtacgcc cgccattaac gctgctgcgc
     2941 cggaagcgcc aacaacaata cacacctcag gtgccagcaa atgtccggaa ataccaatca
     3001 ctttttcagc atcaacgcca ccgttcatca ctcgcgcccg actgtagccc acttcggctc
     3061 ccagcttttc tgccagcata gcgatttcct ggttatctgc ctctccgccc tgccccacca
     3121 ccagcacccg tctggcctca gctaatgggt cgcgagtaac atttttcaga tcctctgtgc
     3181 taaccagcca gtcgggaagc gcacccggga caatgttcag ttgttgtatt ccagagggca
     3241 acgtggcgtt tttcgctgct cccgcctgac gcgccagaga aaggcatagc gggcgttttt
     3301 ctgtttgcag cgtcgccgtt agcgcatttc cccagtggga tttgcgcacg cttacggttg
     3361 gaatatctaa cgaagtcacc tggcagatgc ttgcaccatg taaacgccag gccagccggg
     3421 tcgacagttc atcgccaaac gttcccggcg gaaaga
//
LOCUS       DAJTYD010000246         3414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTYD010000246 DAJTYD010000000
VERSION     DAJTYD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.250"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..139
                     /locus_tag="OO585_003815"
     CDS             <1..139
                     /locus_tag="OO585_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631237.1"
                     /translation="LLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPATPA
                     D"
     gene            complement(136..798)
                     /gene="exoX"
                     /locus_tag="OO585_003816"
     CDS             complement(136..798)
                     /gene="exoX"
                     /locus_tag="OO585_003816"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6631238.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(822..1478)
                     /locus_tag="OO585_003817"
     CDS             complement(822..1478)
                     /locus_tag="OO585_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6631239.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(1580..1810)
                     /gene="holE"
                     /locus_tag="OO585_003818"
     CDS             complement(1580..1810)
                     /gene="holE"
                     /locus_tag="OO585_003818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6631240.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            1949..2323
                     /gene="yobA"
                     /locus_tag="OO585_003819"
     CDS             1949..2323
                     /gene="yobA"
                     /locus_tag="OO585_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6631241.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2327..3199
                     /gene="copD"
                     /locus_tag="OO585_003820"
                     /pseudo
     CDS             2327..3199
                     /gene="copD"
                     /locus_tag="OO585_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            3212..>3414
                     /locus_tag="OO585_003821"
     CDS             3212..>3414
                     /locus_tag="OO585_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HCR6631242.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQCPL"
BASE COUNT          830 a          829 c          894 g          861 t
ORIGIN      
        1 tttattgctc tgtaccgaca tggactcagg ccatggcggt aaatctggcc gctttaaatc
       61 gtacgaaggc gtagcgatgg aatatgcttt tctggtcgcg ctggcgcagg gaacattacc
      121 cgctacgcct gcggattaag tattttccag ataatgtttc agtgttaaac gcagctccgg
      181 gctcatgctg tccaggttat taaatgacca gcgcagatag cccggatcgc gttcggcaac
      241 gtcggaaacc gctttgccac ggtatttgcc aaaggtgaag gtcgtcatca acgacggacg
      301 tccggtgata tcggccatct gttctgccgt ccagccggag gtgttcataa tatcgataag
      361 caacgcggcg gtgatataac agtcatacaa cgcgcggtga tgatgcaggc ccggcggcgt
      421 ctgtacattg agcttccgtg ttttatataa cgccatattg ctgtacttga tccctggcca
      481 caaacgacgg gccagtttca tagtgcaaat ccactcaccg ggcatctcag gcagtacgcg
      541 gcggtcaaag ctggcgttgt gcgcgacata ccattcacta ccgtaatagt gtgggatcac
      601 atcttcaatc cacggtttat cggcgaccat ggcttcggtg atgcgatgaa tcgccatcgc
      661 ttgtggacta ataggacgat cggggcgcac caggtggctc atggggttga cgatttttcc
      721 gtcaatgaca tcaacagagg caatctcaac gatccctccc tgcaaaccgc aggtttctgt
      781 atcgataatg cgcaacatgg catgctccag gccgaaaaag cctagcgtaa tggaatgata
      841 tcgccttgcc aaccctgtga tgaacgctga ccgactaaca ataatgaacc acgatcggcg
      901 cgaacgatga gacgaccata ttcatcccat agtgcactgt taccacgggc atttgccatc
      961 agcacggcaa tcgagtactg atgtgaaaaa aattgtaagc gacgggccga cgccagcagg
     1021 tcgggttctc ccagacattg ccctgtagta aacagtgaac aggtcgggtc catatccata
     1081 ccttgcggtt gttcatcgac caccgtgatg gttctggaac gacggcccag acatgcacca
     1141 tgactctgat ggtagatccc aggcgttttt cgccacggtg cgaacaccgc aattccacga
     1201 ataaagcgat cgttatattc aacgggaagg ccggcaatga tggtcattcg ccaggtcgtt
     1261 gcggcataac aaagcgggtc taacagtgag agatcgggtg gggcaggaag ggcacgtcgc
     1321 gaataatcac acgccagtaa agaaagcgat ggaaaaacta acagctgaca ttgttgcctg
     1381 gcggcggccc tcacaaactc cagatgatga gctacctgtt cggtgagtga ggttttacga
     1441 ggctcatatt gcgctgctgc aactttccag aacgacataa tgacttcctt ttcaaaatga
     1501 ctgcaaatta tgggtgcggt ttagaaaata ctcataattt agcaggcgtt atgtaagaaa
     1561 gtgtaactct gtataagttt tatttaagtt tgggctcgta aggtaaacgt gacagtttga
     1621 ccgaagccaa acggtgggca ataagcctct cgcgaaacca gctgcgcaaa tgttcaggct
     1681 gttcacgttc aaccgcttca gcgatcaccg gcatattgta gcgttcttta aatgccaccc
     1741 cggccgccgc caaatcgaca ttcactttat ccatttctgt ttgatccagt ttagccagat
     1801 tcttcagcat tcttaatctc ctcatcattc gcgggcaaaa gttactaaga gtgagccagg
     1861 atggcaagcc ggaattgatg atcctcgact cagacattag acaaggttat gctctgtaaa
     1921 aaagttatta caaaaagtaa tagataatat ggcttcaact gcacgctccc ttcgctacgc
     1981 gctggcaatc ctgaccactt cgttggtgac accttccgtc tgggcacatg cgcatttaac
     2041 gcatcagtat cctgcggcaa acgcgcaagt gacagctgca ccgcaggcaa tcactttaaa
     2101 cttctcggaa ggtgttgaaa caggattcag tggtgcaaaa atcacggggc caaaaaacga
     2161 aaatattaaa acattaccgg cgaagcgaaa tgagcaggac caaaagcaac tgattgttcc
     2221 actggccgat tcgctgaaac ccggaaccta caccgttgac tggcatgttg tttcggtgga
     2281 cgggcataaa acgaaaggac actacacctt tagcgtgaaa taaatcatgc tggcgtttac
     2341 ctagatcgcg ctgcgattta tccatttcac ctcgctgatg ctggtttttg gcttcgcaat
     2401 gtacggtgcc tggctggccc ccttgacgat tcgtcgcttg ctggcgaagc gttttctgcg
     2461 tttacaacag cacgctgccg tctggagttt gatcagtgcc actgcaatgc tcgccgttca
     2521 gggcgggttg atgggaacgg gatggacaga tgtattttcg ccaaacattt ggcaggctgt
     2581 tttgcaaacg cagtttggtg gcgtctggtt atggcaaatt gttctcgccc tcgtaacgtt
     2641 gatcgtcgcc cttatgcaac cgcggaatat gccacgtttg ctgtttatgc tcactaccgc
     2701 gcaatttatt ctgctggcgg gagtggggca tgcgacgctg aatgaagggg taacggcgaa
     2761 aatccatcag actaatcaca cgattcacct gatttgtgcg gcggcgtggt ttgggggatt
     2821 actgccggtg ctctggtgta tgcaactcat caaaggccgt tggcgacacc aggctattca
     2881 ggcgctgatg cgtttttcat ggtgcgggca ttttgcggtg atcggcgtac tggcaagcgg
     2941 cgtgcttagt gcattgttga ttaccggatt tccccccacg cttacgactt actggggcca
     3001 actcttattg ctcaaagcca tcctggtgat gattatggtg gtcattgcgc tggctaatcg
     3061 ttatgttctt gtaccgcgca tgaggcagga tgaagatcgt gcggcaccct ggtttgtgtg
     3121 gatgactaaa ctcgaatggg cgataggtgc ggtagtgctg gtgatcatca gcctgcttgc
     3181 gactctcgaa cctttttgat ggattacgaa aatgatgaaa aaaagtattc tggcgtttct
     3241 gttactcacc agttctgcag cggcgctggc tgcaccgcag gtgattaccg tcagtcgttt
     3301 tgaagtgggt aaagacaaat gggcgtttaa tcgcgaagag gtgatgctga cttgccgacc
     3361 gggtaatgct ttgtatgtta ttaatccgag taccctcgtg cagtgtcctt taaa
//
LOCUS       DAJTYD010000247         3409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTYD010000247 DAJTYD010000000
VERSION     DAJTYD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.251"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..449
                     /gene="tauA"
                     /locus_tag="OO585_003822"
     CDS             <1..449
                     /gene="tauA"
                     /locus_tag="OO585_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="with TauB and TauC is responsible for taurine
                     uptake; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6631243.1"
                     /translation="AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEK
                     HPEVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLT
                     PQQQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            462..1229
                     /gene="tauB"
                     /locus_tag="OO585_003823"
     CDS             462..1229
                     /gene="tauB"
                     /locus_tag="OO585_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6631244.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1226..2035
                     /gene="tauC"
                     /locus_tag="OO585_003824"
     CDS             1226..2035
                     /gene="tauC"
                     /locus_tag="OO585_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6631245.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            2050..2901
                     /gene="tauD"
                     /locus_tag="OO585_003825"
     CDS             2050..2901
                     /gene="tauD"
                     /locus_tag="OO585_003825"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6631246.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(3007..>3409)
                     /locus_tag="OO585_003826"
     CDS             complement(3007..>3409)
                     /locus_tag="OO585_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6631247.1"
                     /translation="YSTKFASSFYGPFREAAGSALKGDRKSYQMNPMNRREAIRESLL
                     DEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEYAMIKFAALAGAIDEEKV
                     VLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT          734 a          886 c         1022 g          767 t
ORIGIN      
        1 gtgcttatgt ctgggcaccg gcggttaacg ccctggaaaa agacggcaag gtgttgaccg
       61 attctgaaca ggtcgggcag tggggtgcgc caacgctgga cgtctgggtg gtgcgcaaag
      121 attttgccga gaaacatcct gaggtcgtga aagcgttcgc taaaagcgcc atcgatgctc
      181 agcaaccgta cattgctaac ccagacgcgt ggctgaaaca gccggaaaac atcagcaaac
      241 tggcgcgttt aagcggcgtg ccggaaggtg acgttccggg gctggttaag gggaatacct
      301 atctgacgcc gcagcaacaa acggcagaac tgaccggacc ggtgaacaaa gtaatcatcg
      361 acaccgcgca gtttttgaaa gagcagggca aggtcccggc tgtagcgaat gattacagcc
      421 agtacgttac ctcgcgcttc gtgcaataaa aggaggcgca gatgctgcaa atctctcatc
      481 tctatgccga ttatggcggc aaaccggcac tggaagatat caacctgacg ctggaaagcg
      541 gcgagctact ggtggtgctg gggccgtccg gctgcggtaa aaccacgttg ctgaatctga
      601 ttgccggttt tgtgccttat cagcatggca gcattcaact ggcgggtaag cgtattgagg
      661 gaccgggagc agagcgcggg gtggtttttc agaatgaagg gctgctaccg tggcgcaatg
      721 tacaggacaa cgtggcgttc ggcctgcaat tggcaggtat agagaaaatg cagcgactgg
      781 aaatcgcgca ccaggtgctg aaaaaagtgg ggctggaagg cgcagaaaaa cgctatatct
      841 ggcagctttc cggtggtcaa cgtcagcggg tggggattgc ccgcgcgctg gcggcgaatc
      901 cccagctgtt attactcgac gaaccgtttg gtgcgctgga cgccttcacc cgcgaccaga
      961 tgcaaactct gctgctgaaa ctctggcagg agacgggcaa gcaggtgctg ttgattaccc
     1021 acgatataga agaagcggtg tttatggcga ctgaactggt tctgctttca tccggccctg
     1081 gccgtgtgct ggagcggctg ccgctcaact ttgctcgccg ctttgttgcg ggagagtcga
     1141 gccgcagcat caagtccgat ccacaattca tcgccatgcg cgaatatgtt ttaagccgcg
     1201 tatttgagca acgggaggcg ttctcatgag tgtgctcatt aatgaaaaac tgcattcgcg
     1261 gcggctgaaa tggcgctggc cgctctcgcg tcaggtgacc ttaagcattg gcacgttagc
     1321 ggttttactc actgtatggt ggacggtggc ggcgctgcaa ctgattagcc cgctgttttt
     1381 gccgccgccg caacaagtac tggcaaaact gctcaccatt gccggaccgc agggatttat
     1441 ggacgccacg ctgtggcagc atctggcagc cagcctgacg cgcattgtgc tggcgctact
     1501 cgcggcggtg gtgatcggta ttccggtcgg gatcgccatg gggctaagcc cgacggtgcg
     1561 cggcattctg gatccggtaa tcgaacttta tcgtccggtg tcgccgctgg cttatttgcc
     1621 gctgatggtg atctggtttg gtattggtga aacctcgaag atcttactga tctatttagc
     1681 gatttttgca ccggtggcga tgtcggcgct ggcgggggtg aaaagcgcgc agcaggttcg
     1741 cattcgtgcc gcccagtcgc tgggtgccag ccgtgcgcag gtgctgtggt ttgtcatttt
     1801 gcccggcgcg ctgccggaaa tcctcaccgg attacgcatt ggtctgggtg tgggctggtc
     1861 tacgctggta gcggcggagc tgattgccgc gacgcgcggt ttaggattta tggttcagtc
     1921 agcgggtgaa tttctcgcga ctgacgtggt gctggcggga atcgcggtga ttgcgattat
     1981 cgcctttctt ttagaactgg gtttgcgcgc gttacagcgc cgtctgacgc cctagcatgg
     2041 agaagtacaa tgagtgaacg tctgagcatt accccgctgg ggccgtatat cggcgcacaa
     2101 atttcgggtg ccgacctgac gcgcccgtta agcgataatc agtttgaaca gctttaccat
     2161 gcggtgctgc gccatcaggt ggtgtttctg cgcgatcaag ccattacgcc gcagcagcaa
     2221 cgcgcgctgg cccagcgttt tggcgaactg catattcacc ctgtttaccc tcatgccgaa
     2281 ggggttgacg agatcatcgt gctggatacc cataacgata atccgccaga taacgacaac
     2341 tggcataccg atgtgacatt tattgaaacg ccacccgcag gggcgattct ggcagctaaa
     2401 gaattacctt cgaccggcgg tgatacgctc tggaccagcg gtattacggc ctatgaggcg
     2461 ctctctgttc ccttccgcca gctgctgagc gggctgcgtg cggagcatga tttccgtaaa
     2521 tcgttcccgg aatacaaata ccgcaaaacc gaggaggaac atcaacgctg gcgcgaggcg
     2581 gtcgcgaaaa atcctccgtt gctacatccg gtggtgcgaa cgcatccggt gagcggtaaa
     2641 caggcgctgt ttgtgaatga aggctttact acgcgaattg tcgatgtcag cgagaaagag
     2701 agcgaagcct tgttaggctt tttattcgcc catatcacta aaccggagtt tcaggtgcgt
     2761 tggcgctggc aaccgaatga tattgcgatt tgggataacc gcgtgactca gcactatgcc
     2821 aatgccgatt acctgccaca gcggcggatt atgcatcggg tgacgattct tggggataaa
     2881 ccgttttatc gggcggggtg atacgagtgc ggacggttcc ctcgccccct tggggagagg
     2941 gttagggtga gggggcgttc accgtacttt caacaggtta actccccctt tctgagagga
     3001 aaaaaattaa cgcagaatct tcttctcagc caaatccagc gcaaagtagc tgaaaatcag
     3061 atccgcaccc gcacgcttaa ttgaacctaa gctttcgagc acgactttct cttcatctat
     3121 agcacccgcc agcgcggcga acttaatcat cgcgtactca ccgctcacct gatacgcgcc
     3181 aatcggcaat tcagtacgtt cacgcagctc acgcacgatg tcgaggtacg ctccagcagg
     3241 tttaaccatc aggcagtctg cgccctgggc ttcatccagc agtgactcac gaatcgcctc
     3301 gcgacggttc atagggttca tctgatagct tttgcggtcg ccttttaatg cgcttccggc
     3361 agcttcacgg aacgggccat aaaaggaaga ggcgaacttg gtcgaatac
//
LOCUS       DAJTYD010000248         3333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTYD010000248 DAJTYD010000000
VERSION     DAJTYD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.253"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..2848
                     /locus_tag="OO585_003827"
     CDS             <1..2848
                     /locus_tag="OO585_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6631248.1"
                     /translation="QCYPNTAIVGMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEK
                     RTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQT
                     VPDGFGGTEPRMTFNAYLAQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVW
                     PYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYG
                     RNLLKMDAFGCTCRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDND
                     YAGTMTGGRVLSIDAATRTLTLDREVTLPETGTSAVNLINGSGKPVSVDITAHPAPDR
                     IQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDN
                     GARFEPQSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGS
                     GEDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPAT
                     IELTLGYFQITAVPRLAVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGP
                     HIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWELI
                     DNSQLADEMAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYML
                     KVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQL
                     FMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADISGNVNANSGTLNNVTINEN
                     CRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAV
                     AFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEF
                     SVSAWLVNNWYPTASISDLLVVVMKKATAGISIS"
     gene            2916..>3333
                     /locus_tag="OO585_003828"
     CDS             2916..>3333
                     /locus_tag="OO585_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6631249.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKG"
BASE COUNT          767 a          856 c         1024 g          686 t
ORIGIN      
        1 acagtgctac ccgaacacgg ccattgtggg gatgcaggtg gatgcggagc agtttggtgg
       61 tcagcagatg acggtgaact accatatccg cggtcgcatc atccaggtgc cgtcaaacta
      121 tgacccggaa aaacgcacgt acagtggtat ctgggacggc agtctgaaac cggcatacag
      181 caacaacccg gcctggtgcc tgtgggacat gctgactcac ccgcgctacg gcatgggaaa
      241 atgtctgggg gcggcggatg tggacaagtg ggcgctgtat gccatcgggc agtactgcga
      301 ccagacggtc ccggatggtt tcggggggac cgagccgcgg atgaccttta atgcgtacct
      361 ggcacaacag cgtaaggcgt gggacgttct cagtgatttc tgctctgcga tgcgctgtat
      421 gccggtatgg aacggtcaga cgctgacgtt cgttcaggac cgcccgtcgg atgtggtgtg
      481 gccgtacacc aacagcgatg tggtggtgga tgataacggc gtgggattcc gctacagctt
      541 cagtgccctg aaggaccggc acacggcggt ggaggtgaat tacaccgacc cgcagaacgg
      601 ctggcagacc tccacggaac tggtggaaga cccggaagcc atactgcgct acggacgcaa
      661 cctgctgaag atggatgcgt ttggctgtac ctgccgcggt caggctcacc gtgcaggtct
      721 gtgggtgata aagaccggac tgctggaaac gcagacggtg gacttcacgc tcgggtcaca
      781 ggggctgcgt cacacaccgg gtgacattat tgaaatctgt gataacgact atgctgggac
      841 tatgaccggc ggacgtgtcc tgtccattga tgctgccacc cgcaccctga cgctggaccg
      901 tgaggttacc ctgccggaga caggtacatc ggcggtgaac ctgattaacg gcagtggtaa
      961 gccggtgagt gtggacatca ccgcacaccc cgcgccggac cggatacagg tcagtaccct
     1021 gcctgatggt gtggagacat acggggtgtg gggactctcc ctgccgtcac tgcgccgtcg
     1081 cctgttccgc tgtgtctccg tccgggaaaa cacggatggc acctttgcca tcacggcggt
     1141 gcagcacgta ccggaaaaag aagccatcgt ggataacggt gcccgctttg agccgcagtc
     1201 aggctccctg aacagcgtca tcccaccggc agtgcagcac ctgacggtgg aggtgagcgc
     1261 agctgacggc cagtatctgg cacaggcgaa atgggacacg ccgcgggtgg tgaagggggt
     1321 gcgcttcagt ctgcgactga ccagcggaag cggagaagac agccgtctgg tgaccaccgc
     1381 tatcactgcg gatacagagc atcgtttcag tggtctgccg ctcggggaat acaccctgac
     1441 agtcagggca attaacagtt atggccagca gggcgaaccg gccaccacca ccttccggat
     1501 taacgcgcca gcaaaacccg ccaccattga actgacgctg gggtattttc agataacagc
     1561 ggtaccgcgt cttgcggtgt atgacccgac ggtacagttt gagttctggt tttcggaggc
     1621 aaaaatcgca gacacatctc aggtggaaac ctctgcccgt tatctgggga ccggcagtca
     1681 gtggagtgta tccggcccgc acattaagcc cgggaaggat ttctggtttt acgtgcgcag
     1741 cgtcaacctg gtggggaaat ctgcttttgt ggaggccagt ggacgggcca gcaatgatgc
     1801 agaagggtat ctggggctgt ttcgggaaaa aataggaaaa ctgcatctgg ctcaggggct
     1861 gtgggagctg atagacaaca gccagcttgc ggatgagatg gcggagatga agaccaccat
     1921 caccgaaacc cgcaatgaaa tcacacagac ggtcagtaaa acgctggagg accagagcgc
     1981 caccatacag cagatacagc gcgtgcagaa ggacacaaat gatgaccttg ctgcacttta
     2041 catgctgaag gtacagaaaa caaaaaatgg cataccctat gttgccggta ttggagcggg
     2101 gattgaggat actgatggcc agcccctgag caacatactg ctgctggctg accgtattgc
     2161 gatgattaac ccggaggacg gcaacaccac gccgttattt gtggcgcagg ggaatcagtt
     2221 gttcatgaac gatgtgttcc tgaagcggct gtttgcggtg agtatcacct cgtccggcaa
     2281 tcccccgacg ttttccctga cgccggaggg caggctgacc gcaagaaatg ctgatatcag
     2341 cggtaacgtg aatgcgaact ccgggacgct caacaacgtc acgattaacg agaactgccg
     2401 ggttctggga aaactgtccg cgaaccagat tgaaggcgat ctcgttaaaa cagtgggtaa
     2461 ggcttttccg cgggactccc gggcaccgga gcggtggcca tcagggaccg ttaccgtcag
     2521 gatttatgac gatcagcctt ttgaccggca aattgttatt ccggcggtgg cattcagcgg
     2581 cgctaagcat gagagagagc atactgatat ttactcctca tgccgtctga tagtgcggaa
     2641 aaacggtgct gaaatttata accgtaccgc gctggataat acgctgattt acagttgcgt
     2701 tattgatatg cctgccggtc acggtcacat gacgctggag ttttcggtat cagcatggct
     2761 ggtgaataac tggtatccca cagcaagtat cagcgatttg ctggttgtgg tgatgaagaa
     2821 agccaccgca ggcatcagta tcagctgaat tttataaccc atatacgggc gccagaaatg
     2881 gcgccttttt tattgcagaa aagcgagagg taattatgcg taaagtttgt gcagccattt
     2941 tgtccgcagc catctgcctg tccgtatccg gtgcgcctgc atgggcgtct gaacatcagt
     3001 ccacactgag cgcggggtat cttcatgccc gtacgaacgc tcccggcagc gataatctga
     3061 acgggattaa cgtgaaatac cgttatgagt ttacggacgc gctggggctg attacgtcct
     3121 tcagttatgc caatgctgaa gatgagaaaa aaacgcacta cagcgatacc cgctggcatg
     3181 aggattccgt tcgtaaccgc tggttcagcg taatggcggg gccgtctgtg cgcgtgaatg
     3241 aatggttcag cgcgtatgcg atggcgggtg tggcttacag ccgtgtgtcg actttctccg
     3301 gggattatct ccgcgtaact gacaacaagg gga
//
LOCUS       DAJTYD010000249         3201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTYD010000249 DAJTYD010000000
VERSION     DAJTYD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.254"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..128
                     /locus_tag="OO585_003829"
     CDS             <1..128
                     /locus_tag="OO585_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HCR6631250.1"
                     /translation="VESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            136..1464
                     /locus_tag="OO585_003830"
     CDS             136..1464
                     /locus_tag="OO585_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6631251.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1511..2836)
                     /gene="rimO"
                     /locus_tag="OO585_003831"
     CDS             complement(1511..2836)
                     /gene="rimO"
                     /locus_tag="OO585_003831"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6631252.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3049..>3201
                     /gene="bssR"
                     /locus_tag="OO585_003832"
                     /gene_synonym="yliH"
     CDS             3049..>3201
                     /gene="bssR"
                     /locus_tag="OO585_003832"
                     /gene_synonym="yliH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="BssS; regulator of biofilm through signal
                     secretion; disruption of this gene in Escherichia coli led
                     to effects on biofilm formation, alteration in expression
                     of a number of genes and mutations in bssS led to defects
                     in indole transport and autoinducer-2 uptake and
                     processing; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6631253.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNR"
BASE COUNT          821 a          721 c          794 g          865 t
ORIGIN      
        1 gcgtagagag cgaagacgtt gcacgcgaat taattaaatt aggatgtgtt caggctcagg
       61 ggtatctgta ccagaaacct atgccattct ccgcctggga taaaagtgga aaattagtaa
      121 aagagtagtt tacgtatgtc cagaatcaat aagttcgtac ttacagtcag tctgctgatt
      181 tttatcatga tttcagcagt tgcctgcggg atctacactc aaatggtaaa ggaacgggtg
      241 tatagcctga aacagtccgt tattgatact gcttttgcgg tggcaaatat tgctgaatat
      301 cggcgtagcg tggcaattga tcttatcaac acgctaaatc ccacggagga acagctgttg
      361 gttggtttgc gcacagctta cgccgactcg gtttccccct cttatttgta cgatgtcggt
      421 ccttatctga tttccagtga cgaatgtatt caggtaaagg agttcgagaa aaattattgt
      481 gcagatatta tgcaggttgt gaagtatcga catgtcaaaa atacagggtt tatctctttt
      541 gacggtaaaa ccttcgtcta ttacctctat ccggtaactc acaatcgtag tctgatattt
      601 ttgcttggtc tggagcgttt ttctttactg tcaaaatcgc tggcgatgga cagcgagaac
      661 ctgatgttct ctctatttaa gaacggtaaa ccggtgaccg gtgatgaata taatgctaaa
      721 aacgccatct tcaccgtttc ggaagcgatg gagcacttcg cctatttgcc gaccggattg
      781 tacgtatttg cgtataaaaa agatgtttat ttgcgggtct gtacattgat tattttcttt
      841 gccgcattgg tggctgtgat atcgggtgcc agttgcctct atctggtacg cagagtgatt
      901 aatcgtggta ttgtggagaa agaagccatc attaataacc attttgaacg cgtactggat
      961 ggcgggcttt tcttttcggc tgccgatgtc aaaaaactct acagtatgta taactcggcg
     1021 ttcctggacg acctgaccaa agcaatgggc agaaaatcct ttgacgaaga tttaaaagcg
     1081 ctgccggaaa aaggcagtta tttgtgcctg tttgacgtcg ataaattcaa aaacattaac
     1141 gacaccttcg gtcatttgct gggcgatgaa gtgttgatga aagtggtgaa aatccttaaa
     1201 tcacagatcc cggtagataa aggtaaaatc taccgcttcg gcggtgacga atttgcggtg
     1261 atttatacgg gtggaacgct ggaagagttg ctatcgattc taaaagaaat cgttcatttc
     1321 caggtgggaa gcattaattt aagtaccagt atcggtgtag cgcattcaaa tgaatgtacc
     1381 accgtcgaac gcttgaaaat gctggcggat gagcggctgt ataagagtaa gaaaaacggc
     1441 agggctcaga ttagctggca gtaatcatta ttcgcaggcc ggacaaatga ttttgcccgg
     1501 cctgaattaa ttaaacccgg ctaccccaca aatcgtactc atcggcgtgc tcgactttca
     1561 cacgcaggat atcacccggc ttaacgttgg tttcaccatt gagataaacc gcgccgtcga
     1621 tttccggtgc atctgccatg ctgcgaccaa tcgcgccttc ttcgtccact tcgtcgataa
     1681 tcaccagaat ttcacggccc actttctctt gcaggcgctc ggcggaaatc tgctgctgca
     1741 gctgcatgaa acggttccag cgttcttctt taacttcttc cggaacctgg tcaggcaggg
     1801 cattggcgtc tgcaccttca accgggctgt atttaaagca gccaacgcga tccagacgcg
     1861 cttctttcag gaagtcgagt agcatctgga aatcttcttc tgtctcgcca gggaagccga
     1921 caataaaggt tgagcgtagg gtcagttccg ggcagatttc gcgccactgt ttgatgcgcg
     1981 ccagttggcg atctacagaa cccggacgct tcatcagttt gagaatgcgc gggctggcgt
     2041 gctgcaacgg aatgtccaga tacggcagga ttttgccttc tgccatcagt gggatgacgt
     2101 cgtccacatg cggataaggg taaacgtagt gcagacgtgt ccagatcccc agtttcgata
     2161 actgttcgca caggctgacc atgctggttt ttaccggctc gccgttgtgg aagccagtac
     2221 gatgtttaac atcaacgcca taggcggaag tatcctgcga gatcaccaga atctctttaa
     2281 cgcccgcatc taccagacgt ttcgcttcac ttaacacttc gccaatcgga cggctcacca
     2341 ggtcgccgcg catagacgga ataatgcaga aggtgcagcg gtgattacag ccttccgaaa
     2401 ttttcagata ggcataatga cgcggcgtca gtttcacacc ttgttctggc accaggctca
     2461 ggaatgggtt gtgtttcggt tttggcacgt agtgatgaac gtgctccaga acctgctcat
     2521 agctatgagg cccggtgatt tccagcactt tcgggtggac ttcgcggatc tgatcttctt
     2581 ttgcccccag acaaccggtc acaattacct tgccgttttc attcaacgct tcaccaatgg
     2641 cttccagtga ttcttgtacc gcgctgtcaa taaagccgca ggtgttgacg atcaccatgt
     2701 ccgcatcgtc atagctcggt accacgtcat aaccttcagt gcggagttcg gtgagaatac
     2761 gctctgaatc gacaaggttt ttcggacagc caagggaaac aaagccgatt ttcggctggg
     2821 gagttacttt gctcatagct taaaaaatat tcagttacag gaaaggtcag ggcagggatt
     2881 ctacagagtt ctggataaaa tttgtatcgc aatctcattc gctggcggag gcgaaggaaa
     2941 tgtaaatttt gttaattcgg cgtgaagaat tgatcctgga cagcattttg ctcaaaaaat
     3001 agccatacta tttaattgca acaaggctgg aaagaggagg atcgaagtat gttcgttgac
     3061 agacagcgaa tcgatctgct gaaccggttg atcgacgcac gcgttgacct cgctgcatac
     3121 gtgcaactga ggaaggcaaa aggatacatg tccgtcagcg aaagcaatca tctacgagat
     3181 aactttttta aactgaatcg c
//
LOCUS       DAJTYD010000250         3173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTYD010000250 DAJTYD010000000
VERSION     DAJTYD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.255"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            102..3005
                     /locus_tag="OO585_003833"
     rRNA            102..3005
                     /locus_tag="OO585_003833"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3173
                     /gene="rrf"
                     /locus_tag="OO585_003834"
     rRNA            3101..>3173
                     /gene="rrf"
                     /locus_tag="OO585_003834"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          848 a          695 c          979 g          651 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgaatcgta agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccat tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatattg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgctgatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaactaa ggctgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatatgtagg tgaagtccct cgcggatgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggtcagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgttgacccg taatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgactccttg agagtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaaggtg ttttggcgga tgagagaaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atg
//
LOCUS       DAJTYD010000251         3129 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTYD010000251 DAJTYD010000000
VERSION     DAJTYD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.256"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..393)
                     /locus_tag="OO585_003835"
     CDS             complement(<1..393)
                     /locus_tag="OO585_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6631254.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVA"
     gene            complement(433..1329)
                     /locus_tag="OO585_003836"
     CDS             complement(433..1329)
                     /locus_tag="OO585_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6631255.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            1502..2380
                     /gene="yihU"
                     /locus_tag="OO585_003837"
     CDS             1502..2380
                     /gene="yihU"
                     /locus_tag="OO585_003837"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6631256.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            2405..>3129
                     /locus_tag="OO585_003838"
     CDS             2405..>3129
                     /locus_tag="OO585_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6631257.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAV"
BASE COUNT          761 a          753 c          816 g          799 t
ORIGIN      
        1 tgccacacgt aggctgttag tgataatgcg taaatcacgc cggttgagta atgccctggc
       61 aacggcttct acggttgtac cgatggtgat aaagacggta cagcgttcgg gaaggtaatc
      121 ggcgactgcc tcagcgatcg cccgtttctc ggcggtgagc gaaagttccc gttgctcaaa
      181 agcggtattc atgacgctgg agacgcgccc ggcccctcca tgatgacggg tgatcaagcc
      241 ctgctcactg agttttcgga tatcccggcg cacggtttgc gttgaaacat ccagcagttg
      301 tgccagttct tcaatattca tataaccgcg ttctgcgatt agatgaacga gttggtcatg
      361 gcgtggatta cctgtcactt cgatgatact catgaatact cccttgcccc tcctcggtag
      421 atgtttacca ttttacgcat aaagtgacaa aaatgattcg gtttgctcac gattaggaat
      481 gccggcccga ccgccaggct gggtacattt catcgcggca accgcactgg caaaacgaat
      541 agcctctttt gttggcattt tttctgccaa cgctaccgcg agtgcgccgt gaaaaacatc
      601 gcctgctcca gtggtatcaa ccacattgac agaaaatgcc tcctgttgac acaaatgacc
      661 atcttcaatc cacaacgatc cttcagaacc cagcgtaaca tacactttac ctgctgtttg
      721 tgtcgttgcc tggaacaaac cttcttcagg agactgcagg ccagtcatgc gtttcagccc
      781 tggtgttgag aacacggcgt gatcggcaag cgcgacaagt ggagaaatat cctgcggtgt
      841 catatcggcg tctaaaagcg ttgtcacacc cgcaaggcgc gctagcgaga atgctttttc
      901 agtaccgcta tgccagcgaa catcggccag aatcagatta taacgagaga aatcgatcgc
      961 ttctaaccat tcggcatcag taccgagatc aggacttgga taattaacga taatccgttc
     1021 gccatgctga tcaacgagga tcgctgattg cgacgaacgg gcgttaggat atcggcggca
     1081 aaatgcggta ttgacgcccc aaccctcaag ttctgcaagt aaggtatttc cacaactgtc
     1141 atcgccgacg cgaccgatga aatcaacctc cacgccaagc tttgcgattg caacagcggc
     1201 tgttgctgcc gggccgccac caatctcaag ataatgattt gcctgatatt tcccgccgcc
     1261 ctccggcaga gtgggcaaag aataaattct gtcctgaact gtgataccca cgcatgcaat
     1321 tctggtcata tttgtcttgt cctgtttaat tttgtcgtct ttggtcaatt taaatgacgc
     1381 aaatgatcaa attagtgatg cgtgtcgtgt ttttgaacat aaatcacaaa tatgctcaaa
     1441 aacagacaaa aacgaatttt caattgtcat tattttgtca atgtcagaca ggagagtatt
     1501 catggcaacc attgcatttc tcgggctggg gcagatgggt agcccaatgg ctaacaatct
     1561 attgcaaaaa gggcattcat tgcaggtatt tgatgtcaat gcacaggccg tcgatgcgct
     1621 agttacgcag ggggccactg cggcacaaac ccaagcggaa gcagcagcag gcgctgaatt
     1681 tatcatcact atgcttccca atggtgacat tgttcgacag gttctgttgg gggaaaaagg
     1741 cgtctgtgag gctgtttcat ctgacgcgtt attgattgat atgtcgacta ttcatccctt
     1801 acagaccgat gcattaatac gggagttaca agaaaaaggc atcagtatga tggacgctcc
     1861 cgtgggcaga acgtccgtga atgcgattga tggcacattg ctgattttag cgggtggcac
     1921 tgatgagcag attgcacgtg cacgcccgat tcttatgtgc atgggtaatg agctggtcga
     1981 ggctggcggc ccaggaatgg ggatccgcgt gaagctaatt aacaactata tgagcattgc
     2041 gcttaatgcg ctgtcctccg aggccgctgt gttatgtgag tcactgggat taaatcttga
     2101 tgtcgcaatt aaggtgatga gtggtacggc agcagggaaa ggacatttta cgaccacctg
     2161 gcctggcaaa gtgctgaaag gcgatctttc acccgccttc atggtggatc tggcgttgaa
     2221 agatctgcgt attgcagttg acgtcgctcg ggagacgggg acaccgctca atatggggat
     2281 tgcagcagaa agttattacg ctgcagcaag tcaagacggc aaagggcggc aggactggag
     2341 tgccctgcta aatcaggttc gccagcaggc tggtctttaa ttttatctat taacaggagt
     2401 ttctatgtca tcccacttca ccccatttac gctgaaagat atctcccgcc caggtggtgg
     2461 atttgccatg ttagctgtcg atcagcgtga ggccatgcgc ttgatgtttg cggcagcagg
     2521 gcagccaaaa cccatcgccg acagtgtact caccgatttt aaagttgccg cgacccgaat
     2581 cctttctcct tatgcttctg ccgttctggc ggataaacaa ttttgtcttg agcaaatagt
     2641 cgagcagggc gctgttgcta attcttgcgg cctgattgtc gctgccgatc tttttatccc
     2701 aggtaatggc atcccggttg atagcgttga aatagatatg tccgtcgacc cgcataaagc
     2761 ccgtgaaatg ggggctaagg caatgaaatt acttgtactc tggcgtgaag atgaacccgc
     2821 agaggaacgt ctggcaatgg ttgataaatt tgttcgtcgc tgccgtagtg ccgggctggt
     2881 gagcattatt gaacctgtgg tgcgtccacc tcgtcgcggt tgggactttg atcgtgaaag
     2941 cgccattgtt gccgccgccg cagagcttgg tggaacagag gctgatcttt acaaagccga
     3001 gatgccatta gggggcaaag gcgatgaaaa aaccttactt gccgcctgtc agcaacttaa
     3061 cgatcagatg aagatgccgt gggtaattct ttcttctggc gttgatgccg atatttttgg
     3121 ccgtgcagt
//
LOCUS       DAJTYD010000252         3109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTYD010000252 DAJTYD010000000
VERSION     DAJTYD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.257"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..898)
                     /gene="nimT"
                     /locus_tag="OO585_003839"
     CDS             complement(<1..898)
                     /gene="nimT"
                     /locus_tag="OO585_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6631258.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPA"
     gene            995..1816
                     /gene="nimR"
                     /locus_tag="OO585_003840"
     CDS             995..1816
                     /gene="nimR"
                     /locus_tag="OO585_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6631259.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(1773..2219)
                     /locus_tag="OO585_003841"
     CDS             complement(1773..2219)
                     /locus_tag="OO585_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6631260.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            2388..2492
                     /locus_tag="OO585_003842"
     CDS             2388..2492
                     /locus_tag="OO585_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631261.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(2493..2996)
                     /gene="yeaK"
                     /locus_tag="OO585_003843"
     CDS             complement(2493..2996)
                     /gene="yeaK"
                     /locus_tag="OO585_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6631262.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
BASE COUNT          851 a          767 c          791 g          700 t
ORIGIN      
        1 gcgctggcat aaagcagagc ccaacagcgc ccactgcgca catcaaggca acgaacgctg
       61 caataccacg ctggtctttc acatgatgta agaaaagagg gatcagcaaa ccgggagctg
      121 ctgtggctag ttgcagcaaa ccatgcagtg aacccgcctg tgcttcgcta tagccgtgac
      181 tgatgaggat cgccggaagc cagccaataa tcacgtaata gaccagtgag ttgctcccaa
      241 gaaacaatgt gacctgccag gcaagcggcg aacgccagat accccgagta tgtaaggcgc
      301 gcgaggtact caaatttgca tgttgttggc ttcgccactg tggcagccat aaaaaaagag
      361 ccagcagagg aaaacacatc agcatgagca acgcgccttg ccagccaaaa ccgttcaaag
      421 ccagcggcac taccatagcc gatccaagtg ccgccgcagc tcccattgtc agggaatatg
      481 cgccggtaag tctggcgacg gaatgaggga aatcgcgttt aattaatcct ggcagtaaga
      541 cattgcctaa tgcaatccca ccgccaatga ccgctgtacc gccaaataat aagtaaggcg
      601 aagggagaga gcgaattgcg ataccagcac agatcaaaag taacgcggca aacaggctac
      661 gttccatacc aaaacgtcgc gctaccgggg cggccaaagg tgagattagc gcaaaggcca
      721 ataatggcag ggtggtcaat aagccggttt gcgctgtcgt cagcgagtaa gcggaacgaa
      781 tcgtatccag taacggtgct gcgccggtaa aggtgacgcg taatgttgtg gcaatcatca
      841 gaatgccagc gataaggaca atcctgtttt tgccgcttaa tgaagttgaa caggtcatat
      901 gcttctcatt gttacaaacg aggctgtacc ttaccgtttt tattgttatg ttgaatcaag
      961 ctaaaatgac aaattatcgc taaattcgga caaaatgatg cacaggttga atctcaatgg
     1021 ctatgaaccc gaccgacacc atgaagccgc ggtcgcattt tgtattcacg ccggaacgga
     1081 tgagttaacc agtcctgtac atcagcaccg caaaggacag ctgatactag cattacatgg
     1141 tgcaattacc tgtacggtgg aaaatgcttt gtggatggtg ccgccgcaat atgcggtttg
     1201 gataccgggc ggggtggaac atagcaatca agtgacagct aacgctgaac tctgttttct
     1261 gtttatcgaa cccagcgcag tgacaatgcc gacaacatgt tgcacattga aaatatcgcc
     1321 cttatgccgg gaattgattt taaccctggc aaacaggacg acgactcagc gtgctgagcc
     1381 gatgacccgc agattaatac aggtcctgtt tgatgaactc cctcagcagc cacaacaaca
     1441 gctgcacctg cccgtttctt ctcatcctaa aatccgcacg atggtggaga tgatggcgaa
     1501 agggcccgtc gagtgggggt cattagggca atgggctggc ttttttgcga tgagtgaacg
     1561 caatcttgcg cgactgatcg taaaagagac ggggttgagt tttcgccagt ggcgtcagca
     1621 gttacagctg attatggcac ttcagggatt agtgaaaggc gatactgtac agaaagttgc
     1681 tcatacgctg gggtatgatt caacaacggc ctttattact atgtttaaga aaggcctggg
     1741 gcaaacgcca ggccgatata ttgcgggatt aactactgtt tccccacaat cagcgaaacc
     1801 agacccgcgg caataagcgg gccgaccggt acgccgcgaa acagcgccac gcctaaaaca
     1861 gtgcctacca gcagcccggc gaccagttgc ggctggctgc ccattaaggt cacgccgcgc
     1921 ccacccagcc aggagacaat cacgcctact gcaattgcca ccagcgattt ccagttcaga
     1981 aacgagtgaa tcaaggttga gggcggtagc gtcccgctgg cgatgggggc cataacgcca
     2041 atcgtcagaa tgattatccc gatacttagc ccctgttttt caatccacgg gaaaaaggtg
     2101 cttaacggtg tgacgcggac aatgatcaac accagaattg aaacggcgac agtggtgtta
     2161 tgactgataa agcccagcgc agctaacccg agcaggatca gcaaagtgac atcaaacatc
     2221 gtggtttcct tgccaaaaaa taataagcct gcttacttta cgcttgctgc aggaaggaag
     2281 tagaaaatct ttacgttttt tgcataaaaa tcaggcgaac tgtcatcaaa ctgccatttg
     2341 tgcacgctaa caataacgtg aaaacagcta tctcccgggg gcatgctatg aacgatcaaa
     2401 tgttcgtcga gacactgatt atcacgtcat cgttttttgc cattgctgtt gtgctggttt
     2461 tgtccgttct gcttattgaa cggacaggct agttacgcag tgcggcggaa attgaccagt
     2521 tctggttgcg caatgcgcaa ataatccgtg gttttcaaaa taacggattt atccagcatc
     2581 cctgcattga aggcgatttc atcaaatcgt tcgaagagta gagggtcggc aaccagtttg
     2641 agttttggat ggaagctgaa aggggggatc gcgccgaaga cacagcctgt gagttcatcg
     2701 acttcagcag ggctggctaa cgaagcgcgt aatccgccta tatggctggc aagttggctc
     2761 agatcggcct gttgatcggc agcgaggatc gccagaacat gttgatttac gccattgcct
     2821 ttgactttac ataccagtgc cttagctccc tggcctaaag cggtgccacg aatttctgac
     2881 accgcttcac atttacctac cgcttcatgg gtaacgacgc ggaagttagc accttcttgt
     2941 gataataaag cgattaaccg ctgatgcgtc acgcttcctt tagccatttc agtcatttca
     3001 cgctcctgag attacaagca aacaaccaca gaaggttatc atgaacggct gtttttgttc
     3061 tgcttattga catataaacg ctcatcggaa gctttatacg catcatgta
//
LOCUS       DAJTYD010000253         3082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTYD010000253 DAJTYD010000000
VERSION     DAJTYD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.258"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..705)
                     /locus_tag="OO585_003844"
     CDS             complement(<1..705)
                     /locus_tag="OO585_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631263.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGI"
     gene            complement(702..2723)
                     /locus_tag="OO585_003845"
     CDS             complement(702..2723)
                     /locus_tag="OO585_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6631264.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(2707..2889)
                     /locus_tag="OO585_003846"
     CDS             complement(2707..2889)
                     /locus_tag="OO585_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6631265.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          711 a          901 c          740 g          730 t
ORIGIN      
        1 gatccccctg ttcgtggcac ttcgtctgtc agcgtctgac ggttgtgtca ccgacgccgt
       61 aactggctgc gttttaaccg atgcggcccc ttttgcttta tcgttgtccg tgggagacga
      121 cttatccgta cttcccactg acacctctcc tgtattcatt acggtctgaa ataccgcagt
      181 aaccgtcagt aaggtcggac cattattact tcgcattcgg tagtcatatt tcacaaggtc
      241 gcaggatgtc catgtcttgt ccggcgtctc aatatcgtag agtcctgccg tggtacgcat
      301 ccgttcaagc gtttccagca cattcgtccg cgaggtggtg gaaaaatttg tcaggttcgg
      361 gacggatccg gaaaaggccg tccacccctc tacaatgaaa gtcacatgca gttccggcga
      421 tctctggatt ttattaaagg tggtataagc cccctgctcc acggggacgg tggaaacaga
      481 agcctccgcg cccacctcaa cgacaacaaa agaatccggg gagaaaggtt tgccaccctt
      541 ctgatgggca cctgccggat cattccgtgc gtaataaata ccgaatgacg gtgccagtac
      601 actattaatg agtcccagga caccgccgcc acgaactgca ctcagtacgt tactttcact
      661 gagcgaaaag ttattcaggg aaatattatc gaaagaaaaa ctcatcctgt taccccgctg
      721 gaataaaccg acacaagcgc cgaattcgtg atacgccgac gtgcgtcatc ggtaataccc
      781 ttcacattgt ccgacgttgt ggtgacattc agtgtcccga tatgcgtggt ttccgttacg
      841 gtggactgcg atacaggcgc aggatggagc gaccgtatgg ccattgccgc acccggataa
      901 ggcaggttcg ccagcacccg cggaatatag ttgcgggtct cctccggagc agcagccagt
      961 cccttacgct gaacatttcc ctctccccag ttatatgccg ccagagcctt agccagatcg
     1021 ccatgaaaaa aacgtatcag gccaccaagt tttctcgcgg cggcatcagc ggctttttcg
     1081 ggatcaaagg catcgttccc cctcagacca aattcactgg ccgtcttcgg catgaactga
     1141 aacagtccca tcgcaccagc aggtgagacg gcaaactgat taccacggga ttcggtgatc
     1201 gcaacgctgc gcagcagtcc gggcggcagg ttatattttt cctccagttg agacagtttc
     1261 ggttgcagcc agcctaacag ggcttccccg gtcttcgtcg gtcgctggcg gttttgcatg
     1321 gcattaccga gtttttcctg cgtcgcacgc ataccctgta gccaggattc accgaaggct
     1381 gcttccctcc cggttgcctg agaagcctga gtatccagca tgccctgctg ccatactgtg
     1441 ggtgattgtg cccgggtgat gttgccaggc ttttctccgg catccagttt tgtctgatac
     1501 gtctccatct ctttcttatt gaaaaagaaa gtcccgtctg aagcccagaa aaaaccatgt
     1561 gaatccagcc agtccttatt ttccctgcca acaatggatg tcagtaatcc gtcaacaacc
     1621 gggtaaagcg ccgttatcgc aaaaagaagg cctccggggc cactgagggc ttttgtcagc
     1681 ccccgcaccc atgacgccac tttcagcccg atcagcgtaa taatgacatt ctgccagccc
     1741 cccagttctc cggcagcctt attcgccagg gaggccactg actcaacgtc attcagaaat
     1801 gtggtgataa acctgttcac ttcctccgga tgttgctgca tccagtcacc gagtgtctcc
     1861 agccagtggc tgagcttcag cgcgtacggc atcaacgcgg tgctaatcgt catcccgatg
     1921 ccggaccagg tctgatcgag ctcaacaagc tcttttcgta gcttacgggc cagcgctgta
     1981 cgccccggcg taacctgcga acgggaagta aattcgtcca catctttcag cgcatgaccg
     2041 gagccaagaa actgctgtcc ggcataactg aagcccagcg cgttgccgta ggcagtttgt
     2101 tcggactttg tcagtcgcgg aaaaataacc gccagcttgc gcatgatggc ttcagtgctg
     2161 tcagtattta aatcaacact aatgcccgcc ctggctgcaa cctgaaacaa atcctgtaac
     2221 acaggatcaa aggaccttcc ggctctgaag gccgcttttg catttgcaag ccgggaaaat
     2281 gccccagaca tttccccttc atcaacatta tttgcccgcg cagcccgtaa ccatccgtcc
     2341 agatttctgg ccttcatgcc aaaagcatcg gattccacgg acagacggtt cagatcacgg
     2401 gcaaaacctg ttaccaggtt cttcagcccc cccagcgtca gactgacacc cgcaagagcc
     2461 agaatctcgc tacggatccc tgaaaaaaaa cgggacgccc gtttcccggc atcttccatt
     2521 cgacgggccg ttttatcagc atcttttccg gttttttcca gtgattcact ggttttcttc
     2581 tgtccctgac tgaatgtgtc agcgacacct tccatcaccg tcgtgagccg gttaagggca
     2641 gcatgtactg cctgttcccc ggcagtaaag tccctgtcat caatcccgag ggacagaaca
     2701 agctcatcaa gtacctgcgc catttctgtt ctcctgtatc acctgttcgt tatgggcatc
     2761 cacatgaatt atttccagca gatcccataa gtcctgcaca ccaaggacag aatccagttc
     2821 agcttttgaa gccttaccgg aggagataac ggtcgcaatg gtacggggaa cattaacgta
     2881 atccaccacc ccgaacggtc tgtcggggtc gagataacgc ggggggatat ctagctgacg
     2941 acggtagcga aaaaatccac atgcagctta aagacctccg cacgcagatt aagacgggta
     3001 atgatttctt caatatcctc ttcaacaagc ggtctccgga tgctgcgttt tttcggatcg
     3061 ggaacaaact gcacacagtc ca
//
LOCUS       DAJTYD010000254         3075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTYD010000254 DAJTYD010000000
VERSION     DAJTYD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.259"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..356)
                     /locus_tag="OO585_003847"
     CDS             complement(<1..356)
                     /locus_tag="OO585_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR6631266.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAA"
     gene            complement(439..1663)
                     /gene="mdtG"
                     /locus_tag="OO585_003848"
                     /pseudo
     CDS             complement(439..1663)
                     /gene="mdtG"
                     /locus_tag="OO585_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(1835..2755)
                     /gene="lpxL"
                     /locus_tag="OO585_003849"
     CDS             complement(1835..2755)
                     /gene="lpxL"
                     /locus_tag="OO585_003849"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6631267.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
BASE COUNT          778 a          797 c          773 g          727 t
ORIGIN      
        1 tcagctgctg cgcgtccttc ctcggtatcc agcggtggct caagctgaaa ttccccctcg
       61 tcccattcat gtaatgtatt ctcttcctgc cactcctggc gtatctctat ctcatcatag
      121 tcgccatcaa aaacactttg cgcggcttca ccgctaagca taggtaaaca ttcaccttct
      181 tccccttcgt cggcaaaaaa ctcaacttgc cacatgatgt cgccgtcctg caaaacgtat
      241 ttttgggcat tgaactgttg cacattcgca tcttcggcgt cgatgccggg gttgtctgca
      301 agaaattctt cgcgtgcagc gtcaatggct tcttcaagcg ttgcgtacat ggtcatcacg
      361 attctccctt tgagttgatg aggtttcagg gaaaaggata gctgattctc cgcttttgca
      421 agtatgaaag gcgaaaaatc agttcgatac ctggggtatt cgacgacgac gtagactgtt
      481 ccatgaatag actgcgttga ataacactac gccagcggtg acgagaaata ccgctctgaa
      541 accgtagttc gctgaaatcg ctgctcccat caatggtccg gtaacgttgc caatatcacg
      601 aaacgattgg ttatagctga agatacgccc ggcgatctgg ttgctcgagt tgtaaaccaa
      661 caatgtctgt acggcgggga gtagtgcacc atcggcggca ccgagcaaaa aacgtaaaat
      721 cccaagttgc aatggcgtct gaacgtaaga cattgggatc aacagcagta cagaaaagat
      781 cagcgctgta atcaggatct tttcgggtcc gattcgatcg ccaagtttgc cgagtcgtgg
      841 tgcacttagc agagccgcca cgcctggcac cgaggcgatc atgccactga taaaggcgac
      901 gttactgacg ttacccgcca gttcgcggac atacagcgtc agaatggggg caattgagcc
      961 cgtcgccacc tggatgatta acgtagtgac aaacaggctg agtaccagtt tcgggttttt
     1021 aagtgatgtc accacttccc gcatgtgcag catctctttt ttgctgaccg gctggaattt
     1081 ttctctgatg caaaacaggg tgacgaaaaa gcagagtatg agcacactgg tggtaataaa
     1141 gaataccgga cgtaagccgt agctatcggc gagcaggccg ccagccattg ggccgagcaa
     1201 cgcaccacca acgccgcctg tggagagcgt acccagcgcc cagccgcttt tattacgcgg
     1261 tacttgtgtg gcgataagag cattagcgtt ggggacaaat ccgccaagta acccaagaag
     1321 cgcccgcagg atcaaaaact gccagatatt ttgtgccagc cccatcaaca ccatcacgat
     1381 gcccatgccg agggcagagc gtaatagcat gagttttcgg cctttacggt cggcgagtcc
     1441 accccaaaac ggtgaggcga tggccgaaaa taaaaatgta atgctgaaga caataccgga
     1501 ccatattcag ggcggagtga ccggtaacgc caagctgctc aacgtagagg ggtaagaagg
     1561 gcattaccag actgaaggcg gcaccggtaa gaaaacagcc tagccaggcg acgatcaggt
     1621 ttcgtttcca gtttataggg gtgtcatttt cacagggtga catagcaatc cgctgttggt
     1681 gcgccaggcg cggtgaacat aagaagaaaa gataagcaca ctaattatgc gcccgacttc
     1741 cagggggcgc aatccagaga gcttttatcg ctaaatcagg gggatttgct gtggtaatgc
     1801 cggatgccat tctgaagcat ccggcagggg agatttaata gcgtgaagga acgccttccg
     1861 ggcgtgtttt aaagcgacgg tgtaaccaca tatactgctc tggtgccatc atgatgcatt
     1921 tttcgaccac tttgttcatc cacgcggcgg tagtttcggc atcatccagt ggcggagaac
     1981 actctggcgg cagcataatc aattgatacc ctttgccatc tggcttacgg cgtggaacga
     2041 agggcaccag acatgcgccg gacatccgtg ccagcatcca ggttccggtc gtggtcgcag
     2101 cctgctcaac ggcaaataac gggacgaaaa cgcttgagcg cgggccgtaa tcatgatccg
     2161 gtgcgtacca gaccacttcg ccttttttca gggctttaat catgcctttt aaatctttgc
     2221 ggtcgagcat cgatttattt gagcgcaaac ggccccaggt ttgtagccag tcaatcagtg
     2281 gattatcgtt cgggcggtaa acgccaatcc ccggttcctg cataccaaac tgccgcgcac
     2341 ccagctccag tgtcagaaaa tggatgccaa ctaacaggat gccgcgtttt tgcgcctgca
     2401 cgtcacgaat gtgttccatg ccgatcactt ccgtccagcg cgcgattcgg cggtccggcc
     2461 agaaccacgc catgccggtt tccatcaggc ccatgccaac ggattcgaaa ttcttcacca
     2521 ccattttacg gcgttcttgt tcgctcattt ccgggaagca cagttccagg ttgcgatgca
     2581 caatttttgc gcgtcgtttc ataaaacgta acgccagttt tcctaatcca caaccgaggc
     2641 ggtagataac cgggtagggc aattgcacga ctaaccaaag tacgccaata cccaaccagg
     2701 ttaaccaata acgcggatga agcagtgcgg tggagaactt gggtagattc gtcatatcaa
     2761 tcctgttttt caacctattc gggcaattgt atgtattgtc gcattttttc gcccgcaacc
     2821 aaaatttgtg gctgaagact gggcgaaatt gccgcgcttg taaataacaa ataattttta
     2881 atgcgcaaat gtagcgtaaa atgtgtggat gttaattatc gataattgct atatcatgcc
     2941 gcggattttt actttcccat ctcgcaggaa ccgtacacca tgccagtgtt acacaaccgc
     3001 atttccaacg acgcgctaaa agccaaaatg ttggctgaga gcgaaccgcg aaccaccatt
     3061 tcgttttaca agtat
//
LOCUS       DAJTYD010000255         3061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTYD010000255 DAJTYD010000000
VERSION     DAJTYD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.260"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            577..1293
                     /locus_tag="OO585_003850"
     CDS             577..1293
                     /locus_tag="OO585_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631268.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="OO585_003851"
     CDS             1845..2249
                     /locus_tag="OO585_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631269.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2946
                     /locus_tag="OO585_003852"
                     /pseudo
     CDS             2312..2946
                     /locus_tag="OO585_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          853 a          633 c          696 g          879 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ctggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 ggcctgaggt gtactggcaa tagcggacac caccatttgt tctttttcta agcagtcatc
     3001 tgataatatt ttatcctgaa ggctgccggg gagatattcc ccagacgaga gtgacgacgc
     3061 t
//
LOCUS       DAJTYD010000256         3019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTYD010000256 DAJTYD010000000
VERSION     DAJTYD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.261"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(4..79)
                     /locus_tag="OO585_003853"
     tRNA            complement(4..79)
                     /locus_tag="OO585_003853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Val,seq:tac)
     gene            complement(126..201)
                     /locus_tag="OO585_003854"
     tRNA            complement(126..201)
                     /locus_tag="OO585_003854"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(166..168),aa:Val,seq:tac)
     gene            complement(246..321)
                     /locus_tag="OO585_003855"
     tRNA            complement(246..321)
                     /locus_tag="OO585_003855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(286..288),aa:Val,seq:tac)
     gene            581..1996
                     /gene="gltX"
                     /locus_tag="OO585_003856"
     CDS             581..1996
                     /gene="gltX"
                     /locus_tag="OO585_003856"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6631270.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            complement(2047..2439)
                     /locus_tag="OO585_003857"
     CDS             complement(2047..2439)
                     /locus_tag="OO585_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6631271.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2441..2800)
                     /locus_tag="OO585_003858"
     CDS             complement(2441..2800)
                     /locus_tag="OO585_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6631272.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
BASE COUNT          737 a          748 c          760 g          774 t
ORIGIN      
        1 aagtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg aggtgctctc
       61 ccagctgagc taatcaccca cttcggtact tcacattgtt acaagaaatt tagctggtga
      121 gaaagtggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      181 tcccagctga gctaatcacc cacttctcaa tttcttgcta cacggcggag actacataaa
      241 gtagttggtg ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc
      301 tcccagctga gctaatcacc ccccgctgtg tggagtcgca ttatagggag agttcaaaat
      361 gagtcaacgc attttctaaa gaaattgttc gttcgtcgta aatttaagca agatgatcgc
      421 aaaatagacc gtgttgcgca atttgtcaac gaaaacaata atgcgtaagg tagaaacccg
      481 aactacattg aggaatcagg cgggagtgat agaatatcgc ccactcaatt tttccaggat
      541 ttgccggttg tcggcatctt tctaaacgta aggccatttc atgaaaatca aaactcgctt
      601 cgcgccaagc ccaacaggct atctgcacgt tggcggcgcg cgtactgccc tttactcctg
      661 gctttttgca cgtaaccacg gcggtgagtt cgtgctgcgt attgaagaca ccgatcttga
      721 gcgttccacg ccggaagcta tcgaagccat tatggatggc atgaactggc tgagcctgga
      781 gtgggatgaa ggtccgtact accagaccaa acgttttgat cgctacaacg cggtgatcga
      841 tcagatgctg gaagagggca ctgcttataa atgctattgc tctaaagagc gcctggaagc
      901 gctgcgcgaa gagcaaatgg cgaaaggtga gaagccgcgt tatgacggtc gctgccgcca
      961 cagccatgag catcacgctg atgatgaacc gtgcgttgta cgttttgcta acccgcagga
     1021 aggttctgtt gtttttgacg atcagatccg tggtccgatc gagttcagca accaggaact
     1081 ggacgatctt attattcgcc gtaccgatgg ttccccaacc tataacttct gtgtggttgt
     1141 cgatgactgg gatatggaaa tcacccacgt tatccgtggc gaagaccata tcaacaacac
     1201 gccacgccag atcaacattc ttaaggccct gaaagcgccg gtgccggttt acgcgcacgt
     1261 ttctatgatc aacggcgatg acggtaaaaa actgtccaaa cgtcacgggg cggtcagcgt
     1321 aatgcagtat cgtgatgacg gttatttgcc agaagcactg ctgaactatc tggtgcgtct
     1381 gggctggtcc cacggcgatc aggaaatctt cactcgtgaa gagatgatca aatacttcac
     1441 cctgaatgcc gtcagcaaat ctgccagtgc gttcaacacc gacaagctgc tgtggctgaa
     1501 ccatcactac attaacgcgc tgccgccgga gtatgttgct actcacttac agtggcacat
     1561 tgagcaggaa aatatcgata cccgtaacgg cccgcagctg gctgatctgg tgaaactgct
     1621 gggcgaacgc tgcaagacgc tgaaagagat ggcacagagc tgccgttatt tctacgaaga
     1681 ttttgctgag ttcgatgccg acgccgcgaa aaaacatctg cgtccggtag cgcgtcagcc
     1741 gctggaagtg gttcgtgaca aactggccgc gattactgac tggaccgctg aaaacgttca
     1801 tcacgctatt caggcgacgg cggatgagct ggaagtgggt atgggtaaag ttggtatgcc
     1861 gctgcgtgtc gccgtaaccg gtgcggggca gtctccggca ctggatgtta ccgttcacgc
     1921 aattggtagg acccgcagta ttgagcgtat caacaaagcg ctggctttta ttgctgaacg
     1981 cgaaaaccag cagtaattag cgcataaaag ataaacggca ggagataata tcctgccgtt
     2041 ttttatttat gctgattcgt caatatcgag acgggcgagg aaattgcgaa ttccttcacg
     2101 tgacaaaaat tgcgcgactt tttcctgctc aatgtctgtc atattatcta tcgcgttgat
     2161 gatttgtcga tacgccgggg cgcgtactcc aggagcgtat tcagcaattc tctcttcagc
     2221 ttccatcaac attggtgtgt tatggaaggc tggcgtgttc tgaataaact cgcaaacttg
     2281 cgtatcgacc agaataagtc tggcacgacc gcctttcacg ccgggaaatt tttccgtttt
     2341 ccagcctttt tctctgatcc aacggttaac agtttgttta gccacaccga ggcattcagc
     2401 cagttcttcg gtggtcattt tattgcgtaa tcttttcata ttatttgcta tcgcgaatcc
     2461 ctaagcgttg taacaatccg ataatccctt cccgcagaag caaggatgta aactgttttt
     2521 gttcaactgg cgtcatctct ttcgccagtg tcaccagcaa tacttcaagc ggcgcatcac
     2581 cagaaagggc aggcgcttct ccctgacctt ctggacgttc agcattgcga atatattcac
     2641 gaacctgttc attgacgtga accagtcggg ctttgccacc ctggacgcca ggttttggtg
     2701 acgttgtcca gccttccttg cgtacccatt tattaatggt ctggcggcta tagccggtca
     2761 gtctggcaag ttcatctggt gtcatcctct ccttgaacat aacaattccc tgaatgtatg
     2821 tgggtctatt agtagttcat tttataacac cactttacag gaaaccgtga catgaataac
     2881 tactgaatgc gagcggtttt ccaaaattgt tcatttgcct gatttttcag cgattgaaac
     2941 tacacgttgc aatttgccgt tgacacattc gggcggaatt catatgatgc cgcccgtcaa
     3001 ctcgacaagc tttacgtga
//
LOCUS       DAJTYD010000257         2998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTYD010000257 DAJTYD010000000
VERSION     DAJTYD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.262"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..660)
                     /locus_tag="OO585_003859"
     CDS             complement(<1..660)
                     /locus_tag="OO585_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631273.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PL"
     gene            complement(663..1373)
                     /locus_tag="OO585_003860"
     CDS             complement(663..1373)
                     /locus_tag="OO585_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631274.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1389..2894)
                     /locus_tag="OO585_003861"
                     /pseudo
     CDS             complement(1389..2894)
                     /locus_tag="OO585_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          822 a          733 c          668 g          775 t
ORIGIN      
        1 tagcgggttc tcaatcgctt gccgcgctct ttctttcagt aaaggatgat tatttctagc
       61 ccaggcattg atttgggtgg taatgacgtt atcgatatcg tcctggttat ctctgatata
      121 atcctcacaa tatctgttca ataaacaggc aagagaaacg gtcgaccaaa agccgggaac
      181 tgaattaatg gcatccagca atggcgcaag agcgatatcg gcacggaaaa acatttccag
      241 catatactgt aattcgacag cttcatcgcc ggatacccaa tcagtcatat actggcaggc
      301 aaatcgctgt aaaattgccc gttcatccgc acgccagttt tctttgttgg caaggttgat
      361 acggcgcaga gaaaaaacta cgtgaatatt gtcaaaatgc ccctgtgcaa caaattccag
      421 taaacgcggc agaaaatagc ggacctcatc gctattttgc ggatcaggcc ctgggctact
      481 attccaggca ttgattaagg aatacgggat cgctcgtaac gatgtactcc gtaatgcctt
      541 ttgttcctgc tgactcaggc aatattcaca acaaaccaca aatttctgcg gcacccgata
      601 acgacgaaag gtgtgataaa tctgctcaat taatgcctgc atggcggggc agggttgcat
      661 agttaccccc tcgcccgctg atcgcgtgta aacggttgat agacaaaacg agcattcgcc
      721 gcgttcaggc gaataagggc gctacgcgaa agggaaaagc tggggcgaat gcaactattg
      781 cagtctcgac agctttcctc tgcgcaattt tccactgagt aatgacgcgt gtagagtttc
      841 agttcggcct gcttatcgcg aaatccgcgt aaactgttat tgttatgaaa tttccaggca
      901 tctttaaaga cctgaaagat atagacatcc tcaatccagg agtcacattt tttaagcggg
      961 cgctttgcta ttatcacccg ctcatccgga tttagcatcg ccgtaaattg tacggtgacg
     1021 tcatttgtcg tgttcaggtc acattgaaca tctggtacat cactgatata ggggtcaaat
     1081 ccaaaatcag caccacaggc acaaagacgg ctaatggtgg cggtatcgag ggaaaatccc
     1141 ggccatactt tgctggaggt taagccaata tttaatgtga catcatatcg aagcaatccc
     1201 tcaagaccgt cagcaccgac gaacttttcc ccttcatcca gggcctggtt aataagttgc
     1261 tgaacaatgc cttcatccca gtatgccgat ttgcagttgg caatcgtaat atccacgcct
     1321 gtgaagggat cagtaccggc aaggcttgca aataatgtat taactaattc catttttatt
     1381 atccagcttt aaggtagcgt cttatacatc gggacataac ctacatcttc caccaaactc
     1441 tgtccctgcg gcgttaaaaa ccactccaac aatttttgtg tctctgacgt cgtattttcc
     1501 cgcgttacca taaaggcatc gatgatatag ggatatttgc cgttgcgaat gttttcggca
     1561 gtcggtgcaa taccgttaat cgctagcagt tttatatttt tatcggcgtt catttgtgtt
     1621 gcgtaataac ggaaggtgta acctattgcg ttgtttgtat tacggtattc ggcaacgact
     1681 ttaatcattc cctccatcat acttgccact tctgtttcct gcggcgagat catgcggaca
     1741 tttttcatga cctgggattg catcaccgtc tggctaccag agtcttccgg gcgttgctag
     1801 gtctggatct cctgatcgtt accgccaaca gtacgccagt tggtaattgc accactgaag
     1861 atgtcacgca cctgttgttc agtcagggaa ttaaccggat tatccgcatt gacgatgaaa
     1921 acaaacgctt cacgggcaaa aggcgtgtac agcaaagtaa tgcccgattc ctcagcgcgt
     1981 tttttctgcc caccggaagg ttgcgccaca aaaataatat cagcatcacc tttaacaatc
     2041 ctgttatacg catcgggggt acgagagctc tccagatatt ccctggtgtg aaaatcttcc
     2101 ggtataacgc tcaaagcata aaatgcagag gcataaattg ggtacgccgc cgtagcacca
     2161 tcgagtcgcg gccagttttg cgtaaactga atttgcggtt taccacgcag tggtgtcagt
     2221 tgattattga gtttatcggg tcgccaggcc caggtatcaa tattttcgcg cacaccgacg
     2281 ccgggattga gctgtttata taaataaccc tgataaccag cgacaaaacc aagcatgaga
     2341 aggataaaca ggagggaatt acggcaacgt agcccggatg taccggttaa aggatgtctg
     2401 gttggccggc cgtaccccag cgaaaaactg atatgcgcgc caacgggtat aatgactatc
     2461 caccagtact cacctccgac tactgagact aacaaaacgc ccagccaggg gagcataaga
     2521 tcagccaacc aacccgatcc catctggtga aattgataat ctgccagatg cagcgcgatt
     2581 atccagccaa ccaggattaa taaaccgggt actaaaaaaa gacattgtcg ccagacggct
     2641 atttctcttt tttctggtgc actatcgcct gcccatactc gccccgagaa aaatagcatt
     2701 aatgcgccca gcgccagcat aaaaatgttg agcagaaggt aacttgttgg cgtgataagg
     2761 atattacaaa cttcaataat accggcaagt ccgacaccca gcatagcaac aaccgccgcc
     2821 aaaaattgcg tcagtatggg gatgccgaaa aaagtcatta ccagcgagga caaaatccat
     2881 tttctgtttt gcattattct ttccattctt tttgaatggt gaaattatac tccccgagtc
     2941 cccttgcccc ttctggacac ttttccgaaa tgatggcgga aaaaaacggg accctttg
//
LOCUS       DAJTYD010000258         2948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTYD010000258 DAJTYD010000000
VERSION     DAJTYD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.263"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1000)
                     /locus_tag="OO585_003862"
     CDS             complement(<1..1000)
                     /locus_tag="OO585_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6631275.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCRLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETN"
     gene            complement(1262..2852)
                     /locus_tag="OO585_003863"
                     /pseudo
     CDS             complement(1262..2852)
                     /locus_tag="OO585_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT          936 a          543 c          463 g         1006 t
ORIGIN      
        1 aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc attatgcgtt
       61 ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg ccggtttggt
      121 cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa ggctgaagac
      181 tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata ttagtgtcaa
      241 gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct atagtaagca
      301 accggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta gcaaaaagcg
      361 cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc ctggtacttg
      421 tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg gggtttataa
      481 ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc cagatgggtt
      541 tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata ttattaagta
      601 aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg taatttttac
      661 tattaattgt cacaataaac tgataattat tatccctacg tagaatatga gcacgcatgc
      721 ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa attttgtcat
      781 cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc acaacctcac
      841 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
      901 acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt gcgattgtcg
      961 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atattattca
     1021 ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt tcgatggaaa
     1081 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatttc
     1141 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct ctacaatcga
     1201 ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca ctcatggagt
     1261 tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
     1321 gagctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
     1381 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
     1441 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg tcatatacag
     1501 gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc atgtgttgtt
     1561 tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct ttcatctgat
     1621 taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt cttattattc
     1681 cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg agcgttttaa
     1741 ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca gtatagttac
     1801 cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt atacgactat
     1861 ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat ttatcaccaa
     1921 aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg gtaatcgcat
     1981 cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc gtatctaata
     2041 aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg tcttcgtgtt
     2101 ttaataattc acataataaa ctccacccct tttccctgat acctatatgt cctgaaacga
     2161 gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa gccgtgttgg
     2221 gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa aacacagaat
     2281 taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc caacgctagt
     2341 cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata gaaaggccat
     2401 taactttata aattatgtct gatttatctc cggagaaact aataaaataa taaccatcct
     2461 tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta ttgtctggta
     2521 taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat tcttgaatat
     2581 ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact atattcattt
     2641 accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc gttatctatt
     2701 tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt tttatcaacc
     2761 ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg gcttgaagag
     2821 atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt ataacttgtt
     2881 agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca aagtaataat
     2941 acttatat
//
LOCUS       DAJTYD010000259         2921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTYD010000259 DAJTYD010000000
VERSION     DAJTYD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.264"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..77)
                     /locus_tag="OO585_003864"
     CDS             complement(<1..77)
                     /locus_tag="OO585_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631276.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVI"
     gene            complement(88..786)
                     /locus_tag="OO585_003865"
     CDS             complement(88..786)
                     /locus_tag="OO585_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6631277.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(786..1127)
                     /locus_tag="OO585_003866"
     CDS             complement(786..1127)
                     /locus_tag="OO585_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631278.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(1120..>2921)
                     /locus_tag="OO585_003867"
     CDS             complement(1120..>2921)
                     /locus_tag="OO585_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6631279.1"
                     /translation="RLIDEQASRVKSLQEKIAGYQYVLANPGWTTGDGFMINHLTSVK
                     TVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLAGLEDRRVALIRQQAAEQNKVYQS
                     MLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALTKTERELALS
                     RLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGGKSET
                     EKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQELLRNAALID
                     QQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERLRERMRELQQIRDSFRQKDA
                     DLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAESDVQRADWQKGLQEGFSN
                     WVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKVLMNAAI
                     VNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAF
                     AKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQ
                     IGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          580 a          995 c          656 g          690 t
ORIGIN      
        1 cttatcacga agccgcacga ctccgcaggc gcacaccgcc gggcatgcgc cagaatcgct
       61 gattcagtct gtgtcataaa tcgggattta ctgcgaaagt ttattaatgg aaaggaaacc
      121 gccaaaattg ccgacattcc tgcgcagttc acacccgcgc atgcacttgc tgcatctgtc
      181 cttacggata tccgtggtgg gtttatcgaa ctcatccgcc acagccccgc ccgtgtaacc
      241 acactcatca gagcggtagg tccacataca ggtgttcgcc agcatgatac gaccgggaaa
      301 cagcgccccg tccgtctcgg tcggtgtagc cagcacaaac gaggccgtca tggctgtcag
      361 ctgcgacatc tgctccacca cccagcggtc actcagctcc tgctccgggt ccgcctccgg
      421 attgcccgca acgaaattca ccgcatccag aaaacgggca tacacccggc ggcggaccac
      481 cgtggccccc accagactct gcaggtcttc cgccatcccg gtgaccagac cgaacagatt
      541 ggacaccgtc agcgacggtc tggcactgct gccccggccg ttcatctcaa agccgctgcc
      601 gtcaatcggg tatgcctcat acttacgccc ctgccaggtg accggctccc ctttttcatt
      661 cagctcatta cagaaaaaat accgctcacc accctgcacc gtcaggtcaa tttcccagag
      721 taccacccgc ggtgactgct ctgatttaac cgactcgctc agactttctt cgtgaatatc
      781 ctgcatcagt tcaccacctg ctcaatcgta caactgaaat cactgtacct ggcgttatct
      841 gtgacgctcc actcccggca taccaccctc accgtccggt tatgtttcgg cggtcgccac
      901 aaaaaggcac ggtaaccacc atgccacgat aaaaactctt ccagccatcg ccgggtcgcc
      961 tcatccgtca cccggaacac cgcctgaaac gtcttcagtc gggcattaag tcccgtcggg
     1021 cggcgctgtt cataaccgtc accaaaccgt accctcgcca ccgacggttt ctcactcacc
     1081 tgcatccctt cacgcgggac cagatgcagc gtttttatct cagccactca gcattcctcc
     1141 gtcacgtcgc atggacagca tcaccgcctg cacccgctgg tcaatcagtt gcacaaggct
     1201 gcccgcagct tccggcccta tctggccatt agtcccgtca ttctgaatgg cgatatggta
     1261 gaccggggaa tacaccagac ctgcactgcc gttcatactg cccaccgctc gcacacccag
     1321 cgagccatcc gccgcccggg tcaggggcat aatggcttca ggaccggcct cccccatcag
     1381 tcccgcccct tttgcaaagg caaagtacgt gggcgtatcc acaatactgt tgctgtacgc
     1441 actcaggttt gccgaggtat acacaccgcc ttttgcattg gccaccgcac cgcccagcca
     1501 gctaccaata ctgccgataa atcctcccgc tccggacata ctgtttgccg ccgtcttgat
     1561 cccgttgaca atcgcggcat tcataagaac ttttgatatt tcctgcagca ctgatgaggc
     1621 ccagctgcgc cattccactt tatttccgtt cagcatctcc gtgatgttat tcaccatccc
     1681 tgagataccc tccgtcgcaa gctgtgctgc ctgtgaggcg taatcggacg cattatccac
     1741 ccagttactg aatccctcct gcagcccttt ctgccagtcc gcacgctgca catccgattc
     1801 ggcataaaag gcttcctgct ctttcagacg ttcactcaga tactgcgcgt tctgtgccag
     1861 agcctgtctg taaaaatcct cactgatatc cccggtctga tactgagact gaaggtccgc
     1921 atccttctgg cggaaactgt cgcggatctg ctgcaactcc cgcatgcgtt cacggagccg
     1981 ctctccctgc ccgtacccca gcagttccgc ttcatttgat gcacgagcag ccacattatc
     2041 attcttcagg gtctcttccc gggaccgcaa ctgctcccgg attttttgct ggtcaatcag
     2101 ggccgcattg cgcagcagtt cctgcttctg catctccgtc agggttttca gttcgccctg
     2161 cgcagtctgg tatttcagct tcgccagctc tgtattctgc cccgccagtg ccagttgctc
     2221 tttctgctgc ttcagcagcc gggaaaaact gtcttccgct ttttccgtct ctgattttcc
     2281 accccgggat ttgggtttat tcgcctcgtt attgcgccag gcttccaggg cattactgat
     2341 ataacgttgt ctcgcctcct gatacggatc acccacaaaa ccgaggtcat ccgccgcata
     2401 ccccagtcgg acacgctctt tttcttcccc tttcagtctg gacagggcca gctcacgctc
     2461 tgtttttgtc agggcactct gctgtttatc atccagagtg gcctgtggca gccgtaacgg
     2521 cacattcacc agtccctgcc gctgctgaag cagttcatta cccagcccca gcagacggtt
     2581 gaattccgta tgctgaccgt tcataaccag catggactgg tacaccttat tctgctctgc
     2641 cgcctgctga cgaattaacg ccacacgacg gtcttccagc ccggcaagca catcctgaat
     2701 ggactgcgct ttttcctgca tcagtgccag acgggactgc tcaacggcaa gctgctctgt
     2761 tgcctgagca agcccttccg ttacggtctt caccgaggtc agatggttta tcatgaatcc
     2821 gtcaccggtc gtccagcccg ggttcgccag aacatactga tatcctgcga ttttttcctg
     2881 cagggatttc acccgactag cctgttcatc aatcagccgg t
//
LOCUS       DAJTYD010000260         2911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTYD010000260 DAJTYD010000000
VERSION     DAJTYD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.265"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..142
                     /locus_tag="OO585_003868"
     CDS             <1..142
                     /locus_tag="OO585_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6631280.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            211..1305
                     /gene="mnmH"
                     /locus_tag="OO585_003869"
     CDS             211..1305
                     /gene="mnmH"
                     /locus_tag="OO585_003869"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6631281.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1421..1786
                     /locus_tag="OO585_003870"
                     /pseudo
     CDS             1421..1786
                     /locus_tag="OO585_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2348..2797)
                     /locus_tag="OO585_003871"
     CDS             complement(2348..2797)
                     /locus_tag="OO585_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631282.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
BASE COUNT          769 a          670 c          686 g          786 t
ORIGIN      
        1 gatgcgccct ccttcgccat tgagtctggc atggcgcaaa tttggcagcg gcaaagcggt
       61 agaagatatt gtgaccttgt ttacccagcg caggccggaa atcagcggat ttttagaaat
      121 tttcggcaac ccacgcagtt aaaaatcaag tttccttgcg cgttggcgcg taaaatagcg
      181 cgcaagctga tttgaggttc acttttaact atgcaagaga gacacacgga acaggactat
      241 cgtgccctgc tgattgctga tacgcccatt attgatgttc gcgcccctat cgagtttgag
      301 caaggcgcaa tgcccgccgc tatcaatctg ccgttaatga ataacgatga acgcgccgcc
      361 gttggcacct gctataaaca gcaagggtca gacgcggcgc tggcgctggg gcataaactg
      421 gtggcgggtg aaattcgtca gcagcgcata gacgcctggc gggcagcgtg cctgcaaaat
      481 ccgcaaggta ttctctgctg tgcccgtggc ggtcaacgct cacatattgt gcaaagctgg
      541 ttgcatgcag cggggattga ttatccgctg gtggaaggcg gttataaggc actgcgccag
      601 accgcgattc aggcgactat tgaactggca caaaaaccga tagtgctgat tggcggttgt
      661 accggcagcg gtaaaacgct gttagtgcag caacagccga acggtgtcga tctggaaggg
      721 ctggcgcgtc atcgcggttc ggcgtttggt cgcacgttac aaccacaact tagccaggcg
      781 agttttgaaa atctgctggc tgccgaaatg ctaaaaaccg acgcccgtca ggatttgcgc
      841 ctgtgggtgc tggaagatga aagccggatg atcggttcga atcacctgcc ggaatgtctg
      901 cgcgagcgaa tgactcaggc ggcgattgcg gtggtagaag atccgtttga gatccgtctt
      961 gaacgcctga acgaagagta tttcttgcgt atgcatcatg attttaccca cgcgtacggc
     1021 gacgaacagg gctggcagga atattgcgaa tacctgcatc acggactttc ggctattaag
     1081 cgtcggctgg ggttacagcg ctataacgaa ctcgctgccc agctggatac ggcgctgaca
     1141 acgcaactca ccaccggcag caccgacggt catctggcct ggctggtgcc gttacttaaa
     1201 gaatattacg acccaatgta tcgctatcag cttgagaaaa aagcggaaaa agttgtcttt
     1261 cgcggtgagt gggcggaagt ggcggaatgg gttaaggcgc ggtaatgagg gacgtatttg
     1321 tattagtcgc gacttgatct gacacatggc cttgaaaggt tgagagttac cggttttgat
     1381 atgagtgtcg aatccttatc caaaacaaga ggtaactctc atgcttcata ctgccaatcc
     1441 cgtcatcaaa cacaaagccg gtttgctcaa tctggctgaa gaactcagca acgtatcaaa
     1501 agcctgtaaa atcatgggcg tttcgcgtga tacgttttat cgttaccgcg aactggtcgc
     1561 tgaaggcggt gtggatgcgc agattaatcg tagtcgccgc gctcctaacc ttaagaaccg
     1621 taccgatgag gcaactgaac aggctgttgt tgattacgcc gtcgctttcc cgacacatgg
     1681 tcagcaccgg gccagcaacg agctgcgtaa acaggggggt tttatctccg acagtggtgt
     1741 ccgttctgtc tggttactcc ataaccttga gaatctcaaa agacgatact gaacaaattt
     1801 tatcaggtga cgttccgtaa aaagttgtat ggagttctcg atgcagtcgg cgcgactatc
     1861 agtacccccg ctgaaccctg gactatcggg ccgccgtact ggggcatgtc accctggcgt
     1921 gccgctggtt ttccgctaat ggtttatttc ctctttcttt cccggcaaga atttatttta
     1981 atccggaagg attatttatt ttcagatagg gactcacatt aaacacgtaa cattttaatt
     2041 aatgcatggc tgaatatgta ttacacaatg tcagatatcg taaaagacca gtgcaattct
     2101 atgtaaactc tctgactgtt cattttattt gttgtttcag ggtcggaact caccctcaat
     2161 aaacaactga atacgagtag atattcatcg tctgagctat atggctttac acaatagccg
     2221 acatgtcttt tctcagtcag gaattttact tttcctcact acgaagttga cgacttcgat
     2281 atgggataga ctcttaattc aagcaattac tctgcacaag catcaggcaa tccagctcca
     2341 atatttgtta taaaataatg cgtaacatta atttcaccaa caaaaaaagg tttacaccat
     2401 ccccttgccc tggtatctta ccatatttac accacacttt ttttgtatct ggatcattct
     2461 ttgatacgat tttcgccact tgttccctgg ccctcactgt caatgttttc ttaaacctac
     2521 ccccatcatc atttgttagg gaaaaataag aaaaaaaata atcatcttct acatttgtgt
     2581 atattgatat atccaccgct acgtgcgaaa aaaaatccgg gagccatgta aaaactccat
     2641 cattttctgg tattgccata tagtttatgt aatgtttact tacaagagat tcatctttgt
     2701 ataaataaag ataagtaatt acgcataaaa caacaatgat tataatagca aaaataaata
     2761 ttatcatctt tgatagatta cttgagatag ccagcatctt gtaaagcctt tatcgttttt
     2821 ttatgctctg gattaatata atcactacat ctatctgagc aatctgttgt tgatggacat
     2881 gtcaacccat ggtcatttac agccaagtct t
//
LOCUS       DAJTYD010000261         2845 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTYD010000261 DAJTYD010000000
VERSION     DAJTYD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.266"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            232..543
                     /locus_tag="OO585_003872"
     CDS             232..543
                     /locus_tag="OO585_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6631283.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="OO585_003873"
     CDS             543..830
                     /locus_tag="OO585_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6631284.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="OO585_003874"
     CDS             complement(1367..2554)
                     /locus_tag="OO585_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6631285.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..>2845)
                     /locus_tag="OO585_003875"
     CDS             complement(2554..>2845)
                     /locus_tag="OO585_003875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631286.1"
                     /translation="LHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFFVDAGNKKLV
                     FWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVHWPDGVAPPF"
BASE COUNT          736 a          767 c          664 g          678 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagc
//
LOCUS       DAJTYD010000262         2739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTYD010000262 DAJTYD010000000
VERSION     DAJTYD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.267"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..135
                     /locus_tag="OO585_003876"
                     /pseudo
     CDS             1..135
                     /locus_tag="OO585_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            385..1251
                     /gene="ospB"
                     /locus_tag="OO585_003877"
     CDS             385..1251
                     /gene="ospB"
                     /locus_tag="OO585_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6631287.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1581..2321
                     /gene="phoN2"
                     /locus_tag="OO585_003878"
     CDS             1581..2321
                     /gene="phoN2"
                     /locus_tag="OO585_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6631288.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          903 a          497 c          516 g          823 t
ORIGIN      
        1 gcggtaagag aagttcatca tacttggcgc agttacgcga tgtttctgct agaggtgatg
       61 ccgtaaagaa cggcagaaca ttaccggaac aagatagcgg tctacctgca ctggtatcag
      121 acccggggct tccccggatg atctccacgg tactttaggt actgtgtcta tattatgtag
      181 gtaggatcaa ttaactttgt agttgaaatt ctttcattga aataatgaat aaaaaagaca
      241 aaccgcatta aacaatgcca gaatataatt gcatttttgt tccgtttttt taaatcatag
      301 tatacaaaga agcttgcata ctatatgaaa atataacacc ctttactatg atgtttataa
      361 aacaatatat ggagtcatgt aggtatgaat ttagatggtg ttagaccata ctgtagaaca
      421 gtcaataaaa agaatgaaag catatcagat attgcatttg cacatataat aaaaagggta
      481 aaaaattcat catgtactca cccaaaagca gcattggttt ttttaggaga gaaaggtttt
      541 tgtgatagca atgatgttct atctattatg ggacaacaaa taccaagagt atttaagaac
      601 aagatgttat atgattatgt ttttaaaaat gaaaaaagta aaaatgattt tctaaaaatg
      661 gctgaatcat ggctaccaca gagtgaacca atagtaataa ataatgatga tgacgcattg
      721 aatgctgctg cttatttttc tgtaaaaaaa gcgaaaataa aaacagtaaa cgatactgat
      781 tttaaagagt ataataaggt ttatattctt gggcacggta gtcctggttc tcatcaatta
      841 ggccttggtt cggaacttat tgatgtacaa acaatcattt caagaatgaa agactgtggt
      901 attctaaatg tgaaagatat ccgttttact tcatgcggct ccgctgataa agtggctcct
      961 aaaaatttta acaatgcccc tgctgaaagt ctttcttgta tccttaactc tctgcctttt
     1021 tttaaggaaa aagaatcttt gctagagcag ataaaaaaac accttgaaaa cgatgagtca
     1081 ttgagtgatg gtctaaaaat atccggctat catggatatg gagttcacta tggtcaagag
     1141 ctttttccct actcacatta tcgttcaact tcaattcctg ctgatccgga gcatacagta
     1201 aaaagaagct ctcagaaaaa gacttttatt attaataaag aactggatta gtataaaatt
     1261 tttaacctat agttaattgt ttgcatttaa gatgaagtta tttttttctt tagtagaaaa
     1321 gtgcttccct atgagggaaa atcacaaatc atcataatac agagacaaaa cgatacgaac
     1381 aaatagataa aaaacatcat tcttttacta cattattttt cgtgaatatg aaaaataatg
     1441 ttattacttt aatataagac tattttttgt ttttccatca ctctgttcaa atttttccgc
     1501 atgacttgtg ttttttgtaa cacagctcgt tttttacagc tgaccaaaat catcaattaa
     1561 ttatgctaag gaaataaatt atgaaaacca aaaactttct tcttttttgt attgctacaa
     1621 atatgatttt tatcccctca gcaaatgctc tgaaggcaga aggttttctc actcaacaaa
     1681 cttcaccaga cagtttgtca atacttccgc cgcctccggc agaggattca gtagtatttc
     1741 aggctgacaa agctcattat gaattcggcc gctcgctccg ggatgctaat cgtgtacgtc
     1801 tcgctagcga agatgcatac tacgagaatt ttggtcttgc attttcagat gcttatggca
     1861 tggatatttc aagggaaaat accccaatct tatatcagtt gttaacacaa gtactacagg
     1921 atagccatga ttacgccgtg cgtaacgcca aagaatatta taaaagagtt cgtccattcg
     1981 ttatttataa agacgcaacc tgtacacctg ataaagatga gaacatggct atcactggct
     2041 cttatccctc tggtcatgca tcctttggtt gggcagtagc actgatactt gcggagatta
     2101 atcctcaacg taaagcggaa atacttcgac gtggatatga gtttggagaa agtcgggtca
     2161 tctgcggtgc gcattggcaa agcgatgtag aggctgggcg tttaatggga gcatcggttg
     2221 ttgcagtact tcataataca cctgaattta ccaaaagcct tagcgaagcc aaaaaagagt
     2281 ttgaagaatt aaatactcct accaatgaac tgaccccata aagctggaca gcctgtatca
     2341 ggctatggag ggcccataga caaatctacc ctatatgagc ataggaggag tctatgggca
     2401 caccacgttt tacccctgaa tttaagggat tactggaaag gctgggacat atccctccgg
     2461 cagaagcaga aaaagcttat tatgctgcca tcggaaacga tgatctggca acctgagttc
     2521 acagataaaa cattctctag gaaactcggg gcggttccgt tcaccacatg caatgtggtg
     2581 ttgcagggga acggtctgca ccatccccta tgtcgatcaa tataacagaa atgacaactt
     2641 cagattcagg gcacaaccta aatatatttt aggtcacctc tcaaatcgtt tgcctgatac
     2701 cgctccattt tttaataaaa aaatcaatca tttttgaat
//
LOCUS       DAJTYD010000263         2695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTYD010000263 DAJTYD010000000
VERSION     DAJTYD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.268"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            50..799
                     /locus_tag="OO585_003879"
     CDS             50..799
                     /locus_tag="OO585_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6631289.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARWILTEETIL"
     gene            975..1472
                     /gene="rayT"
                     /locus_tag="OO585_003880"
     CDS             975..1472
                     /gene="rayT"
                     /locus_tag="OO585_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6631290.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(1696..>2695)
                     /locus_tag="OO585_003881"
     CDS             complement(1696..>2695)
                     /locus_tag="OO585_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR6631291.1"
                     /translation="LGYKLVALVDKAQGNPLTQRIRGVRQVISDGNGVLLPEIRIREN
                     FRLKPSQYAIFINGIKADEADIPADKLMAQPSSETYGEIDGVLGNDPAYGMPVTWIQP
                     AQKAKVLNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQLHNRLSSMAPRLAED
                     LSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVTKDHILLAADVRLALRRS
                     ITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDSVPVDPNMLNQFQSTMPQ
                     VKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEVPDELELKIMGALM"
BASE COUNT          607 a          734 c          694 g          660 t
ORIGIN      
        1 ctccggtctt ttaaaattct caccttcatt cagccggttt atgtccttca tgtcttcgtt
       61 cttgctcggc agatttctgc atccaggcgt gttttcgtta tgcgttttgc tccctttact
      121 cgccagcgcc accacatcac atatctcttt cagctacgcc gcccgccagc ggatgcaaaa
      181 ccgtgcgcgt ttattaaaac agtaccaaac ccatctgaaa aaacaggcca gctacattgt
      241 ggaaggcaat gccgaaagca gaagggcgct acgccagcac aaccgggagc agataaaaca
      301 gcatccagaa tggtttcctg cgccgctcaa ggcaagtgac agacgctggc aggcgttggt
      361 agaaaacaat catttcctca gcagcaacca cctgcataat atcaccgaag tggcgattca
      421 ccgtctggag caacagcttg gcaagcctta cgtctggggc ggcacgcggc ccgatcaagg
      481 cttcgactgt agcgggttgg ttttttatgc ctacaacaag atccttgagg ctaagctccc
      541 gcgcacggct aatgagatgt atcactacca tcgggcaacc attgtggcaa ataacgacct
      601 gcgccgggga gatttgctgt ttttccatat ccacagccgt gaaattgccg accatatggg
      661 cgtgtatctg ggagacggtc agtttattga gtcgccgcgc acgggggaaa ccattcgggt
      721 aagtcggtta gctgaacctt tctggcagga tcattttttg ggcgcgcggt ggatattgac
      781 ggaagagacg attttgtagg acggataagg cgtttacgcc gcatccggcg gtggtgcgca
      841 gatgcctgat gcgacgctga cgcgtcttat catgcctaca aatctgtgcg tttttgcgag
      901 ccgctttcct gttattccat cacctcattg ccgacctttc cttttcttct taccgttgag
      961 aaaaaggagt cgccatgtct gaatatcgtc gttattacat caagggggga acctggtttt
     1021 tcacggtgaa tttacgcaat cgtcgaagcc aactcttgac cacccagtac cagatgctcc
     1081 gtaacgccat tattaaagtt aagcgagaca ggccttttga aatcaacgcc tgggtcgttt
     1141 tgccagagca tatgcattgc atatggacat tacctgaagg cgatgatgat ttttcctctc
     1201 gctggcggga aattaaaaag caatttaccc atgcttgtgg attgaaaaat atctggcaac
     1261 cacgtttttg ggagcacgcc atccgcaata ccaaagatta taggcatcat gttgattata
     1321 tttatataaa tccagtaaag catggttggg taaagcaagt gagtgattgg ccattctcaa
     1381 cgttccatcg cgatgtcgcg cgagggttat atcccatcga ttgggcgggg gacgtaacgg
     1441 atataaatgc tggcgagcgt atcattttat aattatgcgc agatgcctga tgcgacgctg
     1501 gcgcgtctta tcatgcctac aaacctgtgc cggatcggta ggccggataa ggcgtttatg
     1561 ccgcatccgg cggttatgcg cagatgcctg atgcgacgct ggcgcgtctt atcatgccta
     1621 caaacctgtg ctggatcggt aggccggata aggcgttcac gccgcatccg gcagttgtgc
     1681 accaatgctg gatacttaca tcaacgcccc cataatcttc aactccagct cgtccggcac
     1741 ttcgttatac gacagcacat gcagccccgg cgcaaacaac cttgcataac gcgccagcaa
     1801 agggcgcagc tgcggtggca ccagcagcac cgggtctttc cccgccgctt tcatctgctc
     1861 tttcacctgt ggcatcgtac tctggaactg gttgagcata ttcgggtcca ctggcacgct
     1921 gtcgagcatc actttaccgc cctgttgcgc ctgattcact aggttagtca gcagattttc
     1981 cagctcatta ttcagggtat acaccgtcag ctcctgcttg cgaacgaacg gatgggtaat
     2041 gctgcgccgc agcgccaggc gcacatcggc tgccagcaga atatgatctt tggtcaccgc
     2101 gctactggcg accagcacgg tggcgatggt gacgatatcg cgcagggaaa cgccttcggt
     2161 cagcagcgcc cggtacactt tcagcaactg gctgtaattg agcaccgcgc ttaaatcctc
     2221 cgccaggcgc ggtgccatcg acgacaaacg gttatgcaac tgcgtaatat cgtcatagtt
     2281 aaataaatca ggaatatagc tgcgcacaat cttatttaca tgggtagcaa tcacgctggc
     2341 gctgtcgatc acctgatacc ccatattcag caccttcgct ttctgcgcag gctggatcca
     2401 ggtcaccggc atcccgtacg ccgggtcgtt ccccagcacg ccgtctatct cgccgtaggt
     2461 ttcgctggag ggctgggcca tcagtttatc cgccggaata tccgcttcat cagccttaat
     2521 gccgttgatg aaaatagcgt actggctggg cttaaggcgg aagttttccc gaatgcggat
     2581 ctccggcagc agcacgccgt tgccgtcgga tatcacctgt cgcacgccgc gaatacgctg
     2641 ggtgagcggg ttgccctggg ctttatcgac cagcgccacc agcttgtagc cgagg
//
LOCUS       DAJTYD010000264         2661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTYD010000264 DAJTYD010000000
VERSION     DAJTYD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.269"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            29..865
                     /locus_tag="OO585_003882"
     CDS             29..865
                     /locus_tag="OO585_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631292.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFTLSSSNNST
                     KNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            834..1397
                     /locus_tag="OO585_003883"
     CDS             834..1397
                     /locus_tag="OO585_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6631293.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1426..1959)
                     /locus_tag="OO585_003884"
     CDS             complement(1426..1959)
                     /locus_tag="OO585_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6631294.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1962..2519)
                     /locus_tag="OO585_003885"
     CDS             complement(1962..2519)
                     /locus_tag="OO585_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631295.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          759 a          570 c          592 g          740 t
ORIGIN      
        1 ggaaacggta aacagggctg gtaacgccgt gcaaaaaaat ggcgatacct tgtccggtgg
       61 gcttactttt gaaaatgact caatccttgc ctggattcga aatactgact gggcgaagat
      121 tggatttaaa aatgatgccg atggtgacac tgattcatac atgtggtttg aaacaggcga
      181 caacggcaat gaatatttca aatggagaag ccgccagagc accacaacaa aagacctgat
      241 gacgctgaaa tgggatgcac taaatattct tgttaatgct gtcattaatg gcagtcttgg
      301 agttggttcg acgaatgcgt taggtggtag ctctattgtt cttggtgata atgacaccgg
      361 atttaaacag aatggagacg gtattctgga tgtttatgct aatagtcagc gtgtattccg
      421 ctttcagaat ggagtggcta ttgcttttaa aaatattcag gccggggatg gtaaaaaatt
      481 cacgctatcc agctccaaca actccacaaa gaatgcaacg tttaatttat ggggtgcttc
      541 aacccgtcca gtagttgcag agttaggcaa tgaggcagga tggcatttct atagccagcg
      601 aaatacagat aactcggtaa tattttctgt taacggtcag atacagccca gcaactgggg
      661 gaattttgat tcacgctatg taaaagatgt tcgcctgggt acgcgtgttg ttcaattgat
      721 ggcgcgtggt ggtcgttatg aaaaagccgg acacgcaatt accggattaa gaatcattgg
      781 tgaagtagat ggcgatgatg aagccatctt cagaccaata caaaaataca tcaatggcac
      841 atggtataac gtcgcacagg tgtaaattat gcagcatatt aaaaaatatt aagtccggaa
      901 atccaaaaac aaaagaacaa tatcagctaa caaagaattt tgatgttatc tggttatggt
      961 ccgaagacgg taaaaactgg tatgaggaag taaataactt tcaggacgac accataaaga
     1021 ttgtatacga cgaaaataat attattgttg ccataaccaa agatgcccca acgcttaatc
     1081 ccgaaggctt tagtgtcgtt gagattccag atataacagc caatcgtcgt gccgatgatt
     1141 cagggaagtg gatgtttaag gacggaactg tggttaagcg gatttataca gcagacgaac
     1201 agcaacaaca ggccgaatca caaaaggtcg cgttgctttc tgaagctgaa agcgttattc
     1261 agccactgga acgcgctgtc aggctgaata tggcgatgga tgaggaacgc acacgactgg
     1321 agtcatggga acgctacagc gttctggtca gccgtgtgga tacagcaaat cccgaatggc
     1381 cacaaaagcc tgagtaaaaa ttaaggccca atatcgggcc ttctctcatt ctggttgttc
     1441 gggaaacgtt actggcaggc tggaggtgtc tgtagattcg actttctgcg catagagcat
     1501 ccactcggtt aatttttgtt tattctcgtc ggaaatgatg cccagccgta gctgtgagtc
     1561 ccatagctgg gttttatccc tgacaagttg caacaggctt tgcttttcat tttccgcttg
     1621 ttgcctctgc tcttcctcgg tataagttcg ctttatcact acgccatctt tgaacaacca
     1681 tttccccgaa atatcagccc ggcgatttgc tgtaatatca ggtaattcaa cgacgcttgc
     1741 gccttccgga ttaattgctg aaacatcctt ttcaatacaa ataataacgc cgttatggta
     1801 atagaccatt ttcaaagtgt ctggctggaa attcttttgt tcctcatacc agtttttccc
     1861 atcatctgaa taaagccatt tgatgttaaa ttgctttgtt agctggtatt gctcttttgt
     1921 tttagggttg ccagcagtaa tgttttttaa gtgcatcatc gttaaatact ccccgcgtta
     1981 taccacgtcc cattaatgca atactgaatt ggccttgcct gtgttgtatc aattaattca
     2041 tcacggtttc cgttaactga acccgtaacg acataacctg acctgtcaga ccagccggga
     2101 ccattccatg tctgaacaga tgacagaccg ccaaggcgaa tacctgtaat aaaccttgag
     2161 ttacattctg cccgcgtata tgcaccaaca tctcccgctg aaggttttcg tgtcgtggta
     2221 tagaaatctg accaacctaa ttcaacccca taaccatcac gagccgacct gtaggaaatg
     2281 ccaccatttt tataattcac acggaattgt acggcaggac agctaccagc atttatatta
     2341 aagtgaagaa ttaatgcaga tgcaccacca atcagggcgt tatatgcacc actatcccaa
     2401 ttccatccaa cggatgtatc accattaaca gtattacctg tttgtcggag tgcaaatgca
     2461 ttgacatttt tggcactaat tgaaatatcg ctagaaccat caaaaccaac tccagcaatt
     2521 tttcgtgctg ttttcagctt tgttgcagta gcggcattgc cggacagttc cccagaaagg
     2581 ccaccgctga atgtttgtcg attggtccag gtattcgctg tactgagtaa cggtattttc
     2641 tccccgcttg tgccgagttc t
//
LOCUS       DAJTYD010000265         2583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTYD010000265 DAJTYD010000000
VERSION     DAJTYD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.270"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..115
                     /locus_tag="OO585_003886"
     CDS             <1..115
                     /locus_tag="OO585_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR6631296.1"
                     /translation="QWAAIGVLGSLLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            120..464
                     /locus_tag="OO585_003887"
     CDS             120..464
                     /locus_tag="OO585_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6631297.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            515..1048
                     /locus_tag="OO585_003888"
     CDS             515..1048
                     /locus_tag="OO585_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6631298.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1204..1386
                     /locus_tag="OO585_003889"
     CDS             1204..1386
                     /locus_tag="OO585_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631299.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            1713..1865
                     /locus_tag="OO585_003890"
     CDS             1713..1865
                     /locus_tag="OO585_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCR6631300.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            1951..2175
                     /locus_tag="OO585_003891"
     CDS             1951..2175
                     /locus_tag="OO585_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631301.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          653 a          571 c          731 g          628 t
ORIGIN      
        1 tcagtgggcg gcaataggcg tgctggggag tctgttgttt gggctgctga catatctgac
       61 taacctgtat ttcaaaatca gagaggaccg tcgtaaggcg gcgcggggag agtaaagcga
      121 tgaagaaaaa atacgaactg gttgttaaag ggataaataa ttacccgaat aagattactg
      181 ttactgtggc actggaaatt ggtgggtatc cgtcactgtt gttgccagat gtggcgatta
      241 gtcttgaccg tactgaagat gccacgctgg agttttacga agctgaggcg aaaaagcagg
      301 cgaagcagtt tttcatggat gttgctgccg ggttatgtga aggggatggt ccgttgccgg
      361 aaaagcgccc cgtaatttta gaggcgcagg atgtgttgat aacctacaga ggaaaactac
      421 cgggaataat tactggttct ctgaagactc caccgctggc ctgaagactt aacatatcca
      481 gggatttgaa atcgataaac cctgataaat atccatgaac accaaaatca aatacggcct
      541 gtcggctgcc gttctggcgc tgattgccgc aggtgcgcct gcgcctgaaa tcctcgacca
      601 gtttctggat gaaaaggaag gtaaccacac cacggcatac cgtgatggcg cgggtatctg
      661 gaccatctgc cgcggtgcca tcctggtgga tagtaaacct gtcgtcccgg gcatgaagtt
      721 gtcgaaggaa aaatgcgacc aggtcaacgc cattgagcgt gataaggcgc tggcatgggt
      781 ggagcgcaat attaaagtgc cgctgaccga accccagaaa gcgggtatcg cgtcattttg
      841 tccctataac attggccccg gtaagtgttt cccgtcgacg ttttacagac ggattaatgc
      901 tggtgaccgc aggggagcat gcgaagcgat tcgctggtgg attaaggacg gtggcagaga
      961 ctgccgtatc cgctcaaata attgctacgg tcaggtatcc cggcgagacc aggaaagtgc
     1021 gctggcgtgc tgggacatcg acagataagc agaatatttt gctgaaaata aggcatgagc
     1081 acgcggacgg ataacacgaa atcctgcgaa ctggcgaaac gtaagtgaat aaaagtaaaa
     1141 accccgtttg ttggcaccag gcggggtttt gtgtttctga ccttgagtaa ggcaagggag
     1201 aacatggcga agtataaacg aattctgttg aggttgacca tgaaaaacgg ccttgaactg
     1261 aaagcgcctg taactgatga catcagcaga gcactggctt ttgccattaa gtgggtggcg
     1321 gtcggtgttg ctgtgtcccc gatgctgtat gggctggcaa aactggtcat tgcgttgaaa
     1381 tcgtgaaggg aggattaagc atgtcagaca aactcataac gctggcgaag atcctctgtg
     1441 taattgtcgg catttcattt tcactaatgc tggttgctct ttttctttcc atggcctgga
     1501 tgatgttgtc ttcgtcgggg ctgctggggt gaacataaac cgaatgcttt ccgcgtttac
     1561 cgttattctg ctggtggtct gtggtgcgct gtggctggca acagaccatt accgtgataa
     1621 cgccatcacc tacaaagcgc agcgcgataa aaaagccaga gagctggagc tggcaaacgc
     1681 aaccattact gacatgcagc agcgccagcg tgatgttgcc gctggtcgta agcgcctgcg
     1741 gatcaacgcc acctgctccg gtaccgtgcg tgaagccacc ggcacctccg gaatggataa
     1801 tgcaaccggc ccccgactgg cagacaccgc tgaacgggat tatttcaccc tcagagagcg
     1861 gttgatgaca atgcagaagc aactggaagg ggcgcaggaa tatatccgca ctcagtgtat
     1921 taactagtat ttttgttatc cggagaatgc atgaagaaat tactggtaac cgtaaagcct
     1981 tttcagggaa caattccgtt ccgtattttg cagcgtggtc gtgttcttgt tgaaggttcg
     2041 ttcagtggta aatgtacgca attacactcc cggacctttc aggtgaatgc cacgaatgaa
     2101 gagctaaccg ttgagtgtac gatgaatgcc gctaaatgcc gcatggtatc cgctgcatta
     2161 cagccagtgt gttgagcgac cttattaacc atgcgcggta ttgtcgccgt atccccgcat
     2221 taacagagac cgcagcccga ccgggagact cctctgcgcg agtgtgcggg gataatcaaa
     2281 aacgatacac accggggttt accgcgttaa cggagcgcgg cgttgtcccc tcatggtcgc
     2341 tggtccggtg cgatggtgga agaaaccgga cgatgtgtta cctcgcaagc tctgttatgt
     2401 catgtgtctg atttgtgatt taagtcggat aattgtcgtt gccattaagc agaggattga
     2461 tgaccgacag ggtggcattg ttagaataag acttattctt atctgtgccg ggaatgaaaa
     2521 tgaaaagaaa ccttccgtta attattttgt tgtcttctct ggttatgggc tgtacgcaac
     2581 ata
//
LOCUS       DAJTYD010000266         2486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTYD010000266 DAJTYD010000000
VERSION     DAJTYD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.271"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(49..233)
                     /locus_tag="OO585_003892"
                     /pseudo
     CDS             complement(49..233)
                     /locus_tag="OO585_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(395..469)
                     /locus_tag="OO585_003893"
     tRNA            complement(395..469)
                     /locus_tag="OO585_003893"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Arg,seq:cct)
     gene            complement(545..1477)
                     /locus_tag="OO585_003894"
                     /pseudo
     CDS             complement(545..1477)
                     /locus_tag="OO585_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1771..>2486
                     /gene="mlaA"
                     /locus_tag="OO585_003895"
     CDS             1771..>2486
                     /gene="mlaA"
                     /locus_tag="OO585_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6631302.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNA"
BASE COUNT          583 a          602 c          618 g          683 t
ORIGIN      
        1 catcttttac ggggtgaagg tagatttcgt atgaagggta tctgttgtct cctgcaaagt
       61 cataagcgga tattttccta cggtcagtat tttttcctta ccgtcaatct tgtagcgaag
      121 ttgccatacc ttttcccgga tacaggaaca taaaggtaca ggccattacc atcgagaagg
      181 cggtatggtt tttctttcgg ctttgctgct tcaatctgct taacggtgag catgggtaaa
      241 aatccggtgg gtaaaattat tttatccact ttttacccgt catggagtgc ggctgtcaac
      301 gatctgacgc gaaccatgac gaaccgtgaa tatacggaag gcttgatatt caggggattt
      361 tgcggactgg tacggatggg agcgaactga taaatggtgt cccctgcagg aatcgaacct
      421 gcaattagcc cttaggaggg gctcgttata tccatttaac taagaggaca atgcggcatg
      481 agtatacccg ctaatggact gcggagtaag tacactgccg ctcgattgct taaaccctcg
      541 ccatttatgc tgggttttta tcatttttct taatgttttc cgcacgttct gctttttggc
      601 gtgcttctgc tttgcgcttg ttgctcatgt cgttacggat ctgtgcatga ctcattaacg
      661 cgaagataaa ggtgccgccg cagatattcc ccgctaaagt aggtagtgcg aagggccaaa
      721 tgaaatcgct ccagtgcagc gtgccgttaa acaccagata gaggatttct acagaaccga
      781 caacgatgtg ggtggtgtca cccagtgcaa taagccaggt catcaatata atcaccacaa
      841 tctttgccgc acccgctgca ggaaacatcc aaaccatagt ggcgatcagc cagccggaaa
      901 tgatcgcgtt ggcaaacatc tcgctggggg tgttcttcat cacatccatg ccgattttga
      961 caaatgcatc gtgagtttct tcattgaaga taggcatata ttcaaatgcc catgcagcaa
     1021 tacctgtccc gagaatatta cccagcagca cgacgcccca taatcgcata agtaagccga
     1081 cgttgctcat tgtcggtttt tgcatgacgg gtagtaccgc agtcacggtg ttttcggtaa
     1141 ataattgcta gcgggccata atgacgataa taaaaccaaa ggtataaccg agattctcca
     1201 acaagaagct gcccggcacg ccttccagtt cgacatgaaa tatccctttt gccagtaacg
     1261 aagcgcccat cgacagaccc gccgcaatgg ctgaccacag tagtgccatt gcgtcgcgtt
     1321 ccagctcttt ttcaccatcc tggcggatat gttcatgaat tgccatcgcc cgggagggga
     1381 gtcgatcttc atctatttct atttttttgc cgcgctcttt ttcttcgctc tcaacttcaa
     1441 tttcgtcgct gtgttgatca attttgtcgt tgtccatggt ctcttctctt gaattagcac
     1501 gtatagctaa agcgtagcgg cttttttgct cgcaactggc gggagttact ctgaaaatgt
     1561 agaaaaggcc gcgtttgcct ttttctgttt ctatagaatc aagtagccta cagggcggcg
     1621 attaccaggc tatgatcaaa tcagcaaatc agggcgtctg gacatcagtt gacgtgctgt
     1681 tacaatcgcc cacacctaaa caggcggata cggtatcgtt ccgtcatgga tggcaaactg
     1741 cataagccat aaaaaaacag ggagacattt atgaagcttc gcctgtcggc gcttgctctg
     1801 ggaactacgc ttctggtggg gtgtgcgagt tccggtacag atcagcaagg gcgttctgac
     1861 ccgttagaag ggttcaaccg caccatgtac aacttcaact tcaatgtatt agacccgtat
     1921 attgttcgac cggtcgctgt cgcctggcgt gattatgttc cgcaaccggc gcgtaacggt
     1981 ttgagcaact ttaccggcaa ccttgaagaa cctgcggtga tggttaacta cttcttgcag
     2041 ggcgaccctt atcaggggat ggtccacttt acccgctttt tcctgaacac cattttgggg
     2101 atgggcggct ttattgatgt tgcagggatg gcgaacccga aactgcaacg gactgaacct
     2161 caccgcttcg gtagtacgct tggtcattat ggcgtgggtt atgggcctta cgttcagtta
     2221 ccgttctacg gtagcttcac gctgcgtgat gacggtggtg atatggcaga tggtctttac
     2281 ccggttcttt cctggctgac ctggccgatg tctgtgggta aatggacgct tgaagggatc
     2341 gaaacccgcg ctcagctgct ggattccgat ggtctgctgc gtcagtcgtc cgatccttat
     2401 attatggtgc gcgaagcgta cttccagcgt catgatttca tcgctaatgg cggcgaactc
     2461 aaaccgcagg aaaatccgaa cgcaca
//
LOCUS       DAJTYD010000267         2479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTYD010000267 DAJTYD010000000
VERSION     DAJTYD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.272"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..402
                     /gene="cysJ"
                     /locus_tag="OO585_003896"
     CDS             <1..402
                     /gene="cysJ"
                     /locus_tag="OO585_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="catalyzes the reduction of sulfite to sulfide in
                     the biosynthesis of L-cysteine from sulfate; a
                     flavoprotein with flavin reductase activity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6631303.1"
                     /translation="FRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKDGV
                     LTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQALLE
                     VIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            402..2114
                     /gene="cysI"
                     /locus_tag="OO585_003897"
     CDS             402..2114
                     /gene="cysI"
                     /locus_tag="OO585_003897"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6631304.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            2189..>2479
                     /locus_tag="OO585_003898"
     CDS             2189..>2479
                     /locus_tag="OO585_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HCR6631305.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKL"
BASE COUNT          610 a          642 c          728 g          499 t
ORIGIN      
        1 ttccgcgcct ttatgcagca acgcgccgcc gacgaagcgc caggtaaaaa ctggctgttc
       61 tttggtaatc cgcactttac ggaagacttc ctgtaccagg tggagtggca gcgctacgtc
      121 aaagatggcg tgctgacacg tatcgatctt gcctggtcgc gcgaccaaaa agaaaaagtt
      181 tacgtacaag acaaactgcg cgaacagggc gcggagctgt ggcgctggat caatgatggt
      241 gcccacattt atgtctgcgg cgacgctaat cgcatggcga aagacgttga gcaggcactt
      301 ctggaagtga ttgccgaatt tggtggcatg gacatcgaag cggcggatga atttttaagt
      361 gagctgcgcg tagagcgccg ttatcagcga gatgtctact aatgagcgaa aaacatccag
      421 ggcctttagt ggtcgaagga aaactgacag acgccgagcg catgaagctt gaaagcaact
      481 acctgcgcgg caccattgcg gaagatttaa acgacggtct gaccggcggc tttaagggcg
      541 acaacttcct gctgatccgc ttccacggca tgtatcagca ggatgaccgc gacatccgcg
      601 ccgaacgtgc tgaacagaag ctggagccgc accacgcgat gctgcttcgc tgtcgtctgc
      661 cgggtggggt gattaccact aaacagtggc aggcgatcga caaatttgcc ggtgaaaaca
      721 ccatctatgg cagcattcgc ctgaccaacc gccagacatt ccagttccac ggcattctga
      781 aaaagaacgt caaaccggtg caccagatgc tgcactcggt cggtcttgat gcgctggcga
      841 cagctaacga catgaaccgt aacgtactct gcacctcgaa cccttacgag tcgcagctgc
      901 acgcggaagc gtacgagtgg gcgaagaaga tttctgagca tctgttgcct cgtacccgcg
      961 cgtatgcgga gatctggctc gaccaggaaa aagtcgccac tactgatgaa gaaccgatcc
     1021 ttggtcagac ctacctgccg cgtaagttca aaaccacggt agtgatcccg ccgcagaacg
     1081 atatcgatct gcatgccaac gacatgaact tcgtggcaat cgccgaaaac ggcaagctgg
     1141 taggcttcaa cctgctggtg ggcggtgggc tttctatcga acacggcaac aagaaaacct
     1201 acgcccgcac ggcgagcgag tttggctatc tgccgctgga gcatacgctg gcggtggccg
     1261 aagctgtcgt gacaactcag cgtgactggg gtaaccgaac cgatcgtaaa aatgccaaaa
     1321 ccaaatacac gctggagcgc gtgggggttg agacgtttaa agcggaagtg gagcgtcgcg
     1381 cggggatcaa atttgaaccg atccgtccat atgagttcac cggacgaggc gatcgtattg
     1441 gctgggttaa gggcattgat gataactggc acctgacgct gtttatcgaa aatggtcgca
     1501 tccttgatta tccggggcgt ccgctgaaaa ccggcctgct ggagatcgcg aagatccaca
     1561 aaggcgattt ccgcattacg gcgaaccaga atctgatcat cgccggtgta ccggaaagcg
     1621 agaaagcgaa gatcgagaag atcgccaaag agagcgggtt aatgaatgcc gtcacgccgc
     1681 agcgtgaaaa ctcgatggcc tgcgtgtcat tcccgacttg cccgctggcg atggcggaag
     1741 cagagcgttt cctgccgtct tttatcgaca acatcgataa tttaatggcg aaacatggtg
     1801 tcagcgatga gcatatcgtg atgcgtgtaa caggctgccc gaacggttgt ggtcgcgcga
     1861 tgctggcgga agtgggcctg gtgggtaaag cgccgggtcg ctacaacctg catcttggcg
     1921 gcaaccgcat tgggacacgt atcccacgga tgtataaaga aaacatcacc gagccggaaa
     1981 tcctggcgtc gcttgatgaa ctgatagggc gctgggcgaa agagcgcgaa gcgggtgaag
     2041 gcttcggcga ctttacggtg cgtgcgggca tcattcgccc ggtgctcgat ccggcgcgcg
     2101 atttgtggga ttaaccatca gcccggtctt gtaggcctga taagaacgcg tgagcgtcgc
     2161 atcaggcaag gcaaacagtg aggaatctat gtccaaactc gatctaaacg ccctgaacga
     2221 actgccgaag gtagatcgca ttctggcgct ggcggaaact aacgccgaac tggaaaaact
     2281 ggacgctgaa ggccgcgtag cctgggcgct ggataatctg cccggtgaat atgtactttc
     2341 ttccagcttt ggcattcagg cggcggtgag cctgcatctg gtgaatcaaa ttcgcccgga
     2401 tattccggtg atcctcaccg atacgggtta cttgttcccg gaaacctacc gctttattga
     2461 cgagttaacg gacaaactc
//
LOCUS       DAJTYD010000268         2451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTYD010000268 DAJTYD010000000
VERSION     DAJTYD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.273"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            545..1084
                     /locus_tag="OO585_003899"
     CDS             545..1084
                     /locus_tag="OO585_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6631306.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(1310..1633)
                     /locus_tag="OO585_003900"
     CDS             complement(1310..1633)
                     /locus_tag="OO585_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6631307.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1917..2111
                     /locus_tag="OO585_003901"
     CDS             1917..2111
                     /locus_tag="OO585_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6631308.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          834 a          577 c          512 g          528 t
ORIGIN      
        1 ttatcgccac tggcagcagc cactgataac cgaattagag gagtaagact tgaagtcatg
       61 cgcctgttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      121 acctcggctc aaagagttgg tatctctgag aaccttcgaa aaaccgcctt gcagggcggt
      181 tttttcgttt acaggcagta gattacgcgc agaaaaaaag gatctcaaga agaaccttta
      241 attttttttc acacttggta tcctacttta acccataaaa taaatactta caccgacacg
      301 cccttaaaca aaagaaaaaa aaacaataaa aaacaccgca aaaatggatt gttaacaaat
      361 gaaaatactc aattcgaatc tcataacaaa catatcatca acaataagaa atcaccttct
      421 aacatcatcc aaaaatataa aaacacaccc atcaccaaac atggacgtaa caagtcatcg
      481 tcgtttattc cagaaagacg gaataggatt gagccaaaca ggaaaagcaa cacaacttgt
      541 cattatgtcg catggtggat ggaaagagat ctcccatcca caaacactat tcacgcgtca
      601 aaaaggcgat ggatggactg tagtccccaa aaacctaaga attgattttt atacaaaaga
      661 caacgacttc acaaagggac taagcgttct ttcagaggta aacaaacgcc atactgaagc
      721 tcaaaaaggg ttaacaccat cactaaacat aagcaaagat gacctaaaag ttctagcgaa
      781 cgcgcgcaat atacctcaat caaaattaga agaagaaatg atgtcacaag ccatatatag
      841 aaaagaatac gcaacaagta atgagaagat aaaaaactat gctctatatt accatgaaca
      901 agcccaaaat attatacaaa gacatcaaga tggcttagga aacaaaaata ttgatatagc
      961 atttataact gataaaaatc ataagaagca cctttctgac atatttaaag taataaatga
     1021 atcaggagta aaatatgatg taattcactt tggcgcttgc cgagtaaata gagaggagaa
     1081 ataatcatca tttaccataa tcccttaatt gtacgcacca ctgaaacgcg ctgaacgcga
     1141 ttctgacgca gacaccacaa aaatagatgt tttaccccgg aaaaacagga aggatgcgtc
     1201 aggatcgttt tcagctcgct gcatagctat gcatgaaagc gaatggagat cactttggga
     1261 gcttaccgtg ttcataccgt ctgttttcaa cagttttctc cccgggaagc taatctgcca
     1321 taagcctgga taacagggca cggtgatact ccgtaatagc gatcagcacc tccgcatact
     1381 ccctgtcccc gatacgcttg ccgtcgataa agagtttttt ctgtaacgcc cccagtctca
     1441 taacttcttt cagtacccga tcatcagtca gtgagttaac gttcttaccg agagctgccg
     1501 cccgtaaaaa accggaaacg gtcttgccgc attcagcagc cctttttctg atttcctggt
     1561 cttcttctgc cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac
     1621 ttcgttttgt cataaaacat ggctccgtat ctgacatggg tgtcggggca aagccctgac
     1681 cagggcaatt gtaatagcgt gcatgtatgc gcggtataac aattgcacat cctgtcctgt
     1741 cagcaagctc gaattaccga caaaggcacg gcagtttttt ggcagttaca gtgttgtctg
     1801 acagaaatgc ccgtcaaaaa gccaggcggc agaatagcgg cagcaaaaca gtcgaaaagt
     1861 ggtacgcgat ataatcacat aacgcagatg acagacagaa cggagaaaac gaaaagatga
     1921 acaagcagca gcaagccgta ctcaacatgg cgggattcat aaaaagccag agcctgacgc
     1981 tgctcgaaaa actggacgca ctcgatgctg acgagcaggc caccatgtgt gagaaactac
     2041 acgaactcgc agaagaactc cagaacagca tacagacacg ctttgaagta gaaaatagca
     2101 cagagagata acgctcagga caggatgtcg aacggcacaa cagagcgtaa ggagaaaatg
     2161 tcacataagg cgctcttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     2221 gcggcgagcg gtatcagctc actcaaaggc ggtaatgcgg ttatccacag aatcagggga
     2281 taacgccgga aagaacatgt gagcaaaaag ccgaaggcag ggacaaaggc cgcgcaggtg
     2341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg a
//
LOCUS       DAJTYD010000269         2441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTYD010000269 DAJTYD010000000
VERSION     DAJTYD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.274"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..557
                     /locus_tag="OO585_003902"
     CDS             <1..557
                     /locus_tag="OO585_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6631309.1"
                     /translation="KIDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQ
                     NTFIFTEFNPAKTKYFILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGF
                     NATWGGEKPVDAKEFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKL
                     AINMENIQFDNLPSAENRQKRSKK"
     gene            601..>2441
                     /locus_tag="OO585_003903"
     CDS             601..>2441
                     /locus_tag="OO585_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6631310.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENA
                     TAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIA
                     LGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVG
                     NSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTY
                     NLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSK
                     DAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADAL
                     LWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIAN
                     NTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSK"
BASE COUNT          750 a          545 c          582 g          564 t
ORIGIN      
        1 ataaaattga tctacttgaa aaacaacaag cacagctaaa gagtcaactg gaaactattc
       61 aaaaacaaca aactggcata ataagcagta caaagacttt aactcacgta attaaatccg
      121 ttaaagatca acaaaacact tttattttta ccgagtttaa cccagcaaaa acgaagtatt
      181 tcatcctgaa caatggttcg gtggctttag cgggtcgagt gttatccatt gacgccacag
      241 agaatggcag tgttattcat atttcactgg tcaacttatt aagtacacct atctcaaata
      301 ttggttttaa tgcgacatgg ggtggtgaaa aacctgtcga tgccaaagag tttgcccggt
      361 ggcaacaatt acttttcaac acatcaatga aatccacatt gaaattatta ccaggtcaat
      421 ggcaagacat taatttgacc ctgaagggtg tctcgcccaa taacctggga tatctgaagt
      481 tagccatcaa catggaaaat attcagttcg acaatcttcc ctctgctgaa aatcggcaga
      541 aaagaagcaa aaaataaatg cagttagtac acttataaca aggaatagcg agcatcatta
      601 atgaacaaaa tatttaaagt tatctggaac cctgcgacag ggaattatac tgttaccagc
      661 gaaacggcaa aaagccgtgg caagaaatct gggcgcagta agctgttaat ttctgcgctg
      721 gttgcgggtg gaatgttgtc gtcgtttggg gcattggcga atgccgggaa tgacaacggt
      781 cagggtgttg attacggtag tggatcagct ggcgacggct gggttgctat aggcaaaggg
      841 gcgaaagcaa atacttttat gaacaccagt ggttccagta ctgctgtggg ttatgacgct
      901 atagctgaag gccaatatag ctctgccatc gggtcaaaaa cccatgcgat tggtggtgca
      961 tcaatggcct ttggggttag tgcaatatca gaaggcgata gaagtatagc actgggtgcc
     1021 tcttcgtatt cattgggcca atactcaatg gccctcggcc gttattcaaa agcattgggt
     1081 aaattgtcta ttgctatggg ggactcttcc aaagcggaag gagcaaacgc cattgccctg
     1141 gggcgtagca gtgtagctag cggtactgac agcctcgcat ttggcagaca atcacttgcc
     1201 agcgcagcga acgctattgc gataggtgct gagaccgaag ccgctgaaaa tgcaactgct
     1261 attggcaata atgcgaaggc aaaagggact aatagcatgg caatggggtt cggaagcctt
     1321 gccgataaag tcaatactat cgcattagga aatggcagcc aggctctggc agataatgca
     1381 atcgccatag gccagggcaa caaagctgat ggcgtggatg ccatcgctct gggtaatggt
     1441 agccagtcga gaggcttaaa caccattgcc ttaggcacag ccagtaatgc aactggtgat
     1501 aagagtcttg cgcttggtag taatagcagt gccaacggta ttaactctgt cgcgctgggc
     1561 gcagattcca ttgcggattt agacaatacc gtctctgtcg gcaatagttc attaaaacgc
     1621 aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc cattaatggt
     1681 tcacaacttt atgccattag cgactcggta gcaaaaaggc ttggaggagg ggctgcagta
     1741 gatgttgatg acggtactgt tacagcacca acctacaatt taaaaaatgg tagcaaaaat
     1801 aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga ccaaaccaaa
     1861 ggcaaataca gcgctgctca tggtactagt agcccaactg ccagcgtaat caccgatgtt
     1921 gcggatggca cgatttcagc ctccagtaag gatgcggtta acggttccca actgaaagct
     1981 accaatgacg atgtcgaagc caacaccgcc aatatcgcta ctaataccag caacattgcc
     2041 acgaatacgg caaatattgc caccaatacc accaatatca ccaacctgac ggattccgtt
     2101 ggtgaccttc aggctgatgc cctgctctgg aacgaaacta aaaaggcatt cagtgcagct
     2161 cacggccagg ataccaccag caaaatcacc aacgttaaag atgccgacct gacggctgac
     2221 agcactgatg ctgttaacgg ctctcagctg aaaaccacca acgatgctgt ggcgacgaat
     2281 accaccaata tcgccaataa cacttccaat attgccacta acaccaccaa catctctaat
     2341 ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt
     2401 gtattcacgg cagctcatgg caccgagacc accagcaaaa t
//
LOCUS       DAJTYD010000270         2420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTYD010000270 DAJTYD010000000
VERSION     DAJTYD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.275"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            203..2392
                     /locus_tag="OO585_003904"
     CDS             203..2392
                     /locus_tag="OO585_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR6631311.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT          533 a          559 c          627 g          701 t
ORIGIN      
        1 caaccctcgc tgcaaagcgg gggttttttg tctctgcttt ttgccgcttt tgtaatacag
       61 tctacgtccg ggttagtgcc gcctggtgaa agcatcattg gatgaaaaat cggcaacagg
      121 ctggccccct gtttgcttcg cgatgcgaat aaacttatta tttgtgtgcc tgaaaactcc
      181 gatcagtgag agtagtgtac tcatgtttgt ggagcataac ctgataaaaa atatcaagat
      241 attcacacta gcgtttacgc tcaccgtggt acttattcag ctatcccgtt ttatttcgcc
      301 acttgccatt atccattcca gttatatctt tctggcgtgg atgccactgt gcgtaatgct
      361 gtcaatcttg tttatctttg gctggcgagg tgtcgttccc gttttatgcg ggatgttttg
      421 caccaatctg tggaactttc atctctcttt tttacagact gcggtcatgc tcggtagcca
      481 gacgtttgtc gtgttgtgtg cctgcgcaat attacgctgg cagctgggga cgcgttggcg
      541 ttatggattg accagccgat atgtctggca acgtctgttc tggcttggtt tggtgacgcc
      601 gatcggcatc aaatgcagca tgtatcttgt ggggagcttc tttgatttcc cgctaaagat
      661 atccaccttt ttcggcgatg cggatgccat tttcacggtc gttgatttgc taagcctttt
      721 caccgctgtg ctgatttaca acatgctttt ctactatctc actcgcatga ttgtaagtcc
      781 ccactttgcg cagatattgt ggcgcaggga tatcgcgccg tcgttgggca aagagaaacg
      841 cgcatttacc ttaagctggc tggcagcgtt aagcgtgctt ctacttctgt tgtgcacacc
      901 ttatgaaaac gactttattg ccggttacct ggtacccgtt ttcttcatca tctttaccct
      961 cggggtcggt aagcttcgct atccgttttt aaatctcacc tgggctgttt caacgctttg
     1021 ccttctgaat tacaaccaga actttttgca aggggtggaa accgaatatt cgctggcatt
     1081 tattcttgcg gtgctgattt cctttagcgt ttgcctgctc tatatggtgc gcatttatca
     1141 tcgcagtgaa tggcttaatc gccgctggca tttgcaggcg ctgaccgatc cgttaacgct
     1201 cctgcccaac ttccgtgcgt tggaacaagc gccggagcaa gaggcgggca agagtttttg
     1261 ctgcctgcgc attgataatc ttgagtttat gagtcgtcat tacggcttaa tgatgcgcgt
     1321 tcactgtatc cgctcaattt gccgtacgct gctgccgttg atgcaggaaa acgaaaagtt
     1381 gtatcaattg ccgggtagtg aactgctgtt agtgctgagc gggccggaaa cggaagggcg
     1441 actccagcat atggttaaca tcctgaatag tcggcaaatt cactggaaca ataccgggct
     1501 ggatatgggc tatggtgctg cctgggggcg ttttgatgga aatcaggaaa ccctgcaacc
     1561 cttgttgggg cagttaagct ggctggcgga gcaatcctgc gcacatcatc atgtgctggc
     1621 gctggatagc agagaggaga tggtttccgg gcagaccact aaacaggtgc tattgctgaa
     1681 taccattcgc acggcgttag atcagggtga tttgctgctc tacgcccagc caattcgcaa
     1741 caaagagggt gaaggttatg atgagatcct cgcgcgactg aaatatgacg gcggcattat
     1801 gaccccggat aagtttctgc cccttattgc tcagtttaac cttagcgcgc gttttgattt
     1861 gcaagtgctg gaatccttgt tgaagtggct ggcaacacac ccttgcgaca aaaaaggacc
     1921 gcgcttttca gtcaatttaa tgccgctcac gctgctgcaa aagaatattg ccgggcggat
     1981 tattcgtctg tttaagcgtt atcacatctc cccgcaggcg gtcattcttg agatcaccga
     2041 ggagcaggcg ttttctaacg cagaaagcag catgtacaac atcgagcagc tgcataagtt
     2101 tggtttccgg attgcgattg atgactttgg caccggctat gccaactacg aacggttaaa
     2161 gcgtttgcag gctgatatca tcaaaattga tggcgtcttt gtgaaagata ttgtcacgaa
     2221 cacgctggat gcgatgattg tgagatcaat taccgatctg gcgaaagcga agtcattgag
     2281 tgtggtcgcg gagtttgtcg agacgcaaca gcagcaggcg ctattgcata agctcggggt
     2341 gcaatatctg caagggtatt tgattggtcg cccgcagcca ttagctgatt aacaggcgta
     2401 aaaaaaacgg ggaattatcc
//
LOCUS       DAJTYD010000271         2319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTYD010000271 DAJTYD010000000
VERSION     DAJTYD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.276"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..2000
                     /gene="iha"
                     /locus_tag="OO585_003905"
     CDS             <1..2000
                     /gene="iha"
                     /locus_tag="OO585_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCR6631312.1"
                     /translation="ASGYEKKLTNAAASVSVISQEELQSSQYHDLAEALRSVEGVDVE
                     SGTGKTGGLEISIRGMPASYTLILIDGVRQGGSSDVTPNGFSAMNTGFMPPLAAIERI
                     EVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKWGNSSQFNFWSS
                     GPLVDDSVSLQVRGSTQQRQGSSVTSLSDTAATRIPYPTESQNYNLGARLDWKASEQD
                     VLWFDMDTTRQRYDNRDGQLGSLTGGYDRTLRYERNKISAGYDHTFTFGTWKSYLNWN
                     ETENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGESHLVTVGGEFQSS
                     SMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHEQFGGHFSPRAYL
                     VWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPDLKPEESVSYEAG
                     VYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHGVEFAGTLPLWSE
                     DVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVASWLGARYRGKTP
                     RFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNAAVNNLLNKDYSD
                     VSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(2218..>2319)
                     /locus_tag="OO585_003906"
     CDS             complement(2218..>2319)
                     /locus_tag="OO585_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6631313.1"
                     /translation="PETWLRHVISVINTWPANRMKELLPWNVTLSVN"
BASE COUNT          604 a          519 c          665 g          531 t
ORIGIN      
        1 cggcatccgg ctatgagaaa aagctgacta acgcagccgc cagtgtttct gtgattagcc
       61 aggaggaatt gcagtccagc cagtaccacg atctggcgga ggctctgaga tcagtagagg
      121 gtgtggatgt tgaaagtggt acgggtaaaa ccggtgggct ggaaatcagc atccgaggaa
      181 tgccagccag ttacacgctg atactgattg atggtgttcg tcagggcgga agcagtgacg
      241 tgactcccaa cggtttttct gccatgaata ccgggttcat gccccctctg gccgccattg
      301 agcgtattga ggttatcagg gggccgatgt ccacactgta tggctctgat gcgatgggcg
      361 gtgtggtgaa tatcattacc agaaagaatg cagacaaatg gctctcttcc gtcaatgcag
      421 ggctgaatct gcaggaaagc aacaaatggg gtaacagcag ccagtttaat ttctggagca
      481 gtggccccct tgtggatgat tctgtcagcc tgcaggtacg cggtagcaca caacagcgtc
      541 agggctcatc ggtcacatca ctgagcgata cagcagccac gcgtattcct tatcccacgg
      601 agtcacagaa ttataatctt ggtgcacgtc ttgactggaa ggcgtcggag caggatgtgc
      661 tctggtttga tatggatacc acccggcagc gttatgataa ccgggatggg caactgggga
      721 gtctgacggg gggatatgac cggaccctgc gctatgagcg gaacaaaatt tcagctggct
      781 atgatcatac tttcaccttc ggaacatgga aatcgtatct gaactggaac gagacagaaa
      841 ataaaggtcg tgagctcgta cgcagtgtac tgaagcgcga caaatggggg cttgccggtc
      901 agccgcggga gcttaaggaa tcgaacctta tcctgaattc attactgctt acccctctgg
      961 gagaatctca tctggttacg gtggggggcg agtttcagag ctcgtccatg aaagacggag
     1021 ttgtccttgc cagcacaggt gaaactttcc ggcagaaaag ctggtcggta tttgctgagg
     1081 atgagtggca tctcacggat gcacttgcgc tgactgcggg cagccgctat gaacatcatg
     1141 agcaattcgg gggacacttc agtccgcgtg catatctggt ctgggatgtg gcagatgcct
     1201 ggacgctgaa aggcggtgtg accacgggat ataaggcacc cagaatgggg cagctacata
     1261 aagggattag tggtgtgtcc gggcagggaa aaacaaatct acttggtaac cccgacctga
     1321 agccggaaga gagcgtcagt tatgaggctg gggtgtatta cgataacccc gccggtctga
     1381 atgccaatgt cacaggtttt atgactgact tctccaacaa gattgtctct tattccataa
     1441 atgataacac caatagctat gtaaacagcg gaaaggcccg gttgcacggt gtggaatttg
     1501 ccggcacatt gccgctgtgg tcagaggatg tcacgctgtc actgaattac acctggaccc
     1561 gaagtgaaca acgtgatggt gataacaaag gtgcgccgct gagttatacc cctgaacaca
     1621 tggtgaatgc gaaactgaac tggcagatca ccgaagaggt ggcatcatgg ctgggtgccc
     1681 gttatcgcgg gaaaacacca cgtttcaccc agaattattc gtcactgagc gctgtacaga
     1741 agaaagtgta tgatgagaaa ggagaatacc tgaaagcctg gacggtggtg gatgcaggtc
     1801 tgtcctggaa gatgacggat gccctgacgc tgaatgctgc ggtgaataac ctgctcaaca
     1861 aggattacag tgacgtgagc ctgtacagtg ccggtaagag tacgctgtat gccggtgatt
     1921 acttccagac gggatcatca acaacaggat atgtgatacc tgagcgaaat tactggatgt
     1981 cgctgaacta tcagttctga taataacaaa acgctatcac tgacggtaga atacgttgcc
     2041 actgcaactc ctggcggaac agtggcaacg tattaggtta agtgcatttc cgatccgcta
     2101 atgagatttc gttaccaaca actaatatcg tcacaggaaa tgcacggatt atttttaact
     2161 tatcatttac atacttgtcc agagtgtaag cgcaccgtga aggacgtggg gtaaaaatta
     2221 gtttacagag agagtgacgt tccagggcaa caactctttc atgcggttgg caggccaggt
     2281 gttgattaca ctgatcacgt ggcgtagcca cgtttccgg
//
LOCUS       DAJTYD010000272         2301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTYD010000272 DAJTYD010000000
VERSION     DAJTYD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.277"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO585_003907"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO585_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6631314.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO585_003908"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO585_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6631315.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO585_003909"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO585_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6631316.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          617 a          490 c          541 g          653 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agcaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaggg caacagatcg cgtacccagt
     1021 ttgccggcca gtcttggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattca ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatacgataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aaccacggca gcctggcggc
     2161 tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa tgagttccca
     2221 tgtcacacaa cctggagcac cagaaagtcc acaccagaat ggtgaaagag gttctgaaag
     2281 ccgtcgccag agccaataat c
//
LOCUS       DAJTYD010000273         2270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTYD010000273 DAJTYD010000000
VERSION     DAJTYD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.278"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1763)
                     /locus_tag="OO585_003910"
     CDS             complement(<1..1763)
                     /locus_tag="OO585_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631317.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSG"
     gene            complement(1774..>2270)
                     /locus_tag="OO585_003911"
     CDS             complement(1774..>2270)
                     /locus_tag="OO585_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631318.1"
                     /translation="IATANTRDRGVNEMSAALKRRFDFETVFPIMDFAQELELVASAS
                     ARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEKKTSMDTLTAIMSTAEAVNVAHAVG
                     VRAWFLANRAGEPADLVDCIAGTIVKDNEEDRARLRRYFEQRVATHKEAHWQAYYQAR
                     HRLP"
BASE COUNT          503 a          624 c          622 g          521 t
ORIGIN      
        1 ccgctatgta gatcgcgcag aatatggcaa agcgcttgcc agctatgcag gtgagcgtga
       61 aactcctttt catccggtct gccgttagat tcacacagcc acagcgtgcg atcgatagcc
      121 gcgcataacg tcgtttgtaa tatctgcgcg ccttgcatac cgctaatttc atccagccgc
      181 cataaggcat atagcacttc cagcgcctgg cccatttcgg caaattgatt ttcctgggcg
      241 attaataatg tgagttgctc cagtaattgt tgactgaagg acgccagacc cgctaacgcc
      301 gcctggctaa gacatgtggt gatactgccg atgccacccg cgtccagcat atcggcttct
      361 aatttatggc gtgcggcttc ctgaagtgtt gcaccaaagc atgcggcctc aatcaatgcc
      421 gcatgctggc taagcgggcg ggttaatttc cagtgttctt caccgttgcc tgcaagtgtc
      481 agtgtacttc cctgctggcg tacaatccca gggatctcca gtattgccag ccgatgtaac
      541 acctgacttt gcgccagccc atcgggggta aagcgattta aggtaagctc cgccggaaga
      601 gagattgcgg tacgttccag ttcgcaggtg acatcttttg gcagaggcgg ttgtggcgtg
      661 gaaggggcaa gtttaccgaa tccgtcaccc gctaatgtgt ctatcaacgt tagcagaatc
      721 ggatcggtat cgggatgaat aacgccgcga tagctccacg gcaacggtgc attcagggct
      781 tctttaatca gcgagcctgc tatggcatcc agccagtcag tgcgtaacgg tagtgtatga
      841 ccgcgcaact gtgccagtgc cattgcatgc agatgtgcgg cagccatatc cgcgatcgaa
      901 gcaggcaagt tgtgctggcg caaacgggtg agaaccgttt ttagcagttg ttcaccggcc
      961 tgctgtaagc cccactgcca gcaccagttt tgccagaccg gggcaggcat accggaaagg
     1021 tatcctgcca gcacatcaag gtgcttttca ctgtaggggg tgagataaca acctgtaacg
     1081 gcatttgcca gcgagggcaa ttctggcttg ttaatttcct gcggacattc gcgccacatt
     1141 tttgccagtg caggagcgtg ccagccaccg cagacgacta acacgtcgcc attatttcgc
     1201 tgcaccgccc atgcaatcca gcgagccata aaggcttcgc gctgacgatt gagtgcatcg
     1261 ccggggaaat cgccccgcag ttgggcaaaa tagtgcgcca gcgcagaggg taatgcaatt
     1321 tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc catgcgggtg
     1381 gcacgcagca gcaaggcctg gctttcctct tgcgtatcag gcgaatcgtc cacttcttca
     1441 ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg acgtgcggct
     1501 tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc acgcccgggg
     1561 gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg gtgggctaaa
     1621 aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaatac gtatcgtggc
     1681 cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg atgccggatg
     1741 ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc gggcttgata
     1801 ataagcctgc cagtgagctt ctttatgtgt ggcgactcgt tgttcgaagt aacggcgcag
     1861 acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt ccaccaggtc
     1921 ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg cgtgggccac
     1981 gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg ttttcttttc
     2041 gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg cgtcaggcac
     2101 tttatgcgga ataccgctat gagccagcaa acgcgccgaa gcactggcga ccagttccag
     2161 ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac gctttagcgc
     2221 agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa
//
LOCUS       DAJTYD010000274         2244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTYD010000274 DAJTYD010000000
VERSION     DAJTYD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.279"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..898
                     /locus_tag="OO585_003912"
     CDS             <1..898
                     /locus_tag="OO585_003912"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6631319.1"
                     /translation="DVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNKAWL
                     LSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDMNIS
                     NAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCVKVR
                     DRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVYYSD
                     ISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALSCEY
                     TNNPDLSIANVISLVENAERLN"
     gene            865..1824
                     /gene="psuG"
                     /locus_tag="OO585_003913"
     CDS             865..1824
                     /gene="psuG"
                     /locus_tag="OO585_003913"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6631320.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1918..>2244
                     /locus_tag="OO585_003914"
     CDS             1918..>2244
                     /locus_tag="OO585_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6631321.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVT"
BASE COUNT          573 a          496 c          599 g          576 t
ORIGIN      
        1 tgatgtcgcc ggatattcac atgaatcatt aaattatgcg gattcaaatc caggtaaaat
       61 aaaatttacg cctggtggag tcgggcgcaa tattgcacaa aacctggcgt tgctggggaa
      121 caaagcctgg ctactgagcg ccgtaggcag tgacttttat ggtcaatcgc tgctaacgca
      181 aaccaatcaa tctggcgttt atgtcgataa atgcctgatt gtgccgggag aaaatacgtc
      241 tagttattta tcattactcg ataataccgg tgaaatgctg gttgctataa atgacatgaa
      301 tattagcaac gctattacag ctgaatatct cgcacagcac cgtgaattta ttcagagggc
      361 aaaggtcatt gtcgcggact gtaatatcag tgaagaggca ctggcatgga ttctggataa
      421 tgccgccaac gtacccgtat ttgtcgatcc ggtttccgca tggaaatgtg tcaaagtgcg
      481 cgaccgtcta aatcagatcc acactctgaa gccaaaccgc cttgaagcgg aaaccctgag
      541 tgggattgcg ctgtcagggc gtgaagatgt ggcaaaagtt gctgcctggt tccatcaaca
      601 tggcctgaac cgactggtat tgagcatggg cggcgacggc gtttattaca gcgatatcag
      661 cggtgaaaat agctggtctg cgccgatcaa aaccaatgtt attaatgtta ccggagcggg
      721 cgatgccatg atggcgggac ttgcttcgtg ttgggtagac ggaatgccgt ttgccgaatc
      781 tgttcgtttc gcacagggat gttcgtcaat ggcgctctcc tgtgaataca ccaataaccc
      841 cgatttatcg attgccaacg ttatatcgtt agtggagaac gcagaacgtc tgaattaaaa
      901 atttcccctg aattattaca aatttccccg gaagtgcagg acgctttaaa aaacaaaaaa
      961 ccggttgtgg cgctggaatc gaccattatt tctcacggga tgccgttccc acaaaatgcc
     1021 cagaccgcaa ttgaagtaga agaaactatt cgtaaacagg gcgctgtacc tgccactatc
     1081 gccattattg gcggcgtgat gaaagtgggt ttaagcaaag aagaaattga attactgggt
     1141 cgtgaagggc ataacgtgac caaagttagt cgtcgcgatt taccttttgt tgttgccgcc
     1201 ggaaaaaatg gcgcaaccac tgtggcttca acgatgatta ttgcggcgct tgccggaatt
     1261 aaagtatttg ccaccggggg aattggtggt gtgcatcgcg gggcggaaca taccttcgat
     1321 atttctgccg atttgcaaga actggcaaat actaatgtca ccgttgtttg tgccggggcg
     1381 aaatctattc tcgatttagg attaaccact gagtatttag aaaccttcgg tgtgccgtta
     1441 attggctatc agactaaagc gctgcctgcg tttttctgcc gtaccagctc gtttgacgtc
     1501 agtattcgtc tcgacagcgc cagtgaaatt gcccgtgcaa tggcggtgaa atggcaaagc
     1561 gggctgaacg gtggcctcgt ggtagcgaac ccgatcccgg aacagtttgc gatgccggag
     1621 gaatctatca atgcagccat agatcaagcc gtcgccgaag ccgaagagca gggcgttatt
     1681 ggtaaagaaa gtacactgtt cctgctggcg cgcgttgctg agctgaccgg cggtgacagc
     1741 ctgaaatcca atatccagct ggtattcaac aacgccattc tggcgagtga aattgccaaa
     1801 gaataccagc gtctcgcggg ttaatttccg acgggcagaa agccgtcgcc cgtactcatc
     1861 aagcatacca aaacctggcg ctgacgccgg gtttactggc atgaagggaa aatcattatg
     1921 gatataatga gaagtgttgt ggggatggtg gtgttactgg caatagcatt tctgttgtca
     1981 gtgaataaaa agagcatcag tttgcgcacg gttggagccg cactgctgct gcaaatcgct
     2041 attggtggca tcatgctcta cttcccaccg ggaaagtggg cagtagaaca ggcggcatta
     2101 ggcgttcata aagtgatgtc ttacagtgat gccggtagcg ccttcatttt tggttcgctg
     2161 gttgggccga aaatggatgt cctgtttgac ggtgcgggtt ttatcttcgc ctttcgcgta
     2221 cttccggcga ttattttcgt tact
//
LOCUS       DAJTYD010000275         2237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTYD010000275 DAJTYD010000000
VERSION     DAJTYD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.280"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..555)
                     /locus_tag="OO585_003915"
     CDS             complement(<1..555)
                     /locus_tag="OO585_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HCR6631322.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNI"
     gene            844..1794
                     /gene="tal"
                     /locus_tag="OO585_003916"
     CDS             844..1794
                     /gene="tal"
                     /locus_tag="OO585_003916"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6631323.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            1814..>2237
                     /locus_tag="OO585_003917"
     CDS             1814..>2237
                     /locus_tag="OO585_003917"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6631324.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDH"
BASE COUNT          557 a          575 c          552 g          553 t
ORIGIN      
        1 gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga tgattgccgt
       61 gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt tctgttcaat
      121 atagaaacat tctggcgctt taatgtcttc gaggttgatt ccgccgaagg tcggttcgag
      181 cgcagcgaca acttcaataa atttgtccgg gtcgagttca tcaacttcaa tgtcaaatac
      241 atcaatcccg gcgaatttct taaacagaac gcccttgcct tccatcaccg gtttgccagc
      301 cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca ccgccaccag
      361 gttaccgcgc gcggtatatt tgtaggcttt taacgggtct ttttcgattt caagacaagg
      421 tgcggcaacg cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca gaggcttggt
      481 tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg cactttgttt
      541 taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa gtgtgtgggc
      601 aacagcccct gcccacaacg tggcgcacat tattaccccg ccggagtcta cagactttga
      661 gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata acactgatgt
      721 tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg cgtttagcga
      781 aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca aggagtattt
      841 cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga cagcggcgat
      901 attgagtcca ttcgccatta tcatccccag gatgccacca ccaatccttc gctgttgctc
      961 aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc ctggggtaaa
     1021 aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact ggcggtcaat
     1081 ttcggtgctg aaattctcaa aatcgtaccc ggtcgcgtgt caacagaagt tgatgcacgc
     1141 ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga cttgtatcag
     1201 caacaaggcg ttgagaaatc acgcattctg atcaaactgg cttcgacctg ggaaggaatt
     1261 agcgcggcag aagagctgga aaaagaaggt attaactgca acctgacgct gctgttttct
     1321 tttgcccagg cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc gtttgtcggg
     1381 cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt ggaagaagat
     1441 ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca ctatgaaacc
     1501 attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac cggctgcgat
     1561 cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc gccagtggta
     1621 cgtaaattaa tcccaccttc tcagacgttc ccacgcccag ctcccatgag cgaagcggag
     1681 ttccgttggg agcacaatca ggatgcgatg gcggtagaaa aactgtctga aggcattcgt
     1741 ctgttcgccg ttgatcaacg caaactggaa gatcttcttg ccgccaaact ataaaccagc
     1801 cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac tcagtatgga
     1861 tgcggtacaa aaagccaact ctggtcatcc cggcgcgccg atgggcatgg ctgatattgc
     1921 cgaagtgctg tggaacgatt ttcttaaaca taaccctacc gacccaaccg ggtatgatcg
     1981 cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt tgctgcatct
     2041 gaccggttac gacctgccgc tggaagagct gaagaacttc cgtcagttgc attcgaaaac
     2101 cccaggccac ccggagattg gttatacgcc cggtgttgaa accaccaccg gtccacttgg
     2161 acaaggtttg gcgaacgccg tcggactggc gatagcagag cgtacactgg cggcgcagtt
     2221 taatcagccg gatcatg
//
LOCUS       DAJTYD010000276         2234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTYD010000276 DAJTYD010000000
VERSION     DAJTYD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.281"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..609
                     /locus_tag="OO585_003918"
     CDS             <1..609
                     /locus_tag="OO585_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="catalyzes the reduction of trimethylamine-N-oxide
                     to form trimethylamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCR6631325.1"
                     /translation="KNEQYVRYADFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPA
                     HPTWLAPDEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDA
                     ARFGIANGDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVL
                     TADIPSSQLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(774..1745)
                     /gene="cmoB"
                     /locus_tag="OO585_003919"
     CDS             complement(774..1745)
                     /gene="cmoB"
                     /locus_tag="OO585_003919"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6631326.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(1742..>2234)
                     /gene="cmoA"
                     /locus_tag="OO585_003920"
     CDS             complement(1742..>2234)
                     /gene="cmoA"
                     /locus_tag="OO585_003920"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6631327.1"
                     /translation="IIAIDNSPAMIERCRRHIDAYKAPTPVDVIEGDIRDIAIENASM
                     VVLNFTLQFLEPSERQALLDKIYQGLNPGGALVLSEKFSFEDAKVGELLFNMHHDFKR
                     ANGYSELEISQKRSMLENVMLTDSVETHKARLHKAGFEHSELWFQCFNFGSLVALKAE
                     DAA"
BASE COUNT          568 a          566 c          575 g          525 t
ORIGIN      
        1 aagaacgaac agtacgttcg ttatgctgat ttccgcgccg atccggtgaa aaatgcgctg
       61 ggtacgccaa gcggcaaaat tgagatttac tccaaaacgc tggaaaaatt tggctataag
      121 gattgcccgg cacatccaac ctggcttgcg cctgatgagt ggaagggtac cgccgacgag
      181 aagcagttgc agcttctgac cgcacatccg gcacaccgtt tacatagtca gcttaactat
      241 gcggaactgc gtaaaaaata tgcggttgca gatcgtgaac caatcactat tcataccgaa
      301 gatgctgctc gctttggtat tgcgaatggt gatctggtgc gtgtgtggaa caaacgtggt
      361 cagattctga caggcgcggt ggtgactgac gggatcaaaa aaggcgtggt atgcgtgcat
      421 gaaggtgcat ggccagatct ggaaaatggc ttgtgtaaaa acggcagtgc gaacgtgtta
      481 acggcggata tccccagctc gcagctggca aatgcctgtg ccggtaactc tgcgctggtg
      541 tatatcgaaa aatatacggg taacgcaccg aagttaacgg catttgataa gcctgctgtt
      601 caggcgtaag aagtgaatag cgcccggaaa cgggcgctga ggttatgcaa acgtaaaact
      661 gcctgatgcg ctatgcttat caggcctaca tagtccctgc aatagattga attagcaagg
      721 gtttgtaggc cggataaggc attcacgctg catccggcag taataccaga cctttacggc
      781 ttgcgcgcaa tcagcaccgc gcgtttaggc gcaggataac cttcaactgt cttactcgga
      841 tcatgtgggt cgaggaaatc ggacagagat tcggtaacca tccattcagt gcgtcgctgc
      901 tcttcagtag tggtaacgca cacatccaca acgcggatat ctacaaaacc acacttcttc
      961 agccagtttt tcagcgccag cgcggaaggg atgaaataga cattacgcat ttgcgcgtaa
     1021 cgatcgcctg gtaccagcac cgtgttctca tcgccatcaa taaccagcgt ttccagcacc
     1081 agttcaccct cattcaccag ttgatctttc aactgccaga gatgctccag cggcgaacgg
     1141 cgatggtaaa gcacgcccat cgaaaagacg gtatcaaagg ctttcagtgc cggaagctgt
     1201 tcaataccta acggtaacaa atgtgcgcgc tgatcgttac ccagtagttt acgcaccgct
     1261 tcaaactggc acagaaacag ttgcgtaggg tcgatcccca ccgcgaggtg cgcgcctgcg
     1321 ccaatcatgc gccacatgtg ataaccgctg ccgcagccga catccagaat ggtgcgtccg
     1381 gttaaatcag aaagatgggg cagaacacga tcccatttcc agtcggaacg ccattcggta
     1441 tcgatgttga cgccatacag tgagaacggc cctttgcgcc atggcatcag gttccgcata
     1501 agcgtttcaa tgcgtttaat ttgcccggtg ctcagtggct cttcgctttc ggcggttacg
     1561 ctatgcaata aatccagacg atacggttta atttcaggca gaaattccac cgcgttggac
     1621 cactgcttaa acagcccgtg ctgctgctcg cgctgccagt tagcaatctg cgcgggcagc
     1681 gtttcgagcc agtgtgaaag atgatttttg gcaatcagag aataaaagtt accaaagtcg
     1741 atcatgcagc gtcctctgct tttaatgcca ccagtgaacc aaagttaaag cactggaacc
     1801 acagctcgct atgctcaaaa ccggctttat gcaggcgtgc tttatgggtt tccacggaat
     1861 cggtcagcat cacgttttcc agcatgctgc gcttctggct gatctccagt tcgctgtaac
     1921 cgttggcacg tttaaagtcg tggtgcatgt tgaacaacag ttcaccaact ttggcatctt
     1981 cgaaactgaa tttttccgaa agcaccagcg cgccgcccgg gttcagtcct tgataaattt
     2041 tatccagtaa tgcctggcgc tcggaaggtt ccaggaattg cagggtaaaa ttcagcacca
     2101 ccatcgatgc gttttcaatg gcgatatcgc gaatatcacc ttcaatgacg tctactggcg
     2161 taggggcttt ataggcgtca atatgacgac ggcagcgttc aatcatcgcc ggggagttgt
     2221 cgatggcaat aatt
//
LOCUS       DAJTYD010000277         2218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTYD010000277 DAJTYD010000000
VERSION     DAJTYD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.282"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..689
                     /locus_tag="OO585_003921"
     CDS             <1..689
                     /locus_tag="OO585_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HCR6631328.1"
                     /translation="ALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAVLKRYDD
                     EGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRHYLAPSD
                     KLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQRICKEL
                     GKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELATKPVKL
                     PEKVRKVEAA"
     gene            complement(732..1178)
                     /gene="ivy"
                     /locus_tag="OO585_003922"
     CDS             complement(732..1178)
                     /gene="ivy"
                     /locus_tag="OO585_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6631329.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            1359..2129
                     /gene="yafV"
                     /locus_tag="OO585_003923"
     CDS             1359..2129
                     /gene="yafV"
                     /locus_tag="OO585_003923"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6631330.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          498 a          546 c          623 g          551 t
ORIGIN      
        1 tcgccctgct ttctgatgtc tcgatggcag tgctgggcgg cagcctgaaa cgtcgcgagc
       61 gcatctcggc ccgtctgggg gatattttaa gccagctcta cctcgcctct gccgtgctga
      121 agcgttatga cgacgaaggc cgtaatgaag ccgacctgcc gctggtgcac tggggcgtac
      181 aagatgcgct gtatcaggct gaacaggcga tggatgattt actgcaaaac ttcccgaacc
      241 gcgtggttgc cgggctgctg aatgtggtga tcttcccgac cggacgtcat tatctggcac
      301 cttctgacaa gctggatcat aaagtggcga agattttaca agtgccgaac gccacccgtt
      361 cccgcattgg tcgcggtcag tacctgacgc cgagcgagca taatccggtt ggcttgctgg
      421 aagaggcgct ggtggatgtg attgccgccg acccaattca tcagcggatc tgtaaagagc
      481 tgggtaaaaa cctgccgttt acccgtctgg atgaactggc gcacaacgcg ctggcgaagg
      541 gcctgattga taaagatgaa gccgctattc tggtgaaagc tgaagaaagc cgtctgcgca
      601 gtattaacgt tgatgacttt gatccggaag agctggcgac gaagccggta aagttgccgg
      661 agaaagtgcg gaaagttgaa gccgcgtaat tgaataacgg agccgaaagg ctccgtttct
      721 ttatccgcta attatttaaa attaaagcca tccggatggt tttccaggct gccggtcaac
      781 gccgcgaaca gcaccgtttt accatcaatc gaaagcgcat cgttcacatt cagccaggtg
      841 agtttctctt gcgacgtttt ctcatcaata gtcgagaaca gccccgtcat ctgattagat
      901 ttctcggacc acatcacagc gatacgttgc gagccacagt catgcggttt gcacgcgctc
      961 atcacctgat acgtctcatc tcccaacgtt acggtttgtg cgggagtata agtaccgcct
     1021 ttcatcaccc aggcaggcag cttatgccct tgtaccatct gattaaatgc agctttggtg
     1081 gtttcgccct ttgcaaggct gctaatggtt aaatcatcct gcgccattgc actggtggcg
     1141 atgaccagag cggcgactgt cgttattgcc ttaaacatca ttcctcccga gcttatcctg
     1201 cccatgttat taaccttcta aagcctggcg ataacacgat aatttgcaaa tcagatattt
     1261 ccaatatcag catttgttat caatttattc tgcgctaagg caactgcctg ataaggtgtt
     1321 aaagttataa aacccttcgc ataaaggagt tgtacaccgt gcctggtttg aagattacgc
     1381 ttttgcagca accactggtg tggatggatg gccctgccaa cctgcgtcat tttgatcgtc
     1441 aactggaagg tattaccggg cgcgatgtga tcgttctacc ggagatgttt accagcggct
     1501 ttgccatgga agcggcagct tcgtcgctag cacaagatga cgtagtgaac tggatgacag
     1561 ctaaggcgca gcagtgcaat gcgctgatag caggcagtgt tgcattacaa acggagtctg
     1621 gttcggttaa ccgctttttg ctggttgagc cgggcggcac ggtacatttt tatgataagc
     1681 gtcatctgtt ccgcatggca gatgagcatc tacattataa agcgggcaat gcgcgagtga
     1741 ttgtggaatg gcgcggctgg cgtattttgc cgttggtgtg ctacgactta cgttttccgg
     1801 tgtggtcgcg caatctcaac gattatgacc tcgccatcta cgttgccaac tggcctgctc
     1861 cgcgctctct gcactggcag gcattgctga cggcccgcgc gattgagaat caggcgtatg
     1921 tggcgggatg caatcgcgtc ggcagcgatg gcaacggctg ccattatcgc ggtgacagcc
     1981 gggtgattaa tccacaaggc gagattatcg ctactgccga cgcgcatcag gcaacgcgca
     2041 ttgatgcgga gctgtcgatg atggcattgc gggaatatag agaaaagttt ccggcatggc
     2101 aggatgcgga tgagtttagg ttgcggtgag tgggggttgt gtttaaggct gggggagagt
     2161 cggggtaaga ctacgaaagc ccgctctccg caaggactga cgccagatag tttctgtc
//
LOCUS       DAJTYD010000278         2186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTYD010000278 DAJTYD010000000
VERSION     DAJTYD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.283"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..190)
                     /locus_tag="OO585_003924"
     CDS             complement(<1..190)
                     /locus_tag="OO585_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6631331.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSV"
     gene            complement(436..888)
                     /locus_tag="OO585_003925"
     CDS             complement(436..888)
                     /locus_tag="OO585_003925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6631332.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(885..1937)
                     /gene="dinB"
                     /locus_tag="OO585_003926"
     CDS             complement(885..1937)
                     /gene="dinB"
                     /locus_tag="OO585_003926"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6631333.1"
                     /translation="MKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTAN
                     YPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLD
                     EAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ
                     FVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFG
                     RILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKV
                     KPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLH
                     VTLLDPQMERQLVLGL"
BASE COUNT          568 a          544 c          510 g          564 t
ORIGIN      
        1 caacagagaa gaacgctgcg ccaatcggat agccgcgtcc gggatgtaaa tctggcatcc
       61 ccactacgcg ctgcatgttg ggtaaatttg ccgtggtgtg taattgttgg atcgctaaac
      121 tctcgatcca ctggtcatca gatgcgatgg taaataccgc atcggataag ggacgaatat
      181 aattgcccat ataccattcc gtaaatagac tcgtcatact tcaagttgca tgtgttgcga
      241 cttgaattat tgagagtaac aattaaaaaa tcaggaaatg gcagacgaat ccagggcaag
      301 aaaaaaataa ttaatcctgg aaaggtctgc aaagagtcat tgataaagga tttgtcatga
      361 tttacctcct tgcagtttgt ggaataagaa aacgcgcaca tactaggctt gcgttatttt
      421 cattgcaagc tggatttaat gttgctgttt ataacgcata taaaaattaa taaaccactc
      481 tccccgacat tcaacgcgtt gctgcttaac tgtctgaaaa ccataacgtt caaaaaaggg
      541 ttttgcggtt atgcttgcgt ccaccgtaag ttcggattca gacttaatca aaggttttaa
      601 caaagcgctg gcaaccccac ggcgggtgta ttcagggtca acaaataaca tatcgatata
      661 gtgttcaacg caggtaataa agccgaccag ttttgcatta atgactgcaa cccgcacttg
      721 tgatttcgcg agtttctcct tccagcgaga ttcgtcaatc tgcgcccagg cggcaatttg
      781 ttgtggtgaa taatgctgac tggcagtcat cgtaaccgct ctaatgaaaa tagcgcatag
      841 ttgctgaaaa tcgccaggct gatagtctct tatttgtatg ttattcataa tcccagcacg
      901 agttgtcttt ccatttgcgg gtcaagcaac gtcacatgca gccccaccag tcgcacaccg
      961 cgcccgccgc ggcgttcatc ccaggtttta cgcgcggtgg cgattaagtc agctttattc
     1021 agccgcggcc agacgtgctc ctgagtggtt tgctgaaaat catcaaactt taatttcacc
     1081 ccctggcgag caatcagtaa atcaggtttc acctttgcca gacggcgttc aagttccgga
     1141 tacagccgct cgataatcgc ttcacattca gaccagtggt ggatatcttc cgccatcgtg
     1201 cgttccacgc cgacggattt tcgcaaccgt tcgctgttaa cgtcgcgctc gtcaatcccc
     1261 tgactacgct cccacaaaat gcggccaaat ttgccaaagc gtttaagcag catcaccaga
     1321 tcacactttt gtacatcacc gcaggttcgt agccccatcg cttccagttt tgccgccgag
     1381 actttgccga cgccggggat ttttgccagt ggtaaggttt gtaaaaatgc cggaacttct
     1441 gccggcgtaa tcacaaactg gccgttgggt ttattcatgt cggaggcgat tttggcgaga
     1501 aactttacgg gtgccacgcc cgcagacgcc gtcagttgca gctcgttgaa aatcgtctgg
     1561 cggatttcct gggcgatgag ggtcgcagaa ccgtggcaat ggacgctatc ggtgacgtcg
     1621 agataagcct catccagtga caacggttca atacgcgagg tgtagcgcga gaagatttcg
     1681 cggatatgat ttgaggcttc tttgtaggcg tcaaagcgcc ccggaagcaa ggtgagatgc
     1741 gggcataatt tgagcgccat ccctgtcggc atagcgctac gtacgccaaa tttacgcgcg
     1801 ggataattgg cggtactgat caccccccga cgttcgcggc tgccgccaat agcaataggg
     1861 atatcgcgca gggcgggatt gtcgcgcatc tccaccgccg cgaaaaagca gtccatatcc
     1921 acatgaataa tcttcacttt cttatacacc ctgtatattt atacatgcaa agagattata
     1981 cacacaaaag caaaatcaat aagtgccctt cttattcgga ttcacagagt acttccgtaa
     2041 agcattaccc gacttaagca cctttgaagc ttgctcatta gctgaggctg tgaaggtgct
     2101 ctcaccattt ttaacgctaa cgctcttaga gcacgttaca cgggctttta gcgcttcatc
     2161 tgataactca cttgccgaat agctaa
//
LOCUS       DAJTYD010000279         2123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTYD010000279 DAJTYD010000000
VERSION     DAJTYD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.284"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..640
                     /locus_tag="OO585_003927"
     CDS             <1..640
                     /locus_tag="OO585_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCR6631334.1"
                     /translation="NIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAAL
                     VFSIDAPLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDM
                     NNLYKWLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFA
                     FTAFACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(641..1549)
                     /locus_tag="OO585_003928"
     CDS             complement(641..1549)
                     /locus_tag="OO585_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6631335.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            1649..>2123
                     /locus_tag="OO585_003929"
     CDS             1649..>2123
                     /locus_tag="OO585_003929"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCR6631336.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGI"
BASE COUNT          564 a          456 c          509 g          594 t
ORIGIN      
        1 taatatcccg gatgacttaa tgactaacgg tcaatattac gcctttcaga agctgggcga
       61 gtattacaac atgggtaata ctttaatggt gatttacgcc attgcgaata ccctgggaca
      121 agtagctgcg ttggtattct cgattgatgc cccgcttaaa gtgctattag gtgatgctga
      181 cagcaaatat attccagcca gtttatgtcg taccaacgct tctggtacgc ccgttaatgg
      241 ctattttctg accctggtgc aggtggcaat tctgattatg ctgccgactc tcggcatcgg
      301 tgatatgaac aatctctata aatggctgtt gaatcttaac tcggtagtga tgccgctgcg
      361 ttatttatgg gtatttgttg catttattgc agtcgttcgc ctggcgcaga aatataaacc
      421 agaatatgtc tttattcgta ataagcctct ggcaatgacc gtcgggattt ggtgttttgc
      481 ctttaccgcc tttgcctgtt tgacggggat cttcccgaaa atggaagcct tcactgcaga
      541 gtggaccttc cagttggcgc tgaatgttgc aacgccgttt gtgctggtag gattaggact
      601 gattttcccg ctgctggcgc gtaaagcgaa tagtaaataa ttattgtggt cggtgacgct
      661 gactctggct gatctgaccc gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca
      721 tatactggcg gtttggataa tagatctgga agcctggacg ctctgtgctc cagtctccca
      781 gtacacattc taaacgacca gtatcaaggt actccttgat ctgttcatag agcagatacc
      841 cgataccagc acccatcaaa acggcctcca gttcagcatc aacatcatcc agaatgatat
      901 ttcctgcgac agcaatttcc agctcctttg caaattgcca gtggaaaggt ttgccgctgg
      961 gataacgaaa aacaacacat tgatgattaa gcagatcatg aggatggcgg ggtttgccat
     1021 atcttgcaaa gtactctggc gttgctgcta cacataattt cactggcggg ccgatcgcca
     1081 cgctgatcat atctttttcg acgatgccac taagtcgaac gccagcgtca aagccctgtt
     1141 ggacgatatc gacaagagag tcgtcagttg tgagctcgac tttaatatca ggatattcac
     1201 gtgtgaaacc aaccagtaaa ggcattaaaa atatacgtgc agctacccgg gcgtcattga
     1261 ttttgagcgt tcctgtcggt gtcagacgaa aatcattcat ctcatctaac atgatctgaa
     1321 tttcgtcgaa agctgggcgc aatcgttcat agagattaga acccgcttcc gttaacgata
     1381 cgcttcgggt tgtccgattg aataagcgaa ttttaagacg ttgttccagt gttttaatac
     1441 tatggctaat ggcggacgag gataagccta actcatcgcc cgctgcacga aagctttgat
     1501 tacgggccac ggcaaagaaa gtggcaaagt cagacatatt catccgcatt agtgaaaatc
     1561 cttcattaac tcatcctgaa tcatcacgtt tacaggatta tgccgcctgc gtaaagtgcg
     1621 ctccagaact taacgtggag gtaaaattat gcagtctgaa cgtatttatt tggtatgggc
     1681 ccatcctcgt catgattcat tgaccgcaca tattgctgat gcgatccatc agcgggcaat
     1741 ggagcggaaa atacaggtga cggaactcga tttatatcgg cgtaatttca acccagtgat
     1801 gacgccggaa gatgaaccag actggaagaa tatggataaa cgttattctc cagaggttca
     1861 tcagctttat tcagagctgc ttgaacatga cacgttagtg gtggtttttc ctctctggtg
     1921 gtacagcttc ccggcaatgc taaaaggata tattgacaga gtatggaata atgggctggc
     1981 ttatggagat gggcacaaat taccattcaa taaagttcgt tgggtggcgc tggttggagg
     2041 agacaaagaa tcatttgtcc agatgggctg ggaaaaaaat ataagcgatt atttgaaaaa
     2101 tatgtgcagt tatcttggta ttg
//
LOCUS       DAJTYD010000280         2042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTYD010000280 DAJTYD010000000
VERSION     DAJTYD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.285"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>2042
                     /locus_tag="OO585_003930"
     CDS             <1..>2042
                     /locus_tag="OO585_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6631337.1"
                     /translation="KKITFTANAEQLLQPQFTVEQLRELGIKVDEIPALAEKDDDSVI
                     NSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARGYVSPSRWDDGIPTLFTNYSFTG
                     SDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTRNDQASSWNTISSYLQRDIKAL
                     KSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNSQRGFAPTVRGIANSSAIVTIRQ
                     NGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEESDGTQRRFIQPYSSLPMMQRPRH
                     LKYSATAGRYRADANSDSKEPEFAEATAIYGLNNTFTLYGGLLGSEDYYALGIGIGGT
                     LGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKDIPETNTNIAVSYYRYTNDGYFS
                     FDEANTRNWDYNSRQKSEIQFNISQTIFDGVSLYASGSQQDYWGNNEKNRNISVGVSG
                     LQWGIGYSLNYQYSRYTDENNDRALSLNLSIPLERWLPRSRVSYQMTSQKDRPTQHEM
                     RLDGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGV
                     TGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVIPYLTTYQE
                     NRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLP"
BASE COUNT          558 a          502 c          474 g          508 t
ORIGIN      
        1 agaaaaaaat tacatttacc gccaatgcag agcaacttct gcagccacag tttacggtag
       61 aacaactacg tgagctgggt attaaggtgg atgaaatccc ggcgctggct gaaaaagatg
      121 acgatagcgt gatcaactcg cttgaacaaa tcattcccgg tacagctgct gaatttgatt
      181 tcaatcatca gcgacttaat ttgagcattc cccaaattgc actgtaccgt gatgcaagag
      241 gttacgtctc cccttctcgt tgggacgatg gtataccaac gctgtttacc aactactcgt
      301 ttacaggttc tgataaccgt taccgccagg gcaatcgtag ccaacgacag tacctgaata
      361 tgcaaaatgg tgctaatttt ggcccctggc gattacgtaa ctattctacg tggacacgca
      421 acgatcaggc gtcaagctgg aacactatca gtagttattt acaacgtgat atcaaggcgt
      481 tgaagtctca gttgcttctg ggagaaagcg ccaccagcgg cagtattttt tccagctaca
      541 cctttactgg cgtgcaactc gcttccgacg ataatatgtt gccaaacagc cagcgcggat
      601 ttgccccaac ggtacgcggt atcgcaaaca gtagtgcaat cgtgactatc aggcaaaatg
      661 gttatgtgat ctatcaaagc aacgtgccag cgggtgcctt tgaaattaac gatctctacc
      721 cctcttccaa cagcggcgat ttagaagtca cgattgaaga aagtgacggt acacaacgtc
      781 gctttatcca gccttattct tcattaccca tgatgcagcg acctaggcat ctaaaatata
      841 gcgcgaccgc tggacgctat cgcgctgatg caaacagtga tagcaaggaa cccgaatttg
      901 ctgaagccac ggcaatatat ggtttgaata atacttttac gctgtatggc ggcctgctcg
      961 gttctgaaga ttattatgcg ctggggatcg gtatcggcgg cacacttggc gcactgggcg
     1021 cgttgtcgat ggatatcaac agagctgaca cccaattcga taaccagcac tcttttcatg
     1081 gctatcaatg gcgtacgcag tacatcaaag atatcccgga aaccaacacc aatatcgctg
     1141 ttagctacta tcgctatacc aacgatggct attttagttt tgatgaagcc aatacccgca
     1201 attgggacta taacagtcgc caaaaaagtg aaattcaatt caacatcagc cagacaatat
     1261 ttgatggggt aagtctgtat gcctccggtt cgcagcaaga ctattggggc aataacgaga
     1321 aaaacaggaa tatctctgtt ggggtttccg gcctgcaatg gggaattggt tacagcctga
     1381 attatcaata cagccgctac actgatgaaa ataatgaccg tgcactctct ttgaatctca
     1441 gtattccgtt agaacgctgg ttaccgcgta gccgggtttc ctatcagatg accagccaga
     1501 aagatcgccc aacccaacat gaaatgcgtc ttgatggctc actgctggat gatggtcgcc
     1561 tgagctatag cctggaacaa agtctggatg acgataacaa ccataacagt agcctgaacg
     1621 ccagttaccg ttcaccttat ggaaccttca gtgccggata cagttacggt aatgacagca
     1681 gccaatacaa ttacggcgtt accggcggcg tggttatcca tcctcatggc gtgacgctct
     1741 cgcaatatct gggcaacgct tttgcgctta tcgatgctaa cggggcttct ggcgtgagga
     1801 tacaaaacta tccggggatt gctactgatc cctttggcta tgcagtgatt ccttatctca
     1861 cgacttatca ggaaaaccgt ctctcggtag atactacgca gctgcccgat aacgtcgatc
     1921 ttgaacaaac aacacagttt gtggtgccca acagaggtgc aatggtagcg gcgcgtttca
     1981 acgccaatat cggttatcgc gtacttgtta cagtcagcga tcgcaacggt aaaccgttgc
     2041 cc
//
LOCUS       DAJTYD010000281         2031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTYD010000281 DAJTYD010000000
VERSION     DAJTYD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.286"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..150
                     /gene="gmhA"
                     /locus_tag="OO585_003931"
     CDS             <1..150
                     /gene="gmhA"
                     /locus_tag="OO585_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="catalyzes the isomerization of sedoheptulose
                     7-phosphate to D-glycero-D-manno-heptose 7-phosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HCR6631338.1"
                     /translation="LTGKDGGKMAGTADIEIRVPHFGYADRIQEIHIKVIHILIQLIE
                     KEMVK"
     gene            356..1123
                     /locus_tag="OO585_003932"
     CDS             356..1123
                     /locus_tag="OO585_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6631339.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1094..1834)
                     /locus_tag="OO585_003933"
     CDS             complement(1094..1834)
                     /locus_tag="OO585_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6631340.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
BASE COUNT          511 a          512 c          517 g          491 t
ORIGIN      
        1 ctgaccggta aagacggcgg caaaatggct ggcacggcgg atatcgaaat tcgcgtaccg
       61 cactttggtt atgccgaccg cattcaggag attcacatta aagtgatcca tatcctgatc
      121 cagttaattg aaaaagagat ggttaagtaa gtctggcgta ggccggataa ggcgtttacg
      181 ccgcatccgg cacttgtgcg ctgatgcctg atgcgatgct gacgcgtctt atcatgccta
      241 caaatctgta cgcgaaccgt aggccgaata attcgtttac gccgcatccg acctgaaaat
      301 tcttaaatca atcttcgccg ggggccatgc gctcccgctg ttgtggaggt tacccatgtg
      361 cgaattgctc gggatgagcg ccaacgtccc taccgatatc tgctttagtt tcaccggact
      421 tgtacagcgt ggtggtggaa ccgggccaca taaagatggc tggggcatta ccttttacga
      481 aggtaaaggc tgtcgcacat ttaaagatcc acaacccagc tttaattccc ccatcgccaa
      541 acttgtccag gactacccga taaaatcctg ttcggtggtg gctcatattc gccaggctaa
      601 tcggggcgag gtggcgttgg aaaatactca cccatttacc cgcgagttat gggggcgtaa
      661 ctggacttat gcccataacg gacaactgac gggctacaaa tcactggaaa ccggcaactt
      721 ccgcccggta ggcgaaaccg acagcgaaaa agccttctgc tggctgctgc ataaattaac
      781 gcagcgttac ccgcgcacgc cgggcaacat ggcagcagtg tttaaatata tcgcttcact
      841 ggcggacgaa ctgcggcaga agggcgtttt caacatgctg ctttcggatg ggcgctatgt
      901 aatggcgtat tgctcgacga atctgcactg gatcacccgc cgcgcaccgt ttggcgtggc
      961 aacgctgctg gatcaggatg tagaaatcga cttcagctcg cagaccacac cgaatgatgt
     1021 ggtcacggtg attgcgacac agccgctgac gggcaatgaa acctggcaaa agattatgcc
     1081 aggcgaatgg cgcttatttt gcctcgggga gcgtgtagtt tgatgccagt tgtggctgca
     1141 caacttcgtg gcttaacggc ttgctgacca cgtaacgacc attgaccaca gaaacggtcg
     1201 gtggcttacg ggtttgctca aagtagtcgt aacccggctt cagttgctcc cagaagtcct
     1261 taaagttgga atatttatgg cgcttcatat tggcgtcggt catgcggaac gggtaaatac
     1321 tcacttgcac gctcggctga ccaaacacca gcgcaccagt aacgaactgg aagatctcat
     1381 caataccctg attggtcatt gcgtagcagc cgatggaaac acaatcgccg tggatcatca
     1441 ggtatttccc ttcataacca tgcgcacggt cataggcatt ggggaaacca atattaatcg
     1501 ctttgtagta acggctgtct ggttttaact gattacgctg gacgctataa aacccttccg
     1561 ggcttttgaa atcgccctga cgctgttttg gccctaagcc gccggaatat ttacagattt
     1621 tataactgtc gagcagctga tattgctcgc ccattttgac gtagagatcg agcgtacgtt
     1681 cttccttgaa gatctggatg tagacagggg atcccatcaa ctgctgctta tactctttgc
     1741 tcactggcgt ggtcgagcta ctgctgccca gcaaaccggc aaacgaaacg cacgggatca
     1801 acaacatcgc aagaattaat gcgattttac gcatactgct tattccttga taaaacggtt
     1861 acacacgcca ggacggcaaa atggatccca aatcggaata gtctggattt ggaaggctca
     1921 cattatcacc aaaagagttt tacgcaagcc tgtcggcgca gggtttacaa atttagcaca
     1981 ggggcagttt tcaatagtcc gaaaagcgca tgtatcattt atttcctaag a
//
LOCUS       DAJTYD010000282         1976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTYD010000282 DAJTYD010000000
VERSION     DAJTYD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.287"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            547..1536
                     /locus_tag="OO585_003934"
     CDS             547..1536
                     /locus_tag="OO585_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6631341.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          439 a          527 c          585 g          425 t
ORIGIN      
        1 aaaaaaaatt cggcgtcccg ctcactgttc ccgaactcgt caaacagaaa atcgcggggg
       61 cccctccggg gttccgattt tgtgattgac gacggcacgg aaaaaaacac tggggatggt
      121 ggttggtgtc acgcagtgag gccaaccacc tgcaagacgg tttttgtttt tcacaaaatc
      181 gctggaaaaa cctgtgagtg ttttgcgggg aaaacgcttt tgtggttgtt gtttttgctg
      241 tccgaaaatc agctcagatt ttgagcaaat ctcgcgcgcg ttccttgtat ttatacttaa
      301 gggataactc taggattcaa attggcctcc agcccttgtc ccgcaaggcc tgagggggga
      361 tttgtcggaa aatggttttg gcgttattct gtcgggagtg gttgcgttaa aagtgacccc
      421 cgcaacgacg ggcatcgttc gggggtgtga ccacctgtag aaattcgaat tagtgacacg
      481 acaacacagg tgatatggca atgataacac gtcagatgag ccatcgccca caatcgggtg
      541 gtgcgcatga aaaaccctaa actccagtac ctcactgatt attccccctc tgatgcgccc
      601 tgggacgcac acagaagcgt ttctgacgac gtgggaggga tttacctgct tgcggccgaa
      661 tatgagcgct acggggctcg tatggcctcc tgtggcggtt tactgcgttt cgggtggtct
      721 accctcaagg aaaccggaga aacgcgtctg aggctgcgtg aggcacattt ctgccgtgtc
      781 cggcactgtc ctgtctgcca gtggaggcgc tccctgatgt ggcaagcccg tttctaccag
      841 tccctgccgc gtatcgtggc ggattatccc gatgcccgct ggatgttcct gaccctgacc
      901 gtgcgcaact gtgcgattgg ggaactgggc gagatgctga accggatgaa tgcagctttt
      961 cagcgtatga aggttcgcaa ggagttcctg cctgttcagg gctggatccg cacaacggaa
     1021 gttacccgcg gcagcgacgg cagcgcacac ccgcacttcc ataccttgat gatggctcct
     1081 cctggcatgc taaatgggaa aagttacgtc aggcatgaac gctgggttga gctatggcgg
     1141 gagtgcctgc gggttgatta tgacccgaat gttgatgtcc gggcagtgaa accccgcaag
     1201 ccgaaggatg gcgagtcctt agcctgcgcg accgctgaac tggtgcgcgg cgcagtggcc
     1261 gaaaccctga aatattccac gaagccggct gacatggtgg ccgatcctga atggttcctc
     1321 gaactgaccc gccagacgca caagcgccgc tttgtggcga ccggcggcgc actcaaagac
     1381 gtgttgaaac tcgatcagga aaccgacgcc gacatggtga tcggggatga tatttccgag
     1441 ggcgacgatg acggttcacg aatcgcgttc gagtggaaaa ccgaggtcag aaagtaccgt
     1501 cgctcaccga cgaaagataa aactgaatca ggctagcggg gtgtcggggc gaagccctga
     1561 ccaggtgggg aatgtctgag cgagcgtgcg cggtccgaca ttcccacatc ctgtcccgaa
     1621 aattcccgcc gtttctcggt tttttgagcg tcgcagaccg cacgacaaac gaaacgaagt
     1681 gtagtttgta tgagtgcgca cttcgtgaga ttccccgact gccagtttgt cgatcgcgcg
     1741 gaacagctgc ggcacgaaaa cggcagaatg atttgctgtc cggttgccag aactgtgcgc
     1801 ttacgtggag ggcggcgcgg cgcaaaaact gccgacattg cgccagtgtt ccggtgacac
     1861 aaacccgcag aaatcgggac aggatgtgta atcgtataac cgcgcacgca cagccatccg
     1921 attaccacct ggtcagggct tcgccccgac accccgccaa cgcttcacgg cacgcg
//
LOCUS       DAJTYD010000283         1910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTYD010000283 DAJTYD010000000
VERSION     DAJTYD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.288"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(123..>1910)
                     /gene="glyS"
                     /locus_tag="OO585_003935"
     CDS             complement(123..>1910)
                     /gene="glyS"
                     /locus_tag="OO585_003935"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6631342.1"
                     /translation="ARGCGITVDQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATS
                     LAKLPIPKLMRWGASDVHFVRPVHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPE
                     FTIDNADQYPEILRERGKVIADYEERKAKIKADAEEAARKIGGNADLSESLLEEVASL
                     VEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDP
                     QQIISGNEKVVRPRLADAEFFFNTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQA
                     LAGWIAEQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAV
                     ALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALG
                     VLRIIVEKNLNLDLQTLTEEAVRLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVD
                     TIQAVLARRPTRPADFDARMKAVSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVN
                     ASTLKEPEEIKLAMQVVVLRDKLEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVD
                     DKELRLNRLTMLEKLRELFLRVADISLLQ"
BASE COUNT          438 a          538 c          485 g          449 t
ORIGIN      
        1 aatctcataa aattaatata tgagataatc tttattcagc agaagattat taaaggttgc
       61 tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta ataacggcgt
      121 tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag tttctccagc
      181 atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac tttatcgaag
      241 aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg gtaacgacct
      301 tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc cagtttaatt
      361 tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc gtcagatttc
      421 gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc atccagggta
      481 cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt cggacgacgc
      541 gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca ggcgcggaag
      601 cgaccgagca taaagtcgat aacatcatca accacgttgg cgttagtcag cttatcgcca
      661 tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt cttctcaacg
      721 ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt gtcgcctttc
      781 ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc aatcgccagc
      841 gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg atactgctca
      901 ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg catccccata
      961 acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca cttagacagc
     1021 aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat ccagccagcc
     1081 agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg gaacagcacg
     1141 gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt gaagaagaac
     1201 tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat ttgctgcgga
     1261 tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc atcgttcgca
     1321 tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc agccggcacc
     1381 gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac cagcgaagcc
     1441 acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg cgctgcttct
     1501 tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac tttcccgcgc
     1561 tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg ttcgcccata
     1621 aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatagttgc cggaatgact
     1681 ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac gtcgcttgcg
     1741 ccccaacgca tcagtttcgg gatcggcagt ttcgccagag aagtcgcaac catattcggc
     1801 agcagtgctt cggtgctttc gcccttcaca tgggcgcgat acagcagcca ttcgccttta
     1861 tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
//
LOCUS       DAJTYD010000284         1899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTYD010000284 DAJTYD010000000
VERSION     DAJTYD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.289"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OO585_003936"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OO585_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6631343.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1520)
                     /locus_tag="OO585_003937"
                     /pseudo
     CDS             complement(1149..1520)
                     /locus_tag="OO585_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1519..1899
                     /locus_tag="OO585_003938"
                     /pseudo
     CDS             1519..1899
                     /locus_tag="OO585_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          596 a          339 c          377 g          587 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttg
//
LOCUS       DAJTYD010000285         1712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTYD010000285 DAJTYD010000000
VERSION     DAJTYD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.290"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            175..474
                     /locus_tag="OO585_003939"
     CDS             175..474
                     /locus_tag="OO585_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6631344.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            569..1480
                     /gene="glyQ"
                     /locus_tag="OO585_003940"
     CDS             569..1480
                     /gene="glyQ"
                     /locus_tag="OO585_003940"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6631345.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            1490..>1712
                     /locus_tag="OO585_003941"
     CDS             1490..>1712
                     /locus_tag="OO585_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6631346.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRG"
BASE COUNT          418 a          447 c          441 g          406 t
ORIGIN      
        1 ctgtgtctct gtatctggtt aaaaaaagtc tgaccgataa atcatttggt tggcgcactg
       61 gaataatctg agttttatca ctacagctta tagaggctta aggaaattcg taagatatca
      121 gccactatac cgatataaat aataagactc acctgcaaac cagacggtaa tttaatgatg
      181 atgaacgctt tctttccggc aatggcgctt atggtgctag tgggttgttc tacaccgtca
      241 cccgtgcaga aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa tatggaagcg
      301 ttatgcaagg atcaggcggc aaaacgttat aacaccggcg agcaaaaaat cgacgtcacc
      361 gccttcgaac agttccaggg aagctatgaa atgcgcggtt ataccttccg taaagagcag
      421 tttgtctgtt cttttgacgc agatggccat tttttgcatc tttccatgcg ttaagccctg
      481 ctttttcccg tttcgtactg tatatcttcc atccagcggg tatactgatc ccttccttta
      541 aatccacacg tatccagcac gaaataatat gcaaaagttt gataccagga ccttccaggg
      601 cttgatcctg accttacagg attactgggc tcgccagggc tgcaccattg ttcaaccatt
      661 ggacatggaa gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg cgctggggcc
      721 agaaccgatg gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg gtcgctacgg
      781 cgaaaacccc aaccgtttac agcactacta tcagttccag gtggtcatta agccatcgcc
      841 ggacaatatt caggagctgt acctcggttc tctgaaagag ctgggcatgg acccgactat
      901 tcacgacatc cgtttcgtgg aagataactg ggaaaacccg actctgggtg cctggggact
      961 gggctgggaa gtgtggctaa acggcatgga agtgacgcag ttcacttact tccagcaggt
     1021 tggtggtctg gagtgtaaac cggttaccgg cgagatcacc tacggtctgg aacgtctggc
     1081 catgtacatt cagggcgtag acagcgttta cgacctggtc tggagcgacg gcccgctggg
     1141 taaaaccacc tacggcgacg tgttccatca gaacgaagtg gagcagtcca cttacaactt
     1201 cgaatacgcg gatgtggact tcctgttcac ctgctttgag cagtacgaga aagaagcgca
     1261 gcagctgctg gcgctggaaa atccgctgcc gctgccagcc tacgagcgta ttctgaaagc
     1321 cgcccacagc ttcaacctgc tggatgcgcg taaagccatc tccgtcaccg agcgtcagcg
     1381 ctacattctg cgcattcgca ccctgaccaa agcagtggca gaagcatact acgcttcccg
     1441 tgaagccctc ggcttcccga tgtgcaacaa agataagtaa gaggcggcta tgtctgagaa
     1501 aacttttctg gtggaaatcg gcactgaaga gctgccacca aaagcactgc gcagcctggt
     1561 tgagtccttt gctgcgaatt ttactgtgga gctggataac gctggcctcg cacacggcac
     1621 cgttcaatgg tttgctgctc cgcgtcgtct ggcgctgaaa gtagctaacc tggcggaagc
     1681 gcaaccggat cgtgaaatcg aaaaacgcgg cc
//
LOCUS       DAJTYD010000286         1688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTYD010000286 DAJTYD010000000
VERSION     DAJTYD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.291"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..169
                     /locus_tag="OO585_003942"
     CDS             <1..169
                     /locus_tag="OO585_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6631347.1"
                     /translation="FSEYIAPLQDAVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAP
                     DIEWPANPVRE"
     gene            284..454
                     /locus_tag="OO585_003943"
     CDS             284..454
                     /locus_tag="OO585_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631348.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(581..1138)
                     /locus_tag="OO585_003944"
     CDS             complement(581..1138)
                     /locus_tag="OO585_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6631349.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1135..1410)
                     /locus_tag="OO585_003945"
     CDS             complement(1135..1410)
                     /locus_tag="OO585_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6631350.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1596..1688
                     /locus_tag="OO585_003946"
                     /pseudo
     CDS             1596..1688
                     /locus_tag="OO585_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          470 a          356 c          413 g          449 t
ORIGIN      
        1 attcagtgag tatattgcgc cactgcagga tgctgtagat ctggaaatcg caacggagga
       61 agaaagatcg ttgctggagg catggaataa atatcgggta ttgttgaatc gtgttgatac
      121 atcaactgca cctgatattg agtggcctgc aaatcctgtc agggagtaat cattgggatt
      181 atgccgcaga cacgtcgtat gcaggaacgt gctgcggtta gtttgtgagc tttcgatagt
      241 ggttgttatt tttgccctta tttgttccgg aggccatggt tcaatggtcc gtctgccccc
      301 tgtggtgatg tcagcaaaat cagccactgc gcgaaccaca atagcccggg aagatgctga
      361 agatcaccag gtaaagctgt cagcgcagaa actggaagaa ctgctcgcat caatggttaa
      421 ggatgaggtt gatcgcaatg atgggattta ttgacgtcag cgagagcaga aggaagaact
      481 gaataacctg aatgatttac gctcaatcag agcgatggcg attagcggca atcacgctcg
      541 ataaaattaa ccccggtgat catccggggt tttgcattca ttaaaaccgc atcaaccttt
      601 ccaccaattg ttctttacgg gcaacgatcc acccatgttg ctccagataa aatttaaacc
      661 gttccagcgt gcataccatc gcatcggcgg ggactttttc tgtgaactcg acctgaccgt
      721 gtttatcgaa gtggatcagt aatgcgcatc cattatttgg ggcgggggag ttttgtgctg
      781 caggaggacg tttgtggagt tcttcttcca ttgcgttaaa tttttcaatg taagcttctt
      841 ttagtctggc tgcttttttg ccagtaaatc ccattacgag gaacatgaag ccgtcttttg
      901 tgatcaggta gcatcgtgat tcacgttctg cgccgttgcc aatttcgaca gtctgaacat
      961 cggccgaaaa gttggctgat gtaaattcgg gagagcagtc gagtgtgtca atcttagcta
     1021 aaacgtgttt atgttgttta tcgaagtagt cggcaacagc ttgagaggtg gtaaccaggc
     1081 gaccatcttt gaaaaagact tttggtgact cgttagaaaa agttatagct gtattcatat
     1141 gtgatgactc caattaattg gatgatcacc acctctgacg ccaatcaggg aggtggtgag
     1201 acgtacaggg ttggcgtaac cggtcatcac aaaacccggc gcggatttct ccgcccctgc
     1261 acgccccacc ataatgcgaa tgtggcaatg cttaacgcaa aaaaaaccgc aaacgcggct
     1321 atgcgttgtg atgttatccg agacgccaat cccgacaacc gattttgcgg ttgtgtgagg
     1381 aatatagccc cggagaacaa aaactgtcaa cgggcaaaaa aatttccgtt atcaggaatg
     1441 tataaaatta aagcgatcga gcaaaaaatc ctttctgcaa aactaaaaaa gatttcaata
     1501 aaatcaggca gttaaaaaca cggaaaaacg gtataaaaaa gcgcctcaaa atcctgtttt
     1561 ttctttcaga tacaatgtct tattgcattt tttaaaacat gtcagtctgg tacatggata
     1621 tcgataccac cgccaattat aagctgggcg gtgcacagca acatgatagc gtacgcctcg
     1681 atccgtgg
//
LOCUS       DAJTYD010000287         1672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTYD010000287 DAJTYD010000000
VERSION     DAJTYD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.292"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(855..1328)
                     /locus_tag="OO585_003947"
                     /pseudo
     CDS             complement(855..1328)
                     /locus_tag="OO585_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1256..1622)
                     /locus_tag="OO585_003948"
                     /pseudo
     CDS             complement(1256..1622)
                     /locus_tag="OO585_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          371 a          465 c          405 g          431 t
ORIGIN      
        1 tcgtcaaaaa aaatcatctt cgtgaagttt tacgacatag tggaggagtt tgcttctttg
       61 aggtgcaggt tttcgtttgc gtggtttagc tgaacgggcc atataactac ctgaaagaca
      121 atgatattgt ctgtttttat aacggtaatc acaggccatg acaagccgca gccgtcaggc
      181 cgcctactcg gggcggttta gaacgcttat ttagtaccag atcttactta taataaaggg
      241 gcttggcagt ttggataaac tattgtcctt attccactaa tacaaacttt ttgtaaagta
      301 tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac
      361 cataaaagga agagggggcc ccaattaagt tgtcaagcat gtttactgcg caatcgcttg
      421 gtgctccgtc cctgtacccc gcattggtac tctggcgctc atggggcttc cacttgcgct
      481 tctcccttca catcggggtg acaggtccac gttccccaca agagcctgaa tcgacttcac
      541 gccgctttta tgccggatgc cgcgcaggca gtaaacatat cccacaggaa cgactccacc
      601 attgttttga catcatctat atgctttcga cacatcatca gcggttcact ttcgttcgtc
      661 tcttcgaacc atacctgatc ggctcacgtc cgaccttttc ccatctcgct caccaccaca
      721 tctcttaaat gcagcagcag tggatggttt ggtgcctgcg cctgcacgcc gacaccgagg
      781 ggccttccct catctcttgt gccgcatggc tgcacaggga ctcacgtccc caggacgcgc
      841 cttcgtggca cacagtcatc ggcatagcaa cagtaagcaa cggcaggttt ccattgccag
      901 ttttctttga cagcagtact tcggcctcgc tggatactgt gattccagta ccagcgatcc
      961 tttctggctt ttccactcag gtagcgttta ttcagatact ggtcgaactc attcagtatg
     1021 atgttcgaca gcaacggcga gatcacaccg ccctgtggca tactttcact gaccgcccgg
     1081 aagagaccaa catcaacacg gcgttttcca cagcagtctc ataaaacgtg catcactgat
     1141 cctgcggcag acggctttca tcagcagccg gtgatgtact gtgtcgaagt agctggacag
     1201 gtcaccttca ataacccggc gccccgtgtt tccccgcagt cggtgagctg taatagtacg
     1261 tgatgtctcc cgcccacttc tggttcgggc cactggcgta aaaatcctgc gtcagcagat
     1321 tttctgatac aggcaggccg tgtgcacggt aactgatcgg gctgaacttc tgggaggctt
     1381 ttgcgctcag ttcctgacgg cgcagactgg ctgccacggt ttttacgtta aagtggtaac
     1441 cagagcacac agttcatccg tcaggcgtgg ggcaccgtaa cgctggactg ggtaaaagcc
     1501 ttgcggacaa cgctgtcgca gtgctgacgg aactgctgac gtgtgtttat ccttgtccgc
     1561 cgctgacacc acgtatacca gccgctgcgg gccacccgga gtacgcggca cattgccttg
     1621 atgacggttt acgcggtcgc ctgcggcaac ggctttccgg ctgacggtag tg
//
LOCUS       DAJTYD010000288         1647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTYD010000288 DAJTYD010000000
VERSION     DAJTYD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.293"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..351)
                     /locus_tag="OO585_003949"
     CDS             complement(<1..351)
                     /locus_tag="OO585_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631351.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAAN"
     gene            382..948
                     /locus_tag="OO585_003950"
     CDS             382..948
                     /locus_tag="OO585_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6631352.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1091..>1647
                     /locus_tag="OO585_003951"
     CDS             1091..>1647
                     /locus_tag="OO585_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6631353.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWP"
BASE COUNT          440 a          399 c          433 g          375 t
ORIGIN      
        1 atttgcagca gtcgctatgc ttgaaaaacc gcggaactga tttgatgtaa ttgcagaagc
       61 cgacaggatt cctgcgcctg aaggtgtatg ccccacatga ctaactatca tcccataaca
      121 tgctgacgga aaggcaaaag gaaaatcatt aagatatcct gcatcttcac caccagaccc
      181 accaaatctc gcctgccccc actgaataat cagtgttctc cgggaacctg aaataattaa
      241 cggaatcgtt acatacccat tcaggccaat gacacccgat gcagtgccag ccagagataa
      301 ttctcccaaa ccaacgttta agaaaatgca gagataatgg ctaactggca tcatccccgg
      361 tttttattca ggggattgat catgcttatt ggctatgtac gcgtgtcaac aaatgaccag
      421 aacaccgatt tgcaacgtaa tgcactgaac tgcgcgggat gtgagcggat ttttgaggat
      481 aaaatcagtg gcactaagcc cgacagaccg gggcttaaaa aactgctcag gacactatcg
      541 gcaggagaca cgctggttgt ctggaagctg gacagactgg gacgcagtat gcggcatctt
      601 gttacgctga tagaagagtt gcgccagcgt ggtgtgaatt tccgaagcct gactgacagt
      661 attgatacca gtaccccaat gggccgtttc ttttttcacg tcatgggggc tctggctgaa
      721 atggaacgtg aactgatagt tgaacgtacc agagcagggc tggctgcagc tcgtgccaaa
      781 ggcagagtag gtggacgccg tcctaagttg accaccgaac agtgggcaca gattggacgt
      841 ttactcgagg ccggagaatc aagacagcgt attgcactga tttttgatgt gggcgtttct
      901 accatttata gaaaatttcc ggcgaatgag agcaatgaat cgccctgagt cagtattatt
      961 ttgatttttc ctgtaagcgg acaaataccg tcattttgtg tgaataacga cacaactgcg
     1021 cttagctgtt tgtcaggcac aatcacttca acatagggcg aagcctaatc caatcaggag
     1081 gttcgccact atggctcagg attaccacca cggggtgcgc gttgttgaag tcaacgaagg
     1141 cacccgatcc attaccacgg tgagcaccgc catcgtgggt atggtctgca cgggcgatga
     1201 tgccgatgca aaaatgtttc ctcttaataa acctgtgctg atcactgatg tgctgaccgc
     1261 cagcggtaaa gcgggtgagt ctggcacgct ggcccgttcg ctggatgcca tcgctgacca
     1321 ggcaaaaccc gtgaccgttg ttgtgcgtgt gccgcagggt gaaacggaag aagaaaccac
     1381 aaccaatatc attggcgcag tgaccgccga gggtaaaaaa acaggtatga aagccctgtt
     1441 atctgcccag tcacagctcg gcgttaaacc gcgcattctc ggcgtgccag gtcacgacac
     1501 gaaggcggta gctactgagt tgctgagcgt ggcgcaaagc ctgcgtggat ttgcttacct
     1561 gtcagcgtat ggttgcaaga cggtgcagga agcaatcact taccgtgaaa acttcagtca
     1621 gcgtgaagga atgctgatct ggcccga
//
LOCUS       DAJTYD010000289         1546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTYD010000289 DAJTYD010000000
VERSION     DAJTYD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.294"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(918..1394)
                     /locus_tag="OO585_003952"
     CDS             complement(918..1394)
                     /locus_tag="OO585_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6631354.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          352 a          413 c          383 g          398 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc catgattttg cccgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccgtc cgttcaccgt taaaacgcct
      241 cagaaatcgg gacaggatgt gggaatgtcg gaccgcgcac gcgcactcag acattcccca
      301 cctggtcagg gcttcacccc gacaccctgc atccgtgcgt aagtgctcaa tgtaacttcc
      361 ggttctcttc cgcttcctcg tcgattcgct cgctacgctc ggtcatcgac ttgcggcgag
      421 cgggatcacc tctcgcatgt tgtcggtgat cccgttgttg ttctttcggg gatgagtcgt
      481 cagatagccc ttcaaacacg ctcagaagct ctcagacgca cgaaacgggt ttaaatgccc
      541 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aggcgcgccg
      601 atacccctaa ccaggggctt tcaggtcgct aggaaccgcg tcagctccct gcaaagccta
      661 tttgcacggt acaggcagct attcggctgg ttggtggtcg tagaccgtcg gtgttacagc
      721 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
      781 cgtggcgacg cggtcgttct tgggatcggg gagcaaaaac acgcaaggga ttttgaggcg
      841 gcaaaagagc aatgcaggat agacaatcat ggtcaaaacg ttaacacgta gcccctgatt
      901 gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg aatatcaccg
      961 tctttgttga ccagtgtttt agtgacaaac gctgcatggt ttttcgtagt gttgtatccc
     1021 ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac ggggattcta
     1081 actaatgcat tttttgattc taagtaattg agccaccgaa taattgtgct tctgctaact
     1141 ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt aggatcgata
     1201 tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttggctatc cttcagcagc
     1261 tctcgaacgc gttcccaacc ttttggggat acctgtgtaa accttgaatt cttctttgct
     1321 tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag tcgtttttct
     1381 gaatcaataa acatgatttt ttccctctga aaatcccatg cactaacgtt ggttcaaatc
     1441 taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc caggatgcga
     1501 caacgttgtg tcaccctgga tgcgacaggt aaacaaaaaa cacact
//
LOCUS       DAJTYD010000290         1540 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTYD010000290 DAJTYD010000000
VERSION     DAJTYD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.295"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(472..798)
                     /locus_tag="OO585_003953"
     CDS             complement(472..798)
                     /locus_tag="OO585_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631355.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALGVNSWRGKSKYLDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          530 a          278 c          264 g          468 t
ORIGIN      
        1 cagtttgatt attcacaatt cattatcagc attttctgtt tctgacgaaa tcaacatgaa
       61 aagaaccata tatgataatt attataataa cggctttaat tggaatacat atattacaac
      121 gtattatata taattggtat tctgggaact atattctcaa aatacagtag aaacgaggta
      181 tgtttctggt ggaaaggaca gtgggattaa aaagtaagaa ttgataaaaa aacgccagca
      241 acacatagtg ccggcggagg gaatccccca tggagaaaat gtgatgccta gagcaccatg
      301 aatatataat taaaaatagt tagagttgtc aatactttca caaaaataat tttcgtagtt
      361 tcgtagtata gaaagatatt tttatgcatg acctacctga atttgctccg ggtagaggtt
      421 ataaataaaa attgaatcac gacaaacaca atattcatag tatggcgatg cctacgccag
      481 caagaatagc gccaataatg ttgggaatat aatagacacc agacgcacag gcatctcatc
      541 tcttaacaaa taataatcaa ggtatttact tttgcctcgc cagctgttta cgcctaaagc
      601 acacaagatt aaccattcaa taacaaatgg caatatccat agccatacga ctttaccttg
      661 taatgttcgg tatttcttta taattattct gggaaatcta tcatttattt ttaaaatcaa
      721 attatcttgt tgtttaaaaa taagttcaca tactttatta tcttcttccg ccatcaacat
      781 ctctgcatac ttaaacattt cagaacgttc ctttagcaca gaagagtaat tatatgtcca
      841 gttcccaggc aataatgctt atggatattt aactaataat ttagagaata ttttgcaata
      901 atatttggct gtattcagaa atacctgaaa aatcatacta tacagcccca gggaatggat
      961 aaagattcta acaaagcatt tcaacaatat atgcttgtta aaaatccata tcgaaatgcc
     1021 gttgcagcat taatatatgc ctcattcata aaatgtaaaa gagcaagctc gtaccagtag
     1081 gggggaaatt cattgacatg tcctgtcaat acgtacagag ccctgtcatg cttgccagca
     1141 cgtaacaatt ccgtccccag taaccagcgt gtcccctgat tattctcagg gttatatgtt
     1201 agtattttat caatgagtat aacggcatcc tgatgacgct gtaaatgaac attcgccaga
     1261 attgcagcat aaagagcgca aatgcatatg ccagatgagc gtgaatatca atgatgtcag
     1321 gagcatgtca ggcaagacgc ctgagtttgt tgacgtaatt tgtttcgttt atttcatcac
     1381 tatcgtatgc gtcaagagtt ttttcaaact catcccgaaa tgggccaagc ctggtttcag
     1441 gaaataagaa atacccctag ttattgttca cttaaaagta taatttgtaa ttcaaatcct
     1501 gagcctgtaa aacggggagt aacatattct attctgaaga
//
LOCUS       DAJTYD010000291         1512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTYD010000291 DAJTYD010000000
VERSION     DAJTYD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.296"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..238
                     /locus_tag="OO585_003954"
                     /pseudo
     CDS             1..238
                     /locus_tag="OO585_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(255..434)
                     /locus_tag="OO585_003955"
                     /pseudo
     CDS             complement(255..434)
                     /locus_tag="OO585_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            764..>1512
                     /gene="ipaH7.8"
                     /locus_tag="OO585_003956"
     CDS             764..>1512
                     /gene="ipaH7.8"
                     /locus_tag="OO585_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6631356.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSS"
BASE COUNT          480 a          327 c          240 g          465 t
ORIGIN      
        1 ccctgttgac tccgttctgt atcggcatgg tgaccagcga tgactggggc agctacgcca
       61 gagaagtgcc ggaggagaaa cacctgaccg gcaaaatctt cacgcaacgt atgaacgtaa
      121 caacctgacc ctccgccccc gcatcaagcg gctggcacgt aaaacaatct gcttctcgcg
      181 ttccgtggaa atccacgaaa aagtcatcgg ttccttcatt gaaaagcaca tattctaatg
      241 attggaagta ctacccggca aaactcattc agtgcattcc agtgattcag gatatagttg
      301 aacgccttta ccatttcagc atggtttgac aaagttttcc tctggagctt tatccagtca
      361 tacaaggact tcattcgcgg cacacatttc tcctgcctta ccgcaagccg tttttctgcc
      421 ggcctccctc atattaaatg tgtatcgttt ttttacagta attttaattt atcattctat
      481 aataggaata aatgagcttg gctaaaatat gatgtaaaac aaatatactt actcggatta
      541 catttccttg ctcactggaa tgttgaattc agtgttatta ttgactgcag tcacaataaa
      601 aaaaagaatt ccatagaaaa cctcaacaaa taaattacaa ctaacttctg ttatgtgtaa
      661 aatggaaact attaaaactt aatatcgaaa atggtaagtg aaatttgtat aaatatacaa
      721 ttttaaatat ttattctcac aaatataagg ttgccctagc attatgttct ctgtaaataa
      781 tacacactca tcagtttctt gctccccctc tattaactca aactcaacca gtaatgaaca
      841 ttatctgaga atcctgactg aatgggaaaa gaactcttct cccggggaag agcgaggcat
      901 tgcttttaac agactctccc agtgctttca gaatcaagaa gcagtattaa atttatcaga
      961 cctaaatttg acgtctcttc ccgaattacc aaagcatatt tctgctttga ttgtagaaaa
     1021 taataaatta acatcattgc caaagctgcc tgcatttctc aaagaactta atgctgataa
     1081 taacaggctt tctgtgatac cagaacttcc tgagtcatta acaactttaa gtgttcgttc
     1141 taatcaactg gaaaaccttc ctgttttgcc aaaccattta acatcattat ttgttgaaaa
     1201 taacaggcta tataatttac cggctcttcc cgaaaaattg aaatttttac atgtttatta
     1261 taacaggctg acaacattac ccgacttacc ggataaactg gaaattctct gtgctcagcg
     1321 caataatctg gttacttttc ctcaattttc tgatagaaac aatatcagac aaaaggaata
     1381 ttattttcat tttaatcaga taaccactct tccggagagt ttttcacaat tagattcaag
     1441 ttacaggatt aatatttcag ggaatccatt gtcgactcgc gttctgcaat ccctgcaaag
     1501 attaacctct tc
//
LOCUS       DAJTYD010000292         1500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTYD010000292 DAJTYD010000000
VERSION     DAJTYD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.297"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            87..608
                     /locus_tag="OO585_003957"
     CDS             87..608
                     /locus_tag="OO585_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631357.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
     gene            611..1156
                     /locus_tag="OO585_003958"
     CDS             611..1156
                     /locus_tag="OO585_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6631358.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     VITDKTSYNAIEWPVSPEGSS"
     gene            1180..>1500
                     /locus_tag="OO585_003959"
     CDS             1180..>1500
                     /locus_tag="OO585_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6631359.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFE"
BASE COUNT          418 a          325 c          394 g          363 t
ORIGIN      
        1 ggtatcggcg ttcagaacgc cctgggggga attcaattgc tttcggggat aacgatacag
       61 gtataaaaca aaacggcgac ggcattctgg atgtttatgc gaatggacag catgtatttc
      121 gtttccagaa tggtgtggcg atagcgttaa aaaatattca ggccggaaat gctaaaaaat
      181 tcacgttatc cagcgccaac aactccacga aaaatgcagc gttttatttg tggggtaatc
      241 catccaggcc tgttgttgca gagcttggtg atgattccgg ctggcatttc tacagccaga
      301 gaaataccga tggtagtatc acattcgctg taaacggaca ggtaattccg ttaaattacg
      361 gaaacttcga tgcccgctat aaacatcgaa ctgagggggt acaggatgtg cggtatggtt
      421 atgaaatgta ttacaaccct ggcagcaaca ccgtttcatg gacgtttcgc tcaccttcgg
      481 gacacgggtt gtcagggata tcgatatcgg ataccggccg taactcagcg gataacgtca
      541 acggtgtgta ttaccgaccg ctgcaaaaac tgattaatgg catctggtat aacgtagcga
      601 gtatttaaca atgttgcatt taaaaaatat tacggcaggt aatccgaaaa ccgtggaaca
      661 atatcagctg acaaaacaat atgatgtcac ctggcttttt tcgaaagacg gaaaaaactg
      721 gtatgaagag ctgaagaatt tcgccagcga cacaattaaa ataacttaca ccggagacgg
      781 tcgcgtggtg tgggtcggta aggatgtgac tggcattgaa ccccgtaacg ccagtgttat
      841 tgaagttcct gatattaccg ccaaccgccg cattaccgcg ccgggttact ggttttaccg
      901 caatgatgaa tttgtctttg actacagact caaagcggaa gatgagcgtg atgcccttct
      961 ggctcaggtc agtgcccgga caggggaatg ggaagaagac ctgctgctgg ggctaatcag
     1021 cgacgaagac cgggaaaaac tgaaagcgta ccgcatttac gcgaaatcgc tgcaggcgat
     1081 ggatttcagt gtcatcactg ataaaacctc atacaacgcc attgaatggc ccgtctctcc
     1141 ggaaggttct tcctgattta atttatcgcg agaaaaacta tgtctgtagt gatatcaggt
     1201 gcgctgactg atggcgcagg catccccatg tccggatgcc agattattct gaaatcccgt
     1261 gtaaacacct cagaagtggt gatgcgtacc agggctgatg tagtgaccgg aaataacggt
     1321 gaatattcgt ttgaggcaca ggtcggaaaa tattgcgtgt atctgaaacg ggactggcgc
     1381 gacgagtact gtgttggcga cattgctgta tacgacgact caaagcccgg cacgctgaat
     1441 gactttctga ctgcccccga tgaaggcgac ctgaagccgg atgtcgtcaa acgctttgag
//
LOCUS       DAJTYD010000293         1452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTYD010000293 DAJTYD010000000
VERSION     DAJTYD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.298"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..283
                     /locus_tag="OO585_003960"
     CDS             <1..283
                     /locus_tag="OO585_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6631360.1"
                     /translation="KDEPLTLLKNVRGLELLTFAEQDTCCGFGGTFSVKMAEISGEMV
                     KEKVGHLMEVRPEYLIGADVSCLLNISGRLQREGQKVKVMHIAEVLMSR"
     gene            294..>1452
                     /locus_tag="OO585_003961"
     CDS             294..>1452
                     /locus_tag="OO585_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6631361.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHR"
BASE COUNT          361 a          316 c          418 g          357 t
ORIGIN      
        1 gaaggacgag ccacttacgc tgctgaaaaa tgtgcgtgga ctggagctgt tgacctttgc
       61 tgaacaggat acctgctgcg gatttggcgg cacgttctcg gtcaaaatgg ccgaaatatc
      121 cggcgagatg gtgaaagaaa aggttgggca cctgatggaa gtccgccctg agtatttaat
      181 tggtgctgac gtgagttgcc tgctgaacat cagtgggcga ttacaacggg aagggcagaa
      241 agtcaaagtg atgcatattg ctgaagtgtt gatgagccgc tgaggatata aagatgtcga
      301 tcaaaaccag taatacagat tttaagactc gcatccgtca gcaaattgaa gatccgatca
      361 tgcgcaaagc ggtggcaaac gcgcagcagc gtattggggc aaatcggcaa aaaatggtcg
      421 atgaattggg gcactgggag gagtggcgcg atcgggccgc ccagatacgt gatcatgttc
      481 tgagtaatct cgacgcttat ctgtaccagc tctcagaaaa agtgacgcaa aacggcggtc
      541 acgtctggtt tgcaaaaaac aaagaagacg ctacccgcta cattttacag gttgcccaac
      601 gcaaaaatgc ccggaaggtg gtgaaatcta aatcgatggt gaccgaagag attggtgtca
      661 atcatgtgtt gcaggatgct ggcattcagg tgattgaaac cgatctgggt gaatatattc
      721 tccagctgga tcaagatccg ccatctcatg ttgtggtccc ggcaattcat aaagatcgcc
      781 atcagatccg tcgagtgcta cacgaacgtc tgggctatga ggggccggaa acgcctgaag
      841 cgatgacctt attcatccgg caaaaaatcc gcgaagattt cctcagtgct gaaataggta
      901 ttaccggctg taatttcgcg gtggcagaga caggttcggt atgcctggtg accaatgaag
      961 gtaatgcgcg aatgtgtacc acgctgccta aaacgcatat tgcagtgatg ggaatggagc
     1021 gtattgcccc cacgtttgcc gaggtagatg tattgatcac catgctggcg cgcagtgccg
     1081 ttggtgcacg tttgacggga tacaacacct ggctgacagg accgcgcgaa gcggggcacg
     1141 ttgatggtcc tgaagagttt catctggtta ttgtcgataa cgggcgttct gaggtgctgg
     1201 cctctgaatt tcgggatgtg ctgcgctgta ttcgctgcgg ggcttgtatg aatacttgtc
     1261 cggcatatcg ccatattggc ggtcatggat atggctctat ttatccaggg ccaattggtg
     1321 cggtgatttc tccgctactt ggcggctata aagattttaa agatttaccc tacgcctgct
     1381 ctttatgcac tgcttgtgac agcgtgtgtc cggtgcgtat tccgctgtca aaactgattt
     1441 tgcgtcatcg tc
//
LOCUS       DAJTYD010000294         1441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTYD010000294 DAJTYD010000000
VERSION     DAJTYD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.299"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(340..606)
                     /locus_tag="OO585_003962"
     CDS             complement(340..606)
                     /locus_tag="OO585_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631362.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(788..1186)
                     /locus_tag="OO585_003963"
                     /pseudo
     CDS             complement(788..1186)
                     /locus_tag="OO585_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          366 a          298 c          302 g          475 t
ORIGIN      
        1 aaagccgtct atgggaagga gtctgctgtt attccagaga atggtaattc agtcatatat
       61 ttctccatct atgacttgtc aataccatcc tgatatttgt gggtcagcaa tatattctac
      121 taaaaggtat ttaaggtcat tgcgtccctg cactcgaata atggtgaaga aagcatgatg
      181 aatatatcac gataacgttc agtatgtatc gaggatagat ggagttactc ctctctgaaa
      241 tagaattact gctctacttt ttctgtttcc agaaattatt agtaacaaac cctgatatat
      301 tcttttgaaa atggagctta aacgagtctg aaattaactt cagatattag aatgccgttg
      361 gctttgtttt tcaacaattc ctacttcatt tatctttgtc aaccattgca tttctgtagc
      421 agtgaatttc aacgttttcg tccggttggt agcaggtatt tgtaattcgt atatagctgc
      481 ttttttattg aaaacagtat atccatctgt agtaacagta gaagataata atacaggatt
      541 tgacacttca aggatgatat tttcttgttt ttgtggaagg caagggaata tagtttgtgt
      601 aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac attatagtcg
      661 gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc
      721 cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg
      781 ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca
      841 acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg
      901 ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc acgaaccgtt
      961 ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact
     1021 ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat
     1081 tggccgttcc ggtcctttat cgccatatcg ctgacatgac atcctgcacc gttgctttgt
     1141 cataatctcc cgtgcctgct gcccagtcta tggcttcacg atcgcaatat ggaacgtaac
     1201 gctgtcatcg ggagaatcgt caatgcggca ttcggttaac gctgatatga ctttccgtgt
     1261 caaaccggaa tctctaattt tgacgatttt ctttttaggt ttaattccct gagggcgacg
     1321 tagcagtaaa tccgacggtg aatcacgctg atattcgcgg gttggatacc gcattacaga
     1381 ggtccgtcat atgagcctgt cactttctta cgtcgcgaat cagctttgtt ccgccgtcgc
     1441 c
//
LOCUS       DAJTYD010000295         1433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTYD010000295 DAJTYD010000000
VERSION     DAJTYD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.300"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1287)
                     /locus_tag="OO585_003964"
     CDS             complement(<1..1287)
                     /locus_tag="OO585_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6631363.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQ"
BASE COUNT          332 a          401 c          383 g          317 t
ORIGIN      
        1 ttgtttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg gataaacggt
       61 gagtggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
      121 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
      181 cccttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
      241 attggcgtgc tgatcgatgg tcacatggtc cgctttagtg gcagcaaaca atagcttatc
      301 gataaccggc gaaaacaaac gccggaacaa ggtacgctgc ccgtagtgga aactttgcat
      361 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
      421 caggcaatca accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
      481 cttcaccacc ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
      541 cttatcggcc tgcgccagtt tggactcgcc ccaggtatcg acatccggcc acgggaagaa
      601 ttgcagcgcg ggcgcacctg ccatatctcc tggcaagaca aagcgccctg gctgaataaa
      661 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc
      721 cagccggttt tcgtcggcag gtgctagcgg gtccagccct tcgcacatca ttcgccattt
      781 gaccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgtg accagcttaa
      841 atagtcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatctc
      901 cagatacagc gtggaggtgt ccttgaagtg gcgtagcagc gaatcgttcg atttaaaacg
      961 tagcgccagg cggatttcac tgacgccgcg cgttggcgtc ggccaggcgg gaggatcgcc
     1021 atataactgc gccagtcctt catcatatgt gaagcgcgga atgccaaagt cacgctgagg
     1081 aatgcgtttt acgcccagca ggcgctcttc acgcaccgcg cttaacagcg gcaagcgtgc
     1141 tccggcgtga atattgagca actgattgac catagcagtg ataaacgctg ttttgccgct
     1201 gcggcttagt ccggttacgg cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     1261 attaagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     1321 aatcattcgt aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg
     1381 tcatcgacgg gcgaactcca taagatgtgc agcattcacg ttcggacgga gtt
//
LOCUS       DAJTYD010000296         1429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTYD010000296 DAJTYD010000000
VERSION     DAJTYD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.301"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..820
                     /gene="fliF"
                     /locus_tag="OO585_003965"
     CDS             <1..820
                     /gene="fliF"
                     /locus_tag="OO585_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6631364.1"
                     /translation="PNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAP
                     ISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVV
                     VNYKTLPDGKPLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELP
                     FWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVED
                     AVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            813..>1429
                     /gene="fliG"
                     /locus_tag="OO585_003966"
     CDS             813..>1429
                     /gene="fliG"
                     /locus_tag="OO585_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6631365.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     VATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINL"
BASE COUNT          362 a          385 c          414 g          268 t
ORIGIN      
        1 ccctaacggt gatgaatctc atgcggcgct tcgttcacgc cagcttaatg agagcgagca
       61 aagcggttcc ggttatccgg gcggcgtacc gggggcgttg tcgaatcaac cggcacctgc
      121 gaataacgcg ccaatcagca cgcctccggc aaatcaaaat aaccgccagc agcaggcgag
      181 caccaccagc aatagcgggc cgcgtagcac acagcggaat gaaaccagta actacgaagt
      241 cgatcgcacc attcgtcata ccaaaatgaa cgtgggcgat gtgcaacgtc tgtcagtcgc
      301 ggtagtggta aattacaaaa ccttgccaga cggtaaacca ttgcctctca gcaacgaaca
      361 gatgaagcaa attgaagatc tgacccgcga ggcgatgggc ttttctgaaa aacgcggtga
      421 ctcgctcaat gtcgttaact cgccgttcaa tagcagtgac gaaagcggcg gagaactgcc
      481 gttctggcaa cagcaagcgt ttatcgatca gttgctggct gccggtcgct ggttgctggt
      541 actgctggtg gcgtggctgc tgtggcggaa agcggtacgc ccgcagttaa caagtcgcgc
      601 cgaggcgatg aaagctgtac agcaacaggc gcaggcccgt gaggaagtgg aagatgcggt
      661 ggaagtccgc ctgagcaaag acgaacaact ccaacaacga cgcgctaacc aacgtctggg
      721 ggcagaagtc atgagccagc gtatccgtga aatgtctgat aacgatccgc gcgtggtggc
      781 gctggtcatt cgccagtgga taaataacga tcatgagtaa cctgacaggc accgataaaa
      841 gcgtcatcct gctgatgacc attggcgaag accgggcggc agaggtgttc aagcacctct
      901 cccagcgcga agtgcaaacc ctgagcgctg caatggcgaa cgtcacgcag atctccaaca
      961 agcagctaac cgatgtactg gcggagtttg agcaagaagc tgaacagttt gccgcactga
     1021 atatcaacgc caacgattat ctgcgttcgg tattggtcaa agctctgggt gaggaacgtg
     1081 ccgccagcct gctggaagat attctcgaaa ctcgcgatac cgccagcggt attgaaacgc
     1141 tcaactttat ggagccgcag agtgccgccg atctgattcg cgatgagcat ccgcaaatta
     1201 tcgccaccat tctggtgcat ctgaagcgcg cccaggccgc cgatattctg gcgttgttcg
     1261 atgaacgtct gcgccacgac gtgatgttgc gtgtcgccac ctttggcggc gtgcagccag
     1321 ccgcgctggc ggaactgacc gaagtactga atggcttgct cgacggtcag aatctcaagc
     1381 gcagcaaaat gggcggcgtg agaacggcag ccgaaattat caacctgat
//
LOCUS       DAJTYD010000297         1412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTYD010000297 DAJTYD010000000
VERSION     DAJTYD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.302"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1359)
                     /gene="sbcB"
                     /locus_tag="OO585_003967"
     CDS             complement(<1..1359)
                     /gene="sbcB"
                     /locus_tag="OO585_003967"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6631366.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWSGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQY"
BASE COUNT          343 a          353 c          351 g          365 t
ORIGIN      
        1 atattgttgt accagcatct gcaattcatc agcataaccc tgcaaaaact ctggcgtgaa
       61 tacctggcgg cggtgctcca gccagcgttg ctgctcggca taatccagcg tccccgggaa
      121 gttgcgtgcc cgataattga acaacagctt ttcaatccgt ttatcaacaa aagtgatatc
      181 cagtgccggt aaattacgtg gctcggtttc cagcacaatt ttcattgctg cacgatctgc
      241 gtcactgaaa aagccgttat aaagctgtgc atccacgtta tctgtaggcg taaacggttc
      301 ggcttccgcg aatatcgcca ccactttttc acgcacttgc ggattttcac gcagaatttt
      361 caggttatcg aggcaatgct gacgattaat tcccagtcgg tcggcatctt ccgggcgtag
      421 cgtattcgcc tgggccagca ccggacattt attgatgtgc accagcttaa ccggaacggc
      481 ggcgttatcg ccaagatcgg tttttgcggt atataaacgc tcgcgcaatg tgtcgctatc
      541 cagttccagt aatggcgaaa tgtctcctgc caaatccacc ataattacag cattgcgatt
      601 ttcaggatgc cacgccagcg gtgccaccca gctggtattg ccgctccatg ccccaaacat
      661 tccggaaacg tgaaccaggg ggggcatctg cggaacatca atcaacgcca tcagtttgtg
      721 tttattacga tgggtaaaga gataatcaaa caggcgtggc tgacgcgttt ttaccagttt
      781 cgccatcgca atagtggcgt acacatcagc catcgcatcg tgggcgttgc tatgttcaat
      841 accattcgct ttggttaaat gctcaaggcg aaagctcggt agaccgtcat cattttcagg
      901 ccagtttatt ccttccgggc gcagggcata acaggcacgc ataacatcca acaaatccca
      961 gcgcgagtta tcatgctgcc agctccaggc gtaaggatcg tagaaattac gataaaaaac
     1021 gttgcgtgtg acttcgtcgt cgaaacgcac attgttgtag cccagaatac aggtcttcgg
     1081 tacggtaaaa agcgagtgaa tacgggcggc aaacgcggct tcgttttctc ctttcgcccg
     1141 tgcttcctgc ggggtaatac cggtaattaa tactgctcca ggctggggta aatagtcatc
     1201 cgcgggcttg cagtaaaaga cttcgggttc gccgatgaca ttgaattcgc tatcggtgcg
     1261 aatggctgcg aactgtgcag ggcgatctaa cgcggggtgc gtgccaaagg tttcgtaatc
     1321 gtgaaagagg aaggtaggtt gtttatctgt gtctgtcatt gtctagcatt gtccactgta
     1381 gttaattaac tgtctgttat aactcgaaaa ac
//
LOCUS       DAJTYD010000298         1409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTYD010000298 DAJTYD010000000
VERSION     DAJTYD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.303"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            60..>1409
                     /gene="cysJ"
                     /locus_tag="OO585_003968"
     CDS             60..>1409
                     /gene="cysJ"
                     /locus_tag="OO585_003968"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6631367.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     "
BASE COUNT          321 a          403 c          397 g          288 t
ORIGIN      
        1 tgttttatgg gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca
       61 tgacgacaca ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc
      121 ttcaggcggc cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct
      181 ggggcgtact caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa
      241 tgccgggtat aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag
      301 cattacgcga tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact
      361 ataaattcaa acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg
      421 aaggggaacc gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc
      481 caaagctgga aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt
      541 tctgccagtc cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc
      601 tcgaccgtgt cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg
      661 tggttgatgc gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg
      721 gcgcggtaaa tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc
      781 tctctgttaa ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa
      841 ttgacttagg tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc
      901 agaacgatcc ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac
      961 ctgtcaccgt cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac
     1021 tgaccgtcaa caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac
     1081 tgctgccgct ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg
     1141 tcgacatggt gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc
     1201 gcccgctgac cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag
     1261 tacacgtcac cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg
     1321 cctccagctt cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac
     1381 ataacgataa ctttcgcctg cccgctaac
//
LOCUS       DAJTYD010000299         1397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTYD010000299 DAJTYD010000000
VERSION     DAJTYD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.304"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..180)
                     /locus_tag="OO585_003969"
     CDS             complement(<1..180)
                     /locus_tag="OO585_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6631368.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQ"
     gene            complement(181..>1397)
                     /locus_tag="OO585_003970"
     CDS             complement(181..>1397)
                     /locus_tag="OO585_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6631369.1"
                     /translation="TVAENIYANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMV
                     GNMSMAHRQLVEIAKALSYAADVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYI
                     SHRMDEIMRISDDISVIRDGEYIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPD
                     ENVPAKLEVKNLSHPSLFKEISFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTV
                     LIDGKEVRITNPKQAIDHGIAFVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLIN
                     HRQEEAKTLQSIARMNTKVSSVHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRG
                     VDVGAKFEIYNVIRQLAAAGTAIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLT
                     QTQVMTAATGVR"
BASE COUNT          368 a          350 c          272 g          407 t
ORIGIN      
        1 ctgaagaata atattagtaa tgttgttggc ggtaaaaaaa ctgtcattaa taaaactgaa
       61 cagaatgatt aacaccacca ttccactgag gataccgcca tattgcctga agactttttt
      121 aaaattattt gcaatgggaa tttccgaagg tttcggactg gcaagagttt tctctgtcat
      181 ttaacgtact cctgttgcag cggtcattac ctgtgtctgc gttaaatttt cacatgaata
      241 aatatcggca atcgttttat tacgcattac caccagacga tcgctaagtg ccatgacttc
      301 tggcagttca gaggacacca gaataattgc tgtccctgcc gcagccagtt gacgtatcac
      361 gttatagatt tcaaattttg cgccgacatc cactccgcgc gttggctcat cgagcaataa
      421 aatacgcgga gttttctcca gccacttcga taacacgatt ttctgttgat taccgccgct
      481 cagcgctccc actgcctgat gtacagaact cactttagtg ttcattctgg caattgattg
      541 cagcgtcttc gcctcttctt gtcggtgatt gatgagtccc atccgcgaag cgttatattg
      601 aaacgccaca tggtgcgtgt tcatattgac gtcgtgcatc agcactaacc cttcttcctt
      661 gcggttttcg gtcacaaaag caatgccatg atcaatagcc tgtttaggat tagtgatcct
      721 gacttcctta ccatcaatca acacagtccc ctgatacgaa acgaggccaa agagcgcctt
      781 catcacatcg gaacgcccgg cccccaccag gccaaaaaat ccgaggacct cccccggacg
      841 cacagcgaaa ctgatctctt taaaaagcga aggatgggaa agatttttta cttccagttt
      901 tgcaggaaca ttttcatccg gtatttcccc aagacgtgct ggccaaatat ttttcatttc
      961 ccgaccaacc atttgggcaa tcaaatgctg gatatcactg tttttctttt catgcgtggc
     1021 gatatactca ccgtcgcgga tcaccgaaat atcatctgaa atccgcataa tttcatccat
     1081 gcgatgagag atataaataa ccgcacagcc tcgctgcttt aatttttcaa taatgttaaa
     1141 cagaatctcc gcttcattat cacttagcga agatgtcggt tcatccataa tcacgacatc
     1201 cgctgcatag cttaaggctt tagcaatttc taccagttgc ctatgtgcca tcgacatatt
     1261 gccgaccatt tctagtgggt ctagtggtat gccccgttcc gcgaggagat tctggcaatc
     1321 tgcgagcatt tttttatcgt taataatgcc aaaacgtcgc ggttcattat tggcataaat
     1381 attttccgcc acggtca
//
LOCUS       DAJTYD010000300         1379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTYD010000300 DAJTYD010000000
VERSION     DAJTYD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.305"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1379
                     /gene="rhsC"
                     /locus_tag="OO585_003971"
     CDS             <1..>1379
                     /gene="rhsC"
                     /locus_tag="OO585_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HCR6631370.1"
                     /translation="TDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYT
                     EYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYD
                     DKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRRE
                     VLHTQGEAGLKRVVKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITR
                     ITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNP
                     HSDLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQ
                     YRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQG
                     ELTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIR
                     SEDEGLVTH"
BASE COUNT          326 a          407 c          426 g          220 t
ORIGIN      
        1 gacggatggt gccgggcgtc acttccaact ggtactgacc acgcaggcgc agcgggcaga
       61 agaagcccgg cagcaggcca tttccggcgg gacggaaccg tccgcttttc ctgataccct
      121 gccgggttac accgaatatg gccgggacaa cggcatccgt ctgtctgccg tgtggctgac
      181 gcacgacccg gaatacccgg agaatttacc tgccgcgccg ctggtgcgct atggctggac
      241 gccgcgcggc gaactggcgg cagtgtatga ccgcagcaat acacaggtgc gtagctttac
      301 ttacgatgat aaataccggg gccggatggt ggcgcaccgt cacacgggcc ggccggaaat
      361 ccgttaccgt tacgacagcg acgggcgggt gacagaacag cttaacccgg caggcttaag
      421 ctacacgtat cagtatgaga aggaccgcat caccatcacc gacagcctga accgccgtga
      481 agtgctgcac acgcagggtg aagccgggct gaagcgggtg gtgaaaaagg aacacgcgga
      541 cggcagcgtc acgcagagtc agtttgacgc cgtgggcagg ctcaggacac agacggatgc
      601 cgcaggccgg acaacagaat acagcccgga tgtggtgacg ggcctcatca cgcgcatcac
      661 cacgccggat ggcagggcat cggcgtttta ctataaccac cacagccagt taacgtcagc
      721 caccgggcct gacgggctgg aaatacgccg ggaatatgat gaatggggcc gtctgattca
      781 ggaaactgcc cctgacggcg atatcatccg ctaccgttat gataatccac acagtgactt
      841 accctgcgca acggaagatg ccaccggtag ccggaaaacc atgacgtgga gccgttacgg
      901 tcagttgctg agcttcaccg actgttccgg ttatgtaacc cgttatgacc atgaccgctt
      961 cgggcagatg acggcggtgc accgcgagga agggctgagt cagtaccgcg catacgacag
     1021 ccgtggacag ttaattgccg tgaaagacac gcagggccat gaaacgcggt atgaatacaa
     1081 cgccgccggt gacctgaccg ccgtcattgc cccggacggc agcagaaacg ggacacagta
     1141 cgatgcgtgg ggaaaggcca tccgtaccac gcagggcgag ctgacgcgca gtatggaata
     1201 cgatgctgca ggacgggtca tccgcctgac cagtgaaaac ggcagccaca ccaccttccg
     1261 ttacgatgta ctcgaccggc tgatacagga aaccggcttt gacggccgca cacagcgtta
     1321 tcaccacgac ctgaccggca aacttatccg cagcgaggat gaggggctgg tcacccact
//
LOCUS       DAJTYD010000301         1377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTYD010000301 DAJTYD010000000
VERSION     DAJTYD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.306"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1377
                     /gene="yicI"
                     /locus_tag="OO585_003972"
     CDS             <1..>1377
                     /gene="yicI"
                     /locus_tag="OO585_003972"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6631371.1"
                     /translation="EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPA
                     LPPAWSFGLWLTTSFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEW
                     DPLTFPDPEGMIRRLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWD
                     KWQPGLAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKM
                     HNHYAHIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESL
                     RGGLSIGLSGFGFWSHYIGSFENTAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYD
                     DESCDVVRFFTQLKCRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYML
                     GDNVMVAPVFTEAGDVQFYLPEGRWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTL
                     LALGNNDQR"
BASE COUNT          305 a          346 c          385 g          341 t
ORIGIN      
        1 gaagtgggat cggagaaagt ctccaaagtg cagttcagcg ttgagagcga atatctcgaa
       61 tactttgtta tcgacggtcc gacgccgaaa gcggtacttg atcgttatac ccgttttact
      121 ggtcgtccgg cgctgccgcc cgcatggtcc ttcggcctgt ggctaaccac ttcatttacc
      181 accaactacg acgaagcgac ggtaaacagc tttatcgatg gtatggcgga acgcaatctg
      241 ccgctgcatg ttttccactt tgactgtttc tggatgaaag ccttccagtg gtgcgatttt
      301 gagtgggacc cgctgacttt cccggacccg gaagggatga tccgccgcct gaaagcgaaa
      361 gggctgaaaa tctgcgtctg gattaacccc tatatcggtc aaaaatcccc cgtctttaaa
      421 gagttacaag agaaaggcta tttactcaaa cgcccggacg gttcgctatg gcagtgggat
      481 aaatggcagc caggtctggc gatttatgac tttaccaatc cggatgcctg caaatggtac
      541 gccgacaaac tgaaaggtct ggtcgcgatg ggcgttgatt gctttaagac cgactttggc
      601 gaacgtatcc caactgatgt tcagtggttt gacggttccg atccgcagaa aatgcataac
      661 cattatgcgc acatctacaa cgaactggtg tggaacgtgc tcaaggacac cgttggtgag
      721 gaagaagctg tcttgtttgc ccgctcggcc tccgtcggtg cgcagaaatt cccggtacac
      781 tggggtggcg attgttacgc taactacgaa tcaatggcgg aaagcctgcg cggtggtttg
      841 tctattggcc tttcaggttt tggcttctgg agccactata tcggcagctt tgaaaatacc
      901 gctccggcgc acgtttacaa atgctggtgc gcgtttggtt tgctctccag ccatagccgt
      961 ttacacggta gcaaatctta tcgtgtgccg tgggcctacg atgatgagtc ctgtgatgtg
     1021 gtgcgcttct tcacgcaact gaaatgccgc atgatgccgt atctgtatcg tgaagctgcg
     1081 cgtgcaaacg cgcggggtac gccgatgatg cgggccatga tgatggagtt cccggacgat
     1141 ccggcttgtg attaccttga ccgtcaatac atgttaggcg acaacgtgat ggttgcgccg
     1201 gtgttcactg aagcgggcga tgtgcagttc tacctgccgg aaggtcgctg gacacacctg
     1261 tggcacaacg atgagctcga cggtcgttgc tggcataaac agcagcacag cttcctgagt
     1321 ctgcccattt atgtgcgtga taacacccta ctggcgctgg gcaacaacga tcaacgt
//
LOCUS       DAJTYD010000302         1361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTYD010000302 DAJTYD010000000
VERSION     DAJTYD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.307"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..267)
                     /gene="mddA"
                     /locus_tag="OO585_003973"
     CDS             complement(<1..267)
                     /gene="mddA"
                     /locus_tag="OO585_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6631372.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHP"
     gene            448..>1361
                     /gene="curA"
                     /locus_tag="OO585_003974"
     CDS             448..>1361
                     /gene="curA"
                     /locus_tag="OO585_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6631373.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVK"
BASE COUNT          334 a          309 c          394 g          324 t
ORIGIN      
        1 gggatggaca taaaccgaat gttccacggt atggcgaaaa ccatcgaaac tacgccagtc
       61 gccaaacgag gcatatcccg tcactacgcc atcttcttca cttaccagca ccggataacc
      121 tgctatggtc cgcgcttcaa accaggcaat gcggttatct gcatcaaccg tttggtcatt
      181 ccagatagcc gccgtataca acacggcgtg gttataaatt tccgcaatgg cagcacaatc
      241 ggctttgcgg gcaaaacgga tggacatgtc tggcctcgat aaaataatga tgatgatgtt
      301 atcaatacta tcgaaaacag cccgaagcaa cggattcctc gatgaaatta ttgcgtgttg
      361 cagaggggat gtgacggctg caaacaaaac caatcccgtg agcctgctac tattgatgga
      421 ttcatgccaa aagcacagga gagcattatg gggcaacaaa agcagcgtaa tcgacgttgg
      481 gttctggcct cgcgtccaca tggcgcaccg gttccggaga atttccgtct tgaagaagat
      541 gatgtcgcca caccgggtga aggacaggtg ttactgcgca cagtttattt gtccctagac
      601 ccgtatatgc gtggacgtat gagcgatgag ccatcttatt caccgcctgt tgatattggc
      661 ggcgtgatgg tcggcggcac ggtgagccat gttgtggagt cgaatcatcc tgattatcag
      721 cctggcgact gggtgctggg ctacagtgga tggcaggact atgacatatc cagtggtgat
      781 gatctggtga aacttggcga tcatccgcaa aatccatcgt ggtcgctggg tgtgctgggg
      841 atgccaggct ttaccgctta tatggggctg ctggatatcg gtcagcctaa agagggcgaa
      901 acgttggtgg tagctgcggc gacaggtcct gtgggagcaa cggtggggca aatcggcaaa
      961 cttaaaggtt gcagggtggt ggggggggcc ggtggcgcgg aaaaatgccg ccatgctatc
     1021 gaggtgctgg gcttcgatgt ttgtcttgat caccacgcgg gtgattttgc cgaacaactg
     1081 gtgaaagcgt gcccaaaagg tattgatatc tattatgaaa acgtgggcgg taaggtattt
     1141 gatgcagtgc taccattact taatacatct gcgcgcattc ccgtttgtgg cttagtgagc
     1201 agctataacg ctacagagct accacccggt ccggatcgtt tacccctgtt gatggcaacg
     1261 gtgctgaaaa aacgtattcg cttgcaaggg tttattatcg ctcaggatta tggtcaccgc
     1321 atccatgagt ttcagaagga gatggggcaa tgggtgaaag a
//
LOCUS       DAJTYD010000303         1276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTYD010000303 DAJTYD010000000
VERSION     DAJTYD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.308"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..303)
                     /gene="rraB"
                     /locus_tag="OO585_003975"
     CDS             complement(<1..303)
                     /gene="rraB"
                     /locus_tag="OO585_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6631374.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLA"
     gene            465..>1276
                     /gene="argF"
                     /locus_tag="OO585_003976"
     CDS             465..>1276
                     /gene="argF"
                     /locus_tag="OO585_003976"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6631375.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVK"
BASE COUNT          322 a          313 c          321 g          320 t
ORIGIN      
        1 cgccaatgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
       61 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
      121 ggtcacttcg taaccgagtt taaacgcttc aactgctgct ttttccaggg tttccagatc
      181 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
      241 taattcttca ataatcaaac gagtttcttc acgctgttct tccagttgtt ccgggtttgc
      301 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacatacc actgacattg
      361 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat catccatata
      421 aattgaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
      481 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
      541 tagccgcgaa gctgaaagcc gataagaaaa gcggcaaaga agaagccaaa ctcactggta
      601 aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct ttcgaagttg
      661 ccgcatatga ccagggtgct cgcgttactt atctcggccc aagcggcagt cagattggtc
      721 ataaagagtc gattaaagac actgcccgcg tgctgggtcg catgtatgac ggtattcagt
      781 atcgcggcta tggtcaggag attgccgaaa cactggcgga atacgctggc gtgccggtat
      841 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
      901 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
      961 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
     1021 tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc cgcgccttgg
     1081 cacagcaaaa tggcgggaat attacgctga cggaagatgt agccaaagga gttgaaggtg
     1141 ctgactttat ctataccgat gtatgggtgt cgatggggga agcaaaagag aaatgggcag
     1201 aacggattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
     1261 acccggaggt caaatt
//
LOCUS       DAJTYD010000304         1274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTYD010000304 DAJTYD010000000
VERSION     DAJTYD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.309"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(169..598)
                     /locus_tag="OO585_003977"
                     /pseudo
     CDS             complement(169..598)
                     /locus_tag="OO585_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(646..831)
                     /locus_tag="OO585_003978"
     CDS             complement(646..831)
                     /locus_tag="OO585_003978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631376.1"
                     /translation="MDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADGMY
                     CPECGTVHWPDGVIPPF"
     gene            994..>1274
                     /locus_tag="OO585_003979"
     CDS             994..>1274
                     /locus_tag="OO585_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6631377.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRRP"
BASE COUNT          303 a          390 c          264 g          317 t
ORIGIN      
        1 ccaaaagatt aaaaaagcac cgaagaatgc gcgtttattc aacacaacct gatcatcaag
       61 taatcggtat agacacttaa tattgcttac attgattgat tcaatgtatc tctgatggtt
      121 ttttgctccg ttcccctcaa catggacgca acttcccctc tgcgactctc agccgcacca
      181 ccgtattcga gccagcggct catgcatcat aacctgctct gccagacggt aacccctctt
      241 cagccccgta aaccgcatct gacctccgca cagctcgcac ttcagcgggt caaccttcag
      301 tcactgctga tacatccctc tccttgtgat ttgcctcgcc gtttttctca ctgtctccgt
      361 tatggcgtac accacatctt ccagtaatcg ccgcttcacc ggactcagga ccccgtatca
      421 ccatccggaa ccccttatcc gccacatgcc aggagaacct ttccataaac tcgtcgccac
      481 tcatcacaag gttttcttct cgttttgtcc ggtggctgtt gtaacgcagg ccaatttcat
      541 cctgaccggc ataatgttcc aggcggctca tctgcaccgg ctgcttctcc agccagttaa
      601 gccatcggtt tccctgctga aaaatatcgg caaaacgggg aagcatcaga aaggcggaat
      661 aacgccatcc ggccagtgaa ccgtaccgca ttcagggcaa tacatgccgt cggcgttgat
      721 accggcagga attgtcgcaa attcccgctc cgtgcagcgg gcaatttccg gatacccttc
      781 gtcatcaaca cgtacaaacc agaagaccag ttttttgttt ccggcatcca caaagaacgg
      841 aatattcagg tctgcgcaac actcaacggc atcgtcaaaa ttatcaaagc gcagaacctc
      901 agcttcttcg taaccggctc atttaaaccg tctggtctgt ttcctccggc tccacaaaaa
      961 taatgtccat cattttgaag tggacactat cgtatggaat accggacctg gatcactgaa
     1021 gctttacgcc ttcatttcga agaacattta ccacgggttg tggccggacg tcgcctgggc
     1081 gtaccaaaat caactgtctg tggtatgttc gtgcgcttcc gcaacgctgg cctttcatgg
     1141 cctctgcccg caggtatgtc ggagcaggaa cttgatgccc ttctttacgg aagtgcctcc
     1201 acagtacctg tcgtacttac tgaaagcacc gttatgccca aactcccggt ggtaaaaaaa
     1261 cgtccccggc ggcc
//
LOCUS       DAJTYD010000305         1255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTYD010000305 DAJTYD010000000
VERSION     DAJTYD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.310"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(169..375)
                     /locus_tag="OO585_003980"
     CDS             complement(169..375)
                     /locus_tag="OO585_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6631378.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            687..776
                     /gene="kdpF"
                     /locus_tag="OO585_003981"
     CDS             687..776
                     /gene="kdpF"
                     /locus_tag="OO585_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6631379.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            776..>1255
                     /locus_tag="OO585_003982"
     CDS             776..>1255
                     /locus_tag="OO585_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6631380.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     "
BASE COUNT          298 a          285 c          282 g          390 t
ORIGIN      
        1 aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatattt ttcctgaata
       61 tatttcagct gggtaatacc atatctaaat atttacctga acatcttcct aataaaaaaa
      121 ccgccagcat ctccactagc ggttttcaat gtattcaaaa ctcggcaatc agtaaaaatc
      181 accggttgcc ttttcggcct gttccatcca aaccggttta tcgctggttt tcgaccagac
      241 gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga aaggcagcgc
      301 cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag ataagccatg caggatattc
      361 tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaata gcaccggaaa
      421 aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta ctactcatcc
      481 gaccacttat ttttgcttat tgatggttta tttacattca ctttgtaatt aagttgcaca
      541 aaagttaaat taatgctaaa cattagttaa accatggctt ttgccatttt tatacttttt
      601 tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca gaattctact cttccggtat
      661 cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg
      721 tgtttttatt actgggttat ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc
      781 tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc
      841 tttaggcagc gggctggcgc ggctgattaa tgacattcct cttccaggta cagcgggcgt
      901 tgaacgtata ctctttcgtt tacctggcgt ctctgaccat gagatgaact ggaagcaata
      961 tctttgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt ttatgttgct
     1021 cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc
     1081 gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata gcggtgaaac
     1141 cacgttgagc tatttcagcc agatggcagg cttaacggta caaaactttc tttctgccgc
     1201 cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt actcgccaga gcatg
//
LOCUS       DAJTYD010000306         1251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTYD010000306 DAJTYD010000000
VERSION     DAJTYD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.311"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            220..1167
                     /locus_tag="OO585_003983"
     CDS             220..1167
                     /locus_tag="OO585_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6631381.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          341 a          241 c          300 g          369 t
ORIGIN      
        1 aggtttagca ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct
       61 tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg
      121 tgcactcggc attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc
      181 catcttttgg ggtggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc
      241 gtccccagct tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga gaataatttt
      301 aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga
      361 gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg
      421 ctgcctggtg tgaaaataat atcgagattc gggacgggga tagataatat tgatcttcgt
      481 gcagcacaac aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc
      541 gcggaattta tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat
      601 gcgatgcaaa atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt
      661 ggcatggttg gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat
      721 gttgagcttt tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt
      781 cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct
      841 ttttctcctg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
      901 gctctaatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg
      961 attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa
     1021 tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc
     1081 gttgagtcat accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca
     1141 ggaagggaga taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa
     1201 ccgcaatttg tggaacacat tgcggtttat cagcattcta agtattggtt g
//
LOCUS       DAJTYD010000307         1214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTYD010000307 DAJTYD010000000
VERSION     DAJTYD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.312"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(194..499)
                     /locus_tag="OO585_003984"
     CDS             complement(194..499)
                     /locus_tag="OO585_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6631382.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            607..>1214
                     /locus_tag="OO585_003985"
     CDS             607..>1214
                     /locus_tag="OO585_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6631383.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYD"
BASE COUNT          339 a          240 c          308 g          327 t
ORIGIN      
        1 actccctgtt ctttatcgtt atataaatgt ttatgtattg aatatttagc gcgctaacaa
       61 tagagggggt ctacccattt tgggttaaga attattaatc cacatcaata gaagggtatg
      121 agtaataagg tgggattatg ttgtatgttc aaatcgccgg atgtgtcata tccggcgttc
      181 agtcgataat gtattactgc ggttcggcag gcgcgccatc ctgggtagac tgcgcgggag
      241 cagagacgtt agcgctggtg gtgcgggtgt agagaatttt atgcgtatca ttagcacaat
      301 gcccgacgac ctgggaatca ggctgatcaa cctggtcatt gggtacaata cttaatgtga
      361 agctgctttc gggtacgcca ttattgataa tgcgctgtga tatatcgctc tgtatgcgct
      421 cacaggatcc cggcgcggcg agtaccgcgg gtgaggcgag ggcgagcaga agcgcggcac
      481 agcaggttga gagtttcatc ataagctcct tacgcgaaga taacttcttt aagcatagca
      541 tttaacgtgt aaagtactgt atttgctact atgattgaga atcatctcta ctctctggtg
      601 actgttgtga aatacaaatt actaccatgc ttactcgcga tattcctcac aggatgtgac
      661 cgcacagagg taacactttc atttacccct gagatggcca gtttctctaa tgaattcgat
      721 tttgatccgc tgcgtggtcc ggtaaaagat ttcactcaga cattaatgga tgagcaaggt
      781 gaagtgacga aacgtgtttc tgggactttg tcggaagaag gctgttttga ttcactcgaa
      841 ttactggatc tggaaaataa taccgtggtc gctctggtac tggacgccaa ttattaccgt
      901 gatgccgaga cgctggagaa gagagtacgt ttacagggaa aatgccagct agcagaatta
      961 ccttctgccg gggtgagttg ggaaaccgat gataatggct tcgtgattaa agccagcagc
     1021 aaacaaatgc agatggaata tcgctatgat gatcagggtt atccactggg taaaaccacg
     1081 aaaagtaacg acaaaacatt atctgtcagc gccacgccat caacggaccc gatcaaaaaa
     1141 ttagattaca cagcggttac tttactgaat aatcaacgag ttggtaatgt aaaacagagc
     1201 tgtgaatatg acag
//
LOCUS       DAJTYD010000308         1199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTYD010000308 DAJTYD010000000
VERSION     DAJTYD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.313"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..971)
                     /locus_tag="OO585_003986"
     CDS             complement(<1..971)
                     /locus_tag="OO585_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6631384.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTAFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLA"
     gene            979..>1199
                     /gene="tnpA"
                     /locus_tag="OO585_003987"
     CDS             979..>1199
                     /gene="tnpA"
                     /locus_tag="OO585_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6631385.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLL"
BASE COUNT          270 a          301 c          285 g          343 t
ORIGIN      
        1 gcaagcacct gaccgccact ccagagctgc ttgtactcaa gctggcggcg catttcatac
       61 cagccctgat ccagtatcga acggtttaaa cctgattttg cccggacatt gcgccccggc
      121 tggctgaccg tacccgctgc tgactttgac atgttgctga ccttcaaatc ctcaatgact
      181 atcattgcgt ggtttttgct gacggtcgtt gtgactttgt gaaggtagtc cctgcggata
      241 tttgcgatac aggaatgcag tcgctgtatt ttgcgtttct gcttctgcca gttgttgctg
      301 aatttgacct tgcggcttaa ctgtcgctga agtctcgcca gcttcttctg gtttttctga
      361 aaactgttta caggctcaaa ggctgtgcca tctgacagcg tggcgagttt agccacgcca
      421 gcatccagcc cgaccattga tgctgaaggg tgaaccggag ttgatacttc actttctgtc
      481 tgaatactga tgtaccactt accgcaggac tggctgacag tgacattttt cacaacaccc
      541 gtgacctgac ggctgttccg gtagcgcatc cagcccagtt tcggcagaaa aatacggctg
      601 ttttcctgat cgagcttaac gccctgcggg tagcggaata catcattctg tccccgcttt
      661 ttgaatcggg gaaaagcagc ccgattctgg aagaagtttt tgtaagcccg ctcaaggtct
      721 ttcagtgact gttgcaatgg ctgtgacggg gcatctttaa gccattgcgt ttcagtggca
      781 tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt attaccgacc
      841 tcatgattct cattctgacg tgccagagca cgattgaaaa cgaaacgaca tgcgcctgcg
      901 aagcgcctca tttcacgctc ctgttgacca ccgggtctta actggaattt aaatgcttgt
      961 agtcgtttca caggccaaat gatactttgg tctgtgaaaa aagaaaccga tatccggcgt
     1021 ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccaa ataccgacgt
     1081 cagatttttg accatgacgc gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct
     1141 gattttgaag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca tttgttaat
//
LOCUS       DAJTYD010000309         1199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTYD010000309 DAJTYD010000000
VERSION     DAJTYD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.314"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO585_003988"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO585_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6631386.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          215 c          259 g          384 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagcctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DAJTYD010000310         1173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTYD010000310 DAJTYD010000000
VERSION     DAJTYD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.315"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            197..>1173
                     /locus_tag="OO585_003989"
     CDS             197..>1173
                     /locus_tag="OO585_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6631387.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT          316 a          310 c          255 g          292 t
ORIGIN      
        1 attcatatca acggacctct acggaggttc gtttttcgtt tcagaacgcc acgatttaag
       61 cgttctgcct acaaatcaat tctaccgaac tcaaccagat tccccccaca tcaaccccat
      121 tgtgtgggta taattgcggg cataccccag ttcgacagaa tttgtaccct ctttaacgca
      181 ttcagaaggc tgaactatgg cactgactga cgcaaaaatc cgggctgcaa agcccactga
      241 caaggcttat aaactcactg acgggactgg catgttcctg ctggtacatc ctaatggctc
      301 ccgttactgg cgtctccgtt atcgtattct gggtaaggag aagactctgg cacttggtgt
      361 gtatccagaa gtttctctct ccgaagctcg tacaaaacgg gatgaggccc gaaaactgat
      421 ttcggagggg gttgaccctt gcgaacagaa aagagctaaa aaagtagtcc ctgatttaca
      481 gctctctttt gaacatattg cgcgacgctg gcatgccagt aataaacaat gggcacaatc
      541 acacagcgat aaagtactca aaagcctcga gactcacgtt ttccccttta tcggcaaccg
      601 ggatatcaca acactcaata ccccggatct gcttatccct gttcgtgctg cagaagctaa
      661 acaaatttat gaaatcgcca gtcgtctgca gcaaagaata tctgccgtaa tgcgttatgc
      721 cgtacagtct ggcatcatca gatataatcc tgctctggat atggctggcg cattgactac
      781 ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc ctgcctgaac ttttgtcgcg
      841 tattggcagt tacaaagggc aaactgtcac ccagcttgcc gttatgctga atttactggt
      901 ttttattcgt tccagtgaac tcagatacgc ccgctggtct gaaattgata ttgacaatgc
      961 catgtggact attccagccg aacgcgaacc cctgccaggc gtaaaattct cacaccgggg
     1021 ctccaagatg cgaacaccac atcttgtgcc actcagcaaa caggctgtag ccatactgac
     1081 agaactacag acatgggctg gtgaaaatgg tctgatcttt acgggagcac atgacccgcg
     1141 taaaccaatc agtgaaaatg taatgaacgc atc
//
LOCUS       DAJTYD010000311         1169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTYD010000311 DAJTYD010000000
VERSION     DAJTYD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.316"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..226)
                     /locus_tag="OO585_003990"
     CDS             complement(<1..226)
                     /locus_tag="OO585_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR6631388.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRN"
     gene            complement(353..1084)
                     /gene="rlmB"
                     /locus_tag="OO585_003991"
     CDS             complement(353..1084)
                     /gene="rlmB"
                     /locus_tag="OO585_003991"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6631389.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT          301 a          293 c          260 g          315 t
ORIGIN      
        1 gatttcttaa taagaaggta ttaaattcat ctggattgct gatactactt accggacaaa
       61 taaaggtttc gataatcatt tggcgggaag taaaaagaat attgatttgc aaatcgccat
      121 aatcattcat tttaataatt aatgcgttct cgctatttgt tacatcaata ttttgttcag
      181 gtacagtttg cagcgccgtc gccagcgcca acagattcca tgtcatatat tattccatat
      241 agatttagtt taaatattaa taaaatgaat atttgcagta cgtaattatc ttaccagcta
      301 tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc
      361 gctgacgcac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agagaagaaa
      421 cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca
      481 taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga
      541 gtgtatgatc cgcctcacct gccgtaccga cgatccagat attctcttcc tgcaacatac
      601 gcatggtacg caccaggtta gtcacccgaa tcagcggaac gctttctgcc gcgccacagg
      661 ccactttttt cgccgtggcg ttgagctggg cggagcgatc tttcggcaca atcaccgcat
      721 gaacaccagc ggcatctgcg ctacgcaggc acgcgccgag gttatgcgga tcggttacac
      781 cgtcaaggat cagcaggaac ggctgatcga gcgaagcgat cagatccggc aaatcatttt
      841 cctggtactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc
      901 ttttctcgtc gagatattgg cggtttgcca gctggataac cacgccctgg gcttcgaggg
      961 cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa
     1021 aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc
     1081 tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg
     1141 atctctgcaa tatattgaat ttgcgtgat
//
LOCUS       DAJTYD010000312         1167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTYD010000312 DAJTYD010000000
VERSION     DAJTYD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.317"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1167
                     /locus_tag="OO585_003992"
     CDS             <1..>1167
                     /locus_tag="OO585_003992"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6631390.1"
                     /translation="MFLPDRFVKGTCPKCKSPDQYGDNCEVCGATYSPTELIEPKSVV
                     SGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANKMQEWFESGLQQWDISRDA
                     PYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSVSFDEYWKKDSTAELYHFI
                     GKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKASTWLNHFD
                     ADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVNLASRNAGFINKRFDGVLA
                     SELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALADLANRYVDEQAPWVVAKQE
                     SRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLNTELTWDGIQQPLLG"
BASE COUNT          273 a          309 c          316 g          269 t
ORIGIN      
        1 catgttcctg ccggaccgtt ttgtgaaagg cacctgcccg aaatgtaaat ccccggatca
       61 atacggcgat aactgcgaag tctgcggcgc gacctacagc ccgactgaac tgatcgagcc
      121 gaaatcggtg gtttctggcg ctaccccggt aatgcgtgat tctgaacact tcttctttga
      181 tctgccctct ttcagcgaaa tgttgcaggc atggacccgc agcggtgcgt tgcaggagca
      241 ggtggcaaac aaaatgcagg agtggtttga atctggcctg caacagtggg atatctcccg
      301 cgacgcacct tacttcggtt ttgaaattcc gaacgcgccg ggcaaatatt tctacgtctg
      361 gctggacgca ccgattggct acatgggttc tttcaagaat ctgtgcgaca agcgcggcga
      421 cagcgtaagc ttcgatgaat actggaagaa agactccacc gccgagctgt accacttcat
      481 cggtaaagat atcgtttact tccacagcct gttctggcct gccatgctgg aaggcagcaa
      541 cttccgcaag ccgaccaacc tgtttgttca tggctatgtg acggtgaacg gcgcgaagat
      601 gtccaagtct cgcggcacct ttattaaagc cagcacctgg ctgaatcatt ttgacgcaga
      661 cagcctgcgt tactactaca ctgcgaaact ctcttcgcgc atcgatgata tcgatctcaa
      721 cctggaagat ttcgttcagc gtgtgaatgc cgatatcgtt aacaaagtgg ttaacctggc
      781 ctcccgtaat gcgggcttta tcaacaagcg ttttgacggc gtgctggcaa gcgaactggc
      841 tgacccgcag ttgtacaaaa ccttcactga tgccgctgaa gtgattggtg aagcgtggga
      901 aagccgtgaa tttggtaaag ccgtgcgcga aatcatggcg ctggctgatc tggctaaccg
      961 ctatgtcgat gaacaggctc cgtgggtggt ggcgaaacag gaaagccgcg atgccgacct
     1021 gcaggcaatt tgctcaatgg gcatcaacct gttccgcgtg ctgatgactt acctgaagcc
     1081 ggtactgccg aaactgaccg agcgtgcaga agcattcctc aatacggaac tgacctggga
     1141 tggtatccag caaccgctgc tgggcca
//
LOCUS       DAJTYD010000313         1153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTYD010000313 DAJTYD010000000
VERSION     DAJTYD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.318"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            35..356
                     /locus_tag="OO585_003993"
                     /pseudo
     CDS             35..356
                     /locus_tag="OO585_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(390..596)
                     /locus_tag="OO585_003994"
     CDS             complement(390..596)
                     /locus_tag="OO585_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631391.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
BASE COUNT          330 a          247 c          249 g          327 t
ORIGIN      
        1 caatacataa ccattatctc ttgggattat ttacatgcgc aaaagtttaa tgctgttact
       61 tgcttcaaca ttgattattt ctggatgtgc aacaaaaaaa caactcgttc caactggtgg
      121 aagcaaatca gacggcacag ttaggatgag ttattcttac ggtatgtttg aaagtcctgt
      181 tgtagatcca caacaaggca tgaatgcagc caaagcgcgt tgtgccgctt aggggatatt
      241 caggagccga gccttttggt ggattcactt ctcaatgttc acaaccttct tcatctggtt
      301 gtatgcaaac acttgtgact atcgaatatc aatgcactgg cgatattaag aagtaataat
      361 taggggcttc cagccccttc tataaccatt catggaacag ggacaaccga aatcacttta
      421 aaccctgaaa cagccgttac tccattagat ggagaattaa atctcacagt atcttttctt
      481 actgcatcat gaatttcatc ttttgttggt tcatgctctg aatggaataa aacctcttcc
      541 attctaattt gatcaccgtc tctatgctca taagtaatct tgtagctaac agtcatatca
      601 acctcatgca ggggaattgt gagattatta tagacaatag aaaccataaa gccccgttgg
      661 tgatacagca acaagcggga gcgtagcgac ttcatcagat tgtcagaaag atcacacctt
      721 atcaggataa ccgtgtaccc gttgggagcg tggctgaaac gctgatagag cttgcatatg
      781 gtttgcaagg accaatatca ccataaacct gtttacgcag ccacaaggcc gcacatcggg
      841 cttaaatgcg tgtgttcagt ctgtcgatga cattacaatg ccaggatggg ctttatcttc
      901 acgttgtgct atcgcggtgt gagaggtgca actgccgcca acgttaggtt atactgtcat
      961 ctcaccgtgt aacttaacgt gacagttact aagggcggat cacaatggta gaactcctaa
     1021 gcaaattaaa taggtaactg tttaagttgc tgacagagaa gactttttta gtcttctcca
     1081 cctggggaga ttctggagcg ggcgaaggga atcgaaccct cgtatagagc ttgggaagct
     1141 ctcgttctac cat
//
LOCUS       DAJTYD010000314         1152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTYD010000314 DAJTYD010000000
VERSION     DAJTYD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.319"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..263)
                     /locus_tag="OO585_003995"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO585_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO585_003996"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO585_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6631392.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          337 a          282 c          176 g          357 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt ac
//
LOCUS       DAJTYD010000315         1146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTYD010000315 DAJTYD010000000
VERSION     DAJTYD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.320"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(135..314)
                     /locus_tag="OO585_003997"
     CDS             complement(135..314)
                     /locus_tag="OO585_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631393.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(314..937)
                     /locus_tag="OO585_003998"
     CDS             complement(314..937)
                     /locus_tag="OO585_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6631394.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
     gene            complement(888..>1146)
                     /locus_tag="OO585_003999"
     CDS             complement(888..>1146)
                     /locus_tag="OO585_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631395.1"
                     /translation="IAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIA
                     SPQFFITGCSEHSLPGSYCALSGGPDAHVSGAMPGLFIRTS"
BASE COUNT          247 a          345 c          284 g          270 t
ORIGIN      
        1 gtgatgcgga tgcggagagg gaagccggtg cacaggtgat gcgtgagact gaacagcaga
       61 tttaccgtca gctgactgac gaggtactgg ccctgcgatt gcctgaaaac ggctcacaac
      121 tgcaccattc ataatcacat cgcataaacc acagaccgga ctgactccgg aaaaacagag
      181 gcccgccccc gggcctcccc ggattcatcc gtttccctgt tcagcctgac agcacgcccc
      241 cgcggccgga tgacagactc cgcttcggta agcaaagcgg tcttctgtga ttccgccagt
      301 tgcggcttat tcattactca acgtcaaacg cccgaattga agccaaatca tccagaccgc
      361 tcagctcctc tttcatttca cgctgacggc gataaatctc atcgttgcga tcgacctgcg
      421 cctgcaccat tgctgccgcc agttcttcca gttccggcat cgacagtttc acctgctgat
      481 tatcggcatc gctccacgcc atatgtgttt gtgctgtgac agattttgcc agcatgacta
      541 ccggggacag gcggcccagt gagtcgggac cagcattcca gatacgaccg ttccattcaa
      601 acgtgaacgg cttcgcctcc tgttctgcgc gccatgcttc aatttcctga cgtctggcct
      661 ctctggccgc ttccagcatt tctggtgtca cagtgaatgg ggctatctca ccccatttgc
      721 cgctttgcag ttcctgccag atttgctgac ccgtcggagc gacatcatca gcggtggctg
      781 tgtaggggac tgcctggtcc ctgtcgtcaa aaaaaacgtc acagtctact gcgccacttt
      841 cggtataacg gggattaatg atttttttaa tttccacggt gcattcctca cgatgtgcga
      901 ataaaaagcc cgggcattgc gccagagaca tgtgcatccg gccccccgga cagggcgcaa
      961 tatgaccccg gtaatgaatg ctctgaacat cccgtaatga aaaattgtgg ggatgctata
     1021 tacgttccgg tgggagtact gggcactgaa atccccaccg gtcccagtcg tgagcctctg
     1081 tatgactgcc ccctgacaag tctgatgact ttatcaccgt cagcttctcc ctggtacgca
     1141 gcaata
//
LOCUS       DAJTYD010000316         1135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTYD010000316 DAJTYD010000000
VERSION     DAJTYD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.321"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            221..>1135
                     /locus_tag="OO585_004000"
     CDS             221..>1135
                     /locus_tag="OO585_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6631396.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIILADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTE"
BASE COUNT          267 a          277 c          339 g          252 t
ORIGIN      
        1 aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caataaaacg tgaagtaata
       61 tttcagctgg cgaataatac cccatagtag tcactctgcg caactgcgcg gtctttttcg
      121 aattgcgggc tgtcgtctct cttctgccat tgtcctgtaa cttccggact tcagcccgct
      181 cctcatttta ctcacaatat tatcccggcc gggaggattc atggctttta aacactatga
      241 tgttgtcagg gcggcgtcgc cgtcagatct tgcggaaaag ctgacacata aactgaaaga
      301 gggctggcag ccgtttggta gtccggtggc cataacccct tatactctga tgcaggcgat
      361 tgcagcagaa ggtgcagtaa tcagcgccac cagcgacccg gagtattact ttgttgtggt
      421 tctggcaggg cagtcaaacg gcatgtcgta tggtgaaggc cttccgctgc cggggacata
      481 tgaccgtccg gacccgcgta ttaagcaact ggcgcgtcgc agtacggtga caccgggcgg
      541 tgcagcatgc aaatataacg acatcattct ggcggaccat tgtctgcatg atgtgcagga
      601 catgagccgt cttaaccatc cgaaagcgga cctgtcaaag gggcagtacg gaaccgtggg
      661 gcaggggctg catatcgcca aaaaactgct gccgtttata ccggcgaatg cgggcattct
      721 gctggttccg tgctgtcgtg gtggttcagc gttcaccacc ggagctgatg gcacatacag
      781 tgacgcgagt ggtgcctcgg agaattcaac ccgctggggt gtggacaagc cgctgtataa
      841 ggaccttatc ggtcgaacaa aagcggcact ggagaagaac ccgaaaaatg tgctgtttgc
      901 cgtggtgtgg atgcaggggg aatttgattt tggcggtacg ccggcaaatc atgccgcaca
      961 gtttggtgcg ctggttgata aattccgtgc agacctggcg gatatggcag gccagtgcgt
     1021 cggtggctct gctggcggtg ttccctggat atgcggggac acgacgtatt tctggaagca
     1081 gaagaacgaa tccacgtacc agacggtgta cggcagctat aaaaataaaa cggaa
//
LOCUS       DAJTYD010000317         1131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTYD010000317 DAJTYD010000000
VERSION     DAJTYD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.322"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..617)
                     /locus_tag="OO585_004001"
     CDS             complement(<1..617)
                     /locus_tag="OO585_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631397.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQL"
     gene            complement(623..>1131)
                     /locus_tag="OO585_004002"
     CDS             complement(623..>1131)
                     /locus_tag="OO585_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6631398.1"
                     /translation="GFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKV
                     YAHFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGR
                     IMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGF
                     LSINKLSQ"
BASE COUNT          251 a          367 c          288 g          225 t
ORIGIN      
        1 ctcagttgtt caggaatatg gtgcagcagc tcgccgtcgc cgcagtaaat tgcggcgtga
       61 ttcggcactg atgaaccaaa acagcacagc agcacatcgc ccggctgtgc cgctgacaac
      121 ggcacctgat acagccccgt cgcctccaga ttatccagat agagattctg gccgttacgc
      181 caccagtcat cctcacgata aaagtccggc atctcaatcc ccgccagatg ataagcatcc
      241 cggaacagtg tgtaacagtc cgtcacaccg tgctcaaagc gccgcccggt gagatgcggc
      301 acacagcgga acttatgaat cgtcccccgg cagaccagcc accacggcaa atcactctgc
      361 acctgcagcc gccggtcggc ctcactcagc cagggcagac caccggggtg gctgtggacc
      421 agcgccacaa tctcaccctg catttctgcc tgcagccagt cttccggcga catacggaaa
      481 tacgcctccg gctcaccgga gatattcacg cagggaaaat atctttcccc ctccggcgtg
      541 cttaccacga agccgcacga ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct
      601 gattctgtct gtgtcatggg atttactgcg aaagtttgtt aatggaaagg aagccgccaa
      661 agttgccgac gttattgcgg aacttacaac cgctcaggca tttgctgcat ttatccttcg
      721 tgatatcgga cgttggctgg tcatattcat ccgcgacagc cggaccgcta taaccgcact
      781 cgtcaccgcg ataggtccag gtgcaggtgt tggccagcat gatacgtccc ggaaaaacag
      841 cgccatccgt ttccgtcggc gtggacagta caaaggaggc actcaccgcg ctcagttcgc
      901 tgcactgctc aatgcgccag cggctgatca cctcctgctc cggatcggcg taactgtttc
      961 cgttgacgaa gttcaccgca tccagaaaat gggcgtaaac cttacgccgg accaccgttc
     1021 cgccgaccag actctgcata tcttccgcca tcccggtgac cataccgtac aggttagaaa
     1081 ccgtcagcgt ggggcgcgta ctggtgcctt tgccattcag ttcaaaaccg c
//
LOCUS       DAJTYD010000318         1102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTYD010000318 DAJTYD010000000
VERSION     DAJTYD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.323"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            254..>1102
                     /locus_tag="OO585_004003"
     CDS             254..>1102
                     /locus_tag="OO585_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6631399.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          372 a          229 c          186 g          315 t
ORIGIN      
        1 tactgaacgt tatcgtgata tattcatcat gctttcttca gcattattcg agtacaggga
       61 gagacgcaat gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc
      121 agactccttc ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca
      181 aactgtttta aaattaataa gctatattct atttcactag aattcttatc gctgataaga
      241 aattttagtt ataatgatta aatcaaccaa tatacaggca atcggttctg gtattatgca
      301 tcaaataaac aatatatact cgttaactcc atttccttta cctatggaac tgactccatc
      361 ttgtaatgaa ttttatttaa aagcctggag tgaatgggaa aagaacggta ccccaggcga
      421 gcaacgcaat atcgccttca ataggctgaa aatatgttta caaaatcaag aggcagaatt
      481 aaatttatct gagttagatt taaaaacatt accagattta ccgcctcaga taacaacact
      541 ggaaataaga aaaaacctat taacacatct ccctgattta ccaccaatgc ttaaggtaat
      601 acatgctcaa tttaatcaac tggaaagctt acctgcctta cccgagacgt tagaagagct
      661 taatgcgggt gataacaaga taaaagaatt accatttctt cctgaaaatc taactcattt
      721 acgggttcat aataaccgat tgcatattct gccactattg ccaccggaac taaaattact
      781 ggtagtttct ggaaacagat tagacagcat tccccccttt ccagataagc ttgaagggct
      841 ggctatggct aataatttta tagaacaact accggaatta ccttttagta tgaacagggc
      901 tgtgctaatg aataataatc tgacaacact tccggaaagt gtcctgagat tagctcagaa
      961 tgccttcgta aatgttgcag gtaatccact gtctggccat accatgcgta cactacaaca
     1021 aataaccacc ggaccagatt attctggtcc tcgaatattt ttctctatgg gaaattctgc
     1081 cacaatttcc gctccagaac ac
//
LOCUS       DAJTYD010000319         1074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTYD010000319 DAJTYD010000000
VERSION     DAJTYD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.324"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..933
                     /locus_tag="OO585_004004"
     CDS             <1..933
                     /locus_tag="OO585_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6631400.1"
                     /translation="EIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVH
                     ADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAK
                     YQGVLQADAYAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELY
                     DIEAEIRGSPAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWN
                     ALNEFCRDGWVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNE
                     VEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT          289 a          239 c          331 g          215 t
ORIGIN      
        1 gaaatatacg cgcgacaggg cgtggagctg agccgtaata ccatggtgcg ctgggtatca
       61 gaaatggcag acaaactccg tcctctgtat atagcgctga atgactatgt tctggaggca
      121 ggaaaggtgc acgcagatga cactccggtg aaagtactgg ccccggggaa cggaaagacg
      181 aaaacgggtc gtctgtgggt atacgtcagg gatgatcgta atgcgggttc atccctgccg
      241 gcagccgtct ggttcgcgta ttcggcagat cgcaaaggag aacatccgca gctccacctg
      301 gcaaagtatc agggcgtact gcaggctgat gcctatgcag gttataacgt actgtacgaa
      361 acgggccggg tgaaggaagc cgggtgcctg gcccacgccc gccgaaaaat ccatgacgag
      421 gatgtgcgcc gtccgacaga aatgactcag gaagcgctca gacggatagc agagttatac
      481 gacatagaag cggagatacg tggcagtccg gcagaggaac ggcttgcagt cagaaaagcc
      541 agaagcgtcc agttgatgca gtcattgtac gactggatac agttgcagag gaaaacgctg
      601 tcgaaacatg cggagatggc gaaggcgttc gactatatcc tgaatcactg gaatgcgctg
      661 aacgagttct gtcgtgacgg ctgggtggaa atagacaaca acatcggtga aaacgcgtta
      721 cgatcggtgg cggttggaag aaaaaattat ctctttttcg gctcagacaa gggaggagaa
      781 agtgcggcga tcatctacag tctgctggtc acctgcaaac agaacgaagt ggagccggag
      841 gactggttgc gcgaagtgat cgagaagctc aatgactggc cgtcgaacca agtgcatgaa
      901 ctgctgccct ggaacttctc gtctgtaaaa taatccttac gctacgtact tctcggggcg
      961 cttacgattt atcgccgtca gcgggagctg aaggaagagc tgaatagtct gaaggatttg
     1021 aattcggtta ggaattttat cgtggaataa cagaagctgc ggcacgtcgt atgc
//
LOCUS       DAJTYD010000320         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTYD010000320 DAJTYD010000000
VERSION     DAJTYD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.325"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..440)
                     /locus_tag="OO585_004005"
                     /pseudo
     CDS             complement(1..440)
                     /locus_tag="OO585_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005314296.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            412..>1067
                     /gene="lafU"
                     /locus_tag="OO585_004006"
     CDS             412..>1067
                     /gene="lafU"
                     /locus_tag="OO585_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6631401.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHIDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     "
BASE COUNT          262 a          285 c          307 g          213 t
ORIGIN      
        1 tgggcgatat cgccgttgtc gctgacgcgg gtgaggataa tcgccgccgc ggtggagagt
       61 agcagggaag ggatttgcgc caccagcccg tcgccgatgg tcatcagcac atactgctgg
      121 aaggcggcgt cggcgctcag gttgtatttg aagatcccga tacagacgcc gccgatcagg
      181 ttgatcgcca gaatcatcat cccggcgata gcatccccgc gcacaaattt cgatgccccg
      241 tccatcgcgc cgtagaaatc ggcctcgctg gcaacatctt tacgtcgggt ttgtgcctgc
      301 gcctgattga tcaacccggc attaagatcg gcgtcaatcg ccatctgctt accgggcatc
      361 gcatccaggg taaagcgggc tgaaacctcg gaaatacgct cggccccttt ggtgacgacg
      421 ataaagttga ttgtcaacag cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg
      481 cacgggcagt cgatttttaa cggtggtggg ctgtcgccgt taaataaaat cagcccgtct
      541 catccgccaa aacctgccac cgtggcggtg ccggaagaaa cggagaaaaa agcgcgcgac
      601 gtcaatgaga aaacggcgct gctgaagaag aaatccgcca ccgagcttgg cgagctggca
      661 accagtatca ccaccattgc ccgtgatgcg catatggaag cgaatctgga gatggagatt
      721 gttccccagg gattacgcgt gctgattaaa gacgaccaga atcgcaatat gttcgaacgc
      781 ggcagcgcgc agattatgcc gttctttaaa acgctgctgg tggagctcgc gccagttttc
      841 gactcgctcg ataacaaaat tattattacc gggcatatcg atgcgatggc ttacaaaaac
      901 aatatctaca acaactggaa cctttcgggt gaccgcgcgc tttcggctcg tcgggtgctg
      961 gaagaggccg gaatgccgga agataaagtg atgcaggtaa gcgcaatggc ggaccagatg
     1021 ctgctggatt ccaaaaatcc gcaaagcgcg ggcaaccggc gcattga
//
LOCUS       DAJTYD010000321         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTYD010000321 DAJTYD010000000
VERSION     DAJTYD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.326"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            113..719
                     /locus_tag="OO585_004007"
                     /pseudo
     CDS             113..719
                     /locus_tag="OO585_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            822..1067
                     /locus_tag="OO585_004008"
                     /pseudo
     CDS             822..1067
                     /locus_tag="OO585_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
BASE COUNT          316 a          226 c          208 g          317 t
ORIGIN      
        1 ttgtatagat ctaacaaccg taataaaaca aatactcata actcattata aataatgata
       61 aaataacgtc ttttattgat aatttatata tgggaaaaat tccttttgtt atattgtttc
      121 tgatttcact ccctctgatt atttttacca accctgggtc actgcattac caccaacgcc
      181 ccgacacgtc agtcctgagc aattagaaaa aacagttcgt tatcttacac aaactgtgca
      241 tccacgtaat gccgacaata ttgataatct gaataggtcc gtagagtata taaaagagtt
      301 ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccgattggc tattacgact
      361 ctacgcctgg tagccaggat tacctttacc cgcaatgtcc tggctttatc ccgatcgggg
      421 ggactttatt gcctggtcgg cagaatacag gatatcaacg ccgttcgtca ggtaaaaacg
      481 gcattgttat catcccggga tttatctgtt tattctatga atgccccatg gtttattccc
      541 ggtattgatt tctctgacca cctgaattat tggcaacacg atattcccgc cgtaatgatt
      601 actgacaccg ctttttatcg taataaacaa taccacttgc ccggtgatac agcagacaga
      661 ttgaattatc agaaaatggc tcagatggtg ttataacctt gttacacaac agtaaataat
      721 aaaaatttct cgaggcgggt ccagaaacag cgacatgtac attttatttt ccctcgtctg
      781 ttttaagatg cgaaacataa aattcaatgg aggaaaaata aatgggaatg attggttatt
      841 ttgcagaaat tgactcagaa aagattaacc aatctgcttg agtctactga aaaacctttg
      901 atggataata tacatgacac gctttcagga ttacgccgac tggatattga taaacgctgg
      961 gattttttac actttgggct gacaggcacc cctgcttttg atcccgcgaa gaatgatccg
     1021 ctgagtcgtg ccgtgcttgg tgaacacagc ctcgaagatg gcataga
//
LOCUS       DAJTYD010000322         1040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTYD010000322 DAJTYD010000000
VERSION     DAJTYD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.327"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..86
                     /gene="ortT"
                     /locus_tag="OO585_004009"
     CDS             <1..86
                     /gene="ortT"
                     /locus_tag="OO585_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6631402.1"
                     /translation="RIRFLSAFLVGATWPMSFPVALLFSLF"
     gene            172..405
                     /locus_tag="OO585_004010"
     CDS             172..405
                     /locus_tag="OO585_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6631403.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(406..855)
                     /locus_tag="OO585_004011"
     CDS             complement(406..855)
                     /locus_tag="OO585_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6631404.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(852..>1040)
                     /gene="mddA"
                     /locus_tag="OO585_004012"
     CDS             complement(852..>1040)
                     /gene="mddA"
                     /locus_tag="OO585_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6631405.1"
                     /translation="SGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLD
                     LTFMQLQLDERTEPDAIG"
BASE COUNT          279 a          277 c          264 g          220 t
ORIGIN      
        1 aacgcatccg tttcttaagc gcctttctgg tgggggcaac atggccgatg agtttcccgg
       61 tggcgctgtt gttttcactg ttttaaatct tactcactgg cttgacggca ccacgcagac
      121 ttatatcatt tggatgaatc gataaatttc acaagtggtt aaggagaaag tatgtcgcat
      181 ctggatgaag tcatcgcgcg cgtggatgcc gcgattgaag agagcgtcat tgcccatatg
      241 aacgaattat taatcgccct gagcgatgac gcggagttaa gtcgggaaga tcgctacacc
      301 cagcagcagc gtttgcgcac cgctattgct catcacggtc gcaagcataa agaagatatg
      361 gaagcgcgcc acgaacagtt aaccaaaggc ggcaccatcc tctgattaaa atgagcgtct
      421 ggctaccaac caggcgccaa ccacaagtaa aattgcgcca caaattgggc cgaacaacgc
      481 ccgtagtggt acgccatgac tgcggaagat atccagcatc agaccgccaa ttagctggct
      541 ggcgaccagt actgcaatag ttgtcgccgc tcccacattc tgatatccgc tgatactggc
      601 gaagacaaaa aacgatccca gcaagccggg tataagtgtc caccagcgca cgctggacac
      661 cagttcgcca aaccccgcca tgccttgttt aaaccataaa atagaaacaa agagcacaat
      721 ccccaccagt gagttcagca acatggcgat gagaatggta gaggaggtct gggtgatgcg
      781 caccatcagc gtgttctgga ctaccagacc aattccggcg gcaatcaaaa aagcaagggt
      841 gagcgactga ttcatccaat cgcgtccggt tcagtgcgtt cgtcgagttg caactgcata
      901 aatgtcagat ccagccagcg cccaaattta gtgcccacct gcggcatttg tgcggtgacg
      961 acaaatccca gcgaatggtg cagatgcagc gaggcctgat tttgcgattc gatcccggcg
     1021 accatgacat gcttcccgct
//
LOCUS       DAJTYD010000323         1040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTYD010000323 DAJTYD010000000
VERSION     DAJTYD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.328"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..830
                     /locus_tag="OO585_004013"
     CDS             <1..830
                     /locus_tag="OO585_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCR6631406.1"
                     /translation="SMKVYQLPEATRGARGRPIVNLLPLEQDERITAILPVTEFEEGV
                     KVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDELIGVDLTSGEDEVMLFSAEGK
                     VVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRGDGAILTATQNGYGKRTAVAE
                     YPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMITDAGTLVRTRVSEISIVGRN
                     TQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAAEGDDEIAPEVDVDDEPEEE"
BASE COUNT          256 a          242 c          310 g          232 t
ORIGIN      
        1 attcgatgaa agtttatcag ttgccggaag ccactcgtgg cgcgcgcggt cgtccgatcg
       61 tcaacctgct gccgctggag caggacgaac gtatcactgc gatcctgcca gtgaccgagt
      121 ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg taccgtgaag aaaactgtcc
      181 tcaccgagtt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg gttgacggcg
      241 atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg ttctccgctg
      301 aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc aacaccaccg
      361 gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc gtgcctcgtg
      421 gcgatggcgc aatcctcacc gcaacacaaa acggttacgg taaacgtacc gcagtggcgg
      481 aatacccaac caagtcgcgt gcgacgaaag gggttatctc catcaaggtt accgaacgta
      541 acggtttagt tgttggcgcg gtacaggtag atgactgcga ccagatcatg atgatcaccg
      601 atgccggtac gctggtacgt actcgcgttt cagaaatcag catcgtgggc cgtaacaccc
      661 agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg caacgtgttg
      721 ctgaaccggt tgacgaggaa gatctcgata ccatcgacgg cagtgccgcg gaaggggacg
      781 atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa ttttacttct
      841 tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct gcaaagtctg
      901 ggcttttgtc gtattagggc acggtaaagt ttggctgtgc ccgtaaaaaa tggctggcta
      961 tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg gttctatggt
     1021 cttgtaggtt ttggcgcaat
//
LOCUS       DAJTYD010000324         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTYD010000324 DAJTYD010000000
VERSION     DAJTYD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.329"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..695)
                     /locus_tag="OO585_004014"
     CDS             complement(<1..695)
                     /locus_tag="OO585_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6631407.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          302 a          171 c          220 g          335 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac gttaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagttct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggaa ataataagct aaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa tgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagc
//
LOCUS       DAJTYD010000325         1011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTYD010000325 DAJTYD010000000
VERSION     DAJTYD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.330"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1011
                     /locus_tag="OO585_004015"
     CDS             <1..>1011
                     /locus_tag="OO585_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR6631408.1"
                     /translation="RAKEVVLKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPE
                     FYHYPGRLQGYINAYLTETDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEK
                     PQPSDAMAGEQATTETMEPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAAN
                     ISQPDADKLLAVSRGEFVEGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSP
                     NALQNEPETKQSEPVAQQEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVE
                     VQENDPEEMEGAEHPHKENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHL
                     MI"
BASE COUNT          330 a          253 c          255 g          173 t
ORIGIN      
        1 tcgcgcaaaa gaagtagtcc tgaaaaaaga aagtccgttc aaagaatggt ccgcagcact
       61 tcgtgcaccc ccgggtattc tggactattc ccgcgccgct atttttgcac ttatccgaag
      121 cgcacaccct gaattttatc actacccggg acgccttcag gggtatatca acgcctattt
      181 gacggaaact gatcacgaga accccagcaa ggaaactctc acagctaccc ggcatacgcc
      241 ggaaaaagat atcctggaag aaattaaccg cgaggtggtt actgagcgtg aaacagaaga
      301 agaaaaacca caaccatctg acgcaatggc aggtgaacag gcaacaactg aaacaatgga
      361 accggataca actgaacatt gccagaacgc gcagtcgctg gatgctcagt cgcaggtgag
      421 ttccgctaac caagtaaaag tcaccgctga cgaagtaaac aaaattatgc aggcagccaa
      481 tatcagccag cctgacgccg ataagttact tgctgtatcg cgtggtgaat ttgttgaggg
      541 gattagcgac cctaatgatc cgaaatgggt caaggggatc cagactcgcg attctgtgaa
      601 ccagaaccag catgaatcgg aacggaacga ccaaaaagcg gaacaaaaca gcccaaatgc
      661 gttacaaaac gagccagaaa cgaaacaatc cgaaccagta gcgcaacagg aaccggaaaa
      721 agtctgcacc gcctgcggtc agagcggtgg cggcaactac cctgattgtg gcgcggtgat
      781 ggacgacgca acataccaga aaacattcga tgaagagaat caggttgaag ttcaggaaaa
      841 tgatccggag gaaatggaag gcgctgaaca tccacacaag gagaatgctg gcagcgctca
      901 ggaccacgcc agcgatagta aaactggcga gacggcagat cccttaatta cggtgaatgg
      961 tcatcacgaa atcacatcca ccagcaggac gtgtgaccat ctaatgatcg a
//
LOCUS       DAJTYD010000326         1010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTYD010000326 DAJTYD010000000
VERSION     DAJTYD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.331"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO585_004016"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO585_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6631409.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO585_004017"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO585_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6631410.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1010)
                     /locus_tag="OO585_004018"
     CDS             complement(941..>1010)
                     /locus_tag="OO585_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR6631411.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          301 a          206 c          231 g          272 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc
//
LOCUS       DAJTYD010000327         1003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTYD010000327 DAJTYD010000000
VERSION     DAJTYD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.332"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..976
                     /gene="ygeW"
                     /locus_tag="OO585_004019"
     CDS             <1..976
                     /gene="ygeW"
                     /locus_tag="OO585_004019"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6631412.1"
                     /translation="RTRFSYASALNLLGLAQQDLDEGKSQIAHGETVRETANMISFCA
                     DAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADLA
                     WLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYDL
                     IPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRANDH
                     EGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTEG
                     VFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT          268 a          272 c          256 g          207 t
ORIGIN      
        1 ccgtacccgc ttctcttatg cttccgcgct taacctgctc ggccttgcac aacaagatct
       61 cgacgaaggc aaatcacaaa tcgctcacgg cgaaaccgtg cgtgaaaccg ccaatatgat
      121 ctccttctgc gccgacgcta ttggtattcg cgacgatatg tatctgggcg caggcaacgc
      181 ctatatgcgt gaagttggcg ctgcacttga tgacggttac aagcagggtg tactgccaca
      241 gcgtccggct ttagtgaacc tgcaatgcga tattgaccac ccgactcagt caatggctga
      301 cctcgcgtgg ttacgtgaac actttggttc actggaaaac ctgaaaggta aaaaaatcgc
      361 catgacctgg gcctactctc caagctatgg caaaccgctc tctgtaccac aaggcatcat
      421 cggtctgatg actcgcttcg gtatggatgt caccctggcc catccggaag gctacgacct
      481 gatcccggat gtggttgaag tggcgaaaaa caatgctaaa gcctccggtg gtagcttccg
      541 tcaggtcacc agcatggaag aagccttcaa agacgcagac atcgtttatc cgaagtcatg
      601 ggcaccttac aaagtgatgg aagagcgtac tgaattgctg cgtgcgaacg accacgaagg
      661 cttaaaagca ctggaaaaac agtgtctggc acagaacgcg caacacaaag actggcattg
      721 tactgaagag atgatggaac tgacccgtga tggcgaagcc ctgtacatgc actgcctgcc
      781 agctgatatc agcggcgtat cctgcaaaga aggtgaagtg actgaaggcg tattcgaaaa
      841 ataccgtatc gctacctaca aagaagccag ctggaagcct tatatcatcg ccgcgatgat
      901 cctgtcccgt aaatacgcca aacctggtgc actgctcgag caactgctga aagaagcgca
      961 agaacgcgtg aaataacccc tcgggccgat cagctggtcg gcc
//
LOCUS       DAJTYD010000328         1002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTYD010000328 DAJTYD010000000
VERSION     DAJTYD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.333"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..212)
                     /locus_tag="OO585_004020"
     CDS             complement(<1..212)
                     /locus_tag="OO585_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR6631413.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNR"
     gene            637..>1002
                     /gene="ospC2"
                     /locus_tag="OO585_004021"
     CDS             637..>1002
                     /gene="ospC2"
                     /locus_tag="OO585_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6631414.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHP"
BASE COUNT          328 a          190 c          187 g          297 t
ORIGIN      
        1 catcggttaa agcggttata aacagttttc catgccccat atcgttcggg taaatcgcgc
       61 catggtgcac cggaacataa cacccagaac atgccattga tgattttacg gtgctcagcc
      121 catgggcgtc cggcccgtgg tgtggcgggt tcaggaggta acaggggctt gatgatggtc
      181 catgcctcat cgggaaggtc gtagcgagcc atagttcaat atgttgtgta aacagacagt
      241 tactataact cagatgatta agggacacag cctactattc cgcgcagcta tttagctctc
      301 tttttgttga gcgcccatga ttctttcatt tattgttact aattttttat gttatcatca
      361 tgttatgagc atgccctgtt ctgcgtctct attttatcta tcatattctg agcattaact
      421 acataacaag gcgcaagata tatatccaaa aacaaataat actgttgcaa taattccttt
      481 cagtaaagga agtgtgatta aactcagggg atttcagagt gaaaatcgta caacatatta
      541 aaatatgttc gttataatac atacaagaaa atattaagtt agataatatt attctaaata
      601 actgcagata aaaacgcaca taatggggga tgttttatga aaatacctga agcagtaaat
      661 catattaatg ttcagaacaa tattgatctt gttgatggaa aaataaatcc gaacaaagat
      721 acaaaagcat tacaaaaaaa catatcatgc gtaacaaact catcctcttc tggcataagt
      781 gaaaaacatt tagatcactg tgctgatact gtgaaaagct ttttaaggaa gtctatagca
      841 gcgcaaagtt atagtaaaat gttctctcaa gggaccagtt tcaaatcatt aaatctttct
      901 attgaagcgc catctggagc acggtcatca tttcggtcac ttgagcacct cgataaggtg
      961 tccagacact atctttctga aataatacaa aaaactcatc ct
//
LOCUS       DAJTYD010000329         1001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTYD010000329 DAJTYD010000000
VERSION     DAJTYD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.334"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>1001
                     /locus_tag="OO585_004022"
     rRNA            <1..>1001
                     /locus_tag="OO585_004022"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          252 a          233 c          320 g          196 t
ORIGIN      
        1 ggtaacggct caccaaggcg acgatcccta gctggtctga gaggatgacc agccacactg
       61 gaactgagac acggtccaga ctcctacggg aggcagcagt ggggaatatt gcacaatggg
      121 cgcaagcctg atgcagccat gccgcgtgta tgaagaaggc cttcgggttg taaagtactt
      181 tcagcgggga ggaagggagt aaagttaata cctttgctca ttgacgttac ccgcagaaga
      241 agcaccggct aactccgtgc cagcagccgc ggtaatacgg agggtgcaag cgttaatcgg
      301 aattactggg cgtaaagcgc acgcaggcgg tttgttaagt cagatgtgaa atccccgggc
      361 tcaacctggg aactgcatct gatactggca agcttgagtc tcgtagaggg gggtagaatt
      421 ccaggtgtag cggtgaaatg cgtagagatc tggaggaata ccggtggcga aggcggcccc
      481 ctggacgaag actgacgctc aggtgcgaaa gcgtggggag caaacaggat tagataccct
      541 ggtagtccac gccgtaaacg atgtcgactt ggaggttgtg cccttgaggc gtggcttccg
      601 gagctaacgc gttaagtcga ccgcctgggg agtacggccg caaggttaaa actcaaatga
      661 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgatgca acgcgaagaa
      721 ccttacctgg tcttgacatc cacggaagtt ttcagagatg agaatgtgcc ttcgggaacc
      781 gtgagacagg tgctgcatgg ctgtcgtcag ctcgtgttgt gaaatgttgg gttaagtccc
      841 gcaacgagcg caacccttat cctttgttgc cagcggtccg gccgggaact caaaggagac
      901 tgccagtgat aaactggagg aaggtgggga cgacgtcaag tcatcatggc ccttacgacc
      961 agggctacac acgtgctaca atggcgcata caaagagaag c
//
LOCUS       DAJTYD010000330          979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTYD010000330 DAJTYD010000000
VERSION     DAJTYD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.335"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..237
                     /locus_tag="OO585_004023"
     CDS             <1..237
                     /locus_tag="OO585_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631415.1"
                     /translation="LFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLN
                     KMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(206..>979)
                     /locus_tag="OO585_004024"
     CDS             complement(206..>979)
                     /locus_tag="OO585_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR6631416.1"
                     /translation="VMLVFTLPGFDRVFKVIKDKFAPQKEMSAAHVRACYQLVKEHDR
                     VGRMADTQEFENFVLEKRHISPALMELLLQEAAEKITDLGEQIVIRHLYIERRMVPLN
                     IWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICY
                     MTEVNFRDIPPPRYPEDELASELWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADL
                     FRADYWRALQNRIREGHVEDVYAYRRRQRFSVRYGEMLF"
BASE COUNT          251 a          257 c          227 g          244 t
ORIGIN      
        1 ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc gccacaaaaa
       61 tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga attaatcctt
      121 aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg atacattgcg
      181 gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt acgctaaatc
      241 tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg cggttttgta
      301 gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac agcggaccaa
      361 tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg cccggcgaga
      421 cgctgtacca cagttcgctg gcaagttcgt cttccggata gcgcggcggc gggatgtcgc
      481 ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc acacgcccgt
      541 gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta gcagcggcaa
      601 gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga ccttccactt
      661 gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg cgaattacaa
      721 tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat tccattaatg
      781 ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg tccgccattc
      841 ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga gcggcagaca
      901 tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca aagcccggca
      961 gcgtaaacac caacatcac
//
LOCUS       DAJTYD010000331          968 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTYD010000331 DAJTYD010000000
VERSION     DAJTYD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.336"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..812
                     /gene="mppA"
                     /locus_tag="OO585_004025"
     CDS             <1..812
                     /gene="mppA"
                     /locus_tag="OO585_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6631417.1"
                     /translation="PKNMYQKLLKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLA
                     LSMTIDRRLMTEKVLGTGEKPAWHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSA
                     AGYGPQKPLKLTLLYNTSENHQKIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGN
                     FDVIRASWVGDYNEPSTFLTLLTSTHSGNISRFNNPAYDKVLAQASTENTVKARNADY
                     NAAEKILMKQAPIAPIYQYTNGRLIKPWLKGYPINNPEDVAYSRIMYIVKH"
BASE COUNT          287 a          214 c          236 g          231 t
ORIGIN      
        1 tcccgaaaaa tatgtatcag aagctgttga aggatattcc ggggcaggtt tatacgccgc
       61 cgcagctcgg gacctattat tatgcgttta acacgcaaaa agggccgacg gcagatcagc
      121 gcgttcgcct ggcattaagt atgacgatag atcgccgcct gatgaccgaa aaagtgttgg
      181 gaacgggcga aaagccagcg tggcatttta caccagatgt taccgccgga tttacgccgg
      241 aaccttcgcc gtttgaacaa atgagtcagg aagaactgaa tgcgcaggca aaaactttgt
      301 tgagcgcagc tggttatggt ccgcaaaaac cgctgaagct gacgcttttg tataacactt
      361 cagaaaacca tcaaaaaatt gcgattgctg tagcatcgat gtggaaaaag aaccttggcg
      421 tagatgttaa attgcaaaat caggaatgga aaacctatat cgatagccgt aacaccggca
      481 attttgatgt tatccgcgcc tcatgggtgg gggattataa tgaaccctcc actttcctga
      541 cattattaac gtcaacgcat tcaggaaata tttcacgctt taacaatccg gcatacgaca
      601 aagttctggc ccaggcatcg acggaaaata ccgttaaagc gcgtaatgcc gattacaacg
      661 cggcagaaaa aatcctcatg aagcaagcac cgattgcacc aatttatcaa tataccaatg
      721 gacgattaat caagccgtgg ctgaaaggtt atcctattaa taacccggaa gatgtggcgt
      781 acagccggat tatgtatatt gtgaagcatt gatgtgatgg gaactggcgt tacccttgtg
      841 gataacgcca gtgatgtctg atttagcgcc cctgttccgg cggtgtaatg ttatccatat
      901 acagcgtctg gctggggaag gcaaagtccg cgccgtgtga ctgcacaata tcgataatct
      961 tcaaataa
//
LOCUS       DAJTYD010000332          966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTYD010000332 DAJTYD010000000
VERSION     DAJTYD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.337"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..483)
                     /locus_tag="OO585_004026"
     CDS             complement(1..483)
                     /locus_tag="OO585_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6631418.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OO585_004027"
     CDS             complement(474..776)
                     /locus_tag="OO585_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6631419.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          294 a          238 c          194 g          240 t
ORIGIN      
        1 tcagacctta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcc
//
LOCUS       DAJTYD010000333          946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTYD010000333 DAJTYD010000000
VERSION     DAJTYD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.338"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..567)
                     /gene="mscS"
                     /locus_tag="OO585_004028"
     CDS             complement(<1..567)
                     /gene="mscS"
                     /locus_tag="OO585_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6631420.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFI"
BASE COUNT          280 a          238 c          222 g          206 t
ORIGIN      
        1 aataaattcg ttacggcgaa ctggctcgcg ggagaagtta ataatatttc cggcaataat
       61 tttaccgttc ggaataacga taattttacc gtctgcagta cgcatggtgg tggagaaaat
      121 ctgcacactc agcacagtac cggctacgcc gccgaggtca acatattctc cggcacggaa
      181 cggtcggaac atgacaagta acacgccagc ggccaggtta gaaagtgacc cctgcaaagc
      241 cagaccaaca gctaagcctg cggcaccgag tacagcaatg actgacgctg tttgtacacc
      301 cacgcgtccc agggcagcga ttagcgtaaa ggcgataata ccgtaacgga ctaatgcaga
      361 aagaaaatca gcaacagtgg catcgatttt acgggagatc atcaggcgat tcaccgcgtt
      421 ggaaatcatc cgcgcgataa tcaaaccaac gatgatgatc gcgagtgccg ccacgatgtt
      481 tactgcataa cttagcagca gcgcctggtt agctaccagc cagcttcccg cgccgtttat
      541 gctatcgaca acattcaaat cttccattca atattccttt tcaactgact caaaatggag
      601 aaatacactc cgccgcctta tgacgggcag tctgacaagg gtaaacaata aagcataatt
      661 ttgccaaata ggtcacagat ttctcatctg aatcaccttg cttttggcga aaaaatcccc
      721 gcagagcggg gctcagacag atatcaatgt gcgctttgtc atgccggatg cggcgtgaac
      781 gccttatccg gcctacaaaa acctgcaaat tcaatatatt gcaggatacg tgtaggcctg
      841 ataagcgtag cgcatcaggc aattttgctt ttggcgaaaa aaagacccgc agagcgggcc
      901 ttgagataag cagaaaggaa tatcttacag aacgtcgatc gcgttc
//
LOCUS       DAJTYD010000334          940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTYD010000334 DAJTYD010000000
VERSION     DAJTYD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.339"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>940
                     /locus_tag="OO585_004029"
     CDS             <1..>940
                     /locus_tag="OO585_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631421.1"
                     /translation="YTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGG
                     MLSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSM
                     REDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVG
                     NLIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQ
                     LVTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYD
                     AAEKAQNSATISIIIALFTIVPAICYLLSAIIAKRY"
BASE COUNT          193 a          228 c          244 g          275 t
ORIGIN      
        1 cttacaccct acttacctta ctttataccg tcgtcaatat cccttactgc gcattgggtg
       61 gtgtaatcac caatgacccg actcagcgta tctcgctgca atcctggcgt tttgtgctgg
      121 cgaccgcggg aggcatgctt tctactgttc tgatgatgcc actggttaat ttaattggcg
      181 gtgataataa accactcggt ttccagggcg gtatcgcggt cctttccgtg gtggcattca
      241 tgatgctggc attttgtttc ttcaccacta aagaacgcgt tgaagcacca cctacaacaa
      301 cgtctatgcg ggaagattta cgtgatatct ggcaaaacga ccagtggcgg attgtcggtt
      361 tactaaccat tttcaatatc ctggcggtgt gcgtactcgg tggggcgatg atgtattacg
      421 tcacatggat tttgggcacg ccggaagtgt ttgtcgcttt tctcaccact tattgcgtgg
      481 gtaacctgat tggttccgca ctggcaaaac ctctgaccga ctggaaatgt aaagtcacta
      541 tcttctggtg gacgaacgcc ctgctggcag tgattagcct cgcgatgttc tttgttccca
      601 tgcaggccag catcactatg tttgtcttca tcttcgtgat tggtgtgttg catcaactgg
      661 tgacacctat ccagtgggta atgatgtccg ataccgtcga ctacggcgag tggtgcaatg
      721 gtaaacgcct gaccgggatc agttttgctg gcacgctgtt tgtgctcaaa ctggggttgg
      781 ccttcggcgg cgctcttatc ggctggatgc tggcttatgg cggatatgat gcggcagaaa
      841 aagcgcagaa cagcgccacg attagcatca ttattgcgct attcacgatt gttccggcga
      901 tctgttattt gctgagcgcg attatcgcta aacgctacta
//
LOCUS       DAJTYD010000335          933 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTYD010000335 DAJTYD010000000
VERSION     DAJTYD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.340"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..873)
                     /locus_tag="OO585_004030"
     CDS             complement(<1..873)
                     /locus_tag="OO585_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6631422.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQG"
BASE COUNT          235 a          253 c          234 g          211 t
ORIGIN      
        1 accctgtttt gccgagtcag gcacatccag tagttgaata aatgcctcaa gcggacgcgt
       61 tttcgcacca cgacgccaga ccagccaggt tgttaaccaa cgccattgct cagctaacgg
      121 ccacgcttca acctggtgat gccccggcat actttccagc atagagcgtg gaataagcgc
      181 aatgcctgct cctgcgacca cacaggccaa catcccgtga tacgactcca tctcgtgaat
      241 agttcccgga gcggcaccgt cagcatgaaa ccagctctcg aagtggcgac gatacgaaca
      301 attggcgcgg aaggcataaa tgttactgcc gttaacctga ctggcgcgga ttactggcgc
      361 atgaccttgt ggcgtgacga tcatcagctc ctcgcggtat accggtatcc cgtcaatggc
      421 agtatggtta atgggcccat caataaatgc cgcattcagt tttccctcca gcactccttc
      481 cagcatagtg ccggaagggc cggtggaaag tgaaaactga attttgggat aacgatggtt
      541 gaattccgcc agcgtggccg gaatgcgcac tgctgcggtg ctttccagcg aaccaagaga
      601 aaataaacct tgcggctcat cgccagcgac aacgctccgc gcttcatcca ctaacgcgag
      661 aatttgttgg ctatagcgta aaaagttatg cccggcagga gagagacgta aacgctgatt
      721 ctcgcgaata aacagatcta cccccagttc tgtttccagc tggcgtagac ggatagtgag
      781 attcgacggc acgcgaagca ctattgctgc tgcctgggtt atgctgccag cttcggcaac
      841 cgcgttgaac atctccagtt gggtcagatc cataatattc tcgattcgtg aataagtggc
      901 ttaatattat tcattttaaa gcaagagtaa atc
//
LOCUS       DAJTYD010000336          929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTYD010000336 DAJTYD010000000
VERSION     DAJTYD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.341"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>929
                     /locus_tag="OO585_004031"
     CDS             <1..>929
                     /locus_tag="OO585_004031"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310436.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6631423.1"
                     /translation="ASFAGQQETFLNVQGFDAVLVAVKHVFASLFNGRAISYRVHQGY
                     DHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPD
                     EFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNE
                     EVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQ
                     GKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVT
                     NRGGRTCHAAIIARELGIPAVVGCGDATERMKD"
BASE COUNT          205 a          248 c          277 g          199 t
ORIGIN      
        1 cgcttccttt gccggtcagc aggaaacctt cctcaacgtt caggggtttg acgccgttct
       61 cgtggcagtg aaacatgtat ttgcttctct gtttaacggt cgcgccatct cttatcgtgt
      121 gcaccagggt tacgatcacc gtggcgtggc gctctccgcc ggtgttcagc ggatggtgcg
      181 ctccgacctc gcatcttctg gcgtgatgtt ctccattgat accgaatccg gctttgacca
      241 ggtggtgttt atcacttccg catggggcct tggtgagatg gtcgtgcagg gggcggttaa
      301 cccggatgag ttttacgtgc ataaaccgac actggcggcg aatcgcccgg ctatcgtgcg
      361 ccgcaccatg gggtcgaaaa aaatccgcat ggtttacgcg ccgacccagg agcacggcaa
      421 gcaggttaaa atcgaagacg taccgcagga acagcgtgac atcttctcgc tgaccaacga
      481 agaagtgcag gaactggcaa aacaggccgt acaaattgag aaacactacg gtcgcccgat
      541 ggatattgag tgggcgaaag atggccacac cggcaaactg ttcattgtgc aggcgcgtcc
      601 ggaaaccgtg cgctcacgcg gtcaggtcat ggagcgttat acgctgcatt cacagggtaa
      661 gattatcgcc gaaggccgtg ctatcggtca tcgcatcggt gcgggtccgg tgaaagtcat
      721 ccatgacatc agcgaaatga accgcatcga acctggtgac gtgctggtca ctgacatgac
      781 cgacccggac tgggaaccga tcatgaagaa agcatctgcc atagtcacca accgtggcgg
      841 tcgtacctgt cacgcggcga tcatcgctcg tgaactgggt attccggcgg tcgtgggctg
      901 tggtgatgca acagaacgga tgaaagacg
//
LOCUS       DAJTYD010000337          927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTYD010000337 DAJTYD010000000
VERSION     DAJTYD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.342"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            132..927
                     /locus_tag="OO585_004032"
                     /pseudo
     CDS             132..927
                     /locus_tag="OO585_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          301 a          136 c          162 g          328 t
ORIGIN      
        1 cttcacttct tcttcactaa gaatgtccat tatctcaatg ccttatcaga gattcttttc
       61 ctttcgccgg tagtgtctgg acgctcaggc tacttttcca ggttatttta tttctgtatt
      121 acagaggttt tatgataggt catatcacaa attctggtgg caataactct ttgatgaaac
      181 atgatgtggt tcaaggaaat aatatagtag atcttgattt actacgtaat ttcaatgggg
      241 tgacaggttt aaacagagat aactttattt atatcagtaa tatattttta aatataaaac
      301 aacggaacga aaaaatcatg caataaatat gttacgtgaa gtctcaatta gtaatgatac
      361 tataagtgta aaattctaca gaaatgaaaa aatagaatgc gcttgtaatt ttctgatgga
      421 taaagattcg caggggtata tcgacctgtc tgattttgat gtaacaaatt gtcattttaa
      481 aggtgacgtt atttctaagg tgtctttttt atcatcaaat ctacaacatg taactttcga
      541 atgtaaagaa attgagaatt gcaattttac taaggcaaca gttgataatg tcatatttaa
      601 atgtcgacgt ttacacaatg tgatttttct caaaacgagt ggcgaatgtg ttgattttaa
      661 ccaagatatt cttgatacag ttgacttctc gcagagtcaa cttggtcata gtaattttcg
      721 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
      781 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      841 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      901 ggaattaact attgattatt cagatat
//
LOCUS       DAJTYD010000338          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTYD010000338 DAJTYD010000000
VERSION     DAJTYD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.343"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="OO585_004033"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="OO585_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6631424.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          230 c          264 g          208 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAJTYD010000339          908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTYD010000339 DAJTYD010000000
VERSION     DAJTYD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.344"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..908
                     /locus_tag="OO585_004034"
                     /pseudo
     CDS             1..908
                     /locus_tag="OO585_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          257 a          221 c          243 g          187 t
ORIGIN      
        1 gattatccct tcctgaaggc actttaggac aatgggttac cgccgccaga aaagggctcg
       61 gtactcctgg ttcccgcacg gtagctgaac tggaatctga aattctgcaa ctgcgtaagg
      121 cgttaaatga agctcgcctt gagcgagata tattaaaaaa agcaacagcg tattttgcac
      181 aggagtcgct gaaaaatacg cgttaatcga acaatggcga caacaatttc ccattgaagc
      241 gatgtgtcag gtatttggtg tatccaggag cggttattac aactgggtac agcatgaacc
      301 ctcagacaga aaacaaagtg atgagcggct aaaactggag attaaggtgg cacatatccg
      361 cactcgcgaa acatatggaa cccggcggct ccagacggag ctggcagaga atggcatcat
      421 cgttggtcgt gaccgactgg cacgtcttcg taaggagcta aggctacgct gtaagcagaa
      481 acgcaagttc agagcgacta cgaacccgaa ccacaatctg ccagttgcgc caaatctgct
      541 gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg gtggcggacc tgacgtatgt
      601 tgccacacag gagggatggt tgtacctcgc tggcatcaaa gatgtttata cgtgcgaaat
      661 tgtcggctac gccatgggag agcgcatgac aaaagagctg acaggtaaag ccctgtttat
      721 ggcgctcagg agccagcgcc cacctgccgg gctaatccac cactctgatc gaggttcaca
      781 gtactgcgca tacgattacc gggtcataca ggagcagtct ggtctgaaaa catcaatgtc
      841 gcgtaaaggt cactgttacg acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa
      901 tgagagcc
//
LOCUS       DAJTYD010000340          900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTYD010000340 DAJTYD010000000
VERSION     DAJTYD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.345"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>900
                     /locus_tag="OO585_004035"
     CDS             <1..>900
                     /locus_tag="OO585_004035"
                     /inference="COORDINATES: protein motif:HMM:NF019996.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6631425.1"
                     /translation="GYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLR
                     PEVMKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAE
                     DRKATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIA
                     AAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDT
                     KSDFLAWVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTF
                     YGINGPIYHPRYCYWPISRLTDWV"
BASE COUNT          227 a          236 c          272 g          165 t
ORIGIN      
        1 ggggtattac gaggttgcgc ttcactgtaa cggctggcag ccaacccgtg tcggggatat
       61 tgatgtggca ccgactgatg caccggggac actgaacgcg tttctgaatg caccaaagga
      121 tggtgattta cgtccggagg tgatgaagcg ctttgaggaa atggtggtgc aggcgcagca
      181 gagcgccggg gccgcagccg gaaacgcaca gcagacggcg caggatgtgg cggcagccgc
      241 aacggcccgt gatgatgcac aacgttttgc ggagaatgcc agacaggatg caaccgtcac
      301 agctgaggac agaaaggcca ctgcggaaga tgtgacaagc acaggagcaa atgcagccgc
      361 agccggacag agcgcacagg atgccgcagg ttatgcccgc gcagcagaac aggccaaaaa
      421 tgacattgat gctgcgctga ccggcactct gaaaatggct aaccatctgt cagaaatcgc
      481 agcagcaggc gaaaaggcac aacagaagtc ccgggataat ctggggctga aaagtgcggc
      541 cacgatggaa gcacagagcg atatttacga ccggacaaaa ggccgtctgg cgatacccgg
      601 cgcattcggc tttgggtgtg cttttctgcc tgaagatgtt atccgttttg acactaagag
      661 tgatttcctg gcctgggtaa ggaatgcgct gccaggtgaa tattccgttg ctggccccta
      721 cgacatcatc atacccgaca cacggtttga aggggtgctc agcatccggt ggactgatgc
      781 acgccctgag acaacagaac cgcggtacag agccaaatcc cttacttttt acggcattaa
      841 cggccccatt tatcaccccc gctactgcta ctggcccata tccagactga ctgactgggt
//
LOCUS       DAJTYD010000341          897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTYD010000341 DAJTYD010000000
VERSION     DAJTYD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.346"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..897)
                     /locus_tag="OO585_004036"
                     /pseudo
     CDS             complement(1..897)
                     /locus_tag="OO585_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          235 a          233 c          198 g          231 t
ORIGIN      
        1 ccataattca gcctctggga attttggctg tagttatagc tgccgttcac cgtgccataa
       61 gagccatcat aggtcaggga catattaccg ccaacctcat catcatgact gttataggac
      121 tgcattaaat tccatgaaag gttatttctt tccagcgccg taccgcttga tgtgagctac
      181 cggaattatc gttttgcgaa acagaatagg tggcgtaact attaccccac cagcccgata
      241 aaggaacaga taaagagagc atgtagatct gctcactggt gttatcactg gcatggttgt
      301 tccccgtccc ctgtctggca gtattggaat aactccagga gacgttccac gagagacgct
      361 tccagctact gctgtagcca aattgcatta atcgttcgac atcatcggta tgccagtatt
      421 gttgccggct taatgtcagg tataaattgc cataatctct ccccaacgac tgcgttagcg
      481 ttccctccac gcgactacgt cggttacctg tttcccaaaa gtcttcaggg ctgttgcgcc
      541 gcgatgccca ctcattgagg gtatagtagc cctcggtaga gtaacgatag ccaaccaggc
      601 gcaagctagt gtctgtgtcg tcaaatcgtt ttgaatagag aaagcgatat gactgacccg
      661 tttctgtatc atcatggcta aaattcgcac gagcatgtgt cacatcgaaa gaaattgcgc
      721 ccaaagcgcc aagatcttta ccgacaccca gagctgcaga accataattt tcagcagcct
      781 gcatacctcc atataaagtg atcccgtggg aaaaaccatg aagtatttgc gcctgaagga
      841 cgtccggtgt aaatccgtct tcatcgcgca attcacccac gctgacatca acatctg
//
LOCUS       DAJTYD010000342          890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTYD010000342 DAJTYD010000000
VERSION     DAJTYD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.347"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>890
                     /gene="pdeF"
                     /locus_tag="OO585_004037"
     CDS             <1..>890
                     /gene="pdeF"
                     /locus_tag="OO585_004037"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6631426.1"
                     /translation="RENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFY
                     LRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPL
                     MMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNY
                     MAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVK
                     NYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQ
                     FRFFWDGMPMQPQIGVSYCY"
BASE COUNT          206 a          211 c          208 g          265 t
ORIGIN      
        1 cagagaaaat ctggtcggtg tcatgccttt taacctcggg actttaatca attatcaggc
       61 cttactggtg ggtaatctga tcggtgtccc gctgtgctac ttcatcattc gggtagtgcg
      121 aaatccattt tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaagt
      181 caccaaaaaa gagttcgcgc tctggctact ggcattaggt gctttattgt tgctgttatg
      241 catgccgtta aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgct
      301 gcccctgatg atgtggggag cgatgcgcta tggttataag ctgatttctc tgctctgggc
      361 ggtcgtgttg atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta
      421 taccacgcag ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta
      481 tatggctgta ctggcaaccc gtcagagagc ggtagtcaga cgcattcagc ggcttgcgta
      541 tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc
      601 cccctggtct gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaacta
      661 tggcatcatg ctgcggattc aatacaagca aaaactttct cactggctgt cacccttgct
      721 ggaaccgggt gaagatgttt atcagctttc gggtaacgat ctcgcgctgc gactgaatac
      781 agaatcgcac caggagcgca ttaccgcact ggatagccat ctcaagcaat ttcgtttctt
      841 ttgggatggc atgccgatgc aaccgcagat tggcgtcagt tactgctatg
//
LOCUS       DAJTYD010000343          889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTYD010000343 DAJTYD010000000
VERSION     DAJTYD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.348"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..672)
                     /locus_tag="OO585_004038"
     CDS             complement(<1..672)
                     /locus_tag="OO585_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6631427.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQS"
     gene            complement(665..>889)
                     /locus_tag="OO585_004039"
     CDS             complement(665..>889)
                     /locus_tag="OO585_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631428.1"
                     /translation="FINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMP
                     LHCPPQAIAHHQQLEQQALASLQHWGVFHV"
BASE COUNT          197 a          236 c          251 g          205 t
ORIGIN      
        1 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
       61 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
      121 gcgcagagta cttttgaaat caaagtttcg cgccagtgga ataaatgagc ggcgacggcg
      181 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
      241 aacaacctgg cgcacaatcc tgcgggcggc agccagtact tcggggttca tcagatgttt
      301 ggtgtgcaaa acggcgcgta gcaggctttc ggaaggctgc atacgttcca gcacgtcgag
      361 atttgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gccgctcaat
      421 cacctgttgc ggaaacagcg tgtggatact gttgaaccac tcaggagtgg tgagatttga
      481 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
      541 ccactccagc gcgtgatcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
      601 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
      661 cagttcagac atggaaaact ccccagtgtt gcagcgatgc cagtgcctgc tgttcgagtt
      721 gttgatgatg tgcaatggct tgcggtggac aatgcaacgg catttgcagt gccgaaacgg
      781 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
      841 gcggtagcca ggccatcacc gcgcgtaaat cgggcagggc attgataaa
//
LOCUS       DAJTYD010000344          876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTYD010000344 DAJTYD010000000
VERSION     DAJTYD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.349"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>876
                     /locus_tag="OO585_004040"
     CDS             <1..>876
                     /locus_tag="OO585_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR6631429.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQV"
BASE COUNT          207 a          193 c          233 g          243 t
ORIGIN      
        1 tggttgaaga gcgtcagcgc gggttaacca gtttgcgcca aattaaccgc aagacccggg
       61 aagtcattgg tattgccttt gatgatccag cctatgtgac ctggattgcc tacaatccag
      121 aacctgaaac cgcgcgattg cgttatggtt attcttccat gaccacacca gacactttgt
      181 ttgaactgga tatggatacc ggtgagcgtc gtgtattaaa acaaacggaa gttcctggtt
      241 ttgatgcggc gaattaccgc agtgaacacc tgtggatagt cgcccgtgat ggcgtcgaag
      301 ttccggtttc gttggtctac catcgcaaac attttcgcaa aggacacaac ccgttgctgg
      361 tgtatggcta tggttcttac ggcgcaagta ttgatgccga tttcagtttt agccgcttga
      421 gtttgttaga tcgtggcttt gtctacgcca ttgtccatgt tcgcggcggt ggtgagctgg
      481 ggcaacaatg gtacgaagac ggaaaatttc tgaagaagaa aaatacgttt aatgattatc
      541 ttgatgcctg cgatgcattg ttaaaactgg gctatggctc tccttcgctt tgttatgcga
      601 tgggcgggag tgcggggggc atgttgatgg gcgttgcaat taatcaacgc ccggaattat
      661 tccacggcgt tatcgcccag gtaccgtttg ttgatgttgt aacaacgatg cttgatgaat
      721 caattcctct taccactggt gagtttgaag agtggggtaa cccgcaggat ccgcaatatt
      781 acgagtacat gaaaagctac agcccgtatg acaacgtcac cgcacaggct tatccgcatt
      841 tactggtaac gaccggttta cacgattctc aggtgc
//
LOCUS       DAJTYD010000345          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTYD010000345 DAJTYD010000000
VERSION     DAJTYD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.350"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>863
                     /locus_tag="OO585_004041"
     CDS             <1..>863
                     /locus_tag="OO585_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6631430.1"
                     /translation="LINWGMPTLAAEMLNALDCQRTLPLYLQASLLPKAERGELVAKA
                     IDVFPQFVRFPNTLEEVAALESIEECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSP
                     EFADGWRGLAIHAWNKQHDYELAARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKR
                     LARLENNLEIALKRDDMTAELLNLWHLTGQADKAADILATRKFHPWEGGEGKVTSQFI
                     LNQLLRAWQHLDAREPQQASELLHAALHYPENLSEGRLPGQTDNDIWFWQAICANAQG
                     DETEAMRCLRL"
BASE COUNT          205 a          222 c          250 g          186 t
ORIGIN      
        1 aactgattaa ctggggaatg cccaccctgg cggcagagat gctgaacgca ctggactgcc
       61 agcgcacgct gccgctttac ctgcaagcca gcttgctgcc gaaagccgaa cgtggcgaac
      121 tggtcgcaaa agccattgat gtcttcccgc agtttgtccg tttcccgaat acgctggaag
      181 aagtggcggc gctggagagt attgaagagt gctggtttgc tcgccattta ctggcctgct
      241 tctactacaa caaacgtagc tacaacaaag ccattgcctt ttggcaacgt tgcgtagaga
      301 tgtcgccgga gtttgccgac ggctggcgcg ggttagcgat ccatgcgtgg aataagcaac
      361 acgattatga gctggccgcg cgttatcttg ataatgctta tcagcttgcg ccgcaggatg
      421 cacgtctgct tttcgaacgg gatttgcttg ataagttaag tggagccaca ccggagaaac
      481 gactggcgcg tctggaaaat aatctggaaa ttgcgctgaa acgcgacgac atgaccgcag
      541 aactgctcaa tttgtggcat ctcacgggtc aggcagacaa agcggcggac attctcgcca
      601 cgcgtaaatt ccacccgtgg gaaggcgggg aagggaaggt caccagtcag tttatcctca
      661 accagttatt acgcgcctgg cagcatcttg atgccagaga gccgcagcag gccagcgaac
      721 tgcttcatgc cgcgctgcat tatccggaga atttaagcga aggccgttta ccggggcaaa
      781 ctgataacga catctggttc tggcaggcga tatgcgccaa cgcgcagggc gatgaaactg
      841 aagcgatgcg ttgtttacgt ctg
//
LOCUS       DAJTYD010000346          837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTYD010000346 DAJTYD010000000
VERSION     DAJTYD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.351"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..451
                     /gene="pgrR"
                     /locus_tag="OO585_004042"
     CDS             <1..451
                     /gene="pgrR"
                     /locus_tag="OO585_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6631431.1"
                     /translation="MDKDMIAIRIGPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAIN
                     LYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERA
                     IKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
BASE COUNT          221 a          155 c          177 g          284 t
ORIGIN      
        1 gatggataaa gatatgatcg ccattcgaat cgggccagat ataccaatgg ctattgttgg
       61 ctcaccggat tatttttctc gccgaagtgt tccaacgtca gtgtcacaat taatagatca
      121 tcaggcaatt aatttgtatc ttcccacatc gggtacagca aatcgctgga gattaatacg
      181 cggtggacgt gaagttcgtg ttcgcatgga aggtcagctt ttactgaata cgatagacct
      241 gatcattgat gctgcaattg atgggcatgg attggcgtat ctaccttatg atcaggttga
      301 gcgggctatt aaagaaaaaa aactgatacg tgttttggat aaattcacac cagatttacc
      361 cggttatcac ctgtactatc cacaccgtcg acatgctggc tcggcattct cattatttat
      421 agataggctg aagtataaag gtgctgttta gcactacttg ctgatacatt aatttaattt
      481 ctctcttaac gtattctcag ttcctttcaa cgttttggtt attttttatt cttcgtacaa
      541 tggcgacaga tgctgatttt gataccgaaa actggtttga acgtgcgaag cctgaacgta
      601 gtattcggtg ttctatgtgc tttgccgcat tttggcgtcg attatttaca ctaaatctga
      661 tttaatatat tgatacataa aacattttat gcttcctgtg tcactttttt gatggaagtt
      721 gtttgcattt ctttaaggcg aaacaaataa ttacgcatca attttaatgt cggttagagg
      781 gaaacttatg aagcactctg tttcagtcac gtgttgtgcg ctgttggtca gcagcat
//
LOCUS       DAJTYD010000347          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTYD010000347 DAJTYD010000000
VERSION     DAJTYD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.352"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>829
                     /locus_tag="OO585_004043"
     CDS             <1..>829
                     /locus_tag="OO585_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR6631432.1"
                     /translation="KRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRIDRL
                     LGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGRSVT
                     LYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGWYWL
                     SRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSRSKG
                     RKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEETFR
                     "
BASE COUNT          255 a          201 c          165 g          208 t
ORIGIN      
        1 aaaacgactc aacagcttaa cgttggcttg ccacgcatta cttgactgta aaactctcac
       61 tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg
      121 aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg
      181 gcatgctagc tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga
      241 tattcgtgag caaaaacgac ttatggtatt gcgagcttca gtcgcactac acggtcgttc
      301 tgttactctt tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga
      361 ccaatttcta gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag
      421 tgatgctggc tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt
      481 aagtcgagta agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat
      541 cagcaactta catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac
      601 taaaagcaat ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa
      661 aaatcagcgc tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc
      721 ggcaaaggag ccatgggttc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca
      781 acttgttaat atctattcga agcgaatgca gattgaagaa accttccga
//
LOCUS       DAJTYD010000348          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTYD010000348 DAJTYD010000000
VERSION     DAJTYD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.353"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..567
                     /gene="ospC4"
                     /locus_tag="OO585_004044"
     CDS             <1..567
                     /gene="ospC4"
                     /locus_tag="OO585_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6631433.1"
                     /translation="KDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKK
                     IKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQ
                     DVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTML
                     DNAMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          295 a          132 c          161 g          241 t
ORIGIN      
        1 aaagatcaag gattcagaga gttttgttat aacaaaaata ttgatcctgt tagtctggat
       61 agaattataa actttgtgtt tcagcttgag tatcatatac caagaatgct aagtacagat
      121 aacttcaaaa aaataaaact cagagacata tctttggagg atgcgattaa agcatctaat
      181 tatgaagaga ttaacaataa agtgactgat aaaaaaatgg ctcatcaggc ccttgcttat
      241 tctttaggca ataaaaaagc tgatatagca ctttatttac tatctaaatt taattttaca
      301 aaacaagatg tcgcagagat ggagaaaatg aacaacaata gatattgtaa tctgtatgat
      361 gttgaatact tacttagtaa agatggtgcc aactataaag tgctagaata ttttatcaat
      421 aatggattgg ttgatgtaaa caaaaaattt caaaaagcaa atagtgggga cactatgctt
      481 gacaatgcaa tgaaaagcaa aaatccaaaa atgattgatt ttttattaaa aaatggagca
      541 atattaggca aacgatttga gatatgacct aaaagctcgg ccaaataata atgtcgtcga
      601 ttatcgaagt catcatgagt gattctgtcc ccacagtgca gcatcagcat gacagtgaat
      661 tgtctggcgt ggtccattta atgagatgga gatggtccct ctttcctaaa tagctgtgcc
      721 gaataccgct acacttttgc cagcccatgt ttgcctccgg ggaatgggct gacggttcct
      781 tcaaaatcca ttgcacgcag ttatgtcgga gcgggaactt gactcctgt
//
LOCUS       DAJTYD010000349          806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTYD010000349 DAJTYD010000000
VERSION     DAJTYD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.354"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..356)
                     /locus_tag="OO585_004045"
     CDS             complement(<1..356)
                     /locus_tag="OO585_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HCR6631434.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENG"
     gene            488..>806
                     /locus_tag="OO585_004046"
     CDS             488..>806
                     /locus_tag="OO585_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding ATPase"
                     /protein_id="HCR6631435.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGV"
BASE COUNT          208 a          189 c          211 g          198 t
ORIGIN      
        1 aaaccgtttt ctttcaggcg agagtagata agttctgaca actggcggtt ctcttcgctg
       61 tgtgtcgagt gatagttgtc atagctgatg ttaaagcctg cgaaatcagt ctgatgctcc
      121 tgactcattt cgccaatcat ctgctccggg gtgataccaa gctgctgagc tttcagcatg
      181 atcggtgtac cgtgggcatc gtcggcgcag atgaagttga cctcgtggcc gcgcattcgc
      241 tggtaacgga cccagacatc agcctggatg tgctccagca tatggccgag gtggattgag
      301 ccgttagcgt acggcagtgc gcacgtcacc agaattttct tcgcgacttg agtcatagta
      361 ggcattactt cttaatagtg aaaaggggtt agatagtacc aaatgggaaa atgttaagta
      421 agtacttctc tatattcttc aggtaaacta tcagctgatg gtttatctat acaaaaaagg
      481 agtcgggatg aacgaacaat cccaggccaa atcaccagaa gccttgcgcg caatggtcgc
      541 cgggacactg gccaattttc agcacccaac cctgaagcat aacctcacca cgctgaaagc
      601 gttgcaccat gtcgcctgga tggacgacac gttgcatgtg gaactggtta tgccattcgt
      661 ctggcatagc gcgtttgaag agttaaaaga gcaatgtagc gccgaactgc tgcgtatcac
      721 cggggcgaaa gctatcgact ggaaactgtc gcacaatatc gccaccttaa aacgcgtgaa
      781 gaatcagccg ggtattaatg gtgtga
//
LOCUS       DAJTYD010000350          800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTYD010000350 DAJTYD010000000
VERSION     DAJTYD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.355"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..420
                     /locus_tag="OO585_004047"
     CDS             <1..420
                     /locus_tag="OO585_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6631436.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            436..>800
                     /locus_tag="OO585_004048"
     CDS             436..>800
                     /locus_tag="OO585_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6631437.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQ"
BASE COUNT          230 a          190 c          190 g          190 t
ORIGIN      
        1 ttgatgaaca attccgatgc ccgaaaaacg tcggcgttac tatttcttga tggcgataat
       61 ttcaaatata tcaatgatac ctggggtcat gcgacgggcg atagggtctt gattgaaatc
      121 gcaaaacggt tagctgaatt tggcgggctg cgacataaag cataccgcct gggcggcgat
      181 gaattcgcta tggtgctcta tgatgtacag tcggaatctg aagtgcaaca gatatgctca
      241 gcactgacac aaatctttaa tctcccgttt gatcttcata atggccatca gaccaccatg
      301 acattaagca ttggttacgc gatgaccatt gagcacgcct ctgcggaaaa attacaagag
      361 cttgccgatc acaatatgta tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa
      421 caaggatata tcgatatgat aaagcacctg gtagcccccc tgattttcac ctcactaata
      481 ctgactggct gccagtcccc tcagggaaag tttactcctg agcaagtcgc cgctatgcaa
      541 tcttatggat ttactgaatc cgccggcgac tggtcgctgg gcttatcaga tgccattctg
      601 ttcgcaaaaa atgactacaa attgctcccg gaaagccagc aacagatcca aaccatggca
      661 gccaaattgg cctcgacagg gctaacacat gcccgtatgg atggacacac cgataactat
      721 ggtgaagaca gttacaacga aggcttatca ttgaaacggg cgaatgtcgt ggccgatgca
      781 tgggctattg gtggacaaat
//
LOCUS       DAJTYD010000351          799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTYD010000351 DAJTYD010000000
VERSION     DAJTYD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.356"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..148
                     /locus_tag="OO585_004049"
     CDS             <1..148
                     /locus_tag="OO585_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6631438.1"
                     /translation="AILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQ
                     ALFS"
     gene            241..>799
                     /locus_tag="OO585_004050"
     CDS             241..>799
                     /locus_tag="OO585_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6631439.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSV"
BASE COUNT          209 a          187 c          227 g          176 t
ORIGIN      
        1 ggctattctc gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat
       61 caatctggta gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat
      121 tgctcatgca caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg
      181 ctaagcttgc ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac
      241 gtgaagttca aagttatcgc cctagcggca ttaatgggta ttagcgggat ggcagcgcag
      301 gctaacgaat tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg
      361 gtgccagaca ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca
      421 gccaagaaac aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag
      481 atcgcgaaaa aagatatcag ctcagcgaat ttacgcaccc agccagatta tgattatcag
      541 gatggtaaaa gtatcctgaa aggctaccgc gctgtgagaa cggtggaagt cacgctccgt
      601 cagttagaca aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt
      661 cgttcagtgt cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca
      721 gcgattgata acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg
      781 gggccggtat atagcgtgc
//
LOCUS       DAJTYD010000352          793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTYD010000352 DAJTYD010000000
VERSION     DAJTYD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.357"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            13..793
                     /locus_tag="OO585_004051"
                     /pseudo
     CDS             13..793
                     /locus_tag="OO585_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          240 a          162 c          163 g          228 t
ORIGIN      
        1 aggaagcata acgtgactat aaaaccaact aaaaattatc ataatcatct gacccgtatc
       61 gctacgtttt gcgcgctgct gtattgcaat agtgctttct gtgctgaact tgttgaatat
      121 gaccatacct tcctgatggg gcagaatgcc tcaaatattg atctcagccg gtatagtgaa
      181 ggtaaccctg ctataccggg tatgtacgac gtcagtgttt atgtcaacga tcaacctatt
      241 attaaccaaa gcattacatt tattgaaatt gaaggaaaaa aaacgcacaa gcctgtatca
      301 cacttaagaa tttattgcag tttcatataa attctcccga tataaataac gaaaaagccg
      361 ttctgcttgc cagggatgaa acactcggca attgccttaa tttgacggaa attatccctc
      421 aggcttctgt tcgttatgac gttaacgagc aacgtctgga tatagacgtt cctcaggcat
      481 gggtaatgaa aaattaccaa aactatgttg atccatcgtt atgggaaaat ggcattaatg
      541 cggccatgtt gtcatacaac ctcaacggat atcatagtga aactcctggt cgaagaaatg
      601 acagcattta tgctgctttt aacggtggga tgaatttagg tgcatggcga ctgcgtgcct
      661 caggcaacta caactggatg accgattctg gcagtaatta tgattttaag aatcggtata
      721 tacagcgtga tatcgcctcg ctgcgttctc aactcattct tggtgagtct tatacaacag
      781 gcgaaacctt tga
//
LOCUS       DAJTYD010000353          789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTYD010000353 DAJTYD010000000
VERSION     DAJTYD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.358"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..672
                     /locus_tag="OO585_004052"
     CDS             <1..672
                     /locus_tag="OO585_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6631440.1"
                     /translation="RSLLGADEQAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYL
                     MALGFEEAVLLETAADLRFAPEFVARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLL
                     AEMLGWTCFTQVERFTLDALFITLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGM
                     RQRMAAGKAEIIRKTVAAEMPAMQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLR
                     QRMQP"
     gene            669..>789
                     /locus_tag="OO585_004053"
     CDS             669..>789
                     /locus_tag="OO585_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6631441.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVV"
BASE COUNT          183 a          188 c          248 g          170 t
ORIGIN      
        1 cgaagtttac tcggtgctga tgaacaggcc gctgccgcgc tgctgttggc gcagagaaaa
       61 aacggtacgc cgatgtcttt aaccgcgttg agcatggggg atgaacgggc attgcactgg
      121 ctgcgctatc tcatggctct tgggtttgag gaagctgtat tgctggagac ggcggcagat
      181 ctgcgctttg cgccggaatt tgtcgctcgc catattgccg aatggcagca tcagaatccg
      241 ctggatctga taattaccgg cagccaaagc agcgaagggc agaatgggca aacgccattt
      301 ttgctggcgg aaatgctggg ctggacctgc ttcacccagg tggagcgttt cactctcgac
      361 gcgctgttta tcaccctcga acagcgtact gaacatgggt tgcgttgttg ccgggttcgc
      421 ttgcctgcgg tgattgccgt gcgtcagtgc ggtgaagtgg ccttgccagt accgggaatg
      481 cgccagcgaa tggctgcggg gaaagcagaa attatccgta aaactgttgc tgccgaaatg
      541 ccagcgatgc agtgtctgca actggcaaga gcagagcaac gacgcggagc gacgctgatt
      601 gatgggcaaa cggtagcaga aaaagctcaa aagctatggc aggactattt acgccaaagg
      661 atgcagccat gaatatcgca attgtgacaa taaatcagga gaatgccgca atagcaagct
      721 ggctggcggc gcaggatttc agtggttgta ccctggcgca ctggcagatt gaacctcagc
      781 ctgtagtgg
//
LOCUS       DAJTYD010000354          776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTYD010000354 DAJTYD010000000
VERSION     DAJTYD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.359"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(14..604)
                     /locus_tag="OO585_004054"
     CDS             complement(14..604)
                     /locus_tag="OO585_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6631442.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            complement(604..762)
                     /locus_tag="OO585_004055"
     CDS             complement(604..762)
                     /locus_tag="OO585_004055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631443.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
BASE COUNT          186 a          232 c          184 g          174 t
ORIGIN      
        1 cgcagtcagc gtatcaatcc tgtgcatctt catcctcatc tggataggaa aataaaacga
       61 cggtgtgtga cggacataat ttattaatca cgcactccgc aaccgtatcc ccccacgtcc
      121 ggatcggtgt gttgcaggcg tccgtgcatg tctgccactg agcaccggca tccaccggca
      181 acgtcacacg ccagaaataa cgccagcgat ctccccattc cggatcgggg cttgcatcca
      241 ggtgctggaa ctgttcgatc gttacgccgg tatatcccag cgcctcaagc tgatccagga
      301 agaactgctc atttatgcca ccagccacat tggcttttgc ttcaagacgt tgctgacgct
      361 ggcgtaatgt ctgggttccg acaggggaac aggaatcagg taaaccatac aattcttcat
      421 aacgttcaat cagctctgtg gactgaccgg gatcgatttc aatcaccagt tcatcagccc
      481 gctggtgaac acgcacaagc gatggtgcca gaccgtcaag tacaccgtcg gtatctgacc
      541 atgcaggccc cggctgcatc agtccgtaca gcagctttgt ataatcatcc tgtaacgaat
      601 ccattattta ctccttgccg ggtcataagc ctgccaggtg atctcgccga gcaccggaag
      661 ctcggtctcc cccaggtcaa tatccgatga agggacgatt aaccggtggg ccacttcacc
      721 agcagacaaa ctgatggcct cactgattct ggacagatac atacgcccct ctggca
//
LOCUS       DAJTYD010000355          766 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTYD010000355 DAJTYD010000000
VERSION     DAJTYD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.360"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            177..>766
                     /gene="ruvA"
                     /locus_tag="OO585_004056"
     CDS             177..>766
                     /gene="ruvA"
                     /locus_tag="OO585_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6631444.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIR"
BASE COUNT          203 a          184 c          189 g          190 t
ORIGIN      
        1 ctgaatacgt aatatttaaa tatttgcttc caatataacc tgtagaataa atcatactgc
       61 gccatttttc agttcatcga gacacctcgc aagttttctt catccttcgc tggatatcta
      121 tccagcattt ttttatcata cagcattatc tttgattcat tacgcaggag cgtcatgtga
      181 taggcagact cagaggcatc atcattgaaa aacaaccccc gctggtgtta attgaagtgg
      241 gcggcgtagg ctatgaagtg catatgccga tgacctgttt ttatgaactc cctgaagcgg
      301 gtcaggaagc gatcgttttc acccactttg tggtgcgtga agacgcgcaa ctgctgtacg
      361 gttttaacaa taaacaagag cgcacattgt tcaaagagtt gatcaaaacc aacggcgtcg
      421 gcccgaagtt ggcgctggcg atcctctccg gaatgtcagc gcagcagttc gttaatgccg
      481 ttgaacgtga agaagtgggg gcactggtga aactgccggg tattggcaaa aaaaccgccg
      541 aacgcttgat tgttgaaatg aaagaccgat ttaaaggttt gcatggcgat ctgtttaccc
      601 ctgccgccga cctggtactc acatcacctg ccagcccggc gaccgacgat gctgaacagg
      661 aagcggttgc cgcgctggtg gcgctgggct ataaaccaca agaagcaagc cgcatggtga
      721 gcaaaatcgc tcgccctgac gccagcagtg aaacattaat tcgcga
//
LOCUS       DAJTYD010000356          764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTYD010000356 DAJTYD010000000
VERSION     DAJTYD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.361"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..648
                     /gene="ospC2"
                     /locus_tag="OO585_004057"
     CDS             <1..648
                     /gene="ospC2"
                     /locus_tag="OO585_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6631445.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFINNGLVDVNKRFQKANSGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
BASE COUNT          279 a           99 c          151 g          235 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattaa taatggattg gttgatgtaa acaaaagatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aagattcaaa aacaattgat
      601 tttttattaa gaaatggagc ggtatcaggc aaacgatttg ggaggtgacc taaaatatat
      661 ttaggttgtg ccctgaatct gacgttgtca tttttgttat attgatcgat ataggggatg
      721 gtgcagaccg ttcccctgca acaacacatt gcatgtggtg aacg
//
LOCUS       DAJTYD010000357          744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTYD010000357 DAJTYD010000000
VERSION     DAJTYD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.362"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..>744
                     /locus_tag="OO585_004058"
     CDS             1..>744
                     /locus_tag="OO585_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6631446.1"
                     /translation="MPNDPKWRTIARVSGQPIATVMAVYIHLLVSASRNVTRGHIDVT
                     TEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGNISQTAKSPAER
                     KRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQNTMVHGVKNATN
                     QAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQLQQPEADSAIQR
                     EADRVVPENTGQSVGRVDYPDVFEQVWREY"
BASE COUNT          220 a          175 c          222 g          127 t
ORIGIN      
        1 atgccaaatg accctaagtg gcgaacaatt gccagggtgt cagggcagcc aattgcaaca
       61 gtgatggcag tgtatatcca cctcctggtg agcgcgtcac gaaatgtcac gcgaggtcac
      121 attgatgtca cgacagaaga tttggcaagt gcgctcgacg tgacagaaga ggtaattgat
      181 tcaattttgc agacgatgca ggggcgggta cttgatggtg atttaatcac tggatgggaa
      241 aaacgccagg tgctgaaaga ggacaacggc aatatttcgc aaaccgcaaa atctcctgca
      301 gagcgcaaga gggcgcagcg agagagggaa agaaagcggg aacaaaatgg cgattgtcac
      361 ggcgcgtcac gatatgtcac gcacatgtca cgacgagtca cgacagataa agatacagat
      421 aaagatacag atcaagaaga tcaaaacact atggtccatg gcgtaaaaaa cgccacgaac
      481 caggcagggg atgttcagac cgtcaatcct ggtcagccag caggcacgac accggaagcc
      541 gattcagcgt atgcgctgaa agccgattcg ggcgctgtgc agcaggcgat gaccgcaagt
      601 ccggagcaat cacaccaact gcagcagcct gaagccgatt ccgccattca gcgggaagcc
      661 gatcgggtag tcccggaaaa caccgggcag tctgtgggac gagtggatta tccggatgtg
      721 ttcgaacagg tctggcggga gtac
//
LOCUS       DAJTYD010000358          736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTYD010000358 DAJTYD010000000
VERSION     DAJTYD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.363"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..392
                     /locus_tag="OO585_004059"
     CDS             <1..392
                     /locus_tag="OO585_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6631447.1"
                     /translation="HYPLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEI
                     DEKFMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKPEHPLPSI
                     KLYISYNKSSLNNLVFSRFIDRLNESF"
     gene            539..>736
                     /locus_tag="OO585_004060"
     CDS             539..>736
                     /locus_tag="OO585_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6631448.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWE"
BASE COUNT          228 a          154 c          118 g          236 t
ORIGIN      
        1 gtcattaccc tctaatttgt agtaataagc atccgcgaat tactgacaat agcacttatg
       61 aacaaattat ggcagaagag ttcacccaat taatatccaa atcagcagga gttgatgata
      121 ttcaaatgga aatagacgag aagtttatga atcgcaaaat ttcatttcgt ggttcctctt
      181 tgctgacaat aattaatagc attgcagtta cagatttact gggaatagtc ccctatgaac
      241 tttataactc ccaccgtgac tttcttaatt taaaagagat aaaaccagaa catccgttac
      301 cttcaataaa gctttacatt tcctataata aatcttcttt aaataatttg gtgttctccc
      361 gatttattga ccgtctaaat gagagttttt gatatataac tacagacagc tactataact
      421 ttcatctatt tattcacagc gcgaatgaaa aataaaacac tctgctttac agtgtttact
      481 ttataattaa cagtcttatc tcactgttca ccctgtaacg ttcagagagc gtcttcagat
      541 gtctatttat aaaattcctc ttccgctcaa tattctggaa gctgcacgag agcgtattac
      601 ctggacactc aacaccctac cccgcgtttg tgtctcattt tctggcggca aagactctgg
      661 tttaatgctg catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt
      721 gtttatcgac tgggag
//
LOCUS       DAJTYD010000359          729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTYD010000359 DAJTYD010000000
VERSION     DAJTYD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.364"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..726)
                     /locus_tag="OO585_004061"
     CDS             complement(<1..726)
                     /locus_tag="OO585_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6631449.1"
                     /translation="MEELAAVKEVLESGWITTGPKNQALEQAFCQLTGNQHAIAVSSA
                     TAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDVDRDTLMVTPEAIE
                     SAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVGTYYKGRHIGAKGT
                     AIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDRQTWGRAPQAEVLT
                     PGYKYNLTDINAAIALTQLAKLEH"
BASE COUNT          166 a          191 c          187 g          185 t
ORIGIN      
        1 gtgctctaat tttgctaact gtgtcagggc aattgcggcg ttaatatcgg tcagattgta
       61 cttatagccc ggtgttaaga cttcagcctg cggtgctcgg ccccaggttt gtctgtcgta
      121 ggcatcgaca cccaggccgt gaaatttcag catccgtagc tggcgggcaa ggttttcatt
      181 atcagttaca atcaggccac cttccgcaca ggtaatattt ttaatggcat gaaatgaaaa
      241 aatagcggta ccttttgcgc caatatgtcg ccctttgtaa tacgtaccga cggcatgggc
      301 agcatcttcg ataactgcga tgccgtaacg ttcgccaatg gcgcgaatgg cgtcaatatc
      361 tgctggcgca ccggcataat gcaccggaat gatggcttta gtgcgtggcg taatggctga
      421 ctcgatcgct tcaggcgtga ccatcagcgt atcgcggtcg acatccacca ttaccggcgt
      481 tgcacccaac aaggaaatca tattgagggt tgaaacccag gtcagggaag gcgtaatcac
      541 ttcatcgccc ttgccaattt ccaacgccat tagcgtgata tgcattccgg cggtggctga
      601 actgaccgcg atggcatgct gatttcccgt caactggcaa aaggcttgct caagcgcctg
      661 attcttcgga ccggttgtga tccaaccgga ttcgagaact tctttaactg cagcgagttc
      721 ctccacgcc
//
LOCUS       DAJTYD010000360          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTYD010000360 DAJTYD010000000
VERSION     DAJTYD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.365"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            35..726
                     /locus_tag="OO585_004062"
                     /pseudo
     CDS             35..726
                     /locus_tag="OO585_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          170 a          181 c          200 g          175 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcacgtc ttcggtgaac gcactctggc cacactggag
      481 cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tggctggccg
      541 ctgtatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc
      601 attgagcgac ataatctgaa tctgagacaa catctggcaa ggctgggacg gaagtcactg
      661 tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJTYD010000361          726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTYD010000361 DAJTYD010000000
VERSION     DAJTYD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.366"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..526
                     /locus_tag="OO585_004063"
     CDS             <1..526
                     /locus_tag="OO585_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6631450.1"
                     /translation="LPWLSEADRRLQIKSALPWWLVCRGEIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVTGFYRVPLSSAPPGDI
                     LLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAF
                     TGICNDLAAASACM"
     gene            424..>726
                     /locus_tag="OO585_004064"
     CDS             424..>726
                     /locus_tag="OO585_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6631451.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGG"
BASE COUNT          144 a          202 c          229 g          151 t
ORIGIN      
        1 gctgccctgg ctgagcgagg ctgaccggcg gctgcagata aaaagcgcac tgccctggtg
       61 gctggtctgc cggggggaaa ttcataaatt ccgctgtgtg ccacatctga caggacggcg
      121 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcatacc atctggcggg
      181 aattgatatg ccggattttc atcgcgagga tgactggtgg cgcaacggcc agaacctgta
      241 cctggacaat atggcggtca ccggctttta ccgggtgccc ctgtcctctg caccgccggg
      301 cgatattctg ctgtgctgct ttggtgcttc ggtaccgaac catgccacca tttactgcgg
      361 caacggtgag ctgcttcacc atctgcctga acaactgagt aaacgggaga ggtattccga
      421 aaaatggcaa cgacgaacgc attctgtctg gcgtcaccgc cactggcacg catctgcctt
      481 cacggggatt tgcaacgatt tggccgccgc ctcagcctgt atgtgaacac ggcagcggaa
      541 gccattcgcg ccctgtcgat gcagatgccg ggctttcgcc gtcagatgaa cgaaggctgg
      601 taccagatac gtattgccgg tgatgacacg gcaccggagg cggtgtatgc ccgtcttcac
      661 gaacagctgg gtgagggaac ggtcatccac attgtgccgc gactggccgg ggccggaaag
      721 ggtgga
//
LOCUS       DAJTYD010000362          713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTYD010000362 DAJTYD010000000
VERSION     DAJTYD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.367"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..121
                     /locus_tag="OO585_004065"
     CDS             <1..121
                     /locus_tag="OO585_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6631452.1"
                     /translation="SDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(171..>713)
                     /locus_tag="OO585_004066"
     CDS             complement(171..>713)
                     /locus_tag="OO585_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6631453.1"
                     /translation="HYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFS
                     APGSFHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIP
                     HCWSEQERRFRGFLITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHP
                     AEVIAGLTGYEAFMELLHKG"
BASE COUNT          188 a          160 c          175 g          190 t
ORIGIN      
        1 ctctgactgg gataaagagc aacttcagga cgcaaactca agctggatgg tcgaagactc
       61 tttcccgaga gcattacgcg agtacaacga aatggttgat gactataata gcctgcgtta
      121 aacgtttttg atgataaatg cagtgtcgga tgcggcgcat ctgacacagt tcaccccttg
      181 tgaagtaact ccataaatgc ctcatatcct gttaacccgg caataacctc tgccggatgc
      241 tgacacaata tgcttgccag atagctaaat atttttagct catgacgcgc atcggcggcg
      301 gcgcaggcga tcaagacatg gttaatgact tcgttattca cctcaactgg ttgggcgagg
      361 gtaataagaa atccacgaaa acgtcgctcc tgttcgctcc agcaatgtgg gatggcgagg
      421 cgattaacaa tcaggttttc accttcgcct tcgcgggcga tgatgcgttg tgcttcatcg
      481 gcggtaatat ggtgttgtgc gactaattgc gcacaaattt gccgggtaat atgttgccag
      541 ctttcaccgc gtacattcga ataatgaaaa cttccggggg cagagaaaaa acgctccggt
      601 tgctggcgaa taaatgccgt cgccagaaaa tgttttattt gttcgatacc ggcagccgta
      661 atgatatttt ttacggtgat gtaattattt accgcgtctt ccagtaaata atg
//
LOCUS       DAJTYD010000363          707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTYD010000363 DAJTYD010000000
VERSION     DAJTYD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.368"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            113..>707
                     /locus_tag="OO585_004067"
     CDS             113..>707
                     /locus_tag="OO585_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCR6631454.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHDTLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPT"
BASE COUNT          215 a          126 c          174 g          192 t
ORIGIN      
        1 tatgaatctt gttgctgcag ataagcattt tgtgattgaa gtggtcaaat ctctcaaatg
       61 cagtaaaatg cagtgcgcta taattcaata acggagggag taaggagaag tcatgaaaga
      121 tcaagatgtt aggttcgcgg tgcatcataa gcttttgaaa gaatcgcatt tagatccaga
      181 ctgccttgtg gtcgatgaat tttccatatc ccttggcgcc agtagagcag acattgctgt
      241 aataaatggt gttatacacg ggtacgagct caaaagtgaa tatgactctt tggagcgttt
      301 gcctcttcaa atcaagcatt attcttctgt aatggacaag gttactcttg tcgtagctga
      361 gaaacatctt gagggagcat taaagttaat cccaggttgg tggggcgtta aaacggtttc
      421 tgttgggcca aaaggcgcca ttcttataaa gcacatgcgt ggagaaaagc ttaatcgaaa
      481 ccatgacaca ttgatgctcg ctcaattgct ttggaaagat gaatgtatcg acgtacttga
      541 acgatggggc tattccaaag gaatcaaaag caagccccga tttgagttat ggaatattat
      601 tgcggaaaat attccaatag cgaatctcag gcttgaagtc agaacagcct taaagaaacg
      661 cgtaggctgg aaagttaagg cttggcaggc tgagtctgcg ccaacca
//
LOCUS       DAJTYD010000364          707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTYD010000364 DAJTYD010000000
VERSION     DAJTYD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.369"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..375)
                     /locus_tag="OO585_004068"
     CDS             complement(<1..375)
                     /locus_tag="OO585_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR6631455.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSA"
BASE COUNT          191 a          165 c          169 g          182 t
ORIGIN      
        1 tgcagagtta ccggaggtgg agatccccag cagtacatcg ccttcgcgac ctaccgcttc
       61 aacgtagcgg gagaaaatat cattgaaacc gaaatcatta ccgacgcagg aaatatgact
      121 aacgtcagaa atagcaatcg ccgggtagcc cggacggttt tcacggtagc gaccggtcaa
      181 ctcttcggca aagtgcatag cgtcgcaatg ggaaccgccg ttgccgcagg aaagcacttt
      241 gccaccggct ttaaagctgt ctgctaacag gaccgccgcg cgctgaatgg cgtgaatatt
      301 ggcgtcatct tttaaaaagt tagccagcgt ttccgccgct tcgttcagtt cgttacgaat
      361 aagatcttgg tacatgagga tatccttcag cataaatgta atagacaaaa tgcagtgtac
      421 cggataccgc caaaagcgag aagtacgggc aggtgctatg accaggactt tttgacctga
      481 agtgcggata aaaacagcaa caatgtgagc tttgttgtaa ttatattgta aatacattgc
      541 taaatgtatt tacatccact acaaccatat catcacaagt ggtcagacct cctacaagta
      601 aggggctttt cgttatgatg attttgagta ttctcgctac ggttgtcctg ctcggcgcgt
      661 tgttctatca ccgcgtgagc ttatttatca gcagtctgat tttgctc
//
LOCUS       DAJTYD010000365          675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTYD010000365 DAJTYD010000000
VERSION     DAJTYD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.370"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..594
                     /locus_tag="OO585_004069"
                     /pseudo
     CDS             1..594
                     /locus_tag="OO585_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            599..>675
                     /locus_tag="OO585_004070"
     CDS             599..>675
                     /locus_tag="OO585_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6631456.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVV"
BASE COUNT          149 a          163 c          218 g          145 t
ORIGIN      
        1 attttttctg taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt
       61 atcagccgta tcccattcag ggggagtggt tttgaactga atggcaaagg caccagtacg
      121 cgccccacgc tgacggtttc taacctgtac ggtatggtcg ccgggatggc ggaagatctg
      181 cagagtctgg tcggcggaac ggtggtccgg cgtaaggttt acgcccgttt tctggatgcg
      241 gtgaacttcg tcaacggaaa cagtgacgcc gatccggagc aggaggtgat cagccgctgg
      301 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
      361 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
      421 acctaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
      481 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
      541 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
      601 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
      661 tggcttcgtg gtgag
//
LOCUS       DAJTYD010000366          674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTYD010000366 DAJTYD010000000
VERSION     DAJTYD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.371"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            335..>674
                     /locus_tag="OO585_004071"
     CDS             335..>674
                     /locus_tag="OO585_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631457.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQAT"
BASE COUNT          213 a          106 c          136 g          219 t
ORIGIN      
        1 gttctttttt tgttaacatt gataacaatt cggtcagtct gtcggagaga caagaaaatg
       61 ccaaatataa attttgtgta tctttttctg aaaataaact aaagaaaggg ctttatatat
      121 cacgcatatt tatttgttgg atagtcatta gatgttgaat caatgaatta ctcgttcagt
      181 taatatttaa atattcatga aatctataaa ttaaagattt gtcacttatt ggatttagta
      241 tgcttgattt aaatgacggt caattattgt tattattaag tctaatgtca tttaaaggca
      301 ttatcatatt tacgaagaga tcaggatgat gacgatgaaa aaaagtgtat tgacggcgtt
      361 tataactgtg gtatgtgcaa cgtccagcgt tatggcagct gatgataatg ctatcacgga
      421 tggcaaagta acatttaatg gtaaagttat tgctccagct tgtaccctgg tagctgcgac
      481 gaaagattca gtagtcacat tgccaaatgt cagcgccacc aagttgcaaa ctaatggagc
      541 agtctctggc gttaaaactg atgtaccaat tgccttagaa ggctgtgatg ttactgtaac
      601 taaaaatgcg acgtttacct ttagtggtac ggctgacggc gtgcaaccta cagcatttgc
      661 caaccaggct acca
//
LOCUS       DAJTYD010000367          671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTYD010000367 DAJTYD010000000
VERSION     DAJTYD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.372"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            193..462
                     /locus_tag="OO585_004072"
     CDS             193..462
                     /locus_tag="OO585_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631458.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..671
                     /locus_tag="OO585_004073"
                     /pseudo
     CDS             459..671
                     /locus_tag="OO585_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          166 a          154 c          197 g          154 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt g
//
LOCUS       DAJTYD010000368          657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTYD010000368 DAJTYD010000000
VERSION     DAJTYD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.373"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..371)
                     /locus_tag="OO585_004074"
     CDS             complement(<1..371)
                     /locus_tag="OO585_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6631459.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGA"
     gene            complement(468..>657)
                     /locus_tag="OO585_004075"
     CDS             complement(468..>657)
                     /locus_tag="OO585_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCR6631460.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT          192 a          181 c          139 g          145 t
ORIGIN      
        1 aacgcgccag cgatactgac attcttccag aaattaatca tatttggccc aaccgcatcg
       61 ccggtcatat cccagtaatg gtgaccaatc accgccgtac ccagcgtgta gaaaataaac
      121 agcaccgcca gcggacgggt gaaaaagcca agcacgatta atatcgcggc gggcacttcc
      181 ataactaccg caataatcgc tgccagcatc ggcattggcg cgcccaacga ggccatatat
      241 tggaccgtac cgtcaaagcc catcattttg ggaaaaccca aaataatgaa aattaagacc
      301 acggcgatac gggcaattaa taacaaaacg gggcgggcag ctccaaaatc aaaataacgt
      361 agtgtgttca taaccaatcc ctttgcaaga taatgtgatt taaacgtaat acacactctt
      421 gcacctcgcc acgccccagg cgttgagata ttcgctaatt atcctggtta cagtgattta
      481 agcgcgatgt tctcatctat cagcagtttc gcatcaaacg ttttgccgct ctggatccat
      541 ttgcgaattg ggcgaatctc acgcccctga ttcagcgtga ccgcaccgat aagcacgccg
      601 ttttgcagat taaaccaaat cgccttctga gtttccgggt tgccacgaca aagccag
//
LOCUS       DAJTYD010000369          650 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTYD010000369 DAJTYD010000000
VERSION     DAJTYD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.374"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>650
                     /locus_tag="OO585_004076"
     CDS             <1..>650
                     /locus_tag="OO585_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6631461.1"
                     /translation="ATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRL
                     TEKTDLIPEGGIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVAK
                     RVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVET
                     ATGELAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRR"
BASE COUNT          144 a          199 c          210 g           97 t
ORIGIN      
        1 gccacagacc cggcaggtaa ccgcctgccc gacccggagc tgcacccgga cagcaccctc
       61 agcatgtggc cggataaccg tatcgcccgt gacgcgcact atctttaccg gtatgaccgt
      121 cacggcaggc tgacagagaa aaccgacctc atcccggaag ggggtatccg cacggatgat
      181 gagcgcaccc accggtacca ttacgacagt cagcaccggc tggtgcacta cacgcggaca
      241 caatatgcag agccgctggt cgaaagtcgc tatctttacg acccgctggg ccgcagggtg
      301 gcaaaacggg tgtggcggcg tgaacgtgac ctgacgggct ggatgtcgct gtcacggaaa
      361 ccggaagtga cctggtacgg ctgggacggc gaccggctga ccacaataca gaacgacagg
      421 agccgcatcc agacgattta tcagccgggg agcttcacgc cactcatcag agttgaaacc
      481 gccaccggtg agctggcgaa aacgcagcgc cgcagcctgg cggataccct tcagcagtcc
      541 ggcggcgaag acggtggcag tgtggtgttc ccgccggtgc tggtgcagat gctcgaccgg
      601 ctggaaagtg aaatcctggc tgaccgggtg agtgaggaaa gccgccgctg
//
LOCUS       DAJTYD010000370          638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTYD010000370 DAJTYD010000000
VERSION     DAJTYD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.375"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>638
                     /gene="mppA"
                     /locus_tag="OO585_004077"
     CDS             <1..>638
                     /gene="mppA"
                     /locus_tag="OO585_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6631462.1"
                     /translation="IKDEPASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVAT
                     QWKSNDNRIWTFTLRDNAKWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINN
                     AQAIIDGKATPDQLGVTAVDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEW
                     TKPGNLIGNGAYVLKERVVNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESA"
BASE COUNT          175 a          152 c          168 g          143 t
ORIGIN      
        1 acattaaaga tgagcctgcg tcgctggatc ccgctaaagc cgtgggcctg ccagagattc
       61 aggtcattcg cgatctgttt gaaggactgg tgaatcagaa cgagaaaggg gagattgtcc
      121 ccggcgttgc gactcagtgg aaaagtaatg acaaccgtat ctggactttt accctgcgcg
      181 ataacgcaaa atgggcggat ggcacaccgg taacggcgca agattttgtc tacagctggc
      241 agcgtctggt ggacccgaaa acgctatcgc catttgcatg gtttgccgcg ctggcgggaa
      301 tcaacaacgc acaggcgatt attgatggta aagctacgcc tgaccagctt ggcgtcaccg
      361 cagttgatgc ccataccttg aaaattcagc ttgataaacc gttgccgtgg tttgtgaatt
      421 taaccgctaa ctttgccttc ttcccggtgc aaaaagccaa cgtagaaagc ggtaaagagt
      481 ggacgaaacc cggaaatctg atcggcaatg gcgcttatgt tcttaaagaa cgcgtagtca
      541 atgaaaaact ggtcgtggta ccgaataccc attattggga taacgccaaa acggtactgc
      601 aaaaagtgac cttcctgcca attaatcagg aatccgca
//
LOCUS       DAJTYD010000371          621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTYD010000371 DAJTYD010000000
VERSION     DAJTYD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.376"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            106..>621
                     /locus_tag="OO585_004078"
     CDS             106..>621
                     /locus_tag="OO585_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6631463.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECS"
BASE COUNT          159 a          167 c          164 g          131 t
ORIGIN      
        1 cctaaatagc tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat
       61 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca
      121 ccaatttccc gtgacgaacg acgcctgatg cagaaagcca tccataaaac acacgataaa
      181 aattatgccc gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac
      241 gttgccagaa cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg
      301 cagtcgggtg ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt
      361 gagcatatct gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac
      421 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc
      481 cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg cggggattgt gtggcgaagg
      541 gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg aaaagatggc agcaatccat
      601 aaagcactgg acgaatgcag c
//
LOCUS       DAJTYD010000372          621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTYD010000372 DAJTYD010000000
VERSION     DAJTYD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.377"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>621)
                     /locus_tag="OO585_004079"
     CDS             complement(<1..>621)
                     /locus_tag="OO585_004079"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6631464.1"
                     /translation="LMEGISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDT
                     AKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEE
                     AHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKA
                     HPQLAEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT          122 a          183 c          137 g          179 t
ORIGIN      
        1 cgtattttgc gaagccttac gggtagcgat tttcgccgga tttgcctgta actcattgat
       61 atatttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc gtcgggtaaa
      121 ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat tccagctctg
      181 ctgcgctttt tcgccttttt cacgggcatc ccaggcgtga taaatctctt tagggatctc
      241 aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt cttcccccag
      301 tggtgcgccg tgcgcctctt ccttacctgc tttattcggc gaaccaaagc caatcaccgt
      361 acggcagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga tcgcttcctt
      421 caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat aggcttcaaa
      481 acgttttgcc gtatcgtcgg taaaccagcc ttcggtttct ccatcaatcg aaataccgtt
      541 gtgatcgtaa aaaccaatca gcttgcccag tcctagcgtg cccgccagcg aacagacttc
      601 gtgggaaata ccttccatca g
//
LOCUS       DAJTYD010000373          613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTYD010000373 DAJTYD010000000
VERSION     DAJTYD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.378"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>613
                     /locus_tag="OO585_004080"
     CDS             <1..>613
                     /locus_tag="OO585_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6631465.1"
                     /translation="GLVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHT
                     MGPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKSDGSLIKEYSVVALQGLTLVL
                     FIFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETF
                     IPHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGML"
BASE COUNT          138 a          133 c          163 g          179 t
ORIGIN      
        1 ggggctggtg gttgtttttt catgggtgtt tatctttgcg ctctatttca caccttatgc
       61 gctaattgtt ggtcagttag gctcgacctt caaagatggg aagggcgggg tcagtacctg
      121 gattaaacac acgatgggac ccggactggc ttatctcgcc gcgtggacct actgggtggt
      181 gcatattccc tatctggcac aaaaacccca ggcaattctg attgcgctcg gttgggcgat
      241 gaaaagcgac ggttcgctaa tcaaagaata ttcagtcgta gcgttacagg ggttaacgct
      301 ggtgctgttt atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt
      361 cggttctgtg gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt
      421 aaccgcgcct gcgattactg aagtgcatat tgcgaccaca aacattacct gggaaacgtt
      481 cattcctcat atcgacttta cctacattac cactatttca atgctggttt tcgcggttgg
      541 cggagcagag aagatttctc cttacgttaa tcaaacgcgc aacccaggaa aagaatttcc
      601 aaaagggatg tta
//
LOCUS       DAJTYD010000374          606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTYD010000374 DAJTYD010000000
VERSION     DAJTYD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.379"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>606
                     /locus_tag="OO585_004081"
     CDS             <1..>606
                     /locus_tag="OO585_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HCR6631466.1"
                     /translation="LACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATD
                     LWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQE
                     PGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLL
                     KAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKK"
BASE COUNT          140 a          154 c          188 g          124 t
ORIGIN      
        1 tgctggcctg cggcacgcgg atactgggag tgaaggaata catctgcgat aaacctgagt
       61 gcccccacgt cagatacgtc actaactcat gcggcagccg tgcctgcccg tcctgcggaa
      121 aaaaggccac agacctgtgg atagcgacac agctgaatcg tcttcctgac tgcgactggg
      181 tacacctggt cttcaccctg ccggacacgc tgtggccggt gttcgaaagc aaccggtggc
      241 tgctgaatga cgtgtgccgt ctggcggtgg agaatctgct gtatgccgcc cggaaacggg
      301 ggcaggaacc cggtatcttc tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc
      361 cgcatgtaca tgtgtctgta acctgtggag gtctgaataa gcatggtcag tggaaaaagc
      421 tgagcttcct gaaagacgcg atgcgttcac ggtggatgtg gaatatgcgg cagctgcttc
      481 tgaaagcgtg gtcagagggg ctggcgatgc cggagtcgtt gtcacatatc acgacggaat
      541 cacagtggag aagcctggtg ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga
      601 aaaaaa
//
LOCUS       DAJTYD010000375          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTYD010000375 DAJTYD010000000
VERSION     DAJTYD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.380"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..479
                     /locus_tag="OO585_004082"
     CDS             <1..479
                     /locus_tag="OO585_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR6631467.1"
                     /translation="EAGRINGKYGQLGWTPLYYLNQHFDRKLLMKIFRYSDVGLVTPL
                     RDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELTSALIVNPYDRDEVAAALDRALT
                     MSLAERISRHAEMLDVIVKNDINHWQECFISDLKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(486..>605)
                     /locus_tag="OO585_004083"
     CDS             complement(486..>605)
                     /locus_tag="OO585_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="ppGpp-dependent; membrane-associated, stress
                     protein produced under conditions of nutrient deprivation,
                     osmotic shock and oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6631468.1"
                     /translation="DLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT          163 a          140 c          155 g          147 t
ORIGIN      
        1 atgaagctgg acgaattaat ggtaaatacg ggcaattagg ctggacgccg ctttattatt
       61 tgaatcagca ttttgaccgt aaattactga tgaaaatatt ccgctactct gacgtgggct
      121 tagtgacgcc actgcgtgac gggatgaacc tggtagcaaa agagtatgtt gctgctcagg
      181 acccagccaa cccgggcgtt cttgttcttt cgcaatttgc gggagcggca aacgagttaa
      241 cgtcggcgtt aattgttaac ccctacgatc gtgacgaagt tgcagctgcg ctggatcgtg
      301 cattgactat gtcgctggcg gaacgtattt cccgtcatgc agaaatgctg gacgttatcg
      361 tgaaaaacga tattaaccac tggcaggagt gcttcattag cgacctaaag cagatagttc
      421 cgcgaagcgc ggaaagccag cagcgcgata aagttgctac ctttccaaag cttgcgtagg
      481 agcggttaat cacccgtaag tggaactaac agcacatcga cctcacttga ggcaatcact
      541 cttttcgccg agcaggatgc tcgcgaaaag aaactgtgat tgtgattacc gcaaatcacc
      601 aaatc
//
LOCUS       DAJTYD010000376          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTYD010000376 DAJTYD010000000
VERSION     DAJTYD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.381"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            206..>591
                     /gene="livK"
                     /locus_tag="OO585_004084"
     CDS             206..>591
                     /gene="livK"
                     /locus_tag="OO585_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6631469.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPEL"
BASE COUNT          187 a          127 c          132 g          145 t
ORIGIN      
        1 ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga ctgaaagata
       61 gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag acaggcaaaa
      121 acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca aagcaacaca
      181 acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca tcgcagggat
      241 gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg ccgttgtcgg
      301 cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc
      361 gattaaagac attaatgcca aagggggaat taagggcgac aagctggttg gcgtggaata
      421 tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg ttaatgacgg
      481 cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat cagatatcta
      541 cgaagacgaa ggtattctga tgatctcacc gggagcgacc aacccggagc t
//
LOCUS       DAJTYD010000377          573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTYD010000377 DAJTYD010000000
VERSION     DAJTYD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.382"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            68..>573
                     /gene="fliF"
                     /locus_tag="OO585_004085"
     CDS             68..>573
                     /gene="fliF"
                     /locus_tag="OO585_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6631470.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSP"
BASE COUNT          137 a          155 c          154 g          127 t
ORIGIN      
        1 tgacccggga gtgagtcttg ttccactttg ccaataacgc cgtccataat cagccacgag
       61 gtgcgcgatg aatgcgactg cagcccagac aaaatctctt gagtggctta atcgcctgcg
      121 tgcgaatccg aaaattccat tgattgttgc cggttccgcg gcagtggcgg tcatggtcgc
      181 actgatcctg tgggcgaaag cccccgacta ccgcacatta ttcagcaatc tttccgatca
      241 ggatggtggc gcaattgtca gccaactgac gcaaatgaat attccttacc gcttcagcga
      301 agccagcggc gctattgaag ttccggcaga taaagttcac gagctgcgtc tgcgcctggc
      361 gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa ctactagatc aggaaaagtt
      421 tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg gcgctggaag gcgagctttc
      481 tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc gtacatctgg caatgccgaa
      541 accgtcttta ttcgtccgtg aacaaaaatc ccc
//
LOCUS       DAJTYD010000378          572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTYD010000378 DAJTYD010000000
VERSION     DAJTYD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.383"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..171
                     /locus_tag="OO585_004086"
     CDS             <1..171
                     /locus_tag="OO585_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6631471.1"
                     /translation="DRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAWQ
                     HPKRTERPGIRI"
     gene            191..>572
                     /locus_tag="OO585_004087"
     CDS             191..>572
                     /locus_tag="OO585_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6631472.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAE"
BASE COUNT          164 a          151 c          156 g          101 t
ORIGIN      
        1 gatcgtgacg ggttatgcct gttcgccaaa cgcctggaac ggggccgctt cgtctggccg
       61 gtgacccggg aagggaaagt gcacctgacg ccagctcagt tatccatgct actggagggg
      121 atcgcgtggc aacatcccaa acggacagaa cggcctggca tccggatata acccgtgata
      181 aaacaaggga atgaacaatg aactccccga tgatattgag ctgcttaaag ccatgttgcg
      241 taagcaacag agtcggcttc gacagtatgc ctgtcaggtc gcgggctatg agcaggaaat
      301 tgaacggctg aaagcgcaac tcgacaggtt gcgtcgtatg ctgttcggcc agagttcaga
      361 gaaaaagcgt cataagcttg aaaatcagat ccgacaggca gaaaaacgac tgtcggaact
      421 ggaaaaccgg ctgaacacag ccagaaatct tctggaagat gcatcgtcag tcacagattc
      481 acctgacacc agtcccccgt cagaaaaccc gatcgccagt aagcctgaat ccccgggacg
      541 aaaatcttca cgaaaaccgc tgccggcaga ac
//
LOCUS       DAJTYD010000379          568 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTYD010000379 DAJTYD010000000
VERSION     DAJTYD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.384"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>568
                     /locus_tag="OO585_004088"
     CDS             <1..>568
                     /locus_tag="OO585_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6631473.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDE
                     NPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     VINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDL
                     AYLIKFDGNYKDGMTVWLFSCNTSKGQNS"
BASE COUNT          155 a          129 c          143 g          141 t
ORIGIN      
        1 tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc atcgcggcct gccgctggcg
       61 ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat atgatgaatg gagcaacctg
      121 ctgagtgatg agaacccgca tcatctgcag cagctcattc gcctgccggg tcagcagtat
      181 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcgg
      241 tatatcactc aggatccgat tgggctgaag gggggatgga atttttatca gtatccgttg
      301 aatccggtca taaatgtaga tccgcaaggt ttggttgata taaatttata ccccgaaagt
      361 gatcttatcc attctgtagc tgatgagatt aatatcccag gcgttttcac aatcgggggg
      421 catggtaccc ccacatctat tgaatccgca acgcgcagta tcatgacagc taaagatcta
      481 gcatatctaa ttaaatttga tgggaattat aaagatggga tgacagtttg gttattttct
      541 tgtaatacaa gtaaaggaca aaattcat
//
LOCUS       DAJTYD010000380          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTYD010000380 DAJTYD010000000
VERSION     DAJTYD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.385"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            325..>566
                     /locus_tag="OO585_004089"
     CDS             325..>566
                     /locus_tag="OO585_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631474.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGT"
BASE COUNT          217 a           93 c          104 g          152 t
ORIGIN      
        1 gactctatca tcgccaatca tcagacattg aaatagacga cactgagatg acgcttacaa
       61 ttcttttggt ttctgtaata agtaatctga catcccctgt gcccagaaga ggagagggat
      121 atcaagccca agggttaata attgatacta cggttaaaat tgccttcagt aaaggaaacg
      181 tgattaaatt caggggattt cagagtgaaa accatacaac atattaaaat ctgttcattt
      241 agctataata catacaagaa agtgtccagt tagataatgt tatctaaata accacagata
      301 aaaacgcaca taattggggg ggggatgaaa atacctgaag cagtaaatca tattaatgtt
      361 cagaataata ttgatcttgt tgatggaaaa acgaatccga ataaagcaac aaaagcatta
      421 caaaaaaata tattgcgcgt aacaaactca tcttcttctg gcataagtga aaaacattta
      481 gatcactgtg ctaatactgt gaaaaacttt ttaaggaagt ctatagcagc gcaaagttat
      541 agcaaaatgt tttctcaagg gaccag
//
LOCUS       DAJTYD010000381          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTYD010000381 DAJTYD010000000
VERSION     DAJTYD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.386"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>566
                     /locus_tag="OO585_004090"
     CDS             <1..>566
                     /locus_tag="OO585_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HCR6631475.1"
                     /translation="SNSTGGVKSVALATGAYAHIRRQFKISIGKMEGIEEPLARIAGN
                     AYVMDAAASLITYGIMLGEKPAVLLAIVKYHCTHRGQQSIIDAMDITGGKGIMLGQSN
                     FLARAYQGAPIAITVEGANILTRSMMIFGQGAIRCHPYVLEEMEAAKNNDVNAFDKLL
                     FKHIGHVGSNKVRSFWLGLTRGLTSSTP"
BASE COUNT          135 a          145 c          162 g          124 t
ORIGIN      
        1 ttccaactca accggcggcg tgaaatcggt agcgctggca acgggcgcgt atgctcacat
       61 tcgccgtcag ttcaaaatct ctatcggaaa gatggaaggg attgaagagc cgctggcgcg
      121 tattgccggt aatgcctacg tgatggatgc tgcggcatcg ctgattacct acggcattat
      181 gctcggcgaa aaacctgccg tgctgttggc tatcgttaag tatcactgta cccaccgcgg
      241 gcagcagtcg attattgatg cgatggatat cactggtggc aaaggcatta tgctcgggca
      301 aagcaacttc ctggcgcgtg cttaccaggg cgcaccgatt gccatcaccg ttgaaggggc
      361 taacattctg acccgcagca tgatgatctt cggacaagga gcgattcgtt gccatccgta
      421 cgtgctggaa gagatggaag cggcgaagaa caatgacgtc aacgcgttcg ataaactgtt
      481 gttcaaacat atcggtcacg tcggtagcaa caaagttcgc agcttctggc tgggcctgac
      541 gcgcggttta accagcagca cgccaa
//
LOCUS       DAJTYD010000382          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTYD010000382 DAJTYD010000000
VERSION     DAJTYD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.387"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..386)
                     /locus_tag="OO585_004091"
     CDS             complement(<1..386)
                     /locus_tag="OO585_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="molybdenum cofactor-binding protein; participates
                     in purine salvage; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6631476.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAA"
BASE COUNT          150 a          136 c          143 g          136 t
ORIGIN      
        1 tgcgccgctt tttctgccgt gagttcatcg cgggccacga cgatggcaat ggcgtcacca
       61 tgatgacgaa catggcgagt tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca
      121 agtgtccagg catgcccagc tgtagcgaat ggaatatcag gcacatcttc ccaggtaaaa
      181 atcgccagta cgcccggcaa acttctggct tgttcatcat taatacttac ggcataacca
      241 tgtgcgatag ggctacgtac atatttcgca taacacatgc ccgccataac ataatcgtca
      301 gtatatcgtg cccgcccggt gaccttagca atggcatcga cgcgcatgca tgattcaccc
      361 gtagcggttg cttcccgcgc ttccatagaa atcccctcga tttgaacatt tggttcttat
      421 ggcaaagatt tagtctaagc aggcaagatt tacgccagaa acgacgataa gcgttagggg
      481 aggtaggaag tgtgatgacg ttaaaaaaac acagatatga tacgaaacag gagtctgaat
      541 aggggcagtg atacgtatca atatg
//
LOCUS       DAJTYD010000383          552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTYD010000383 DAJTYD010000000
VERSION     DAJTYD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.388"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>552
                     /locus_tag="OO585_004092"
     CDS             <1..>552
                     /locus_tag="OO585_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631477.1"
                     /translation="IRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQ
                     PEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIR
                     AKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDE
                     WENLHALKEKRLNGMPKTTPKSGQ"
BASE COUNT          194 a          107 c          111 g          140 t
ORIGIN      
        1 atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca
       61 gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt
      121 catgcttatc aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat
      181 tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca
      241 tttaacttta tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat
      301 atacgagcga agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag
      361 gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa
      421 gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa
      481 tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc
      541 aaaagtggcc ag
//
LOCUS       DAJTYD010000384          537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTYD010000384 DAJTYD010000000
VERSION     DAJTYD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.389"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>537
                     /locus_tag="OO585_004093"
     CDS             <1..>537
                     /locus_tag="OO585_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6631478.1"
                     /translation="KKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADL
                     YSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHL
                     KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPR
                     VVKERPWKYPTAPKKSQS"
BASE COUNT          157 a          111 c          169 g          100 t
ORIGIN      
        1 gaaaaaagtg gccgggactg ggaaatgaag tgacagcccg cctgctgacc gtgacgcgca
       61 aaggaaaagt ctgccatctg ctgacgtcga tgacggacgc catgcgcttc cccggaggag
      121 aaatggcgga tctgtacagt catcgctggg aaattgaact gggatacagg gagataaaac
      181 agacgatgca actgagcagg ctgacgctga gaagtaaaaa gccggagctt gtggagcaag
      241 agctgtgggg tgtcttactg gcttataatc tggtgagata tcagatgatt aaaatggcgg
      301 aacatctgaa aggttactgg ccgaatcaac tgagtttctc agaatcatgc ggaatggtga
      361 tgagaatgct gatgacattg cagggcgctt caccgggacg tataccggag ctgatgcgcg
      421 atcttgcaag tatgggacaa cttgtgaaat taccgacgag aaggggaagg gccttcccga
      481 gagtggtaaa ggagaggccc tggaaatacc ccacagcccc gaaaaagagc cagtcag
//
LOCUS       DAJTYD010000385          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTYD010000385 DAJTYD010000000
VERSION     DAJTYD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.390"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(39..218)
                     /locus_tag="OO585_004094"
     CDS             complement(39..218)
                     /locus_tag="OO585_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6631479.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            292..>534
                     /gene="pabB"
                     /locus_tag="OO585_004095"
     CDS             292..>534
                     /gene="pabB"
                     /locus_tag="OO585_004095"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416326.1"
                     /note="para-aminobenzoate synthase component 1; catalyzes
                     the formation of 4-amino-4-deoxychorismate from chorismate
                     and L-glutamine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HCR6631480.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQ"
BASE COUNT          116 a          149 c          123 g          146 t
ORIGIN      
        1 catcggcttc tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa
       61 cgcgccacga ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt
      121 gcctggccgc tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc
      181 tgctgttcat gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt
      241 ggcgctaatt atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg
      301 ttatctcccg ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc
      361 cgcttaagcc acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat
      421 agccgctttg atattgtggt cgccgagccg atttgcactt taaccacttt cggtaaagaa
      481 accgttgtta gtgaaagcga aaaacgcaca acgaccactg atgacccgct acag
//
LOCUS       DAJTYD010000386          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTYD010000386 DAJTYD010000000
VERSION     DAJTYD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.391"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(3..>525)
                     /locus_tag="OO585_004096"
     CDS             complement(3..>525)
                     /locus_tag="OO585_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR6631481.1"
                     /translation="TKEKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIA
                     LPCATQNELDVGAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGG
                     VATSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGF
                     VKVADAMLAQGVI"
BASE COUNT          108 a          159 c          132 g          126 t
ORIGIN      
        1 acttaaatca caccctgcgc cagcatcgca tcggcaacct tcacaaaacc ggcaatgttc
       61 gcgccctgca cgtagttggt ttgctcacct tcaccaccat gctcaacaca ggcatggtgg
      121 atatccagca tgatgtgatg caaacgtgcg tcaactttct cggctttcca gcccaggcgc
      181 gcagcgtttt gcgccatttc aaggcctgac gttgctacac cgccagcatt agccgcttta
      241 cccggtgcaa acagtacgcc tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc
      301 atatttgccc cttcggcgac ggctttaacg ccattggcga taagctgatg cgcggcgcca
      361 acatccagtt cgttctgggt ggcgcaaggc agggcgatat caaccggtac agaccacggc
      421 tgttggcctt cgagatagac cagaccaaat tctttggcgt aatctgccac tcgaccatcg
      481 cggctggctt tgatttcgat aagacgtgcc agtttctctt tcgtg
//
LOCUS       DAJTYD010000387          523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTYD010000387 DAJTYD010000000
VERSION     DAJTYD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.392"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            1..523
                     /gene="ycaL"
                     /locus_tag="OO585_004097"
                     /pseudo
     CDS             1..523
                     /gene="ycaL"
                     /locus_tag="OO585_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT          145 a          124 c          135 g          119 t
ORIGIN      
        1 gaagaatact aaattactgc tggcgattgc gacctctgca gcattactga cagggtgtca
       61 aaatacccac ggtattgata ccaatatggc tatcagctcc ggtttaaatg cctataaagc
      121 agcaacatta agcgatgccg atgcaaaagc gattgccaat cagggctgtg ccgaaatgga
      181 cagcggcaat caagtcgcaa gtaaatccag caagtacggt aaacgtctgg caaaaatcgc
      241 caaagcattg ggtaacaata ttaatggcac gccggtcaac tataggttta tatgaccagc
      301 gacgtcaacg catgggcgat ggcgaacggc tgtgtccgtg tctacagtgg cctgatggac
      361 atgatgaatg acaacgaaat tgaaggtgtt ctgggccatg aactgggcca cgtcgcgttg
      421 ggtcactcgc tggctgaaat gaaagtttct tatgcgatcg ttgccgcacg cgatgccatt
      481 tcagctacca gcggtgtggc ttcccagctt tcccgctcac aat
//
LOCUS       DAJTYD010000388          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTYD010000388 DAJTYD010000000
VERSION     DAJTYD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.393"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..279
                     /locus_tag="OO585_004098"
     CDS             <1..279
                     /locus_tag="OO585_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6631482.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          125 a          115 c          139 g          135 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcatt gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca
      481 caatccgggc tgagttccct ctaagtgatc tagg
//
LOCUS       DAJTYD010000389          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTYD010000389 DAJTYD010000000
VERSION     DAJTYD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.394"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(27..356)
                     /locus_tag="OO585_004099"
                     /pseudo
     CDS             complement(27..356)
                     /locus_tag="OO585_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          138 a          118 c          103 g          149 t
ORIGIN      
        1 accagcggga gcatttgaac agaaacttac ggttttgctc tttcggtagg ctcagtaccg
       61 taaggttctc catcaacaaa ataagtacca ttaggtaata cgtgtttata gggcagtaat
      121 gatatatggg ctaatacgtc atcatcaata acctcaccat tttcacgtaa cccttcaaca
      181 atattgctta ttcttgatgt attccagtac aggattgcat tcgataccag actcagggcg
      241 ctggccttat tcatgatttc ttcgtaatca ccggtcgtga attcaccctg atttgcaaaa
      301 aagatccatc ggggtaattt atgtcgatat tcgcctttat tgagctgaag ctcgagtagg
      361 cagcctgacg gctgcggctt gtcatggtct gcaattaccg ttataaaaac aggcaatgtc
      421 attgtctttc aggtggttat atggcccgtt cagctaaacc ccgtaaacgt aaacccgcct
      481 ctcaaagaag caaacttccc cgctatgt
//
LOCUS       DAJTYD010000390          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTYD010000390 DAJTYD010000000
VERSION     DAJTYD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.395"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..472)
                     /locus_tag="OO585_004100"
     CDS             complement(<1..472)
                     /locus_tag="OO585_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631483.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT          143 a          122 c          119 g          124 t
ORIGIN      
        1 agtcgttaat gacttttaca ccgcatgggt tcatcggcgc ttcaccaatc cccataccga
       61 tacgcaccaa cacgaactgc gtaaagttgt gcaccatgcc agacattgcc tggaacagtg
      121 accagaagaa catccccagt cccagcatca ggcgtgggcc tttacgatcc aatagtgggc
      181 cgcaaggaag ttgcgcaatc ccgtaagcga gtgaaaacac ggagagcaaa gcgccgattt
      241 cagtggcact taatcccaat tcttcacgaa tcgttaaatt tgctaccgac agcgaactgc
      301 ggtcgagata attgattacc gccgcaaaaa ataataacaa catggcggtg gtttgaatgc
      361 gtttaattcg ggtactgcgt tgaactaatc catctggtgg cacgggaata tctgccgacg
      421 aagatggagt aaatgaagaa cgcggatcga tggtgatatt ttctttttcc acgccggact
      481 cctgtattta taattttaat tgtttacg
//
LOCUS       DAJTYD010000391          506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTYD010000391 DAJTYD010000000
VERSION     DAJTYD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.396"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(68..506)
                     /gene="tnpA"
                     /locus_tag="OO585_004101"
                     /pseudo
     CDS             complement(68..506)
                     /gene="tnpA"
                     /locus_tag="OO585_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          115 a          149 c           92 g          150 t
ORIGIN      
        1 ctataaggct ctcttaccag ccgcgcccta acaggcgcac acgatctgac atttgcatcc
       61 aacttcgtta cttacggtcc gtaaacgggc tgcccggaca gggaatcgat aactgctctc
      121 ccattttatc ctcttcaagc tggtgcttta tgtaatcctg tatcttcgcc gtgttcttac
      181 ccactgtatc gacgtagtcc cccctgcacc agaactccct gttcctgtat ttgaatttca
      241 aatcaccaaa ctgctcgtaa agcatcagac tgcttttccc tttcagatat cccataaagc
      301 cggatacgct cattttgggc gggatctcca caagcatatg gatatgatct gcacagcatt
      361 cagcttccag aatccgtacg cttttccact cacacagctt tctcaaaata ctgcctgttg
      421 ctctacgctt ctctctgtag aacacctgtc ttcggtattt tggcgcaaaa aactatgtga
      481 tatttacagt tccatcgggt gtgcgc
//
LOCUS       DAJTYD010000392          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTYD010000392 DAJTYD010000000
VERSION     DAJTYD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.397"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>501
                     /locus_tag="OO585_004102"
     CDS             <1..>501
                     /locus_tag="OO585_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6631484.1"
                     /translation="ETAEQWQYDKRGWLTDISHLSEGHRVTVHYRYDEKGRLTGERQT
                     VHHPQPEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLVD
                     FTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPR
                     QTRSYSY"
BASE COUNT          117 a          161 c          151 g           72 t
ORIGIN      
        1 gaaaccgcag agcagtggca gtatgacaaa cgtggctggc tgacagacat cagccatctc
       61 agcgaagggc accgggtgac ggtgcattac aggtatgatg agaaaggccg gctgaccggt
      121 gagcgccaga cggtgcatca cccgcagccg gaagcactgc tctggcagca tgagaccaga
      181 catgcgtaca acgcgcaggg actggcgaac cgctgtatac cggacagcct gcccgccgtg
      241 gaatggctga cctacggcag cggctggctg gcaggtatga agctcggcga cacaccgctg
      301 gtggatttca cccgcgaccg cctgcaccgg gaaacgctgc gccgcttcgg ccgttatgaa
      361 ctctccaccg cttacacccc tgccgggcag ttacagagcc agcacctgaa cagcctgcag
      421 tatgaccgcg attacacctg gaacgacaac ggcgaactca tccgcatcag cagcccgcgc
      481 cagacccgga gttacagcta c
//
LOCUS       DAJTYD010000393          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTYD010000393 DAJTYD010000000
VERSION     DAJTYD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.398"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            170..>493
                     /locus_tag="OO585_004103"
     CDS             170..>493
                     /locus_tag="OO585_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631485.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENG"
BASE COUNT          135 a          122 c          116 g          120 t
ORIGIN      
        1 gtgtccaagg cgaatgtcct cttagaatga tcgtgcgcgc atgttacgcc agcaaaattt
       61 tgccgtcaac acgcaacaga acaccacaga acaatagttg aacttcacgc tttttggtac
      121 tcctcaaggt cgctaacagg ataaaaaaat cccacatcca ggacctataa tggcgcatcg
      181 gctactcatt ggtaaaggaa tgatcacctt gaatctcaaa cggatttttc tcaccctcac
      241 cttacttccc ttgttttctg ttgccgctga tgattgcgca ctctcagatc cgacgctgac
      301 cgtacaggcg tataccgtta atcctcaaac agagcgggtg aaaatgtact ggcaaaaagc
      361 caatggcgaa gcgtggggaa cgttacatgc tctgctggcg gatattaata gtcagggtca
      421 ggtgcagatg gcgatgaacg gcggcatcta tgatgaaagc tatgcgccgc tcggtttgta
      481 catcgaaaac ggt
//
LOCUS       DAJTYD010000394          491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTYD010000394 DAJTYD010000000
VERSION     DAJTYD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.399"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..124)
                     /locus_tag="OO585_004104"
     CDS             complement(<1..124)
                     /locus_tag="OO585_004104"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6631486.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLT"
     gene            complement(318..>491)
                     /locus_tag="OO585_004105"
     CDS             complement(318..>491)
                     /locus_tag="OO585_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6631487.1"
                     /translation="PVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETV
                     SPFPGGKHGLAKV"
BASE COUNT          143 a          108 c          119 g          121 t
ORIGIN      
        1 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
       61 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
      121 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
      181 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
      241 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taatctaaat
      301 agctgcgctt aataccgcta cacttttgcc agcccatgtt tgcctccggg gaatgggctg
      361 acggtttcca taaaatggcg aacttttttc aacagttgcc acattgagcg gcactgatga
      421 ttacgcgtta ttgtgtcgtg aagtgcctgc catagccgtt caacatgatt cacccatggc
      481 gagtaaaccg g
//
LOCUS       DAJTYD010000395          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTYD010000395 DAJTYD010000000
VERSION     DAJTYD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.400"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>489)
                     /locus_tag="OO585_004106"
     CDS             complement(<1..>489)
                     /locus_tag="OO585_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6631488.1"
                     /translation="ASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFARILSPATE
                     PSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALINGIIGGI
                     GGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVAYLSFSPY
                     LQ"
BASE COUNT          133 a          147 c          112 g           97 t
ORIGIN      
        1 tttgcaggta tggggagaaa ctcaggtaag cgacgaattc gttaatcgcc agtttctgcc
       61 caatcaggct acccgcaaga ttggcatcac tccagtccac acccatgatc cacgccagcg
      121 gtgccagcac atagccaaaa atactttcca gagaggcatt ggcgaaacca aaccagccac
      181 caattccgcc gataatgccg ttgatcagcg caataattgc gacaaatgcc attaccaccg
      241 tcgctacacc agcggcgatt ttcagcccgg tcatcgcacc gctcgccgcc gcttcgataa
      301 agctttttgg cggcgtttcg ctgaacgaca gattttcaaa tgtgacctgc gaaggctcgg
      361 tggccgggct aagaatacgt gcaaacaaaa taccacctgg gatcgccatc agcgatgccg
      421 ctaacaggta gtcaattggt acgcccattc cggcataacc aatcatcatc gaaccagcaa
      481 tggacgcca
//
LOCUS       DAJTYD010000396          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTYD010000396 DAJTYD010000000
VERSION     DAJTYD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.401"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            197..>487
                     /gene="panM"
                     /locus_tag="OO585_004107"
     CDS             197..>487
                     /gene="panM"
                     /locus_tag="OO585_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6631489.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWM"
BASE COUNT          126 a          112 c          116 g          133 t
ORIGIN      
        1 taacggcaca aagggcggaa taattcacta ccattcaggg gattatgctg ggtatttttc
       61 attctctaat gttttaactt tgtaattatt gctgttaaaa aattaatcac ctgccaaaag
      121 aaataaaaaa gagaaagcct ccgattaaat tatttcgcta cactggttcc acttttgtga
      181 tttacacggg ttacccatga agctgaccat cattcgatta gaaaacttta gcgaccagga
      241 ccggattgac ctgcaaaaga tctggccgga gtattcccct tcctctttac aggttgacga
      301 taaccaccgt atctacgccg cgcgttttaa cgagcgcctg ctcgctgccg tacgggtaac
      361 cttaagcggc accgagggag cactggattc cctgcgtgtg cgggaagtca cccgacgtcg
      421 cggtgtgggg caatatctgc tggaagaggt tttgcgtaac aatcctggcg tttcatgctg
      481 gtggatg
//
LOCUS       DAJTYD010000397          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTYD010000397 DAJTYD010000000
VERSION     DAJTYD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.402"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            272..>480
                     /locus_tag="OO585_004108"
     CDS             272..>480
                     /locus_tag="OO585_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6631490.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNS"
BASE COUNT          151 a          110 c           86 g          133 t
ORIGIN      
        1 tgccccttta tcttatttct gatacttagc agcaaataaa taacgcgata aaaaaagcca
       61 aacgttttcg tattttacaa acaaccagaa gctggcatca atttgtgatc aaccccacac
      121 attatccgtc aaattagtct tttgcagccg cgcggataat tctggcacac ttattgttag
      181 tcccaggtat agctgtgaaa acaccaatca ctttggcaag tcacagtgaa ataaaccact
      241 ttgcctgtca ttccactacc gggactttat gatgaaaact gttaatgagc tgattaagga
      301 tatcaattcg ctgacctctc accttcacga gaaagatttt ttgttaacgt gggaacagac
      361 gccagatgaa ctgaaacaag tactggacgt tgccgcagca ttaaaagcac tgcgtgctga
      421 aaacatctca accaaagtct ttaatagtgg attaggtatt tccgtattcc gcgacaactc
//
LOCUS       DAJTYD010000398          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTYD010000398 DAJTYD010000000
VERSION     DAJTYD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.403"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            108..455
                     /gene="grxD"
                     /locus_tag="OO585_004109"
     CDS             108..455
                     /gene="grxD"
                     /locus_tag="OO585_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6631491.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
BASE COUNT          134 a          115 c          124 g          103 t
ORIGIN      
        1 aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg
       61 ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta
      121 tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac
      181 cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg
      241 aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat
      301 atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg
      361 atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg
      421 ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacgg
//
LOCUS       DAJTYD010000399          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTYD010000399 DAJTYD010000000
VERSION     DAJTYD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.404"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>472)
                     /gene="glnP"
                     /locus_tag="OO585_004110"
     CDS             complement(<1..>472)
                     /gene="glnP"
                     /locus_tag="OO585_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6631492.1"
                     /translation="FARTFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDL
                     RIDPFTAAVVTIMINSGAYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLA
                     LRRMLPPLGNQWIISIKDTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFY"
BASE COUNT          128 a          129 c          127 g           88 t
ORIGIN      
        1 atagaacacc gccacggcgc tccagatctc aagggcgcgg aagttaccgg caataatttc
       61 ttgcccctga cgggtcagtt ccgccacgcc gataacaata aacagcgagg tgtctttaat
      121 gctgatgatc cactggttac ccagcggcgg cagcatacga cgcagtgcca gcggtaaaat
      181 gacgtagcga atggtttccc aacgtgaaag accgagcgcc agtcctgctt cacgaaaacc
      241 tttgtgaata gacagcaccg caccacgcgt gatttccgca atgtacgcgc cggagttgat
      301 catgatggtg accaccgccg cagtaaatgg gtcgatgcgt aagtcgttaa acgccatcgg
      361 cagggcgaaa tagataaaca tcacctggac gacgataggt gtgccgcgga tcacttcaat
      421 aaagaccagc gcgacgtggt tggctatcca accaccgaag gtgcgtgcaa aa
//
LOCUS       DAJTYD010000400          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTYD010000400 DAJTYD010000000
VERSION     DAJTYD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.405"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..311)
                     /locus_tag="OO585_004111"
     CDS             complement(<1..311)
                     /locus_tag="OO585_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631493.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     L"
BASE COUNT          138 a          112 c          100 g          122 t
ORIGIN      
        1 ctgagatctg gcgtgctata ggccagtaca cagacgatcc cgaatggcag tgcgccaaac
       61 agtacccacg gacgaaattt accccagcga gagcgcgttc gatcggccaa caaccccatg
      121 caaggatcgg aaatcgcatc cagtgcacga gcgaccaaaa acatggttcc gacaaaaccg
      181 gcaggaatgc caaaaatatc ggtataaaag aacatcatat ataacattac gttatcgaaa
      241 ataatgtggc tggcggcgtc tcccatgcca taaccaattt tctctttaac ggacaacact
      301 tcactcttca tctgcttttc cttgatgcta taggggtgct gagaccgata acgcattttg
      361 taaaccatcc ctgtgagagc gaatattccg atatctggtt attaatttat gattcttgtt
      421 ttatgtgatc gtggtagcgt taattccgct catatgtcat tgtaaaatat gg
//
LOCUS       DAJTYD010000401          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTYD010000401 DAJTYD010000000
VERSION     DAJTYD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.406"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(24..>471)
                     /locus_tag="OO585_004112"
     CDS             complement(24..>471)
                     /locus_tag="OO585_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6631494.1"
                     /translation="SDYLKLQALFARVAEEKHRRGELEKLHHQLVDTYTSLNRQYAEL
                     LSEYKHLRRYFGVTVQVPYTDVWTHKPVQFYPGKHPCEKPAEMLQQIISASSRPGDLI
                     ADFFMGSGSTVKAALALGRRAIGVELETERFEQTVREVQDLVSQNG"
BASE COUNT          105 a          148 c          100 g          118 t
ORIGIN      
        1 gacgcgtaat taattcttca atatcatccg ttctggctga ctaaatcctg tacttcccga
       61 accgtctgct caaaacgttc agtctccagc tcaacgccaa ttgcacgacg cccgagcgcc
      121 agtgccgctt tcactgtcga acccgacccc atgaaaaaat ctgcaatcag gtcacccgga
      181 cgactgctag cgctgattat ctgctgcagc atttctgccg gtttttcgca cggatgtttc
      241 ccgggataga actgcaccgg tttatgcgtc cacacatcgg tatacggcac ctgcaccgtc
      301 acgccaaaat accgccgcag atgtttatat tcactcagca gctccgcata ctgccggttc
      361 agtgaggtat acgtatccac cagctggtgg tggagctttt ccagttcacc acgccgatgc
      421 ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct t
//
LOCUS       DAJTYD010000402          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTYD010000402 DAJTYD010000000
VERSION     DAJTYD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.407"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..262)
                     /locus_tag="OO585_004113"
     CDS             complement(<1..262)
                     /locus_tag="OO585_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCR6631495.1"
                     /translation="MTEADLYPHLAHLAGGQVSPYVAPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLA"
     gene            complement(259..>461)
                     /locus_tag="OO585_004114"
     CDS             complement(259..>461)
                     /locus_tag="OO585_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631496.1"
                     /translation="PDTGNSDNTMKADNPRNAFYWRFVEMGTVNMPPHPFVRPAFDVR
                     SEQAAQVAIARMNRAIDEVLRR"
BASE COUNT           98 a          160 c          120 g           83 t
ORIGIN      
        1 gggccagcag cattatggct tcacgggcgt cctgacgtat ctgacgcgcc tgcgtcacag
       61 tcccggcata aacgtctatc tgcaccgaca ctgaggactc cgcctgcccg cccatcacgt
      121 ccgctgacac cgatgaaatc aggctgaaaa ccacccacgg aagcgccacc gacggcctgc
      181 catccagcag gggggccaca tacggggaca cctgcccgcc ggcaagatgc gccagatgag
      241 gatacaaatc cgcctccgtc atcgtctcag tacctcatca atggcccggt tcatccgcgc
      301 aatcgccacc tgagctgcct gttcactgcg cacatcaaac gccgggcgca caaacgggtg
      361 cggtggcata ttcacggtcc ccatttccac aaaccgccag tagaaagcat tgcgcgggtt
      421 atccgccttc atggtgttat cgctgttacc ggtgtccgga t
//
LOCUS       DAJTYD010000403          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTYD010000403 DAJTYD010000000
VERSION     DAJTYD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.408"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            16..237
                     /locus_tag="OO585_004115"
     CDS             16..237
                     /locus_tag="OO585_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6631497.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            270..>443
                     /locus_tag="OO585_004116"
     CDS             270..>443
                     /locus_tag="OO585_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCR6631498.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
BASE COUNT          132 a          108 c           92 g          111 t
ORIGIN      
        1 gaggaatgtt gcatcatgag acctaacatt tcaatcactc ttaccacgcc tcatgtgact
       61 attgaacgct atagcgagct gacagggcta tccatcgata ccatcaatga catgttggct
      121 gatggacgcc ttatccgtca ccgtctgcgc aaagataaaa aacgcgaaaa agtgatgatc
      181 aacatagcag caatgaccgt tgatgcgctt tcagaatgca atctaaacct taattagttc
      241 gattctgaaa tacatcagag gcattgacca tgtttgatta ccaagtttcc aaacatccac
      301 attttgatga agcctgtcgt gcatttgcac tgcgccacaa tctggtgcaa ctggcagaac
      361 gtgcaggcat gaatgtgcag attctgcgga acaagctgaa cccagctcag cctcatttat
      421 taaccgcacc agaaatctgg ctg
//
LOCUS       DAJTYD010000404          441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTYD010000404 DAJTYD010000000
VERSION     DAJTYD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.409"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>441)
                     /locus_tag="OO585_004117"
     CDS             complement(<1..>441)
                     /locus_tag="OO585_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCR6631499.1"
                     /translation="NEAGMGSTPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTAS
                     AMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAE
                     NNLFFLRLNTPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIMA"
BASE COUNT          112 a          119 c          119 g           91 t
ORIGIN      
        1 gccattatga tatctgccag ttgccacatc agcggaaggc ttagcaaggt gccgccgatg
       61 accgttgcga aggtgcagat ccgcaaacac cagatcgctt taggggtgtt caggcgtaaa
      121 aagaagagat tgttttcggc ataaatgtag ttggcaacga tggagctgaa ggcaaacaga
      181 ataaccacga gggtaacaaa ctcagcaccc caggaaccca ttagcacccg catcgccttc
      241 tggataagct gaataccttc cagcggcatg taggttgtgt cgttacccgc cagtaatatc
      301 agcatggcgc ttgccgtaca gatgaccagg gtgtcgataa aaatgccaat catctggaca
      361 atcccttgcg ctgccggatg cggaggccag gacgccgctg ccgctgccgc gtttggcgtc
      421 gaacccattc ccgcctcatt g
//
LOCUS       DAJTYD010000405          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTYD010000405 DAJTYD010000000
VERSION     DAJTYD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.410"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>438)
                     /gene="rpnB"
                     /locus_tag="OO585_004118"
     CDS             complement(<1..>438)
                     /gene="rpnB"
                     /locus_tag="OO585_004118"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6631500.1"
                     /translation="AGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSS
                     AFPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLK
                     ALFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEG"
BASE COUNT          119 a          109 c           91 g          119 t
ORIGIN      
        1 gccttcttca cgtaatctgt cagcaatggt catcagtttc tccttttctt gtggtgcgcg
       61 ttccgctatc tcaccaataa atgcacgaaa acgctgggca tcccctgttt gtaatacgta
      121 attaaacagg gcttttagct gtctgtcatt agtgttccct gtaactagca gcgaaacaat
      181 ttggtcgact aatcccaaca gatcgcgctg acgaatatgt ttctgaatta actccaacag
      241 cgccattttg cggtgttgca taatctcgtc atccggcacc acggtaatat ccaccaacgg
      301 aaacgctgat gaatatattt tgcgggctat agcaggctcg gcaaattcat caagccagca
      361 gagtgaataa ggataaggac ttctgcaacc atgataaaac agcatcggga tcaccaatgg
      421 aagctcttta tagcccgc
//
LOCUS       DAJTYD010000406          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTYD010000406 DAJTYD010000000
VERSION     DAJTYD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.411"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..408)
                     /gene="eamB"
                     /locus_tag="OO585_004119"
     CDS             complement(<1..408)
                     /gene="eamB"
                     /locus_tag="OO585_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6631501.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQT"
BASE COUNT          120 a          112 c          104 g          102 t
ORIGIN      
        1 tgtttgcggc agaacaaacg tcgacagtgc cgtaacaccg tacaaaatga ttttgacgtt
       61 cacaaactgc aaagcaaagc tggcccaaaa gctgattggt tttgcctgaa gtccgtcttc
      121 ctttgttggg ctggtggcga ttttccacgc cagccagaca atatatgccg cccccgccca
      181 actcaaaagg tgtaccgctg ccgggtcaat cactgccagt gaaaatgaaa tgcccgcaca
      241 cagtaacatc acaatcaaaa atcccagact catccctgcc agcacgcggg tactttgacg
      301 aaatccatgc gacgtagcag agctaagggc gagaatattg ttcggtcttg gcgtcatagc
      361 ggtaatcagg gtgtaagtcc aaaaagcact taaaagggtc ggtgtcactt gttatctcct
      421 ctgttttggt agacagta
//
LOCUS       DAJTYD010000407          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTYD010000407 DAJTYD010000000
VERSION     DAJTYD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.412"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            320..>434
                     /locus_tag="OO585_004120"
     CDS             320..>434
                     /locus_tag="OO585_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6631502.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEK"
BASE COUNT          145 a           90 c          107 g           92 t
ORIGIN      
        1 caaaatgtat tggcgagtaa gaaccgcatt tagtactttc cgggcaaccg ccagacgatt
       61 ctttattggt aatgagaata attaacaatt aacaattaaa gagcgtcgcg aaagaataat
      121 gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg aatcaccaga
      181 ataaataaag tcggtgatag caatccgtaa cgataaagta acctgacagc agaaagtctc
      241 cgagcctgtg cagggtccca atccgggatt acacatgctg gttaatacca gtaattataa
      301 tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag cagctggcgt
      361 tctggctgcg ctaatggcag ggaatgtcgc gctggcagct gatgtacccg caggcgtcac
      421 actggcggaa aaac
//
LOCUS       DAJTYD010000408          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTYD010000408 DAJTYD010000000
VERSION     DAJTYD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.413"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..194)
                     /locus_tag="OO585_004121"
     CDS             complement(<1..194)
                     /locus_tag="OO585_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6631503.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELT"
     gene            complement(187..>434)
                     /locus_tag="OO585_004122"
     CDS             complement(187..>434)
                     /locus_tag="OO585_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3390 domain-containing protein"
                     /protein_id="HCR6631504.1"
                     /translation="TAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPL
                     KFGAISDWMEARDLPEADGESFRSWFKKHQAQEKKNG"
BASE COUNT           94 a          121 c           99 g          120 t
ORIGIN      
        1 gtcagctcac agcgcgtcaa cataacatcg ctggcaaact gaataaacgc gtcacagcgc
       61 tgctgttggt taagttgggt aagccgctcg ttagcatagt tgttaagcgg cgcatcttct
      121 gcttgcggtt caagtcgcag cgggcgaccc agtgcctgag caacgttatt caaaaattcg
      181 cctcgattat ccattctttt tctcctgcgc ctgatgtttc ttaaaccaac tacggaaact
      241 ctctccgtca gcttcaggaa gatcgcgtgc ttccatccag tcgctaatcg cgccaaattt
      301 gagtggtgtt ttgccgccat tgataaacca gcttgccgca tgagcgccgg ccatcatccc
      361 gactttccac aatcctggat gactattggc ataagcgaac atttttatcg cccgttgctc
      421 tgcttttgcg gtga
//
LOCUS       DAJTYD010000409          433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTYD010000409 DAJTYD010000000
VERSION     DAJTYD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.414"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>433)
                     /locus_tag="OO585_004123"
     CDS             complement(<1..>433)
                     /locus_tag="OO585_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6631505.1"
                     /translation="LIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSL
                     PVLPELLETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLT
                     DIPYLPENLRNGYFDINQISHIPESILNLRNECSIDISDNPL"
BASE COUNT          134 a           65 c          100 g          134 t
ORIGIN      
        1 caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa tgctttccgg
       61 gatatgactt atctgattta tgtcaaaata accgttcctt aaattttccg gcaggtatgg
      121 aatatcggtt aacatattgt cgattgccca tatggagtga atgttgtgag gtagaggggg
      181 gagttcacta agaagattcc ctattgcgct aatctcttgt aaagaaaatg gtaaaggtgg
      241 taaaactgcc agcccattac atgatacatc taatgtttcc agtagttctg gtaaaacagg
      301 aagagaacat aattgattat ttgagacatg aagctccttc aaagatatgg ggagtgtggg
      361 tagtgtgatt agttgattgt gggacgcatt caataattta agtccttgag gcaaagcagg
      421 cagttcaata agt
//
LOCUS       DAJTYD010000410          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTYD010000410 DAJTYD010000000
VERSION     DAJTYD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.415"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
BASE COUNT          149 a           43 c           79 g          159 t
ORIGIN      
        1 gtccattgta ttattggtga tttatgttgc tttacattaa tgacagtgat gtgtgtcgat
       61 taaatattgt tttaagcatt catcataaaa atgtatcatt cgtttcaaaa gatgtctgtg
      121 tttgtatgaa tatattattt tcgtggattc taatgatgat tattaataaa tgtcaaatat
      181 gaaagaaacc atgtctggga aaaaatttac agataattta ttgtatgaat atgatgatta
      241 aaatttcaca attaaatccg aaaaaaagag acgtaactgc ttgttaatta ataaatgata
      301 tcttctatat atttttattg tttataggtg gctatttcgg taaaaatgca ttttcagaat
      361 atcaaccagc ggtaaatcga cgtaaaggga gtgaattgtt tggtaattag gcgagtagac
      421 aggagagtcc
//
LOCUS       DAJTYD010000411          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTYD010000411 DAJTYD010000000
VERSION     DAJTYD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.416"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..344
                     /locus_tag="OO585_004124"
     CDS             <1..344
                     /locus_tag="OO585_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR6631506.1"
                     /translation="RAHNGGIIGPGAVDFIEGFRADYLITSIGAIEHDGTLLEFDLNE
                     ALVARTMIKHARNTLLVADHTKFAASAAVSIGNARNVRAFFTDAPPPNSFCQLLSEEN
                     VELVVAEQEVS"
BASE COUNT          111 a           89 c          105 g          116 t
ORIGIN      
        1 tacgcgctca taacggcggg attatcggcc caggagccgt ggattttatt gaaggcttcc
       61 gcgcagatta tttaatcacc agtataggtg ccattgaaca tgacggcacc ctactggaat
      121 ttgatctaaa tgaagcgtta gtcgcgagaa cgatgattaa acatgcgcgg aatacattgt
      181 tagtggccga tcatacgaag tttgccgcgt ctgctgccgt ttcaattggc aatgcacgga
      241 atgtcagggc tttttttact gatgccccgc ctcccaattc tttctgccag ttgttaagtg
      301 aagagaatgt tgaactggtg gttgccgagc aagaagtatc ctgaaattga aatttcggaa
      361 aatccgctgg gaatttcagc ggattttgtt ttccataagc aaaaaaaacc tgccagcgat
      421 g
//
LOCUS       DAJTYD010000412          416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTYD010000412 DAJTYD010000000
VERSION     DAJTYD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.417"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(2..270)
                     /locus_tag="OO585_004125"
                     /pseudo
     CDS             complement(2..270)
                     /locus_tag="OO585_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          116 a           56 c           78 g          166 t
ORIGIN      
        1 ttcagaaatc agaataccgt tggctttgtt tttcatcaat tccggcttca tttatctttg
       61 tcaaccattg tatttctgta gctgtgaatt tcaacttttt cgttcggttg gtagcaggta
      121 tttgcaattc gtatatggct gtttttgttt attaaaaaca gtacatccat ctgtagtaac
      181 agtagaagat actaatatag tatttgatac ttcagggatg atattttctt gttttttggg
      241 aagacaaggg aatatagttt gtgtaagcat attacttcct tatgttatat ttcggtaaaa
      301 aatttatttt tttacattat agtcggctaa atgaagttta ctgtaaaaaa acgatacttt
      361 ttcgggtggc gcttccaatc agtagaatat gtgtttttca atgaaggagc cgatga
//
LOCUS       DAJTYD010000413          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTYD010000413 DAJTYD010000000
VERSION     DAJTYD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.418"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..248)
                     /locus_tag="OO585_004126"
     CDS             complement(<1..248)
                     /locus_tag="OO585_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6631507.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFR"
BASE COUNT          106 a          109 c          106 g           94 t
ORIGIN      
        1 tggcggaaaa tcgtgttgag gccaacgccc ataatgcggg cggttgcccg gcatccaacg
       61 ccattcatgg ccatatcaat gattttctgg tgcgtaccgg gttgagaagc ggtgtaagtg
      121 aactgcagtt gccatgtttt acggcagtga gagcagagat agcgctgatg tccggcggtg
      181 cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca
      241 gaagccactg gagcacctca aaaacaccat catacactaa atcagtaagt tggcagcatc
      301 accagcttct gctacctgac catcataatc acgcagcttc agtgaagcag tgaccacctg
      361 tttaacccgg tgtatcgtta tgatcttatc cagcaattga accgccccgg gtttc
//
LOCUS       DAJTYD010000414          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTYD010000414 DAJTYD010000000
VERSION     DAJTYD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.419"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
BASE COUNT          144 a           62 c           59 g          148 t
ORIGIN      
        1 cgcaggatga gactatacat cgtcgagatc gcaatgatga tatgagttgt tcctcctgcg
       61 acggctctaa tcaaaaaaga gcaggagtgc ttttccatct attgcgatga tggctttatt
      121 atctgaagga tgacagtcac atttaaaaaa tatttaatct atagatcaca atgtagggtc
      181 atggaatata tgtatacaac tattttcttc ttcactttta attatcgtat tcattataaa
      241 actaagttaa aaaaactcct ggagagtgag ttattaatat atttttaaat aaaattaagt
      301 tatcattcat cgatgcattt tatagaatca tcaatggcca taatagtact attaactctc
      361 aattttatat ttttatcatc actacaaata ttaactgaat ttatatcaat gat
//
LOCUS       DAJTYD010000415          406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTYD010000415 DAJTYD010000000
VERSION     DAJTYD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.420"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>406
                     /locus_tag="OO585_004127"
     CDS             <1..>406
                     /locus_tag="OO585_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6631508.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGV"
BASE COUNT           95 a          112 c          119 g           80 t
ORIGIN      
        1 gcgtgggtgc gtttttggcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggca cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgt
//
LOCUS       DAJTYD010000416          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTYD010000416 DAJTYD010000000
VERSION     DAJTYD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.421"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>404
                     /locus_tag="OO585_004128"
     CDS             <1..>404
                     /locus_tag="OO585_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6631509.1"
                     /translation="QLSRPSEINRLKALVAKLQRMQFGKSSEKLRAKTERRIQEAQER
                     ISALQEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVIRPEEECCPACGGEL
                     SPLGCDVSEQLELISSAFKVIEKQRPKLACRR"
BASE COUNT          101 a          115 c          113 g           75 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtg
//
LOCUS       DAJTYD010000417          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTYD010000417 DAJTYD010000000
VERSION     DAJTYD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.422"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(132..>402)
                     /locus_tag="OO585_004129"
     CDS             complement(132..>402)
                     /locus_tag="OO585_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6631510.1"
                     /translation="TEEWVSCGKIHADIRGRSSRERMQSGAEMAQAEIRIWVRGQSGR
                     EITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRLGGEK"
BASE COUNT           77 a          152 c           89 g           84 t
ORIGIN      
        1 cacttcttct ttcagcacat tcgcacccgc acgggttgcc tcacgcagca cccggttatt
       61 ttccgcacca ctcagaagct gcaaatcgcg gctgatgtcc tccagccccg aaaaatccag
      121 caggatttcg atcatttttc ccctcccagc cgacagagaa tttccagacg cccgccggtc
      181 gcatccggca cgggcagccc gacaacgttc aggatccggt cacgccatgg accactcagc
      241 acatgaagtc gtgacgctgc cgtgatttcc cgaccggact gaccgcgcac ccagatgcgg
      301 atttccgcct gcgccatttc cgcaccggac tgcatccgct cccggctgct cctgcctcgg
      361 atatccgcat gaattttccc gcatgacacc cattcttccg tc
//
LOCUS       DAJTYD010000418          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTYD010000418 DAJTYD010000000
VERSION     DAJTYD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.423"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(52..>401)
                     /gene="speG"
                     /locus_tag="OO585_004130"
     CDS             complement(52..>401)
                     /gene="speG"
                     /locus_tag="OO585_004130"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR6631511.1"
                     /translation="VELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVL
                     NLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAIRMCIFQHQYLAEH
                     KTPGQTLLKPTAQ"
BASE COUNT          109 a           93 c           85 g          114 t
ORIGIN      
        1 cgccgtcgaa cgggtttaca ccatcaaaaa tacgatcgat tattattaat gctattgtgc
       61 ggtcggcttc aggagagtct gacccggtgt tttgtgctct gccagatact gatgctggaa
      121 tatacacatg cgaatggcat tacgatattg accattaata aaaaactcat gcatcaattc
      181 accttcaacc gtaaagccaa gcttgcggta aatgtgaatc gctttttcat tctctttatc
      241 gacgatcaga tacagcttat agagattgag aacggtgaag ccatagtcca ttgctaattt
      301 ggcggcacgg gttgccagac ctttcccctg atactccggg gagataatta tctgaaattc
      361 tgcccggcga tgtacatggt taatttccac cagctccacc a
//
LOCUS       DAJTYD010000419          399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTYD010000419 DAJTYD010000000
VERSION     DAJTYD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.424"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>399)
                     /locus_tag="OO585_004131"
     CDS             complement(<1..>399)
                     /locus_tag="OO585_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6631512.1"
                     /translation="LPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEATGLYQVPLSSAQPGDV
                     LLCCFGSSVPNHAAIYCGDGELLHHIPEQL"
BASE COUNT           99 a          136 c          100 g           64 t
ORIGIN      
        1 ctcagttgtt caggaatatg gtgcagcagc tcaccgtcgc cgcagtaaat ggcggcatga
       61 ttcggcaccg atgaaccaaa acagcacagc agcacatcgc ccggttgtgc tgatgacaac
      121 ggcacctgat acagccctgt ggcctccaga ttatccagat agagattctg accgtgacgc
      181 caccagtcat ccccgcgatg aaaatccggc atctcaatcc ccgccagatg ataagcatcc
      241 cggaacagcg tgtaacagtc cgtcaccccg tgctcaaagc gccgcccggt gagatgcggc
      301 acacagcgga acttgtgaat cgccccccgg cagaccagcc accacggcaa atcactctgc
      361 acctgcagcc gccggtcagc ctcactcagc cagggcaga
//
LOCUS       DAJTYD010000420          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTYD010000420 DAJTYD010000000
VERSION     DAJTYD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.425"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>398
                     /locus_tag="OO585_004132"
     CDS             <1..>398
                     /locus_tag="OO585_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HCR6631513.1"
                     /translation="TGEIMFFVKVGSRFPLLGELQSILKQAVEEATVKAPLRHNAVEI
                     FDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPGRSKVLMPSEGYEGVV
                     KFVFENISTLAVNACPPVLVGVGIATSVETA"
BASE COUNT          100 a          109 c          110 g           79 t
ORIGIN      
        1 accggggaga ttatgttctt cgttaaagtc ggttcccgct tcccactgct tggcgagctg
       61 caaagcatac tcaaacaagc cgtggaagag gcaaccgtca aagcgccact acgtcacaat
      121 gcggtagaaa tttttgacga agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg
      181 gtcacctggg acatcatccc cgacaatgac gatgcggaaa tcgaagttta catggcaggt
      241 ggcggctgca cgctacctgg ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc
      301 gtggtgaaat tcgtcttcga aaatatctcc accctcgccg taaacgcctg tccaccggta
      361 ctggtgggcg tgggcatcgc cacctcggtg gaaaccgc
//
LOCUS       DAJTYD010000421          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTYD010000421 DAJTYD010000000
VERSION     DAJTYD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.426"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>397
                     /locus_tag="OO585_004133"
     CDS             <1..>397
                     /locus_tag="OO585_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR6631514.1"
                     /translation="EIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVT
                     TRSAKSHTAWGKAFRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVER
                     LDYSKGLPERFLAYEALLEKYPQHHGKIRY"
BASE COUNT          114 a           98 c           91 g           94 t
ORIGIN      
        1 ggaaatcttc aacgcgctgc cgacatatga caccttgctt gaacagcttt gtgattatga
       61 tttgctgggt ttccagacag aaaacgatcg tctggcgttc ctggattgtc tttctaacct
      121 gacccgcgtc acgacacgta gcgcaaaaag ccatacagcc tggggcaaag catttcgaac
      181 agaagtctac ccgatcggca ttgaaccgaa agaaatagcc aaacaggctg ccgggccact
      241 gccgccaaaa ctggcgcaac ttaaagcgga actgaaaaac gtacaaaata tcttttctgt
      301 cgaacggctg gattattcca aaggtttgcc agagcgtttt ctcgcctatg aagcgttgct
      361 ggaaaaatat ccgcagcatc atggtaaaat tcgttat
//
LOCUS       DAJTYD010000422          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTYD010000422 DAJTYD010000000
VERSION     DAJTYD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.427"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..144
                     /locus_tag="OO585_004134"
     CDS             <1..144
                     /locus_tag="OO585_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6631515.1"
                     /translation="YTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIK
                     HYQ"
     gene            162..>396
                     /locus_tag="OO585_004135"
     CDS             162..>396
                     /locus_tag="OO585_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6631516.1"
                     /translation="MANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELM
                     ESMMGISLNHGVHAKVSTPVHLRKARTCYDHLAG"
BASE COUNT          117 a           83 c           95 g          101 t
ORIGIN      
        1 tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga
       61 cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
      121 ctgaacataa aacactatca ataagttgga gtcattaccc tgtggcgaat atatcagctt
      181 caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca gtagtagctc
      241 aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg atggaaagta
      301 tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca gtgcatttac
      361 gaaaagcacg tacttgctat gatcatttag ctggcg
//
LOCUS       DAJTYD010000423          394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTYD010000423 DAJTYD010000000
VERSION     DAJTYD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.428"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(39..365)
                     /locus_tag="OO585_004136"
     CDS             complement(39..365)
                     /locus_tag="OO585_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR6631517.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVLREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
BASE COUNT           83 a          137 c           87 g           87 t
ORIGIN      
        1 tatgtcagac gacctgcaga tattgccatt ccccctcctc ataacaccgt cggacaacgc
       61 aaatcgtaaa tcagcacgga aacagaaaac ggcagctccc cctgaagcag ttcttcccgc
      121 tccgcaagat ccggattccg gtacagcatc ccggtcagtc gcatggcagc ccccttcatc
      181 cgggttaatg cctcgcccgg gatcagttca ccgtcctcac ggagcacttt atcccggctg
      241 ccctgaatgt aggccagcag cacggcggta gcctgacgaa ccttgtccat cagcatgtca
      301 tcatccgcgt catggtcgac acgcagatgt gccttgatct cttccagtgt cagtaatgcc
      361 gtcattttcc gcctcctgca tcccgcccac gttt
//
LOCUS       DAJTYD010000424          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTYD010000424 DAJTYD010000000
VERSION     DAJTYD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.429"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            148..>391
                     /locus_tag="OO585_004137"
     CDS             148..>391
                     /locus_tag="OO585_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6631518.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGL"
BASE COUNT           91 a           83 c           95 g          122 t
ORIGIN      
        1 cctagctctt tcagcgcttt agcgacttct tccaactgca tatgacaccc ctctgtctgg
       61 ttgatatttc gataatacca gaaatatctt gatctgaatt tcatttcgat tattctggaa
      121 atatagattt atccaatgag gttacgtatg agtagttggc ttgcgatgct gcaagatgcc
      181 gcagagatgt ttgtgtttct cgccgtcgag ctttcgttgc tgtttatagt gattagtgcc
      241 ggtgtcagcc tgataagaca aaaggtgcca gaccataaaa tccagcagat gatgggggcc
      301 agaaaaggga gaggctatct cctggctgct ctgttgggag ccgttacccc gttctgtagt
      361 tgctcgacaa tacccatgtt acgtggattg t
//
LOCUS       DAJTYD010000425          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTYD010000425 DAJTYD010000000
VERSION     DAJTYD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.430"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..327)
                     /locus_tag="OO585_004138"
     CDS             complement(<1..327)
                     /locus_tag="OO585_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631519.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFV"
BASE COUNT          114 a           87 c           95 g           93 t
ORIGIN      
        1 tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca gcacgtcgat
       61 atcttcatag cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa gcaggataaa
      121 taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca tcaccgccag
      181 ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac ggataaaact
      241 gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact caatcgtttc
      301 ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca actttccagg
      361 gaacacccta ttttaacgtg ggaatagta
//
LOCUS       DAJTYD010000426          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTYD010000426 DAJTYD010000000
VERSION     DAJTYD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.431"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>388
                     /locus_tag="OO585_004139"
     CDS             <1..>388
                     /locus_tag="OO585_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6631520.1"
                     /translation="QLSPMNKVTAKSFTIDSLTRLEEKKFPVGESESKFNQVNIINQG
                     EDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQLGSGEWSLIAESIDPSA
                     VRQFIIQYNIAMQKQLAAHPELANDEV"
BASE COUNT          131 a           73 c           84 g          100 t
ORIGIN      
        1 caattatcac caatgaacaa agtaacggca aaaagtttta ccattgattc tctgacacga
       61 ttagaagaaa agaaatttcc ggttggtgaa agcgagtcga aatttaatca ggttaatatt
      121 atcaatcagg gggaagacgt tgcccaaatc gatgctttcg ttgcaaaaac caggctggat
      181 cgcgttaaag acaaagatta tatcaatgtc aatctgacct acgaacttga taagttaaca
      241 aaagggaatc agcaactcgg tagtggtgag tggtcattga ttgctgaatc tattgatccc
      301 tcagcagtgc gccaatttat catccagtat aacattgcga tgcagaagca gcttgctgca
      361 caccctgagt tagcaaacga tgaagttg
//
LOCUS       DAJTYD010000427          386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTYD010000427 DAJTYD010000000
VERSION     DAJTYD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.432"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..306
                     /locus_tag="OO585_004140"
     CDS             <1..306
                     /locus_tag="OO585_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6631521.1"
                     /translation="TAQNIMQANANLNGLFGFGDDAALAAVIAAKSAHNDNIKIIGFD
                     GMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNGKPVEKMIPVQPGVYTGK"
BASE COUNT          112 a           81 c           94 g           99 t
ORIGIN      
        1 actgcgcaaa acattatgca ggcaaatgcc aacctgaatg gtctgtttgg ctttggtgat
       61 gatgcggcgc tggcagcggt tatcgcagca aaatctgcgc ataacgacaa catcaaaatc
      121 attggttttg atggcatgaa agaagcgcgt gatgccgttg acagtgaaaa aacgtttgcg
      181 gccgtgattc ggcaatatcc cgatcaaatg ggtgctaaag ccattgatgc cgctgttgat
      241 cacctcaatg gtaaaccggt agaaaaaatg atcccggtac aacccggcgt ttatacaggt
      301 aaatagtaaa catcttcacc tcacaacaaa acgttattgg cgttttgttg tgacaccgag
      361 gtcattatga cggatgttat tcttga
//
LOCUS       DAJTYD010000428          383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTYD010000428 DAJTYD010000000
VERSION     DAJTYD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.433"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>383)
                     /locus_tag="OO585_004141"
     CDS             complement(<1..>383)
                     /locus_tag="OO585_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6631522.1"
                     /translation="ATEEHVRKAIAGYGVALDLTLRDVQGKMKKAGQPWEKAKAFDNS
                     CPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQGTTADMIHKIVPLIAYMSKFFTLKAG
                     DVVLTGTPDGVGPLQSGDELTVTFDG"
BASE COUNT           84 a          112 c           93 g           94 t
ORIGIN      
        1 ccatcgaaag tgactgtcag ctcatcaccg ctttgcaacg ggccgacgcc atcaggcgtg
       61 cctgtcaaca caacgtcacc ggccttgagg gtaaaaaact tgctcatata agcgatcaac
      121 ggaacgattt tatggatcat gtccgcagtc gtaccttgct ggcgttgttc gccgtttacg
      181 ctcaggccca gcgttgtatt ttgtggatcg ccggtaaatt ccgccgcggg gataaacccg
      241 gaaagcggac aagagttatc aaacgcttta gccttttccc acggctgccc ggctttcttc
      301 atttttccct gaacatcacg caatgtcaga tcaagcgcca cgccgtaacc ggcaatggct
      361 ttgcggacat gctcttccgt tgc
//
LOCUS       DAJTYD010000429          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTYD010000429 DAJTYD010000000
VERSION     DAJTYD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.434"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            234..>377
                     /locus_tag="OO585_004142"
     CDS             234..>377
                     /locus_tag="OO585_004142"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Involved in the tartrate degradation pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6631523.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSM"
BASE COUNT          116 a           73 c           80 g          108 t
ORIGIN      
        1 tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg
       61 tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt
      121 gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag
      181 tggtagttta tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga
      241 gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct aactttaccg
      301 ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg
      361 ccgaaacgtc gtcgatg
//
LOCUS       DAJTYD010000430          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTYD010000430 DAJTYD010000000
VERSION     DAJTYD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.435"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            176..331
                     /locus_tag="OO585_004143"
                     /pseudo
     CDS             176..331
                     /locus_tag="OO585_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          117 a           82 c           93 g           82 t
ORIGIN      
        1 aacgggatct atacttttgc gggtcaacat aagaggatga gtccttaata aaacaggtcc
       61 tcagagaata catattctgc ggagcgtgcg gcaacaacag gacgaacagg ggcggttttc
      121 aatttccagc cgtcaggctg ccgtggttca ctccaacaac ggagacaggt aaattatgga
      181 cgaaaagaag cttaaagccc ttgcggctga actggctaaa ggcctcaaaa ccgaggccga
      241 cctcaatcag ttttcccgta tgcagacgaa attaactgtc gaaacggtgc ttaatgagga
      301 actaactgac catctcgggc atgaaaaaag ctacatccgg tagtaaacat tatcagtggg
      361 ttatgcgata tgat
//
LOCUS       DAJTYD010000431          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTYD010000431 DAJTYD010000000
VERSION     DAJTYD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.436"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..345)
                     /locus_tag="OO585_004144"
     CDS             complement(<1..345)
                     /locus_tag="OO585_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6631524.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQG"
BASE COUNT          112 a           80 c          105 g           74 t
ORIGIN      
        1 accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg cgcagaacag
       61 tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc caaacatatc
      121 gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg cgttcactac
      181 ccagatggaa ctggctggcg tggcatgaag gtccgtggcg attgttggca gggcgacgtt
      241 ggcgattgcg ccgtcaagga cagccatcga aataccaatc acaatggtta atatcgcacc
      301 gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa ttttatatct
      361 ttggtgaata a
//
LOCUS       DAJTYD010000432          364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTYD010000432 DAJTYD010000000
VERSION     DAJTYD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.437"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>364)
                     /locus_tag="OO585_004145"
     CDS             complement(<1..>364)
                     /locus_tag="OO585_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR6631525.1"
                     /translation="AMILPLGPQNAFVMNQGIRRQYHIMIALLCAISDLVLICAGIFG
                     GSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAEVLKQGRWKIIATM
                     LAVTWLNPHVYLDTFVVLG"
BASE COUNT          114 a          104 c           76 g           70 t
ORIGIN      
        1 gcccagtaca acaaaagtat ccaggtaaac atgcggattc agccaggtca ctgccaacat
       61 ggtggcgata attttccatc tgccttgctt caggacttcg gcactagcta actcaatatt
      121 actactcatc gctgttttaa aagcgccaaa accataccag agcaagaaga ctacgccgcc
      181 ccaggtgacc agcgccagca accacggcga ctgcatcaat aacgcgctac caccaaaaat
      241 ccccgcgcaa atcaggacca aatcgctgat agcgcaaagt aaggcaatca taatgtggta
      301 ctgacgacga atgccctgat tcatcacaaa agcattttgt gggccgagcg gcagaatcat
      361 agct
//
LOCUS       DAJTYD010000433          362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTYD010000433 DAJTYD010000000
VERSION     DAJTYD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.438"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>362)
                     /locus_tag="OO585_004146"
     CDS             complement(<1..>362)
                     /locus_tag="OO585_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6631526.1"
                     /translation="LQVSIIAITAHGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPI"
BASE COUNT          127 a           88 c           72 g           75 t
ORIGIN      
        1 gaataggtat atagagaaat tctccattcc ccagtgccag aaaggcatca tctttaatca
       61 tgatgggcat accatcggtc actatataag cgataccacg gaagatcccc atagtagcaa
      121 cggtgacaat aaacgacggg atccccgcta tagccgtcaa gccaccattt atcatcccca
      181 gagccagtgc tgcaataatg gataatggga ccaataaaat aaatggaatt ccccatgaag
      241 ccccaagcgc agcaaatgtg ccaatcaatg caatggttga accgaccgat aaatcaatat
      301 cgccaagcaa aagtacataa gtcattccat gggcggtaat tgcaattatc gaaacctgaa
      361 ga
//
LOCUS       DAJTYD010000434          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTYD010000434 DAJTYD010000000
VERSION     DAJTYD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.439"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>360
                     /locus_tag="OO585_004147"
     CDS             <1..>360
                     /locus_tag="OO585_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6631527.1"
                     /translation="IHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLC
                     KLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQ
                     RVLAHPLYPVLTSMPGV"
BASE COUNT           77 a          115 c           97 g           71 t
ORIGIN      
        1 agatacatcc ggcactggag cgcgttctcg gtccgagact tgagcacccg gcggtactcg
       61 atcttctcca gcgatatccc tcaccagaaa aactcgcttc gctgggtgag aagaagctgg
      121 cagcccagct ctgcaaactt gcgcctcgtc tgggtaaacg ccttgcagca gacatagctc
      181 aggcactggc cgaacaaacc gtcgtcgttc ccggcacgaa tgccgctgcc gtagtactgc
      241 cacgtctggc actccagctc atcacgctgc gtaagcaaag agacgaggtg gcgcttgagg
      301 tagaacagcg agttcttgct caccctcttt acccggtcct gaccagtatg cccggagtcg
//
LOCUS       DAJTYD010000435          358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTYD010000435 DAJTYD010000000
VERSION     DAJTYD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.440"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..314)
                     /locus_tag="OO585_004148"
     CDS             complement(<1..314)
                     /locus_tag="OO585_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6631528.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRL"
BASE COUNT           99 a           86 c          106 g           67 t
ORIGIN      
        1 aggcgctggc gggcctgaat aacggcactg ggggcaacga aggggcgatt gcccggcagc
       61 atgatgtcca ggcgattcac aatctggtga agaggttctt tacgctcaag cgccatgcca
      121 acaatacacc agaccatcat ttcgagggga agacggcgct tgcgtagcgt tacagtacct
      181 gattcggcaa ggcaacgaga gatgagttcg gggtcgaggt aatcccccag agaagtcagt
      241 gggttacgca gagaatcgta acgggatacc agatcaagag cctgtccaat gtgcataaaa
      301 aaatccggaa acaagtgagc gtttccggat tcttacacag ccactggatc ggtcaact
//
LOCUS       DAJTYD010000436          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTYD010000436 DAJTYD010000000
VERSION     DAJTYD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.441"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(1..354)
                     /locus_tag="OO585_004149"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="OO585_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
BASE COUNT           88 a          102 c           81 g           83 t
ORIGIN      
        1 cggcgacgag tttgtcgcgc tgctgttgag ataacgtagc agaaagcaaa ataacgctgt
       61 ttccgccacg cgcctgccgc tcgatcagcc cttcaagaat gcacgacata taagcgtcac
      121 aggcgtggat ctcatcagcc agcaggattt tgttactcag ccccagaagc cgcagattat
      181 tatgtttaaa cggcatcact gccatcatcg cctgatccag ggtgccgacg ccaatttcag
      241 ccagcaacgc cttcttgtta ttgtcggcaa actaggccgc acaaccctgg ctgaaagctt
      301 gttcatccgg ttcttctgac ccgattaaat caccggacca gagtgattca ttaa
//
LOCUS       DAJTYD010000437          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTYD010000437 DAJTYD010000000
VERSION     DAJTYD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.443"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>344
                     /locus_tag="OO585_004150"
     CDS             <1..>344
                     /locus_tag="OO585_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6631529.1"
                     /translation="RPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAARAFILGDSAQ
                     NISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISGDAGLTIQGG
                     ASTTEDQIKYGW"
BASE COUNT           78 a           91 c           91 g           84 t
ORIGIN      
        1 agcgtccgcc tgctgctgtg ttatacgccg atgaactggc aaaattaaaa caaaatgata
       61 acgcaccttg cccgcccggt tggcagttaa gtttgcctgc ggcccgtgct tttatccttg
      121 gcgatagcgc gcaaaatatt agccgtaaag tggtgattag tccctccgct gtcgaacgta
      181 tgttagtcac tcttgccacc ggacgcggtt tgatgttggt gggggaacct ggcaccgcaa
      241 aatctctcct gtctgaatta ctggcaaccg ctattagcgg cgatgccggg ttaaccatcc
      301 agggcggcgc atctactacc gaagatcaaa tcaagtacgg ctgg
//
LOCUS       DAJTYD010000438          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTYD010000438 DAJTYD010000000
VERSION     DAJTYD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.444"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(201..>341)
                     /gene="fumD"
                     /locus_tag="OO585_004151"
     CDS             complement(201..>341)
                     /gene="fumD"
                     /locus_tag="OO585_004151"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6631530.1"
                     /translation="EMRDLGQEPKHIVIAGVLRTALANKRIQRSELDKQAMETVINAL
                     VK"
BASE COUNT          104 a           68 c           59 g          110 t
ORIGIN      
        1 tattatcatt tgaaataaat ttaacttaag cgacaattat ttgtgacgtt cattgcagaa
       61 aataaaaata atattatcgt tataaattaa aatacatacc gcaattcgta taatttcagc
      121 agttgttatt tagaatgatt aactggaccg caactgaagc aagaatatca ggatgaagct
      181 taccgcctca tcctgaatgg tcattttacc agtgcgttaa tgacggtttc catcgcctgc
      241 ttatccagct cactgcgctg aatgcgttta ttggctaatg ctgtacgtaa tacgcctgcg
      301 ataacaatat gctttggttc ctgccccaga tcgcgcattt c
//
LOCUS       DAJTYD010000439          337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTYD010000439 DAJTYD010000000
VERSION     DAJTYD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.445"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
BASE COUNT          113 a           71 c           60 g           93 t
ORIGIN      
        1 ctgggtaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatttga
       61 aattgatacc tttctaatcg tcacctggat gaaaataatt cattgatatt caaaattaat
      121 ttcctacagg aatgcagagg cggcgggaag atatgccctt aatgccactg aaaaaccaca
      181 taatctgtac agcaataaaa gtcacggcct aatatggcta acaaacaatc aattgtgata
      241 tatttcacaa aattaatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
      301 gatagccaga gtgtaccacg cctcccgtga acaacgc
//
LOCUS       DAJTYD010000440          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTYD010000440 DAJTYD010000000
VERSION     DAJTYD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.446"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>327)
                     /locus_tag="OO585_004152"
     CDS             complement(<1..>327)
                     /locus_tag="OO585_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631531.1"
                     /translation="QIDLTSDLVIDMSTPDKMAIATQHDDGYAASRINASGRMLINGS
                     IFSKGGLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHST
                     VDFASHG"
BASE COUNT           77 a           82 c           84 g           84 t
ORIGIN      
        1 cccgtggctg gcaaaatcga cagttgaatg gctcagcgcc agcgtgtcga gattagaatt
       61 acttgttacg ttccagacgc tgttattcat tgcaacgtcc agttttccgc cattgacatt
      121 atcgctgagg gaggaacctg tccaaaccga cccagggtgc atatccagat tgattagccc
      181 acctttggaa aaaatgctac cgttgataag catacgaccc gaggcgttga tgcggctggc
      241 ggcataacca tcgtcatgtt gcgttgcaat ggccatcttg tcgggtgtac tcatatcaat
      301 gaccagatcg ctcgtgaggt cgatctg
//
LOCUS       DAJTYD010000441          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTYD010000441 DAJTYD010000000
VERSION     DAJTYD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.447"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(51..327)
                     /locus_tag="OO585_004153"
                     /pseudo
     CDS             complement(51..327)
                     /locus_tag="OO585_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
BASE COUNT           83 a           83 c           87 g           74 t
ORIGIN      
        1 ctgctcaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aaccagccag
       61 tcggtaaaca gcgtaaaccc ttccagcatg atgttttcgc gtggcggaat taactcttcc
      121 cactgttgct catcacgcag gcgggtacgg tataagccaa agtttttgcc gtggcggttg
      181 gaacgcagat aaaaccgatg ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc
      241 ggcagaaaaa caaacggctc ggcatcggcc atttccgcgt ccagcaggcg aacttcactg
      301 gtggtggcgc tggccaaatg aatgact
//
LOCUS       DAJTYD010000442          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTYD010000442 DAJTYD010000000
VERSION     DAJTYD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.448"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>326
                     /locus_tag="OO585_004154"
     CDS             <1..>326
                     /locus_tag="OO585_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6631532.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAJTYD010000443          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTYD010000443 DAJTYD010000000
VERSION     DAJTYD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.449"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
BASE COUNT          126 a           43 c           47 g          106 t
ORIGIN      
        1 ctctgctctg tgtctctatt ttatctatca tattctgagg attaactaca taacaaggcg
       61 caagatatat atccaaaaac aaataatacc gttgcaataa ttcctttcag taaaggaggt
      121 gtgattaatt tcaggggatt tcagagtgaa aattgtacaa catattgaaa tatgttcatt
      181 tcgttataat acacacaaga aaatattaag ttagataata tattctaaat aaccatagat
      241 aaaaatgcac ataatgggga atattttatg aaaatacctg aagcagtaaa tcatattaat
      301 gttcagaata atattgatct tg
//
LOCUS       DAJTYD010000444          321 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTYD010000444 DAJTYD010000000
VERSION     DAJTYD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.450"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            52..>321
                     /locus_tag="OO585_004155"
     CDS             52..>321
                     /locus_tag="OO585_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6631533.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEILKKATAL"
BASE COUNT          118 a           73 c           72 g           58 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct c
//
LOCUS       DAJTYD010000445          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTYD010000445 DAJTYD010000000
VERSION     DAJTYD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.452"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..>317)
                     /locus_tag="OO585_004156"
     CDS             complement(<1..>317)
                     /locus_tag="OO585_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="multicopy suppressor of dominant negative ftsH
                     mutations; putative acyl-CoA synthetase; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCR6631534.1"
                     /translation="LKIVNAMKATGKPMVALFLGYTPAVARDENVWFASSLDEAARLA
                     CLLSRVTARCNAIAPVSSGFICGLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDA
                     DGH"
BASE COUNT           73 a           97 c           87 g           60 t
ORIGIN      
        1 gtgaccatcg gcgtcgagca tcatgccgtg atgatgggta tcgtcggctt ccacgccaag
       61 gtgtccagca agtaatcccg ccgcttcggc agccagcgta ccgccggtat acaaaccgca
      121 aataaatccg ctgctgacag gcgctattgc gttacatcgc gccgtgacgc gtgaaagcag
      181 gcaagccagg cgtgcggcct catccagcga ggaggcaaac cagacattct cgtcgcgggc
      241 caccgccggg gtataaccta aaaacagcgc caccatcggt ttgccggttg ctttcatggc
      301 attaacaatt ttcagac
//
LOCUS       DAJTYD010000446          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTYD010000446 DAJTYD010000000
VERSION     DAJTYD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.453"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..>317
                     /locus_tag="OO585_004157"
     CDS             <1..>317
                     /locus_tag="OO585_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6631535.1"
                     /translation="SPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVPEDLLYARF
                     DSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATGGT
                     VAH"
BASE COUNT           68 a           92 c           82 g           75 t
ORIGIN      
        1 tagtcctcgc tttccttctc gctccagctc cttgctggaa tatgcgcggg aaaaactaaa
       61 tggcctggat gtagaggttt atcactggaa tctgcaaaac ttcgtcccgg aagatctgct
      121 ttatgctcgt ttcgatagtc cggcactcaa gaccttcacc gaacagctgc aacaggctga
      181 tggactgata gtcgctacac ctgtgtataa agccgcctat tccggcgcgc tgaaaaccct
      241 gctcgacctg ctgccagagc gcgctttgca aggcaaagtg gtgctaccgc tggcgacggg
      301 cggtaccgtg gcccatc
//
LOCUS       DAJTYD010000447          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTYD010000447 DAJTYD010000000
VERSION     DAJTYD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.454"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..286
                     /gene="bamD"
                     /locus_tag="OO585_004158"
     CDS             <1..286
                     /gene="bamD"
                     /locus_tag="OO585_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6631536.1"
                     /translation="RGYPNSQYTTDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVV
                     NRVEGMLRDYPDTQATRDALPLMENAYRQMQMNAQAEKVAKIIAANSSNT"
BASE COUNT           97 a           79 c           77 g           57 t
ORIGIN      
        1 gcgcggctat ccgaacagtc agtacactac cgatgcaacc aaacgtctgg tattcctgaa
       61 agatcgtctg gcgaaatatg aatactccgt tgccgagtac tatacagaac gtggtgcatg
      121 ggttgccgtc gttaaccgcg tagaaggcat gttgcgcgac tacccggata cccaggctac
      181 gcgtgatgcg ctgccactga tggaaaatgc ataccgtcag atgcagatga atgcgcaagc
      241 tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga aacctgaaac
      301 acaaaacggc
//
LOCUS       DAJTYD010000448          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTYD010000448 DAJTYD010000000
VERSION     DAJTYD010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.denovo.455"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..267
                     /locus_tag="OO585_004159"
     CDS             <1..267
                     /locus_tag="OO585_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6631537.1"
                     /translation="TVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTEDDVVSTDFNGE
                     VCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
BASE COUNT           90 a           72 c           73 g           70 t
ORIGIN      
        1 accgttcgtc tggaaaaagc tgcaacttac gagcagatca aagctgccgt taaagccgct
       61 gctgaaggcg aaatgaaagg cgttctgggc tacaccgaag atgacgtagt atctaccgat
      121 ttcaacggcg aagtttgcac ttccgtgttc gatgctaaag ctggtatcgc tctgaacgac
      181 aacttcgtga aactggtatc ctggtacgac aacgaaaccg gttactccaa caaagttctg
      241 gacctgatcg ctcacatctc caaataagtt gagatgaaac tgtgatctaa aaagagcgac
      301 ttcgg
//
LOCUS       DAJTYD010000449         6801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.guided.187, whole genome shotgun sequence.
ACCESSION   DAJTYD010000449 DAJTYD010000000
VERSION     DAJTYD010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.guided.187"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            980..1795
                     /gene="sul2"
                     /locus_tag="OO585_004160"
     CDS             980..1795
                     /gene="sul2"
                     /locus_tag="OO585_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6631538.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1856..2383
                     /locus_tag="OO585_004161"
                     /pseudo
     CDS             1856..2383
                     /locus_tag="OO585_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2404..2877
                     /locus_tag="OO585_004162"
     CDS             2404..2877
                     /locus_tag="OO585_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6631539.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2952..3227
                     /locus_tag="OO585_004163"
                     /pseudo
     CDS             2952..3227
                     /locus_tag="OO585_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            3227..4063
                     /locus_tag="OO585_004164"
     CDS             3227..4063
                     /locus_tag="OO585_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6631540.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4170..4574)
                     /locus_tag="OO585_004165"
                     /pseudo
     CDS             complement(4170..4574)
                     /locus_tag="OO585_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4618..5007)
                     /locus_tag="OO585_004166"
     CDS             complement(4618..5007)
                     /locus_tag="OO585_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631541.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            5225..5665
                     /locus_tag="OO585_004167"
     CDS             5225..5665
                     /locus_tag="OO585_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6631542.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6421..6570)
                     /locus_tag="OO585_004168"
     CDS             complement(6421..6570)
                     /locus_tag="OO585_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631543.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1673 a         1778 c         1761 g         1589 t
ORIGIN      
        1 tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg
       61 gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa
      121 gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca
      181 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
      241 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      301 gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg
      361 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga
      421 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc
      481 acgagaagat agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa
      541 cagttttggt aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga
      601 cttaaccttc gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta
      661 cgattcattg aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg
      721 aacatgacac cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta
      781 ttggtgactt gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg
      841 atatatttat ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg
      901 ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat
      961 tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac
     1021 ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc
     1081 gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc
     1141 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc
     1201 gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta
     1261 tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt
     1321 ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg
     1381 gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt
     1441 tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga
     1501 tcccggcatg gggttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg
     1561 gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc
     1621 ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc
     1681 agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     1741 gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca
     1801 cattcgggat atttctctat attcgcgctt catcagaaaa ctgaaggaac ctccattgaa
     1861 tcgaactaat attttttttg gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga
     1921 atctggtgat tttgtttttc gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc
     1981 ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc atttggctca aaggtcgagg
     2041 tgtggcttgc cccgaggtca tcaactggca ggaggaacag gagggtgcat gcttggtgat
     2101 aacggcaatt ccgggagtac cggcggctga tctgtctgga gcggatttgc tcaaagcgtg
     2161 gccgtcaatg gggcagcaac ttggcgctgt tcacagccta tcggttgatc aatgtccgtt
     2221 tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt
     2281 caatcccgac ttcttaccgg acgaggacaa gagtacgccg cagctcgatc ttttggctcg
     2341 tgtcgaacta gagctaccgg tgcggctcga ccaagagcgc accgttaacc caggatgaga
     2401 accttgaaag tatcattgat agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca
     2461 gacataccct ggtccgcgaa aggggagcag ctacttttta aagcattgac ctacaatcag
     2521 tggcttctgg tgggtcgcaa gacgtttgaa tctatgggcg cactccccaa taggaaatac
     2581 gcggtcgtta cccgctcagg ttggacatca aatgatgaca atgtagttgt atttcagtca
     2641 atcgaagagg ccatggacag gctagctgaa ttcaccggtc acgttatagt gtctggtggc
     2701 ggagaaattt accgagaaac attacccatg gcctctacgc tccacttatc gacgatcgac
     2761 atcgagccag agggggatgt tttcttcccg agtattccaa ataccttcga agttgttttt
     2821 gagcaacact ttacttcaaa cattaactat tgctatcaaa tttggaaaaa gggttaacaa
     2881 agctatgcaa tcgacggcaa aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga
     2941 ttgatagcga cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg
     3001 accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc
     3061 gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg
     3121 aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg
     3181 aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc
     3241 tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag
     3301 cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc
     3361 tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag
     3421 gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga
     3481 gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc
     3541 ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc
     3601 gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg
     3661 tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga
     3721 actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg
     3781 gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt
     3841 ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc
     3901 ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc
     3961 ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac gcgatctagc
     4021 tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa gcgacttctc
     4081 ctatcccctg ggaacacatc aatctcaccg gagaatatcg ctggccaaag ccttagcgta
     4141 ggattccgcc ccttcccgca aacgacccca aacaggaaac gcagctgaaa cgggaagctc
     4201 aacacccact gacgcatggg ttgttcaggc agtacttcat caaccagcaa ggcggcactt
     4261 tcggccatcc gccgcgcccc acagctcggg cagaaaccgc gacgcttaca gctgaaagcg
     4321 accaggtgct cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc
     4381 ccgcattgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc
     4441 tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt
     4501 tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc
     4561 gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta
     4621 gcggaaagtt cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt
     4681 gcccgtcact cccactctta cattgttcgc agtatctagg tacatgttat tgcgtgcacc
     4741 ttcatgatgt ataatgttat taatgacagc agaaagatcg acagaattaa tattcttcgt
     4801 acctgggaca gttatcgggt gtcggccagt gatgagacca aaggatgcag tataagatga
     4861 accactacta ttatcatggc caccattacc accaccgtta aaactacctc ccgaacttcc
     4921 agagccgcta tgaccatcat tgtaggggtc atttggtccc atgtgaccaa aaccaccgac
     4981 agtaccgcta tttaactctg tactcataaa attatcctct ttgactttta aaaacagcaa
     5041 aaactgtatt tataacaatg tgtttatata cagtattcga aatactacgc ccgtcaaaac
     5101 cacaccgtca acaggttttt tgtgtatttt tcacgcctca cgggcagcct cagacctttt
     5161 gtgttaaaac agtaatatgt tctgctgtta caatacggta cacacaggag acactgtcac
     5221 ttttatggca tggcacacag ttcaagagac actgaaatta acaggaaagt cgcgctcgca
     5281 attatatcgt gacatggcta aaggccttgt cagctatcgc acaggaaaaa atgatcgccg
     5341 ggagttcgag accagtgaac tgattcgggc ctatggcgag ctgcgcactt ctgagacacc
     5401 aaaaaggcac aaggagggac actccaaatc tgtcgttagc caggcggaaa atgctcagct
     5461 agacgtcatt cagcagaaac tggacgctct gcagcagact gttaccctaa tgcttgaaga
     5521 caagatggcg cgggaagcag aaaaccgcct gcgaaatgag gagagggaac aactacaggc
     5581 cgaaattagc cgtctggctg acgcgctggc acaggaaaaa aagcggggat tctggtcgcg
     5641 gctgtttcgc tccggttcag agtaacagtg caggtgactc tgatttacca taacatctgt
     5701 tatcagcacc gcctgagagg ccctcagggc gtttctgacg ggaaaccggt aaaacaggca
     5761 acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg tttttacggc atgcatgagt
     5821 atgcgcacaa gtgcatagcg cggaaattca caccattcag ccggggtaag tcctgtttcc
     5881 ggcgttcttc ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc
     5941 gttgccggcg cagccggaaa acccgctgtc agcacgttca ggaagggggt gatgtacaaa
     6001 tggcacagag gatgaaagtg acgtatgagc tgaaggaatg atgtaaaagt gatgtaaaag
     6061 tgatgtataa gtgatgtaaa agtgatgtat gaactgaagg agcgatgtaa ttctgtagtc
     6121 aggaagaata acccgttcat gcaggcatga acgatacagt gaggaacgct ttttttgtca
     6181 cgatctcccc ttaacctgcg taaatcaccc gccatggttg attatttttc aacctcaaaa
     6241 tggtcataca cctaacgcag ccgcgatttt catcgcaact gcatgaatta aaagaaaaaa
     6301 aaggaatatt tcgttttcac gagtaatacc cgggtattaa aaatccactg taattacatg
     6361 aaaataaaga aataaaaggt aataccggag tattaccacg tacgcgggca agcgaaaaag
     6421 ctataacgct tcccggccga caggcgaccc aaaatccaca ggggcatgca ggttctcttc
     6481 agtaacctgc gccattaaat caaaaaggtc atattcgccg ggaagctgcg tatcagtcga
     6541 atccagttgt tcatcatatt cagatggcat ggggtgatat ccagtagctg agaggttgtt
     6601 agcgggtttc ggggcgcagc cctgaaccag tcatgtagcg ctagcggagt gtatactggc
     6661 ttactatgtt gccacgattg cgaaaatcag tgaagtgctt catgtggcag gagaaaaaag
     6721 gcagcaccgg cgcgtcagca gaatatgcga tacaggagat attccgcttc ctcgctcact
     6781 gactcgctac gctcggtcgt t
//
LOCUS       DAJTYD010000450         6800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.guided.188, whole genome shotgun sequence.
ACCESSION   DAJTYD010000450 DAJTYD010000000
VERSION     DAJTYD010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.guided.188"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            980..1795
                     /gene="sul2"
                     /locus_tag="OO585_004169"
     CDS             980..1795
                     /gene="sul2"
                     /locus_tag="OO585_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6631544.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1900..2382
                     /locus_tag="OO585_004170"
                     /pseudo
     CDS             1900..2382
                     /locus_tag="OO585_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2403..2876
                     /locus_tag="OO585_004171"
     CDS             2403..2876
                     /locus_tag="OO585_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR6631545.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2951..3226
                     /locus_tag="OO585_004172"
                     /pseudo
     CDS             2951..3226
                     /locus_tag="OO585_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            3226..4062
                     /locus_tag="OO585_004173"
     CDS             3226..4062
                     /locus_tag="OO585_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6631546.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(4169..4573)
                     /locus_tag="OO585_004174"
                     /pseudo
     CDS             complement(4169..4573)
                     /locus_tag="OO585_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4617..5006)
                     /locus_tag="OO585_004175"
     CDS             complement(4617..5006)
                     /locus_tag="OO585_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631547.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            5224..5664
                     /locus_tag="OO585_004176"
     CDS             5224..5664
                     /locus_tag="OO585_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6631548.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(6420..6569)
                     /locus_tag="OO585_004177"
     CDS             complement(6420..6569)
                     /locus_tag="OO585_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6631549.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1673 a         1778 c         1761 g         1588 t
ORIGIN      
        1 tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg
       61 gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa
      121 gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca
      181 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
      241 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      301 gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg
      361 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga
      421 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc
      481 acgagaagat agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa
      541 cagttttggt aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga
      601 cttaaccttc gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta
      661 cgattcattg aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg
      721 aacatgacac cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta
      781 ttggtgactt gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg
      841 atatatttat ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg
      901 ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat
      961 tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac
     1021 ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc
     1081 gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc
     1141 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc
     1201 gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta
     1261 tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt
     1321 ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg
     1381 gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt
     1441 tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga
     1501 tcccggcatg gggttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg
     1561 gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc
     1621 ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc
     1681 agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     1741 gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca
     1801 cattcgggat atttctctat attcgcgctt catcagaaaa ctgaaggaac ctccattgaa
     1861 tcgaactaat atttttttgg tgaatcgcat tctgactggt tgcctgtcag aggcggagaa
     1921 tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct
     1981 tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa aggtcgaggt
     2041 gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg cttggtgata
     2101 acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct caaagcgtgg
     2161 ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca atgtccgttt
     2221 gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg caatgccgtc
     2281 aatcccgact tcttaccgga cgaggacaag agtacgccgc agctcgatct tttggctcgt
     2341 gtcgaactag agctaccggt gcggctcgac caagagcgca ccgttaaccc aggatgagaa
     2401 ccttgaaagt atcattgata gctgcgaaag cgaaaaacgg cgtgattggt tgcggtccag
     2461 acataccctg gtccgcgaaa ggggagcagc tactttttaa agcattgacc tacaatcagt
     2521 ggcttctggt gggtcgcaag acgtttgaat ctatgggcgc actccccaat aggaaatacg
     2581 cggtcgttac ccgctcaggt tggacatcaa atgatgacaa tgtagttgta tttcagtcaa
     2641 tcgaagaggc catggacagg ctagctgaat tcaccggtca cgttatagtg tctggtggcg
     2701 gagaaattta ccgagaaaca ttacccatgg cctctacgct ccacttatcg acgatcgaca
     2761 tcgagccaga gggggatgtt ttcttcccga gtattccaaa taccttcgaa gttgtttttg
     2821 agcaacactt tacttcaaac attaactatt gctatcaaat ttggaaaaag ggttaacaaa
     2881 gctatgcaat cgacggcaaa aagcttcgtt cgcttcgcgc actacgcctt tttccgcgat
     2941 tgatagcgac gatatggttg tttgccatgg tgatccctgc atgccgaact tcatggtgga
     3001 ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa cagcagatcg
     3061 ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag cgccagatga
     3121 agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc ccgaccgcga
     3181 acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt catgccgcct
     3241 gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac cttttccagc
     3301 ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaaggg attgaaacct
     3361 atagaagaca ttgctgatga actgcgcggg gccgactatc tggtatggcg caatgggagg
     3421 ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata tgccggggag
     3481 cgaatgctct ctcacatcgt tgccgagcac ggcgactacc aggcgaccga aattgcagcg
     3541 gaactaatgg cgaagctgta tgccgcatct gaggaacccc tgccttctgc ccttctcccg
     3601 atccgggatc gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt
     3661 caaactgact acgtccacgc ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa
     3721 ctgcgtgggc tacatggcga tctgcatcat gaaaacatca tgttctccag tcgcggctgg
     3781 ctggtgatag atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc caatatgttc
     3841 tacgatccgg ctgacagaga cgacctttgt ctcgatccta gacgcattgc acagatggcg
     3901 gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct
     3961 tatgggtgcc tttccgcagc ttggaacgcg gatggagaag aggagcaacg cgatctagct
     4021 atcgcggccg cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc
     4081 tatcccctgg gaacacatca atctcaccgg agaatatcgc tggccaaagc cttagcgtag
     4141 gattccgccc cttcccgcaa acgaccccaa acaggaaacg cagctgaaac gggaagctca
     4201 acacccactg acgcatgggt tgttcaggca gtacttcatc aaccagcaag gcggcacttt
     4261 cggccatccg ccgcgcccca cagctcgggc agaaaccgcg acgcttacag ctgaaagcga
     4321 ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc
     4381 cgcattggag aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct
     4441 ctgccataag cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt
     4501 gctcgggtcg gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg
     4561 ccacatgagg catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag
     4621 cggaaagttc ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg
     4681 cccgtcactc ccactcttac attgttcgca gtatctaggt acatgttatt gcgtgcacct
     4741 tcatgatgta taatgttatt aatgacagca gaaagatcga cagaattaat attcttcgta
     4801 cctgggacag ttatcgggtg tcggccagtg atgagaccaa aggatgcagt ataagatgaa
     4861 ccactactat tatcatggcc accattacca ccaccgttaa aactacctcc cgaacttcca
     4921 gagccgctat gaccatcatt gtaggggtca tttggtccca tgtgaccaaa accaccgaca
     4981 gtaccgctat ttaactctgt actcataaaa ttatcctctt tgacttttaa aaacagcaaa
     5041 aactgtattt ataacaatgt gtttatatac agtattcgaa atactacgcc cgtcaaaacc
     5101 acaccgtcaa caggtttttt gtgtattttt cacgcctcac gggcagcctc agaccttttg
     5161 tgttaaaaca gtaatatgtt ctgctgttac aatacggtac acacaggaga cactgtcact
     5221 tttatggcat ggcacacagt tcaagagaca ctgaaattaa caggaaagtc gcgctcgcaa
     5281 ttatatcgtg acatggctaa aggccttgtc agctatcgca caggaaaaaa tgatcgccgg
     5341 gagttcgaga ccagtgaact gattcgggcc tatggcgagc tgcgcacttc tgagacacca
     5401 aaaaggcaca aggagggaca ctccaaatct gtcgttagcc aggcggaaaa tgctcagcta
     5461 gacgtcattc agcagaaact ggacgctctg cagcagactg ttaccctaat gcttgaagac
     5521 aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg agagggaaca actacaggcc
     5581 gaaattagcc gtctggctga cgcgctggca caggaaaaaa agcggggatt ctggtcgcgg
     5641 ctgtttcgct ccggttcaga gtaacagtgc aggtgactct gatttaccat aacatctgtt
     5701 atcagcaccg cctgagaggc cctcagggcg tttctgacgg gaaaccggta aaacaggcaa
     5761 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
     5821 tgcgcacaag tgcatagcgc ggaaattcac accattcagc cggggtaagt cctgtttccg
     5881 gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg
     5941 ttgccggcgc agccggaaaa cccgctgtca gcacgttcag gaagggggtg atgtacaaat
     6001 ggcacagagg atgaaagtga cgtatgagct gaaggaatga tgtaaaagtg atgtaaaagt
     6061 gatgtataag tgatgtaaaa gtgatgtatg aactgaagga gcgatgtaat tctgtagtca
     6121 ggaagaataa cccgttcatg caggcatgaa cgatacagtg aggaacgctt tttttgtcac
     6181 gatctcccct taacctgcgt aaatcacccg ccatggttga ttatttttca acctcaaaat
     6241 ggtcatacac ctaacgcagc cgcgattttc atcgcaactg catgaattaa aagaaaaaaa
     6301 aggaatattt cgttttcacg agtaataccc gggtattaaa aatccactgt aattacatga
     6361 aaataaagaa ataaaaggta ataccggagt attaccacgt acgcgggcaa gcgaaaaagc
     6421 tataacgctt cccggccgac aggcgaccca aaatccacag gggcatgcag gttctcttca
     6481 gtaacctgcg ccattaaatc aaaaaggtca tattcgccgg gaagctgcgt atcagtcgaa
     6541 tccagttgtt catcatattc agatggcatg gggtgatatc cagtagctga gaggttgtta
     6601 gcgggtttcg gggcgcagcc ctgaaccagt catgtagcgc tagcggagtg tatactggct
     6661 tactatgttg ccacgattgc gaaaatcagt gaagtgcttc atgtggcagg agaaaaaagg
     6721 cagcaccggc gcgtcagcag aatatgcgat acaggagata ttccgcttcc tcgctcactg
     6781 actcgctacg ctcggtcgtt
//
LOCUS       DAJTYD010000451         5472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.guided.210, whole genome shotgun sequence.
ACCESSION   DAJTYD010000451 DAJTYD010000000
VERSION     DAJTYD010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.guided.210"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..1433)
                     /gene="pepD"
                     /locus_tag="OO585_004178"
     CDS             complement(<1..1433)
                     /gene="pepD"
                     /locus_tag="OO585_004178"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6631550.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTEL"
     gene            1694..2152
                     /gene="gpt"
                     /locus_tag="OO585_004179"
     CDS             1694..2152
                     /gene="gpt"
                     /locus_tag="OO585_004179"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6631551.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2244..3488
                     /gene="frsA"
                     /locus_tag="OO585_004180"
     CDS             2244..3488
                     /gene="frsA"
                     /locus_tag="OO585_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6631552.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3546..3947
                     /gene="crl"
                     /locus_tag="OO585_004181"
     CDS             3546..3947
                     /gene="crl"
                     /locus_tag="OO585_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6631553.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3986..5041)
                     /gene="phoE"
                     /locus_tag="OO585_004182"
     CDS             complement(3986..5041)
                     /gene="phoE"
                     /locus_tag="OO585_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6631554.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            5329..>5472
                     /locus_tag="OO585_004183"
     CDS             5329..>5472
                     /locus_tag="OO585_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6631555.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIV"
BASE COUNT         1338 a         1346 c         1353 g         1435 t
ORIGIN      
        1 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
       61 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
      121 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
      181 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
      241 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
      301 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
      361 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcaac cacacctttg
      421 gctacatcgg agttgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
      481 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
      541 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
      601 gatttcagtg cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc ttcacgcggg
      661 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
      721 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
      781 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
      841 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
      901 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
      961 ttgctctgta agccaaaagc accgtccata ccggcttctt cggtcatggt cagcagcact
     1021 tccagcggac cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     1081 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     1141 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     1201 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     1261 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     1321 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgcggaat agaacagatt
     1381 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     1441 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     1501 caacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag
     1561 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     1621 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     1681 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     1741 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     1801 ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     1861 taccgtttgt atttccagct acgatcacga caaccagcga gagcttaaag tgctgaaacg
     1921 cgcagaaggc gatggcgaag gcttcatagt tattgatgac ctggtggata ccggtggtac
     1981 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     2041 ggctggtcgt ccgctggttg atgactatgt tgttgatatt ccgcaagata cctggatcga
     2101 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     2161 acgcctggca ctgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     2221 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     2281 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     2341 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     2401 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     2461 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgatactgtg attggctacc
     2521 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     2581 gtgcggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     2641 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     2701 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     2761 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     2821 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     2881 tgtatgaacg ttattttgcg ccgcgcggca ttgtgatgct gactattgat atgccgtcgg
     2941 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     3001 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     3061 gtttcggcgc taacgttgcc gtgcgtctgg catatcttga atcgccgcgt ctgaaagcgg
     3121 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     3181 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     3241 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctgggacgtc
     3301 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     3361 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     3421 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     3481 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     3541 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     3601 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     3661 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     3721 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     3781 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     3841 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     3901 aaccggcgga tgattttcgt gacgagccgg tgaagttaac ggcgtgagtg aaatgtgccg
     3961 gatgcatcac atccggcaat atctattaaa actgatacgt catcccaatc gcgacaatat
     4021 catcattatt aatattcaat ttgttatcgc tatccagttg gttgatttta taatcaacaa
     4081 atgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttaaccagat
     4141 cttcaccacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     4201 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     4261 agccgccagt tattggcgtc attttgcgag tttcagaata gaaagttgcc agataaatat
     4321 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     4381 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     4441 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     4501 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     4561 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     4621 cggtctgcgc cgaggagtcg ccaccaaatt ccggaaacat atcggtccag gcttccacgt
     4681 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     4741 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctctgcttta ttaccggcaa
     4801 actctgcttc ccaacgacca taaccagtca gttgatcgtt aatttgtgtt tcgcctttaa
     4861 aaccaaaacg gatataactc tggtcgccat ctttactgtc gttatcactc atataatgca
     4921 tggctttaac tttgccatag acatccagtt tattaccgtc tttgttatat atttctgcgg
     4981 cctgtacgga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     5041 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     5101 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaataata ttcttaaaat
     5161 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc
     5221 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
     5281 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
     5341 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
     5401 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
     5461 attgttattg tg
//
LOCUS       DAJTYD010000452         4347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.guided.228, whole genome shotgun sequence.
ACCESSION   DAJTYD010000452 DAJTYD010000000
VERSION     DAJTYD010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.guided.228"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            <1..592
                     /gene="frdB"
                     /locus_tag="OO585_004184"
     CDS             <1..592
                     /gene="frdB"
                     /locus_tag="OO585_004184"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6631556.1"
                     /translation="APDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVE
                     ALANFPIERDLVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCI
                     NCGLCYAACPQFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFV
                     GYCSEVCPKHVDPAAAIQQGKVESSKDFLIATLKPR"
     gene            603..998
                     /gene="frdC"
                     /locus_tag="OO585_004185"
     CDS             603..998
                     /gene="frdC"
                     /locus_tag="OO585_004185"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6631557.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            1009..1368
                     /gene="frdD"
                     /locus_tag="OO585_004186"
     CDS             1009..1368
                     /gene="frdD"
                     /locus_tag="OO585_004186"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6631558.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            1431..2564
                     /locus_tag="OO585_004187"
     CDS             1431..2564
                     /locus_tag="OO585_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6631559.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            2653..3186
                     /gene="blc"
                     /locus_tag="OO585_004188"
     CDS             2653..3186
                     /gene="blc"
                     /locus_tag="OO585_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6631560.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3183..3500)
                     /gene="sugE"
                     /locus_tag="OO585_004189"
     CDS             complement(3183..3500)
                     /gene="sugE"
                     /locus_tag="OO585_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6631561.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3682..3828)
                     /gene="ecnB"
                     /locus_tag="OO585_004190"
     CDS             complement(3682..3828)
                     /gene="ecnB"
                     /locus_tag="OO585_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6631562.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(3939..4064)
                     /gene="ecnA"
                     /locus_tag="OO585_004191"
     CDS             complement(3939..4064)
                     /gene="ecnA"
                     /locus_tag="OO585_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6631563.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(4116..>4347)
                     /locus_tag="OO585_004192"
     CDS             complement(4116..>4347)
                     /locus_tag="OO585_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6631564.1"
                     /translation="CIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATL
                     STGAVVKVPLFVQIGEVIKVDTRSGEYVSRVK"
BASE COUNT         1083 a         1120 c         1119 g         1025 t
ORIGIN      
        1 ggcaccggac ctgagttacc gctggtcctg ccgtatggcg atttgtggtt cctgcggcat
       61 gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt acaccgacgg
      121 tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg tcgatatgac
      181 ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact cccgcaccgc
      241 ggatcagggt actaacatcc agaccccggc gcagatggca aagtatcacc agttctccgg
      301 ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga acccagagtt
      361 catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc gcgaccacgg
      421 taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct gtactttcgt
      481 gggctattgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca ttcagcaggg
      541 caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct aaggagtgca
      601 acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat
      661 tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct gtgtggttca
      721 gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggacgcc tgggcgggat
      781 tcgtcgactt tttacaaaac ccggttatcg tgatcattaa cctgatcact ctggcggcag
      841 ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat atcattgtaa
      901 aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta actgtggttg
      961 ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat gattaatcca
     1021 aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg tggtatgtgg
     1081 agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc actggggctg
     1141 tttccgggtg atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag cttcattggt
     1201 cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac
     1261 cacgcgatgc acgatctgaa aattcacgtg cctgcgggca aatgggtttt ctacggtctg
     1321 gctgctatcc tgacagttgt cacgctgatt ggtatcgtta caatctaacg tatcgccaat
     1381 gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagacctt atgttcaaaa
     1441 cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc cctcaacaaa
     1501 tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta
     1561 tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca
     1621 tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa
     1681 catttacggg cgtgcttggt ggcgatgcta ttgctcgagg ggaaatcaag ttaagcgatc
     1741 ccgcaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac
     1801 atcttgcgac ctacaccgct ggcggcctgc cattgcagat gccggatgaa gtgaaatcct
     1861 caagcgactt gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac
     1921 gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt
     1981 tgagttttga gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt
     2041 ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc gaaggcaagg
     2101 cagttcatgt ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg
     2161 aagatatggc ccgctgggtg caaagcaatt taacccccct tgatatcaat gagaaaacgc
     2221 ttcaacaagg gatacaactg gcacaatctc gctactggca aaccggcgat atgtatcagg
     2281 gcctgggctg ggaaatgctg gactggccgg tcaatcctga cagcatcatt aacggcagtg
     2341 acaataaaat tgcactggca gcacgcccgg taaaagcgat taagccccca actcctgcag
     2401 tacgcgcatc atgggtacat aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt
     2461 ttattccaga aaaagagctg ggtatcgtga tgctggctaa caaaaactat cccaatccag
     2521 cgagagtcgc cgccgcctgt cagattctca acgctctaca gtaaaatttc atcgggtccg
     2581 aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa aaaaaccagt
     2641 aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc atttctggtc
     2701 gttgcctgca gttctcctac gccgccgcgt ggcgtgacag tggtgaataa tttcgacgct
     2761 aaacgctatc tcggtacctg gtatgagatt gcccgttttg atcaccgttt tgaacgtgga
     2821 ctggaaaaag tcaccgcaac atacagtctg cgtgatggcg gcggcctgaa tgtcatcaat
     2881 aaaggctata accctgacag agaaatgtgg cagcagagtg aagggaaagc gtactttacc
     2941 ggcgcaccaa cacgcgctgc gctgaaagtg tcattctttg gtcctttcta tggcggttat
     3001 aacgtcattg cacttgatcg ggaataccgc catgcgctgg tttgcggccc ggaccgcgac
     3061 tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca ggagatgctg
     3121 gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca gcagcccggt
     3181 agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc cagactcgcc
     3241 aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatagc cgcgccgact
     3301 gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt catcgcccag
     3361 gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg cgtcaaacga
     3421 ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag accagcaata
     3481 actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc ggtgaaaaga
     3541 cgcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc aggtggcagg
     3601 ggaatttcaa ctattacctt agtgactatc gcagaaaaaa caaacggcac gacacagctg
     3661 tcgtaccgtt attgcttatt gctattgctg cgctttcgtt gctgcgccag aaatcgcgtt
     3721 accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt
     3781 tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa cgtcttcctt
     3841 ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca tcgccaaaaa
     3901 tgacggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc gcgagagatg
     3961 gagttgccga gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca gcccgtgagc
     4021 agcgtactgg caagcaaaac aagaacgata aggcgtttca tcatctctga cctgaataag
     4081 tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga gacgtattca
     4141 ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg aaccttaacc
     4201 acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc acctttcagg
     4261 cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac ggagatcggc
     4321 tgaccattcc acagagttac gatacac
//
LOCUS       DAJTYD010000453         3353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603695_II
            SAMEA8901081-rid15816243.guided.252, whole genome shotgun sequence.
ACCESSION   DAJTYD010000453 DAJTYD010000000
VERSION     DAJTYD010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8901081
            Sequence Read Archive: ERR6005195
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478109.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478109.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:43:28
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,197
            CDSs (total)                      :: 4,140
            Genes (coding)                    :: 3,843
            CDSs (with protein)               :: 3,843
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 1 (5S, 16S)
            tRNAs                             :: 49
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 166 of 297
            Pseudo Genes (incomplete)         :: 76 of 297
            Pseudo Genes (internal stop)      :: 90 of 297
            Pseudo Genes (multiple problems)  :: 33 of 297
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8901081-rid15816243.guided.252"
                     /serovar="S. flexneri 6"
                     /isolate="603695_II"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-04-19"
     gene            complement(<1..553)
                     /gene="tetR(B)"
                     /locus_tag="OO585_004193"
     CDS             complement(<1..553)
                     /gene="tetR(B)"
                     /locus_tag="OO585_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6631565.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEP"
     gene            635..1840
                     /gene="tet(B)"
                     /locus_tag="OO585_004194"
     CDS             635..1840
                     /gene="tet(B)"
                     /locus_tag="OO585_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6631566.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1953..2546)
                     /gene="tetC"
                     /locus_tag="OO585_004195"
     CDS             complement(1953..2546)
                     /gene="tetC"
                     /locus_tag="OO585_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6631567.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2679..3050
                     /locus_tag="OO585_004196"
     CDS             2679..3050
                     /locus_tag="OO585_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6631568.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(3060..>3353)
                     /locus_tag="OO585_004197"
     CDS             complement(3060..>3353)
                     /locus_tag="OO585_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6631569.1"
                     /translation="GLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQ
                     ANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT          901 a          629 c          696 g         1127 t
ORIGIN      
        1 ctggctctgc accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac
       61 tatcagtagt aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata
      121 cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa
      181 aaccttgttg gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag
      241 gccgtgtacc taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac
      301 ttttagcgtt attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag
      361 tatggtgcct atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta
      421 catgccaata caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta
      481 aaccttcgat tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat
      541 ctaatctaga catcattaat tcctaatttt tgttgacact ctatcattga tagagttatt
      601 ttaccactcc ctatcagtga tagagaaaag tgaaatgaat agttcgacaa agatcgcatt
      661 ggtaattacg ttactcgatg ccatggggat tggccttatc atgccagtct tgccaacgtt
      721 attacgtgaa tttattgctt cggaagatat cgctaaccac tttggcgtat tgcttgcact
      781 ttatgcgtta atgcaggtta tctttgctcc ttggcttgga aaaatgtctg accgatttgg
      841 tcggcgccca gtgctgttgt tgtcattaat aggcgcatcg ctggattact tattgctggc
      901 tttttcaagt gcgctttgga tgctgtattt aggccgtttg ctttcaggga tcacaggagc
      961 tactggggct gtcgcggcat cggtcattgc cgataccacc tcagcttctc aacgcgtgaa
     1021 gtggttcggt tggttagggg caagttttgg gcttggttta atagcggggc ctattattgg
     1081 tggttttgca ggagagattt caccgcatag tccctttttt atcgctgcgt tgctaaatat
     1141 tgtcgctttc cttgtggtta tgttttggtt ccgtgaaacc aaaaatacac gtgataatac
     1201 agataccgaa gtaggggttg agacgcaatc gaattcggta tacatcactt tatttaaaac
     1261 gatgcccatt ttgttgatta tttatttttc agcgcaattg ataggccaaa ttcccgcaac
     1321 ggtgtgggtg ctatttaccg aaaatcgttt tggatggaat agcatgatgg ttggcttttc
     1381 attagcgggt cttggtcttt tacactcagt attccaagcc tttgtggcag gaagaatagc
     1441 cactaaatgg ggcgaaaaaa cggcagtact gctcggattt attgcagata gtagtgcatt
     1501 tgccttttta gcgtttatat ctgaaggttg gttagttttc cctgttttaa ttttattggc
     1561 tggtggtggg atcgctttac ctgcattaca gggagtgatg tctatccaaa caaagagtca
     1621 tcagcaaggt gctttacagg gattattggt gagccttacc aatgcaaccg gtgttattgg
     1681 cccattactg tttgctgtta tttataatca ttcactacca atttgggatg gctggatttg
     1741 gattattggt ttagcgtttt actgtattat tatcctgcta tcgatgacct tcatgttaac
     1801 ccctcaagct caggggagta aacaggagac aagtgcttag ttatttcgtc accaaatgat
     1861 gttattccgc gaaatataat gaccctcttg ataacccaag agggcatttt ttacgataaa
     1921 gaagatttag cttcaaataa aacctatcta ttttatttat ctttcaagct caataaaaag
     1981 ccgcggtaaa tagcaataaa ttggcctttt ttatcggcaa gctcttttag gtttttcgca
     2041 tgtattgcga tatgcataaa ccagccattg agtaagtttt taagcacatc atcatcataa
     2101 gctttaagtt ggttctcttg gatcaatttg ctgacaatgg cgtttacctt accagtaatg
     2161 tattcaaggc taattttttc aagttcattc caaccaatga taggcatcac ttcttggata
     2221 gggataaggt ttttattatt atcaataata taatcaagat aatgttcaaa tatactttct
     2281 aaggcagacc aaccatttgt taaatcagtt tttgttgtga tgtaggcatc aatcataatt
     2341 aattgctgct tataacaggc actgagtaat tgttttttat ttttaaagtg atgataaaag
     2401 gcacctttgg tcaccaacgc ttttcccgag atctcatcta ttgaaacagc ttgatagcct
     2461 ttttcaacaa acaatattcg tgctgagtta accagtgatt gataggtact cttaaaattt
     2521 tcttgttgat gatttttatt ttccatgata gatttaaaat aacataccgt cagtatgttt
     2581 atggtatcat gatgatgtgg tcgtgacaat cttaagaaca tttaggttat tttatgtata
     2641 ttgaacagca ttctcgctat caaaataaag ctaataacat ccaattaaga tatgatgata
     2701 agcagtttca tacaacggtt atcaaagatg ttctattatg gattgaacat aatttagatc
     2761 agtctttact gcttgatgat gtggcgaata aagcgggtta taccaagtgg tattttcagc
     2821 ggctgttcaa aaaagtaaca ggggtcacac tggctagcta tattcgtgct cgtcgtttga
     2881 cgaaagcggc tgttgagttg aggttgacga aaaaaactat ccttgagatc gcattaaaat
     2941 atcaatttga ttcccaacaa tcttttacac gtcgatttaa gtacattttt aaggttacac
     3001 caagttatta tcggcgtaat aaattatggg aattggaggc aatgcactga gagatcccct
     3061 cataatttcc ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc
     3121 acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
     3181 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
     3241 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
     3301 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcc
//
